LOCUS       DAWHRS010000001        40415 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWHRS010000001 DAWHRS010000000
VERSION     DAWHRS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.001"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(281..1078)
                     /locus_tag="WIB82_000001"
     CDS             complement(281..1078)
                     /locus_tag="WIB82_000001"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ3841382.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVNGVSTVLARLPLTAP
                     QIESEPESEPASQAASVETAANPSAEQRQIEATAAKIQAAVQDVLKD"
     gene            1233..4082
                     /gene="uvrA"
                     /locus_tag="WIB82_000002"
     CDS             1233..4082
                     /gene="uvrA"
                     /locus_tag="WIB82_000002"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ3841383.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPTVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLIL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIHGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIAKGSPEQVAKVKGSYTGKYLKVVLR"
     gene            4116..5711
                     /locus_tag="WIB82_000003"
     CDS             4116..5711
                     /locus_tag="WIB82_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="funZ protein"
                     /protein_id="HEZ3841384.1"
                     /translation="MKPLKTLEFGFVDAANYRRRENKDLFNRIFVKGEYLDELCEPNI
                     SFLIGEKGTGKTAYAVYLTNNFYKNIHATTKFVRETDYSKFIQLKKARHLTVSDFTSI
                     WKVILYLLISNQIKCKENGILSSIFNKFKALDEAINEYYYGAFDPEIVQAITLIENSK
                     EAAEMIFGKFVKLGEEESQQITFTESKFQANLGFIERKFKDALSQLKLKDNHILFIDG
                     IDIRPSQIPFDEYHECVKGLANAIWMLNNDIFPSIKDSKGRMRVVLLIRPDIFDSLGL
                     QNQNTKLQDNSVFLDWRTDYKSYRSSKIFGVFDHLLRTQQEKQDSLEKGNSWDYYFPW
                     NAPNLHDEYKNLTSFISFLRKSYYRPRDILQMLTLLQKNKKSKEDYVVAEDFDNTSFQ
                     REYSIYLLGEIKDHLLFYYSQSDYQNFLKFFEFLNGKDRFKYSDFLKAFERLKKHLQT
                     TSVEIPKFMSTANEFLQFLFDLNVIAYLDNPEDETKPYIHWCFKDRNYANISPKIKTE
                     TEYLIFSGLSKALDVGTPFKNKQ"
     gene            complement(6018..6233)
                     /locus_tag="WIB82_000004"
     CDS             complement(6018..6233)
                     /locus_tag="WIB82_000004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841385.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFWGSQRPLQNSPK
                     SPKFPPRHLGDFGEFCKGLGLSVPVCA"
     gene            complement(6262..7152)
                     /gene="sucD"
                     /locus_tag="WIB82_000005"
     CDS             complement(6262..7152)
                     /gene="sucD"
                     /locus_tag="WIB82_000005"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ3841386.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(7163..8329)
                     /gene="sucC"
                     /locus_tag="WIB82_000006"
     CDS             complement(7163..8329)
                     /gene="sucC"
                     /locus_tag="WIB82_000006"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ3841387.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(8428..8715)
                     /locus_tag="WIB82_000007"
     CDS             complement(8428..8715)
                     /locus_tag="WIB82_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841388.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(8840..10273)
                     /gene="lpdA"
                     /locus_tag="WIB82_000008"
     CDS             complement(8840..10273)
                     /gene="lpdA"
                     /locus_tag="WIB82_000008"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ3841389.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            10470..10583
                     /locus_tag="WIB82_000009"
                     /pseudo
     CDS             10470..10583
                     /locus_tag="WIB82_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(10638..11837)
                     /gene="odhB"
                     /locus_tag="WIB82_000010"
     CDS             complement(10638..11837)
                     /gene="odhB"
                     /locus_tag="WIB82_000010"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ3841390.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPA
                     EAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAPA
                     VALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKF
                     EKEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVP
                     ILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINP
                     PQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDP
                     ARLLLDL"
     gene            complement(11937..14765)
                     /locus_tag="WIB82_000011"
     CDS             complement(11937..14765)
                     /locus_tag="WIB82_000011"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ3841391.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGSADEAMLKKQVSVLRLISAYRIQG
                     VGAAKLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(14970..16253)
                     /gene="gltA"
                     /locus_tag="WIB82_000012"
     CDS             complement(14970..16253)
                     /gene="gltA"
                     /locus_tag="WIB82_000012"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ3841392.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSRVAEYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(16365..16613)
                     /locus_tag="WIB82_000013"
     CDS             complement(16365..16613)
                     /locus_tag="WIB82_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ3841393.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(16617..17324)
                     /locus_tag="WIB82_000014"
     CDS             complement(16617..17324)
                     /locus_tag="WIB82_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ3841394.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(17444..19207)
                     /gene="sdhA"
                     /locus_tag="WIB82_000015"
     CDS             complement(17444..19207)
                     /gene="sdhA"
                     /locus_tag="WIB82_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ3841395.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDANTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(19210..19551)
                     /gene="sdhD"
                     /locus_tag="WIB82_000016"
     CDS             complement(19210..19551)
                     /gene="sdhD"
                     /locus_tag="WIB82_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ3841396.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(19545..19922)
                     /gene="sdhC"
                     /locus_tag="WIB82_000017"
     CDS             complement(19545..19922)
                     /gene="sdhC"
                     /locus_tag="WIB82_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ3841397.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(20201..21604)
                     /locus_tag="WIB82_000018"
     CDS             complement(20201..21604)
                     /locus_tag="WIB82_000018"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ3841398.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(21853..22590)
                     /locus_tag="WIB82_000019"
     CDS             complement(21853..22590)
                     /locus_tag="WIB82_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ3841399.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(22869..25145)
                     /gene="metE"
                     /locus_tag="WIB82_000020"
     CDS             complement(22869..25145)
                     /gene="metE"
                     /locus_tag="WIB82_000020"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ3841400.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHANTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(25282..26160)
                     /gene="metF"
                     /locus_tag="WIB82_000021"
     CDS             complement(25282..26160)
                     /gene="metF"
                     /locus_tag="WIB82_000021"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ3841401.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            26362..26637
                     /locus_tag="WIB82_000022"
     CDS             26362..26637
                     /locus_tag="WIB82_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ3841402.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            26637..26762
                     /gene="ykgO"
                     /locus_tag="WIB82_000023"
     CDS             26637..26762
                     /gene="ykgO"
                     /locus_tag="WIB82_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ3841403.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(27024..27224)
                     /locus_tag="WIB82_000024"
     CDS             complement(27024..27224)
                     /locus_tag="WIB82_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841404.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            27367..28506
                     /locus_tag="WIB82_000025"
     CDS             27367..28506
                     /locus_tag="WIB82_000025"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ3841405.1"
                     /translation="MSQNASVDIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            28503..29084
                     /gene="metW"
                     /locus_tag="WIB82_000026"
     CDS             28503..29084
                     /gene="metW"
                     /locus_tag="WIB82_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ3841406.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRILERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            29201..30046
                     /locus_tag="WIB82_000027"
     CDS             29201..30046
                     /locus_tag="WIB82_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ3841407.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(30096..30656)
                     /gene="efp"
                     /locus_tag="WIB82_000028"
     CDS             complement(30096..30656)
                     /gene="efp"
                     /locus_tag="WIB82_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ3841408.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(30699..31847)
                     /gene="earP"
                     /locus_tag="WIB82_000029"
     CDS             complement(30699..31847)
                     /gene="earP"
                     /locus_tag="WIB82_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ3841409.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPLTLLLAGAQIIDS
                     LKQNGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRCLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(31866..32144)
                     /locus_tag="WIB82_000030"
     CDS             complement(31866..32144)
                     /locus_tag="WIB82_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841410.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
     gene            32324..33265
                     /gene="miaA"
                     /locus_tag="WIB82_000031"
     CDS             32324..33265
                     /gene="miaA"
                     /locus_tag="WIB82_000031"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ3841411.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            33269..33988
                     /locus_tag="WIB82_000032"
     CDS             33269..33988
                     /locus_tag="WIB82_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ3841412.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSCHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLNRFFQNKRKG"
     gene            33993..34481
                     /locus_tag="WIB82_000033"
     CDS             33993..34481
                     /locus_tag="WIB82_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ3841413.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTTFKIGCAVSILFILLVKYIAY
                     LF"
     gene            34547..35299
                     /gene="rlmB"
                     /locus_tag="WIB82_000034"
     CDS             34547..35299
                     /gene="rlmB"
                     /locus_tag="WIB82_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ3841414.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(35361..36752)
                     /locus_tag="WIB82_000035"
     CDS             complement(35361..36752)
                     /locus_tag="WIB82_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ3841415.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAMILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            37059..37934
                     /locus_tag="WIB82_000036"
     CDS             37059..37934
                     /locus_tag="WIB82_000036"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ3841416.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQVDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEN
                     GQAKVRAALKASGQL"
     gene            37943..39139
                     /gene="bamC"
                     /locus_tag="WIB82_000037"
     CDS             37943..39139
                     /gene="bamC"
                     /locus_tag="WIB82_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ3841417.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDKVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(39196..40128)
                     /gene="pip"
                     /locus_tag="WIB82_000038"
     CDS             complement(39196..40128)
                     /gene="pip"
                     /locus_tag="WIB82_000038"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ3841418.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEDVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT        10201 a        10866 c         9827 g         9521 t
ORIGIN      
        1 cgccttagca tttgggttct agattcccac tttcgtggga atgacggcgt aaaggtttct
       61 gttttttaag ttttaagtaa ctaatgagtt gtcattcccg cgcagacggg aatccaattc
      121 gttcggtttc agccgtttcc gataaatcgc tgtaacgtta agttcccgga ttcccatttt
      181 cgtgaacata acggtgtgaa cattagcaaa aattcaaaaa accaaacttc ctcattcgtg
      241 tgaatgatgg tactgatatt ttctatttta gttcgcaaaa ttaatctttc aacacatctt
      301 gcacagccgc ttgaatctta gccgccgttg cttcaatttg ccgttgttcg gcagatgggt
      361 ttgccgctgt ttcaaccgaa gccgcttgtg aagctggctc agattcaggc tcggattcga
      421 tttgcggcgc agtcagcggc aaacgcgcca atacagtgct gacgccgttt actttatcgc
      481 caatcgccac ttgcgcctgc gcatcgacag gcagatacat atccacgcgc gagccaaaac
      541 ggataaagcc atagcgttcg ccgcgagaca gtttcgcacc tgcttgggtg tagcacaaaa
      601 tacggcgcgc caccaaaccg gccacttgaa caaaagtaat ttcacgacct gaagccgtag
      661 tcgccaacac cgcattacgt tcgttttccg tgctggcttt gtccaaatcc gcattcacga
      721 atttgccttt gttatagacc actttcgtta ccgtacaatc ggcaggcgat ttttgcgaat
      781 gcacgttgaa cacgttcata aaaatactga ttttcaaagc atcgacatca cgatacggat
      841 cgcgtgcgcg ttcgaccacc acgatacggc cgtcaaccgg gctcaacacc gcttcaggat
      901 ttagcggaat ctcacgcgca gggtcgcgga aaaactgcaa tgcaaatacg gtaaacaccc
      961 aaaacggcaa agaccaccag ccgcaacata tcgacaccag caggctcaaa gccaaaccgc
     1021 cgccaataat cggccagccc tcacgggcga taatcgggtg ggggtaaaga cggttcattt
     1081 gtcatccttt tgttcaagcg ggttaaaatt gccgacatta tagcggaatc tgccgtactc
     1141 cgctggcaaa tgccgtctga acgcggcttc agacggcatc cgagaaagtg ctacaatacc
     1201 ggttttcctt tatttttcag gcggcatcca ttatgtgcaa ccatcatccc caacattcgc
     1261 acgacaacga caccatccgc atccgcggcg cgcgcacgca taatttgaaa aacatcgatt
     1321 tagacattcc gcgccacaaa ctcgtggtgg taacaggatt gtcgggcagc ggcaagtcgt
     1381 cgctggcgtt tgacacgctg tatgccgaag ggcaacggcg ttatgtcgag agcctttccg
     1441 cctatgcgcg gcagtttttg cagatgatgg acaaacccga tgtcgattta attgaaggtc
     1501 tgtcgcctgc aatttccatc gagcaaaaat ccaccagcca caacccacgc tccaccgtcg
     1561 gtacggtaac tgaaatccac gactatctgc gtcttttata cgcccgtgtc ggtaccccgt
     1621 attgtcccga acacaagctg ccgctgtcga gccaaaccgt atcgcagatg gtcgatgccg
     1681 tattgaaact gccggaagac acgcgtgtga tgattcttgc ccccaccgtg cgcgagcgta
     1741 agggcgagtt tgtcgatttc tttgccgact tgcaggcgca gggttttgcg cgggtgcgcg
     1801 tggacggcga ggtctatcag ttggacgaag tgccgaagct ggaaaaaaac atcaagcaca
     1861 atatcgacgt ggtcatcgac cgcgtgaaag tgaaggcgga catcaagcag cggctggcgg
     1921 aaagttttga aaccgcgctg cgccacggca acgagcgtgc gctggcgatg gagatggaca
     1981 gcggcgaaga acattggttt tccgcacgtt tcgcctgccc cgtgtgttca tacagcctgc
     2041 ccgaattaga gccgcgcctg ttttccttca acaacccgat gggttcctgc ccgacttgcg
     2101 acggcttggg caacaccaat ttcttcgacc ccgaaaaagt ggtcgcccac cccgaattgt
     2161 cgctggcttc gggcgcgatt gacggttggg acaagcgcaa ccagttctat ttccaaatga
     2221 ttcagtcgct ggcgcggcat tacggtttcg acgtgcaggc tgcttgggaa acgctacctg
     2281 aaaaagtgaa aaaagtcgtg ctgcacggct caggcaagga agtcattgat ttcacctacc
     2341 tatccgaacg cggcaccacc ttcaaccgca gccacgcctt cgaaggcatc atccccaatc
     2401 tcgaacgccg ctaccgcgaa accgacagcg aaaccgtgcg cgaaaaactg cgcgaatacc
     2461 aaaaccaccg cgcttgcccg agctgcggcg gcgcacgttt gcgcaaagaa gcccgctacg
     2521 tttacgtcgg cggcgagccg ttgcacgaag tttccgcctg gccgctgacc aaaacccacc
     2581 gattctttga aacgctggat ttggacggca acaaaaaaca aatcgccgaa aaaatcctca
     2641 aagaaatcac cgagcggctc ggctttttga ttaacgtcgg gctggattac ctgaatctct
     2701 cccgctccgc cgaaaccctt tccggcggcg aagcccagcg catccgcctt gccagccaaa
     2761 tcggcagcgg cctgaccggc gtgatgtacg ttttggacga accctccatc ggcctgcacc
     2821 agcgcgacaa cgaccgcctg ctcgccaccc tcaaacgcct gcgcgattta ggcaacagcg
     2881 tgattgtggt cgaacacgac gaagacgcca tccgcgaagc cgatttcgtc gtcgatatgg
     2941 gcccgggtgc gggtgagcac ggcggcaacg tactgattgc cgataccccc gaaaacgtcg
     3001 ccaaatgcga aaactccgtt accggacaat acctcagcgg caaaaaatcc attgccgtgc
     3061 cgtctgaacg cacgcccgtc aatcccgacc gaatgctcat cctcaaaggc gcgcgcggca
     3121 acaacctcaa aaacgttacc ctcgaactgc cgctcggttt gattacctgc atcaccggcg
     3181 tatccggcag cggcaagtcc actctgatta acgacaccct cgccaaaatc accgcccgcg
     3241 aactcaaccg cgcccaagaa gaacccgccc catacgacga catccacggt ctcgaacacc
     3301 tcgacaaagt catcaacgtc gaccaatccc ccatcggccg caccccgcgc tccaaccccg
     3361 ccacctacac cggcctgttc acccccatcc gcgaactctt cgccggtgta cccctctcgc
     3421 gcgaacgcgg ctacaacgtc ggcagattct ccttcaacgt caaaggcggc cgctgcgaag
     3481 cctgccaagg cgacggcgtc atcaaagtcg aaatgcactt cctgcccgac gtgtacgtcc
     3541 cctgcgaagt ctgccacggc aaacgctaca accgcgaaac gctcgaaatc caatacaaag
     3601 gcaaaaacat cagccaagtc ctcgacatga ccgtcgaaga agcccgcgaa tttttcgacg
     3661 ccgtccccac cgtatcgcgc aaactgcaaa ccctgatgga cgtaggtctc ggctacatcc
     3721 gcctcggcca atccgccacc accctctccg gcggcgaagc ccagcgcgtc aaactcgcct
     3781 tggaactctc caaacgcgac accggcagaa cgctctacat cctcgacgaa cccaccaccg
     3841 gcctacactt cgccgacatc gccctgctgc tggaagtcat aggccgtctg aaaggcaaag
     3901 gcaactcgat agtgattatc gagcataatc tggatgtgat taaaacagcg gattggattg
     3961 tggacttggg gccggaaggc ggagatggcg gggggaggat tattgctaaa ggtagtcctg
     4021 aacaggtggc gaaagttaag gggagttata ctgggaagta tttgaaggtg gttttgaggt
     4081 aagtttaatt caaaattaaa taggagatta ttaagatgaa accgttaaaa acattagaat
     4141 ttggatttgt ggatgctgca aactacagaa gaagagaaaa taaagattta tttaaccgaa
     4201 tatttgtaaa aggagaatat ttggatgaat tatgtgaacc aaatatttcg tttttaatcg
     4261 gagaaaaggg aactggaaag acagcatatg ctgtttattt aactaataac ttctataaaa
     4321 acatacatgc cactactaag tttgttcgtg aaaccgatta ttcaaaattt attcagctaa
     4381 agaaagcaag acacttaact gtttcagatt ttacaagtat ttggaaagtc attttatatc
     4441 tgttgatatc aaatcaaatc aaatgtaaag aaaacggaat attatcttca atatttaata
     4501 aatttaaagc cttagatgag gctataaatg aatattatta tggcgctttt gatccggaaa
     4561 ttgtacaagc aataacttta atagaaaatt caaaagaagc tgcggaaatg atttttggaa
     4621 aatttgttaa actaggtgaa gaggaatccc aacaaataac ttttacagaa agtaaattcc
     4681 aagcaaattt aggttttatt gaaagaaaat ttaaagatgc tttatctcag ttaaagctaa
     4741 aagataatca tattttgttt attgatggga tagatattag accatcacag attccatttg
     4801 atgaatatca tgagtgtgta aaaggtcttg ctaacgccat atggatgtta aataatgata
     4861 tcttcccttc cattaaagat agtaagggaa ggatgagagt tgtgttattg attagacctg
     4921 atatctttga ttcattaggt ttacaaaatc aaaataccaa acttcaagat aattcagtat
     4981 ttttagactg gaggacggat tataaatctt atagaagttc aaagattttt ggcgtttttg
     5041 atcatctttt gagaacccag caagaaaaac aagatagttt agaaaaaggc aactcatggg
     5101 attattattt tccatggaat gctcctaatt tacatgatga gtataaaaat ttaacttcat
     5161 ttattagctt cctaagaaaa tcgtattatc gacctcgcga tattcttcag atgcttactt
     5221 tgctacaaaa aaataagaaa agtaaggaag attatgtcgt agcagaagat tttgataata
     5281 cttcttttca aagagaatac tcgatatatt tacttggtga aatcaaagat catcttttgt
     5341 tttattatag tcaaagtgat tatcaaaatt tcctgaaatt ttttgaattt ttaaacggga
     5401 aagatagatt taaatatagt gattttttaa aagcatttga acgtttgaaa aagcacttac
     5461 aaacaacatc agtggaaata cctaaattta tgagtactgc taatgagttt ttgcaatttt
     5521 tatttgactt gaatgttatt gcttatttag ataacccaga agatgaaacg aaaccatata
     5581 tccattggtg ctttaaagat agaaattatg caaatatttc tcctaaaata aaaactgaaa
     5641 ctgaatattt aatattttca ggattatcaa aagcccttga tgttggtact ccatttaaga
     5701 acaaacagta acttgtatcc caaccaaccg tgtgcgctac gcacacggtt ggtagctgac
     5761 gtgttgcagt ttcaacagag aaagccgtag cctgcatacc caaccgaccg cgtgcgtggc
     5821 tgtgccacac gccctacctg tgaattacac ggaacttcaa agccaatacg agagctttgc
     5881 aaaattcctt tccctcccga cagccgaaac ccaaacacag gttttcggct gttttcgttt
     5941 caaatatcca ctaattttac cccaataccc ccttaatcct cccaggatac ccgataatca
     6001 ggcatccggg ccgcctttta ggcgcaaaca ggcacactta gcccgagacc tttgcaaaat
     6061 tccccaaaat cccctaaatg tcttggtggg aatttagggg attttgggga attttgcaaa
     6121 ggtctctgac tgccccaaaa gggcaaaaat aatgtgggta tgttgaggct gcctcttttt
     6181 gaaatgacaa acaaaatgcc gtctgaaacc cgatttccgg tttcagacgg catttgatgt
     6241 tcaatcagtt gtcaaacctg attatgccaa accttttgct ttcaacactt ccagcatggt
     6301 agtgcccaac tcggcagggc tgcgggtgta agcgataccg gctttttcga aagcggcgaa
     6361 tttttcttcc gcagtacctt tgccgccgga gataatcgca ccggcgtgac ccatgcgttt
     6421 gcctttaggt gcggtaacac cggcgatata gccgacaaca ggtttgctta cgttggattg
     6481 gatgtattcg gctgcttctt cttccgcagt accgccaatt tcaccgatca tgatgatggc
     6541 gtcggtatcc gggtcttctt ggaaaagttt cagtgcgtcg atttggttca tacccggaat
     6601 cgggtcgccg ccgataccga tacaggttga ttgacccaag cccagtttgg tggtttgtgc
     6661 cacggcttcg taagtcaatg taccggaacg ggaaatgatg ccgatgcggc cgggagtatg
     6721 gatgtggccc ggcataatgc cgattttgca ctcgcccgga gtaatcacgc ccgggcagtt
     6781 agggccgacc aaacgtgttc cgttaccgtt ggtttccaag tagcgtttgg ctttgagcat
     6841 gtccaaagtc ggcacgcctt cggtaatcac aacgaccaag cctacgcctg aatcaactgc
     6901 ttcaacgata gaatccaaca caaacggagc aggaacgtaa atcacggatg catccgcgcc
     6961 ggtttcttta acggcttctt tcatggtgtt gaacacgggc aggttcaggt gggtttgacc
     7021 gcctttgccc ggggtaacgc cgccgacaac tttagtgccg taagccagag cttgttcgga
     7081 gtggaaagta ccgtttttac cggtgaaacc ttgaaccaat actttagtgt ctttattaat
     7141 caatacgctc attcttttct ccttaggcgt ttacggctgc aacaattttt tcggctgcgt
     7201 cattcaggcc gtctgcagaa gtcagtttca gacctgattc gttcaggatt ttcgcgccga
     7261 gttcggcgtt gttgccttcc aaacgaacaa cgacaggaac gttgacgttg atttctttaa
     7321 cggctgccac gatggcttcc gcaatcatgt cgcaacgtac gataccgccg aagatgttga
     7381 tcaatacgcc ttgaacggat ttgtcttcca gaatcagttt gaacgcttca accacgcggt
     7441 ctttggttgc gccgccgcca acgtccaaga agttggcagg ttggccgcct ttcagtttga
     7501 tgatgtccat agtggccatc gccaaaccgg caccgttcac catacagccg atgttgcctt
     7561 ccagggcaac atagttcagg tcaaattcag aagctttcaa ctcgcgttcg ttttcttgag
     7621 atttgtcgcg caattcggcg attttcggca ggcggtagag cgcgttgctg tcgatgccga
     7681 ttttgccgtc cacgcaggcg agcgcgccgt tttcgcgaac tgccagcggg ttgacttcaa
     7741 acagggcgaa gtcattttcg acaaacgctt tgtacgcacc ggtcatcagt ttgacgaact
     7801 cgttgatttg tttgtctttc aagcccagtt ggaacgcaac ttcgcgagct tggcaaggtt
     7861 gcaggccgac cagcggatca acggttactt tgaagatttt ttcaggagtt tcggcagcaa
     7921 ctttttcgat ttccacgccg ccttcggtag aggccatgaa tgtaatgcgg cgggtagaac
     7981 ggtcaaccac tgcgcccaag tacagctcgg tttgaaccgg atacatgtct tcgcaaacca
     8041 aaacactgtt gacaggttgg ccgttggcat cggtttggta agttaccaag ttggtgccaa
     8101 tcaggctttc agccacttct ttagcttctt cgcggctttt aacgactttt acgccgcccg
     8161 ctttaccgcg gccgccggcg tgtacttgtg ctttgacaac agcgaatttg ccgcccaatt
     8221 tgtcgtaagc tgcagcggct tcttcgccgt tgtgtgccaa aataccgcct tgtacgggca
     8281 aaccgtagct agccagcagt tctttagcct gatactcgtg taaattcatg gatttctcct
     8341 ttagattagt ggaatgcccc gagtctgcct ccaaatggca agcccgtgcg tttcagacta
     8401 cctttcggcg gtcggaaaat cgtcgtttca cttgctgaaa ggcaaccaca tccgcgccag
     8461 cagatacatg gctgcaccgc ctcccccgag aataaaaaag acgatgcctt ttttacgcga
     8521 attcggacag agcagataaa cggcaaggct gaaactgata aacaacaatg ccacgcgcca
     8581 aaccaattct ttatctttga aacggttgag cgtataagtt tcggtcatca tcacatacat
     8641 ctgccacaat gcggccatac acatgctgat gatgcccgcc ggtataaaga aaataccgac
     8701 tatttctttg ttcataatga atgtaaatgc tgtttgtttg tctgacggaa ttataaatca
     8761 gaaaacggag ctctcggaac attcaatttg tcgaaggcct taaggccgtc tgaaaaattt
     8821 cagacggcct taatgtctat caaccgtgca aagcgcgttt gtcggccgcc aatgcagctt
     8881 cgtgaaccac ttcggacaag gttgggtggg catggataat gcgggcgatg tcttcgctgc
     8941 tggcgaagaa ttcgagcgca gtcacgcctt cggtaaccaa ttcgctgaca accggaccaa
     9001 tcatgtgtac gcccaagatg cggtcggttt tggcatctgc caacacttta accgtacctt
     9061 tggctttgcc cattgccaat gcgcgaccat tcgcaccaaa acctgaagta ccttttttgt
     9121 actccacgcc ttcggctttg agctgctctt cggttttacc cacccaagcg atttcaggat
     9181 cggtgtaaat cacgaacggt acgttgttga agtcgatatg cggtttttga ccggcaatgc
     9241 gttcggcaac ggcaacgcct tcgtcgctgg ctttgtgtgc caacatcggg ccgcgaacca
     9301 cgtcgccgat tgcccatacg ttaggcaggt tggtacggca ttcgccatct actttgataa
     9361 agccgcgctc gtctttttcc aagcctacgg cttccgcgtt caggcctttg gtgtttggaa
     9421 tacggccgat ggcaacgatc agtttgtcga atacttcggt tttggcttcg ccagcagcag
     9481 tttcgtaagc aacggaaaca cctttgcctt cagacttgat gtcgccgatt ttcacgccca
     9541 attcgatgct cagaccttgc tctttggtga agtatttgaa ggcttctttg gcgatttgtt
     9601 ggtcggcggc agccaggaag gtcggcgcgg cttcaagaat ggtaacttcc gcacccacgc
     9661 ggttccatac ggaacccatt tccaaaccaa tcacaccgga accgatcacg ccgagtttgg
     9721 caggtacttc ggtcaggttc aatgcgcctt cgttgtccaa tacgttcaca ttgtcgatag
     9781 cgacttgtgg cagcggacgc ggtacggaac cggtcgctac gatgacgtgt ttggcttcga
     9841 taacggtttt ctctcctttg ttatcgactt cgatttggta agcatcgcca tttttaccgg
     9901 caaaggaagc cgtaccgaac agactggtta ctttattttt ttggaacagg aatttcacgc
     9961 cgccggtcag tttggtcacg atggcatctt tgcgctcaat cattttggcc gcgtcgaatt
    10021 ttacgtcacc gacagtgata ccgtgttcgg caaaatcgtg ttgcgcagcg tggaaatgtt
    10081 cgctggattg caacagggct ttagaaggga tacaacctac gttcaagcaa gtaccgccca
    10141 atgcaggggc attgcctgct ttgttaacgc ctgcatcgac acaggcagtt ttgaaaccca
    10201 gttgtgcggc acggatggcg gcaacgtatc cgcccgggcc tgcaccaatc actactacat
    10261 catattgaga catcgtgtca tcctttgctt ttagcagtat ctcaaaataa aaacgatact
    10321 tttcgtttct tttttatttt gaatgactgc gttttgatgg ttttaagaaa aagccgtctg
    10381 aaagtccata tctgccgatt cattcgaatc aaatgtttca gacgacctca atcgttcgtt
    10441 gagcctattg tagcacgata ctacattacg ctacatctaa atatagtgga ttaaatttaa
    10501 atcaggacaa ggcgacgaag ccgcaggcag tacagatagt acggcaaggc gaggcaacgc
    10561 cgtactggtt taaatttaat ccactataaa aattcagacg gccttttcat taacaaataa
    10621 aaggccgtct gaaacgatta cagatccaac aacaggcggg ccgggtcttc caacgcgtct
    10681 ttaatggcta ccaaggtcaa tacagcttcg cgaccgtcga tgatacggtg gtcgtaagac
    10741 agagccagat acatcattgg acggacaaca acttgaccgt tttcaaccac agcgcgctct
    10801 ttagtggcat gcatacccaa aatcgcagat tgaggcgggt tgatgatcgg ggtagacatc
    10861 atagaaccaa aagtaccgcc gttggtaata ctgaatgtac cgccggtcag atcttcgata
    10921 gcgattttgc cgtctttggc ttttttcgcg taatcaacaa ttgcttgttc gatgtcggca
    10981 atgctcattt ggtcggcatc gcgcaggatt ggcacaacca aaccgcgtgg gctgccaatc
    11041 gcgataccga tgtcgaagta gccgtggtac acgatgtctt tgccgtcaac agaagcattc
    11101 acaaccgggt attttttcag ggcggcgaca gcggctttaa cgaagaagga cataaagccc
    11161 aatttcacgc cgtgttcttt ctcgaatttt tctttgtact tcgcacgcaa gtccatgatt
    11221 ggtttcatgt tgacttcgtt gaatgtagtc agaatggcgt tttcttgttg agaagccaag
    11281 aggcgttctg caacacgggc acgcaggcgg ctcattggta cgcgttcttc aggacgtgcg
    11341 ccggcaggaa gtgcaacggc aggggcaggt ttggctgcgg cattttgtac gtcttctttc
    11401 aatacgcgac cgtcacggcc ggaaccttgc aatgcgttca cgtcaacacc gctctctgca
    11461 gccagtttgg cggcggcagg catagcggcg ttgttttgtg cagcagcagg agcggcggcc
    11521 ggggcagctt cggcaggagc ggcggcaggt gcttcagcag cggcagtagc agcggcaggt
    11581 gcttcagcag cggcagtagc agcggtatcg atacgtgcca aaacttggtc ggcaacaacg
    11641 gtttcaccgt cttgcgctac gatttcaacc aatacgccgg cttgtggaga aggtacttcc
    11701 aaaaccactt tgtccgtttc gatatcgatc aggatttcgt cacgggcaac tgcttcgcca
    11761 acttttttct tccattccaa gagcgtgcct tcagatacgc tttcagacag cataggtact
    11821 tttacatcaa taatcatttt gtgtctccaa tggccctttc agacggcctg ttgtgtgttt
    11881 ttcattctgc atatgggtgt tccgcctgag cagcctcaga cggaatgcta cttggcttac
    11941 aatgccaaag cgtcttcaac caattgtttc aattgagcaa tgtgtttgct tgagtagccc
    12001 actgcaggcg atgcgctgct tggacgacca gcataagaca gtttttgctc ttcgctgata
    12061 acgtcttcga tgcggtggcg gatttggtag aacgcgcctt ggtttttcgg ctcttcttgc
    12121 gcccaaacca cagattttgc gttcggatat ttcgccagtt cggctttaac ctcatcgtat
    12181 gggaacggat acagctgctc aacgcggaca atcgcaacat catcttccag tttacgttcg
    12241 gctcgaccgg cttccaagtc atagtaaacc tgaccggcac acaataccac gcgtttcacg
    12301 ctgtcgttgc ttgcgcgttc ggcggtatcg ccgataaccg gacggaaagt cgaaccttcg
    12361 gtgaagtttt ccagcgggct cattgcccct ttgaatcgca acaggcgttt ggacatgaaa
    12421 attaccagcg gtttgcggta tgaacctaag acttgacgtt gcaagaggtg gaacatttgc
    12481 gacgcttcag atggcatgat gacttgcata ttgttctcag aacacagttg caaccaacgt
    12541 tctacgcgtg cagaagagtg ctcagggcct tgaccgtcgt aaccgtgcgg caggatggtg
    12601 gtcagaccgc acaagcgacc ccatttggtt tcgcctgaag acaggaattg atcaatcgtc
    12661 acttgcgcgc cgttggcgaa atcgccgaat tgcgcttccc aaatggtcag cttgtcaggt
    12721 gcggagcagg caaagccgta ctcgaacgcc atcacggcct cttcgttcaa aatggagtcg
    12781 ataaccagga actcgcccat tccttcgccc atatggcgca gaggaacata agtaccgtcg
    12841 tcccattttt cgcgtttttg atcgtgcaat acggcgtggc ggtgcgagaa cgtaccgcgg
    12901 ccggagtcct caccggagat acgcacgcca tgacctttgg ttacgaggct ggcgtatgcc
    12961 aaggtttcgg ccatacccca atctatggcc tgtttgccgg atgccatggc tttacgcgct
    13021 tcaatcacac gttttgcagt cggatgcagg gcaaagcctt ccggtacggc ggtaaacttc
    13081 tcagtgagac gctcaatatc tgcggcaggc aaaccggttt cgatgtgttc gcgccaatct
    13141 ttgccttggt atttgctcca gtcaatttgt gtgcgttgga agttgctcaa cgttgtttgc
    13201 tcgacgtgtt cgcctttatc caaagcatca cggtaggctt ggatgtaacc gtcagcctcg
    13261 gcttgggtta ccacgccttc ggcaatcagt tgctcggtgt acaaagcacg cgcacccggg
    13321 tgttgcgata cttttttgta catcatcggt tgggtcaagg tcggatcgtc gccctcgttg
    13381 tggccccatt tacggtagca gacaacgtca atcacgatgt ctttatggaa ttttttgcgg
    13441 taatccaaag cggcttggat agcaaagcaa acacgctcag gatcatcgcc gttcacatgg
    13501 ataaccgggg cggaaaccat ttttgcgata tcggtacagt gtacggttga acgggtatcg
    13561 cggatatcgg aagtggtaaa gccgatttgg ttgttgatga cgatatgaac cgtaccgccg
    13621 gtggtataac cgcgtgtttt agacaggttg aatgttgctt ggttgacacc cagaccgata
    13681 aatgcggagt cgccgtgaat caataccggc aagactttgt cgcggccgtt ttcgcccaaa
    13741 cgtttttgtt tggcgcgcgc agaaccttcc accaccgggt tgacgatttc caagtgtgac
    13801 gggttgaacg ccaaagaaac atgcatcggg ccgtgcgggg tagcaatatc ggagctgaag
    13861 cccatatggt atttcacgtc gccgctgggc agtttgattt cggcacgacc ttcaaattcg
    13921 gcaaacaaat cgccgggttt tttgcccaaa atgttcacca aaacattcag acggccacgg
    13981 tgcgccatac cgatgataac ttcttccaca ccgtctttac cggcgttttg aatcaggtag
    14041 ttcaaaccgg caatcgcgct ttcgccacct tcgacaccga aacgtttctg accgacgtat
    14101 ttggtatgca gataacgttc caaagtttcg gcagcggtca tttctttcag aatacggcgt
    14161 ttttgatcgg cattgtagtg cggcgtggac aatacgcttt caaaataatt gcgtacccaa
    14221 cggcgctctt cggtattggg aatatagata tattccaatg cgatgtgacc gcagtaggtt
    14281 tgtttgaggt tgctgatgat ttgggataaa ggcagtttgc cccgattggc aaaatcgccc
    14341 tcgcccatat tgaattggag cgccatatcg gcatctgaca gaccgtggaa tttcggatcg
    14401 agggcttcaa tatcgcgcgg cgggatacgt ttgagcggat caagtttggc tgcacccacg
    14461 ccttggatac gataagcaga aatcagccgt aaaacgctga cttgcttttt cagcattgcc
    14521 tcatccgcac tgcccgcaac ggcagatgca attttctttt tcgccaaagt aacaaatgat
    14581 tcgcgaatcg gtgtgtgtgc gacatcgaca gcaaccgtcc ccggctgttt gctcaaatcg
    14641 gtgaaatact gcttccattt ttcatcaacc gcatcggggt tttccaaaaa agcctcgtac
    14701 aattcctcaa tgtaaggtgc gtttgaaccg aacaggtaag agaaattgag tttttcgtcc
    14761 atcatggcgt gcgctctttt ctttaaaata taaacaaagg gtagaagtcc gcgtcctgac
    14821 ggctaaactg atgccgtctg aaaagacatt ttattctacc ctattttaaa ccggattgct
    14881 tgtcttgatt tcatccgact acaatcagac ggattcggga aacaatccgg ttctctgata
    14941 ttgcctgttt gacaatatat taatgtatgt tacctctcgc ctggcggcac ataatcgcgg
    15001 cgttccgaac cggtataaag ctggcgcggg cggccgattt tcagcgaagg atcgctaatc
    15061 atctcgtgcc agtgcgaaat ccagcccacg ctgcgcgaca gggcgaagat gacggtaaac
    15121 atttcggtcg ggatgcccag cgcggacagg acgatgccgg aatagaaatc gacattgggg
    15181 tacagtttgc gttcgataaa gaacgggtct ttcagcgcaa tctgttccaa ttccatcgcc
    15241 agcttgaatt tcggactgtc ttccaagccc aattccttca aaacttcata gcaggtttcg
    15301 cgcataatgc tggcgcgcgg atccatattg cggtacacgc ggtgtccgaa gcccatcaga
    15361 cggtatttgc gttgtttcac gccttccata tattcggcaa cacgggacac atctccgatt
    15421 tcatccaaca ttttcaacac ggcttcatta gcaccgccat gggacgcacc ccacaggcag
    15481 gcgataccgg cagcgataca ggcaaacggg ttcgcacccg aagagcctgc cagacggacg
    15541 gttgaagttg aggcgttttg ctcgtggtcg gcatgcaaaa taaagatgcg gtcgagtgcg
    15601 cgtgccaaaa cgggattggg tttgtagtct tcacacggcg tggcgaacat catatgaagg
    15661 aagttttcgg aataagaaag attattcttc ggataattga acggcagacc gtttgaatag
    15721 cggtagcaca ttgccgcaat ggtcgggatt ttggaaatca ggcggtaaat cgcgattttg
    15781 cggtgttcgg gattgctaat gtccaagctg tcttggtaga acgcagacag cgcgccgacc
    15841 acgccgacca tcatcgccat cggatgcgcg tcgcggcgga acccccggaa gaaccaagtc
    15901 agctgttcat gcaccatcgt gtggcggcgg acggtattgt caaattctgc cttttgctcg
    15961 ggagtcggca gttcgccgta aatcaacagg tagcagactt ccaaataatc ggacttttcg
    16021 gccagctgct cgatggggta tccgcgataa taaagcaagc cttgatcgcc gtcgatgtaa
    16081 gtaattttag actcacagct tgcggttgaa acaaatccgg ggtcgaagga aaacaaacct
    16141 gtattttttg tcagcccccg aatgtcaacc acatcgtgcc cgatgctggc ttccaatacc
    16201 ggcagctcca aacctgcctg acccggtacg ttgagtttga ttgatttgga catattattg
    16261 ctcctttgca agttataacc cacgttactg catccgcagc acgttctttg ccttcagacg
    16321 gcttttgcat tttgaaatct gcctgcaggc agatttcaga tgcctcatgc ccgtctgatt
    16381 ttttcaagca tcgggataag gtgccctttg tccgtttccg aatgcccgtt aatcaaggca
    16441 agcaattctt gatcttgaaa ttcaaggatt tcggaaaact cggacagctc tttatcgctc
    16501 aaatgctcga attctttttc cataaacctg ccgaagatta aatctaactc caacaatccc
    16561 cggcgggttt gaaaacggat tttccgtttg gcaatatcgt caaaaaccat catttcttaa
    16621 acggcccgtt tcaacataat ctctttaatc ttaccgatgg ctcgggtcgg attcaagtgt
    16681 ttgggacata cgtctacgca gttcataatg gtgtggcaac ggaacaaacg gtatgggtcg
    16741 ttcagattat ccaaacgctc attagtgatg gtatctcggc tgtccgcaat gaagcggtaa
    16801 gcattcagca aaccggacgg accaacgaat ttatcagggt tccaccaaaa tgacgggcag
    16861 gcagtcgaac agcaggcgca caaaatacac tcgtacaaac cgtccaactc tttacgctct
    16921 tcctgagttt gcagacgctc tttgtccgca tcaatcggat tatcgttgac aacataaggt
    16981 ttgacggaat ggtattgttt gaagaactgg gtcatatcca caatcaggtc gcggataaca
    17041 ggcagacctg gcagaggacg aattttaacc ggctgtttca agccacgcag atcggtcaaa
    17101 cacgccaagc cgtttttgcc gttgatgttc ataccgtcag aaccgcaaat cccttcgcgg
    17161 caggagcggc ggaaagacaa ggtatcgtct tgtgctttca ggcgcactaa agcgtccaaa
    17221 agtttcacgt cggtcggttc caattccaac tcgtaacgct gcatataagg cttggcatca
    17281 acatccgggt tgtaacggta aatttcaaaa ctcatttttt ccatgagaag tgttcctttt
    17341 ctcatcaact gcccaatgat tttgtattat aaattcaagc agttcaatgt gggtatggtt
    17401 agatttcaga cgacctttga ggcgaagacc gtctgaaaac gcatcaataa acgcgcttgg
    17461 ccggtttgat gtattccacg ctcaaaggct tggtgtgcac cggtttgtag gacaaggtat
    17521 tggcatctga atggtacagc gtatgtttca tccagttttc atcatcgcgc tcaggatggt
    17581 cgtctgaagc gtgcgcaccg cgtgattctt tacgtgcttc ggcagacacc aaagtcgctt
    17641 tcgccacttc aattaggtta tccaattcca aagcctcgat acgcgcggta ttccacactt
    17701 tgctcttgtc tttgatttcg gtacgtttca cacgctcggc aatcgccatg acttctcgaa
    17761 cgcctttgct cagaatctca tcagtacgga acacgccggc gtgcaattgt acggagcgtt
    17821 gcagttcgcg gcgcaatgca tcaacgtttt caccatcagt ttgattgtcc aaacgctcga
    17881 tacgttggcg ggtcagctcg cccgcatccg caggcaaagg tttccagtcg ctttgctctt
    17941 tgatgaattt aatcatgctg tcgccggcag ctttaccgaa taccactaag tccagcaggg
    18001 agttcgtacc caagcggttc gcaccgtgta cggaagcaca ggcgcactca cctgccgcat
    18061 acagaccttt tacaggcact tcgtattcgt cgccttgagg aacgacaact tcgccatggt
    18121 agttggtcgg aataccgccc atcatatagt gggtagtcgg cacaacggga atcgggtctt
    18181 taatcggatc gataccagcg aactgaatgg aaatctcacg gatgcccggc agtttttcca
    18241 taattttttc tgcgccgata tggtcgattt tcagtaagac atggtctttg tttttaccgc
    18301 agccgcgacc ttcgtagatt tccatcgcca tcgcgcggga aacaacgtcg cgagaagcca
    18361 agtcttttac ggtcggcgca tagcgttcca taaagcgttc gccgtcggca ttcaacagaa
    18421 taccgccctc gccgcgtacg ccttcggtaa tcaacacgcc cgcacctgcc acgccggtcg
    18481 ggtggaattg ccagaattcc atatcttcca acggaatacc cgcacgcgcg caaatgccca
    18541 aaccatcgcc ggtattcata taggcattgg tagaagaagc ataaatacga ccgccaccgc
    18601 cggtagcaaa catcacagct ttagcgtgga aaatataaac ttcgccggtt tccatttcca
    18661 tggcggttac gccgacgaca tcgccgtttt catcacgaat caaatcttgt gccgtccatt
    18721 ccacaaagaa ttgcgtattg gcacggacgt tttgttggta caaagtatgc agcatcgcat
    18781 gacctgtacg gtcggcaacc gcacaggcgc gttctaccgc gcgtttaccg tgttcggcag
    18841 tatggccgcc gaaaggacgc tgataaattt taccgctttc cacacggtca aaaggcatac
    18901 ccatgtgttc caactcaatt acggcttcag gcgcggcgcg gcacataaac tcaatcgcat
    18961 cttggtcgcc caaccagtcg gaacctttca cggtatcgta catgtgccag tcccaacggt
    19021 cttcctgcac attacccaga gaggcggaaa taccgccctg cgccgctacg gtatgcgaac
    19081 gggtcgggaa cactttagac aaaacggcac aattcagacc ggatttggat aattggaggg
    19141 ctgcgcgtaa acctgcacca ccaccgccga caatcacggc atcaaacttg cgaacaggaa
    19201 aacccatact tacccccaaa ttactttaat tgaatacacc aagcagccga ccagccagac
    19261 gatggtggca acctgcaaaa acaaacgcac gccgaagggt ttgatatagt ccatccacaa
    19321 atcgcggata cccacccaag cgtgcaagaa tacggcgatg aagctcactt gggtaaatac
    19381 ttttacccaa gtttgactaa aaaatgcctg ccatgccgaa tattctttag gcagggaaaa
    19441 tagaaccact aaaagtgcaa cggtataaat caacataata accgcagtcg cacgttgcat
    19501 cgcccaatcg cgcaaaccgt aatgggcacc ggtcaattta cgttctacca taacaacgct
    19561 cccaaaacga cagtcaaaac caatgcagaa gcaaatacgg ctttagcggt attgcgcgca
    19621 gtattcagct caaggccttt gtgcgcatcc aaaaataaaa agcggatacc ggcgagagaa
    19681 tggtgcagat aagcccacaa tacaccgatt aaaaccagct tgaccaaagg atgggaaaca
    19741 atggcacggt aagtttcaaa tgcagactct tgactcaggg taccggacag gaaatacagc
    19801 aggaaaggca gcataataaa cagcccgacc ccgctgatgc ggtgaaggat ggaaactatc
    19861 ccgggtatcg gcagacggat gttcggcaaa tccagataaa caggacgcgg tttggcagac
    19921 atagtcaatt cctctgtagt tatatatcga ctcaaaaacc gtaaacccat tgctacactg
    19981 ggttacataa acgttaattt acctgtttta tcccattttg aacagtgtat tttaaaaaca
    20041 aactgataga ttttgacaaa cacgcgtgga aacagcagat taaaagaaag atttttagtt
    20101 ttgccgcccc cttcccgaca cggtatcagc atccgacccc atccccaaaa acaaaatgcc
    20161 gtctgaaaaa atttcagacg gcattgtttt gccatatcgg tcaggctttc aatttcgcat
    20221 cggcatcgcg caacgcggta cgcagacctt cctcgataac gggatggtag aacggcatat
    20281 ccagcatttg cggaacggtc atcttcattt gatgcgccca tgccagcaag tgcgccaaat
    20341 gttcggcggc agggcctacg atttccgcgc cgataaagcg gccggtggct ttttcggcat
    20401 acaggcgcat atggcctttg ttcaccagca tcacgcggct gcggccttgg tttttgaacg
    20461 atacttcgcc gatgacaaat tcgtcggctt ggtattgcgc ggcaacctgc gcgtatttca
    20521 gaccgacaaa gccgatttgc ggactggtaa acaccacgcc gatggtgctg cgccgcaaac
    20581 cgccgccgat attcgggtag cggcccgcgt tatcgccggc aatcttgcct tggtcggcag
    20641 cttcatgcag cagaggcagt tggttggacg cgtcgcccgc aataaagata tgcggaatgc
    20701 tggtctgcat ggtcagtggg tcggcaacgg gtacgccgcg cgcgtctttt tcgatattga
    20761 tgttttccaa accgatattg tcaacgttcg gacggcggcc cacggctgcc agcatatatt
    20821 cggcaacaaa tacgcctttt tcgccgtcct gctcccaatg gacttctaca ttgccgtctg
    20881 catctagttt gacctcggtt ttagcatcca gatgcagttt caattcttcg ccgaacacgg
    20941 ctttcgcctc gtctgaaacg acggggtcgg aaatgccgcc gatgattccg cccaaaccga
    21001 aaatttcaac tttcacgccc aaacggtgca atgcctgacc cagttccaaa ccgataacac
    21061 ccggcccgaa cacggcaacg cgcttaggca gcgtatccca tgagaaaacg tcatcgttga
    21121 taatcaaacg attgcccaaa gactgccatt gcggcaggat gacgggacgc gaaccggtag
    21181 caatcacgaa actttttgcc gtaatttgag tatgctcgtc gatttggacg gtatgctcgt
    21241 cgataaattt agccgaaccc atgatgcgct tgtcggcagg ccactcttcc acatcggcaa
    21301 cgacaaagcc gacaaaacgg tcacgctcgg atttaacgcg ctgcatgacc tcttcaccgt
    21361 tgacgacgat gctgtctttg tccaaatgca cgccgaacgg gtcggtatgc aatgcgtggt
    21421 ggcgcgcttc tgcggcggca atcaagagtt tggaaggcat acaacccacg cgcgcgcagg
    21481 ttgtgccgaa cacattgttt tcaatcaggt aaacattatc cgaatgtaaa cgggcattgc
    21541 gaaacgcacc cataccggca gtaccgccgc cgattacgac gacatccgct tgaatttttt
    21601 tcataatctt tgtccttccg ttttcgtctg aaacattgcc gacacagaac atatcggttt
    21661 cagacggcat ttaaatattt tcccaatcaa aaacatcatt cagataaata aaaacaaaat
    21721 ttcgattgag tgcaacaata tccaaattct cttataaata tagagttatt cggatcggca
    21781 gtgtagaacc gcccgaataa ctctatccgg tatatcagac agtttaatca aaccgcctta
    21841 agcaaacagc tatcagtttt tagccaagta agcttccaaa tcttcgctgc cgccgatgta
    21901 tttgccgccg atgaagactt gaggggcagt catcttgccg gtaatggcgc gaacggaagt
    21961 aacggttgca tctttgccca atacgatttc ttcgtaagac aaacctttgt cttgcaaagc
    22021 ttgtttggct ttggcgcaga attggcaacc tggtttagtg aaaattgcca cagactcttg
    22081 agctttccaa tcaggagcaa cgaatttcag catagtatct gcatcggata ctttgaacgg
    22141 atcgcctggt tcttcaggct cgatgaacat tttttcaacc acgccgtcgt taaccagcat
    22201 ggagtaacgc caagagcgtt taccgaagcc caagtcttct ttaccgacca gcatacccat
    22261 accttcggta aattcgccgt tgccatcagg aatcatgtag atgttgtcgg attcttcttc
    22321 ggcagcccaa gcgttcatta cgaacgtatc gtttacagat acgcagtaga ttgcgtcaac
    22381 gccgttttct ttgaacgcgc cgaacaattc gttgtaacgc ggcaggtgtg aagaagaaca
    22441 agtcggggta aatgcaccgg gcagggagaa tacgactact tttttgcctt tgaacaaatc
    22501 atcggtagac acatctttcc aagtgtcgcc gacgcgggtg cggaatacta cggaaggtac
    22561 tttttgaccg gtacgatctt gcaaagccat tttaagctcc ttaatagatt ggtttgagaa
    22621 ataattgtga atcgctgggc attctacccc atgcaaaatg atttgtataa tttatagttc
    22681 aaataatata aattatttaa aactatcaaa cacaactccg atttgcctgt ttcccacata
    22741 taaagccgaa ttagaaaaaa acaagctgca atgaaaagat tttccggcaa ctgcgccctt
    22801 gcgccgcaaa tcaggacaaa ataccgtctg aaaggctttc agacggtatt tattcatacg
    22861 gtctcggctt atttcgccaa ttcggcacgc aatttgtggg ttacgttcat catcacttgg
    22921 agttgttcca gagtttcttt ccagccgcgt gttttcaggc cgcagtccgg gttaacccac
    22981 agacgttcaa ccggtacaac ctcgatggct ttgcgcaaca ggtgctccac ttcggcttct
    23041 gtcggtacgc gcgggctgtg gatgtcgtaa acccccgggc cgatgtcgtt cgggtatttg
    23101 aattcgccga acgcggtcaa gagttccatg tcggaacgtg aagtctcgat ggtgatcacg
    23161 tccgcatcca ttgcagcaat cgcaggcagg atatcgttga actcggagta gcacatatga
    23221 gtgtggattt gtgtgctgtc ttcgcaaccg gcagaggaca ggcggaagga ttcgcccgcc
    23281 cagttcaggt aggcatccca atcggcgcgt ttcagcggca agccttcgcg gatggcaggt
    23341 tcgtcaattt ggatgacttt gatgcctgct ttttccagat ccaatacttc gtcgttcaga
    23401 gccagtgcga tttgtttgca cacggtagag cgaggaatat cgttgcggac aaaagaccat
    23461 tgcagaatgg ttacagggcc ggtcaacata cctttcatcg ggcgtttggt caggctttgt
    23521 gcgtaagtag accaggcgac agtcatggct tcaggacggc ttacgtcacc gaagataatc
    23581 ggtggtttaa cacaacgtga gccgtagctt tgtacccagc cgtattgggt gaatgcaaaa
    23641 ccgctcaaca attcgccgaa gtattcgacc atgtcgttac gctcggcttc gccgtgtacc
    23701 agtacgtcca aatccagttt ttcttgttct tcaaccacca aggcgatttc ttttttcatc
    23761 gcggcttcgt aatcggcggc agacagttcg ccttttttga aggctgcgcg tgcttggcgg
    23821 atttcggtag tttgcgggaa agaaccgatg ttggtcgtcg gcagcagagg caggttcaac
    23881 catgcttgtt gcgctttgat acggtcggca aatggagatt tgcgttggtc tgcgttggca
    23941 ggcaaatcgg ccaggcgttt ggcaacgtct gcacggtgga tttcgctgct gttggcgcgt
    24001 gaatcggcag cagcttggct ggcggccagt tcttcggcaa cggaatcgcg accttcgttc
    24061 aatgcggctt tcagaacgcg caattcttgg gttttttgca gggtaaatgc caaccaagag
    24121 tacaggtccg gtttgttggc tttcagtttt tcttcaactg acaagtcaaa tggagtgtgc
    24181 agcagagagc aagagctgga aatccacaaa cggtcgccca gtttggcttg cagaggctca
    24241 acagtttcca aaactttgtt caggttggcg cgccagatgt tgcggccgtc gatgatgcct
    24301 gcagacagaa ctttgtcgta gtcggcaaat gcgtccagtt gctcgggagc gcgtaccaag
    24361 tcgatgtgca ggccgtcaac aggcagggat ttcaacaatg cggcgtgttc ggcaacagaa
    24421 ccgaagtaag tgctcagcag gattttggca cttactttgc tcaaagtagc gtaaacgtct
    24481 ttgtaggctt ctacccattc tttaggcaga tcgacagtca aagcaggctc gtcgatttga
    24541 atccactcgg caccggcttc aaccaaagca gtcaggattt caacgtaaac aggcaacagt
    24601 ttaggtaaca ggctcagacg gtcgaattca acggcgcctt tttctttacc aacccacagg
    24661 aaagtcaacg gacccacaac ggtcggtttg gctttcaagc ccaaagcttg ggcttcttgc
    24721 agttgttgga cgtagtgttt ggcgttggct ttgaattcag tattggcgtg gaattcaggc
    24781 accaagtagt ggtagttggt gtcgaaccat ttggtcattt cgatagcgaa ttggtctttg
    24841 ttaccgcgcg ccagttggaa gaattgttcc aaagacaggt tttggctgtc gaagccgaag
    24901 cgggcgggaa tcgcgccggt ggcgacttgc aggtcgagga tgtggtcgta gaaagtgaaa
    24961 tcgcctgcgg caacgtaatc ggcgttggca gcagcttggt gtttccagtt tttctcgcgc
    25021 aagtctttag caacagccag caattcttgc tcgctgattt ctttgcgcca gtatttttct
    25081 tgggcgaatt tcaattcgcg gaaggcgccg acacgcggga agcctgagaa atgtaatgtt
    25141 gtcatgttga actctccagt tggattttgt aaggaccggt tcaatccgaa tcggccggta
    25201 ttgtaaatca gaatggctag gatgggggcg gcgttttgct tttcagacgg cctcggatgc
    25261 cgtctgaagc cgttttcagc tttaagggcg cacgcctaaa atatggcaga tggcgtaagt
    25321 cagctcgctg cggttaagcg tatagaagtg gaaatctttc acgccttcgc gggacaggac
    25381 tttgaccata tcgatggcga tacttgccgc caccagattg cgcgtacctt ggtcgtcgtc
    25441 caaaccttca tacatttgcg acagccagct tgggattttg acgttggtta cttgagccat
    25501 tttaccgagc tgcttgaagt tggtaacagg caaaataccg gggacgattt ccacatcgat
    25561 acccaacatc acgcagcggt cgcggaagcg caggtagcgt tctacgtcaa agaaaaattg
    25621 ggtgatgacg tggttcgcgc ccgcatcgat tttgcgtttc aaattaatca ggtcggcttg
    25681 tgcggatttc gcttcgggat gcacttcggg atatgctgct acagagatgt cgaagtcggc
    25741 gacggagcgt aatagcttaa ccaagtcttc ggcataaaac ggttttttct cgtaaccggg
    25801 cggctcgtcg ccgcgcaggg cgacaatgcg gcggatgccg ctgtcccaat agtctttggc
    25861 gatttggcgc aattcgtcgg gagaagcgtc gatgccggtc aggtgcggcg cggcttccaa
    25921 gccggtttct tgcttaatgc gtttgacgat gccgtgcgtg cggtcgcgtt cgccggaatt
    25981 tgcgccgtag gttacggata cgaacttggg atgcagggtt tgcagacggt ggatggaatc
    26041 ccacagcatc gtttccattt gttcgttttt cggcgggaaa aattcaaacg aaacgttgat
    26101 gtcgcctttc aaatcggaaa ggctgttgtt taacgcattg atttcttttg cgtaattcat
    26161 ggctgcgtac cttttattat cttttatatg atattttcag gcaacataat aaagttgcgc
    26221 caaacatgag tcaatttcaa aattttcaga tttatcatga aaatttctaa tatattacaa
    26281 aaatagttaa atttccttgt acaagccttc taaatttatg ataaagtttg ttatgttata
    26341 acaattcaac taaggcgata tatgaaaccg aatattcatc ctgacaatta tcgaaccgtc
    26401 ctttttttcg acagcagcgc caatgaaggc tggttaatcc gctcctgtgc cgaaacacac
    26461 ggaaaaacga tggtttggac agacggcaaa gaatatccgc tgttttcgct ggatacttct
    26521 tccgcatccc atcccgtcta taccggcaag cagcgcaacg tcaacaccga aggccgtgcc
    26581 agcaaattca accaacgttt ccaatccgtc atgtcatcgt ttagaaagga caaataatgc
    26641 aggttctgtc ttcactcaaa actgccaaac aacgccaccg cgactgccaa atcgtcagac
    26701 gcaggggcaa agtttacgtc atctgcaaaa gcaatccgcg ttttaaagcg cgccaacgct
    26761 gaccccctac cccttcctgc ttcggttgta tagtggatta acaaaaatca ggacaaggcg
    26821 acgaagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt cagcacctta
    26881 gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggtttttg ttaatccact
    26941 ataaagcacc tcctaccgca agcccttttc atcaaatatg ccgtctgaaa cagaaaaatg
    27001 cgtttcaggc ggcatttttt ccgtcaatac gaatccggaa tgtgtttgaa atacagcata
    27061 aacagatacc aaaacagcgt cgcggcggcc gcaccgagca gcacccgggc actgatttcg
    27121 gcaacttcgc gcagggcgcg tttctgcctg cggctgaaga ggcggttgat aatcagcgcg
    27181 gcaagtgcga aaaagaaaaa aatacggaaa agtctgccta tcatatcggg aagcgaaaag
    27241 tatggttcgg gcgcggcagc atgggaaagg atattttagc gcatcgggct tcttgtccga
    27301 ccgtgtcaaa aagtcatgcc gtttgcgata taataacgct tttcgccgca tttgttggaa
    27361 caggatatga gtcaaaatgc ctcggtggac attgtaacgc cccaaaaaat tccgtttgag
    27421 atgccgctgg ttttggaaaa cggtaaaact ttgccgcgtt tcgatctgat gattgaaacc
    27481 tacggcgagc tgaatgccga aaaaaacaat gcggttttaa tctgccacgc gctgtcgggc
    27541 aaccatcatg ttgcgggcag gcattccgca gaggataaat atacgggctg gtgggacaat
    27601 atggtcggac ccggcaaacc gattgatacg gaacgttttt tcgtggtcgg tttgaataat
    27661 ctgggcggct gcgacggcag cagcgggcct ttgtcgatta atcctgaaac gggtagagaa
    27721 tacggcgcgg attttccggt ggttacggtg aaggactggg taaaatcaca agccgcgctt
    27781 gccgattatc tcggcatcgg gcaatgggcg gcggttgtcg gcggcagctt gggcggcatg
    27841 caggctttgc agtgggcgat ttcctatccc gaacgcgtgc gccatgcttt agtgattgcg
    27901 tctgcgccga agctgtccac gcaaaatatc gcgttcaacg atgtggcgcg tcaggcgatt
    27961 ttgaccgacc ccgatttcaa cgaaggacat taccgcagcc gtaacaccgt tcctgcacgg
    28021 ggcttacgga ttgcccgcat gatgggacac attacctatc ttgccgaaga cggtttgggc
    28081 aaaaaattcg gacgcgattt gcgctccaat ggctatcaat acggctatgg cgttgaattt
    28141 gaagtagaat cctatctgcg ctatcaaggc gacaaattcg tcggacggtt tgatgccaac
    28201 acctacctgc tgatgaccaa agctttggac tatttcgatc cggcggcgga tttcggcaac
    28261 agcctgaccc gcgccgtgca ggatgtgcag gcaaaattct ttgtcgccag cttcagcacc
    28321 gattggcgtt tcgcgcccga acgttcgcac gaactggtca aggcactgat tgccgcacaa
    28381 aaatccgtgc agtatatcga agtcaaatcc gcacacgggc acgatgcctt tttaatggaa
    28441 gacgaagcct atatgcgtgc ggtcgccgcc tatatgaaca acgtttataa ggaatgtcag
    28501 caatgaacct gcgcgatgat ttgcaactga tttacgaccg gatacccgaa ggcagccgcg
    28561 tcttggactt gggctgcggc gacggcgaac tgcttgccgc cttggtcgaa cacaaaaaat
    28621 gcagcggcta cggcatcgaa atcgacaccg acagcgtgat tgccgcgatg tcgcgcggcg
    28681 tgaatgttat ccaagccgat ttggaagaag gtttaaccgc attcaacgat caaagttttg
    28741 atgtgattgt gttgagccaa accatccaag cgatgcagaa taccgaaaaa atcctgcgct
    28801 gcctgatgcg cgttgccaaa caggcgattg tcagcttccc gaatttcggc tactggcgca
    28861 accgcgtcca aatcgcactc ggcggacata tgccggtttc cgaacgtatg ccctaccatt
    28921 ggtacgacac gcccaatatc cattggtgca cgctcaaaga ctttgattta ttgtgcgcca
    28981 aaaacaacat ccgcatcctc gagcgcgccg tgatgacggg caacagacag gtcaaacatt
    29041 tccccaacct tttgggcagt cttgcatttt accgcgtcgg gtaagacaga ttgagaacct
    29101 tgtcggcatt aacaggttct tcggttctat taacaacagg ccgtctgaaa aatgaaacgg
    29161 taggttttca gacggcctct ttaatactgg aaacaataga atgaaaaaca aaacctcatc
    29221 acttctctta tggcttgccg caatgatgct gaccgcgtgt tccccgagca aagaagataa
    29281 aacgaaagaa aacggcgcat ccgccgcctc gtccacggca tccgccgctt cgtcttccgc
    29341 gccccaaacc gatttgcaac cggccgcatc cgcccctgat aacgtcaagc aggcagaaag
    29401 cgtgccgccg tcaaattgca ccgacctgca ccccgccacc ggcattgacg atctcatgca
    29461 gcaaatcgcc gaacacattg actcggactg tctgtttgcc ctttcccatc acgaactgga
    29521 aacccgtttc ggcttacccg gcggcggcta tgacaacata cagcggctgc tgtttcccga
    29581 catccgccct gaagatcccg actaccatca gaaaatcata ctggcaattg aagacttgcg
    29641 ttacggaaag cgcacgatca gccggcaggc acaagatgcc ttgatggaac aggaacgccg
    29701 cctccgagaa gcgacgctgt tgctgataca gggcagtcaa gaaacccgcg gacaaggcga
    29761 ggagccgaaa cgcacgcgtt attttgaagt ttcggcaacc cctgcctatt cgagccggca
    29821 caacaacgga cttggcggca atttccaata catcggccaa ttgcccggct atctgaaaat
    29881 acacggagaa atgcttgaaa accaatcact cttccggctg tccaaccgtg aacgcaatcc
    29941 cgacaaaccg tttttagaca tccattttga cgaaaatggc aaaatcacgc gtattgtcgt
    30001 ttacgaaaaa aacatctact tcaatccaaa cttggggcga agataaaaaa atgccgtctg
    30061 aaacgttttc agacggcatt tttcaatgcg gcaaatcaag cgcgtttgcg gaattcgccg
    30121 gtgcgggtat cgatttcgat tttgtcgccg ttttcgatgt aagacatcac ttggatttcg
    30181 gtgcccccga ccaggcgcgc ggttttcatc actttgcctg aagtatcgcc tttgacggca
    30241 ggctcggtat actcgacttc gcgtacgatg atggtgggca gttcgacaga aatggggttg
    30301 ccttcgtaga aggtaacttc gcattggtct tccataccgt caacgatgaa tttcaacgcg
    30361 tcgccgatgt tgtcggcttc gatttcgtat tggttgaatt cttcatccat aaagacgtac
    30421 atcgggtcgg caaagtagct gtacgtacag tttttgcggg acaggatgac cacgtcgaat
    30481 ttgtcgtcgg ctttgtaaat ggtttcggaa gccgcgccgg tcagcaggtt tttcagtttc
    30541 atgctgactt tggcggaaga gcggccgcct ttgatgtatt cggttttttg aacgaccata
    30601 ggatcgttgc cgaccataaa tacattgccg gcgcgcagtt cttgtgctgt tttcattgtg
    30661 attttccaat caaatcggtt gtcgtaaaac gcactattct agcgtatttt ttgatgcttt
    30721 gaaacaaagg cggcgagttt ttcggatgcg gaaggctgcc cgaaaagata acggctccaa
    30781 tcctccgcgc cttgccgcca gccgttttga tgttgttgca ggatttgcca acattcgagg
    30841 cgttgcgtgg cggataaagc ccctccgccg tttaggtcgt ctgaaaggca gcggtgtgcc
    30901 gatgcggttt cgggcgtgta gaaaccgtgt gccttatccc aaaaggcgtg gagtttgtcg
    30961 agatggacat tctcgtcttg cggatagatg tgccaaaaga agggttttcc ggcaagctgg
    31021 gcgcgcacga aactgtcttc gccgcggatg acggcgcagt cggcaaggtg cagcagtttg
    31081 tcgaagtcct gttgcggcac gaaagggatt ttgacgaggc ggacggatgc cgtctgaaaa
    31141 acatcgccgt cgttttgcag ggcatcttgc ggaataacgc cgttttgttt gaggctgtcg
    31201 ataatttgcg cccccgccag caacagtgtc aacggactgc ctgcctgtcg ccacatttcc
    31261 agccactttg cccaaacatc gctccgatag ccgaaaagca gccattcggg ggcgtttttt
    31321 tcgggaagca tcagcctctt gcgcaaggct ccgctatcga aacggacggc ttcgcagtaa
    31381 tcacgttcgc gtatcaaccc gccgcttttt tcgctgaaac ccataaacca aaaatatttt
    31441 tgaacaccct cctgcggcga aggcatcaga tgcagccttt cattgctttc ctccgcgctc
    31501 aaatattccc aattcagcca aagcggcttg tgtcggcgga taatgtgcag cacattttcg
    31561 ggcaggtcgc aggcaaaagt ttcgatgacg acatcgggaa caggcgcggt atcaatatct
    31621 gccgcatcgg aatgccaagt gcggacatga atatcctgat gaacgcaggg aacatcgggc
    31681 aaatcagggc aaagcgcacg caaggcggac acatcgtccg tccacaaatg cacctgccaa
    31741 ccgagttcgc ggtgcaaaac acgggcgagc cgccacgaaa cgccgatgtc gccgaaattg
    31801 tcgatgacct tgcaaaaaat ccaacagaca aaaggaggag tattcataga aataacggaa
    31861 acaagctaaa acgcgccgaa atagtggaac aaatacacaa ccagcaaaac aatcggaata
    31921 aacagcaaca tggtttgacg tttcaaatca aactcttgtt tcaaatgatg cttgatggtg
    31981 tcgaaaagga cgaatttgaa caaaaacgtc gcttcgcagg caaggatgat gccgttgatc
    32041 agatgctggg gtttgtcttc gggcggattg gcgacgaaaa cggcaagtgt cgccaaaggg
    32101 atgaggcaga cgacaagcca gcggacaagc aggtttcggg acattgcaag gctttcaaaa
    32161 aagcaaaacg ccattatcgc cccaagcgca cgccttgtct aacggacgcg ccggcgatgc
    32221 cgtctgaagc cttgccgcag gaaaacgcag tgcttccgca aagcaaaata taagggcggc
    32281 gcgctttccg ctataatccg cattttcgca accctgaaac atcatgccga ccccgaaagc
    32341 ctttgccctg ctcgggccga ccgccggagg caaaaccgcg ctcgccctca aaattgccga
    32401 aaccctgcct gtcgaaatca tcagcctcga ttccgcgctg gtttaccgcg atatggacat
    32461 cggcacggcg aaaccgtccg cctccgaacg cgcctttgtc ccgcaccacc ttatcgacat
    32521 catcccgcct accgaatcat acagcgccgc ccgctttgtc gaagactgca cgcgcttggt
    32581 cggggaaatt tcctcacgcg gcaggtttgc ccttattgtc ggcggcacga tgatgtattt
    32641 ccgcgccctg acccaaggtt tgaacgattt gcccgaagcc gacgcctgcc tgcgtgccga
    32701 cttggacgaa caaaaacaga tgtacggctt ggatttcctc taccgcaccc tgcaaaaagt
    32761 cgaccctgaa accgcatgcc gtctgaaacc gaacgacagc cagcgtatcg aacgcgcttt
    32821 ggaagtttat tatctgaccg gcaaaccgat gagcgaacac ctcggcagac aaagccccca
    32881 tacgctcccc ttcgatttgc ataccgccgc cctgattccc gaaaaccgcg cccgcctgca
    32941 tgaaaacatc gccctgcgtt tccacctgat gcttgaacag ggctttatcg gcgaagtaga
    33001 aaacctgcgc cgccgctatc ccggcctgac cgccgactcc cccgccatcc gctgcgtcgg
    33061 ctaccgtcag gcatggaaat atctggatgg aaaaaccgat ttcccggcat ttgtcgagaa
    33121 aggcattgcc gccacccgcc aacttgccaa acgccaactg acttggttgc gcaaaacacc
    33181 tttagactgc gttgccgacc cgttttcaga cggcacttca tgcacacgcc tgattgaggc
    33241 ggcaaaacgg tttttcggcg cataaaccat gctgaccaca ccgccgctcg cccccaaaac
    33301 cgtcgccgcc ctgcacaggc tcggtatccg tacgctcgaa gaactgcggc aaaacggttc
    33361 ggtcaaagcc tttctgctgc tcaaagcatc gggtttgacc ctcacgaaaa gcacgctgtg
    33421 gcagcttgag tcgctgctga acggcacact cccgcaagaa atcacgcaac acgacaaaac
    33481 gcgccttttt gccgaattga aaaaccatcc gcccgtcgcc gcctttccgc cgcaggaaga
    33541 aatggaacac ttcatgcgcg aagcactgcg ccaagcagaa caatccgccg ctgacggcga
    33601 aatacccgtc ggggcagtca tcgtttcaga cggcaaaatc atcgcatcgg cacacaacac
    33661 ctgcattgcc gactgcaatg tcagctgcca cgccgaaatc aacgccttgg cacaggcagg
    33721 cagcgaaatg caaaactacc gccttgacgg atgcgacatc tacatcaccc tcgaaccatg
    33781 cgccatgtgc gcgtccgcac tgatacaggc acgaatcagg cgcgtgatct acggtgcggc
    33841 agaacccaaa accggcgcgg caggaagcat cgtcaacctg tttgccgaca aacgcctcaa
    33901 tacgcacacc gccatacggg gcggaatcct gcaagaagaa tgccgcgccg tattaaaccg
    33961 ttttttccaa aacaaaagaa aaggttgaac ctatgaccgc cttgctcgtc atcctcgccc
    34021 tcgccctgat agccgccggt acggcgggca tcgtttaccc cgccctgccc ggattggcat
    34081 tgatgtttgc cggaacctgg ctgctcgcct actccggcgg ctaccaaatc tacggcgcgg
    34141 gcgttttgtg gacggtcgga ctcatcagcc ttgccggcat actggcggac tatgtggcag
    34201 gcatatgggg gacaaaatat accggagcgg gcaagctcgc cgttcgcggc gcattggtcg
    34261 gcagcatcat cggcatattt ttctcccttc ccggactaat actcggtccc tttatcggcg
    34321 cggcggcagg cgaactgatc gatcggcgca atatgcttca ggcaggtaaa gcgggcttgg
    34381 gtacgctgtt ggggcttatc gtcggtacga cattcaaaat cggctgcgcc gtatccatct
    34441 tgtttatcct gttggtgaaa tacatcgcct acctgtttta accccttcag acggcatacc
    34501 ggcaaattgc tataatgccg tctgaaccct tttcacaaag caccatatgg caaaccaaag
    34561 acctatctac ggcttccatg ccgtcaacgc ccgattgtgg caaaacccca aatccatcgt
    34621 cgaactctat gtccaagaag gcaaaaacga tgtacgcacg cgcgaagtgt tggaaaaggc
    34681 ggcaaacgaa aacatccgcg tatattttgc cgatgccgac cgcctcaacg ccatcagcaa
    34741 aggtgcgcgc catcagggcg tggtcggatt tatcgatgcc tccaaaaacc acgtccacct
    34801 cgaagacgta ttggaaaact taagcgaacc gccgctgctg ctgatactcg acggcatcac
    34861 cgatccgcac aacctcggcg cgtgcctgcg taccgccgac gcaatgggcg tacatgccgt
    34921 catcgcaccg aaagacaaaa gcgcggggct gaacgccacc gtcagcaaag tcgccagcgg
    34981 cgcggcggaa accgtcccct acatcaccgt aaccaacctc gcccgcaccc tgcgcgagct
    35041 gaaagaatac ggcatttgga tcatcggcac cgatatgggc ggcaacgccg acctttacca
    35101 ttgcgatctg cccgacagcg cggcatgggt gatgggcaac gaaggcgacg gtatgcgccg
    35161 cctgacacgc gaacattgcg acatgctggt gtccataccc atgttcggca cggtcgaaag
    35221 catgaacgtc tccgtgtctg ccggaatggt attaagcgaa acccgccggc agcgcgtatt
    35281 aaaaaacgaa aaagcgtaag cctcaaaaac aatgccgtct gaaacaggga ttctgtttca
    35341 gacggcattc gttttcaaaa tcagtgttcc aagtggtcgg tatcaaactt gaccgccgcc
    35401 tcggttttat cttcgggcaa gaccaaattc agcaagactg ccgtaatgcc gccggcggaa
    35461 atagagtttt ggaacaagac aggcaggttt ttaaacactt ccggctcaaa cgccacgccc
    35521 aagcccaagc cgaccgaagt cgccgcaatc accgcctcgc gcctgcggat gccgtggctg
    35581 accaaaatcc gcacacccgc aatcgcaatc aagccgaaca tcaaaaccat cgcgccgcct
    35641 aaaaccggac tcggaatcgt cgtaaacgcg cgtccgacaa cggggaacag acccaacagc
    35701 accaaaatca tggcaatata tttgcccaca tggcgcgaag ccacgccggt catctgaatc
    35761 acgccgttgt tttgcgcaaa cgtcgtcagc ggcagcgaac ccaaagccgt cgcaatcact
    35821 gacaccaagc cgtcagccaa cacgccgccg cgcaggcgtt tggtgtattc ctcgccttca
    35881 atcggctggt cggacaccat tgccgtcgcc gtcaaatcgc cgaccgcctc aaacacgctc
    35941 aacaagaaaa tcgcacccgc cacaataaat gcgtgccagt caaaagcaaa accatattta
    36001 aacggtacgg gcagcgtaac cagcggcagg ttttgcagtg ccgaaaaatc caccttgccc
    36061 aaaaacagcg cgacgatata gccggcaatc agaccgaccg caatgccgct catccgcagc
    36121 agcgggtttt tcatgcagtt gaacaccagc acaatcagca gcaccagcga tgccagccct
    36181 aagttttcca tcgagccgaa cgtgccgtcc gccttcgcgc cgaagccgcc gccgaaatcg
    36241 gtaatgccga cgtgtaccaa actcaaacca atgagcatca cgaccacgcc gctgaccgtc
    36301 ggcgtaatca cttttttcaa atacggcaga agccacgccg agaaacacac caaaaacgcg
    36361 ccgacaaacg atacgcccaa gagcgtcgaa atcatcgcat ccttagtcaa accgccctct
    36421 ttcatccccg cgcccagcgc aatcatgacg gtaacgaacg agaaattcac cgactggatg
    36481 gacagcatcc ccgaaccgac cggcccgaag cggttgacct gcaaataagt gccgacaccc
    36541 gacgcaacca tcgccatcga cacgagatac gccgtcatct ccaccggcag ttccagcgcg
    36601 ccgcccacaa tcagcgcggg cgtaatcatc ggcacgaaaa tcgccaaaag atgggtaacc
    36661 gcgctcaaca gcgcattacc gaacggcggc ctatcctcca aaccgtacac caaatcaggc
    36721 gcatccgccc cttttcccat atgaccgctc ataatgtttc cttgcttcaa atgttgaaaa
    36781 aaccaaagcg cggattgtag cgaatccgcc ataccgcttc aatttcgatt tcggacggca
    36841 tacgcgcccg tccggcaaac agattccgaa ccgtcgccgc cggaatatcg tttacaaaac
    36901 gcaataacct gatttcaaac acaaaaatac aaacccgacg catccgccgt caagccgccc
    36961 cgctcatccc ggacccgccg gatttcacac ctgcggtaaa gtaggctaca atcggaagca
    37021 ttaccgcccc ctacaccaca actgataaaa ggttctgtat gttacaaggt agcctggttg
    37081 ccctgattac cccgatgaat caagacggca gcatccatta cgaacaactc cgcgacttaa
    37141 tcgactggca cattgaaaac ggcacggacg gcatcgttgc cgtcggcacg acaggcgaat
    37201 ccgccaccct ctccgtcgaa gaacacaccg ccgtcatcga agccgtcgtc aaacacgttg
    37261 ccaaacgcgt ccccgtcatc gccggcacag gggcaaacaa caccgtcgaa gccatcgccc
    37321 tttcccaagc cgccgaaaaa gccggggcgg actacaccct ctccgtcgtc ccctattaca
    37381 acaaaccttc gcaagaaggc atgtaccgcc atttcaaagc cgttgccgaa gccgccgcga
    37441 ttccgatgat actctacaac gtacccggca gaaccgtcgt cagcatgaac aacgaaacca
    37501 tcctgcgcct tgccgaaatc cccaacatcg tcggcgtgaa agaagccagc ggcaacatcg
    37561 gcagcaacat cgaactcatc aaccgcgcgc ccgaaggctt cgtcgtcctt tccggcgacg
    37621 accataccgc cctgccgttc atgctgtgcg gcggacacgg cgtgattacc gtagccgcca
    37681 acgccgcgcc caagctcttt gccgatatgt gccgcgccgc gctgcaagtc gacattgccc
    37741 tcgcacgcga actcaacaac cggctgattc cgatttacga caccatgttc tgcgaaccca
    37801 gccccgccgc accgaaatgg gcggtatccg ccttgggcag atgcgaaccg cacgtccgtt
    37861 tgccgctcgt tccgctgacg gaaaacggac aagcaaaagt ccgcgccgcc ctgaaagcct
    37921 caggacaact ttaaatatag ccatgccgtc cgaaccgttc ggacggcata acgcaacaaa
    37981 cactttaata tccatcacac aggatgacac gatgacccat atcaaacccg tcattgccgc
    38041 gctcgcactc atcgggcttg ccgcctgctc cggcagcaaa accgaacagc ccaagctcga
    38101 ctaccaaagc cggtcgcacc gcctgatcaa acttgaagtc ccacctgatt tgaacaaccc
    38161 cgaccaaggc aacctctacc gcctgcctgc cggttcgggc gccgtccgcg ccagcgattt
    38221 ggaaaaacgc cgcacacccg ccgtccaaca gcctgccgat gccgaagtat tgaaaagcgt
    38281 caaaggtgtc cgcctcgagc gcgacggcag ccaacgctgg ctcgttgtcg acggcaagtc
    38341 tcctgccgaa atctggccgc tcctgaaagc cttttggcag gaaaacggct tcgacatcaa
    38401 atccgaagaa cccgccatcg gacaaatgga aaccgagtgg gcggaaaacc gcgccaaaat
    38461 cccccaagac agcttgcgcc gcctcttcga caaagtcggc ttgggcggca tctactccac
    38521 cggcgagcgc gacaaattca tcgtccgtat cgaacagggc aaaaacggcg tttccgacat
    38581 cttcttcgcc cacaaagcca tgaaagaagt gtacggcggc aaagacaaag acacgaccgt
    38641 atggcagccc tccccgtccg atcccaacct cgaagccgct ttcctgacgc gctttatgca
    38701 atatttgggc gttgacggac agcaggcgga aaacgcatcg gcaaaaaaac ctacccttcc
    38761 cgccgccaac gaaatggcgc gtatcgaagg caaaagcctg attgtctttg gcgactacgg
    38821 cagaaactgg cggcgcaccg tgctcgccct cgaccgcatc gggctgaccg tcgtcggtca
    38881 aaacaccgaa cgccacgcct tcctggttca aaaagccccg aacgaaagca atgcagttac
    38941 cgaacaaaaa cccggcctgt tcaaacgcct gctgggcaaa ggcaaagcgg agaaacctgc
    39001 cgaacagccg gaactgattg tctatgcaga acctgtcgcc aacggctcgc gcatcgtcct
    39061 gctcaacaaa gacggcagcg catatgccgg caaagacgca tccgcattat tgggcaaact
    39121 ccattccgaa ctgcgttaaa ccattcaaaa caaaagcagc ttccaaacag aagctgcttt
    39181 tttatgcgga actttttaca acaaatgggg caaaatatcc tcaactgcct gaaccaacgc
    39241 atccaccaaa ggcggatcga acgcacgatg ccctgcctga accaccctca attccgcttc
    39301 gggaaaggct ttcgacagcg cccacgcact ctgcatcggc gtacacaaat cataccgccc
    39361 ctgcacgata atggtcggga tatgccgtat tttgccgata ttgttcaaaa tcgccctatc
    39421 gccctgcaac cagccgccgt tgacaaaata atggttttcc aaacgcgcaa tcgccagtga
    39481 ggcataagca tcttcatcca catcctccgg ctcgaaacgg atcagatagc tttcccaatc
    39541 cgcccaagcc ttcgcggcag acaggcacac ttcttcatct tgatgaaaca gcaacccgtg
    39601 atacgcctca atcagccggt tccgccgatt ttcagcaatc ggcgcgacaa atttttgcca
    39661 ttgttccgga taaatccggc tcacaccgcc cgcctcgttc agccacgccg tttcagacgg
    39721 cctgcacaaa aatatcccgc gcaataccaa tccctttacc cgttcaggat gggtttgggc
    39781 ataagccagc gacaaagtgc tgccccacga accgccgaac accagccatt tcctgatacc
    39841 cagcatttca cggacttttt caatatccgc caccaaatcc caagtcgtat tgtcttccgc
    39901 acaagcatac gggcgcgaac gtccgcaacc gcgctggtcg atgatgacga tacggaacac
    39961 atcgggattg aaaaaacccc gacattcagg cgaagccccc gcgcccggcc cgccatgtaa
    40021 aaaaatgacc ggcacaccgt cgggattgcc cgactcttcc caataaattt gatgaatttc
    40081 agacacctgc aagtatccgc tatgaaaagg ctgttttatc tcatacatca tcttctccca
    40141 tattgcttct gacaatccga acattcccac acgcaacatc acgcacgaga cacaagccgc
    40201 cccgaaaggc aaatgccgaa aaatcgcgat atagtgaatt aacaaaaatc aggacaaggc
    40261 ggcgagccgc agacagtacg gatagtacgg aaccgattca ctcgtgctta agcaccttag
    40321 agaatcattc tctttgagct aaggcgaggc aacgccgtac tggtttttgt taatccacta
    40381 taacagcaac cctgtcgccg tcattcccgc aaaag
//
LOCUS       DAWHRS010000002        37619 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWHRS010000002 DAWHRS010000000
VERSION     DAWHRS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37619
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.002"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            1..126
                     /locus_tag="WIB82_000039"
                     /pseudo
     CDS             1..126
                     /locus_tag="WIB82_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            390..1034
                     /gene="rplC"
                     /locus_tag="WIB82_000040"
     CDS             390..1034
                     /gene="rplC"
                     /locus_tag="WIB82_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ3841419.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1034..1654
                     /gene="rplD"
                     /locus_tag="WIB82_000041"
     CDS             1034..1654
                     /gene="rplD"
                     /locus_tag="WIB82_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ3841420.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1651..1965
                     /gene="rplW"
                     /locus_tag="WIB82_000042"
     CDS             1651..1965
                     /gene="rplW"
                     /locus_tag="WIB82_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ3841421.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANAAKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1971..2804
                     /gene="rplB"
                     /locus_tag="WIB82_000043"
     CDS             1971..2804
                     /gene="rplB"
                     /locus_tag="WIB82_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ3841422.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2811..3089
                     /gene="rpsS"
                     /locus_tag="WIB82_000044"
     CDS             2811..3089
                     /gene="rpsS"
                     /locus_tag="WIB82_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ3841423.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3098..3427
                     /gene="rplV"
                     /locus_tag="WIB82_000045"
     CDS             3098..3427
                     /gene="rplV"
                     /locus_tag="WIB82_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ3841424.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3437..4129
                     /gene="rpsC"
                     /locus_tag="WIB82_000046"
     CDS             3437..4129
                     /gene="rpsC"
                     /locus_tag="WIB82_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ3841425.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4113..4529
                     /gene="rplP"
                     /locus_tag="WIB82_000047"
     CDS             4113..4529
                     /gene="rplP"
                     /locus_tag="WIB82_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ3841426.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4529..4720
                     /gene="rpmC"
                     /locus_tag="WIB82_000048"
     CDS             4529..4720
                     /gene="rpmC"
                     /locus_tag="WIB82_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ3841427.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4720..4983
                     /gene="rpsQ"
                     /locus_tag="WIB82_000049"
     CDS             4720..4983
                     /gene="rpsQ"
                     /locus_tag="WIB82_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ3841428.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5205..5573
                     /gene="rplN"
                     /locus_tag="WIB82_000050"
     CDS             5205..5573
                     /gene="rplN"
                     /locus_tag="WIB82_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ3841429.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5585..5908
                     /gene="rplX"
                     /locus_tag="WIB82_000051"
     CDS             5585..5908
                     /gene="rplX"
                     /locus_tag="WIB82_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ3841430.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5918..6457
                     /gene="rplE"
                     /locus_tag="WIB82_000052"
     CDS             5918..6457
                     /gene="rplE"
                     /locus_tag="WIB82_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ3841431.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6460..6765
                     /gene="rpsN"
                     /locus_tag="WIB82_000053"
     CDS             6460..6765
                     /gene="rpsN"
                     /locus_tag="WIB82_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ3841432.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6781..7173
                     /gene="rpsH"
                     /locus_tag="WIB82_000054"
     CDS             6781..7173
                     /gene="rpsH"
                     /locus_tag="WIB82_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ3841433.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7187..7720
                     /gene="rplF"
                     /locus_tag="WIB82_000055"
     CDS             7187..7720
                     /gene="rplF"
                     /locus_tag="WIB82_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ3841434.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGAEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7734..8087
                     /gene="rplR"
                     /locus_tag="WIB82_000056"
     CDS             7734..8087
                     /gene="rplR"
                     /locus_tag="WIB82_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ3841435.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8105..8623
                     /gene="rpsE"
                     /locus_tag="WIB82_000057"
     CDS             8105..8623
                     /gene="rpsE"
                     /locus_tag="WIB82_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ3841436.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8616..8801
                     /gene="rpmD"
                     /locus_tag="WIB82_000058"
     CDS             8616..8801
                     /gene="rpmD"
                     /locus_tag="WIB82_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ3841437.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8803..9237
                     /gene="rplO"
                     /locus_tag="WIB82_000059"
     CDS             8803..9237
                     /gene="rplO"
                     /locus_tag="WIB82_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ3841438.1"
                     /translation="MFLNTIQPAEGATHASRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIAFGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9249..10559
                     /gene="secY"
                     /locus_tag="WIB82_000060"
     CDS             9249..10559
                     /gene="secY"
                     /locus_tag="WIB82_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ3841439.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLVVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10564..10782
                     /gene="infA"
                     /locus_tag="WIB82_000061"
     CDS             10564..10782
                     /gene="infA"
                     /locus_tag="WIB82_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ3841440.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10803..10916
                     /gene="rpmJ"
                     /locus_tag="WIB82_000062"
     CDS             10803..10916
                     /gene="rpmJ"
                     /locus_tag="WIB82_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ3841441.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10982..11344
                     /gene="rpsM"
                     /locus_tag="WIB82_000063"
     CDS             10982..11344
                     /gene="rpsM"
                     /locus_tag="WIB82_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ3841442.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11364..11759
                     /gene="rpsK"
                     /locus_tag="WIB82_000064"
     CDS             11364..11759
                     /gene="rpsK"
                     /locus_tag="WIB82_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ3841443.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11779..12399
                     /gene="rpsD"
                     /locus_tag="WIB82_000065"
     CDS             11779..12399
                     /gene="rpsD"
                     /locus_tag="WIB82_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ3841444.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12425..13411
                     /gene="rpoA"
                     /locus_tag="WIB82_000066"
     CDS             12425..13411
                     /gene="rpoA"
                     /locus_tag="WIB82_000066"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ3841445.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13435..13803
                     /gene="rplQ"
                     /locus_tag="WIB82_000067"
     CDS             13435..13803
                     /gene="rplQ"
                     /locus_tag="WIB82_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ3841446.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13950..14663
                     /gene="minC"
                     /locus_tag="WIB82_000068"
     CDS             13950..14663
                     /gene="minC"
                     /locus_tag="WIB82_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ3841447.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14691..15506
                     /gene="minD"
                     /locus_tag="WIB82_000069"
     CDS             14691..15506
                     /gene="minD"
                     /locus_tag="WIB82_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ3841448.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15510..15773
                     /gene="minE"
                     /locus_tag="WIB82_000070"
     CDS             15510..15773
                     /gene="minE"
                     /locus_tag="WIB82_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ3841449.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15777..16697
                     /gene="oxyR"
                     /locus_tag="WIB82_000071"
     CDS             15777..16697
                     /gene="oxyR"
                     /locus_tag="WIB82_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ3841450.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16694..16885)
                     /locus_tag="WIB82_000072"
     CDS             complement(16694..16885)
                     /locus_tag="WIB82_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841451.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            17271..17660
                     /locus_tag="WIB82_000073"
     CDS             17271..17660
                     /locus_tag="WIB82_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3841452.1"
                     /translation="MRYTIKTFQTLADETFPSKNFKILSFNGVARPITIVCPTHGVQT
                     VSAGQAFIRSKHGCPACGLVLSTSVLASRGRSVSILDTATGETLSFPSVQAAAKALNT
                     SYGSIRTKLDGRSSPDNLVCNRYKVMP"
     gene            complement(18030..20867)
                     /locus_tag="WIB82_000074"
     CDS             complement(18030..20867)
                     /locus_tag="WIB82_000074"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ3841453.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     NLDREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGRVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKNADSIMLARFPEADGGDIVQTVFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(20964..21773)
                     /gene="zupT"
                     /locus_tag="WIB82_000075"
     CDS             complement(20964..21773)
                     /gene="zupT"
                     /locus_tag="WIB82_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ3841454.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(21954..23210)
                     /locus_tag="WIB82_000076"
     CDS             complement(21954..23210)
                     /locus_tag="WIB82_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ3841455.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(23289..24680)
                     /locus_tag="WIB82_000077"
     CDS             complement(23289..24680)
                     /locus_tag="WIB82_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ3841456.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(25037..25813)
                     /gene="lpxA"
                     /locus_tag="WIB82_000078"
     CDS             complement(25037..25813)
                     /gene="lpxA"
                     /locus_tag="WIB82_000078"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ3841457.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFTQSTRGIIR"
     gene            complement(25856..26536)
                     /locus_tag="WIB82_000079"
     CDS             complement(25856..26536)
                     /locus_tag="WIB82_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ3841458.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(26743..27192)
                     /gene="fabZ"
                     /locus_tag="WIB82_000080"
     CDS             complement(26743..27192)
                     /gene="fabZ"
                     /locus_tag="WIB82_000080"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ3841459.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVS
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(27228..28271)
                     /gene="lpxD"
                     /locus_tag="WIB82_000081"
     CDS             complement(27228..28271)
                     /gene="lpxD"
                     /locus_tag="WIB82_000081"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ3841460.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEWRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVHIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            complement(28304..28804)
                     /locus_tag="WIB82_000082"
     CDS             complement(28304..28804)
                     /locus_tag="WIB82_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ3841461.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(28870..31263)
                     /gene="bamA"
                     /locus_tag="WIB82_000083"
     CDS             complement(28870..31263)
                     /gene="bamA"
                     /locus_tag="WIB82_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ3841462.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKIGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(31320..32660)
                     /gene="rseP"
                     /locus_tag="WIB82_000084"
     CDS             complement(31320..32660)
                     /gene="rseP"
                     /locus_tag="WIB82_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ3841463.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYIGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVV
                     GGVEKGSPADKAGLQPDDRLTAADGKPIASWQEWANLTRQSPGRKIALTYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(32695..33930)
                     /locus_tag="WIB82_000085"
     CDS             complement(32695..33930)
                     /locus_tag="WIB82_000085"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ3841464.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFQPEYAVVADAEYAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NSAAVLPVDSEHNAVFQVLPRDYTGRLNEHGIASIILTASGGPFLTTDLGTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(33935..34732)
                     /locus_tag="WIB82_000086"
     CDS             complement(33935..34732)
                     /locus_tag="WIB82_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ3841465.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(34735..35481)
                     /locus_tag="WIB82_000087"
     CDS             complement(34735..35481)
                     /locus_tag="WIB82_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ3841466.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(35537..36094)
                     /gene="frr"
                     /locus_tag="WIB82_000088"
     CDS             complement(35537..36094)
                     /gene="frr"
                     /locus_tag="WIB82_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ3841467.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(36274..37401)
                     /locus_tag="WIB82_000089"
     CDS             complement(36274..37401)
                     /locus_tag="WIB82_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ3841468.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHSVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(37477..37552)
                     /locus_tag="WIB82_000090"
     tRNA            complement(37477..37552)
                     /locus_tag="WIB82_000090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37517..37519),aa:Thr,seq:cgt)
BASE COUNT         8935 a         8514 c        10127 g        10043 t
ORIGIN      
        1 ttcaaagccg tcctgctcgg acaatggcac agcctctccg atcccgaact cgaacacagc
       61 ctcatcaccc gcatcgattt caacctgttt tgccgctttg acgaactgag catccccgat
      121 tacagtcatc aaccatattc cggtttgtcg gagaaagatg catacgctgt gatgaccgga
      181 taccgacccg ttaaaagagt ccgaccctat gccgtctgaa aattcaaaac gcttcagacg
      241 gcatattgaa gatatttctg atatttctgt tgatatttct ttgacttgtc agatataatg
      301 ccgagcttgg tacatttgtg ccaagtttaa ctttgtctga aagacaggcc aatcgtagcc
      361 tgtcccttta ctttaaaagg aaaataatca tgactttagg tctggttgga cgcaaagttg
      421 gtatgacccg cgtgttcgac gaacagggtg tttctgttcc ggtaaccgtt ttggatatgt
      481 ctgccaaccg cgttacacaa gtaaaatcca aagatactga cggctatact gccgttcaag
      541 ttacctttgg tcagaaaaaa gccaatcgtg tcaacaaagc cgaagccggg cactttgcaa
      601 aagcaggtgt tgaagccggt cgcggtttga ttgagtttgc tttgactgaa gaaaaactgg
      661 ctgaattgaa agctggtgac gaaatcaccg tttctatgtt tgaagtcggt caactggtcg
      721 atgtaaccgg tacctctaaa ggtaaaggtt tctccggcac gattaaacgt cataacttcg
      781 gtgcccaacg tacttcccac ggtaactccc gttctcaccg tgttccaggc tctatcggta
      841 tggcgcaaga cccgggtcgc gtgttccccg gtaaacgcat ggccggccaa tacggcaaca
      901 ccaaagcaac tgttcaaaaa ttggaagttg tccgtgttga cgcagaacgc caactgctgt
      961 tggttaaggg tgctgttccg ggtgcggtca acagcgatgt tgtagttcgt cccagcgtga
     1021 aagtaggtgc gtaatggaat tgaaagtaat tgacgctaaa ggacaagttt caggcagtct
     1081 gtctgtttct gatgctttgt tcgcccgcga atacaatgaa gcgttggttc atcagctggt
     1141 aaatgcctac ttggcaaacg cccgctccgg taaccgcgct caaaaaaccc gtgccgaagt
     1201 aaaacactca accaaaaaac catggcgtca aaaaggtacc ggccgtgccc gttccggtat
     1261 gacttcttct ccgctgtggc gtaaaggtgg tcgcgcgttc ccgaacaaac ccgacgaaaa
     1321 cttcactcaa aaagtaaacc gcaaaatgta ccgtgccggt atggcgacta ttctgtccca
     1381 attgactcgt gacgagcgtt tgtttgcgat tgaggcgttg actgccgaaa ctcctaaaac
     1441 caaagttttt gccgaacaag tgaaaaatct gggtctggag caagtgttgt ttgtaaccaa
     1501 acagctcgac gagaatgttt acttggcttc acgcaacttg ccaaacgtgt tggttttgga
     1561 agctcaacaa gttgatcctt acagcttgct gcgttacaaa aaagtaatca tcactaaaga
     1621 tgcagttgca caattagagg agcaatgggt atgaatcaac aacgtttgac tcaagtgatt
     1681 ttggcaccta tcgtttctga aaaaagcaac gtattggctg aaaaacgcaa ccaaatgacg
     1741 tttaaagttt tggcaaatgc agccaaacct gaaatcaaag cggctgtcga gctgctgttc
     1801 ggtgttcaag ttgcttctgt aactaccgtt acaactaaag gcaaaactaa gcgttttggt
     1861 cgtactttgg gccgccgcag cgatgttaaa aaagcttatg taagcttggc tgccggtcaa
     1921 gagttggatt tggaagccgc tgctgcagct gcagataagg aataaacaaa atggcaatcg
     1981 ttaaaatgaa gccgacctct gcaggccgtc gcggcatggt tcgcgtggta acagaaggtt
     2041 tgcacaaagg tgcaccttat gcacccttgc ttgaaaagaa aaattctact gccggccgta
     2101 acaataatgg tcatatcacc acccgtcaca aaggcggcgg tcataaacac cattaccgtg
     2161 ttgtagactt taaacgtaac aaagacggca ttcctgccaa agtagagcgt atcgaatacg
     2221 atcctaaccg tactgccttc atcgcactgt tgtgctatgc agacggcgag cgtcgttaca
     2281 tcattgctcc tcgcggtatt caagccggtg ccgtattggt ttccggtgct gaagctgcca
     2341 tcaaagtagg caacaccctg ccgatccgca acattcccgt tggtacgact atccactgta
     2401 tcgaaatgaa acccggtaaa ggtgctcaaa tcgcacgttc tgccggtgct tctgcggtat
     2461 tgctggctaa agaaggcgcg tacgctcaag tccgtctgcg ctctggcgaa gtccgtaaaa
     2521 tcaacgtaaa ttgccgtgcg accatcggtg aagttggtaa cgaagagcaa agcctgaaaa
     2581 aaatcggtaa agccggtgcc aaccgttggc gcggtattcg tccgaccgta cgcggtgttg
     2641 tcatgaatcc tgtcgatcac ccgcatggtg gtggtgaagg ccgtacgggc gaggcccgcg
     2701 aaccggtcag cccatggggt actcctgcta aaggctaccg cactcgtaat aacaaacgca
     2761 cggataacat gattgttcgt cgccgttact caaataaagg ttaatttagt atggctcgtt
     2821 cattgaaaaa aggcccatat gtagacctgc atttgctgaa aaaagtagat gctgctcgcg
     2881 caagcaacga caaacgcccg attaaaacct ggtctcgtcg ttctaccatt ctgcctgatt
     2941 ttatcggtct gaccattgct gtgcacaacg gccgcaccca tgtgcctgtg tttatcagcg
     3001 acaatatggt tggtcataaa ttaggcgaat tctcattgac ccgtaccttt aaaggccact
     3061 tggccgataa aaaggctaaa aagaaataag gtgaatcatg agagtaaatg cacaacataa
     3121 aaatgcccgt atctctgctc aaaaggctcg tttggtagct gatttgattc gtggtaaaga
     3181 cgttgcccaa gctttgaata ttttggcttt cagtcctaaa aaaggtgccg agctgattaa
     3241 aaaagtattg gagtcagcta ttgctaatgc cgagcacaat aacggtgcgg acattgatga
     3301 actgaaagtg gtaactatct ttgttgacaa aggcccaagc ttgaaacgtt ttcaagctcg
     3361 cgccaaaggt cgcggtaacc gcatcgaaaa acaaacttgt catatcaatg tgacagtggg
     3421 taactaagga aaagctatgg gacaaaagat taaccctaca ggctttcgcc tggcggtaac
     3481 taaagactgg gcttcaaaat ggtttgctaa aagcaccgac ttttctactg ttttgaagca
     3541 ggatatcgat gttcgcaatt atttgcgtca aaaattggcc aatgcttcgg ttggtcgagt
     3601 ggttattgaa cgccctgcaa aatctgcacg cattaccatt cactccgctc gtccgggtgt
     3661 ggttatcggt aaaaaaggtg aggatatcga ggttttgaaa cgtgacttgc aagtcttgat
     3721 gggtgtacct gttcatgtaa atattgaaga gattcgccgt cctgagttgg atgctcaaat
     3781 tattgctgac ggtattgccc agcagttgga aaagcgcgtt caattccgtc gtgctatgaa
     3841 acgagcaatg caaaatgcaa tgcgttctgg tgctaaaggt attaagatta tgacttcagg
     3901 ccgtctgaat ggtgcggata ttgcccgtag cgaatggtat cgtgaaggtc gcgtgccact
     3961 gcatacttta cgtgcaaatg tagattatgc aaccagcgaa gcgcacacca catatggtgt
     4021 attgggtctg aaagtttggg tttatacgga aggcaatatt aaatcttcca aacctgaaca
     4081 tgagagtaaa caaagaaagg caggtagacg taatgctgca gccaactaga ctgaaatacc
     4141 gtaagcaaca aaagggtcgc aatactggca tcgctactcg cggtaataag gtaagtttcg
     4201 gtgagttcgg cttgaaagcc gtaggtcgtg gtcgtttgac tgcccgtcaa atcgaagctg
     4261 ctcgtcgtgc aatgacccgt catatcaaac gtggtggtcg tatttggatt cgtgtattcc
     4321 ctgataaacc gattactgaa aagcctattc aagttcgtat gggtggcggt aaaggtaacg
     4381 tggaatatta cattgccgaa attaaaccag gtaaagtgtt gtatgaaatg gatggcgttc
     4441 cagaggaact ggctcgtgaa gcattcgagt tggctgctgc caaattgcct attcctacaa
     4501 cctttgtagt aagacaggtg ggtcaataat gaaagcaaat gaattgaaag acaaatccgt
     4561 tgagcagttg aatgcagatt tgttggactt gttgaaagct cagtttggct tacgtatgca
     4621 aaacgctacc ggtcaattag gcaaaccaag tgaattgaaa cgtgtacgtc gcgatattgc
     4681 tcgtattaaa accgttttaa ctgaaaaagg tgctaagtaa tgagcgaaac taaaaatgtt
     4741 cgtactttgc aaggcaaagt agtaagcgac aaaatggata aaaccgtaac agtattggtt
     4801 gagcgtaaag taaaacatcc gctgtatggt aagattattc ggttatctac taaaatccat
     4861 gcccatgatg aaaataatca atatggaatt ggtgatgtgg ttgttatatc ggaatcccgt
     4921 ccattgtcaa aaactaaatc ttgggttgtc agtgagctgg ttgagaaagc acgttctatt
     4981 taagaattaa agcaacgtgc ttggaatggg aaacgaagta ttgcagcaaa tttaatttgc
     5041 gtgtaaactt cgtttcctgt ctttcagttt cttctggaag tttcttccct ttcggggtcc
     5101 aagactggtt tacttgaacc gcaaggtttc atttaataag cagcggcttt gctgtaagtt
     5161 atctgaaagt ggtaaattaa gttggttaat ttaaaggtaa taacatgatt caaatgcaga
     5221 ccatcttaga tgtggctgat aactctggtg cgcgtcgcgt aatgtgtatc aaggtattgg
     5281 gcggatctaa gcgtcgctac gcttctgttg gcgatattat taaagtggca gttaaagatg
     5341 cggctccgcg tggccgtgtc aaaaaaggcg atgtatataa tgcggtagtt gttcgtactg
     5401 ctaagggtgt acgtcgtcct gatggtgcgt taattaaatt cgataacaat gccgccgtgt
     5461 tactgaataa taaacttgaa cctttgggta ctcgtatctt tggtccggta acccgtgaat
     5521 tgcgtactga gcgatttatg aaaatcgttt cattggcacc tgaagtatta taaggaatgg
     5581 cacgatgaat aaaatcatta aaggcgatag ggttgtagta attgctggta aggataaagg
     5641 taagcagggt caagtagttc gagtgttggg tgataaagtt gttgttgagg gcgttaatgt
     5701 tgtaaaacgc catcaaaaac ctaatccaat gcgtggcatt gagggcggta ttattactaa
     5761 agaaatgcct ttggatattt ctaatatcgc aatcctgaat ccggaaacta ataaagcgga
     5821 ccgtgttggt attaagctga ttgaaaatga aggcaaagtt aaacgcgttc gtttcttcaa
     5881 atcaaatggc tctatcattg gggcataagg agataacatg gctcggttga gagagtttta
     5941 taaagagaca gttgttcctg aattggttaa acaatttggt tacaaatcag taatggaagt
     6001 cccgcgtatt gaaaaaatta ccttgaatat gggtgtgggt gaggctgttg ctgataaaaa
     6061 agttatggaa catgctgttt ccgatttaga gaaaattgcc ggtcaaaaac cggttgttac
     6121 tgtcgcccgt aaatctatcg caggttttaa aatccgtgat aactatccgg ttggttgcaa
     6181 agtaacattg cgtcgtgatc aaatgtttga attcttggat cgtttgatta ctattgcatt
     6241 acctcgcgta cgtgacttcc gtggtgtgag cggtaaatca tttgatggcc gtggcaatta
     6301 caatatgggt gttcgtgagc aaattatttt tccggaaatt gaatacgata aaattgatgc
     6361 tttgcgtggt ttgaatatta ctattactac tacagcaaaa accgatgagg aagcgaaagc
     6421 tttattgtca ttgtttaaat ttccgttcaa aggataatca tggctaagaa agcacttatt
     6481 aatcgtgatc tgaaacgtca agctttggct aaaaaatatg cggctaaacg cgcggcaatt
     6541 aaagcggtaa tcaatgattc gaatgcaact gaggaagagc gttttgaggc tcgtttgagg
     6601 tttcaatcca ttcctcgtaa tgcggcacct gtgcgtcaac gtcgtcgttg tgctttgaca
     6661 ggtcgccctc gtggtacttt ccgtaaattt ggtttgggtc gtattaaaat ccgtgaaatc
     6721 gccatgcgtg gcgaaattcc gggtgttgtt aaagccagct ggtaatagga gtaattaaga
     6781 atgagtatgc atgatcctat ttccgatatg ttgactcgta tccgcaatgc gcaacgtgct
     6841 aataaagcag cagttgcaat gccttcttca aaattaaagt gtgctattgc aaaggtattg
     6901 aaagaagaag gatatattga ggacttcgca gtttcatctg acgtaaagtc tatattggaa
     6961 attcaattaa aatactatgc aggtcgtcct gtaattgaac aaatcaagcg tgtatctcgc
     7021 cctggtttgc gtatttataa agcgtctagt gagattccaa gtgttatgaa tggcttgggt
     7081 attgctattg ttagtacttc taaaggtgta atgactgatc gtaaagcacg ttctcaaggt
     7141 gttggtggtg agttgttatg cattgtagcc tagtggagga aaagaaatgt cacgtgtcgc
     7201 aaaaaaccca gtgactgttc ccgctggtgt agaagtaaaa tttggagcag aggcattagt
     7261 tattaagggt aagaacggtg aattgtcttt tcctttgcat tctgatgtag ccattgaatt
     7321 taatgatggc aaattgactt ttgttgcgaa taacagcagt aaacaagcaa atgcaatgtc
     7381 tggtactgct cgcgcattag tcagcaatat ggttaaaggt gtttcagaag gttttgagaa
     7441 aagattgcaa ttgataggtg tgggttatcg tgctcaagca caaggtaaaa tcttgaatct
     7501 gtctttgggt ttttctcatc cgatcgtata tgaaatgcct gaaggtgtct ccgttcaaac
     7561 tcctagccaa acagagattg ttttaaccgg ctcggataaa caagttgttg gtcaagttgc
     7621 tgctgagatt cgtgcgttcc gtgctcctga gccttataaa ggtaaaggtg ttcgctatgt
     7681 aggcgaagta gtggtaatga aagaagccaa gaaaaaataa ttgaggttca ctaatggata
     7741 aacatacaac ccgactccgt cgtgcacgca aaacccgtgc tcgtattgcg gacttgaaaa
     7801 tggtaagatt atgtgtgttc cgaagcaata atcatattta tgctcaagta attagtgctg
     7861 aaggtgataa agtattggct caagcctcta cattggaagc tgaggtgcgc ggtagtctga
     7921 aatctggaag caatgttgaa gcagctgcaa tagttggtaa acgtatcgct gagaaggcta
     7981 aagcagcagg tgttgaaaaa gttgcttttg accgttcagg tttccaatat cacggtcgtg
     8041 tgaaagcttt ggctgaagct gcacgtgaaa atggtttaag cttctaatat ttggagactt
     8101 tcagatggca aaacatgaaa ttgaagaacg cggtgacggc ctgattgaaa agatggtcgc
     8161 agttaatcgc gtaactaaag tagttaaagg tggccgtatc atggctttct cagcactgac
     8221 tgttgttggt gatggtgatg gtcgcattgg tatgggcaaa ggtaaatcaa aagaagtacc
     8281 agttgctgtt caaaaagcaa tggatcaagc tcgacgctct atgattaaag tacctttgaa
     8341 aaacggtact attcatcatg aggttattgg ccgtcatggt gctactaaag tatttatgca
     8401 gcctgctaaa gagggtagtg gcgtaaaagc cggtggacct atgcgtttgg tttttgatgc
     8461 tatgggcatt cataatatct ccgccaaagt gcacggatct actaacccat ataatatcgt
     8521 acgtgcaaca ttagatggtt tgtctaagtt gcatactcct gctgatatcg cagccaaacg
     8581 tggcttgaca gtggaagaca ttttgggagt taaccatggc tgaacaaaaa aagattaggg
     8641 ttacattggt taaaagcctg attggtacaa ttgaatctca tcgtgcatgt gctcgcggtt
     8701 taggtttgcg ccgccgtgaa catactgtag aggttttgga tacccctgaa aaccgtggca
     8761 tgatcaataa aatcagctac ttgttgaaag tggagtctta atatgttttt gaatactatt
     8821 caacctgctg agggtgctac tcacgctagt cgtcgtgttg gacgcggtat tggtagcggc
     8881 ttgggtaaga caggtggtcg tggtcataaa ggtcaaaaaa gccgttctgg tgggtttcat
     8941 aaggtgggtt tcgagggtgg tcaaatgccc ttgcaacgac gcctccctaa aagaggtttt
     9001 aaatctttaa cagcatcagc taatgcacag cttcgtttaa gtgaactgga atcaattgct
     9061 gttaatgaga ttgatatttt ggtcttaaag caagcgggtc tgattgcatc tacagtctct
     9121 aatgttaaag ttattgcttt tggtgaaatt tctaaggcag ttgctttgaa gggtattaaa
     9181 gttaccaaag gtgcgagagc tgctatcgag gctgttggtg gtaagattga aatgtaaggt
     9241 ttaatattgt ggctaatcaa caaacgtcat caggttcatc caaatttgga gatcttaaga
     9301 aacgtctttt gtttctattt ggagcattga ttgtttttcg aattggtgcc catatacccg
     9361 tacctggagt tgatgctgtt gctttagcta aattatacga aagcgctgga aacggcatcc
     9421 tgggaatatt gaatatgttt tccggtgggt cgttagagcg ctttagtata tttgcaatag
     9481 gaattatgcc atatatttca gcttctatta ttgtacagct cgcttctgaa attttgccat
     9541 cattgaaggc tttaaaaaaa gaaggggagg ctggtagaaa ggtaattacg aaatatacta
     9601 ggtatggtac tgttttgtta gcaattcttc aaagtctagg tgttgcatct ttcgtatttc
     9661 agcaaggaat tgttgtaaca agttcatttg agtttcatgt ttccacggta gtttctttgg
     9721 taacgggaac catgtttctt atgtggcttg gggagcaaat tactgaaagg ggtatcggga
     9781 acggtatttc tttaatcatt acggcaggta ttgcttcagg tattccttcg ggtattgcaa
     9841 agctggttac actgacgaac caaggttcta tgagcatgct tacggcgttg cttgttgtat
     9901 ttggtgcctt attattaatt tatttagttg tatactttga aagtgcacag cggaagattc
     9961 ctattcatta tgcaaaacgc cagtttaatg gtagggcggg tagtcaaaat acgcatatgc
    10021 ctttcaagtt gaatatggct ggtgttattc ccccaatttt tgcttccagt attattctat
    10081 ttccatctac tcttttaggt tggtttggtt cggctgatac aaatagtgtt ttgcacaaaa
    10141 tagctggatt gttacaacac ggtcaattgc tgtatatggc tttatttgca gcgacagtta
    10201 ttttcttttg ttatttttat acggctttgg tttttagccc taaagaaatg gcagagaatt
    10261 taaaaaagag tggtgctttt gttcctggga ttagacctgg tgagcagacc tctaggtatt
    10321 tagaaaaagt tgtattacgt ttgacattgt ttggagctct ttatattaca actatttgtt
    10381 taattccaga gttcttaact acggttttaa atgtaccttt ttatttgggt ggcacgtctt
    10441 tgttgattct agttgttgta acgatggatt ttagtacaca aataaattcg tataggctta
    10501 ctcaacagta tgataagtta atgactcgtt cagaaatgaa atcattttct cggaaataga
    10561 attatggcga aagaagatac tatccaaatg caaggtgaaa ttcttgaaac tttacctaat
    10621 gcaacattta aagtaaaact tgagaatgac catattgtat tgggtcatat ttctgggaag
    10681 atgcggatgc attacattcg tatttctccg ggagataagg tcacagtaga gctgacacct
    10741 tatgatttaa ctagggctcg aatcgttttc agagcaagat aaaccaataa aaggaaaata
    10801 aaatgcgtgt acaaccatct gttaagaaaa tttgccgaaa ttgcaagatt attcgtcgaa
    10861 atcgtgtagt tcgtgtaatt tgtactgatc tccgtcacaa acagcgtcaa ggttaatgga
    10921 atatttcttt taatgtgatt ctgtgatata gtgacacact ttgccctaaa aaggaaaaaa
    10981 tatggctcgt attgcagggg taaatatccc taataacgca cacatcgtaa ttggtcttca
    11041 ggctatttac ggtattggtg ctactcgtgc taaattgatt tgtgaggctg caaatattgc
    11101 gcctgatact aaagcaaaag atttggacga gactcaatta gatgctttgc gtgaccaagt
    11161 tgccaagtat gaagtagaag gtgatttgcg tcgtgaagta actatgagta tcaagcgatt
    11221 gatggacatg ggctgctatc gtggcttccg tcatcgtcgc ggcttaccat gccgcggtca
    11281 acgcactcgt acaaatgcgc gtacccgcaa aggtccgcgt aaagcgattg ctggtaagaa
    11341 ataaatttta aggaatttta ttaatggcta aagcaaacac agcttcacgt gtacgtaaaa
    11401 aagtacgtaa aaccgtgagt gagggtattg tgcacgttca tgcatctttc aacaatacca
    11461 tcattacaat cactgaccgt caaggcaatg cgttgtcttg ggctacctct ggcggcgctg
    11521 gttttaaagg ttctcgtaaa agtacaccat ttgcagcaca agttgcagca gaagcagctg
    11581 gtaaagttgc ccaagagtat ggcgttaaaa atttagaggt tcgtattaaa ggtccaggtc
    11641 caggtcgcga atcctctgta cgtgctttga atgctcttgg tttcaagatt accagcatta
    11701 ctgacgttac cccgttgcct cataacggtt gccgtccgcc taaaaaacgt cgtatttaat
    11761 attggagtga tttgaaacat ggcacgttat attggcccta aatgtaagtt ggcacgtcgc
    11821 gaaggtacgg atttgttttt gaagagtgcg cgccgctctt tggattctaa atgtaaaatt
    11881 gattccgctc ccggtcagca tggtgcaaaa aaaccgcgtt tgtcagacta tggtttgcag
    11941 ttgcgtgaaa aacaaaaaat ccgccgtatt tatggcgtat tagaacgtca gttccgtcgt
    12001 tatttcgcag aagctgatcg tcgtaaaggt tctaccggcg agttgctgtt gcagttgctg
    12061 gaatctcgtt tggataatgt cgtttatcgt atgggtttcg gttctacccg agctgaagca
    12121 agacagcttg tttctcataa ggcgatagtt gtgaatggac aagttgtcaa tattccttct
    12181 ttccaagtga aagctggtga tgttgtctca gttcgtgaaa aagccaaaaa acaggtacgt
    12241 attcaagaag cattgggttt ggcaactcaa atcggcttgc cgggttgggt ttctgtagat
    12301 gcggataaac ttgagggtgt gttcaaaaac atgccggatc gctcggaatt gaccggtgat
    12361 attaatgaac agctggtggt agagttctac tctaaataat gctagctcag tgagggacag
    12421 ttaaatgcag aatagcacaa ccgaattttt gaaacctcgt caaattgatg taaatacttt
    12481 ttctgcaact cgtgcaaaag tatctatgca gccatttgaa cgtggtttcg gtcatacctt
    12541 aggtaatgct ttgcgccgta tcttactgtc atccatgaat ggttttgctc ctactgaagt
    12601 agctattgcc ggtgtattac acgaatattc tactgttgat ggtattcagg aagatgttgt
    12661 tgacattttg ctgaatatta aaggtattgt gtttaaactc catggtcgta gccaagttca
    12721 acttgtgttg aagaaatcag gttcaggtgt cgtatctgcc ggtgatattg agttgccgca
    12781 tgatgtagaa attctgaatc ctggtcatgt catttgtcat ttggctgata acggtcaaat
    12841 tgagatggaa attaaagtag agcaaggtcg tggttatcaa tctgtttcag gtcgtcaggt
    12901 agttcgtgat gagaaccgtc agattggtgc aatccagttg gatgcgagct tttcgcccat
    12961 cagccgtgtt agctttgagg ttgaacctgc acgtgtagag cagcggacgg atcttgataa
    13021 gttggttttg gatatcgaaa ccgacggttc tattgatcct gaggaagctg tacgcagtgc
    13081 ggcacgtatt ttgattgatc agatgtctat ttttgctgat ttgcagggta cgcctgtgga
    13141 ggaggttgaa gaaaaagcac ctcctatcga ccctgttctt ttgcgtccgg tggatgatct
    13201 ggaattgaca gtacgttcag ctaattgttt gaaagctgag gatatttatt atattggcga
    13261 tttgattcaa cgcactgaaa ccgagcttct taaaacgccg aatttgggac gtaaatcttt
    13321 gaatgagatt aaggaagtat tggcatctaa aggtttgaca ctgggttcta agttggaagc
    13381 atggccacct gtaggcttgg aaaagcctta atgaagaatt aaaggataat tgatatgcgt
    13441 catcgtaatg gcaatcgcaa attaaaccgt accagcagtc atcgtgctgc aatgctgcgt
    13501 aatatggcga attcattatt gactcacgaa gctattgtaa caactctgcc taaggccaag
    13561 gaattgcgcc gtgtagtaga gccgttgatt acattgggta aaaagccgtc attggcaaac
    13621 cgccgtttgg catttgaccg tactcgcgac cgtgatgttg tagtaaaact gtttggcgat
    13681 ttgggtcctc gttttactgc tcgtaacggt ggttatgttc gggtgttgaa atacggattc
    13741 cgtaaaggtg ataatgcacc tctggcactg gttgaattgg ttgacaaacc ggctgctgag
    13801 taattttagt catataacgc catctgccga aaagcaggtg gcgttatttt tgcaatatct
    13861 gataggtaat agggtattgg ctatcatgtt taaaatatta attgaatagc taaggtttgc
    13921 gcggtaaact tacatcatta aaaaattcta tgatggttta tataatgaat gctttcgata
    13981 taaagtcgac aaagatggac gtattgtcta tatctttgca tacgtcagac ttgtttgatt
    14041 tggaagatgt gctggtcaaa ttgggcaaga agtttcaaga gtctggtgtt gttccatttg
    14101 tgctggatgt tcaagagttt gattatcccg agtctttgga tcttgctgca ttggtttcgt
    14161 tgttttcaag gcatggtatg caaattttgg gtctgaagca ttctaatgaa cgttgggctg
    14221 ctgcggctat gaagtatcat ttgctgtttt gtctgtctca ttcggaaaat gttaaagaac
    14281 tgggtcaggt tgaggtgcag aaaacggagg atggtcagaa agcaaggaaa acagtattga
    14341 ttacatcccc tgtccgtacc ggtcagcagg tttatgccga agatggcgat ttgattgtta
    14401 cgggggcggt cagccagggg gcggaattga ttgcggatgg caatatacat atttatgcgc
    14461 cgatgagggg gcgtgctttg gccggtgcca agggtgatac ttctgcccgc atatttatcc
    14521 actccatgca ggcagaactg gtttctgtgg cgggtattta ccgtaatttt gaacaggatt
    14581 tgccgaacca tctgcacaag cagccggtac agatattgtt gcaggataac cgattggtta
    14641 tcagtgcaat tggctcagag taattgtttg atatttaaaa aggaaatatt gtggcaaaaa
    14701 ttattgtagt aacttcaggt aagggcggtg tcggtaaaac gactaccagt gccagtattg
    14761 cgacaggttt ggcattacgc ggatataaaa ctgcggtaat tgattttgat gtgggtttgc
    14821 gtaacctcga cctcattatg ggttgcgagc gtcgtgtcgt ttatgacctg atcaatgtca
    14881 ttcaggggga ggcgacgctc aaccaagctt tgattaaaga taaaaattgt gaaaacctgt
    14941 ttattttgcc ggcttcccag actcgggata aagacgcttt gacacgcgag ggcgtagaaa
    15001 aagtgatgca ggagctgtcc ggcaagaaaa tgggctttga gtatattatt tgcgactctc
    15061 ctgccggtat tgagcagggt gcattgatgg cgttgtattt tgctgatgaa gccattgtaa
    15121 cgaccaatcc tgaggtttcc agtgtgcgtg actccgacag gattttggga attttgcaaa
    15181 gcaaatccca taaggcagag caaggcggtt cggttaaaga acatctgttg attacgcgtt
    15241 attctcccga acgtgtggca aaaggcgaaa tgctgtctgt acaggatatt tgcgatattc
    15301 tgcatattcc tttgctgggt gtgattcctg aatcccaaaa cgtcttgcag gcatccaatt
    15361 ccggagaacc ggtcatccat caggacagcg tggcggcttc cgaggcatat aaggacgtta
    15421 ttgcccgtct tttgggcgag aaccgtgaaa tgcgtttctt ggaagctgag aaaaaaagct
    15481 tcttcaaacg tctgtttgga ggataaggta tgtcattaat cgaattttta ttcggcagaa
    15541 agcagaaaac ggcaaccgtt gcccgcgacc gccttcaaat catcattgcc caagagcgcg
    15601 cccaagaagg tcaggctccg gattacctgc cgactttacg taaagagttg atggaagtcc
    15661 tgtccaaata tgtgaatgtt tcattagaca atatccgtat ttcccaggaa aaacaggatg
    15721 gtatggatgt gcttgagttg aacattactt tgccggaaca gaaaaaggta taggacatga
    15781 ccttaaccga attgcggtac atcgtcgcag tcgcccaaga acgtcatttc ggcagggcgg
    15841 cgcggcgttg ttttgtcagc cagcccactt tgtctattgc cattaagaaa ttggaagaag
    15901 agcttgccgt ctctttgttt gaccggagca gtaacgatat tattacgacc gaggcggggg
    15961 aacgtatcgt tgcacaggcg cgtaaggtat tggaagaggc ggagcttatc aggcatttgg
    16021 caaatgaaga acaaaacgag ctggagggtg cgttcaaact cgggctgatt tttacggttg
    16081 cgccgtacct gctgccgaaa ctgattgttt cgttgcgccg tactgcaccg aaaatgcctt
    16141 tgatgttgga agagaattac acgcatactt tgaccgagtc gctcaaacgc ggggacgttg
    16201 atgcgattat cgttgccgaa ccgtttcaag agccgggcat tgttaccgaa cccttgtatg
    16261 acgaaccgtt tttcgtgatt gtcccgaaag ggcattcatt tgaggaactg gatgccgttt
    16321 cgccacggat gctgggtgag gagcaggttt tgctgctgac ggaaggcaac tgtatgcggg
    16381 atcaggtact ctcaagctgt tccgaattgg cggcgaaaca acgtatacag gggttgacca
    16441 atacattgca gggcagctcg attaatacaa tccgccatat ggttgccagc ggtttggcaa
    16501 tcagcgtgtt accggcaacc gcgctgaccg aaaacgatca tatgctgttc agcattattc
    16561 cgtttgaggg tacgccgcca agccggcggg tcgtattggc gtaccgccgc aattttgtcc
    16621 gtccgaaggc gttgtcggcg atgaaggcgg cgattatgca gtcgcagctt cacggggtaa
    16681 gttttatccg cgactaggcg caggcattgt tttcaaaacg ccatttccct gagccgacaa
    16741 cacggtatgc caagatattg ccgtcatcat cgattttgag tatagcatcg ccacggaaac
    16801 tgccgtcctg aagatattcg acttttgcat cactgtgaat gttttcatca gtgccgatgc
    16861 aatgccatgt atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta
    16921 ttgccaaggc gtaccagcct gatttgttga tgtgtgtact actgcatggg ttgttaagcc
    16981 ctgcaagtga tttaagtgtg aagattgagt aatgcccact agtcgcaggc gcggggggca
    17041 ggtgctaagt cattggttat ctccttacta tctatctaac tacagggtgt attatgcgcc
    17101 tccttgttgt tttgtgcaag tggttttctg ataatttatg taaagatttg gtaaagacgt
    17161 atagcctttt tctttgcatt gtgcttgcgc agggtatcag gtaggtctca aaaaatttga
    17221 aaatttttat gttttcgtat aaaatctttt gcacttatac ggagtgagtt atgcggtaca
    17281 ccattaaaac atttcaaacc cttgctgatg aaactttccc ctctaagaac ttcaaaattc
    17341 tttcgttcaa tggggtggca cgacctataa ccattgtttg ccctacacac ggggtgcaga
    17401 ctgtttctgc ggggcaagca tttatccgtt ccaaacacgg ctgtcctgcc tgtggtttag
    17461 ttttatcaac ctccgttttg gcctctaggg gtcggtcagt ctccatcctc gacaccgcca
    17521 caggcgaaac actctcgttc cccagcgtac aagctgccgc caaagcccta aacacctcct
    17581 acggctctat acgaaccaag ctcgacggac gttcgtcccc cgacaacctt gtctgtaaca
    17641 ggtacaaagt aatgccgtaa tctcgaaact tcgtgctata ataaactcct agcttttgca
    17701 ggagtttgtt ttttatggat ttaaatgtcg aaatcaagaa ttttggcaag gttgcccatg
    17761 ctagggtttc cattcgcccg cttaccgtca tcacgggttg caacccacac tcgcgttccc
    17821 cagcgttact gccgcaggca ctgctttggg tgtgcatttt cagcaaatta accacaggct
    17881 caagggtcga acgtcctgtc aggaggtgtt gatttctaca ccgattatac tgtctgcggc
    17941 ttcgtcgcct tgtcctgatt tttgttaatc cactataaaa gaggccgtct gaaaaacatt
    18001 tttcagacgg ccttgtttat tcaatcaaat cagtctttca acttcgccaa ctgattttga
    18061 acttttgcca ttttgtcttc caattccgcc aaatcggctt tgtctttttc caccagatgc
    18121 gcaggggctt tttcggtgta gccgggtttg gagagtttgg cgttgagttt gtccaaggct
    18181 ttttgcagct tctcggcttc tttgctcaaa cgggcggttt cggcggcttt gtcgatttcg
    18241 actttcaaca tcaggcgcgc gccgttgcag acggcgacgg gcgcgtcttc gctttcgggt
    18301 agggcggcga cttgctgcgc ttcggtcagg cgggtcatca tcggcaggta tttgaggtag
    18361 tctgccaagt cgtccgcgct ttcgacaaac agcggagctt tgacattagg ctggataccc
    18421 atttcgccgc gcaggttgcg gactgcgcca atcaaatcct gcaatacggt catttgctcg
    18481 aataccgtct gaacaatatc gccgccgtcg gcttcgggga agcgggcgag catgatgctg
    18541 tcggcgtttt tcgcgtcgca cataggagcg acggtttgcc acagttcttc ggtaatgaac
    18601 gggataatcg ggtgcagcag gcgcagggcg gcttcgagta cgcgcaataa ggtatggcgt
    18661 gtggcgcgtt ggcggctggc gcagccggtt tgaagctgca ctttggcaag ttccagatac
    18721 cagtcgcaat agtcgttcca cacgaagctg tacagggttt ccgccgccaa atcaaagcgg
    18781 taggtttcgt aggcttgcgt tacttgctcg atggtctgat tcagacggcc tacaatccac
    18841 atatcgggga aggagtagcc gcgcggttcg gcagcggttg cgccgtaacc gcagtcttgg
    18901 ttttcggtgt tcatcaagac gaagttggtg gcgttccaga ttttgttgca gaagttgcgg
    18961 tagccttcgg cgcgtttgaa gtcgaagttg accgaacgcc ccaagctggc gtagctcgcc
    19021 atagtgaagc gcaaagcgtc cgcgcccata ctcggaatgc cttcggggaa gagttttttc
    19081 gtggcttctt ccactttcgg cgcggtttcg ggtttgcgca ggccggtggt gcgttttacc
    19141 agcagttttt ccaagccgat gccgtcgatc aaatccacag ggtcaatgac gttgccttcg
    19201 gatttggaca tttttttgcc ttcgtggtcg cgcacgatgc cgtggatata cacgtcttta
    19261 aacggtactc tgccggtgaa gtgggtggtc atcataatca tacgcgccac ccagaagaag
    19321 atgatttcgt agccggtaac caagacgttg gacggcagga aggctttgag ttcgtcggtt
    19381 tcagacggcc agccgagcgt ggagaacggc acgagcgcgg aggagaacca agtgtccaat
    19441 acgtcttctt cgcgggtcaa gcctgttttg ccggcttgtt tttcggcttc cacctgattg
    19501 cgggcgacgt aaacattgcc tgcttcgtcg taccacgcgg ggatttgatg cccccaccac
    19561 agttggcgcg agatgcacca gtcttggatg ttgttcatcc attggttgta ggtgttgacc
    19621 cagttttcgg ggataaagcg taccgcgccg ctgtcgacgg cttttttggc tttgtcggcg
    19681 aggctcaagc ctttgaattc gctgtcaggc tcgccgccgt ttggcgtagc ggacatggcg
    19741 acaaaccatt ggctggtcag catcggctca atcactgagc ctgttcggtc gcctttcggc
    19801 gtcatcagcg tgtgcgcttt gatttcgacc aagaaacctt gttcctgcaa atcggcaacc
    19861 atttgtttgc gcgcggcaaa gcggtctaag cctgcgtatt tttcaggcag ggcaaagctt
    19921 ggttgcgctt cgcctttgaa gttgaacact tcggcgtttg ccaatacttt ggcttccaag
    19981 tcgaacacat taaccaagcg cgtatcgtgg cgtttgccga cttcgtagtc gttgaagtcg
    20041 tgcgcaggcg tgattttcac gcagccggtg ccgaagtctt tttcaacgta ttcgtcggca
    20101 atcacgggaa tggtgcggcc ggtcagcggc aggattaatt ctttgccgat taagtgggta
    20161 taacgttcgt cttcgggatt gacggcaacg gcaacgtcgc ccaacagcgt ttcgggacgg
    20221 gtggtggcga cgatgacggc ttcggcagga ttgtccgcca gcggatagcg gatgtgccac
    20281 atagagcctt gttcttccac gctttccact tccaaatcag acactgcggt acccaatacc
    20341 ggatcccagt tcaccaagcg tttgccgcga taaatcaagc cctgttcata caggcgtacg
    20401 aacacttcgg ttacggtttc ggcgcgtaca tcgtccattg tgaaatactc gcgcgtccag
    20461 tcggcagaac agcccacacg gcgcatctgc tgcgtaatcg tgccgccgga aacttctttc
    20521 cattcccaca ctttttccaa gaatttttca cgatccaagt tatgacggga cacgttttgc
    20581 gcggcaagct gacgctcgac cacgatttgc gtggcgatgc ccgcgtggtc ggtgccggga
    20641 atccaggcgg tgttgcagcc tttcatacgg tagtagcggg tcaggccgtc cataatggtt
    20701 tggttgaagg catgacccat gtgcagcgtg ccggttacat tgggcggcgg cagttggatg
    20761 gaaaaagacg gtttggtcaa atccatatca ggttggaaat agccttggct ttcccagttt
    20821 tgatagtgct tggattcgat ttcggcgggg gagtatttgt ctaacatgat ggaactttgt
    20881 gaaattaagg ttatttttga tgtgcggatt ataacgcaaa aaggccgtct gaatcatttc
    20941 agacggcctt tggcatacag gttttaaaaa tggaacaata ccaggctgac ggcaatcacc
    21001 gccatgccca ttgtcaggcc gtaaacggtt tcatggccgt ctgaatagcg tttggcagcc
    21061 ggcagcagct cgtccaacgc caaaaacacc atcacaccgg ctatcacgcc gaataccgaa
    21121 ccaaacacgg caggcgacaa aaacggctgc aaaaccaaat agcccaaagc cgcccccaac
    21181 ggctcggcca agccggatag cagacacgcc cacaccgttt tcttacggct gcgggtggca
    21241 aaataaaccg gcgcggcgat ggaaatgccc tccggaatat tatggatggc aatcgccaag
    21301 gccaaaggca tcccgactgc tggattttcc aatgtggcaa aaaacgtcgc caagccttcg
    21361 gggaaattgt gcgcagtaat cgcaaacgcc gccatcatgc cgactcgcgc gatatggcgg
    21421 cgtttgcttt cttgaaacga cgggtcttgc gcgtctaaag tttcatgcgg gttcggcacc
    21481 agacggtcaa tcagcgcaat gccgcccatc ccggccaaaa atgccatggt cgccgccgca
    21541 aacgcgtggt ctttatcata aatttcagcg aacgcctcgc tggatttact gaaaatctcc
    21601 gtcagggaaa catataccat cgcaccgccg gcaaacgcca aaccaaacga caacacacgc
    21661 ggattgggcg ttttggaaaa catcaccaag ccactgccta atacggtaaa caaaccggca
    21721 gccaatgtga tggaaaaggc aacggccaaa ttggacatcg aaaaatcggg catgagaaaa
    21781 cctgcgctaa aagctgggac aggttcagac taacactttt taatgtatat gataatagtt
    21841 attatttatt ttattgattg gatacacgga ttttgaaaca aaaggccgtc tgaaaaatga
    21901 ttttcagacg gcctttaaat ttgaaatgcc gctaaacctt atcgctttcc agcttaagcc
    21961 tgataacgcg acaggctcaa atcgtcgctg cggatttcgg tgtctttgcc gctcacgata
    22021 tcggcggtta attttgccga acccagcgac atggtccagc ctaaagtacc gtggccggta
    22081 ttcagaaaca ggttgtcaaa gcgggtgcga ccgattaacg gcgtgctgtc gggcgtcatc
    22141 ggtctgaggc cgctccagaa cgatgcttgg ctcaaatcgc cgccttccgg gaacaagtcg
    22201 ttgacgacca aagccaaggt ttcgcggcgt ttttcgggca gtttgatttc gtagcccgac
    22261 aattccgcca taccgccgac gcggattctg ttgtcaaagc gcgtgatggc gactttgtag
    22321 ctttcatcta aaacggtgga caccggtgcg ccgtcggaat ttgtgaccgg cagggtcaag
    22381 gaatagcctt tgacgggata aatgggcaga ttgagatcca actgcgccaa aaccgtcctg
    22441 ctgaaacaac cgagcgcgca gacaacggca tctgcttcaa accgccctgt ttcggtttca
    22501 acggttttga tgcgcagccc gttgtggtcg atgcggctga tggtttggtt gaaatgaaac
    22561 cgtacgccct tttcctgaca caatttgtat aggttttcag tgaagaggcg gcagtcgccg
    22621 gttgcatccg caggcaggtg caggccgccg gcaattttgg cggtaacgcg tgccagcgca
    22681 ggctcgaatt ctgcgcattc ttcgggcttc agacggcggt acggcacgcc gtagcgttcc
    22741 aaaacggcaa tgtcttgttc tgcagcttca acttctttag cctgacggaa aatctgcaac
    22801 gtcccttttt tgcgcccttc aaaattcatg ccggtttgcg cttcaaaacg gcggaacatt
    22861 tcacggctgt attcggaaat cctgaccatg cgctctttat tggtttgata gtgcgctgcc
    22921 gtgcagtttt gcagcatccg ccacagccat tcgatttgat acaggctgcc gtcggggcga
    22981 aacagcaaag gcggatggct tttaaacagc catttcagtg ctttggtcgg aataccgggt
    23041 gcagcccaag gcgtggtata gccgtaagaa agctggcctg cattggcaaa actggtttcc
    23101 atcgccacac ccttggcgcg gtcgatgacc gttacttcat gtccggcctc tgccagatac
    23161 cacgcggaag acacgccggc aacacccgca cctaaaacaa gcactttcat gtttctccct
    23221 ccgacttttt caaaacagac ttaatatgcc gtgccgtctg aatattcgga ttcagacggc
    23281 ctcggatatt aatgcggcaa ttcgccgttt gtgatttttt gtttgaagtc gcgcgtttca
    23341 ttgacgatga ctttcgccat caataaaagt gcaatcaggt tgggcaatgc catcaagccg
    23401 ttgaatgtgt ccgaagccag ccacaccaaa tcaaggctca acacggtacc cagcataacg
    23461 gaagaaacat aaccgacgcg gtacaaaccg gcaaatttct cgccgaaaac atacaccgcg
    23521 catttttcgc cgtaatagca ccagcccaaa atggttgagt aggcaaagaa aatcaggccg
    23581 atggtaacaa tccagccgcc gatgccgggc agcatttttt ggaatgtgac ggttgtcagt
    23641 gccgcgccgc tcacttcagg tttgacaaac tcgccgcccg cgccgagcag tcccattacc
    23701 aacacgatgc cggtaatcga gcaaacgacg atggtatcca aaaacgtacc ggtcatagaa
    23761 accaaggcct gacggacggg atggtcggtt ttcgcggctg cggcggcaat cggtgcagaa
    23821 cccatgcccg cctcattgga gaacacgccg cgcgccacgc cgtagcggat gaccgtaccg
    23881 atagcgccgc ccgccactgc ctgcgcgctg aacgcatcgg agaaaatcag cttgacggca
    23941 ggcatcagtg catcggaatt aatcgcgata atggaaagac cgcccaacac ataaaacacc
    24001 gccatagcag gcacgatgaa agaagcggct ttggcgatgc ctttaatacc acctaaaacg
    24061 acaacggcag tcagaacggt caacgtaatg ccggtatagg caggttcgat accgaagctg
    24121 gtttgcaccg cctgtgcaac cgagttggac tgcaccgagc tgccgatacc gaaggaagcg
    24181 aatgtgccga acagcgcaaa cgcgacggcc atccatttcc agtttttgcc caagcctttt
    24241 tcgatgtaat acatcgggcc gccggacatt tcgcctttgg aattgttgac gcggtatttc
    24301 accgccaaca cgccttcgcc gtatttggtg gccatgccga aaatggcggt catccacatc
    24361 caaaataccg cgcccgggcc gccggttacc accgcagtcg ccacgccggc gatgttaccc
    24421 gtaccgatgg tggcggacag cgcggtcatc aacgccgcga aatgggaaat atcgccttcg
    24481 tggccttcgc cgccttcata cttctttgac ggcacaaatg cctgtttcag cgcatagccc
    24541 aacatagtga actgcaagcc tttcagcata acggtcagca caatgccggt gccgaccagc
    24601 agcatcaaca taacagatcc ccagacccag ccgctgacgg tttcgaaaaa ggctttgaga
    24661 ttgtctaaaa acaattgcat ggctttctcc tttgtctgtt ttatttttaa aacaccactt
    24721 ttgtagtgtc ctataatttc agcacagaat atccaataag acaatatgtt cttttgaaaa
    24781 atacttttgg ttttttcgcc gaaagcaggc cggttcaagc tgcggaaatt gtttgcaatt
    24841 ctttaaaagc agcggcggag gtcacaatga aatgtccgaa tggggatgtg gtcggcggca
    24901 gaaatcatca atgctgccga ctgccatact tctgaaatct acaaaatgat gcatcgatca
    24961 aacaatatac cgctttaaaa aaaccgatgc cgtctgaaac gctttcgggg tttcagacgg
    25021 catcaaaagg gtacggtcag cggatgatgc cgcgcgtcga ttgtgtgaaa aagtctcgga
    25081 atacggcaag ctcggcttgg gtttcggcgc ggcggaggat gtctgccttg gcctcttcaa
    25141 acggaatgcc gcgatggtag agggttttgt acacgtcttt gacggcggaa atctgttctg
    25201 cggtaaaacc gttgcggcgc atgccttcgc tgttgagccc cgccggttcg gcgcggtagc
    25261 ccgatgccat aaagtagggc ggtacgtctt tgtgtacgcc tgcggcgaag gcggtcatag
    25321 cgtagtcgcc gatgtggcag aattggaaaa ccagcgtgta gccgcccaaa acgacgtagt
    25381 cgccgatggt aacgtgtccg gcaagcgagg cgttgttggc gaagatggtg tggttgccga
    25441 ccacgcagtc gtgcgcgagg tggcagtacg ccataatcca gttgtcgtcg ccgatacggg
    25501 tttcgccgat gccggttacc gtgcctaaat taaaggtggt gaattcgcgg atggtgttgc
    25561 cgttgccgat aatcagcttg gtcggctcgt cgcggtattt tttgtcctgc gggatttcgc
    25621 cgaggctggc aaattggaaa atgcggttgt tttcgccgat gctggtgtgg ccgttgatga
    25681 cggcgtgcgg gccgatttcg gtattcgcgc cgatttggac gttggggccg ataacagtgt
    25741 acgcgccgac tttgacgctg gagtcgagtt cggctttggg gtcgatgacg gcggtcgggt
    25801 ggatgagggt catgttttga atactccgct gcttgttaag acaaataata gaaaattaca
    25861 gtttgtcgat ttcatcgcag accgcaatca cttgatcgct aaaatggcta aatactgaag
    25921 cagtatctac atccatgtaa ttaagcagtt caaaaataga ttggtgaata tttctagcgt
    25981 agttggtatc ttttattgca cctttaaaac aaatcggctc gtgatgtgcc aaacgattac
    26041 ggaatttgtt aatattggaa agttctcgaa aaatccattt ttggttatat tgcacacttg
    26101 gtgtagattt gggctttttt ggaaaaactt tcatcaatac tttccctgta gcatcaaatt
    26161 gggcatcttt ccctcctgca aacagatacc gccaaaaacc gaatcctagt cctgcaacca
    26221 attggttgtg gtcaaacttt cccccgctat tattttgtat tttagataat gcaaccttga
    26281 ttaattcagc agattctttc gtgccatttc ttaaaaagca accttgatat tttaatgcgg
    26341 gattcgtctg tggctgtatg ctgttatata accagtttct gtctttaaac gcctgctgaa
    26401 ggcaaatgtc aattttgttt cttaaaacaa tctcaaatat tgaaacaaca tgaaaaagtt
    26461 ctcttgataa agataaattc aataaataca attcagctgc tttgtcttcc ccaacggcat
    26521 ttttataccg ttccattctg aaatcagaaa aaatactaca gcattcctta taatccacgc
    26581 taacacctga ttctttattt ttaaattgac gtttcaaact atatttgata cagttaggta
    26641 aaactaatcc ccaccgtgtc cctgggtttg aataaaccta aaccggtggg ttttcttttt
    26701 attaggtcgt ctgaaacttt cagacgacct ttttccgaac gctcaaacca cgcgtttggc
    26761 gcacataatc acggcttcaa cggcaacttg cccgtccact ttggcgacgg cgttgaattt
    26821 gccgatgccg cgccggctgg tcagcagctc gacttcaaag acgagttggt cgccggggat
    26881 gacttggcgt ttgaaacggg cttcgtctat gccggcgaag aagaagaatt cgttttcttt
    26941 gcgcccgcct tcgctcaaaa tcgccaacgt gccgcaggct tgcgccatcg cttcgatgat
    27001 gagtacgccg ggcatcacgg gcaggtcggg aaagtggcct tggaactggg gctcgtttat
    27061 gctgacgttt ttaatcgcgg tcagggtttt catcggctcg aaggcggtga tgcggtcgag
    27121 ctggagaaac ggatagcggt gggggatgag tttttggatg tctttggctt cgatggggag
    27181 ttgtacgtcc atgtctgtcg tattccttga ataaaatcgg tttggattta ttgcgtgtct
    27241 ttgccgtctg aaagctgctg ttccagcgtt ttgaggcgtt tgttcatttc gcttaagcgg
    27301 tggatatgga cggcgttgcg cgcccattct ttatgggtgg acatcgggaa gatgccggcg
    27361 aggtgtttgc cgctttcggt aatgctgtgg gtgacggacg tgccgccgcc gatggtggtt
    27421 ttgtcggcga tttcgatgtg tccgaccgta ccgacgccgc cgccgatgat gcagtagctg
    27481 cctatggtta cgctgcctga gatgccggtt ttggcggcga tgacggtgtg cgaaccgatt
    27541 ttgcagttgt gtccgatttg gacttggttg tcgattttgg tgccgttgcc gacggtggtg
    27601 tcgctcatcg cgccgcggtc gatgttggtg ttcgagccga tttctacgtc gtcgcccagc
    27661 gttaccgcgc cggtttgcgg gattttaaac catgaatcgc cggcaaaagc gagtccgaaa
    27721 ccgtccgcgc cgatgaccgc gccgctgtgg atttcgacgc gtctgcccag tgtgcagccg
    27781 taataaacga cggcgttggg atgcaggacg acttcgtcgc ccagtttgca atcgtgttgg
    27841 acgacggcgt ttgccaagat gcggcagcct tcgccgagca cggtgtttgc gccgatgtag
    27901 acgttcgcgc cgatttcgca gctggcggga acggtcgcgc ccggttcgac gacggcggtc
    27961 ggatggatgc cgccgcgcgc tttgacgacg ggtgaaaaca ggcgggcgac tttggcgaaa
    28021 tagagatagg ggtcgtcggc gacaatcagg ttgcgccatt caaatccgtc tgccgctttg
    28081 gcggaaacga tgaccgcgcc cgcgctgctg tcgtggactt cggctttgta tttcggattg
    28141 gcaaggaagc tgatgtgttc cgcctgcgcg tctgcgagcg ggcgcacggc ggtaacggaa
    28201 atgtcctcgc cgcgccattc gccgccgagc cgcgcggtga tttgggacag ggtgtaggtg
    28261 gccggaatca tggttttcct gttcggtatg ccgtctgaaa gggtcagcgg gcgttcattt
    28321 ctttaatgac gctgtcagta acgtcgtatt gggtgttgac gtaaatcacg ttctgcaaaa
    28381 tgacatcgta accttcctgt ttggcgattt tgacgatgac gcggttggcg ttttgctgga
    28441 gggaggcaaa ctcttcgttg cggcggaggt tgtagtcttc ttcaaactgc gcctgttttt
    28501 tgcggaacgc tgcgaccagc ccgcgccatt tttcttcggc ttgcgccttt tttgcgtttc
    28561 tgagtttgcc ttcggcaagc tgcctttcca aatccagacc ttcgcgttgc agtttttgca
    28621 attcgtcctg acgggcggaa aattcgctgt ccagcgtttt ttgaatcttg cgcgcctgct
    28681 tggattcgag gtagatgcgc tcggtgttga taaagccgat tttttggaag gtgtcggcgt
    28741 gcgcgcctgc ggtgcagcac aaaccgatca gagccgcggc aaacgcgcgg gtcaaacggg
    28801 tcatggtaaa actccttcga atgttgccgc gaaatgccgt ctgaagggct tcagacggca
    28861 tttgcgggat tagaacgtcg tgccgagttg gaattggaag cgttggattt cgtcttccgg
    28921 ttttttcttc agcgggtagg cgtagctgaa tttcatcggg cctaaaggcg agagccaggt
    28981 aaccgcgccg ccggcggaat agcgcaattc gttggtaaag gtggatttat gggtattgcc
    29041 ggcgccgtaa atgttttgaa ccctgccgcc ggtcgcggaa ctgctgttgt cgtcgtaggt
    29101 tttgccgtcc cacacgctgc ctgcgtcggc aaacaggctc aggcggacgg tgcgcgcgtc
    29161 tttcgcgcca ggcatcggga agagcagctc ggcggagacg ttggcttttt tgttgccgcc
    29221 gtagctgatt ttttcgccgt attcgtcata cactttcgga ccgagcgtgc cgctttcgta
    29281 tccgcgcacc gaacccaggc cgccgccgta gaagttttca aagaagggga tttctttggt
    29341 tctgccgtag ccgcccgcaa tgccgacttc gccgccgagc atcagcgtga aggttttgct
    29401 taaggggaag aaccaggttt ggttgtgggt ggcggagtag tattgcagtt tgctgccggg
    29461 cagggcgatt tcggcgttca cgcccgtcag gtagccgcgc gtcggccata acgcgctgtc
    29521 ggttttgttg cgcccccagc cgacggtacc tttgtacagc cagcctttga agctgccgtc
    29581 tgtgccgtcg gttttgccgt atttcttgat aaagtcggca tagtgtttgg gcgctttgtt
    29641 gtaggtgttg acggtcaggt gttctgccac caaaccgaaa ttcacgcggt cgtattcggt
    29701 aacaggcacg ctcatgcgga tgcctgcgcc tgccgtggtg gttttatatt gtttgatgct
    29761 ggtcgatgct ttgcgcgggt cgaaggcttt tccgtaaaca tcgtagccca ggctgacccc
    29821 gtctgccgtg aagtacgggt cggtaaacga cagcgagccg ttgagcgtgg ttttgcttcg
    29881 tgaggcgcgc agggcggccg acttgcccgt accgaacagg ttgtcttggg aaacgcctgc
    29941 ggacatgacc aggccggtat cctgtaccca gcccgcgctc aagtcgagcg agccggtgga
    30001 acgttcggtc aggctcatgt tcaaatcgac tttgtcgggt gtgccggcaa gcgggacggc
    30061 gtcaaactgg acattgtcga agtagcccaa aagctcgacg cgctctttgg agcgttgcag
    30121 cttggaggtg tcgtaaggcg cggattccat ttggcgcaat tcgcggcgca cgacttcgtc
    30181 gcgagttttg ttgttgccgg tgatgtggat ttcgttgacg tagattttcc ggcccggttc
    30241 gatgtgcagg acgaaatcga cggttttggt ttcggcgttt ggcagcggct gtacgctgat
    30301 ttcgctgtat gcgtagcctg ccgagcccat gcggttctga atctcaccca aaacggcggt
    30361 catctgctgg cgttcgtacc atttgccggg cttcatggtc agcagctttt ccagttcggc
    30421 tttggggact tcgttggtgt cgccttcgat ggagactttg ccccaacgga aacgtccgcc
    30481 ttcgtggacg gtgattttga tggtctgctt ggttttgtct tcgttggttt ggatgtcggt
    30541 atcgaggatg cggaaatcga agtagccgtt gttctggtag aagtcggtta ctttttccat
    30601 gtcttgggca aatttctgct cgttgaattg gttgcttcgt gtcagccatg tccaaatgcc
    30661 gccttcggtc agcgacatct gccgcatcag tttgcggtcg gaatagactt ggttgccttc
    30721 aaattcgatg tcggtgattt tggcggattt gccctcgtca atcgtgatgt cgatgtcgac
    30781 gcggttgcgg gcgagtttgg ttactttggg cgtgatttgg atattgagtt tgccgcgccc
    30841 gaggtattct tctttcaggc cggcgactgc ctgattgagt gtcgcctgat taaagtattg
    30901 cgactgcgcc agcccgaacg attcgaggtt tttcttaatg gcatcgtttt gcagcatttt
    30961 tgcgccggtg atattgagcg atccgatggt ggggcgttcg atgacggtca gcaggagctg
    31021 cccgtccgca gtctcgacgc gtacgtcgtc aaagaaaccg gtggcgtaca ggcttttgat
    31081 gatggcactg ccgtgtgtgt cgttgtaggt gtcgccgatt ttgacgggca ggtagttgaa
    31141 tacggtgctt ggctcggtac gctgcaagcc ttcgacgcgg atgtcttgga tggtgaagtc
    31201 ggcaaatgcc aaaggcgata tgcccaacat catcagtgcg gaagcaatct gtttcagttt
    31261 catcgtaagt tccttgtggt gcggaatgcg gtttcagacg gcattccgaa acgtaaaatc
    31321 taaccgagca gccgggtaac gtcgttgaag aaggcgaccg ccatcatcag catcatgagg
    31381 gcaagcccga agcgcaaacc gatgttttgg acgcgttcgc ccaaaggttt gccgcgtatc
    31441 cattcggcag tataaaacac gaggtggccg ccgtccaaaa cggggacggg cagtaggttc
    31501 agcacgccga ggctgatgct gaccagtgcc aaaaattcca aataactttg caagccgagt
    31561 tcggcggact gtccggcaat gtcggcaatg gtcagcggcc cggaaatatg gctgacggag
    31621 gcgttgccgc tgattagttt gccgaaaaat ttgagggttg tccacgagtg ggaaacggtt
    31681 ttttcccagc ccatgccgaa tgcgcggata acagacggac ggtagctgcg gcggatttgc
    31741 gcgtcccacg ccctgtccgg ctgcggacgg aggccgacgc gcccgatcag ggtgtggtcg
    31801 gactgttcga cagtatcggg gcggatgtcg gcggtatggg tttgtccggc gcgttcgtag
    31861 gttaaggcga ttttcctgcc ggggctttgg cgggtcaggt ttgcccattc ctgccacgag
    31921 gcgatgggtt tgccgtcggc ggcagtcagc ctgtcgtccg gttgcagacc tgctttgtcg
    31981 gcgggactgc ctttttccac gccgccgaca acggttgtga ttttaaaggg catcagtccg
    32041 atgtagcctt ggttttttgc gattttaccg gcttccggcg tgcctgcggc atcgatggtg
    32101 cggacggttt gcgcgcccga tgccgtctga acgccgacgg cgactttgcc tgcctcaagg
    32161 ttgaggacga tttcggtttg cgcgctgccc caatctgcaa cgggtgtgcc gttgacggat
    32221 tgtattttgt cgccgctttg gaagccggcg cgggcggcaa tggtgtcagg ttcgactgtg
    32281 ccgatgtagg ggcgcagttc ggttacgccg aaggaaaagc tcagtccgta cagcaaaacc
    32341 gccagtgcga ggttggtcag tgggccggcg gcgacgatgg cgatgcgctt ggcggggtgt
    32401 tgtttgtcaa aagcgtaggg taaatcggct tctgatactt cgccttcgcg cgtgtcgacc
    32461 attttgacgt aaccgcccaa cggaatcggg gcgaggcacc attcggtgtc gccgcgcttt
    32521 cgggtgaaaa acggtttgcc gaagccgacg gaaaagcgta cgactttgac gccgcacaat
    32581 ctggcaacga tgtagtgtcc gaactcgtgc aggctgacca aaatcaggat ggcgaagata
    32641 aaagctagaa gggtgtgcaa atggttttcc tttgataacg gtgttcggac ggcatcagcg
    32701 cagtgtgccg ataaatgccc gcgcttgtgc gcgtgtccgg gcatcttgcg ccaagagccc
    32761 ccctatgtcg cctatgccgt ctgaaaagtc ttgtgaaaga caatgggcga cggttttggc
    32821 aatgtcggta aacttaatct gtccgtccaa aaaggcggcg acggcggctt cgttggcggc
    32881 gttcaatacg cagggcgcgg ctccgcctgc gttcatggct tcataggcga gcttcaggca
    32941 ggggaagcgg tcaaagtcgg gcttttggaa ggtcagcgcg gacaatgcgt cgaaatcaag
    33001 gtcgccgaca cccgaatcga tgcgctcggg caagcccaaa caataggcga tgggcgttcg
    33061 catatcggga ttgcccagtt gtgccaacac ggagccgtcg cggtagcgca ccatgctgtg
    33121 tatcacggat tgcggatgga tgacgacttc gagcttgtca ggcggacagt tgaacagcca
    33181 atgcgcttca atcagctcca aacctttgtt catcatggtg gcggaatcga cggagatttt
    33241 gcgccccatg ctccaattgg ggtgtttgac cgcttgggcg ggcgtaatgc tgtcgaacgt
    33301 gcctaaatcg gtcgttaaaa atgggccgcc ggaagcggtc aggataatcg aagcgatgcc
    33361 gtgttcgttc agacgacctg tgtaatcgcg cggcaaaact tggaaaacgg cgttgtgttc
    33421 gctgtcgacg ggtaacactg ccgcgctgtt tgcacgggcg gtttccataa acaacgcgcc
    33481 ggaaaccacc agcgtctctt tgttcgccag ataaatggtt ttgccttttt gcgccgctgc
    33541 gagcgcggaa ggcagcccca ccgccccgac gatggcgcac atgacaccgc tgacttcgtc
    33601 ggcagaggca acgtcaacca atgcctgcgc gccgtgtaaa acctgagtcg ccgtgccgtc
    33661 gcgtttcaac agggcttcaa gccgggcggc gtattcggca tcggcaacga cggcatattc
    33721 gggttggaac gtttgacatt gagccgccag tttctcgacc tgcttatgcc ctgccagcgc
    33781 gaatacgcgg aatttttcgg ggtggcggga gacaacgtcc agcgtgcttt cacctatgct
    33841 gccggtactg cctaatatgg tcaggacttg tggtgtcata atgaggataa ctttataccg
    33901 gatgccgtct gaagcgtttt cagacggcat agaatcaatt taaaaccgac atcatcgctg
    33961 catagacgct gataacggca atcaggctgt cggtacggtc gaacacgccg ccgtgtccgg
    34021 gcagcagctt gctgctgtct ttgatgcctg ccgcgcgctt gagccagctt tccaaaaggt
    34081 cgccgcatac gctgacaacg gtcagcacca aaccgattaa cacggtatcg aaccagcctg
    34141 tatcgaatgc cagccagccg gcacttcgta cggcggtcat gtacactgcc acgcaaaccg
    34201 cgccgccgat tgcaccttcc cagcttttgc cggggctgat tgccggcgcg attttgtgtt
    34261 tgccgaacgc cttgccgctg aaatacgcgc aaatatcggc aacccacacc aaacccatca
    34321 cggcgagcag cggcagggca tcatcgggat gcgggcgcag ggatacgagc gcgaaccaaa
    34381 acggcatgac cagaagccag ccgacggcat aaacctgcca accgccgttg agcctccatt
    34441 tgaatctcaa ccataaaggc ataacggcga gccaaaatgc caaaacaaca taccaaacca
    34501 aattaggcag catccagccg cccgcatagg caaccacgcc gaaaaccaag gttgcggcga
    34561 ggtaatggtt ggttttaatt ttgcacaaac cgcccatacg ggcatattcc cacaaggcaa
    34621 tcagggcaat cagtccgcaa aatgcagccc acaaccattg cggcgcgtaa aacagcatgc
    34681 ccagcatcag cggcagcagc cacatggcgg ttattacccg ttgtttcagc atattcagtt
    34741 cctttgctgt tcgataggca gttgctcgga ggtgcgtccg aaccgccgtt cgcgtttttg
    34801 gaacgaagcg acggcatcgt ccaaagcctt gccgtcaaaa tcgggccaca aaatatcggt
    34861 gaaatacagt tctgcatatg ccatctgcca gagcaggaaa ttgctgatgc gcgtttcgcc
    34921 gccggtgcgg atgaacaaat ccggttccgg tgcatcgccc agcatcaagt gtttcgccag
    34981 cgtgtcttcc gtaatctcgg atacgccttc ggcaatcagt ttgtttgccg cctgcaaaat
    35041 atcccagcgg ccgccgtaat cggcggcaat gctcagggtc aggccggtat tgtttgccgt
    35101 caacgcttcc gcctcttcga tgccttgcag aatctgccgg ttgaagcgtt cgcggctgcc
    35161 caatatcttc aggcgcatat tgttttcgtg caggcggcgt acctgttttt gcaaagcctg
    35221 taaaaacagc cccatcagga acgaaacttc gtcttcgggg cggcgccagt tttcggttga
    35281 aaaggcaaac acggtcagat attgcacacc cagtttggcg caatgcttca ccatattttc
    35341 caatgcgtcc aaaccgcgtt tgtgtcccat tatgcgcggg aggaaacgtt ttttcgccca
    35401 acggccgttg ccgtccataa tcacggcgat atgcttggga atggcggtgt gttccaaaac
    35461 ggcctgcgtg ctgcttttca tgtctgcctt tcgcggttcg gcattcaaat gccgtctgaa
    35521 cgccgaaccg tgcaggttaa attgccatca aatcttcttc tttggcagtc aggagtttgt
    35581 cggcttcggt aatgtatttg tcggtcagtt tttgaaccgc ttcttcgccg cgacgtgcct
    35641 cgtcttcgga aatttctttg tctttgagga gttttttgat gtggtcgttg gcatcgcggc
    35701 gcacgttgcg gatagagacg cggccttctt ccgcttcgcc gcgtacgact ttaatcaggt
    35761 ctttgcggcg ttcctcggtc agcatgggca tcggcacgcg gatcaggtcg ccgacagctg
    35821 ccgggttcag tcccaagttt gaatcgcgga tggctttctc gactttggcc gccatattgc
    35881 cctcaaacgg tttcacgccg atggtgcgcg cgtccagaag cgttacgttg gcaacttggc
    35941 tgacggggac catgctgccc cagtattcga cttccacttg gtcgagcagg ccggtatgcg
    36001 cgcggccggt acgcactttc gccagatttt ctttcagtac ttcgaccgaa cgctgcatct
    36061 tgccttcggc tgttttttga atatcgttga tcatattgtt ctttcggtgg gataaggtgg
    36121 gcgggagacc gtctgaacgc gtttcaagcc gttcagacgg cataaagacc gttaaccgcg
    36181 aatagtaccg ttattcgggc ataacgacaa ggtaggcgga ttggggatgc cgtctgaagc
    36241 gacaggcgtt tcagacggca tcgtgtccga ccgtcagccg tgttcccgtg tttcaagcag
    36301 gctttggcgc aggtgttggc gttcgtgggc atccagccat ttgcggcggg tgcgttgcag
    36361 caggatgacg agggcggaaa tttcctgacg catattggtg ctgagccaga ggaagccctg
    36421 ccattggtag tggaggtgtt cggcgagggc ttccagttcg gggttgatgg cggtgtcgat
    36481 gcggatgcgg cgggcgtgtc tgccgttgat aagggcgacg gtttgttgca ggtcggtttg
    36541 gagcagtgtg aagtggcggt caagcagccg gatttcgctg ccgttgagtt tgggagattg
    36601 cagcttggcg gcggtggtca ggagcagctc ggtggtgttg acgattttac ggtgggcgtg
    36661 ctgcatggct tccatcatgg cggggctgat gcggctttcg cccgatgtgg cggcgagatg
    36721 gctgcggctt ttgaccatgc gtgcgttgat ttggcgcatt ttcgccatgt tctcctcgag
    36781 gcgttcgcgg gtcatgcgcc tgccgttgct gatttcggca atcattttgc tgcagtcggc
    36841 caggttgtcg gcaagcatga aacgccacat cagtgtggat ttcagcggca gcagtttggc
    36901 ggcggcgatg gcgatggccg cgccgatgag gacgttcatg gcgcgcatga gtccgctgtc
    36961 gagccattcg ctgccgttgt cgccgatgag catacacatc gtcagccctg ccagcatagg
    37021 gacgtagccg tttttgccga ccgccgccca gccggccagt gcgcttgccg tgccgacggt
    37081 gaggtagaag aggaggttgc cgtggaaata atgctggttc agccataaaa cgcccaaacc
    37141 cgcgcccagc ccgatgaccg tgccgagcat acgttccacc gccttggagt aaatcgcccc
    37201 ttgaaactgg agcatgccga ggacgacgaa gacggtcatc cctatccact cgccgtgttg
    37261 gaggtggagc agccgggcgg aggcggtggc gaacaggacg gccccgccga gccggacgga
    37321 atggatgagg cggcggtagc ggtagcgttc gtaggagttg agccagcggc tgacgaggcg
    37381 gttgcgttgc gaggtgttca tatcggttgt gccgtctgaa gcggaaatgt gaaaaagcac
    37441 aggcttcccg aggaagggag ggtctgtgct tggtattggt gccggagaag ggaatcgaac
    37501 ccccgacctt cgcgttacga atgcgctgct ctaccgactg agctacaccg gcgttttttc
    37561 gtcatgatat atatgaacgg ttgtttgtgc aacttttcgg gcgggcggca aggcagtgc
//
LOCUS       DAWHRS010000003        36171 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWHRS010000003 DAWHRS010000000
VERSION     DAWHRS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36171
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.003"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..239)
                     /locus_tag="WIB82_000091"
     CDS             complement(<1..239)
                     /locus_tag="WIB82_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEZ3841469.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLR"
     gene            complement(226..1155)
                     /locus_tag="WIB82_000092"
     CDS             complement(226..1155)
                     /locus_tag="WIB82_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ3841470.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            complement(1152..1799)
                     /gene="pxpB"
                     /locus_tag="WIB82_000093"
     CDS             complement(1152..1799)
                     /gene="pxpB"
                     /locus_tag="WIB82_000093"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ3841471.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            1983..4190
                     /gene="uvrD"
                     /locus_tag="WIB82_000094"
     CDS             1983..4190
                     /gene="uvrD"
                     /locus_tag="WIB82_000094"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ3841472.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     NAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTIENLQTASNEQGITLWQAACNAGAK
                     ATKVAAFVRLIEALRNQVGQMPLPEIIVGILKDSGLTEHYKTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGADEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            4547..5086
                     /locus_tag="WIB82_000095"
     CDS             4547..5086
                     /locus_tag="WIB82_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ3841473.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(5276..5491)
                     /locus_tag="WIB82_000096"
     CDS             complement(5276..5491)
                     /locus_tag="WIB82_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841474.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(5499..5702)
                     /locus_tag="WIB82_000097"
     CDS             complement(5499..5702)
                     /locus_tag="WIB82_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841475.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(5615..5974)
                     /locus_tag="WIB82_000098"
     CDS             complement(5615..5974)
                     /locus_tag="WIB82_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ3841476.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            6027..6374
                     /locus_tag="WIB82_000099"
     CDS             6027..6374
                     /locus_tag="WIB82_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3841477.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVLVPF"
     gene            6602..7222
                     /locus_tag="WIB82_000100"
     CDS             6602..7222
                     /locus_tag="WIB82_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ3841478.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            7245..8696
                     /locus_tag="WIB82_000101"
     CDS             7245..8696
                     /locus_tag="WIB82_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ3841479.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     IGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            9158..9514
                     /locus_tag="WIB82_000102"
     CDS             9158..9514
                     /locus_tag="WIB82_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ3841480.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGSKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKELGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            9505..9987
                     /locus_tag="WIB82_000103"
     CDS             9505..9987
                     /locus_tag="WIB82_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ3841481.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            10000..10593
                     /locus_tag="WIB82_000104"
     CDS             10000..10593
                     /locus_tag="WIB82_000104"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ3841482.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYVSVMTT
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVQNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            10583..11839
                     /gene="nuoD"
                     /locus_tag="WIB82_000105"
     CDS             10583..11839
                     /gene="nuoD"
                     /locus_tag="WIB82_000105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ3841483.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVTPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            11839..12312
                     /gene="nuoE"
                     /locus_tag="WIB82_000106"
     CDS             11839..12312
                     /gene="nuoE"
                     /locus_tag="WIB82_000106"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ3841484.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFIADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            12702..14003
                     /gene="nuoF"
                     /locus_tag="WIB82_000107"
     CDS             12702..14003
                     /gene="nuoF"
                     /locus_tag="WIB82_000107"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ3841485.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            14003..14440
                     /locus_tag="WIB82_000108"
     CDS             14003..14440
                     /locus_tag="WIB82_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841486.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRGNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            14730..16991
                     /locus_tag="WIB82_000109"
     CDS             14730..16991
                     /locus_tag="WIB82_000109"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ3841487.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFSYEGLYHESRLKK
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     TEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLSSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            16994..18070
                     /gene="nuoH"
                     /locus_tag="WIB82_000110"
     CDS             16994..18070
                     /gene="nuoH"
                     /locus_tag="WIB82_000110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ3841488.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            18151..18630
                     /gene="nuoI"
                     /locus_tag="WIB82_000111"
     CDS             18151..18630
                     /gene="nuoI"
                     /locus_tag="WIB82_000111"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ3841489.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            18661..19368
                     /locus_tag="WIB82_000112"
     CDS             18661..19368
                     /locus_tag="WIB82_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841490.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            19404..20075
                     /locus_tag="WIB82_000113"
     CDS             19404..20075
                     /locus_tag="WIB82_000113"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ3841491.1"
                     /translation="MTFQLILFYIFAVIILYGALKTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPTDYNNIRDLGSRIYTEYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGFKPE
                     GEGKA"
     gene            20072..20377
                     /gene="nuoK"
                     /locus_tag="WIB82_000114"
     CDS             20072..20377
                     /gene="nuoK"
                     /locus_tag="WIB82_000114"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ3841492.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDGLKG"
     gene            20411..20986
                     /locus_tag="WIB82_000115"
     CDS             20411..20986
                     /locus_tag="WIB82_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ3841493.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            21110..21451
                     /locus_tag="WIB82_000116"
     CDS             21110..21451
                     /locus_tag="WIB82_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841494.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            21500..23524
                     /gene="nuoL"
                     /locus_tag="WIB82_000117"
     CDS             21500..23524
                     /gene="nuoL"
                     /locus_tag="WIB82_000117"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ3841495.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIVSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYLAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPVYVLFENKYYLDVLYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            23620..25116
                     /locus_tag="WIB82_000118"
     CDS             23620..25116
                     /locus_tag="WIB82_000118"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ3841496.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGSDSRAPFARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLIMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIEQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     QDINCREFAILAILAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            25126..26568
                     /gene="nuoN"
                     /locus_tag="WIB82_000119"
     CDS             25126..26568
                     /gene="nuoN"
                     /locus_tag="WIB82_000119"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ3841497.1"
                     /translation="MNWSDLNLMPAMPEIVLLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFTFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMFAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGHVWLSVFAVIMSLIGAFYYLRVVKVIYFDVPDHDQPVGSNYAAKFV
                     LTVNAFLLLLWGIMPQTVIDWCAKALENTL"
     gene            26680..26979
                     /locus_tag="WIB82_000120"
     CDS             26680..26979
                     /locus_tag="WIB82_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ3841498.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAVLAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(27088..27984)
                     /locus_tag="WIB82_000121"
     CDS             complement(27088..27984)
                     /locus_tag="WIB82_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ3841499.1"
                     /translation="MNPTNDLKTWQQRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(27968..28192)
                     /locus_tag="WIB82_000122"
     CDS             complement(27968..28192)
                     /locus_tag="WIB82_000122"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ3841500.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDTDGLKELNLESDE"
     gene            complement(28439..29362)
                     /gene="rsgA"
                     /locus_tag="WIB82_000123"
     CDS             complement(28439..29362)
                     /gene="rsgA"
                     /locus_tag="WIB82_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ3841501.1"
                     /translation="MPSEHPFSDGIPTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(29367..31223)
                     /locus_tag="WIB82_000124"
     CDS             complement(29367..31223)
                     /locus_tag="WIB82_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3841502.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(31249..31386)
                     /locus_tag="WIB82_000125"
     CDS             complement(31249..31386)
                     /locus_tag="WIB82_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841503.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(31386..31970)
                     /gene="ruvA"
                     /locus_tag="WIB82_000126"
     CDS             complement(31386..31970)
                     /gene="ruvA"
                     /locus_tag="WIB82_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ3841504.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(32052..32378)
                     /locus_tag="WIB82_000127"
     CDS             complement(32052..32378)
                     /locus_tag="WIB82_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ3841505.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(32440..33159)
                     /locus_tag="WIB82_000128"
     CDS             complement(32440..33159)
                     /locus_tag="WIB82_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ3841506.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQAHGMVRAVLTAG"
     gene            complement(33156..33329)
                     /locus_tag="WIB82_000129"
     CDS             complement(33156..33329)
                     /locus_tag="WIB82_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ3841507.1"
                     /translation="MKRGGNTVTQAAFERRFMPSENLSGHIPNRRPHCTAVRNVSGGH
                     DAHSSSTQERNTF"
     gene            complement(33289..33753)
                     /gene="trmL"
                     /locus_tag="WIB82_000130"
     CDS             complement(33289..33753)
                     /gene="trmL"
                     /locus_tag="WIB82_000130"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ3841508.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(33818..34315)
                     /locus_tag="WIB82_000131"
     CDS             complement(33818..34315)
                     /locus_tag="WIB82_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ3841509.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKLGANRICCWTLAR
                     TPMKK"
     gene            34371..35285
                     /gene="bioH"
                     /locus_tag="WIB82_000132"
     CDS             34371..35285
                     /gene="bioH"
                     /locus_tag="WIB82_000132"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ3841510.1"
                     /translation="MCLKHRGQLFAASVRYAPKSAAQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKFMPDAAKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLTAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADRIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            35287..36075
                     /locus_tag="WIB82_000133"
     CDS             35287..36075
                     /locus_tag="WIB82_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3841511.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKCRLKENGIESRSA
                     LFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         8239 a         9181 c         9665 g         9086 t
ORIGIN      
        1 cgcaaatcgg cttcggacag attcatttcg gtacggccga agttttcccg atcgggatag
       61 ccggggtgtg ctccgatgcg cacgccgttt tgttgggcat acgccaatgc cgcccgaata
      121 tcggcaatgc tgccggcgtg ttgggcgcag gcgatgttgg ccgaagtaat cagctgcaac
      181 aaggcttcgt cgctgccgca gccttcggcg agatcggcgt ttaaatcaac ctgcttcatg
      241 ggtgattctc cgtatttggt tcagatagac ttggtttttg cgccgcaggg cggtggcttc
      301 tttcaagccg attattttga atttgacttt gctgccgaag cgcacctgtg ccagcctgcc
      361 caaatcggcg gcggcaacgg tagcgatttt cggataaccg ccggtggttt gcgcatcggc
      421 cagcaggata atcggtttgc cgccgggcgg cacctgcacg gttcctgcct gaacagcgtg
      481 ggacagcatt tccaaaggtt gcgacagggt cagcggctgt ccgtcgaagc ggtagcccat
      541 gcggttgcta tcgctttgca gcgtccacgt ttcccgttcc agattcagac gccctttttc
      601 actgaaagcg gcatattccg acgaaggaac aaggtggacg gtatcggtaa acggtatcgg
      661 ggcaatgccg actttggaca attcctgcgc acctttgccg atggggagac aatcaccttt
      721 ttgcagcatt ctgccctgat ggccgccgaa accggctttc aggtcggtgc ttctcgaacc
      781 catcacttcc ggcacatcaa atccgcccgc cacgcacaca tagccgtaca tgccctgcac
      841 ggcacgcacc attttcaagg tctgcccttt gcgggcggta taacgccaat acgaatagac
      901 cggttcgccg tccaattccg cctgatacac ggcaccggtg agacaaaacg gcgtatcccg
      961 ttcaaacacc agcattatcc cgcccaaagc gatttcgatt gcggccgtgc cttcgtcgtt
     1021 gcccaataaa atattacccg ccgccaaagc aaccgtgtcc atcgcaccgg catgaccgat
     1081 gccgtaacgc cggtgtccgt agcgtccggt atcctgaata tgcgccggtg cctgcactgc
     1141 cgaaacgtga atcatggctc aatcctttct gcaacaaagc ggacttggtc acccgccgcc
     1201 agcagggtcg gcggattcaa atcggctcgg aacaagggta attcggttct gccgataatc
     1261 tgccagccgc cgggcgaagc gaacggatac acaccggtct gactgccgcc gataccgacc
     1321 gaaccggcag gaacggacgt tctcggcacg gcacggcggg gcgtgtgcaa tgcttcgggc
     1381 aagccgccca gataagggaa accgggctgg aagcccatca taaatacggt ataagtttgc
     1441 gccgtatggc ggcggacgat ttcggaaata accgtctgat ggaaagcagc gacttccgcc
     1501 aaatccgggc cgtattcgcc gccgtagcag acgggaattt ccaccagttt gccctgatgg
     1561 tctgtaacgg cggtgtgttc ccacacatat tgcaattcat cggcaagcgt cgccaaatcg
     1621 gtatcgaaac gggtgaacac ggtcagattg ttcatgccga ccaccacttc ctcaatcctg
     1681 tcgtgctgcc cgagcgcagc ggcaaacgcc cacaactttt gctgtttgcc cagttcggaa
     1741 ggtgcattca gtcggcagac caaagcggat tcgctgattg gtgtgatctc tattctcatt
     1801 tgttgttcat tttggttatg ttttaatgaa tctatatgca ggggcggcgg tttgtcaata
     1861 tcttctgtgc tgcatcatca aaccgtcgat tggaaaagtg ctgccctgcc gctgcacttt
     1921 ttcagacgac cttaaaccgt ttctattaaa atagcgcatt ccacttttca gacggcatct
     1981 ctatgtttcc cgaccaatcc gcccccaacc tgctgcaagg cttgaatccc gaacaacttt
     2041 ccgccgtaac ctggccgccg caatccgccc ttgtgctggc aggcgcgggc agcggcaaaa
     2101 cgcgcgtgct gaccacgcgt atcgcatggc ttttgcaaac cggtcaggca agcgtgcaca
     2161 gcattatggc ggtaacgttt accaacaaag ccgccaaaga aatgcaaacc cgactcggcg
     2221 cgatgattcc cgtcgacgtc cgcgctatgt ggctcggcac gttccacggc ctctgccacc
     2281 gctttctacg gctgcaccac cgcgatgccg gtttgccgtc ttcctttcaa atcctcgacg
     2341 gcggcgacca gctttccctc atcaaacgcc tgctcaaaag cctcaacatc gccgaagaaa
     2401 tcatcgcgcc gcgttcgctg caaggcttta tcaacgcgca aaaagaatcc ggtttgcgcg
     2461 cttccgtctt gagtgcgccc gatccgcaca caagccgcat gattgaatgc tacgccgaat
     2521 acgacaaaat ctgccaacgc gaaggcgtgg tcgattttgc cgaactcatg ctccgcagct
     2581 acgaaatgct gcaaagcaac gaaatcctgc gccagcatta ccaaaaccgc ttcaaccaca
     2641 ttctcgtcga cgagttccaa gacaccaaca aactgcaata cgcctggctc aaactcatgg
     2701 cgggcggcaa cgcggcagtg tttgccgtcg gcgacgacga ccaaagcatc taccgcttcc
     2761 gtggcgcaaa cgtcggcaat atgaccgcgc tgatggaaga gttccatatc aacgcgcccg
     2821 tcaaactcga acaaaactac cgctctgtcg gcaacatcct tgccgccgcc aatgccgtta
     2881 tcgaaaacaa cgacgaacga ctcggcaaaa acctgcgcac cgacgccgaa gcaggcgaca
     2941 aaattcgcta ctactccgcc tttaccgacc tcgaagaagc ccggttcatc gtggacgaaa
     3001 ccaaagccct cgaacgcgaa ggctgggatt tggacgaaat cgccgtcctc tatcgcagca
     3061 acgcccaatc ccgcgttatc gaacaaagcc tgttccgcag cggcatccct tacaaaatct
     3121 acggcggcct gcgcttttat gaacgccaag aaatcaaaca cgcgctcgcc taccttcgcc
     3181 tcgccgttaa tcccgacgac gacaacgccc tcttgcgcgt catcaacttc ccgccgcgcg
     3241 gcatcggcgc acgcaccatc gaaaaccttc agacggcctc aaacgaacaa ggcatcaccc
     3301 tttggcaggc agcctgcaac gccggcgcaa aagccaccaa agtcgccgcc ttcgtccgcc
     3361 tcatcgaagc cctgcgcaac caagtcggac aaatgcccct gcccgaaatc atcgtcggca
     3421 tcctcaaaga cagcggcctg accgaacact acaaaaccca aaaaggtgac aaccaagacc
     3481 gcctcgacaa cctcgacgaa ctcgtcaacg ccgccatcga gttcaaaccc gaagacagca
     3541 actttgaaat cctgcctgaa aacatttcag acgaccccgc ctttcccatc ctcgccttcc
     3601 taagcaacgc cgccctcgaa tccggcgaaa accaagcagg cgcggacgaa aaagccgtcc
     3661 aactcatgac cgtccacgcc gccaaaggct tggaatttaa cgccgtcttc ctcaccggca
     3721 tggaagaagg ccgcttcccc agcgaaatga gtctcgccga acgcggcggc ctcgaagaag
     3781 aacgccgcct catgtacgtc gccatcaccc gcgcccgcaa acgcctctac atcaccatgg
     3841 cgcaacaacg catgctgcac ggacaaaccc aattcggcat cgtctcccgc ttcgtcgaag
     3901 agatcccacc cgaagtattg cactacctgt ccgtcaaaaa gaccgcctac gacagctacg
     3961 gcagcccgcg ccaaaccgcc gcatccaaag acaaaatcat cgacgactac aaacagcccc
     4021 aaacctacgc aggtttccgt atcggacaaa acgtccgcca cgccaaattc ggcaccggcg
     4081 tgattatcga tgccgcagat aaaggcgaat ccgcccgact gaccatcaac ttcggcaaac
     4141 agggcgtgaa agagttggat accaagtttg cgaaattgga agcgatgtaa atttgaaatg
     4201 caggctggat actcgtatcc gacaaaaata tttgacgcgt ctatcgtttc cgaaaaaccg
     4261 ctgttggaaa atgtcggatt cgagaatcag acctatggca aaaaactgta gcaaggacaa
     4321 ggcaaaaggc cgtctgaatc cggtaacaac aaattttgcc gaaacctccg ccgtcattcc
     4381 cgcgcaggcg ggaatccaga cctttcggca cgggaactta tcggataaaa ggtttcttta
     4441 gattccacgt tctagattcc cgcctgcgcg ggaatgacgg acggtaaaag ccgggtatag
     4501 atacctaacc cttgtttata gctgaaaatc aataaggaag aacattatgt cccaagtttt
     4561 taaagatttt gatttgtcct ccgtatggga atccgacagt tgggcagatg aaaactacaa
     4621 agaagccccg tttacccctg aaattttggc tgccgtagaa agtgaactgg gctataaatt
     4681 gccgcaaagt tttattgaat tgatggcggt acaaaacggc ggaatatttg tcaaaaactg
     4741 ttttccgacc acacagagaa attcgtgggc gaaagatcat gtgcaaattt gcgaggtatc
     4801 gggcatcggc tttgaaaaag aagggagttt gtgcggcgaa acagggcaaa aactttggat
     4861 ggaagaatgg gaatatccgc ccatcggcgt gtattttgcc aacgacccgt caggcggtca
     4921 tgccatgttt gccttggact atcgggagtg tggaaaagac ggcgagccga aagtggtgtt
     4981 tgtcgagcaa gaatcggatt ttgaaatcgt cgaacttgcc cccgattttg aaacctttat
     5041 ccgcagcttg cggcatgaag atgagtttat tgacgaggaa atataaaacg gcggttgaaa
     5101 aaccggaatc atcaagggaa aacgggcgaa ataacggata atcgcttgaa tccgtaagga
     5161 aaacggtttg gtggaacgcg ccatccaaga cctttgcaaa gaaaaactgt ctccgacagc
     5221 attgacatta ttaacagaac ttatcaagtt tggagctatc tcaaatataa ttcggttatc
     5281 cttttgtatc cattaaatca tatgcttcaa ttaattgttg ttctagctct tgtaccaatt
     5341 ttgaattgcg aattcctgac acaatctcaa attcttctgc atctatgcaa acacctgcat
     5401 aaatttcaat aacaagggaa cgcaataatt gaagctcttc tcttgttaaa gaaataataa
     5461 tgtcatcacc tgtgtaattg attatattca taataatttt atttttgttt gtcaaagtaa
     5521 gttttgccta aggttggtct aaatgcagtt ccaccatctt ttgaatttgg gtctctgatt
     5581 acaattgctc cagacttatc atcccaaatt gctcttatgt gtttggattg taatcttcga
     5641 attcccaaga aaaaaatcgt aataagtttg aaagtgtcaa atcccaagtt tcttttgagc
     5701 aatattctaa tattttatca atttcacttt taataatctt atgatcaaac tgttctaata
     5761 ttaatgcatt agaccaaaaa aaaccttctt tattacaatg atgggaaatc catttaggag
     5821 aacaaatgca aagtgaaaaa atagatgagc cttgttctcc ttcgattccg atatccaaat
     5881 ctatccatct atggaaatta tctggaattt cgggggtaaa tttttcaaaa tcaatatcat
     5941 ataaatttat gctttttaaa tccaatttaa tcattagggc tgtcctagat aaatagggaa
     6001 attcaaatta agttagaatt atccctatga gaaaaagtcg tctaagccgg tataaacaaa
     6061 ataaactcat tgagctattt gtcgcaggtg taactgcaag aacagcaaca gagcctgaca
     6121 gcattgttta tacggattgt tatcgtagct atgatgtatt agatgtgagc gaatttagcc
     6181 atttccgtat caatcacagc acacattttg ctgaacgaca aaaccatatc aatggaattg
     6241 agaatttttg gaaccgggca aaacgtcatt tacgcaagtt taacggcatt cccaaagcgc
     6301 attttgagct gtatttaaag gagtgcgaat ggcgttttaa caacagtgag ataaaagttc
     6361 ttgttccatt ttaaaacaat tagtaaaatc gagtttatct tagttgtcca ggacagcccc
     6421 attattttta taacaccgtg aagccgcaca gcagtttgaa cagtgatacg ccgtttgcgg
     6481 gcttacgagt ttattttccc ggcctgcagt ttgagcaata cggtgatttc ctacggttaa
     6541 tacaaatgtt tacacattga tacatttcat ttatagttcc gcctatttga aaatagaaaa
     6601 tatgaattcg accgcaagta aaaccctgaa aggattgtcg ctggtgtttt tcgcctctgg
     6661 attctgcgcc ctgatttacc aggtcagctg gcagaggctt ctattcagtc acataggtat
     6721 cgatttgagt tcgattactg tcattatttc tgtatttatg gtcggcttgg gtgtaggtgc
     6781 gtatttcggt ggacgcattg ctgaccgttt tccttcaagt atcatccccc tgttttgcat
     6841 cgctgaagta tccatcggtc tgttcggttt ggtaagcagg ggtctgattt ccggcttggg
     6901 gcatctttta gttgaggctg atttgcccat catcgctgct gccaatttcc tcttattgct
     6961 gcttcctacc tttatgatgg gcgcgacctt gcccttgctg acctgttttt ttaaccggaa
     7021 aatacataat gttggcgagt ctatcggtac cttatatttt ttcaacactt tgggtgcggc
     7081 actcggatcg cttgccgccg ccgaattttt ctacgtcttt tttaccctct cccaaaccat
     7141 tgcgctgaca gcctgcttta accttctgat tgctgcttca gtatggctgc gttacagaaa
     7201 ggatggatat agtgaacact aaaccgaata ctagtttaat ttatatgctt tctttcctta
     7261 gcggcttatt gagcttgggt atagaagtct tgtgggtaag gatgttttcg ttcgcagcac
     7321 agtccgtgcc tcaggcattt tcatttactc ttgcctgttt tctgaccggt atcgccatcg
     7381 gcgcgtattt tggcaaacgg atttgccgca gccgctttgt tgatattccc tttatcgggc
     7441 agtgcttctt gtgggcgggt attgccgact ttttgatttt gggtgctgcg tggttgttga
     7501 cgggtttttc cggcttcgtc caccacgccg gtatcttcat taccctgtct gccgtcgtca
     7561 gagggttgat tttcccgctc gtacaccatg tgggtacgga tggcaacaaa tccggacgac
     7621 aggtttccaa tgtttatttc gccaacgttg ccggcagtgc attgggtccg gtccttatcg
     7681 gctttgtgat acttgatttc ttgtccaccc aacagattta cctgctcatc tgtttgattt
     7741 ctgctgctgt ccctttgttt tgtacactgt tccaaaaaag tctccgactg aatgcagtgt
     7801 cggtagcagt ttccctaatg ttcggcatcc tcatgttcct actgccggat tctgtctttc
     7861 aaaatattgc tgaccgtccg gataggctga ttgaaaacaa acacggcatt gttgcggttt
     7921 accatagaga tggtgataag gttgtttatg gggcgaatgt atacgacggc gcatacaata
     7981 ccgatgtatt caatagtgtc aacggcatcg aacgtgccta tctgctaccc tccctgaagt
     8041 ctggcatacg ccgcattttc gtcgttggat tgagtacagg ttcgtgggcg cgcgtcttgt
     8101 ctgccattcc ggaaatgcag tcgatgattg ttgcggaaat caatccggca taccgtagcc
     8161 ttatcgcgga cgagccgcaa atcgccccgc ttttgcagga caaacgtgtt gaaattgtat
     8221 tggatgacgg taggaaatgg ctgcgtcgcc atcctgatga aaaattcgac ctgattttga
     8281 tgaatacgac ttggtactgg cgtgcctatt ccaccaacct gttgagtgcg gaatttttaa
     8341 aacaggtgca aagccacctt accccggatg gtattgtaat gtttaatacc acgcacagcc
     8401 cgcatgcttt tgctaccgcc gtacacagta ttccctatgc ataccgctat gggcatatgg
     8461 tagtcggctc ggcaaccccg gtagttttcc ctaataaaga actgctcaag caacgtctct
     8521 cccggttgat ttggccggaa agcggcaggc acgtatttga cagcagcacc gtggatgctg
     8581 cagcacaaaa ggttgtctct cgtatgctga ttcagatgac ggaaccttcg gctggggcgg
     8641 aagtcattac cgacgataat atgattgtag aatacaaata cggcagaggg atttaaccgt
     8701 cttaaagggt ttcaggcaac gcaggtttta ggtaacgtcc tgctagttca aaaaaaccgc
     8761 atcacagcag tcgggacaaa atggtttaaa cattttgtcc cgaattctta ttcctatata
     8821 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg
     8881 aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg
     8941 caacgccgta ctggtttttg ttaatccact ataccacgaa ttacggtgta aaaatttata
     9001 tgaccttata aaatcaaata agaatcgtta tcataacatg attgtattta ttgggttttt
     9061 ttgggcgttt tgccgatatt taccttttaa tggtttttga aattcgctaa aatacgaaat
     9121 tattgtagaa attttgttaa cggatttggg tgtaaccatg ttgtccgctt actttcccgt
     9181 ctttgtcttt atcctcatcg gcctcgcggc cggcgtactg tttatcctgc tcggcacaat
     9241 tttgggctcg aaacgtcact atgccgaaaa agacgcacct tacgaatgcg gttttgaagc
     9301 ctttgaaaac gcaaggatga agttcgacgt gcgctattac ctcgtcgcca tcctctttat
     9361 cttgtttgat ttggaggtcg cgttcatgtt gccgtgggca gtcgtgttca aagaattggg
     9421 cgcatacggc ttctggtcta tgctggtgtt tatcgtcgtt ttgacggtag gctttgttta
     9481 cgaatggaaa aaaggtgcgc tggaatggga atagaaggcg ttttgaaaaa aggtttcatc
     9541 accaccagcg cggatacggt gctgaactat atgcgtaccg gttcattgtg gccggttact
     9601 ttcggcttgg cctgctgcgc cgtggaaatg atgcatgcag gtatggcgcg ttatgacctt
     9661 gaccgtttcg gcatcatttt ccgtccgtcc ccacgtcagg ccgacctgat gattgtggca
     9721 ggtacgctga ccaacaaaat ggcgccagcc ctgcgccgcg tgtacgacca gctcgccgag
     9781 ccgcgctggg tattgtctat gggctcgtgt gccaacggcg gcggctatta tcactattct
     9841 tattccgttg tgcgcggtgc tgaccgcgtc gtgccggtag acgtttatgt gccgggttgt
     9901 ccgccgactg cggaagccct gatttacggc ctgattcagc tccaacaaaa aatcaagcgc
     9961 acttccacca tcgcgcgtga cgagtaagga gaggacgata tggcaagcat tcaaaactta
    10021 tacgagaccg tcgtcggcgt gcttggcgat caggcaagca aagtcatttc agctttgggc
    10081 gagattaccg tcgagtgtct gcctgaacac tatgtttcag tgatgactac gctgcgcgac
    10141 catgaagaac tgcatttcga gcttctggtc gacttgtgcg gtgtcgatta cagcacttac
    10201 aaaaacgaag tatggcaggg caaacgcttt gccgtcgtca gtcagctgct ttctgttcaa
    10261 aacaatcaac gcatccgcgt acgcgtctgg gtttcagacg acgacttccc cgtagtcgaa
    10321 tccgtcgtcg atatttacaa cagcgcggat tggtacgaac gcgaagcctt cgatatgtac
    10381 ggcattatgt tcaacaacca cccggacttg cgccgcatcc tgaccgatta cggctttgtc
    10441 ggacatccgt tccgcaaaga cttcccgatt tccggttatg tggaaatgcg ttacgacgaa
    10501 gagcaaaaac gcgtgattta ccaacctgtt accattgagc cgcgcgaaat cacgccgcgt
    10561 atcgtccgtg aggagaacta cggtggccaa taaattaaga aactacacca tcaacttcgg
    10621 cccgcaacac cctgcggcgc acggcgtatt gcgtatgatt ttggagttgg agggcgaaac
    10681 catcgtccgt gccgacccgc atatcggcct tctgcaccga ggcacagaga aactggctga
    10741 aaccaaaacc tacctgcaag ccctgcccta tatggaccgt ttggactacg tttccatgat
    10801 ggtcaacgag caggcgtatt gcttggcggt agaaaaactt gccggtatcg atgtgcctat
    10861 ccgcgcccaa tacatccgcg tgatgtttgc cgaagtaacg cgcatcctca atcacttgat
    10921 gggtatcggt tcgcacgcct tcgacatcgg cgcgatgacc gccatccttt acgccttccg
    10981 cgaccgcgaa gagctgatgg atttgtacga agccgtgtcc ggcgcgcgta tgcacgccgc
    11041 ctacttccgt cctggcggcg tttaccgcga cctgcccgac ttcatgccca aatatgagag
    11101 cagcaaattc cgcaacgcca aagtattgaa gcagctcaac gaatcccgcg aaggcaccat
    11161 gctcgacttt atcgacgcct tctgcgaacg tttcccgaaa aacatcgata cactcgaaac
    11221 cctcctgact gacaaccgta tttggaaaca gcgtaccgtc ggcatcggcg ttgtcactcc
    11281 tgagcgcgcc atgcaaaaag gctttaccgg cgttatgttg cgcggttcag gcgtggaatg
    11341 ggacgtgcgt aagacacagc cttatgaagt gtacgacaaa atggatttcg acatccccgt
    11401 cggcgtcaac ggcgactgct acgaccgtta cctctgccgt atggaagaaa tgcgccaatc
    11461 cgtacgcatc atcaaacaat gttccgagtg gttgcgtgtc aatccgggtc cggtcattac
    11521 cacaaaccac aaattcgctc cgcccaaacg taccgaaatg aaaacaggta tggaagacct
    11581 gattcaccat ttcaaactct ttaccgaggg tatgcacgtt cccgagggcg agacctacac
    11641 cgctgtcgaa catccgaaag gcgagttcgg cgtttacatc atttcagacg gcgcaaacaa
    11701 accctaccgc ctgaaaatcc gcgcacccgg cttcgcccat ctgcaaggca tggacgaaat
    11761 ggcaaaaggc cacatgctcg ccgacgtcgt tgccatcatc ggtacgcagg acatcgtatt
    11821 cggggaggtt gaccgataat gttatccgca gaatccttaa aacaaatcga catcgagttg
    11881 gcgaaatatc ccgccgacca acgccgctct gcgattatgg gcgcattgcg tattgcccag
    11941 accgaaaaag gctggctcgc ccccgaaacc atcgctttta tcgccgacta catcggcatc
    12001 tcgcctgcac aagcctacga agtcgctacc ttctacaata tgtacgacct tgagcctgtc
    12061 ggcaaataca aactgaccgt ttgtaccaac ctgccctgcg ccctacgcgg cggtatggcg
    12121 accggcgaat acctcaaaca aagactcggt atcggctacg gcgaaaccac gcccgacggc
    12181 aaatttaccc ttgtcgaagg cgaatgcatg ggcgcgtgcg gcgacgctcc ggttatgctg
    12241 gtcaacaacc acagcatgtg cagctttatg accgaagagg cgattgagaa gaaactggcg
    12301 gagttggagt aggtcgtctg aaacgacgat ttaaacgtag gtcggatact tgtatccgac
    12361 agagtgggta aaaaggcaaa atgtcggatt taagaatccg ccctactgaa ataccgaaat
    12421 gccgtcattc ccgcgcaggc gggaatccac cggtaagatt cggtttctga atttaataag
    12481 acattgctta ccattgagga tggattcccg cctgcgcggg aatgacgaca gacaagcaag
    12541 tggtcgagat ccaacaaaaa cgattaaagg tcgtctgaaa atatcgattt gataaactag
    12601 attttatttc agacgacgtt acaagccggt acacaaagac atcttaaggt cgtctgaaac
    12661 agcggccgca accgatacga aaacaaacag gcacaccaaa aatggctatt taccaatcag
    12721 gcgtgatttt tgaccaagtg gataccgcca atcccgactg ctggacattg gacgaatacg
    12781 tcaaacgcgg cggctatacc gccctgcgta aaattctgtc cgaaaacatc tcgcaaaccg
    12841 atgtgattga cgaagtcaaa acctccggct tgcgcgggcg cggcggtgcg ggcttcccga
    12901 ctggtttgaa atggagcttt atgccccgtt ccttcccggg cgaaaaatat gtggtttgta
    12961 acaccgacga aggcgaaccg ggtacattta aagaccgtga catcatcatg ttcaatcctc
    13021 atgccctgat tgaaggcatg attatcgccg gttacgcgat gggtgcgaaa gccggctaca
    13081 actatatcca cggcgaaatc ttcgaaggct accaacgctt tgaagccgct ttggagcagg
    13141 cgcgtgccgc aggctttttg ggtaaaaata ttttgggttc ggattttgaa tttgaactct
    13201 tcgcccacca cggctacggc gcatatattt gcggcgaaga aaccgcattg ctcgaatcgt
    13261 tggaaggcaa aaaaggccag ccgcgcttca aaccgccatt ccctgcttcg ttcggcctgt
    13321 acggcaaacc gaccaccatt aataatactg aaaccttttc ctccgtccca ttcattatcc
    13381 gtgacggcgg acaggcattt gccgataaag gtattccgaa tgcaggcggt accaaattat
    13441 tctgtatttc cggccatgtc gagcgtccgg gcaactatga agtgccattg ggtacgccgt
    13501 ttgccgaagt cttgaaaatg gcgggcggta tgcgcggcgg taaaaaactc aaagccgtca
    13561 ttcccggcgg ttcgtccgcg cccgtattgc ctgccgacat catgatgcag accaatatgg
    13621 actacgactc gatctccaaa gcaggctcca tgctcggttc cggcgcgatt atcgtcatgg
    13681 acgaagacgt gtgcatggtc aaagcccttg agcgtttgag ctacttctac tacgacgagt
    13741 cttgcggcca atgtaccccc tgccgagaag gtacgggctg gctttaccgc atcgtccacc
    13801 gcatcgtaga aggcaaaggt aaaatggaag atttggattt gctggattcc gtcggcaacc
    13861 aaatggcagg ccgcaccatc tgcgccctcg ccgatgctgc cgtcttcccc gtccgcagct
    13921 ttaccaagca tttccgtgat gagtttgtgc attacatcga acacggcggg ccgatgaaag
    13981 agcataagtg gggagggtgg taatggtgga agctaaaatt tttattctat acggtgcagc
    14041 caacaaaggt aagagtacga cactcaatac gctttttaat cagatttgtc ggaaattttc
    14101 taaatttcta gtcttttttg aaagacatgg aaacggctta gattttgttg cagtatttga
    14161 tcatgaaggt cagagaattg gtttttattc atctggtgat aatgaatacg aggttagggg
    14221 aaatttatac aaactttatt cgcataattg tgattttatt tttggcacgt caaggacacg
    14281 gggtggtagt tgcgatgcag taggatgtta tgcagagtta ttgcatggcg atgtaaatat
    14341 aattaattgg tgtgaaaagt ttgagcctac agatgaagac aatgagcgtg ctgttaaaga
    14401 gttatttaag tcatttaaaa atataataaa tgagttatag ttttagttgg ttttatattg
    14461 gttaaaagca aaatgctaaa aatttaactt tgccgtcatt cccgcgtagg cgggaatcca
    14521 tagtggaatt tacagaaccc gatatttgaa aagcagttgc cgaaattcaa aaaatggatt
    14581 cccgcctacg cgggaatgac ggcaaggtgg cagtaaacgt tttaaacagt attgattgtc
    14641 aatgaaactc aaaaggccgt ctgaaaccca tttttcagac gacctccata aaagattatt
    14701 tatcaaatac ccgtaactag gaacgaacca tgttacaaat cgaaatcgac ggcaaacaag
    14761 tatctgtgga gcagggcgcg acggtgattg aagccgcgca caagctcggt acttatattc
    14821 cgcatttctg ttaccacaaa aaactttcca tcgccgccaa ctgccgtatg tgtctggtga
    14881 acgtagaaaa agccccaaaa cccctgcctg cctgtgccac gccggttaca gacggcatga
    14941 ttgtgcgtac gcattcggca aaagcccgag aggcgcagga aggcgtgatg gagttcctgc
    15001 tcatcaacca tccgcttgat tgtccgacct gcgaccaagg cggggaatgc cagttgcagg
    15061 atttggcggt gggctacgga aaaaccacca gccgctacac cgaagaaaaa cgttccgtcg
    15121 tcggcaaaga tatggggtcc ttggtttccg ccgaggaaat gagccgctgt atccactgca
    15181 cccgctgcgt tcgtttcact gaagaaatcg ccggtttgca ggaaattgcg atggtgaatc
    15241 gcggcgaaca ctccgaaatc atgcccttta tcggcaaaac ggtggaaacc gaattgtcgg
    15301 gcaacgtcat tgatttgtgt cccgtcggcg cgctgaccag caaaccgttc cgcttcaatg
    15361 cgcgtacttg ggaattgaac cgccgcaaat ccgtttccgc ccacgacgct ttgggcagca
    15421 acctgattgt acaaaccaaa gaccataccg tccgccgcgt gttgccgctg gaaaatgaag
    15481 cgattaacga atgttggctg tctgaccgcg accgtttttc ctacgaaggc ctgtatcacg
    15541 aaagccgtct gaaaaaaccg aaaatcaaac agggcggcga gtggatggac gtggattgga
    15601 aaaccgcgtt ggaatatgtc cgcagcgcga ttgaatgtat cgccaaagac ggcaaccaaa
    15661 accaagtcgg catttgggcg aacccgatga atacggttga agagctgtat ctggccaaaa
    15721 aacttgccga cggcttgggt gttaaaaact ttgccacccg tttgcgccaa caagacaaac
    15781 gtctttcaga cggccttaaa ggtgcgcaat ggttgggaca aagcattgaa tcgctggcgg
    15841 acaacgatgc cgtattggta gtcggtgcga acctgcgcaa agaacagccg ctcctgaccg
    15901 cccgcctgcg ccgcgccgcc aaagaccgta tggcgttgag tgtattggcc ggcagtaaag
    15961 aagagttgtt gatgccgctt ctctctcaag aagccgcaca tcccgacgag tgggcaggcc
    16021 gtctgaaaaa cctgtctgcc aatgcggaac acgccgttac cgccagcctg aaaaacgctg
    16081 aaaaagcagc ggtgattttg ggcgcggaag tgcaaaacca tcctgattac gccgccattt
    16141 atgccgccgc gcaagagctg gcggatgcaa ccggcgcggt actgggtatt ttgccgcaag
    16201 ccgccaacag cgtcggtgcg gacgtattgg gcgtgaactc aggtgagagc gttaccgaaa
    16261 tggcaaacgc gccgaaacag gcagtcttgc tgctcaacgt cgagcctgaa attgatacgg
    16321 ttgacggtgc aaaagccgta gccgcgttga aacaggcaaa aagcgtgatg gcgtttacac
    16381 cgtttgtcag cgaaacgctg ctggacgtgt gcgacgtgtt gttgccgatt gcgccgttta
    16441 ccgaaacctc aggcagcttc atcaatatgg aaggccgtct gcaatctttc cacggcgtgg
    16501 tacaaggctt cggcgattcg cgtccgatgt ggaaagtgtt gcgcgtattg ggcaatctgt
    16561 ttgacctgaa aggttttgaa taccacgata ccgctgcgat tttgaaagac gcgctggatg
    16621 cagaaagcct gtcgtccaaa ctggacaacc gcagtacatg gacaggggag ggcgttcaga
    16681 cggcatcaga ccgcctcgtc cgtgtcggcg gcgtcggtat ttatcacacc gatgccatcg
    16741 tacgccgttc cgcaccgttg caagaaacca gccatgccgc cgtgcctgcc gcgcgtgtaa
    16801 atccgaacac attggcacgc ttgggcctgc aagacggaca aaccgctgtc gccaaacaaa
    16861 acggcgcaag cgtatcggtt gctgtcaaag ccgatgccgg tttgcctgaa aacgtggtgc
    16921 atctgccgct gcataccgaa aatgccgcgc tgggtgcgtt gatggacact attgaactgg
    16981 cgggagcttg atcatgcaag aatggttcca aaacctcttt gccgcaacgc tcggtctggg
    17041 cgatttgggc atcaccgtag gcttggtggt atccgtcatc gtcaaaatcg tgattatcct
    17101 gattccgctg attctgaccg ttgcctacct gacgtatttc gaacgtaaag tcatcggctt
    17161 catgcagctt cgcgtcggtc cgaacgtaac cggcccgtgg ggtctgattc agccgtttgc
    17221 cgacgtgttc aaactcttgt ttaaagaggt aacccgtccg aagctgtcaa acaaagccct
    17281 gttctacatc ggcccgatta tgtcgcttgc tccgtctttc gcagcatggg cagtgattcc
    17341 gttcaacgaa gaatgggtgc tgaccaacat caatatcggc cttttgtaca tcctgatgat
    17401 tacctcgctg tcggtttacg gcgtgatcat cgcgggctgg gcttccaact ccaaatattc
    17461 gttcttgggt gcaatgcgtg cttccgcaca aagcatttcc tacgagattg ccatgagtgc
    17521 cgcgctggtg tgcgttgtga tggtatcggg cagcatgaac ttctccgaca tcgttgccgc
    17581 acaagccaaa ggcatcgcgg gcggttccgt gttctcgtgg aactggctgc cgctcttccc
    17641 catcttcatc gtttatctga tttccgccgt tgccgaaacc aaccgtgcgc cgtttgacgt
    17701 ggcagagggt gagtctgaaa tcgttgccgg tcaccacgtc gaatattccg gcttcgcgtt
    17761 cgcgctgttc ttccttgccg aatacatttt catgattctg attgccgcgc tgacatcgct
    17821 gatgttcctc ggcggctggt tgtccccgtt cccgcaaagt tggggcttta tcggcacacc
    17881 ttccgcattc tggatgttcg tgaaaatggc ggcggtgttg tactggtacc tgtggatccg
    17941 tgcaaccttc ccgcgctacc gttacgacca aatcatgcgt ttgggctgga aagtgctgat
    18001 tccgatcggc ttcgcctaca tcgtggtttt gggcgtgtgg atgatttcac cgctgaattt
    18061 gtggaaataa gtttcagacg gcatcttgag gccgtctgaa caaagcgatt ttgaatacct
    18121 aacgaaatcc ctgttttgag ggaacataat atggctaact tagtaaaaac ctttctgctt
    18181 ggcgaattgg taaaaggtat gggcgtaacg ctcaaaaact ttttcgcccg caaagacacg
    18241 atttattttc ccgaagagaa aacgccgcaa tccgtgcgtt tccgcggcct gcacgcgcag
    18301 cggcggtatc cgaacggtga ggaacgctgc attgcgtgta agttgtgcga ggcggtgtgt
    18361 ccggcaatgg cgatcaatat cgaatcggaa gaacgcgaag acggcacccg ccgcaccaag
    18421 cgttacgaca tcgacctgac caagtgcatc ttctgcggct tctgcgaaga ggcctgccca
    18481 accgatgcga ttgtggaaac gcatattttt gaataccacg gcgagaaaaa aggcgacttg
    18541 cacatgacca agccgattct tttggccatt ggcgataaat acgaagctga aatagccaaa
    18601 cgcaaagccg ctgacgcgcc gtatcgttaa tgctttgggg cttcttggaa ggttttaaat
    18661 atggaaggac tgattaatgc attgaaatat ttagccgaac atgagccaat agataatttt
    18721 gaagaaatta gaactagaaa tagtccgatt gagttgccaa gtggattaag taattttgaa
    18781 caaaatattt ttttaaaaga aaatttatcc ccaaaattac aaaatgatga tagcttgaag
    18841 acgcattatt ggattatccg tgaatggggt gggattaaaa gttttaaaca atctgctgaa
    18901 aatagccagc ttattcgtca atttttatcg gaacttaatt cgggaaaatt gagtagtggt
    18961 ttgttgaaaa tttcatcatt atctaaattg gcttctttta tagattgtga gcgattcgcc
    19021 atttatgatt cacgcgctat tttttcgttg aattggttgt tgtttaaatt tacaaatgca
    19081 gatttgtttt ttcagccaca aggtagaaat agggaactag aaatccgaaa tatgaacgta
    19141 ttgtttcatt tttctgatat caaaccgaat tatcggaaac cagacgtttc gtttcatcaa
    19201 tattgtggat tgttacaaga tttggcgaaa caagtttatg gtaaacaagc aaaaccgtat
    19261 cacatagaaa tgttgttatt caaaattgcg acaacgtgga tttgtgcgga tatggatcaa
    19321 ctgattaagt ttgattgttt gcgtaaccag gattttcaga ctgcttgaaa ccatattttt
    19381 gattaataaa gaaagcatag actatgactt tccaactgat tttattttat atttttgcag
    19441 tgataattct ttatggcgcg ctcaaaaccg ttaccgccaa aaaccccgtg catgccgctt
    19501 tgcatctggt gctgaccttc tgcgtgagcg cgatgatttg gatgctgatg caggcggagt
    19561 ttttgggcgt tacgctggtg gtcgtgtatg tcggcgcagt gatggtattg ttcctgtttg
    19621 tcgtgatgat gctgaatatc gacatcgagg aaatgcgcgc cggtttttgg cggcacgcgc
    19681 cggttgccgg cgtggtcggt acattgttgg cggttgccct gattctgatt ttggtcaacc
    19741 cgaaaaccga ccttgccgca tttggtctga tgaaagacat tcccaccgat tacaacaata
    19801 tccgcgattt gggcagccgt atttataccg aatacctgtt gccgtttgag ctggcggcgg
    19861 tattgctgct gttgggtatg gtggcggcga ttgcgctggt tcaccgtaaa accaccaatc
    19921 cgaaacgcat ggatcctgcc gaccaagtta aagtacgtgc cgaccaaggc cgtatgcgtc
    19981 tggtgaaaat ggaagcggtc aaaccgcaag ttgaatctgc cgaagagagc gaagtttcag
    20041 acggcttcaa gccggaaggg gagggtaaag catgattacc ttgacgcatt atctggtatt
    20101 gggtgcgctc ctgttcggta tcagcgcaat gggtatcttt atgaaccgca aaaacgtgct
    20161 ggtattgctg atgtccatcg agctgatgct tttggcggtg aacttcaact ttatcgcctt
    20221 ctcgcaacat ttgggcgata ctgccggaca aattttcgta ttcttcgtat tgaccgttgc
    20281 cgctgccgaa tccgccatcg gtttggcgat tatggtattg gtgtaccgca accgacaaac
    20341 gattaatgtt gccgatttgg atgggttgaa agggtaaagg taggttgggt cgagacctga
    20401 caagacaccg atgccgtctg aaaacccgat aggaaaaacg atgaaatcca tagacgaaca
    20461 aagcctgcat aatgcccgcc gcctgtttga aagcggcgac atcgaccgta tcgaagtcgg
    20521 taccaccgcg ggcctgcaac agattcaccg ttacctgttc ggcggcttat atgattttgc
    20581 gggtcaaatc agggaagaca acatttccaa aggcggtttt cgttttgcca acgccatgta
    20641 tttaaaagag gctttggtta aaatcgagca gatgcccgag cggacttttg aagaaatcat
    20701 cgccaaatat gttgaaatga acattgccca tccgtttttg gagggtaatg gcagaagtac
    20761 ccgcatctgg ctggatttgg tgctgaaaaa aaacctgaaa aaagtcgtga actggcaaaa
    20821 tgtaagtaaa accctgtatt tgcaggcgat ggaacgcagc cccgtcaacg atttagaact
    20881 gcgctttctg ttaaaggaca acctgactga cgatgtggac aaccgtgaaa tcatctttaa
    20941 aggtatcgag cagtcgtatt attacgaagg gtatgaaaaa ggctgagggt cgtctgaaaa
    21001 gcgatttcag actgtttcag acgacctgat tcggtaggtg atcagacggg agcggatgag
    21061 aaaagaaatt ctgggtaaga ataatccggt ctgaaatatt ggaagaagaa tgatggataa
    21121 aaatcagtta gaacaagaat ttcataaagc catgttaaat atttatcagg aggctttgaa
    21181 tttgccgcaa ccttacaagg cgacacgatt tttacaaatt gtaaatgaat ttggtggtaa
    21241 agaggcggcg gataaattat tgagtacggg ggaaaagaag actcagaccg gttttacaga
    21301 gctgattctg agtggtggcg gagtccacgc cttgaaatac agtatggaat atctggtgtt
    21361 acaaaagccg tggtgtgatt tatttactga agagcaatta gctgtggcac gcaaacgatt
    21421 ggagcgtgtt ggatttgttt ttccgaagta attttgtacg aaacaaacat agatttttaa
    21481 atcaatcgga ttcaatcaaa tgaacgacat gactttatat ctcatcattg cccttgttcc
    21541 gttggcaggc tcgttgattg cgggtttgtt cggcaacaaa atcggtcgtg ccggtgcgca
    21601 tactgttacg atactcggtg tggcggtgtc tgccgtgctg tcggcttatg tgctgtgggg
    21661 attcctcaat ggcagccgtg ccaagtttga cgaaaacgtc tatacctggc tgacaatggg
    21721 cggcttggat ttttccgtcg gcttcttggt tgatacgatg acggcgatga tgatggtcgt
    21781 ggtaacgggc gtgtcgttga tggtgcatat ctacaccatc ggctatatgc acgacgaaaa
    21841 agtcggctac caacgcttct tcagctatat ttctttgttt actttcagca tgttgatgct
    21901 gattgtgagc aacaacttca tccagctctt cttcggctgg gaggctgtgg gcttggtgtc
    21961 gtatctcttg atcggtttct atttcaaacg tccaagcgca acatttgcca acctgaaagc
    22021 ctttttgatc aaccgtgtcg gcgacttcgg ctttttgctc ggtatcggct tggtgcttgc
    22081 ctatttcggc ggcagcctgc gctatcaaga tgtattcgct tatctgccca acgtgcaaaa
    22141 tgccaccatc cagcttttcc ccggtgtgga atggtcgttg attactgtca cctgtttgct
    22201 cctgtttgtc ggtgcgatgg gtaaatcggc acaattccca ctgcacgttt ggctgcctga
    22261 ttcgatggaa ggcccgaccc cgatttctgc attgattcac gccgcaacca tggttaccgc
    22321 cggtttgttt atggtgtcgc gtatgtcgcc gatttatgag atgagcagca ccgcgctgtc
    22381 tgtcatcatg gtaatcggtg cgattaccgc cctgtttatg ggtttcttgg gcgtgattca
    22441 aaacgacatc aaacgtgtgg ttgcgtattc caccctgtcg caactgggct atatgaccgt
    22501 ggctctgggc gcgtctgcct attccgtggc gatgttccat gtgatgaccc acgccttctt
    22561 taaagccttg ttgttcttgg cggcgggcag cgcgattatc ggtatgcacc atgaccaaga
    22621 tatgcgccac atgggcaatc tgaaaaaata catgccgatt acttggctga ccatgctgat
    22681 cggtaacttg tcgctgatcg gtacgccgtt cttctccggc ttctattcca aagattcgat
    22741 tatcgaagcg gcgaaataca gcaccctgcc gggcagcggc tttgcctatt ttgccgtcct
    22801 cgccagcgtt tttgttactg cgttctacgc attccgccaa tactttatgg tgttccacgg
    22861 cgaagagaaa tggcgcagct tgcctgaaca tcatgacgat catcatggcg aagaacatca
    22921 cagcttgggc aaaaacgaca atccgcacga aagcccgttg gttgttaccc tgcctttgat
    22981 tttgcttgcc attccgtccg tcatcatcgg ctaccttgcc atcgagccta tgctttacgg
    23041 cgatttcttc aaagatgtga ttttcgtcaa cgccgacgcg catccgacca tgcacatcat
    23101 gaaggaagag ttccacggcg cattggcaat ggtgtcgcac agcctgcatt cgcccgtact
    23161 ctaccttgct atcgcaggcg tgttgagcgc atggcttttg tacgtcaaac tgccgcacct
    23221 gccagcgaaa attgcacaga cgttccgtcc ggtttacgtt ttgtttgaaa acaaatacta
    23281 cctcgacgtc ctgtatttca acgtctttgc caaaggcacg cgcgcattgg gcactttctt
    23341 ctggaaagtc ggcgataccg ccattatcga caacggcatc gtcaacggct ctgccaaact
    23401 ggtcggcgcg attgccgcgc aagtgcgtaa agctcaaacc ggctttatct atacctacgc
    23461 cgccgctatg gtattcggcg tattggtact gctcggcatg accttctggg gattgttccg
    23521 ataagaataa ggtttcagac ggccttaaac cttcaggccg tctgaaacga agaaatatcc
    23581 acataaacac atttttattt taaccacagg ttaaccacta tgttttccaa ctacctactc
    23641 agcttggcaa tatggatacc catcgccgca ggcgtgctgg ttttggcaac ggggtcggac
    23701 agccgtgcgc cgtttgcccg cgtgctcgcc ttcatgggtg cgcttgccgg tttcttggta
    23761 acactgcccc tgtttaccgg tttcgaccgt ttgagcggcg gctatcaatt taccgagttc
    23821 cacgagtgga ttccgcttct gaaaatcaac tacgcattgg gcgtggacgg tatttcagtg
    23881 ctctttatca tcttgaatgc gtttattacg ctgttggtgg tattggcagg ttgggaagtc
    23941 attcagaaac gtccggcgca gtatatggcg gcattcctga tcatgtcggg tttgattaac
    24001 ggcgcgtttg ccgcgcagga tgcgattctg ttttatgtgt tcttcgaggg tatgctgatt
    24061 ccgctgtacc tgattatcgg tgtatggggc ggtccgcgcc gcgtctatgc gtcggtcaag
    24121 ctcttcctct acacgctgat gggttcgctc ctgatgctgg ttgcgatggt ttacctttat
    24181 tatcaaacag gcagcttctc tattgtcgat ttccaaaaca tcgaacagat tccgttgggc
    24241 gtacaacagc ttttgtttgt ggcgttcttc ctgtcatttg ccgtaaaagt gccgatgttc
    24301 cctgtgcaca cttggttgcc ggatgcccac gttgaagcgc cgaccggcgg ttcgatggtg
    24361 ttggcggcca ttacgctgaa actgggtgcg tatggtttct tgcgctttat cctgccgatt
    24421 atgccggatg cggcacgcta ttttgccccc gtgatcatcg tattaagtct gattgccgtg
    24481 atttatatcg gtatggtggc tttggtgcaa accgatatga aaaaactggt ggcgtattcg
    24541 tccatcagcc atatgggttt tgtaacgctt gggatgtttt tgtttgttga cgggcagttg
    24601 gacgactggg cattgaaagg tgcaatcatt caaatgattt cgcacggttt cgtgtctgcc
    24661 gcgatgttta tgtgtatcgg cgtgatgtac gaccgcctgc acacgcgcaa tattgctgat
    24721 tatggcggcg tggtcaatgt gatgcccaag tttgcggcgt ttatgatgct gttcggtatg
    24781 gcgaacgcgg gtttgcctgc gacttccggc ttcgtgggcg agtttatggt gattatgggc
    24841 gcggtcaaag tgaatttctg ggtcggcgcg ttggccgcca tgaccctgat ttacggtgca
    24901 tcttataccc tgtggatgta caaacgcgtt atttttggtg cgatccacaa tccgcacgtt
    24961 gccgaaatgc aagacatcaa ttgccgcgaa tttgcgattt tggcaatttt ggcggtggct
    25021 gttttgggta tgggcctgta tccgaacgca tttatcgaag tggtgcatca ggcggcaaac
    25081 gatttgattg cccatgtggc acaaagcaag atttgaggtg tgtaaatgaa ctggtctgat
    25141 ttgaatttaa tgcccgccat gcccgaaatc gtgctgctgt cgctgctggt gttattgttg
    25201 ctggcggact tgtgggtcag tgatgacaaa cgccggtgga cgcattacgg cgcgttggca
    25261 acggtggcgg ttacggctgt ggtgcagttg gcggtgtggg aacagggcag cacgtcttcg
    25321 ttcaacggga tgtatattgc agacggtatg tcgcgtttgg caaaaatggt tttatatgcc
    25381 ttgacctttg ccctgtttgt ctatgccaag ccctacaacc aagtgcgcgg tatttttaaa
    25441 ggcgagtttt acaccctgtc attgtttgcc ctgttgggta tgagtgtgat ggtgagcgcg
    25501 gggcattttt taactgccta tatcggtttg gaactcttgt cgcttgccct ttacgccctg
    25561 attgccctgc gccgcgattc cggctttgcc gccgaagccg ccttgaaata ttttgttttg
    25621 ggcgcgctgg catccggcct gctgctctac ggtatttcta tggtttacgg cgcaaccggt
    25681 tcgctggaat ttgccggcgt gctcgcctct tccttcaatg aagaagccaa cgaatggctg
    25741 ttgaaactgg gtttggtgtt tatcgtcgtc gccgtcgcgt tcaaactcgg tgcggtgccg
    25801 ttccatatgt gggtgcccga cgtgtatcac ggcgcgccca cttctgttac cgccttggtc
    25861 ggcactgccc cgaaaatcgc cgccgtcgtt ttcactttcc gcatcctcgt taccgggctg
    25921 ggaaccgtgc atcatgactg gtctctgatg tttgccctgc ttgccgccgc ctcgctgctg
    25981 gtcggcaacc ttgccgccat catgcagacc aatatcaaac gtatgttcgc ctattccacc
    26041 gtatcgcata tgggtttcat cctgttggcg tttatggcgg gcgcggtcgg ctttgcggcg
    26101 ggcctctatt acgccattac ctacgcgctg atggcggcgg cagggttcgg agtgttgatg
    26161 gtgttgtcgg acggggacaa cgagtgcgaa aacatcagcg atttggcagg gttgaaccaa
    26221 caccgcgtat ggcttgcctt tttgatgctg ctggttatgt tctctatggc gggcattccg
    26281 ccgctgatgg gtttttacgc caaattcggc gtgattatgg cactcttgaa acaaggccat
    26341 gtttggttgt ctgtatttgc cgtcatcatg tcgctgattg gtgcgttcta ctacctgcgc
    26401 gtggtcaaag tcatctactt cgatgtgcct gatcatgacc agccggtcgg cagcaactat
    26461 gccgccaaat ttgttctgac ggtcaatgcc ttcttgctgc tcctgtgggg catcatgccg
    26521 caaaccgtta tcgactggtg cgccaaggcg ttggagaaca cgctgtaagc cgccgcaacg
    26581 gcagccgtgt cagaggctgc cgtttttgtt aagatatgcc gttccgcaac gcggttcaga
    26641 cggcatcgcc gccgacaacg cctaaacaga aagcccacca tgaccgcatc catgtacatc
    26701 cttttggtct tggcactcat ctttgccaac gcccccttcc tcacgaccag actgttcggc
    26761 gtggccgcac tcaagcgcaa acatttcgga caccacatga tcgagctggc ggcaggtttc
    26821 gcgctgaccg ccgttcttgc ctacatcctc gaatcccgtg caggatcggt acacgatcag
    26881 ggttgggagt tttatgccac agtcgtctgc ctgtacctga tttttgcgtt tccatgtttt
    26941 gtgtggcggt atttttggca cacgcgcaac agggaataga caagcatagg aatgccgtct
    27001 gaaacccttt cagacggcat ttgtttcatt caagtgcagg ccggcatcgc tgtgccggca
    27061 cgtttcagcc ggcgatatac gccggtttta atatttgcgg gcgactgcaa attctgccaa
    27121 ctgccgcagg cgcagggctt tgtcgccgaa gggttcgagc agcgcgaccg cttcggcaac
    27181 cagtttgtgt gcgtatgagc gcgccgcttc caagcccatc agtttcacat aagtcggctt
    27241 gtcgttgtct gcgtctttgc ccgccgtttt gcccaaagtc gccgtgtccg cttcacaatc
    27301 caacacatcg tcaatgactt ggaacgccag ccccagtttt gccgcgtaag cgtccaatac
    27361 ggaaagttcc gcatctgaca gatcaggaca cgccgtcgcc cccaataaaa ccgccgcacg
    27421 gattagcgca cccgttttca ggctgtgcat ctgttccaaa tcggcttgaa ccatttgttt
    27481 gccgacattc gccaaatcga ttgcctgacc gcccgccata cccctgctgc cgcccgcttt
    27541 cgccaacacc gacaacattg ccaactggcg tgcggcgggc agttctgtcg gacggctcaa
    27601 cacgtcaaat gcctgtgtct gcaaagcgtc gccggtcaga agggcggtcg cttcgccata
    27661 tttgatgtgg caagtcggtt tgccgcgccg caggctgtcg ttgtccatcg ccggcatatc
    27721 gtcgtgaacc aaagaataga cgtggatcat ttcgattgcc gccattgcct gttctactgc
    27781 ttcatgcacg gcttcgccta attccgaagc tgccagaacc agcatcggcc gcagacgctt
    27841 accgccgtcc aaagccgcat aacgcatcgc ttcgtgcagt gtgtgcggta tttccccctc
    27901 agacggtaaa aaccgttcaa gcagcagctc tgtttgcgcc tgcgccctct gttgccacgt
    27961 tttcaaatca ttcgtcggat tcaaggttta actccttcag cccgtctgtg tctaaaacct
    28021 gtagcttttg ttcgacttgt gccagtttgg tttggcagta cctgaccagt tcgttgcctt
    28081 cctgataggc ggcaagcgcg tcttccaagg gcatttcgcc ctgcatagac tgcgtcagcg
    28141 attcgaggcg cgacaaggct tcttcaaacg atttcggggc gtttttcttc atcgtatttc
    28201 cttttcggtt gaaaccccgc cctttagggc ggcaggatca gactttattt gggaggggtg
    28261 taaccctttc caaatcaggg caatacatag ggcggtgctt tatgtgccgt cctgtgtgtt
    28321 ggaacatagt ttcggatgtt ccggtaaaaa gcggattgta gcatttttga aaaacggatg
    28381 ccgtctgaaa cccgaatccg gcttcagacg gcattttttc cgcccaggcg gcaaggcgtt
    28441 acccgggcag ttcgtcggtg atgccctgca aaaaggcgag gcgttcgggg cttgccgccc
    28501 cggtttgcgc ggcggctttg aaggcgcagc cgggttcggc gcggtgggtg cagttgtgga
    28561 agcggcattg cccgacaagg tggcggaaat cggggaaata gcgcggcaaa tcggcggctt
    28621 ggaggtggtg taaaccaaat tcttgcaaac ccggggagtc gatgagttgg gtttcgccgt
    28681 tcaaatcata aagccgggcg tgggtggtgg tgtgttttcc cgagtcgagt gcggcggaaa
    28741 tgtcgccggt gcgggcggtt tggctgccca aaagggcgtt ggtcagggtg gatttgccca
    28801 taccgctctg cccgagcagg atgttgctgt gcccttgcag ggcggggcgc aggctgccgg
    28861 cgttttccag tgcgcgggtt tcgatgacgg gataacccag cgtttcgtag aatttgagtt
    28921 tttcgcgcca aagggcggtt tcgggcaggt cggctttgtt caggacgatg acggcttcaa
    28981 taccggcggc ttcggcggca agcagggcgc gttgcagcag ccgcacgctc ggactcggga
    29041 cggcggcggt tacgatgagg agttgggtaa cgttggcggc gatgagtttg gttttccacg
    29101 cgtcttggcg gtagagcagg ctttggcgcg gtaaaaaatc ttcaatcaca acttgttcgg
    29161 cgttgacggg gctgatgcgg acgcggtcgc cgcaggcgaa atcgacgcgt tttttgcggg
    29221 tgctggcttc gtaggttgtg ccgtcgggcg tgcggacaat gtagcggcgg ccgtagctgg
    29281 cggtaatttg ggcggtgtcg ttcatggttt ctttggggtt gggtgtggga atgccgtctg
    29341 aaaacgggtg ttcggacggc atcggttcag tcgtgctgcc actcgacgtg ttcgttgagg
    29401 aagccgccgc tctggtgcgc ccagagtttg gcgtaaagcc cgcgtttttc gaggagttcg
    29461 gcgtgtgtgc cttcttcgat gatgcggcct ttgtcgagga cgacgagcct gtccattgcg
    29521 gcgatggtgg agaggcggtg ggcgatggcg atgacggttt tgccgtccat cattttgtcg
    29581 aggctttctt ggatggcggc ttcgacttcg gaatcgagcg cgctggtggc ttcgtccaaa
    29641 agaagaatcg gtgcgtcttt gagcatcacg cgggcgatgg cgatgcgctg gcgttgcccg
    29701 ccggagagtt tcacgccgcg ttcgccgacg tgtgcgtcgt agccgcgccg ccctttggca
    29761 tcggaaaggt cggggatgaa gccggcggct tcggcgcgtt cggcggcaga aaccatttcg
    29821 gcatcggtcg cgtcggggcg gccgtaaata atgttgtcgc gcacggaacg gtgcagcagc
    29881 gaggtatctt gcgtgaccaa accgatttgg gcgcgtaaag attcttgggt aacgccgctt
    29941 atgtcctgcc cgtcgatcga aaccgtgccg ctttgcggtt cgtagaagcg caaaagcagg
    30001 ttgacgatgg tggatttgcc cgcgccgctg cgtccgatca agccgacttt ttcgcccggg
    30061 cggatggtga ggttgaagcc gttgagcagc ggtttgcccg cttcgtagga gaaatcgacg
    30121 tgttcgaact tgatttcgcc ttgcgacact ttcagcggca gggcgttcgg cttgtcgagg
    30181 atggtgtgcg gtttggacag ggttgccatg ccgtcgccga cggtgccgat gttttcaaac
    30241 agccgcgcgg attcccacat aatgtattgc gacaaaccgt tgacgcgcaa cgccatcgcg
    30301 gtggcggtgg cgaccgcgcc cacgccgacc tgtccgttgt gccaaagcca gatgcccagt
    30361 gcggcggtgg agagggtcag ggaggtgttg acgatgaagc tgcacgaatg cagcagcgtc
    30421 gccagccgca tttgggcgcg caccgtaacc ataaattctt ccatcgactg cttggcatag
    30481 gcggcttcac gcgcgccgtg ggagaagagt ttgacggtgg cgatattgga ataggcatcg
    30541 gtaatgcggc cggtcatcag cgagcgggca tccgcctgcc atgcggcggt ttgccccaat
    30601 ttgggaatca gcaggcgcat caccgaagcg aaaccgacaa tccagccgat aaagggcagc
    30661 agcagccatg agtcgagcga ggcgagaatc acgccggagg taatgaaata caccgacaca
    30721 taaacgacca tatcggcaac cgtcatcacc gcgtcgcgca acgccagcgc ggtctgcatg
    30781 actttggcgg acacgcgtcc ggcaaattcg tcctgataaa aaccgaggct ttggttcagc
    30841 atcaggcggt ggaagttcca gcgcaggcgc atggggaaca cgccctgaag ggtttgcagg
    30901 cgcacgttgg acgcggcaaa cgcccacacg accgaaaata ccatcatcgc cgccattgcc
    30961 gccagttccc aacctttttc ggcaaacagt tcggcgggcg cgtatttgcc gagccactcc
    31021 acgattttgc ccataaattg aaaaatcagg gcttccataa tgccgatgcc ggcggtcagc
    31081 gcagccaggg cggctatcca tttccgcacg ccggccatgc tgctccagac aaaccgccac
    31141 aagccttttt ctggcgtttt cggggcggct tcgggataag ggtcgattcg ggactcgaac
    31201 caggaaaata ttttgttcaa cattgttttc gatttcggta aaacagtttc agacggcatc
    31261 aaacacaatg ccgtctgaaa ggaaggacaa taacgccatt ttacgggaaa agccgtcggg
    31321 aagacagcgc gaggcggaaa cgcagggttt cgtcagggca aacgccgcgc cgccttcaga
    31381 cggcattatt tcagcaggtt tttcaaagca aggcgcacgc cttcgcccac gtccgtcccc
    31441 tccggaacgc ctttgaccgc cgcttttgct tcgcgttcgc tgtaacccag cgcaagcagc
    31501 gtgctgacga tgtcttccgt ttcgtcggcg gcgggtgcgg cggcaaacag cccgtccgtt
    31561 accgcatgcg cgaccagctt gccgcgcagt tccaagacca tacgttcggc ggtttttttg
    31621 ccgattcccg gcgcggaaga caggcgtttg acatcttctt cggcaaccgc ccgcgccagc
    31681 tcgtctgccg tcatcgctga caaaatgccc aaagccgttt tcgcgccgat gccgccgacc
    31741 ttaatcagtt ggcggaaggt cttgcgttct tccgcagtgg caaaaccaaa taaaagatgt
    31801 gcgtcttccc gaatgataag ctgggtaaac agttgtacgc tttcacccac gggcggcagg
    31861 ttgtagaagg tctgcatcga tacgtcggcc tcatagccga caccgttgac atcgatgacg
    31921 atttgcggag ggtttttttc aaccagtttg ccggtcagtc tgctgatcat gtatgccgaa
    31981 tcctgaagtg tcgggtgcaa aatgccgtct gaaaccggtt cgggcttcag acggcacgga
    32041 ttgtatcaaa ttcagtcgtc gcggcgggag gaaatcacgc ggccggtacg ggcatcgaca
    32101 acgactttgt attcctgtcc gtttttgacg atttcgacat catagtgcgg acggccgttg
    32161 tcgtgttcga gatcgatgtc ggtgattttg ccgccgacac gcgccaacgc tgctttttcg
    32221 gcttgggcgc ggctgatgat tttgtcttgt ttgctgtgtt ggtgtgcggc gtgtccgtgg
    32281 tcgtcatcgc cgtgtccgtc gtggtgggcg agtgcggggg cggaaatgcc cagcagtgcg
    32341 gttgcggcgg cggtcaagag aaggtgtttg atgttcatat tttgcctttg taaatcgtgg
    32401 gttggaaaat gtggatatta ataaggtatc aaataaccgt cagccggcag tcaataccgc
    32461 ccgaaccata ccgtgcgcct gagcttcggc ctcggcggcg cgttcctgcg aggcaaacgg
    32521 acccattttg acgacgtatt cgtaacggcg tttttcaatg gcgaggttcg ggcttgatgt
    32581 cgaagcggcg aagttttggg cggcttggtt cagataggct tgtgcttcgt gttccgtacc
    32641 gaaagatttc aagtcgataa agatgtcttt gttttcggca accggtgcgg atttgcccgg
    32701 gacgatttgt tcgattttga cgtgcgccgt cccttggctg acaaagccca atttttgcgc
    32761 ggcggctttg gatacgtcga tgatgcggtt gccgtggaag gggccgcggt cgttgacgcg
    32821 gacgatgacg cttttgccgt ttttggtatt ggttacgcgc acatagctgg ggatgggcag
    32881 ggttttgtgg gcggcggtaa aggcgttcat atcgtatcgt tctccgccgg aagttttgcg
    32941 cccgtgaaac ctgccgccgt accacgaggc gttgccggtt tgcgtgaatt cggcgacttg
    33001 gtttttcggc gtgtagcgtt ttccggcgac tttgtagctg cggttggcgg aggcgtgcag
    33061 tttttctgcc ttgaccactg cgtcggcgga tgccgtctga agggagtgtg tgccgaatgc
    33121 ggcggtgaga aggaaaaggg tttttcgggt taaagtcaaa acgtgttccg ttcttgagtt
    33181 gaagacgaat gggcatcatg cccgccggat acgttccgaa ccgccgtaca gtgcggacgg
    33241 cggttcggaa tgtgtccgga taggttttca gacggcatga acctgcgttc aaacgccgcc
    33301 tgcgtaaccg tgttgccgcc acgcttcaaa gagaatcacg gcaacggtat tggaaaggtt
    33361 catactccgg ctgccgggct gcatgggcag gcggattttt tgcgcggcgg gcaggctgtc
    33421 gaggatgtcg gcaggcagtc cgcgcgtttc cggcccgaac agtaaaacgt cgcctttttg
    33481 aaacgcggtt tcatcggggc gcgccgtgcc tttggtggtc agggcgaaaa tgcgcctgcc
    33541 tgcgagtgcc ttgaggcagt cgtcgaagtt ttcgtacacc gtcaggctgg cgaattcgtg
    33601 gtagtcgagc ccggcgcgtt tcattttggc ggaatccaat gggaagccga gcggtttgac
    33661 aaggtgcaaa tccgcgccgg tattggcgca caggcggatg atgttgcccg tgttcggcgg
    33721 gatttccggt tggtataaaa cgatggtaaa cataaatatc aatcacttat aggcgcgtaa
    33781 ccttgccaca aggcggatgg ggtgtcaaaa aatttagtta ttttttcatt ggcgtgcgtg
    33841 ccagcgtcca gcagcagatg cggtttgcgc ctaatttttt cagcgtcttt gccaattcgt
    33901 ccagcgtcgc gccggtggta aagacatcgt cgattaacag aatattacag ttttccggta
    33961 tcggtgtgcg gatttcaaag gcgtttttga tgtttcgccg ccgttcgccg cctttgagcg
    34021 tgctttgcgg cgggcggtgg tgtcggaaaa cggtgtgtcg gggcagtatc tgccagccgt
    34081 agcgttgtgc cagcagcccg acgatgcttt cactttggtt gaacccgcgt tgcagcagcc
    34141 gctccctgct tagcggtacg ggcaggacga aatcgaaaca ttcgtctgca agccggtcgg
    34201 gcggattctg catcatcagg tctgccagcg gctgcaccat gctcaaatca gccaagtgct
    34261 tcagcgcgtg tatcatattg ctgacgggcg gttcgtaatg cagcgaagcc cacatccggt
    34321 cgaatgcggg cggttttttc tgacagccgc cgcacaccgc gccgccttgg atgtgtctga
    34381 aacacagggg gcagctgttt gccgcgtcgg tgcggtatgc ccccaaatcc gccgcgcagc
    34441 cggcgcagat gccgtctgaa acgccggacg aaccgtggca taatacgcaa cgcctgatag
    34501 tgggcgcgtc tgcgatgcgc cgccaacaag agagaaaatt catgcctgat gctgccaaaa
    34561 aagtttacct gatacacggg tggggggcga accgccacgt gttcgacgat ttgatgccgc
    34621 gcctgcctgc aacgtggccg gtgtccgccg tcgatttgcc cggacacggc gacgcgccct
    34681 tcgcgcaacc cttcgatatt gaggcagccg ccgacgctgt tgccgcacaa atcgatacgc
    34741 ccgccgacat tctcggctgg tcgctcggcg gattggtcgc gctgtatctg gcggcgcgcc
    34801 atcccgacaa agtccgttcg ctctgcctga cggcgagttt cgcccggctg acggctgccg
    34861 aagattatcc cgaagggctt accgcgcccg cattgggcaa aatggttggc gcgttccgaa
    34921 cggattatgc caaacatatc aaacagtttc tacaattgca gcttctgcac acgcctgatg
    34981 cggacagaat cataggcaga atcctgcccg atttggcgcg ctgcggcacg ccctccgcct
    35041 tacaggaagc tttggacgcg gcggaaaggg cggatgcgcg gcatttgttg gacaagatag
    35101 atgttccggt actgctggtg ttcggcggca aagacgcgat tacgccgccg cgcatgggcg
    35161 agtatctgca ccggcatttg aaaggaagca ggctggttgt gatggaaaag gcggcacacg
    35221 cgccgttttt gagccacgcg gaagcgtttg ccgcgctgta ccgcgacttt gttgaagggg
    35281 tttgagatga atccgcagga caaatgctgg caggttcacc gccatcttgc cgaacatacc
    35341 gaccaacggc tgacactcgt ccgcaacgcg cccaagcata tcctgcttgc cggtgcggat
    35401 gcggacatca gccgcagcct gctggcgaaa cgctatccgc aggcggtatt tgaagaatac
    35461 gattcccgtg cggatttttt ggcggctgcc gctgccgccc gcaaaggcgg tttttggcaa
    35521 aggtttacgg gtaagggcgt ggtgcaacac tgccaatccc cgatcgcgcc gctgcccgaa
    35581 gcgtgtgccg atatgttgtg gtcgaatctc ggactgttgg cggcggaaca aatccttcct
    35641 gtgctgcaca actgggcgcg cgccttgaag acggacgggc tgctgttttt tacctgcttc
    35701 gggcgagata ccttggcgga actgaaatgc cgtctgaaag aaaacggcat tgaaagccgc
    35761 agcgcgcttt tccctgatat gcacgacttg ggcgatatgc ttgctgaaaa cggcttttac
    35821 gaccccgtta ccgatacggc gaagctggtg ttggattaca aaaaggcgga aacgttttgg
    35881 gcggatatgg acacgctggg cgtttggcgg gcgatggcgt ggaacgatga aaacgccgcg
    35941 cgttcgtgtg tcgggacaat atttgagcgg gaaggcggtt tgggcattac gctggaaacg
    36001 gtgtacggac acgccgtgaa aaaactgatg ctgccgcaag gggagaacgt ggtgcagttt
    36061 tttccgaaga gatgatgtgc agatgccgtc tgaagccgtt tccaagtttc agacggcatt
    36121 tgtttgtgaa aaccgacaga actaaaggaa atgccgatgt atagtgaatt a
//
LOCUS       DAWHRS010000004        34162 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWHRS010000004 DAWHRS010000000
VERSION     DAWHRS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.004"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            142..1110
                     /locus_tag="WIB82_000134"
     CDS             142..1110
                     /locus_tag="WIB82_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ3841512.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMMLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDILGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            1100..2074
                     /locus_tag="WIB82_000135"
     CDS             1100..2074
                     /locus_tag="WIB82_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ3841513.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTLGGV
                     GYASLPLTGKFGFELIVMMGGSLLLFYTLIKQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHNELLGIGALILLISAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2119..2745
                     /locus_tag="WIB82_000136"
     CDS             2119..2745
                     /locus_tag="WIB82_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841514.1"
                     /translation="MTQERLPDFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGERDEGTTGVTASVVQLLTS
                     AAPETGFGGVGPAGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVSGSASAEELEHFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            2828..3586
                     /locus_tag="WIB82_000137"
     CDS             2828..3586
                     /locus_tag="WIB82_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3841515.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKRTTVAVLHDINQAAAYADHVVAMKNGKIALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(3645..3983)
                     /locus_tag="WIB82_000138"
     CDS             complement(3645..3983)
                     /locus_tag="WIB82_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ3841516.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            4078..4290
                     /locus_tag="WIB82_000139"
     CDS             4078..4290
                     /locus_tag="WIB82_000139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841517.1"
                     /translation="MPETVIPAQVGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            4447..8409
                     /gene="purL"
                     /locus_tag="WIB82_000140"
     CDS             4447..8409
                     /gene="purL"
                     /locus_tag="WIB82_000140"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ3841518.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKRQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPIDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIVAANIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVEDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG
                     "
     gene            8504..9262
                     /gene="gloB"
                     /locus_tag="WIB82_000141"
     CDS             8504..9262
                     /gene="gloB"
                     /locus_tag="WIB82_000141"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ3841519.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            9569..13863
                     /gene="mspA"
                     /locus_tag="WIB82_000142"
                     /gene_synonym="ausI"
                     /pseudo
     CDS             9569..13863
                     /gene="mspA"
                     /locus_tag="WIB82_000142"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
     gene            14095..15549
                     /gene="mgtE"
                     /locus_tag="WIB82_000143"
     CDS             14095..15549
                     /gene="mgtE"
                     /locus_tag="WIB82_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ3841520.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(15635..16543)
                     /gene="hslO"
                     /locus_tag="WIB82_000144"
     CDS             complement(15635..16543)
                     /gene="hslO"
                     /locus_tag="WIB82_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ3841521.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            16788..17543
                     /locus_tag="WIB82_000145"
     CDS             16788..17543
                     /locus_tag="WIB82_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ3841522.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            17562..17780
                     /locus_tag="WIB82_000146"
     CDS             17562..17780
                     /locus_tag="WIB82_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841523.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            17925..18272
                     /locus_tag="WIB82_000147"
     CDS             17925..18272
                     /locus_tag="WIB82_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ3841524.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRGIIR"
     gene            18269..18961
                     /locus_tag="WIB82_000148"
     CDS             18269..18961
                     /locus_tag="WIB82_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ3841525.1"
                     /translation="MNEILRQPSVLLFLTLAVYAIAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            19028..20248
                     /gene="argJ"
                     /locus_tag="WIB82_000149"
     CDS             19028..20248
                     /gene="argJ"
                     /locus_tag="WIB82_000149"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ3841526.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            20308..21724
                     /locus_tag="WIB82_000150"
                     /pseudo
     CDS             20308..21724
                     /locus_tag="WIB82_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            21914..23305
                     /gene="hrpA"
                     /locus_tag="WIB82_000151"
     CDS             21914..23305
                     /gene="hrpA"
                     /locus_tag="WIB82_000151"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ3841527.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKAIAKNQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMAALKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            23449..25092
                     /locus_tag="WIB82_000152"
     CDS             23449..25092
                     /locus_tag="WIB82_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3841528.1"
                     /translation="MDEIQIPKKVELQTKLENEKIVLSKGSTTIIVGANGTGKTRLAV
                     YIEEQLKEKAHRISAHRALKLNPNVNKIPEKSAKTYLSYGQNWDGIDVSNRKNYRWDN
                     NSYTHLLNDFDWLLQYLFAQQNNIAVANNQKLNRNEKVTNSKTKLDILQEAWETLLPH
                     RKLHITADDIQVSAVDNEELYSASNMSDGERALFYILGQVLSVDDGSVLIFDEPELHI
                     HKSIISNLWDKIEELRPDCSFLIITHDIEFAATRVAKKYVIRNYYPTPAWDISEVPES
                     NFDEETITMILGSRKPILFVEGNNNSLDIATYRYCYPDWTIIPKGACKDVIQSVSSLK
                     KLSNEMPLLNLKCSGIVDLDSRDEREIEQLNNLGIYILPVSEIENLFSLTDVAKEILK
                     LNQYSDEELLNKLNGFKSELIKYIDNELKDDKLDEFVVKQVRRKIDNYLKNIDLSSKI
                     TSTDMKKSLLNEISTLTEQKIETWISEIKNEIQRCIEQQDLDKLLTIYDNKGLLAKSA
                     CVLKGMRNKHEFESWIMRTLKGRNKDFIDAIRQKLPILD"
     gene            25136..25318
                     /locus_tag="WIB82_000153"
     CDS             25136..25318
                     /locus_tag="WIB82_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841529.1"
                     /translation="MKNHQTTLSFPTSSSLKPCADKQGLSEDRSQYRLTKLGEQMAHL
                     PIDPKIARILLVLFRF"
     gene            25530..27107
                     /gene="lpt3"
                     /locus_tag="WIB82_000154"
     CDS             25530..27107
                     /gene="lpt3"
                     /locus_tag="WIB82_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ3841530.1"
                     /translation="MMKKSFLTLVLYSSLLTASEIAYRFVFGIETLPAAKIAETFALT
                     FVIAALYLFARYKVTRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPVLWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSRIPAFKQPAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNAIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENEMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGKHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            27209..30592
                     /locus_tag="WIB82_000155"
     CDS             27209..30592
                     /locus_tag="WIB82_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ3841531.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQIARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDASAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEVRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYAAQQTTPSPVGEGRGEGKTVAAQTNFSATAANPLPNPLP
                     QEREQSAAASTISDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATSTNPLPQERE
                     QSASASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATSTLSDDSKPKKQPA
                     PQKNRLKPLPLADIRTFQAWLKTAERDNLRLLFLSRDDLMQHAAAHITEEQFPKFWQT
                     ADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMIREKIQLQIKALPK
                     QIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRIFEQINQDEWAAFRL
                     PEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDNVTAWDIGT
                     LPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQ
                     VKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKR
                     ARSRLPAVKEALSRYLQETAAVYAELNSKLGKHPLTHLLRLRLQTLLAAGFATRTPWA
                     QWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDSLIKQGLPISDGL
                     AAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEKIEK"
     gene            complement(30625..31716)
                     /locus_tag="WIB82_000156"
     CDS             complement(30625..31716)
                     /locus_tag="WIB82_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ3841532.1"
                     /translation="MQTFNRERLEFALDKRGCTQTKLAELLGITARQVRNYVKDEQIP
                     DLNELANLLEFPVHFFTNDERLPELKSQAVSFRARSRTSKRLEKQALNHSITAFLLND
                     WLETEFTLSQAVLPDFSDISPEEAAQELRLEWGLGNEPIPNMITLLEAKGVRVFSLSL
                     DTKDIDAFCTWYENHPFVFLNTQKSAERSRFDAAHELGHLIRDKYSMEHSKNSEITED
                     EPRDIIEKEANAFASAFLMPEAALRLYRHVPITIENLLKIKRRFGVSLVALAYRMHKL
                     GMITDRMYIHNLCPLFAKKKYRTIEPEPMEREISSALRKMLTILKNDGIGIEQIAENL
                     NVSPKDVADLTFGLVERDLNRYRKLRLVK"
     gene            complement(31732..32376)
                     /locus_tag="WIB82_000157"
     CDS             complement(31732..32376)
                     /locus_tag="WIB82_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841533.1"
                     /translation="MVEFALSEPFPFEVGEIVEQQNSDIALSEISKYLSQEMLKEIVL
                     TAHNNIQDKAALSDRNTLGMYRQAEITFALRVYLSKDGWTLEPWSGGAFLCLSPCRTK
                     SIIVATATSDVGRHHGMPMTTARKGKGFEEAIRNSKLDLPDTVQFWVLLFHWKYNKVQ
                     LELSLPSGFVSHQITGYKQRIILADVDLTKFDELERKQPVEEYEPLVERKQKAG"
     gene            32733..32885
                     /locus_tag="WIB82_000158"
     CDS             32733..32885
                     /locus_tag="WIB82_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841534.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWV"
     gene            33255..33410
                     /locus_tag="WIB82_000159"
     CDS             33255..33410
                     /locus_tag="WIB82_000159"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ3841535.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(33500..33949)
                     /locus_tag="WIB82_000160"
     CDS             complement(33500..33949)
                     /locus_tag="WIB82_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ3841536.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
     gene            complement(34037..>34162)
                     /locus_tag="WIB82_000161"
     CDS             complement(34037..>34162)
                     /locus_tag="WIB82_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEZ3841537.1"
                     /translation="DDLTKVKGIGPAVLAKLKDQASAGAPAPKGPAKPAIPAAKK"
BASE COUNT         8712 a         9920 c         8112 g         7418 t
ORIGIN      
        1 gcgggaatgg cggcaagcgt ttttattgga acttgcccta tcctctgccc gatacggagg
       61 cttcaaacaa ggctttccgc tccgacggtt cggactgcct tgtttgaatc ttctacgcct
      121 taacgctttt cccttctgtt tatgactgcc aaaccttttt ccctcaacct gaccaacctc
      181 ctgctgctgg cggtgttgtt tgccgtcagc ctgtcggtcg gcgttgccga tttccgctgg
      241 tcggatgtgt tttcgctgtc ggacagccag caggttatgt tcatcagccg cctgccgcgc
      301 acgtttgcga ttgtgctgac gggcgcgtcg atggcggtgg cggggatgat tatgcagatt
      361 ctgatgcgta accgttttgt cgaaccttct atggcgggcg cgggtcagag tgcggctttg
      421 ggtttgcttc tgatgtccct gctgctgcct gccgcgccgc tgccggtcaa aatgtcggtt
      481 gccgccgttg ccgcgttaat cgggatgctg gtgtttatga tgcttatccg ccgtctgccg
      541 ccgaccgcgc aactgatggt gccgctggtc gggattattt tcggcggcgt ggttgaggcg
      601 gtggcgacct ttatcgcgta tgaaaacgaa atgctgcaaa tgctcggcgt gtggcagcag
      661 ggcgattttt cgagcgtgct gctggggcgg tacgagctgc tttggattac gggcggtttg
      721 gcggtgtttg cctatctgat tgccgaccgg ctgacgattt tggggctggg cgagacggtc
      781 agcgtgaatt tgggtttgaa ccggacggcg gtgttgtggt cggggctgat tattgtggct
      841 ttgattacgt cgctggttat cgttacggtc ggcaatattc cgtttgtcgg gctggtcgtg
      901 ccgaacatca tcagccgcct gataggcgac aggctgcgcc aaagcctgcc tgcggtggcc
      961 ttgttgggcg cgtctttggt gttgctgtgc gatattttgg gacgcgtgat tgtgtttccg
     1021 tttgaaattc cggtctctac ggtttttggt gtgttgggta cggctttgtt tttgtggctt
     1081 ttgttaagga aacctgctca tgccgtctga aaaaaatatc ggttttatgg caggaagcag
     1141 ccgtccgttg tgggttgcct ttgcgctgtt gctggtttcc tgcatcctgt ttatgacgct
     1201 caacgtcaaa ggcgattggg actttgtact gcacctgcgt ctgaccaaac ttgccgcgct
     1261 gctgatggtc gcctatgcgg tcggcgtgtc cacgcaactc ttccaaacgc tgaccaacaa
     1321 tccgattctg accccttcaa ttttgggttt cgattcgctg tatgtgtttt tgcagacctt
     1381 gctggtgttt acgctcggcg gcgtgggcta tgcttccctg ccgttgacgg gcaaattcgg
     1441 ctttgaactg atcgtcatga tgggcggctc gctgctattg ttctacacgc tcatcaaaca
     1501 gggcggacgc gatttgccgc gtatgatttt aatcggcgtg attttcggga ttttgttccg
     1561 cagcctgtca tccctgcttt cgcgcatgat agaccccgaa gaatttaccg cggcgcaggc
     1621 gaatatgttt gccggattca ataccgtcca caacgaactg ctggggatag gcgcgctgat
     1681 tctgctcatc agcgcggcgg cggtttggcg cgaacgctat cgcttggacg tacacctttt
     1741 ggggcgcgac caagccgtca atttgggcat cagctacacg cgcaacacct tatggatact
     1801 gctttggatt gccgcattgg tggcgacggc gaccgccgtg gtcggcccgg taagcttttt
     1861 cgggcttctc gccgcctcgc ttgccaacca cttttccccg tccgtgcgcc attccgtccg
     1921 cctgccgatg acggtttgtg tcggcggtat cctcctggtc ggcggacaaa ccgtattcga
     1981 acacttcttg ggcatgaagg cggtattaag cgtggtggtc gaatttgcgg gcggactcgt
     2041 tttcctctat ctcgttttaa aacacaaaaa atgacggatg ccgtctgaac ggcagcccgc
     2101 cccgaaagga caaaccatat gacgcaagaa cgtttacccg attttttcga ccgcgccccg
     2161 acgctgaccg tacaagaccc gcttgccgca ttcctcggcg cggccgaaaa cggcatcctc
     2221 acttaccgct acgccgatgc cgtgcgcctg tgcggacatt cctgcccgac cgtcgcgggc
     2281 gcgtacctga tggttatcaa aggtctgaaa gccctttacg gcgaagagct gcccgaacgc
     2341 ggcggcatcg aagcctttat gcagggtgag cgagacgaag gcacgaccgg cgtaaccgcg
     2401 tccgtcgtcc aactcctcac cagcgcagcc cccgaaaccg gctttggcgg cgtaggcccg
     2461 gccggacgct tcgcccgccg caacctctta tcctttggtg caggcgaaat caacggcaca
     2521 ctgaccctgc gccgcaaaga caacggcaaa accgtcgccg tcagcctcaa cgccgccctg
     2581 caacccttcg caccgcaaat gcgcgacatc atgcccaaag ccgtcagcgg cagcgcaagc
     2641 gcagaagaac tcgaacactt cggacaactc tggcaggcac gcgttaaagc atttttgacc
     2701 gaatcggcgg acgacccgca gttcgtcatc gtccgcgaag tgtgagcgtt cagacggcat
     2761 tccgaatttc aaatgccgtc tgaaccccgc caaacaacaa caaacctacg cccgacaagc
     2821 atccgccatg attaccatcc gcaacgtcag ctaccgcatc ggcacacgcc ccatcctcga
     2881 caacgtcagc ctcgacatcc ccgaaggcgg cattaccgcc ctcgtcggcc ccaacggcgc
     2941 gggcaaatcc accctgtttt cctttatggc gcggctgcgc ccgcttgaaa gcggcagcat
     3001 cgcctaccgc ggcaaaaacc ttgccgacac ccccaccgcc gaactcgccc gcacgctctc
     3061 catcctcacc caagaaaaca gcatcatgag ccgcattacc gtgcgcgacc tgctgatgtt
     3121 cggccgttac ccctaccatc aaggcagacc gactgccgaa tgccgccgta tcgttaacgg
     3181 tgcaatcgaa gaattccacc tgcaagacct ctccgaccgc tatctgaccg agctctccgg
     3241 cggacaacgc caacgcgcca tgattgcgat ggtgttctgc caaagcaccg actacgtcct
     3301 tttggacgaa ccgctgaaca acctcgatat gtatcacgcc cgctcgctca tgcagattct
     3361 gcaaaagctc acgcgcgaac acaaacgcac caccgtcgcc gtattgcacg acatcaacca
     3421 ggccgccgcc tacgccgacc acgtcgtcgc catgaaaaac ggcaaaatcg ccctgaccgg
     3481 cacgcccgaa gaagtgttca ccgtacacaa catcaaagaa ctgttcgaca tggacgtgga
     3541 cgtactcgac tacgaaggca aaaaactcat cgtccaccat atctgaagct caaaaagaaa
     3601 atgccgtctg aaaacctttc agacggcatt tccattccaa acgctcaaac cgccgcgtcc
     3661 gcacgttcgc ccgtgcggat acggattgcc tcctcgaccg gcagcacaaa aatcttgccg
     3721 tcgccgattt ttcccgaacg cgccacctcg acaatcacgt caatcgcgcg ttccacagca
     3781 tcatccgcca acaccagctc gattttgacc ttgggcagga aatcgacggc gtattccgcg
     3841 ccgcgataga tttccgtatg ccccttctgt ctgccgaacc ctttgacctc gctgacggtc
     3901 atgcccgtaa tgccgatttc cgtcaacgcc tcgcgcacgt cgtcgagttt gaacggtttg
     3961 acaatcgcct cgattttttt catcacattc tccatgaatt aacgtaaaca aacgacaaat
     4021 atagcagaaa ccgccggcaa acgataaacg gtactgttcg cccggcgatg cggcgatttg
     4081 ccggaaaccg tcattcccgc gcaggtggga atcccgatct ctgcatttca gcaatattta
     4141 aaggtttctg taaatccaaa tttccatatt cctaaccgtg cggaaacgac ggcatttgat
     4201 ctaaaaacat acggaagcag ttgcccgccc ctctacttcc tgcaattatc cgcatacccg
     4261 gctttgggta aaaatcagaa aaacagataa aacccttatt ccttaatatc ttcagacggc
     4321 acctgtattt tccccaactg tatgccgtct gaagattgtc aacattttaa gccaaaatac
     4381 ccaagcaata cagccccgtt gcgcgtataa tgacagattt tccaaccgca tttgagagcc
     4441 gaatccatgt ctgtcgtttt gcccttgcgc ggcgttaccg ccctttccga tttccgtgtc
     4501 gaaaaactct tgcaaaaagc cgccgcactc ggactgcccg aagtcaaatt aagcagcgaa
     4561 ttttggtatt tcgccggcag cgagaaagca cttgatgccg cgactgtcga aaaactgcaa
     4621 gccttgttgg cggcgcaaag cgttgaacaa acgccaaaag cgcgcgaggg cttgcatttg
     4681 tttttggtaa cgccccgttt gggtacgatt tcgccgtggg cttccaaggc gaccaatatc
     4741 gcggaaaact gcggtttggc aggcatcgag cgcatcgagc gcggcatggc ggtgtggctg
     4801 gaaggtgcgc ttaccgatga acagaaacgg caatgggcgg cgttgctgca cgaccgcatg
     4861 accgaatcgg tgctgcccga ttttcagacg gcatccaaat tattccacca tctcgaatcc
     4921 gaaacctttt ccaccgttga tgttttaggc ggcggtaaag aggctttggt caaagccaat
     4981 accgaaatgg gtttggcact ctccgccgac gaaatcgatt atctggttga aaactatcag
     5041 gctttgcagc gcaatccgtc cgatgtggaa ctgatgatgt tcgcgcaggc aaacagcgaa
     5101 cactgccgcc acaaaatctt caacgccgat ttcatcctca acggcgaaaa acaaccgaaa
     5161 tccctgttcg gcatgatacg cgacacgcac aacgcgcatc ccgaaggtac ggtcgtcgcc
     5221 tataaagaca attcgtccgt gatcgaaggc gcgaaaatcg agcgtttcta tccgaatgcg
     5281 gcggaaaacc aaggctaccg tttccacgag gaagacacgc atatcatcat gaaagtggaa
     5341 acgcacaacc acccgaccgc catcgcgccg tttgcaggcg cggcaacggg agcgggcggc
     5401 gaaatccgcg acgaaggcgc aacgggcaaa ggttcgcgtc cgaaagcagg cttgaccggc
     5461 tttaccgtgt ccaacctcaa catccccggc ctcaaacagc cgtgggaaca agattacggc
     5521 aagccggaac acatttcctc gccgctggac atcatgatcg aaggtccgat cggcggcgcg
     5581 gcgttcaaca acgaattcgg ccgccccaac ctcttgggct acttccgcac ttttgaagag
     5641 aagtttgacg gtcaggttcg cggctaccac aaaccgatta tgattgccgg cggcttgggc
     5701 agcattcagg cgcagcagac gcataaagac gaaatccccg aaggcgcgtt gctgatccaa
     5761 ctgggcggcc cgggcatgct tatcggcttg ggcggcggcg cggcttcttc gatggatacc
     5821 ggcacgaacg acgcttcttt agacttcaac tccgtacaac gcggcaaccc cgaaatcgaa
     5881 cgccgcgcgc aggaagtcat cgaccgctgc tggcagctcg gcggcaaaaa cccgattatc
     5941 tccatccacg acgtgggcgc aggaggtttg tccaacgcct tccccgaact ggtcaacgat
     6001 gccggacgcg gcgcggtatt cgagctgcgc gaagtgccgt tggaagaaca cggtttaacg
     6061 cctttgcaaa tctggtgcaa cgaatcgcaa gagcgttatg tcttgtcgat tttggaaaaa
     6121 gatttggata ccttccgcgc catctgcgaa cgcgaacgct gcccgtttgc cgtggtcggt
     6181 acagcgaccg acgacggtca tttgaaagtg cgcgacgatt tgttcgccaa caaccccatc
     6241 gatttgccgt tgaacgtctt gctcggcaaa ccgcccaaaa ccacgcgtac cgacaaaaca
     6301 gttacgccat ctaaaaaacc gtttcacgcc gacgatatcg acatcaccga agccgcctac
     6361 cgcgttttgc gcctgcctgc cgtagccgcc aaaaacttcc tgattaccat cggcgaccgc
     6421 agcgtcggcg gcatgaccca ccgcgaccaa atggtcggca aataccaaac ccccgtagcc
     6481 gactgcgccg ttaccatgat gggcttcaat acctatcgcg gcgaagcaat gtctatgggc
     6541 gaaaaaccgg ccgtcgccct gtttgacgcg cctgcctcgg gcagaatgtg cgtcggcgaa
     6601 gccatcacca acatcgtggc agccaatatc ggcgacatcg gcaacatcaa actctccgcc
     6661 aactggatgg cggcgtgcgg caacgaaggc gaagacgaaa aactctaccg caccgtcgaa
     6721 gcggtttcca aagcctgtca ggcattggat ttgagcattc ccgtaggcaa agacagcctg
     6781 tcgatgaaaa ccgtatggca ggacggcgaa gagaaaaaat ccgtcgtttc gccgttgagc
     6841 ctgattatct ccgcgtttgc gccggtggaa gacgtacgca aaaccgttac gcccgaactg
     6901 aaaaacgtcg aagacagcgt attgctgttt atcgatttgg gcttcggcaa agcgcgtatg
     6961 ggcggctcgg cgtttggtca ggtgtacaac aatatgagcg gcgacgcgcc cgatttggac
     7021 gatacaagtc gtctgaaagc gttttacaac gtgattcagc agcttgtcgc cgaagacaaa
     7081 ctcttggcgt atcacgaccg cagcgacggc ggtttgtttg ccgttttggt agaaatggcg
     7141 tttgcggggc ggtgcggctt ggatatagat ttaaatttat tgcttgcaca aacatttatt
     7201 accaaccata ccgctctgtc tcaatcattg cggactgaag aggtaaaagc gttggctgaa
     7261 tggcaagaaa ccattgcccg cacattattt aatgaagagt tgggtgctgt tatccaagtt
     7321 agaaaacaag atgttgccga tattatcaat ttattctatc aacaacagct gcatcataat
     7381 gtctttgaaa tcggtacgtt aactgatgag aacacgttaa tcatccgcga cgggcaaacg
     7441 caccttattt ctgacaacct aatcaaactg caacaaacct ggcaagaaac cagccatcaa
     7501 atccaacgct tgcgcgacaa tcctgcctgc gccgacagcg agttcgcact gattggcgac
     7561 aacgaacgca gcgcattgtt tgccgacgtg aagttcgacg tgaacgaaga catcgccgcg
     7621 ccgtttatca acagcggcgc gaaacccaaa atcgccatcc tgcgcgaaca gggcgtaaac
     7681 gggcaaatcg aaatggccgc cgcgttcacc cgtgccggat tcgatgccta cgacgtgcat
     7741 atgtccgacc tgatggcagg ccgcttccgc cttgccgact tcaaaatgct ggcggcgtgc
     7801 ggcggcttca gctacggcga cgtactcggc gcgggcgaag gctgggcgaa atccatcctg
     7861 ttccaccctg ctctgcgcga ccagtttgcc gccttcttcg ccgacccgga cacgctgaca
     7921 ttgggcgtgt gcaacggctg ccaaatggtc agcaaccttg ccgaaatcat ccccggcacg
     7981 gcaggctggc cgaagttcaa acgcaacctg agcgaacagt ttgaagcgcg cctgagcatg
     8041 gttcatgtcc ccaaatccgc gtcgctgatt ctgaacgaaa tgcaaggctc cagcctgccc
     8101 gtcgtcgtca gccacggcga aggccgcgcc gacttcgcgc ttcacggcgg caatatttcc
     8161 gccgatttgg gcatcgcgct gcaatatgtg gacggtcaaa accaagttac ccaaacctat
     8221 ccgctcaacc ccaacggctc gccgcaaggc atcgccggca ttaccaacgc cgacggccgc
     8281 gtgaccatca tgatgcccca ccccgaacgc gtgtaccgcg ccgcacaaat gagctggaaa
     8341 ccggaaggct ggacggaact gtccggctgg taccgcctct ttgccggcgc acgtaaagcc
     8401 ttgggctaac cgccctactc aaaccaatgc cgtctgaaga atatttcaga cggcgttccg
     8461 gcataccatc ctttaaacgg tatccgtcca ccgaggaaca ctcatgaaaa tcacacccgt
     8521 caaagcccta accgacaact acatctggat gatacagcac ggcaaccatg ccgtctgcgt
     8581 cgacccttcc gaaccctcgc ccgtcttgga attcctcgtc cgcaaccgcc tcatgcttgc
     8641 ccaaacatgg gtaactcacc cccatcccga ccacgagggc ggtgcggcgg cactctggcg
     8701 cggctacatg gaatcgcccg tttacggcga atccgacatc gaagccgcca cccacaccgt
     8761 aaccgcaggc acccaattca ccttcggcga cggacaggtt accgtttggg caacacccgg
     8821 ccacacagac cgccacacca gctaccttct cgaaacttca gacggcatac acgtcttttg
     8881 cggcgacacc cttttttccg ccggctgcgg acgcgtgttt accggcacaa tcgaacagct
     8941 ttacgacagc ttccaacgct tcaaccgcct gcctgaaaac accctgttct atccggcgca
     9001 cgaatacacc gccgccaacc tggattttgc cgcccatatc gagccggaca acgccgacat
     9061 tcagacggca ctgaaagcgg cggcgcatac gcctaccctg cccgttaccc tcgcgcacga
     9121 acgccgtgtc aatccgtttt tgcgcgtcga cctgccgcac gtcagagacc gcgccgaggc
     9181 attgagcggg aaaacgttaa acagcagcct cgataccttt gtcgcgctgc gtgaacttaa
     9241 aaaccaatac cggacgaaat aaaacaacgg gaaaacgcag ccattcctag gatttttatt
     9301 aaaatcttaa ataaaatcat acaatcatcg ccaatagacg aaaggacacc gttgccttat
     9361 aatcaaacaa aaacaaaata tataatatag tggattgaat ttaaatcagg acaaggcgac
     9421 gaagccgcag acaatacaga tagtacggca aggcgaggca acgctgtact ggtttttgtt
     9481 aatccactat ataaatccag cacaaaacgg gatcggtgat tcttgtccgc aagaatcgtt
     9541 gattttctct attacacgga taatcatcat gcgcttcaca cataccatcc cattttattc
     9601 cgtattgtcc accctcggtc tttttgccgt ttcccctgct tactcatcca ttgtccgcag
     9661 cgatgtcgat taccaatatt ttcgcgactt tgccgaaaat aaaggcgcgt tcgccgtagg
     9721 tgcaagcaat atttccatcc aagacaagca aggcaaaata ttaggcaggg ttctcagcgg
     9781 catccccatg cccgacttcc gcgtcagcaa ccggcaaacc gctatcgcca ccctggttca
     9841 cccccaatac gtcaacagtg tcaaacacaa cgtcggctac ggttccatac aattcggcaa
     9901 cgacacccaa aaccctgaag aacaagccta tacctaccgc ctcgtatcac gcaacccgca
     9961 cccggactac gactaccacc ttccccgcct caacaaactg gttaccgaaa tctcacctgc
    10021 cgcactcagc agcgtaccct tgcttggaaa cggccagcca aaggccaatg cctacctcga
    10081 taccgaccgc ttcccatact ttgtacgact cggctcaggc acgcaacaag tccgcaaagc
    10141 agacggcacg cgcacacaaa ccgccccggc ataccaatac ctgaccggcg gcacgccgct
    10201 gaaagtattg ggtttccaaa accacggctt actcgtcggc gacagcctga ccgaccaacc
    10261 ccttaacacc tacgcagtcg ccggagacag cggttccccc ctgttcgcct tcgacaagca
    10321 tgaaaaccgc tgggtgcttg cgggcgtact cagcacctac gccggcttcg acagcttctt
    10381 caacaaatac atcgtcacgc aacccgaatt catccgttcc accatccgcc aatacgaaac
    10441 ccggctggat gtcgggctga ccaccaacga actcatatgg cgcgacaacg gtaatggcga
    10501 cagcaccctg caagggctca acgaacgcat caccctgccc attgcaaacc cttcgcttgc
    10561 cccacaaaac gacagcaggc acatgccgtc tgaagatgcc ggcaaaacgc tcatcctatc
    10621 cagcaggttc gacaacaaaa cactgatgct ggcagacaat atcaaccaag gcgcaggcgc
    10681 attgcagttc gacagcaact tcaccgtcgt cggtaaaaac cacacatggc aaggtgcagg
    10741 cgttatcgta gccgacggca aacgcgtctt ctggcaagtc agcaacccca aaggcgaccg
    10801 gctctccaaa ctgggcgcag gcacgcttat cgccaacgga caaggcatca accagggcga
    10861 catcagcatc ggggaaggca ctgtcgtact cgcccaaaaa gctgcttcag acggcagcaa
    10921 acaagcattc aaccaagtcg gcatcaccag cggcaggggc acggccgtcc tcgccgacag
    10981 ccagcaaatc aaacccgaaa acctctattt cggcttcagg ggcggacggc tcgacctcaa
    11041 cggcaacaac cttgccttta cccatatccg ccatgcggac ggcggcgcgc aaatcgtcaa
    11101 tcacaaccct gaccaagccg cgacactgac gctgaccggc aaccccgtcc tcagtcccga
    11161 gcatgtcgag tgggtgcaat ggggcaaccg tccgcaaggc aacgcggcgg tttacgaata
    11221 catcaacccg caccgcaacc gtcggaccga ctacttcata ctcaaacccg gcggcaaccc
    11281 gcgcgaattt ttcccgttaa atatgaaaaa ctcaacaagc tggcaattta tcggcaacaa
    11341 caggcaacag gccgccgaac aagtcgccca agccgaaaat gcccgccccg acctgattac
    11401 cttcggcgga tacttgggtg aaaacgcgca aacgggcaaa gccgcgccga gttacagcaa
    11461 aaccaatgaa gcagccatag aaaaaacccg ccatatcgca aatgccgccg tatacggccg
    11521 gcccgaatac cgttacaacg gcgcactcaa cctgcactat cgtcccaaac gcaccgacag
    11581 cacgctgttg ctcaacggcg gcatgaacct taacggggaa gtcttgattg agggcggcaa
    11641 tatgattgtg tcaggcaggc ccgtacccca tgcctacgac caccaggcca aacgcgaacc
    11701 cgttcttgaa aacgaatgga ccgacggcag cttcaaggct gcacggttca ccctgcgaaa
    11761 ccatgcccga ctgacggcag ggcgcaatac cgcgcatctg gacggcgaca taaccgcata
    11821 cgatctgtcc ggcatcgacc tcggctttac ccaaggcaaa acaccggaat gctaccgctc
    11881 ctaccatagc ggcagcaccc actgcacacc caacgccgtt ttaaaagccg aaaactatcg
    11941 tgcactacct gcaacgcaag tacgcggcga cattaccctt aacgaccgtt cagagctccg
    12001 cctgggcaaa gcacacctgt acggcagcat ccgtgccggc aaagacaccg cagtccgcat
    12061 ggaagcagac agcaactgga cactttccca gtccagccac accggcgcac tgacgcttga
    12121 cggcgcacaa attaccctga accccgattt cgccaataat acacacaaca accgcttcaa
    12181 cacactgacc gtcaacggca cacttgacgg gttcggcaca ttccgattcc tgaccggcat
    12241 cgtccgaaaa caaaatgccc ccccctcaaa ctggaagggg acagccgcgg cgcattccaa
    12301 atccacgtca aaaacaccgg acaagaacct caaacaaccg aatcgcttgc acttgtgagc
    12361 ctcaatccga aacacagcca ccaagcccga ttcaccctcc aaaacggcta tgccgatttg
    12421 ggtgcctacc gctacatcct ccgcaaaaac aacaacggat acagcctgta caacccgctc
    12481 aaagaggccg aacttcaaat tgaagccacg cgtgcggaac atgagcgcaa ccaacaggca
    12541 tacaaccaat tacaggcaac cgacatcagc agacaggttc aacatgactc tgacgcgacc
    12601 aggcaggcac tacaggcctg gcagaacagt caaaccgaac ttgcccgcat cgacagccaa
    12661 gtccaatatc tgtccgccca attgaaacag acagacccgc tgaccggcat tctgacgcgt
    12721 gcccaaaacc tgtgtgccgc acaaggatac agtgccgata tctgccgtca ggttgccaaa
    12781 gccgccgaca cgaacgacct gacactcttc gaaaccgaac tggatacgta tatagaacgt
    12841 gtagaaatgg ccgaatccga acttgacaaa gcacggcaag gcggcgatgc gcaagccgtc
    12901 gaaacagccc ggcacgccta cctgaacgca ctcaaccgtc tgtcccgaca aatccacagt
    12961 ttgaaaaccg gcgttgccgg catccgtatg ccgaacctgg ccgaactgat cagccggtcg
    13021 gccaacaccg ccgtttccga acaggccgcc tacaataccg gccggcaaca ggcgggacgc
    13081 cgcatcgacc gccaccttac cgatccgcag cagcaaaaca tctggctgga aaccggtacg
    13141 caacaaaccg actaccatag cggcacacac cgtccctacc aacaaactac caactatgca
    13201 catatcggca tccaaaccgg catcaccgac cgtctcagtg tcggtacgat tttaaccgat
    13261 gagcgcacaa acaaccgttt tgatgaaggc gtatccgccc gaaaccgcag caacggcgca
    13321 catctgttcg tcaaagggga aaacggcgca ctctttgccg cggcagattt aggctacagc
    13381 aacagccgta cccgatttac cgattatgac ggggctgccg tccgccgcca cgcatgggat
    13441 gcaggcatca acaccggcat caaaatcgat accggcatca acctcagacc ctatgccggc
    13501 atccgtataa accgcagcaa cggcaaccgg tacgtactcg acggcgcaga gataaacagc
    13561 ccggcgcaaa tccaaaccac atggcatgcc ggcatccgtc tcgataaaac cgtcgaactg
    13621 ggtcaagcca agctgacccc cgctttcagc agcgattact accatacccg ccaaaacagc
    13681 ggttccgccc tcagcgtcaa cgaccgtacc ttactgcagc aagccgccca cggcacactg
    13741 cataccctgc aaatcgacgc cggatacaaa ggctggaacg ccaaacttca tgccgcttac
    13801 ggcaaagaca gcaacaccgc ccgccacaaa caggcaggaa tcaaaatagg ctacaactgg
    13861 taacaagccg ataaaaatgc cgtctggaac ccgcgtttca gacggcattt gcgttaaaaa
    13921 tagtaaaccg ttccaaaagg gagtagaata gtgccgtttc caaccctgcg cctgtaccgt
    13981 caggctttta ttatggacct tcccagttcg tttttactga acaccccatc cgattcaaac
    14041 ccgcaataac catcccgccg gaatgcctcc ccgcacacgg cgggcggagc atttatgagc
    14101 atcgaaccaa cccctccgaa ccttgaaaac gacggtatcg aaaacgatgt agaacgcgtt
    14161 tccgccgatt tcgaccgtgt ccactccctc tgcgaaatcc tcgaacctgc ttttgaacaa
    14221 atcgaaaacg gtacaccgct cgaagacgcg ccgctgcgcg acaagctgac cgagctgacc
    14281 gtcctcttga gcgagctgca ccctgccgac gtggcggcgg tattggaatc gctaccgccg
    14341 cgcgaacgca atatcgtctg gattctggtc aaaccggaag acgacggcga agtattgctg
    14401 gaagtatccg acgcggtgcg cgaaacgctg atcgagtcga tggacaaaga cgaattgttg
    14461 gcagcggtcg atgatttgga cgcggacgaa ttggcggaac tggcagacga tttgccgcac
    14521 caagtggttt acgaagcgct acaaacccgc gatgaggaag aacgcgccca agtcaaagcg
    14581 gcaatgtcct acgaagacaa ccaagtcggt gcgattatgg acttcgagtt ggtcagcatc
    14641 cgcgccgatg tcgcctgtga agtggtgctg cgctatctgc gccgcttcga cagcctgccc
    14701 gaccataccg acaagatttt tgtggtcgat gaaaacgacg tactgcaagg cgtgctgccc
    14761 atccgcaaac ttttggtcgc cgatcccgaa gatttggtgg aaaacgtgat ggcgaaagat
    14821 gtcgtgcgtt tccgcgccga agacgacgtg gaagaagcgg cgcaggcgtt tgaacgctac
    14881 gacttggtaa ctgcgccggt tgtcgatgaa aacaaaaagc tcatcggtag gattaccgtt
    14941 gacgagatgg tggacgtgat ccgcgaagaa tccgaagcgg atatgttgaa tatggcgggt
    15001 ttgcaagaag aggaagactt attcgccccc gtcttggatt ccgtcaaaaa ccgctggatg
    15061 tggctcgccg tcaacctctg caccgccttc ctcgccagcc gcgtcatcgg cgcgtttgaa
    15121 ggcagcatcg aaaaaatcgt cgcactcgcc gcgctgatgc ccatcgtcgc cggcatcggc
    15181 ggtaactcag gcaaccagac gattaccatg attgtccgcg ccatggcgat ggggcaactg
    15241 acaggcatgc aggcaggcag gctgttgaaa aaagaagtcg gcgttgccct agtcaacggc
    15301 attatctggg gaaccgtcat gggcgcggtt tcatggctgc tctacggcag cctcggcatc
    15361 gggctggtta tgattgccgc gatgacgctc aacctcctgc tggcggcaac cgtcggcgta
    15421 ttaattcccg tggtaatgga aaagttcgga cgcgatcccg cactgggcag ctcggtgctg
    15481 attaccgccg ttaccgactc cggcggcttc ctgattttct tggggctcgc caccctattc
    15541 ctgctttaaa tgccgtctga acccgcgcaa aaatgccgtc tgaagcggaa gctgcttcag
    15601 acggcatttg actatttatc cttgttgcac aagattattg gacggtatgc cggggcagcc
    15661 ctttggcaac gccgaccaca tcctccccga acagcgcgtt gacatcggtt tcgtcaaaca
    15721 catatttgct gtggcagaaa tcgcaatcga cttcgatgct gccttgttcc gccaccacgc
    15781 cgccgacttc ttccccgccc agcatcaaca gcatatcgct gactttgccg cgcgaacagg
    15841 tgcatgaaaa ttcaaacgtt tccggctcga acacgcgcgg cggcgtttcg tggaacaagc
    15901 ggtacaaaac atgttgcgca tccaattctg ccaactcgcc cgccgtcagc gtgcgcgcca
    15961 gcgtactgac gtgttcccat gcctcttcat ccaacacctc ttcaggcaga cgctgcacca
    16021 gcagaccgcc cgccgcttca tcgcttgcag acaggacgat gtgcgtatca agctgttcgg
    16081 aacgtttcat atagttcatc aacatttgcg cgataccgtc gccttccaaa ggcactacgc
    16141 cctgccaggg ttcgccgtct ttgggctgca gcgtcagcac gaatacgccg ccctcgccca
    16201 aaagatcgac caaactttca tcttcaccga tttctgccgc ctcgtcccac cgcgcggttg
    16261 cacggacggt acggtcggaa gtcgcttctg caaccagcat tttcagccgc ccctgcccct
    16321 gaacctgcac aatcagcgtg ccttcgtttt taaggttgcc cgacagcaga acgccggcag
    16381 ccagtagctc acccaaggcg cggcggatgg cggcgggata gtttttctgt tttacaatgt
    16441 gctgccacac gttttccaga cggacgtgca gcccgcgcac gggcatatcg tcgaagataa
    16501 agcgggtacg cacatcggcg cggttgatgg cggtttgatt catgattttc tctgactgat
    16561 tgttcggatg gcggctatat ggttgcggtc ggcgcgaaaa caagacggac ggcggatgcg
    16621 cttcccaaat tatcaataaa ttatataaaa atcaacatat taactcaatc taacaagccg
    16681 ttttttgcca aacagccgtt tttttatata caatcaacaa gatattttcg actgatacag
    16741 cataacatcg cacggcggca cgatgcctcc tgcgcggaaa caccgatatg gattcttttt
    16801 tcaaaccggc agtttgggcg gttttgtggc tgatgtttgc cgtccgcccc gcccttgccg
    16861 acgagttgac caacctgctc agcagccgcg agcagattct cagacagttt gccgaagacg
    16921 aacagcccgt tttacccatc aaccgagccc ccgcccggcg ggcgggcaat gccgacgaac
    16981 tcatcggcag cgcgatgggg cttaacgaac agcccgtttt acccgtcaac cgagtccccg
    17041 cccggcgggc gggcaatgcc gacgaactca tcggcaacgc gatggggctt aacgaacagc
    17101 ccgttttacc cgtcaaccga gcccccgctc ggcgggcggg caatgccgac gaactcatcg
    17161 gcaacgcgat gggacttttg ggtattgcct accgctacgg cggcacatcg gtttctaccg
    17221 gttttgactg cagcggcttc atgcagcaca tctttaaacg cgccatgggc atcaacctgc
    17281 cgcgcacgtc ggcagaacag gcgcggatgg gtacgccggt tgcccgaagc gaattgcagc
    17341 ccggggatat ggtgtttttc cgcacgctcg gcggcagccg catttcccat gtcggacttt
    17401 atatcggcaa caaccgcttc atccacgcgc cgcgcacggg gaaaaatatc gaaatcacca
    17461 gcctgagcca caaatattgg agcggcaaat acgcgttcgc ccgccgggtc aagaaaaacg
    17521 acccgtcccg ctttctgaac tgatttccca aggaatacgc aatgagtatg cccgaaatgc
    17581 ccaaatggta cgacgatgac ggacagatcg tgtcctgtac cgaaaaggtc aaagtgatgt
    17641 ccgaaaatat ggccgagctg tatcagacgg cacaagacgc gtttgaagac gcgctgctga
    17701 tgggttgcgg cgaacgtcag ttgcgcgatt acctgctcgc gctgattgaa ggtttggaaa
    17761 atccctaccg caaagtctga acacgccccg gttgctgcgg cacggtttat ccgtgccgtt
    17821 tttgcgtttg tgcgcggctt cggcttttca gacggcatat ttgacgttat gattaaacag
    17881 ttaacaagat ttatcacaac gccgtcaaaa gacagacaca caacatgaac atcatccgcg
    17941 cgctcctcat catcctcggc tgcctcgccg ccggcgaaac cgccgttttc ctagcaggca
    18001 tcaaactgcc cggcagcatc gtcggcatgg gcgtgctgtt tgcgcttttg caggcgggtt
    18061 gggtcaaaac gtcttggctg caacagctta ccgacgcgct gatggcgaat ctgacgctgt
    18121 ttctcgtgcc gccctgcgtg gcggttatca gctatttgga tttgattgcc gacgattggt
    18181 tttcgatact ggtttccgcc tccgccagca ctttgtgcgt actgctggtt acaggcaagg
    18241 ttcaccggtg gatacggggt attatccgat gaacgaaatc ctcaggcagc ccagcgttct
    18301 gcttttcctc acgcttgccg tgtacgcgat tgcgattatc gtgcgcacgc gcacgggtaa
    18361 tatcttctgc aaccccgtac tcgtcagcac tatcgtgctg attgcctacc tgaaaatcct
    18421 cggtatcgat tatgcggtgt accacaacgc cgcgcaattc attgattttt ggctaaaacc
    18481 cgccgtcgtc gtgcttgccg tgccgctcta ccaaaaccgc cgtaaaatct tcaaccagtg
    18541 gctgcccgtc atcgtttcac agcttgcggg cagcgttacg ggcattgtta cggggatgta
    18601 ttttgccaaa tggctgggcg cggaacgcga agtcgtcctc tcgctcgcgt ccaaatctgt
    18661 taccaacccc atcgccattg aaatcacccg ctccatcggc ggcattcccg ccattaccgc
    18721 cgccaccgtc atcattgccg gtctggtcgg acagattgcc ggttacaaaa tgctgaagaa
    18781 cacggtcgtc atgccctcgt ccgtgggtat gtcgctcggc actgcctcgc acgcgatggg
    18841 gattgccgcc tcgctcgaac gcagccgccg catggcggca tacgcggggc tggggctgac
    18901 gttcaacggc gtgctgaccg cgctgattgc gccgctgctc atccccgttt tggggttctg
    18961 aacccgtttc agacgacatt tcagcccatg ctgtctgaac gccgacacac tcgcaaggag
    19021 aaccgttatg gctgtcaacc tgaccgaaaa aaccgccgaa caactgcccg acatcgacgg
    19081 cattgccctc tacaccgccc aagcaggcgt gaagaagccc gggcataccg acctgacact
    19141 gattgccgta gccgccggca gcaccgtcgg tgcagtcttc acgaccaacc gtttctgtgc
    19201 cgcgcccgtc cacatcgcca aatcgcacct tttcgacgaa gacggcgtgc gcgccctcgt
    19261 catcaacacg ggcaacgcca acgcgggtac gggcgcacag ggcagaatcg atgctttggc
    19321 agtgtgtgcc gccgccgccc ggcaaatcgg ctgcaaaccg aaccaggtgc tgcccttctc
    19381 caccggcgtg attctcgaac cgctgcccgc agacaaaatc atcgccgcgc tgcccaaaat
    19441 gcagcctgcc ttctggaacg aagcggcacg cgccatcatg accaccgaca ccgtgcccaa
    19501 agccgcctcg cgcgaaggca aggtcggcga caaacacacc gtccgcgcca cgggcatcgc
    19561 caaaggctcg ggcatgattc atcccaatat ggcgaccatg ctcggtttca tcgccaccga
    19621 tgccaaagtt tcccaacccg tcctccaact gatgacacag gaaatcgccg acgaaacctt
    19681 caacaccatc accgttgacg gcgacaccag caccaacgac agcttcgtca tcatcgccac
    19741 cggcaaaaac agccaaagcg aaatcgacaa catcgccgac ccgcgttacg cccaactcaa
    19801 agaattgttg tgcagcctcg cgctcgaact cgcccaagcc atcgtccgcg acggcgaagg
    19861 tgcgaccaag ttcatcaccg tccgcgtcga aaacgccaaa acccgcgacg aagcccgcca
    19921 agccgcctac gccgtggcac gttcgccgct ggtcaaaacc gcctttttcg cctccgaccc
    19981 caacctcggc aggctgctcg ccgccatcgg ttatgccggc gttgccgacc tcgataccga
    20041 cctcgtggaa atgtatctcg acgatatttt ggttgccgaa cacggtggac gcgccgcaag
    20101 ctacaccgaa gcacaagggc aggcggtgat gtcgaaggcc gaaatcaccg tccgcatcaa
    20161 gctgcatcgc ggacaagccg ccgccaccgt ctatacctgc gacctgtcgc acggatacgt
    20221 ttccatcaac gccgactacc gttcctgacc cgacacggct tcagacggca tacataaaat
    20281 gccgtctgaa ccgccagaca acataccatg acctccacat tcccccgccg cctcgcccgc
    20341 aaaatccgcc aaacccgccg cctgtcgcgc aaaagcatcg cctttctgtt ccttttggca
    20401 ggttcggcac tcgtcgccct gaccgcgctg ttttttgccc atcttgccga ttttgcgctg
    20461 gaactgaacg ccaaactggt tcaacaatac ccgtggttcg cgtgggtcgc gcttcctttg
    20521 ggtttaccgc ttattgcgtg gctcacacgc aaattcgccc ccttcaccgc cggcagcggc
    20581 atcccgcagg tcatcgcctc actgtcgctg ccctacggcg cacagaaaac gcggctgatc
    20641 cgcctcgggc agacgctgct gaagattccg ctaacctttt tgggtatgct gttcggcgcg
    20701 tccatcggac gcgaaggtcc gtccgtgcag gtcggcgcgg cagtgatggg cgcgtggggc
    20761 gcgtggtgca agaaacacgg cttggcattc aaagggatgc aggaaaacga tttgatggcg
    20821 gcgggcgcgg cgggcggttt ggcagccgcg ttcaacgcgc cgctggcggg cgtgattttc
    20881 gccattgagg aactcgggcg cggcatcatg ttgcgctggg agaggcaaat tcttttgggc
    20941 gtgctcgcct ccggtttcat acaggtcgcc attcagggca acaacccgta tttttccggc
    21001 ttcaacggcg gcgtattgga acatatcttt ctgtgggtcg cactgtccgg cctggtttgc
    21061 ggcgcggcgg gcggcctgtt cggacgtttg ctctatcgcg gtgcggcggc gtttgcaccg
    21121 cgcaagatac gcggcttcat ccgcaaccgt ccgctgctgc tggcggcact gatggggctg
    21181 ctgctcgccc tgctcggcac gttctaccaa ggcaaaacct acggcactgg ctaccacgaa
    21241 gccgcccaag ccctgcacgg catctacgaa gcccccttcg gactcgccgc cgccaaatgg
    21301 ctcgccaccg tattcagcta ttgggcaggc attccgggcg gcattttcac tccctcgctg
    21361 accataggcg cggttttggg cgagcatatc gccgccatcg ccgacatatc gcagggtgca
    21421 aacatcatcg tcctcatctg catggcggca tttctggcgg gcgcgacaca atccccgatt
    21481 acttccgccg tcgtcgtcat ggaaatgacg ggcggacaaa gcctgctgtt ttggatgctg
    21541 attgcctgca ttttcgcctc gcaggtttcg cgccagtttt cgccgcgtcc gttctaccac
    21601 gcatcgggaa tgcgcttccg ccagcgcgtg cttcaagaaa ccgccgccca aaccggcaat
    21661 tcgcccgcaa gaccgcaaac agcaaacagc aaacagcaaa acgggaatgc cgtctgaaaa
    21721 ttaaaacgcc cccgatcaaa cgccggcagc cgccttgatt tgaataccgt tccgccgccg
    21781 cttgaaattt cagcaacaat gccgtctgaa cgacagaatg cggttttcag acggcatttc
    21841 cccatcccga tattgcctaa acaaaaccga agcgtttgct ataattctat tttttaccgc
    21901 atacgcacca atcatgtttc ccgatttctc ccaaaccctc tccaaagacc gccacttcct
    21961 gcgttccgcc ttcaaaaatc ccaacaaata cggcggtttg tccaaaatcg aagaaaaata
    22021 ccgaaaatcg cacgaaatct ttttgaagcg tttggcagcc ttgccaaaac ccgaattcga
    22081 caacaccctg cccgttcacg agaagctcga agaaatcaaa aaagccattg ccaagaatca
    22141 ggtaacgatt atttgcggcg aaaccggttc gggcaaaacc acgcagttgc ccaagatttg
    22201 cttggaactc gggcgtgggg cggcaggatt gatcgggcat acccagccgc gccgtttggc
    22261 cgcgcgctcc gtagcagagc ggattgccga agagctgaaa tccgaaatcg gcagcgcggt
    22321 cggctataaa gtacgcttca ccgaccacac ctcgcgcgat gcctgcgtca agctgatgac
    22381 cgacggcatc ctgctggcgg aaacgcagac cgaccgttat ctcgccgcct acgacacgat
    22441 tatcatcgac gaagcgcacg agcgcagcct gaacatcgac ttccttttgg gctatttgaa
    22501 acaactcctg ccgcgccgcc ccgatttgaa agtcatcatc acctcggcaa cgatagacgc
    22561 agaacgcttc tcccgacact tcaacggcgc gcccgtttta gaagtgagcg gacggacgta
    22621 tcccgtcgaa atcctctacc gaccgctgac cggcaaagac gaagacgacg cagaagtgga
    22681 gttgaccgac gcgattgtcg atgcggcgga cgaattagcg cgacacggcg aaggcgatat
    22741 tttggtattc ctgccgggcg agcgcgaaat ccgcgaagcc gccgaagccc tgcgcaaatc
    22801 caccctgcgc cgcaacgacg aaatcctgcc cctgttcgca cgcctgtcgc acgccgaaca
    22861 gcacaaaatc ttccacccct caggcgcgaa acgccgcatc gtattggcaa ccaacgtcgc
    22921 cgaaacctcg cttaccgtgc cgggcatcaa atacgtcatc gacaccggcc tcgcgcgcgt
    22981 caaacgctat tccgcacggg cgaaagtgga gcagcttcat atcgaaaaaa tctcccaagc
    23041 cgccgcccgc caacgatccg gccgctgcgg acgcgtctcc gcaggcgtgt gtatccgact
    23101 gttttcagaa gaagatttta acagccgccc cgaatttacc gaccccgaaa tcgtccgcag
    23161 caacctcgcc gccgtcatcc tgcgcatggc agcattgaaa ctcggcgatg tggcggcatt
    23221 cccgttttta gaaatgcccg attcacggta tatcaatgac ggttttcagg tgttgttgga
    23281 gttgggggcg gtggaggccg tctgaaaaca ggcagacata aaagaaaatc cgcgtagagt
    23341 gatgtaaact tacccttgct ttaataagta gaaaatggtg ggtttacgtc cccccctgcg
    23401 gctactaaaa aaatataaga gtaaacaacc tttttgaaag aaaaatgtat ggacgaaatt
    23461 caaataccca aaaaagtgga attacaaacc aaactagaaa atgaaaagat tgttttatcg
    23521 aaaggttcta ccacgattat tgttggtgct aatggcacag ggaaaacaag attagctgtt
    23581 tatattgaag aacaattaaa ggaaaaagca cacagaattt cggctcatag agcattaaaa
    23641 ttaaacccta atgtcaataa aataccagaa aagagtgcca aaacatatct atcttatggt
    23701 cagaactggg atggaatcga tgtatcaaat agaaaaaatt atagatggga taataactca
    23761 tatactcatt tactcaacga ttttgattgg ttattacaat atttattcgc tcaacaaaat
    23821 aatattgcgg tagcaaataa tcaaaagctc aaccgtaatg aaaaagtaac caattcaaaa
    23881 acaaagctag atattttgca agaagcatgg gaaacattat taccacacag aaaattacat
    23941 attacagcag atgatattca agtctctgct gtagataatg aggaattgta ttctgcctca
    24001 aatatgagtg atggagagcg agcacttttc tatattcttg gacaagtttt gtcagtagat
    24061 gacggttctg tcttaatttt tgatgagcct gaattacata ttcataaatc aattatttca
    24121 aatctatggg ataaaattga agaattacga cctgattgtt catttctaat cattacacac
    24181 gatattgaat ttgctgcaac tcgagtagct aaaaaatatg ttatcagaaa ttattatccg
    24241 acccctgctt gggatatttc tgaagttcct gaaagtaatt ttgatgaaga aacaataacg
    24301 atgattttag gtagccgtaa gccaatatta tttgttgagg gcaacaataa tagtttagat
    24361 attgctactt accgctattg ttatcctgat tggaccatca tacccaaagg ggcatgcaaa
    24421 gatgtcattc aatcagtatc atcgctgaaa aaattaagta atgaaatgcc attactaaac
    24481 ttaaaatgtt caggtattgt cgatttagat agtagggatg aaagagaaat tgaacaatta
    24541 aataatttgg gtatttacat tttacctgta tccgaaattg aaaatctttt tagcttaact
    24601 gatgtagcaa aagagatatt gaaactaaat caatattcag atgaagaatt actcaataaa
    24661 cttaatggat ttaaatccga actaattaaa tatatagata atgaattaaa agacgataaa
    24721 ttagacgaat ttgttgtaaa acaggttcga cgtaaaattg ataattattt aaaaaatatt
    24781 gatttatcct ccaaaataac aagtactgat atgaaaaaat cattacttaa tgaaatttct
    24841 actttaacag aacagaaaat tgaaacatgg atttcagaaa ttaaaaatga aattcaaaga
    24901 tgtattgaac agcaagattt ggataaatta cttactatat atgataataa aggactcttg
    24961 gctaaatcag cttgtgtttt aaaaggaatg cgtaacaaac atgaatttga aagctggata
    25021 atgagaacat taaaaggaag gaataaagat tttattgatg caatcagaca gaaacttcca
    25081 attctggatt aaataaaacc atctgaaaat ttaccttcag atacagatat atttcatgaa
    25141 aaatcatcaa actacactct ctttccctac ttcgagtagc ctgaaacctt gcgcagacaa
    25201 acaaggcctg tctgaagacc gcagccaata ccgcctgacc aaactcggcg aacaaatggc
    25261 gcacctgcct atcgacccga aaattgcgcg tattttgtta gtattattcc gtttttaaaa
    25321 atgcccgatt cgcggtatat caatgacggt tttcaggtat tgctggaatt gggggcggtg
    25381 gaggccgtct gaaaataaaa tctttcttta taaaaaggca ggccatgttt cattttcaga
    25441 cggcctaaat cattgagaaa ctaaaaacta ttaaaaaggg aatattgggt tttaaaactc
    25501 aatcggtaaa tttttattgt gaaatattaa tgatgaaaaa atctttcctt acgcttgttc
    25561 tgtattcgtc tttacttacc gccagcgaaa ttgcctatcg ctttgtattt gggattgaaa
    25621 ccttaccggc ggcaaaaatt gcggaaacgt ttgcgctgac atttgtgatt gctgcgctgt
    25681 atctgtttgc gcgttataag gtgacgcgtt tgttgattgc ggtgtttttt gcgttcagca
    25741 ttattgccaa caatgtgcat tacgcggttt atcaaagctg gatgacgggc atcaattatt
    25801 ggctgatgct gaaagaggtt accgaagtcg gcagcgcggg tgcgtcgatg ttggataagt
    25861 tgtggctgcc tgtgttgtgg ggcgtgttgg aagtcatgtt gttttgcagc cttgccaagt
    25921 tccgccgtaa gacgcatttt tctgccgata tactgtttgc cttcctaatg ctgatgattt
    25981 tcgtgcgttc gttcgacacg aaacaagagc acggtatttc gcccaaaccg acatacagcc
    26041 gcatcaaagc caattatttc agcttcggtt attttgtcgg acgcgtgttg ccgtatcagt
    26101 tgtttgattt aagcaggatt cccgccttta agcagcctgc tccaagcaaa atcgggcagg
    26161 gcagtgttca aaatatcgtc ctgattatgg gcgaaagcga aagcgcggcg catttgaagc
    26221 tgtttggcta cggacgcgaa acttcgccgt ttttaacccg gctgtcgcaa gccgatttta
    26281 agccgattgt gaaacaaagt tattccgcag gctttatgac tgcagtgtcc ctgcccagtt
    26341 ttttcaatgc gataccgcac gccaacggct tggaacaaat cagcggcggc gataccaata
    26401 tgttccgcct cgccaaagag cagggctatg aaacgtattt ttacagcgcg caggcggaaa
    26461 acgagatggc gattttgaac ttaatcggta agaaatggat agaccatctg attcagccga
    26521 cgcaacttgg ctacggcaac ggcgacaata tgcccgatga gaagctgctg ccgttgttcg
    26581 acaaaatcaa tttgcagcag ggcaagcatt ttatcgtgtt gcaccaacgc ggttcgcacg
    26641 ccccatacgg cgcattgttg cagcctcaag ataaagtatt cggcgaagcc gatattgtgg
    26701 ataagtacga caacaccatc cacaaaaccg accaaatgat tcaaaccgta ttcgagcagc
    26761 tgcaaaagca gcctgacggc aactggctgt ttgcctatac ctccgatcat ggccagtatg
    26821 ttcgccaaga tatctacaat caaggcacgg tgcagcccga cagctatctc gtgccgctag
    26881 tgttgtacag cccggataag gccgtgcaac aggctgccaa ccaggctttt gcgccttgcg
    26941 agattgcctt ccatcagcag ctttcaacgt tcctgattca cacgttgggc tacgatatgc
    27001 cggtttcagg ttgtcgcgaa ggctcggtaa cgggcaacct gattacgggt gatgcaggca
    27061 gcttgaacat tcgcgacggc aaggcggaat atgtttatcc gcaatgagtg gcgtaaaaac
    27121 caataaagac aaatttagat gatgtcgggg aagatgcccg accgacaaga ctatgcaaaa
    27181 tatgaaaaac caagtacgcg gatcaggcat ggatgcccga tccaatccgg ccaatgtttc
    27241 agacggcctg caaaacagtt cgggtcatat cggtaccaac acgcgttacc gcctgaccaa
    27301 actcggcgaa cagatagcgc gcctacccat cgacccgaaa atcgcgcgca ttttgctggc
    27361 ggcgaagaaa cacgactgca tggcggaaat attggtgatt gcgtccgcgc tgtcgattca
    27421 agacccgcgc gagcggccgc tagaagcgcg cgatgcctca gccaaggcgc acgagcgttt
    27481 taccgacaag cagtccgatt tccttgccta tctgaacatt tgggacagct tccagcgcga
    27541 acgcgataaa ggcttgtcca acaagcagct ggtgcagtgg tgccgccaat atttcctgtc
    27601 gcacctgcgg atgcgcgagt ggcgcgagct gcaccaccag cttgcccaaa ccgcgattga
    27661 aatgggttta accaccaagg aagccgcttt cagacgacct cccgaagtca ggcagctcac
    27721 gtcgtctgaa aatgcgggtg accaagacct atctgctaaa ctcaaacaaa aacaactgga
    27781 taaaaagcaa caccgcgccc aaatccgcgc cgccaaagaa gcgggctacg aacaaatcca
    27841 ccgcgccctg ctcactggcc ttatcgccaa cgtcggcatg aaatcgcccg acggtaacga
    27901 ctacaccggc gcgcgcggca gccgcttcca ccttttcccc gcctccgccc tgttcaaagc
    27961 caaacccaaa tgggtgatgg cggcagaatt ggttgaaacc acgcgccttt acgcgcgcga
    28021 cgtcgccgtt atccagcccg aatggataga gcaggaagcg ccgcacctcg tccgctatca
    28081 ttatttcgag ccgcattggg aacaaaaacg cggcgaagtc gtcgccagcg aacgcgtgac
    28141 gctttacggt ctgaccgtat tgccgcgccg ccccgtgtct tacggcaaag ttgcccccga
    28201 agaagcgcgc gaaatcttta tccgcagcgc gttggtggcg caggaatgcg atttgaaagc
    28261 ggattttttt gtccacaaca aaaagctgat taaagaaatt accgaactcg aacacaaatc
    28321 gcgcaagcaa gacgtgctgg tcgatgacga agccctgttt gcgttttata acgaacgact
    28381 gcccgaaatg gcttggaaag acgcgcaagg cagcgtttgg ggaagtgaag attccgtacg
    28441 gattattgaa tctgacaaag ccgagaggtc gtctgaaaat gagcgcaacg agtttcgtaa
    28501 aaacaagcgt aatgggtctc gccaaaatga aaatcacggc aacaccgtag gttgggttga
    28561 aaacccaaca tcagccgcaa ctgcaaaaac tgttgggttt gacaatccaa cctacgctgc
    28621 ccaacaaacc accccctccc ccgtggggga gggtcgggga gagggcaaaa cagttgccgc
    28681 acaaaccaac ttttccgcaa ccgcagcaaa ccctctccct aaccctctcc cgcaggagag
    28741 ggaacagagt gccgcagctt caacgatttc agacgacctg cgtcctgcaa atctgcagca
    28801 aaccgccccc tcccccgtgg gggagggctg gggagagggc aaaacagttg ccacacaaac
    28861 caacttttcc gcaacctcaa caaaccctct cccgcaggag agggaacaga gtgcctcagc
    28921 ttcaacgttt tcagacgacc tgcgtcctgc aaatctgcag caaccctctc cctcccccgt
    28981 gggggagggc tggggagagg gcaaaacagt tgccacccaa accaactttt ccgcaacctc
    29041 aacactttca gacgactcca aacccaaaaa gcagcctgca ccccaaaaaa accgtctgaa
    29101 acccctaccc ctcgccgaca tccgcacctt ccaagcctgg ctcaaaaccg ccgaacgcga
    29161 caatctgcgc ctgctgttcc tcagccgcga cgatttgatg caacacgccg ccgcacacat
    29221 taccgaagaa cagttcccca aattctggca aaccgcagac ggcaagttca aactttccta
    29281 ccgcttcgag ccgcaccatc cgctagacgg cgtgaccatg accgtgccgc tgaccgtcct
    29341 caaccgcctg cacgcgccgt cgctcgaatg gctggtgccc ggcatgatac gcgaaaaaat
    29401 ccagttgcaa atcaaagcac tgcccaagca aatccgccgc atctgcgtgc ccgtacccga
    29461 attcatcacc caatttttaa gccaaaaccc cgaccgcaac gcccccatcc tgccccaact
    29521 cgcccaagcc atcgccaaaa ccgcaggcga catccgcata ttcgagcaaa tcaaccaaga
    29581 cgaatgggcc gcgttcaggc tgcccgaaca ctgctatttc aacctccgca ttatcgacga
    29641 cggcggacaa gagcttgccg gcggccgcaa actgcacgaa ttgcaacaac aactcggtca
    29701 agctgccgcc gttaccttcc gtgacaacac ccaagaattt gagcgcgaca acgtcaccgc
    29761 atgggacatc ggcaccctgc ccgaatccat caaattcgca cgcggcaaac aacagctcac
    29821 cggctatctc ggcctacaaa aagaaaaaga cggccgcatc gccctgcgcc tgtttgatac
    29881 cacagaagcc gcagagcagg cacaccgtca aggtgtcatc gaattgatga agctgcaatt
    29941 aaaagagcag gtaaaggatt tgaacaaagg catccaaggc ttcacccaag ctgccatgct
    30001 gctcaaacac atcaacgccg acactctgcg cgacgacctc acccaagccg tctgcgaccg
    30061 cgcctttatc ggcgaagacg agctgccgcg caacgaaaaa gccttcaaag aacaaatcaa
    30121 acgcgcccgc agccgcctgc ccgccgtcaa agaagccctc agccgctacc tgcaggaaac
    30181 cgccgccgtc tacgccgaac tcaacagcaa actcggcaaa cacccattga cccaccttct
    30241 aagactacgc ctgcaaaccc tgctcgccgc cggcttcgcc acccgaaccc cgtgggcaca
    30301 atggccgcgc ctccccatct acctcaaagc catgaccctg cgcctcgaaa aatacagcag
    30361 caaccccgcc cgcgacgcag cccgcgaagc cgatatccaa gagctggaac aaatgtggca
    30421 ggaaaaaaca gacagcctga ttaaacaagg tctccccatt tcagacggcc tcgccgcgtt
    30481 taaatggatg attgaagaat tgagggtgtc gctgttcgcg caggaattga agacaccgta
    30541 tccggtgtcg gtgaagcggc tgttgaaaga gtgggaaaaa attgaaaaat aaaaaaacag
    30601 cctgaaaagt ttcaggctgt ttttttattt gactaatcga agtttcctat atctatttaa
    30661 gtccctctca actaatccaa aagttaaatc agcaacatct ttgggggata cgtttaaatt
    30721 ttcagcaatc tgttcaatac caatgccatc attttttaaa atagtaagca ttttacgtaa
    30781 tgcgcttgat atttcccttt ccattggctc tggttcgata gttcgatatt ttttctttgc
    30841 aaacaaagga caaagattgt gtatatacat cctatcagta atcattccta atttatgcat
    30901 ccgatatgct aaggcaacaa gtgatacacc aaatcgtctt ttgattttta ataaattttc
    30961 aatagtgata ggaacatgac gatataagcg tagtgcagcc tccggcatta aaaaagctga
    31021 agcaaaggca ttagcctctt tttcgataat atcacgaggt tcatcttctg taatttcact
    31081 atttttacta tgttccatac tgtatttatc acggattaag tgccctaatt catgggcagc
    31141 atcaaatcga ctacgttctg cagatttttg tgtatttaaa aatacaaatg gatgattttc
    31201 ataccaagta caaaaggcat caatgtcctt tgtatctaaa gataatgaaa atacacgaac
    31261 acccttagct tcaagtaggg tgatcatatt cggaataggt tcattgccaa gcccccattc
    31321 taatcttagt tcctgagcag cctcttcagg agaaatatca gaaaaatcag gcaatacggc
    31381 ttgacttagt gtaaattctg tctcgagcca gtcatttaac aaaaaagccg taatgctatg
    31441 atttaatgct tgtttttcaa gcctcttcga ggtgcgtgaa cgagcacgaa aacttactgc
    31501 ctgagatttc aactcaggca gtctttcgtc attagtaaag aaatgaactg gaaactctaa
    31561 taaattggct aattcattta aatcaggtat ttgctcatct tttacatagt ttctaacctg
    31621 tcgagcggta atacctaata actcagctaa ttttgtttgc gtacaaccac gtttatccag
    31681 cgcaaattcc agtctctcac gattaaatgt ctgcatgatt tatcattcaa attatcctgc
    31741 tttttgtttt ctttcaacca aaggctcata ttcttcaaca ggttgcttac gttcaagctc
    31801 atcaaattta gttaaatcaa catcagctaa tataattcgc tgcttgtacc cagttatttg
    31861 atgactaaca aaaccactcg gtaaagataa ttcaagttgc actttattat acttccagtg
    31921 aaacagcaga acccaaaact gcacagtatc aggcaaatct agttttgaat tacgaatagc
    31981 ctcctcaaat cccttacctt tccttgcggt tgtcattggc atcccgtgat gcctaccaac
    32041 atctgaagta gcagtagcca caataatact tttagttcga catggcgaga gacatagaaa
    32101 tgcaccaccc gaccaaggct caagcgtcca gccatcttta cttaaatata cccttagagc
    32161 aaatgtaatt tctgcttgcc gatacatccc caatgtattt ctgtcagata atgctgcttt
    32221 gtcctgaata ttattatgcg cagtaagtac aatctcttta agcatctcct gagataagta
    32281 cttgctgatt tcacttaaag caatatcact attttgttgc tcgactattt ctcctacttc
    32341 aaatgggaaa ggttctgata atgcaaattc caccataaaa atttcctaat tttatacgta
    32401 atgtttacac aatatatcag gaaatatgaa aacgtacaac tatatctata aagcaattaa
    32461 taagtagcct gcccaaccgt gtccttatct ttcggcacac ccgacctgca aatcacgcaa
    32521 aacttggaat ccgtgtgtag ggtgtgtgcg gtacatacgc acgcagtctt tttaaaccac
    32581 agcccttccc aactaaacca aaaggtcgtc tgaaccctat tttcagacga ccttttgcca
    32641 ctttgtaaaa caaatcttcc caccatcctc tccccaaaca tcgcccgaac cagtaaactt
    32701 ctcatatttc aacaactcct tggaagcaaa ccatgtctgg tatctaccta ccccgcctat
    32761 tcccgcccca tatcgccgaa cgcggcctgt tgtattttca gcagggcaag gttctcgatg
    32821 tccgaaaaac ttccgccggg cattatcggg cggaggtgtg cggttcggaa aactattggg
    32881 tatagttgaa gctggatagt gatttgtata ttaaagacga aggctgcaat tgtccttata
    32941 tctaagagtg caaacatacc ttaaattact atattgcata ggcaaaatac aagcctataa
    33001 cgaattggaa acaaaatgcc gtctgaaaac atcttcagac ggcattataa aatctgttca
    33061 ccttttcaga tgagtaatgt acacccttat acaatttttg ctactatgcc ccataaatcc
    33121 acggctaaag atatccttat tatgtcctat gatttatcga aacgacttgt aatcggctta
    33181 gcatcaagtg ccctattcga cttatccgaa tcggataata tatttagaat ggaaggggca
    33241 gaaacctata ggcaatatca gagagaaaaa caaaaccatc ccctaaaaaa ggcgttgtct
    33301 ttccatttat taaaaaactt ctgtcaatca atgaaataaa cccaaacgac ccaacgattg
    33361 ggtttattct tttatccaga aacaatccag atacagatta cgagtcataa ctataggctt
    33421 aatattacac gattctcatt ccatcaaggc ggaaaaccgc acaaatactg aaacactatc
    33481 gatcgatttg taaacaagcc tacttaagta acttgcagtc cttatcattt cctttaaaat
    33541 aatccagccc gtcactacac gaactggcgg acttcttgca aataaaggtt actagatttt
    33601 cattcatctt aataataaaa ggatttttat ctttatctat ggctaccgcc ttcaacatga
    33661 atttactgtc taaagccccg cgcgcgattc cattcaaacg gatacaaaag ccttctgcct
    33721 ctttaatcgg caaacttggc cacttggtag atgtttgttt aaacctccca ttctgcagat
    33781 aaaacttttc cataaaatgt gcattttcta acaaggctgc ccgcactgca tttatctttg
    33841 ctttctcaac ataattgcga tagctcggat aaacaattaa agcaagtaca gacaatatca
    33901 agaccactga tattaattca accagcgtaa acccccgatt atcagtcatt actttacttc
    33961 caataagaac agattattca acatatttct ttgaacagac ttactatccc attcaacagt
    34021 atgcatattt cccactctat tttttagcgg ccggtatagc cggtttagct gggccttttg
    34081 gtgcgggcgc gccggcagaa gcctgatcct tcagcttcgc cagcaccgca gggccgatgc
    34141 cctttacctt ggtcaaatcg tc
//
LOCUS       DAWHRS010000005        32203 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWHRS010000005 DAWHRS010000000
VERSION     DAWHRS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32203
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.005"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..105)
                     /locus_tag="WIB82_000162"
     CDS             complement(<1..105)
                     /locus_tag="WIB82_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ3841538.1"
                     /translation="MIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTRYL"
     gene            complement(273..2201)
                     /gene="rpoD"
                     /locus_tag="WIB82_000163"
     CDS             complement(273..2201)
                     /gene="rpoD"
                     /locus_tag="WIB82_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ3841539.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(2396..4168)
                     /locus_tag="WIB82_000164"
     CDS             complement(2396..4168)
                     /locus_tag="WIB82_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ3841540.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQLYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITASALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(4146..4298)
                     /locus_tag="WIB82_000165"
     CDS             complement(4146..4298)
                     /locus_tag="WIB82_000165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841541.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            complement(4313..7063)
                     /gene="secA"
                     /locus_tag="WIB82_000166"
     CDS             complement(4313..7063)
                     /gene="secA"
                     /locus_tag="WIB82_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ3841542.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLMDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            7194..7616
                     /locus_tag="WIB82_000167"
     CDS             7194..7616
                     /locus_tag="WIB82_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ3841543.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLILLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESLDSAAAKLEKRHPELAEALANLVRRHGA"
     gene            7897..8448
                     /gene="azu"
                     /locus_tag="WIB82_000168"
     CDS             7897..8448
                     /gene="azu"
                     /locus_tag="WIB82_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ3841544.1"
                     /translation="MKTYLALISAAVIGLAACSQEPAAPAAEATHAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            8543..9052
                     /locus_tag="WIB82_000169"
     CDS             8543..9052
                     /locus_tag="WIB82_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ3841545.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            9086..9706
                     /locus_tag="WIB82_000170"
     CDS             9086..9706
                     /locus_tag="WIB82_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ3841546.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            9820..10965
                     /gene="dapE"
                     /locus_tag="WIB82_000171"
     CDS             9820..10965
                     /gene="dapE"
                     /locus_tag="WIB82_000171"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ3841547.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLVERLYKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            11337..11616
                     /locus_tag="WIB82_000172"
                     /pseudo
     CDS             11337..11616
                     /locus_tag="WIB82_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(11705..12228)
                     /locus_tag="WIB82_000173"
                     /pseudo
     CDS             complement(11705..12228)
                     /locus_tag="WIB82_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            12578..13330
                     /locus_tag="WIB82_000174"
     CDS             12578..13330
                     /locus_tag="WIB82_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ3841548.1"
                     /translation="MITLPSLNNLPSKWDEIRHWLESRVFECGVMPHPNLTECEAKQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGSRGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALEHGEVQTALF"
     gene            13522..13597
                     /locus_tag="WIB82_000175"
     tRNA            13522..13597
                     /locus_tag="WIB82_000175"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13555..13557,aa:Asn,seq:gtt)
     gene            13728..14738
                     /gene="waaF"
                     /locus_tag="WIB82_000176"
     CDS             13728..14738
                     /gene="waaF"
                     /locus_tag="WIB82_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ3841549.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENSFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            14793..15239
                     /gene="smpB"
                     /locus_tag="WIB82_000177"
     CDS             14793..15239
                     /gene="smpB"
                     /locus_tag="WIB82_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ3841550.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            15658..17636
                     /locus_tag="WIB82_000178"
                     /pseudo
     CDS             15658..17636
                     /locus_tag="WIB82_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(17817..19184)
                     /locus_tag="WIB82_000179"
     CDS             complement(17817..19184)
                     /locus_tag="WIB82_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ3841551.1"
                     /translation="MLDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPLS
                     VKSVQTIMRFCHQHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFANPLDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINSTVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(19294..19560)
                     /locus_tag="WIB82_000180"
     CDS             complement(19294..19560)
                     /locus_tag="WIB82_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841552.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPAAEAPAA
                     EAPAAEAAATEAPAAEAAATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            20033..20515
                     /locus_tag="WIB82_000181"
     CDS             20033..20515
                     /locus_tag="WIB82_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3841553.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            20602..21414
                     /gene="hpnC"
                     /locus_tag="WIB82_000182"
     CDS             20602..21414
                     /gene="hpnC"
                     /locus_tag="WIB82_000182"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ3841554.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LNGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(21487..21957)
                     /locus_tag="WIB82_000183"
     CDS             complement(21487..21957)
                     /locus_tag="WIB82_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ3841555.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            22048..23889
                     /locus_tag="WIB82_000184"
     CDS             22048..23889
                     /locus_tag="WIB82_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ3841556.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            complement(24300..25496)
                     /locus_tag="WIB82_000185"
     CDS             complement(24300..25496)
                     /locus_tag="WIB82_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ3841557.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            25847..26146
                     /locus_tag="WIB82_000186"
     CDS             25847..26146
                     /locus_tag="WIB82_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841558.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEDGAGN
                     HVLSPFAQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNI"
     gene            26146..26331
                     /gene="ccoS"
                     /locus_tag="WIB82_000187"
     CDS             26146..26331
                     /gene="ccoS"
                     /locus_tag="WIB82_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ3841559.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDVR"
     gene            26321..27631
                     /locus_tag="WIB82_000188"
     CDS             26321..27631
                     /locus_tag="WIB82_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3841560.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAIYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKEP"
     gene            27789..28523
                     /gene="dnaQ"
                     /locus_tag="WIB82_000189"
     CDS             27789..28523
                     /gene="dnaQ"
                     /locus_tag="WIB82_000189"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ3841561.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGEAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            28520..29209
                     /locus_tag="WIB82_000190"
     CDS             28520..29209
                     /locus_tag="WIB82_000190"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ3841562.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            29241..29723
                     /locus_tag="WIB82_000191"
     CDS             29241..29723
                     /locus_tag="WIB82_000191"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ3841563.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            29800..30471
                     /gene="rpiA"
                     /locus_tag="WIB82_000192"
     CDS             29800..30471
                     /gene="rpiA"
                     /locus_tag="WIB82_000192"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ3841564.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            30540..31211
                     /locus_tag="WIB82_000193"
     CDS             30540..31211
                     /locus_tag="WIB82_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ3841565.1"
                     /translation="MQIKKIMKWFPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            31390..32136
                     /locus_tag="WIB82_000194"
     CDS             31390..32136
                     /locus_tag="WIB82_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ3841566.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
BASE COUNT         7323 a         7553 c         9169 g         8158 t
ORIGIN      
        1 aaggtaacgg gttctttgac ggttcaggta ttgttcgatc ggctgccaat caatcacctg
       61 gtccaacttc aatagcggga aacggtcgat gtgtttggca atcatggctt gggcggtttg
      121 ttggaagaag gtgctcctaa atgtcttggt gggaatttag gggattttgg gggattttgc
      181 aaaggtctcg ggataggagt gtgggatatg tggaagcgtt gtttattgtg taagaaaaaa
      241 ccgcaggtat ttgaaacctg cggttttttg gtttacagct tgctgtcttc gctgtccaag
      301 aaacttctca aacggtcgct tcttgtcgga tgccgcagct tgcggagtgc ttttgcctcg
      361 atttgtcgga tgcgttcgcg cgttacgtca aactgtctgc cgacttcttc cagcgtgtgg
      421 tcggtgttca tatcgatgcc gaaacgcata cgcaggactt ttgcctcacg cggtgtcagg
      481 ctttcgagga tttctttggt tacttcgtgc aggctggtgt acattgccgc atcggccggc
      541 gcaacattgt tggcatcttc gatgaagtcg cccaagtgcg aatcgtcgtc gtcgccgatg
      601 ggggtttcca tcgaaatcgg ctctttggcg attttcatga ttttgcggat tttgtcttcg
      661 ggcatctgca tcagttcggc aagtttggcg gaatcgggtt cttcgccggt ttcttgaagg
      721 tgttggcgcg agatgcggtt catcttgttg atggtttcaa tcatatgtac cggaatgcgg
      781 atggtacgcg cctgatcggc aatcgaacgg gtgattgcct ggcggatcca ccaggttgcg
      841 taggtggaga atttgtagcc ccggcggtat tcgaacttgt cgaccgcttt catcaggccg
      901 atgttacctt cctgaatcaa atcaaggaac tgcaagccac ggttggtgta ttttttggca
      961 atggaaatga cgaggcgcaa gtttgcctga atcatttcct gtttggcggc tgcggtttct
     1021 ttttcgctcg acaccatatt tttgttgatt tctttcaact cttcgatgga aatgcgggtt
     1081 tctttttcca tatctgccaa ctcggtttgt ttttcgagga tggcgtggcg gaagcggtcg
     1141 agcgcgtcgc tccaaaccct gcctttggcg atttcttctt caatccattg cagattggtg
     1201 atttcgggca ggaagttttg gatgaagtag tcgcgctcca tatggacgcg gtcgaggcag
     1261 atgtcgcgga tttcgcgttc gagtttgcgg atgttttcta cttttccgcg caggctgctg
     1321 ctgaggctgt cgatttgccg ggtggcgaaa cggacttcca gcagtttgtt ggcaatcgcg
     1381 tcgcggtagg cgagatagtc tttgtgccgg ctgtggtgtt tttccaaacg accgatcatt
     1441 tttttgtagt ctttttcgat ttgggcaaag tggccgatga ctttttgttt caattcggcg
     1501 agattggctg ccgagatttc atccgcatct tcttccgatt cttcgtcgtc ttcgttttcg
     1561 tccgaattgt cgttggaagg tttctcgggt gctgtggttt ccaagtgccc caagcccaat
     1621 tcgttgagca atacttcatt cgggtcgata atggcttcta cgacttcgtc gacgcggatt
     1681 tcgtctttgc ggattttttc gatgagttct aagatttcag caatggatcc cgggcaggcg
     1741 gagatggcct gaaccatatt tttcagggcg ttttcgattt ttttggcgat gatgatttcg
     1801 tcttcgcggg tcagcaggtc gacctgtccc atttcgcgca tatacatacg gacggggtcg
     1861 gtggttctgc cgaactcgga atctgcactg gaaagggcgg cctcggcttc ttcgacggca
     1921 tcatcgtcgg taacggcggc attgtcgctt aacaatatgt cttccgcatc gggggcgtgt
     1981 tcggtaactt ggatgcccaa accggaaatc atgctgacga tattgtctat ttggtcggca
     2041 tcagacatat cgtctggcag ggcgtcgttg atttcggagt aggtgatgta gccgcgttct
     2101 ttacccatga tgatgagctg acgcaggcgc gcgcgttgct cttcaatgct taacggacgg
     2161 gtgtcgtctt gatattcttc gtgattttgg tttctggaca ttgtctgctt tctgttggta
     2221 agatgccgac ggtttgcgtt tggacgcacc tgaaggcgtg gcagagtagg gtactctgat
     2281 gccgtctgaa ggcttgaagg cttttgggga tgggtgttct gcaggtgtcg ttgttgcagc
     2341 cggggtgaat gcttttcaga cggcataacg gtttacggat ggcggaatcc gccggtcaat
     2401 tttgttttgc ggtcagcagc gacagcaaaa gttttttctc gctttcattt aagccggatt
     2461 gcaggctttt ttgttttaat gtttcgattt ggctgtattt taactcattg agcagttttt
     2521 tgatgccgat ttggaagttt tcgcaatctt cttcgccgcc gccttccatt tcttccgatt
     2581 gaagggccga ccggaagatg cggttgattg tttcttcgta gggcgaaccg cgcatatgtt
     2641 ccaaaacctg tgcggttgcc ggcacggaag gatggttttt aatggtttcg gcgaggttgg
     2701 caaggcaggc gaaatcgccg tccaacgcca gataatcggg caggtctata tatgcagccc
     2761 aatccggatt tatcaagagg ctgcggattt gccgttgcac caatgtcggc ataacgggct
     2821 gtttgacgga aatcggcggc agtttgtagt ttttttgttt gacgtgccgc ttcggtgctt
     2881 cctgtcccag cagttgcgcg aggttgtcgg ggtcgatgcc gaccagttcg ctaagccgtt
     2941 gttttaacaa ataagccaat gccgacgcgg taatctgcgc caaaagcggc gaactggttt
     3001 ttaccaattc cgccttgcct tcctgcgtat tgagatgaat gccgtctgaa aggtgttccc
     3061 agaaatattc cgacaagggc ttgctttgat tcaacagcgc gtcttcaaat tgcgctttgc
     3121 cgtaggcgcg gatgtagctg tcggggtcgt gttcttccgg caggaacaaa aaatgcagcg
     3181 atttgtcgtc cttcaattgc ggcagcgcgt tttccagcgc gcgccaagcc gctttgcgcc
     3241 ccgcgctgtc accgtcgaaa cagaaataaa tactgtccgc ctgacgcatt aggattttga
     3301 cgtgttcagc tgttgttgcc gtgcccaagg ccgccacacc gtagcccacg ccgaactgtg
     3361 ccagcgcgac cacgtccata tagccttcga ccaccagaat ccgctccgct tccttgacag
     3421 cggcacgccc ctcatacaag ccgtaaaggt ttttcccttt gtcaaacaaa ggtgtatcgg
     3481 gagagtttag atatttgggt ttggagtcgt ccagcaccct gccgccgaaa ccaattacct
     3541 gcccgcgcgg attgcggatg gggaacataa tccgatgacg gaatcggtcg taataccgcc
     3601 cctcattgtc aatcaccata cccgtatcca ccaacgcggt attcggatat agttggaaca
     3661 cttgcgccaa aggctgccag ccgtcgggcg cgtagcccaa accataatgc gcgatgactt
     3721 ccgcgctcaa gccgcgcttg tccaaatagg cttttgccgc tggattgaat tttagctgtt
     3781 gcgcataaaa atcagctgcc gcagccgtcg tttcctccag cgtctgctgt tttttcttgc
     3841 gctcggcacg gacttcggga ttatcgtttt gcccgcgcac tttaggcaca atcataccca
     3901 cgcggtcggc aaggaactgc accgcctccg gaaacgacag cccctgatgt tccatcacaa
     3961 aaccaatcgc cgaaccgtgc gccccgcaac tgaaacagtg gtaaaactgt ttggtcggac
     4021 tgaccgaaaa cgacggcgtt ttttccttgt ggaacgggca acacgccata tagttcgccc
     4081 cgcctttttt cagcggaacc tgctcgtcga taatatcgac aatatcggtt ttggctaaaa
     4141 gctcgtcaat gaagtcggat ggaatcatga tgtggggcgg acgggatgtc tgtttttggt
     4201 acggacggta cgaaatcggg atttttcgga atggcagtcg gatgtggcgg ttcaggcaaa
     4261 atgccgtctg aaacgggagc gggatgttca gacggcattt gcgttcaaac gcttaagcca
     4321 gtttgccgtg gcattgtttg tatttcaaac cgctgccgca ggggcagggg tcgttgcggt
     4381 ggacgatttg tccccgcgct tccaaggctt cggggctgaa atccgttcca tcgggattaa
     4441 aggcctcggt aaccaaatcg gtttgcgact gacccaaaag ttcttccata tcgggcgatt
     4501 cggaatggat ggactggatg ttgccgatgg gttgctcttc aaccaccgcg acagggtttt
     4561 gttcgatttg aaccgaggta agcagggagg caatatggaa tttgatgccg ttccacaggt
     4621 cttggaacat ggtaaaggct tcacgtttat attcctgctt cggatttttt tgggcatagc
     4681 tgcgcaggtg tatgccttgt cgcaggtagt ccatagcggc gaggtgttcg cgccattggt
     4741 tgtcgatgac ctgcaacatc acgttgcgct cgaaatcggc cattgcctgc ttaccgacca
     4801 gttcggtttt ggcggcatat tcgttttcga tgcgttcgat caggcgttct ttgatgtctt
     4861 gaccgtcaat cgcattgtcc gccttcagcc aggattggat gtcttcgtgc agtctgaatt
     4921 cggcagccag acggttctcc aaagtcggga tgtcccattg ttcttccatg ctgtcgggcg
     4981 gcatataggt atccatgagg tcgctgacga catcagaacg gatttccttc atcaggtcgc
     5041 tgatgtcttt gctggtcaga atttcgttgc gctggctgta aatgacttta cgttgctcgt
     5101 tggcaacgtc gtcgtattcc aaaacctgtt tgcgcatatc gaagtttctg ccttcgactt
     5161 tgcgttgcgc cccttcgatt tggcgcgtca gcaggttgtg ctcgatggca acgccgcgtt
     5221 cgggggcgag gcggttgagg atggcggcgg cgcggtcgag tgcgaatagg cgcagcagcg
     5281 ggtcttcaaa cgagaggtag aaacggctgg atccggggtc gccctgacgg ccggaacgtc
     5341 cgcgcaactg gttgtcgata cggcggcttt cgtggcgttc cgtaccgatg atgtgcaaac
     5401 cgcctgcttc catcactttg tcgtgttctg cctgccagcc gtcttcgagt gcggagattt
     5461 gtgcctgttt ctcttcgtcg ctcaaggctt cgtcggcacg gatggcatcg gtttggtgct
     5521 tcaggttgcc gcctaaaacg atgtccgtac cgcgtcccgc catattggtg gcaacggtaa
     5581 tcgcgccgac tttgcctgcc tgggcgacaa tcagcgcttc gcgttcgtgt tctttggcgt
     5641 tgaggacgtt gtgtggcagt ccggcttttt gcagaaggtg ggagaccagt tcggagtttt
     5701 cgatgctggt cgtaccgacg aggacgggct gcccgcgttt gtggcattct tcgatgtctt
     5761 tgacgacggc ttcgaatttt tcttcggcgg aacggaaaat ctggtcgttg aagtctttgc
     5821 gctgtacagg gcggttggtc gggatgatga cggtttcgag gttgtagatg ctttggaact
     5881 cgaaagcttc ggtatcggct gtgccggtca tgccggagag cttggtgtac aggcggaaat
     5941 agttttggaa ggtgatggag gcaagggttt ggttttcgcg tttgatttcc acgccttctt
     6001 tggcttcgac ggcttgatgc agaccctccg accagcggcg gccggacatc agccggccgg
     6061 tgaattcgtc cacgatgacg atttcgccgt cttggatgac gtaatgttgg tctttgtgga
     6121 agagggaatg cgcgcgcaat gccgccataa ggtggtgcat cagggcgata ttggcggcgg
     6181 aatagaggga gtcgttttct gccagcaatc ccatttgggt caggatttgc tcggcgtgtt
     6241 cgtgacctgc ttcgctcagg atgacctggt gcgccttttc atcgacccag tagtcgcctt
     6301 cgccttcttc tgtctcttgg cggacgaggt ggggcggaac ggtgttcatg atttggtaca
     6361 actggatgtt gtcgtctgcc tggccggaga taatcagcgg agtgcgcgct tcgtcaatca
     6421 agatggaatc cacttcatcg acaacggcaa aattcaattc gcgctgcact ttgtcgtatt
     6481 ggtcggtaac catattgtcg cgcaggtagt cgaagccgaa ttcgttgttc gtaccgtagg
     6541 tgatatcggc ggcataggcg ttttgacggt cgaacggctg catatctgaa ataatcacgc
     6601 ccacggtaag gccgaggaaa ttgtagagcg gctccataat gcccgcatcg cgtgaggcga
     6661 ggtagtcgtt gacggtaacg acgtgtacgc ctttgccggc cagcgcgttg agatagacgg
     6721 cgagggtggc gaccaaggtt ttgccttcgc cggtacgcat ttcggcgatt ttgccgtcgt
     6781 gcagcaccat accgccgata agctgcacgt cgaagtggcg cataccgagg gtgcggcggg
     6841 acgcttcgcg gcagacggcg aaggcttcgg gcaaaatgcc gtccaaagtc tgaccgtcgg
     6901 cgaggcgttg tttgaattcg gcagttttgg cttgcagatc agcatcgctt agggcttgca
     6961 tctgttcttc gagcgcgttg attctggcaa cggatttacg gtattgtttc agcaagcggt
     7021 cgttgcggct gccgaagatt ttcttggcaa tgtttgtcag catgaataat tcctgcgcct
     7081 gatataatag tgggcgaaaa cgcgatttta acacaagcgt ttgtgcaaag tgctaatgcc
     7141 gtctgaaata aaggcgcgtt tgcggtattt caatcttttt gggtgtatgt tttatgaatt
     7201 tggaacagtt gggcaggcgg gacgcgctgc tttccggact tttgaaacag gcgggacagt
     7261 ggcggcggtt ggatgccgcc gtgaaaaaac tgctgcctgc caacctgcac ccgcattttc
     7321 agacggcatg tatcgaagac ggtaggctga ttcttttggc ggcaaacaat atggcggcat
     7381 cgcgtttgaa gatgattgcc ccgtcggtat tgccgcagtt ggcagggctt gatgcttcga
     7441 tacgctctgt ttcggtcagg ctggtcccga aaccggaaaa accgccgaaa accaataccc
     7501 tgcatttgag taaggctgcg ctggagagtt tggattcggc ggcggcaaag ttggaaaaac
     7561 ggcatcccga attggcggag gcattggcaa acttggtcag aaggcatggc gcataaaatg
     7621 tatacgggaa tttgtgtaaa catccgttaa tattaagaag taaaggataa tgggtctaat
     7681 actaaagaaa taggttcggg gtaaaattgc cccttttaaa gtaaacgatt gtaaacttgc
     7741 agacaggctt tgatttcaaa tgaaatttgt agcaaaatgc cgccccgaaa catctgtttg
     7801 tgcaacgcgg cggaatcttt ttcaaggttt tgttaatggc ggttgcactt tgatttccgt
     7861 aaaaccgaat attattttat cgattggaga tttgttatga aaacgtatct ggctctgatt
     7921 tctgctgccg ttatcggttt ggctgcctgc tctcaagaac ctgccgcgcc tgctgccgag
     7981 gcaactcatg ctgctgaagc acccgcttcc gaagcacctg ccgccgaagc tgctcctgca
     8041 gatgctgccg aagcccctgc tgccggcaac tgtgcggcaa ctgtcgaatc caacgacaat
     8101 atgcagttca acaccaaaga catccaagtc agcaaagcat gtaaagagtt caccatcact
     8161 ctgaaacata ccggtacgca acccaaagcc agcatgggtc acaaccttgt gattgccaaa
     8221 gctgaagaca tggacggcgt atttaaagac ggcgtaggtg ctgccgatac cgactatgtc
     8281 aaacctgacg atgcgcgcgt tgttgcccac accaaactga tcggcggcgg cgaagaagct
     8341 tccctgactt tggatcctgc caaactggct gacggcgaat acaaattcgc ttgtaccttc
     8401 ccaggtcacg gtgctttgat gaacggcaaa gtgactttgg tcgattaatc cgcttaaagt
     8461 ctcaaaagac ggacagcctg ctttgtgcag gctgttttat tataaaatga ctgcttgaaa
     8521 agtgcccgtt gagaacgaaa acatgaatcc gtttgaaacc aaaagcgtta cctttgccga
     8581 accgattgaa atgctgtatg cctgccacgg caaagtgcgc cgtttttgcg gacaagtcgc
     8641 catgctgtcg gactatatcg ccgaaaacgg ctgcaatcag attgttttgc aaaccatccg
     8701 ccaaatcgcc cagtatttca acgttgccgc gccgctgcac catgaagacg aagaagaaaa
     8761 cttcttcccg ctgctgctgc aatatgcgcc gcaagcccaa gaaagcgtgg acgagctttt
     8821 gcgccaacat atcgggctgc acgacaactg ggcggctgtt tccgccgaat ttgccaaact
     8881 cgaagcagac aacgcttatg tccccgatga ggaagcgttc aaacgttttg tggcgggata
     8941 tgatgttcat ttggcgattg aagagccgct gtttgatatg ggcaacacct ttatcccaaa
     9001 agaaaaactg accgaaatcg gcgaaattat ggcggcgcgc cggcgcaaat aatgccgtct
     9061 gaaacaatcg tctcaaggat atattatgct gaccccgaaa agttgcgatt tgttcaatat
     9121 cccgtttttc caattttccc agcttaaaaa ataccagcct gaaagcattc cgcaaatcaa
     9181 ggctgactat aaagaaaact ggcagatatg gcagcagctg attcagcagg ttgccgccga
     9241 attgggtgcg ccgtttgcgc cgccgcacat cgaacgctgg tgtaacggct ggcaggttcg
     9301 cgcccatttc tttgcctact tcaaatacga acaatataaa aattccgccg cgattttatc
     9361 gattttattg aaccgccgcc gcctgagcgt cagcttggat tggcactgct acaaagccga
     9421 cgtgtcgccg attgcgctgc ccgaatacaa ccgctggttg gataattttg ataccgaaaa
     9481 atacgcttct ttcgatatgt ggcacggtgc ggaaagcgaa tacgacgact accgcaccgt
     9541 cgcgcaacaa aacgagtccg accgaaagtt gcaaaacgac gaagactttt tctgcatcgg
     9601 caaacacatc gaacgcgacg atttgggcag gcaggatgtc gcgaaatgga tagcggaaac
     9661 ggtggaagat ttgctgcctc tttacgaagc gtgtcacggc aaatgattca gaagactgac
     9721 gggtaaaaat acccaccccg tatgccaacc taacctaaat gccgtctgaa aatcacattc
     9781 agaacgtttc agatggtcta atttcaaaag tgaacaaata tgaccgaaac ccaatcccta
     9841 gaactagcca aagcgttgat ttcccgccca tccgttaccc ccgacgaccg agattgccaa
     9901 aaactgcttg tcgaacgcct gtacaaaatc ggttttgcgg ctgaagaact ccatttcggc
     9961 gacaccaaaa acatctggtt gcgacgcggc acgaaagttc ccgtcgtctg ttttgcaggg
    10021 cataccgacg ttgtaccgac aggccctgtt gaaaaatggg attcgccccc gttcgagccg
    10081 accgaacgcg acggaagatt atacgggcgc ggcgcggcgg acatgaaaac cagcatcgcc
    10141 tgcttcgtta ccgcctgtga acgcttcgtt gccgaacatc ccgaccacca aggcagcatt
    10201 gcgcttctga ttacttccga cgaagagggc gacgcgttgg acggcacgac caaagtcgtc
    10261 gatgtattga aagcgcgcgg cgagttgatt gactactgca tcgtcggcga gccgaccgcc
    10321 gtggacaaat tgggcgatat gattaaaaac ggccggcgtg gctcgctgtc gggcaacctg
    10381 accgtcaaag gcaagcaagg tcatattgcc tatccgcatt tggcaatcaa tcccgtccat
    10441 acttttgccc cggccttgtt agagctgacg caggaagtct gggatgaagg caacgaatat
    10501 ttcccgccaa ccagctttca aatttccaat atcaacggcg gcacaggcgc gaccaacgtc
    10561 attccgggcg agctgagcgt caaattcaat ttccgcttct ccaccgagtc caccgaagca
    10621 gggctgaaac aacgcgtcca cgccattttg gacaaacacg gtgtgcaata cgatttgcag
    10681 tggtcgtgtt cggggcagcc cttcctgacc cacgcgggca aactgaccga cgtggcacgc
    10741 gccgccattg ccgaaatctg cggcgtagag accgaattgt ccaccaccgg cggcacttcg
    10801 gacggacgct tcatcaaagc cattgcaaaa gaactcatcg aattaggccc gtccaatgcg
    10861 accatccacc aaatcaacga aaacgtgcgg ctaaacgaca ttccgaagct gtctgcggtg
    10921 tatgagggga tattggcgcg gttgttggct ggaaatgccg tctgaaagca gcttgcttgc
    10981 cggatatgga tgtccgacca tttgcttaga cctaacgtgg gtttgagata acacattgat
    11041 tttttatgta gagcgattta tttcaacggc aatccattcc ctctcccgtg ggagagggtt
    11101 agggagaggg catcggggcg gtaacggaaa tattgtctgt atctgccccg ttttaccctc
    11161 tccccaaccc tcccccacag gggagggagt caagttgcag gcacaccgac ggaatatggt
    11221 tgaaacccgg ccattcgggg cagtgggatt ttggtttatt tttgggaagt aaccccttcc
    11281 aaatcagggt aaacagggct gcgccttatg tgcggccctg cgtgttggag ataatagtgc
    11341 cgtcaaatta ctttcatacg cgcgaacgat tgggtgcttt gggcggcatc gtgcaacatc
    11401 ataccgcctt gtaccgcagc gtggcggtat atgaaccagc tgtgcttatc gtgcggcgcg
    11461 gatgtaagaa gttgcggtgg gcggggcgcg aattgaggat tgcggcgggt gaggttgtcg
    11521 cgttggcagg cgggcagacg tttgacgtca tcaatattcc tgattcagac gacctctatc
    11581 aggcgcaatg gattgctttc gaacaggaaa ccgtagaaag gttcgccgca caatacggga
    11641 cggcgcaggc agtgtgcgat gcggtgaagc tgccgcaccc cgggcggatg ggcgcggcgt
    11701 ttgattatgc ggcggcggtg ctgacggatg aagaagtgcc gcacaatgcg gcggaggccg
    11761 cgctttgcgg cgtgttggcg tggttgcagc atgacggcat cggttttgcc gtgtacgaag
    11821 gcgtcaacct gatgcggcaa atccgcaaac tgattaccgc cgatatggct gcggacgggt
    11881 cgtcggcgat gctggcgcag cggcttaatt gcagcgaggc cgcattgcgg cggcggacat
    11941 cggcgggaat gtcggcgacc acccagtgca tccagcccag tccggtcggc gcgtctttat
    12001 cgtaaacggt caggacgaaa cttttgtccc ggcgggcgga tttttccacg acagcgcggg
    12061 cgaagcattg tcgcctgaac agcaaaagcc gtaaggcgcg ctcaaaagct ggttttgcgt
    12121 gaagccgccg tcttcggacg ggttgtcgaa ttgcagcgtg aatgcgcccc cggcaaaggc
    12181 agcggttgaa aaagccagca gtacggcgga gaggatgagg gtttgcatag atgattcctt
    12241 tgcttgatga ataagtggat tcattatagg ctgtcaacgg cggcaggaat gtgccgaaac
    12301 ggttggatga tgtgccagaa tgcgcaaatg caaaaatcgt ggtttcagac gaccctttgc
    12361 aggtcgtctg aaatttttca tggggcggcg ctttatgtgt cgtcctgtgt gttgaaacat
    12421 tcctttctct gatgctttag aaatatcgaa tatttaggta ttgccgcaac ctaaccctca
    12481 agatttacgt catagcccgt tcctgctccg gcgtgcggga atggcaaaaa ttggggattt
    12541 tgccgtcaaa tctatgatag ggatacggag caaacaaatg attaccctgc cttccttaaa
    12601 caatctgccg tcaaaatggg atgaaatccg ccattggctc gaatctcggg tttttgagtg
    12661 cggcgttatg ccgcatccca atctgacgga atgcgaagcc aaacagttcg agcgggattt
    12721 ttgggacaat atcggctgcg cgcctgaaga atatgtccgc atccgccgcg ccatccgtct
    12781 gttggaagcg cgttatcccg acagtttgaa cgagctggtg tgtgccgcca ttgccacgcc
    12841 tttgggcgag atgctggcgg tgttcggtag caggggtttg tgtttgctgg aatttgtcgg
    12901 acagaagcat ttggaacagg aaattgccgc cgtccaaaaa gccttgcgcg gacggtttgt
    12961 gtttcgggag gatgagcgga cgcaactttt gcggcaggaa ttggacttat acttcaaggg
    13021 tcgtctgaaa acctttgcca cgcctttgga gcagattggc accgagtttc aaaagcaggc
    13081 atgggacgcg ctcttggcga ttccttacgg cgaaacgcgc agctacaagg agcaggcgca
    13141 gcgtttgggc aaccccaaag ccgtccgcgc cgttgccgcc gccaacgggc agaacaaagt
    13201 atctatcctg attccctgcc accgcgtcat cggcagcgac ggcaaactga ccggctacgc
    13261 gggcgggttg aaccgcaaac agtttttact ggctttggaa cacggcgaag ttcagacggc
    13321 attgttttaa gcggggggag ggcagaacgg ctcatcgggc ttaaatatgg tgtgatgacg
    13381 gctgtggaaa ttcatacaac ggcaatcctt ttaaggcatc ggtcttaatg tctttctgat
    13441 tcatatgctt gtcgttttgt cgcccatttt tccaaaatta aggattgaca cattttttcc
    13501 ggacgctata atacgcccca ttccccgata gctcagtcgg tagagcgacg gactgttaat
    13561 ccgcaggtcc ctggttcgag cccaggtcgg ggagccagaa ttttgaagcc cgaacttgaa
    13621 tgttcgggct ttttcatatt tgcacgtccg gcgttcggaa tgcggcaaga taggttttcc
    13681 aacggtttcg cggtaaaatt ctgcctgtct gatttcggtt tcttgttatg tccatcaaaa
    13741 tcctgattat ttcccccagt tggataggcg actgcgtgat gacccagccc ttgttccgcc
    13801 gtttgaagga acttcacccc ggttgcacga ttgatgtgtt cgcaccgaag tggtcgatgg
    13861 cggtgttcga gcgtatgccg gaagtgaatg aaattcttga gaattcgttc ggacatggtg
    13921 cgttggagtt gaaacgccgt tggcgggtcg gtagggattt ggggcggcgc ggatacgatc
    13981 aggttatcgt gttgcccggt tctttgaaat cggcaatcat cgcgctggcg acaggtatcg
    14041 gtaaaaggac gggttatgtc ggtgaaagcc gttattttct gttgaacgat atacgcaggc
    14101 tggataagga acgtctgcct ttgatggtgg atcgatatac ggctctcgcg catccgagtc
    14161 aggaagattt tgacgggcat tcgggattcc ccgagttttc cattgatgaa cggcggcggg
    14221 aaatttctgt cgaaaccttt ggcttggata ttggaaagcc tgttctggct ttttgtccgg
    14281 gtgcggaatt cgggccggca aagcgttggc cgacaaggca ttttgccgag ttgggcaaac
    14341 attatctggc ggcgggttgg caggtttggc tgttcggttc gcaaaaagat gatgaaattg
    14401 ccgaggaaat caaccgcctt tcagacggta tgtgtgtcaa tttgtgcggc aaaaccgatt
    14461 tgtcgcaggc aatggatttg ctgtcgttgg cggacacggt cgtgtgtaac gacagcggat
    14521 tgatgcattt ggcggcagct ttgggcagga aggtggtggc ggtttacggt tcttccagcc
    14581 cgacgcatac gccgcctttg agcgataggg cgaaaatcgt cagcctgcac ttggaatgtt
    14641 cgccgtgttt taagcgggaa tgtccgctgg ggcataccga ctgcctcaac aggctgtatc
    14701 ccgagaagat tgtgcaggcg gttgaagagg cggtatgatg ggttctgttt tcgggtttat
    14761 gccgtctgac ggcggatatt gataggatgt ttatggctat tgcgaacaat aaaaaagcat
    14821 ttcacgattt tttcattgaa gaccggattg aagccggttt ggtcttggaa ggctgggaag
    14881 tcaaagcaat ccgcgccgcg cgcgtacagc ttaaagagag ttatatctat tggaaaaaag
    14941 acgcgtttta tctggtcggc tgccatatta cggctttgcc cacagcctcg acacacatta
    15001 aaccagatgc cgtacgtccg cgcaagctct tgttgaacca gtcggaaatc aacaaattaa
    15061 tcggcaaaac cgaacgtgcc ggctacacca tcgtgccgct ggatttgcat ttttcacgcg
    15121 gaaaaatcaa gatggaaatc ggtttggcaa aaggtaagaa acagcacgac aaacgccaga
    15181 gtatgaaaga ggcggattgg aaacgcgaga aacagcggtt gattaagcat acgcgctgat
    15241 tgtggaaaca gtgcttcaga cggtatgggc aaggctgccg cctgatgtgt aaacgttcta
    15301 cacctaaacc agcatccgct ttaatcagca cggcatgatg ccccaagctt tcgtcaaagg
    15361 tttgagcaat ttgatgataa attggcatta gccaagggta aagtgcggtc atttttcctg
    15421 aaatttttgt aaataacgag ggcaattata cacattttca aactggcttg acaaaatagc
    15481 aaaaagcaaa cgtttacttt taataaacaa gaaataaata gagaaaaatg tgagataaat
    15541 gacatttttt gttcagtttt atgctaaaat ccacgcccca tttattttta cttgacaaag
    15601 gaaaatttat tatgaaactc gaagcaagca agcaagcaga agtttaaaaa atcatttatt
    15661 gtaagtctat ttttttctat tctttatacc tctccgcttt tggctgttga ttacgtttac
    15721 gacaattcta aattaacaaa tgagcaaatt gatcgcttaa aaaaacttag agataaaagc
    15781 agcgaatact ggaaacaaga aacttatctt ctaaaaccga cgaatggtag ctctcagaac
    15841 tctctcaaag ttcccgcact atactctaaa acacaccaat tcaaaaacat caacaactca
    15901 aaaaagattt ctttttatga caaggaatac actgaagatt accttgttgg cttcgcccga
    15961 ggtttcgggg ttgaaaaaag aaatggggaa gaagaaaaaa cactccagca atattttaag
    16021 gactgtgtaa atactgggaa ttccaataat gataagtgtg aacaaccatc cttcggaaac
    16081 tctggcccaa gattaataaa gagcgatatt ttcgccttag ctccacaaat aaaaaatagc
    16141 catatcaaca gcgaaatttt atctgtaggt aattatatcg aatggcttcg cccaacactt
    16201 aataagcttg ctggttggga agagcatcta tattctggtt tagatccatt ccactatatt
    16261 gaggttacag acaactcgca cgttatcggg caaacgatta gtttggatga attccactta
    16321 gaaaattctc tctgggaacc ccgttgggat tctgatgttg gggagttaaa aacaacgaat
    16381 gcagacatcc gttttaatac taaaagtgaa agtttactcg taaaagaaga ttatgctggc
    16441 ggagctcgtt ttcgttttgc ttacgatccg aaagaagcga caaatgccac attaattttt
    16501 gagaaaaata taactggcac ttccgacatc atcttcgaaa atccaataga cgatttaaaa
    16561 agtctcgacg ggcatcaaat tatcaaagta aatggcacag cagataaaca cgcattccgt
    16621 ctttctggca aacaccaaaa gggaatttat acgctttctt tacaacaacg cccagaaggc
    16681 ttttttacca aagtgcaaga acgcgatgat atttcgattt atgcacaaca ggctcaagct
    16741 gccaatacct tattcgcctt gcgtttgaac gacaaaaaca gcgatatttt cgaccgcact
    16801 ttaccacgca aaggcttgtg gttgcgtgtg attgacggac attccaacca atgggtgcaa
    16861 ggcaaaacag cacctgtaga aagcaatcgt aaaggtgtgc aactcggcgg cgaagtattc
    16921 acatggcaaa acgaaagtaa tcaactttct attggcttaa tgggcggaca ggcagaacaa
    16981 cgcagtactt tccgcaatcc agatacagac aatcttacaa cgggaaatgt gaaaggcctt
    17041 ggtgcaggcg tttacgccac ttggcaccaa cttcaggaca aacagacagg tgcgtatgcg
    17101 gatagctgga tgcaatatca acgcttccgc caccgtatca acactgaaga tggtacagaa
    17161 cgttttactt caaaaggtat tactgcctca attgaagcag gttacaatgc gttattggcg
    17221 gaacacttca ctaaaaaggc aacagccttc gtgtttacct acaaccacag gcacaattga
    17281 cttatttggg ggtaaacgga aaattcagcg atagcgaaaa tgcccacgtg aatttacttg
    17341 gctcccgcca attacaaact cgggttggcg ttcaagctaa agctcaattt tcactctata
    17401 aaaatatcgc tattgaacct ttcgccgctg tgaacgcgct ttaccacaac aaacctttcg
    17461 gtgtggaaat ggacggagag cgtcgagtga taaacaataa gacggcaatt gagagccaat
    17521 taggtgtagc agtgaaaatc aaatctcact taaccttaca agccacattc aaccgccaaa
    17581 caggcaaaca tcatcaagct aaacaaggtg cgttgaattt acagtggacg ttttaagtta
    17641 agttgtaaca aaataaaatc ggcagggaaa ttcactgccg atttttttat tttaaaagtg
    17701 cggtcatttt tcgtgtaatt tccaccaatt tttaaccgca ctcacgagct cgcgctaaac
    17761 cgacaatcgg atctattgtc cctttctttg ttgtgtatgc aaatcagaaa tgccggttac
    17821 ggaagcagtt tgcccggatt cataatgtta tatggatcaa ggtgttgttt gatgcttttc
    17881 atcagggcga tttcggcagg cgtgcgtact ttgtccagcc actgtttttt gatgatacct
    17941 atgccgtgtt cggcggcaat cgtgccgttg caggcaagga cgttgcgata gactgtgctg
    18001 ttgatgtcgt tttcgtaacg atagacttca ttgctgagga tttcgggcag gaaagtattg
    18061 tagtgcaggc tgccgtcgcc cagatgtccg aagcagacga tttgtatgcc tttgaaattc
    18121 tgttccaaat ctttggcgca ccggcggaca aagtcggcaa cgcgcccgat aggaacggca
    18181 atatcgtgtt tgatgctggt gcccagtttg cgttgcgatg cggagatgtt ttcgcgcaac
    18241 gcccacatat ggatacgttc ttgttcgctt tgcgccaaca cgctgtcggt aaagcctttt
    18301 ttataaagaa attcgacaag ccgatcatca agattgctgt cgggtaatga gtcggtcaac
    18361 tcaagtaaaa tatgccattc tgaatgtgtc ggcaggggga gtttgctgaa ttcggaagac
    18421 aattcggcgg caaaacggcc gatcagctca aaactgcata ggcgttcggc aaagtgtgct
    18481 tgggtttcgg tcagcaggcg gacggcggat tcgatgtcgg gtatgccgac ccatgcggtt
    18541 gctttgtcta aggggttggc aaacagcttg agcgtggcgg cagtgataat gcccaatgta
    18601 ccttcgctac cgataaacag atggcgcagg tcgtagccgg tggtgttttt atgcagggga
    18661 tggagatggg aaaccagttc gccgttgggg aggacgactt ccaaaccgat aaccaggtcg
    18721 cgcatcgtgc cgtaacgcaa tacgttcaaa cctccggcat tgcaggcgat gttgccgccg
    18781 atttggcacg agccttcgct ggcgagactg agtgggaaca gcctgtttga ggcttcggct
    18841 gcctgttgga cggtttggag tacggaacct gcttcgacgg ttatgcagtt gtctgacaaa
    18901 ttgatgctgc ggatgcggtt gagtttggaa aggttcagca atacgccgtt ttccgatact
    18961 gccgcgccgc acaaaccagt attgccgcct tgcggcgtaa ccggaatacg gtgttggtgg
    19021 cagaaacgca taatggtttg cacgctttta acgctgagcg gctgcaaaat gatgtctggt
    19081 gcagacgtaa agcggttgcg ctggtctttt aaaagcgtcg gagaaggttc ggcaatttca
    19141 tctgccggta ggagtcggga aaattcggta tgaaggtcaa gcatgtttgt cggaagggta
    19201 gggagaagat tgaggaaggg aatctgatgc cgtctgaaaa atcaaaaaag caggatactg
    19261 aacgtatcct gcttttttgc aagcggaaaa tgcttatttt gcagcttcgg cagcaggagc
    19321 ttcagcggca ggtgcttcgg tagcggcagc ttcagcggca ggtgcttcgg tagcggcagc
    19381 ttcagcggca ggtgcttcgg tagcggcagc ttcagcagca ggagcttcgg cggcaggtgc
    19441 ttcagcagcg ggagcttcgg cggcaggtgc ttcagcagcg ggagcttcag cggctttttc
    19501 accgccgcag gctgccagaa ccaaagacaa caaagcagcg gcaaacaggg attttttcat
    19561 tttagtaacc tttagaacca atttaaatta ttgaaacaaa taggtcttta tcgaccatac
    19621 tacaaaccgg gttggaaatt catcgctgaa tttgcgtaat tatgcactat ggaataaggg
    19681 attgcaatgt cgtacagatg ggaaaaaggt ttgaatgaaa agttttctgg cgggcggatt
    19741 gataaatcct gtaaaacggt tttgctgtta aaaattcgat agggataaat attatagaga
    19801 ttaatataat ttcagtatct tgtatgattt attaaggcgg attgggaaag tcggcagaca
    19861 ggtcggaatg gttttaagaa tgttgtaaaa aagttacacc gcaataaaat aacaaagatt
    19921 tacattgaaa tatgtgctgt acagtttatg aacaggttaa cggtaatcgg ccggcgcggc
    19981 ggttgtgtta gaatgcgcct gtaattaatc tgctgttcct gaaaggacaa atatggctat
    20041 cgtgaaaaat tccgtggttt cgctgcatta tgagatgtat gatgccaaca atcagctttt
    20101 ggacaaaacc gaagaaccga ttgcgtatct gcacggcggt tacgacggca ttttcccttt
    20161 ggtggaagag gcgttgcacg gtaaggatgc cggcgatacg gtcgatgtgg cgctgtcgcc
    20221 cgacgatgcg ttcggcgagc aggatccgga gttggtccgt atcgaagatg cgggcgtgtt
    20281 ccctgttgaa gtcgaagtcg gcatgatgtt tgaagccgac gatcctgaaa ccggcgatgt
    20341 tgtcgtctat cgtgtaaccg atgttgccga cggcaaggcg gtggtggacg gcaaccatcc
    20401 tttggcaggc atgaaaatcc gctttaaggc tacagttgaa agcgtgcgcg atgcatccga
    20461 tgaggaaatc gcacacggtc atgtccacgg cccgcacggt catcaccacc actgattgcc
    20521 ggcagcttgg aggtatctga tgccgtctga aaagggtttg ttccgtttca gacggcattt
    20581 tgtttgatgg atggagaagg tatgtcggtt gggcattatg agaattttcc cgtcggttcg
    20641 ctgattttgc cgcgcaggtt gaggaagccg gttcacgcgg tgtacgcgtt tgcacggacg
    20701 gcggacgata tggcggacga gggcagtatg ccgtctgaag ccaggttggc ggggttggag
    20761 ggtttgcagc gcgagttgga cgtgttggca tcgggcggcc ggtcggcgca tcctttgatt
    20821 gcacgattgg atgccgaggc ggttgtgccg ttcggtttgg atttgcagcc gttttatgat
    20881 ttgctttctg cattttctca ggatgtggtt aaaacaaggt atggaaattt cggcgagctg
    20941 gccgattatt gtcggcgttc cgccaaccct gtcggacgca ttatgctggc tttgtacggg
    21001 aaaacggatg cggtgtgcgt ggcgcaaagc gacggcatct gtacggcttt gcaactggtg
    21061 aatttttggc aggatgtggc tgtggattgg caaaaaggca gggtttatat tccgcaggac
    21121 gatttgttga aattcggtgt ttctgaggaa cagatcgcgg caggacgggc ggatgcggcg
    21181 tttcagcggc tgatggcgta tgagtgtcgg cgtgcgttcc gtatgctgaa ggcgggttcg
    21241 cctttggcaa gggaactgaa tgggcgtatc ggtttggaac tccgtatgat tgtgttgggg
    21301 gcgcagttga ttttgcagaa actggacgcg tgccgatacg atgtgtttgc acaacgcccc
    21361 gttttggata agaaggattg gttgattatg ctgaaaaagg cgttgtggaa atgattgggg
    21421 gaaaatgaat gccgtctgaa aggggttgtt ccgcttcaga cggcattttg caggcggttg
    21481 gtatggtcag gcgatttcat gcgggttcaa tctggcttga tcttctgcac ccaagccgat
    21541 gagtttgcgc agtcgggtca tgtagaaatt gacatccaaa ccggtgccgt agcgttgcgc
    21601 cgtccacagt gtttccgcca gtgcctccat catttcgtgt tcggcttcca gccagccgcg
    21661 tttggcgcac agggtgtcgt ggattgcgcg tatgccgtgc ggctggtcta tgcccgcctg
    21721 ttcttggacg gacagatgca gcgacatatg caggaagggg ttgctttcgc cgttttcggg
    21781 cagccagtcg gtgtccagat ggtcttcgat gcgttcgaga tagcggtggt attcgggatg
    21841 ggcttcgaca atgcggaggg ctttctgttc cagtgcgctc agttgcagcg gattgagccg
    21901 ctgctgccac acgcgggcga aaaagcggcg gacatcgtgg gtattgacat cgtacatggc
    21961 gggttcgata agatggttat ggaaataggt tcggataatg gcgggtttta cataaattaa
    22021 atcccaccgt caaccttacc atttataatg cagacaaatt atttgtctga aacggattcg
    22081 attatgaaaa aactgatttg cctgttcgcc gtatttttga tgttgtgcgg acgagctttc
    22141 gcgctggatg cgaacgatct gctgccgccg gaaaaggcat tcgtgccgga gcttgccgtt
    22201 gccgacgacg gtgtgaacgt ccgtttcaag attgccgacg gatactatat gtatcaggcg
    22261 aaaatcgtcg gcaagaccga tccggcggat ttgttgggac agccttcttt cagcaagggc
    22321 gaagagaagg aagacgagtt tttcggcagg cagacggttt accatcacga agcgcaggtt
    22381 gcctttcctt atgcaaaggc tgtcggcgaa ccgtataaat tggttttgac ctatcagggc
    22441 tgtgccgaag ccggcgtgtg ctatccgccc gtggataccg agtttgatat ttccggcaac
    22501 ggcacttacc atccgcaaac cgacgaaccg gcatccgcca aagaccgctt tttgcagcct
    22561 tcctctcaaa acggcagcgg ggcgttgccg cccccgaagg gggatgaggg cggcgacagc
    22621 cgtttcaagc tgtcttggga tacgctcaac gccaatcttt tggcgttttt tctcgccggt
    22681 ttgggtctga gttttaccgc ctgtatgtat cccctgttgc cgattgtttc cagtattgtg
    22741 gtcggcgaca aaaaggcggg caaggcgcgg gcgtttgtgc tgtccgtcgt ttatgttcag
    22801 ggtttggctc tgacttatac gctggtcggc attgttgccg gactgacggg cgcgctgctg
    22861 accgtatggt tgcagcaggc ttgggtggtg ttggcggcat cggctttaat ggtcgtcttg
    22921 gcactgtcta tgttcgggct gttcaacatc cagcttccca acgccgtgca gtcgtatttc
    22981 caaaaccaga gcagcaggct ttcaggcggt aaaatcgttt ccgtctttat tatgggcata
    23041 ttgtccgcgc tgattgtcgg gccgtgcgtc gccccgccgc tggcatttgc tttgggctac
    23101 atcggtcaga cgggcgatgc ggttttaggc ggtttggcac tttacacttt ggcgttgggc
    23161 accggcgttc cgctgattgc catcggcacg ttcggcgggc atatcctgcc tagggcaggc
    23221 gattggatga atgccgtcaa atacgctttc ggctttatcc tgcttgccgt cgccgtttac
    23281 ctcgccacgc cgcacttgcc ctattatctc gtcgtcgcgc tgtacacgct gctgatgctg
    23341 gttcctgcct gtatgctgct ggtcaacgga cgcaggcaga aacgccgtcc gaaagctgtg
    23401 gcattcgcat tgggcggcat attgctgata ggcggcgcgt ggttcggctg gcagggcgca
    23461 aacggcaaaa cgaccgcgct gcaccatttc ctgaccctca atccaccagc cgaagcaggc
    23521 aaatcttcgg aacacggcaa aatgtttgcc gatactgccg cgctgaaggc agcgatggat
    23581 acggcgttga aagaacatcc cgacaaaccc gtcgttttgg atttttatgc cgactggtgt
    23641 atttcctgca aagaaatggc ggcatacacg ctcaatcagc cggaagtgca tcaggcggtt
    23701 gatatggaac gctttttcca aatcgacgta accgccaaca cgcctgaaca tcaggcgttg
    23761 ttgaaagaat acggtctgtt cggaccgccg ggcgtatttg tcgtccgctc cgacggcagc
    23821 cgcagcgagc cgctgctggg ttttgtcaaa gcagacaagt ttatcgagtg gtatgaacaa
    23881 aaccgctgat tccgatgttt caaatgccgt ctgaagttct cagacggcat ttttgtcaga
    23941 aaggaaaacg gcaatatcca ctatacacac ggaagcagtc taaagatgtg aaaaccgtgt
    24001 gtataagtta gtttgcgtat tgaaattaaa agataaaaat acgatgatta aaaaataggc
    24061 ataagaaaag ataaacacag ccggtgtttg ccggtgtgtg tatcttttgt ggaaaaacat
    24121 cttaatgtca tgtattttat ggaaaataat aacatgatta aaaaatagtc aatcattaag
    24181 gttcaggtaa gcggctgcaa ccgtcaatgt ctgaaaagag gcagtgctgt ttaagcatgg
    24241 aaagccggat aaaaaaaact gcctccgttt ccggaggcag ttcgtgtgcg gataaaacct
    24301 tacagaccgc tcaaacgggc agtgtcgtgg gcaatcatca gctcttcgtt ggtcggaatg
    24361 accacggcaa cggctttgct gtcggcggtg gtaatcacgc cggcgttgcc gaagcgggct
    24421 ttcaggttgg cttcttggtc gatgttcaga ccgaggaagc ccaagtagcc gatcacgcgt
    24481 tcgcggatga tgtcggagtt ttcgccgatg ccgccggtaa agaccagtgc gtccaaaccg
    24541 cctgcggcaa ccgccatact gccgatgtat ttggcaaggc ggtagataaa catatccaag
    24601 gccaatttcg cgcctttatg ccccttggcg gcttcttctt caatggtacg gcagtcgttg
    24661 gacaggccgg aaatgccgag cagaccggat tttttattca gcatttcagt gatttgggcg
    24721 atggtcatat tggcgttttc ggcgaggaag ccgaatacgg aaggatcgat gtcgccgctg
    24781 cgcgtaccca ttaccagccc ttccagcggg gtcaggccca tactggtgtc gcgcgattcg
    24841 ccgttggcga cggcggtgat ggacgcgccg ttgcccaagt gggcaatgac catacgcagg
    24901 tcttttttgt ctttgccgag gaagcgcgcg gtttcgtcgg cgacgaagcg gtagctggta
    24961 ccgtgcgcgc cgtaacggcg caggccgtat ttttcataca actcctgcgg aacggcgtat
    25021 ttgtaggcga cttcgggcat ggtttggtgg aaggaggtat cgaatacgac gacgttgggc
    25081 aggcctttga aaatgctttg cgcggcacgc aggcccaaga ggtgggcggg gttgtgcagg
    25141 ggggcgagcg ggatgcattt ttcgatgccg gcaatgactt cgtcgtcaac gaggatggat
    25201 tcgctgtaca gttcgccgcc gctgacgacg cggtggccga tggcgccgat gcggctgtcg
    25261 aggccgtggg ctttgagttc ttccatcagg gcttcgaccg cgccggtgtg gtcgggatgt
    25321 gcggacagat cgactttgtg tttttcgccg tttactttga atgtgatgta ggcatcgggc
    25381 aggttgagtt tttcggcaag gcagctcagc aggacttcgc cgctgccgtt atccaggacc
    25441 gcgcctttga gggacgagct gccgcagttc aaaaccaaga tcaatttttg ggacattttc
    25501 ttactccgga aagtttcaga cggcattgga atcggacacg gatactaacc ggattcgtgc
    25561 cgaatccgtt ttgccttcct gggcgggaaa gtagtggggc cgtctgaaaa ggttgataaa
    25621 agaagcaggc tattctagca aaaaatcttt gcaattgctt ggcttaatcg ggcgtttgcg
    25681 tgaaaatggc ggaagtcggg cattttgacg gaaaaaaggg ggatatgcgg tttgccggtg
    25741 ttttcggctg ggtgagggag aatttctttg cattcttccc gtccaaactg gtacattcgc
    25801 cctgttttta gttggtgtcg tctgaaaaaa ggcgggtggg aaatttatgc attatttcag
    25861 tattcatgac caatcgggac ggcatatagg ctttttcatc ctgcttgccg acgatgagtc
    25921 ggaggcgcgg ccgcagagcg gacgttttgc cgttaaattg gaagatggag cgggcaacca
    25981 tgtcctgtcg ccgtttgccc agacggaaat cccccaatat tggcgcgtgg tcaaagaccg
    26041 tatcgagctg ttttttgacg aagcccccgt cggaactttg cgtaacgaat atctgaccgt
    26101 atcggggcag acttttgttt tgaacgattt gacagggaat atatgatgga aagtatgttt
    26161 atattggtgc ccatcagcat tattttggcg tttgtcatcg gctggttttt ttggtggtcg
    26221 ggcaaaaacg ggcagtttga cgacttggaa gggccggcgc accgcatcct gatggatgat
    26281 gattcgacaa gtaaactgat tgaaaaagag aaggaaaacg atgtacgcta aaaaaggcgg
    26341 tttgggactg gttaaaagcc gccgtttcgc acctcttttc gctacgcagt ttctcggcgc
    26401 gttcaacgac aatgtgttca aaaccgcgct gtttgtgatg attgggtttt acggtttggg
    26461 gcaaaacggc ttcctgcctg ccggacagat gttgaacttg ggcgcgttgt tgtttatttt
    26521 gccgtatttc ctgttttcct cgctgtcggg gcagttgggt aacaaattcg acaaggccgt
    26581 tttggcgcgt tgggtcaagg tgctggaaat gatcattatg gcggtggcgg catacgggtt
    26641 ttatatccgg tctgccccgc tgcttttggc gtgtctgttt tgcatgggcg cgcaatcgac
    26701 gctgttcggg ccgctgaaat acgccatcct gcccgattat ctcgacgaca aagagttgat
    26761 gatgggcaac agcctgattg aatcgggtac gtttgtcgcc atcctgttcg gtcagatttt
    26821 gggaacggcg gtggcaggtg taccgcctta tattgtcggg atactggttt tgctggtcgc
    26881 cgtaggaggc acggtcggca gcctgtttat gccgtccgta cccgccaagg ctgccgatac
    26941 acaaattgag tggaatattg tccggggtac aaaatccctg ctgcgtgaaa cggtgcggca
    27001 caagcccgtt tttaccgcca ttatcggtat ttcgtggttt tggtttgtcg gcgcgattta
    27061 taccacgcaa ctgccgacct ttacccaaat ccatctaggc ggcaacgaca atgttttcaa
    27121 cctgatgctt gccctgtttt ccatcggtat tgccgccggt tcggtactgt gtgccaagtt
    27181 cagcagggaa cggctgatgt tggcttgggt aacggttggt gcgttgggtt tgacggtttg
    27241 cggtctggtt ttggtgtggc tgacgcacgg acaccgtttt gaagggctga acggcatttt
    27301 ttggttttta tcgcaaggat gggcataccc cgtgatggcg gtgatgacgc tgatcggctt
    27361 tttcggcgga tttttctccg ttccgctcta tacctggctg caaaccgcca gcagcgagac
    27421 tttccgcgcc cgcgccgttg ccgccaacaa tatcgttaac ggtattttta tggtttccgc
    27481 cgccgttttg agcgcggtgt tgctgttttt gtttgacagc atttccttgt tgtatctgat
    27541 tgtcgccttg ggcaatattc cgttgtcggt atttttgatt aagcgcgaaa ggcggttttt
    27601 aggcgcggcg gcaatcagga aagaaccttg aatgccgtct gaaaagtttg tccccgcaat
    27661 acacaatacg cctgcccaat gttgtgaaca cactgtgaat aagtgggttt gtgcattgaa
    27721 atgaaaacag aaaaatacgc tgcctaaaaa atagtcagca gatggaaaat ttgaagaaag
    27781 aagcagacat gattcaacgc caaatcatcc tcgataccga aaccaccggc ctgtatgccg
    27841 atggcggcga ccgcttggtt gagtttgccg gtctggaaat ggtcaaccgc caaatgaccg
    27901 acaaaaacct gcacctttat gtccaccccg agcgcgatat gcctgaagag gcggcgaggg
    27961 tgcacggtct gacgatagag gttttggaag ggaaaaacgc accgcctttt gcagaggtcg
    28021 gcaggcagat tgccgatttt ctgcgcggtg cggagttgat tatccacaat gccaaattcg
    28081 atgtgggctt cctcaatatg gagttccgcc ggatggggct gccgactgtc gaggaactcg
    28141 gctgtaccgt taccgatacc ctggcgatgg cgcgcgaaat gttccccggg cagaaagcca
    28201 gcctagatgc cctgtgcaac cgtttttccg tcgaccgcag caagcgcgtc ctgcacggcg
    28261 cattgatcga ctgcgaactt ttgggcgaag tctatcttgc tatgacgcgg cggcagttcg
    28321 acctgatggg ggaggccgca gaggaaaaaa tggaaaccaa acccgtggtg cataccgaaa
    28381 ccaaacgcag cggtaaactg aaagtcatca gggcggacga aaacgagttg gcggcgcacg
    28441 ggcaatattt ggacggcttg ggcgaagcct gtatctggcg caaggaagcc gtgccgtctg
    28501 aaaacggcgg gacggacgca tgaagcgcaa aaatatcgcg ctgattcccg ccgccggcat
    28561 cggggcgcgt ttcggtgcgg acaaacccaa gcaatatgtc gaaatcggaa gcaaaaccgt
    28621 tttagaacat acgattggga tttttgaacg gcatgagggc gtcgatttga ccgtcgttgt
    28681 cgtctcgccc gaagacacgt ttgccgataa ggttcagacg gcatttccac aggttcgggt
    28741 gtggaaaaac ggcggacaga cccgcgccga aaccgtccga aacggtgtgg caaaactgtt
    28801 ggaaatcggc ttggcatcgg cggatgacaa tattctggta cacgatgccg cgcgctgctg
    28861 cctgccgtct gaagctttga cgcggttgat agaacaggcg ggcaacgctg ccgaaggtgg
    28921 gattttggca attcccgttg ccgatacgct caagcgcgcg gacggtggga acattagtgc
    28981 aaccgtcgag cggacgagcc tttggcaggc gcaaacgccg cagcttttcc gcgccgggct
    29041 gctgcaccgc gcattggctg cggaaaactt ggacggcatt accgatgaag cgtccgccgt
    29101 ggaaaaattg ggtgtgcgtc cgctactgat acagggcgac gtgcgcaatt tgaaactgac
    29161 gcagccgcag gacgcataca tcgtcaggct gctgctcgat gccgtctgaa cttcaattca
    29221 aaacatcagg aacacaaacg atgacgaaca tccgtatcgg acagggctac gatgtccacc
    29281 aactgaccga aggcagaaag ctgatacttg gcggagttga aatcccattt gaaaaagggc
    29341 tgctcggaca ctccgatgcc gatgcgctgc tgcacgccgt taccgacgcg ctgctcggtg
    29401 cggcaggttt gggcgatatc ggcagccatt tccccgacac cgccgcagag ttcaaagatg
    29461 cggacagccg cgtccttttg cgtgcggcgt atcaaagcgt tcaggcgcag ggttggcagg
    29521 cggtcaatgt cgatacgacc gtgattgcac agaaacccaa actcgcgccg catattccgc
    29581 aaatgcgtgc caacatcgcc gccgatttgg gcatagacat ttcgtgcgtc aacatcaaag
    29641 gcaaaaccaa cgaaaaactc ggttatctcg ggcgcatgga aggcatagag gcgcaggcgg
    29701 cggtattgct ggtacggatt tgaacgcggc gggcaggacg tgcaaaaaaa tgttaagatg
    29761 acggctgttt gttgatacgg tttcccaaag gacaaaaata tgacgacaca agacgaactc
    29821 aagcgcatcg ccgccgaaaa agcagtcgaa ttcgtacccg aaaacgaata catcggcatc
    29881 ggtaccggtt cgaccatcaa ctttttcatc gaagccttgg gtaagagcgg caaaaaaatc
    29941 aaaggcgcgg tatctacttc caaaaaatcc agcgaactgc ttgcccaata tgatattccc
    30001 gtcgtttccc tgaacgaagt atcggggttg gcggtctata ttgacggtgc ggatgaagtg
    30061 aaccacgccc tgcaaatgat taaaggcggc ggcggcgcac acctcaacga aaaaatcgtc
    30121 gccagcgcat cagaaaaatt cgtctgcatt gcggatgaaa gcaaatatgt ttcacgtttg
    30181 ggcaaattcc ctctgcctgt ggaagtcgtc gaaagcgcgc gttcccttgt ttcgcgcaaa
    30241 ctgctcgcta tgggcggaca gcctgaattg cgtatcggct acaccacttt ttacggcaac
    30301 caaatcgtcg atgtccacgg cttgaatatc gacaaaccgc tgacgatgga agacgaaatc
    30361 aacaaaatca ccggcgtact cgaaaacgga atattcgccc gcgatgccgc cgatgtgttg
    30421 attttgggga cggaagaggg tgcgaaagtc atctatccct gccaagggta atcctgacgt
    30481 taagatgccg tctgaaatcc gattccgata tgttcagacg gcatcggcat ttgaaaaata
    30541 tgcagattaa aaaaatcatg aaatggtttc ccgtcgccct gtcgcttttg ggtgcgttgg
    30601 gttatacggg gtacggcagc gaggcggtgc ggacggcggt tgccgtactc gacgtactcg
    30661 gcgcggcagg ggacgcgggt tccgacgcgc ccgcccgccg acgggcatcg gcgaaatccg
    30721 gccaccgcta cacaggcacg gtgtccaaag tctatgacgg cgacaccctt cacgttatcg
    30781 acggcgacgg cgcgaaacac aaaatccgga tggcgtatat cgacgcgccg gagatgaaac
    30841 aggcttacgg cacgcgttcg cgcgacaacc tgcgcgcggc ggcggaaggc aggaaagtca
    30901 gcgtgcgcgt gttcgatacc gaccgctacc agcgcgaagt ggcgcaggtt tctgtcggca
    30961 aaaccgattt gaacctgatg caggtgcagg acggggcggc gtggcattat aaaagttatg
    31021 ctaaagaaca gcaggataag gcggattttg ccgactatgc cgacgctcaa attcaggcgg
    31081 aaagggaacg caaaggattg tggaaagcta aaaatccgca agcgccgtgg gcgtaccgcc
    31141 gagcaggcag gagcggcggg ggcaataagg attggatgga tgccgtgggc gaatggttgg
    31201 gcatttggta aagacagcgc acggctttga acggcaaatg ccgtctgaaa accgcgcgcc
    31261 ctgcattttt gcggagacgc gcaaccgccg tctgccgcga taaaaaagtt caggcggcgg
    31321 tttttacggt aaaatggcgg attattttgc aacggcggca gttccgccgg tttttgatac
    31381 ggacggtata tggattttcg ttttgacatt atttacgaat accgctggat gttcctttac
    31441 ggcgcactga cgaccttggg gctgacggtc gtggcgacgg cgggcggttc ggtattgggt
    31501 ctgttgttgg cgttggcgcg cctgattcac ttggaaaaag ccggtgcgcc gatgcgcgtg
    31561 ctggcgtggg cgttgcgtaa agtttcgctg ctgtatgtta cgctgttccg gggtacgccg
    31621 ctgtttgtgc agattgtgat ttgggcgtat gtgtggtttc cgtttttcgt ccatccttca
    31681 gacggcattt tggtcagcgg cgaggcggca atcgcgctgc gtcgcggata cgggccgctg
    31741 attgccggtt ctttggcact gatcgccaac tcgggggcgt atatctgtga gattttccgc
    31801 gcgggcatcc agtctataga caaaggacag atggaggcgg cgcgttcttt ggggctgacc
    31861 tatccgcagg cgatgcgcta tgtgattctg ccgcaggcat tgcgccgcat gctgccgcct
    31921 ttggcgagcg agttcatcac gctcttgaaa gacagctcgc tgctgtcggt cattgctgtg
    31981 gcggagttgg cgtatgttca gaatacgatt acgggccggt attcggttta tgaagaaccg
    32041 ctttacaccg tcgccctgat ttatctgttg atgacgactt tcttaggctg gatattcctg
    32101 cgtttggaaa aacgttacaa tccgcaacac cgctgattgg agtcatatca acaaaaagct
    32161 gcccgggtat tcatttgagg cagctttttt gtggcaaatt tat
//
LOCUS       DAWHRS010000006        30210 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWHRS010000006 DAWHRS010000000
VERSION     DAWHRS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.006"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(93..932)
                     /locus_tag="WIB82_000195"
     CDS             complement(93..932)
                     /locus_tag="WIB82_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ3841567.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="WIB82_000196"
     tRNA            1217..1292
                     /locus_tag="WIB82_000196"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1357..2550)
                     /locus_tag="WIB82_000197"
     CDS             complement(1357..2550)
                     /locus_tag="WIB82_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ3841568.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVRRAETARAATPVPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFESAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2556..3659)
                     /gene="trmA"
                     /locus_tag="WIB82_000198"
     CDS             complement(2556..3659)
                     /gene="trmA"
                     /locus_tag="WIB82_000198"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ3841569.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     TVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            3802..4902
                     /gene="aroC"
                     /locus_tag="WIB82_000199"
     CDS             3802..4902
                     /gene="aroC"
                     /locus_tag="WIB82_000199"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ3841570.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRRETDQVEILSGVFKGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            4994..5419
                     /locus_tag="WIB82_000200"
     CDS             4994..5419
                     /locus_tag="WIB82_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ3841571.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETLSVEAEAAESSAAE"
     gene            complement(5493..7478)
                     /gene="parE"
                     /locus_tag="WIB82_000201"
     CDS             complement(5493..7478)
                     /gene="parE"
                     /locus_tag="WIB82_000201"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ3841572.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     ASDEALGGFATAIDVQIHEDGSLSVHDNGRGIPVGLHPEEGVSVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7545..8069)
                     /locus_tag="WIB82_000202"
     CDS             complement(7545..8069)
                     /locus_tag="WIB82_000202"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ3841573.1"
                     /translation="MLDREGYRPNVGIILINNRNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQSSDNR"
     gene            8235..9530
                     /gene="serS"
                     /locus_tag="WIB82_000203"
     CDS             8235..9530
                     /gene="serS"
                     /locus_tag="WIB82_000203"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ3841574.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDTLEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMNQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9608..10606)
                     /locus_tag="WIB82_000204"
     CDS             complement(9608..10606)
                     /locus_tag="WIB82_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ3841575.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGKYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10731..11807)
                     /gene="prfA"
                     /locus_tag="WIB82_000205"
     CDS             complement(10731..11807)
                     /gene="prfA"
                     /locus_tag="WIB82_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ3841576.1"
                     /translation="MKPSILEKLQQLSDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLEEITNALIAEHQAELLAAMGD"
     gene            complement(11912..12682)
                     /locus_tag="WIB82_000206"
     CDS             complement(11912..12682)
                     /locus_tag="WIB82_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ3841577.1"
                     /translation="MNFTDTHCHLADPALRENLPHILTAARKVGVKRFIVPATRPQDW
                     QDVADLAEMPSEHGQIRIALGIHPWFSDGIAERDFVRLETMLAHYPQAWVGEIGLDFY
                     DKTQTSQQRERQIQVFVRQLEIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12690..13685)
                     /locus_tag="WIB82_000207"
     CDS             complement(12690..13685)
                     /locus_tag="WIB82_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ3841578.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVSQALSPFSDGLDFE
                     WHVCNPLIDSSSVTLQHWRDWLDIIADKLPSCDGILILHGTDSMAYTANLLALALQGL
                     GKPIVLTGSQWPYAAENSDAPRNLSTAVAAFSLKLKQTVIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGTLAEWDETRGWHNLRLPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVRDFARQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSAGIINGGKCNLETATALMTLAVSQGWGKEDVYKELVRLKLV"
     gene            complement(13967..14650)
                     /locus_tag="WIB82_000208"
     CDS             complement(13967..14650)
                     /locus_tag="WIB82_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ3841579.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFVILGIGATVVAWIWWKKRQRIQFYRSKLKEKRAQRKAAKA
                     AKKAAQSKQ"
     gene            complement(14839..16173)
                     /gene="glmM"
                     /locus_tag="WIB82_000209"
     CDS             complement(14839..16173)
                     /gene="glmM"
                     /locus_tag="WIB82_000209"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ3841580.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVVDTANGAGYGVAPKVFHELGAQVVSIGDEPDGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRSWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16303..17154)
                     /gene="folP"
                     /locus_tag="WIB82_000210"
     CDS             complement(16303..17154)
                     /gene="folP"
                     /locus_tag="WIB82_000210"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ3841581.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNARTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMQGLPENMQINPKYQ
                     DVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGF
                     PLLIGVSRKRMIGELTGEADAAERVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGINL"
     gene            complement(17294..18187)
                     /locus_tag="WIB82_000211"
     CDS             complement(17294..18187)
                     /locus_tag="WIB82_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ3841582.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18278..20392
                     /locus_tag="WIB82_000212"
     CDS             18278..20392
                     /locus_tag="WIB82_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ3841583.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDNPKRKASVNRIIIENSTVRLDF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20456..21934)
                     /gene="ubiD"
                     /locus_tag="WIB82_000213"
     CDS             complement(20456..21934)
                     /gene="ubiD"
                     /locus_tag="WIB82_000213"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ3841584.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(21954..22469)
                     /locus_tag="WIB82_000214"
     CDS             complement(21954..22469)
                     /locus_tag="WIB82_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ3841585.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22476..22955)
                     /locus_tag="WIB82_000215"
     CDS             complement(22476..22955)
                     /locus_tag="WIB82_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841586.1"
                     /translation="MYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAALFITLFFA
                     AVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEKAVRYTRR
                     VTQVWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(23190..23435)
                     /locus_tag="WIB82_000216"
     CDS             complement(23190..23435)
                     /locus_tag="WIB82_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ3841587.1"
                     /translation="MTEQEIYRLLRDTLTELFEIEPERITPDTNLYEDLEIDSIDAID
                     LIDRIKRETGRKLQAEDFRNVRTVNDVVQAVLKIQAG"
     gene            complement(23447..23707)
                     /locus_tag="WIB82_000217"
     CDS             complement(23447..23707)
                     /locus_tag="WIB82_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ3841588.1"
                     /translation="MNDLENQIKQLIIDSLALEDITAADIGSEDALFGDGGLGLDSVD
                     ALELGLAVQKHFGFRLDGEQENLREHFANVKTLAAFVKSRQA"
     gene            complement(23704..24462)
                     /locus_tag="WIB82_000218"
     CDS             complement(23704..24462)
                     /locus_tag="WIB82_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ3841589.1"
                     /translation="MDKLDYCRRFFATWLGFVIFGVGGIMMKLVLLPYTLNGTSGSVA
                     RQLAARRIIGTSWRLFVAYLKWSGVLEVSFKGVEKLNRPGQLILANHPSLLDVVLLVG
                     HVPKMNCIVKKDLQHNPAMSSQIKGAGYIPNEESEAMLETVKAVFDSGQSLLVFPEGT
                     RTGWDGRVKMHRGAVSLGLRYAEVITPVCIKMNPPNFKKGQPWYRIPPKRIRYEITVG
                     DDILPQDWLAEKPLPIAARRLNEYLQDYFTRKTI"
     gene            complement(24455..25210)
                     /locus_tag="WIB82_000219"
     CDS             complement(24455..25210)
                     /locus_tag="WIB82_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEZ3841590.1"
                     /translation="MPSIICRFHFDIAAWRVSGSKMRDMAQWAKWAECPDFADGLPDV
                     RPELPFLPAMQRRRLSKAARLVCDAAWDIASAHPGSPVVYASHDGEMARSFDLWLELL
                     KSHTVSPTSFGLSVHNATAGQWSILRQDMSEQTALAVCADGVETALAEAASLLEEGCG
                     SVLVLAADDPLPEGYAVSATRAPFAYALAMVLTKGTRYSLTLSASDDMPSEAGMLPEA
                     YWSGLEWVRFLLNGSRECRRVYRNREWLWQRNG"
     gene            25365..25898
                     /locus_tag="WIB82_000220"
     CDS             25365..25898
                     /locus_tag="WIB82_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
                     /protein_id="HEZ3841591.1"
                     /translation="MPVFASLPPQYMDTLLECPITNTASLLPHSGRMVLIDRITRYGD
                     DFVEAGAQVSPNHILLLDDKLPYTAFIELMAQAVGAYAGIQARKNARSVRLGFLLGTR
                     KLEIFAQSVPIGTHLLATAHMSIQDAGGMGVFDCELRWTDAPETSSETLPSDGILARA
                     SLNVYSPEHPAGTTDAV"
     gene            25926..26654
                     /gene="fabG"
                     /locus_tag="WIB82_000221"
     CDS             25926..26654
                     /gene="fabG"
                     /locus_tag="WIB82_000221"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ3841592.1"
                     /translation="MTETVLITGSNRGIGKAVAFGLAEDGFDIAVHCRSRRDEAEAVA
                     EEIRALGRNARVLQFDVSDREACREILTADIEANGAYYGVVLNAGLTRDNTFPAFSDD
                     DWDVVLRTNLDGFYNVLHPLVMPMIRRRKAGRIVCMASVSGLTGNRGQVNYSASKAGI
                     IGAAKALAVELAKRKITVNCVAPGLIDTDIIDENVPVEEILKAVPAALMGLPEEVAHA
                     VRFLMDEKAAYITRQVIAVNGGLC"
     gene            26648..27898
                     /locus_tag="WIB82_000222"
     CDS             26648..27898
                     /locus_tag="WIB82_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ3841593.1"
                     /translation="MLNTRRVAVTGIGGITAFGRDWQSIQAAFKAEKNAVKYMDWHER
                     FPELEAQLGAPIENYAPPKHWTRKQLRSMGRVSYLCVDAAEQALADAGLLGDESITDG
                     RMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVRMMPHTTAANIGIFFGLKGRI
                     IPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFPSEVYVFDSLYAASRRNGEP
                     EKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGANSDAYHISTPR
                     PDAQGAILAFQTALQHANLAPEDIGWINLHGTGTHHNDNMESRAVAAVFGNNTPCTST
                     KPQTGHTLGAADAIEAAFAWGIADRQSNPEGKLPPRLWDGQNDPNLPAINLTGSGSRW
                     ETEKRITASSSFAFGGSNCVLIIG"
     gene            27952..28027
                     /locus_tag="WIB82_000223"
     tRNA            27952..28027
                     /locus_tag="WIB82_000223"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27985..27987,aa:Ile,seq:cat)
     gene            28102..28860
                     /locus_tag="WIB82_000224"
     CDS             28102..28860
                     /locus_tag="WIB82_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ3841594.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQPPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            28861..29925
                     /locus_tag="WIB82_000225"
     CDS             28861..29925
                     /locus_tag="WIB82_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ3841595.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRDCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
BASE COUNT         7011 a         8514 c         7819 g         6866 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttagaatt ttgcaccgga ttggtttgcc
      121 atatagtcaa cagccgcttt gacttcgtca tcgctcaaac ctgcattgcc gcctttggca
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac tatcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga caggctggat acgggtctgc
      781 gttgctgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tgttgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaataat cctttgaaaa taatggttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttacg ttttacgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gagaccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttat ttcaagactt ccgcaaatgc
     1381 gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg acgcacatcc tgccggaatc
     1441 cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact tgttccacgc tcaatcctgt
     1501 gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa tcgcgatcgg ggatttgcgc
     1561 agttaagaca tcataaagtt tctgccgcat cgcacggatg cggtcgcgca tcatataaac
     1621 ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc acgtcggcgg cgatatacgc
     1681 gccgtgcgcg ggcgggctgg agtagatgcg gcggacggtg aatttgagct gtccgaacac
     1741 caaatccgct tcttccttat tcgggcaaac cacgctcagg ccgccgacgc gctcgccgta
     1801 gagcgacagg ttttttgaga aggaattgct gacgaacaag ggcaattcca tttccaccgc
     1861 tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg aagccttggt aggcgatgtc
     1921 cataaacgga atcagtttgc gcgttttgat gatgtgcaac acttcgtccc attgttgttc
     1981 cgacatatcc acgccggtcg ggttgtggca gcagggatgg aggatcagga cgctgttttc
     2041 gggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc acgccgacgg cagcagggtc
     2101 gtaatatggg taagtgccga cctcgaaacc tgcgctttca aaaatgccgc gatggttgtc
     2161 ccaagtcggg tcgctgacgt aggcgcgcgc ttcgggaaac cagcggtgca gaaagtccgc
     2221 cccgactttg agcgcgcctg agccgcccaa ggtttgtacg gtaacaatac gtccttgcgc
     2281 gagcgcgggg ttgtctttgc cgaacaacaa atgctgcacc gcgctgcggt aagtgtctaa
     2341 gccttccatc ggcaggtagg gcgacggcac aggagtggcg gcgcgggcgg tttcggcgcg
     2401 gcgcacggat tccaacacgg gcattttgcc ttcgtcgtcg aaataaatgc ctatgctcaa
     2461 attgactttt tcggggcgcg ggtcgttttt gaaggtttcg accaaactca aaatcggatc
     2521 gccgggatag tattcgatgt gtcggtacat agtccttacc tcttgctttt tccaaggatt
     2581 ttctttttca acagcacacc gctttcaata tggtgcgtaa acggaaattg atcgaacagg
     2641 gcggcacgtt caaccgtatg ggtttccgcc aaggtgtcca aattggtacg cagcgtttcg
     2701 ggattgcagg aaatgtagat gatgttgtca aactgcgaca ccagcttcaa agtttcctca
     2761 tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa atgcgtaatc cgtcaaagca
     2821 ataccgccat ccttaaggcg tttaaactca cgttttccgg tataggcttc ggtaaattct
     2881 tcagcagaca gacgggcgat tttgatgttg ccgatgcggt tggcttcgat attccattgc
     2941 gccgcgctga cggaggtttt ggagatttcg gttgccaaaa cacgttcaaa cttttcagat
     3001 aaaggaagcg taaagttgcc gtttccgcaa tacagttcga gcaaatcccc atccaatcct
     3061 tctgctgcac cgcaggccca ttcaagcatt ttctgacaca cggcggcatt cggttgggta
     3121 aaactgcctt caatttgccg ataacggaaa tcccggttgc cgaccttcaa agtttccgtt
     3181 acatagtcct gttttaagac tattttctgt cccctgctcc gcccgatgac ggaaatatcc
     3241 aactgttgct gtaacgcttg cgccgcctgc atccactcat tatcaagcct tttgtggtaa
     3301 atcatggtaa ccagcatttc cccgctgagc gtagacaaaa attcgacggc ataccaacgg
     3361 tttctcagtt cggcagattg ggcggcaacg gcaatcaatt ctggcatcag gcggttgacg
     3421 gcttcggaag ccgcttcaaa acggtcgcaa cggatcaggc ttgccccgcc agccttttgt
     3481 cctttttcaa acatggcata aaacatttca ccgccttcgt gccaaatacg gaactcggca
     3541 cgcatacggt aatgtttgtc cggagattcg tacacttccc actcaggaac atccaaacct
     3601 gcaaaaaggg ttttaaggtg gtctttttta tcttgaagct gctgcgtgta gtcgctcata
     3661 tcgctatcct gaaaaatcag accggattat aagccgattc actgcaaaac acgcaacccc
     3721 tttaccggca tataacccgt tttcctccaa cttttatgct aaaatccgcc gtctccaaca
     3781 taccgccgac agaaggaaaa tatggcaggc aacactttcg gacaactctt caccgtgacc
     3841 acgttcggcg aaagccacgg cgcaggtttg ggctgcatca tcgacggctg cccgcccggc
     3901 ttggaattaa gcgaagcgga tatccaattt gacctcgacc gacgcaaacc cggcaccagc
     3961 cgccacgtta cccaacgccg cgaaaccgac caagtagaaa tcctctccgg cgtattcaaa
     4021 ggcaaaacca ccggcacgcc catcgccctc ttaatccgca ataccgacca gcgcagcaaa
     4081 gactacggca acatcgccca aagtttccgc cccggccatg ccgactatac ctattggcac
     4141 aaatacggca cgcgcgacta ccgcggcggc ggcaggagct ccgcccgtga aaccgccgcc
     4201 cgcgttgctg ccggagccgt tgccaaaaaa tggttgaaag aaaaattcgg cacggaaatc
     4261 accgcctacg ttacccaagt cggcgaaaaa gaaatccggt ttgaaggctg cgaacacatt
     4321 tcccaaaatc ctttttttgc cgccaaccat agccaaattg ccgagctgga aaactatatg
     4381 gacagcgtgc gcaaatcgtt ggattccgtc ggcgcgaagc tgcatattga agccgccaat
     4441 gtccccgtcg gcttgggcga acccgttttc gaccgcctcg atgccgaaat cgcctacgcg
     4501 atgatgggca tcaacgccgt aaaaggcgtg gaaatcggcg caggttttga cagcgtaacg
     4561 caacgcggca gcgaacatgg cgacgaactc accccgcaag gcttcctgtc caaccattca
     4621 ggcggcatcc tcggcggcat cagcaccgga caagacatcc acgtcaatat cgccatcaaa
     4681 cccaccagct ccatcgccac cccgcgccgc agtatcgaca tcaacggcaa ccccatcgaa
     4741 ctcgccaccc acggcagaca cgacccctgc gtcggactgc gcgccgcgcc gattgcagaa
     4801 gctatgctgg cattggtttt aatcgaccac gccctgcgcc atcgcgcgca aaatgcggat
     4861 gttcaggtta atacgcccga cattaccctt tcaaacaaat aaaaatttag ccaaaacaca
     4921 gactttataa tagaataccg agcattgccg tgccgctttg acgcacgggt ttgtttagag
     4981 gaatacaacc gaaatgacac aagaaaccgc tttgggcgcg gcactgaaat ccgccgtcca
     5041 aactatgagc aaaaagaaac agaccgaaat gatcgccgac cacatctacg gcaaatacga
     5101 tgtattcaaa cgcttcaaac cgttggcgct cggcatcgat caggatttga ttgccgcttt
     5161 gccgcaatac gattccgcac tgattgcacg cgtcctcgcc aaccactgcc gccgtccgcg
     5221 ctatctgaaa gccttggcgc gcggaggcaa acgtttcgat ttgaacaacc gtttcaaagg
     5281 cgaagttacc cccgaagaac aggcgatcgc gcaaaaccat ccttttgtgc agcaggcttt
     5341 gcaacagcag tccgcccaag ctgccgccga aacgctgtct gttgaagccg aagcagccga
     5401 atcttccgca gcagaataaa tccccaaacg aaatgccgtc tgaaaaccga tttggtttca
     5461 gacggcattt tttcgtatgc ggcaatcacg gttcaaatat ccaattccgc cgtatcgcct
     5521 tcgcgttcca tccaagcgcg gcgggcggcg gcttcgcctt tgcccatcag tttgacgaag
     5581 atgtcgcgcg tctcgtcatc tgcaccttct gggatttgta cctgcaacag gcggcgggtg
     5641 tcggggtgca tggtagtatc tttgagctgg tcggggttca tctcgcccaa gcctttgaaa
     5701 cggctgatgg aataggcggt ttctttaacg ccttcttttt gcagccgctc caaaatgctg
     5761 tcgagttcgt tttggtcgag ggcgtagaat ttgcgggcag gtttgctctt accttgtgcg
     5821 ttgacatcga cgcggaacag cggcggctgg gcgacgtaga tgtgtccgtc ggcaaccagt
     5881 ttcgggaagt ggcggtagaa cagggtcagc agcaaaactt gaatatgcga gccgtccacg
     5941 tcggcatcgg acaggatggc gattttgccg tagcgcaggc cgcttaaatc gggatggtcg
     6001 ttaataccgt gcggatcgac gccgatggcg acggaaatgt cgtggatttc ggcgttgccg
     6061 aagagttggt cggggtggac ttcaaagctg ttgagcactt tgccgcgcag gggcaggatg
     6121 gcttgggtgg ctttgtcgcg ggcgagtttg gctgagccgc cggcggaatc gccttcgacg
     6181 aggaagagtt cgttttcgcg gatgtcttcg ctttcgcagt cggtcagctt accgggcagg
     6241 acggcgacgc cgctgccttt tttcttttca atctttttaa ccgaacgcat ccgcgcctgt
     6301 gcttggcgga tggcgagttc ggcgattttt ttgccgaagt ccacgttttg gttcagccac
     6361 aattccaaag ggtcgcccga tacggtggcg acgagtttca gcgcgtcgcg gttggtcagc
     6421 ttgtctttgg tttgaccttg gaactgcggg tcgaggacgc gggcagagag aacgaaggcg
     6481 gtttttccga acacgtcgtc gctttgcact ttaacgccgc gcggcaagag gttgtgcaga
     6541 ttgatgaagt tgttgactgc gttgaacacg gcttgtttca agcctgcttc gtgcgtgccg
     6601 cccagcgggg tggggatgag gttgacgtag ctttcgttgg cgcacgagcc ttcttccagc
     6661 caagtcaggg caaacgccgc tccttcgccg atgctgaaat cgccgttgtg ttcgtctgaa
     6721 atgtagtttt cgcaagagaa cagcggtacg gcttcctgcg cgtcggcaat caggtcggtc
     6781 agatagcttt tcaggccgtc ggggtaatgc caggtttggg tgtgcgcttc gtcttcgcct
     6841 ttgaccggac gggtcaggga aacgcgcaca cccggcagca gcacggcttt ggcacgcagc
     6901 aggcgttcga gttcgggaat gctgtaattc gggctttcaa aatatttgcc gtccggccag
     6961 acgcgcactt ctgtaccgct gtctttgacg gcgcatttgc ccacttgtgc caacggttcg
     7021 accacgtcgc cgccggcaaa cacgatgcgg tggattttgc cttcgcgttt gaccgttact
     7081 tcaaggcggg tggaaagggc gttggtgacg gatacgccca cgccgtgcag gccgcctgaa
     7141 aaggcatacg cgctgccgcc gtcttttttg ttgaacttgc cgcctgcgtg cagacgggtg
     7201 aatacgagtt cgactacgga tacgccttct tcgggatgca ggccgacggg aatgccgcgc
     7261 ccattgtcgt gcacggaaag cgaaccgtct tcatgaattt gcacgtcgat tgcagtcgcg
     7321 aaaccgccca acgcttcatc cgacgcgttg tcgatgactt cttggcagat atgggtcggg
     7381 ctgtcggtgc gggtgtacat accgggacgt tctttgaccg gctccaagcc tttgaggacg
     7441 gtgatgctgg attcgctgta ttggttgttt ttagccatgg gaataatctg aaagtaagaa
     7501 aaacaacgct ttcagacggc ctgaaagcgt tgcgttccgt tgttttagcg gttgtcggaa
     7561 gattggcggg cggcaaagtc ttcataactt tccataccgc gcaggaagcg ggaagagagt
     7621 tctttcaacg cccccaaata aacgtcgcgt ttgaaatcaa tcacttcgtc aacgggtgcc
     7681 caatattgat gccaacgcca gccgtcaaat tcggggtggc gggtggcgcg caggttgaca
     7741 tcgcaatctc ggccggtcag gcgcaggaga taccaaatct gcttctgtcc gcgataagag
     7801 ccgcgccatt cgcggcgtac ccagttgttc ggcacgtcat aacgcagcca gtcgcgcgtg
     7861 cggccgataa ttttgacgtg ttgcggcaaa agcccgactt cttcgtacaa ctcgcggtac
     7921 atggcggttt cggggctttc gcccggcttg atgccgcctt gcggaaactg ccaagaatgt
     7981 tcgcgcacgc gcttacccca aaagacttcg ttgcggttgt tgattaagat gataccgaca
     8041 ttggggcgat agccttccct gtccaacacg gtgtcgccct ccgttaaatt caatcttggg
     8101 attttcccac aaatcaggcg gttttgacaa atcagacggc atggcggtac gcgtgccgaa
     8161 acacgggggg atttgggaaa atatcttaaa tttggtttac aataatgtat ttcaaattat
     8221 tcgggaatca gaccatgtta gatatccaat tgctccgcag caacaccgcc gccgttgccg
     8281 aacggcttgc acggcgcggt tatgactttg ataccgcacg ttttgacaca ctggaagaac
     8341 gacgcaagtc cgttcaggtg aaaaccgaag aattacaggc ctcgcgcaac agcatttcca
     8401 aacaaatcgg cgcactgaaa ggtcagggca aacacgaaga agcgcaggcg gccatgaatc
     8461 aggttgccca aatcaaaacc gatttggaac aggctgccgc cgatttggat gccgttcaaa
     8521 aagaattgga cgcatggttg ttgagcatac ctaacctgcc gcacgaaagc gtacctgccg
     8581 gtaaagacga aaccgaaaac gtcgaagtcc gcaaagtcgg caccccgcgc gaatttgact
     8641 ttgaaatcaa agaccatgtc gatttgggcg aacctttggg tttggatttt gaaggcggtg
     8701 caaaactctc cggcgcacga tttaccgtga tgcgcggaca aatcgcccgt ctgcaccgcg
     8761 ccttggcaca gttcatgctg gatacgcaca cgctgcaaca cggctacacc gagcattaca
     8821 cgccttatat cgttgacgat acgacgctgc aaggtacggg ccaactacca aaatttgcgg
     8881 aagatctgtt ccacgttacc cgtggcggcg acgaaaccaa aaccacccaa tacctgattc
     8941 cgacagccga agttaccctg accaataccg ttgccgacag cattatcccg tccgaacaac
     9001 tgccgctgaa gctgaccgcg cattcgccct gtttccgcag cgaggcgggt tcgtacggca
     9061 aagacacgcg cggtctgatt cgccagcacc agttcgacaa agtggaaatg gttcaaatcg
     9121 ttcatcccga aaaatcatac gaaacgctgg aagaaatggt cggccatgcc gaaaacatcc
     9181 tgaaggcttt ggaactgccc taccgcgtga ttaccctgtg taccggcgac atgggcttcg
     9241 gcgcggcaaa aacgtatgac ttggaagttt gggtgccggc gcaaaatacc taccgcgaaa
     9301 tctcaagctg ctccaactgc gaagatttcc aagcccgccg cctgaaggcg cgtttcaaag
     9361 acgaaaacgg caaaaaccgc ttggtacata ctttgaacgg ctccggcttg gctgtcggca
     9421 gaacgctggt cgccgtattg gaaaaccatc aaaacgccga cggcagcatc aacatccctg
     9481 ccgcactgca accgtatatg ggcggtgttg ccaagttgga agtcaaataa gtttgcaggc
     9541 tgcctgaacg tcaaatgccg tctgaaacct gtttcagacg gcatttcctt taaactttta
     9601 aaacacgtca gccgtcggca cgaaccgcat tgccgcaatc gccggtctgt ccgacctcgc
     9661 ggatattgga cagcgtaact tccgaaatat tacccaacgc ctcttccgtc aaaaatgcct
     9721 gatggccggt aaacagcaca ttatgacaag acgacaggcg gcggaacacg tcgtcggtaa
     9781 tcacatcgtt ggatttgtct tcaaaaaaca gctcgcgctc gttctcgtac acatccatgc
     9841 ccaatgcgcc gattttccgg cgtttcaacg cctcaatcgc ggcggcactg tcaatcagcc
     9901 cgccccggct ggtgttgata atcatcacgc cgtctttcat tttgtcgaac gccgcttcgt
     9961 tcagcatata gtggttttcc ggcgtggcgg ggcaatgcag cgtgatgatg tccgaccggg
    10021 catacagctc gtctaaatcc acatatttgc cgccgatttt ttccgcttcg gggttgcaaa
    10081 acggatcgta agccagcagg ttcatgccga aaccctttaa aatccgcatg gttgcaatac
    10141 cgattttccc cgtgccgata acgcccgccg ttttgccgta catattgaaa ccggtcagac
    10201 cttccagcga aaaattcgca tcgcgggtac gctgataggc tttgtggata cggcggttca
    10261 acgtcagcat cagaccgacc gtatgttccg caaccgattc gggcgaatag gcaggcacgc
    10321 gcacgacttt caagcccaac tcttcagccg cctttaaatc cacattattg aagccggcac
    10381 aacgcaacgc cacagttttc acgccaattt gcgccaattt ttccaacacg ggccggctgc
    10441 cgtcgtcgtt tacaaaaata cagaccgctt ccgcgccttc cgccattttc gccgttttcg
    10501 catccagcat aaaatcaaaa aactccagct cgaagccgaa atgccggttg gcgcgggtaa
    10561 aatgttcgcg gtcatagctt ttcgtaccgt aaatcgcaat cttcatcaat atgtccagtt
    10621 gtcgtctatg gttgagaaac ggcataatac actgaattca aacaaatcag tagaatatgg
    10681 tggattaaaa ttgattgcat gcacggcatt tccatttcaa aacacaaaac tcaatcgccc
    10741 attgccgcca gaagctcggc ctgatgctcg gcaatcaggg cattggtgat ttcttccaag
    10801 tcgccgtcca tcacaaaatc cagcttgtgc agggtaaggt tgatgcggtg gtcggttacg
    10861 cggccttggg gatagttgta ggtgcggatg cgttcgctgc ggtcgccgct gccgatgagg
    10921 gatttgcgtt cggcggcttc tttggcttgg gcttcgcgtt tttgcgcgtc gttcaggcgg
    10981 gcggcgagaa ctttcattgc ctgcgctttg ttggcgtgtt ggctgcggcc gtcttggcat
    11041 tcgaccacca tgccggtggg caggtgggta atgcggacgg cggaatcggt tttgttgatg
    11101 tgctgaccgc ctgcgccgga tgcgcggaag gtgtcgatgc gaaggtcgac ggggttcaac
    11161 tcgatgtctt ccagttcgtc cgcttcgggc atgacggcaa cggtgcaggc ggaggtgtgg
    11221 atgcggcctt ggctttcggt ggcggggacg cgctgcacgc ggtgtccgcc cgattcaaat
    11281 ttcaaacggc tgtacgcccc gagtccgaca atacgggcga tgacttcttt atagccgccc
    11341 aattcgcttt cgttggcgga cacgatttca acctgccaac ggttgcgctc ggcgtagcgg
    11401 ctgtacatac gcagcaaatc gccggcaaac agcgcggctt cgtcgccgcc cgttccggcg
    11461 cgtatttcga tgaagatgtt tttgtcgtcg tcggcatctt tgggcagcag cagtttttgc
    11521 agttcggtat cgagttcgcc gattttggct ttggccgctt cgatttcttc ggcggcaaag
    11581 tctttcattt cggggtcgga caacatttct tcggcatccg ccaagtcgct ttgggcaagc
    11641 cgatagtttt ggaacacttc gacgacgggg gtcagttcgg cgtgttcgcg cgtgagcttg
    11701 cggtagttgt ccatgtcgga cgtggcttcg ggctgtccga gaaggtgggt aacttcttcc
    11761 agtcggtcgc tgagttgttg tagtttttct aagatagacg gcttcataat tcttccataa
    11821 caaacgccgc ctgaatgttc agacggcatc aacactggat tattataata ggttttccgg
    11881 atattcaaaa agataatctt agatggataa cctaccgtcc caacagggca tcggcattgc
    11941 gctccgttac ctttgcaatc tcttctacac aggttccgcg gatttccgca gcaatctttg
    12001 caatacccgg aatattggca ggcgtattaa tctctttttt cagcataaac gggctatccg
    12061 tttccaatac gaaatccccg tcgttcaagg ctttaagcgt atcgcgcact ttacgcgcgt
    12121 tcggattgag cagcagcgaa ccgatgccga ttttgaaacc cagtttcgtc aacacacgcg
    12181 cttcttccgc gctgccggag aaggcgtgaa cgatgccgcc ttgggcaaag cctgtctgtt
    12241 tgacggcggc ggcgatgtcg gcggtggctt tgagattatg gataatcacg cggcggcgca
    12301 gggtttgcgc aatttcaagc tggcggacga aaacttgaat ttgccgttcg cgctgctgcg
    12361 acgtttgggt tttatcgtaa aaatccaagc cgatttcgcc gacccatgcc tgcggataat
    12421 gtgccaacat cgtttccagg cggacgaaat cccgctcggc aatgccgtct gaaaaccaag
    12481 gatgaatgcc cagtgcaata cggatttgac cgtgttcgga cggcatttcc gccaaatccg
    12541 ccacgtcctg ccaatcctgc gggcgcgtcg cgggaacgat aaaccgcttc accccaactt
    12601 tccgcgctgc ggtcaggatg tgcggcaggt tttcgcgcag ggcgggatca gcgagatggc
    12661 agtgggtgtc ggtgaagttc atttcgattt cacactaact ttagtcttac caattctttg
    12721 taaacatctt ccttacccca gccttgcgat acggcgaggg tcatcagcgc ggtggcggtt
    12781 tcgaggttgc atttgccgcc gttgatgatg cccgccgagt tgcggaacgc gttgccttgc
    12841 gcgtaaacgg cggcggtttt gccttgtcgg acttggctga tgttgagcag cagtttgccc
    12901 tgccgcgcga agtctcggac ggcgcggata aaaccttcgt ctgcgggcgt gttgccgtgt
    12961 ccgtagcttt gcaggataag agcttgggcg ggaagctgtc cgagtccgtc cgcaagttct
    13021 tggacggcaa agccggggat aagcgtgcgg acagcgattt ttgcctgcgg gtcgggataa
    13081 cggattttga ggccgtctga aacggctgct gcgtcttggg acgggaggcg aagattgtgc
    13141 caaccccggg tttcgtccca ttcggcaagc gtgccgaaat gcggattgtc gaagcctgca
    13201 gcagtttcgg tgctgacttt gctgctgccg acggcgggat acagtttgcc gtcaaacgcg
    13261 atgacggttt gtttgagctt gaggctgaag gcggcaacgg cggtggagag gttgcgcggg
    13321 gcatcgctgt tttcggcggc gtaaggccat tgggaaccag tcaggacaat cggtttgccc
    13381 aaaccttgca gagcgagcgc gaggagattg gcggtgtacg ccatgctgtc cgtgccgtgc
    13441 agtatcagga tgccgtcgca tgaagggagt ttgtcggcaa tgatgtccag ccaatcgcgc
    13501 cagtgttgca gcgtaacgga ggaggaatca atcaagggat tgcagacgtg ccactcgaaa
    13561 tcgaggccgt ctgaaaaggg ggaaagggct tggctaacca gtgcggtatc ggggcgcagg
    13621 ccttcgctgc tttgggtcat gcctatggtg ccgcctgtgt agaggacgaa gattttttgt
    13681 ttcatggaca tcatcgggtc gtctgaaaat aataatacgg cttatttaac tatatttcgg
    13741 acagactggc aatttggcgg cgcggacggt tttcagacgg ccttcaaatg aaaaagcacc
    13801 cgagggctgt cgatatttga ttttccaagt agatttttat tcacgaaata ggagagccgc
    13861 aacaagctta aatcccttgt gaggttccca acacggaaga taccgctttg tggattaaaa
    13921 aatacggaaa ctattgaata tcgacaacct atttaggtgc ttgattttat tgtttgcttt
    13981 gcgcggcttt tttggctgcc ttggcggctt tgcgttgcgc ccgcttttct ttcaatttgc
    14041 tgcggtaaaa ctggatacgt tggcgttttt tccaccaaat ccaagcgaca acggtcgcac
    14101 ctatacccaa gataacaaaa atacccgatt gcaggctgtg cattttcgcc atcagccaat
    14161 cgatgttgtg cgcgccgtac tcgcccaagt aaatccaaac gggcacggaa atcagcgcgg
    14221 caagcccgtc cataatcaga aagcgcagat acgatacttt gcggctgatg ccggcggtaa
    14281 cgaaaacggc agtccgcaaa cccggcagga agcgggcgac aaacagaacc cagttgccgt
    14341 atttgtcgaa tttttcctga acctgtgcgt aacgtttcgg cgtcatgatg cgcgcaatcg
    14401 gtttgaactt gaggattttc tgcccccaaa ttcgtccggc ggcgaacatg atgccgtcgc
    14461 ccgccaacac gccgagcata ccgactgcaa acataatatg cggattggta taacccatac
    14521 ccgaaatcac gccgcctgtt accaaggtca aatcctcggg aatcggcacg ccgaaaccgc
    14581 agatgaccaa aacgaaaaac acggccgcat agccgtattc gacaaaaaag gcttccaaaa
    14641 gggcaaacat agcggatatt ccattgtcgg agataaaaag tcagaacaag ccgaaacatt
    14701 ttctgcgcga aacggcattc tatcaaagat tatgccgtct gaaagcggca aggggcagat
    14761 tcgcgccctc tgccggacgt tcagacggca tttaaacagg aaaaccgccg ctgttgaaac
    14821 ggttttgcct gattttgcct aaaggctgcc gatggcggaa gcaatacgtt ccgcaccgtc
    14881 gcgcgcccaa tccgcctgcc gcgcctccac catcacacgc acgacgggtt cggttcccga
    14941 agcgcgcaac acgacacgcc ctttgccttc gagttctttt tccacttccg ccaacacgtc
    15001 ttttgaggct tcctgccagt tttgcccttt gccgatacgg acattaatca tcgtttgcgg
    15061 atacggctgc caatcggcgc aaacggtggc gaggtcttgg ttcagcgttt gcagtgccgc
    15121 caaaacttgc agcgcggaaa taatgccgtc gccggtgttg tgtttgtcca tgcacaaaat
    15181 atggccgctg gcttcgccgc cgatgagcca gctgcgttgg ttcagctgtt ccaacacata
    15241 gcggtcgccg actttggcgc ggcagaaatc cacgccctgc tctttcaggg cgatttccat
    15301 cgccatattg gtcatgaccg tgccgaccac gccgccgatg ttgatacctt ctcgggcgcg
    15361 ggctttagca atgacgtaaa tcaggctgtc gccgtcgtaa accttaccgt ttttatcgac
    15421 catcatcagg cggtcgccgt cgccgtctaa ggcgatgccg tagtcggctt catgctgtaa
    15481 aacggcagcc tggagtgtct tggtataagt cgcaccgcat ttttcgttga tgttgtagcc
    15541 gtcgggttcg tcgccgatgc tgacgacctg tgcacccagt tcgtgaaaca ccttgggtgc
    15601 gacaccgtac cccgcgccgt tggcggtatc gacaaccaac ttcaaacccc gaaggtcgga
    15661 atggctggga aaggtggatt tgcaaaattc gatatagcgg tcgtccgcac cgctgatgcg
    15721 gcgtgcgcga ccgagacggg cggacggttg ggttttcatt tcttcgtcga ttttggcttc
    15781 gatttccaac tcgatttcat cggaaagctt cacgccgcct tcggcgaaga atttgatgcc
    15841 gttgtcggaa taggcgttgt gcgacgcgga aatcatcacg ccggcggaca ggcgcaacgc
    15901 gcgggtcaga taagccacgc cgggcgtggg cagcggtccg gtctgtacca cattcacacc
    15961 cgccgccgta aaaccggcca ccaaagcggc ttccagcata tagccggaaa tgcgcgtgtc
    16021 tttgccgatg aggacggtcg gtttctggtc ggtgtcgtgc tgcaccaaaa cctgccccgc
    16081 cgcatagccg agtttcaata cgaaatcggg cgtaatcgga aattgcccca cttcgccgcg
    16141 cacgccgtcc gtgccgaaat atttttttgc catgtgttgc tccgagaatg tgaaccgttg
    16201 tccgagatta tacagtcagt ttgtgccctg ctgtctgcac cgttgatgcc gtctgaagcc
    16261 gccccgtcct tttcagacgg catgaagtat gcgaaccgct gtttacagat tgatgcccaa
    16321 cgcttcccac accttcaacg cgtccgccgt cgccttcaca tcatgcaccc gcacgatttg
    16381 cgcgccgcgc gccacggaag ccagcgccgc cgccacgctg ccgtgtacgc gttccgccgc
    16441 gtctgcctca ccggtcagct cgcctatcat acgtttgcgc gacacgccga tgagcagcgg
    16501 aaaacccgtt tccgccatca attcgggtaa atgccgcatc agtgcgatat tgtgttgcaa
    16561 ggttttgccg aagccgaaac cggggtcgag cgtaatccgt tgcggcgcga tgcctgccgc
    16621 gacacaggtt tctgaccgtg ttttcagata acgtgccact tcaccgacaa catcctgata
    16681 tttcggatta atctgcatgt tttcaggcaa accctgcatg tgcatcaggc aaatgcccgt
    16741 gtccgcctga cgcgccagca attcgaccgc gccttcgtcg gtcaacgccg ccacatcgtt
    16801 aataatatcg atgccgccga gtgccaacgc tttttccata accaccgtgc ggcgcgtgtc
    16861 caaactgacg ggaacgcccc accccgccac ttctgccaat acaggctcaa cccgcgccca
    16921 ttcttcttca ggagaaacat aatccgcacc cggccgcgtc gattcgccgc cgatgtcgag
    16981 aatgtccgca ccctctttca aaagccgttc ggcgtgcgcc aaagcggttc gggcattttg
    17041 cgaatacgcg ccgccgtcgg aaaaagaatc gggcgtgaga ttcacgatgc ccatgatttt
    17101 cggcttgtcc aaaccgattt caaaccgtcc tgcctgccaa acgtgtcgtg ccatctgaac
    17161 tcctcccaaa ataaaaaaca gattatatgc cgtctgaaac cgtcttgtgc gcttcagacg
    17221 gcaccgctat tcgggcggca gacggcatgt tgtccgaatg tctgctccgc ctttgaatct
    17281 gccggtatgc ctgctatccg cccgactttt caaaacaggt tccgacgatt ccgcacgcgc
    17341 ctgccgcctt tgccaagccg tacaggattt cctgcggcat atcgcggttc cataatcccg
    17401 taatattcgc aatcacgggc agatggctga tttggcggac tttcacgatg gattcgacat
    17461 ccaaacggta gggatggtct ttggtatggc tcaacacgcc cgactcgccc agaatcaggt
    17521 ggcggttgcc gcgcgagaca acatattctg cggcattcaa ccaatcttcg gcagaatggt
    17581 gcttgtcttt acacagcacc aagggaatgt tcaggcttcc ggcttcattc aatacggcaa
    17641 gatcggacat cagcccgccg cccaaataca ggatgtccgc ccccgcattc aaagccgctt
    17701 cgacatggcg gacgttgcgg acgcgcacca atacgggttt ccctgcgcca tgcgccgatg
    17761 cggtctgttc cgccaaccgt ctgcaccgtc cccgcccttc atccgcactt gaagtgtcgt
    17821 ataagtttgc cgaagtgaaa aacggatcca gaaacactgc atccgcattg cgccatactg
    17881 acggttctgc ggcgatacgg acggtttccc cgccgccgaa agccacgcct ttggcggcaa
    17941 cgcggctgtc ttccgcccga ttttcccgac tgacggtttt ccatgtatcc aaaatgcgga
    18001 cggctttctc gacctccggc agcgtctgca cctccctgac gctcaaaacc ctatcgtcgc
    18061 cgattgcgcc gatgacggta cgctcgtcgc cgtgagaaat gtgttctcgc agacctctgc
    18121 tgcggataaa ggcgacaacg ccggcaatgt ccgcttcggc ggcacgcctg ctcatgacaa
    18181 taatcatatt tcctcctgac acaagaaacg gcctgcccaa aataggattt ttgcaagccg
    18241 tgttatactg tggcgtgttt tacagattgt tcgggctatg gatttattat cggttttcca
    18301 caaataccgt ctgaaatatg cggtggccgt gctgacgata ctgcttttgg cggcagtcgg
    18361 gctgcacgct tccgtatatc gcaccttcac gcctgaaaac atccgcagcc gcctccaaca
    18421 aagcattgca cacacacacc ggaaaatctc gtttgatgcg gacattcagc gcaggctcct
    18481 gccccggccg accgtcatcc tgaaaaacct gaccattacc gaacccggcg gcgaccagac
    18541 tgccgtttcc gtccaagaaa ccaaaatcgg attgagctgg aaaaacctgt ggtcggatca
    18601 gatacagatt gaaaaatggg tggtttcgag tgcggaactt gccctgacgc gcgacgggaa
    18661 aggtgtttgg aacatccaag acctaatcga caacccaaaa cgcaaagcct cggtaaaccg
    18721 catcattatc gaaaacagca ccgtccgcct cgacttcctg caggaacagc ttatcctgaa
    18781 ggaaatcaac ctcaacctgc aatcccccga ttcgtcgggg cagccgtttg aaagttcggg
    18841 catactggtt tggggaaagc tgtccgtccc gtggaaaagc agggggctgt tcctttcaaa
    18901 cggcatcggc ccgcccgaaa tctcaccgtt ccattttgaa gcttccactt cgctggacgg
    18961 acacggcatt accatttcca ccaccggcag cccttctgtc cgcttcaacg ccggcggagc
    19021 ggatgccgcc ggcctcggcc tgcgtgcaga cacttccttc cgtaacctcc acctgaccgc
    19081 ccaaatccct acgctggcac tcaggaacaa cagcattaaa attgaaaccg tcaacggcgc
    19141 atttaccgcc ggcggcgaat atgcccaatg ggacggttcg ttcaaactcg acaaagccaa
    19201 cctgcactcc ggcatcgcca acatcggcaa cgccgaaatc tccggcagct tcaaaacacc
    19261 gcgccaccag accaacttct ccctcaattc gccgctcgta tggacggaaa acaaagggct
    19321 ggacgcgccg cgcctgtatg tatcgaccct tcaggatacc gtcaaccgcc tgccgcaacc
    19381 ccgtttcatc agccggctcg acggttcgct gtccgtaccg aatctgcaaa attggaatgc
    19441 cgaattaaac ggcacattcg accgccaaac cgttgccgcg aaattcagat acacacatga
    19501 agacgcaccg catctggaag ccgccgtcgc actgcaaaaa ttgaacctga ccccctatct
    19561 tgacgacgtg cggcaacaaa acggcaaaat atttcccgac accctcgcca agctgtccgg
    19621 cgacatcgag gcgcacctga aaatcggaaa agtccaactt cccggcctgc aactggacga
    19681 tatggaaacc tacctccacg ccgacaaaga ccatatcgcg ctcagccgtt tcaagtcagg
    19741 gctttacggc ggccataccg aaggcggcat cagcatcgcc aacacccgtc ccgccactta
    19801 ccgcctgcaa cagaatgcaa gcaacatcca aatccaaccg ctgctgcaag acctgttcgg
    19861 cttccacagc ttcagcggca acggcgacgc ggtcatcgac ctgaccgcgg gcggcgaaac
    19921 ccgaaaagag cttatccgct cgcttcaggg cagcctgtcg ctaaatattt ccaacggcgc
    19981 gtggcacggt atcgacatgg acaatatcct gaaaaacggc atttcgggca aaactgccga
    20041 caatgccgca cccagcacac ccttccaccg attcacgctc aacagcgaaa tttcagacgg
    20101 catcagccgc cacatcgata ccgaactctt ctccgacagc ctctatgtta ccagcaacgg
    20161 ctataccaat ctggatacgc aggaattgtc tgaagatgtc cttatccgca acgccgtcca
    20221 tccgaaaaac aaaccgattc ccctgaaaat caccggtacg gtggacaagc cgtccattac
    20281 cgtcgattac ggcaggctga ccggcggcat caattcgcgc aaagagaaac agaaaatcct
    20341 cgaagacacc ctgctggaac aatggcagtg gctcaaacct aaagaaccgt aaacatcctg
    20401 cgtacaaaaa tgccgtctga aacgcccccg cgcttcagac ggcagaccgt aaaacctaca
    20461 accccaattc cccccaaatc ccatcaatct tagccgtaac cgcagggtct tttttgatga
    20521 cgcgtcccca ttcgcggtcg gtttctcccg gccatttgtt ggtcgcatcc aaacccattt
    20581 tgccgccgag tccgctgacg gggctggcga agtcgagata atcgatgggc gtgttttcca
    20641 ccaacacggt atcgcgtact gggtccatgc gcgtggttac cgcccagatg acttccttcc
    20701 agtcgcgcac gtttacatcg tcatcgacca cgatgatgaa tttggtgtac ataaattggc
    20761 gcaggaacga ccagcagccc atcatcacgc gcttggcgtg tccggcgtac tgttttttca
    20821 tgctcaccac cgccatgcgg taggagcagc cttcgggcgg caggtagaaa tcggtgattt
    20881 cggggaactg cttttgtaaa agcggtacga acacttcgtt caacgccacg cccaaaacag
    20941 cgggttcatc gggcggtttg cctgtgtagg tggaatggta aatcgggttt tcgcgcatag
    21001 tgatgcgttc gaccgtgaac acgggaaaat gatcctgctc gttgtaatag ccggtgtggt
    21061 cgccgtacgg gccttccaac gcggtttcgt tcggatggat gacgccttcc aacacgattt
    21121 ctgcgcgggc aggcacttgc aaatcgttgc cgatacattt caccagctcc gtccgcgaac
    21181 cgcgcagcag tccggcaaac tggtattcgc tcaaagtatc gggaacaggc gttaccgcgc
    21241 ccaaaatggt ggcggggtcg caaccgagca cgacagcgac gggatacggc gtatcgggat
    21301 tgagtttgcg gaattcctga taatccaacg cgccgccgcg atgcgacagc cagcgcataa
    21361 tcagcttgtt tttgccgatt agttgttggc ggtaaatgcc gagattttgg cgttttttgt
    21421 gcggcccgcg cgtgacggtc aagccccatg ttaccagcgg agcaacgtct tccggccagc
    21481 aatgctgaat cggaagttta tacaaatcaa cgttttcgcc ttcccacacg atttcctgac
    21541 acggcgcgtt tttcaccacg ttcggcgcca tgctccaaat gtctttcagt agcggcagtt
    21601 tggaaaacgc atctttgatg cctttgggcg gttcgggttc tttcaaatac gccagcgtct
    21661 gcccgatttc acgcagcttg gacacgctgt ccgcgcccat gcccatcgcc acacgttcgg
    21721 gcgtgccgaa caggtttgcc aacacaggat aaccgtagcg cgtaccgtcg ggcttaaccg
    21781 ggttttcaaa cagcaacgcc ggcccttcgg cgcgcagcac gcggtcggcg atttcggtca
    21841 tttccaaatg cggggaaacg gggtgcgcga tgcgtttgag tttgccctgc tgctcgagca
    21901 tggcgatgaa gtcgcgcagg tctttgtatt tcatattcat cctttttgtc cttttatcct
    21961 gagcaatccg attcggatac cgcccctatc cttgcctgcg cttcggcata ttctatgccg
    22021 tgataaaagt cgcgtaccag cggatgttcg ctgccttgat ggagttgcaa caaaggacgt
    22081 tgaccatcgg gttgggtaac gacattgcaa tgcaaaccga aggtgtcggt ttcataaggg
    22141 ggcagccggt tgcagatcat gccgaaataa acagcgtttt ccggattgtc gtgaaagtgg
    22201 ctttgatagt cgttaaaact cttttcgctg acggacaccc acacgccata ttccagcgtt
    22261 tcctcatgcc cgataatcgg aatcggcagc accgcgcgga taaagcggtc ggtttggtcg
    22321 ggatagcgga tgatgcagaa atcagaatcg cattctgctt gataagcaat gcgttcttct
    22381 tcactgagtt gattataggg atcgggggcg gtaaagccga ttgcgggcat atcttcatgt
    22441 ttttcgccgc aggaagtgca agtgtacata aggtttcaga ctttcaaaac gagtttgcgg
    22501 taaagccatt cgccggcaaa cagcatcccc atcaatacat aggcaatcac gcctgtataa
    22561 accgcccacc aatcatatcg ccccaaccgg gccaacaaag cggcaagcgt cccgttgatt
    22621 ataaaaaata cgcaccaaac ctgcgttacc cggcgggtat agcgcacggc tttttcaggc
    22681 aggtcgggct gttgcagccg cgcaagtttt tcaatcaccg tctgcccggc aaacaggctg
    22741 ccgccgaaca ccgccaacat catcagattg acgaggacgg gataccaata catcgaatca
    22801 tgccgcccga acaccaatac tgcggcaaaa aacagtgtaa taaacaaagc cgcataacgc
    22861 tgttggggca gtttggcagt cagggcgcgc agcagccaca acccgcacat cgccgccgcc
    22921 agccaaacaa accagcccgc ctccctgccg tatatagtgg attaacaaaa accagtacag
    22981 cgttgcctcg ccttgccgta ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
    23041 ccactatacc acaaagcggg ataggcaatg cttaatacgg tcagaaaaat atgtccgaaa
    23101 aaaccgggtt tcattttgaa tccgcacaaa tgttttcaga cggcatccga taaaaacatg
    23161 ccgtctgaaa aataattaga aatacccgat tagcccgcct gaatcttcaa taccgcctgt
    23221 accacgtcgt tgacggtgcg gacattgcgg aaatcttcgg cctgcagctt gcggccggtt
    23281 tcgcgcttga tgcggtcaat caggtcgatg gcatcgatgc tgtcgatttc caaatcttcg
    23341 taaagattgg tatcaggcgt aatccgttcc ggttcgattt caaacaactc ggtcagggta
    23401 tcgcgcaaca accggtagat ttcttgttcg gtcatatgtt tcatccttat gcttggcggc
    23461 ttttgacaaa ggcggcgagt gttttgacat tggcaaaatg ttcgcgcaag ttctcctgct
    23521 cgccgtccaa tcggaaaccg aaatgttttt gcaccgccaa gcccaattcc agcgcatcga
    23581 cggaatccag tcccagccct ccgtcgccga acagcgcgtc ttcgctgccg atgtcggcgg
    23641 cggttatatc ctccaaagcc aaactgtcga taatcagttg tttgatttgg ttttccaagt
    23701 cgttcatatg gttttccttg taaaatagtc ctgcagatat tcattcagcc gccgcgccgc
    23761 aatcggcaac ggtttctcgg cgagccagtc ttgggggagg atgtcgtctc cgacggtaat
    23821 ttcataccgt atccttttcg gggggatgcg gtaccacggc tgcccctttt taaaattggg
    23881 cggattcatt ttgatacata cgggcgtaat cacttcggca taccgcagtc ccaaagaaac
    23941 cgcgccccgg tgcattttta cccgtccgtc ccaccccgtc ctcgttcctt cggggaacac
    24001 cagcaggctc tgcccgctgt caaaaaccgc ctttaccgtt tccagcattg cttccgactc
    24061 ttcgttcgga atatagcccg cacctttaat ctggctgctc attgccggat tgtgctgcaa
    24121 atcttttttc acgatacagt tcattttggg cacatggccg acaagcagca ccacatccag
    24181 caaagacgga tggtttgcca aaatcaactg tcccgggcgg ttgagttttt caacaccctt
    24241 gaacgatacc tccaacacgc ccgaccattt cagataagca acgaacaaac gccaagatgt
    24301 tccgataatc cggcgcgccg ccaattggcg ggcgacagac cctgaagtgc cgttcaaagt
    24361 ataaggcaac aaaaccaatt tcatcataat gccgccgaca ccgaaaatca caaatcctaa
    24421 ccaagtcgca aaaaaacggc ggcaataatc caatttatcc attccgctgc cacagccatt
    24481 cacgattgcg atatacccgg cggcattctc gactgccgtt cagcagaaag cgcacccatt
    24541 ccaaaccgct ccaatatgcc tcgggcagca taccggcttc agacggcata tcgtccgaag
    24601 cagacaaagt caggctgtaa cgcgtccctt tggtcagaac catcgccaaa gcataagcaa
    24661 acggcgcgcg agtcgccgat acggcatatc cttccggcag cggatcgtcc gccgccaaaa
    24721 ccaaaaccga cccgcatccc tcttccaaca gtgatgccgc ttccgccaat gccgtttcca
    24781 caccgtcggc acacacggcc aatgccgtct gctcgctcat atcctgacgc aatatcgacc
    24841 attgccccgc cgttgcattg tgcaccgaca aaccgaacga agtcggcgac acggtatgcg
    24901 atttcaacag ttccaaccac aaatcgaaac tgcgtgccat ttccccgtcg tgcgaggcat
    24961 aaactaccgg actgccggga tgggcggagg caatgtccca agccgcgtcg cataccaaac
    25021 gcgccgcctt actcaaacgg cggcgctgca tagcgggcag gaacggcaat tccggcctga
    25081 catcgggcaa accgtcggca aaatccggac attccgccca ttttgcccac tgggccatat
    25141 cgcgcatttt gctgcccgaa acccgccagg cggcgatgtc gaagtggaac cggcaaataa
    25201 tcgacggcat agtttctttc aaaaatttac actgtgccgc attctaacca aagcctatcc
    25261 ccctgacaat gccgaaattc aaacgcattt ctgccccctt tctccgacaa cgccgcccct
    25321 cggaaaaccg ccagaattag cctgaattta catttatcat tataatgccc gtatttgcca
    25381 gcctgccgcc gcaatatatg gacacactgc tagaatgccc gattaccaac accgcctccc
    25441 tgctgccgca cagcgggcgt atggttctga tagaccgcat tacccgatac ggcgatgatt
    25501 ttgtcgaagc aggggcacag gtaagcccca atcacatcct tttacttgac gacaaactgc
    25561 cctacacggc atttatcgaa ctgatggcac aggctgtcgg cgcgtatgcc ggtatccaag
    25621 cccgaaaaaa cgcacggtcg gtccggctcg gcttcctgct cggcacgcgc aaacttgaaa
    25681 tcttcgccca atccgtccca atcggcacgc atctgctggc aacggcgcat atgtctattc
    25741 aggatgccgg gggtatgggc gtgtttgact gcgaactgcg ttggacagac gcgccggaaa
    25801 cttcgtccga aacactccct tcagacggca ttttggcgcg cgcctcactc aacgtgtaca
    25861 gccccgaaca ccctgccgga acaaccgatg ccgtctgaac aggcacacac atacggagaa
    25921 caacgatgac cgaaactgtc ctgattaccg gctccaacag gggcataggc aaagccgtcg
    25981 cattcggttt ggcggaagac ggctttgata tcgctgtcca ctgccgcagt cgccgcgacg
    26041 aagccgaagc cgtggcggaa gaaatccgcg ctttgggcag aaatgcgcgc gtgttgcagt
    26101 ttgacgtgtc cgaccgcgaa gcctgccgcg agattctgac cgccgacatc gaagcaaacg
    26161 gcgcgtatta cggcgtggtg ttgaacgccg gactgacgcg cgacaatacc ttccccgcgt
    26221 tttcagatga cgattgggat gtggtgctgc ggactaattt ggacggtttt tacaatgtat
    26281 tgcatccgct ggttatgccg atgatacgcc gccgcaaagc cggacggatt gtgtgtatgg
    26341 catcagtgtc cggcctgacg ggcaaccgcg ggcaggtcaa ttacagcgcg tcaaaagcag
    26401 gcattatcgg cgcggcaaaa gccttggcgg tcgaactggc gaaacgcaaa atcaccgtca
    26461 actgtgtcgc gccgggtctc atcgataccg atattatcga tgagaacgta cctgtcgaag
    26521 aaatcttaaa ggctgtcccc gcagcgctta tggggctgcc ggaagaagtg gcgcacgcgg
    26581 tgcgtttcct gatggatgaa aaagcggcgt acatcacgcg ccaggtgatt gcggtgaacg
    26641 gaggtttgtg ttgaatacca gaagggtcgc agtaacaggc ataggcggca ttaccgcctt
    26701 cggccgggat tggcaaagca tacaggcagc attcaaagcc gaaaaaaacg ccgtcaaata
    26761 tatggattgg cacgaacgtt tccccgaatt ggaagcgcaa ctgggtgcgc cgattgaaaa
    26821 ttacgcgccg ccgaaacatt ggacgcgcaa gcagctcaga agtatggggc gcgtgtcgta
    26881 cctgtgcgtc gatgcggcgg agcaggcgct ggcggatgcc ggtttgctcg gggacgaaag
    26941 cattaccgac ggacggatgg gcgttgcctg cggctcttcc agcggcagca ccaaagacat
    27001 cggcgatgtg ggcgaattgt tgctgaccgg cacgtcgcgc aacttcagcg ccaacaccta
    27061 tgtgcgtatg atgccgcaca ccaccgccgc caatatcggc atctttttcg ggctgaaagg
    27121 gcgcatcatc ccgacatcga gcgcgtgttc gtccggcagc caaggcatag gttatgccta
    27181 cgaagccatc aaatacggtc tgaccgatat gatgctggcg ggcggaggcg aagaattttt
    27241 cccgtccgaa gtgtatgttt tcgactcgct ttatgccgcc agccgccgca acggcgaacc
    27301 ggaaaaaacc ccgcgcccat acgacgcgaa ccgcgacggg ctggtcatcg gcgaaggcgc
    27361 ggggattttc gtgctggaag aattggaaca cgccaaacgg cgcggtgcga taatttacgc
    27421 cgaactcgtc ggctacggag ccaacagcga tgcctaccat atttccacgc cccgccccga
    27481 cgcgcaaggc gcaatccttg cctttcagac ggcattgcaa cacgcaaacc ttgcacccga
    27541 agacatcggc tggattaatc tgcacggcac cgggacgcac cacaacgaca atatggaaag
    27601 ccgcgccgtt gcagcggttt tcggcaacaa tacgccctgc acgtccacca agccgcaaac
    27661 cggacacacg ctgggcgcgg cggacgcaat cgaagccgcg ttcgcgtggg gcattgccga
    27721 ccggcaaagc aatcccgaag gaaaacttcc gccccggctt tgggacgggc agaacgaccc
    27781 caacctgccc gccatcaacc tgaccggcag cggcagccgc tgggaaaccg aaaaacgcat
    27841 taccgccagc tcgtcgtttg ccttcggagg aagcaactgc gtcttaatca tcggatgaaa
    27901 taagtttgtc aatcccaccg ctatgctata caatacgcgc ctactcttga cgggtctgta
    27961 gctcaggggt tagagcaggg gactcataat cccttggtcg tgggttcgaa ccccaccgga
    28021 cccaccaatt cccaagcccg gacgtatgtt tgggcttttt tgccgccctg tgaaaccaaa
    28081 atgctttgag aaaccttgat aatgaaaaaa gtcagcgtat tgattgttgc caaaaacgaa
    28141 gcaaaccaca ttcaggaatg tattgaaagt tgccgtttcg ataaagaagt catcgttatc
    28201 gacgactaca gcaccgacaa tactgccgaa attgccgagg gtttgggcgc aaaagtcttc
    28261 agacggcatt tgaatgggga tttcggagcg caaaaaacat ttgccatcga acaggcaggc
    28321 ggagaatggg ttttcctgat tgatgcagac gaacgctgca cgccggaact atctgatgaa
    28381 atctcaaaaa ttgtccaaac cggcgattat gccgcctatt ttgtcgaacg ccgcaacctt
    28441 ttccccaacc atcccgccac acacggcgcg atgcgtcccg acagcgtatg ccgtctgatg
    28501 ccgaaaaaag acagttcggt gcaaggcaaa gtacacgaaa ccgtacaacc cccctacccc
    28561 aaacgccgtc tgaagcattt tatgtaccat tacacgtacg acaactggga acaatatttc
    28621 aacaagttca acaaatatac ttccatttca gccgaaaaat accgagagca gggaaagccc
    28681 gtgcgtttcg ttagggacat tatcctccgc ccgatttggg ggtttttcaa aatttatatc
    28741 ctgaacaaag ggtttcttga tggaaaaatg ggttggatta tgtccgtcaa ccacagctat
    28801 tacacgatga ttaaatatgt caaactatat tatctgtaca aatccggcgg aaaattttaa
    28861 atggaaaaag aattcaggat attaaatatc gtatcggcca agatttgggg tggaggcgaa
    28921 caatatgtct atgatgtttc aaaagcattg gggcttcggg actgcacaat gtttaccgcc
    28981 gtcaataaaa ataatgaatt gatgcacagg cgattttccg aagtttcttc cgttttcaca
    29041 acgcgccttc acacgctcaa cgggctgttt tcgctctgcg cacttacccg ctttatccgg
    29101 aaaaaccgca tttcccacct gatgatacac accggcaaaa ttgccgcctt atccatactt
    29161 ttgaaaaaac tgaccggggt gcgcctgata tttgtcaaac ataatgttgt cgccaacaaa
    29221 actgattttt atcaccgcct gatacagaaa aacaccgacc gctttatttg cgtttcccgt
    29281 ctggtttacg atgtgcaaac cgccgacaat ccctttaaag aaaaataccg gattgttcat
    29341 aacggtatcg ataccggccg tttccctccc tctcaagaaa aacccgacag ccgttttttt
    29401 accgtcgcct acgccggcag gatcagtccg gaaaaaggat tggaaaacct gattgaagcc
    29461 tgtgtgatac tgcatcggaa atatcctcaa atcagactca aattggcagg gcacggacat
    29521 ccggattata tgtgccgcct gaagcgggac gtatctgctt caggagcaga accatttgtt
    29581 tcttttgaag ggtttaccga aaaacttgct tcgttttacc gccaaagcga tgtcgtggtt
    29641 ttgcccagcc tcgtcccgga ggcattcggt ttgtcattat gcgaggcgat gtactgccga
    29701 acggcagtga tttccaatac tttgggcgcg caaaaggaaa ttatcgaaca tcatcaatcg
    29761 gggattctgc tggacaggct gacacctgaa tctttggcgg atgaaatcga acgcctcgtc
    29821 ttaaaccccg aagcaaaaaa cgcactggca acggcaggac atcaatgcgt cgccgcccgt
    29881 tttaccatca accataccgc cgacaaatta ttggatgcaa tataagccgc tttcagacgg
    29941 cctatgccgt ctgaaagcct ttgatgcaac aaaccgccaa attatattcg ttcattggaa
    30001 agaaacaccc cgaatccatc cttcaaaata agaaaatccc aatatctccc aatattacgc
    30061 agcctattgg caaagttttg cagcgtcttc cccggcttgt gctgccgcat caagtgcttt
    30121 gttacaatgt atagtggact aacaaaaacc agtacagcgt tgcctcgcct tagctcaaag
    30181 agaacgattc tctaaggtgc tcaagcacca
//
LOCUS       DAWHRS010000007        25831 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWHRS010000007 DAWHRS010000000
VERSION     DAWHRS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.007"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WIB82_000226"
     CDS             complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WIB82_000226"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ3841596.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAPAAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKNGVVSRLTGGLAGMAK
                     GRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMINTKTVAVEPKEDGVYVTFEGTNAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVVVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELAAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1968..3575)
                     /gene="aceF"
                     /locus_tag="WIB82_000227"
     CDS             complement(1968..3575)
                     /gene="aceF"
                     /locus_tag="WIB82_000227"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ3841597.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPAA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGDDIKAFVKSVMQ
                     GGAAKPAAASASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPIWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3725..6388)
                     /gene="aceE"
                     /locus_tag="WIB82_000228"
     CDS             complement(3725..6388)
                     /gene="aceE"
                     /locus_tag="WIB82_000228"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ3841598.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDTQLKSSGDREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVVT
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7082..8794
                     /locus_tag="WIB82_000229"
     CDS             7082..8794
                     /locus_tag="WIB82_000229"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ3841599.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNG"
     gene            8857..9513
                     /locus_tag="WIB82_000230"
     CDS             8857..9513
                     /locus_tag="WIB82_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ3841600.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9523..9978)
                     /gene="ruvX"
                     /locus_tag="WIB82_000231"
     CDS             complement(9523..9978)
                     /gene="ruvX"
                     /locus_tag="WIB82_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ3841601.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9971..10519)
                     /locus_tag="WIB82_000232"
     CDS             complement(9971..10519)
                     /locus_tag="WIB82_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ3841602.1"
                     /translation="MNLSNHFLIAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10521..11081)
                     /locus_tag="WIB82_000233"
     CDS             complement(10521..11081)
                     /locus_tag="WIB82_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ3841603.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENLDKR"
     gene            11409..13214
                     /locus_tag="WIB82_000234"
     CDS             11409..13214
                     /locus_tag="WIB82_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ3841604.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13334..14818
                     /locus_tag="WIB82_000235"
     CDS             13334..14818
                     /locus_tag="WIB82_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ3841605.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLNGAVGVSAAFLPPDALVVSTKGREG
                     TEGFALKARPEDYIFKAGTDPLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15147..17180
                     /gene="uvrB"
                     /locus_tag="WIB82_000236"
     CDS             15147..17180
                     /gene="uvrB"
                     /locus_tag="WIB82_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ3841606.1"
                     /translation="MEVIQYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRNDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRTVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQMKFNEEHGIVPQQIKKQVKDIIDGVYHEEDGGKGRLKGKGKNKV
                     KVGEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKENLLFNAG"
     gene            17200..18309
                     /locus_tag="WIB82_000237"
     CDS             17200..18309
                     /locus_tag="WIB82_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841607.1"
                     /translation="MKLFDEKKRYEKSEIRKNRSLYDFFDESSLEKYEKIRNLLNEAL
                     SLYDEASQNRIIDDIVKNKNSDEKFYSNCFELIVSYMFRKIGQYELEPHPILANNKKP
                     DFLVTVPNFGKFYLELVTVDENDMGDLQFNILKTYIKKFRNTNELSNEVTVISIKSLN
                     GRNFSHENVMDLQNAIRVWWGENKNNIGSKLEKEMNGNLIHFEIVKENSLRVESIIDE
                     TDFYKKFLSSLEYKSKKYGELDLPYSIAITFRPSNLTDGLILQENFIASCLYGGFYYE
                     PKSVKKRKIHSLWEVFEENKRNNQVSGVLYFDSLIPENLESFRYSLFLNYYSKFELPH
                     DLKSFLPFYCVVNKSEAFQSEIIEKGDVMASGRFI"
     gene            18456..18946
                     /locus_tag="WIB82_000238"
                     /pseudo
     CDS             18456..18946
                     /locus_tag="WIB82_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            18933..19178
                     /locus_tag="WIB82_000239"
     CDS             18933..19178
                     /locus_tag="WIB82_000239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841608.1"
                     /translation="MNTIKAMSDSGADLRKTKDFIRATACWLMRWLNLKNSTSVHNTQ
                     GIRTKEAVEQGLRGNNRPEKGIGNRWNYDGFIKELAK"
     gene            19228..19512
                     /locus_tag="WIB82_000240"
     CDS             19228..19512
                     /locus_tag="WIB82_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841609.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWARGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(19637..20353)
                     /gene="trmB"
                     /locus_tag="WIB82_000241"
     CDS             complement(19637..20353)
                     /gene="trmB"
                     /locus_tag="WIB82_000241"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ3841610.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPVDLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(20475..21878)
                     /locus_tag="WIB82_000242"
     CDS             complement(20475..21878)
                     /locus_tag="WIB82_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ3841611.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EERLPEAQVRLMYLLYASRHFEEALEWAKTSTKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDKAVYRFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLTAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAQKDSMALYNLACIHYNGHGVEPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            complement(21996..23882)
                     /gene="uvrC"
                     /locus_tag="WIB82_000243"
     CDS             complement(21996..23882)
                     /gene="uvrC"
                     /locus_tag="WIB82_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ3841612.1"
                     /translation="MPSETVFQVIDMNKETRFPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPHSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEQVEGISKALAETIY
                     NHLH"
     gene            complement(23946..>25831)
                     /locus_tag="WIB82_000244"
     CDS             complement(23946..>25831)
                     /locus_tag="WIB82_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ3841613.1"
                     /translation="TINVPFLIGMAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYK
                     SAWASIKGGLANMDVLVTIGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGVMVIGFVS
                     LGKFLEHRTKKSSLNSLGLLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERI
                     AADGIIESGSGWADESHLTGESNPEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLG
                     DMMNALSEAQGSKAPIARVADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVA
                     VLVIACPCALGLATPAAIMVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEG
                     SPQVAAVYCVPDSGFDEDALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQT
                     VVGAGITAEVEGVGLVKAGKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALAD
                     ALKADTAEAIGRLKKHNIDVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQK
                     LKAAGKTVAMVGDGINDAPALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALL
                     VSQATLKNIKQNLFFAFFYNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRV
                     KID"
BASE COUNT         6158 a         6262 c         7117 g         6294 t
ORIGIN      
        1 ccggaggaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggaaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggccaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgccag
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcagca gcaacgtggc
      541 cttcgtgaac ggctttgtgt gccaacatcg gttgaccgac gatgtcaccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacga
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcacggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttcg gtgcgttcgt gccttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gatcatgatg ttgtcaaaac
      841 gatattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaacgacg tcaaggcgtg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagtgcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggtaacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc caagtggtgc ggatctaaga
     1201 attgcccgtc gccttggata acatccactt tacggccttt cgccatacct gccaaaccgc
     1261 cggtgaggcg ggaaaccacg ccgtttttgt aggcgcgcag catatcgatg tcgagttcag
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacaa ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgttc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccggg accgccaccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagca ggagcagcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 caggtgcttc agctttggga gcggctgccg tgccttcagc ttcaacgacg acaatcaagc
     1681 cgccttcgga gattttgtcg ccgactttga ctttcacttc tttgaccacg cccgcaactt
     1741 cggcaggcac gtccatcgtc gctttgtcgg tttccagggt aatcagggta tcgtcaacgg
     1801 cgatggtgtc tcctacgttc acttcaactg cgataatatc tacattttcg tgtccgccaa
     1861 tgtcgggcac tttcaattca actaagctca tgtctcatct ttctgaatga tgttcaaact
     1921 tccttttcag acggcacagg ccgctatgcc gtctgaagcc gatcgggtta cagggtaatg
     1981 cggcggaagt ctttcaacag cttcgccaag aatacggtaa agcgcatacc ggccgcaccg
     2041 tcgatgacgc ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg ggcaaactct
     2101 ttgccgttcc aaatcggttt gatttgggat ttgcacacgc ccaagatagc aacttcagga
     2161 gcattcacga ttggtgtgaa gcctgtgccg ccgataccgc ccaagctgga aatggtaaag
     2221 cacgcgcctt gcatttcttg cggtttgagc ttgccttcgc gggctttttt ggacaattcg
     2281 gtcagctctt ggctgatttg tttcaagcct ttttgatcca cgtctttgat gacgggaaca
     2341 accaagccgt tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt cagcaccagg
     2401 ttgtcgccgt ccagagaagc gttgaattcg gggaaggctt tcagcgcgga aacggaggct
     2461 ttgatgatga acgccaacgg tgacagtttc acgccttcgc gttcccattc tttgttcagt
     2521 tgtttgcgga attcttccag ctcggtcata tctgcttctt cgtgtacggt aacgtgggga
     2581 atcaccaccc agttgcgaga caggttttga ccggagattt tcttaatgcg ggacaattct
     2641 ttaacttcga cattgccgaa tttggagaag tccactttag gccacggcag taagtccaga
     2701 ccgccgccca aagatgcgct ggctgcggca ggttttgccg cgccgccctg catcacggat
     2761 ttcacaaagg ctttgatgtc gtcgcccatg atacggcctt tcaagccggt gcctttgact
     2821 tggcccaaat ccacgcccaa ttcgcgcgcc agtttgcgtg cggaaggacc ggcgtgtgct
     2881 ttggcgaaag cggcctcgtc gattttggca gcggcaggtg cagaaggtgc aggcgcggcg
     2941 gcgggtgcgg cggcagcagt cggaggcgga gcggcagccg gtgcggcagc ttgagcggct
     3001 tgagcagggg ctgctgcggc agagccgacg gtttctactt cgataatggc agagccttcg
     3061 gatactttgt cgccgacttt taagaatacg gctttaacga caccggcagc ggtacaaggt
     3121 acgtccattg tcgctttatc ggtttccaaa gtaatcagcg tgtcgtcttc ggcaacggtg
     3181 tcgcccactt tgatttcaac ggcgattaca tccacatcgg tatggccgcc gatatcggga
     3241 acggctactt gaacggttgc accgcctgcg gcagcgggtg cggcagcagg tgcaggttgt
     3301 gcttcggcag cagccggggc ggcttcggcg gcggcggcac cggtttcaac ggtcagaatt
     3361 acgccgcctt cggagatttt gtcgccgact ttgactttca cttctttcac gacaccggcc
     3421 gcatcggcag gcacatccat cgtggctttg tcggtttcca gtgtaatcag ggtgtcgtca
     3481 acggcgatgg tgtcgcccgc tttaacttct acggcgatga tgtcgacgtt ttcgtgaccg
     3541 ccgatatcgg ggactttgat ttctacgata ctcatttgag ttcctttaat cttgttcaat
     3601 tggatgccgt ctgaaagctg cttcaggcgg catcggtacg gtttgtctgc tcaaaccatt
     3661 cgggcattcg gttttcagac ggcctgatgt cggaatgggg caaacagacc gtctgaaaca
     3721 tcaatcagcg tttccagcta ggagctgaat cggctttgat gccgtatttc tcaatggctt
     3781 gttgaacggt ttctttgctg actttgcctt gttccgccaa tgcgctcaat gctgccacgg
     3841 caacgttgta gcgatccact tcaaagaagc ggcgcaggtt ggcgcggctg tcggagcggc
     3901 cgaagccgtc agtacccaag acgtggtagt cgttcgggat gtaggcgcgg atacggtcag
     3961 catagctgcg gatatagtcg gtagcggcaa taaccggacc gtcatgacct tgcaatttgg
     4021 aagtaacgaa aggtactttt tcggcttcca gcggatgcag gcggttgaag cgttctacct
     4081 cgacagcgtc gcggtgcagc aggttgaagg atgggcaaga ccaaatgtct gcttccacgc
     4141 cgaagtcggc tttcagcagc tcggcaccgg taaccacttc ttgcaaaatc gtaccggaac
     4201 ccatcagttg gactttctta tcgcctttgc cgccggcttt cagcaggtac atacctttca
     4261 agatgtcttg ttccgcacct tcgggcatat ccggatgggt gtagttctcg ttcatcaggg
     4321 tgatgtagta gaacacgtct tcattattgg catacatacg gcgcagaccg tcttgtacga
     4381 tgacggcgac ttcgtattgg aaagtcgggt cataagatac gcagttcgga atcaggtcgg
     4441 cctggatgtg gctgtggccg tcttcgtgtt gcagaccttc gccgttcagc gtcgtacggc
     4501 cggcagtacc acccaacagg aagccgcgtg cgtgcatatc acctgccgcc caagccaagt
     4561 cgccgatacg ttggaaaccg aacatagagt agtagatgta gaaaggaatc atcgcaaagt
     4621 tgctgttggc gtagctggtt gcagcagcga tccagtcagc cattgcgccc ggttcgttaa
     4681 taccttcttg caagatttga ccgtcaacgg attctttata gaacatcagt tggtctttgt
     4741 cttgaggggt atattgctga cctttcggat tccaaatacc gtattggcgg aacatgcctt
     4801 ccataccgaa agtacggctt tcgtcgggaa cgataggtac gacgcgtttg ccgatttttt
     4861 tgtctttcaa caaggttgac aaaatgcgga caaatgccat agtggttgag aattcgcggt
     4921 cgccgctgga tttcagttgg gtatcaaatg cggacagctc cggcacttcc aaaacttctt
     4981 gggtaggttt gcgttgcggc aggtagccgc ccaaagcatc gcggcgtgcg tgcaggtatt
     5041 tgtattcttc cgtatcgggg gcaaaagtca ggtaaggcag atcgccgctt tcgatttgtt
     5101 cgtcggtaac cggaatgtca aagcggtcgc ggaattgttt cagggacgct ttgtccattt
     5161 ttttggcttg gtgggcaacg ttctgacctt cgccggatgc gcccataccg taacctttaa
     5221 tggttttcgc caagatgacg gtaggtttgc cgtcggcatg gttcgctgcg cggtcgtagg
     5281 cgttgtacac tttttgcggg tcgtggccgc cgcggttcaa tgcccagagt tgctcatcgg
     5341 tcatatcggc aaccaatgct ttcagttcgg gcgtattgaa gaagtgttcg cgcacatacg
     5401 cgccgtcttt ggatttgtaa gtttggtagt cgccgtccaa acattcttcc atacgttggc
     5461 gcaggatacc gtctttgtct ttcgccaaga ggcggtccca gcggcggccc caaatgactt
     5521 tgacgacatt ccagccggcg ccggcaaagt tgccttccaa ttcttggatg attttgccgt
     5581 tgccgcgtac cggaccgtcc aagcgttgca gattgcagtt gatgacgaaa atcaggttgt
     5641 ccaagccttc gcgtgcagcc agtgcgattg caccttgaga ttcgggttcg tccatttcgc
     5701 cgtcgccgca gaaacaccat actttacggc ctttggtttt tgccaaacca cgcgattcca
     5761 agtatttcag gaaacgcgcc tgataaatcg ccatgatggg ccccaagccc atggatacgg
     5821 tcgggaactg ccaaaagtcg ggcaagaggt gggggtgcgg ataggaaggc agaccgtgtc
     5881 cgtccacttc ttggcggaag ttgttcagct ggtcttcggt cagacggccc tcgacgaatg
     5941 cgcgtgcata gatgcccggg gcgacgtgac cttggaagaa gaccaaatcg ccttcttcgc
     6001 cttcgccttt ggctttccaa aagtggttga aaccgacttc gtacatggtg gcggcagatt
     6061 ggaaagatgc gatgtgccca cccagttcca aatctttctt gccggcgcgc aatacgatgg
     6121 cggcggcgtt ccagcgcacg aatgcgcgga tgcggtgttc gatgttttgg tcgcccggaa
     6181 tgcctttttc gttttcaacc gaaacggtat tcaaatacgg ggtggtcgtg ccgtgtggca
     6241 tacgtacgcc cttgtcgcgg cagtatttga ccaggttttc caagaggtat tgtgcgcgtt
     6301 cgccgccttc atattcgagg acggagctta acgcgtccag ccactcttgg gtttcgatag
     6361 ggtcaacatc gtgtaattgg gtggacatag tatctatcct ttatgttgag tgtttaatcg
     6421 gcagggaggg tgtagccaac ccctttattt tcgtttgtga aaaataataa gcaaaaacga
     6481 aaatttctaa atcaaaccga aaaaaataat ttaagtttaa tgttttcaaa aaattataaa
     6541 agttttggaa acaagggaaa acttgaaaaa aatgttcgat ctgctccaca cgaaatccta
     6601 tcagcaatcg ggtctgctgg caaataacca tgcctgtaaa agcaggtttt atactgaagt
     6661 tggcgtttgg taaggtcaag tctttttgat ttcgaaatat tctgtttgat tttggcgata
     6721 ttttttcgga attgtggtat atcgaatcat tatgtctctg tcggcaattt tcctattgta
     6781 gaaaatctat acacaagctt tgtggataac cgtgttgata agccacggct tgtgttaaaa
     6841 aagtcttacg caacaaatgt acgcttattt tgattaaaaa ataggcacat ttaaatctat
     6901 aaaaatcatg tgcttatgtt caatatgatg tttctggtca tgtttttgcc gtctgttatg
     6961 ggatttgtcc cgcgcttggg gcagggggta aattttggag gttggtgcaa aacctgcttt
     7021 tttcgcgtta caatctgcac ccgatttact tttgaatttg cctgtttgga aaaatacttt
     7081 tatgaaagcc agccaattct ttatctctac tttaaaagaa gcccctgccg aagccgcgct
     7141 tgccagccac aagctgatga ttcgcgccgg tctgattaaa gccaacgcgt ccggccttta
     7201 tacttggatg ccgatggggc tgcgcgtgtt acgcaaagtc gaaaacgtcg tgcgcgagga
     7261 aatggcgcgt gcgggcagcg tggagttgct gatgccggtg gtgcagcctg ccgagctgtg
     7321 gcaggaatcc ggccgctggg agttttacgg taaagagttg ctgcgcctga aagaccgcca
     7381 cgaccgcgat ttctgcatgg gtccgacctg tgaagaagtc atcgccgaca tcgtgcgcaa
     7441 agaaatcaac agctacaaac agctgccgaa aaacttttac cacatccaaa ccaaattccg
     7501 cgacgaagtg cgcccgcgtt tcggcgtgat gcgcgcgcgc gagttcgtca tgaaagacgc
     7561 ttattccttc catgccgact acgcctcgct tcagacgacc tatgatgcca tgtatgacgc
     7621 ttactgccgc atcttcaccc gtctgggctt ggcattccgc cccgtcgccg cagacaccgg
     7681 cagcatcggc ggtacgggtt cgcacgagtt tcaagtgttg gcggaaagcg gcgaagatgt
     7741 gattgcgtac agcgacactt ccgattacgc cgccaatatc gagttggcac cgaccttgcc
     7801 gcttaaaggc gaacgcaccg ctgctcaggc cgagttgacc aaagtacata caccaaacgt
     7861 caaaaccatt gagtctttgg ttgaattcct gaatattccg gttgaacaaa ccctcaaatc
     7921 catcgtggtt gaaggcgaaa acgaaggcga aatcgtttta ttgctgttgc gcggcgacca
     7981 tgagttcaac gacatcaaag ccgaaaaact cgcaggtgtg aaatcgccat tgacgatggc
     8041 aagccctgcc gcgattgtcg aacagttcgg cgcaaacggc ggctcgctgg gtccggtcgg
     8101 ctttgcaggt aaagtttatg ccgatttcgc cactgaaaaa ggcgcggact gggtcatcgg
     8161 tgcgaacgaa gacggctacc actacaccgg cttcaacttc gggcgcgatg ccgccgagcc
     8221 tgaattcgtc gatttgcgta atgtggtcga aggcgacgaa agccccgacg gacaaggccg
     8281 tctgaaactg gcgcgcggca ttgaagtcgg acacgtcttc cagttgcgcg acaaatatac
     8341 taaagccatg aacgtcagct tcctcgacaa caacggcaaa tcgcaaatta tggaaatggg
     8401 ctgctacggc atcggcatca cccgcgtcgt tgccgccgcc atcgagcaga ataacgacga
     8461 aaaaggcatc atctggacca aagcaatggc gccgtttgaa gtcgttatcg tgccgatgaa
     8521 ctacaaaaaa tcagacaccg tacgcgaagc cgccgacaaa atctatgccg aattgctggc
     8581 ggcaggcgcg gatgtgctgc tggatgaccg cgacgaacgc gcaggcgtat tgctgaatga
     8641 ctccgagctg ttgggtattc cgcaccgcat cgtcatcggc gaccgcgctt tgaaagaagg
     8701 caatgtcgaa tacgccgaac gccgcaacaa cgaagcgcag gcagttgcaa tcggagaaat
     8761 tgttgcgcgt gtaacagctt cattaaatgg gtaagcttgg tcaaatgccg tctgaaaata
     8821 atttcagacg gcatttttat ttggggagaa agtgaagtgg cctcagtatt tttagaagga
     8881 gaagcagtcg aggtcggcaa tattttctgt atcggcagga actatgccgc acatattgaa
     8941 gagttaaaaa acgaaatacc ttccgaacct gtggtgttta tgaagccgtc aggcagcatt
     9001 ttgaacagcg gcggtacaat cttactgccc gagttcagta gggatgtgca gtttgagtgc
     9061 gaactcgtgc tgctggtcgg taaggattca gacggcacgg gcgagggtaa agatattttg
     9121 ggatgtgtcg caggatatgg tgtagggctg gaccttacgg cacgggatat ccaatgccgt
     9181 ctgaaggaaa aagggctgcc ctggctgaag gcaaaagggt tcaggcattc cgcgtgcgtg
     9241 tcggattttg cggcggcagg cagaatcggc aatcccgaga atgtgttgtt ttcattaaaa
     9301 caaaacggcg tattgaaaca gcgcggggat accggtttga tgatttaccc gattcgggaa
     9361 attttacaca aactggcggc agattacggt ttggggaaag gggatttggt gtttaccgga
     9421 acgccctccg gcgtgggcgc aatcggtgcg ggggacaatt tggcattgga gctggacggc
     9481 ttggtgcgcg cctctttcac ggtagggtgt taaccgcgcc gcttaacctt cacgtccgtt
     9541 gaaacattcc gccggaccgc cctcgaaaaa accgtgcaag atggcttgcg ccgccacttg
     9601 gtcgagcacc gatttgcgtt ttttgccgaa gacctgtgct tccgaaagca ggctttcggc
     9661 atagacggac gacagccgtt cgtcaaccca atagacgggg agattgaacc tgccgttcag
     9721 cctgcgtccg aacttgcgcg acaggtgcgt catttcatgt ttcgtgccgt cggtatgcac
     9781 gggcagtccg acgacaaaat aacgcggctg ccattcttga accagcttgg cgattgccgc
     9841 gaacttttca tcgttgctgc cgccggtaac ggtgctcaaa ggatgggata gccctaattc
     9901 cgcgtctcct tgtgccacgc cgatacgcgc ttcgccgaag tcgaatgcca gtgccgttcc
     9961 ttttggaatt ttatgcatgg ccggctcccg aaaacagggc gagcgggtcg atgccgagtt
    10021 tggcgaatgc ggcggcgtaa cggtgttcgt aggggatgtc gaacaggatg tgttcgtcgg
    10081 cgggaacagt cagccacgca ttgtcggcaa gttcgcgttc gagctgccct ttgctccagc
    10141 ttgaatagcc tatgctgatc aaggctttgt caaccgcacc ttcgcgtgaa atattttcaa
    10201 tcacgtctcg ggaagaagtt agcgcgatat tgtctgaaac gccgatactg ctttgccagt
    10261 tgccgatcgg ggtatgcacg acataaccgc gctcgacctg caccggaccg cccatcatca
    10321 cgctgtcgtg ctgcatccgc atggggatgt ttttgccggt ggcggaaaaa atcatgtcca
    10381 tcgtaatcgg agagggtttg ttgatggcga tgccgagtgc gccgtcttca tcgtgtttgc
    10441 agatatagac gaccgattgt gaaaaaaacg cgtcttccat atcgggcatg gcgatgagaa
    10501 agtggttgga gaggttcata tcagcgtttg tcgagatttt ctatcgggtt ggaaatcatg
    10561 caggcgccgg caagcaggtt gccggaagta tcggcttgca ccccatcggt ttgcggtatg
    10621 ccgccgccga aacaggaaac cgcgcttaag gaatttttcg gcgagccttg gatgatttta
    10681 tcgctgtaca ccaaataggc gaaggttttg cgtttggggt cgtaataacg gacaatctgc
    10741 cggctcttga acgcgaagct cgcaccgtgt ttgaaaactt ctttcggttt gcgcacggcg
    10801 gtttcgtcaa aagaaatcga agatgccgtc tgaacgcacg aaaccgatgc gtcggacgcg
    10861 tcctcttcca aattgaccat ttctttcaag ccgccttttt ttgcatacga aatataacag
    10921 gcaacccctt gaacgtcggg atcgtcgaat ccttccactt cgatacggtc gtttttgccc
    10981 agcatgttga aaacggtact tgcccgtccg attttatcgg tttcgccgct gccgcaggca
    11041 gtcagcagga cggcggcaga cagcagtagc agtctgttca tcaaaatttc cttatcggtt
    11101 gaatccccgt tttccgtgaa ggcggcaggg cttgctttgt ttggaggggc gcacgttccc
    11161 aaaatcgggc aacatacggt atgcggctta atgtgctacc ctgtgtgtcg gaacacaata
    11221 tccgttgcgg cattgaaaat atgacgcaca atataaggct gtttgagccg attgcaagta
    11281 tccgcgcaaa gaaggcgcaa gattgggcaa acggcatttt cagacggcat aactgacagt
    11341 ataatccaaa cgtccggctg ctttcgtgca gccttcagta ttatccaact ttttccgaag
    11401 gtcgaaccat gcgctacaaa ccccttctgc ttgccctgat gctcgttttt tccacgcccg
    11461 ccgttgccgc ccacgacgcg gcacacaacc gttccgccga agtgaaaaaa cagacgaaga
    11521 acaaaaaaga acagcccgaa gcggcggaag gcaaaaaaga aaaaggcaaa aatggcgcag
    11581 tgaaagataa aaaaacaggc ggcaaagagg cggcaaaaga gggcaaagag tccaaaaaaa
    11641 ccgccaaaaa ccgcaaagaa gcagagaagg aggcgacatc caggcagtct gcgcgcaaag
    11701 gacgcgaagg ggataagaaa tcgaaggcgg aacacaaaaa ggcacatggc aagcccgtgt
    11761 ccggatccaa agaaaaaaac gcaaaaacac agcctgaaaa caaacaaggc aaaaaagagg
    11821 caaaaggaca gggcaatccg cgcaagggcg gcaaggcgga aaaagacact gtttctgcaa
    11881 ataaaaaagt ccgttccgac aagaacggca aagcagtgaa acaggacaaa aaatacaggg
    11941 aagagaaaaa tgccaaaacc gattccgacg aattgaaagc cgccgttgcc gctgccacca
    12001 atgatgtcga aaacaaaaaa gccctgctca aacaaagcga aggaatgctg cttcatgtca
    12061 gcaattccct caaacagctt caggaagagc gtatccgcca agaacgtatc cgccaagagc
    12121 gtatccgtca ggcgcgcggc aaccttgctt ccgtcaaccg caaacagcgc gaggcttggg
    12181 acaagttcca aaaactcaat accgagctga accgtttgaa aacggaagtc gccgctacga
    12241 aagcgcagat ttcccgtttc gtatcgggga actataaaaa cagccagccg aatgcggttg
    12301 ccctgttcct gaaaaacgcc gaaccgggtc agaaaaaccg ctttttgcgt tatacgcgtt
    12361 atgtaaacgc ctccaatcgg gaagttgtca aggatttgga aaaacagcag aaggctttgg
    12421 cggtacaaga gcagaaaatc aacaatgagc ttgcccgttt gaagaaaatt caggcaaacg
    12481 tgcaatccct gctgaaaaaa cagggtgtaa ccgatgcggc ggaacagacg gaaagccgca
    12541 gacagaatgc caaaatcgcc aaagatgccc gaaaactgct ggaacagaaa gggaacgagc
    12601 agcagctgaa caagctcttg agcaatttgg agaagaaaaa ggccgaacac cgcattcagg
    12661 atgcggaagc aaaaagaaaa ttggctgaag ccagactggc ggcagccgaa aaagccagaa
    12721 aagaagcggc gcagcagaag gctgaagcac gacgtgcgga aatgtccaac ctgaccgccg
    12781 aagacaggaa catccaagcg ccttcggtta tgggtatcgg cagtgccgac ggtttcagcc
    12841 gcatgcaggg acgtttgaaa aaaccggttg acggtgtgcc gaccggactt ttcgggcaga
    12901 accggagcgg cggcgatgtt tggaaaggcg tgttctattc cactgcaccg gcaacggttg
    12961 aaagcattgc gccgggaacg gtaagctatg cggacgagtt ggacggctac ggcaaagtgg
    13021 tcgtggtcga tcacggcgag aactacatca gcatctatgc cggtttgagc gaaatttccg
    13081 tcggcaaggg ttatatggtc gcggcaggaa gcaaaatcgg ctcgagcggg tcgctgccag
    13141 acggggaaga ggggctttac ctgcaaatac gttatcaagg tcaggtattg aacccttcga
    13201 gctggatacg ttgatcggat tgccgaaacg gtaggcttta tgcaaatcgt gccgatatgg
    13261 tgcgctgttc cgtgaagttt gccgtacctg tattttccgg tttttggaaa tcaagagaga
    13321 aagttaaaga agaatgtcga aacctgtttt taagaaaatc gcactttata ctttgggtgc
    13381 aatcagcggc gtggccgtca gtctggcggt gcagggtttt gccgccgaga aggacaggcg
    13441 ggataacgaa gtcctgccgg tgcaatccat ccgcacaatg gcggaggttt acggtcaaat
    13501 caaggcaaac tactatcagg acaaacccga tgccgatttg tttgaaggtg cgatgaaggg
    13561 tatggtggcc ggtttggatc cgcattccga atatatggat aaaaaaggtt atgccgagat
    13621 aaaggagtcc accagcggcg aatttggcgg cttggggatg gaaatcgggc aggaagacgg
    13681 atttgtcaaa gtggtttcgc cgattgagga cacgcctgcg gaacgggcgg gggtgaaaag
    13741 cggcgatttc attgtgaaaa tcgataatgt ttcgacgcgg gggatgacag tcagcgaagc
    13801 ggtgaagaaa atgcggggca agccgggtac gaagattact ttgacgctgt cgcgcaaaaa
    13861 tgccgacaag ccgatagtcg tcaacctgac ccgtgccatt attaaagtga aaagcgtccg
    13921 ccatcacctg atcgaacccg attacggcta tatccgcgtg tcgcagttcc aagagcggac
    13981 ggtcgaaagc gtcaataccg ccgcaaaaga gctggtaaag gaaaataaag gaaaaccgct
    14041 caaggggctg gtgttggatt tgcgcgacga ccccggcggg cttttgaacg gcgcggtcgg
    14101 cgtgtcggcg gcgtttttgc cgcctgatgc gctcgttgtc agtacgaaag ggcgggaagg
    14161 tacggaaggc tttgcgttaa aggcgcgtcc cgaagattat atttttaaag ccggcacgga
    14221 tcctttggcg ggtattcctg ccgagttgaa aaccataccg atgacggttc tgattaattc
    14281 gggttcggct tccgcgtcgg agattgtcgc aggtgcattg caggatcata aacgcgcggt
    14341 tatcgtcggt acgcagagct tcggcaaagg ttcggttcag actttgattc ctttgtccaa
    14401 cggcagcgcg gtcaagctga caacggcgct gtattatacg ccgaacgacc gttctattca
    14461 ggcgcagggg attgttcccg atgtcgaagt aaaagataag gaacgcattt ttgaaagccg
    14521 cgaggcggat ttggtcggac acatcggcaa tcctttgggc ggcgaggatg tgaacagtga
    14581 aacccttgcc gtgccgcttg aaaaagatgc ggataagccc gctgtaaaag aaaaaggtaa
    14641 aaagaaaaag gacgaggatt tgtcttcaag gcggattccc aaccctgcca aagacgacca
    14701 gttgcggaaa gctttggatt tagtcaagtc gcccgagcag tggcagaagt ctttggggct
    14761 ggcggcgaaa aagccggttt caaataaaga taagaaagat aaaaaagata agaagtaggt
    14821 ttttacgatg ccgtctgaaa ggatttcaga cggcatatta tgtttgaaag gacaggttat
    14881 gagcgatttt ttaccggaat ttgaaccgtg cggcatttat agtggattaa caaaaatcag
    14941 gacaaggcga cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac
    15001 tggtttttgt taatccacta tacttgggta gcgattgggt ttcagacggc atacattcag
    15061 gatttcaccg ccttatgccg tctgaaagac gtttccgacc catccctact gcacgctttg
    15121 tccgcaaacg catcacaggc aagattatgg aagttatcca atacccaaat tcccccttca
    15181 aactccacca acccttcccg cccgcaggcg accagcccac cgccattgca ggcctgctcg
    15241 aagggctttc agacggcctt gcctatcaaa ccctgctcgg cgtaaccggt tcgggcaaaa
    15301 cctacaccat ggcgaacgtc atcgcccaaa gcggccgccc cgccatcatc atggcgcaca
    15361 acaaaaccct tgccgcccag ctttacgccg aaatgcgcga atttttcccc gaaaacgcag
    15421 tggaatattt cgtctcctac tacgactatt accagcccga agcctatgtg cccagccgcg
    15481 atttgttcat cgaaaaagac agcgcgatca acgaacacat cgagcagatg cgcctttccg
    15541 ccaccaaaaa cctaatgacg cgcaacgacg tgattatcgt cgccaccgtg tccgccattt
    15601 acggtatcgg cgacccgacc gagtatcaac aaatggtgtt gtccgtcaaa gaaggcgata
    15661 ccatcgagca gcgcgacatc atcgccacgc tcgtttccat gcaatacgaa cgcggcgatt
    15721 tggacttcaa acgcggcagc ttccgcgtgc gcggcgacgt gattgacgtg taccccgccg
    15781 aaagctccga aaacgcctta cgcatcagcc tgtttgatga cgaaatcgac cgcctcgata
    15841 tgttcgaccc gctttcaggc agccttatcc agcgcgtcgg ccgctacacc gtcttcccgt
    15901 ccagccacta cgttaccccg cgcgacaccg tcttgcgcgc ctgcgagtcc atcaaagaag
    15961 aattacgcga acgcatcgaa tttttcgccc gcgaacaacg ccccgtcgaa caacaacgca
    16021 tcgaacagcg cacccgtttc gacctcgaaa tgctctacga aatgggcttc tgcaaaggaa
    16081 tcgaaaacta ctcccgccac ttttccggca aaaaagaagg cgaaccgccg cctacgctga
    16141 tggactacct gcccgacaac gccatcatgt tcatcgacga aagccacgtt accgtaaccc
    16201 aaatcggcgg catgtacaaa ggcgacgcat cgcgcaagca aaacctcgtc gactacggct
    16261 tccgcctgcc ttccgcccgc gacaaccgcc cgctcaaatt ccacgaattt gaaaaagtca
    16321 tgccgcaaac tgtcttcgtt tccgccactc cagccaaata cgaagaagaa cacgccggac
    16381 aagtggtcga acaagtcgtc cgtcccaccg ggctggttga tccccaaatc atcatccgca
    16441 ccgtcgccac ccaagtcgat gatttaatga gcgaaatcaa cgaccgcatc caaaaaggcg
    16501 aacgcgtgct cgttaccacc ctcaccaaac gcatggcgga gcaactcacc gactattaca
    16561 gcgaactcgg catcaaagtg cgctacctgc acagcgacat cgacaccgta gagcgcgttg
    16621 aaatcattag agatttgcgg ctcggactgt ttgacgtact cgtcggcatc aacctcttgc
    16681 gcgaaggctt ggacatcccc gaagtctccc tcgtcgccat ccttgatgcc gacaaagaag
    16741 gcttcctgcg ctcccaccgc agcctgattc aaaccatagg ccgcgccgcg cgcaacgtga
    16801 acggcgtcgc tatcctgtac gccgacaaaa tcaccgactc catgaaagcc gccatcgacg
    16861 aaaccgaacg ccgccgcgaa aaacagatga aattcaacga agaacacggc atcgtgccgc
    16921 agcagattaa aaaacaagtc aaagacatca tcgacggcgt gtaccacgaa gaagacggcg
    16981 gcaaaggccg tctgaaaggc aaaggtaaga acaaggttaa agtcggcgaa atccacaacg
    17041 aagaagacgc gattaaagaa atcgccaaac tggaaaaagc catgcaacag gcagctaggg
    17101 atttgcagtt tgaagaggcg gcggtgttaa gggataggat acgggggatt aaggaaaatc
    17161 tgttatttaa cgcaggataa tcgtaattag gagtattcaa tgaaactttt tgatgaaaaa
    17221 aaacgatatg aaaaatctga aattcggaaa aatcgatctt tatatgattt ttttgatgaa
    17281 tcttcattag aaaagtatga gaaaatcaga aatctattaa atgaggcttt gagtctgtat
    17341 gatgaagctt ctcaaaatag aataattgat gatattgtaa aaaataaaaa tagtgatgaa
    17401 aaattttact ctaactgctt tgaactaatt gtatcctata tgttcaggaa aattggtcaa
    17461 tatgaacttg agccacatcc tatattagct aataataaaa aacctgattt tttagttaca
    17521 gtccctaatt ttggtaaatt ttatttagaa ttagttactg ttgatgaaaa tgatatgggt
    17581 gatttgcagt ttaatatttt aaaaacatac attaaaaaat ttagaaatac aaatgaatta
    17641 tccaatgaag taactgttat ttctataaaa agtctaaatg gtagaaattt ctcccatgaa
    17701 aatgttatgg atttgcaaaa tgcaattaga gtttggtggg gagagaataa aaataatatt
    17761 ggtagcaaac tagaaaagga aatgaatgga aatttaattc attttgaaat tgttaaagaa
    17821 aatagtttaa gagtagaaag catcatagat gagaccgatt tctataaaaa attcttatcc
    17881 agcttagaat ataaatctaa aaaatatggg gagttagact taccatattc aattgcaata
    17941 acttttcgcc cttctaattt aacagatgga ttaattttac aagaaaattt tattgcttct
    18001 tgtttgtacg gtggatttta ttatgaacct aagagtgtta aaaaacgtaa gattcatagt
    18061 ctttgggaag tctttgaaga gaataaaaga aacaaccaag ttagtggtgt attatatttt
    18121 gattctttaa tccctgaaaa tttagaatct tttagatatt cactgttttt aaattattat
    18181 tcaaaatttg aattgccaca tgatttaaag agttttttac ccttttattg tgttgtaaat
    18241 aaaagtgaag cttttcaaag tgaaataatt gaaaaaggag atgtaatggc aagtggaagg
    18301 tttatttagg ctaccaagca agtcgcagca agcgcagatc ggatacttgt atccgacaaa
    18361 acctttaata ttcccgtcgt tgcaatccat tgcagcaatg cccaaaatgt cggattcgag
    18421 aatccgacct acaaaatcat tccgagcata atactatgaa ataccgtcgt ttttaccgca
    18481 acggcggcac ttgctttttt acggttgtaa ccaataaacg gcagaagatt ttgaccgatg
    18541 atgcggtgcg tttggctttg cggcaggcgg taatggcggt gcgcgaacgg tatccgtttg
    18601 aaattttggt atgggtgttg atgcccgacc atctgcatac catatggcgg ctgcggacaa
    18661 tgattctgct tattcggaac gctggcggca gattaagcgg cacagccaat atttaatcgg
    18721 cagcaatctc aggctttggc aaaaacgctt ttgggaatat actatccgcg atgaggccga
    18781 ttttgcctgc cattttgatt atctgcattt caatccggtc aaacatggct atgtaggaca
    18841 aatttccgat tggcggtttt ttacgtttca ccgttatgtt gaacagggta tttatccgca
    18901 taattggggt ggcggcaatg cggactttcc tattgaatac gattaaagca atgtcggatt
    18961 caggagccga cctacggaaa actaaagatt ttatacgggc tacggcttgc tggcttatgc
    19021 gttggcttaa tttgaaaaac agcacatcgg ttcataatac acaaggtatt agaacaaaag
    19081 aagcagttga acagggattg cgtggtaata atagacctga aaaaggaata ggtaatagat
    19141 ggaattatga tggatttata aaggaattag cgaagtaaca ggtattccaa atatttctga
    19201 taaatataaa gaacaaggag taaaaaaatg aaagtaatca ctattacatt gtttactagc
    19261 tgcatactat ctggatgcta tgggcttgaa tatggaatga gtggcggcat tcctcatgat
    19321 gactgggcaa gaggcagcca tgttagtcaa gccaaagcag atgctgcgtg gaaagaatgt
    19381 tctgacaaat atatgagaaa tggtggttca ggattagtag aacaatgtat gaatgataaa
    19441 ggattaatac gtttatctga aatacgtcgt aggcaaaaga gagcagaaac acaaagagca
    19501 gtaattaagt aacaataatg aaatactact gttcccgaac aaagcggcat ttacgcaggt
    19561 gatgacggct atattttgaa aaatgccgtc tgtaagtaaa cgtgcttcag acggcatttt
    19621 tttatccggc ggtctgttat ccgatccgtt tgaataccaa gtcccaaacg ccgtgtccga
    19681 ggcgtttgcc gcgcgcttcg aatttggttt cggggcggta gtccggggtg ggggcgtagt
    19741 ctgccgccgt attttgcagg ctgtcgaagc tgctgaggac ttcaagcatc tgctgtgcat
    19801 attcttccca gtctgtcgcc aggtggatat agccgccggt tttgagtttg ggcagcagtt
    19861 tggcgatgaa gggggcttgt atcagacggc gtttgtggtg gcgttttttg tgccacgggt
    19921 cggggaagaa tatgtggatg ccgtcgagcg agccgtcttg cagcatattt tcgacaactt
    19981 ctacggcatc gtgccgcatc acgcggatgt tttccaaatg gttttcgttg atgagtttga
    20041 gcaggttgcc tacgccggga ccgtgtacgt cgatggcgag gaagtcggtt tcgggcaggc
    20101 ggcgggcgat ttctgcggtt gctgtcccca taccgaagcc tatttcgagg attttggggc
    20161 ggttgcttcc gaaattggca tcaagatcga ccggtgttgc ttggtaatcg atgccgaagc
    20221 tgtcccataa ggtatcgatg gcgcgttgct gcgcggcggt catatggctt tggcggagga
    20281 cgaagctgcg gatgctgcgg ggatgtccgg cttgacggtc gtttggtgtt tggttttcgg
    20341 cggtgtctgt cataatgctt ccgtgcgctt gtgcaatcag gatgccgtct gaatgtgaaa
    20401 ggcctattcg gacggcggga agggaaaggg gcgattatac ctgtttcgtt tcggcagggt
    20461 aggtgtaacg ctgttcagac ggcattttgc cattgttgca gcagttcttg caggacgctt
    20521 ttttgcccgt atccgttgtt tatggcttct tgcaggtagc ggcaggcttt ttctttgtcc
    20581 ggctcgacgc cgtgtccgtt gtaatggatg caggcgaggt tataaagtgc catgctgtct
    20641 ttttgcgcgg cggctttttc aaaccatttg cgtgcttcat tgtagtcggc ggtcataccc
    20701 tgtccgaagt aatacatgct gcccagattg gtttgggctt tgctgtgtcc gagttctgcg
    20761 gcttctgtgt agagtttgag cgccgccgcg tattcgggcg cgcattgtaa tccgtaatgc
    20821 agggcttggg ctttttgata aagctgttct gccctttgcc gcctaatggc ggaatcttta
    20881 atgccgccgt attggtcagg atggtttaac gcgctgtcgg atatgagttt ctgataggcg
    20941 gtaaggctgc cggcttcggc tgcctgccgg taataatgcc gggctttttc cgtatcgggg
    21001 gctatgccca aaccgtagcg gcagatgtcg cccagtatcc gcaaaccttc gggatggcgt
    21061 tcggcggcgg ctgcttcggc gtgatgcagg gcaagtttgt agtttctttc cggatgcttg
    21121 ccttgcaggt agatgtcggc aagtgcggca tgggcatcgg ggtcattttc ctgtgcggcc
    21181 tgttgaaacc ggtaaacggc tttatcggga gccgtaggta ggatgagctc ggcaagcggg
    21241 gtgtaggcgg gaatgtatcc ttgttgtgcg gcggcgcgca aatgattgat agcttgggtc
    21301 gtgtcggttg gtgtaccttg tccgaaacgg tattgaaggc cgagctgcca ttgtgccgcc
    21361 gccaagcctt gtgctgcagc ctcgcggtag agaaggtgtg ccgtttcaaa atccggcggc
    21421 gtgccgtacc ggcagtattg ggcgagcagg tattgcccgt ggggattgtt gttttttgtg
    21481 cttgtttttg cccattccaa ggcttcttca aaatgtcggg acgcatacag caggtacatc
    21541 aggcggactt gtgcttcagg cagtctttct tctgccaacg gtctgtaaag cggtatcagg
    21601 gtttccgggg gcgcgccttc caattcttgg atatatgcca gattgtaacg cgcataggga
    21661 tgcccctgtt cggcggcgat gcggagcatt tccatacctt gttcgcggtc ttggtagggg
    21721 ctgtccgcgt ccatcaggtg ttttgccaac tcgaatgcgg cggcgggatg acctgccgag
    21781 gccgcttctg tcagcagtcg gcggacttcg gtaaagttat attgtttttg agacaggcgt
    21841 tccaaggcct gccgataggg taagtcggga ttgttcatat cagatcgttt tccgttttgt
    21901 cgaattatcg ttattcgtta tgaagttagg tttatatcgg cacgccgacc tactgtatca
    21961 tattttttac agacggtttt tgctttggcg gcatactaat gcagatgatt gtaaatggtt
    22021 tccgccaatg ccttgctgat gccttccact tgttccaaat cctcgcggct ggcggcaatc
    22081 acgccgcgca ggccgccgaa gcgggtgagc agggcttggc ggcgtttgct gcctacgccg
    22141 gggatgtcgc ttaaggagga ggtaacgcgg gctttgtcgc gttttttgcg gtggccggta
    22201 atggcgaaac ggtgcgattc atcgcgtacg gtttgcaata aatgcaaggc cgggctgtgt
    22261 gggggcaggc ggaagacttc gccggtaaaa ggcagtatga gctcctccat accggctttg
    22321 cgctccgggc ctttggcaat gccgaccaaa gggatgtgca gcccgagttc ttcccatacc
    22381 gatacggcta cgccgatttg ccctttgccg ccgtcaatca acacggcatc cggccatttg
    22441 acggtctcgc cgttggcttc ggcttcctgc attttgccgt aacggcgcgt caacacttcg
    22501 cgcatggcgg cgtagtcgtc gccgggtttg gcggtcgtga tgttgtagcg gcggtattgc
    22561 gagggctgga tgttttgctc atcgtacacg acgcaggagg cgatggtggc ttcgccttgg
    22621 gtgtggctga tgtcgaagca ttcgaggcgg ttgaggccgt ctgaatccat accgaggatt
    22681 ttggcgagtt cgtcgatgcg gtgttgctgg ttgctttgtt gcaggcggcg ttgtgcaatc
    22741 gccatttgtg cgttttgttc cgccattttc aaccaaactt tgcgttcgcc tatggtcttg
    22801 gtgacaaatt gcatttgttt gccgtgttcg ccttccaaag cctctttcag cgcatcggga
    22861 acggaaaagt tgctaataat gatgtcgggt ttgcttttgc ccaaataatg ttgggcgacg
    22921 aaggcttcgg cgtaatcttg tccgtttggc tcgggatcgt ttttggtgtc ggggaagaaa
    22981 cttttgtctc cgacgtgccg cccaccgcgt atgctgaccc agtgtacgca gacgatgccg
    23041 tctgaaaccg ccagcgcgag caaatcgata tcgttggggt tgttcgggtt tttgctgtcg
    23101 atgaactggt tgctctgcat aatgccgagt gcttggattt ggtcgcggta gcgggcggct
    23161 tcttcaaatt gcagattcgc ggcggcagtt tgcattttgt gttgcagggt gcgggtcagt
    23221 tcgtcggttt tgccattgag aaaagtggcg gcttgacgca cgctgtcgcg gtagtcttct
    23281 tcgctgatgt gtccgacgca gggcgcggtg cagcgtttga tttgatacag caggcaaggg
    23341 cggtcacgat gctcgaatac gctgtcttcg caggtacgca gcataaagac tttttgcaac
    23401 acttgaatgc tgtcgcgcac ggcgttgctg ttgggatacg ggccgaagta ttggtttggc
    23461 tttttcagcg tgccgcggta atacgccatc tgcggatatt gatggccgct gagcatcaaa
    23521 taaggatagc ttttgtcgtc gcggaaaagg atattgtatt tcggcgacaa ggctttgatg
    23581 aggttgtttt cgagaatcag ggcttcggct tcggaacgcg tgatggtggt ttcgatatga
    23641 tgaacctgtt ttaccatcaa tgcgatgcgc ggcgaatggt cgtttttttg gaaatagccg
    23701 gacacgcgcc gcttgaggtt gaccgctttg ccgacgtata agactttgcc gccttcgtca
    23761 aaaaaacggt acacgcccgg cagcttgggc aggtttttga ggaagagcgg gatatcgaat
    23821 tgttccggaa aacgggtttc tttattcata tcaattactt gaaaaacggt ttcagacggc
    23881 atattaccat atcgctggag acagccttat ccgttcggac aaggctgcag ggcggttaca
    23941 tgctgctaat cgatttttac ccgtttcagg cgcaaggcat tgctcaacac ggaaaccgag
    24001 cttgccgcca ttgccgcgcc agcgatgacg ggatttaaaa agccaagcgc ggcgagagga
    24061 atgcccaaaa tattgtagaa gaaggcgaaa aacaggtttt gcttgatgtt tttcaaagtg
    24121 gcttgcgaca ccagcagagc atcggcgagt tggttgaccg aatgctgcat cagcgtggcg
    24181 gatgcggtat gttcggcaac gtccgctccg cctttcatgg cgaagctgac gttagcggcg
    24241 gcaagcgcgg gcgcgtcgtt gatgccgtcg ccgaccatcg ccacggtttt gccggcggct
    24301 ttgagttttt gcacttcggc agctttatcg cgcggactca tgttgccgaa ggcgtgtgcg
    24361 atgcccagtt gtttggcgac gtattcgacc gtgccttggt tgtcgccgct cataatatag
    24421 acatcgatat tgtgtttttt cagacggcct atggcttcgg cggtatcggc tttcaacgcg
    24481 tcggcaagtg cgaatgcgcc gatgggtttg ttatcgactg agaccgcaac aatgcttgca
    24541 atatcccaaa cgccgtctaa aaacttcggc aaggccagtt cggcaaattc ggctttgcct
    24601 gctttcacca aacccacgcc ttccacttcg gcggtaatgc ctgcgccgac aacggtttgt
    24661 gcgttttgtg cggcgggaat gtccaaaccg cgcgcttggg cggcggagac gatggcacgg
    24721 gcgagcggat gggcggcgtt ttgttcgacg gcggcggcga tgcggtacaa agcgtcttcg
    24781 tcaaagccgc tgtcgggaac gcaataaacg gcggcaacct gcgggctgcc ttcggtcagc
    24841 gtaccggttt tgtccaacac gacggcatcg acgtgggcgg cttcctccat tgctgccgcg
    24901 tctttaaacc aaataccgtg tttaaccgct ttgcccatac cgaccataat cgcggcaggg
    24961 gttgccagac ccagcgcgca cgggcaggca atcaccaaaa cggcgacggc gtgcatcagc
    25021 gcaaccgtcc aatcgccctt aatcagccaa gtaacaataa aagtcaacaa cgcaatgccc
    25081 acgacggcag gcacgaatac cgcagccgct ttatcggcta cgcgcgcaat cggtgcttta
    25141 ctgccttgtg cttcagagag cgcgttcatc atgtcgccga gctgggtttg gctgccgagc
    25201 tgcgtggcgc ggtacaccac actgccttcg gtcattaacg cgcccgccaa cactttgccg
    25261 cccgcctttt tttcttcagg attggattcg ccggtaagat ggctctcgtc cgcccaaccg
    25321 ctgccgcttt caatgatgcc gtctgcggca atgcgttcgc cgtggttggc gcggataagg
    25381 tcgccgattt gcacttggtc gatgggaagc tgtttccatt cgccgttgcg ttgcacgttg
    25441 acttgggttg gtgtaagttt gagcagcaag cccaagctgt tgaggctgga ttttttggta
    25501 cggtgttcca aaaatttacc cagtgacaca aaaccgatca ccatcacgcc cacttcaaaa
    25561 tacacatgcg ccataccgta cgccgcgtgc gggctgaaaa acagcatata gacggaatac
    25621 aggtaaatcg agaccgtgcc gatggtaacc agcacgtcca tattcgccag tccgccctta
    25681 atgctcgccc acgcgctttt gtaaaacggg attgccagcc aaagctgcac cacgcttgcc
    25741 aatgcgaact gccacaacgg cggaatcatc caatcgtgtc tgccgatcat catccccgcc
    25801 atgccgataa ggaacgggac gttgatggtg a
//
LOCUS       DAWHRS010000008        25431 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWHRS010000008 DAWHRS010000000
VERSION     DAWHRS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.008"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            241..366
                     /locus_tag="WIB82_000245"
                     /pseudo
     CDS             241..366
                     /locus_tag="WIB82_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(517..1560)
                     /locus_tag="WIB82_000246"
     CDS             complement(517..1560)
                     /locus_tag="WIB82_000246"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ3841614.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFVDI
                     LPTGSAPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGAIPLLP"
     gene            complement(1557..1838)
                     /locus_tag="WIB82_000247"
                     /pseudo
     CDS             complement(1557..1838)
                     /locus_tag="WIB82_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2092..3246
                     /locus_tag="WIB82_000248"
     CDS             2092..3246
                     /locus_tag="WIB82_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ3841615.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3475..3828
                     /locus_tag="WIB82_000249"
     CDS             3475..3828
                     /locus_tag="WIB82_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841616.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3889..4605
                     /locus_tag="WIB82_000250"
     CDS             3889..4605
                     /locus_tag="WIB82_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ3841617.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            4670..5113
                     /locus_tag="WIB82_000251"
     CDS             4670..5113
                     /locus_tag="WIB82_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ3841618.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKSTITAYGEEKPLQIGMTLEADILHEKR"
     gene            complement(5362..5469)
                     /locus_tag="WIB82_000252"
                     /pseudo
     CDS             complement(5362..5469)
                     /locus_tag="WIB82_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            5479..5781
                     /locus_tag="WIB82_000253"
     CDS             5479..5781
                     /locus_tag="WIB82_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841619.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            6065..6304
                     /locus_tag="WIB82_000254"
     CDS             6065..6304
                     /locus_tag="WIB82_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ3841620.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            6365..8107
                     /gene="hrpB"
                     /locus_tag="WIB82_000255"
     CDS             6365..8107
                     /gene="hrpB"
                     /locus_tag="WIB82_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ3841621.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDVNVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NSWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            8216..14260
                     /locus_tag="WIB82_000256"
     CDS             8216..14260
                     /locus_tag="WIB82_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ3841622.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFLVKGGAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTEILARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGSANLSAGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKSG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LAKQPEYAYLKQLQVAKNVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTSGAG
                     TGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKELGRSSTVKNLVVAAA
                     TAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANILAA
                     LVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIVGEA
                     LTNGKNPATLTAKEREQILAYSKLVAGTVSGVVGGDVNTAANAAKVAIENNLLSQEEY
                     ALREKLIKKAKGKGLLSLDWGSLTEQEARQFIYLIEKDRYSNQLLDRYQKNPSSLNNQ
                     EKNILAYFINQTSGGNTAWAASILKTPQSMGNLTIPSKDINNTLSKAYQTLSRYDSFD
                     YKSAVAAQPALYLLNGPLGFSVKAATVAAGGYNIGQGAKAISNGEYLHGTVQVVNGTL
                     MVAGSVSAQAAISAKPAPVTRYLSNDSAPALRQALTAESQRIRMKLPEEYRQIGNLAI
                     AKIDVKGLPQRMEAFSSFQKGEHGFISLPETKIFKPISVDKYHNIASPPRGTLRNIDG
                     EYKLLETIAQQLGNNRNVSGRIDLFTELKACQSCSNVILEFRNRYPNIQLNIFTGK"
     gene            14265..14648
                     /locus_tag="WIB82_000257"
     CDS             14265..14648
                     /locus_tag="WIB82_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841623.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            14898..17561
                     /locus_tag="WIB82_000258"
     CDS             14898..17561
                     /locus_tag="WIB82_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ3841624.1"
                     /translation="MLKTPPMLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVTLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAGAA
                     IVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASLYS
                     QAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELF
                     SSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAA
                     SKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATL
                     SDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQ
                     KPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGW
                     TAPLIGTAGKAAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKA
                     KAAKQAIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQPVSAGFEHV
                     LEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDVGKVIGTT
                     SIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            17568..18077
                     /locus_tag="WIB82_000259"
     CDS             17568..18077
                     /locus_tag="WIB82_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841625.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            18137..19825
                     /locus_tag="WIB82_000260"
     CDS             18137..19825
                     /locus_tag="WIB82_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ3841626.1"
                     /translation="MRSMIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLF
                     EAPRLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNVN
                     WNQVQLAYDKWDYKQEGLTRAGAAIIALAVTVVTAGAGVGAALGLNGAAAAAADAAFA
                     SLASQASVSLINNKGDVGKTLKELGRSRTVKNLVVAAATAGVSNKLGASSLATWSETP
                     WVNNLNVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHY
                     IAHKVAHAVAGCAAATANKGKCQDGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVK
                     KITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNG
                     KINVRDLKQTLKDEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQK
                     VKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQ
                     GLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            19834..20211
                     /locus_tag="WIB82_000261"
     CDS             19834..20211
                     /locus_tag="WIB82_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841627.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            20489..20863
                     /locus_tag="WIB82_000262"
     CDS             20489..20863
                     /locus_tag="WIB82_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841628.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            20898..22487
                     /locus_tag="WIB82_000263"
     CDS             20898..22487
                     /locus_tag="WIB82_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ3841629.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGNIGNTLKELGRSSTVKNLVV
                     AAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYIAHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIL
                     GETLLDGRDPGSLNVKDRAKIIAKAKLAAGAVAALSKGDVSTAANAAAVAVENNSLND
                     IQDRLLSGNYALCMSAGGAESFCESYRPLGLPHFVSVSGEMKLPNKFGNRMVNGKLII
                     NTRNGNVYFSVGKIWSTVKSTKSNISGVSVGWVLNVSPNDYLKEASMNDFRNSNQNKA
                     YAEMISQTLVGESVGGSLCLTRACFSVSSTISKSKSPFKDSKIIGEIGLGSGVAAGVE
                     KTIYIGNIKDIDKFISANIKK"
     gene            22496..22918
                     /locus_tag="WIB82_000264"
     CDS             22496..22918
                     /locus_tag="WIB82_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841630.1"
                     /translation="MKYMISFLKKTFELMSWVLVILIIGTFYDYYQIRQYAELEKKSI
                     SNILLYAQKEKFRLESKDKYMRGGYTKYKFIFSEYSNTTFLNFINDLKKDNYLPLDGY
                     GHGFLCRKGESISINIYPEINKFILVWGYPENLCADSN"
     gene            22955..23356
                     /locus_tag="WIB82_000265"
     CDS             22955..23356
                     /locus_tag="WIB82_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ3841631.1"
                     /translation="MRKLNNHDVHKRYQDRLEEDVEFTINYELPLSCLWSTIKDFSSD
                     FEEKTEAFFILFKELLRRGHLKLQRDGQIIGHTPEEWEQIFREVWPEYEIEPNPLPGY
                     APFDIGMWLTVEAPAYAVWIDPEDGSEYWAG"
     gene            23479..24099
                     /locus_tag="WIB82_000266"
     CDS             23479..24099
                     /locus_tag="WIB82_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841632.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKTISEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GANNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIKQVITNLPD
                     NHPTKNILREAVRSGKIKTAVTGVDRQTGKAVILPVKVPSKTNIRR"
     gene            24102..25040
                     /locus_tag="WIB82_000267"
     CDS             24102..25040
                     /locus_tag="WIB82_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ3841633.1"
                     /translation="MGHNMMTTQKWYEHITDVIIDTTQRFNREKSADSIEYINERKGS
                     PIGAICVVSIDVYAACSHAYLFEHNLKKFKQYAYAYSKLEILASMGWSDPTPFFFPCD
                     MLNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFINRYDLNRHMIYNTLLMVEGK
                     QLDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARM
                     AAKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPADEYQDIFD
                     FMKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT         7706 a         5567 c         5955 g         6203 t
ORIGIN      
        1 ggaatgacgg gattttaggt ttttggtttg ttttcgcggc aatgacggga ttttaggttt
       61 ttggtttgtt ttcacggcaa tgatgggatt ttaaattgca ggtatttacc gggaaaaacg
      121 gcaagccctc cgccgtcatt cccgcgcagg cgggaatcta gaaatgaaaa accacggtgt
      181 tatcggcaat gactgaaacc ggagaaatga cgaaattgaa gcgtgtggaa atgtgtcgga
      241 aatcgctgaa gccggataaa ctatagtgga ttaaatttaa atcaggacaa ggcgacgaag
      301 ccgcagacag tacagatagt acggcaaggc gaggcaacgc tgtactggtt taaatttaat
      361 ccactataga ttcccgcttt tgcgggaatg acggtatttt agatttttgt tttcgcggaa
      421 taatgagcca aaagccgtcc gaacccaaaa aaaccgtcgc tttcagacgg catcaaaaaa
      481 ccgcaggcac gatgcctgcg gtttgccgtt tgcgcttcag gggagcaggg ggattgcgcc
      541 caagccgtgt gtttcctcca gtccgaacat aatgttcata ttttggactg cctgacccgc
      601 cgcgcctttg acgaggttgt cgatgacgga aaggacgacc cacacatcgg attgcgccgc
      661 ctgttggatg ctgatgcggc agaggtttgc gccgcgcacg ctgcgggttt cgggggcgga
      721 accggtcggc aggatgtcca cgaacgggct gtcgcggtag tagtcgcgca ggacggtttc
      781 ggggtcgctg ccgtctgaaa ggtggaggta aacggtggcg tgcataccgc gtatcattgg
      841 cgcgaggtgc ggcgtgaaca cgaatccttc ggcgatgccg tcctgaagcc cggcgatggt
      901 ctgcctgatt tcgggcaggt ggcggtgtcc ggctatgccg taggctttga agttgtcgcc
      961 ggcttcgcac aacagcgaac cgacattgcc tttcctgccc gcgccggaca caccggattt
     1021 gcagtcggca atcagcggca taccgggctt cagacggcat tgccgcaaca gcggcacgag
     1081 cggtagggat acgcaggtcg ggtagcagcc ggggttggcg acgaggcgcg cctgtgcgac
     1141 ggcttcgcgg ttgagttcgc tcaatccgta cacggcttgg gaaacgaggt cgggggcggc
     1201 gtgggtcatg ccgtaccagt gttcccaggt cggaatgtcc cgtatgcgga agtcggcgga
     1261 aaggtcgatg acgcgcacgc cctgttcaat caggcgcggc gcgtctttca tggcgatgcc
     1321 gttgggcgtg gcgaagaaga cgatgtcgca ttgttccaaa cctgcctcgt cgggcgtttg
     1381 gaaggcgagg ccgtacacgc cgcgcaaact cggaaagtaa tcggcaactg cggttcccgc
     1441 ttcgctgcgg ctggttacgg cggcgacttc gacatcggga tgggcggcaa gcaggcgcag
     1501 cagttccacg ccggtgtagc ccgtcgcgcc gacaatgccg actttgattt ttttactcat
     1561 ggtttttcct ttgtgtggtt ggcgggtatg ccgtccgaac gctgtccgac ggggttttga
     1621 ttggcggcgg aaggacggta cacaaagcgt ttgaaaagcg tgtctaattt aatggcatcc
     1681 gcctctgttt ttaaaaccta gccctgccgt ccggaataaa cataaccgta ggcgcgaaag
     1741 ccggcaggat atttcggtgc ctgccccaaa cccaaaccgt atgcggcgaa agcggtggtc
     1801 gtggagaagg aaagcaggag gaggacgagg gttttcataa ggttccaaac cgaacggggc
     1861 gatgaagccc gattatagca aaaggtcgtc tgaaaaccgt tttcagacga ccttttattt
     1921 aattacaata aaaatgttaa caacaaaaaa caaaccgctt ttttccgttt ggagacaaat
     1981 ttttaactaa aattgtcaac aattccttga cgcacacaca aaaaaaactt taatatttgt
     2041 aaatatatgt aacattataa attttaaata ttttctgagt aaggggaagt aatgagcatc
     2101 catctcgact tcggcattag tcctaaaacg ttccgacaga cttatctgta tcaaaagccc
     2161 aagctcttta aaggagcggt tcggaatctc gaatccgcat cttggaaaga tatcaacgag
     2221 atataccaac gagcagaccc aaccgcaccg ctgtttcatc tgcgtaaaaa aggcgcaatc
     2281 gttcctaaag aagaatacgt cgaaagtttc gacgatttgg gcaaaactcg ctaccgtttt
     2341 attaaatccg ttatctacga acatatgaag aatggtgcgt cgttagtcta taaccatatt
     2401 aacaacgagc cgttttcaga ccatatcgcc cgtcaagtcg cccgctttgc cggcgcacat
     2461 actattgtta gtggatatct tgcttttggc agcgacgaat cttataaaaa ccattgggac
     2521 ccccgcgatg tgtatgccat ccagcttttc ggcaagaaac gttggcaact tactgcccct
     2581 gatttcccta tgccattgta tatgcaacag actaaagata ctgatatttc cattcctgaa
     2641 catatcgata tggatattat ccttgaagca ggtgatgtcc tctacatccc acgcggttgg
     2701 tggcacagac ctatcccgct cggctgtgaa accttccact tcgctgtcgg tacctttcca
     2761 cccaacggct ataattacct cgagtggcta atgaagaaat ttcccaccat agaaagtctg
     2821 cgccacagtt tctcagactg ggagcaagat aggacgcgta tcaacgatac tgccgcacaa
     2881 attgctgcca tgattgccga ccccgtcaat tatgaagcct tcagtgaaga ctttctcggc
     2941 aaagaacgta ccgataccgc ttttcatctc gaacagttcg cgaatcccaa cgctactccg
     3001 ctttcagacg acgtcaggtt gagattaaat gccaataatt tggatacgtt ggaaaaggga
     3061 tatttgattg ggaatgggat gaagataagc gtagatgagt tggggaaaaa agtgttagaa
     3121 cacatcggta agaatgaacc gttattgttg aaaaatctac tggttaactt caatcaggga
     3181 aaatatgaag aagttaggaa gttgatttat cagttgatag agttagattt tctggaaatt
     3241 ttgtgaggga ttctatgaaa aactggaagc aatttacatt tttcgtaata ttagtaatag
     3301 cttgttatca agtgctatat tttttatcag atatgtttct tctcgactac attaacaaat
     3361 atagttggaa tttgaatttt attcagggta ctttgaattt tttttcaata tatctgccat
     3421 atgtttttgt tagccgaatt tttaggaata ccaaccaaga aaaggagtat aaaaatgatt
     3481 gaacttcaac ttcatgaatt gaagctggtt tcagggggag gtcctgtaac agacaatata
     3541 gctggaaatg tagctaatgc tgccacaacc aaaggaggtc ccacatgggg ggatttggtt
     3601 gcaatacctg ctgccgcagc ggcagtttat tatctgccga aaaatgctta cggtgctgct
     3661 ggtgcaaatg gtgtatacaa tgtgactcgt aattgggtaa atgatgccgt taatgcacct
     3721 ccttataacg gaagaccaat ctttgagatt gaacatggat taactgctcc cgcaacaaaa
     3781 gcagataaat caggaaacgg ctacactgac ggtacagatt actgctgata tttctcatcg
     3841 tccagacaat ctctaggggt cgtctgaaac tttcttaact tcaattttat gaatagaccc
     3901 aagcaaccct tcttccgtcc cgaagtcgcc gttgcccgcc aaaccagcct gacgggtaaa
     3961 gtgattctga cacgaccgtt gtcattttcc ctatggacga catttgcatc gatatctgcg
     4021 ttattgatta tcctgttttt gatatttggt aactatacgc gaaagacaac agtggaggga
     4081 caaattttac ctgcatcggg cgtaatcagg gtgtatgcac cggatacggg gacaattaca
     4141 gcgaaattcg tggaagatgg agaaaaggtt aaggctggcg acaagctatt tgcgctttcg
     4201 acctcacgtt tcggcgcagg aggtagcgtg cagcagcagt tgaaaacgga ggcagttttg
     4261 aagaaaacgt tggcagaaca ggaactgggt cgtctgaagc tgatacacgg gaatgaaacg
     4321 cgcagcctta aagcaactgt cgaacgtttg gaaaaccagg aactccatat ttcgcaacag
     4381 atagacggtc agaaaaggcg cattagactt gcggaagaaa tgttgcagaa atatcgtttc
     4441 ctatccgcca atgatgcagt gccaaaacaa gaaatgatga atgtcaaggc agagctttta
     4501 gagcagaaag ccaaacttga tgcctaccgc cgagaagaag tcgggctgct tcaggaaatc
     4561 cgcacgcaga atctgacatt ggccagcctc ccccaagcgg catgagacag aacaaagctg
     4621 gcttgaacgc accattgccg atatttctca agaagttttg gattttgaaa tgcgctctga
     4681 acaaatcatc cgtgcaggac ggtcgggtta tatagcaata ccgaacgtcg aagtcggaca
     4741 gcaggttgat ccttccaaac tgctcttgag cattgttccc gaacgtaccg agctatatgc
     4801 ccatctatat atccccagca gtgcagcagg ctttatcaag ccgaaagaca aggttgtcct
     4861 acgttatcag gcatatccct atcaaaaatt cgggcttgct tccggcagtg tcgtatcagt
     4921 agcaaaaacg gcactgggca gacaggaatt gtcgggattg ggcatggtat cctccgattt
     4981 ggcgaagagc aacgaacctg tttatcttgt gaaaataaaa cccgacaaat caaccatcac
     5041 tgcatacggt gaggaaaaac cgctgcaaat cggcatgacg ttggaagcag acatcctaca
     5101 cgagaaacgg tgattgtacg aatgggtatt ggagctgatt tatagtatgt cgggcaaact
     5161 gtaagacttg aggctgtaaa gataaagaag ttgtctgaaa ccttttcaga cgaccagaag
     5221 acaatatata aaaacacaaa cttcaaaact gcctgaaacc atcccctttt acaggcactc
     5281 cgtttgtaaa agcaaggata gggcatctgc ccttttgatt gtttgattaa cgatacaagg
     5341 agtttcaaaa tgagagtttt atagtggatt aaatttaaac cagtacagcg ttgcctcgcc
     5401 ttgccgtacc atttgtactg tctgcggctt cgtcgccttg tcctgattta aatttaatcc
     5461 actatatgtg ttcatgaaat gacttgggtc ggaggctcag gtaatgcgca acaaagctca
     5521 tattattgcg aaatttgcga atctgcaggg cttaacgata cgggaaatcc tgataaatct
     5581 ttaggattgc caaacaatac gttcagtaat ccgcctggtt ggggagctac aatcggagct
     5641 ttagcaggta gccgcatagg gatgcctgaa tttggtacgt ttgcgagcca tgccattgaa
     5701 aatttcgact ggtcatggta tcgacgttat agggaaattg ccgaaacgat tgaacgagaa
     5761 tattcaggcg gtttgcctta atagttgagg aggtcatgat gtttgccaaa cattatcaat
     5821 tcatcgcact cggcatcatg ctgcttcttt atatgttgat tctctatacg accgattttt
     5881 ccaatctgac gtattggatg ctgtttttta tctgttttat tacaggaaaa atattagctc
     5941 gtttgttaga gaaaagcttt aaataaaata gcagctagtc gcaaaaggtc gtctgaaacc
     6001 ttttcaggcg gcctttctaa aatacatcca acttcctaat ccctattttt caaaaaggaa
     6061 atctatgccc catctgcaaa acctgtcttt gggcttaaag aaaaagctgc ctgttatcct
     6121 gcaaacagaa atatcagaat gcggcttggc atgtctggcg gctgtggcgg gatttcatgg
     6181 tttccatacg aatttacgcg cactgcgttc aaaatactgt ccgagacctt tgcaaaattc
     6241 cccaaaatcc cctaaatgtc ttggtgggaa ttttggggaa ttttgcaaag gtctcattct
     6301 ataactgtaa atacttttaa atttatgaca aaatagtaaa tattgctaaa ataatattga
     6361 tgtcatgaaa ttttttcctg ctccatgtct gttggttatc ctggctgtca taccccttaa
     6421 aaccttagct gccgatgaaa acgatgcaga acttatccgt tccatgcagc gtcagcagca
     6481 catagatgct gaattgttaa ctgatgtaaa tgtccgtttc gagcaaccat tggagaagaa
     6541 caattatgtc ctgagtgaag atgaaacacc gtgtactcgg gtaaattaca ttagtttaga
     6601 tgataagacg gtgcgcaaat tttcttttct tccttctgtg ctcatgaaag aaacagcttt
     6661 taaaactggg atgtgtttag gttccaataa tttgagcagg ctacaaaaag ctgcgcaaca
     6721 gatactgatt gtgcgtggct acctcacttc ccaagctatt atccaaccac agaatatgga
     6781 ttcgggaatt ctgaaattac gggtatcagc aggcgaaata ggggatatcc gctatgaaga
     6841 aaaacgggat gggaagtctg ccgagggcag tattagtgca ttcaataaca aatttccctt
     6901 atataggaac aaaattctca atcttcgcga tgtagagcag ggcttggaaa acctgcgtcg
     6961 tttgtcgagt gttaaaacag atattcagat tataccgtcc gaagaagaag gcaaaagcga
     7021 tttacagatc aaatggcagc agaataaacc catacggttc agtatcggta tagatgatgc
     7081 gggcggcaaa acgaccggca aatatcaagg aaatgtcgct ttatcgttcg ataacccttt
     7141 gggcttaagc gatttgtttt atgtttcata tggacgcggt ttggcgcaca aaacggactt
     7201 gactgatgcc accggtacgg aaactgaaag cggatccaga agttacagcg tgcattattc
     7261 ggtgcccgta aaaaaatggc tgttttcttt taatcacaat ggacatcgtt accacgaagc
     7321 aaccgaaggc tattccgtca attacgatta caacggcaaa caatatcaga gcagcctggc
     7381 cgccgagcgc atgctttggc gtaacaggtt tcataaaact tcagtcggaa tgaaattatg
     7441 gacacgccaa acctataaat acatcgacga tgccgaaatc gaagtgcaac gccgccgctc
     7501 tgcaggctgg gaagccgaat tgcgccaccg tgcttacctc aacagttggc agcttgacgg
     7561 caagttgtct tacaaacgcg ggaccggcat gcgccaaagt atgcctgcac cggaagaaaa
     7621 cggcggcgat attcttccag gtacatctcg tatgaaaatc attactgccg gtttggacgc
     7681 agccgcccca tttattttag gcaaacagca gtttttctac gcaaccgcca ttcaagctca
     7741 atggaacaaa acgccgttgg ttgcccaaga taaattgtca atcggcagcc gctacaccgt
     7801 tcgcggattt gatggggagc agagtctttt cggagagcga ggtttctact ggcagaatac
     7861 tttaacttgg tattttcatc cgaaccatca gttctatctc ggcgcggact atggccgcgt
     7921 atctggcgaa agtgcacaat atgtatcggg caagcagctg atgggtgcag tggtcggctt
     7981 cagaggaggg cataaagtag gcggtatgtt tgcttatgat ctgtttgccg gcaagccgct
     8041 tcataaaccc aaaggctttc agacgaccaa caccgtttac ggcttcaact tgaattacag
     8101 tttctaacct ctgaattttt tactgatatt tagacggtct ttccttatcc tcagaccgtc
     8161 aaactttacc tacgtacttg gcgcgcagta cgttcatctt caaaatggaa tagacatgaa
     8221 taaaggttta catcgcatta tctttagtaa aaagcacagc accatggttg cagtagccga
     8281 aactgccaac agccagggca aaggtaaaca ggcaggcagt tcggtttctg tttcactgaa
     8341 aacttcaggc gacctttgcg gcaaactcaa aaccaccctt aaaaccttgg tctgctcttt
     8401 ggtttccctg agtatggtat tgcctgccca tgcccaaatt accaccgaca aatcagcacc
     8461 taaaaaccag caggtcgtta tccttaaaac caacactggt gcccccttgg tgaatatcca
     8521 aactccgaat ggacgcggat tgagccacaa ccgctatacg cagtttgatg ttgacaacaa
     8581 aggggcagtg ttaaacaacg accgtaacaa taatccgttt ctggtcaaag gcggtgcgca
     8641 attgattttg aacgaggtac gcggtacggc tagcaaactc aacggcatcg ttaccgtagg
     8701 cggccaaaag gccgacgtga ttattgccaa ccccaacggc attaccgtta atggcggcgg
     8761 ctttaaaaat gtcggtcggg gcatcttaac taccggtgcg ccccaaatcg gcaaagacgg
     8821 tgcactgaca ggatttgatg tgcgtcaagg cacattgacc gtaggagcag caggttggaa
     8881 tgataaaggc ggagccgact acaccgagat acttgctcgt gcagttgctt tgcaggggaa
     8941 attacagggt aaaaacctgg cggtttctac cggtcctcag aaagtagatt acgccagcgg
     9001 cgaaatcagt gcaggtacgg cagcgggtac gaaaccgact attgcccttg atactgccgc
     9061 actgggcggt atgtacgcag acagcatcac actgattgcc aatgaaaaag gcgtaggcgt
     9121 caaaaatgcc ggcacactcg aagcggccaa gcaattgatt gtgacttcgt caggccgcat
     9181 tgaaaacagc ggccgcatcg ccaccactgc cgacggcacc gaagcttcac cgacttatct
     9241 ctccatcgaa accaccgaaa aaggagcggc aggcacattt atctccaatg gtggtcggat
     9301 cgagagcaaa ggcttattgg ttattgagac gggagaagat atcagcttgc gtaacggagc
     9361 cgtggtgcag aataacggca gtcgcccagc taccacggta ttaaatgctg gtcataattt
     9421 ggtgattgag agtaaaacta atgtgaacaa tgccaaaggc tcggctaatc tgtcggccgg
     9481 cggtcgtacc gtcatcaagg aggccagtat tcagactggc actaccgtat acagttccag
     9541 caaaggcaac gccgaattag gcaataacac acgcattacc ggggcagatg ttaccgtatt
     9601 atccaacggc accatcagca gttccgccgt aatagatgcc aaagacaccg cacacatcga
     9661 agcaggcaaa ccgctttctt tggaagcttc aacagttacc tccgatatcc gcttaaacgg
     9721 aggcagtatc aagagcggca agcagcttgc tttactggca gacgataaca ttactgccaa
     9781 aactaccaat ctgaatactc ccggcaatct gtatgttcat acaggtaaag atctgaattt
     9841 gaatgttgat aaagatttgt ctgccgccag catccatttg aaatcggata acgctgccca
     9901 tattaccggc accagtaaaa ccctcactgc ctcaaaagac atgggtgtgg aggcaggctc
     9961 gctgaatgtt accaatacca atctgcgtac caactcgggt aatctgcaca ttcaggcagc
    10021 caaaggcaat attcagcttc gcaataccaa gctgaacgca gccaaggctc tcgaaaccac
    10081 cgcattgcag ggcaatatcg tttcagacgg ccttcatgct gtttctgcag acggtcatgt
    10141 atccttattg gccaacggta atgccgactt taccggtcac aataccctga cagccaaggc
    10201 cgatgtcaat gcaggatcgg ttggtaaagg ccgtctgaaa gcagacaata ccaatatcac
    10261 ttcatcttca ggagatatta cgttggttgc cggcaacggt attcagcttg gtgacggaaa
    10321 acaacgcaat tcaatcaacg gaaaacacat cagcatcaaa aacaacggtg gtaatgccga
    10381 cttaaaaaac cttaacgtcc atgccaaaag cggggcattg aacattcatt ccgaccgggc
    10441 attgagcata gaaaatacca agctggagtc tacccataat acgcatctta atgcacaaca
    10501 cgagcgggta acgctcaacc aagtagatgc ctacgcacac cgtcatctaa gcattaccgg
    10561 cagccagatt tggcaaaacg acaaactgcc ttctgccaac aagctggtgg ctaacggtgt
    10621 attggcactc aatgcgcgct attcccaaat tgccgacaac accacgctga gagcgggtgc
    10681 aatcaacctt actgccggta ccgccctagt caagcgcggc aacatcaatt ggagtaccgt
    10741 ttcgaccaaa actttggaag ataatgccga attaaaacca ttggccggac ggctgaatat
    10801 tgaagcaggt agcggcacat taaccatcga acctgccaac cgcatcagtg cgcataccga
    10861 cctgagcatc aaaacaggcg gaaaattgct gttgtctgca aaaggaggaa atgcaggtgc
    10921 gcctagtgct caagtttcct cattggaagc aaaaggcaat atccgtctgg ttacaggaga
    10981 aacagattta agaggttcta aaattacagc cggtaaaaac ttggttgtcg ccaccaccaa
    11041 aggcaagttg aatatcgaag ccgtaaacaa ctcattcagc aattattttc ctacacaaaa
    11101 agcggctgaa ctcaaccaaa aatccaaaga attggaacag cagattgcgc agttgaaaaa
    11161 aagctcgcct aaaagcaagc tgattccaac cctgcaagaa gaacgcgacc gtctcgcttt
    11221 ctatattcaa gccatcaaca aggaagttaa aggtaaaaaa cccaaaggca aagaatacct
    11281 gcaagccaag ctttctgcac aaaatattga cttgatttcc gcacaaggca tcgaaatcag
    11341 cggttccgat attaccgctt ccaaaaaact gaaccttcac gccgcaggcg tattgccaaa
    11401 ggcagcagat tcagaggcgg ctgctattct gattgacggc ataaccgacc aatatgaaat
    11461 tggcaagccc acctacaaga gtcactacga caaagctgct ctgaacaagc cttcacgttt
    11521 gaccggacgt acgggggtaa gtattcatgc agctgcggca ctcgatgatg cacgtattat
    11581 tatcggtgca tccgaaatca aagctccctc aggcagcata gacatcaaag cccatagtga
    11641 tattgtactg gaggctggac aaaacgatgc ctataccttc ttaaaaacca aaggtaaaag
    11701 cggcaaaatc atcagaaaaa ccaagtttac cagcacccgc gaccacctga ttatgccagc
    11761 ccccgtcgag ctgaccgcca acggtatcac gcttcaggca ggcggcaaca tcgaagctaa
    11821 taccacccgc ttcaatgccc ctgcaggtaa agttaccctg gttgcgggtg aagagctgca
    11881 actgctggca gaagaaggca tccacaagca cgagttggat gtccaaaaaa gccgccgctt
    11941 tatcggcatc aaggtaggta agagcaatta cagtaaaaac gaactgaacg aaaccaaatt
    12001 gcctgtccgc gtcgtcgccc aaactgcagc cacccgttca ggctgggata ccgtgctcga
    12061 aggtaccgaa ttcaaaacca cgctggccgg tgccgacatt caggcaggtg taggcgaaaa
    12121 agcccgtgtc gatgcgaaaa ttatcctcaa aggcattgtg aaccgtatcc agtcggaaga
    12181 aaaattagaa accaactcaa ccgtatggca gaaacaggcc ggacgcggca gcactatcga
    12241 aacgctaaaa ctgcccagct tcgaaagccc tactccgccc aaattgtccg cacccggcgg
    12301 ctatatcgtc gacattccga aaggcaatct gaaaaccgaa atcgaaaagc tggccaaaca
    12361 gcccgagtat gcctatctga aacagctcca agtagcgaaa aacgtcaact ggaaccaggt
    12421 acagctggcg tacgacaaat gggactataa acaggaaggc ttgaccggag ccggagccgc
    12481 aattatcgca ctggccgtta ccgtggtcac ctcaggcgca ggaaccggag ccgtattggg
    12541 attaaacggt gcggccgccg ccgcaaccga tgcagcattc gcctctttgg ccagccaggc
    12601 ttccgtatcg ttcatcaaca acaaaggcga tgtcggcaaa accctgaaag agctgggcag
    12661 aagcagcacg gtgaaaaatc tggtggttgc cgccgctacc gcaggcgtag ccgacaaaat
    12721 cggcgcttcg gcactgaaca atgtcagcga taagcagtgg atcaacaacc tgaccgtcaa
    12781 cctagccaat gcgggcagtg ccgcactgat taataccgct gtcaacggcg gcagcctgaa
    12841 agacaatctg gaagcgaata tccttgcggc tttggtcaat accgcgcatg gagaagcagc
    12901 cagtaaaatc aaacagttgg atcagcacta catagtccac aagattgccc atgccatagc
    12961 gggctgtgcg gcagcggcgg cgaataaggg taagtgtcag gatggtgcga tcggtgcggc
    13021 tgtgggcgag atagtcgggg aggctttgac aaacggcaaa aatcctgcca ctttgacagc
    13081 taaagaacgc gaacagattt tggcatacag caaactggtt gccggtacgg taagcggtgt
    13141 ggtcggcggc gatgtgaata cagcggcgaa tgcggctaaa gtcgcgattg aaaataacct
    13201 attatctcaa gaagagtatg ctcttagaga aaaattgatc aaaaaagcca aagggaaagg
    13261 cctattatct ttagattggg gcagcctgac cgaacaagag gcaaggcagt ttatctattt
    13321 gattgagaaa gatcgatatt ctaatcaatt gcttgaccga tatcaaaaaa atccaagtag
    13381 tttaaataat caagaaaaaa atattcttgc atattttatt aaccaaacct ctggaggtaa
    13441 cacagcttgg gcagcttcga tactgaaaac gccccagtca atgggtaatc tcactattcc
    13501 ttccaaagat attaataaca ccttatcgaa agcctatcaa acattgagtc gttatgattc
    13561 ttttgattac aaatcagctg ttgccgcaca acctgcactt tacttattaa acggaccgct
    13621 tggcttcagt gtcaaagcag ctactgtggc agcaggagga tataacattg gacagggagc
    13681 gaaagcaatc tctaatggag aatatctgca tggtacagtt caggttgtta atggcacatt
    13741 gatggttgca ggatctgtat ctgcacaggc tgcaatatcg gccaagcctg cacctgttac
    13801 ccgttatctg agcaatgaca gtgctcctgc tttaagacaa gctttaactg ctgaaagcca
    13861 gagaatccgc atgaaactgc cggaagagta tcgacaaata gggaatcttg cgatagcaaa
    13921 aattgatgtt aaaggattac cgcaaaggat ggaagcattt agttctttcc aaaaagggga
    13981 acatggattt atttcgttac ctgaaacaaa aatttttaaa cctatatctg ttgataaata
    14041 tcataatatt gcctctcctc ctagaggaac attaagaaat atagatggag aatataaatt
    14101 acttgaaact atagcacagc aactcggaaa taatcgtaat gtatcaggta gaattgatct
    14161 atttacagaa ttaaaggcct gtcaatcttg cagcaatgtt attttagagt ttagaaatcg
    14221 ctatccaaat attcaattaa atatttttac aggaaaatag aatcatggaa aaaataaaaa
    14281 attatataga attcaagaaa ttatttaaaa atataacgga aaaatttgtc agagatttag
    14341 atataacatt aattgaagca gctgaatatt ctataattga attcgaaatt tatttatctg
    14401 aatatccgga agatagtaca tttattttta cagcatgggc aatctatctg ttagaaaata
    14461 actgtctcaa tgaaagagga aaagataatt ttttcatcgc ggaaatgaaa aaatgttatc
    14521 aacaaactaa tttggataga ataaaatcta tctattcttc gagtgactat catatttttg
    14581 ctaaggatgt aaacaagatt caacaaacat ttaaattaaa tcttgccaat atcatgcagc
    14641 aagagtagca actgtatttt taaccccgtc gggcaaaatt accaaaaacg gtaaatcact
    14701 aaaaatactt ttataccgcc gcagcagcaa gctgtagcct gcatgaaacc taaaatccat
    14761 gcgtaaggtg tgtgctccag cacgcacgcg ttccatgatt tacggctcaa tgccgtctga
    14821 aaagctcaca atttttcaga cggcatttgt tatgcaagta aatattcaga ttccctatat
    14881 actgcccaga tgcgtgcgtg ctgaagacac cccctatgct tgctgcagaa ctttcgggta
    14941 aaaccggtgt gagcattagc gcaccgtatg ccaatgagaa cagccgcatc attttgagtg
    15001 ccgccgatat agccgcagaa aacggcaaaa tcaaactgca atcctacggc gaccaatcct
    15061 attacgccgg acagggcgaa ctctatacct tagaacgccg cagctacaaa accggcaaat
    15121 ggtacaaccg caaacacatt accgaagtca aagaacacaa aaacgccaag cccgacgcag
    15181 taaccctcag cgcatcccaa ggcatcgaca tcaaatctgg tggcagcatc gacgcctacg
    15241 ccaccgcatt cgatgccccc aaaggcagca ttaacatcga agccgggcgg aaattgacac
    15301 tctatgccgt agaagagctc aactacgaca aactagacag ccaaaaaagg cgcagatttc
    15361 tcggcatcag ctacagcaaa gcacacgaca ccaccaccca agtcatgaaa accgcgctgc
    15421 cctcaagggt agttgcagaa tcagccaacc tccaatcggg ctgggatacc aaactgcaag
    15481 gcacacagtt tgaaaccaca ctgggtggcg caaccatacg cgcaggcgta ggtgagcagg
    15541 cacgggcaga tgccaagatt atcctcgaag gcatcaaaac cacgatccac aacgaaaccg
    15601 tgagcagcag caaatctgct ctatggcaaa aacaggcagg acggggcagt aacatcgaaa
    15661 ccttgcaatt gccgagtttc accggtcccg ttgcgcccgt actgtccgca cccggcggtt
    15721 acattgtcga tattcccaaa ggcaatctga aaaccgaaat cgaaaagctg gccaaacagc
    15781 ccgaatacgc ctacctgaaa cagcttcaga cggccaagaa cgtcgattgg aaacaggtgc
    15841 agctggccta cgacaaatgg gactataaac aggaaggctt gaccggagcc ggtgcagcga
    15901 ttgtggtgat tattgtaacc gctctaactt atggatacgg agcggctgca gcgggtagcg
    15961 taactgccgc aggaagtagt acagccgcag ctgcaacaac gacagcggca gcaactacca
    16021 ctgtttctac tgcagctgcc atgcaaaccg cagctttagc ctccttgtat agccaagcag
    16081 ctgtagccat catcaataat aaaggcgatg taggcaaagc attaaaagat ctcggcacca
    16141 gtgatacggt caagcagatt gttacttccg cactgacggc gggtgcatta aaccagatgg
    16201 gcgcagatat tgcccaattg aacagcaagg taagaaccga actgttcagc agtacgggca
    16261 atcaaaccat tgccaacctt ggaggcagat tggccaccaa cctcagcaat gcaggtatct
    16321 cagctggtat caataccgcc gttaacggcg gcagcttgaa agacaactta ggcaatgccg
    16381 cattgggagc attggttaat agcttccaag gggaagccgc cagcaaaatc aaaacaacct
    16441 tcagcgacga ttatgttgcc aaacagttcg cccacgcttt ggcgggttgt gttagcggac
    16501 tggtacaggg aaaatgtaaa gatggggcaa ttggcgcagc agttggggaa atcgtagctg
    16561 aatccatact tggcggcaga aaccctgcta cactcagcga tgcagaaaag cataaagtta
    16621 tcagttactc gaagattatt gcgggcagcg tggcggcact caacggcggc gatgtgaata
    16681 ctgcggcgaa tgcggctgag gtagctgtgg tgaataatgc tttgaatttt gacagtaccc
    16741 ctaccaatgc gaaaaagcat caaccgcaga agcccgacaa aaccgcactg gaaaaaatca
    16801 tccaaagtat tatgcctgca catgcggcag gtgcgatgac taatccgcag gataaggatg
    16861 ctgccatttg gataagcaat atccgtaatg gcatcacagg cccgattgtg attaccagct
    16921 atggggttta tgctgcaggt tggacagctc cgctgatcgg tacagctggt aaagcagcta
    16981 tcagcacctg tatggctaat ccttctggtt gtactgtcat ggttacgcag gctgctgaag
    17041 cgggcgcggg aatcgccacg ggtgcggtaa cggtaggcaa cgcttgggaa gctccagtag
    17101 gagcgttgtc gaaagcgaag gcggctaagc aggctatacc aacccagaca gttaaagaac
    17161 ttgatggctt actacaagaa tcaaaaaata taggtgctgt aaatacacga attaatatag
    17221 cgaatagtac tactcgatat acaccaatga gacaaacggg acaaccggta tctgctggct
    17281 ttgagcatgt tcttgagggg cacttccata ggcctattgc gaataaccgt tcagttttta
    17341 ccatctcccc aaatgaattg aaggttatac ttcaaagtaa taaagtagtt tcttctcccg
    17401 tatcgatgac tcctgatggc caatatatgc ggactgtcga tgtaggaaaa gttattggta
    17461 ctacttctat taaagaaggt ggacaaccca caactacaat taaagtattt acagataagt
    17521 caggaaattt gattactaca tacccagtaa aaggaaacta actaaatatg agtaactttg
    17581 aaaaaaaata tattttagaa ttaaatgatg ctttaagcca tttaaatcat aactctacct
    17641 catttgattt attgaaagtt ttgatttcat ggttatcaaa cgatattgtc attgataaat
    17701 ttaaaatttt aggttatgac tttagtaaat atatcgaaat gaatcccgat gactatccgg
    17761 ttgaaaaatc tatattgaat agagaggaaa ttatttatct caaaaacaat atttatcgta
    17821 aaatatcatc aggaaatttt aaatttcaat actttgtaca atatattaga gatattttag
    17881 aatatttatt tattgaacat attgaaagag tctgtcctta ctgcgaatgg ggtgaaatgc
    17941 aaaaattaga agaacaaaat acgcatgaaa cggtgtatct ctgtactcaa tgtggatgtg
    18001 ctttttataa cgataattca caatttttat taaaaacccc tttaaccatt ccaatgaaac
    18061 gtgatgaatt taaataaaca agccgtagcc tgcatgaacc ctaaaatcca cgtgtagcgt
    18121 gtgtgcgcca gcacgcatgc gttccatgat ttacggctca atgccgtctg aaaagctcac
    18181 aatttttcag acggcatttg ttatgcaagt aaatattcag attccctgta tgctgtacag
    18241 acgcgggagt gttaagcccc ccttgtttga agctccgcgg ctcctgccga gcttcaccga
    18301 ccccgttgtg cccaagctct ctgctcccgg cggctacatt gtcgacatcc ccaaaggcaa
    18361 tctgaaaacc gaaatcgaaa agctggccaa acagcccgag tatgcctatc tgaaacagct
    18421 ccaagtagcg aaaaacgtca actggaacca ggtgcaactg gcttacgata aatgggacta
    18481 taagcaggaa ggcttaacca gagccggtgc agcgattatc gcgctggctg ttaccgtggt
    18541 tactgcgggc gcgggagtcg gagccgcact aggcttaaac ggcgcagccg cagcagcggc
    18601 cgatgccgcc tttgcctcac tcgcttctca ggcttccgta tcgctcatca acaataaagg
    18661 cgatgtcggc aaaaccctga aggaactggg cagaagccgc acggtaaaaa atctggttgt
    18721 agcggcggca acggcaggcg tatccaacaa actcggtgcc tcttcccttg ccacttggag
    18781 cgaaacccct tgggtaaaca acctcaacgt taacctggcc aatgcgggca gtgccgcgct
    18841 gatcaacacc gctgttaacg gcggcagcct gaaagacaat ctggaggcaa atatcctggc
    18901 ggcattggtg aataccgcgc atggggaggc tgcgagtaag attaagcaat tggatcagca
    18961 ctatatcgcc cacaaagttg cccatgccgt agcaggctgt gcagcagcga cggcgaataa
    19021 gggtaagtgt caggacggtg cgattggcgc agccgtgggt gagattgttg gggaagcttt
    19081 ggttaaaaat accgatttta gcgatatgac cccggaacaa ttagatctgg aagttaagaa
    19141 aattaccgcc tatgccaaac ttgcggcagg tacagttgca ggcgtaacgg gaggagatgt
    19201 caatactgct gcacaaaccg cacaaaacgc ggtagaaaat aatgcggtta aagctgttgt
    19261 aactgctgca aaagtggttt ataaggtagc cagaaaagga ttaaaaaacg ggaaaatcaa
    19321 cgttagagat ttaaaacaga cgttgaaaga cgaaggttat aatttagccg acaacctgac
    19381 caccttattc gacgaaacat tggattggaa cgatgccaaa gccgttattg atattgtcgt
    19441 cggaacagag ctgaatcgcg ctaataaagg ggaagcggca caaaaggtca aggaagtttt
    19501 agaaaaaaat cgtccttata tccctaataa aggtgctgta ccgaatatga gtacatacat
    19561 gaaaaataat ccttttggaa aacagctggc tcaaatttca gaaaagacaa cgcttccgac
    19621 gcagcaaggg cagtctgtct tcttggtaaa aagaaaccaa gggttattaa aaaccggtga
    19681 taggttttat ttagatggcc aacataaaaa tcatttagag gtttttgata aaaatgggaa
    19741 ctttaagttt gttctaaata tggatggttc gcttaaccaa atgaaaactg gggcagcaaa
    19801 aggtcgtaaa ttaaacttaa aataggaaat tttatggaaa cattgaatga tataaaaaaa
    19861 atcttgatta atgtggggct ttatcaaggg tttgatttga cagatccaaa agtatcagaa
    19921 gaagttaatc atgaaacagc taatatgaaa tggattaaag attatacttc agacgggaat
    19981 tgggataatg aatttaagga ggatttaaaa aactttttag attatatgga agtatgccaa
    20041 ttagccctaa acgataaaaa tttcaaaatt gccagtaatt ctttatttat ggctatgatt
    20101 tacgcaggta atctatctct tatatttgat tcaataaaaa ctgatatatc aacattattg
    20161 agtgctgagt ataaaaagaa tagtttttca tggccatctc ttgatgaata gaaagcaagt
    20221 tgtagcctgc atgaaatcta aaacccatgc ataaggtgtg ggcttcagta tacgcgttcc
    20281 atgatttacg gccatatgcc gtctgaaaag ctcaattttt tcagacggca tttgttatga
    20341 aagtaaatat ttagattccc tgtatactgt ttagactcgt gtgtgctgag taagctgtag
    20401 tctgcatgaa acctaaaact cgctcaaaat taagctaaga cattagcagg gcaagggcga
    20461 aaattgaatc ttaaataagg tgattcagat gaaaaatttt aatgtagtaa aagaaagttt
    20521 aagagagtta ggaattaaac aaggatttga tctttatgag aaagccacaa ctgaaaaatt
    20581 gaatagtgaa gatcctcttg acttacaatg gctttctaac tattcatctg attggaatga
    20641 tgaattagaa gaagactttg attctttttt tcagcatatg aaggaatatc aatatgctat
    20701 tgacaatgaa gacattaaat ctgcatgtag ttcactatgt gaagctatgc tctatgttgg
    20761 taatattaaa aatttttttg agtttctcaa aagcgatatg attagactgt tgagaggtga
    20821 aagtaaaaca acagactttc aatggccgca atttgatgaa tagcagcaag ctgtagcctg
    20881 catgaaacct aaaatccatg cgtaaggtgt gtgcttcagc acgcacgcgt tccatgattt
    20941 acggctcaat gccgtctgaa aagctcacaa tttttcagac ggcatttgtt atgcaagtaa
    21001 atattcagat tccctatata ctgcccagat gcgtgcgtgc tgaagacacc ccctacgctt
    21061 gctatttgaa acagctccaa gtcaccaaag acgtcaactg gaaccaggta caactggcgt
    21121 acgacaaatg ggactataaa caggaaggct taaccggagc cggagcagcg attattgcgc
    21181 tggctgttac cgtggttact gcgggcgcgg gagccggagc cgcactgggc ttaaacggcg
    21241 cggccgcagc ggcaaccgat gccgcattcg cctcgctggc cagccaggct tccgtatcgc
    21301 tcatcaacaa caaaggcaat atcggtaaca ccctgaaaga gctgggcaga agcagcacgg
    21361 tgaaaaatct ggtggttgcc gccgctaccg caggcgtagc cgacaaaatc ggcgcttcgg
    21421 cactgaacaa tgtcagcgat aagcagtgga tcaacaacct gaccgtcaac ctagccaatg
    21481 cgggcagtgc cgcactgatt aataccgctg tcaacggcgg cagcctgaaa gacaatctgg
    21541 aagcgaatat ccttgcggct ttggtgaata ctgcgcatgg agaggcagca agtaaaatca
    21601 aacagttgga tcagcactac attgcccata agattgccca tgccatagcg ggctgtgcgg
    21661 cagcggcggc gaataagggc aagtgtcaag atggtgcgat cggtgcggcg gtcggtgaaa
    21721 tccttggcga aaccctactg gacggcagag accctggcag cctgaatgtg aaggacaggg
    21781 caaaaatcat tgctaaggcg aagctggcag caggggcggt tgcggcgttg agtaaggggg
    21841 atgtgagtac ggcggcgaat gcggctgctg tggcggtaga gaataattct ttaaatgata
    21901 tacaggatcg tttgttgagt ggaaattatg ctttatgtat gagtgcagga ggagcagaaa
    21961 gcttttgtga gtcttatcga ccactgggct tgccacactt tgtaagtgtt tcaggagaaa
    22021 tgaaattacc taataaattc gggaatcgta tggttaatgg aaaattaatt attaacacta
    22081 gaaatggcaa tgtatatttc tctgtaggta aaatatggag tactgtaaaa tcaacaaaat
    22141 caaatataag tggggtatct gtcggttggg ttttaaatgt ttcccctaat gattatttaa
    22201 aagaagcatc tatgaatgat ttcagaaata gtaatcaaaa taaagcctat gcagaaatga
    22261 tttcccagac tttggtaggt gagagtgttg gtggtagtct ttgtctgaca agagcctgct
    22321 tttcggtaag ttcaacaata tctaaatcta aatctccttt taaagattca aaaattattg
    22381 gggaaatcgg tttgggaagt ggtgttgctg caggagtaga aaaaacaata tacataggta
    22441 acataaaaga tattgataaa tttattagtg caaacataaa aaaataggag ttagtatgaa
    22501 atatatgatt agttttctaa aaaaaacatt tgaattaatg agttgggtgt tagtcatact
    22561 aataattggg acattttatg actattatca aataaggcaa tatgctgaat tagaaaagaa
    22621 atctatatca aatatcttgc tatatgccca aaaagaaaaa tttcgcttag agagtaaaga
    22681 taaatacatg cgaggaggat atacaaaata taaatttatt ttttcagaat atagtaatac
    22741 tactttttta aatttcataa atgacctgaa aaaagataat tatttaccac ttgacggcta
    22801 tggacatggt tttctatgta gaaaaggaga gtctatatca atcaatatat accctgaaat
    22861 taataaattt attttagtat ggggataccc tgaaaatctt tgcgctgatt ctaattaagt
    22921 aagcaagaat aggttatagg gaaataaaat tcaaatgaga aaattgaata atcacgatgt
    22981 tcataaacgg tatcaagatc gtttagaaga ggatgtagag ttcactatca actatgagct
    23041 tcctttgagt tgtttgtggt caaccatcaa agacttttcc agcgattttg aggaaaaaac
    23101 tgaagcgttc tttattcttt tcaaagagct gctgcgcaga ggtcatctga aactgcaacg
    23161 cgacgggcaa attatcgggc atacgcccga agaatgggaa caaatattta gggaagtatg
    23221 gcctgaatat gaaatcgaac ccaatccact tcccggctat gccccatttg atattggaat
    23281 gtggcttacg gtcgaggctc ctgcctacgc cgtatggata gatcccgaag acggtagcga
    23341 atactgggcg ggataaaata ccaatgtttg gaataaatcc cgtctgaaaa acagcttttt
    23401 cagacaggat ttattccaat tatcggtgat atacagagtt ttgtacaagc acagaccgct
    23461 gccgatcacc tgtttgcttt gctgggtgtg gttccgggta tcggtgaatc gatacaggcc
    23521 tataaagtag cgaaagcggc aaaaaattta caaggcatga aaaaagcctt ggacaaggca
    23581 gcaaccgttg ccactgcaca gggctatgtc agtaaaacca aaatcaaaat cggtcaaact
    23641 gaattaaggg ttactgcagc aactgacaaa caattgctga aaaccattag cgaaggaagg
    23701 gacacgacag gtaaaatgac cgagcagtta tttgactctt tagctaaaca aaatggcttc
    23761 agagtgcttt cgggcggcaa atacggcgca aataacggtt ttgatcatgt atggcaggct
    23821 gccgatggta gtgtcgtttt gattgtagaa agtaagcaga ttaggaacgg tacggtacag
    23881 ctgaatccga atggtgcggg tggatatacg caaatgagtg aggattggat taaacaagtt
    23941 attactaact taccagataa tcatcccact aaaaacatcc ttcgagaagc agtacgttcg
    24001 ggtaaaatca aaactgcggt tactggagta gatcgtcaaa caggtaaagc agttattctt
    24061 cctgttaaag ttccttctaa aaccaatata aggagataac aatggggcac aatatgatga
    24121 ccacccaaaa atggtatgaa catattacag atgtgattat agacaccact caaagattta
    24181 atagagagaa aagcgctgat agcatagagt atattaatga aagaaaggga tctccaattg
    24241 gagcgatctg tgtcgtttct atagatgttt atgcggcatg ctcacatgct tatctgtttg
    24301 aacacaacct taaaaagttc aaacagtatg cttatgctta cagtaaactt gagatactgg
    24361 caagtatggg atggtctgac cccacgcctt tcttctttcc ctgtgacatg ctcaacattc
    24421 aaaatccgat gtttctgatg ctgatgagcg acagcccaca gctgcgcgag tttctggtgc
    24481 gcaatatcga caacatcgcc aacgatacag aagcctttat aaaccgctac gacctcaacc
    24541 gtcatatgat ttacaatact ctgctgatgg tagagggtaa gcagcttgat cggttgaaac
    24601 aacgtagcga gaaagtcttg gcgcatccca cccctagcaa atggctgcaa aagcggttgt
    24661 acgattaccg cttcttcctc gctttcgccg aacaggatgc cgaggcgatg aaggccgcct
    24721 tagagccgct tttcgataaa aaaaccgcgc gtatggctgc caaagaaaca ttgtcctatt
    24781 tcgatttcta cctgcagccg caaatcgtta cctacgccaa aatcgcatcc atgcacggtt
    24841 tcgatttggg catagatcaa gaaatcgcac cgagggattt gattgtttac gatccgctgc
    24901 cggcagacga atatcaagac atcttcgatt ttatgaaaca gtatgacttg tcttacccgt
    24961 atgaatatct gcaggattgg atagattact atacgttcaa aaccgataag ctggtatttg
    25021 gtaacgcgaa gcgagagtga gccgtaaaac tctgagctcc tgttttatag attacaactt
    25081 taggccgtct taaagctgaa agattttcga aagctataaa ttgaagccct tccatagtac
    25141 atagatctgt gttgtggcgg ggctttacca cgctgattgc cggagaagaa ctcaacctgc
    25201 tggcaaaaca aggcatgaga tctttgcaat aacatgagtt gagacctttg caaaaaagcc
    25261 cttccccgac atccgaaacc caaacacagg atttcggctg ttttcgtatc aaatacctcc
    25321 taattttacc caaatatccc cttaatcctc cccggatacc ccataataga gcatccgctc
    25381 cgccttttag gcggtagcga gcgtacttag cctgttggcg gctttcaaca g
//
LOCUS       DAWHRS010000009        24515 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWHRS010000009 DAWHRS010000000
VERSION     DAWHRS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.009"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            207..1049
                     /locus_tag="WIB82_000268"
     CDS             207..1049
                     /locus_tag="WIB82_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ3841634.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVAV"
     gene            1079..1543
                     /gene="nrdR"
                     /locus_tag="WIB82_000269"
     CDS             1079..1543
                     /gene="nrdR"
                     /locus_tag="WIB82_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ3841635.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            1575..2684
                     /gene="ribD"
                     /locus_tag="WIB82_000270"
     CDS             1575..2684
                     /gene="ribD"
                     /locus_tag="WIB82_000270"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ3841636.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALVRAGVSRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLFSLL
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            2879..4294
                     /locus_tag="WIB82_000271"
     CDS             2879..4294
                     /locus_tag="WIB82_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ3841637.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSSQLVSKLAILLLLTVG
                     LLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPIA
                     LSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRAI
                     EENAPPARLSATAEFAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPLF
                     CTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFWLF
                     FAFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAAYLAGCI
                     LRHRKDLHKLFHYLKKQGFPL"
     gene            4291..4875
                     /locus_tag="WIB82_000272"
     CDS             4291..4875
                     /locus_tag="WIB82_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3841638.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADRIVYAY
                     NALPPPRNFLSGKPSKAASCSARCRVWTPSAPCI"
     gene            4827..5363
                     /locus_tag="WIB82_000273"
     CDS             4827..5363
                     /locus_tag="WIB82_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3841639.1"
                     /translation="MLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDN
                     LKAQAERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYD
                     TPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRLHAHVA
                     ALCSDDEIYRTTMAAYTM"
     gene            5383..6552
                     /gene="pglH"
                     /locus_tag="WIB82_000274"
     CDS             5383..6552
                     /gene="pglH"
                     /locus_tag="WIB82_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HEZ3841640.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAAATSNGRLRVKLLDEPGYRKNVSLARVASHRVFVRNLARWLHSPQAAE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNVPGEAVYIGTDFAAIAPPPPRFRSKTTR
                     LFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLEKLKQYENRAIKFYGYL
                     PYSEMMSIAKGCDIAVNAIHSHAMQSVTNKLSDYMALQKPILNSQTNAEVLSLLNLLP
                     HENYRSGDVDSFVQAAKDILKRKNDPVQSDEIVRRFRRDISYRKIVNLIERLANE"
     gene            6545..7786
                     /locus_tag="WIB82_000275"
     CDS             6545..7786
                     /locus_tag="WIB82_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ3841641.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPPFTGKRKLAVVGAGGHGKVVADLA
                     AALGRYREIVFLDDRAQGSVNGFSVIGTTLLLENSLSPEQYDVAVAVGNNRIRRQIAE
                     KAAALGFALPVLVHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLNAFVHISPGAHLSGNTHIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     MTVAGNPAKPLPRKNPKTGTA"
     gene            7788..7904
                     /locus_tag="WIB82_000276"
                     /pseudo
     CDS             7788..7904
                     /locus_tag="WIB82_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8024..9199
                     /locus_tag="WIB82_000277"
     CDS             8024..9199
                     /locus_tag="WIB82_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ3841642.1"
                     /translation="MLNTFLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALANGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPTTKAVIVVHLAGMPAEMDGIMALAKEHNLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYNHEHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKRLPEWTARRRENAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTNDEIAFCKENIEAVLAEATR"
     gene            9247..11157
                     /gene="pglD"
                     /locus_tag="WIB82_000278"
     CDS             9247..11157
                     /gene="pglD"
                     /locus_tag="WIB82_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ3841643.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFTAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSLVSAVLFFLNTLI
                     FEEKLRLALPVVYFLLLLVSVTGSRMVIRGLLSDHQKKHMTPVIIYGAGRSGRQLLEA
                     VKQIREYSAVAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWHELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            11213..12505
                     /locus_tag="WIB82_000279"
     CDS             11213..12505
                     /locus_tag="WIB82_000279"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ3841644.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAEEHAVENIGNYSNPQGDAALIDALTTFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(12557..13393)
                     /locus_tag="WIB82_000280"
     CDS             complement(12557..13393)
                     /locus_tag="WIB82_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ3841645.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKKLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCKPVWTHFS
                     RH"
     gene            13402..13644
                     /locus_tag="WIB82_000281"
     CDS             13402..13644
                     /locus_tag="WIB82_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841646.1"
                     /translation="MCVVNGGQGVSILSGGVAAVISKNRCMANAKEKPLCGHSRAGGN
                     PDFDLLEMFKVNRYFKLPDSCFRGNGDMTVFNFNLL"
     gene            complement(13789..13992)
                     /locus_tag="WIB82_000282"
     CDS             complement(13789..13992)
                     /locus_tag="WIB82_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841647.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(13970..14203)
                     /locus_tag="WIB82_000283"
     CDS             complement(13970..14203)
                     /locus_tag="WIB82_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ3841648.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            14743..18177
                     /gene="dnaE"
                     /locus_tag="WIB82_000284"
     CDS             14743..18177
                     /gene="dnaE"
                     /locus_tag="WIB82_000284"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ3841649.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLVAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSSDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMEAEPQIQELIEVEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDLPRWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            18325..18474
                     /locus_tag="WIB82_000285"
     CDS             18325..18474
                     /locus_tag="WIB82_000285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841650.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(18611..19543)
                     /locus_tag="WIB82_000286"
     CDS             complement(18611..19543)
                     /locus_tag="WIB82_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ3841651.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEIPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMNNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFAAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLDGIDVCLNIGALERGSGCKRREERDMAGLSERMAAELDRHG
                     AAVFFREYPNADHGNVSFYSLTDCVEYLRKAWQR"
     gene            complement(19613..21727)
                     /locus_tag="WIB82_000287"
     CDS             complement(19613..21727)
                     /locus_tag="WIB82_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ3841652.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTEGLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVRKRPTKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRCETFYAAADWDINPDTVLGAGYLYQQRRLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRKADSNYTFAGSKLNNTG
                     QADVAGLGTDIKQKAFAVDASYSRPFALGNTANEFVIGADYNRLRSTNEQGRSTLSKS
                     VALDGFRALPYNGILQNARAGNKGFNHSVTEENLDETGLYAKTVFRPLEGLSLIAGGR
                     VGHHKIESGDGKTLHKASKTKFTSYAGAVYDIDGSNSLYASASQLYTPQTSIGTDGKL
                     LKPREGNQFEIGYKGSYMDDRLNTRVSFYRMKDKNAAAPLDSNNKKTRYAALGKRVME
                     GVETEISGAMTPKWQIHAGYSYLHSQIKTASNSRDEGIFLLMPKHSANLWTTYQVTSG
                     LTIGGGVNAMSGITSSAGIHAGGYATFDAMAAYRFTPKLKLQINADNIFNRHYYARVG
                     SESTFNIPGSERSLTANLRYSF"
     gene            21887..22546
                     /locus_tag="WIB82_000288"
     CDS             21887..22546
                     /locus_tag="WIB82_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3841653.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRTLLEMPSETAVADITRTYSAHYLNPNNRNMSLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLAQHY
                     A"
     gene            complement(22634..23602)
                     /gene="ispH"
                     /locus_tag="WIB82_000289"
                     /gene_synonym="lytB"
     CDS             complement(22634..23602)
                     /gene="ispH"
                     /locus_tag="WIB82_000289"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ3841654.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(23631..24128)
                     /locus_tag="WIB82_000290"
     CDS             complement(23631..24128)
                     /locus_tag="WIB82_000290"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ3841655.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
     gene            complement(24334..24438)
                     /locus_tag="WIB82_000291"
                     /pseudo
     CDS             complement(24334..24438)
                     /locus_tag="WIB82_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         5565 a         7447 c         6315 g         5188 t
ORIGIN      
        1 ctcaaaagtt acggaacgaa aaacaaccaa aaccggacaa gtcggattcc cgcctgcgcg
       61 ggaatgacgg aatcttaagt ttccgtcttt gttttctgtt ttctgttttc gagggaataa
      121 tggggaacaa gccgtatttc agacggcatt ttcagttcgg ggtataatcc gaatacttgc
      181 gaccatctga atcattggga caaaccatgt gtcaactgct gggcatgaac tgcaatacgc
      241 cgaccgatat tatgttttcc tttgaaggct tccgccgcag gggcggcatt accgaccacc
      301 atgccgacgg tttcggtatc ggctttttcg aaggcaaagg cgtgcgcctg ttccacgacg
      361 acaagccgag cgcaaattcc cccgtcgccg acctcgtgcg tgcctaccaa atcaaatcgg
      421 aaaacgtcat cgcacatatc cgcaaagcat cgcaaggaca aacctcgctg gcgaacaccc
      481 atccctttat gcgcgaaatg tggggcggct actggctgtt tgcccacaac ggacatttga
      541 ttgatttttt ccccgaacag ggcgaatttt tccaccccgt cggcacaacc gattccgaac
      601 gcgcgttctg ccacatcctc aaccgcctgc gcacccgctt tgccgcccgt cccgacgacg
      661 acacgctgtt tgacgcgatt gcggggctga cgcacgaaat ccgcaagttc gggctgttta
      721 actttatgct ttcagacggc attgccctgt ttgcccacgc cagcacgctg ctgcactaca
      781 tcgtccgcca agccccgttc ggcaaggcgc gcctgctcga cgacgacgtg atggtcgatt
      841 ttgccgaagt aaccacgccc tccgaccgcg tcgccgttat cgccaccctg ccactgaccc
      901 gcgacgaatc atggtcccaa cttgccgtgg acgaactggt catgttccgc gaaggcaaca
      961 tcgtccgaca cgaccgtccc gaaaaccccg tctatatgag tgccgaagaa ggtctggaaa
     1021 tcgcccgcgc cgccggcgtc gccgtctgaa cttcagacga cataggagga cgaacccgat
     1081 gaaatgcccg ttttgcgccc accccgacac ccgcgttgcc gattcgcgtc tgatggaaga
     1141 acgcaacgcc gtgcgccgcc gccgccactg ccccaactgc ggcaaacgct tcggcacgct
     1201 cgaaaccgcc gaactcaaaa tgcccgccgt catcggtccg gacaaaaaac gctcgccctt
     1261 taatgcacaa cgcctccgca acgacctgac cgccgccgcc cgaaaatccg ccctgacacc
     1321 cgaacagatc gacgaaaccg tccgcctgac ggaacacagg ctctacactt cgggtcagcg
     1381 cgacatcccc tctgccgcac ttgccgacat ggtgctaaaa gaactgctcc gacaagatac
     1441 ggaagccgcc gtccgtttcg ccgccctgca caaacgcttc gacaatccgg cagactttgc
     1501 ctcgtggctg gcgcaagccg tcaaaacagg cggcaaagcc tgattccccc aacccatact
     1561 gatacggtat ccctatgttt tcggacacag atatatccat gatggaaaac gccctccgac
     1621 ttgccgcttt ggggcgtttt tccacttcgc ccaatccgcg cgtcggctgc gttatcgcac
     1681 acggcagcca aattgtcggg caaggcttcc acgtcaaagc gggcgaaccc catgccgaag
     1741 tccacgccct gcgtcaggcg ggcgaaatgg cacaaggcgc gaccgccttt gttaccctcg
     1801 aaccgtgcag ccattacggg cgcacaccgc cctgtgccga agcactggtg cgggcgggcg
     1861 tgtcccgcgt cgttgccgcc atgcgcgacc ccaacccgct ggttgcaggc aaagggcttg
     1921 ccctgctcga agcagcaggc atcaagacgg aatgcggttt actcgaacat caggcaaggg
     1981 aactcaaccg aggcttcctg tcgcgcatcg aacgccgccg cccctttgtc cgcctcaaat
     2041 gcgccgtttc gctggacggc aaaaccgccc tttcagacgg cagcagcttt tggattaccg
     2101 gcgaagacgc gcgtgccgac gtacaggttt tgcgtgccga aagctgcgcg gtgctgaccg
     2161 gcatcggcac ggtgttggcg gacaatcccc ggctcaacgt ccgcgctttt ccaactttgc
     2221 gccaacccgc acgcatcgtt ttagacagcc gcctgcgcct gcccccgaac agccatttgg
     2281 ttaccgacgg acaatctccg acctacatcg ccacactcga acgcaacgaa gacagactgc
     2341 acccctatcg ggaacacgca cacgtccgca tcctgatgcc gtctgaaacg gcagacagca
     2401 aaatcgacct gcaccacctg atgcgcctcc ttgctgacga aggtttcggc gaaatcatgg
     2461 tcgaagcagg ctccgaactc acatccgcat ttttggcaga aaatctggca gacgaaatcg
     2521 tcctgtaccg ttcgcccaaa atcctcggca gcggcaaaga cctgttttcc ctgctcgaaa
     2581 accgcgccgc cctttccgca ccgcccttgt ggacacccgt ttcaagcgaa atcctcggac
     2641 acgacatcaa aaccgtgttc cgaaaaaacg gcaacgcctt ttaaagggtt tgcgccgttt
     2701 cactatataa taacgccgat aaaaaacggc cccgttcaga ccgccgcccg cccgaaaaaa
     2761 cgcaacccgg actgccgcac cccgccggca gccgcgacgg tctgaaagcc gtcaaattcc
     2821 gatcaagaaa ggcttcagac ggcacaggca gcatcccgcc gccgcccgga catcaaaaat
     2881 ggacacaaaa gaaatcctcg gctacgcggc aggctcgatc ggcagcgcgg ttttagccgt
     2941 catcatcctg ccgctgctgt cgtggtattt ccccgccgac gacatcgggc gcatcgtgct
     3001 gatgcagacg gcggcggggc tgacggtgtc ggtgttgtgc ctcgggctgg atcaggcata
     3061 cgtccgcgaa tactatgccg ccgccgacaa agacactttg ttcaaaaccc tgttcctgcc
     3121 gccgctgctg tctgccgccg cgatagccgc cctgctgctt tcccgcccgt ccctgccgtc
     3181 tgaaatcctg ttttcgctcg acgatgccgc cgccggcatc gggctggtgc tgtttgaact
     3241 gagcttcctg cccatccgct ttctcttact ggttttgcgt atggaaggac gcgcccttgc
     3301 cttttcgtcc tcgcaactcg tgtccaagct cgccatcctg ctgctgctga cggtcgggct
     3361 gctgcacttt ccggcgaaca ccgccgtcct gaccgccgtt tacgcgctgg caaaccttgc
     3421 cgccgccgcc tttttgctgt ttcaaaaccg atgccgtctg aaggccgtcc ggcgcgcacc
     3481 gttttcatcc gccgtcctgc atcgcggcct gcgctacggc ataccgatcg cactaagcag
     3541 catcgcctat tgggggctgg catccgccga ccgtttgttc ctgaaaaaat atgccggcct
     3601 agaacagctc ggcgtttatt cgatgggtat ttcgttcggc ggggcggcat tattgttcca
     3661 aagcatcttt tcaacggtct ggacaccgta tattttccgc gcaatcgaag aaaacgcccc
     3721 gcccgcccgt ctctcagcaa cggcagaatt cgccgccgcc ctgcttgcct ccgccctctg
     3781 cctgaccggc attttctcgc ccctcgcctc cctcctgctg ccggaaaact acgccgccgt
     3841 ccggtttatc gtcgtatcgt gtatgctgcc tccgctgttt tgcacgctgg tagaaatcag
     3901 cggcatcggt ttgaacgtcg tccgaaaaac acgcccgatc gcgctcgcca ccttgggcgc
     3961 gctggcggca aacctgctgc tgctggggct tgccgtaccg tccggcggcg cgcgcggcgc
     4021 ggcggttgcc tgtgccacct cattctggtt gttttttgcc ttcaagaccg aaagctcctg
     4081 ccgcctgtgg cagccgctca aacgcctgcc gctttatacg cacacattgc tctgcctgac
     4141 ctcctcggcg gcctacacct gcttcggcac accggcaaac taccccctgt ttgccggcgt
     4201 atgggcggca tatctggcag gctgcatcct gcgccaccgg aaagatttgc acaaactgtt
     4261 tcattatttg aaaaaacaag gtttcccatt atgaaaatcg ttttgaccac atctatggca
     4321 ggcttgggcg gcacggaaac cgccaccgtc cgcctcggca ggctactcag gcagcacgga
     4381 cacgacatca tgcttgcctc ttcggacgga ccgttgctca aagaggcgca agccttgggc
     4441 atacgttggc aacccgtcga tttctatcgc ggcggccttt tcggctacct caaaggtatg
     4501 attgcctacg cccgaatgct gcggcgcgaa caacccgaca tcatcgactg ccaaatggcg
     4561 cgcgtcgtac ccgcctgcgc cattgccgcc aaaatcgttt cgccgaaaac caaaatcatc
     4621 taccactcgc acggtttgga cgcggcaacc taccccaaaa tcgccaaact cttcgacaag
     4681 ctgggcgtgt acatcatcgg taactgcaaa cacgaacgcg aaaaactcat ccgccacggt
     4741 ttccccgccg accggattgt ctacgcctac aacgcactcc cccccccccg gaatttcctt
     4801 tctggaaaac cgtcaaaggc tgcgtcatgc tcggcacgct gtcgcgtctg gacaccgtcc
     4861 gcgccgtgca tctgatgctg gacattttca aaaaaatggt cgaccgcgat atgcccgtac
     4921 gcctgaacgt ggcgggcata ggcgaagaaa tggacaattt gaaagcgcag gcggaacgtc
     4981 tgggcataga cggcaaagtt accttcctcg gcggcgtgcg cgatttgacc gcctacttca
     5041 aagacgtgga tattttggtg aacacgcccc actgcatcgg cgaccacggc gcaggtgtgg
     5101 gcaacaacat cttagaagcc ggactttacg acacacccgt cgttacctac gatatggcgg
     5161 gcatttccga aatggtcgtc aacggggaaa cgggttactg catccccttc ggcgacgaag
     5221 aagcctttat cgccgccgta gacacgctcg tcagacaacc gctgttgcgc gaagcaatgg
     5281 gcaaaaggct gcacgcacac gtcgccgcac tctgttccga cgacgaaatc taccgtacca
     5341 ccatggcggc ctataccatg taatgccgtc tgaaagccgc cgatgaacat caccatagtc
     5401 gccccttatt gctcgctgcc gtccgaaccg tatttcaacc gcttttggta tctggcagaa
     5461 cgattgtcgc aatcgcacga cgtgttgctg attaccagcc gtttccgcca ctatgacaaa
     5521 tcattcagac ggcatgaaga tgctgccgcc acttcaaacg gcaggctgcg cgtcaagctg
     5581 ctggacgaac ctggctaccg aaaaaacgtc tccctcgccc gcgtcgccag ccaccgcgta
     5641 ttcgtccgca atttggcacg ctggctgcac agcccgcagg cggcggaaca ggacatcgtc
     5701 tattctgcct atccgctgat ggcgaccaac ctgctgttgg gcaaacacaa agcgcgtttg
     5761 ggctacaaac tgattgtcga tgtgcaggac gtatggccgg agtctttctc ctcggtcgta
     5821 ccatttttga aaaaagtacc gcacaagctg ctgccctttg cttcacgcgc caaccgtgcc
     5881 taccgctgcg ccgacgcgct ggttgccgta tcgcagactt atctcgaccg cgccaaagaa
     5941 gccaatccga acgtacccgg cgaagccgtc tatatcggta cggattttgc cgccatcgcc
     6001 cccccccccc cgcgattccg ctccaaaacc acccgccttt tctacttggg aacgctcagt
     6061 tacagctacg acgtggaaac cgtgtgcaaa ggtgttcgga aacttttaga cgacggcgaa
     6121 aacgtcgaat tgcacattat gggcggagga cccgatttgg agaaactcaa acaatatgaa
     6181 aatcgcgcca tcaagtttta cggttattta ccctattctg aaatgatgtc gattgccaaa
     6241 ggctgcgaca ttgccgtcaa cgccatccat agccacgcca tgcagtcggt taccaacaaa
     6301 ctctccgact atatggcttt gcaaaaaccg attctgaaca gccagaccaa tgccgaagtc
     6361 cttagcctct tgaacctgct gccgcacgaa aactaccgtt ccggcgacgt ggacagcttc
     6421 gttcaagccg ccaaagatat tttgaaacgc aaaaacgacc ccgttcagtc cgatgaaatc
     6481 gtccgccgct tcaggcgcga catttcctat cggaaaatcg tcaacctgat tgaaagattg
     6541 gcaaatgagt aaagccgtca aacgcctgtt cgacatcatc gcatccgcat cggggctgat
     6601 tgtcctctca cccgttttcc tgattttggc gtacctcatc cgcaagaatc tgggttcgcc
     6661 cgtcttcttc attcaagaac gccccggaaa ggacggaaaa ccttttaaaa tggtcaaatt
     6721 ccgttccatg cgcgacgcgc ttgattcaga cggcattccg ctgcccgaca gcgagcggct
     6781 cacacccttc ggcaaaaaac tgcgtgccgc tagtttggac gaactgcctg aattatggaa
     6841 tgtcttaaaa ggcgacatga gcctggtcgg cccgcgcccg ctgctgatgc aatatctgcc
     6901 gctgtacgac aacttccaaa accgccgcca cgaaatgaaa cccggcatta ccggctgggc
     6961 gcaggtcaac gggcgcaacg cgctttcgtg ggacgaaaaa ttcgcctgcg atgtttggta
     7021 tatcgaccac ttcagcctgt gcctcgacat caaaatccta ctgctgacgg ttaaaaaagt
     7081 attaatcaag gaagggattt ccgcacaggg cgaagccacc atgccccctt tcacaggaaa
     7141 acgcaaactc gccgtcgtcg gtgcgggcgg acacggaaaa gtcgttgccg accttgccgc
     7201 cgcactcggc cggtacaggg aaatcgtttt tctggacgac cgcgcacaag gcagcgtcaa
     7261 cggcttttcc gtcatcggca cgacgctgct gcttgaaaac agtttatcgc ccgaacaata
     7321 cgacgtcgcc gtcgccgtcg gcaacaaccg catccgccgc caaatcgccg aaaaagccgc
     7381 cgcgctcggc ttcgccctgc ccgttctggt tcatccggac gcgaccgtct cgccttctgc
     7441 aacagtcgga caaggcagcg tcgttatggc gaaagccgtc gtacaggcag gcagcgtatt
     7501 gaaagacggc gtgattgtga acactgccgc caccgtcgat cacgactgcc tgcttaacgc
     7561 tttcgtccac atcagcccag gcgcgcacct gtcgggcaac acgcatatcg gcgaagaaag
     7621 ctggataggc acgggcgcgt gcagccgcca gcagatccgt atcggcagcc gcgcaaccat
     7681 tggagcgggc gcagtcgtcg tacgcgacgt ttcagacggc atgaccgtcg cgggcaatcc
     7741 ggcaaagccg ctgccgcgca aaaaccccaa gaccgggacg gcataagcga ttaaaatata
     7801 gtggattaaa tttaaatcag gacaaggcga cgaagccgca gacagtacaa atagtacggc
     7861 aaggcgaggc aacgccgtac tggtttaaat ttaatccact atacaccccc gtacagaccg
     7921 attttgacaa cacctgcggc gcgcgcccga ttcttcggaa cacgcccccc ttcagacggc
     7981 atagggtcgg aaatgccgtc tgaaaaccga cggacaaacc atcatgctga acactttcct
     8041 ttccccgtgg ccctgcttca cccaagaaga agccgatgcc gtttccaaag tcctgctgtc
     8101 caacaaagtc aactactgga cgggcaacga atgccgcgaa tttgaaaaag aatttgccgc
     8161 ctttgccggc acgcggtacg ccgtcgccct tgccaacggc acgctggcac tcgatgtcgc
     8221 gctcaaagca atgggcatag gcgcgggcga cgatgtgatt gttacctcgc gcaccttcct
     8281 cgcttccgcg tcctgcattg tgaacgcggg cgcaaacccc gtgtttgccg atgtggattt
     8341 gaacagccaa aacatcagcg cggaaaccgt caaagccgcg ctgacaccga ctaccaaagc
     8401 cgtcatcgtc gtccacctcg ccggtatgcc cgccgaaatg gacggcatta tggctttggc
     8461 aaaagaacat aatctttggg taatcgaaga ctgcgcccaa gcgcacggcg caaaatacaa
     8521 aggcaaatcc gtcggctcta tcggacacgt cggcgcgtgg tcgttctgcc aagacaaaat
     8581 catgaccacc ggcggcgaag gcggtatggt tacgaccaac gacaaaaccc tgtgggaaaa
     8641 aatgtggtcg tacaaagacc acggcaaaag ctacgatgcc gtgtacaacc acgaacacgc
     8701 gcccggtttc cgctggctgc acgaaagttt cggcacaaac tggcgtatga tggaaatgca
     8761 ggcggtcatc ggacgcatcc agctcaaacg cctgcccgaa tggacggcgc gccgccgaga
     8821 aaacgccgcc aagctggcgg aaagcctagg caaattcaaa agcatccgct tggttgaagt
     8881 cgccgactac atcggacacg cgcaatataa gttctacgcc ttcgtcaaac ccgaacacct
     8941 caaagacggc tggacgcgcg accgcatcgt gaacgaactg aacgcgcgca aagtcccctg
     9001 ctatcaaggt agctgctccg aagtgtattt agaaaaagcc ttcgacaaca cgccgtggcg
     9061 tccgaaagag cgtttgaaaa atgctgtcga gttgggcgac accagcctga tgtttttggt
     9121 gcacccgacg ctgaccaacg acgaaatcgc gttctgcaaa gaaaacatcg aagccgtatt
     9181 ggcagaagcc acacgataac ccttcagacg gcatatgccg cctgaaagca cataccgccc
     9241 cacgatatga ctctggaaac cctgcttgcc ctaccgcgca acatcaagaa aatctgtttc
     9301 ctcatacacg attttctgat gattttcacc gccttctggt ttacccaaag cctgaaggcc
     9361 gactactcga acgaatggtt cgaccctgcc aactggcagt ctttcctatt gaccgcgctg
     9421 ttgaccatcg gcctgttcgt cagaatgggg ctctaccgtg ccgttacacg ctttgtcagt
     9481 ttccacgtcc tgaccaccgc ctttgccggc agcctcgtct ccgccgtgtt gtttttcctc
     9541 aatacgctga tatttgaaga aaaattgcgc ctcgccctgc ccgtagtcta ctttctgctg
     9601 ctgcttgtct ccgtaaccgg atcccgcatg gtcatacgcg gccttttatc cgaccaccaa
     9661 aaaaaacata tgacccccgt catcatttac ggtgcgggac gatcgggcag acaactgctc
     9721 gaggccgtca aacagatacg cgaatattcc gcggtcgcct ttgtggacga caaccccaag
     9781 ctttggcaca ccgtcatcta cgaccttgcc gtttaccagc ccgatgccat cgccttcctc
     9841 atcgaacgtt acggcgtgga aaaaatcctg ctcgccattc ccagcgcgac tcaggaacaa
     9901 cgccgccgaa tcatcagcaa actggaagcc tatccgtgcg aagtgttgac cattcccgga
     9961 atgaaagacc tgatggacgg gaaaatcagc atcggcacgc tcaaaaaaat ctccgtgtcc
    10021 gacctgctcg ggcgcgattc cgtcgcaccc gacgaccgtc tgatgagtgc cgacatcgaa
    10081 ggcaaaaccg tcatggtaac cggtgcgggc ggctccatcg gttcggaact ctgccgccag
    10141 attatccgcc gccgccccga aaaactgctg ctgttcgagt tatccgaatt cgccctgtac
    10201 gccatcgaaa aagaattgcg cgaaacctgc atccaaaaac gtctcgatac cgaaatcctg
    10261 ccctttctcg gctcggtgca aaaccgcacg ctgctcgaac acgtcatgac cgccttttcc
    10321 gttgcgaccg tctatcacgc cgctgcctac aaacacgtcc ccatggtcga atttaacacc
    10381 gtcgaaggca tacgcaacaa catcttcggc acactcgagt gcgcgcttgc cgccacggca
    10441 tcgggcgtaa gaaccttcgt cctcatctcc accgacaaag cagtccgccc caccaacacc
    10501 atgggtgcca gcaaacgcat ggcggaactc tgccttcagg cgctcgccgc cgaacccgga
    10561 caaaaaaccc gcttcagcat ggtacgtttc ggcaatgttt taggttcgtc cggctccgtt
    10621 gtcccgctgt ttgaaaaaca gattgccgaa ggcggcccga ttaccctgac ccaccccgaa
    10681 atcacacgtt atttcatgac catacccgaa gccgcccaac tcgtcataca ggcaggcgcg
    10741 atgggtacgg gcggcgacgt attcgtcctc gacatgggcg aatccgtcaa aatcatcgac
    10801 cttgcccgcc aaatgattac cctaagcggc ctcaaaccca aaacacccga acaacccgac
    10861 ggcgacatcg aaatcctcat taccggactg cgtcccggag aaaaactcta cgaagagctg
    10921 ctcatcggcg acaacgtccg caaaaccggc catccgcgca tcatgaccgc caacgagacc
    10981 atgctgccgt ggcacgagct ctccgcccta ctcgaccgca tccgtgcggc ctgcgaccgt
    11041 tacgaccagc aggcaatccg caccctgctc atcaacgccc cgaccggctt tgccccgagc
    11101 gacggtatct gcgacctgct ttgggtacga gaaacacaca gaaaaaatgc cgtctgaacc
    11161 ttcagacggc ataacgtaca aaccaaccta ccttacacac acggagtttg acatgcagtt
    11221 ttcagcattc ggcgaaaaat tcacgcaaca cagcggcatc ctccaactga tggacgacct
    11281 cggcgacgcg ctcaaaagcg acaagcccgt caacatgctc ggcggcggca acccggcgcg
    11341 cattccggaa atcgatcggg cattcgccga catattctcc aaactggcgg aggaacacgc
    11401 cgtcgaaaac atcggcaact actccaatcc ccaaggcgat gccgcgctga ttgacgcgct
    11461 gaccaccttc ctcaaccgcg aatacggctg gaatctgacc gccgacaata tcgcgctgac
    11521 caacggttcg caaaacgcgt ttttctattt attcaacctc ttcggcggca aattcaacct
    11581 ttcagacggc acatccgcag aaaaagccat tttgttgccg ctcgcgcccg aatacatcgg
    11641 ctatgccgac gtgcatgtcg aagggcagca cttcgtttcc gtcaagccca aaatcgaaaa
    11701 cgtcgaacac gaaggcgaag caggcttctt caaataccgc gtggactttg acgcactgga
    11761 aaacctaccc gaactcaaag cgggcaaaat cggcgcgatt tgctgttcgc gcccgaccaa
    11821 cccgaccggc aatgtgttga ccgacggcga aatggcgcgt ttggacgctt tggcacgtga
    11881 acacgggatt ccgctgatta tcgacaacgc ctacggaatg ccgttcccca acatcattta
    11941 cagcgacgta acgctgaatt ggcacgaaaa catcatcctc tgcttcagcc tttccaaagt
    12001 cggcctgccg ggcgttcgca ccggcatcat cgtcgccgcg ccggaagtgg tcaaagccgt
    12061 cagcagcctg aacgcgattg tgaaccttgc ccccacgcgc ttcggcgcgg ccatcgccgc
    12121 cccgctactg gaaagcggcg agatgaaacg gcttgccgac caagtcatcc ggccgtttta
    12181 ccgcaatcag gcgcaaaccg ccgtctcgct gctcaagcgc gagctgggcg cgtacccgat
    12241 gaaaatccac aaacccgaag gcgcgatttt cctgtggctc tggtttgaaa acctgcccgt
    12301 ttcttcgcaa accctgtacg aaatgctcaa agccgaaggt acgctgatta ttccgggcga
    12361 acatttcttc gtcggcatcg acacgcagga ttacccgcac gcgggcgagt gcatccgcat
    12421 gagcatcgcg caggacgctc aaacgctgga aaaaggcatt gccgccatcg gcaaaaccgt
    12481 ccgaaaactg tacgacaacg tttaaaccgc aaaaaatgcc gtctgaaaag ttttcagacg
    12541 gcatttttat ccgcattcaa tgtcgggaaa agtgcgtcca caccggtttg cagttttccg
    12601 gcaattcggg acacgcgccg agaatgccgc cgctgaagtc gtttaaacgg tggaagtcgc
    12661 tgcccgcgct tgccaacaat ccgaagcgtt ctgccaaaag cgcgtagttg aggcggtcgt
    12721 ttttgcaaca gtttccgctg tggacttcga tacccgcacc gccgagtttt ttaaattctt
    12781 caaacagatt gcgcttggcg gtggcggaca aatcataacg catcggatgg gcgatgaccg
    12841 ccaacccgcc cgcgccgttg acggcggaga cgcagtcttc cagcgtcgcc cattcgtggc
    12901 ggacggcgca ggatttgccg tcgcccaagt atttggtgaa cgcctgctgc ttgtttttga
    12961 cgtgtcccac ccgaatgagg aactcggcga cgtgtgtgcg gctgaccatt tctttgtttg
    13021 ccgccagcgc gagcgcgccg tcgtatgcgc ccgaaatgcc gcgtttttcc agcttggcgg
    13081 cgatggcttc gaggcgtttc agacggcctt gccgcacttg cgccaacagg ttttgcaggt
    13141 tttcgtcttg ttcgtcaaag tctaagccga caacgtgtat ggtacgcccg cgccacgtta
    13201 cggagatttc cacgccgtta atcaggcgca aaccgagcct gtcggcttcg gcacgcgctt
    13261 cggcgatgcc gccggtgtgg tcgtggtcgg tcaacgccag cagcgtgcag ccgttttgat
    13321 gcgcgaggcg cacaacttcg gcgggggaga gcataccgtc ggaaacggtg gaatggcagt
    13381 gcaggtctat cataaggcgt tatgtgcgtg gtaaatggtg ggcagggtgt ttcaatattg
    13441 tcgggcggtg tggcggcggt gatttcaaaa aacaggtgta tggcaaatgc aaaggaaaag
    13501 ccgctatgcg gtcattcccg cgcaggcggg aatccagact ttgatttatt ggaaatgttt
    13561 aaggttaacc gctatttcaa acttccggat tcctgctttc gagggaatgg cgatatgacc
    13621 gttttcaatt ttaatctact ataaaagact gtctgaaaac gtggttttat agtgaattaa
    13681 atttaaaccg gtacagcgtt ggctcgcctt gccgtactat ttgtactgtc tgcggctcgc
    13741 cgccttgtcc tgatttttgt taattcacta tatcaagccg aaccgttttc agacggcatc
    13801 gtccgaccaa cccgcttctt tcaatttccg ccgttgcgcg tcgtagcggg ccttatccgc
    13861 ccaataaatc gtctgaatac acgcctcgcc gcagtcgctg ccgcagcatt cccacgactc
    13921 gggtcggacg ggttcgtcta aaagcggctc gcccaaaagg gcttcggctt tatgcttcag
    13981 ggtcatatcc atcggcgatt tccaagcgcg caccgtcaaa ctcgatgact tcgccgccgc
    14041 gtattttggc ggttttacgg gtttcggttt cgccgttgcg cgacaccaac ccttcggcaa
    14101 tcaacgcctt cgcctgtccg ccgctttcgg caagtccggc caatttcaag aggtcgcaca
    14161 gggcgatgta ttcgttgtct tcgagataga cggtggcttc cataatgttc ccttgcagaa
    14221 agaggccgtt attgtagcac ctgccgccgc cgtacccaaa attaccgcaa aacaggcagc
    14281 acatcccgcc ccgcctgttc cgtaaaaatg ccgtctgaaa ccccatatgc cgtcatccga
    14341 tcaaagaaat cctgcccaac agcaggctgc aaatcctgtt ccccgacgaa tccgcattga
    14401 cgctgatgca catcctcaaa cacgaactgc ccgatacact ggtcatcggc atcagccatc
    14461 aaaacgaaat caaaaacctg tttgaacgta aaattaattt aactgcttgc catccgtccg
    14521 aataaggtat atagttattt ataactagtt tgatagttct ttatatctat caatactcct
    14581 tgggaagcct ccaccacacg gtaggaggca tttttttgcc gtcgtaaaag ctcaaaaaca
    14641 tttgcaggtc atgccgtctg aatctgaaac ggcatgacct acaccgccat ctaaagacaa
    14701 ccctgctaca atacgccttt tattgttcac gccgattttg ccatgaccga gccgacctac
    14761 atccccttgc gcctgcatac cgagttctca attaccgacg gtatggtgcg gattaaaaaa
    14821 ctggttgcca aagcgcagga atacggtttg cctgctttgg gcatcagcga tttgatgaac
    14881 gaattcggct tggtgaaatt ctataaagcc tgccgcggcg cagggattaa acccatcggc
    14941 gcggcggacg tgtggatagg caatccgaat gcgcccgaca agccgtttcg cgccatgctg
    15001 attatccgca acgatgcggg ctatctgcgc ttgagcgagc ttctgacggc ggcttatgtc
    15061 ggcaaagacc gcaatatcca tcatgcggaa ctcaatcccg aatggctgga aaacggcgac
    15121 aacagcggct tgatttgttt gagcggcgca cattacggcg aagtgggcgt gaatctgttg
    15181 aacggcaatg aagacgcggc gcgtgcggcg gcgttgaaat atgcggcatg gtttcccgat
    15241 gcgttttatt tagagctgca acgcctgccc gaacgccccg aatgggaggc ttgcgtttcc
    15301 ggcagcgtga agctggcgga ggaactgggt ttgccggtgg tggcgaccca cccgacgcag
    15361 tttatgagca gcgatgattt caacgcgcac gaggcacggg tgtgtattgc gggcggctgg
    15421 gtgctgacgg acaaaaaacg tccgcgcgat ttcacaccgg gccagttttt cattccgccg
    15481 gaaacgatgc tggagcgttt cgccgatttg cccgaggcct tggaaaacac ggtagaaatt
    15541 gccaaacgct gcaatctgca catcacattg ggcaaaaact tcctgcccct tttccccacg
    15601 cccgacggtt tatcgcgcga tgactatctc atcaaactct caaacaaggg cttgcaggaa
    15661 cgcatggttc agctttatcc cgacgaggcg gagcgtgcgg caaaaatgcc ggaatatcag
    15721 gaacgtttgg attttgagct gaacatcatc atccagatga aattccccgg ctatttcctt
    15781 atcgtacaag actttatcaa ctgggcgaaa acgcacggct gtccggtcgg gccgggacgc
    15841 ggttcgggcg cgggttcgct ggtggcgtat tcgctgaaaa ttaccgacct cgacccgctg
    15901 aaatacgcgc tgctgtttga acgtttctta aaccccgaac gcgtctccat gcccgacttc
    15961 gacgttgact tctgtcaggc aaaccgcggc cgcgtgatcg aatatgtgcg cgataaatac
    16021 ggcgctcagg cggtcagcca gattgttacc ttcggcacga tgtcgtccaa agcggtcatc
    16081 cgcgacgtgg ggcgcgtgtt ggagctgccg tttatgctgt gcgacaaact gtccaagctg
    16141 attccgttgg aagccaacaa acctctgagt ttggaaaaag ccatggaggc cgagccgcag
    16201 attcaggaat tgatcgaggt ggaagaagcg gacgaactga ttacactggc gaaaaagctg
    16261 gaagatttga cgcgcggtct gggtatgcac gcgggcggcg tgttgattgc gccgggcaag
    16321 atttccgatt acagccccgt gtatcaggcg gatgaatccg cctcgcccgt atccatgtac
    16381 gacaagggtg acgtggaaga cgtgggcttg gtgaaattcg actttttggg tctgcgcaac
    16441 ctgaccatta tcgaaatggc gcagaacaac atcaaaaaca ccaccggcga catcatcgat
    16501 gtcggcaaaa ttcctctcga cgaccaagcc gcctaccaaa tcttccgaga tgcgaacacc
    16561 accgccgtgt tccagttcga gtcgaccggc atgaaaaaaa tgctgaaaac ggcgcacacg
    16621 accaagtttg aagaactgat tgccttcgta tcgctctacc gccccggccc gatggacaac
    16681 atccccgact tcgtcgcgcg tatgaagggg caagaattcc aatatatcca tccgctgctc
    16741 gagggcatcc tcgcgccgac ctacggcatt atggtgtatc aggaacaagt gatgcaggcg
    16801 gcacagatta tcggcggcta ctcgctcggc ggcgcggact tgctgcgccg cgcgatgggt
    16861 aagaaaaagc ccgaagaaat ggtgaaacac cgcgaaatct tcgccgaagg cgcggcaaaa
    16921 caaggcattt cgcgcgaaaa atccgacgaa atcttcaact atatggaaaa attcgccggc
    16981 tacggtttca acaaatccca cgccgccgcc tacgccctga tttcctacca gaccgcatgg
    17041 ctcaaggcgc actaccccgc cgaatttatg gcggcgacca tgtcgtccga attggacaac
    17101 accgaccagc tcaagcattt ctacgacgac tgccgcgcca acggcatcga gttcctgccg
    17161 cccgacatca acgaatccga ctaccgcttt acgccgtatc cggacatgaa aatccgctac
    17221 gcgctcggcg cgattaaagg cacgggcgaa gccgccgtcg aatccatcac cgccgcgcgg
    17281 caaagcggcg gcaagtttac cggcctgttg gacttctgcg agcgcgtcgg caaagaacac
    17341 atgaaccgcc gcaccctcga agccctgata cgcggcggcg cgttcgacag tatcgaaccc
    17401 aaccgcgcca tgctcttggc gaacatcgac ctcgctatgg acaacgccga ccaaaaagcc
    17461 gccaacgcca atcagggcgg gctgttcgac atgatggaag acgccatcga accggtgcgg
    17521 ctcatcgacc tgccgaggtg gagcgaatcg gaaaaactcg ccgaagaaaa aaccgtcatc
    17581 ggcttttacc tgtccggcca cccgttcggc ccgtatgccc aagaagtccg ccaaatcgca
    17641 ccgaccaaat tagaccgtct gaagccgcaa gacagcgtgc gcctcgccgg attcgttacc
    17701 gccgtacgca ccatgatggg caagcgcggc aaaatcgcct tcgtcagcct tgaagatttg
    17761 agcggacagg ttgaaatcat ggtcggcggt cagacgttgg aaaactgcgc cgactgcctc
    17821 aaagccgacc aagtgctgat tatcgaatcc aaagtcagcc gcgacgacta cggcggcggc
    17881 gacgggctgc gtattctggc aaaccaagtc atgaccctgc aaacggcacg cgaacgctac
    17941 gcccgcagcc tcagcctcgc cctcgccccg catcacgaca tcggcggact ggtacggctg
    18001 ctcgccgccc accaactgcc cgacacgccg cgcatcccgc tgcaactgtc gtatgccaac
    18061 gaaaaagcgt cgggcaggct tcaagtgccg ccgaaatgga cggttacacc gagctccgca
    18121 ttgttcggcg aactggaaac attgctcggc agccggtcgg tgcgcgtcaa ctggtaaccc
    18181 aaaatataaa tgccgtctga agcccaaaaa ccggtttcat tcgtacttta ttcgaatgat
    18241 tgaataaaag taactgccaa gaaaaacgta ttttttggtt atttcgccag tctaaataga
    18301 gcaaccggga cgattgatat ccgtgtgcat gacacagaca gcaccaaagg gaaaaacggc
    18361 attttccaaa gtatcggtat caaaaccgcc ctttcactcc aaaaatacca aatcgacaaa
    18421 ccgggcaaag aaatcagacc gtgccgcctg aaaaaacgcc cacccgtccg ttaaattaac
    18481 ctatccctgt ttcagacggc ctgaagcagg gatttttata tcaaaataaa atgagaaagg
    18541 gagcaataac ccttaggtag ctcttgttat tttccgatgc aaaacaaagc agtcatatat
    18601 ttaattcccc ctacctctgc caagccttcc tcaaatattc gacgcaatcg gtcagcgagt
    18661 agaacgagac attgccgtgg tcggcattcg gatattcccg gaaaaagacg gcggccccgt
    18721 gcctgtccaa ctctgccgcc atgcgttcgg acagtcctgc catatcgcgt tcttccctgc
    18781 gtttgcaacc gctgccccgt tctagcgcgc cgatgttgag gcagacatcg atgccgtcca
    18841 gccggttttc agacggcata aaatcgagta tccgcctgtt gtgccaccaa atcgaggggg
    18901 atacgagcca atgccgtctg aaacggcggt gggaaagcag ggaatacagt ccgaacagtg
    18961 cgccgaacga gtgtccgaat acggcggttt cattgcggtt gagggtgtag cggctttcta
    19021 aaaaggcggt cagctcgctg tcgataaagg cggcgaagcg gtctgcctgt ccgaactgct
    19081 gccgttcgtc tgctgtggcg ttgtctccaa gcggcggcgt gtagtcggcg gcacgttgtg
    19141 ccaaatcgcg cacactgcct gtcgtgtagc cgataccgac aatcaggcag ggggcgttgc
    19201 ttcgggtaac ggggttgttc atcagcgact gcatgatgtt gaaaagtgcg gggaaaaagg
    19261 cttcgccgtc gaggacaaag aggacgggat agccttcaga cggtatttcg ccgagtgttg
    19321 ccgtctgaat ccgatagatt cgccccgtgc aggtggattt gatttcggtt tcaaaggctt
    19381 ggggcagtat ggcaggttgg aatgtgtcgg tcggtatggg tttcatgatg ttcggcttgt
    19441 gggtcagact ggcggcaagc ggtaaaaccg aaccggcaaa gcggtctgcc tgccggttcg
    19501 gtctattttc cttcgcaatg ccatactcca gttgtgagag catagggtat gccgcgcagc
    19561 ttgttgtagt ttgatgatgc tgcggctgcc gtttcagacg gcatattggt ctttaaaaac
    19621 tgtaacgcag gtttgccgtc aggctgcgct ccgaaccggg aatgttaaag gtgctctcgc
    19681 tgccgacgcg ggcgtagtaa tggcggttga agatgttgtc ggcgttgatt tgcagcttca
    19741 gtttgggcgt gaagcggtat gccgccatcg catcgaacgt ggcataaccg cctgcatgta
    19801 tccctgcaga tgaagtaatg ccgctcatcg cgttcacgcc gccgccgatg gtcagcccgg
    19861 acgtaacttg gtaagtcgtc cacaggtttg cgctgtgttt gggcatcagc aggaagatgc
    19921 cttcgtcgcg cgaattggag gcggttttga tttggctgtg caggtagctg taacctgcat
    19981 ggatttgcca tttcggtgtc atcgcgccgc tgatttcggt ctcaacacct tccatcacgc
    20041 gtttgcccaa tgcggcgtaa cgggtttttt tgttgtttga gtccagcggt gcggcggcgt
    20101 ttttatcctt catgcggtag aacgaaaccc gggtattgag gcggtcgtcc atgtagctgc
    20161 ctttgtagcc gatttcaaac tggttgcctt cgcgcggttt gagcagcttg ccgtcggtgc
    20221 cgatgctggt ttgcggtgtg tagagttggg aggcggaagc gtacaggctg ttgctgccgt
    20281 ctatatcgta aaccgcgccg gcgtagcttg taaatttggt tttcgaagct ttatgcaggg
    20341 ttttgccgtc gcccgactcg attttgtgat gtcctacacg tccgcctgca atcaacgaca
    20401 aaccttccag aggacggaac accgtcttgg catacaaacc ggtttcgtcg aggttttctt
    20461 cggtaacgga gtgattgaaa cctttgtttc cggcgcgggc gttctgaagt atgccgttat
    20521 aaggcaaagc gcggaaacca tctaaagcga cgctttttga caaagtcgaa cgcccttgtt
    20581 cattagtact gcgcaagcgg ttgtagtctg caccaatcac aaattcgttg gcggtgttgc
    20641 ccaaggcaaa cggacggctg taacttgcgt caaccgcaaa ggctttttgt ttaatgtccg
    20701 tacccaaacc cgctacgtcg gcttgtccgg tattgttgag tttgctgccc gcaaacgtat
    20761 aattggaatc ggctttccga tcggaatagc gcataccgac tttgccgtag ccgccgttgc
    20821 cgaagtaatg tttcaaatcg gcgaacacgt cgtggctgtg cattttaaat ttgttccaat
    20881 ccgcgccgac aaatacgtgt tgcggcaggg acggtaattt gttattggca tcggcaggca
    20941 ggccgttgta cggcgcgagg cggcgttgct ggtaaagata gcccgcgccc aaaaccgtat
    21001 cggggttgat gtcccaatcc gccgccgcgt agaaggtttc gcaccggttg tttttctcgg
    21061 cgggacgcgg agacgcgccg acggtctgcg ccatcacgcg gccgcgcacg ctgccgtctg
    21121 aattgaggct gcccgatacg tccgcctcgg ctttatattg tttgtgcgta ccgaaccctg
    21181 ccgccgcatg accttggaac gctttggtcg ggcgtttgcg caccagattc acgatgccgc
    21241 ccatctcgcc gctgctgtcg aacagtccgc tcggcccgcg catcacttcc acgcggtcga
    21301 aggcgaacag gttgggcagc gtgccgttga tactctgcat ctgcgcgggc aggccgtcga
    21361 tgttgtattc gctgtattcg taaccgcgcg cgtaaaccga agagcgtccg tcgtcgttgc
    21421 tcaacacgcg caggccgggc gttttgcgtg ccaactggtc aaacgtatca acattgcggt
    21481 ctttgacctg ctggttggta atgatgctga cggattgcgg aatttcgcgc aaagaagcgg
    21541 ggatttttgt accgacggtg gcggcaaacg agctgtaatc gccgtttttc tcggtggcaa
    21601 tcgcgttgta agaacgctga cccttaatat ggacggtttc caaaccttcc gtttgtgcgg
    21661 caaaaaccga agacgagagt gctgccaaaa ccgtggcggc ggtcatattg atgcggaaaa
    21721 ctgacataaa ctgtcccatt cacataaatg ataatggttc tattttaata aagcgcaacg
    21781 cggcttgttc ggaaaaacat atcgcgcagc cgacaaattt tgtcgaaaat gcgacacgtc
    21841 tgcgttttcc gcataaaatt tgctttttta ctgcaaccaa cctgctatga ccacgcccaa
    21901 actcatcatc ttcgactggg acggcacgct tgccgatacg acccaaccca tcatcgacac
    21961 catgcgccgc agcttcgccg aatgcggttt tccgccgccc gaagcggaac gcgtccgcag
    22021 cctgattggc tacagcctgc ccgaaatcat ccgcaccctg ctcgaaatgc cgtctgaaac
    22081 cgccgttgcc gacatcacac gcacttattc cgcacattac ctcaatccca acaaccgcaa
    22141 tatgtcctta tttcccgatg ccctgccctg tctggacaag ctcaaagcac aaggatactg
    22201 gcttgccgtc gccacgggca aagggcgggc gggtttggac aacgccatca gtcaaaccgc
    22261 caccggcggc tattggctcg ccaccgcctg cgcgggggaa tatccctcca aaccctcgcc
    22321 cgaaatggta ttcggaatct gcggcgaact gggactcgac ccgaaagagg cattggtcgt
    22381 cggcgatacg gcgcacgacc tgcatatggc ggcaaacgca ggcgcggcgg cagtcggcgt
    22441 ggccaccggc gcacattcgc gcgaacagct ccttagcgca ccgcatctcg ccgtattgga
    22501 cggtttgtcc gaactgcccg gttttcttgc acaacattac gcctgattgg tttccgcatc
    22561 cggcacacgg caaaaatgcc gtctgaagcc tgttcagacg gcatttgtgt tgcccaaaca
    22621 ttcaacgcct gcgtcaacgt ttgcacaaat cgggtttggt ttcgccctcg cggcgcaact
    22681 ctttgggcag gacgaacaca atgctttctt ccgcaccctc gccttcgcgt acggtttcgt
    22741 gcccccatcc gcgtatggtt gccagtactt cccgcaccaa cacttcgggc gcggacgcgc
    22801 ctgccgttac gccgactttg tttttgccct caaaccatgc gcgttgcagg tagcctgcat
    22861 tatccaccat atacgcatcg attccgcgcg atgccgccac ttcgcgcaag cggttgctgt
    22921 tggacgaatt gggcgaaccg accacaatca cgatgtcgca ctgttctgcc aactctttga
    22981 cggcggtttg ccggttggtc gtcgcatagc agatatcttc cttgtgcgga ttgcggatat
    23041 tggggaaacg cgcgttcagc gcggcgatga tgtctttggt ttcatcgacc gagagcgtgg
    23101 tttggctgac ataggcgagt ttgtcggggt ttctgacttc gagttttgcc acatctccga
    23161 ccgtttcgac caaaagcatt ttgcccggcg caagctgccc catcgttcct tcgacctcga
    23221 cgtgcccctt atgcccgatc atgatgattt cacagtcttg ggcatccagt cgggcgactt
    23281 ccttatgcac tttcgtcacc agcgggcaag tcgcatcaaa cacgcggaaa ccgcgctccg
    23341 ccgcttcttg ccgcaccgcc ttcgatacgc cgtgtgccga ataaaccagt gtcgcgcccg
    23401 gcggcacttc cgccaagtct tcaataaaca ccgcaccttt ttcacgcagg ttgtccacga
    23461 cgaatttgtt gtgaacgact tcgtggcgca cataaatcgg cgcgccgaac tcttccaaag
    23521 cacgttcgac aatactgatt gcccgatcca caccagcgca gaagccgcgc ggattggcaa
    23581 ggatgatggt tttctcgttc ataagcccgg tatttcgttt tcagacggca tcaatatttt
    23641 tcttcttggg ttttacggtg gacgatgttg tccaacactg ccaacaccgc gccgacgcag
    23701 ataaagctgt cggcaatatt aaaggcggga taaaaccaat tttgccaata aaacaataag
    23761 aaatcgacga catgaccgtg tatcaggcgg tcgatgacat tgcctaacgc accgccgata
    23821 atcatcgccg cacccgtttt gccgagggtt gcaaactcat cgcgcaagat ggcgcgtacc
    23881 aaatacgcgc tcaccgccac cgccagcacc aaaaaaaagt atttttgcca gccgccctga
    23941 tcggcaagga agctgaacgc cgcacccggg ttgtacacca aggtcaaatc gaaaaaggaa
    24001 ggaatgacat tgacgcgttc ccgatactga aacgacgaca gcaccgccca cttcgaccac
    24061 tggtccagca cgatggcggc aagtgccaat acccaatagc gcgttttact tgaaacagat
    24121 gaagacatat ttttcaacag ccggtaaaag agtaccattt tacccgaaaa ccccctttcc
    24181 tgtacccgaa acggcaaatg ccgtaatctt aaaacccgtc attcccgaca acaccgtaat
    24241 ctcgaaaccc gtcattcccg cgtaggcggg aatccagacc tgtccgcaca gaaacttatc
    24301 ggataaaaac agttgcccaa accccgcgtt ctatagtgga ttaaattcaa accagtacgg
    24361 cattgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgttgcct tgtcctgatt
    24421 taaatttaat ccactataga ttcccacttc cgtgggaatg acggttcagt tgcattccga
    24481 caacaccgta atcttgaaat ccgtcattcc cgcgc
//
LOCUS       DAWHRS010000010        23893 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWHRS010000010 DAWHRS010000000
VERSION     DAWHRS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.010"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WIB82_000292"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WIB82_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ3841656.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(2325..2415)
                     /locus_tag="WIB82_000293"
     tRNA            complement(2325..2415)
                     /locus_tag="WIB82_000293"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ser,seq:gga)
     gene            complement(2454..2540)
                     /locus_tag="WIB82_000294"
     tRNA            complement(2454..2540)
                     /locus_tag="WIB82_000294"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2504..2506),aa:Leu,seq:cag)
     gene            complement(2576..2662)
                     /locus_tag="WIB82_000295"
     tRNA            complement(2576..2662)
                     /locus_tag="WIB82_000295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2626..2628),aa:Leu,seq:cag)
     gene            2751..5126
                     /gene="rnr"
                     /locus_tag="WIB82_000296"
     CDS             2751..5126
                     /gene="rnr"
                     /locus_tag="WIB82_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ3841657.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPVHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDVIDADAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWISDGIDHPYKAQIDTLYKLFKILQKKRFERGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKPVPIKVKKRKGKS"
     gene            complement(5465..6928)
                     /gene="guaB"
                     /locus_tag="WIB82_000297"
     CDS             complement(5465..6928)
                     /gene="guaB"
                     /locus_tag="WIB82_000297"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ3841658.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(7075..9633)
                     /gene="glnD"
                     /locus_tag="WIB82_000298"
     CDS             complement(7075..9633)
                     /gene="glnD"
                     /locus_tag="WIB82_000298"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ3841659.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAALKTLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLIPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATNLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFNLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAVKTVKQLSGILTPMLRSRVSSAPMRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQCNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTKEESDLLAWLVENHLLMSTVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILFKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            complement(9682..10002)
                     /locus_tag="WIB82_000299"
     CDS             complement(9682..10002)
                     /locus_tag="WIB82_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3841660.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            10018..10287
                     /locus_tag="WIB82_000300"
     CDS             10018..10287
                     /locus_tag="WIB82_000300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841661.1"
                     /translation="MVKVKHLLLRILLHIKAKKRGGGSHTQTHTHQEERGKIKTDWET
                     ANKQSRFHIGIIKQKPLSGIDSNQFRFYSQTEKEKRHIRLYGYAV"
     gene            complement(10327..10800)
                     /gene="bfr"
                     /locus_tag="WIB82_000301"
     CDS             complement(10327..10800)
                     /gene="bfr"
                     /locus_tag="WIB82_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ3841662.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(10828..11292)
                     /gene="bfr"
                     /locus_tag="WIB82_000302"
     CDS             complement(10828..11292)
                     /gene="bfr"
                     /locus_tag="WIB82_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ3841663.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVSRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            11647..11850
                     /locus_tag="WIB82_000303"
     CDS             11647..11850
                     /locus_tag="WIB82_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841664.1"
                     /translation="MPSEGLSDGMPARTDAAWSGCRFEGVFNAKGNLSYDVFAAGALK
                     KPGILGRSARLPGSISAIYSKPY"
     gene            11868..12335
                     /locus_tag="WIB82_000304"
     CDS             11868..12335
                     /locus_tag="WIB82_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ3841665.1"
                     /translation="MKIENIDIISPELFPQETFNETEAFGALVWLWAVSPIYQHAGVQ
                     EAAVNILPVLKNGQFALFSSNGHPVAYCTWAYFDEETEWQYLQSNDVLRHSENWCSGN
                     RMWLINWFAPFGDSRMMKRILVHLFPKREIRWLYHRGSEKGKRIMRFPALSKQ"
     gene            complement(12376..12732)
                     /locus_tag="WIB82_000305"
     CDS             complement(12376..12732)
                     /locus_tag="WIB82_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841666.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPPPDDSVYWKYSRIEQEYPAMM
                     SKNINLRIYSYEEYSKQIDDLFAKEKKDWKECGYDPIGGGGGSKADACMRKRGWYRVG
                     DDIYPENKKYEWPREN"
     gene            complement(12729..13067)
                     /locus_tag="WIB82_000306"
     CDS             complement(12729..13067)
                     /locus_tag="WIB82_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841667.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPNPWDAASFWELKNYANPYPGS
                     ASAALDQYPSKARRRQLKDMQECGYDPIDGGKSEADACLRKKGWCRKGFDPYPENKKY
                     EWPREEGKTK"
     gene            complement(13064..13426)
                     /locus_tag="WIB82_000307"
     CDS             complement(13064..13426)
                     /locus_tag="WIB82_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ3841668.1"
                     /translation="MKYIVSISLAMGLAACSFGGFKPPPDDSAFWRLTNYAKLYPGLT
                     SATLDQYPPEERRRQLHDSFAREKKDWKECGYDPIGGGGGSEADACMRKRGWYRVGND
                     IYPENKKYEWPREEGKTK"
     gene            complement(13423..20244)
                     /locus_tag="WIB82_000308"
     CDS             complement(13423..20244)
                     /locus_tag="WIB82_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ3841669.1"
                     /translation="MNKRCYKVIFNKKRSCMMAVAENVHRDGKSMQDSEAASVRVTGA
                     ASVSSARAAFGFRMAAFSVMLALGVAAFSPAPASGIIADKSAPKNQQAVILQTANGLP
                     QVNIQTPSSQGVSVNRFKQFDVDEKGVILNNSRSNTQTQLGGWIQGNPHLARGEARVI
                     VNQIDSSNPSLLNGYIEVGGKRAEVVVANPSGIRVNGGGLINAASVTLTSGVPVLNNG
                     NLTGFDVSSGKVVIGGKGLDTSDADYTRILSRAAEINAGVWGKDVKVVSGKNKLDFDG
                     SLAKTASAPSSSDSVTPTVAIDTATLGGMYADKITLISTDNGAVIRNKGRIFAATGGV
                     TLSADGKLSNSGSIDAAEITISAQTVDNRQGFIRSGKGSVLKVSDGINNQAGLIGSAG
                     LLDIRDTGKSSLHINNTDGTIIAGKDVSLQAKSLDNDGILTAARDVSVSLHDDFAGKR
                     DIEAGRTLTFSTQGRLKNTRIIQAGDTVSLTAAQIDNTVSGKIQSGNRTGLNGKNGIT
                     NRGLINSNGITLLQTEAKSDNAGTGRIYGSRVAVEADTLLNREETVNGETKAAVIAAR
                     ERLDIGAREIENREAALLSSSGDLHIGSALNGSRQVQGANTSLHNRSAAIESSGNIRI
                     ATKDLQNTNEHLRFHTEETHREHRIEYQAEGRTERYPEGSQKELGWEIFEDESLHMRT
                     PDGSPHSVWYKYDYERITAESKITESKPGQIISGGNLVLDAAKLKNHNSRIIAGGRLI
                     VGTPESALDNDETLGTKTITDKGDLHRYHRHHKKGRDSTGYSRSPYEPAPEVSSIRMG
                     ISAYKGYAPQQASDIPGTVVPVVAENGIHPTFTLPNSSLFAIAPNNKGYLIETDPAFT
                     DYRKWLGSGYMLAALQQDPNHIHKRLGDGYYEQKLVNEQIAKLTGYRRLDGYTNDEEQ
                     FKALMDNGITIAKELQLTPGIALSAEQVARLTSDIVWLENETVTLPDGTTQTVLKPKV
                     YVRARPKDMNGQGALLSGSVVDIGSGAIENRGGLIAGREALILNAQNIKNLQGDLQGK
                     NIFAAAGSDITNTGSIGAENALLLKASNNIESRSETRSNQNEQGSVRNIGRVAGIYLT
                     GRQNGSVLLDAGNNIVLTASELTNQSEDGQTVLNAGGDIRSDTTGISRNQNTIFDSDN
                     YVIRKEQNEVGSTIRTRGNLSLNAKGDIRIRAAEVGSEQGRLKLAAGRDIKVEAGKAH
                     TETEDALKYTGRSGGGIKQKMTRHLKNQNGQAVSGTLDGKEIILVSGRDITVTGSNII
                     ADNHTILSAKNNIVLKAAETRSRSAEMNKKEKSGLMGSGGIGFTAGSKKDTQTNRSET
                     VSHTESVVGSLNGNTLISAGKHYTQTGSTISSPQGDVGISSGKISIDAAQNRYSQESK
                     QVYEQKGVTVAISVPVVNTVMGAVDAVKAVQTVGKSKNSRVNAMAAANALNKGVDSGV
                     ALYNAARNPKKAAGQGISVSVTYGEQKNTSESRIKGTQVQEGKITGGGKVSLTASGAG
                     KDSRITITGSDVYGGKGTRLKAENAVQIEAARQTHQERSENKSAGFNAGVAIAINKGI
                     SFGFTAGANYGKGYGNGDETAYRNSHIGSKDSQTAIESGGDTVIKGGQLKGKGVGVTA
                     ESLHIESLQDTAVFKGKQENVSAQVTVGYGFSVGGSYNRSKSSSDYASVNEQSGIFAG
                     GDGYRIRVNGKTGLVGAAVVSDADKSKNLLKTSEIWHKDIQNHASAAASALGLSGGFS
                     YSPKPTSGQYSTKKEAEIGKIGGKPVSLMRFDQVSAKDDELNEKYRSERIEKGETFKE
                     ANLNQNNAGGLKFGLKQNDIHSNDKYALAKMGLGNLLGNAKESESRQSITRSVISEGD
                     WQIASAQGRKNIAGIEKGTSSAHKALAKADREGLLKEVELNRDVAKEFINETLIGGIA
                     DEAYRSQFIAEHRLMTFKMDENGEPIEDKQLEEDINKQFDNSVKLKKEFASFKDYWEA
                     YKAIGGNIYELREVSDQERKNLKTARYTDPETGKTVEKIVVGVNGIFNNIQAAAKFAA
                     QQYVGRFNPEKNRYERTYENVYFLHNPETNGRGFSKLPEIAVAAFHKMLEGAKIGNKT
                     VIGLSNSGLALGNIMEDYGKDKNGLFVGSHSRGTLVVDNVLNTLNTQANRDKKILSNT
                     ELKMVGPAANVVRADKRLFQLQQGVTTPRTADFARQSIQIENHELDLIGMLIGRNPAT
                     VGTNTRQKSQWQAIRDIIGDYTSPHNCYGMANKQCVTDGYRDPENKQTQSPTGVFERG
                     VSNEIEIMYRPVRIYDLQHPKGKTK"
     gene            complement(20754..21737)
                     /gene="lipA"
                     /locus_tag="WIB82_000309"
     CDS             complement(20754..21737)
                     /gene="lipA"
                     /locus_tag="WIB82_000309"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ3841670.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(21730..22353)
                     /gene="lipB"
                     /locus_tag="WIB82_000310"
     CDS             complement(21730..22353)
                     /gene="lipB"
                     /locus_tag="WIB82_000310"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ3841671.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(22355..22630)
                     /locus_tag="WIB82_000311"
     CDS             complement(22355..22630)
                     /locus_tag="WIB82_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ3841672.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            22815..23885
                     /locus_tag="WIB82_000312"
     CDS             22815..23885
                     /locus_tag="WIB82_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ3841673.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
BASE COUNT         5134 a         5872 c         6507 g         6380 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgta gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 ccttgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg acacgccggg acacgccgac ttcggcggcg aagtagagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caacaaaatc gacaagccgt ccgctcgtcc gagctgggtt atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt acgcttcagg
      601 gttgagcggt ttcgccaaat tggaagaaac cgacgagagc aacgacatgc gtccgctgtt
      661 cgatactatc ttaaaatata cgcctgcacc gagcggcagc gcggacgaaa cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggtatcg gtcgtatctt
      781 gaacggacgc atcaaacccg gccaaaccgt tgccgtcatg aaccacgatc agcaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggtttg gaacgcgtgc cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtatcgaa gacatcggta tcggcgtaac
      961 catcaccgac aaagacaatc ccaaaggcct accgatgttg agcgtggacg aaccgacgct
     1021 gacgatggac tttatggtca acaccagccc gctggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagatacc gccgatgccg acgtgttccg cgtatccggg cgcggcgagc tgcacctgac
     1201 cattttgctg gaaaacatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccgtat gaaaacctga ccgtggatgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgacggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aatttatgac cctgacgcgc ggggtcgggc tgatgagcca
     1501 cgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg
     1621 ccgtatgttc gtatcgccca acgacaaaat ctacgaaggc atgattatcg gcatccacag
     1681 tcgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgtgc
     1741 cagcggtacc gacgaagccg ttcgcctgac cacgccaatc aagctgacgc tggaaggtgc
     1801 ggttgagttt atcgacgatg acgaactcgt tgaaatcacg ccgcaatcca tccgtctgcg
     1861 caagcgttac ttgagcgaat tggaacgccg ccgccacttt aaaaagctgg attgatgttt
     1921 actggattaa tgtttaaatg atacgcgatg ccgtctgaaa aatttcagac ggcatttttt
     1981 attcggacgg gctttgcggc ttcttgaagc tgtttcagac ggcgtttttc ctacccaatc
     2041 aagaaactgc cgccattttt ccagcggtat atcgccccgt cgcgtttcgg tatcgggttc
     2101 ggcttcccgg cagcatgtcg aacaatgccg tttgaaggaa tacgcctttt gagtccttac
     2161 aggctgagga agagggtaaa acataccgca aaacatacca caaaaaacgg taacggatag
     2221 ttgtaagcgg ttgatgacga ttatagacaa taacgggttt tcccaatgaa attattgttt
     2281 gaataataaa aaatcccaaa ccgtaaaagt ttgggatttg tatttggcag agaggaaggg
     2341 attcgaaccc tcgatacgct attcacgtat acacgctttc caggcgtgcg acttaaacca
     2401 ctcatccacc tctctaatgg cggaaattat cccaatcggg ataatttatt atttggtgcc
     2461 cgggagaaga ctcgaacttc cacacccatg aggataccag cacctgaagc tggcgcgtct
     2521 accaattccg ccacccgggc aatctaaata ttgaaataaa gcaaagcatt tgatttggtg
     2581 cccgggagaa gactcgaact tccacacccg tgaggatact agcacctgaa gctagcgcgt
     2641 ctaccaattc cgccacccgg gctttgcttt atttgctgtt ttgcggtaca gtgtcctgcc
     2701 gcaaagaagt cgctattata tagttcataa agagaatgtc aacagtccaa atgaataaaa
     2761 atattaaatc tttaaattta cgggaaaaag acccgttttt aagtcgtgaa aaacagcgtt
     2821 atgaacatcc tttgcccagt cgggaatgga taatcgaatt gttggagcgc aaaggtgtgc
     2881 cttcaaaaat cgaatcgctt gcgcgcgagc tgtcgattac ggaagacgag tatgtctttt
     2941 ttgaacgccg tctgaaggcg atggcgcggg acggtcaggt tttaatcaac cgtcggggcg
     3001 cggtttgcgc ggcggacaaa ttggatttgg tcaaatgccg cgtcgaggcg cataaggacg
     3061 gtttcggttt tgccgtgccg ctcacgcccg ccaaagacgg tgattttgtt ttgtatgaac
     3121 gccagatgcg cggcattatg cacggcgata ttgtcactgt tcgtcctgcc ggcatggacc
     3181 gtaggggccg ccgcgaaggg acagttctgg atattgtcga acgcgcgcaa agcaaagtgg
     3241 tcggccgttt ctatatggat aggggcgtgg cgattttgga gccggaagac aagcgtctga
     3301 accaaagcat cgtattggaa ccggacggcg tggcgcgttt caaacctgaa tccggtcagg
     3361 tcatcgtcgg cgaaattgag gtttatcctg agcaaaaccg gccggcagtg gcaaaaatca
     3421 tcgaagtttt gggcgattat gccgacagcg gcatggagat tgaaattgcc gtgcgcaagc
     3481 atcatttgcc gcaccaattc agtgaagcgt gtgccaaagc tgcgaaaaaa attcccgtcc
     3541 atgtacgcaa aagcgatttg aaaggccgcg tcgatttgcg cgacctgcct ttggtaacga
     3601 tagacggcga aacggcgcgc gatttcgacg acgcggtgtt tgccgaaaaa gtcggacgca
     3661 attaccgtct ggtcgtggcg attgcggatg tcagccatta tgtccgccct gacgatgtga
     3721 ttgatgcaga tgctcaagaa cgcagtacca gcgtatattt cccgcgccgt gtgattccga
     3781 tgctgccgga aaacctgtct aacggcattt gctcgctcaa tcccgatgtc gagcgtttgt
     3841 gtatggtgtg cgatatggtc gttacctatg cgggcaatat caaagaatac cgcttctacc
     3901 ccgccgtaat gcgctctcat gcccgcctga cctacaacca agtttggaaa tggatttcag
     3961 acggcatcga ccatccgtac aaagcccaaa tcgacaccct ttacaaactc ttcaaaatcc
     4021 ttcagaaaaa gcgtttcgaa cgcggcgcgg tggagtttga aagcgtcgaa acccagatga
     4081 ttttcgatga caacggcaaa atcgaaaaaa tcgtccccgt tgtccgcaac gatgcccaca
     4141 agctgattga agaatgtatg ctggcggcga atgtttgcgc agcggatttc ctgttgaaaa
     4201 acaagcatac ggctttgttc cgcaaccatt tgggccccac gcccgaaaaa ctcgccaccc
     4261 tgcgcgagca gctcggtctg ttggggcttc aacttggcgg cggcgacaac ccgtcgccga
     4321 aagactatgc cgcgcttgtc gaacaattca aaggcagacc tgatgccgaa ttgctgcaag
     4381 tcatgatgtt gcgctccatg cagcaggcgg tttacgaacc gcattgcgac ggacactttg
     4441 gtcttgccta cgaagcatac gcccacttca cctcgcccat ccgccgctat cccgacctga
     4501 ccgtacaccg cgccatcaaa gccgtgttga atcagcaaac ctacacgcca aaaaaaagct
     4561 ggcaggcttt gggcgtgcat acctcgttct gtgagcgccg tgccgacgac gccagccgcg
     4621 acgtggaaaa ctggctgaaa acctattata tgcgcgataa ggtcggcgaa gtattcgaag
     4681 gtaaaatctc cggcatgacc agttttggta tctttgtaac actggacggc atccacattg
     4741 acggcttggt gcatatcagc gatttgggcg aagactattt caacttccgc cccgaaatca
     4801 tggcaatcga aggcgaacgc agcggcatcc gtttcaacat gggggacagg gttgccgtcc
     4861 gggtcgcccg tgccgatttg gatgacggaa aaatcgattt tgtcctgatt gccgggggga
     4921 gcggcagggg gcggaaagtt aaatcatccg cgtctgccaa accggcaggg acggcgggga
     4981 aagggaagcc gaaaaccgcc gccgagaaaa aaacagcccg aggcggcaaa gtaaggggaa
     5041 ggggcgcgtc tgccgccgca gaatcgagga aaaaggcaaa gaaaccggtt ccgattaagg
     5101 taaaaaaacg gaaaggcaaa tcataatgct gacggggcgg cttggggagg gcggggcata
     5161 ctttcccgaa gccgccgccc gattttttga tatggcggcc ggcgcatcat ttcaggccaa
     5221 tcgaaacgcc cgtattgaaa gattgcgttt atttccaccg ccgttttaaa ggccggcggt
     5281 attcggcaga cggggcgcaa acggcgttca gacggcattt tcattctttc ggcgtgtccg
     5341 tccgaatggc tttgcccgtc cgcgcaatca gcccgtgcgg cttgccttga acggacaaaa
     5401 aatgccgtct gaaacccgaa aatcaggttt cagacggcat tttgccttga aaaggctgtt
     5461 caaatcagcg gtggtagttc ggtgcttctt tggtaatttg aacgtcgtga acgtgcgatt
     5521 cgctcatacc tgcggaagtg atttccacaa attctgcttt ttcgtgcatt tcggcaatat
     5581 tggcgcaacc caaatacccc atgctggagc gcagtccgcc ggtcagttgg tggatgatgt
     5641 tcacaatcgg gcctttgtag ggaacgcggc cttcgatgcc ttcggggacg tatttgtcgg
     5701 tgctgtcggt tttgtcttgg aagtagcggt cggcagaacc ttggctcatc gcgcccaatg
     5761 aacccatacc gcgataggat ttgtacgagc ggccttggta gagttcgatt tcgcccggcg
     5821 cttcttccgt gcctgcaaac ataccgccga gcatgacgct gtacgcgcct gcggcgaggg
     5881 ctttggcgat gtcgccggag aagcggatgc cgccgtcggc aatcagcgga acgcccgtgc
     5941 ctttgagggc ttcggcaacg ttgtgaatgg cggtcagttg cggcacgccg acacctgcca
     6001 cgatacgggt ggtgcaaatc gatcccggac cgataccgac tttgacggca tccgcgccgg
     6061 cggcgaccaa atccaatgcg gctttggcag tggcgatgtt gccgccgatg acttggatgt
     6121 gcggataggt ttctttcacc catttcacgc ggtcgatcac gccttggctg tgcccgtggg
     6181 cggtatcgac gacgatgaca tcgactcctg cttctaccaa tgcttttacg cgctcttcgg
     6241 tgtcgccgcc ggtgccgact gccgcaccga cgcgcagacg gccctcggag tctttgttgg
     6301 cattgggaaa ttcggtggtt tttaaaatat ctttgacggt aatcagacct ttgagttcgt
     6361 ctttttcgtt cagaaccaaa acgcgctcga ctttgtgcgt gtgcatcagt tcgcgcgctt
     6421 cgtctatgct tgtgccttcg gggacggtaa ccagacgttc gcgcggggtc ataatggcgg
     6481 aaacgggcaa atcgacgcgg ttttcaaaac gcaggtcgcg gttggttacg atgccgacga
     6541 ctttgccgtt ttcaacgacg ggcaggccgg acattttgcg tttgcgctgc gcgcgcattt
     6601 ccaagacttc gcggatgagc gtcgtcggcg caacggttac ggggtctttg accacgccgc
     6661 tttcgtggcg tttcactttg gaaacggcgc gcgcctgcat ctccggcggc atatttttat
     6721 ggatgatgcc gatgccgcct tcctgtgcca tcgaaatggc gaggcgcgcc tcggtaacag
     6781 tatccatcgc ggcggaaagc agggggaggt tgagcgtgat ttcgcgggtg agcttggttt
     6841 gaagtttaac gtctcgcggc agcacggtcg aatgtgcggg aaccaacaaa acatcgtcga
     6901 aagtataggc tttttctacg atacgcataa tgctcggtct ttcagtttgt gcaagatgca
     6961 cggcattata gcacgttacc ggcggcttga cagtttatat tgtttgcaat atcccccgcc
     7021 ttcagacagc gtacggcgcg gcggcgggat accgtctgaa aaccggcagg gtgcttatgt
     7081 caactgttcc agcaaagcct gcttcaatga ggactggatt tttgggtttt tcaggtcggg
     7141 gctaaaaacg gtaaaactgt cttcggcgcg ttcatccagt gtggagattt tggcatagcg
     7201 caggctgacg ttgtgggcga aaaagacttc cgccatatcg gcgagcagga aggggcggtt
     7261 gacggcggta atttcgacgg agtaccagtc cggatagtct tcttcggggg tgatggtgat
     7321 gcttggcgcg atcggcatgt agcggctgcg gcggctgatg cggcggctat ggctggagat
     7381 ttcggcaacg gtgtgtccgt ggataaagct gttgagttcg gcttcgagcg cgctttggat
     7441 gtcggggtag tcttcggggg cgtgctgcga gggaatttgc acgatgaagg tgtcgaggat
     7501 gtagtcgtgt tcggtgataa aggcgcgggc ggcgagaatg tcgaagccgt ggcggctgaa
     7561 gatacggcag agacgggcaa acaagcgcgg cccgttgggc atgaaaacca taacttgaaa
     7621 gctgtcgctt ttgaacagga tgcggctgcg gacgatgggg gtttcaaaat cgtggactag
     7681 gttggcggcg tgccacagga tttcgcggga ctggtggcgg gcgaagtagg cggaaccgag
     7741 cgcgttccat agttttttct gctgtttttc ggggacggcg gcgcgggtga gtaagtcggc
     7801 ggcttcctgc cggcggcggc cgaagagggt gtgcgggttg ccgccgttgc ctgtaaggta
     7861 gcgtccggcg gcatggaaga ggctttccag caggctggcg cgccaggcgt tccatagttt
     7921 gggattggtg ccgcgtatgt cggaaatggt caggaggtag agtgcgctga ggcgttcgtg
     7981 ggtttgcacg cgtttgcaga aggcatcgag tacgtcgggg tcttggatgt cttctttttg
     8041 ggcgacggtg gacataagaa gatgattttc gaccagccag gcgagcaggt cgctttcttc
     8101 tttggtcagg aagtggtcgg cggcaaattg gcgtgcgtcc gcgatgcctt gtatggcatg
     8161 gtcgccgccg cgtcctttgg cgatgtcatg gaaaaaggcg gcaaggtaga ggatgtcttg
     8221 tttttcaaag gactgcatca gtgcagaggc gtagggcagc tcgtggctgt gcatatccaa
     8281 ggcaaggcgg cggacgttgc ggacgacggt gaggatgtgg tcgtccacgg gatagatgtg
     8341 gaacaggtcg tgctggagca ggccgacgat tttttcccac gcgggcaggt agcgtcccaa
     8401 tacgccatag aggttgagga agcgcagggt ttgggtcagt ccgttgccgt tgcggaaaaa
     8461 gccggcgaag cggtggcggt tttcagaatt ttggtagaag ctgcggttga ttttgcgcgt
     8521 cgcgccccac catgcgcgca gggtttgcgg ttcgagcgcg gtaatatcgt tgcactgctg
     8581 catgatttcg acgattttga aaatgtgttc gggccgtctg aaaaaaatat cggtgtgccg
     8641 cgcggcgatt tggttgttga cttggatgta gtcgtcgtca atccgcaggg taacgcgcat
     8701 cggcgcggag gaaacgcggc ttcgcagcat aggcgtgagg atgccgctca gttgtttgac
     8761 ggttttgacc gcacggtaaa acacgcgcat cagttcttcg ctttggcggc ggaggttcaa
     8821 gccttcataa cccatgcttt cggcgacttg cggctgcaaa ttgaacagca ggcggtcttc
     8881 ggcgcgcttg gcgtttaaat gcagatggat gcggatgtgg gcgaggcggc ggtagccgtg
     8941 cgaaagcata ccggcttcgg cacgcgtcaa aatccgctgt ttgagcaggt cgggcaggtt
     9001 ggtcgccaag ccttgtgctt tcgctatcca aagcagggtg tggatgtcgc gcagaccgcc
     9061 cggacagctt ttgatgtttg gttccaatac cgcccctgag ccttgcgatt tggcgtggcg
     9121 gtgttccatc tccaccagtt ttgcctcgac aaacgccgcc acattgcgct gcgcgttcat
     9181 tttttccgct agtttatccg ccgtttggcg gttgccaaac aaaaacctag cctctaaaaa
     9241 cgctgtgtcc cccgtaatat cattgcgcac gctttcacat agttcatcaa cgctgccgct
     9301 ttttacagac ggtatcagtt tgcagtccca cagggtttgg acaaaccggg caatctgttc
     9361 ctgaatgccg tctgaaagcg gggcagggga gacaaccgcc aaatccacat ccgaacaggg
     9421 atacagttcg ccgcgtccga agccgcctac cgccatcagg cataacgcgc tgttttgaaa
     9481 atactctgcc cacaatgccg ccaacaaggt tttgagtgcc gccgtatatt ccctgaaaaa
     9541 taccgacacg cggttggctt tcaaataatg cgcttcggcg gcatcgcgct gctgtttgaa
     9601 ggtttccagt gctgaagaca ggtttgcagg catttttatt ctttcgattg gcgggaaaag
     9661 gggaggcgga tggttcggcg gtcaaatacc gctttcagac ggcatttgtc gggtatcggc
     9721 ggaattggtc atcaggttca accgttcctg cggcggcagc agcaactgat acgccgccac
     9781 gcccaaagcc gccgccattt tttcgatatt cgacagggcg atgttccagc gtttgcgttc
     9841 gactgccgac acataagtcc tgtccaaacc gcattgccgc gccaattctt cttgcgacca
     9901 acccttgttc acgcggaaaa gccgcatatt gtatgccaat accgcccgca aatcctgttc
     9961 gtcaggcagt tcggcaggca gagtcaattt gttgcccatc attatgtttc cggataaatg
    10021 gttaaagtta agcatttgct gttacggatt ttacttcaca taaaagccaa aaaaaggggt
    10081 ggcggcagcc acacccaaac acacacacat caagaggaaa gaggtaaaat caagacagat
    10141 tgggaaacag caaacaagca atctagattt catatcggta ttatcaaaca aaaaccctta
    10201 tcaggtattg attcaaatca atttagattt tattcgcaaa ccgaaaaaga aaaacggcat
    10261 atccgtttat acggatatgc cgtctgatgg tgcgggggac tgctgtattt ggcagtagtt
    10321 tgtgttttag tcctcttgcg ccgctgtttg caggtagttg ggcagaccga ttttgccgat
    10381 aagctcttgc tgggtctcca gccagtctat atgttcttcg ttggtgtctt tttgtttttc
    10441 caacaaatcg cggctgacgt aatcctgttg cgcttctgct gtggcgatgg cggcaagcag
    10501 ggcttcgtgt ttttcctgtt ctttggtcaa atcgcaggcg atgatttctt cggtggactc
    10561 gccaatcaga agcttgccca gttcttgcag gttcggcaga ccttcgagga acagaatgcg
    10621 ctcgatcaaa tcgtcggcag ctttcatttc aacgatggac tgtttgaaga aatgttcgcc
    10681 cagttcttca aagccccagt ttttcaaaat acgggcgtga aggaaatatt ggttgatggt
    10741 taccagcagc aagcctaagt ttttgttcag ctcgcggata accaaacggt cgcctttcat
    10801 acggactcct tttattccga atacggatta cagttggctt tggtagtagt tgccttcgcc
    10861 aatcagctcg atcaggcgca gctgctgttc cagccagtgg gcgtggtctt cttcggtatc
    10921 tttcagttgg gcgaccatca aatcgcggct gacatagtct tgcgcctctt cgcacagttt
    10981 aatgcctttt ttcaaagcgt cgcgcacttc gtattcggtt tgcaggtcgg ctttgaggca
    11041 ggaaaccacg tccgtgccga tattcagctc ggcgcgcgcc attttcggcg taccgcccag
    11101 catcaggatg cgtcggatga agtcttcggc gtgtgtggtt tcttcttcca tctcgtgatt
    11161 gagacgttca aaaagtttgg tgtagcccca ttcggagtag aggcgggagt ggataaagta
    11221 ttggtcgcgt gccgccagct cgccagacag caattcgttc atataatcaa caacagcttg
    11281 attgccttgc ataatatctc tctttccgta acttggtttt cggtatgtct gaacagcatc
    11341 tgtgtcgcta ttcgggatgc gtgcattgta tgcaaaagct gtcggcatga caaattttct
    11401 atttaaaata caaacaatta tcaaaagaaa tagggcgtat tccccgatgc tttccaaatc
    11461 agtatgctgt ttcttatcgg tttttttgct gctaaaacta agaatccatc tcatccataa
    11521 agaacattta cacttattaa ccatagcaaa aaacaaatag agcacggttt tttatcaaaa
    11581 tttataatga atcgttctca ttaaccgaca gattcttttg agattatcgg attttgggaa
    11641 taaattatgc cgtctgaggg gctttcagac ggcatgcctg cccggacaga cgctgcttgg
    11701 agcggctgtc ggtttgaagg tgtgtttaat gcgaagggca atctgtcgta tgatgtgttt
    11761 gcggcagggg ctttgaagaa gcccggtatt ttaggacgaa gcgcgcggct gccggggtca
    11821 atatccgcta tttattccaa accttattaa aacttaaatt taaaatcatg aagatagaaa
    11881 atatcgatat tatttcgccg gaactgttcc cgcaggaaac attcaatgaa accgaagcat
    11941 tcggcgcttt ggtatggttg tgggcagttt cgcctattta tcagcatgcc ggcgtacaag
    12001 aagccgccgt caatatcttg ccggtattga aaaacgggca atttgccttg ttcagcagca
    12061 acggacaccc cgtcgcctac tgcacttggg cttatttcga tgaagaaacc gaatggcaat
    12121 atctccaatc caatgatgtt ctgcgccact cggaaaactg gtgcagtgga aacagaatgt
    12181 ggcttatcaa ctggttcgca cctttcggcg acagccgtat gatgaaaaga attctggtgc
    12241 atctgtttcc gaaacgtgaa ataaggtggc tgtaccaccg ggggagtgaa aaaggaaaac
    12301 ggattatgag gtttccagcg ttgtcgaaac aataaaacaa ggctgcctga aagtttccct
    12361 tcaggcagcc ttgtttcagt tctctcgagg ccattcgtat tttttgtttt cgggataaat
    12421 gtcgtcacct acacgatacc aacctctttt tctcatgcag gcatctgctt tacttcctcc
    12481 tcccccacct attgggtcat agccgcactc tttccagtct tttttttcct tagcaaaaag
    12541 atcatctatt tgtttgctat attcttcata agaatatatg cgtaaattga tatttttact
    12601 catcattgcc ggatattctt gttctatacg ggaatacttc cagtataccg agtcatcggg
    12661 cggaggtttg aatcccccaa acgaacaggc agccaatccc atagccaaag agattgatac
    12721 gatatatttc attttgtttt tccttcttct cgaggccatt cgtatttttt gttttcggga
    12781 taagggtcga aacccttacg acaccagcct tttttcctca ggcaggcatc tgcttcagac
    12841 tttccgccgt ctattgggtc atagccgcac tcttgcatgt ctttcagttg ccttcgtctt
    12901 gctttcgatg gatattggtc aagtgccgcc gaggctgatc ccggataggg attggcgtaa
    12961 tttttcaatt cccaaaatga cgcggcgtcc cacggatttg gtttaaatcc cccaaacgaa
    13021 caggcagcca atcccatagc cagagagatt gatacgatat atttcatttt gtttttcctt
    13081 cttctcgagg ccattcgtat tttttgtttt cgggataaat gtcgttacct acacgatacc
    13141 aacctctttt tctcatgcag gcatctgctt cactcccacc gcccccacct attgggtcat
    13201 agccgcactc tttccagtct tttttttctc tagcaaaaga atcatgcagt tgtcttcgtc
    13261 tctcttcagg tgggtattgg tcaagtgtcg ctgaggttaa tcccggataa agtttggcgt
    13321 aatttgtcaa tctccaaaat gccgagtcat cgggcggagg tttgaatccc ccaaacgaac
    13381 aggcagccaa tcccatagcc agagagattg atacgatata tttcattttg tttttccttt
    13441 cggatgttgc agatcataaa tgcgtacagg acggtacata atttcgattt cgttggacac
    13501 tcctctttca aatacacctg tcggactttg ggtttgttta ttttcagggt ctcgataacc
    13561 atctgttaca cattgtttat tagccatacc gtagcaatta tgcggcgagg tgtaatcccc
    13621 tataatatcc cttatagcct gccattggct tttttgccgg gtattggttc cgactgttgc
    13681 cggatttctg ccaatcagca tgccgataag gtctaattcg tggttttcta tttggatgga
    13741 ctgacgggcg aaatctgccg ttctgggtgt cgttaccccc tgctgcagtt gaaacagcct
    13801 tttatctgcc cggacaacgt tggcggcagg gccgaccatt ttcagttcgg tattggataa
    13861 aattttctta tcccgattgg cttgggtatt caaggtattg agtacattgt cgaccaccag
    13921 ggttccacgg ctgtgcgagc cgacaaacag gccgttttta tccttcccgt agtcttccat
    13981 gatattgcct aaagccaagc ctgaattgct caagccgata acggtcttat tgcctatttt
    14041 tgccccttcg agcattttat gaaaggccgc aacggcgatt tcgggcaatt ttgaaaagcc
    14101 ccgcccattg gtttccggat tgtgcaggaa ataaacattt tcataggtgc gttcatatcg
    14161 gtttttctcg ggattgaaac gccccacata ttgttgggcg gcaaatttgg cggctgcttg
    14221 tatattattg aaaatgccgt tgacgccgac aacgattttt tcaacggttt tgccggtctc
    14281 cgggtcggtg taacgggcag ttttcagatt tttccgttcc tggtcggata cttcgcgcaa
    14341 ttcgtagata tttcccccaa tggctttata ggcttcccaa taatctttaa atgaggcaaa
    14401 ttcttttttc aactttactg agttgtcaaa ctgtttattt atatcctctt caagctgctt
    14461 atcttcaatc ggctcgccgt tttcatccat tttaaaggtc atcaggcggt gttctgcaat
    14521 aaactggctg cggtaggctt cgtctgcaat gccgccgatc agcgtctcat tgataaactc
    14581 tttggcaaca tccctattga gttcgacttc tttcagcaag ccttcgcggt ctgcttttgc
    14641 caatgcctta tgtgcggatg aagtgccttt ttcaataccg gcaatatttt tcctgccctg
    14701 cgcggaagca atttgccaat ctccctcact gatgacggag cgggtaatgg attggcggct
    14761 ttctgattct ttggcattgc ccaacagatt gcccaaaccc atttttgcca aggcgtattt
    14821 gtcgttgctg tgtatatcgt tttgcttcaa accgaatttc aagccgccgg cgttgttttg
    14881 atttaaattt gcctctttaa aggtttcccc tttttcaata cgctcacttc gatatttttc
    14941 gttcaactcg tcgtctttcg ccgaaacctg gtcaaaacgc atcaggctga ccggtttgcc
    15001 gccgatttta ccgatttctg cttctttttt ggtggaatac tgtccgctgg taggcttcgg
    15061 gctgtatgaa aaaccgccgc tcaagcccaa ggcggaagca gccgccgaag catggttttg
    15121 aatatcttta tgccagattt cgcttgtttt cagcaggttt tttgatttgt cggcatctga
    15181 aacaacagcg gcgccgacca atccggtttt gccgtttacg cgaatccgat agccgtctcc
    15241 tcctgcaaag ataccgcttt gctcgttgac ggatgcataa tccgagctgc ttttcgagcg
    15301 gttatagctg ccaccgacac taaagccgta gcctaccgta acttgggcgg aaacattttc
    15361 ctgtttgcct ttaaacacgg cggtatcctg caaactttcg atatgcagac tctctgccgt
    15421 tacgccaacg cctttgcctt taagctgccc gcctttgatg acggtatcgc caccgctttc
    15481 aatagcggtt tggctgtctt tgctgccgat atggctgttg cggtaggcgg tttcgtcgcc
    15541 gttgccgtaa cctttgccgt agtttgctcc ggctgtgaag ccgaaactga tgcctttgtt
    15601 gatggcgatg gcgactccgg cattaaagcc tgcggatttg ttttcgctgc gttcctgatg
    15661 cgtttggcgg gcggcttcaa tctgaacggc attttctgct ttgaggcgtg ttcctttgcc
    15721 gccgtacaca tcggagccgg taatcgtgat gcgggagtct ttgcctgcgc ctgaagcagt
    15781 cagggaaact ttgccgccgc cggtgatttt gccctcttgc acctgcgtgc ctttgatgcg
    15841 gctttcggag gtgttcttct gttcgccgta ggtaacggag acgctgatgc cctgaccggc
    15901 tgcttttttg ggatttcggg cggcattata gagtgccacg ccggaatcta ctcctttatt
    15961 caaggcgttg gcagcagcca tggcattgac ccggctgttt ttgcttttgc cgacggtttg
    16021 gactgctttt acggcgtcaa ccgcgcccat tacggtattc acaaccggaa cgctaatggc
    16081 gacggttacg cctttttgtt cgtaaacctg cttactctct tggctgtaac ggttttgtgc
    16141 ggcatcgatg ctgatttttc cggaggaaat gccgacatcg ccttggggcg aggatatggt
    16201 cgaaccggtt tgggtgtaat gttttcctgc cgaaatcagg gtattgccgt tcaggctgcc
    16261 gacgacgctt tctgtgtggc tgacggtctc ggatcggttg gtttgcgtgt cttttttgct
    16321 gcccgccgta aagccgatgc cgccgctgcc catcagtccg gatttttctt ttttgttcat
    16381 ttcggcactg cggctgcgtg tttcggctgc tttaaggacg atattgtttt ttgccgagag
    16441 aatggtatgg ttgtctgcaa tgatattgct gccagtaacg gtaatatcgc gtcctgaaac
    16501 cagaatgatt tctttgccgt ccagcgtgcc ggatacggct tgtccgtttt ggttcttgag
    16561 atggcgggtc atcttctgtt tgatgccgcc cccgcttcta ccggtgtatt tcagggcatc
    16621 ttcggtttcg gtatgggctt tgccggcttc gactttgata tcccgtccgg ctgccagttt
    16681 cagacggcct tgttcgctgc cgacctctgc tgcacggata cggatgtctc cttttgcatt
    16741 cagactgaga ttgccccggg tgcggatggt gctgccgact tcgttttgtt ctttgcgaat
    16801 cacatagttg tcggaatcaa agatagtgtt ctgattgcgg gaaatgcccg tcgtatccga
    16861 gcggatgtcg ccgccggcat tcagtacggt ttgaccgtct tcagattgat tggtcaattc
    16921 ggaagccgtc aggacgatat tgttgcctgc atccagcaag acgcttccat tctgcctgcc
    16981 ggtcagataa atgcctgcca cccggccgat attgcgtacc gagccttgct cattctgatt
    17041 gctgcgggtc tcgctgcggc tttctatatt gttactcgct ttgagcagca gggcgttttc
    17101 tgcgccgatg ctgccggtat tcgtaatgtc gctgcctgct gcggcgaaga tgtttttgcc
    17161 ctgcaaatca ccttgcagat ttttaatatt ctgtgcgttt aaaattagtg cttcgcgccc
    17221 ggcaattaag ccgccccggt tttcaatggc gccgctgccg atatcaacaa cgctgccgga
    17281 cagcaacgcc ccttgtccgt tcatatcttt ggggcgtgcg cggacataga ctttgggttt
    17341 caatacggtt tgagttgtcc cgtcgggcag ggtaacggtc tcgttttcca gccaaacaat
    17401 gtcggaagtc agacgggcaa cctgttcggc agacagggca atacccggag taagctgcaa
    17461 ttctttggct atggtaatgc cgttatccat caaagccttg aattgctctt cgtcattggt
    17521 ataaccgtcc aagcggcggt agcctgtcag cttggcgatt tgttcgttta ccagtttctg
    17581 ctcgtaatag ccgtcgccca aacgcttgtg gatatggttc gggtcttgtt gcagtgcggc
    17641 aagcatatag ccgctgccca gccatttgcg gtagtcggta aaggcagggt cggtttcaat
    17701 caaatagcct ttgttgtttg gcgcaatggc aaacaagctg ctgttcggca gagtaaatgt
    17761 aggatggatg ccgttttcag caacaacggg tacaacagtg ccgggtatat cagatgcctg
    17821 ttggggcgca tagcctttat aggctgaaat acccatacgg atggagctga cttcgggggc
    17881 cggttcgtag ggagaccgac tgtatcccgt agaatcccgc cctttcttgt gatgacggtg
    17941 gtaacggtgc aagtcccctt tatcggttat ggtttttgtt cccaaagttt catcattgtc
    18001 caatgcggat tcgggcgtac cgacgatcaa tctgccgccg gcaataatcc ggctgttatg
    18061 atttttcagc ttggcggcat ctaaaaccaa attgccgcca ctgatgattt gaccgggctt
    18121 ggattcggta attttgctct cggcggtgat tcgttcgtaa tcgtatttgt accagacaga
    18181 gtgggggctg ccgtccggag tccgcatatg taaagactca tcttcaaata tttcccagcc
    18241 caactctttt tgagaacctt ccggatagcg ttctgttctg ccttccgcct gatattcgat
    18301 acggtgttcg cgatgggttt cttccgtatg gaaacgcaga tgctcattgg tattctgcaa
    18361 atcttttgta gcgatacgga tattgcctga tgattcgatt gccgcactgc ggttgtgcag
    18421 tgatgtattt gctccttgca cctgtcggct tccattcaat gcagaaccga tatgaagatc
    18481 gccggagctg gacaataatg ccgcctctcg gttctcaatt tcccgcgctc caatatccaa
    18541 ccgctcccgc gctgcaatta ccgccgcttt ggtttcgccg ttgaccgttt cttcccggtt
    18601 caacaaggta tctgcctcaa ctgccacacg gctgccgtaa attctgcccg tgccggcatt
    18661 gtccgacttg gcttcggttt gcagcagcgt tataccgttg ctgttgatta aacccctgtt
    18721 ggtgatgccg tttttgccgt ttaacccggt gcggtttccg gattggattt taccggagac
    18781 agtgttgtcg atttgggcgg cggtcaggga tacggtatcg cctgcctgta tgatgcgggt
    18841 gtttttcaga cggccttggg tggagaacgt gagtgtgcgt ccggcttcaa tatcgcgttt
    18901 gccggcaaaa tcatcatgaa gagaaacgga aacatcgcgt gcggcggtta atatgccgtc
    18961 gttgtccagt gattttgcct gtaaggaaac atctttgccc gcaataatcg tgccgtctgt
    19021 gttattgata tgcaggctgc ttttgcctgt atcgcgaata tccagcaaac cggccgagcc
    19081 gattaagcct gcttgattat ttatgccgtc tgaaaccttc aatacactgc ctttgccgct
    19141 gcggataaag ccttggcggt tatcaacggt ttgagcggaa atggtgattt cggcagcatc
    19201 aatcgaaccg ctgttgctca atttgccgtc tgcgcttaac gtaacgccgc ctgttgcggc
    19261 aaaaatccga cccttgttgc ggattacggc gccgttgtcg gtgctgatta aagtgatttt
    19321 gtctgcgtac atcccaccca gtgtggcggt gtcgatggca acggtaggag taacagaatc
    19381 cgaagaagat ggcgcagaag ctgttttggc aagagagccg tcaaaatcca atttgttctt
    19441 acccgaaacc accttgacat ctttacccca aacgcccgca ttgatttcag cagcacgact
    19501 aaggatacgg gtgtaatcgg catcagaggt atccaaacct ttgcccccaa tcacgacttt
    19561 acccgaagaa acatcaaagc ccgtcagatt gccgttattc aaaacaggaa cgcccgaagt
    19621 cagcgtaacc gaagcggcat taatcaatcc gccgccattc acacggatgc ccgacggatt
    19681 ggcaacgact acttcggcgc gtttgccgcc gacttcgata taaccgttca acaacgaagg
    19741 attactgctg tcaatctggt tcacaattac ccgcgcttcg ccgcgtgcca gatggggatt
    19801 gccttgaatc catccaccga gttgcgtttg cgtattgctg cggctgttgt ttagtattac
    19861 gcctttttca tcaacatcga actgcttgaa tcggttaaca gaaacgcctt gggatgacgg
    19921 agtttgaata ttgacttgcg gcaaaccgtt tgctgtctga agaataacgg cttgttggtt
    19981 tttaggggcg gatttgtcgg caatgatgcc ggaagcaggg gcaggggaaa acgcagcaac
    20041 acccaaagcc aacatgacag aaaaggcagc catacggaaa ccgaaggctg cccgggcaga
    20101 agaaacagaa gcggcaccgg tcactcgaac cgaagccgcc tcactatcct gcatactctt
    20161 gccgtcacga tgaacattct ctgctacagc catcatacaa ctgcgtttct tgttgaagat
    20221 aaccttgtag catcgcttgt tcatgatggg ttttcttaaa tgaaatgtaa ggaatagtta
    20281 aggacaaaat ataggaaatt tgaattaaat tgtcaataaa aaactgacag cgtaccctga
    20341 taatcagatg ttgtacgcta attgtgagaa gtatgtcggg atgattattt tgaagttttc
    20401 tttttattca aaagagttac tatgaaagtt aaccgggcgt aaataggctt caataaacag
    20461 gttatcgtta tttttcaatt gggaaaaaat cttccctaac ccatccattc gccaaattat
    20521 tatgtgggaa ttcggcaatt tcaataacgg ctgttgatcc atatctgctc ggtcatttag
    20581 cgttataaag tcgcaaatag cgtcagaatt tacaagatta tcggattttg ggaataaatt
    20641 atgccgtctg aagggctttc agacggcata ggcggctaac acgggtgcgg cttgcgccaa
    20701 acggcgcggc aggcgcaggg aatcgctttt tactttggcg cggtgttccg ggtttaatga
    20761 tgcccgcaac cgccgtggct ttccctcaag gcttccgccg cctgttcgtc ggcgtggtag
    20821 ctggaacgta ccatcgcgcc gatggcggca ttgctgaagc ccagttcgta tgcttctttt
    20881 tcaaagattt tgaactgctc gggcgtaacg tagcgcagga cgggcaggtg tccgtctgaa
    20941 ggctggaggt actgtccgat ggtaatcatt tcgatattgt gcgcccgcat atcgcgcata
    21001 atttcacgca cgtcttcgtc tgtttcgccc aagccgacca tgatgccgga tttggtcggg
    21061 atgtgcggca tcatttcttt ataacgtttt aataagtcta aagaatgttg ataattggca
    21121 ccgggacggg cttttctgta caggctcgga tgggtttcta ggttgtggtt catcacgtcg
    21181 ggcggggttt cggcaaggat tttgagtgcg atgtccaagc gtcctcggaa gtcggggacg
    21241 aggatttcga ttttggtgtt cgggctggtt tcgcggatgg ctttgatgca gtcggcgaaa
    21301 tgctgtgcgc cgccgtcgcg caggtcgtcg cggtcgacgg aggtgatgac gacgtaacgc
    21361 aggttcatgg ctttgacgga ttcggcgagg tttctcggtt cgtcggggtc gagcatattg
    21421 ggccgaccgt gtcccacgtc gcagaacggg cagcggcggg tgcagatgtc acccataatc
    21481 atgaaggtcg ccgtgccttt gctgaagcat tcgccgatgt tggggcagga ggcttcctcg
    21541 caaacggtgt gcatcttttg ttcgcgcaaa atgtctttga tttcaaagaa tttgcgcgat
    21601 gggagtttgg cgcgtatcca ttcgggcttt ttcagttttt cctgaagggg gacgactttg
    21661 atggggatgc gcgcggtttt gtccgcgcct ctgagtttga tgccgcgttt ggggtcgtcg
    21721 gttttgattt cactcattgt tgtctgcttt cggtgtgaat tgtgtttcaa ggtgtgcggt
    21781 gagtttggcg gcgacttcgt ccggcgtggg gcagggttgg acaaaatccg cgatttgcgt
    21841 catttccata ccggcgtagc cgcaggggtt gatgtgggta aacgggctta aatccatatt
    21901 gacgttgagc gcaagcccgt gatagacgga gccgtttttg atacgcagcc ccagtgaggc
    21961 gattttgcgt tctccgacat aaacgccggg gcgtttgggg tctgccgccg cttcgatgcc
    22021 gtattctgcc aatgtggcga tgatgctgtt ttcaagcgcg gaaacgatgt ttctaacact
    22081 ggttttgcgc cgtttgaaat caatcatcgt ataaacgacc aattgcccgg gcccgtgata
    22141 ggtaatctgc ccgccccggt cgatttggac gacgggaatg tcgtcgcgaa tcagcaggtg
    22201 ttcgggtttt cccgccagtc cttgtgtgaa cacgggcggg tgttcgacga cccacagttc
    22261 gtcttcggtg tcggcattcc gtccggcatt aaaggttttc atcgcttcaa aagtcggcag
    22321 atattcgacc aaacctttgt gtatgatttt catctcaaag taccactttg accagttcgt
    22381 gcgaagtcag cgcacggtag atgttgtcca attgttcttg gttttcaacc tttacctgta
    22441 cggtggcgcc agtatagttg cctttgctgc tcggacgcgt ggtgatgtgg tgcgcctgcg
    22501 tgtcgggggc gtggaggcgg acggtgtcta aaaccgcctg ctcgaactcg ggatgcaccg
    22561 cgcccattac tttcaatggg aaggtgcagg gaaattcgat gagggatgtt ttgttttttt
    22621 gttcggtcat gatgtgctgc cttgtcgtgt acggtatgcc gtctgaaggc gggtttgcct
    22681 ttcagacggc atcggatgtg cgttatttta gcctaaaccg cgataacagg ctatcgggaa
    22741 ggcggaggct tttttgacgg cgcggcggtt ctgctatact ggcgcacaat attattttta
    22801 gaagggtggt ttttatgtat cggaggaaag ggcggggcat caagccgtgg atgggtgccg
    22861 gtgcggcgtt tgccgccttg gtctggctgg ttttcgcgct cggcgatact ttgactccgt
    22921 ttgcggttgc ggcggtgctg gcgtatgtat tggacccttt ggtcgaatgg ttgcagaaaa
    22981 agggtttgaa ccgtgcatcc gcttcgatgt ctgtgatggt gttttccttg attttgttgt
    23041 tggcattatt gttgattatc gtccctatgc tggtcgggca gttcaacaat ttggcatcgc
    23101 gcctgcccca attaatcggt tttatgcaga acacgctgct gccgtggttg aaaaatacaa
    23161 tcggcggata tgtggaaatc gatcaggcat ctattattgc gtggcttcag gcgcatacgg
    23221 gagagttgag caacgcgctt aaggcgtggt ttcccgtttt gatgaggcag ggcggcaata
    23281 ttgtcagcag tatcggcaac ctgctgctgc ttcccttgct gctttactat ttcctgctgg
    23341 attggcagcg gtggtcgtgc ggcattgcca aactggttcc gaggcgtttt gccggtgctt
    23401 atacgcgcat tacaggcaat ttgaacgagg tattgggcga atttttgcgc gggcagcttc
    23461 tggtaatgct gattatgggc ttggtttacg gtttgggatt ggtgctggtc gggctggatt
    23521 cgggatttgc catcggtatg cttgccggta ttttggtgtt tgtcccttat ctcggggcgt
    23581 ttacgggatt gctgcttgcc accgtcgccg ccttgctcca gttcggttcg tggaacggca
    23641 tcctatcggt ttgggcggtt tttgccgtag gacagtttct cgaaagtttt ttcattacgc
    23701 cgaaaatcgt gggagaccgt atcgggctgt cgccgttttg ggttatcttt tcgctgatgg
    23761 cgttcgggca gctgatgggc tttgtcggaa tgttggcggg attgcctttg gccgccgtaa
    23821 ccttggtctt gcttcgcgag ggcgtgcaga aatattttgc cggcagtttt taccggggca
    23881 ggtaggcggt tcc
//
LOCUS       DAWHRS010000011        21855 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWHRS010000011 DAWHRS010000000
VERSION     DAWHRS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.011"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1166)
                     /locus_tag="WIB82_000313"
     CDS             complement(<1..1166)
                     /locus_tag="WIB82_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ3841674.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
     gene            complement(1223..3748)
                     /gene="ligA"
                     /locus_tag="WIB82_000314"
     CDS             complement(1223..3748)
                     /gene="ligA"
                     /locus_tag="WIB82_000314"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ3841675.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARRQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTVVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(3896..5182)
                     /locus_tag="WIB82_000315"
     CDS             complement(3896..5182)
                     /locus_tag="WIB82_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ3841676.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKLQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(5376..5948)
                     /gene="ampD"
                     /locus_tag="WIB82_000316"
     CDS             complement(5376..5948)
                     /gene="ampD"
                     /locus_tag="WIB82_000316"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ3841677.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            6032..7027
                     /gene="mltG"
                     /locus_tag="WIB82_000317"
     CDS             6032..7027
                     /gene="mltG"
                     /locus_tag="WIB82_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ3841678.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7087..7707
                     /locus_tag="WIB82_000318"
     CDS             7087..7707
                     /locus_tag="WIB82_000318"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ3841679.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLDRAAACPERYAVIDSNLGLDEVRNSIEKVLDRHFGC"
     gene            7929..9209
                     /locus_tag="WIB82_000319"
     CDS             7929..9209
                     /locus_tag="WIB82_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ3841680.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            9445..9716
                     /locus_tag="WIB82_000320"
                     /pseudo
     CDS             9445..9716
                     /locus_tag="WIB82_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9756..10790
                     /locus_tag="WIB82_000321"
     CDS             9756..10790
                     /locus_tag="WIB82_000321"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ3841681.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAASRAEDAGDEPLLLFSKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            10996..11577
                     /locus_tag="WIB82_000322"
     CDS             10996..11577
                     /locus_tag="WIB82_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ3841682.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            11646..11828
                     /locus_tag="WIB82_000323"
     CDS             11646..11828
                     /locus_tag="WIB82_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ3841683.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            11825..12586
                     /gene="kdsB"
                     /locus_tag="WIB82_000324"
     CDS             11825..12586
                     /gene="kdsB"
                     /locus_tag="WIB82_000324"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ3841684.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            12609..12926
                     /locus_tag="WIB82_000325"
                     /pseudo
     CDS             12609..12926
                     /locus_tag="WIB82_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            13031..13228
                     /locus_tag="WIB82_000326"
     CDS             13031..13228
                     /locus_tag="WIB82_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841685.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            13385..13477
                     /locus_tag="WIB82_000327"
     tRNA            13385..13477
                     /locus_tag="WIB82_000327"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13419..13421,aa:Ser,seq:gct)
     gene            13780..14565
                     /locus_tag="WIB82_000328"
     CDS             13780..14565
                     /locus_tag="WIB82_000328"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ3841686.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            14602..15474
                     /locus_tag="WIB82_000329"
     CDS             14602..15474
                     /locus_tag="WIB82_000329"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ3841687.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPIDPLKFKDSKKYPDRLGAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(15534..16145)
                     /locus_tag="WIB82_000330"
     CDS             complement(15534..16145)
                     /locus_tag="WIB82_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ3841688.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHNK"
     gene            16254..16478
                     /locus_tag="WIB82_000331"
     CDS             16254..16478
                     /locus_tag="WIB82_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ3841689.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            16479..17084
                     /locus_tag="WIB82_000332"
     CDS             16479..17084
                     /locus_tag="WIB82_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841690.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            17144..18178
                     /gene="pyrC"
                     /locus_tag="WIB82_000333"
     CDS             17144..18178
                     /gene="pyrC"
                     /locus_tag="WIB82_000333"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ3841691.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(18546..18971)
                     /gene="nusB"
                     /locus_tag="WIB82_000334"
     CDS             complement(18546..18971)
                     /gene="nusB"
                     /locus_tag="WIB82_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ3841692.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(19049..19525)
                     /locus_tag="WIB82_000335"
     CDS             complement(19049..19525)
                     /locus_tag="WIB82_000335"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ3841693.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(19573..19896)
                     /locus_tag="WIB82_000336"
     CDS             complement(19573..19896)
                     /locus_tag="WIB82_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ3841694.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            20063..20782
                     /gene="rnc"
                     /locus_tag="WIB82_000337"
     CDS             20063..20782
                     /gene="rnc"
                     /locus_tag="WIB82_000337"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ3841695.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            20829..21752
                     /gene="era"
                     /locus_tag="WIB82_000338"
     CDS             20829..21752
                     /gene="era"
                     /locus_tag="WIB82_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ3841696.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         4830 a         5371 c         6491 g         5163 t
ORIGIN      
        1 cactgcagca aatcgtttaa aaacaagcgt cctttttcgg tcgggcggaa tacggcaggg
       61 tcggtttcca gcaggccttt ttgccttgcc gtttcgattt gcgccatgat tttggcactc
      121 ggtacgcccg tgcgctcctg caacatcgcg gcgggtacgc cgtcggtcag gcgcagggcg
      181 ttcatcatga attcgaacgg caaatcttcg gcagcgacgg ttttgcgttc gacggcttca
      241 ctcggttggc tttgcattaa ggcgaggtag tcgttggggt ggcggcggcg gacggtgcgc
      301 tcgatgcggt cgggatagga aattttgccg tgcgcgcccg cgcctatgcc taaataatcg
      361 ccgaactgcc agtagttcaa attgtggcgg cactgcatgg ctggtttcgc aaaagccgat
      421 gtttcgtagt ggacaaaacc cgcgccttcc agcgcgccgt gtaccgcgtc ttcgatgtcg
      481 agggcggctt cgtcttgcgg caaacctttc ggcggcgtat gaccgaacgg cgtgttcggt
      541 tccatcgtca gatgatacgc gctgatgtgg gtcgcgcccg tagcgatagc ggtttgtacg
      601 tcgtccaatg ccgtctgaac ggtttggttc ggcagggcat acatcaagtc gatattgact
      661 ttatcaaata atttcaaggc ggtatcgata gcggttaagg cttccttacc gttgtggacg
      721 cgccccagcc ttgagagcat atcgtcgttg aaactctgta cgccgataga aagccgcgta
      781 atacccgcgt ctttaaatcc ttgaaacttc tcgatttcaa atgtccccgg attggcttcc
      841 aaggtaattt ccgcttccgg ctgcaagcgc aacagcgaac gcacgccgct taacaaacgg
      901 tcaatcgatt ccgcctgaaa caggctgggc gtaccgccgc cgaaaaagat cgtttccacc
      961 ggcctgcccc aaatattggg caattcaagc tgcaaatcgg tcagcagcgc gtcgatatag
     1021 gcggcttcgg gcaatccgtt tttcaggctg tgggaattga agtcgcaata cgggcatttt
     1081 ttgatgcacc acgggatgtg gatgtaaagc gacaggggcg gcagggcggt gagtcgggtg
     1141 cggtttggaa aggaaatggt gtgcatggtg tggttcggaa aagtgggcaa tgccgtctga
     1201 aggcggttca gacggcatgg gttcagccga gcagggtaag cagttcggct tcgctgagga
     1261 cggaaacgcc caaggcattg gctttttcca gcttgctgcc cgcggcttct ccggcgacga
     1321 cgtaatcggt ttttttggac acgctgccgg aaactttgcc gcctgcggct tcgattaggg
     1381 attgggcttg gtcgcgtttg agggtgggca gtgtgccagt taacacgaag gttttgcccg
     1441 ctacggcttt attgatgccg tctgaacctt gcgccgcctc gtcttcagac ggcatttgcg
     1501 cgaagaaggt tttcaggttt tcgagtaggg cggcgttttg tggttcgctg cgccatgctt
     1561 gccagtcggc ggggagggat ttgtcggttt gcagaccctc tatacttttg ccggcgagtt
     1621 cccataaggc ttgggctttg ttttcgctaa ttttgaaacc gggcaggcgg gcaatccagc
     1681 gttgcggttc ggcatattgt gcggcaggca ggctgacggc ttgggcttgc ggggcaacgc
     1741 ctgcggcgag cagctcgtct atcatcgcct gctgttcggc ttgggcgaag aagtgggcaa
     1801 tggaacgcgc cactaccgta ccgatgtcgg gcaggcaggc gaggacgggt tcgggggcgc
     1861 ggcggacgcg ttccaatgtg ccgaatgcct gtgccagcgt tttggcggtg cgttcgccga
     1921 cgtggcggat gccgagcgcg aacaggaagc gcgcgagttc gggcgttttg ccggattcta
     1981 tgcctgcgag gatgttttcc gcccacttgg tcggttgttt tttatgtttg cccgacgcac
     2041 cgaccgaact gccttcagac ggcatttgat ccgattcggc aacggttttg tccgccgttt
     2101 ccttcatttt ttgcagggtc ggaatatcga ggcggtagag gtcggcgaag tggcggacga
     2161 ggtcttgggc gacaagctgt tcgatttgtt tttcacccaa gccgtcgatg tccatggctt
     2221 tgcgcgaggc gaagtggatt aagccttgcg cgcgttgtgc ctgacaaagc ataccgccgc
     2281 tgcatcgggc gacggcttcg ccttcttcgc gttcgatttc gctgcggcag atggggcaat
     2341 gggtcggcag gcggtagggc ttgtagagcg gaacggattg ggtttggttt gcagccggtg
     2401 cttcggcaaa caaatcgtcc tgccgatgcc cgatgccgtc tgaaacagca acggcggttt
     2461 cctgcatcgg gcggcgttca aaaatcacac gcacgacttc gggaatcacg tctccggcgc
     2521 ggcgcacgac gacggtatcg ccgacgcgca cgtcttttcg ggaaacttcg tcctgattgt
     2581 gcagggtggc gttggtaacg gttacgccgc cgacgaatac gggttgtaat cgggcaaccg
     2641 gcgttaccgc gcccgtcctg ccgatttgca cgtcaatcgc ttcgacgacg gtcagggctt
     2701 cttcggcagg gaacttgtgg gcaatcgccc agcgcggcgc gcgggagatg aagccgagtt
     2761 cgcgctgttg cgccaggctg ttgactttaa ccaccatgcc gtcgatttcg tagggcagtt
     2821 cggggcgttt ttgctgcatg tgttcgtaaa acgccaatac ttcgtcgata tttttgaaac
     2881 agccgaaatt gccattgggc agactgaagc cgagtgcttg gaaataggcg agttcttgga
     2941 tgtgttcttc cgcgacgaaa ccgccttgcc ggcgggcgac ggagtagggg aaaaagtgca
     3001 gtttgcgttg cgcagtgatg cgcgaatcga gttggcgtag gctgccggcg gcggcgttgc
     3061 gcggattggc aaagggtttt tgcccgtttt cggcttgtct tttattgagg gcggcaaaat
     3121 cggctttttg catcagtact tcgccgcgta cttcgatgag ttcgggcgta ttttcgccgt
     3181 gcagccgcaa ggggatgttg gatacggttt tgatgttttg ggtaacgtct tcgcccgtcg
     3241 tgccgtcgcc gcgcgttgcc gcctgcacca atacgccgtc gcggtagagc aggctgatgg
     3301 cgaggccgtc gaatttgggt tcgataacgt attcgggatt gccgccgtcc aagccgtcgc
     3361 gcacgcgttg gtcgaaggcg tacatttcgg catggtcgaa cacgccgttt tcatcttgcg
     3421 gggaaaaagc gttggtcagc gacagcatcg gcacttcgtg gcgtacttcg gcaaatcccg
     3481 ccaaaggctc gccgccgacg cgctgggtcg ggctgtcggg cagtttgagc tcgggatggt
     3541 ttaactccaa cgcttcgagt tcgcggaaca atttgtcgta ttcggcatcg ggtacgctgg
     3601 gcgcgtcgag ggtgtagtat tcgtaggcgt agcggttgag gaggtcggtg aggcggcaga
     3661 tgtgttgtgc ggcaaattgt tttatatcac tatcagacgg tttaagaaga ttggtaaagt
     3721 tagtgttatg ttttgagttt ggattcatga gagaaggttt tcagacgacc tttgtctgat
     3781 acgggatgaa acgggcaaag gtcgtctgaa aaatgatagg ttgaaaacag ctgaatttta
     3841 cccgaaaaaa agcggatatg ccgtaacgac atatccgctt tgattgcatt cgattttagg
     3901 agaacaggcg caatgcggtt ttgccgcccg gttcgatacc gactttgagc atctcggact
     3961 gacgcgccaa tacataagtg cgcacgtctt tgagccattg ggtcgaaact tcttccattt
     4021 tgtcgttgac cagattcagg ttcaactggc cggacaggcg taccgccaaa tccataaaca
     4081 aatcgtcgaa ggttttttcg cctgccggag agtgcgggat gtcgagcagc atactgaagc
     4141 ctttgtagga ctggttgtcc aaaagggcgt tggtaaacgg ctcgttgttg agcgagcaga
     4201 tggagaacat ggtcgagccc gacgtgtcgg tatagtggaa cgcgccgtcg tcttccaaaa
     4261 cgaaacccac gcccgttacg gcggaacgca gttctacgcc gctgatgctg gtcggggaaa
     4321 ccaaatggat ggcgatggtc tggtcgacgc gcgcgcagaa tgcgtccagt gcggaagcca
     4381 cttcgataaa ggcggcaagg tcggtgtgca gcgtctgacc gcccatgctt tgtgcgaatg
     4441 cgtccacctg gcggttgaat gcggagagtt cttcctgcga ggcaagtccg ttgcggctga
     4501 ctgcctgaat acccacgata aatgcctgat agcggatgcc cgggatgggt tcggcaatct
     4561 ggaaatggtc gtccatggtg cagccgacaa tctggtagcg gcagcggttg gaaaggcgtg
     4621 gcagtgcgtg cagttctttg gcttcggtca gcgcgatata ggagatgaag tcgaagcgca
     4681 cgtcaaacca gggtaattcg acttttgaca gttctttgag cgtaatcagc ggttttgcag
     4741 gtgtttgcgg aacgggtgca ggttttgccg gcgcgtcggc aggtttcggt gcggaatgtc
     4801 cggtttgggg ttcggaaacg gtgtgggcgg agttgccgat aatgccgctt tcttccaagg
     4861 cggtttcgat ttcggttttg aacggggagg cttttgcctg tttctgcttg gcgatgtaga
     4921 cggcatcctg ttcttgcagg ttgcgcatag cagggtcttg gagttttgcc gtttttttga
     4981 ccgccggttg gggtttcggc atcatgactg acccgccgga cggtttgccg tcgcggacat
     5041 ggctggtttt gctgttgagc agggcatctt tgtcggagtg tccgaactgg tcgcgcactt
     5101 ttttgcggta ttggttttcc tgatacatat tgtaggcgac aacggcgagg acggcggcga
     5161 ggaacagtac gatgtaaatc atggcaatca ctttaaattt cgggatgcag gatgcgcgaa
     5221 gtgcgggtac tgcggttaaa tcgggcttgc gtttccggca gtctgatgga acggccggtt
     5281 ataacgtttg aattataacg aaaattgcag ggtctgacag cagtgtgtcg aaataagcgg
     5341 aaattttccg aaatgccgtc taaaatctgt ggttttcaga cggcatttct gtccacggga
     5401 aaccctttcc cccgtatccg ccgccagtcg aaaaaatgac cggggtcggt tttgcggccg
     5461 ggcgcgatgt cttggtgccc cgttaccgcc gtgatggggt agcggcggca gattgcgtcc
     5521 aacaaggctt cgagcgaacg gtattgcgct tcggtaaagg gttcgaaatc gcagccttcc
     5581 agttcgatgc cgatggaaaa tgcgttgcat ttttcccgtc cgcgaaacga ggatacgccg
     5641 gcatggtatg ccatattgtc gcaggaaacg aactgtaccg tttctccgtc gcgtttgatt
     5701 aagaaatggc tggatacgcg caaagtgtgt atcaggctga agaacggatg tccgtcgggg
     5761 tcgagccggt tggcaaacag cttttccacc gcatctgtgc cgtattcgaa cggcggcagc
     5821 gaaatgttgt gcaacacgat cagggaaacc gtttcccccg tttccctcgg gctgaaattg
     5881 ggcgacgggg tatggcgtat gctttgaagc cagccgtttt gccagtgtgc ttcggcgtga
     5941 ttgtccatga tgttcttcct gtccggcggg taatttgggt tatactgtcg cccgaatttt
     6001 aagacgtatt ccgaatgctg ggaatcctac catgttgaga aaattgttga aatggtctgc
     6061 cgtttttttg accgtatcgg cagccgtttt cgccgcgctg cttttcgtcc ctaaagacaa
     6121 cggcagggca tacaggatta aaattgccaa aaaccagggt atttcgtcgg tcggcaggaa
     6181 acttgccgaa gaccgcatcg tgttcagcag gcatgttttg acggcggcgg cctacgtttt
     6241 gggtgtgcac aacaggctgc atacggggac gtacagactg ccttcggaag tgtctgcttg
     6301 ggatatcttg cagaaaatgc gcggcggcag gccggattcc gttaccgtgc agattatcga
     6361 aggttcgcgt ttttcgcata tgaggaaagt catcgacgca acgcccgaca tcgaacacga
     6421 caccaaaggc tggagcaatg aaaaactgat ggcggaagtt gcccctgatg ccttcagcgg
     6481 caatcctgaa gggcagtttt tccccgacag ctacgaaatc gatgcgggcg gcagcgattt
     6541 acggatttac caaatcgcct acaaggcgat gcaacgccga ctgaatgagg catgggaaag
     6601 caggcaggac gggctgcctt ataaaaaccc ttatgaaatg ctgattatgg cgagcctgat
     6661 cgaaaaggaa acagggcatg aagccgaccg cgaccatgtc gcttccgtct tcgtcaaccg
     6721 cctgaaaatc ggtatgcgtc tgcaaaccga cccgtccgtg atttacggca tgggtgcggc
     6781 atacaagggc aaaatccgta aagccgacct gcgccgcgac acgccgtaca acacctacac
     6841 gcgcggcggt ctgccgccaa ccccgatcgc gctgcccggc aaggcggcac tcgatgccgc
     6901 cgcccatccg tccggtgaaa aatacctgta tttcgtgtcc aaaatggacg gtacgggctt
     6961 gagccagttc agccatgatt tgaccgaaca caacgccgcc gttcgcaaat atattttgaa
     7021 aaaataaacc atgccgtctg aaaagtttgt gttttcggac ggcataccct taccggaact
     7081 gcaagcatga aaccgcaatt catcactttg gacggcatag acggtgccgg caaatccacc
     7141 aaccttgccg tcatcaaggc atggtttgaa cggagggggc tgcccgtgct gttcacgcgc
     7201 gaaccgggcg gaacgccggt cggtgaggcc ttgcgcgaaa tcctgctcaa ccctgaaacc
     7261 aaagccggtt tgcgtgcgga aacactgatg atgttcgccg ccagaatgca gcatattgaa
     7321 gacgtgatat tgcctgcact ttcagacggc atccatgtcg tgtccgaccg ttttaccgat
     7381 gcgaccttcg cctatcaggg cggcgggcgg ggaatgccgt ctgaagacat tgaaattttg
     7441 gaacattggg tgcagggcgg tttgcgcccc gatttgaccc tgctgttgga tgtgccgctg
     7501 gaagtgtcga tggcgcgtat cgggcagacg cgcgagaaag accggttcga gcaggaacag
     7561 gcagatttct tcatgcgtgt gcgcagcgtt tatctcgacc gagccgccgc ctgtccggaa
     7621 cgttacgccg ttatcgacag taacctcggc ttggatgaag tcagaaacag catagaaaaa
     7681 gtgttggaca gacatttcgg ctgttgatgc ggcaaatatt gaaacaagcg catccgcccg
     7741 cgccgaaaat caaacggcag tgccgcaggt gaaaatggcg gtatgcgcca aactttcggc
     7801 atgatagaat tacgctcggt tacaaggcag gatgcgtcgg caatattaac gaaccgcccg
     7861 taacatgatg acccgaaagc gtttcggaca gtccgattca aatctttttc tcgcaacagg
     7921 attgacacat ggaaaactca ttgaaagaag ccgccctcaa gttccacgaa ttacccgttc
     7981 cgggcaaaat ttccgttacc ccgaccaaat ctctggcgac cgacaaagat ttggcgttgg
     8041 cgtactctcc gggcgtagcc gctccttgta tggaaatcca tgccgatccg caaaatgcct
     8101 acaaatacac cgccaaaggc aacttggtcg ctgtcatttc caacggtacg gccgttttgg
     8161 gcttgggcga catcggcgcg ctggcgggca aacccgtgat ggaaggcaaa ggcgtattgt
     8221 tcaaaaaatt cgccggtgtg gacgtgttcg acatcgaaat cgatgaaaaa gacccgcaaa
     8281 aactcgtgga catcatcgcc gctttagagc cgaccttcgg cggcatcaac ctcgaagaca
     8341 tcaaagcacc cgagtgtttc tacatcgaac gcgaattacg caaacgctgc aaaatccccg
     8401 tattccacga cgaccagcac ggcacggcca tcattaccgc cgccgccgta ttgaacgctc
     8461 tgcgttttac cggccgtaaa atcgaagaag cgaccttggt ttgctctggt gcgggtgcgg
     8521 ccgcgattgc ctgcttgaac caattgctgg atttgggctt gaaacgcgaa aacgtaaccg
     8581 tttgcgactc caaaggcgtg atttaccaaa cccgcgaaga caaagatcgc atggacgaat
     8641 ccaaaaaatt ctacgccgtt gaagacaatg gccagcgcgt acttgccgat gccgtcaaag
     8701 gtaaagacat cttcttgggc ctctccggcg cgaacctgct gacccccgaa atgttgaaca
     8761 cgatgaacga aaaacccatc gtgttcgcca tggccaaccc gaatccggaa atcctgccgc
     8821 cgctggcaaa agaaacccgt ccggacgtgg ttatcggtac cggccgttcc gacttcccga
     8881 accaagtcaa caatgtattg tgcttcccgt tcatcttccg cggcgcgttg gatgtcggcg
     8941 cgaccaccat caacgaagaa atgaaacgcg cctgcgtgta tgctttggcg gatttggcga
     9001 tggaagaagt aaccgaagaa gtggttgccg cttacggtaa gaaatttgaa ttcggcgcgg
     9061 aatacctgat tccgactccg ttcgattccc gcctgctgcc gcgcgttgct acggctgccg
     9121 ccaaagcagc gatggaaagc ggagtggcaa cccgtccgat tgcagatttg gaagcttacg
     9181 ctgccaagct gagcgaatgg aagctgtaag ccgtttgcgg tttaaaatgc cgtctgaact
     9241 gttttcaggc ggcattttgc tgtcagattg atatagtgga ttaacaaaaa tcaggacaaa
     9301 gcgacgaagc cgcagacagt acaaatagta cggaaccgat tcacttggtg tttcagcacc
     9361 ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc
     9421 actataaatg aaagatactg gaaaatgaaa gagatgaaac ctgtccgtta tcatattggc
     9481 gatatgcccg agacttcaaa acaaaccgcc tcccgtcatg acgacagggc agtgggtgtt
     9541 gacgatgatt gttttcatga ttcctttggt caatttttgt ttgggtgttc ggcagaggca
     9601 acccgaaccg cgccaatttc tgtaaagcgc agttgcttat ttacctgatt ggttcgctta
     9661 tcggtttggt cttcgcgttg tttataggtg ggtctgtatc aggtacgcat gattaatgcc
     9721 ccgggctgat tttgcttcga ggatttgtat cgaatatgcc gaattgtttc aaatttcata
     9781 ccgttatcga acggcattgg caaaaacctt atccggtttt gtcttttctg cttaagccgc
     9841 tctccgggct gtttgccaaa attgcggcaa aacggcggac ggatttttta tcgggaaaac
     9901 ggcaaagcga aaagctgccc gtgcctgtgg tcgtggtcgg caacatccac gcgggcggga
     9961 cggggaaaac gccgattgtt gccgcgctgg tgtcgggttt gcaggaaaag ggcgtcaagg
    10021 tcggcatcat cagccggggc tacgggcgta agagcaaggc ggttcatgta ttgaatgccg
    10081 caagccgagc ggaagatgcg ggcgacgagc ctttgctgct gttcagcaaa accggtgcgc
    10141 cgacggcggt gggcagcagc cgtgcagagg caggcagggc gttgctggcg gcgcatcccg
    10201 acatcggact gattgtggcg gacgacggtt tgcagcatta cgccctgcgg cgagatgtgg
    10261 aaatcgcggt gtttccggcg gcggatacgg ggcgcacgga tttggattta ctgcccaacg
    10321 gcagtttgcg cgaacctttg ttgcggctgg attcggtgga tgcggtcgtc gtcagcggcg
    10381 gcaaggcgga tgcgctgttt aggccgtctg aaaatatgtt tcacagccgt atcgaagcgg
    10441 gacggattta ccgtttgaac aatccgtccg aaatactgga cacaggccgt ctgaaaaatc
    10501 aaaccgtcgt cgccgtggca ggtattgcca agccggcgcg ttttttcgat tcgttgcgga
    10561 atatgggcat taccgtgaag cgaaccgtcg cgctgcccga ccacgccgac atttcggcgg
    10621 cagatttgcc cgatgcggac gcggtcatta ttacggagaa agatgcggtc aaattttcag
    10681 acggcatttg caccgataat gtttgggtgt tgcccgtttg tgcgataatc gaacctgatt
    10741 tggcggcgtt tgtgttggag cggttggaag atgtaccgaa ggccgtctga aagcacggtt
    10801 tgggcggagt gattacggat ttgaataaga acgcctcgcg ccgtcattcc cgcgcaggcg
    10861 ggaatctaag tctcgaattt tcaggaatgc ctaggaggct tcagaaatcc caaatctccg
    10921 gatttccact tggacaggaa tgagaaaatc ggtcgtattt tttatctgca ttaatcattc
    10981 gttaaagtat tgaatattaa actgaaaacc ttgttattgc ccttcgccac gctggcattg
    11041 tgcaccaacg cttttgccgc cccgcctagc gacgcgtcgt tggcgcgttg gctggatacg
    11101 cagaattttg accgggatat agaaaaaaat atgattgagg gctttaatgc cggatttaaa
    11161 ccgtatgcgg acaaagccct tgccgaaatg ccggaagcga aaaaagatca ggcggcagaa
    11221 gcctttaacc gttatcgtga gaatgttttg aaagatttga ttacgcccga agtgaaacag
    11281 gctgtccgta atactttatt gaagaatgcc cgtgagatat acacgcaaga agaaattgac
    11341 ggcatgattg ccttttacgg ttcgcctgtc ggtcagtccg tcgttgccaa aaatccgcgc
    11401 ttaatcaaga aatcgatgag tgaaatagca gtatcttgga ctgcattgtc agggaaaatc
    11461 gcgcgacatc atctgcccgc gtttacggaa gagttgcggc gcatcatctg cggcggtaaa
    11521 aatcccgatg cgggctgtaa acaagccgga caggttggga aaaggcatca gaaataaatg
    11581 atagccgtct gaaatattga agagggcatc cgattgattg aaccatcaaa cccgaaagca
    11641 accctatgga aaaaaaattc ttagacatcc tcgtctgccc cgttaccaaa ggcaggctgg
    11701 aatatcatca ggacaaacag gaattgtgga gccgtcaggc gaagcttgcc tatccgatta
    11761 aagacggcat tccctatatg ctggaaaacg aagcgcgtcc gttgggcgaa gaggaactcg
    11821 aagcatgacc gaattcgtcg tattgattcc ggcgcggctg gattcgtcgc gcctgcccgg
    11881 aaaagccttg gcggacatcc acggcaaacc gatggtcgtg cgcgttgccg aacaggcggc
    11941 aaaaagtaaa gctgtgcgcg tcgtcgttgc caccgaccat cccgatattc agacggcctg
    12001 tcaggcgcac ggcgtcgaag tcgtcatgac ttcaaaccgg cacgaaagcg gcacgacgcg
    12061 ccttgccgaa gcctctgccg cgctgaagct gccgccgcat ctggtggttg tcaacgtaca
    12121 gggcgacgag ccgctgattg cacctgaact catcgaccgc accgccgaag tactcgtcga
    12181 aaacaacgtc caaatggcga ccgccgccca cgaattgcac gatttcgacg aattgatgaa
    12241 tcccaatgcc gtcaaagttg tcctcgacaa aaaccgcaac gccatctact tcagccgcgc
    12301 cccgattccc tatccgcgcg atgcgatgcg tgcaggaaaa cgcgaaatgc cgtttgaaac
    12361 cgccgtcctg cgccatatcg gcatctacgc ctaccgcgtc ggcttcctgc aacgctacac
    12421 cgaaatgagc gtttcgccgc tggaaaccat tgaatcgctg gagcaactgc gtgtcctgtg
    12481 gcacggctac ccgattgccg tcgaaaccgc caaagaagct cctgccgccg gcgtagatac
    12541 gcaggaggat ttggacagga ttcgcgctgt atttcagact atataaaata gattcaaagg
    12601 gaaaagatat gcagcaacat attgaaaaat ggcaacactt gagccgggaa gaacagaaaa
    12661 tccttgctga agtatggggt ctcgtgcaaa acgacgatca ggaggttcac tatgaaatgc
    12721 tcaaattgaa cgcacccgac gaagccagtg gcgaattttg gttcagaatg gcagaaacac
    12781 tcagcaccct gccgcccaac cgttccctcg accttagaat gaacggcggc aggctggcga
    12841 ccgccgtatc catcctttcc gtcatgattg aagacaatcc cgacataccg cagctttggg
    12901 cgcaaaaaat taccgcgctc aattatagtg gattaaattt aaaccagtac ggcgttgcct
    12961 cgccttgtcg tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
    13021 tccactatat ttggcacacg gacacaaagc ccgtgccgac ggtttggcac aacagcccga
    13081 caaagcggca gaagccaacg aggaggaata cctgaccaaa gccctgtcgc aaaacctact
    13141 gtcaacattg gatgccgcgc ttgcacgttt tcctgaagac gcgtggtttc aggaaatcaa
    13201 acaggatgca caaaagcatt ttgcttgagg atgtggcagt caggaatatt tccattcagg
    13261 aagaaaagaa gtgcctgatt gggtataatc agggtaaatc ttattttatt tcaaaagatt
    13321 aatatttgct ttctgttttt ccttgacggt atcggaaaag ttgattatag ttacagcttc
    13381 cttaggagta atggctgaga ggctgaaggc acttccctgc taaggaagca tgtggggtca
    13441 acctgcatcg agggttcgaa tccctcttac tccgccaaga tgagaagacg ctgtgtttta
    13501 cagcgtcttt ttttacgcgc tttgttgcgg aagtgccgag ggctgtctgc cgtgcgccgc
    13561 ctggatttgc aatgtgcccg cccgtgcaaa atccgatttg accaacctga agggcaggtt
    13621 tcggaagagc ggggaatttt gataaacaaa aaactgcacg gcataatctt tcccggtttt
    13681 cattttccgt attcgcctat ccgtaccgca aatgctatac tgggaaaaat ttaccgattg
    13741 tgttttacgg catatttgcc gataggatgg aagagacaaa tgagcagaat ccggcaggct
    13801 tttgccgctt taaacggtga aaaggcattg attccttata ttacggttgg agacccggac
    13861 attggggcga ctttggcaat aatgcacagt atggtggaaa acggtgcgga tattttggag
    13921 ttgggcgtgc cgttttccga tccgatggcg gatggaccgg ttattcagcg tgcggcggag
    13981 cgggcgttgg caaacgggat ttcgctgcgc gatgtcttgg atgtcgtcag aaaattccgt
    14041 gaaaccgaca cgcaaacgcc ggttgttttg atgggatatt tgaaccctgt acataagatg
    14101 ggttatcggg agtttgctca ggaagccgca atggcgggtg tggacggcgt gttgacggtg
    14161 gattcccctg tcgaaaccat cgatccgctc tatcgcgagc tgaaggataa cggggtcgac
    14221 tgtattttcc tgattgcgcc gacgacgacg gaagaccgta ttaaaaccat tgccgagctg
    14281 gcaggcggat ttgtctatta tgtttcgctc aagggcgtaa cgggcgcggc aagtttggat
    14341 acggatgagg tttcgcgtaa aatagagtat ttgcatcagt atatcgatat tcccatcggt
    14401 gtcggtttcg gcatcagcaa tgcggaaagc gcgcgcaaaa tcggccgggt tgccgacgcg
    14461 gtcattgtcg gcagccggat tgtgaaagag attgaaaaca atgcgggcaa cgaggctgcg
    14521 gtcgtcggtg ctttggtcaa agagttgaag gatgccgtgc gctgattctg tcacgcattc
    14581 taaatatttt aggagttgtc catgagctgg ttagataaaa tcctgccacc caaaatcaaa
    14641 aaccgcagca aagacggttc ttccaatgtt cccgagggtt tgtggcacaa atgtccgtct
    14701 tgttcggcaa ccgtttattc gaccgagttg cagcagaaca atcaggtttg tccgaaatgc
    14761 aaccaccaca atccgttgtc ggcacgacaa cgcctgaacc tgcttttgga tgaggatggc
    14821 agggaggaag ttgccggaaa tgtcaaaccg atagatcctt tgaagtttaa agacagtaaa
    14881 aaatatcccg accgtttggg ggcggcacgc aagctgaccg gggaagatga cgcgctggtg
    14941 gtgatgaagg ggatgatgaa cggcctgccc gtcgtcgttg ctgcgtttga attccgcttt
    15001 atcggcggtt cgatgggttc ggttgtggga gagcgtttcg tacagggcgt ccgccgtgcg
    15061 gttgccgaca attgtccgtt cgtctgtgtg gcggcttccg gcggtgcgcg tatgcaggag
    15121 ggtgtgaact cgttgatgca gatgacgaaa accagtgccg cgctgcattt gctgacggaa
    15181 aaacgcctgc cgtttatatc ggtattgacc gatccgacta tgggcggcgt atccgccagc
    15241 ttcgcatttt tgggtgatgt tgtgcttgcc gaaccgaacg cgctgatcgg ttttgccggt
    15301 ccgcgcgtga ttgagcagac ggtgcgcgaa acgctgccgg aaggcttcca acgcgccgag
    15361 ttcctgctgg aaaaaggcgc aatcgaccag attgtcgacc gccgcgatat gaagcggcgc
    15421 atcagtgatt tgattacgct gttgtgccgt caggacaaag tttccgccgc ttgatggctg
    15481 atgaatcgaa taccgtctga aaccgatgtt tcagacggta tttttgtgtc tggttatttg
    15541 ttgtgcggct tcatcgacgg ggcatagcgt ccggcacgtt ctttcaggcg ttgtaccaaa
    15601 cctttcgtgt cggcgggtac accgccctcg cagaatgcct gatacaggac ggtgcgcagt
    15661 gcgtcgttgc ggcttaatgt accgcctatc ggtttccatt cggcgtttcg gggctgtatc
    15721 cagcggcggt tgaccgtgtc gccgtagccg aacactttat aggaggaagg tttgccgttg
    15781 ccgaacctga tggagaagcg ggcgcagaat atgccttcgg cagtcaggtt gtcgtagcct
    15841 ttgtcggagc ggatattgag aatgtagcgg atgctgccgt cgggcgcggg cataatttgc
    15901 aggctgtcga gcaggatttt cggctgtttg ccgtaatttt catccacata aatgtcgaac
    15961 cagccgtccg agtgcgtatc gggcagcggc ggcagtttgg cggtatgttc tttaaattcg
    16021 cgggcggcgg cctcttcggg cgtttcgcgg tagcgggtgt tgatcggcgt gtctttttgg
    16081 ctgaagccgg cagcgaggga cgtgccggcg gcgagtgcga gcagcaggag ggcggtgcgg
    16141 cgcataagtt tctccaaatt gaaaacggcg ttattttatg ggttggcaaa gggggctgca
    16201 agcaactggg gtataatctc cccccgattc ccatttttta aacggtacaa acgatgaaca
    16261 gcgaaacttt agacgtaacc ggattgaaat gtcccctgcc gattttgcgg gcgaaaaagg
    16321 ctttggcgca aatgcagcag ggcgacgtgt tgaccgttct ggcaaccgac ggcggcgcac
    16381 cgggggattt tgaggctttt tgccgccaaa ccggtcatgt gctgttggat gcttccgaac
    16441 aggacggcgt gttcacgctg gtcgtccaac acaaataaat gccgtctgaa atgcggatgt
    16501 cccgattgat gttattgctg tcggcatttg tgcttgctgc gtgtaatgcc caaaaccggc
    16561 aatccggtgt acggcatcag gatttcgcgc gtgtagccgt cgatttgttt cggagtgcct
    16621 gcgtcctgac tcaaggcagt tttgaatcgg tttccaagtt tgccgctgtc ggacattttg
    16681 tccctgtcgg aaaggaagac ctgtcccatc tgccgcccac tgttgcagaa cccgatgtac
    16741 aggcgttgtg gacgcttgaa cgcgatggcg gcatttatta tttgagcctg acgcgcgaca
    16801 gttgcagtgt gaaagccgag cgggcagaca gtgccgcgct gttggaacat tttgacggac
    16861 ttgtccgaca gccgccgaaa aatgccaata tagaattgag ggcggaacag tcggtgcagg
    16921 catcatttga aatccgccaa gtcagctatg cctggcggga agcgggcagc tccgaagaaa
    16981 ccgtattgac cgcacaaacc gccgattccc cggatttacc cgtacaggca gttttgaacc
    17041 tgacgcaccg ttcccacaac gggaaacccc taattctccc ctaacttcag acggcataaa
    17101 gtcggcatgc cgtctgaaca ttcaaataaa acaaaggaaa accatgcaaa ccctgaccat
    17161 tatccgcccc gacgatatgc acctgcacct gcgcgacggc gacgcgctca aagccgttgc
    17221 tccttatacc gcccgccaaa tggggcgtgc cgtcattatg cccaacctca aaccgcctgt
    17281 cgtcagcgtt gccgacgcgc ttgcctacaa agcgcgcatc atggcggcat tgcccgaagg
    17341 cagcgcgttt gagccgttga tgacgcttta cctgaccgac caagccacgc ctgcacttgt
    17401 gcgcgaagcc aaagccgccg gcatcgtcgc cttcaaactc taccctgccg gcgcgaccac
    17461 caattccgat tccggcgtaa ccgacctgtt caagctcatc cccgtgttgg aagaaatggc
    17521 gaaacagggc attttgttcc tcgttcacgg cgaagtaacc gaccccgaaa tcgacatctt
    17581 cgaccgcgaa gccgccttta tcgggcgcgt gatgaaaccc gttttggcgc aagtgccgaa
    17641 tcttaaagtc gtgttcgaac acatcaccac cgccgaagcc gcccgcctgg ttttggaagc
    17701 aggcgacaac gtagccgcca ccgttacccc gcaacacctc ctgctcaacc gcaacgacct
    17761 cttggtcggc ggcgtgcgcc cccatcattt ctgcctgccc gtactcaaac gcgaaaccca
    17821 ccgtcaggca ttggttgccg ccgttaccgg cgagaaggcg cacaaattct tcctcggcac
    17881 cgactccgcg ccgcacgcca agtccgccaa agaaaacgcc tgcggctgcg ccggtatgtt
    17941 cagcgcgatg accgccgtcg agctttacgc cgaagtgttt gaaaaagcag gcgcgttgga
    18001 caaactcgaa gcctttgcct cgaaaaatgg cgcaaggttt tacggcattc cggaaaacgc
    18061 cggcacgatc accctcgtca aacaaagcca aaccgtcccc gcaagtgttc cctacggcga
    18121 cggcgaactt gtcccgatgc gcgcgggcgg cgaaatcggc tggacggtgc agtattgatg
    18181 ggctggaaac aaaatgccgt ctgaagggag ggctttcaga cggcatttgt gttgctgact
    18241 gattgatacg tcaacggcgt ttgagtttgt tacgtttcgg ttatttccga taaattccca
    18301 caattttcaa atttcgccat tcccacgaag gcaggaatcc agaaattcga tgcgaccaaa
    18361 gtttatcaaa aacggcagca actcaaaaaa ccggattccc gcctgcgcgg gaatgacgag
    18421 attgaagttt cagaatttat ttgaaatacc caaaattcaa aaaaccaaat tcccacctgc
    18481 gtgggaatga cgaaacaaag aaagcagaaa taaggacata gaactttctt taaatttgtg
    18541 atgcatcaac ggcgtttggg ctcgtcgggg cggatttggg cggcgagttt gtcgaggatg
    18601 ccgttgacga atttgtgccc gtccgtgccg ccgaaggttt tggtaacttc gatggcttcg
    18661 ttgatgatga cggggtaggg tgtttcgggc atggcggaca gctcgtggca ggcggtcagc
    18721 aggacggcgc gttcgatggg attgaggtct ttttcgtcgc ggtcgagcag ggggcggatt
    18781 ttttggatgt agtccgctgc gttggtttgc gtaccgaaga aaagtttgtt gaacaattct
    18841 tcgtccgctt tggcaaagtc gggcatttcg cggatgtttt tagcaatttc gggcgcggcg
    18901 gtgcggttga taagggattg gtaaacggct tgtacggcaa gctcgcggga acggcggcgg
    18961 gctgttttca tgatttttcc ttgaaacggt tgggcggcac ggtatgccgt ctgaaacgga
    19021 aagggcgtat tggtgtacgc cctgtttgtt attcttcgtc ttcaaactgt tcttcgagca
    19081 gcaggttgac gaggttggcg cattcgacgg cgactttggc ggcatccgag gctttttctt
    19141 caatccgttc gattgcctgc gcgtcgtttt cggtggttag gacggcattg gcaatcggga
    19201 tgttgtagtc gagtgcgacg cggctaaccc ctgctccgga ttcgttggaa accagctcga
    19261 aatggtaggt ttcgccacgg atgacgacac cgatggcaat cagtgcgtca aacttttcgg
    19321 aagaggcaag gttcatcagt gcgatgggga tttcaagtgc gccgggtacg gtggcgacgg
    19381 taatgttttc gtctgccacg cccaattctt ggagggtgcg gcagcagact ttgagcattt
    19441 cgctgccgat ttcgttggtg aaacgtgcct gtacgatgcc gatgcggagg tgtttgccgt
    19501 cgaggttggg ggcgatggtg ttcattgggt gtcctttggt attcggaggt ttcggaatgc
    19561 cgtctgaagg tttcagtctt gcggctgcca gtcggcgacg gtttggaatg tgccgtcttc
    19621 ggcaagctcc catgcgctgc cttcgggttg ggaaagcagt gcggcggttt cagggttggt
    19681 tttggcgatg tcggcgaggc tgacgatgct gaagttgtcg ggatcgtcgg tgtattcgtc
    19741 ggtctcgtcg ccgctgaaga aacgccagcc gctgtcgttt tcaaaaacgg gggcttcgcg
    19801 gtagaggaag ccgacgggcc ggttttgttt ggcgacggtg ttggtggcga tacagcggtc
    19861 gagtgccgag gaaagtgctt gtgcaaatgc gttcattacg ggaatacgtt gggggaaaac
    19921 ttacggattt taccacgatt cgtgcgttgt cggcagacgg cggcggtttg gtggtacaat
    19981 gtgcgccgtt tgcagcctta aggtgtttct gtatttttgg agtatggaaa cgcattcggg
    20041 ctgttttttg cggaagacgg taatgaaaga cgatgttttg aaacagcagg cacacgcggc
    20101 gatacagaag agactgggct acgcgttccg cgatatttcg cttttgcggc aggctttgac
    20161 gcacaggagc catcatgcga agcacaacga gcggttcgag tttgtcggtg attcgatttt
    20221 gaattatacg gtggcgcgga tgctgtttga cacgtttccg aagttgaccg agggcgagtt
    20281 gtcgcggttg cgggcaagtc tggtcaatga gggcgtgctg gcggaaatgg cggcggaaat
    20341 gaatgtcggc gacggtctgt acttgggggc gggcgagttg aagagcggcg gcttcagacg
    20401 gccttcgata ctggcggacg cgatggaggc gatgtttgcg gcggtcagct tcgatgccga
    20461 tttcaacacg gcggaaaagg tggtgcgcca tttgtttgcc gaccgcgtcc ggtgtgccga
    20521 tttccaaaat caggcaaaag acggcaaaac tgctttgcag gaggcgttgc aggcacgccg
    20581 ctttgctttg ccgaaatacc gtatcgaaga gcaaatcggc catgccaacg acagtatgtt
    20641 tgtggtttcc tgcgatttgg gcgaactggg tttcgtgtgc cgtgccaaag ggacgagccg
    20701 caaggcggcg gaacaggagg cggcgaaaga ggctttgaaa tggctggaag agaagctgcc
    20761 gctgaagaag aaaaagaaat gaggcggcgc gtgaatatgc cgtctgaaat gtggatatga
    20821 aagcgaatat ggatattgaa accttccttg caggggaacg cgccgccggc ggataccgtt
    20881 gcggcttcgt ggcgattgtc ggtcgtccga acgtgggcaa atcaacgctg atgaaccatc
    20941 tcatcggtca gaaaatcagc atcaccagca aaaaggcgca gacgacgcgc aaccgcgtaa
    21001 cggggattta taccgacgat accgcgcagt tcgtgtttgt cgatacgccc ggctttcaaa
    21061 ccgaccaccg caacgcgctc aacgacaggc tgaatcaaaa cgttaccgag gcgcttggcg
    21121 gcgtggatgt ggtggttttc gtcgtggaag cgatgcgctt taccgatgcc gaccgtgtcg
    21181 tgttgaaaca actgcccaag cacacgccgg tcattttagt ggtcaacaaa atcgacaagg
    21241 acaaggcgaa agaccgttac gcgctggagg cgtttgttgc ccaagtgcgc gccgaatttg
    21301 aatttgcggc ggcggaggcg gtcagtgcga aacacggatt gcggattgcc aacctgttgg
    21361 agctgctcaa gccgtatctg cccgaaagcg tgccgatgta tcccgaagat atggttacgg
    21421 acaaatcggc gcgtttttta gcgatggaaa tcgtgcgtga aaaattgttc cgctatttgg
    21481 gcgaggaatt gccttatgcg atgaacgtcg aagtggagca gtttgaagag gaagacggtt
    21541 tgaaccgcat ctatatcgcc gttttggtcg ataaggaaag ccaaaaggcg attttaatcg
    21601 gcaaaggcgg ggagcgtttg aagaaaattt ccaccgaagc gcggttggat atggaaaaac
    21661 tgtttgatac caaagtattt ttgaaggtct gggtcaaagt caaatccggt tgggcggacg
    21721 acatccgctt cctgcgcgag ctgggtttgt agtttttctt gctgaacttt acgcaaatgc
    21781 cgtccgaaca ggtttcagac ggcattttgt ttcaatcggg aatatctttg ttaaaaacgg
    21841 gttgatatta tctgt
//
LOCUS       DAWHRS010000012        21268 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWHRS010000012 DAWHRS010000000
VERSION     DAWHRS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.012"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WIB82_000339"
     CDS             complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WIB82_000339"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ3841697.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFAAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDSSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WIB82_000340"
     CDS             complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WIB82_000340"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ3841698.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(3195..3896)
                     /locus_tag="WIB82_000341"
     CDS             complement(3195..3896)
                     /locus_tag="WIB82_000341"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ3841699.1"
                     /translation="MTLKTVAVIGAMEQEIELLRETMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPECVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            4082..5194
                     /locus_tag="WIB82_000342"
     CDS             4082..5194
                     /locus_tag="WIB82_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ3841700.1"
                     /translation="MTEKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            5228..6205
                     /locus_tag="WIB82_000343"
     CDS             5228..6205
                     /locus_tag="WIB82_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ3841701.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQYMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            6209..6559
                     /locus_tag="WIB82_000344"
     CDS             6209..6559
                     /locus_tag="WIB82_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ3841702.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            6564..7343
                     /locus_tag="WIB82_000345"
     CDS             6564..7343
                     /locus_tag="WIB82_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ3841703.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            7369..7584
                     /locus_tag="WIB82_000346"
     CDS             7369..7584
                     /locus_tag="WIB82_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ3841704.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREAKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQY"
     gene            7685..7996
                     /gene="grxD"
                     /locus_tag="WIB82_000347"
     CDS             7685..7996
                     /gene="grxD"
                     /locus_tag="WIB82_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ3841705.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            8102..8728
                     /gene="upp"
                     /locus_tag="WIB82_000348"
     CDS             8102..8728
                     /gene="upp"
                     /locus_tag="WIB82_000348"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ3841706.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLATELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            8751..9071
                     /locus_tag="WIB82_000349"
     CDS             8751..9071
                     /locus_tag="WIB82_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ3841707.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETWLLKP
                     LSAQ"
     gene            9322..9741
                     /locus_tag="WIB82_000350"
     CDS             9322..9741
                     /locus_tag="WIB82_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841708.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            9762..10523
                     /locus_tag="WIB82_000351"
     CDS             9762..10523
                     /locus_tag="WIB82_000351"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ3841709.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            10538..11872
                     /locus_tag="WIB82_000352"
     CDS             10538..11872
                     /locus_tag="WIB82_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ3841710.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NEKTASEPAAASDVKTIEAPSLPSERKPEQPAKKQTVPEKAGRSPSAKGERA"
     gene            11869..13092
                     /locus_tag="WIB82_000353"
     CDS             11869..13092
                     /locus_tag="WIB82_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ3841711.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDEIGEPQKAEAQRNLVLE
                     AVSDDERHAALEQHS"
     gene            13125..13565
                     /locus_tag="WIB82_000354"
     CDS             13125..13565
                     /locus_tag="WIB82_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841712.1"
                     /translation="MGSSDSFKEKKEIFEIGTPAYRQKLIDVWKKSINGNEKSWVLFE
                     NGTCVILLEPEKDLAKQAKEMLSKWGKVQIGTPSADFGIITLDSGDGYAVSCHHPEIF
                     TLILKEEGLDEDFKIGIEGRSHRDCDAEEPKVIHIEDKRTIETP"
     gene            13719..14783
                     /locus_tag="WIB82_000355"
     CDS             13719..14783
                     /locus_tag="WIB82_000355"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ3841713.1"
                     /translation="MISLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            14969..16348
                     /gene="radA"
                     /locus_tag="WIB82_000356"
     CDS             14969..16348
                     /gene="radA"
                     /locus_tag="WIB82_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ3841714.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(16623..17102)
                     /locus_tag="WIB82_000357"
     CDS             complement(16623..17102)
                     /locus_tag="WIB82_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ3841715.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(17234..17593)
                     /locus_tag="WIB82_000358"
     CDS             complement(17234..17593)
                     /locus_tag="WIB82_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ3841716.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(17632..21246)
                     /gene="recB"
                     /locus_tag="WIB82_000359"
     CDS             complement(17632..21246)
                     /gene="recB"
                     /locus_tag="WIB82_000359"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ3841717.1"
                     /translation="MSASIQAFDPLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDGALSDGIAAYCAE
                     HHKGDTFLPTLLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGYD
                     LNALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRQTYAQEKDGITMLKQNWRRVADNAPAGTDFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVAAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEMWLPAVAEM
                     AEACQKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPEGNICVIDYKSNHLGADASAYTRQAMDEAVAHH
                     HYYLQALIYAVASARYFKLRGQPPAAVSVRYLFLRGIDGKGGGVWHWDIDAAALEQIK
                     "
BASE COUNT         4887 a         5494 c         5959 g         4928 t
ORIGIN      
        1 gtaggaaaat ccgacaacgt tagatccgcc tgcaaaagtg aggaatagca aatgccgtct
       61 gaaactattt tcagacggca ttcttggctt cctggcctaa cggattgccg taccggccct
      121 gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg ccgacaacca gcttgtcatc
      181 cacaaatccc cagtagcgcg aatcggcact gttgtcgcgg ttgtcgccca tagcgaaata
      241 gcgtccttcg ggaactttgc acacgaaacc gctgctgtcg tcggcatatt ggcagtgttc
      301 caaaccgctt tgctctatgg aatatccgtt ttcagacata atatcggagg tatatttgcc
      361 caatacgggc agggaaacgg caggctgtcc ttcttttttc agaatattga aggatttgcc
      421 gtctagaccg ctgcggaaca tatccgtgtt gtggatttcg gaagggtcgg tgtcgtcggg
      481 ataacggtat gtgccgttag gaatatcgga agtgggtttg ccatttaccg tcaaaatctt
      541 atcccgatat tcgaccacat cgcccggaat gccgacaata cgcttgatgt aggtcatctc
      601 tggctgcaga ggataattaa aaacaacgac atcaccccgt tcgattttgc ctgtaggaat
      661 aaatatattg tttaaaacgg gtacgcgcag gccgtaggaa aatttgccga ccaaaatgaa
      721 atcgcccttg atcaggcccg ggcgcatcga gctggatgga atttggaacg gttcggcaat
      781 aaacgatcgg atgaggaaca ataccaaaac ggtagggaag aaactgccga aataatcgcc
      841 gaagtggctg ctttccgaga tttcgggatg agtcttcagg cggtatttat atacccccca
      901 agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg gcaaagctca taaacaggga
      961 caaagcggca aacacgccga ccgccgtcag gatataggcg tattccagac cggaactcca
     1021 ttcaccgttt tcctgccgct ttttgtcgct tttgaaataa aggatgatgc cggcaagcag
     1081 cgcggcagcc gcgcccgaca ttagcattgt gttcattgtt gttccttaat gcttaaaaac
     1141 ccgcctgtcc gtgcaaccgt tttaaggcgg caaattgcaa aatttgtttg cgggcgcgtg
     1201 cccctgaaat cagggcggtt tgaggggtgt tcccgacgcg ccgccctgtg tgccagagtt
     1261 atttgtcgct cacctgcaaa atcgccaaga acgcgctttg cggaatttcc acattgccca
     1321 cttgtttcat acggcgttta cctgcctttt gtttttcaag cagttttttc ttacgcgtaa
     1381 tatcgccgcc gtaacatttc gccaaaacgt ttttacgcag ggctttgacg ttttcgcggg
     1441 cgataatctg gctgccgatg gcggcttgga cggcgatgtc gaacatttgg cgcggaatca
     1501 gctcgcgcat ttttgatgcc agctcgcggc ctcggtgaac cgcgctttga cggtgcacaa
     1561 tcaggcttaa tgcatcgact ttttcgccgt tgaccataat atccagctta atcaaatcag
     1621 acggttggaa ctctttgaaa tggtagtcca acgaagcata accgcgcgaa gtggatttga
     1681 gtttgtcgaa gaagtccatg acgacttcgt tcatgggcaa atcgtaagtc agcatgactt
     1741 ggcggcccat gtactgcata ttgacctgaa cgccgcgctt ttggttacac aaagtcatga
     1801 cgttgccgac gtattcctgc ggcacaagga tggtcgcggt aataatcggc tcgagtatgg
     1861 tttcgatgct gccgatgtcg ggcagtttgg acgggttttc aacttcgatt ttttcgccgc
     1921 ttttcaacac gacttcgtaa accaccgtcg gcgcggtggt aattaaatcc atatcgaact
     1981 cgcgctccaa gcgttcctgc acgatttcca agtgcaacag acccaagaag ccgcaacgga
     2041 agccgaagcc caatgcctgg gaaacttcag gctcgaattt caacgaagcg tcgttaagct
     2101 gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg gctttccacg ggataaagtc
     2161 cggcgaatac ttggctttgt acttcttgga agccgggcag cggctcagtg gcagggttgg
     2221 caaccagtgt aaccgtatcg ccgactttcg cctgacccaa ttcttttacg ccggtaatca
     2281 aaaagcccac ttcgccggct ttgagttctt gtttttgaac cgatttcggt gtgaatacgc
     2341 ccaactgctc aacctgcgtt tccgccttgg tgctcataaa gcgcactttg tctttcagtt
     2401 tgatggtgcc gtttttcact cgaatcagca tgaccacgcc gacgtagttg tcaaaccacg
     2461 aatcgacaat caccgcttgc agcggcgcgt tttcgtcgcc ggcaggtgcg gggattttgg
     2521 caacgatttc ttccaaaacg tcttccacgc cgatgccgct tttggcggaa cactgcaccg
     2581 cgccgaccgc atcgatgccg atgatgtctt caatttcctg ctccacgcgt tcggggtcgg
     2641 cggccggcag gtcgattttg ttcaaaacag gcacaacttc cacgcccaaa tcaatcgcgg
     2701 tatagcagtt cgccacagtt tgagcttcta caccttggga cgcatcgacc accaaaagcg
     2761 cgccttcgca agctgacagc gaacgggata cttcgtaaga gaagtcgacg tgtcccggcg
     2821 tgtcaatcaa attaagctga tacacctgcc cgtcgcgcgc tttatagttg agcgcggcgg
     2881 tttgcgcttt gatggtaatg ccgcgctctt tttcgatgtc catggaatcg agtacctgcg
     2941 tactcatttc gcgcaaatcc aaaccgccgc aatattggat gaagcggtcg gcaagcgtcg
     3001 atttgccgtg gtcgatgtgg gcaatgatgg agaaatttcg gatatttttc attagagttg
     3061 ttttgaatgt cggacagtgg gtttgggaaa tgccgtctga acaaacggcg ttgcgtccga
     3121 atatcgggtg caacgtggaa atagcccgat attctaacgg aaaaccgctg ttttggcatg
     3181 atttgataaa ggttttataa agatttgacg atttctgcca ccatttttgc agaacttgcc
     3241 gccgccgttt tcaaaaattc gtcaaagctg atgtctgcct tttcgtccgc cgaatcggaa
     3301 accgcgcgga tgatgacgaa aggcgtttcc aactgatgac aggtttgggc aatcgccgcc
     3361 gcttccattt ccactgcttt gactttgggg aagtgcttgc ggatttccgc cacgccttcg
     3421 ctgctgtgga cgaagcggtc gccgctgaca atcaggcctt gttctaccgc cgcgccttca
     3481 aacacttccg ccgcctgttt tgctttgccg accaagaggc cgtctgaaac aaatacggcg
     3541 ggcagttgcg gcacttgtcc ccaaacatag ccgaatgcgg ttacatcgac atcgtggtgc
     3601 gcgatttctg tgccgaccac gacatcgccg actttcaaac ccttgcccaa accgcccgcg
     3661 ctgccggtgt taatgacgca ttccggtgcg aattgatgga taagccaagc cgttgcaacc
     3721 gccgcgttga ccttgccgat gccgctcaat gcaagcacca tgcgttttcc cgccaattcg
     3781 ccttcataag cggcaaactt gccgaaagag acggctttga cattttccat cgtctcgcgc
     3841 aaaagctcga tttcctgttc cattgcgccg ataacggcta ctgttttcaa agtcatattg
     3901 ctgacctgct gtgaatttcg gatagaatgc cgaattatac acgctaacac ggcaggattg
     3961 agtggaggcg gtttgtccat gccgtctgaa acggtttcag acggcatggc gggtttttgg
     4021 tagaatggga aggtacagat tgtttgaaga ttgagggacg aggatgttta ccgatgaaaa
     4081 tatgaccgaa aaggaagaac tgttcgcatg gctgcgccat atgaacaaaa acaaaggttc
     4141 cgacctgttt gtgacgaccc atttcccgcc cgcaatgaag ctggacggca aaatcacccg
     4201 catcacggac gaaccgctga cggcggaaaa atgtatggaa atcgcctttt cgattatgag
     4261 tgcgaagcag gcggaagaat tttcatcgac caacgagtgc aacttcgcca tcagcctgcc
     4321 ggacaccagc cgcttccgcg tcaatgcgat gatacagcgc ggcgcgacgg cgttggtatt
     4381 ccgtgcgatt accagcaaga ttcccaagtt tgaaagcctg aacctgccgc cggtcttgaa
     4441 ggatgtcgcg ctgaaaaaac gcgggctggt tatttttgtc ggcggcaccg gctcgggcaa
     4501 atcgacttcg cttgcctcgc ttatcgacta ccgcaatgaa aattcgttcg gacacatcat
     4561 caccatcgaa gacccgatcg agtttgtcca cgaacacaaa aactgcatca tcacccagcg
     4621 cgaggtcggc gtggatacgg aaaactggat ggcggcgttg aaaaacacgc tgcgtcaggc
     4681 gccggatgtc atccttatcg gcgaaatccg cgaccgcgaa acaatggact acgccattgc
     4741 ctttgccgaa acggggcatt tgtgtatggc gacgctgcac gccaacagca ccaatcaggc
     4801 actcgaccgc atcatcaact ttttccccga ggagcggcgc gaacaattgc tgacggattt
     4861 gtcgctcaac cttcaggcgt ttatttcgca acgcctcgtt ccgcgagacg gcggcaaggg
     4921 cagggtggcg gcagtcgagg tgctgctcaa ttcgcccctg atttcggagt tgattcacaa
     4981 cggcaacatc catgaaatca aagaagtgat gaaaaaatcc actaccctgg gtatgcagac
     5041 cttcgatcaa cacctttacc aattgtatga aaaaggcgat atttccctgc aagaagcatt
     5101 gaaaaatgcc gattccgcac acgatttgcg tttggcggta cagttgcgca gccgccgcgc
     5161 gcaaagttcc agccccgatt tggaactgct ctgatggcgg tatggatttc cggacggatg
     5221 gtttgaaatg atttatccgt ggcataatga gcaatggcgg cagattgcgg aacattggga
     5281 gcgtcgtccc aatgcatggc tgtttgccgg caaaaaagat acggggaaaa ctacatttgc
     5341 ccgctttgcg gcgaaggcac tgttgtgcga aacccctgca ccgggctgca aaccctgtgg
     5401 cgaatgtatg tcctgccatc tgtttggaca gggaagccat cccgattttt acgaaatcac
     5461 ccccttgtcg gacgaacccg aaaacggacg caaactgttg cagatcaaaa tcgatgccgt
     5521 cagggaaatc atcgataatg tgtacctgac ttcggtacgg ggcggtttgc gcgtgattct
     5581 gattcatcct gcggaaagta tgaatgtcca agccgccaac agtttgttga aagtgttgga
     5641 agaaccgccg ccacaagtgg tctttttgct ggtcagccac gcggcggaca aggttttacc
     5701 gaccattaaa agtcgctgcc ggaagatggt tttgcccgct ccttcccatg aagaggcatt
     5761 ggcatatctg cgtgaaaggg gtgtggcgga acctgaggaa cgtctggctt tccattccgg
     5821 agcgccgctg tttgatgagg cggacggtgt ccgtgcgttg cggattaaac tgttggatat
     5881 tttggcagaa ccaaggttgt tgaagatttt ggattacgcc gcgcttttcg ataaggagaa
     5941 acttccgctc gccgtatttg tcgggtggat gcagaaatgg ctggtcgatt tgggattgtg
     6001 cctgcaatac atgaaacccg tctattatcc cgcttatgaa gacaggctgc ttcagacggt
     6061 atccggtttc cgtccgcgca atgtatttgc ggcggaggat atgctcaaac agcttgcccc
     6121 ctacgggttt catactttaa atgtcaaaat gcagatcgag catctgctca tcaactattt
     6181 ggaattgaag aaagagaacg ggtgaattat gtcagacgga caaaatattc cggcaaaaat
     6241 gatgtcgttg cagctgaaag acatgaatct gctgtacagc tcctacatgc cgtttttgga
     6301 acacggcggt ctgtttgtgc agaccaacga cgtattttcc atcggggacg atattctgct
     6361 tgccgtagaa atcctcaact tccccaaact gttcctgccg accaaagtcg cctggatcaa
     6421 tcctgcgcgt acttcctcca aacccaaagg ggtggggctg gcattcacaa aacacgaaaa
     6481 ctgcctgaaa gtcaaagacc agatcgaagt cgaactgggc aacacaatcg gcggcagcag
     6541 acctacgttt accatgtaac gccatgcata tcatcgattc gcactgccac ctcaattttg
     6601 aaggtttgaa agaacgcctg cccgaagttt tgtccaacat ggaagcaaac ggcgtggggc
     6661 aggcgctcgc catcagcgtt agcagggaaa gttttgccga agtatccgct atcgccgaag
     6721 tttatgaaca tatttactgc acaataggcg tacatcccga cagcaaggaa gccgaagaat
     6781 tttctattgc ggaaatggtt gctgcagccg cccatccgaa agtggtcggc atcggcgaga
     6841 cgggtttgga ttattactgg tgcaaaggcg atttgtcctg gcaacacaaa cgctttgcag
     6901 accacatcga agcagccaat caaaccggac tgcccgttat cgtccatacg cgtgatgcgg
     6961 cggcggacac cttgtctatc ctgaaagaat gccgggttaa ttcgggcgtt atccactgtt
     7021 tttccgaaga catcggtttt gcccgtgcag caatggattt ggggctttat atttctttct
     7081 cgggaatcgt tacctttaaa aacgcaccct tggttcagga ggcggcgaaa tatgtgccgg
     7141 acgaccgcat tttggtggaa actgatgcgc cgttcctcgc ccccgttccc aaacgcggca
     7201 ggcagaacga accggctttt gtgcgccata ccgccgaaca tatcgccaaa ttgcggaacc
     7261 aaacattgga acaggttgcg gcatatacga cggaaaactt ttaccggctg tttaaaaaag
     7321 tacccgatat gcggaccgtc tgaccctgta ccgacgataa ggaaaaccat gaaggcaatt
     7381 catccgtatg catgtccgcg ctgctgccgg ctgcctgcca acacgtttcg gacaggcatg
     7441 gcaaattccg cttccaaatt ctgcattgcc aaaggcggca gacgggaagc aaaaaaagac
     7501 gaaagcggcg gcggatatgc cctgtgccat ttgccggaca gcaggattgt cgaggagtgg
     7561 gaatatttcc gttcacaata ttgatactgc gcgatatacg gcaaatattg tgggaagttt
     7621 ccgcttttgc gtataatgcg ccctacctga caaattttgt caactttatc aaaaggaata
     7681 agcgatggct tccatccacg accaaattaa agaagtagta acgacacacc gcgtcgtatt
     7741 gtttatgaaa ggtacgaagc agtttccgca atgcggtttc tcttcccgcg ccgtgcaaat
     7801 cctgaacgcg gcaggctgca ccgattacgt taccgtcaac gtattggaaa atcccgaagt
     7861 gcgccaaggc attaaggaat acagcgactg gccgaccatc ccccaacttt atgtgaacgg
     7921 cgagtttgtc ggcggttcgg acatcctgat ggaaatgtat gaggcaggcg agctgcaaga
     7981 gctgctgaaa gcctgatgga ttcggcaatg ccgtctgaac gtgtttcaga cggcattttc
     8041 ttttccggca aatcaaaaaa aagtataatg gcgcgtctca aaatcacatt ggaacaccgc
     8101 gatgaacgtt aatgttatca accatccgct cgtccgccac aaattaaccc tgatgaggga
     8161 ggcggattgc agcacctaca aattccggac gcttgccacc gagctggcgc gcctgatggc
     8221 atacgaggca agccgtgatt ttgaaatcga aaaatacctt atcgacggat ggtgcggtca
     8281 gattgaaggc gaccgcatca agggcaaaac attgaccgtc gttcccatac tgcgtgcagg
     8341 tttgggtatg cttgacggtg tgctcgacct gattccgact gccaaaatca gtgtagtcgg
     8401 actgcagcgc gacgaagaaa cgctgaagcc tatttcctat tttgagaaat ttgtggacag
     8461 tatggacgaa cgtccggctt tgattatcga tcctatgctg gcgacaggcg gttcgatggt
     8521 tgccaccatc gaccttttga aagccaaggg ctgcaaaaat atcaaggcac tggtgctggt
     8581 tgccgcgccc gagggtgtga aggcggtcaa cgacgcgcac cctgacgtta cgatttacac
     8641 cgccgcgctc gacagccact tgaacgagaa cggctacatc atccccggct tgggcgatgc
     8701 gggcgacaag attttcggca cgcgctaact gactgatttt cggagttgat atgaattttc
     8761 aagactatct cgccacattt ccttcaatcg accatctggg cggtttggac gttcaggatg
     8821 ccgacggcaa aacggttcac cacattcctg ccgttcaggg taagctcggt tcgctcaagc
     8881 tgtacaatgc tttggcggaa cgttttgacg gaaaattggg taaagaagcg gcagaacagg
     8941 gtttgatatg gtttgccgaa cacgttgccg acgcgcgtgc ccatccgggc aagcatccga
     9001 acatcgatct gctggaaaat gtcgtgcaaa gcggtgaaac ctggttgctc aagccgcttt
     9061 ccgcgcaata attttcgacc atgccgtctg aaatccgttt cagacggcat tttgtcggaa
     9121 agaagaccgt aaaacgggca ttttcttttc tatttcagga tacgggcaat gatgtttcaa
     9181 cacacaggac gacacataaa gcgccgccct atgtgttgcc ctaatttgga aggggttaca
     9241 cccttttcaa ataaaatctg atgctgctgc cacgaaggac ggatgtccga gtggcggggt
     9301 ttcaaccatt aaggaaatac gatgaaaaaa atgttccttt ctgccgtatt gcttctgtcg
     9361 gctgccgccc aaaccgtgtg ggcggatacg gtgttttcct gtaaaacgga caacaacaaa
     9421 tacatagaag tccaaaaaat caaccgcaat ctttacgaat attcgttcgg cagtgcggca
     9481 aaaaaagaaa ttgccatacg caacagcaaa gctgacctgt tggggcgttc cgacaggtgg
     9541 caaggtatgg gcagcggtcg ttgggcaacg atgaaattcc aaaacggcga atttatgtac
     9601 accatatgga caggcttcga ttccgtgact catacggaaa gcagcggtgt cgttgtggag
     9661 cgtaggggca aggaagtcgc acgggtcggc tgtacgccga aaaccgcgca ggcgaatttc
     9721 aacgatgacg atttttccta gtaatcgggg cggataaggc gatggaaaca gcgaaacccg
     9781 tcatgctgat tgtccgtccg tcgggcaggg ccgcagaaga tgtcgaaact tgcctgaatg
     9841 ccggttggcg cgcggaagta ttgagtccgg tcgaaatcga agcagatgct gccggactgg
     9901 aacttttgtc cgaacaatat gcccgtgcgg atgccgtgtt ttgggtcagt ccgaccgccg
     9961 ttgaaaccgc cgtcccgtac cttaaccttt cagacggcat aaaggcgcag attgccgtag
    10021 ggcagggcag ccgccgcgca ttggaacgct gtttggtcag aacggtcatc gcgcctgatg
    10081 acggcaacga cagcgaggcg gttttgcgcc tgccggtttg gaacagtctg cccgaaggtg
    10141 cgcgcgtatt gtttgtgcgc ggacacggcg ggcgggattt tttgatgaat gccttgcagg
    10201 agaaaggttt tcggacggag gtggcagaag tctatttcag acggcataaa cctttgaact
    10261 ttcaaaattt ccaaaccgaa aatattgccg ccgcctatat tacgtcgacc gagctggtgc
    10321 gcttgctgtt cgggcagctt ccgccgcaat tttcccgatt cttcaaatcc ttgctatact
    10381 ttacccatca tccgcgcatt gcggaggcat tgaagcgcga aggcgtgtgt tcggtcgaaa
    10441 ccgtccctac gctggaagcc gcgctttccc attcttccat ttccgtttca gacggcatgg
    10501 tctttcccgg aacctcaaat taataaggag caaaacggtg ggcgaacctg aaaacaaatc
    10561 atccgaaccc gtacgcgaga tacaggcatc aaaagaaatg ccgtctgaaa cctcttcccc
    10621 acgcaaagaa aacgaaacag aagtacacat tcctgccgct ccttttatcg tcaaacagtc
    10681 cggcagcaac gctttggcag tctgcgccct ggtattggcg gcattgggtt tgggtacaag
    10741 tggttttttg tttgtccaag gacagaatgt cttgaaaaac caagagctgg cattcaacca
    10801 aaaaatcgac aaagccgcct tgggcgagtc ggaaaacgcc gccctgttga aagacaacct
    10861 caaccggcaa gccgccatac aatcagagct cgaccgtttg gacggaaacg tcaaagcaaa
    10921 cggcgaacaa atcttggaaa tgcaaaaatc ctatcgcgag ttgaccaaag gacgcgccga
    10981 ttggctggtg gacgaaaccg agaccatact caatctggcg gcgcaacagc tggtgttgac
    11041 tggcaatatc caaacggcag tcggcgtatt ggagcatatc gacagccgcc tgtcccgttt
    11101 caatcaggca gagcttctgc cgatcaagca ggcggtcagc agcgacttgg cggaactgaa
    11161 aaaccgtccc tatgtcgata tttccggcac ggcattgcgc ctcgacaggc tggaaaccgc
    11221 cgtatccgga ctgccgctga tgctcgacgg cgtgctgaaa ccgggcgtac aggtgaagaa
    11281 cgaagccgct tccgcttcat ggtggcagaa cgtatgggaa aaatccctcg gcacattgaa
    11341 ggggctggtc gaaatccgac gtttggaaaa caacgatgcc atgctgattt ctcccgaaca
    11401 ggcatatttt gtgcgtgaaa acctgcgcct ccgccttttg gatgcgcgca ctgcattaat
    11461 gcagcgcaac agcgaagtct atcagggcga tttgaacaat gccgaagccg ccgtcagaca
    11521 gtatttcgat gccaagtctc ccgccacgca gtcgtggctg aaagaactgg cggaattgaa
    11581 ggcgttggat gtgcggatga ctgcggatga cggtttgaaa aacagcctaa atgccgtccg
    11641 cgcctatcgc gacggtacgc gcatgacggc ggcggaaaat caagaagcgg aacaggcggc
    11701 ttccgaaccg gcaaacgaaa aaacagcttc cgaaccggct gccgcatcgg atgtgaagac
    11761 catagaagca ccgtccctgc cttcggaacg caaaccggaa cagcctgcaa aaaaacagac
    11821 cgtaccggaa aaggcagggc gttcgccgtc cgctaaagga gaacgcgcat gaaaacggta
    11881 gtctggattg tcgtcctgtt tgccgccgcc gtcggactgg cgctggcttc gggcatttac
    11941 accggcgacg tgtatatcgt actcggacag accatgctca gaatcaacct gcacgccttt
    12001 gtgttaggtt cgctgattgc cgtcgtggtg tggtatttct tgtttaaatt cattatcggc
    12061 gtactcaata tccccgaaaa gatgcagcgt ttcggttcgg cgcgtaaagg ccgcaaggcc
    12121 gcgcttgcct tgaacaaggc gggtttggcg tattttgaag ggcgttttga aaaggcggaa
    12181 ctagaagcct cacgcgtgtt ggtcaacaaa gaggccggag acaaccggac tttggcattg
    12241 atgctgggcg cgcacgccgc cggacagatg gaaaacatcg agctgcgcga ccgttatctt
    12301 gcggaaatcg ccaaactgcc ggaaaaacag cagctttccc gttatctttt gttggcggaa
    12361 tcggcgttga accggcgcga ttacgaagcg gcggaagcca atcttcatgc ggcggcgaag
    12421 atgaatgcca accttacgcg cctcgtgcgt ctgcaacttc gttacgcttt cgacaggggc
    12481 gacgcgttgc aggttctggc aaaaaccgaa aaactttcca aggcgggcgc gttgggcaaa
    12541 tcggaaatgg aacggtatca aaattgggca taccgccgcc agctggcgga tgctgccgat
    12601 gccgccgctt tgaaaacctg cctgaagcgg attcccgaca gcctcaaaaa cggggaattg
    12661 agcgtatcgg ttgcggaaaa gtacgaacgt ttgggactgt atgccgatgc ggtcaaatgg
    12721 gtcaaacagc attatccgca caaccgccgc cccgagcttt tggaagcctt tgtcgaaagc
    12781 gtgcgctttt tgggcgagcg cgaacagcag aaagccatcg attttgccga tgcttggctg
    12841 aaagaacagc ccgataacgc gcttctgctg atgtatctcg gtcggctcgc ctacggccgc
    12901 aaactttggg gcaaggcaaa aggctacctt gaagcgagca ttgcattaaa gccgagtatt
    12961 tccgcgcgtt tggttctagc aaaggttttc gacgaaatcg gagaaccgca gaaggcggag
    13021 gcgcagcgca acttggtttt ggaagccgtc tccgatgacg aacgtcacgc agcgttagag
    13081 cagcatagct gattttggga aatatcttta tctgggagaa tttgatgggg tcttcagatt
    13141 cctttaagga aaagaaagaa atatttgaaa ttggaacgcc tgcttatcgc caaaagttaa
    13201 ttgatgtttg gaaaaagagc attaatggaa acgaaaaatc ttgggtgctc tttgaaaatg
    13261 ggacttgcgt cattttactt gaaccggaaa aagatttggc gaaacaagct aaagagatgt
    13321 taagcaaatg gggcaaggtt caaataggaa caccatctgc agattttggc attatcactt
    13381 tagatagtgg cgatggatat gccgtttcat gccatcatcc cgaaattttt acgctaatcc
    13441 taaaagaaga aggattggat gaagatttca aaatcggtat cgaagggcgc tctcatcgcg
    13501 attgtgatgc tgaagaaccc aaagttatcc atatcgaaga taaacgcacc attgaaaccc
    13561 catgaaaacc tgctgccgtt taatcatcta ctgatgatta cttaggcaaa tgtgcccgtc
    13621 cctccctttt cagacgacct ttcattgcgg aaaccgccgc aaaggttgtc tgaaaaccgt
    13681 tttccttccc cgttttacaa acaaaccgaa agccccacat gatctctttg aaaaacgaca
    13741 ctttcctccg cgccctgctc aaacaacctg tcgaatacac gccgatttgg atgatgcgcc
    13801 aggcggggcg ttatctgccc gaatacaaag ccacacgcgc gaaagcgggc agcttcctcg
    13861 atttgtgcaa aaacaccgaa ttggcgaccg aagttaccat ccaacctttg gaacgtttcg
    13921 atttggacgc ggcgattttg ttttccgaca tcctgaccgt ccctgacgca atgggcttgg
    13981 gactgtattt tgccgaaggc gaaggcccga aattcaaacg cgccctgcaa cacgaggccg
    14041 acatcgccaa gctgcacgtt cccgatatgg aaaaactgca atacgttttc gacgcggtaa
    14101 cttccatccg taaagcattg gacggccgcg taccgctcat cggcttctcc ggcagtccgt
    14161 tcacgctcgc ctgttatatg gtcgaaggcg gcggcagcaa agaattccgc accatcaaaa
    14221 ccatgatgta ctcgcgcccc gatttgctgc acaaaatcct cgataccaac gcccaagccg
    14281 ttaccgccta cctcaacgcc caaatcgacg cgggcgcgca ggcggtgcag attttcgaca
    14341 cttggggcgg cgtgttgagc gatgcggcgt ttaaagaatt cagcctcaaa tacatccgcc
    14401 agatcgtcgc cggactcaaa cgcgaaagcg aaggccgccg cgtgcctgtt atcgtatttg
    14461 ccaaaggcgg cgggctgtgg ctggaaagta tggcccaaat cggcgcagac gcattgggct
    14521 tggactggac gtgtaacatc ggcgaagcac gccgccgcgt cggcaagcaa gtcgccctgc
    14581 aaggcaactt cgacccgttc gccctcttcg gtacgccgga atccatccgc accgaggtcg
    14641 cacgtatcct agccgactac ggacacggca gcggccatgt cttcaacctc ggacacggca
    14701 tcaaccaaca cgccgacccc gaacacgcca aaatcttagt cgataccgta cacgagctgt
    14761 ctcggcagta tcacggcggg taagccggca ggaaaccgcc cgatatgccg tctgaagccg
    14821 agagatggcc ggttagggta aaaataaggc aatgcggcaa tatccgccgt gtacggatag
    14881 tacatgacgg cggcgttgtc gtattggcgc aatcccaacc gtccctatgt tcagacggca
    14941 tttttgtttt cagatgcagg gaaaaccgat ggcaaaaacg cttaaaaccc tttaccaatg
    15001 caccgaatgc ggcggcactt cgccgaaatg gcagggcaaa tgcccgcatt gcggcgagtg
    15061 gaacacgctt caggaaagcc ttgccgcgcc cgagccgaaa aacgcccgtt tccaatcttg
    15121 ggcggcggat acctcgaccg tccaatccct ctccgccgtt actgcgaccg aagtgccgcg
    15181 caatccgacc ggtatgggcg aactcgaccg cgtattgggc ggcggtttgg tcgacggtgc
    15241 ggtcatcctg ctcggcggcg atcccggcat cggcaaatcc acgctgctgt tgcaaaccat
    15301 cgccaaaatg gcgcaaagcc gcaaagtgct gtacgtttcc ggcgaggaat ccgcccaaca
    15361 ggtcgccctg cgtgcgcaac gtttggaact gcccacagaa ggtgtgaacc tgcttgccga
    15421 aatccgtatg gaggcgattc agacggcgtt gaaacagcat cagcccgaag ttgtcgtcat
    15481 cgactctatc caaaccatgt attccgacca aatcacgtcc gcccctggct ccgtgtcgca
    15541 ggtgcgcgag tgtgccgccc aactgacgcg catggcgaaa cagatgggca tcgccatgat
    15601 actggtcgga cacgtgacca aagacggcgc gattgccggc ccgcgcgtgc tggagcatat
    15661 ggttgatacc gtgctgtatt tcgagggcga ccaacattcc aactaccgca tgatacgcgc
    15721 catcaaaaac cgcttcggcg cggcaaacga actgggcgtg ttcgcgatga cggaaaacgg
    15781 tttgaaaggt gtgtccaacc cgtccgccat cttccttgcc agctaccgcg acgacacgcc
    15841 cggttcgtgc gttttggtta cgcaggaagg cagccgaccg cttttggttg aaattcaggc
    15901 attggtcgat gacgcgcacg gattcacccc caaacgactc accgtcggtc tcgaacaaaa
    15961 ccgcctcgcc atgttgctcg ccgtgttaaa ccgccacggc ggcatcgcct gcttcgatca
    16021 ggatgtgttt ttaaacgccg tcggcggtgt gaaaatcggc gaaccggcgg cggatttggc
    16081 ggtcatcctc gcgatgcttt ccagcttccg caaccgcccg ctgcccgaaa aaatggttgc
    16141 atttggagaa atcggtttga gcggtgaagt ccgccccgtc gcacgcgggc aagagcggct
    16201 caaagaagcg gaaaaactcg gcttcaaacg cgccatcgtt cccaaagcca atatgccgcg
    16261 caacgccaaa gagtttccga acctgaaaat ctacggcgtt tcgagtttgc aggaagccat
    16321 cgatatttgc cgtgacagca gggaataaac ggaaatgccg tctgaaatca ggtttcagac
    16381 ggcatttggt ttgtggcgga ttgaaacaag aaggcatacc ggcgacagat aagatttgcg
    16441 gcaaagttgc ctgtgatatg gcaaaacgca cacgcccgtc atccccgcaa gggcgggaat
    16501 ccggaatcgt ccgtttcggc aatgattgaa aatcacggta acccaaccgc ttggattccc
    16561 gacttcgtgg gaatgagggg cgtgtgtgtt tgatttccat ccgccatatg tcggcgacgg
    16621 gcttattcgc ctacggtttt ttgtatcagt ttttcggcgt ttgccaaagt gtttgccact
    16681 tcgtcaaaac cgatgcggct gccggcgatg agggcgcgcg tatcggtata ggcggcgcgt
    16741 actttgccgt ctgtttcggt aacgaggacg cgcaggggca gttgcagggc gaaggcgggg
    16801 tctttgacca tcagcggcgt gccggctttg ggcgtgccga agacgatgac ttttgccggc
    16861 tgcatcgtta agccgtttcg gtgggcggct tcctgatggt cgatgacggc aaaaatgtcc
    16921 atccctttgc tttttatggc ggtttcaagg cggctgacgg tttcgtcaaa actgtatttt
    16981 gaggtgaggg tatgcgtggt catagcggtt tcgttttggg tttgcggttc gctggcagga
    17041 tgtgccgaag cggttgaaat gcagagtgcg gatgcggcaa tcagggggag tatgtgtttc
    17101 atcgtatttc ctttttcctt ttcggtttgt ttatcaggct tcggacgggg cggcacgcgc
    17161 cgcgcccgat gttgcgccgc gtgccggggt gtcgtatgcc gtctgaaagc ctgtcctttc
    17221 agacggcatt gcgtcatttc atcccttttt tgagcaggtc ttcataaccg ccgtgattgg
    17281 caacattcgt atagcctgct tttttcagtt cttgaagggc ggcttcggca cgccgtccgc
    17341 tgcggcagta gaggttgacc ggcgtgtctt tgtcgggcgc ggcttcgtgt atgcggcgga
    17401 cgatttggtc gacggggatg ttgaccgcgt tgtgcaaatg accttcgcta aattcctgtt
    17461 cgctgcggac atcgatccaa acggctgaat gttgcgcggt ttgggcggcg gatacgggtt
    17521 tttgcggggc tgcctgcgcg gcaaaggcgg ctgaggcaat gagtgcggcg gtaatcaggt
    17581 gtttgatatt catagggttc tcctgcggtt gtcgtcttaa gggacgggaa gttattttat
    17641 ctgttccaaa gcggcggcat ctatgtccca atgccaaacg ccgccgcctt tgccgtctat
    17701 gccgcgtaaa aacaggtagc ggacggaaac ggcggcgggc ggttgtccgc gcagtttgaa
    17761 gtagcgcgcg gaggcaacgg cgtaaatcag tgcctgaagg taatagtgat ggtgcgcgac
    17821 ggcttcgtcc atcgcctgcc gcgtgtaggc ggatgcgtcc gcgccgagat ggtttgattt
    17881 gtagtcgatg acgcagatat ttccctcggg gtcttggcag accatatcga taaagccgtt
    17941 taaaaagccg ttgacggtgt ggaagtcgag cgtttcggcg gcggcacggc agacttcggg
    18001 cagcctgatg tcgtcgcggg caaaccagtc gcgcagccgt ttgaggccga agtcttcggt
    18061 gtggagggta aagcccattt cgggacagcg gcactcgggc gggatgtcgg acaggcagta
    18121 tgcccccgtc agcggcgttt tctggcaggc ttccgccatt tcggcaacgg cgggcagcca
    18181 catttcttca aacccgtatt tcttcagctt gtcggcaatg agagtttcct gtccggcggc
    18241 tgcttgtccg aatttgaaat cttcaagaat ttcgtgcagg cacagccccg cctgcgtgcc
    18301 tttcggaaaa tcgtgtatcg atatgccgtc tgaagccgtc ggcgtttcag acggcatcgc
    18361 cgctaccgag gtttcggcgg catccaagga cgggcaggca tcttcttcgc cgccgtcggg
    18421 cgtttgggta tggcggctta aggcggtaaa gctggtgtgg cggacaaatc ggaatctgcg
    18481 ttcgggaatg ctgtttgcgg caaattcggc ggtttgaccg gcgttgctgc gataggcggc
    18541 agggggcggc gcattttcgg tgaaggcgaa atccgtgccg gccggggcgt tgtccgccac
    18601 gcgccgccag ttttgtttga gcatcgttat gccgtccttt tcttgggcat aggtttggcg
    18661 gacggtttcg cggctgtctt gcggcaagcc ttcgattaga taggcgaggg ggttgtcggc
    18721 ggtatcggaa gaataggcgg cgtagatgtt gagctgttcc tcggcacgcg tcagcgcgac
    18781 ataaagcagg cgcaggcgtt ccgccatttc ttcatcggcg tattgtttct gttcgtcttc
    18841 ggacagttgc gcctttgcca acagttcggt tcggtttgcg ctttggtgga ggatttgcca
    18901 gtcggacggc ccggtatctt gcgcgtccca cgcaaacggg cagtacacca gcggatactg
    18961 taaacctttc gaggcgtgca tggtaacgat tttgaccaaa tcttcgtcgc tttccagacg
    19021 gatggcgcgg ttgtcgccgc cgttgttgcc ggccaggctg atttggtcgc gcagccattt
    19081 gtgcagcgcg gcggggttgc ggttttgcgc gtcttcggcg gcaagcagtt cgagcagttg
    19141 gaaatagttg gtcaggctgc gcccgttgtt ccggcttaag aggcgtgttt cgatgccgtg
    19201 tgtttgggaa aattgctgca tagcggcgaa aatgccgtat tgattccagt tgtcgagtgc
    19261 ggttcgggca gattccgccc aatgcaaaat ctcgctttcg ttttggttga agtcgtgcaa
    19321 ttgctgcgcg tcataaccga atatgccgct tgtcaggaca aagcgcagcg ttccggcgcg
    19381 gcgcggttcg agccagaagc cgatcagtgc ggaaagggcg gcggcttcgg gcgaggcgaa
    19441 cacagattcg cgcgaaagca ggacgctttg cacctgccgt tttttcaggg cagcggaaac
    19501 catcaccgcc tcgttgtgcg tgcgtaccag cacggcaata tcgcccgact gcaacgggca
    19561 gcctttgaaa ttcagacggc ctccggcggc ttcgttgagc gcgtgggcga tttcgtcggc
    19621 gcaatagtcg gcggcacggc ggcgcaaaac gtctttgttg gctttttcat tgtcgttttc
    19681 gtgcagccaa cgaacctgta cggcaggacg ttcgggggac aacctgcttt cggcacgcgc
    19741 cgcaccgact tccgaatagc cgatgttttc caaaacgaac gggcgttctt tgaggcggaa
    19801 cagcgcgcct atgctgccga taagcgcggc gtggctgcgg tagttggtgg cgagcgtgta
    19861 gcggtgccgc gcatcttccg ccgcctgaag gtaggcgtaa atgtccgcac cgcgaaagct
    19921 gtaaatcgcc tgtttgggat cgccgaccag aaacagcggg cggttttggg cgatgaagat
    19981 tttttggaaa atttcgtatt gcagcgggtc ggtgtcttgg aactcgtcga ttagcgcggt
    20041 ttcccagttt tcggcaacgg cgcgggcgag ggtttcggcg tgcggattgt cggtcagcgc
    20101 ggtgtggaca tcgagcagca ggtcgtcgaa accgcgttcg cggcgcgatt ttttcatctc
    20161 ggcaaggctg cggttgaggt atccgattaa atccagttgc agccggatta ttgttgcttc
    20221 ttccgcttct tcgagtgcgt tcaaatcgta cccgaagtct gccagtttct gcaattcggc
    20281 aaatactgcc gcatcgggcg ttttgccttt tttcagtccg gcttcgagtt tgtcggacgc
    20341 aagtttcaag agtctgtcgt gcgtgtcttt gtccagaaag ggcagttgtc cggcggcgga
    20401 tttttgtgcc agttctttaa aaaggttgcc gaagctgttt ttgcggtaac tgttgccgtt
    20461 gaggtcggga tggatgcgcc aaaagccggc ttccagctcg ggcagcaggc ggcagacggt
    20521 ttgccatgag gtttcggcgt tgcgctgcgc ctgtttcaaa tccgcctgcg gacggcggaa
    20581 attcaggtac gggcgggaca ggtaggcgcg gatttgggca aggacggttt gcggcacagc
    20641 tttgcgttta aacgccaatg cggcaagcac cggatcattg ctgacgcgtt cccgccaaaa
    20701 atcttgcgcc gggacaagca ggcggtcgcc gtcttcttcg gtcagttcga catcgaacgg
    20761 ggcttggcac aggaaggcgt agtcgcgcag gatgcgctga cagaagccgt ggatggtata
    20821 gatggcggcg ttgtcgaatt gcccgatggc ggccttgagg cggacaatca gacgcgtccg
    20881 gccctctttt tgcaaagcct gttttaagag tgtgggcagg aaggtgtcgc ctttgtggtg
    20941 ttcggcgcag taggcggcga tgccgtctga aagcgcgccg tctccaagtt cggcaatttc
    21001 tttgctttct aaaacctgca acacatcgtc caaacgcgcg cgcaggcgtg ttttcaactc
    21061 ggcggtggcg gctttggtga aggtaacgac caatacgcgc tcgacgtttt tttgttctaa
    21121 tacgatcagg cgcgtaaaca gggcggcgat gccgtaggtt ttgccggtgc cggcagaggc
    21181 ctcaatcagg ttggtgccgg caatggggac ggttagcggg tcgaatgctt ggatgcttgc
    21241 agacatagtg cgtgctcgga aaacggtt
//
LOCUS       DAWHRS010000013        19793 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWHRS010000013 DAWHRS010000000
VERSION     DAWHRS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.013"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(203..278)
                     /locus_tag="WIB82_000360"
     tRNA            complement(203..278)
                     /locus_tag="WIB82_000360"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="WIB82_000361"
     CDS             complement(371..730)
                     /locus_tag="WIB82_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ3841718.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="WIB82_000362"
     CDS             complement(753..1397)
                     /locus_tag="WIB82_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ3841719.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="WIB82_000363"
     CDS             complement(1399..1935)
                     /locus_tag="WIB82_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ3841720.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="WIB82_000364"
     CDS             complement(1947..3044)
                     /locus_tag="WIB82_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ3841721.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="WIB82_000365"
     CDS             complement(3047..4483)
                     /locus_tag="WIB82_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ3841722.1"
                     /translation="MENSLIIGLALAVLLVLLVMRAKQGKKTPKRKSQGVGNTQGQPP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEVEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIB82_000366"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIB82_000366"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ3841723.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="WIB82_000367"
     CDS             complement(5473..6120)
                     /locus_tag="WIB82_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ3841724.1"
                     /translation="MKTKFYNYQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTARCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIB82_000368"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIB82_000368"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ3841725.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFSRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            complement(7294..7431)
                     /locus_tag="WIB82_000369"
     CDS             complement(7294..7431)
                     /locus_tag="WIB82_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ3841726.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            7495..8570
                     /locus_tag="WIB82_000370"
                     /pseudo
     CDS             7495..8570
                     /locus_tag="WIB82_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
     gene            complement(8777..10243)
                     /locus_tag="WIB82_000371"
     CDS             complement(8777..10243)
                     /locus_tag="WIB82_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ3841727.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10441..11364)
                     /gene="speB"
                     /locus_tag="WIB82_000372"
     CDS             complement(10441..11364)
                     /gene="speB"
                     /locus_tag="WIB82_000372"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ3841728.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11463..13355)
                     /gene="speA"
                     /locus_tag="WIB82_000373"
     CDS             complement(11463..13355)
                     /gene="speA"
                     /locus_tag="WIB82_000373"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ3841729.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPAQHNQTASLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13522..13806)
                     /locus_tag="WIB82_000374"
     CDS             complement(13522..13806)
                     /locus_tag="WIB82_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ3841730.1"
                     /translation="MKKLLLAAVVSLSAATAFADDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13957..15765)
                     /gene="aspS"
                     /locus_tag="WIB82_000375"
     CDS             complement(13957..15765)
                     /gene="aspS"
                     /locus_tag="WIB82_000375"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ3841731.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(15823..16524)
                     /locus_tag="WIB82_000376"
     CDS             complement(15823..16524)
                     /locus_tag="WIB82_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ3841732.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16667..17815)
                     /locus_tag="WIB82_000377"
     CDS             complement(16667..17815)
                     /locus_tag="WIB82_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ3841733.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17978..18241)
                     /gene="rpsT"
                     /locus_tag="WIB82_000378"
     CDS             complement(17978..18241)
                     /gene="rpsT"
                     /locus_tag="WIB82_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ3841734.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18544..19686
                     /locus_tag="WIB82_000379"
     CDS             18544..19686
                     /locus_tag="WIB82_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3841735.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5091 a         5119 c         4593 g         4990 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gctgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaac aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caggtaagca gcttctcact gctgaccatt
     1261 tcgtccgcat ccgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgctg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttgatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ctcatggtaa cccaggctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgta ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcgactt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggcggctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcacca
     4441 gcaataccgc cagtgccaga ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg cgcactttgc ccgacggcat actgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt attaccttaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agtctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatgg gcgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg ccattagatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgccg tcaatttccc gaacggtgca acgcgctgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgaa tcaaatctgc
     5641 gcgcctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgtcaaa
     5701 cggacgggcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggcgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgttc gccttgataa ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcacg ccgcaaactg ccgcacttca tccattgtca tatctgccgt
     6181 caaagacaat cgtattctgg atgtaccttt tggtactgtc ggtggtctga tgggcaggca
     6241 ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc gaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacgggaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgca gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggtataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcgggtctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaagtat tgcgcaatgg gcaaaaacac
     8161 agggtaaaac catcgcgccc gagaaagtcg atgctgtact caaagaccgc accggatcgg
     8221 ccgtctgcct gacctgcggc aaaaacggca agatgaagat gccgctcaaa tcgctggcgt
     8281 ggatacaggg ttcttcctca tacagccaag ctgaagtcat tgaacaaact gaggaaacca
     8341 actctgccga aatatccgag acacgcacgc ccgaagtgcc tgatgtacac acgcccgagg
     8401 cacaaccgca tttaaatacc caatccgacg gcacgccgac tgcctatacc gaaccggctg
     8461 ccgattcatc gccgcaagta gaaacacctg atcaggctgc ttccgagccg gttgatgtat
     8521 tattttcaat agatgtgata cggcagggaa atttgcgttt aggtaattga ataaccttct
     8581 gattatttaa tgcttatgtt tatcagaagt tgataggcgg tttggttttt gtttggcttt
     8641 tcctacaccg ccgcctgccg ctttctgcaa cattcaagcg cacaaatatg ccatctgaag
     8701 gctttagacg gcatatttca cgataatagt cgcccaatca aataaacaca gattggaaat
     8761 ttattttatt tttcccttac caatacccca gaactttcca ccaaatgctg ccgataacga
     8821 aaaaaatcag aaaattgact acgctcatga taaaacccgc cttccaccat tctcccattg
     8881 tggtgtagcc cgaaccgaaa atcacaggcg aagtaccggt cgcataatga gtgagggtca
     8941 tcataatgtt ggatgcggcc gccatcatca gcgcggtcgg catcgccggg gcattcagtg
     9001 aaacggcagc agcgaaaaat gcgccgaaca tagcggtaat atgtgcagta gtactggcaa
     9061 acatataatg cgcatacata taagcaagca cgaggattac gcccgcagcc gtgccgctaa
     9121 cgcccaaacc gccgacactt tccgccaaca ctccggagaa ccatttaatc agtccgagtt
     9181 tatttaaaaa tgcggccatc ataatcaatg cgccaaacca aataatcgta tcccacgcgc
     9241 ttttttcttt caaaacatcg tcccaagtca atacaccgga aagcaaaagc aggcttaatc
     9301 cgataaatgc ggtggcggtg gcgttgatac taaaagcgtg attgccggta ataagggcgg
     9361 gaacatctgc ccacaacagc agcaagatac cgaaaatgac cgccataatg atttcgtctg
     9421 ccgacatttt acccatctcc ctcagacggt ctttggcaaa ttgaacggca ttgggcgttt
     9481 ctttaatttc aggcggatac aaaaaatata aaatcaaagg cataacgaaa aaggcgataa
     9541 cgccgggaac agccattgcc cacgcccacg ccccccaaga aagacggaaa ctactgccta
     9601 aattttcggc aatcaagttg acgattaaag ggttgggggc agttgcagta ataaacatag
     9661 ccgacgaaat gggattggaa tgatagttga ccaaagccaa atatttaccc atcttgcctt
     9721 ctgtgccttt tgcgggattg gagccgtaac tgccggcaat cgactgcata atcggatgta
     9781 taatgccgcc gccgcgcgcg gtattggaag gggtaacggg agccagcagc agttcggaaa
     9841 gagcgagact gtaaccgatg cccagcgttt ttcttccaaa aacggcgata aacaaatatc
     9901 cgatacgcat ccccagccct gttttgagca aaccgcgcga aatcataact gcgatggcaa
     9961 tcagccaaat caacggattg gcgaacgcac tcaacgcatc gctcatcgcc gcgcccggtt
    10021 tgtcggcggt tacgccggtt actgcgacca acccgacggc aataatcgac agcgcgccca
    10081 acggcatggc cttgccgata atggcggcaa tcacaccgac aaacatggcc agcagcgtcc
    10141 aagcctgagg cttgaccccg tcgggtacgg gcagtgccaa aaccagggcg cacaatactg
    10201 cggcaatggc gaggggtatc ggtttgaaac ccaatttcat catattgacc tccgtaaaaa
    10261 agaccgtccc gaaaaatcgg aaaaataata tttaactaat tgttttataa gatatattct
    10321 gatatttcac cgtctttccg atatgcggct ccgggcaact tttgtttcag tatttgaatt
    10381 ttcattagac tgaatacgcc gtttgaacgg cacggcgaaa acccggggga tgccggacgt
    10441 tcagtccttt ttcgcacctt gaaggtaaag catttccaag gcaattgtgg cgccggccaa
    10501 agcggtaatg tcggattggt cgtaagaggg ggcaacttct acaacatcca taccgacgat
    10561 gtcgagatcc gtcagcccac gtaggatttt taatgccctg tcgctgctca agccgccgca
    10621 tacgggcgta ccggtcccgg gggcgaacga cgggtcgagg cagtctatgt cgaaagtcag
    10681 gtaaacgggc atattgccga cggtttcttt gattttacgg acggtctctt caacactgtc
    10741 ttcattgact ttaggggcgg acaacacagt aaaaggcaat tttttactgt gttcggtgcg
    10801 tatgccgatt tgtacggaac gggacgggtc gatgaggcct tccttggggg cggtatagaa
    10861 catcgtaccg tggtcgtatt cgctgccgtt gtcgtaggtg tcggtgtgcg cgtcaaaatg
    10921 aatcagtgcg agtttgccga aatagcgggc gtgggcgcgc aacaacggta gggtaatgaa
    10981 atggtcgccg cccaaactca aacagcgttt gccggaagaa agtaatttgc cggcgtgcgc
    11041 ttccattttt tcgacaaaat ccctgctgtc gccaaaagaa aaaaccaagt cgccgcaatc
    11101 aataatgttc aggcgttcgc gcacatcaaa tgtccatgga aacctgcggt gctcccaagc
    11161 gaggttgacg gaggcgcgcc ggatggcttc aggaccgaaa cgcgcgccgg aacgccctga
    11221 aaccgccata tcataaggca cgccggtaat aacccaatcg gcatgacttt catacggcat
    11281 aaaattaagc ggcaggcgca aaaacccgaa attattggaa acgagggagt tgtcggtttg
    11341 tcctgccagt gtgctgtatt gcatcgtaat gattccttgt aattggtttc aatcggtcgt
    11401 gatgattggt gtttgagtaa gaaaatcggg cttcagacga catatccgat gccttgatgc
    11461 gtctattcgt cttccaaata ggtataacca ttaagccccg cttcgagttc ttttaagaaa
    11521 gacatagcct gcgaggcagg aaggtctgaa tgttcgattt gttcgcgata gcgtttcatc
    11581 agctctttcg gatcttgata aacgtattcg agcatatcgg caacggtgtt tccttcatcg
    11641 taatcgatga cggtaaattg tccgtcttcc cctacaacaa catcggcagt ggcagtgtcg
    11701 ccgaaaagat tgtgcatatt gccgagtatt tcctgatatg ctcccaccat aaaaaagcct
    11761 aaaagcggcg gctcttcttc gggataatca ggcataggca tcgtaccggc gatgccgtct
    11821 ccgtcgatgt agtggtcaat cgtaccgtct gaatcgcagg taatgtccaa caacacggcg
    11881 cggcgcgcaa tcggttcatt caaaccggta atgggacaaa cagggaaaag ttgatctatg
    11941 ccccaagcat cgggcaaaga ttggaagagt gagaaattga catacagctt atcggcaaaa
    12001 cgttcttgca attcgtcaat aatggttcgg tgagaccggt gtttttcatt aaacaattcg
    12061 ccgacttcat gacagatatt taaatacagt tgctccgccc acgcacgttg cgccaaactc
    12121 aacagcccga cattatactg attatgcaca tcagcaagat caaactgccc ttcgtgtatc
    12181 cagctgcgta aggaacgttt ttcccgcgag gcggaaatat ccgtccaagt ttcccacata
    12241 ctgtgcaaca cacgcggtgc ttcgggcgat ggcgcatcca gccggcgcgg tttgtaacgt
    12301 tcaacgccta taacattagc aaccaaaacg gcgtgatgtg cggtaatgcc gcgcccgctc
    12361 tcggtgatga ttgtcggatg cggcagcccg tgttcgagac aagcctgact gatgccccat
    12421 acgactgtgg cggcatattc gttgaggctg taattaacgg aacaatccga ttgtgtgcgg
    12481 tttccttcgt aatccacgcc aagcccgccg cctacatcaa aacagcggat atttaccccc
    12541 agtttgtgca actcaacata aaaccgagcc gattcgtgta cacctgtggc aacatcacgg
    12601 atgttcccaa gctgcgagcc caaatggaaa tgcaaaagct gcaggcaatc cagcctgttt
    12661 ttttgtttca aaatatcgac cagttgcaaa acttgggaag ccgacaagcc gaattttgat
    12721 ttttccccac ccgaagactg ccattttccc gaaccttggg aagccagtct ggcgcgcaca
    12781 cccaaacggg gcttgatgcc gagtttttcc gcctcttcca ataccatttg tatttcggac
    12841 agcttctcaa tcaccaaata aacctgatgc cccagttttt cgcccatcaa ggcgaaacgg
    12901 atatattcac ggtctttata gccgttgcag acgattaatg tttgccgggt gccggcgtgt
    12961 gccaaaaccg ccatcagttc ggctttagaa ccagcttcca aaccatgcgg ttgtccgctt
    13021 gacataagcg attcgatgac gcggcggtgt tggttgacct tgatagggta aaccaaacaa
    13081 taaccgccct tatagccgca ctcttcccgt gccgtctgaa aggcgcggtt aatgtcgcgg
    13141 aggcggtgtt cgaggatttg cggaaaacaa aacaaaacag gcaggcgcgc ctgatgtttt
    13201 tgttgcacgg cttcagtcag tttttgcagt gaagcagttt gattgtgttg cgccggattg
    13261 gggcggacga tgatttcgcc ggaatcgtca acatcataat aacctatgcc ccaatgatta
    13321 atgttgcaca cttcacggat ggtaaggata ggcataataa acctgctccg tctgtcgtgt
    13381 tgaaaaggaa tgattataac aaatcagcgt gaaatgtcat ttttttaata agaaaagcct
    13441 gcctcatacc tgatgaggaa caggcaaaat gccgtctgaa cgcttcagac ggcattttgg
    13501 ttcatctttc catcaaagga gtcagcgatc gagctgctct ttgatgattt tcaggtcggg
    13561 gtaagacaac acgatgtcgt attcgcggcc gtctttatag gcttccactt ccaaaacagg
    13621 tttgccccaa tggtcgtcgg catcgacatc gtgaacctga taaccgcgct gttccaacat
    13681 ttttacggct tttgtgcggt tttgttcaaa atggggatca ccgtaaatct gacgctcggc
    13741 agaatcgtcg gcaaatgcgg ttgcggcact cagggaaaca acggcagcca ataacagttt
    13801 tttcattttc agtcctttct taatcggttg attgaataag atgtgttttt caatgctgcc
    13861 attaaaacac agcaaaatta ggtttgaatt agcaggaagt taagaagcgt aaatgccgtc
    13921 tgaaaaaaac agagccgtca aacggctccg ttttccttat gcttctttgg tttcagccac
    13981 cttttgacgc aggcgcaagc tcaattcgcg caactgcttg tcgtccacgc tgttgggcgc
    14041 gttggtcagc aggcattggg cacgttgtgt tttcgggaag gcaatcacgt cgcggataga
    14101 ttcggcgccg gtcatcaggg taaccaaacg gtcgaggccg aatgcaaggc cgccgtgcgg
    14161 aggtgcgccg aatttcaggt tgtccaagag gaagccgaat ttctcttgtt gctcttcagg
    14221 gctgattttc agcgcggcaa acactttctc ttgtacgtct gcacggtgga tacggataga
    14281 gccgccgccg atttcccagc cgttcaatac catgtcgtag gcgcgtgcca aacaatttgc
    14341 cgggtcggaa accatcaggt cttcatgacc ttcttttggc gcagtaaacg gatggtgtac
    14401 ggcaacgtag cggtcggctt cttcgtcgta ttcaaacatc gggaagtcga caacccacaa
    14461 aggtttccat tcgtctgtga agtagccgtt ttccgcgccg tgttctaaac cgactttaat
    14521 acgcaatgcg ccgatggctt cgttcacgac tttggttttg tctgcaccga agaagataat
    14581 atcgccgttt tgtgcgccgg tacgctcgat gatttctttc agggcattct cagacaggaa
    14641 ttttacgatt ggagattgca ggccgctgtc ttcgccgttg gaaaggttgc ccacatcgtt
    14701 tactttgata tatgccagac ctttcgcacc gtagatgccg acaaatttag tgtattcgtc
    14761 gatttctttg cggctgaatt tcgcaccgtt cggcacacgc agggcaacca cgcggccgcc
    14821 tttcatgtcg gctgcgccac ggaagacttt gaattcttcc gttttcatca ggtcggtcaa
    14881 ctcggtgaat ttcaagttga tacgcatgtc cggtttgtca gagccgtagt agaacatggc
    14941 ttcagagtaa ggcatgcgtg ggaagtcgcc caagtccacg cccagcgcgt ctttgaagac
    15001 ttgtttcgcc ataccttcgg tgatgtccat gatttcatcc tcgtttaaga acgaggtttc
    15061 caagtcgatt tgcgtgaatt caggctgacg gtcggcacgc aagtcttcgt cgcggaagca
    15121 cttggtgatt tggtagtaac ggtcgaaacc cgccaccatc aacagttgtt tgaataattg
    15181 cggcgattgc ggtagcgcga aaaactcgcc cggatgaacg cggctcggca cgaggtagtc
    15241 gcgcgcgcct tcaggcgtgg agcgggtcag catcggggtt tcaatgtcga tgaaaccttg
    15301 cgcgtctaga taacggcgaa cgcccatagc gacttggtaa cgcaggcgca ggttgcgctg
    15361 catcacaggg cggcgcaggt cgataacgcg gttggtcagg cgaacgtttt cgctgatgtt
    15421 ttcatcgtcg atttggaacg gaggcgtagc ggcagcgttc aacacttcga tttctttggc
    15481 aagaatctcg attttaccgg aaatcatttt gtcgttggtc gtgccttcgg gacggttgcg
    15541 tacgcggccg gtaatgctca aaacgtattc gttgcgggag gaatcggcag cggcaaacgc
    15601 ttcgggcgtg tcgggatcga tcacgacttg gacgatgcct tcgcggtcgc gcaggtcgat
    15661 aaaaatcaca ccgccatggt cgcgtcgacg gtgtacccag cctttgacgg taacggtttg
    15721 gtctaagtat tgctcgctaa tcaggccgca atagttggta cgcataaaat caccttttat
    15781 tgatttaaac tgaaaacaga aaatgccgtc tgaacggcgg ctttattgtt gttcgggcaa
    15841 atccgccttt tcagacggca taggtcctgc caatgttttg acgggcaggt cgtcagggat
    15901 gaccataccc agcgaaatca catatttcaa cgcttcgtcc acgctcatat cgagttcgcg
    15961 cacatcactt ttctttacca taatatagta accgccggtc ggattcggcg tggtcggaac
    16021 atacacggaa agataatcgc cgtccttcgg caatgcggcc ttaaccgcat tcgacacctg
    16081 accggacacg aatgcgattg tccaaatacc cgattgggga aacggcacga gtactggtgt
    16141 tttaaacgaa cggctgctgt cggacagcaa cgattcggat acttttttca cactcgaata
    16201 gatggacttc acaaccggaa tccgccccaa caggctgtcc cacgcggcaa gaatctgccg
    16261 gcccaatacg tttgcggcaa acaatccggt tacaaacaat acggcaatgg caacaataac
    16321 gcccagcccc gggatattaa accccaaaac atattgcggc cgccattgct tcggcagcag
    16381 gttgacaagc tggtcggacg cggaaacgat ataggaaacc acccaaaccg ttaccgcaat
    16441 cggcagccag accaaaatgc ccgtaatcag atattttttt aacgccttgg cagctttgcc
    16501 gccttcggcc gcaggttccg tcatcttgct tgattccgac aaagtccgta caaaccgcac
    16561 attatacgcg tttgcccgga ttcaaacgaa atttttatcc cgccccgcca aaccgccggc
    16621 ggcttcagac ggcacggcaa cttgatatat cgtctgaaca cgcggttcag atgccgtcca
    16681 agtcgttgaa catcaacccg ataccgatac cgttctgctt gtggttgtag tcgatcaggc
    16741 tctcgccgta accgtggaat ccgcgtacca cgcctttgag tttgccctta atcggaaacg
    16801 tgtaggcggc ttcaatcgcg ccgtagcccg ttttggggtt gtagcgcaat acggaataca
    16861 cattctgcct gtcgttcagg cggtactgca gcttcacgtc gccatacccc atatagtcgg
    16921 caatatcggg attgtcgttt ttatcgccgc tctgatcgaa cgcacgcacc cacacgcgcg
    16981 gaatcaccgt caatttgccc cattccatgc ctgccatggc gtaaatcctg ttccacgaac
    17041 gcgattcggg acggctctgt ccgttggact ggtggacaaa acccgcaccg agcatacgca
    17101 gcctgccgcc gaacggcaaa tccgccttca caggctgggt caggaaaatt tcaggtttgt
    17161 aatccgtatt gcggaacggc gcggatttcc tgccttggtt gtaaatctgc caatcggatc
    17221 tttgggtgta gccgaaccac agatccgcgc gggttttaaa caaatcttcg gcaattttgc
    17281 ttttgaacga aacctgcaat ttggtttccg cacgtttctg ctgtccgaat ttttcctgta
    17341 cagtcgtacc gcgcgtcggc gaacccgggg catagttggg cgaattgttg taccagagcg
    17401 gcataaggta catcggattg tgttcgcgta cgcccaacag cccgcgcaaa tcgtttttgt
    17461 ccaagtcgta catcaggctc aaaggcgtat agatgtcggc ggtttcgccc gcactgtcgg
    17521 caggaagcgc atccccgcct ttttcaacaa caatgaccgc ctcgccctta tccaggctgc
    17581 tgcggacggt ttccgtcaga ttgagtacgg ctttcgactc ctgcccttcc tgccctgccg
    17641 aagacggaag ctgtgccgca aaaatcctgt cgtaacacgc caaacgcgta acattgtccg
    17701 tcaaagcggc gcattgcagc gcggtctctc caaaagcgga tgccatcggc aacagtcctg
    17761 tcaaaagaat atagcgcata ttccgtgtat tcatctccgc ccccattgtc ggcatattgg
    17821 ttttcaaacg gcattttata gcggattcgg ataaaaaccg caccctttcc gccatttcgg
    17881 gattttgccc ccgcaataca gaaaaccccg aaaccgtcag gcttcagggt tttccgctta
    17941 tcgcgtatca accgccttct tggcggtttt gcaaaaatca agccagtgct tttacttttg
    18001 cagacagacg gcttttgtgg cgagccgctt tgtttttgtg gaacacgccc ttgtcggcga
    18061 tgcggtcgat gactttgaca gactcttggt aaaccgcttg tgcggcagct ttatcgcctg
    18121 cttcgactgc tttcaatact tttttcactg cggtgcggaa tgcggtacgc aggctagcgt
    18181 tgtgggcgcg ttgtttgacg gactggcggg cacgtttgcg tgcttgtgcg ctgtttgcca
    18241 tattggaatc tcctggaaga aaaataaatt ctgtaaacgc gcaattttaa agggggttta
    18301 cattatatgc aagccttttc tctctatccg cccgtcggac accctgacag cgcaaaaaaa
    18361 cggcaaaatc gtgcggacgt tttccccctc ttttgaaaac agggtaattt tttagggaaa
    18421 tcagtccaat atcactttga gtttgcacaa atttacttta caatgcccaa accttgcgcc
    18481 ggggtgttta tggggcgcgg ggttttcttt ttttccaacc tctttcatca ggaatctgcc
    18541 cgtatgaaaa aatccgtatt agccgttttg gccgcgctgt ctctggcagc ctgcggcggc
    18601 agcgaaaaaa acgccgtgca acctcaagca gacgctgcgt ctgctgccaa tgccgaagcc
    18661 gctgccaccg atactctgaa catctacaac tggtcgaact atgttgacga gagtacggtc
    18721 gaagacttta aaaaagccaa caatctgaag ctgacttacg acttgtacga aaacaatgaa
    18781 acgctggaag ccaaaatgct gaccggcaaa tccggttacg atttggttgt gccgggcatc
    18841 gccttcctgc cgcgccaaat cgaggcgggc gcgtatcaaa aagtcaacaa agacctgatt
    18901 cccaactata aaaacatcga tcccgaactc ttgaaaatgc tggaaaccgc cgacccgggc
    18961 aaccagtatg ccgtccccta tttctccggc gtgaacacga ttgcgattac ggcgaagggc
    19021 aaagagcttt tgggcggcaa gctgcccgaa aacggctggg atttgctgtt caaacccgaa
    19081 tacacccaca agctgaaatc ctgcggcatc gccctgtggg acaccccgag cgaaatgttc
    19141 ccgattttgc tgaactactt gggcaaagac cccaaaggtt cgaatcctga agatttgaag
    19201 gcggcggcgg aagtattgaa gtctatccgt ccggatgtca aacgtttcag cccgtccatc
    19261 atcgacgagc tggcgcgcgg cgacatctgc ctggcggcag gcaacggcgg cgatttgaac
    19321 ttggcgaaag cacgttccga agaagtgaaa aacaacgtcg gcatcgaagt gctgaatccg
    19381 aaaggcatgg gcttctggat tgagtcttgg ctgattcccg ccgatgcgaa aaacgtcgcc
    19441 aatgcccaca aatacatcaa ctacacgctc gaccccgaaa tcgcggcgaa aaacggcatc
    19501 gccgtaacct ttgcccccgc cagcaaaccg gcgcgcgaaa aaatgcctgc cgagctggta
    19561 aacacccgtt ccatcttccc gaacgagcag gatatgaaag acggtttcgt gatgccgcaa
    19621 atgagcacgg atgcgaaaaa actgtctgtc agcctgtggc agaaaatcaa agtcggcacc
    19681 aactgatttg aagcattaaa aatgccgtcc gaacatcgtt cggacggcat tttatattgg
    19741 attgaaatag aaatatttat atagtggatt aacaaaaatc aggacaaggc gac
//
LOCUS       DAWHRS010000014        19757 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWHRS010000014 DAWHRS010000000
VERSION     DAWHRS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19757
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.014"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..170
                     /locus_tag="WIB82_000380"
     CDS             <1..170
                     /locus_tag="WIB82_000380"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ3841736.1"
                     /translation="VVKATDLRASASLVIAGLAARGETVVEQIYHLDRGYENIEKKLG
                     SVGAKIERVSG"
     gene            251..1429
                     /locus_tag="WIB82_000381"
     CDS             251..1429
                     /locus_tag="WIB82_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ3841737.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMIHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDDMILDIGPKSAAELAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(1496..1744)
                     /locus_tag="WIB82_000382"
     CDS             complement(1496..1744)
                     /locus_tag="WIB82_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ3841738.1"
                     /translation="MLTPEQVKAMIEDVAKCEHIKVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(1834..2751)
                     /gene="ftsX"
                     /locus_tag="WIB82_000383"
     CDS             complement(1834..2751)
                     /gene="ftsX"
                     /locus_tag="WIB82_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ3841739.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPEEIV
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(2748..3398)
                     /gene="ftsE"
                     /locus_tag="WIB82_000384"
     CDS             complement(2748..3398)
                     /gene="ftsE"
                     /locus_tag="WIB82_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ3841740.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(3395..3586)
                     /locus_tag="WIB82_000385"
     CDS             complement(3395..3586)
                     /locus_tag="WIB82_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841741.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            complement(3594..4076)
                     /locus_tag="WIB82_000386"
     CDS             complement(3594..4076)
                     /locus_tag="WIB82_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ3841742.1"
                     /translation="MKYLNLAAITLGATFAAHTASADELAGWKDGAAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            4200..4553
                     /gene="arsC"
                     /locus_tag="WIB82_000387"
     CDS             4200..4553
                     /gene="arsC"
                     /locus_tag="WIB82_000387"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ3841743.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNNALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            4550..5206
                     /locus_tag="WIB82_000388"
     CDS             4550..5206
                     /locus_tag="WIB82_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ3841744.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFTSSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            5322..6716
                     /locus_tag="WIB82_000389"
     CDS             5322..6716
                     /locus_tag="WIB82_000389"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ3841745.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRRFDRYKEVIAELLEKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITANGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            6737..7132
                     /locus_tag="WIB82_000390"
     CDS             6737..7132
                     /locus_tag="WIB82_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841746.1"
                     /translation="MPSDVGIRLQTAFKWKLKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNKLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            7136..7627
                     /locus_tag="WIB82_000391"
     CDS             7136..7627
                     /locus_tag="WIB82_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3841747.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDI
                     DKV"
     gene            complement(7751..10345)
                     /gene="mutS"
                     /locus_tag="WIB82_000392"
     CDS             complement(7751..10345)
                     /gene="mutS"
                     /locus_tag="WIB82_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ3841748.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPETHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELEDQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            10789..11793
                     /gene="gap"
                     /locus_tag="WIB82_000393"
     CDS             10789..11793
                     /gene="gap"
                     /locus_tag="WIB82_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ3841749.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSILERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(11863..11982)
                     /locus_tag="WIB82_000394"
                     /pseudo
     CDS             complement(11863..11982)
                     /locus_tag="WIB82_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12051..12731)
                     /gene="tsaA"
                     /locus_tag="WIB82_000395"
     CDS             complement(12051..12731)
                     /gene="tsaA"
                     /locus_tag="WIB82_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ3841750.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAKACIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLNEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            12845..13480
                     /locus_tag="WIB82_000396"
     CDS             12845..13480
                     /locus_tag="WIB82_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ3841751.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            13517..14485
                     /gene="waaC"
                     /locus_tag="WIB82_000397"
     CDS             13517..14485
                     /gene="waaC"
                     /locus_tag="WIB82_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ3841752.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            14712..15461
                     /locus_tag="WIB82_000398"
     CDS             14712..15461
                     /locus_tag="WIB82_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ3841753.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            15472..16407
                     /locus_tag="WIB82_000399"
     CDS             15472..16407
                     /locus_tag="WIB82_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ3841754.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGNGASAVV
                     EFAKQVAGVKKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQVDSEKLVLTFRATAFDAAPVQGGH
                     AEAVNVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            16470..17081
                     /locus_tag="WIB82_000400"
     CDS             16470..17081
                     /locus_tag="WIB82_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ3841755.1"
                     /translation="MITTYKTITSLTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(17133..17450)
                     /locus_tag="WIB82_000401"
     CDS             complement(17133..17450)
                     /locus_tag="WIB82_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ3841756.1"
                     /translation="MQKRIEEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            17608..18879
                     /gene="purD"
                     /locus_tag="WIB82_000402"
     CDS             17608..18879
                     /gene="purD"
                     /locus_tag="WIB82_000402"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ3841757.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            18913..19515
                     /locus_tag="WIB82_000403"
     CDS             18913..19515
                     /locus_tag="WIB82_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ3841758.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         4800 a         4459 c         5522 g         4976 t
ORIGIN      
        1 cggtcgttaa ggcgacggat ttgcgtgcgt ccgccagcct cgttatcgcc ggtttggcgg
       61 cgcgaggcga aaccgtggtc gaacagattt accacttgga tcgcggttat gaaaatattg
      121 aaaaaaaact cggcagcgtc ggcgcgaaaa tcgagcgcgt atccggctga tcgggttgcg
      181 atgccgtctg aagccttcag acggcatcaa ttgtttctgt atcgcaaaca ctgattgaaa
      241 aggactgatt atggcatttt taaaactgac cgaacaaaac gtgcagggca aaaccgtcct
      301 catccgcgcc gatatgaacg tgccgttcaa agacggcaaa atcagcgacg acacccgtat
      361 ccgcgcctcg ctcgcgtcca tccaatactg catggacaac ggcgcatccg ttatcgtgat
      421 gatccacttg ggtcgcccga ctgaaggcga gttccacccc gaagacgacg ttgcgcccgt
      481 tgccgcgcat ttgggcggtc tgttgggcaa agacgtgaaa gtattgaacg actggcgcga
      541 aaacaaaccg gctttgaacg cgggcgatgt cgtgatgctg caaaacgtgc gtatcaacaa
      601 aggcgagaag aaaaacgatt tggaactggg taaagcctac gcttctttgt gcgacgtgtt
      661 cgtcaatgac gcgttcggca ccgcgcaccg cgcccaagct tcgaccgaag ccgtcgctca
      721 agccgcgccc gttgcctgcg ccggcgtatt gatggcgggc gaactcgacg ctttgggcaa
      781 agccctgaaa cagcccgcgc gcccgatggt tgccatcgtt gccggcagca aagtgtccac
      841 caagctgacc atcctcgaat cgctggcgga taaagttgac caactcatcg tcggcggcgg
      901 tatcgccaac accttcctgt tggcggaagg taaagccatc ggcaaatctt tggcggaaca
      961 cgatttggtg gaagaatcca aaaaaattat ggcgaaaatg gcggccaaag gcggttctgt
     1021 gccgctgccg accgatgtcg tcgttgccaa agcctttgcc gccgatgccg aagcggtcgt
     1081 gaaagacatt gccgacgttg ccgaagacga tatgattttg gacatcggtc cgaaatccgc
     1141 cgccgagctt gccgaactgc tcaaagccgc aggcacggtc gtttggaacg gccctgtcgg
     1201 cgtgtttgag tttgaccaat tcgcaggcgg cacgaaagct cttgccgaag ccattgccca
     1261 aagcaaagca ttctcgattg caggcggtgg cgacacgctg gcggcgattg ctaaattcgg
     1321 cgttaccgac caaatcggct atatttctac cggcggaggc gcgtttcttg agttcttgga
     1381 aggtaaagag ttgcctgccg tagccgcttt ggaaaaacgc ggcgcgtaag cggtttgata
     1441 taaaaaatgc cgtctgaaac gttgtgctgt ttctgacggc atttttgtgt ggcgattatt
     1501 gtgctttggc tgcccattcc gccggagtgg cggcaaccga aatggaaagt gcgtgcagtt
     1561 cgttactttc cagttgggct ttgagtccgt ctttaatcag gcggtggcgc gcgaggcgtg
     1621 ccttgccttc aaattctgat gaaacgatga cggcgaaaaa atggtgtccg tcgccttcta
     1681 ctttgatatg ttcgcatttt gccacgtctt caatcatggc ttttacttgt tccggggtca
     1741 gcatggtagg ttccttgttg gaaaaatgcg ccattatact ggcaaattga aatgccgtct
     1801 gaaacgggtt cagacggcat ttttgacggt gttttatttt ttggctttga agccgagcag
     1861 gtgctgggtg gtggcaagcc acgcgccgaa tacgcccaac gcgatgacga agccgaacac
     1921 cagcacgatt tcttcagggt aaaagaaacg ccagccgatg ttcaagccgt agggtttgaa
     1981 aatggcatcg accaatgggc gcacggcaga gagcagccaa ccgcaaagcc ccaagctgac
     2041 ggcggcggaa aggatgctct gccacattgc ctgataaagg aaggggcggc ggataaacga
     2101 cgcgggcgcg cccaagagtt tggtgatttc gatttcttct tttcggctga ggatttgtag
     2161 gcggatggtg ttgtgcgcga caaggacgaa cgccatcccc agcgtcaggg aaagaaacca
     2221 taaaattttg cggatgaact cgttgatttg gtacagcgtt tgcacccatt cggtatccat
     2281 agacgcggat tcgaccatag gcagtttggt aatgtctcgg tagattgcct gcatttgggc
     2341 gggcgtggtt gccgggtcgg gggtaacgat aaagacatcc ggcagggggt tgccgtcaag
     2401 catggaaatc agattttggt cgagattgga ctgtaattct tccagaccgt cttctttgcc
     2461 gatgaagcgg atgttgtcga gccgtttgtc gcgcgccagc aggctgcgca cggtatcgct
     2521 gtcgctttgt gcggcggcgg tttccatata gattgtgatt tgcggcgact cgttgagttt
     2581 gcccaacacg ctttgcccgc tttggatgcc caaatacata aacagcggca gggtcatggc
     2641 gacggcgagc attatgaggg taagcagtgt gccgaaaggt tggcgcagaa gctgtttgag
     2701 cgcggtgcgc gcggattcga cgtgcagcga gaggtagtgg atgatgctca tgcgagtcgt
     2761 cctttcgaga ggcgcaggat gcggtgtccg tagtccgcca tcagggtttc gtcatgtgcg
     2821 gcgacgatga cggttgttcc cgcttcgtgg aaggttttga acaattccat aatatcgagc
     2881 gcgtaggcgc ggtcgaggtt ggcggagggt tcgtcggcaa tcagcaggcc gggctggtga
     2941 acgacggcgc gggcgatgca caggcgttgt tgttcaccgc cggagagggt tacgggatcg
     3001 tccaattctc gtcctttcag gccgactttt tcgatggcga tgcgggcacg ctcttcggct
     3061 ttgcgcggcg gatagccgat aatccgaagc ggcaggatga cgttttgcag gacgttgcgg
     3121 tcgtagagga ttttgtggtc ttggaacacg atgccgatgt gttggcgcat aaagccgatt
     3181 tggttgtcgg acaatgtgcc gaggtcctgc ccgttaaaca ggattttgcc cctgcttggt
     3241 ttggtaatgc ccgaaatcag cttgaggacg gtggatttgc ccgaaccgga gtgtcccgcg
     3301 ataaatatca tttcgccttt gttgatttgg aagctgacgt ttttcagggc ttcaaaaccg
     3361 ccgggatagg ttttggaaac ttgttcgaaa cggatcatag gaggtcctgt aaggaatggg
     3421 cggcatatcg tttcgggcgg ttgcgtccgc agggatatgc cgtctgaaca ataggtaaga
     3481 cagtgccgat tataaagatt ccgcctgttt ttagatagtg gataagtttg gcggataccg
     3541 aaggtcgggc gggaaaaatg ccgtctgaag cggattcaga cggcatccgg cgtttaacgg
     3601 cattttgaat gggcgagttt gacggcttcg gtcaggcttt tttcgttcac ttccccggta
     3661 atggtctgcc tgtatccgca tttcggtgct tcgacgacgg taaagggcag tacgccgaca
     3721 gtgtttccgt aggttttcat aaagtttcgg ctgttcgccc cagtgtaacg ccaaatcggg
     3781 taagaaacag gggtttgctt gaggaagttg ccgatattgt cggatgtgtc gagcgcgatg
     3841 ccgaccatat cgacgctgcc ttttttctgc gctttgtacc atttggacat ggcaggcatc
     3901 tcttttcggc acggaccgca ccaggtcgcc caaaggttga cgatgcggac gggggctttg
     3961 agcgattgca ggctttgcgc ggcaccgtct ttccatccgg ccagttcgtc tgccgaggcg
     4021 gtatgtgcgg caaatgtggc gccaagggtg attgcggcaa gattcaagta tttcatcgtt
     4081 ttgtcctttg tatgtcgatt cgggtattgt aaaccgtttg gtacggttgt aggcgggata
     4141 ttgagtaaaa ccctgtgtaa gccgataata aggggattgt cgagaagtaa ggaaatgtca
     4201 tgtctgaaat caaaattttc cacaatccgc gttgcagcaa atcgcgtgcc gccttgtccc
     4261 tattggaaga acgcggcatt gctgccgaag tggtcaaata tttggatacg ccgcccgact
     4321 tgtccgaatt gaaggatatt ttcaacaaat tgggcttgga gtcggcgcgc gggatgatgc
     4381 gtgtgaaaga tgatttgtac aaggaattgg gtttggacaa ccccgatttg gacaataacg
     4441 cactgctgcg tgccatcgcc gatcatcccg ccctgttgga gcgtccgatt gttttggcaa
     4501 acggtaaggc ggctgtcggc aggccgttgg aaaatatcga agcggtattg tgaacaaacg
     4561 gctgttttgc agccgcaacg gcttgcgcta ttacctgttg ggcggtttct gtttgagtgt
     4621 ttttccgctt ttgctggtgt ttacctcatc cgtttgggcg gtttatcgga cgggcgggca
     4681 ggttttgccg ccgtatgtcc gggcggatgc cgcgctggtg ttgggtgccg ccgcatggga
     4741 caaacgccct tcccctgttt ttcgggaacg catcaaccac gccatcgcgc tttaccaaag
     4801 ccgcagggta ggcaaaatcg tgtttacggg agggcgaacc aaaaaaggct atatgaccga
     4861 agccgaggtc gggcggcggt acgcgctcaa acagggggtt ccggcgcgca acatcctgtt
     4921 tgaaaacact tcgcgcaaca cttatgaaaa cctgaacaac atccgtccgg ttttgcgtgc
     4981 caacggtatt gccagcgtgg tgattgtcag cgatccctac catttggcgc gggcggcgga
     5041 aatggcggag gatttgggtg tccgcgttta tatgtctgcc accccgacca cgcggtttga
     5101 tgcgggaaat aagaaaaaaa tctttatgct gcaagaggga tatgcgttat ccctctaccg
     5161 tttggaaaaa tggggcagcc gctttttggg atggctgtct gattgaagcg gtagaaaatg
     5221 ccgtctgaaa cttatccgca ggcagggttc ggacggcatt tttaatgccc aagcacggta
     5281 aaaacagtaa aataagccgt tttaaagatt ttggagccat catgaccgtc aaaacccgtt
     5341 tcgcccccag cccaaccggc tacctgcaca tcggcggcgt acgcaccgcc ctgttttctt
     5401 gggcgtttgc ccgccatcat aaaggcgaat tcttattgcg catcgaagac accgacttgg
     5461 cgcgctctac tgccgaatcc gtcaacatca tcctcgacgg catgaaatgg gtcggactcg
     5521 attacgacaa cgccggcaac gtcgtgtacc aaacccgccg tttcgaccgc tataaagaag
     5581 tcatcgccga acttttagaa aaaggcgatg cctactactg ctattgcagc aaagaagagt
     5641 tggaagccat gcgcgagaaa gccgaaaaag aaggcacggc gacttacgac cgccgctggc
     5701 ggccggaagc aggcaaaacc ctgcccgaaa tccctgccgg cgtgcaaccc gtcgtccgct
     5761 tcaaaacgcc tttggatggc gtaaccaagt gggcggactt ggtcaaaggc gaaatttcca
     5821 tccccaacga agccctcgac gacctgatta tcgcacgcgc cgacggcaca ccgacctaca
     5881 acttctgcgt cgtggtggac gactacgaca tgggcgttac ccacgttatc cggggcgacg
     5941 accatgtgaa caacaccccg aaacaaatca atatcttaaa agccatcggc gctaccctgc
     6001 ctgaatacgg ccacctgccg atgattctta acgaacaagg caaaaaaatc tccaaacgca
     6061 gcggcgatac cgttgccatt accgatttcg gcgcgatggg catcctgccc gaagccatgc
     6121 tcaactatct ggcacgcttg ggctgggcgc acggcgacga cgagttcttt acgatggaac
     6181 aattcatcga atggtttgat ttgaaagacg tttccccgtc cccaagccgt atggatttga
     6241 agaaactcta ctggattaac ggcgaacaca tcaaaatcac cgccaacggc aaactcgccg
     6301 aactcgtcaa accccgcctt gcgctgcgcg atattcatga aaccgaaaaa cctgcgttgg
     6361 aagacgtatt ggcactggtc aaagaccgcg cccaagattt gaacgccttg gcagacgaat
     6421 gtctgtattt ctacaaaaaa caagtccctg ccgaagccga tgttgccaaa cactgggacg
     6481 acgaagccgc cgcccgtatg ctgcgctttg ccgaacgcct cgaagggctg gaagactgga
     6541 atgccgaagc catccacgac ctcttcaaac ccttctgcga cgaagaaggc attaaaatgg
     6601 gcaaactcgg catgcccctg cgccttgccg tctgcggtac ggcgaaaacc cccagcgtcg
     6661 atgctgtgtt ggcattaatc ggcaaagaag aagtgttgaa acgcatccgc gcttaagtta
     6721 tccacaggtt cgggtaatgc cgtctgatgt tggaatacgg cttcagacgg catttaaatg
     6781 gaagttaaaa atgaaaaaaa tattttattt tctgatggtt gttttttcta ccagtgtatg
     6841 ggcaggggat gctgaagaca atttgctcag tatccaatcc ggttaccgcg ccttattgca
     6901 aaaacaaaac aagcttgacg gaaaaattat cgggatgcag tcggatttgg aagatgcgcg
     6961 cagacggttg caggcggctc aggcagacat cgcccgtttg gaagcggaaa ttcctgcagc
     7021 aatggcgcaa aaagcccggc aggctgaaga gctgaggcaa atcggagtgc gtttggacca
     7081 tgcttggaat gcggtttacg gcgcaggggg aacgaaagcg tcggggaatt gaccgatgaa
     7141 ttccctcttt gtggacaata ctgttttcat tacacggctg aaagccgggc atatcggcag
     7201 gttggttcag gcgttgtttg aggagtggca cggatttgaa ccgtggtctt ctgtggataa
     7261 gattcgtgcc tattacggca ggtgtttgaa ggatgacgaa ctgccgctgg cttttgcggc
     7321 tgtggatgat ttcggaattc tgttgggttc ggctacggtc aagcggcatg atatggaaag
     7381 ttttccacag tatgaatatt ggttggggga tgtctttgtt ttacctgaat atcgcggaaa
     7441 aggcattggc aggaggttgg tcgcccactg cataggcgca gcgcgttcgc tggggataaa
     7501 gttcttgtat ctttatacgc ctgatgtgca aatattttat gaatcattcg gctgggcggt
     7561 tgtcgggcga catttccata acggtgaatg ggttacggtt atgcgtttgg atatagataa
     7621 ggtttaagcc gaatggacaa atgccgtctg agctgttcgg acggcatttt ttatatttgg
     7681 aaatttcctg tcagataatg tttatccaca gatttttcca tatggtttga tgctgttccg
     7741 acaacggaaa ttaagatacg gatttgcaca aatctttcag acggtacagt tctgacaatg
     7801 cttcgcgcgg ggtcaggctg tcgggatcga gtttttccaa ggctgctgcc aatatacctt
     7861 caaaatgttt ttcctctgct ttatccacaa agttgcccac attcggttca tctccttttt
     7921 cagacggcat ggtactgaaa atatccagtt ggggacggtt tgcggcggct tggtcttcca
     7981 gttcgttcaa atgcttttgg gcggatttca atgcgcgtac aggcaggccg gcgagtttgg
     8041 cgacggcgat gccgtagctt tttccggcgg gccccggttg gatttggtgc aggaacacga
     8101 tgtcctgtcc ctgttcgagc gcggaaaggt gcatattgac ggcggcggcg tgggtttcgg
     8161 gcaggtaggt cagctcgaaa tagtgggtgg caaacaggct gaaggatttg tttttttgca
     8221 gcaggtgttc ggcaacggcg tgcgcgaggg cgaggccgtc gaaagtggaa gtaccacgtc
     8281 cgacttcgtc cattaaaaca aggctttgtt cggtggcgtg atgcaggatg taggcggttt
     8341 cgctcatttc gaccatgaag gtggagcggt tggaggcgag atcgtccgat gcgccgatgc
     8401 gggtgaagat ttgatcgatg ggtccgattg tggcggcatc ggcaggcaca aaacagccgg
     8461 tgtgtgccaa taaaacaatc agcgcgactt ggcgcatgta ggtggatttg ccgcccatat
     8521 tggggccggt gagcagcatg aggcggtgtt tgtggtcgag gtcggtgtgg ttggcggtga
     8581 agtggcgtac ctgctgttcg acaacgggat ggcgaccgtt ttcgatgtgg ataaccggat
     8641 agtcggcaaa ctcggggcgg acgaagttcc gctcttttgc caaggctgaa aatgtggaca
     8701 acacgtccag cgcggcggcg gctttggcgg ctttttgaag ctgcggcaat gccgtctgaa
     8761 ggtttttcaa tacgccgtca aagagttgtt tttctaaggc gagggcttgc tcttgagcag
     8821 tcagcacttt gtcttcaaag gctttcaact ccggcgtgat gaagcgttcg gcgtttttga
     8881 gggtttgccg gcgttggtag tcggcaggtg cttgttcggc ttgggttttg gacaattcaa
     8941 tgtaaaagcc gtgaacgcgg ttgaactcga ctttgagtgt ggacaaaccg gtacgttcgc
     9001 gttccttggc ttccaaatcc agcaaaaatt cgtcgccatg gttttgaatg cggcgcaatt
     9061 cgtccagttc gggatgaaaa ccgtggttga tgacattgcc gtctttcagc cagacggaag
     9121 gttcgggcaa aatggcttgg cggagctgtt cggctgtgga taggttttcc gggaaaacgg
     9181 ctttgagggt ttccaacagg ctgctgccgt ttgcggacaa gtcgatttgt gccaactcga
     9241 acagactgtc gcgtaaagat gccaaatcgc gcgggcgggc gttgccgacg gcgatgcggg
     9301 cagcaatgcg ttcaatgtcc gcaatgtttt tcagacggca ttggaggggt ttgtattggc
     9361 tttccagcgc ggcaacggct tcttggcgcg ctcggatgtg ggcgcggttg cgtaaagggt
     9421 ggtgcagcca gagagccaag aggcggctgc ccatatgggt agcgcaaagg tcgagcgtgg
     9481 acatcagggt cggcgatttt ttgccggaga gggtttgcgt gatttcgaga ttgcggcgcg
     9541 tggcggcatc cataccgata tattggctgt cggtttcgag cgacaggccg tccaaatgtt
     9601 gcggcatcag gttttgcgtc agacggatat agttcaacag tgcacctgcc gcgccaatcg
     9661 caacggcgtg ttctttgccg tccaaaccga agccgcgcaa atcctggcag ccgaaatatt
     9721 ccgtcagcag tttttccccc gcgtcggcgg caaactgcca cgcgttcagg cgcgtaacac
     9781 ccgatgccgt ctgaagttgc ggtgcgtttt tactgtcagg caacagaatt tccgccgcct
     9841 gcaggcgcgc cagttcgtcg tccaatttat ccacagttgt cagcttggtt ttgaattcgc
     9901 cgctttgcag cgatgcccac gccaaaccga tgtatttttt gtcgggggac acggcaacga
     9961 tgcggttggt ttccttgtct tccagcaatg cggaatcggt cagcgtgccg ggcgttacga
    10021 tgcgcacgac tttgcgctcc acaggccctt tgcccgcgcc gacttcgccg acctgttcgc
    10081 aaatcgccac gcttttaccc aacttgacca ggcgcgccag atattgttcg gcggcgtgaa
    10141 acggcacgcc tgccattttg accggctcgc catccacctg tccgcgcgtg gtcagggtaa
    10201 tatccaaaag ttttgccgct tctaccgcat cgtcgaaaaa catctcgtaa aaatcgccca
    10261 tacggtaaaa caccagtttg tcggtatgtt gcgctttgat gccgaggtat tgctgcatca
    10321 ttggggaaac ggcggatttg gacatggggc tgccttatca ttaggaaaac ggtattgtag
    10381 caaaaggttt tgtcttcgac ggaatgagac tgaccgtaag cctgtcaata aggttgtgga
    10441 gacaactagg aaaacagcaa agttatccac agaaatatta ttaagtcaaa aaacatccac
    10501 gcttatctgt cttatacagc aagtcttaaa ctcaaactta tttgacaaaa aggtaatgaa
    10561 aatattggat atttgctact tacgcacaag caggttggca atcatcaaaa tcatcattaa
    10621 aattttaaat tcaaatattg atggtaaaag ttttggaaat ctgtgcaaac agtaaaattc
    10681 atctatatca aataagcggc aagtagttag atgtaatagt ggaatgtaac tttatttcag
    10741 aatacaatac aggtattgaa atttcgtttc acagcttgga gtatatacat gagcattaaa
    10801 gtagcgatta acggtttcgg ccgcatcggc cgcctcgcat tgcgtcaaat tgaaaaagcc
    10861 cacgacatcg aagtcgtcgc cgtcaacgac ctgacccctg ccgaaatgct gctgcacctt
    10921 ttcaaatacg acagcacaca aggccgtttc caaggtactg ccgaattaaa agacgatgcc
    10981 atcgtggtaa acggcaaaga gatcaaagtt ttcgccaatc cgaatcccga agaattgcct
    11041 tggggcgagt tgggtgtgga tgtcgtcctc gaatgtaccg gcttctttac caataaaacc
    11101 aaagccgaag cacacatcag ggccggcgcg cgcaaagtcg tgatttccgc acccggcggc
    11161 aatgatgtga aaaccgtcgt atatggcgta aaccaagaca ttttggacgg cagcgaaacc
    11221 gttatctccg ccgcttcctg caccaccaac tgccttgccc cgatggcggc agtcctgcaa
    11281 aaagagttcg gcgtagtcga aggtctgatg accaccatcc acgcctacac cggcgaccaa
    11341 aacacccttg acgcgccgca ccgcaaaggc gatttgcgcc gcgcccgagc cgccgcgctc
    11401 aacatcgtgc ccaacagcac cggtgccgcc aaggccatcg gtctggttat ccccgaattg
    11461 aacggcaaac tcgacggctc cgcccaacgc gtccctgttg ccaccggctc gctgaccgaa
    11521 ttggtttcca tcctcgaacg ccctgtaacc aaagaagaaa tcaacgccgc gatgaaagca
    11581 gccgccagcg aatcttacgg ctacaacgaa gatcaaatcg tttcttccga cgttgtcggc
    11641 atcgaatacg gttcgctctt cgatgcgacc caaacccgcg tgatgaccgt gggcggcaaa
    11701 caattggtga aaaccgttgc ctggtacgac aacgaaatgt cctacacctg ccaactcgtc
    11761 cgcactttgg aatacttcgc aggcaaaatc taaggcttga acaaacctgt ggataagtaa
    11821 atgccgtctg aacacattgc gttcagacgg cattgtttat ctgtatagtg gattaaattt
    11881 aaaccagtac ggcgttatct cgccttgccg tactatttgt actgtctgcg gcttcgttgc
    11941 cttgtcctga tttaaattta atccgctata acttgatatt gcggcttatg gcgaacaaac
    12001 aacagccgtt tgttcaactg ctatgcggca aaaccggatt tttgcccgat ttaaagcggg
    12061 gttggggaaa gatcaataac ggttgcacgg ttttcctcga tttgaaatct gacttcgtaa
    12121 tctgcaatat tcatcacata aatccgttcg ggaatattct gataggcggg gcgcggatct
    12181 tgggcaatgc tttggctgat aaggtttttg gtgtttgcag ataaattttc cgcgccgatg
    12241 ttttcctgcc aaacgacttc caactctacg ggtttgccgc tgacgaaacc ggatgcggca
    12301 tcgggtttgg attcgacaaa ggggatataa ggtttgatgt ccacaatcgg tgtgccgtcc
    12361 agcaggtctg cgccgctgca atagaggcgg acgggtttgc cggtttcgat gcgttcgagt
    12421 ttcaggagcg agagtccgag atggttgggg cggtgggggc tgcgcgtggc gaacacgccc
    12481 attttttgtt tgccgccgag ccgtggcggg cgcaccattt gcgcccagcc ttcattcaat
    12541 acgccgtgaa aaataaaact tatccacaca taatcgaaat cttccagccc gcgcacgctg
    12601 tctgcggtga atttgggatt cagctcgatg caggcttttg cggcggagac caaaccgggc
    12661 tggcgggcga tgccgaattt ctgtttgtag ggcgagcggg cggtgccgat gggggtaatg
    12721 gtataagtca taaggttgtg gataagtttt ttggaaaaac cttatcttat cacaatcggt
    12781 tttaccgctt ttgggcagta atttgttata atacagggca ttttaatcgc gttgaaataa
    12841 tgagatgatt gtttccattg atgttgatgc acaaaaaacg tttacgccac tgtgtcctga
    12901 cgaactgccc gtgaacgagg ggcatttgat tgtcgaggag ttgaatgcgc aagccgcttt
    12961 ggcggatttg cgcgtgatga cgaaagatgc gcatcatatg gtagcgaaat ggcttgtgga
    13021 taaccctgtt gatatgttga agccgacagg tttgcctgat gcggatttga cttgggtggc
    13081 tcatgcgatg gtcggtacgc gcggctatga attattggac gggctgcctt ctgctaaaga
    13141 atacgattat tgcgtttgga aaggtgttga tcctgaattg catccttacg gcgcgtgttt
    13201 tcacgatatt gaggaaaaac taagcacagg gctgattgaa tggctgcgtt gtcaaaatac
    13261 ggatacggtt attatcggtg ggttagctac ggattattgt gttaaaacaa cggttttgca
    13321 gttactcaaa ggcggtcgtt ggcaggttgt cgtcaatgaa gcggcttgtc ggggtattgc
    13381 gccggaaacc atcgaaacag catggcagga aatgcattct gctggggcaa tcatcttaaa
    13441 aaacgctgat gaaataaata aatatattaa taatcaataa gttaaaattg tttcacgtga
    13501 aacccccttc ctaaatatga aaattttgct tgtccgcttg tctagtatgg gcgatttaat
    13561 tcacactttg cccgcaatcg aagatttggc gcgacaatgt cctgatgtag aactgcattg
    13621 gctatgtgag gctggatttg cagatattgc gcgcctgcat ccgtttgtaa aaaaaatcca
    13681 tgtgatgaaa tggcggcaat ggcgcaaaca tctctttcgg gctgaaactt ggcgggaaat
    13741 gggtcatctg aaacagactt tgcggcagga agtatttgat ttcgtattgg acagtcaagg
    13801 tctgattaaa agcgcgtgtt tcgctaaaat ggcaaaatcc ccgatttgtg gtttggataa
    13861 aaacagtgcg cgcgagggat gggctgcttt ggcgtatgta aaaacatacg ctgtaccgaa
    13921 gggaaaaaat gccgtttggc gcaaccgtga actgtttgcc caagtatttg ggtatgtaat
    13981 gccggaaacg caggtatttg gcttgactgt tcctgaagca ggccgtctga aaaatttagc
    14041 gcagccgtat tatgcggctt tgcatgcgac cagtcgggac agtaagttat ggcctgtgga
    14101 aaactggcgg tcgttgctgc aaaagttgaa tgaagaacag caatgcaata tttacctgcc
    14161 ttggggaaat gaagatgaaa aaacgcgtgc caaacaaatt gcagatgggc tgccgtttgc
    14221 tattgtgtgc gacaaaataa atttattgca ggcagcgtat ctgctgaaat acgcggtcgg
    14281 aattgtcggc gtggataccg gtttgctgca tttggcaaat gccttggaaa aacctgtggt
    14341 cggcatttat accgataccg atccgattaa aacaggcgtt caggtttcgc caattgcaaa
    14401 aaatttgggc aatatcgggc agattccgac cgcagatttg gtttatcaag cgctgatgga
    14461 ttgtgttgca gcagataaag gctaaagggt cgtctaaaac tgatatggcg gcatgatatt
    14521 ttccaattta ttagatacag gaatgtatat ccgctagtct gagtaaatgc tttaaaaatg
    14581 gtataatcgg caagcattat ccgaaagatt tttgaatgaa ttcgttttta aggaaaacgg
    14641 atttggtttt atagggaaga aggtttggtt tcgctggatg gtgaaagcag cgttcttttt
    14701 aggagggttc aatgaaagca ctggtcgcag taaagcgcgt agtggactac aacgtcaaag
    14761 ttcgtgtaaa agccgatggt tcggatgtgg atatcggcaa tgtcaaaatg tcgatgaatc
    14821 cgtttgacga aatcgctgtg gaagaagctg tccgtttgaa agaagccgga aaagtaagcg
    14881 aaatcgtagc ggtttctttg ggtgagaaaa aatgcgaaga aaccttgcgt acagctttgg
    14941 cgatgggtgc cgaccgtgcc attcatgttg aaaccgatac taaactggag tctctggccg
    15001 ttgctaagtt gctgaaagcc gttgcggaca aagaaaatcc gcaaattttc tttttgggca
    15061 aacaagcgat tgatgatgat gccaatcaag tggcgcaaat gctggcagct ttgctgaatg
    15121 cggcgcaagg tacgttcgcg tccaaagtac aaattgaagg cgacgaagtg cagattgtgc
    15181 gggaaatcga tggcggcgaa gaaaccatcg cattgaaact gcctgctgtt atcagcgcgg
    15241 atttgcgttt gaacgagccg cgctttgtca aactccccaa tattatggcg gcaaagaaaa
    15301 aacctttgga aaaactgact cctgacgatt tggttgccga catttcacct cgtttgaaaa
    15361 cggtgaaatt tgccgaacct aaggcgcgtc aggcaggcgt aaaagtagca agcgttgccg
    15421 aattggttga aaaattgaaa aacgaagcca aagtgatttg aggaggctga aatgagtgta
    15481 ttgattattg tcgaacacga caacaaacag ttgaatccta ccactttgca tgctgttacc
    15541 gctgccgcca aactgggcaa agtcgattta ttggttgccg gaaacggcgc atctgctgta
    15601 gtggaattcg cgaagcaagt agcgggggtg aaaaaagttt tggttgcaga tgctgctcat
    15661 tatgccgaag gtttggctga agagttggct ccgctggttg ttaaattggc ggccgattac
    15721 cgctatgttg cagcaacggc aaccacattc ggtaaaaacc ttttgccccg cgtagcagcc
    15781 ttattagacg taccgcaaat ttctgatttg accgaaattg tggataacac gacttttgtg
    15841 cgccccattt atgcgggtaa tgcatttgaa accgtgcaag ttgattcgga aaaattggtg
    15901 ctgaccttcc gtgcgacggc ttttgatgca gctcctgtac aaggtggtca tgccgaggca
    15961 gttaatgttg aagcaacccc tgcccaaaac ctgagtcgtt ttgttaaccg tcagctttcc
    16021 cattccgatc gtcccgaact gactcaggca aaagtgattg tttccggtgg tcgtgcgttg
    16081 ggtagtgcgg aaaaatttaa tgaagtgctg acaccgttgg cggatgtttt gggtgcggca
    16141 atcggtgcat cccgtgcagc agttgatgcc gaatatgcgc caaacgatgc tcaagtcgga
    16201 caaaccggta aagtggttgc gccgcaactc tattttgcaa tcggtatttc aggtgcgatt
    16261 caacacgttg ccggtatgca agacagtaag gtaattgttg caatcaataa agatgctgat
    16321 gcgccgattt tcaatgtagc cgattacgga ttggttggcg atttatttga aatcgttcca
    16381 caattaacag aagcattgaa aaattgatgt ttcacgtgaa actttcagac gaccttgtta
    16441 ttaatgaggt cgtctgaaaa catttcagca tgattacgac ttataaaacc attacctcct
    16501 tgacgcaggc tgagtttaaa gataaaggca gccgttttat tgcatttgcc tatccgattc
    16561 ggacattggc tgatgtgaaa aaatacctcg atccgttaaa ggaagagcat cataaagcac
    16621 gacactggtg ctatgcctat cgtttgggtg tggatggtgt gcaatttcgt gccaacgatg
    16681 atggagagcc atcaggaagt gctggacgac caattttggg acagattgat tcggtgggta
    16741 ttaccgacgt tttggtcgtg gtcgtccgct atttcggtgg tactttgtta ggtgttccag
    16801 gtctgataca tgcgtacaaa gaggcgacgg ctcaagcgtt ggcagttgca gaagtagttg
    16861 aaaagaatat tgaaaaaact gtttggctga aatgtgaata tccgtttttg aatgaagcga
    16921 tacgcattgc taaacaatat caggcggatg tattggagcg ggatttgcag ttggattgcc
    16981 gattgacggt cagtttgtcg ttggtaaatt atgaggcctg tgttgcggct tggaagagta
    17041 cacggcagat tgaagtaaat acggaaaagc cttttgaatg aaaggtcgtc tgaaatgctt
    17101 tgtgatggta tttcagacga cctcttcgct gactaatctt cttgtgtata tttgtctaag
    17161 gcatcgacgc tggagcgcaa atataaccaa gccagttgat ggaattcgcc cagcgcgttc
    17221 catagtgcat cttcactcat aatgctgtat tcgccttctg tgcgcaaatc cacctccgct
    17281 ccatcttcaa tagcccgatg acaagcctga tattcgtggg tataaaaatt gtccatatcg
    17341 cgaatcaaag cgacggcatg tttccaacgg cggatgtctt cttccaattg cggtagtaaa
    17401 tgacaccatt cgcggtattt gctttggatt tcttcaatac gtttttgcat ggttgcttgt
    17461 ccttttttaa tttatgaata agttgaatag gttgcagagt ggaatgaagc ggtcttttgt
    17521 ttgattctac taaaatttct tcagatggga taaaagtccc tttgtatcga gttttcgtta
    17581 aaagtacata tttgtcaagg agatggcatg aaactgctgg ttatcggtaa tggcggtcgc
    17641 gaacacgcgc tggcttggaa attggcgcag tcgcctaaag tggaaacagt atttgttgcg
    17701 tctggtaatg ccggtacagc gattgaaccc aagctgcaaa acatcgactt gactgcgcat
    17761 caggatttga ttgaattctg tcgtaaagaa aatattgttt ttaccgtcgt cggccctgaa
    17821 gcgcctttgg cggcaggtgt tgtggatgat ttccgtgctg caggactaaa aatattcggt
    17881 ccgacacaat atgcggcgca gttggaaagt tccaaagatt tcgccaaagc atttatggcg
    17941 aaatacaata ttccgaccgc gcaatatcaa acctttgaaa acgccgatgc cgcacatgat
    18001 tacgtcaatc agaaaggtgc gcctatcgtt atcaaagccg atggtttggc ggcaggtaaa
    18061 ggcgtgattg tggcgatgac tttggatgaa gcgcatgctg cgattgatga tatgctgctg
    18121 gacaacaaaa tgggcaatgc cggcgcgcgt attgtgattg aagatttcct gcaaggcgaa
    18181 gaagcgagtt ttatcgtcat ggttgatggc aataatgtac tgcctatggc aaccagccaa
    18241 gaccacaagc gtcttttaga cggcgacaaa ggtctgaata cgggcggtat gggtgcgtac
    18301 agccctgcgc ctgtggtaac gcctgttgtg tacgaacgcg cgatgaatga aattattttg
    18361 ccgaccgtag cagggatgaa agcggagggg catgagttca ccggtttcct gtacgcaggt
    18421 ttgatgattg atcaaagcgg tgcaccccat acgattgagt ttaactgccg tttcggcgat
    18481 cctgaaaccc agccgattat gagccgactg aacagcgact tatcggattt ggttgaagcg
    18541 gcaatagacg gcaaactcga tagcgtaacc gcggaatgga gcccgcaaac tgccgtgggc
    18601 gtggtactgg cggcgcaaaa ttaccccgaa acgcccaaaa aaggcgatgt tatttccggt
    18661 ctggatgctg ccaaccaagt aggcaaagtt ttccatgcag gtacaacggc aaacgagaaa
    18721 ggtgacgtat tgaccaacgg cggacgcgta ttgtgtgttg tgggattggg cgataatgtg
    18781 gcgcaagcga aagctaaggc atatggcgcg ttggaaaaaa tcagtttcga cgggatgcaa
    18841 taccgtaaag acattgccga taaagcgatt aaccgttaat tagaaagagg atgatgccgt
    18901 ctgaagccaa gtatgactaa tgtgcttcag acggtatttt atattatgtt atttccgaga
    18961 attatcgcag catcagcgca acgtaagtta agcgtttatt tgaaaaaagg tggtttggtc
    19021 gcttatccga ccgaatcttg ttatggcttg gggtgtcttc cgacattagc caaagcactc
    19081 ggtaagttgg cgcatttgaa aaaaagaccg caacacaagg gtatgattgt catcgggaat
    19141 caatttgaac aattgcagcc tttacttcaa atgccgtctg aaaatcttca ggatatgctt
    19201 agaaaagaat ggcccgcccc taaaacgttc ctattatccg ccaaatcctg tgtcttaccc
    19261 gagttacgcg ggaaacagcg cagcaagttg gcggtcaggg ttcccgccca tgtaggtgcg
    19321 cgccgcttgt gtcaggcttt gcaaacgcct ttggtctcga cttcgtgcaa ccgcgcaggc
    19381 aaacgcgcgt gtcggacgga gagggaagtc agaaggcaat tcggacgcga tgtgtggatt
    19441 gttggtggga gaatcggcag gcagaaatcg ccgagtcaga ttattgacgg ggaaacaggt
    19501 aaacgtttgc gttagatatg ggtacttgaa tgaaaacttt ttttgcatac acaaagaaaa
    19561 aatttttcgg atagcatttc tctacatgat tgttattgca cagagataaa ggtagaagga
    19621 cgcaacgtct gctttaattt tgaggacggt tttgtcgttc cggacaataa tccgaacaat
    19681 caagcgggca agcatctgaa aacagatttt tcccgtgttg ttttaatcca taaaaatgaa
    19741 aacgccgaag atgatta
//
LOCUS       DAWHRS010000015        19503 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWHRS010000015 DAWHRS010000000
VERSION     DAWHRS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19503
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.015"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..291)
                     /locus_tag="WIB82_000404"
     CDS             complement(<1..291)
                     /locus_tag="WIB82_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3841759.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLIIRIDFNLFCRFDELS"
     gene            complement(463..1632)
                     /locus_tag="WIB82_000405"
     CDS             complement(463..1632)
                     /locus_tag="WIB82_000405"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ3841760.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1815..1925)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1978..2874
                     /locus_tag="WIB82_000406"
     CDS             1978..2874
                     /locus_tag="WIB82_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ3841761.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     TEYWIRRFPTQYLFMYNRYKAP"
     gene            complement(2918..3982)
                     /gene="tsaD"
                     /locus_tag="WIB82_000407"
     CDS             complement(2918..3982)
                     /gene="tsaD"
                     /locus_tag="WIB82_000407"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ3841762.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRFEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(4099..5286)
                     /gene="ccsB"
                     /locus_tag="WIB82_000408"
     CDS             complement(4099..5286)
                     /gene="ccsB"
                     /locus_tag="WIB82_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ3841763.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(5279..7231)
                     /locus_tag="WIB82_000409"
     CDS             complement(5279..7231)
                     /locus_tag="WIB82_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ3841764.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQESKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7508..8131)
                     /locus_tag="WIB82_000410"
     CDS             complement(7508..8131)
                     /locus_tag="WIB82_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ3841765.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            8337..8966
                     /locus_tag="WIB82_000411"
     CDS             8337..8966
                     /locus_tag="WIB82_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ3841766.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(9087..11483)
                     /locus_tag="WIB82_000412"
     CDS             complement(9087..11483)
                     /locus_tag="WIB82_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ3841767.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11635..12750
                     /locus_tag="WIB82_000413"
     CDS             11635..12750
                     /locus_tag="WIB82_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ3841768.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12753..13352
                     /locus_tag="WIB82_000414"
     CDS             12753..13352
                     /locus_tag="WIB82_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ3841769.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13353..14000
                     /locus_tag="WIB82_000415"
     CDS             13353..14000
                     /locus_tag="WIB82_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ3841770.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            14018..14563
                     /locus_tag="WIB82_000416"
     CDS             14018..14563
                     /locus_tag="WIB82_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ3841771.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14582..16867
                     /locus_tag="WIB82_000417"
     CDS             14582..16867
                     /locus_tag="WIB82_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ3841772.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
     gene            17769..18281
                     /locus_tag="WIB82_000418"
     CDS             17769..18281
                     /locus_tag="WIB82_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ3841773.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            18361..19440
                     /gene="aroB"
                     /locus_tag="WIB82_000419"
     CDS             18361..19440
                     /gene="aroB"
                     /locus_tag="WIB82_000419"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ3841774.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT         5203 a         5566 c         4510 g         4224 t
ORIGIN      
        1 gctcagttcg tcgaaacggc aaaacaggtt gaaatcgatg cggataatga ggctgtgttc
       61 gagttcggga tcggagaggc tgtgccattg tccgagcagg acggctttga acatggacaa
      121 cagtggatag gcaggacggc cgcggtggtc tcggaggtaa cgggttcttt gacgattcag
      181 gtactgttcg atcggctgcc aatcaatcac ctggtccaac ttcaatagcg ggaagcggtt
      241 gatgtgtttg gcaatcatgg cttgggcggt ttgccggaag aaggtactca tggaaaatcc
      301 cctaaatgtc ttggtgggaa tttaggggat tttggggaat tttgcaaagg tctctatctg
      361 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata taaaaatgcc
      421 gtctgaactg ttcagacggc atttttgatt ttcaaaccgg aattacagcc ccgctgccgc
      481 cctcaatgca gcagctttgt cggtgcgctc ccaagtgaac tcaggttctt cgcggccgaa
      541 atgtccgtaa gcggcggatt tactgtaaat cgggcgcaag agatcgagca tttggacgat
      601 gcctttgggg cgcaggtcga aatgttcgcg aactaaggta atcagttttt cctcgctgat
      661 tttgcctgtg ccgaaagtgt cgatggaaat cgaagtcggt tcggcaacgc cgatggcgta
      721 ggaaacttgg atttggcatt gggttgccaa accggcggcg acgatgtttt tcgcaacgta
      781 gcggcaggcg taagcggcgg aacgatccac tttggacggg tctttgccgg agaacgcgcc
      841 gccgccgtgc ggagccgcgc cgccgtaggt atcaacgatg attttacggc cggtcaaacc
      901 gcagtcgcct tgcgggccgc cgatgacgaa gcggccggtc gggttgatca ggtatttggt
      961 ttcgtcggtc agcagttcag acggcaggac gggcttgata atctgctcaa tgacggcgtt
     1021 tttcaactct tcataaccga tttccgggtc gtgttgcgta gagaggacga cggtatcaat
     1081 gcgttttact ttgccggatt cgctatcgta aaccacggtc agttgggctt ttgcgtcggg
     1141 acgcagccaa ggcaggaggc cgtctttgcg taattcgctt tgacgctgca tcaggcggtg
     1201 gctgtaatag atggcaaacg gcatcagggt cggggtttcg tcgcaggcat agccgaacat
     1261 caaaccttgg tcgccagcgc cttggttcaa gtcaatgcct tcgccttcgt tcacgccttg
     1321 ggcgatgtcc ggagattgct ggtcgtagta cacgccgacc gcgcagccgt tggcatcaaa
     1381 gcccagctcg gaggagttgt agccgatgcg tttgatggtt tcgcgtgcga ctttgatgta
     1441 gtccacttgg gcggtggtgg tgatttcgcc tgccaatacg cacaagcctg tgttgaccaa
     1501 agtttctgcg gcgacacgcg ctttggggtc ttgcgccaag atggcatcca aaatcgcgtc
     1561 ggatacttgg tcggcaactt tgtccggatg gccttcggat accgattcgg aagtaaacag
     1621 atattcgctc attgagattt tccttgaaaa acaaaccatc ttcttcagac ggcatgttgt
     1681 atgaacataa tgtcgacagc gggaaatata gcaaaatttc cctattcata ccattcagtt
     1741 gagaaatatt cccatttgaa tggcactttg gaatctctgc ccgtacgttt cttacaggca
     1801 aaaaaattcc cgcatcaagc gggtttggat tgctctggtg agccacatcg gcttttcaac
     1861 cgtccacctt acttttcctt ttgaaaagca ggttggcatg gaattcccaa ctcttaatgc
     1921 agcgcggatg gtagcagaaa agcctttccg ccgcaatact tccctttttc agacggcatg
     1981 tttcgtttac aattcgggct gtttccccct ttgcgaaccg ccatgcacat cctgttgacc
     2041 gccctgctca aatgcctctc cctgctgcca ctttcctgtc tgcacacgct gggaaaccgg
     2101 ctcggacatc tggcgtttta ccttttaaag gaagaccgcg cgcgcatcgt cgccaatatg
     2161 cgtcaggcag gcatgaatcc cgaccccaaa acagtcaaag ccgtttttgc ggaaacggca
     2221 aaaggcggtt tggaacttgc ccccgcgttt ttcagaaaac cggaagacat agaaacaatg
     2281 ttcaaagcgg tacacggctg ggaacatgtg cagcaggctt tggacaaaca cgaagggctg
     2341 ctattcatca cgccgcacat cggcagctac gatttgggcg gacgctacat cagccagcag
     2401 cttccgttcc cgctgaccgc catgtacaaa ccgccgaaaa tcaaagcgat agacaaaatc
     2461 atgcaggcgg gcagggttcg cggcaaagga aaaaccgcgc ctaccagcat acaaggggtc
     2521 aaacaaatca tcaaagccct gcgttcgggc gaagcaacca tcgtcctgcc cgaccacgtc
     2581 ccctcccctc aagaaggcgg ggaaggcgta tgggtggatt tcttcggcaa acctgcctat
     2641 accatgacgc tggcggcaaa attggcacac gtcaaaggcg tgaaaaccct gtttttctgc
     2701 tgcgaacgcc tgcctggcgg acaaggtttc gatttgcaca tccgccccgt ccaaggggaa
     2761 ttgaacggcg acaaagccca tgatgccgcc gtattcaacc gcaataccga atattggata
     2821 cgccgctttc ctacccagta tctgtttatg tacaaccgct ataaagcacc gtaacgaaaa
     2881 taaaaatgcc gtccgaacaa cttcagacgg cattttgtca tctgacgatt tccgacagcg
     2941 gccagcgcgg acggacattg aacgcaccga cctccctgcc cttgcccagg tgcatcgcac
     3001 cggcaaaggc aatcatggca ccgttgtccg tgcagtatgc cgtcggcggg aaaaacacgc
     3061 tgactttttc ggacggatgt ttcggcttgc ctttgggggt cgggatttgc accgtcatgt
     3121 tgccgaaagt ttcacggagc ttgcggtttg caccgacccc gccggcgacc actacggttc
     3181 tgaaccctgt ctgcaacagg gcttttttca ctttcgccgc caacacatcg actaccgcat
     3241 cttgaaacgc acggcagatg tcgttgcgtg tctgctcagg aatgtcatcc gccccgtttt
     3301 ccgcgcgcac tttctcgacg gcggtcaata cggcggtttt caaacctgaa aaactcatct
     3361 gcaaatcgtc ggaatgaatc atcgggcgcg gaaaaacaaa cgcttcgaac ctgcccgatt
     3421 ccgcaagttc cgacagtttc gcaccgcccg gatacggcaa gcccagcagt ttcgccgttt
     3481 tgtcgaatgc ctcgcccgcc gcatcatcga cgctctcgcc caaaagctcg tagtcgccta
     3541 tgcccctgac cgccataatc tgcgtatgcc cgcccgaaac caacagcgcg acaaaaggaa
     3601 agtcgggttt ttcctccgcc aacagcggcg acagcagatg tccttccaaa tgatggacgg
     3661 ggatgacggg cttgcccaat gccaaagcca gtgcattggc gtagctcgaa cccgccagca
     3721 gcgcaccgcc caaaccaggc ccctgcgtaa aggcaaccgc gtcaatgtcg ccatacgatg
     3781 cgcctgcctg cgccaaacag ccttcagtca acggaacaag gcggcggata tggtcgcggc
     3841 ttgccaattc cggcacaacc ccgccgtatt cggcgtgcat tgccatttga gtgtgcaggc
     3901 agtgcgcccg caatccacgt tccgtatcgt aaagcgcaac acctgtttcg tcgcaagaag
     3961 actcgattcc taataccaac atggtctgat gccgttaaaa actgaaaaac gtattttagc
     4021 ggatttcggc acgactgccg tatcccaaaa acggaacatg ccgtctgaag accgttcaga
     4081 cggcatcgtc gcaccgtatc aaagcgttcc gtaagaatgc agcccgctca aaaacatatt
     4141 cacgccgata aaggcgaatg cggttacgaa caaaccgata atcgcccacc acgccagcac
     4201 tttgccgcgc caaccggcaa ccagccgcaa atgcagccac acggcataat tcaaccaaac
     4261 gataaacgcc cacgtctctt tcgggtccca actccaatag cgtccccaag catccgccgc
     4321 ccacagcgca cccaaaatgg tggcaatggt aaagaacaga aagccgacgg caatcgcctt
     4381 atacataact tcctcaatca acacggattg cggcagccac gatttcccgc cttttccttc
     4441 cgcacgcagg gaaaccagtt cggcaatacc gagcatcgcg gaaatgcaaa acgcgccgta
     4501 accgataaag tttgccggaa cgtggatttt catccaccaa gattgcaatg ccggaatcag
     4561 cggctggatg gtatgcgcct cgcgggacac gctgtaccac aagacaaatc caaccacgac
     4621 cgccataaag ccgaacacga agccgcccaa tttctgtatg gcgaacttac cttcataata
     4681 aagatacatc agcgcggtaa tcaccaaaaa caggatgaac acttcataca ggttggaaac
     4741 cggaatatgc cccgcatcgg gacggagcag atagctttcg tgccaacgta ccagcagacc
     4801 ggtaaagcct gctacggcag acacccatgc aaacacggtt cccataccca acagcgtgtt
     4861 ggtcggcaca ttttttacgc ttgccaaaac cgcgcccgaa atataggcga acagggcgaa
     4921 gaagacaaag gcgcactgcc acataatcgc cgactggctg ctgaggaaat accgcaacag
     4981 gaaaatctct gccgatttga tgttgccgcc atacagaccg acggcggcat aggcaagcaa
     5041 tacgcttaaa ggaacaaacc agcgcatcgg tttgaaaaac caacccaaaa acacggcaat
     5101 accggcactc gcccacaaca tgaccgtttc gtaaatgtcc atatgcatac cggaacgggt
     5161 ctgtacgaaa accgtagccg caaaaaccag cacggcaaat acccaatccc aaagattcag
     5221 attgctgatc aaagacttct gaatcagcag ctcgtgttcc ggaagggttt tatagtgttc
     5281 agtcatgatt caagtccttg ccgagccgtt gcagactctc gacgtgtttt ggaaattcct
     5341 tctgcaaatc ccgttcactg cgggccgaag acatggcaaa acggattttg ccgtctgaaa
     5401 acaataccca cgcccgtttt tcgcgcacat aaaacatcaa taccgtaccc aataccaaca
     5461 gcaccgagcc gagatagacc aaaagcgcac ccggggaacg ggtcatctgc aaacccgacg
     5521 aacgcacctc ggaaaacccg tcaagttgca gcagcatagg cgcgggatat tcggtcaaac
     5581 ccgtgtacgc atccatactg tgcagcagga aacgattccg cgcttcatcc tgctgccatt
     5641 cgggcaagcc gtaccggcgt atggtttcat ccaaagcagc gttcatcacg ccgtaaagca
     5701 tttcgtagaa atagccctgc atcttatcct gctgctcttt cgggatattg gacgtaataa
     5761 attcgtccaa tcccaaatag cctttttgtg caaagatgtt cagcgtgttt tccgcagcca
     5821 gcatgaattg ttcgcggatt tcggcaggtg cgtctttggt tgcgtcggca accagacgtt
     5881 tgcgcccttc cccatctttc aaaaactcac gcaatgccat aaaggtgtcc gctttcaact
     5941 gcttgtccaa ggggatacgc agccagcggt attgctgctg caagccgctg cgcgtgccgg
     6001 taatccaaaa ataatcctgt tcctgcaaaa ccggcagcat atagttttta tattcgaccg
     6061 cctgccctgc cgcatcacgg atacggtaaa caatggaagg gccgatattg gtgtattttt
     6121 tactttcctg agtaacggcg cggacatcgt tcagcgtgga tttcaggctt ttttcccgtt
     6181 ccgcgccctc gctcatgtcc tccacattca tagaagtgaa ctgatcgaac tcaagacgat
     6241 atttgtgttt gccaatttcc aacggaaact ggtgtatgga tgttgccttc aacacgacag
     6301 gctcgcgcga agcatcaccc aaattccacg ccttgaatgt caaatccgaa ccgccgtcgg
     6361 caaaactcgc ctgataaatc gtgatgccgt gcaaggtcaa aggatggttc acgcggatgg
     6421 tgcgctcgag tttctcaccg gttgccttgt ccgtcacttc aatatcgctg gcgaaatcac
     6481 gcggcatacc cgtattgtaa aaatcgatat ggaatttttt cagtttgact tcaaaaggca
     6541 agtcctgaac caatatcccg ttgtcggcat tcaggaaaac cacatccgca ctctgcccct
     6601 cggaaatatt gacgttgccc ctaaatgaga gattggacgc actcaaaata ctttcgggct
     6661 tgaaatcctt ggcataaacc gcctgattgt ccggaacaat ccgaccggtc agcataccca
     6721 gtttcaacag caggttactg tctatcaacc cgcccaggca aatgacaatc aaagcaacat
     6781 gggcaaagat atagccccat ttgttcattg tgcctttttt ggcggcaatc agaaccgacc
     6841 cgtcttcacg gttaatggtt tttccctgaa aaccttgtac ttccagataa cgtttggcaa
     6901 cctcgggcgc aatttttaca tccaacagcg aagaatggcg catcgccgcc agagattttt
     6961 ctttaacctt ttcccgaaaa gacttcattt cgcgccagaa cggcggcaca ttgcgaatca
     7021 ggcacaaact ggtagaaacc accaaaaaca tcatgataac gacaaaccat gccgaagcat
     7081 agacgtcata cagtcccaga aaaccaaaaa tctgcgccca aaaaggtccg aatttgacca
     7141 aataatccgt ctgcggctgg ttttgctgca acaccgtacc gataaccgat gcaataccca
     7201 gcagactgag caaagcgact gcaaagcgca tggagctgaa aaaagcgaac cacggacggg
     7261 aaagaagtgg gggagatcta cgggatttac tcattgtgtg tttattccgc catcaggaat
     7321 atgggaaagc agaattgggc aaacagaaaa caacgtcccg attctactgt cttgatgctt
     7381 tttatttcaa gacaatgaag acagcctgca tcgattccaa cggttgcgat tgaaaaaact
     7441 tatcgcagaa ttgcctgaag ccgtctgaaa acttttcaga cggcctctaa aacagactat
     7501 tgcggaatta acgcaaacct tggataaagt tggcgaccgc tttcaaatct tcttcagaca
     7561 tacggtttgc aatatcttcc atgatggtat ttttacgctg accggacttg taggcattca
     7621 tctgttcaac aatatatgcc tgatgctgac cgcccaaacg cggataagcc tgaatttcgc
     7681 ttccgcctcc cggcataccc gcaccgctcg gaccgtggca ggacatacac gccggcactt
     7741 ttttatcgct caaaccgccg cgatagattt tcgcacccaa ttcgggattt tccttaggat
     7801 tggcttcacc ggatttgggc tgctgtttgg catagaatgc ggatacgttc aaaatatcct
     7861 gatcgctcaa attcattacc accggtttca tcacagctgc cgaaccgtgg gtgcgtttac
     7921 cgtcgcggat gccgatagtt tgatgataga tgtaagcagt atgctgtgcc gccaaacgcg
     7981 gatacatcgc aatgccgctg ttaccgtctg ctgcatggca agccgcacaa accgttgcgg
     8041 caacctgttt gcctttttcc acgtctgctt tgggagaggc ggaaaccgca ccggcagcca
     8101 aaacaaaggc caataaagtc aatcgtttca tggagtgctc ctgattacag cattggataa
     8161 cgcaacaatg ctctttttat attcaaatac gggatttttg acccgattaa aaccgatgat
     8221 tctgtaaacg tgttattcta tactaaattt acattaaatt accactgtgt ttcacataaa
     8281 accaaccgca tatttttgct gtcggacaaa cggcggcgga aaacaaggat atgcccatga
     8341 acctttttca aaacgccaaa ttcttcacga cggtcaacca ccttaaagac ctgcccgaca
     8401 cccctctcga aattgccttt gtcggcagga gcaatgccgg aaaatccagt gccatcaata
     8461 ccctgaccaa ccatgtccgt cttgcctacg tttcaaaaac acccggacgg acgcagcata
     8521 tcaacttctt cgagctgcag aacggcaatt ttatggtcga tttgcccggc tacggttatg
     8581 cccaagtccc tgaagcagta cgcgcacatt gggtcaatct gctcggcgac tatctgcaac
     8641 agcgcaaaca gcttatcggg ctggttttga ttatggatgc ccgccatcct ttaaaagaac
     8701 tcgacatccg tatgctggat tttttccaca cgaccggcag accggttcac atcctgctgt
     8761 caaaagccga caaattatcc aaaaacgaac agataaaaac cctgtcccaa gtcaaaaaac
     8821 tgctcaaacc ttattccgac aggcaaaaca tcagcgtaca gctgttttcc agcctgaaaa
     8881 aacaaggtat tgacgaggcc aaccgaactg tcggaagctg gtttgacgca gcagatgctg
     8941 ccgcttcctc tccagaggaa aactgacccc aattatacgg aaaccgtatt tcccccactt
     9001 gaccgaccgc aaacatttaa aaaattgcca ctgccaaatc taaaatgccg tctgaaagac
     9061 ttttcagacg gcattttgcg gagtctttaa aacagagaat ccaactgctg ctgtttggaa
     9121 ccagtattac tcggaagcac cggcgtttcc tgcatatctt ggcggacttc gtcatccgcc
     9181 gcctgccgtc cgccttctgc cgcgcccccg tcatcttctt ttgcccgtcg ggaaggttgc
     9241 ggcgcaatac cgctgttgtc cagcgtcaag cccggatcgg ttaccatacg ttccttcata
     9301 tagtattcgc cattgctgct gaccacacct tcaggcattt tcatcccctt gccctgcttt
     9361 cctttcaacg caaaacgcat atagtccacc caaaccggca ccgcaatcgt accgccgtag
     9421 ccgacacgcc ccatactctt aggtttgtcg aagccgatat atacggcagt aaccacatca
     9481 gggttaaaac cgacaaacca cgcatcctta ttgtcattgg tcgtacccgt tttaccggca
     9541 atatccgttc ttcccaacgc agctgccccc cttgccgtac cgacacggac cacatcctgc
     9601 ataatcttat acataatata ggcattgcgc ggatcgattg cctgaggcgc attttgccca
     9661 gccaccaaag gttgcatttg ggcgcgcaac ctgccgtctc tgtcataaat cttatcgatt
     9721 acgtgcgaag aaaccctata tccgccgttc gcaaatacgc tatatgcctc cgccactttc
     9781 aacggcgttg tctcgcccgt acctaaagcc atagacaggc ttgccggcag ctcggacgac
     9841 ctgaagccga aacgccggat atactgttgc gcgtaaccga caccgataga catcaaaata
     9901 cggatggaaa ccatattctt ggaagccgtc agagcctgtc tcaaagtaat gtagccggaa
     9961 tatctgccgt ctgaattttt aggtgtccaa accgaaccgt tcggcccttt ccccggcagg
    10021 gaaatcggcg catcgttaac cactgtggac gcggtcatcc ccttagataa tgccgccgaa
    10081 tagacaaacg gcttaaaggt cgaacccggc tgccgcattg cctgaacggc acgattgaat
    10141 gttttgctgt gaaaatcata accgccgacc agcgcgcgca cagctccggt ttttgcatcc
    10201 agcgaaacca aagccccctg cagcaacggc tcttgaacca ccgcccaacg cccgccgttg
    10261 tttttgacac ggatgaccgc gcccctgcgg atacggtcct cccccatttt ttcattattg
    10321 accgcgcggg ccgcaaagcc caaggcgcgc ctatcaagcg taacccgcct gccgccgggc
    10381 agctgtatga cgacattttt ctttttagtc acatccaaca caacggcggg aaccatttta
    10441 tcgacggtat agagtcccga cagatactgg ctgacagtct cctcgacatc ttcactctta
    10501 ctcaaatcga tatagttttc cgcaccgcgg tagctgctgc cgcgatcgaa attccgtaga
    10561 gccttgcgca atgcctcggt tgccaccttc tgatgatcgg cgcggaccgt ggtataaacc
    10621 ttaaaaccct gcgtataggc atcttcaccg tatttctcat acagttcctg acgcaccatt
    10681 tccgccacat ataaagcact ctgatcgatt ttccgaacaa accgctcgta atgcagttcc
    10741 tcattcaacg cctgatcgcg ctgttgcacg gtaatcatct tctcctcgag catattgttc
    10801 aaaatatact tctggcgcaa cttggcacgt tctggattaa caatcggatt ataggcagac
    10861 ggagccttgg gcagtcccgc aagcatggcg gcttccgcca aagtcaaatc tcggacattc
    10921 ttattgaaat agatttgcgc ggcagatgca aaaccatagg cgcgctgacc gaggtaaatc
    10981 tgattgaaat acaactcgag gattttgtct ttgcttaaag actgctcgat tttataggca
    11041 agcaacacct cattgaattt gcgtgtgaac gttttttcac tgctcaaata aaaatttttc
    11101 gccacctgct gcgtaatcgt actcgcaccc gactgcacgc tgccggacac gacattgccg
    11161 acggcagcgc gggcaacacc ccaaacatcc accccccaat gccggtaaaa gcgtttgtcc
    11221 tcggcggcga taaccgcatt ccgcaacacc tctgggaaat cgccgatttt tgtaaattcg
    11281 cgccgctgct ccccatacat accgatgact tccccatccg ccgaataaat agtcaacggc
    11341 attttaggct ggtaatgctg caaagaatcc aaagacggca gtttcggata cgttaccaaa
    11401 atagcaatgg caaccaaacc cactccaaat acacaaaacc caaaaaccaa accaaaacaa
    11461 gtcgttaaaa tctttttaat catagctgaa taataattta ccattattgg tattaaataa
    11521 agtaaaatag caaccgattt ctacaaagca cggtttcaat gtgcaaagaa caaggaatcc
    11581 attacggata ccgaaacggt tactcactgt acaaataaag caggaacttt catcatgcgc
    11641 ttgtttaaaa gcttgaaaaa ccctaaaaaa acagatgcca agctccctaa aaaatcttcg
    11701 ggactcaata accgcgcggc aatcggcatc gatatcgacc agcattccat caaaatggtc
    11761 caattgtcag gacgtagttt aaaccaaatt caattggaaa aatacgtcat tgccaaatta
    11821 ccaaagaata tcattcaagg caataaagtc caaaattacg atcaacttgt tacatatttg
    11881 caacaagcct atgccaaact gggtacttcg tgcaaaaaca tcatcgcgtc cgtcccgcaa
    11941 aatctggcaa ccatcgaaca attgacctac acagacaaag atgcagaatt agacctgcag
    12001 gggttcgtgg agtcctccat ctccgaagtc agctcgatat cgctcgaaga agccaattac
    12061 gactatcagg tcttgtccca atcggccgcc ggcgaagctg tgttggccgt cgcatcgaga
    12121 aaggatgaaa tcgaacccct gattgacgca ttcaacgccg ccggtatgaa attatccgcg
    12181 cttgatgtgg acattttcgg acaatacaac gcctacgcgc tatggataaa ccatttcgcc
    12241 cccgagcttg cagccgaaaa agtcgccatt ttcggcgtat atgccgcaca gacctacgcc
    12301 ttggtcatcc aagacggaaa aatcctatac aaacaggaaa cctccgtcag cgaagaacag
    12361 ctcaaccaac tcatccagcg cacctatcag gtaacagaag aaaaagcgga agaaatcatc
    12421 aactccccgc aaaaaccttc cgattaccaa gaaagcgtgg caaactattt caaccagcag
    12481 attacccaag aaatacaaag ggtcttgcag ttttattaca ccacgcagac cgcagacgat
    12541 atgaccgaca tcaagcatat cctgctgacc ggggaagcgg cgcgccagga aggcatcgcc
    12601 caaaccgtcg cctcacaaac caatgcagat gtacaatgcg tccatcccgc gcgttatttt
    12661 gcggacaacc tcaaaacaga caaacaacaa ttcgaacttg atgcgccgac actgaccagg
    12721 gcgttcggtt tggcggtacg gggattataa ttatgaacaa tttaatcaaa atcaacctcc
    12781 tcccctacag ggaagagatg aacaagcgca aacagcagca gtttaaaacg ctgatgtacg
    12841 gtgccgtgct gacgggcgtt gccgccgttg cggcaaccta cctgtttatc gacaatatga
    12901 tcaataacca gtcggaaaga aacacgctgc tggaaacctc catcgcacac ttggataccg
    12961 agctgtcgga aatacaaaag ctcaaacagg aaaaagatgc cttcctgatt aagaaaaaca
    13021 aaatcgagga gctccagctc aaacgcctcc aagccgcaaa aatcctcgac agcctgaatg
    13081 aggccgtccc cggaagcacc tacctgacct cgctggatgc cgttaccgcc gactcttatc
    13141 ggctcagcgg caggacatcc agcgacaacc gcgttgccgc catgatgagg gcgatgccca
    13201 ataccggcat attcaagcaa cccgaattgt taagcatcaa gaaaaacaat tcgcatcaag
    13261 aatttaccct tcaggcaaca ttacaaccca tcgtaaaggc ggccgaatcc aaagagaatc
    13321 cggcttcggg aaacgcacag gaggcaaact gaatggcttc taaatcatct aaaaccaact
    13381 tggatctcaa caaccttcac ctgctcaacc ttcctgccag gctttttatc gccctgctgg
    13441 ccgttgccgc cgtgctgggg ctcggttatg ccggattgtt caaaagccag atggaatccc
    13501 ttgaggaata cgaagcaaaa gaaaccgaac tgaaaaacac ctacaaacag aaaagtatcg
    13561 acgcggccag cctgaacaac ctgagggacg aacttgcctc aatccgctct gccttcgata
    13621 tcatgttgaa acagctgccg acagatgcag aaattcccaa cctggttcaa gagcttcatc
    13681 aggcaggttc gagcaacggc ctgcgcttgg acagcgttat gccccaacct cccgtagatg
    13741 acggccccat caaaagatta ccctattcca tttccattac cggaaattac gaacagatca
    13801 gccaatttac ccgcgatgtc ggcagcctct cccgaatcat tacccttgag tcgctgaaaa
    13861 tcgcccaatc tccggaaaac ggcggcaatc ctgacggcaa gagcagcatc ctgaacctca
    13921 gcgccattgc caccacctac caagcaaaat ccgtagaaga gcttgccgca gaagcggcac
    13981 aaaatgccga gcaaaaataa cttacgttag ggaaaccatg aaacactatg ccttactcat
    14041 cagctttttg gctctctccg cgtgttccca aggttctgag gacctaaacg aatggatggc
    14101 acaaacgcga cgcgaagcca aagcagaaat catacctttc caagcaccta ccctgccggt
    14161 tgcgccggta tacagcccgc cgcagcttac agggccgaac gcattcgact tccgccgcat
    14221 ggaaaccgac aaaaaagggg aaaatgcccc cgacaccaag cgtattaaag aaacgctgga
    14281 aaaattcagt ttggaaaata tgcgttatgt cggcattttg aagtccggac agaaagtctc
    14341 cggcttcatc gaggctgaag gttatgtcta cactgtcggt gtcggcaact atttgggaca
    14401 aaactacggt agaatcgaaa gcattaccga cgacagcatc gtcctgaacg agctgataga
    14461 agacagcacg ggcaactggg tttcccgtaa agcagaactg ctgttgaatt cttccgacaa
    14521 aaacaccgaa caagcggcag cacctgccgc agaacaaaat taagaagagg attactccat
    14581 tatgaatacc aaactgacaa aaatcatttc tggtctcttt gtcgcaaccg ccgcctttca
    14641 gacagcatcg gcaggaaaca ttacagacat caaagtttcc tccctgccca acaaacagaa
    14701 aatcgtcaaa gtcagctttg acaaagagat tgtcaacccg accggcttcg taacctcctc
    14761 accggcccgc atcgccttgg actttgaaca aaccggcatt tccatggatc aacaggtact
    14821 cgaatatgcc gatcctctgt tgagcaaaat cagtgccgca caaaacagca gccgtgcgcg
    14881 tctggttctg aatctgaaca aaccgggcca atacaatacc gaagtacgcg ggaacaaagt
    14941 ttggatattc attaacgaat cggacgatac cgtgtccgcc cccgcacgcc ccgccgtaaa
    15001 agccgcgcct gccgcaccgg caaaacaaca ggctgccgca ccgtctacca agtccgcagt
    15061 atccgtatcc gaacccttta ccccggcaaa acaacaggct gccgcaccgt ttaccgagtc
    15121 cgtagtatcc gtatccgcac cgttcagccc ggcaaaacaa caggcggcgg catcagcaaa
    15181 acaacaggcg gcagcaccag caaaacaaca ggcggcggca ccggcaaaac aaaccaatat
    15241 cgatttccgc aaagacggca aaaatgccgg cattatcgaa ttggcggcat tgggctttgc
    15301 cgggcagccc gacatcagcc aacagcacga ccacatcatc gttacgctga aaaaccatac
    15361 cctgccgacc acgctccaac gcagtttgga tgtggcagac tttaaaacac cggttcaaaa
    15421 ggttacgctg aaacgcctca ataacgacac ccagctgatt atcacaacag ccggcaactg
    15481 ggaactcgtc aacaaatccg ccgcgcccgg atactttacc ttccaagtcc tgccgaaaaa
    15541 acaaaacctc gagtcaggcg gcgtgaacaa tgcgcccaaa accttcacag gccggaaaat
    15601 ctcccttgac ttccaagatg tcgaaatccg caccatcctg cagattttgg caaaagaatc
    15661 cgggatgaac attgttgcca gcgactccgt caacggcaaa atgaccctct ccctcaaaga
    15721 cgtaccttgg gatcaggctt tggatttggt tatgcaggca cgcaacctcg atatgcgcca
    15781 acaagggaac atcgtcaaca tcgcgccccg cgacgagctg cttgccaaag acaaagcctt
    15841 cttacaggcg gaaaaagaca ttgccgatct gggcgcgctg tattcacaaa acttccaatt
    15901 gaaatacaaa aatgtggaag aattccgcag catcctgcgt ttggacaatg ccgacacaac
    15961 cggaaaccgc aatacgcttg tcagcggcag gggcagcgtg ctgatcgatc ccgccaccaa
    16021 taccctgatt gttaccgata cccgcagcgt catcgaaaaa ttccgcaaac tgattgacga
    16081 attggacgta cccgcgcaac aagtgatgat tgaggcgcgt atcgtcgaag cggcagacgg
    16141 cttctcgcgc gatttgggcg ttaaattcgg cgcgacaggc aagaaaaagc tgaaaaatga
    16201 tacaagcgca ttcggctggg gggtaaactc cggcttcggc ggcgacgata aatggggggc
    16261 cgaaaccaaa atcaacctgc cgattaccgc tgccgcaaac agcatttcgc tggtgcgcgc
    16321 gatttcctcc ggtgccttga atttggaatt gtccgcatcc gaatcgcttt caaaaaccaa
    16381 aacgcttgcc aatccgcgcg tgctgaccca aaaccgcaaa gaggccaaaa tcgaatccgg
    16441 ttacgaaatt cctttcaccg taacctcaat cgcgaacggc ggcagcagca cgaacacgga
    16501 actcaaaaaa gccgtcttgg ggctgaccgt tacgccgaac atcacgcccg acggccaaat
    16561 cattatgacc gtcaaaatca acaaggactc gcctgcgcaa tgtgcctccg gtaatcagac
    16621 gatcctgtgt atttcgacca aaaacctgaa tacgcaggct atggttgaaa acggcggcac
    16681 attgattgtc ggcggtattt atgaagaaga caacggcaat acgctgacca aagtccccct
    16741 gttgggcgac atccccgtta tcggcaacct ctttaaaaca cgcgggaaaa aaaccgaccg
    16801 ccgcgaactg ctgattttca ttaccccgag gattatgggt acggccggca acagcctgcg
    16861 ctattgatgc gtcaaaataa gggcatatgt tttacggcat atgccctttc tttatgcttt
    16921 ttgccgcgac cgaaatgccg tcattcccgc gcaggcggga atccagtccg ttcagtttcg
    16981 gtcagtttcg gtcatttccg ataaattcct gttgcttttc atttctggat tcccactttt
    17041 gtgggaatga cggcggaagg ggtaaatcct cacaacccaa agcctcgtca tttccacaaa
    17101 aaacagcaac ccgaaacagc aacttaaaac cccgtcattc ccgcgcaggc gggaatctag
    17161 gtctgtcggt tcaggaactt atcggataaa acggtttctc caaccctgcg ttctagattc
    17221 ccactttcgt gggaatgacg ggatatgggt ttccgtgcgg acgtgttcgg atttccgcct
    17281 gcgcgggaat gacggcgaca gatgcccaac ggtctttata gtggattaac aaaaatcagg
    17341 acaaggcgac gaagccgcag acagtacaaa tagtacggaa ccgattcact tggtgtttca
    17401 gcaccttaga gaatcgttct ctttgagcca aggcgaggca acgccgtact ggtttttgtt
    17461 aatccactat agtattgata aacatattat cttcaatata ttcaattgga taattgttta
    17521 cctaagcaaa gataattgcc ttttcctgac aaataagtga aatcaacgga ttgtcaaaac
    17581 acagcctgaa ataaaaaacc tccctgattt cttttatttg tccttaaaat cagaaaggtt
    17641 cgggatggtc gggttatttt tccaaacgta ccgccgccct gccgatttcg tataaaattc
    17701 cgccgtaacc cgacaagccc gaaccctgtc gccccgaaag gcggggtgtc aaacattaag
    17761 gaattgtgat gaaaaacttt aacggcaaac tcatcctcat cggactgatg ggcgcgggca
    17821 aaaccacgct gggccggcaa atggcgcagc ggctggatta ccgtttttac gacagcgatc
    17881 acgaaatcgc cgcagccgcc ggcgttccca tccccaccat atttgaaatg gaaggcgaac
    17941 agggattccg ttcgcgcgaa accgccatac tcaaaaagct ggttatcctg ccccatatcg
    18001 tcctgtccac cggcggcggc gcggtgttaa aagaagaaaa ccgcgccctt atccgcaaaa
    18061 gcggcacggt cgtctatctg cacgccccgc ccgaaaccct gctcgaacgc acgcgctgcg
    18121 acaacagccg tcctttgctg caagttgccg atcctttggc gaaattacgt gaactctacg
    18181 ccgcacgcga ccccgtttac cgccaaaccg ccgactttac cgtagaatcg gcaaactgcc
    18241 gggaaaccgt gcaaaccctg ctcaaacgct tatcccgata aaccggcata tgcgccgcgc
    18301 ccagaaaacc aaaccgcgcc ccgcccggcg ggccggcggt tcaaacttta aggaacaaca
    18361 atgaaaacac tgaccgtaca caccccgtcc cacagctacc ccatctttat cggcaacgga
    18421 ctgctgccgc aggcaggaag cctgctcaaa ccgcatttgg gcaaacgcgc cgccatcatc
    18481 gccaacgaaa ccgtcgcccc gctctacctc ggcacgcttc agacggcatt ggatgcggca
    18541 ggcgtatccc atttcagcat catcctgccc gacggcgagg cgcacaaaaa ctggcagacg
    18601 ctcaacctca tctttgacgg gctgatgcaa aaccgcgccg aacgcaaaac cacattaatc
    18661 gcactgggcg gcggcgtgat cggcgacatg gtcggctttg ccgctgccac ctaccagcgc
    18721 ggcgcaccgt tcgttcaaat accgaccacg ctgttgagtc aggtcgactc atcggtgggc
    18781 ggcaaaaccg ccatcaacca cccgctcggc aaaaatatga ttggcgcgtt ttaccagccg
    18841 caggcggtgc ttgccgactt ggacacgctg cacaccctgc ccgcccgcga attgtccgcc
    18901 ggtatggcgg aagtcatcaa atacggcgcg ctcggcgaca tcggcttttt tgaatggctg
    18961 gaacagcata tgcccgaact gatgacgctc gatcgggaaa aactcgccca agccgtgtac
    19021 cgctgctgcc aaatgaaggc agacatcgtc gcccaagacg aaaccgaaca gggcatacgc
    19081 gcatggctca acctcggaca caccttcgga cacgccattg aaaccgagat gggttacggc
    19141 acttggctgc atggagaagc catcgccgcc ggctgcgtgt tggcggcgcg tttgtccgaa
    19201 caactgggca aaacctccgc cgcagatacc gcgcggctcg ccgccctgct cgaagccgcc
    19261 ggactgccgt ccgcgccacc cgtgtttgcc tttgaaaaat ggctggaaca catgagccac
    19321 gataaaaaag tcagcggcgg catcatgcgc tttatcggtc tgaaccggct gggcgaagcc
    19381 aacatcaccg aaattaccga cacggacatc ctccgccgca ccctgcaacc gtatctctga
    19441 tttcctctgc cgatgtgctg ccgcgcgggt ttgacgcacg atgatgattt tccatcatct
    19501 ttc
//
LOCUS       DAWHRS010000016        19459 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWHRS010000016 DAWHRS010000000
VERSION     DAWHRS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19459
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.016"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(103..4272)
                     /locus_tag="WIB82_000420"
     CDS             complement(103..4272)
                     /locus_tag="WIB82_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ3841775.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLERFETGKISVGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIHLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTLWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(4327..6174)
                     /locus_tag="WIB82_000421"
     CDS             complement(4327..6174)
                     /locus_tag="WIB82_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ3841776.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHVYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQVGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKGGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRASVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAESRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6584..7813
                     /gene="sstT"
                     /locus_tag="WIB82_000422"
     CDS             6584..7813
                     /gene="sstT"
                     /locus_tag="WIB82_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ3841777.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            7925..9208
                     /locus_tag="WIB82_000423"
     CDS             7925..9208
                     /locus_tag="WIB82_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3841778.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     LQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSASSSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGN
                     IFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHTENGRPY
                     PTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGR
                     FYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9283..9498)
                     /locus_tag="WIB82_000424"
     CDS             complement(9283..9498)
                     /locus_tag="WIB82_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841779.1"
                     /translation="MFKLGVYTCLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9491..9706)
                     /locus_tag="WIB82_000425"
     CDS             complement(9491..9706)
                     /locus_tag="WIB82_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ3841780.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(9781..11124)
                     /gene="argG"
                     /locus_tag="WIB82_000426"
     CDS             complement(9781..11124)
                     /gene="argG"
                     /locus_tag="WIB82_000426"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ3841781.1"
                     /translation="MSQNHTILQSLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRVKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            11437..12237
                     /locus_tag="WIB82_000427"
     CDS             11437..12237
                     /locus_tag="WIB82_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ3841782.1"
                     /translation="MNAFNLIIIGDEILHGSSQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHSEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12277..13263
                     /locus_tag="WIB82_000428"
     CDS             12277..13263
                     /locus_tag="WIB82_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ3841783.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWESDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            13920..14182
                     /locus_tag="WIB82_000429"
                     /pseudo
     CDS             13920..14182
                     /locus_tag="WIB82_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14220..14594)
                     /locus_tag="WIB82_000430"
     CDS             complement(14220..14594)
                     /locus_tag="WIB82_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841784.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNSRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(14767..15123)
                     /locus_tag="WIB82_000431"
     CDS             complement(14767..15123)
                     /locus_tag="WIB82_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ3841785.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(15266..15610)
                     /locus_tag="WIB82_000432"
     CDS             complement(15266..15610)
                     /locus_tag="WIB82_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841786.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(15769..16170)
                     /locus_tag="WIB82_000433"
     CDS             complement(15769..16170)
                     /locus_tag="WIB82_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841787.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(16174..16914)
                     /locus_tag="WIB82_000434"
                     /pseudo
     CDS             complement(16174..16914)
                     /locus_tag="WIB82_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(16984..17301)
                     /locus_tag="WIB82_000435"
     CDS             complement(16984..17301)
                     /locus_tag="WIB82_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841788.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(17298..17900)
                     /locus_tag="WIB82_000436"
                     /pseudo
     CDS             complement(17298..17900)
                     /locus_tag="WIB82_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(18326..18670)
                     /locus_tag="WIB82_000437"
     CDS             complement(18326..18670)
                     /locus_tag="WIB82_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841789.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(18708..19052)
                     /locus_tag="WIB82_000438"
     CDS             complement(18708..19052)
                     /locus_tag="WIB82_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841790.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(19055..19243)
                     /locus_tag="WIB82_000439"
     CDS             complement(19055..19243)
                     /locus_tag="WIB82_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ3841791.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(19313..19459)
                     /locus_tag="WIB82_000440"
                     /pseudo
     CDS             complement(19313..19459)
                     /locus_tag="WIB82_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
BASE COUNT         4564 a         4637 c         5034 g         5224 t
ORIGIN      
        1 cactatatcg attctgttcc cttgcaggcg gacggcggcg gcggacggtg gaaaagcagg
       61 ttttcaaatg tccggtttgg cggtgcgccg tctgaaaacc gtttatttcc ctttgccgtt
      121 tccggcggag tcttttttgt ccgaaccgaa taggcggtcg aaacgtatgg tgtatgtcag
      181 ctcgccgccc gacgaacggc taccgatacg ggcaaccgcc tgtatggcgc gggtcagccg
      241 gtaaatcagt ttgacggact gttccgcgct ggagatgctg tattcgtagc cgatgtagag
      301 tttgccggtc agttgtttgc cgacggtcag cacctgttcg gcggggttga gttcgccggt
      361 ttgcgcgttg cggctgcgct tgctggtaaa gcccaaatca tccaccagcc cgatgcggtc
      421 gttgatttgc ccggcaagca gcgcgccggc ggctgcggac agggcggcat tgtcgccgct
      481 gctgccgctg ccggcgcggt tgaggatgag ccaggagagc ttgtcttttt cactcatcgg
      541 ttcgtttgcc gtcagcgtaa tgcgcggact gttgaggctg cccaatattt ccacgcccgc
      601 accgacgggg gaaaggcggc gttcggcgcg gatgttgagg ttggggtcgt tgagcgggcc
      661 gacaaaggag accgtgcctt tggtaatgtc caaatcctgc ccgtatgcct tataacgccc
      721 tttgatgacg cggaccgtgc ccacgccccg cacgcttccg cccgattggg cggtcagggt
      781 cagtttgccg cctatggtaa cgtccgcgcc gtagccggcg aagcggatgc cgtcattgag
      841 gtctaaagtc aggttcatat tgacggggag cggtgccgcc gcctcttttt tgacttcgcc
      901 taatacgacg acatcgtcgc cgacggacgg catcgaggat ttttgcgaac cgaacagccc
      961 ctgatccgtt ttaatcatcc cggtaacgga tatgcctttt tgcggcgaat agcgcaggcg
     1021 ggtgttgccg gaaaccgtca ggcggcggtt ggggcgggac aggatgcggt atttgtcgaa
     1081 caccgcgccg atatcgacat cgggtccgct gttttccata ccgaccgtac cggagagttc
     1141 cgccgtccct tcgtgccgga atttcaggct gtcgattacc catttcctgc ccgcgatatg
     1201 cgaacgcagc gagccgttgt ccaagatgat gccttgggtt tggttgcggt aatagagttt
     1261 gtcgccgttg atgctgccgc ccaagtgcgg atcggcgatg ctgccgccga gggttacggc
     1321 ggcattcagg ctgcctttga cggtttgtcc gaccggtagg aagttgcgga atacttcggc
     1381 atcggcaacg gtcaggttga gcctgccgcc caaaggcgcg gtatcgaaag agcggctttg
     1441 cccgacggtg atgttgccgt tgattttccc gtagttgctg ctgccgttga cggcggcatt
     1501 gacggacgga gagcctaccc gtccgccgat ttgcgcggcg gcattcaggc tgccggtaat
     1561 gttttgcgcg gcggcgggca gaaagggctt caatgcgccc aagtcgggaa gggaggcggt
     1621 aatcctgccg ccgagcggtg catttgccat attgccgccg aaggcgttgg cgatgcccaa
     1681 atcggcgtta atccgcccga aacgcgcgcc gccgtcaagc aggattccga tacggtcgtt
     1741 ttgaaagcgc gttttcaggg aaaatgcgtt caaacccaaa gcctgcccgc cgggcaatac
     1801 ggcatcgccg ctttgccggc tgatattgag gtagccgcgc gcgttgcgcc cgtaggcgac
     1861 atcccagtcg ccgtttaaaa ccagattgtg ttcgaagggc ggtttgaaga aattgtgcaa
     1921 ctcggcgata tgcagaccgt gtgcgccgcc ttttgccgat atgccggttt ttttatccca
     1981 agaaaagtgt tgcaggttga ggctgccgcc cattgcctgc caatttgccg cacttgccgc
     2041 cacgcgttcc gcaccggctt cgagcgtcat acggttttgc agcttgaggt tgaatgcgcc
     2101 gccgatgtcg agaatgccga tgctgccctt ccatagggta agttccctgt tgatgccgcc
     2161 tgaagcgtcc aaatcgaatt tgaacggttt gccatccagc gtcatggcgg cgtgtgtgcg
     2221 gatgcggtgc tgcacgcccg tgccgtccag catcaggtcg gcggtatcga caaccgccgc
     2281 tccgcccgac agcgaaaggc ggctgccttt gatgtcggcg cgtatcgggc ggcttgtgtc
     2341 gggcgaacct ttgagcgtga aatcgagcga acggatgtct gccgccttgc cgatgtgcag
     2401 gttgcgcgcc gcgccggaaa ggtcggtttc aaaggttcgg atgccgccgt ccaaatcgcc
     2461 ggaaaggtgt ccgcgtacat ttaaagaccc cgcgagtccg aaaccgaaac gggataaatc
     2521 gggtgcggtg atgttgaggt tgagccggtc gccttttttg ccgaagccgc cgtctgtttt
     2581 aataatgttc cgccccagcc gcaaatcgac ggcggcacgc ggaaggtggc gggactcgta
     2641 aacaatgtcg gcactgccgg caatcggtac gccgttgaac gtgccgggta aaaaccgcat
     2701 tttgcctgtg aatttctctt ttgccagttc gccggcaagg tttattgagc cgttgatatt
     2761 gcctgccgga agttgcggat tgatgcgcga agggtcgaat gcgcgggaac ggatgtccag
     2821 cttgagcagg cggtctttaa acagctcgag atagccttgc gcggtcaggc tgccttgccc
     2881 ggcggcgatg ttgacggtgt cgagcaccag tttccgctgt ccgtttgcgg ggtcgcttgc
     2941 aatggcgagg ctgccgtccg tgcgcgccgt gccgatgccg agttgccaag agattttggg
     3001 cgaggcggtc gtgccaccga tgccgatgct gccgtccaac ctgcctttga acgcggtttg
     3061 cagtacgtct tccgcgccga cggagttgat gcctatattt aaatcgagga tgtctttttc
     3121 ggtgtcgatt ttgcccgaca gcctgatgcc gccccgtccg agcagggcgg cggacgtatt
     3181 gccgatatgc accgtgccgt cctgccggat gacaaagccg cctaaaacct gacggacggg
     3241 gatgccgttg cggtcggcaa agccggcttt ggtgttttcc aaatcgagcg aaccttccag
     3301 cgcgatgccg tctgaaaacg acgggatggc ggtcaggtcg aaattcagtc cggcatcggg
     3361 cagggaaggc acgaaggcgg ccggattgat gttgaaccct ttgaccagta cttcttccaa
     3421 tgttttatcc aatgattcgg caaacgggtg gatgacggat tttcccgaga ggtggatatt
     3481 gccgccgtcg atcgccagtt cggcgcgcac atccttcagg ctgccgctca gccgagccgt
     3541 actgtgtatg gttttgcctt cgagtccgcc tttggtgtaa atggcggtat cgagggcaaa
     3601 cggttttttc aagccgaccg aggctgaccc cgacgaactg ctccacggcg tgtcggcggc
     3661 cttcaggtca aggcggtgtc ctttgcggtc gtaacggtat gccgcgttga ggcgttcgag
     3721 atagacggtt tgtttgtcaa aagttttgcc gacgctgatt ttgcccgtct cgaagcgttc
     3781 gagatagacg gcggcgggca ggtctatgct gtcgggaagg ctgagcggcg ggcgttcttc
     3841 tttaggcgga gtcggtttgg taacgatggc gatgtcgccg gcggaaattt cggtaatgtg
     3901 caggctgcgg cgcatcagtt cggacggttt ccacgcgaag cggaagcggc tgattttaag
     3961 gtctgccccc tcggtttcta tcgaccagtt gtcgccgtcg aagccgtcga gcagcgtgcc
     4021 tttgagattt tgggaggaaa tgtttacacc gaaccaagac gggatttggt acagcccgaa
     4081 gcgcaaaccc gcttccgtac cggcgagcca gccgaggaaa catactgcca aaatcaggac
     4141 agacagcagt gccgccgaca gcttcagcag cgggcggcgt ttttttgccg gcggggtggg
     4201 gcggtgttca gacggcattt tgcgcgtgcc gttttcggtc ggatcggtat ctgtcggtgc
     4261 ggtatcggtc ataatgtctg tttcaaaata gtttgctgca atgccgtctg aagcggccgt
     4321 atcggtttag aagcgtgttc ccaagctgat gtgccagcgg attttcttat cgctgtgccc
     4381 gtaggcgatg tcgaaggaaa acggcgcgag cgggctgaac cagcgcacgc ccagtcccga
     4441 accgtgtttc agcttcatac gtttgaaatt ggcggcggca tcgcccatat cgtggaatac
     4501 cgcgccggaa agggtgcgcg taaacggcag ttggtattcc aggctgccca ccaagagggc
     4561 gcgttcgggc aggaccgatc cgttcgggcc ggcaagcccg atgctgtcga gttcgtaacc
     4621 gcgcacggaa gacgcgccgc cgctgcggaa catcagccct gaaggaacgt tggcattgtc
     4681 gcgggcaacg gtgtaacccg cttgtccgcg tatgatgaac gtgccgagtt ttttgttttc
     4741 gggcgtgaag aaataacctg cgcgggcaga ggtgcggatc agcgcggtgg aggacaggaa
     4801 tgcgcccaaa gtcgtaccga ttttgccgtc gaggtaatgg ccgttttcgg gatgcagcac
     4861 gttgttgagc agctggcgtt tccaagaggc ggtcagcatc gtggcgtggc tgttgcccaa
     4921 atcgatatcc gagccgggga ttttccggct ttctgcgaga aactccgccc ccagcctggc
     4981 atcgatgccc gcgcggtcgc gcacatacca gatgccgccg gagaaggcgc gtttttcgag
     5041 gttttgggtg gtcgaacggt tgtaggaaac gttgcttgtc cagtagttgc cccgatagtt
     5101 gcgcggctgg ctgatgccgg cggcaagcgt ggtttcgtat ttgtccatat cccaaacgac
     5161 cgagccgata tagcctttgt tgaagagatt gtaatagtcg taggcgattt tgccgcccaa
     5221 accgtattcc gaatcgaggc ggatgccggt ttcgagtttg tggcgtttga cctcggttac
     5281 gctgactttg acggggacgc ggtcgccttg gaggcggtcg aagtcggctt gtacggacgc
     5341 gccggaataa tgcccgtttt gttcgagcgc ctgttggaag tcgagcagca ggtcgaggtc
     5401 gtagggcgtg cccggttgga agcgcgccag gccggagacg atttgttcgg ggtaacgctg
     5461 tgtgccggtg atttcaaagt cgccgaaggc gatggggcgg ccgctgtcca cgacgacgtt
     5521 caaatcggcg gtggcggtat cggggttgac ggacgcccgg gtgttgccga gcttggcaag
     5581 cgggtagcct ttgcgcgtta ccgcgccgag gacggaagtt ttgctgtttt cccaactgtc
     5641 ctgatcgaaa tcactgccta ccggctgctg ccagttttcc agcgcgttgc ggtagtattc
     5701 ggcgaggttg ccgtctgaaa ggatgtcgcc gaggatggcg acaccgacgt tggcgatttt
     5761 ggtgcgcggc cccggtgtga tgtgcaccgt ataagccccg cctttttccg tcaggctgac
     5821 tttgctgctg aaatagcctt tgctgcggag catggttttg acgttgtccg gtgcttcttc
     5881 ggcaaggaag cccacctgtt ccttatccaa aacctcttcc tgctgctgcg tgatgagcgg
     5941 caggtgttct tcgaccatat ctttgatttc actgtcctgc gtgtcgatgc ggacggggaa
     6001 tttgggtttt aatttgactg attcggtgtc ggggcttttg tttttgaaca atgcgaaacc
     6061 cgccgccttg ttttcggaaa ggtcggcggc aggcgcgtat acgtgcggaa agaaaaataa
     6121 agccggcagg agcagggcgg tcggtttgat catcatggta cgggtgtcgt gcatcggtgt
     6181 ggcggctttc gattttaaca ctgttcggac ggggtttcga acggctgttt ttgtgttgtt
     6241 cggacggggc gggacacttc agacggcata ctgccgccgg tatttcggag gttgcggccc
     6301 gccggaaatg tatctgcttg ttttaaggta ttttgtttcc aatccgcata tggaaaacag
     6361 gctgcgtttc ggaatagtgc cggaaaatag gcaggattaa ataaatttat tttcgttcaa
     6421 taaattaaac cgttgcgccg aatgataaag caaagcctgt ccggcactgc atagggcaaa
     6481 tcaacgcaac cttaaaacac agccgaaaaa taaaaaaaca cagagaatga gttttctttt
     6541 aaaatacaac attttttaac atatcacaac ataaggaaga attatggcgt tcggcaaatc
     6601 cttatttcat gcgatcggcc gcgtcagcct ggtcaggcag attgccgccg gtttggcgtt
     6661 gggcatcgta atcggttcgg tttccccgca actgggcttg gcggcaggct tgttcggcag
     6721 cctgtttgtc ggcgcgctca aagcggtcgc gccggttttg gtatttattt tggtggcggc
     6781 cacaatcgcg cagcaccaaa aaggcaacaa ggcgcatatc aggccgatta tcgtccttta
     6841 cctcatcggt acgttttccg cagccctgac cgccgtcatc gccggtatgg ttttcccgac
     6901 gcacattgtt ttggcgggcg cgggcgatgt gtccgccgcg ccgccttccg gcattgtgga
     6961 agtattgaaa tcgctgctga tgaacctggt cgccaacccg attaacgcga ttgccaatgc
     7021 caactatatc ggcattttgg cttgggcttt ggttttgggc gcggcgttgc ggaatcacgg
     7081 ttcggacgtt acgcggcagg tcgttgccga tttggcggaa gcggtttcca ccgtcgtgaa
     7141 atggattatc cgttttgccc ctttgggcat tttcgggctg gtgtcgtcca caatcgcgga
     7201 gacgggtttc ggcgcgctgg cggggtatgc gaagctgctt gcggtgctgt tgggctgtat
     7261 ggcgtttatc gcgctggcgg tcaatcccgc catcgtgtgg tggaaaatcc gccgcaaccc
     7321 ttatccgctg gtgtttacct gcctgcgcga aagcggcgtg tatgccttct ttacccgttc
     7381 ttccgccgcc aatatccccg tgaatatggc tttggcgaaa aaactggggc tgcacgaaga
     7441 cacttattcg atttccatcc cgttgggggc gaccgtcaat atgggcggcg cggcgattac
     7501 gattaccgtt ttggctatgg cggcggcgca cacccaaggc atacaggttg attttgccac
     7561 cgcgctgctg ttgagcctgg tggcgacggt cagcgcgtgc ggcgcgtccg gcgtggcggg
     7621 cggttcgctg ctgctgattc cgctggcgtg cagcctgttc ggcatcagca acgatgtcgc
     7681 catgcaggtg gttgccgtcg gctttatcat cggcgtgatt caggattcgg cggaaacggc
     7741 gttgaattct tcaaccgatg ttttgtttac cgctgccgcc gatttgggcc gacagagaaa
     7801 ccgggcggaa taaaccaagc tatgccgtct gaagcccgtt tggcgttcag acggcatatt
     7861 tttacaattt tatgggcata tggcggaaac aataccggct gctttgaata tgaaggggat
     7921 gatgatgttt gaacgcagtg tgattgcaat ggcttgtatt tttgcccttt cagcctgtgg
     7981 gggcggcggt ggcggatcgc ccgatgttaa atcggcggac acgctgtcaa aaccggccgc
     8041 tcctgttgtt gctgaaaaag agacagaggt aaaagaagat gcgccacagg caggttctca
     8101 aggacagggt gcgccatcca cacaaggcag ccaagatatg gcggcagttt cggcagaaaa
     8161 tacaggcaat ggcggtgcgg caacaacgga caaacccaaa aatgaagacg agggaccgca
     8221 aaatgatatg ctgcaaaatt ccgccgaatc cgcaaatcaa acagggaaca accaacccgc
     8281 cgattcttca gattccgccc ccgcgtcaaa ccctgcacct gcgaatggcg gtagcaattt
     8341 tggaagggtt gatttggcta atggcgtttt gattgatggg ccgtcgcaaa atataacgtt
     8401 gacccactgt aaaggcgatt cttgtaatgg tgataattta ttggatgaag aagcaccgtc
     8461 aaaatcagaa tttgaaaatt taaatgagtc tgaacgaatt gagaaatata agaaagatgg
     8521 gaaaagcgat aaatttacta atttggttgc gacagcagtt caagctaatg gaactaacaa
     8581 atatgtcatc atttataaag acaagtccgc ttcatcttca tttgcgcgat tcaggcgttc
     8641 tgcacggtcg aggaggtcgc ttcctgccga gatgccgcta atccccgtca atcaggcgga
     8701 tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca atatcttcgc
     8761 gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgcccg gcggatcgta
     8821 tgccctccgt gtgcaaggcg aaccggcaaa aggcgaaatg cttgctggca cggccgtgta
     8881 caacggcgaa gtgctgcatt ttcatacgga aaacggccgt ccgtacccga ctagaggcag
     8941 gtttgccgca aaagtcgatt tcggcagcaa atctgtggac ggcattatcg acagcggcga
     9001 tgatttgcat atgggtacgc aaaaattcaa agccgccatc gatggaaacg gctttaaggg
     9061 gacttggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggcc cggccggcga
     9121 ggaagtggcg gggaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt
     9181 gtttgccggc aaaaaagagc aggattgatg ctgatgccgc ctgaagcggg ggcgttcaga
     9241 cggcattgtg atgtttgggg tggggtccgg ttcaaatccg catcagttga taaacccgtc
     9301 gtctttcatt ttcttttcgg aaaaatactg gccgaatacc agcagggcgg cgaggatgat
     9361 gacaaacagc cccgccatag tcagtgtgat tttgaagaac aattccgctt cgagaaaaga
     9421 aaaccagagt tgcacgatca gcagcagcac ccagccggca aacagtccga gacaggtata
     9481 aacgccgagc ttaaacacga tcccgctcca aaatgacgac gtaggcctcg cgtttgaaaa
     9541 acagcagcat acggcgcaaa tctttttcca tcgttacaac ccgccagcct tcgggggttt
     9601 gtttgttgag gaacgcgctg aaattgacgg ggttgacctt tgccgcgccg aaaaacaggc
     9661 tggacaacag gctttcctga taaatgacga ctttgtattc tttcatagga ttccttttaa
     9721 cataagggat catcttttca gacgacccct taagttgcta atgatgtaaa acagcaaacc
     9781 ttattgctta ttgcccaact gcggcaatac cgaaccttcg cccagcgaga gcaaaccgct
     9841 ttgcgagtag atacccagtt tgacgcgggt gtcggtgatg tcgaggttgc gcatcgtgag
     9901 ctgtccgatg cggtcgagcg gagtgaacgc agcgtcttcg actttttcca tactcaggcg
     9961 ttcaggttgg taggtcaggt tgggcgattc ggtgttcaga attgagtagt cgttgccgcg
    10021 ccgcagttcg agggtaactt cgccggtaac ggctttggca acccagcgtt gtgcggtttc
    10081 gcgcaacatc agggcttggc tgtcgaacca gcggccttgg tagagcaggc ggccgaggcg
    10141 caggccgttg atgcggtatt gttcgatggt gtcttcgttg tggatgccgg tgaccaaacg
    10201 ctcgtaggcg atgtggaaca acgccatacc cggggcttcg tagatgccgc gcgatttggc
    10261 ttcgatgatg cggttttcga tttggtcgct catacccaag ccgtggcggc cgccgatgcg
    10321 gttggcttcg aggaagagtt cgacgggatc ggcgtattct ttgccgttta gtgcaaccgg
    10381 cacgccttct tcaaagcgta cgctgacttc ttccgggctg acttcgacgt tttcgtccca
    10441 aaaggcaacg cccataatgg gtttgacgat tttgatgccc gagttcaaaa attccaagtc
    10501 tttggcttcg tgggtggcac ccaacatatt ggaatcggtg gagtaggctt tttccaccga
    10561 cattttgtag ttgaagccgt tggcaatcag aaattcgctc atttcgtgac ggccgccgag
    10621 ttcgtcgata aattgctgat cgagccaggg tttgtagatt ttcagcgcgg gattggtgag
    10681 caaaccgtag cggtagaaac gctcgatgtc gttgcctttg taggtgctgc cgtcgcccca
    10741 aatattcaca tcgtcttctt tcattgcgga aacaagcata gtgccggtta cggcgcggcc
    10801 cagaggcgtg gtgttgaaat aggcgatgcc gccggtggaa acgtgaaacg cgccgcattg
    10861 gatggcggcg atgccttcgt gtgccaactg cgcgcggcag tcgattaagc gggcgttttc
    10921 cgcaccgtat tccatcgctt ttttgggaat ggcgttgtag tcgtcttcgt cgggctggcc
    10981 gaggttggca gtgtaggcat aaggcagcgc gcctttgagt ttcatccaca acagcgcggc
    11041 agaggtatca agaccgccgg agaaggcgat gccgactttc tgaccgacgg ggagggattg
    11101 cagaatggta tggttttggc tcataagggt atccttcgga cttgaaaaaa ggagttcatt
    11161 atcttataaa tataacccat tgtcagccgt taaggctgtc tgaaaagaag ctttgcccgt
    11221 ttcgctgaaa atcttttcag acggtcttta cgccatcctc aacctgactc cctcccctgc
    11281 gggggagggt tggggagagg gtacagacag caggccttaa gcaaacgctg cctgaaaaac
    11341 gcaagctcga ccctctccct aaccctctcc cacgggagag ggaacaaatc acagcaccat
    11401 cgacattttc agacggcatc ggtaaagaaa ccatctatga acgcgttcaa cctcatcatc
    11461 atcggcgacg aaatcttaca cggcagcagc caagacaagc atttcgcctt tttcaaatcc
    11521 ctgctcgaat ccaaagggct gaagctcaat caggtgcaat acctgcccga cgaacccgat
    11581 ttgctggtca ggcaactgcg ccgcagcttt tcagacggca tgccaacttt cgttaccggc
    11641 ggtatcggcg ccacgcccga cgaccacacc cgccaagccg cagccgctgc tttggatttg
    11701 cccgtcgtcc gccattctga agccgccaag tttatcgaag gcataaccca gaaacgcggc
    11761 gagccgcttg attcgccgga acacgcccaa cgcctgaaaa tggcagattt tcccgaaggc
    11821 gcggaattgg tgcccaatcc gtttaacaac atcgccggat tttctatccg cgagcattat
    11881 ttcttccccg gctttcctgt gatggcgcac ccgatggcgg aatgggtatt ggaaacttat
    11941 tacgccgacc gcttcaacca aaccgaacgc ggcagccgca gcgtgtatgt gtttgagcag
    12001 cccgaatcgc gcattacgcc gcttatggag catatcgagc aaacttatcc cggcgtgcgt
    12061 tcgtacagcc tgcccagcgt cggttggacg cattcagacg gcacgcaggt caaaccgcac
    12121 attgagttcg gcatcaaagc cgaaggagaa gccgtcaacc ttttggacgc ggcgtgggcg
    12181 gaagtgttgc acagcttgga cggattggga gcagagctga aaaatcgggt aaactgaaaa
    12241 ccgtttgccc aaatcggaca atatttaagg aaaaccatgc aataccgaat cagaagagaa
    12301 aacgaagcac ccgaagcaaa aaatgcgggg gaaaccttat gggagagcga catcatgcgc
    12361 gaagtgctgt tgtccgccta tcaggacagg cgcagggagc ggatgtggaa aaacatctgg
    12421 cgcgccgtca gcaccctgat tctggttgcc ctgattgcag gcattttccg aaaagacgaa
    12481 gccgcattgc agttggcggg caacacgccg cataccgcag tcgtcaatct gtacggcgaa
    12541 atcggcaacg gtgtagaaga ccaggtcaaa aaactcaaag acggtatgga ggccgcctac
    12601 aaaaacccgc aggcaaaagc catcgtcatc cgcgccaaca gccccggcgg ttcgcccgtc
    12661 gtgtccaaca ccgcttttga agaaatacgc cgtctgaagg cgcagcaccc cggcattccc
    12721 gtttatctcg tggcggaaga tatgtgcgcg tccggctgct actacatcgc ggcggcggcg
    12781 gacaaaatct atgccgaccc gtccagcatc gtcggcagca tcggcgttat cggcagcagt
    12841 ttcgacgcga ccggcctgat ggaaaaaatc ggcgtgaaac gcagggttaa aattgcaggc
    12901 agcaacaaag gcatgggcga tccgttttcg cccgaaacgc ccgaacagtc aaaaatctgg
    12961 gaagaaatgc tgaccggcat acacggcgag ttcatcaaag ccgtcaaaac cggacgcggc
    13021 ggccgcctca aattccgaca atatcccgac gtgttcagcg gtcgcgtgta cacaggcgcg
    13081 gatgcgctga aagtcgggct ggtggacgga ctcggcaata tttacagcgt ggcgcgagac
    13141 gtggtcaaag cccccgatgt ggtggattac actccgaagg acgatttcgg cagaatcttg
    13201 ggcagacgct tcggcgcaga actcaaagca agcgtgcgcg aagccctgca ggccgtccgg
    13261 tagcgttgtt tacagtattt ctaagggaat accaaaataa aaatatacac aaaaacactc
    13321 acctccccag tatgctgcca ctaagtcagc cgcgtcacta tttaccgtgc acttaaagcc
    13381 gtaaaaggca ggttgtcccc gggaggtttt gtgcatcctg ataccgatcg gttgttgttg
    13441 ctcaaaggac agaaggccgc tgataaacga gattacctgt ttgtcgctat tgacgatttt
    13501 tatactctgc cattttgccg gacaaaaccg cagacagtgc tgccaagttt ctgaccgaac
    13561 atctgtccga cccctgcttg tacctgattg agtacgttta ctctgacaat gataggtaat
    13621 ataaagagcc gtccaacatg ctttcggtgc agcttgttat gataatggga ttggttggag
    13681 gcttgcccga tttgcttgtc cgcagaccaa cggtaaggcg gagcgggtta tccgtacctt
    13741 gatggagatg tggcatgagg aacagtcgtt tgacagtccg gaacatcggc aaaaggagtt
    13801 gtgctgcttt attaatttag gggctgccct agatcactag ggaaattcaa attaagttag
    13861 aattatccct atgagagaaa gtcgtctaag acggtataaa caaaataaac tcattttgtt
    13921 atcgtggcta tgatgtattg gatgcgggcg aatttagcca tttccgtatc aatcacagca
    13981 cacattttgc cgaacgacaa aaccatatta atggaactga gaacttttgg aatcaagcaa
    14041 aacgtcattt acgcaagttt aacggcattc ccaaagcgca ttttgagctg tatttaaagg
    14101 agtgcgaatg gcgttttaac aacagtgaaa taaaagttct tgttccattt taaaacaatt
    14161 agtaaaatcg agtttatcct agttgtccag ggcagcctca tctctttata gatagctgct
    14221 taccaagtat ccttaattgc taattcaagc caatcttcaa aactattaaa aaattcatgg
    14281 ttttctatat tatctaaatt aatgacaata accctagaat ttcctgttac atcattacca
    14341 tcaaaataag cttcccattc tccattacta gcgaatggga taagatttaa atcaggcagg
    14401 atagaatttc taaatatata agctacttct gccccttctg ttccataatt ctcaaaccac
    14461 cagtgaaaat tatgtggctg aatataaggt attaaagatg tatcgttaga gatacgaaca
    14521 tacttttcag gataacaaaa cctttcagga agcatattac taggatataa attaaattca
    14581 ttttgaataa tcatttcgtt accttaggat cataaatact accattagga aactgggtag
    14641 accaatttgg tggtgctagt tcattttcaa tttgtttttt aattctaaat tgaatagctt
    14701 ctccccatgt ttgcggcatc taatgctgct tgctctgcat caatattact aaaaattgga
    14761 ttagtctcaa aaatgaaaaa ttactgtttt ttcatttttt atagcatatt caagtacatg
    14821 aataatattt ttcactaagg gctctgtaga aaaatctgaa ttttgtaatt tttcttttac
    14881 agccaaaata ctttccttat ctattagcca atccccctca cttggtccaa ataaccaatt
    14941 atcggtaatt tcagaaacaa gtctccataa tccacgattt tgatattcaa taatttgatt
    15001 ttccgtaatt tctacaactg tttcagattg aatgttttta taagcccaat caatcatttc
    15061 agtatattca ctaattgaaa cattgtcttt taaacagaaa attaacaggt tgtgttcatt
    15121 catttgtcta agttcctgat atgagtttgt tgttgtaaat aatcttttgc ttgcttacct
    15181 tgtaaattaa aggattaacc gcaccttgga tgatgccgcg aatatcatcc atacggttac
    15241 cccaccaatt tttattggtg tatcgttaat catttatttc aaaaaagtta tttattatca
    15301 tattatcagg gagcaggtcg ttaaaaaaaa ccccctcaaa ttccattaaa ttagtattta
    15361 tccagcacat aaacgaacca aagcgttctt tccctgagtg taaatattta aaattaaact
    15421 caaaataagt ttttttgctt agaaaattta atggtttttc tatagtgaaa aaaaagccct
    15481 tactcgtttt cgttcgagat actattttaa ataaattttt attttcaata aaataagaat
    15541 attcatttac taagttgtta tgataaaaaa gtaattttat taaaatgttt tcataatttt
    15601 ctaggttcat ttctagctca atttaaaatg atttcattgt tgccgtacca atgcgaaatt
    15661 taccatcaac aaaatttccc ttcactgata caggtacacc atcaacttgg gtagttttat
    15721 tttcaggctg cctgaaagaa agataggcaa cttattactc atcttacgct agccctcaat
    15781 aattgaatac gttgcactag aaccaattat ttctatgaat gtttgctcaa acataaaaat
    15841 atagtattta aagaattctc tattcaaagg aaaataactt tctttcaata tccattccgc
    15901 ataacactca tcaagaagtt caaaaaaaga ttgaccacca ttttcatgaa gcaaagctct
    15961 atctgcatct tccgaaatta aatccataaa agtatctata aaatacgatt catcaacaac
    16021 cctaaaatga ataacatctt caataaaaat ttttatggtt ttatccgtat ttctataacc
    16081 aactgctata acttctactt tatcagagtt tattgctgca ttatcaaaat agaaatagcc
    16141 agttgggaaa tctaagcttt ttaatttcat aatttatcgt gtatttccat atctgatttt
    16201 tatttttcgg ttattactaa ttgttatttc aagtgttggt tctccaccac tactatcatt
    16261 acgagcatta accgcacgcc catcaggtaa attgcccgtt atcgttccat taggacgtgt
    16321 ttgtacattt cgaacattca gactattaaa atcatttagt gcgtcattgt aatcccccac
    16381 tttatcatac tgaacagcgg gaccttttgt tttactcctt tgagtagtat tgctcaaaat
    16441 gtctttgatt tgtaaatcac tgcgcgcctt accggcttct atgcgagctg ccacactact
    16501 cgggctatag gggtcgctat caatcgcagg attaccaccg ctggtttttt gtccgctagc
    16561 ctgtttccca acctgctgta ctgtatcgcc aatttcatct accgtaccta acgccttagt
    16621 ctgcatatgg cgaacagcag gttttttata gccggtattt ttaacccaat cccatttggt
    16681 cgggttaagt tctacttttt tacctctcca aaccgtacct gcggcctctg ctacggcaag
    16741 ggcagtttgg gctgttgctg ttatattcgg atgggcatca gcccattgtc tggcggaatt
    16801 gatgccgtct tttaccgcaa aagcactgtc ctgtaatatg ctcgcggcag caagttgtgc
    16861 ttccgggctt aattttcctg aattattaat accttgtaga gtctgctgcg cagagatgta
    16921 gcaactgtat ttttaccccg tcgggtaaaa atacagttgc tacggctgct gggaaagcag
    16981 ttactatttt cccagtggct caaaattaat tgtttcagca gatattttta taataatccc
    17041 tacatcaata ataatttcac tataaaattg gtatttatat agctctattt cgggtataaa
    17101 caagggaaac aagctatctt ccttatcctc ataatctacg aaattatttg tatctaattt
    17161 aaaattcaaa atattactgc atttgatata tccgaaataa ttaccttcaa ttgtatctgt
    17221 aaaagataat gtaagggtat ttccattatc ggaaaagatt atttcataaa ggcttacatt
    17281 gtgaaaaata ttttttttca tttgacaaaa tatccagaat gatgatgatt atttggcaat
    17341 ggattgcctt cataatcttg tcgttgcgga tattttccct gtacaccatc ggttcgcggc
    17401 actctttggg ttcttataac ctctccattt ggacgaatat ctattttcac gccatcagga
    17461 cctttccatg tttcatattg agccgcatct gaaccagtac gtctaaaacc ttgttcttgc
    17521 aatgcttgac gtaattcatc tcgcgtagtt ggtctgatag atttaacaac tggcttgttt
    17581 cctcctgcca tctcaccgtc caacgtctgc atagggcggg cagcaggtgt tttatagccg
    17641 ttatttttaa cccaatccta tttggtcggg ttaagttcta cttttttacc tctccaaacc
    17701 gtacctgcgg cctctgctac ggcaagggca gtttgggctg ttgcagttat attcggatgg
    17761 gcatcagccc attgtctggc ggaattgata ccgtctttta ccgcaaaagt actgtcctat
    17821 aatgcgcttg cggcagcaag ttgtgctttc ggacttaaat ttcctaaatc attaatacct
    17881 tgtaaagttt gctgcgcaga gatgtaggtc ggatatttgt atccgacaaa aatatttgac
    17941 gcatctatcg tttccgaaat gccgctgttg gaaaatgtcg gattcgagaa tccgacctac
    18001 ggctatttcg tttaagtcat aaatagccat tcatgttcct tctgaatttg cttttgctat
    18061 ccttagaacc aatcatataa acattatcat cattaattaa tgttctaagt ttatgaagcg
    18121 gtcaaatctg tcaaactacc agagatgaag catatcctcc tggaagtaat tctgttcgtg
    18181 ctgtttccat atcttctttg ccatcatcag ttacttctct gtctaaataa aatcttacga
    18241 taagtttttg tctaatttca tcaaattctg ccgtaattaa tcttatattg atatgaatac
    18301 acggttcata cctgctatta gccagtcagg tgtagttgga tggggattca tttttaataa
    18361 tatcttcaag caagtgaaat gtaagaaaag cgtagatact tgaaggccgg ctatctgatt
    18421 ggcaaaaaga aatttcggag ataaagaatt tccgtttata tttttcataa attaaatcaa
    18481 caccttgttt tacataaaaa ctaacttcct caggaaggat atttataaca ttctctcctt
    18541 ttacttttat agttaatggt ttgattttta tgttttcatg aatatctgat aggattattt
    18601 ctaaaaaatt atttttattt ccagtcattc tggctatttt atgtatattt ccattgtgtt
    18661 cgattatcat tttatttttt ccaaggattt ccttctatat tgatagttta tctaaagatt
    18721 tcatttaaaa tatcctgact aatttcttgt ttatataatt ctacaatttt ctgtttctgt
    18781 gtatttgtat aaaatttttc gttggtatct gcttgatatt ttaattgtct caatatttcc
    18841 atgatttcat attctgattt ttcatcatat tcttcaatca tgtaatcaac aaattgtttt
    18901 agaatatcaa aggcttcgta actttccaaa attttaatat gtttatcggc aaaattggac
    18961 atataccatt cgtcagtgat tggattagta tttaaatcat ctaaaaaaac atttaaatca
    19021 tctaaaaaaa catttacatc atctaaaaac atgactaatc cttaaactta aaatctgttt
    19081 tctcaccggt tcttgagttt tcaatgtaat gtatttcaat tttgtttacg ttttgaacac
    19141 gcttaaccca accgtctttt gctaaccaac catttttagt atcggacata ggaggtattc
    19201 tcgcaggagt tttgccctgt gggtttgcct taacttcttc cattgctaat tgttccttta
    19261 aattattagg tattgtacga ccggttgatt tttcaacaga ttgtgtccct atttttaatg
    19321 atttgtttcc cccagccatt tccccgtcta cagtctgcat agggcgggca gcaggttttt
    19381 tatagccggt atttttaacc caatcccatt tagtcgggtt aagttctact tttttaccgc
    19441 gccaaaccgt acctgcggc
//
LOCUS       DAWHRS010000017        19420 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWHRS010000017 DAWHRS010000000
VERSION     DAWHRS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.017"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            196..1605
                     /locus_tag="WIB82_000441"
     CDS             196..1605
                     /locus_tag="WIB82_000441"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ3841792.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFNLMDRDPA
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGEMVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            1651..2445
                     /locus_tag="WIB82_000442"
     CDS             1651..2445
                     /locus_tag="WIB82_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841793.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDAQIRQEAEAGKSDAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            2633..3409
                     /locus_tag="WIB82_000443"
     CDS             2633..3409
                     /locus_tag="WIB82_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ3841794.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            3485..3591
                     /locus_tag="WIB82_000444"
                     /pseudo
     CDS             3485..3591
                     /locus_tag="WIB82_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3662..3805)
                     /locus_tag="WIB82_000445"
     CDS             complement(3662..3805)
                     /locus_tag="WIB82_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841795.1"
                     /translation="MENNKVKSDYAAKQESLGKVDENKNETMKIPPQVGTHMLGFSFI
                     FKC"
     gene            complement(4482..6953)
                     /locus_tag="WIB82_000446"
     CDS             complement(4482..6953)
                     /locus_tag="WIB82_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ3841796.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVREAAVVKLQ
                     AGDVVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRVSSGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LVVLGNAMRLHKQVDMPSEKMPSEQ"
     gene            complement(6950..8128)
                     /gene="hflX"
                     /locus_tag="WIB82_000447"
     CDS             complement(6950..8128)
                     /gene="hflX"
                     /locus_tag="WIB82_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ3841797.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(8133..9413)
                     /locus_tag="WIB82_000448"
     CDS             complement(8133..9413)
                     /locus_tag="WIB82_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ3841798.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(9566..10291)
                     /locus_tag="WIB82_000449"
     CDS             complement(9566..10291)
                     /locus_tag="WIB82_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ3841799.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(10364..11041)
                     /gene="radC"
                     /locus_tag="WIB82_000450"
     CDS             complement(10364..11041)
                     /gene="radC"
                     /locus_tag="WIB82_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ3841800.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(11164..12513)
                     /gene="gshA"
                     /locus_tag="WIB82_000451"
     CDS             complement(11164..12513)
                     /gene="gshA"
                     /locus_tag="WIB82_000451"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ3841801.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFSKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            12773..14182
                     /gene="leuC"
                     /locus_tag="WIB82_000452"
     CDS             12773..14182
                     /gene="leuC"
                     /locus_tag="WIB82_000452"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ3841802.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            14279..14533
                     /locus_tag="WIB82_000453"
     CDS             14279..14533
                     /locus_tag="WIB82_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841803.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            14595..15236
                     /gene="leuD"
                     /locus_tag="WIB82_000454"
     CDS             14595..15236
                     /gene="leuD"
                     /locus_tag="WIB82_000454"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ3841804.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEERVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            15411..15671
                     /gene="dcm"
                     /locus_tag="WIB82_000455"
     CDS             15411..15671
                     /gene="dcm"
                     /locus_tag="WIB82_000455"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3841805.1"
                     /translation="MQQIKFIDLFSEMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFA"
     gene            15717..16232
                     /gene="dcm"
                     /locus_tag="WIB82_000456"
     CDS             15717..16232
                     /gene="dcm"
                     /locus_tag="WIB82_000456"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3841806.1"
                     /translation="MARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETL
                     EALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPT
                     ESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKE
                     RRKKNGLQKSA"
     gene            16247..16681
                     /locus_tag="WIB82_000457"
     CDS             16247..16681
                     /locus_tag="WIB82_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ3841807.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDQNDVAPTLVAMNMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            16697..16858
                     /locus_tag="WIB82_000458"
     CDS             16697..16858
                     /locus_tag="WIB82_000458"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ3841808.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            16859..17428
                     /locus_tag="WIB82_000459"
     CDS             16859..17428
                     /locus_tag="WIB82_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ3841809.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPG
                     FDIADFKMYSDKIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRLGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            17462..18532
                     /gene="leuB"
                     /locus_tag="WIB82_000460"
     CDS             17462..18532
                     /gene="leuB"
                     /locus_tag="WIB82_000460"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ3841810.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLNILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            18695..19258
                     /locus_tag="WIB82_000461"
     CDS             18695..19258
                     /locus_tag="WIB82_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ3841811.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         4838 a         5181 c         4893 g         4508 t
ORIGIN      
        1 gcacccgaac ggatgaccgg caacgatgtg atgcagatgc tggctccgtc cacgcgcaat
       61 caggcacttg ccgccctgac cgccaaaacg gtttccgcct gcttcaaaca cctgtaccgc
      121 taaccttctt cagacggcat gaggcagatg ccgtctgaag ccgttttcct gcaatttcga
      181 cagacgagaa aaatcatgaa atacatcagc acgcgcggcg aaaccgcaca caaaccgttc
      241 agcgaagttt tattgatggg gcttgccccc gacggcggcc tgatgctgcc ggaacattat
      301 ccgcaaatcg ggcgcgaaac cttggacaaa tggcgcggtt tagcttatcc cgaattggcg
      361 tttgaaatta tgcgcctgtt tgttacggat attccggagg acgatttgcg cgacattctg
      421 aaccgtactt acacggaagc ggcgttcggt actaaggaaa tcacccccgt ccgcacgctt
      481 tcagacggca tcaaaatcca agccttgtcc aacggcccga cgctggcgtt caaagatatg
      541 gcaatgcagt ttttgggcaa tgcgtttgaa tatgttttaa acaaagaagg caaaaaactc
      601 aatatcttgg gcgcgaccag cggcgatacg ggttcggctg cggaatatgc cttgcgcggc
      661 aaaaagggcg tgaacgtatt tatgttgtcg cccgacggta aaatgagcgc gttccaacgc
      721 gcgcaaatgt acagcctgca agacgagaat atccacaata tcgccgtgaa gggaatgttt
      781 gacgactgtc aggatattgt gaaggcggtg cagaacgatg ccgcgttcaa ggaaaaatac
      841 catatcggta cggtcaactc gatcaactgg ggacgcatcg tcgcgcaagt ggtttattac
      901 tttgcaggct atttcaacgc cacttcaagc aatgacgaaa ccgtcagctt ctgcgtaccg
      961 agcggcaact tcggcaatgt ttgcgcggga cacattgcca aacaaatggg cctgcctatc
     1021 cgccgcctga ttgtcgcgac caatgaaaac gatgtgttgg acgagttttt caaaaccggt
     1081 gcataccgcc cgcgcaacag cgcgcatact tatgtaacct ccagcccgtc tatggacatt
     1141 tccaaagcgt ccaacttcga gcgtttcgtg ttcaacctga tggatcgcga tcctgcggaa
     1201 atcaatacgc tgtgggcgga agtggctgca ggcaaaggct ttgacctgcg atttgccttg
     1261 gacaaagtcg gtggcaaata cggttttacc tctggcaaat ccacccacgc cgaccgcctc
     1321 gccaccatca agcaggttta cgagcaagac caagaactca tcgacccgca cactgccgac
     1381 ggcgtaaaag tcgcccgcga agtgcgcgaa gaaggtgaaa tggtggtttg tttggaaacc
     1441 gcgttggcgg cgaaattcga tgcgaccata cgcgaagccg tcggcgatgc cgccattccg
     1501 cgccctgccg cgctggaagg tttggaaaac ctgccgcagc gcgtgcaaac cgtgccgaac
     1561 agtgcggatg cggtaaaagg catcatcgaa caaacccttg cctgatattc cgtacagggg
     1621 cattccgtcc gaacttaaga ttgaaagaaa atgaccttac ctatgcagga aacccgtttt
     1681 tccatactgc tagacgaatt ggcggcaaaa caggaggcag ccatcgcccc ccacctgctt
     1741 gccgacggaa ccaaggtatg gatacgcaaa gcaggcaggc acaatgcacg atggcgttac
     1801 gcgctgctcg gtatggttgc ccgatatttg aaactggggg tgttgaagcc ggttcccagc
     1861 ctcggcggcg agcctgccat tgcaaccgaa tcaaaacgcc tgtacgaatt gcgctcggcg
     1921 gggatagccg ttcccgaatt gctcgccgtc aggaaaaatg cccttatgtt cggcaatttg
     1981 gaaggcatcc cgctcgatgc gcaaatccgc caagaagccg aggctggaaa gtcggacgct
     2041 tggcttgccg gtttggaagc cattgcacgc gtgcataaaa aacagcagtt cctcagtcag
     2101 gcgtttgcac gaaatatgat gtgggacgga aaaaacatca gctttttgga ttttgaagac
     2161 gatccttccg aagtcctgac catagcgcaa tgccaagccc gcgattggct ctgctacatt
     2221 cattctaccg cgctaatatt gaaaaacggc ggactgctgg aagctgctgc ggaaaaatgg
     2281 ggcggcgtat tgtcggatca gcctgccgaa atacaaaagc tgattgccgc caccgtcaaa
     2341 ccgatcctcc cgatacgcag gctggaacac ccccgctggg gacgggacgc gctcaggctg
     2401 gcagcctcga tttccctgat ttcccttgcc gatatgccgc cataagccgc cgcccgcccc
     2461 acgatgccgt ctgaagccct tcagacggca tataaccaac gcgcatcaag cattgtcgca
     2521 accatccgtt tcgtttagaa ttttgacttt atcccgaatt gggcagatat tctcattctg
     2581 acacacgtcc caaataaaca ctccccattt cttttaccga caggaaaccc aaatggcagc
     2641 attcaatacc caaaaagtat tgtccgtaca ccactggaca gacgcatatt ttacctttac
     2701 ctgcacccgc gacgaatcgt tgcgctttga aaacggccaa ttcgttatgg tcgggctgat
     2761 ggtggacggc aagccactga tgcgcgcata cagcgtcgcc tccgccaact gggaagaaca
     2821 cctcgaattt ttcagcatta aagtccaaga cggcccgctg accagccgcc tgcaacacct
     2881 caaagtcggc gacgacgtgt taatcagcaa aaaaccgacc ggaaccttgg ttgccggcga
     2941 cctgaatccg ggcaaacacc tttacctgtt gagcaccggt accggcatcg cccctttctt
     3001 gagcatcacc aaagatcccg aaatttacga acagtttgaa aaaatcatcc tcgtacacgg
     3061 cgtgcgctac aaaaaagatt tggcgtacta cgaccgcttt accaaagaat tgcccgaaca
     3121 cgaatacctc ggtgacttgg ttaaagaaaa actgatttac tacccgattg tttcgcgcga
     3181 agaattcgaa caccacggcc gcctgaccga cctgatggtg agcggcaaac tgtttgaaga
     3241 catcggcctg cccaaaatta atcctcaaga cgaccgcgcc atgctgtgcg gcagccccgc
     3301 gatgctgaaa gacacctgca aagttttgga cgatttcgga ctgaccgtct ctccgaaaac
     3361 aggcgtacgc ggcgattacc tgattgagcg cgcatttgtg gaccaataat ctgcttgaaa
     3421 atgccgtctg aacggcatct gcgataaaaa accccgaatc ctgccaatca ggattcgggg
     3481 tttttatagt gaattaaatt taaattagga caaggcggcg agccgcagac agtacaaata
     3541 gtacggcaag gcgagccaac actgtaccgg tttaaattta attcactata tagtgggtaa
     3601 aaattagaaa tgcacatttc cgtcattcac gcgcaagtgg gaatccagac ctatccgtta
     3661 attagcattt gaaaataaaa gaaaaaccaa gcatatgggt tcccacttgc gggggaatct
     3721 tcatcgtctc atttttgttt tcatcaactt ttcctaaact ttcctgtttg gctgcataat
     3781 cagacttgac tttgttattt tccatattga gcttctctta atttgaaaaa attaacataa
     3841 acatttaaaa ataaaacaaa attatataat aaaaaaaata atgagaatat aaaggataaa
     3901 aagagagtta agttatttaa tcggacttgt tctctataaa tcataagaca ccaaataagc
     3961 cataataaat ttagtaaata aaaactgctt atacccaact ttcttttctt ggaacctatt
     4021 attatttcaa ctccggatga aaaaatagaa tttaaaaatt tttttctttg atatatcgtt
     4081 aatatctccc tagtttttct taaaaaaaat ataatcatat atatactaca caacattggt
     4141 aataaataaa ttaaaagcga aaggtaatta aattgataat tgtaaattat ataataaaca
     4201 acatacgaag aaaaaattat tatagatgat ttactatacg aataaaaaaa tatcctacct
     4261 agcccgaacc ttcttataat ttgtactatg tgattatcca tttcaatatc catttaagct
     4321 ttcaattaat tttctatatt tttcaatggc atgtatcatg ctatgcttgg gaaatatcat
     4381 cttggttgat gatcaagcca tgtatgcaaa gattggaata tggtatttgt cgtgctaata
     4441 atgcccccgt tactgttata cgtctaaagc aacgctgtcc gtcattgttc ggacggcatt
     4501 ttttcagacg gcatgtccac ctgcttgtga agccgcatag cattgcccaa tacgaccagc
     4561 gaactgaagc tcatacccag tgcggcaatc cacggttgga catagcccaa aacggcaagc
     4621 ggtacggcaa tgatattgta cgcgcccgcc catatcaggt tttgccggat aatatggcgg
     4681 gtgcgccgcg cctgatcgag caggtgggcg acggtacgca aatcttcgtt caataacaca
     4741 atgtccgcgc cgtccctcgc aatatccgtc ccgcccgctg cggcggcgga tacgtctgcc
     4801 tgcgccaaaa cgggcgcgtc gttgatgccg tcgcctatca tcagcacttt tttcccttct
     4861 ttttgcaagg ctttgacgta ttccagtttg tcctcgggca tggcttgggc gcggtagtgc
     4921 gcgacaccca gggcgcgtgc ggtttcggca acggcggttt cgcggtcgcc gctgaggatg
     4981 tgcagggtca ggtttttgcc tgccaactgc cgcaccgcct ccgccgcgct gtctttcaag
     5041 gggtcggtca ggtagaacac ggcttggaaa ccgctttgac tgccgaggta aaccgcgctg
     5101 ccgccgcctt ctgtttgcgg ttctttacct gaaatttcgg cgacatagga tgccctgccc
     5161 aatgcccaaa cctgtgtttc gccgttgacg gtcagttgcg cgcccacgcc ttcgccgatg
     5221 cggttgaggc gttgtttaat agcgatgtcg gggacgctgc cgcttgaaac acggcagttg
     5281 aggatggcgc gggcaagggg atgttcggac tgttgttcta aagcctgcgc caccgcgaga
     5341 acaaaggctt cgtctgtgcc tctcaacaat gagatacggc ggacggcggg tttgccttgg
     5401 gtcagcgtgc cggttttgtc gaagatgatg tcggtggttt gggcgagggt ttcgattgcc
     5461 tgctttccgc cgattaaaat gccttcgcgc gccagcgtac cggtagaagc tgccagcgcg
     5521 gtcggcgtgg caagcgataa ggcgcagggg caggtaatga ccagcagggc gacggtaatc
     5581 cacaatgcgg tgtgcgcgtc ggcgtacagc gtccagccga tgaagacggg gacggcaagc
     5641 aggagttcgc cgaatatgaa agacgaggcg tattgttccg ccaactcggc agtgcgcggt
     5701 ttttgcgcta aggcgcggtc gagcaggcgg acgatgtgcg acagtcgcgt gccgccgccg
     5761 gtgcggtcgg tgcgtataat cagggggctt tgcgtgttga gtgtgccggc ggttactttt
     5821 tcagacggca ttttggcgac gggcaggctc tcgccggtca gcatagattc gttgacggca
     5881 ctgcttcctt ccagcaccgt gccgtcaacg gggatggttt cgcccggttt gaccagcacc
     5941 acatcgcctg cttggagctt gacgacggcg gcttcgcgga catcctgtac ggcaggatag
     6001 ccgggcatcc ggtggcagaa ggcgggtatc agcttcacca gcctttcggc ggcatcgcct
     6061 gccttgcgtc gggcaatgtg ttccataaag cgtccgccca gcaggaaaaa cagcagcatg
     6121 gctatggatt cgaaatacat cccctgcccc gcattggtcg caaggctgta aacgccggcg
     6181 ataaaggtca tgatgatggc gacggcaatc ggcgtatcca tgccggcgcg gcggtttttc
     6241 aggtcgcgca acgcgccttg ataaaacggg acggcgcaat agaatacgac gggcagcacc
     6301 attaaaaagc cgccccaatg gaggatttgc aggaaatcgg gttcgatgtc gccgccgtaa
     6361 aggtaggtcg gcagcgcgaa catcatcgtc tgcatcatcc ccagcccggc aacggcgagg
     6421 cggacgatgt attgtttgcg ttctttttgg ttggcggctt cgattttttg cgcgtcatag
     6481 ggtgcggcgg tgtagcctat ctgccggatt ttcaacagaa tatcggaaag gcggattttg
     6541 ccgtcgtccc agacgacgcg gcagcggtgc gtgctgtaat tgaggtcgat gcggacgatg
     6601 ccgtctgtac gcaaaagctg ctgttcgatc agccagacgc aggcggcgca ggtgatgccg
     6661 ccgagcatta aaaccgcctc gcgcgtgccg ccgtgggttt ccacaaagtc ggactggact
     6721 tcgggcaggt cgtacaggcg gatttggtcg aggatttctt ggggcggcag ctcggttttt
     6781 tgcgcgtcgg cggtgcgttg tttgtaataa ctgcccaagc ccgcgtcaat aatgctttgt
     6841 gcgaccgcct gacagccggc gcagcaggtt tctcggtctt cgccttcgta gcggacggtc
     6901 agatgcaggt tttcgggaac atccagcccg cagtggaaac aggttttttt catggcattt
     6961 cggtttcgtc tgtgtttggt gcggcggcac aagactcggc aatggcttcg cgcagtgcgt
     7021 ctataccggt attttcagca acggaaatgc ggacggcggc aatttttccc gcagcgtcgc
     7081 gccatatgcc cgtgttttgt tcttcagacg gcagcaggtc ggttttgttg tacaccttga
     7141 tgcacggaat atcgccggca tggatttctt gcagtacgtt ttccacgtct tcaatctgct
     7201 gtccgctgtt cggggcggcg gcatcgacga cgtgcagcag cacatcggct tgcgcggttt
     7261 cttccagcgt ggcggaaaat gccgaaatca gtttgtgcgg caaatcactg acgaatccga
     7321 cggtatcggt caggataatg ctgcattcgg gactgatgta cagccgccgc gccgtggtgt
     7381 cgagtgtggc gaaaagctgg tctttcgcat atatgcccga cttggtcagc cggttgaaca
     7441 ggctggattt gccgacattg gtatagccga ccagcgcaaa cgttttgatt gtgcccgatt
     7501 cgcggggctt gcggcgcagg gcgcgctgtt ttttgaggtt gacaagctgt tttttcaagg
     7561 cattgatccg atgggcgatc aatcggcggt cggtttccag tttggtttcg ccggggcctt
     7621 tcatgccgat accgccgcgc tggctctgca gatggccgta accgcgtatc aagcgtcccg
     7681 ccaaatggct caattgcgcc aactcgactt gcagcctgcc ttcctgcgtg cgggcgcggc
     7741 gggcgaaaat cgccagaatc agccctaccc tgtccaatac gcggcatttc agttcttttt
     7801 caaggttgcg ttcctgcgtg ggcgtgagtt cgtggttgaa tacgaccaaa tcgatgccgt
     7861 ctgcggcaac tgcttctgac agctccgccg ccttgcccgt gccgacaaac agcgcggtgt
     7921 gcggacggtc gcgtttggca gtctccacgc gcacggaatc gccgcccgcc gctttgacca
     7981 gctcgacagc ttccgccaat gccgtctgaa aaccgttcag acgggtggca ctgctgcccg
     8041 tgccatcttt gtccaacatt acgcccacca gcatgacgcg ttcgggttgc gcttgggtac
     8101 tgccgttgcg gcctgttctg cctgtcatgt attcaatcct gtaaataaag ggttacgcct
     8161 cgttccgttt ctccttccgg tgcaagcgtg atgcggctgc ccgccattcc gtcaacgctg
     8221 aagacgatgc cgcccgcctc gtgccttcct atggacaggc ggctgccggg tcgctgccgg
     8281 taggtataac gggtacacgc ttccaaacac cagcgtccgc gccagtggat gaaatatttt
     8341 tcgccttttc taaccgcaag tgtctcgccg ccgtttacct caaaataagg gtaatcgggt
     8401 tttcgggttc taaactgcca tttcgcctgc gcgcgccgtc cgttgcggac atagtcgaat
     8461 accgccgtat aaagcgtacc gtattccaaa ggtttgagcg gaaaaagcgc gaattggtac
     8521 gcggtcagcc tgccgttggg gtcgttgccg gcggttaaaa ccctgacggg gcggatttcg
     8581 tttttaccct gatacagctt gaaacttttc atcgtaattt tgcctgccgc ctcggaaaaa
     8641 tcaatgctgg caggattgcc cgtgatttca tattccggca cggggtctgg acgctcgccg
     8701 tggaaataag gcagtgcgcc gctgccgacg ggataggctg tatagagcag ctcctgtgcg
     8761 ggcatggctt cgtcggtgta cacgaccgca ccgttatggc aggcgttgcg gtaatatttc
     8821 cgtcctgctt ccggctgatt tctgccttgg gcgcaatgcc gctcaaacct gccgttgccc
     8881 tgattgaata cgagaacggt tttaccgttt tcgcgcacaa atgccgctcc tgcctcatcc
     8941 gtatggcggt caagtaggga aaggcggtgg tagattgcgc tcattaatcc gtccacttgg
     9001 cgttgctgcg tgcggatgtc gctgtcggac gattcggcgg cttcctcttc cgtgctgatg
     9061 ttttcatgca cgccgttgta gagatacccg gcaaggcgtg tgcgttcggt cagcttttgt
     9121 gcggtgtagt gcggattgtc gggatggtgt tcgccgtgtc cgtcttcggg attgagcgtg
     9181 agatagcgtg cgtgcctgcg ggcggaattt tccaaaaccg gcgcgtgtgc cagcttgtgc
     9241 aaaccgattt gggcgcggat gcggttgagg tagtgcagcg catcgccgcc gtccctgatt
     9301 tgcggtgcgg acggatagac aagttcgccc gcgggcaggg attggttttg ggtatgatag
     9361 aaaacgcctg ccgccgaacc caacaggagc agccgaataa aaagggattt cataatgccg
     9421 tcctcccgac gcggcaacgg ggtgttttat caaaacggca aacttgccaa tgcggtttcc
     9481 gcttgccgat tcccaaaccg gcaaaccttt ccccgtgccg atgccgaacc cgatgccgtc
     9541 tgaaccttca gacggcatcg ggtgtttatt tgtccacccg gatgggtgca ggttcgtatt
     9601 gtgtcgattc gtcgccgtaa tacagcacgc cgagtttgat ggggatgcgt ccctgcgatt
     9661 tgcggtgggc attggaatcc ctcagggaat aggcgcaacc gcaatattcc tgctggtaga
     9721 agttttcgcg tttgctgatt tcaatcatgc gcgcgctgcc gccgcctttg cgccagttga
     9781 aatcccaata caccacatca tcgtaaggcg cggcggcgcg gtgtccgcag tcgttgattt
     9841 gcgccatatt tttccagcgt gaaatgccca gcgaactggt aaagacgggg aacccgtgtt
     9901 catgcgcgta ttgcgcggct ttttcaaaac gcatatcgaa acacatggtg cagcggatgc
     9961 cgcgttccgg ctcaaactcc atgcctttgg ctttggcaaa ccattctttg cggtcgtttt
    10021 cgtagtcgtc gtctttatcg atgaaaggaa tgccgaactt ttccgcaaag cgcacgtttt
    10081 cctcttttcg gagcatatac tctttatgcg gatggatatt gggattgtaa aaataaatgg
    10141 tgtaatcaat gccgctggcc agcatggctt ccatcacttc gccgctgcac ggggcgcagc
    10201 aggaatgcag caagactttt ttatgtccgc cgggcgggac gagtatgggg cggtcaatgt
    10261 cggtaacggt aggtttgttt tgtgtttcca ttaacttaac aatatctgtc gaatacggtt
    10321 gaagcggcta ttttatcaga gccgccgcat gtaaaacagg gtgtcagggc atcagcccga
    10381 gctgcctgaa cgaacggacg gtttgcgccg ttacgataaa atggtcgagc agcgacacat
    10441 cgaccagcga catggcctgt gccagccgcc ttgtgaacat gatgtcttcc tgcgaaggtt
    10501 cgggcgagcc gcccggatgg ttgtgcgcaa taatcaggct gtcggcatat tcgtccaatg
    10561 ccagtttgac gatttcgcgg atgtaaatcg tgttttccgc aaccgtaccg cgcgacagct
    10621 ctctgaccgc aatcagttgg ttttggcggt tcagcagcag cgcgacgctg acttcgactt
    10681 tttcctgccc caaatgaaag cgtaaataat cggcaacggt atccggatcg gacaggacga
    10741 tgctctcctg caattcttcc gccaatatcc gccgcccgat ttccctgact acggcaaact
    10801 gtgtgaaact tgccgtcccc atccctttgt atgccgacag tttgccgacc tccgcgctca
    10861 tcagcctccc caaactgccg aactcctgca gcaaataacg cgccaaatcg accgcactca
    10921 ttccgcgcgt gccgacgcgc agcaggattg ccaaaagttc ggcatcactc aaagccgccg
    10981 ccccgcgttc caacagcttt tccctgggtc tttcgccttc cggccattgc ttgatgctca
    11041 tgttttccct tttttaaata gtctgagatg ccgtctgaaa caaatgttcc cacacgccaa
    11101 atatccgaaa tgccgtctga accggatttc cgcttcagac ggcatctgta tttcaaaaca
    11161 gtcttacgcc gtttcttcca actcgatgga cgccgccaga agcgacaggc gtgccattac
    11221 gccgtaaacg tagaggcggt tgtgttcgtt gtccacgtcg cagtcttttt cagagcgcgg
    11281 catacccagc gagtcccatt gttcgaatac gcgcttgcag gcttcgggag cttcttggga
    11341 gttgtcgccg ttgccggtgg ggatgctgtt ggacagcgga acaaacacca taccgccggc
    11401 gttgaggttt tcgtccgcac cgcgcccttc gtgtacgcgg aaaaagccgc cgatgacgaa
    11461 gcggtccatc atatagacga cgggttcgca caccgcgccg tttaaggttt cataagtata
    11521 aatcccttct tggacaatca cttcgctgac ttccaagcct tctttgactt tcgccatttt
    11581 attgcggttt ttacggttca atccgcgcac ttcgtcggcg gatttgacgc tcatcacgcc
    11641 catgccgtaa gtgccggcat cggctttgac gatgacgaaa ggtttgtcgg taatgcccga
    11701 ttcgtcgtat ttggcttgaa ttttcgccag cacacgttct accgcttccg ccaacgcgtc
    11761 ttcgccttca cgcccttgga agtccaaacc gccgattttt tcaaaataag ggttaatttg
    11821 ccattcgtcg atgccgatta acttggaaaa ttcggcggta acttggttgt acgcgccgaa
    11881 atgatttgtt ttgcggcgcg tcgtccaacc gccgtgcaac ggcggcaaaa cggtttggct
    11941 gatgcctttg aggatgtcgg gaatgccggc agacaaatcg ttgttcaaca aaaccacgca
    12001 aggcgaaaag ccgtctgcaa gatggacgcg gtcgcgggta cgcaataaag gttccaacag
    12061 gattttgtcg cccaatgcag tttcaaattc ggtcggttcg gttacttccg gattcaggct
    12121 gcccaagcgc acttcatacc ctgccgaacg caaaatctcg ccgagggcgt aaatgttttg
    12181 caggtaaaac gtattgcgcg tgtggttttc gggaataatc aatacggatt tcgccgtttc
    12241 gcaggcgcgt tgcaccgcat cttgcgcggc aaccgccgcc agcgggataa agttgggatt
    12301 cagattattg aagccgccgg ggaacaaatt catatcgata gacgaaattt tgtaaccggc
    12361 attgcgtata tcgaccgaac cgtaaaacgg cgggcggtgt acgctccact gcgtgcggaa
    12421 ccacgcctcg attttggcgt gattggacag gatttttgcc tcaaacgcct gaagttgcgc
    12481 cgaatgttcg ggcgacatca ccggtaattt catctttcgt ctgcctctgt gtgttttttg
    12541 acaagatgca gatgataatg cctttgaaag aatgacgaca agtattcttt cgtatcgaat
    12601 catgctttag atataactgt ttactgaaat ggacaatacg tctaattttt tcgttaaaat
    12661 tgtttgcaaa ataacaccgg ctcaaaaatt agacaaaatt tgttgcgcgg tataaagaat
    12721 acgtctaaaa tccgcccaat ccgcattacc atttatccaa aagaacaaca tcatgacagc
    12781 acaaaccctc tacgacaaac tttggaacag ccacgtcgtc cgcgaagaag aagacggcac
    12841 cgtcctgctc tacatcgacc gccatttggt gcacgaagtt accagccctc aggcatttga
    12901 aggcttgaaa atggcggggc gcaagctgtg gcgcatcgac agcgtcgtct ccaccgccga
    12961 ccacaacacc ccgaccggcg attgggacaa aggcatccaa gacccgattt ccaagctgca
    13021 agtcgatact ttggacaaaa acattaaaga gtttggcgca ctcgcctatt ttccgtttat
    13081 ggacaaaggt cagggcatcg tacacgttat gggccccgaa caaggcgcga ccctgcccgg
    13141 tatgaccgtc gtctgcggcg actcgcacac ttccacccac ggcgcattcg gcgcactggc
    13201 gcacggcatc ggcacttccg aagtcgagca cacgatggcg acccagtgca ttaccgcgaa
    13261 aaaatccaaa tccatgctga ttgccgttga cggcaaatta aaagcgggcg ttaccgccaa
    13321 agacgtggca ctttacatca tcgggcaaat cggcacggcg ggcggtacag gctacgccgt
    13381 cgagtttggc ggcgaagcca tccgcagcct ttctatggaa ggccgcatga ctttatgcaa
    13441 tatggcgatc gaagccggtg cgcgctcagg catggttgcc gtcgaccaaa ccaccatcga
    13501 ctatgtaaaa gataaaccct tcgcacccga aggcgaagcg tgggacaaag ccgtcgagta
    13561 ctggcgcacg ctggtgtctg acgaaggtgc ggtattcgac aaagaatacc gtttcaacgc
    13621 cgaagacatc gaaccgcaag tcacttgggg tacctcgcct gaaatggttt tagacatcag
    13681 cagcaaagtg ccgaatcctg ccgaagaaac cgatccggtc aaacgcagcg gtatggaacg
    13741 cgcccttgaa tacatgggct tgaaagccgg tacgccatta aacgaaatcc ccgtcgacat
    13801 cgtattcatc ggctcttgca ccaacagccg catcgaagac ttgcgcgaag ccgccgccat
    13861 cgccaaagac cgcaaaaaag ccgccaacgt acagcgcgtg ttaatcgtcc ccggctccgg
    13921 tttggttaaa gaacaagccg aaaaagaagg cttggacaaa attttcattg aagccggttt
    13981 tgaatggcgc gaaccgggct gttcaatgtg tctcgccatg aacgccgacc gcctcgcacc
    14041 ggggcaacgc tgcgcctcca cctccaaccg caattttgaa ggccgtcaag gcaacggcgg
    14101 acgtacccac ctcgtcagcc ccgctatggc agccgccgcc gccgttaccg gccgctttac
    14161 cgacatccgc atgatggcgt aagtattttc aggcggtctt taagaatttg acgcggccgt
    14221 ctgaaatata tcccgtaagg cagaaacacc tttaatattc aaaccaagga gtttaaccat
    14281 gaacaaactt ttcattaccg ccctgtccgc cctcgccttg tccgcctgcg ccggcacttg
    14341 ggagggcgcg aaacaagaca ccgcccgcaa ccttgacaaa acacaggccg ccgccgaacg
    14401 cgccgccgaa caaacaggca acgccgtcga aaaaggctgg gacaaaacca aagaagccgt
    14461 caaaaaaggc ggcaatgccg tcggacgcgg catttcccat ctcggcggaa aaatcgaaaa
    14521 cgccaccgaa taaccgattc gcgatgccgt ctgaaaccgc ttcagacggc atcgtcagca
    14581 caagacgcaa acccatgaaa gcctttacca aaattaccgc catcgtcgcc ccgctcgacc
    14641 gcagcaacgt cgataccgac gccatcatcc ccaaacaatt tttaaaatcc atcaaacgca
    14701 gcggcttcgg ccccaatgcc tttgacgaat ggcgttacct cgaccacggc gaaccaggca
    14761 tggacaacag caaacgcccg ttgaaccctg atttctcgct gaaccagccg cgttaccaag
    14821 gcgcgcaaat cctgttgacg cgtaaaaact tcggttgcgg ctcttcacgc gaacacgccc
    14881 cttgggcatt ggacgactac ggcttccgcg ccgtcatcgc ccccagcttc gccgacatct
    14941 tctttaacaa ctgctacaaa aacggccttt tacctatcgt gttgaccgaa gaacgggttg
    15001 accgactttt caaagaagtt gaagccaacg aaggctatca gctctccatc gaccttgccg
    15061 aacaaaccct gaccacacca agcggcgaaa cgttcacatt tgacattacc gaacaccgca
    15121 aacactgcct cttaaacggc ttggacgaaa tcggtctgac cctgcaacac gccgacgaaa
    15181 ttcacgcctt tgaagaaaaa cgccgccaaa gccagccttg gctgtttaac ggttaaaacc
    15241 tataccgtct gaaggccgtt cagacggcat tcctgataac atataattgt tgcggctaat
    15301 gtaaacagag gggcgataaa ttgctttcat cttttaagcg ggcttagcga cactctaaat
    15361 taatgcaata atgcaactct ccatttcaaa taaattttta gatttttaat atgcaacaaa
    15421 ttaaatttat tgacttattt tccgaaatga gtggcatcag gaaaggattc gaacaagcct
    15481 gtcggaaaca gtcggttgct tgcgaatgtg tttttacttc agaaatcaaa ccggcagctt
    15541 tggaagtatt gaagcaaaac taccctgatg aagtgccgta tggagatata acgaaaattg
    15601 aaacagggga tattcccgat tttgacatcc tgttggcagg cttcccttgt caggcttttt
    15661 ctttcgcctg aaaaagatta ggctttgaag atacacgggg aacgcttttc tttgatgtgg
    15721 caaggatttt aaaggcaaaa aaaccaaaag gttttatttt ggaaaatgtg gaaggattgg
    15781 tgacacacga tagaaaagat tcgacacaaa aaatagggcg caccctgacc gttattttgg
    15841 aaaccttgga agcattgggc tactatgttt cttggaaagt tttaaatgca aaagatttcg
    15901 gcattcccca aaacaggaag cgcatttatc tgacaggcag tttgaaatcc aaacccgact
    15961 tatctttcga aacaagccca agtccgaaat taaaaaatat tttggaatcg ggactgccta
    16021 ccgaaagcag ccctttcatc aaaaaattgc taaaaaaatt tcccccgtcc gaactgtacg
    16081 gaaaatcagt aaaagacaaa cggggaggga aaaacaatat tcacagttgg gatattgaat
    16141 taaaaggcgc agtaaccgaa gaagagaagc aattgttaaa tatccttcta aaagaacgga
    16201 ggaaaaaaaa tgggcttcag aaatcggcat agattggatg gatgggatgc ctttgacaaa
    16261 agcgcaaatt tcaactttct ataaacaccc cgatctccaa aatattttgg acagcctgac
    16321 agataaaggc tatttggttt tagagcatcc gaaacaaaaa attggcgggc aaagaatcaa
    16381 agatgaatcc ctgcccaaag gctacaatat tgtttcaggc aaaaaatctt ttgaaatcaa
    16441 taaaatatta gatcaaaacg atgttgcgcc aaccttggtt gcaatgaata tggagcacct
    16501 tttcgtcgtt gacaacggcg gtttgcgtac acttaccgga aaagaagggt tgcgcttatt
    16561 cggctatccg gacgattatt cgtttgatat tcccaaaaaa gacagatgcg atttattggg
    16621 taataccgtt gccgtccctg tgattaaggc ggtatctgaa agacttctgc atactttata
    16681 aaccgacagg gataaaatga taaaacttac tgcacaacaa atatttgata agttgctgga
    16741 tgaagaaaaa atcttatcgg ccaatggtca aattagattt ttcttaggcg atgtggatat
    16801 tatcgtcaaa caaaaagatg ttgtcggcaa catcattcag gaatggctcg gcggatgatt
    16861 gagaaaaagg gaaattgaat ttgatgtttc aaccaatacc caaatgcctc ccgatttctt
    16921 tttaaataaa aaagaccgca gcagagaatt gttggaagta aaggcgttca accgaaatgc
    16981 cggcccgggt tttgatattg cagattttaa aatgtattct gataaaatca ttcataagcc
    17041 ctatatgctg gatgtagact atttaatatt cggttacgat atggacgaca acggcaatgt
    17101 aaccatcaag gatttatggc ttaaaaaagt atggcaaatt acgagaagca tggatgggtg
    17161 ggcaattaat cttcaagtca aaaaaggcgt ggtgcataaa atccgcctgg gtgtttggta
    17221 cagcataaat aaaaagaata tgcctatgtt tgaatgcttg gaagattttg tttccgcaat
    17281 tgaagaaacc gtttatcaaa acccggcaac acggcataac gcatccttat ggaaaaagaa
    17341 atttgaggaa gcctataaaa aacactacaa ccgatcgatt tccatacctc gttggcacga
    17401 aattgcacac aaatacaaaa agaaataacc cttcaaccat caacccataa ggaaacttga
    17461 aatgaccaaa catatcgcca tcctccgagg tgacggcatc ggtcccgaaa tcgtcgccga
    17521 aaccgtccgc gtactcgaca aactcatcgc ccaaggtttg gatgccggct acgaatacgc
    17581 cccattgggc ggcgaagcct atgacgaata cggtcatcct tatcccgaat ttacgcaaaa
    17641 cctctgccgc aaagccgatg ccgtcctgct tggtgcagtc ggttctcctc aatacgacaa
    17701 cctcgaccgt ccgttgcgcc ctgagcgcgg cctgttggca atccgtaagg atttgaacct
    17761 gtttgccaat ttgcgtcctg ctgttttata tcccgaatta gccaacgcct ccacgctgaa
    17821 gcctgaaatc gttgctggtt tgaatatttt gattgtgcgc gaactcaccg gcgacatcta
    17881 tttcggcgaa ccgcgcggca tccgcgttct ggaaaacggc gaacgcgaag gctacaacac
    17941 catgaaatac agcgaaagcg aaatccgccg catcgcccac gtcgctttcc aatccgccca
    18001 aaaacgcagc aaaaaagtct gctccgtagg caaagccaac gttttggaaa ccaccgaact
    18061 gtggcgcgaa atctttgaag aaatcggcaa acaatatccc gatgtcgaac tttcccatat
    18121 gtatgtggac aacgccgcta tgcagctcgt gcgcgcgccc aaacagtttg acgtgattgc
    18181 caccggcaac atcttcggcg acatcctctc cgacgaagcc tccatgctga ccggctccat
    18241 cggtatgctg ccttccgctt ctttggacga aaacggcaaa ggcctgtacg aaccgtccca
    18301 cggctccgct cccgacatcg ccggacaaaa caaagccaac ccgctggcca ccatcctctc
    18361 gctcgcaatg ttgctgcgtt acagcctgaa cgacgaagcg cgcgcgcaac aagttgaaaa
    18421 cgccgtccaa aaagtgctgc aacaaggctt gcgtaccagc gacatttacg aagaaggcac
    18481 aaaactcgtt tcctgctccg aaatgggcga tgcggtactc gccgccttgt aaaaaaacaa
    18541 agcgcggatc acaccgcgct ttttcatctg cccttttccg ctcgccccgt tcgtcaaccg
    18601 cctgcaaagc caccgtttac cggcaggaaa tgccataaat ccccgcctat gttaggataa
    18661 acgcctttac tggctcacac aaaggattat catcatgaaa aaaatcatct tcgccgcact
    18721 cgcagcggca gccgttggca ctgcctccgc cgccacctac aaagtggacg aatatcacgc
    18781 caacgcccgt ttcgccatcg accatttcaa caccagcacc aacgtcggcg gtttttacgg
    18841 tctgaccggt tccgtcgagt tcgaccaagc aaaacgcgac ggtaaaatcg acatcaccat
    18901 ccccgttgcc aacctgcaaa gcggttcgca acactttacc gaccacctga aatcagccga
    18961 catcttcgat gccgcccaat atccggacat ccgctttgtt tccaccaaat tcaacttcaa
    19021 cggcaaaaaa ctggtttccg ttgacggcaa cctgaccatg cacggcaaaa ccgcccccgt
    19081 caaactcaaa gccgaaaaat tcaactgcta ccaaagcccg atggcgaaaa ccgaagtttg
    19141 cggcggcgac ttcagcacca ccatcgaccg caccaaatgg ggcgtggact acctcgttaa
    19201 cgttggtatg accaaaagcg tccgcatcga catccaaatc gaggcagcca aacaataaat
    19261 cttgatgcga atcagcacaa atgccgtctt catcaaaccg ccccgccaac ggtaagccga
    19321 tatggcggag cacaatccgc cgaaagatat ttcagcggca ggcagggctt gccggaaagt
    19381 ccggaaagta tagtggatta acaaaaacca gtacggcgtt
//
LOCUS       DAWHRS010000018        19328 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWHRS010000018 DAWHRS010000000
VERSION     DAWHRS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.018"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(142..2748)
                     /gene="acnD"
                     /locus_tag="WIB82_000462"
     CDS             complement(142..2748)
                     /gene="acnD"
                     /locus_tag="WIB82_000462"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ3841812.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGSVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGTLA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVIGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(2826..3614)
                     /locus_tag="WIB82_000463"
     CDS             complement(2826..3614)
                     /locus_tag="WIB82_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ3841813.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFVGGVAGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(3767..4921)
                     /gene="prpC"
                     /locus_tag="WIB82_000464"
     CDS             complement(3767..4921)
                     /gene="prpC"
                     /locus_tag="WIB82_000464"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ3841814.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5007..5882)
                     /gene="prpB"
                     /locus_tag="WIB82_000465"
     CDS             complement(5007..5882)
                     /gene="prpB"
                     /locus_tag="WIB82_000465"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ3841815.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6508..7818)
                     /locus_tag="WIB82_000466"
     CDS             complement(6508..7818)
                     /locus_tag="WIB82_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ3841816.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(7957..9135)
                     /gene="ftsZ"
                     /locus_tag="WIB82_000467"
     CDS             complement(7957..9135)
                     /gene="ftsZ"
                     /locus_tag="WIB82_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ3841817.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9254..10498)
                     /gene="ftsA"
                     /locus_tag="WIB82_000468"
     CDS             complement(9254..10498)
                     /gene="ftsA"
                     /locus_tag="WIB82_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ3841818.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10584..11312)
                     /locus_tag="WIB82_000469"
     CDS             complement(10584..11312)
                     /locus_tag="WIB82_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ3841819.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKTLGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11302..12216)
                     /locus_tag="WIB82_000470"
     CDS             complement(11302..12216)
                     /locus_tag="WIB82_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ3841820.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12329..13738)
                     /locus_tag="WIB82_000471"
     CDS             complement(12329..13738)
                     /locus_tag="WIB82_000471"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ3841821.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYLEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLALSKQI"
     gene            complement(13893..14960)
                     /gene="murG"
                     /locus_tag="WIB82_000472"
     CDS             complement(13893..14960)
                     /gene="murG"
                     /locus_tag="WIB82_000472"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ3841822.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGGLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(14964..16289)
                     /locus_tag="WIB82_000473"
     CDS             complement(14964..16289)
                     /locus_tag="WIB82_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ3841823.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16415..17752)
                     /locus_tag="WIB82_000474"
     CDS             complement(16415..17752)
                     /locus_tag="WIB82_000474"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ3841824.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRDDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIVTQDIPLQGLHN
                     AANVMAAVALCEAIGLSREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLGEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(17890..18108)
                     /locus_tag="WIB82_000475"
     CDS             complement(17890..18108)
                     /locus_tag="WIB82_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ3841825.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGHGVRQDRALAQEWLGKAC"
     gene            complement(18223..19305)
                     /locus_tag="WIB82_000476"
     CDS             complement(18223..19305)
                     /locus_tag="WIB82_000476"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ3841826.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4617 a         5367 c         4883 g         4461 t
ORIGIN      
        1 aaggtttcct caattccact ttctggattc ccgcctgcgc gggaatgacg atttacagta
       61 gatgcctgcc atatcgagaa ttacgtgaat gagcaaaatg ttgaacccga cccacgctac
      121 gtcttgcttt tcagacgacc tctaagccgc gttcccttcc aaaaaatcct gtgcaaaccg
      181 ttgcaacacg ccgccggctt catataccaa tacttcttct gcagtatcga ggcggcaggt
      241 aaccggaact tcgacggttt ctccgttttt acggtgaatc acgagggtca ggtcgcagcg
      301 cggtgtacgt tcgccgataa cgtcgtaggt ttccgtaccg tccagttgca gggtatggcg
      361 gttggtaccc ggtttgaact gcagcggcaa cacgcccatg ccgataaggt tggtgcggtg
      421 gatacgctcg aagccttcgg caacaatcgc ttctacgccg gcgaggcgta cgccttttgc
      481 agcccagtcg cggcttgagc cttgaccata gtccgcaccg gcaatgatga tgagcggctg
      541 tttgcggttc atataggttt cgatggcttc ccacatgcgc atggtttcgc cttcgggttc
      601 gacgcgggcg aacgagcctt ggcgcacgct gccgtcttcg tttttcacca tttcgttaaa
      661 cagtttcgga ttggcgaagg tagcgcgttg ggcggtcaag tggtcgccgc ggtgggttgc
      721 gtaagagttg aagtcttctt caggcaaacc cattttcgcc aaatactcgc ctgcggcact
      781 gacggccaaa atcgcattgg acggcgagag gtggtcggtg gtgatgttgt cgggcaaaat
      841 cgccagcgga cgcatacctc ttaatgtgcg ttcccctgcc agcgtgcctt cccagtaagg
      901 cggacggcgg atgtaggtgg acatcggacg ccaatcgtac agcggactgg gtgctttttg
      961 cgctgtgccg gtgtcgaaca tcggtacata cacatcgcgg aactgctgcg gtttcacata
     1021 ttcggcaacg acggcatcga tttcttcatc ggcaggccaa atgtctttca ggcggatttc
     1081 cttgccgtct gcaacgccga gtacgtcgtt ttcaatatcg aaacggatac tgcctgccag
     1141 cgcgtaggca acgaccaacg gaggcgaagc gaggaaagcc tgtttcgcat acgggtggat
     1201 acggccgtcg aagttgcggt tgcctgataa tacggcggtg gcgtacaaat cgcggtcgat
     1261 gatttctttc tggattttcg gatccagcgc gccactcatg ccgttgcagg tggtgcaggc
     1321 gaaggcgacg ataccgaagc cgagtttttc catttcgggc aacaggcccg cttctttcaa
     1381 atagatttcg gctacttttg aacccggggc aaacgaagat ttcacccaag gtttgcgttt
     1441 caagccgaga cggttggcat tgcgtgccaa gagcgcggcg gcaacaacgt tgcgcgggtt
     1501 ggaagtgttg gtgcaactgg taatcgcggc gatgatgacc gagccgtcgg gcatttggcc
     1561 gtccgaaggc tcttcgtaag gcttcgccag ccctttcgcc gccaaatcgg cggtcgcaaa
     1621 acgggcatgc gggttacttg ggcctgccat attgcgcgtt acgctgctca aatcaaattt
     1681 caaaacgcga ggataaacgg cggttttcaa ggcatctgcc cacaagcctg cggttttggc
     1741 gtaggtttcc accaatttca cctgcgcgtc gtcgcgtccg gtcagtttca aataatcaat
     1801 ggtttgctca tcaatagcga acatcgcggc agtcgcgccg aactccggcg tcatgttgga
     1861 aatggtcgcg cggtcgccga tagacaggct tctcgcgccc tcgccgaaga attcgacaaa
     1921 cgccccgacc acgcgttctt tgcgcagaaa ctcggtcagt gccaacacaa tatccgtcgc
     1981 cgtaatgccc gcctgccgtt tgccgttcag ctcaacgccg acaatatcgg gcaggcgcat
     2041 catggacgcg cgtcccagca ttacggtttc cgcttccaat ccgcccacgc ccacggaaat
     2101 cacgcccaat gaatcgacgt gcggcgtatg tgagtcagta ccgacgcagg tatcggggaa
     2161 agccacgccg tttttgactt ggacgacggg cgacattttt tctagattga tttggtgcat
     2221 gatgccgttg cccgccggaa tcacgtccac attttcaaac gcggtttttg tccagttgat
     2281 gaagtggaaa cggtcttcgt tacggcggtc ttcgatttcg cggtttttgc ggaaggcatc
     2341 aggatcgtaa ccgccgcact ccaccgccag agagtggtcg acgatgagct gggtttgcac
     2401 caccggattc actttggcag gatcgccgcc tttttcggca atcgcatcgc gcaggcctgc
     2461 caaatccacc aacgcggtct gccccagaat atcgtggcac accacccgcg ccggatacca
     2521 cggaaagtcg atttcctgct tcccttctat caactgcccc agccagcttt gcagcgtcgg
     2581 caaatcgact ttgtccgcgc ggttgaccaa attctccgcc aaaatgcggc tcgtgtaagg
     2641 cagcttgtcg taagagccgg gcttgatgtc ctcacacgcc gcacgcgcgt cgtagtattc
     2701 caaatccgta ccgggcagcg gtttgcggta acgttggttg gcagccatgt cggttctcct
     2761 gtggatctgt ttttcttgtg gtttgaggtt tcagccgacg ttttgaaggg gtcgtctgaa
     2821 agggtttaaa acatcgaaac aatcatctga tacagcggat ttctctcgtc tatcaacaat
     2881 ttcacagcca tcgaaatgct gatgacaatc agcagcggct taatcagctt cgaaccgaag
     2941 cggacggcaa atctcgcacc taaattcgca ccgacaaacg caccgaccgc catcgttgcc
     3001 gcaatcggga aaataatcga accgtgcagc aggaataccg atagcgaacc aagattgcag
     3061 gcaacgttcg ccaatttggt gtaagacatc gcgttcaaca gcttgcagcc gagcaaaaca
     3121 ataaaggcaa tcagaaaaaa cgagccgaca cccggtccga acacaccgtc gtaaaaaccc
     3181 aaaagcggtg cgaccgtcag cccgaacaga aaaaaagaca ttctggcttt gccttcctta
     3241 ctgccgtcga gcttgggcga aaacacaaaa tacagtgcga caaatatcaa caaaaccggc
     3301 acgaccgcca gcagaatatc tttggaaacc aagctgaccg ataatgcacc ggccacgccg
     3361 cctacaaacg atgctgcggc aatcgggaga cctttcttcc aatcaatcaa acctttgcgt
     3421 gcaaaagaaa ccgtagctga aaacgtagca gcggctgctt gcagcttgtt ggtggcaatt
     3481 gccgacacgg gaggaatacc tgccaacaag agtgcgggca gcgtaatcaa accacccccg
     3541 cccgcaatcg catcgataaa tccggcaatc atcgcaacca aacccaaagc gagtattata
     3601 tataaatctt ccatgtttct tatcctgtta cttgcgccaa tacaggatta tcttctccta
     3661 ttagattaaa cttatttcag acaacctttc caataaggca aggtcgtctg aaatccttag
     3721 ctttgcatac cgaaattaaa gaacaaactg ctattgcatt cttcaattat cgctcttcaa
     3781 tctccacaaa cgccaaatct tcagggcctg tgtagtttgc gctcggacgg atgattttgc
     3841 cgtctttgcg ttgctcaaga acgtgtgcgc tccaaccggt tgtacgggaa attacgaaca
     3901 gcggtgtgaa catagcggtc ggtacgccca atttttggta ggaaacggca gagaaccagt
     3961 ccagattcgg gaacattttt ttctcttccc acatcacgct ttccaaacgt tcggcaatgt
     4021 caaagaggcg catatcgccg gtttctttgc tcaaaccgcg tgccacttct ttaatgacaa
     4081 cgttgcgagg gtcggaaatg gtgtacaccg gatgaccgaa accgatcacg atttctttgc
     4141 ggccgatgcg ttcgcggatg tcggcttcag cttcgtcggc attgcggtag cgtttttgaa
     4201 tatcgtaagc cacttcgttc gcgccgccgt gtttcggacc tttcaacgcg ccgattgctc
     4261 cggtaatgct ggagtacata tcagagcctg taccggcgat cacgcgggcg gtaaaggtag
     4321 aagcgttgaa ctcgtgttcg gcatacagaa tcagtgaaac gtgcatggct ttgatgtgtg
     4381 attcgcttgg gcgtttgccg tgcaacagtt gcaggaaatg accgccgatg gtctcttcgt
     4441 cgctttcaac ctcaatgcgt ttgccgttgt gcgaatattg ataccagtac aagaggatgc
     4501 tgccgaggct ggcgatcagt ttgtcggcga tgtcgcgcgc ttcactttcc ggatggcttt
     4561 cacgttcagg atgaacgcag cccagcatgg atacgccggt acgcattacg tccatcggat
     4621 gggtatgtgc aggcaggctt tccaaaactt taatcacacg gataggcagg ccgcgcatgg
     4681 atttgagctt ggttttataa gcggccagct cgaatttgtt gggcagatgg ccgtgaatca
     4741 gcaggtgggc gacttcttca aactcgcatt tttgtgccaa atccagaatg tcgtaaccgc
     4801 gatagctcaa atcgttgccg gtacggccaa cggtacacaa agcggtatta ccggccgcaa
     4861 cgccagaaag cgcaacggat tttttaggtt tgagggtcgg ggtttgagta gtttcagtca
     4921 tggtatttct cctttgtgtt tttatgggtt tcgggttttc agacgaccga tgcggatttg
     4981 ttgaaaggca gtctgaaagc ggtaaatcat ttttgaaaca atttatccag tttttgctcg
     5041 aaggcatgat agttcagatg ctcgtacagc tcggcacggg tttgcatact gtccaccacc
     5101 gccgcctgag tgccatcgcg cataatcgct tcgtaaacat tcagagcggc tttgcttgct
     5161 gcacggaacg atgacagcgg atacagcacc agcgacacgc cgttttcagc cagctcgctt
     5221 tgggtataaa gcggagtgga accaaactcg gtaatgttcg ccaacacggg cactttcacc
     5281 gcatctgcaa attggcggta catgttcaaa tcggtcatgg cttcagggaa aatcatgtcc
     5341 gcaccggctt cgacacaagc ttgggcgcgt tcgatagcgg catccaaacc ttctaccgcc
     5401 agcgcatcgg tacgcgccat aatcacgaag ttctcatcaa cgcgcgcatc tacggcagct
     5461 ttgatacggt cgaccatttc atctttagat acaatggctt tgttcggacg gtggccgcag
     5521 cgtttttgcg ctacctgatc ttcgatgtga accgctgcaa caccggcgcg ttcaaagttg
     5581 cgaatggtac gggcaatatt gaatgcaccg ccccaaccca catcgatgtc caccagcaga
     5641 ggcgtatcca cgttgtccgt aatgcgtcgt gcgtcgatca gcacatcttc cattgtggta
     5701 atgcccaaat cagggatacc gcaagaacag gctgccacgc cgccgccgga cagatagatg
     5761 gctttgaaac cgctttgggt ggccaatcgt gcaaaataag cattgacgca accggcgacg
     5821 gcaagcggat tcgattcttt cacggcttgg cggaaacgtg ctccggcaga gtgttgactc
     5881 atcatatttc tcctttatag actttttttc agtatggaca ggcttccatc acattcggac
     5941 ggcaaaacac agccatccga cgcgtcgggc atcccaatcc aatcattaaa aatatatggg
     6001 aaaaattatc ttattgatat ttaaaacgaa tcaaagaaaa acagcagacc gttcggaatt
     6061 atgcggcaaa accgcagaca agaagaaaac aaggggatta ttcagaaaag gggaaaacat
     6121 ctgaattggt ttcatagtaa tgttcctttg tagtgttatg tagttttatt tttcgataat
     6181 ctaaaacaca agactgaaaa tgtcaaacat ttttaaggag actcattaca taattttaat
     6241 attttatttt aattccatga ttaaactaga ttaattttga attatttgtt tcaataaaat
     6301 tgtcaggaaa tcaagaaaaa tttttgataa tcaaaatcct gaaaacttat aaaatatttt
     6361 atacataaaa acaatgtatt atttttaagc aatccgatgt attttattat gtatttgatt
     6421 tttatataat ttaataaaaa ttaaatatat cggcaaacaa aatgccgtct gaaaaccttt
     6481 cagacggcat ttttaatatc gaatcaatca gcccaaatac cagaatttca gtgcccacaa
     6541 tacggcaaca atccatacca taggcggaac gtctttggtg cggcggcata aaagtttaac
     6601 cacggcataa ctgatgaagc cgaaagcgat gccgtctgca atcgaataag taaacggcat
     6661 gaaaacaatg gtcaggaacg caggtgcggc ttccgtcata tcgtcccaat caatatccct
     6721 cgcactgcgg agcatctgcg tgccgacata aagcagggcg ggcgcggtgg caaaagcggg
     6781 aacacttttc gccaaaggtg aaaacatcag gcaggcgagc atcaatacgc cgacggtaac
     6841 cgccgtcagg ccggtccgtc cgcctgccga tacgcccgcc gcgctttcca cataaggcgt
     6901 ggtggaagaa gtacccaaag ccgcacctgc cacaatggcg gtagagtctg caagcagtgc
     6961 gcgtttcagg cggggcagct taccgtccac cagcagcccg gcacggtggg atatgccgac
     7021 cagcgttccg gtactgtcaa atagatcgac caagaagaag acgaaaatca cactgaccat
     7081 gctgacggta aacaggcctt caaaatccat ctgcataaaa gtcggcgcaa tgctcggtac
     7141 ttcgccgatg atgccgtgaa attcattcaa acccatcagg ctggcaatga cggtaatggt
     7201 caagatggtg atgatgattg cgccttgaac gcggaaatgt cccaatacga ccaccatagc
     7261 aaaaccgaac aatgccaaca acgcggacgg ctgatgaata tcgcccaaac cgaccaaggt
     7321 tgccggattg gcaacgataa tgcctgcgcc tttcagggaa atcagtgcca aaaacaaacc
     7381 gataccggca gcaatcgaca ttttcaaacc cataggcagt gcgttgacca gcatttccct
     7441 gactttaaaa aagctgaaca ggataaaaat cagaccggag atgaacaccg cacccaacgc
     7501 aacctgccaa ggcacgccca tacccttaac gacggcaaag gtgaaatagg cattcagccc
     7561 catccccggt gcgagtgcaa tcggatagtt gccgacaaaa cccataacaa aacagccgat
     7621 ggcagacgcg atacaggtag cgacgaatac cgcccccata tccatgccgg tctcgcccaa
     7681 aatcagaggg ttgacgataa cgatgtagca catcgtcaaa aaagttgtca aacccgccat
     7741 caactcggta cgcaccgtcg taccgtttgc cttcagctta aaaatcccgt ccaacagtgt
     7801 ttgttttgaa gtgtccatat ctgaaagtct gtatcaagtg gaaaaatctg aatccgccga
     7861 ccgcaaaccg tacgaaattg caacatcttg aaaaacatac ctcccaaacc ggacaaaccg
     7921 attcgggagg cagcaggttt acgggcaaac agcacattat ttgtctgaat tgtgttgacg
     7981 acgcaaaatc gcagggattt caaagtcgtc aagtacggac tgattgtcga aatccgcagc
     8041 ggtaaggttc atcgtgcgga taccgcgatt ggtgcggatc ataccttcga cattgtggct
     8101 ttgctcctgt ttggacggag caaccgcttc tacctccctt gccggaacaa aatcgaccgc
     8161 gcctttttct ttcagaccgg tagcgataat ggtaatccgg atggcatctt cgctcatggt
     8221 ctcgtcttca gccgcaccga atttgcattc caaatcggga tgcgcgcttt ggttgacgat
     8281 tttcatgact tcggacaact cggacatttt caagcaaccc ggagcagtcg taatattgac
     8341 cagcacaccg cgcgctccgt ccaaggttac atcgtccagc agcggactgg aaatggcctg
     8401 gtcggtcgcc atacgcgcac ggtcgatacc ttgggcataa cccgaaccca tcatagcgat
     8461 accgcggttg ctcatcacgg ttttcacgtc ggcaaagtcg aggttgatga tttcgctcgg
     8521 gcaagttacc acttcggaaa tgcctgcgac cgcatcgcgc aatacattgt cggcggcacg
     8581 gaaggcttcg cgcatcgtta cgtcttcacc caatgcagtc atcagtttgt cgttcgggat
     8641 gataatcagc gaatcgacgt gttctttcaa ctgttccaac cctgcctgtg cgacatggac
     8701 gcgcttacct tcatatgcga acggtcgggt aaccacggca acggtcagaa tgcccaaaga
     8761 cttggcaatc tcagcaacaa ccggcgcgga accggtaccg gtaccgccgc ccataccggt
     8821 cgtgataaac agcatattcg caccgcgaat ggcttcttca atggcttccc ggtcttcctg
     8881 ggctgccgca cggccgatat cgggattcgc gcccgcgccc aaaccgcgtg tcagattcgt
     8941 acccaactgg attctcttcg ccgcatggtt ttttgccaga gactgcgcat ccgtattggc
     9001 actgataaac tccacaccgc gcacattgtt ggcaaccatg ttattgattg cattgcaacc
     9061 gccgccgccc aagccgatta ctttaatcac cgcagggctg actgccgatt ctgccacgtc
     9121 gtaaacaaat tccattcaaa aactcctgct cgccccattc agaggacggt ttaaataaat
     9181 tattattcat tatataagaa gtatcttgct gctggcaaaa tacttctcac ctgtcaaacg
     9241 gcaatccacc tgttcagaag ctgttttcaa tccaccgttt caatcttgcc aacaaaccgc
     9301 cgcccccttc cctctcttgc actgcaccgt tttccggctg cggcaagttt ccttccagct
     9361 tgcatgctgc atgaagcagc ccgatagcgg tagaaaaacg cggtgtgcgg acgcggtcgg
     9421 acaaaccgcc catttcttgg ggtgcaccgg tgcgtacagg caaatcgaag attttttcgg
     9481 caaattccac aatcccggtc atcatggaca caccgccggt cagaacgata cccgcattca
     9541 gcacttcttt ggggaaaccc gatttttgca gctcgcccag cactacgcca aaaatctcct
     9601 gaatccgtgc actgatgatt gctgccagaa ccttactgga aacctgacgc gatgtccggt
     9661 cacccacgcc cggaacttca atcatctcac ccaagccttc cgtatcgcat gatgccacgc
     9721 cataatggat tttaatgtac tcggcggcat cgagaggtgt tctcaacgat ttggacaaat
     9781 ctttggtaat cagattacca ccggccggaa tgacggacgt atggcggatg gcaccgttca
     9841 tataaacggc aatatcggtc gttccgccac caatgtcgat gacgcatacg ccgaggtctt
     9901 tttcatcttc agtcagcacc gcctgcccgc ttgccaacgg ctgaagcatg atctgatcgc
     9961 ttttcaaacc gcaccgctcg atacattttt ggacattctg cactgccgta cttgcaccgg
    10021 taatgatgtg cacccgcgta tccagacgca caccgctcat accgatgggc tccctcacgc
    10081 caagttgggt gtcaataatg tagtcttgaa ccacggcatc gagaattttt tgatcgggcg
    10141 ggatattgat tgcctttgcc gtttcaatgg cgcgatcgat gtctgcctgc gtgacttccc
    10201 catctttaat tttaaccaca ccttgcgaat tgagactgcg gatgtggttg cctgcgatac
    10261 ctgtggtaac gtgagtaatt ttggtatccg ccatcagctc ggcatcattg accgcctgcc
    10321 tgatggcttg gacggtggca tcgatattgg ttaccatgcc cgcgcgcaag ccccgtgaag
    10381 gagcctgccc caaaccgacg atgttgattt tgtcgtcatc ttgaacttcc ccgatcagtg
    10441 cgaggacttt agacgtaccg atatccagta cgctgatgta tctttgctgc tgttccattg
    10501 ttcgtctgct cttaaaactg attgaaattt gcgtcgcacc gtttcagacg gcacggccgt
    10561 aatctgtccg atacctgttc ccactattct tcggattctt tttcgggtaa accgtcggaa
    10621 gcatagcgga ctgaaaatcc gtccttatac ctcatatcca cataggataa ccgattttta
    10681 tttttacgca acagatgctg ccacgcttcg gtaaaaagcc ggaggcgttt catctcgttt
    10741 tcccgtccga gcctgacggt gatgccgttg tccaaaacga caatccacgc cgaacgtgcc
    10801 gtataggtca tctctttgat gcccaaaccc tgttttgcca aaacagtcga aaattcgtca
    10861 taacggcgga gcatttcggc agacgttcct tccgcgcctc tgaataccgg cattccgggt
    10921 ctgtccaagc gggcttcaaa aacattgcct tcgccgtcca ccaaggcatg gtcgccccaa
    10981 cgcgcgaccg gcttgcgctc ggtcaggacg acctcaaccg tgtcgggaaa acggcggcgc
    11041 accatgaccg acgcaatcca cggataccgg cggtaggcct cctgtgcgcc attgatgtcc
    11101 gtcctcaaaa tattcccatg gatgtattct ttcgccaaac tgcccaatgt cttcttatcg
    11161 gaataaacca ggttgccctt cagcgacacc tgcttgacgg gcagatgatt cgaattgtaa
    11221 aaccaaacca gcccggacgc agcaagcagc atcgccatca tgacaagcag ccagcgcgtc
    11281 agccgttcca tcgcttcggc attatcccac atgtgcggtc ttcaaaattt caatacataa
    11341 atcggcaaaa cccacgcccg taacggcagc ggatttcggt actaaactat ggctcgtcat
    11401 accgggcagg gtgttgattt ccaacagata gagtttgccg tcggtatctt tgaggaaatc
    11461 gacgcgcacg cagccttccg caccgattgc ctgcgcgccg cgaaccgcca gttcgcgcat
    11521 caggctttct tcggcttcgg tcaaatcttc cgaaggacat tgataaatgg tgtcgtcgcg
    11581 gttgtacttg gcttcgtagt cgtaaaactc ggttgcggga atgatgtgta tgccgggcag
    11641 ccctttgccg ttcaggacgg ggcaggaata ttcgccgccg ccgataaaac gttcggcaat
    11701 gatttcgccc tgaaggtgtt tcaattcttc gtaaacgctt ttcagacggc cttttccttt
    11761 gacttttacc acgcctacgc tgctgccttc ggccgccggt ttcacaaaca tcggcaggcc
    11821 caatttttct tcgacggcat cgaaatcagt gtcgtcgtgc aggacggcga actcgggaac
    11881 gggcaatccc aatgcctgcc aaatcagttt gcagcggtat ttgtccatgc cgatggcgga
    11941 tgcggcgaca ccgctgccgg tatagggaat gcccaacagt tccaatgcac cctgaaccgc
    12001 cccgtcttcg ccgtaagtac cgtgaaggat gttgaatgcc gtctgaaaac cttgtgcctt
    12061 caattcagac aatggggttt ctttaggatc gaaggcgtat gcgtctatgc ctttgctttt
    12121 taaagcattc aaaatggcgg tgccgctgtc cagcgagatt tctcgttcgc tggaaaaacc
    12181 gcccatcaat acggccactt tgccaaaatt ctgcattgtt tttgttcttt cctgattgct
    12241 ttatgcttgt tgccagaggt cgtctgaaac ctgatttgcg gtttcagacg atctttatat
    12301 gatgttccgt ctgtcaggcg ggtgtgcctc aaatctgttt cgacaatgcc agcagcgcgg
    12361 cggggacgcg gttgatgctt cccgcgccca tattcaacac gatgtcgccg tcctgcaaaa
    12421 cgttcaacag catttcgggc agatcggcaa cgttttcgca gtaaatcggc tcgagtttgc
    12481 ccaacacgcg gatggcgcgg gcaagagcgc gggaatcggc ggcggcaatc ggctcttcac
    12541 cggcggcata aacttcggtc agcaccagcg cgtcaacggt attgaggact ttggtaaagt
    12601 cttcaaacaa atcgcgcgtg cgggtatagc ggtgcggctg gaaggcgagt accaaacgtt
    12661 tttccagata cgcgccgcgt gcggcggcaa gggtcgccgc catttcgacg gggtggtgtc
    12721 cgtagtcgtc caccaagagc gcggtcccgc cgtttggcaa cttgatgtcg ccgtattttt
    12781 ggaagcggcg gccgacgcct tcaaagccga gcaagccttt ttggatcgct tcaaccgatg
    12841 cgccgacttc cagcgccacg ccgatggctg ccaatgcgtt cagcacgttg tgtctgccgg
    12901 gcatattcag cacgacttca aacgacccct gctcatgtcc tttcatttga acatggacgg
    12961 tgaatttcat ttgcgcgccg acgttttcga tgtcggtggc gtagatgtcg gcggtatcgt
    13021 ccaaaccgta agtagcataa ggtttgctca ctttgggcaa aatcgcgcgg acgtgttcgc
    13081 tgtcaataca caaaaaggct ttgccgtaga agggcatacg gtggatgaaa tcgataaacg
    13141 cctgatgcag tttttcgacg ctgtgcccgt aggtatccat atggtcttcg tcgatattgg
    13201 taacgacgga cataatcggt gtcaggtgca gaaaggatgc atccgactcg tcggcttcgg
    13261 caacgatgta ttcgcctttg cccaagcggg cgttggtgcc tgcggcgttg agtttgccgc
    13321 cgataacgaa agtcgggtca agtcctgccg cgccgaggat ggaggcggtc aggctggtgg
    13381 tcgtggtttt gccgtgcgtg ccggcaatgg cgatgccgtc acggaagcgc atcaactccg
    13441 ccaacatcag ggcgcgcgga ataacgggaa tttgctgctc caacgcagcg acaacttcgg
    13501 gattttcttt tttgacggcg gtagaggtaa cgacgacatc cgcaccgtta acgtgttcgg
    13561 cggtatggcc gggataaact tgaatgccca ggctgcccaa atgctcggta gcggcatttc
    13621 gcgcctgatc cgaaccggaa actttaaagc ccaaattgtg caagacttcg gcgatgccgc
    13681 tcatgccgac gccgccgata ccgacaaaat ggatgttggt aactcgattt ttcatcataa
    13741 tgttgcgttc cggtggattt tcgatgcgta aaggcgttat tttaaagggc tgaccgtttg
    13801 cgcgccatag ttttctgaca aatatatagc ggattgaaat aaaaaacatc catgccgtct
    13861 gaatggcttt tcagacggca tggttcggca gtttacgccg cacacgcaat cgcggcttcc
    13921 gccacgtcgt ccgcactgtg cggcagtgcc aacgtacggg cgttttctgc ccatttgagg
    13981 catttttcgc ggtttaagcc gccgagaatc tcggcgagtt tttccgccgt caactgggtt
    14041 tgcggcaaca gcaatcccgc ctccgcctgc accataaaac gcgcgttggc ggtttggtga
    14101 tcgtcaaccg cgtgaggata cggcactaac aacgcaccca atcccgccgc cgtcaactcg
    14161 gcaatcgtca gcgcgccggc acggcaaatc accaaatcgg catcgcggta ggcggacacc
    14221 atgtcggtaa taaattccac gcattcggct ttcacgccca gcgcgtcgta atccgcctgc
    14281 aagctgccca gcttgccccg tcccgattgg tggtacatct gcggacgcgc attgtcgggc
    14341 agcaaagcca atgcctgcgg tacggttttg ttcaaaacgt ccgcgcccaa actgccgccg
    14401 accaccaaaa ttttcagacg gccttcacgc ccttggaagc gttcggcagg cacgggcagg
    14461 ttgctaatat cggcgcggac ggggttgccg accaagccgc cttcgtggct gaacgctttc
    14521 ggaaaagcgt acaacacccg cttcgcccag cgcgacaggt ggcggttgga caaacctgcc
    14581 acggcgtttt gctcgtgaat cacaatcggc acgcctaata gcttcgccgc caaaccgccg
    14641 gggaaggtaa cgaagccgcc gaagccgatg acgcactcga cacggtgttt gcggataatc
    14701 cgctgcgctt cgcggacggt ttgatacaaa gtaaccggca gcatcagttt gcgtttgatg
    14761 ccgttgccgc gcacgccttt aatcgccagc gtttccaagc gtatgccgta ttgcggcacg
    14821 atacgctctt ccatcgaatc cttgctgccc agccaaatca catgatggcc gcgcgcgcgc
    14881 aatgaatccg ccaccgccag cgcggggaaa atatgtccgc ccgttccgcc cgccatcagc
    14941 ataaaggttt taccgcccat gatttactcc acccgataac cgcgcatttt ccggcggttt
    15001 tcataatcta tacgcaacag cagcatcatg ctgatcagca tgaaaaagac tgacgaaccg
    15061 ccataggaca tcaacggcag cgtcagacct ttggtcggca aagcaccgat gttcacaccg
    15121 atattgaaga aactttggat accgatccaa atgccgatac ccgaagcgat ataggcgttg
    15181 aaagtcaaac ccaaatcgcg cgactgcttg ccgatggaaa acgcccgcac caccagccag
    15241 ccgtaacaga atatcagcac gcacataccg aagaaaccga attcttcggc gatgatggca
    15301 aaaataaaat cggtatgcgc ttccggcaga aagccgcgtt tgctcaaact cgcacccaaa
    15361 cccataccga accactctcc gcgcccgatt gccatcagag agtgggtaag ctggtagccg
    15421 gcaccctgcg ggtctttcca cgggtccaaa aatgccacta cccgctgcac acggtaggga
    15481 gcggcggtaa tcatcagcac catcccgccc aagacgctgc ctaccaggac gaaaaaatat
    15541 ttccacggca atcctgccaa aaacagcatt ccaacggcaa tgacggtaat gacgacaaac
    15601 gaaccgaaat ccggctgtac cattatcagc accaaaccga acgccaccag cataatcggc
    15661 aggatgatcg cccggaaacg gccgtacatt tctaatgttt cacgacgtgc ctgcggattg
    15721 gtggcggaca tgatcagatt ggccgtcccc cgccaaatcg actgccaacc caaactttcc
    15781 atgctgcgca acacttcttc acggcgcgtg aacaggcttg ccaaataaag gatgaccgcc
    15841 agcttgaaca gctcggtcgg ctggaaattc aacggaccca aaggtatcca acgggtcgcg
    15901 ccattgattt cgcgcccggc aatcaatacg actaccagca acaggccgga tagggcaaaa
    15961 atccacggca caagccgccg ccatgtcctc atcctgcaaa gaaaccataa caaaccgctc
    16021 gctatcaagc cggcaacgac gaaccccgcc tgtctggtca aatagaaaaa ctgatcgccg
    16081 ccttcttttg atgccaaata cacagaagcc gaataaatca tcagcaggct gaacgccgtc
    16141 atcagcacca ccatccacaa aagcggcgcg tcgaatttcc tgccgtcgcg cacaatcggc
    16201 ctgtcgagca gcagagtgtg gacaccgtcg cccactttta ccaatacttc cgaaatcttc
    16261 aaaaaaacca cctgccagtc tgtttgcacc tgccgcaaag ggcaaaaatt tcagacggca
    16321 gacaatgccg tccgaacata cgatacatcc caaatcggta ttctaaatct ttacttgccg
    16381 cccaacaatg acggcgtttg catttcagac ggcatcacaa agccttaaac gcttcgataa
    16441 acacttccga acggtgcgcg tagcctttga acatatcaaa gctcgcgcag gcggggctga
    16501 gcaacacaat atcgcctgct tcggcttggg catatgccgt ctgaacggct tctcccaaag
    16561 tggcgcagtc ggtcatattc aagccgcagc cgtccaaatc gcggcggatt tgcggcgcat
    16621 cgacaccaat caagaacacg ccttttgcct tgcctaccag tgcatcgcgc aggggcgtga
    16681 agtcctgccc tttacccatg ccgcccaaaa tcacgaagag cggattttgc aaaccggcaa
    16741 tcgcggcggc agtcgcgccg acattcgtgc ctttgctgtc gtcgataaac accacgccgt
    16801 ttttctcgcc gattttttcc acgcggtgcg gcaggccttg gaaggttttg acgtgttcga
    16861 gcaatgcttc gcgcgacaaa ccgatggcct cacacaaagc cacggcagcc atgacgttag
    16921 cggcgttgtg cagaccttgc aacggaatgt cttgcgtgac aatcaaatct tcattgcctt
    16981 gtttcaggcg gcctgtctcg cgttccaacc agaaatcagc ttcgtgttcc aacgaaaacc
    17041 attttacctc gcgcccggca cgcttcatcg cgcggcagaa cgcatcgtcc gcattcaaaa
    17101 cctgcacgcc gtcgccacgg aaaatcttgg ctttggtatg cgcatagtcg agcaagtcgt
    17161 cgtagcggtc gagatggtct tcggaaatgt tcagcaccgt cgccgcagtc ggacgcaggc
    17221 tttcggtgtt ttccagttgg aagctggaaa gctccaacac ccacacgtcc gcctttttgc
    17281 cttcgcgctg ccattccgcc tccaaaaccg gcgtgccgat attgcccgcg ataacggtat
    17341 ccagcccgca cttgatacag agatagccga ccaggctcgt taccgtggtt ttgccgttgc
    17401 tgccggtaat cgcaattacc ttgtcgtccc ggcggttcac aatgtccgcc agcaattcga
    17461 tgtcgcccaa cacgcgtccg ccgttttgct tgaacgcctc aatatccggc tgccgctcgc
    17521 tgatgccggg actgagagcc agaatatcga aaccgttgtc cagcgcatct ttcagacggc
    17581 ccgtgtaaaa caccaacccg tcaaacatct taccgatttg cgacacgcgc tccggtttca
    17641 gctccgcatc atacgcggca acctccgcgc cgtttttgcg cagataggca atcatggaaa
    17701 tacccgtacc gccgagtccg gcgacgagga tttttttgtt ttgaaaagtc attttggttt
    17761 gtcctaaaac aaatcatatt gagcaggaga tgtccgcccc tgcccaagcc gctttcagac
    17821 ggcatcgcga gctgttcaat aacccgcctt caggcgttgg tcattgtcgc agccgtcttg
    17881 gtttccgttt taacaagcct tgccaagcca ttcttgtgca agggcgcggt cttggcgcac
    17941 gccgtgccct ttgtaataca ttgcgcccaa attgtattgg gctgctgcat ttccctgtgc
    18001 tgccgcctgc aagttttccc gaaaatccga tacgtcatcc gcccacaccg cttggttcaa
    18061 gcccaaggca atcagggcgg cggcaagcca tttgactgtc tgtttcatgg ttttacttct
    18121 gttttagtat aagccgccgt taacgataag gctgggcgga ttgtcgccgc aggtttattg
    18181 cgcgttcaaa tgccgtctga aagatgttca gacggcatag gttcagcgga ttttgagggt
    18241 actcaaaccg atcaacacca agacgatagt gataatccaa aagcggacga cgacttgggt
    18301 ttctttccag cctttttgtt cgtaatggtg gtggatgggc gccatcagga agatgcgttt
    18361 tttggttttc ttgtaccagc cgacttgaag cataacggat acggcttcta cgacaaataa
    18421 tccgcccata atgacgagga caaactcttg gcggacgatg acggcgacgg taccgagcgc
    18481 ggcacccaat gccaatgcac cgacatcgcc cataaagact tgcgcgggat aggcgttaaa
    18541 ccacaagaaa ccgaggcacg cgccgcacat ggcggtacag aaaatcacca cttcgtttgc
    18601 gccggcaacg taaggtaatt gcaggtattg ggcaaattgt gagtggccgc tggcataggc
    18661 gaagatggcg aggccggcgg caacgaggac gacggggaag gtcgcaaggc cgtccaagcc
    18721 gtcggtgagg ttgacggcat tggatgtgcc gacgatggtc aggtaagaca acaccaaaaa
    18781 gccgaccacg cccagcggca gggcgatttg tttgaagaac gggacaatca aaatattgtt
    18841 ggcggaattg gcggcaaggt aaaacaatgc caaaccggcg ataatggcaa cgcttgactg
    18901 ccacaccatt ttgaatttgg cggacacgcc gttggggtct ttatagacga ctttgcgcca
    18961 gtcgtcgtaa aaaccgagcg caccggtggc aagcagtacg cccaagagaa tccagatata
    19021 cgggttcgcc cagttgcccc acaacagggt ggacacggta atggcggtca gaatcagcga
    19081 accgcccatc gtcggcgtgc cgtttttgac gaggtgggtt tgcgggccgt cggtacgcac
    19141 tgcctgcccg catttgagcg cggtcagcct gcgtatcgtc cacgggccga acatcaggga
    19201 aaacgctaag gcggtcaacg ccgccatgac ggcgcggaat gtggtgtatt gaaaaatatt
    19261 cagaccggtt aaccagttgc tgaaatgtgc gagccataaa aacatggggc ttcctttttt
    19321 tgttttgt
//
LOCUS       DAWHRS010000019        19269 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWHRS010000019 DAWHRS010000000
VERSION     DAWHRS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.019"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..175)
                     /locus_tag="WIB82_000477"
     CDS             complement(<1..175)
                     /locus_tag="WIB82_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ3841827.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVL"
     gene            complement(300..1945)
                     /locus_tag="WIB82_000478"
                     /pseudo
     CDS             complement(300..1945)
                     /locus_tag="WIB82_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(1968..3446)
                     /locus_tag="WIB82_000479"
     CDS             complement(1968..3446)
                     /locus_tag="WIB82_000479"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ3841828.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVAGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(3471..5216)
                     /locus_tag="WIB82_000480"
     CDS             complement(3471..5216)
                     /locus_tag="WIB82_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ3841829.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(5277..5540)
                     /gene="ftsL"
                     /locus_tag="WIB82_000481"
     CDS             complement(5277..5540)
                     /gene="ftsL"
                     /locus_tag="WIB82_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ3841830.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(5537..6523)
                     /gene="rsmH"
                     /locus_tag="WIB82_000482"
     CDS             complement(5537..6523)
                     /gene="rsmH"
                     /locus_tag="WIB82_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ3841831.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRRIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(6520..6975)
                     /gene="mraZ"
                     /locus_tag="WIB82_000483"
     CDS             complement(6520..6975)
                     /gene="mraZ"
                     /locus_tag="WIB82_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ3841832.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVSAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            7254..8471
                     /locus_tag="WIB82_000484"
     CDS             7254..8471
                     /locus_tag="WIB82_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3841833.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHIIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYICEAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WVGFRFGDICHKL"
     gene            8603..8679
                     /locus_tag="WIB82_000485"
     tRNA            8603..8679
                     /locus_tag="WIB82_000485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8637..8639,aa:Met,seq:cat)
     gene            8687..8762
                     /locus_tag="WIB82_000486"
     tRNA            8687..8762
                     /locus_tag="WIB82_000486"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8720..8722,aa:Ala,seq:ggc)
     gene            complement(9053..9874)
                     /locus_tag="WIB82_000487"
     CDS             complement(9053..9874)
                     /locus_tag="WIB82_000487"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ3841834.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGLFILWVEKRQSWAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(9907..10551)
                     /locus_tag="WIB82_000488"
     CDS             complement(9907..10551)
                     /locus_tag="WIB82_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ3841835.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(10554..11399)
                     /gene="ftsN"
                     /locus_tag="WIB82_000489"
     CDS             complement(10554..11399)
                     /gene="ftsN"
                     /locus_tag="WIB82_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ3841836.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALYEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVGEKAQKKDAETVKKQAVKPSKETEKKASKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(11505..13001)
                     /locus_tag="WIB82_000490"
     CDS             complement(11505..13001)
                     /locus_tag="WIB82_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ3841837.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLE
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(13015..13335)
                     /locus_tag="WIB82_000491"
     CDS             complement(13015..13335)
                     /locus_tag="WIB82_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ3841838.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            13818..15344
                     /gene="putP"
                     /locus_tag="WIB82_000492"
     CDS             13818..15344
                     /gene="putP"
                     /locus_tag="WIB82_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ3841839.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSTAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRATR
                     "
     gene            complement(15434..15601)
                     /locus_tag="WIB82_000493"
     CDS             complement(15434..15601)
                     /locus_tag="WIB82_000493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841840.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            15593..19198
                     /gene="putA"
                     /locus_tag="WIB82_000494"
     CDS             15593..19198
                     /gene="putA"
                     /locus_tag="WIB82_000494"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ3841841.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRASRTKASGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYAGEAENTLPKDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVAHLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGIQAVVEPDSPLASYTADLEGLLLVNGKPETAGISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4536 a         5549 c         4687 g         4497 t
ORIGIN      
        1 ccaatacgtc ttcaacaaaa tcatgcgcat caaaccgccc gcccgccaat gcgaaaaaca
       61 catcgcccgc gcggatgtcg cggctgtcgg ttacgatgcg cgacacgggt ttgctttcag
      121 acggcatcgg aagcttgagg gcttggcaga tgaaatttag gtccagtggt ttcatattta
      181 ctttcgttaa tattcgggcg gcggacacat cggtagcggc tgattttttt atcgcctgtt
      241 ttgctgtggt aaaacacaga ttattttccc attctcattc ggcatttttt ctgtacgtat
      301 cattttttag acgtattttt agtcgatttg ccttttcccg cataccacgg cgcggggtcg
      361 tcgggcagtc cgtcgataaa ggcaaggtta ttgccttcgc cctgcacatc gggaacattc
      421 ccccaaaaat catagccgtc atcgggcaac tcgtcggttt cgatacccgt ccaactgccg
      481 aatccgcgta aaaaattaaa cgcgctgcgc ctcactttaa tcatttcgcg ccgcgtatca
      541 tcggacgaaa ttttaaccag cggcacgccg tagctttgac gcttccacgc accatgtgtc
      601 aacgttcgct cgccgccttt accgacatgc atcaaaccgt ggtcggaaaa gtaaaccaaa
      661 gaccagcttt ctttattttc attcaatatc ttaactgtat cttctaaaaa tttatcggtt
      721 tgcgcgatgg tggaaacata gcaggatatt ttttcagttt gatactgaaa ccgccgcgca
      781 tccttatcca aacgtgtgca aaaatcactg tgcgaaccca tcaggtgcat cacaatcagc
      841 cgaggcttcg tgcctgcatt tcctatcaaa acccgtttga acgccggcaa caaaaggcgg
      901 tcgctcaacc ccgcgctttt gccataatca cccctttggg taaaccacgg ataatcgctg
      961 cgtagggcat aggtggaaat ttcgttggca aaatgcccca acattccttg attagacagc
     1021 cacgccgtcc gaaaacccgc ctgcttcgcc aagctgacga tgttattgtt cggttctccc
     1081 ggcagcccca aagtctgcgg cagcgaaaga ttcgtcgcgt gggcggtcga ttggtaaccg
     1141 tttatcaaca gccctttggt ctgactcaaa aaaggcgtat cgggcaatgg gaaaccgtaa
     1201 acattcatat aatccgaacg cgcgctttca ccgataacca caacataatt tttatacttg
     1261 ggcgcaacat gacggatatg ccatgttgac gctttttttg ctgcttccaa aatgtgggcg
     1321 cgcttggcgg catattgtgc cggcgcggaa gccaaatcgt aatacaggtc gaaaatattc
     1381 aacaacaggc cggcatcagg ttctcgcaaa tctttatcgc tggcgatttt atccatcacc
     1441 gcgcaggaca aaatcaaagt caataatata gtcagccata ttttgctgcg gcgtttatag
     1501 ttttttacgt cagcaaatac ccccccgata cacaatatct ccaaactgtc aagccaaaaa
     1561 tgaaaaataa tgcctgcaca aaataaagcg acccgggaag attgccgaca aattcacgcg
     1621 cctcggcagg attgctttcc aatatcgaac cgactatctg ataagacggc gcaccataca
     1681 gccagccgac cggcaaatat agggcggttg tgccgacgta aatcagtaaa acaactgaag
     1741 aaacgcgcgg gaaaccgcgt gccaataata ataaaaatac aaccgaagcc aaagcaccca
     1801 cggcagaata acggtagccg tattcatatt ccaagtgata acctgatgcg atggccgcgc
     1861 caaaacaaaa ggctaaaagc gcgcaaatgc ccaatgtatt ttttaatgtc tgatccatat
     1921 tttttattcg ggcggattgt ttcatacgat gccgtccgaa tatcccatta tttacgagtt
     1981 aacaaagcct gtccgacgat ttcaagatcg gaaaaacggt gcttcacgcc ttgtacatcc
     2041 tgatagtttt catgcccttt gccggcaatc aggatgatgt cgtttgcggc ggcttgttca
     2101 accgcataac ggatggcggc ggcacggtcg gcttcgacgc attcgggcgc gggaacggca
     2161 ggcaggatgt cgttgatgat gtcgtgcgga ttttccaaac gcggattgtc gctggtgacg
     2221 acgactttat ccgcgccctg tacggctgcc gcgcccatca gcgggcgttt gccgcgatcg
     2281 cggttaccgc cgcaaccgaa tacgcaccac aaagccgcac cctgcggttt gatttcctgc
     2341 aaggtggcga gtgctttttc caatgcgtcg ggcgtgtggg cataatcgac aacgaccaaa
     2401 ggtttgccgc tgttcatgat gcagtccatg cgccctgaag cgggacggat ttttgccagc
     2461 acatccaata cattatcaag cggatagccg ttggcgcaca gcaaggcgat gcaggcggcg
     2521 aggttttgcg cgttgaaccg tccgagcagg cgcgtgcggc atttcccttc gccccacggg
     2581 gtttggaata cggctgctat gccgtctgaa gaggcggtaa agtcggtaat gcggatgtcg
     2641 gcgtgttcgc tgaaaccata gctgtaaacg gccaaatcgg gacagtcttt tttcagacga
     2701 cctacgagtt ccgcgccgta ttcgtcatcc acgttgatga ctgcgtgttt caagccgtgc
     2761 cagtaaaaca ggcgcgactt gatggcaccg taggcttcca tcgtgccgtg gtagtcgagg
     2821 tggtcgcggg tgaggttggt aaagattgcg ctgcggaatg acacgccgtt gacgcgcgac
     2881 tggtcaagcc cgtggctgga gacttccatc gcggcgactg ttgcgccttg ttgacggaaa
     2941 cggtagagca gggtttggac atcgacgggg gcgggtgtgg tatgcgtggt ttcttccaat
     3001 gcaccccaaa agccgttgcc gaccgtgccg acaatggcgg ttttttcgcc caacaaatcg
     3061 gcagcttgcg ccagccattg tgtgatggag gttttgccgt tggttccggc tacgccccaa
     3121 actttgaggc cgtctgaaac gttgccgtaa acttgcgccg ccaatatgcc ggcacggtgt
     3181 ttcaaatctt tgatgccttg attggggact ttccattcgg gattccacgc aaatttgccg
     3241 tcgtcgtccc aaaaaacaaa agccgcgccg ttggcaacgg cggcggggat ataactgcgt
     3301 ccgtcggcat attcgcccgg acaggcaacg aaaatatcgc cttgtttgat ttggcggctg
     3361 tctgaatgca acaaacgccc tgccgcgttt gcacacgaca gagtcgggat gccggtttca
     3421 gccaaagggg ttaacttgct gaacataaaa caatctcgtt gatactcgga ttaagacggt
     3481 gttttgacgg ctgcggcggt cagtggcttg gtcggggaaa tgcccaagat gttcaggctg
     3541 ccgcccataa tttttttgaa gggcggccct gccactacgc cgccgtaata accgtgggca
     3601 gtcggttcgt caatggttac cgccacaatc acacggggat ttttggcggg ggcaaaaccg
     3661 ataaaggtag cgatgtgttt gttgtcggca taacgcccgt tgacgaactt gcgcgccgtg
     3721 ccggttttcg cgccgacatc gaaaccgtcc accgcacccg ccgtaccggt gccgcccggc
     3781 tcggttacgg aaaccatcag attgcgtacc tcgcgcgcgg tcgattcttt gaatatgcgt
     3841 ttgccttgcg gcgcaaccgc ctgtttttca aagctgaccg gcagtaaaac gccgtcgtgc
     3901 gtcagtgcgg tataggcgcg cgccaattgc agcaggctca attgcaggcc gtaaccgaaa
     3961 gacatcgtcg cctgttcgat aggccgccac ctgcgccaat ttctcaacaa acctgcagtt
     4021 tcgcccggaa agcccgagtg catacgcaca ccgatgccca actcatgata gaagtcatac
     4081 atttcttcgg caccgaaacg cgcagacagt ttgcttgtgc cgacgttgga cgatttctgc
     4141 atgatgccgc gcacatccaa agaggggtaa acatgggtat cgcgcacggg agacggtccg
     4201 attttataag gctgcgtatt cagccgttcg ttcaaatcgg ttttgcccgc atccaatgcc
     4261 ttcgcaatca caaacggttt gattgccgaa ccgggttcga tcatatcggt tacggcacgg
     4321 ttgcgccgct gttcgctgtc tgcccggccg ggcctgttgg gatcgtaggc gggcgtattg
     4381 gccaaggcga ggatttcccc cgtgcgggca tccaaaacca ccaccgttcc ggcttttgcc
     4441 tgatggtatt cgaccgcctt gttcaactct tcataggcca aggtctgaat cctctgatcg
     4501 agggaaagga tgatgtcttt gccgtttttc ggggctttat tgcgcgggga gtccaagctg
     4561 tccacaatat tgccctgccg gtcccgcaaa acgacttccg cgccgtcttc gccatgcagg
     4621 ctgtcttcaa gcgaaagttc caaaccttcc tgacctttgc cgtcaatatc ggtaaatccg
     4681 atgacgtgtg caaacaggtt gcccatcggg taatggcgtt ttaattcttt ttcaaataca
     4741 aagttttcca aacccaaggc tttgacctct tcggcaacct tgggatcgag ctgccgctta
     4801 atccagataa acgacttgcc tttctgttcg agcttgttcc tcaaaacatc aaccggcaca
     4861 tcgacaagct cggacaggcg ttccaattgt gcggcagacg gcatttcctt catctcttta
     4921 ggcacggcaa acagggactc cgtcggcgca ctcaacgcca aaaccgcacc gttccggtcc
     4981 gaaaccgtac cgcgtgtagc cggcaatgtt tgagtccgca caatccggtt gtcgccctgt
     5041 tctttcaaaa agttatacgt taccgtctgc agatacagtc cgcgagcaat cagaccggca
     5101 aacaagaccg ctattgccat caggacgaag ctgatccgtc cgttactggt catcggcttt
     5161 ttgacctgct cttctttggg cagcatccga ggcttatatt cgctcttaat caacattttt
     5221 acttctcgtt attattatcc tgacgcagga atccgattcc ggcacacagg ctgcttctat
     5281 ctttgatgct ccaccataaa ggtattgccc gaaaccggcg gatggaggtt ttgtttttct
     5341 gccgccgccc tgatcgcttc gtggttcgcc aaacgcgcct gttgcagcct catttgcgca
     5401 taatcctgct ccaaggcgat ttcctgtttt ttcgccttat ccaaagctgt gaaattgagc
     5461 ctgtactggt tttgctgcat cacaacggaa aaagcggaaa cgcacaccgc aagcagcaga
     5521 aggaaattca atttgttcat tgccattcag acgtttttct ctgtgattgt tccggtatcg
     5581 gaccggcagt ccgctccgcc acacgcaaaa ccgcacttct cgccctcgga ttggcggcaa
     5641 tttccgcctc acccggcttt aatgccctgc ccacgatttt caggggcagc tcgggcaaat
     5701 ccgcttccct gaccgccgcc cagcgcggca ggggcgcgtg ttgcgaatat tttttgacaa
     5761 actgcttcac aatgcgatct tccaacgaat ggaaagcaat gaccgccaaa cgtccgccct
     5821 ctttcagacg acacatgacc tgcggcaata ctgcccctac ttcttcaagc tcgcggttaa
     5881 taaagatgcg gaccgcctgg aaggtgcgcg tcgcaggatc ctgcccccgc tcgcgagtac
     5941 ggacgttttg tgccacgatc tgcgccagct tgcgggttgt atcgattgga ctttccgccc
     6001 gttgcgcaac aatggcgcgc gcaatccggc ggctaaaccg ctcttcacca taattcttga
     6061 ttacctcgtg caaatcctgt tccgacgcaa ccgctatcca ctctgcggca gacataccgc
     6121 gcgtcgtatc catacgcata tccaaagggg catcgaaacg gaagctgaaa ccgcggctgc
     6181 cgtcatcgat ttgcggggac gaaatcccca aatcaaacag cgcaccgtcc accttgccga
     6241 tacccaaacc gtccaatgcc gtctgaaacg aagcaaaacc gccatgcacg acaccgaccc
     6301 gtttgtccga acgcgccagc tcttctgcca cagcaatcgc ctgcgggtct ttgtcgaaaa
     6361 caatcaaccg ccccgcatcg cccaaacgcg acaaaatcag ccgggaatgc cctcccctgc
     6421 cgaacgtacc gtccacatag acaccgtctt cgcgcacggc aagcgcatcc accgcctcat
     6481 tcagcaagac cgtgatatgc cggtaacttt ctgctccact cacaattgca aatccgtctg
     6541 actcaactgg aaggcaagtt cgtcaggatc gtcatccaaa gcctgaacca tctcagcctc
     6601 ccactgctcg cgaccccaaa gctccaaacg gttggcacga ccgaccaaaa cgacttcacg
     6661 gtcgaaatcc accctcttcc tcagtccggc agaaaccagc acccggccgg cgctgtccca
     6721 ttccaaaatt tccgcgttat gcagcaaaag attttgaaac cgccgcaaaa cagggttatc
     6781 cgccactttt aagtttaaaa gttgcgccgc aaccttttcc cactccgcaa cagggtacat
     6841 caacagcttg tgtttcgact cgagcgttac cactacggca ggcgtataga ggcgcgacag
     6901 aatgtcacgg aatttggcag gaacagccaa ccgcccctta ctgtcgatgc ttaattcgtg
     6961 tgcgccgccg aacatgacat gtcccaagcc gaaatcaaaa tcacaagggt aaaagagaca
     7021 ctttgcccca caattcccca ccaatcgaca ctataagaaa ttttaaacac tcggtcaaat
     7081 cagggcatga aaactcatta acatatctga aatttttatt ccttttaaaa acaataagat
     7141 aaaaaatgac gacaacggca cggcgggtgc ggtacagaat aatcgaacca ataaacaact
     7201 atatatgatt aatttaataa tataaacaca atatatagta ttaagataaa gccatgacag
     7261 cacccgtacc aacgtgtaat atgtcgggaa atccaataaa tttacacaag ctaacactta
     7321 tcatgcccct ccccctcccc tctcccgaag cacggcaatt ctcgctcaaa ctgcaaaccc
     7381 tcattgccga aaaaatcggc aaacacggca actggattcc attttcacgt tttatggaat
     7441 tggttttata cgctccgcaa tacggctact acaccggcgg cagccataaa atcggcaata
     7501 ccggggattt tattaccgca ccgaccctca cctctctgtt tgcacagaca ctggcacgcc
     7561 aacttcaaga acttctatcc caaacggcgg gcaatatcta tgaattcggc gcgggaaccg
     7621 gacagcttgc cgccgatttg ttgggcagca tttcggacgg catcagccgt tactatatta
     7681 ttgaaatatc gccggagctg gcagcacgtc agaaaaacct gattcaagca cgcgcaccgg
     7741 aagcatctca aaaagttgtc cacttgaccg cacttcccga agcgtttgac ggcatcatca
     7801 tcggcaacga agtactcgat gccatgcctg tcgaaatcgt ccgtaaaaat gaaggcggct
     7861 cattcgagca tgtcggcgtt tgcctagata atgaccgttt tacctattcg gcacgaccgc
     7921 tgcacgactt gcagctatct gccttggctt ccctctattt tcctcaaaca gattatccct
     7981 ataccagcga actacatccg caacaatatg cctttatccg cacccttgcc tcaagactcg
     8041 aacacggctg catgatattc atcgactacg gttttgatgc agcgcagtat taccaccctc
     8101 aacgcaatca aggtactctg atcggacact accgacatca cattatccac aatccttttg
     8161 acttcatcgg attggccgac ctgaccgcac atgtcaactt taccgacatt gcacaagcag
     8221 ggacggatgc cggattagat ttgataggtt accttcccca atcccatttc ttattgaact
     8281 tgggcattac cgagctattg gcacagacgg ggaaaacgga ttcggcagcc tacatctgtg
     8341 aagctgctgc cgttcagaaa ctgattgacc agcatgaaat gggcgaactg tttaaagtca
     8401 tcgcattcgg aaaaaatatc ggcatcgact gggtaggatt ccgcttcggc gacatctgcc
     8461 acaaactcta accctcatgc cgcctgaatc cgcttcagac ggcataaact ttttaacatt
     8521 taaaaacagt caactaattc aaaattaaaa aatacggctt gtcaaaaaaa cagaaaaaca
     8581 tataatagcg tcttcacgaa acggcgaatt agctcagtcg gttagagcag aggaatcata
     8641 atccttgtgt ccggggttcg agtccctgat tcgccaccaa attttcgggg gtatagctca
     8701 gttggtagag cgcttgcatg gcatgcaaga ggtcagcggt tcgatcccgc ttacctccac
     8761 cagataaaaa agcacagacc gtaaaaaggt atgtgctttt ttattacctg attaccggca
     8821 aataaagaat aaaccactgc cttcaaaaca ggcaatcgac tttaaaccta tcgccccgcc
     8881 tgtcctgatt ttatagtgaa ttaaatttaa accggtacag cgttggctcg ccttgccgta
     8941 ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatt cactatatca
     9001 gcccgccaga caaacccgac ccgaataatg tcttcaggtc gggtttatgg tttcattccc
     9061 aacttatcca gcctgacagc cacaatataa tgatggcaat accaaaaaca atgcggtaat
     9121 aggcaaaagg aatataattt ttcttggaaa caaacctcag caacgctttg accgccacca
     9181 agcctgaaac aaaggcagca acaaagccaa tcaaaatcaa accgacatca tgcagggtga
     9241 aaaaccggta atgcttcagg acatcataag ccgttgcggc aaccatcatc ggcacagcca
     9301 agaaaaacga gaattctgtc gcagttttcc gttcgattcc ccaaagcatc ccgcccataa
     9361 tcgtactgcc cgaacgggac gtacccggaa tcagtgcaaa cacttgggca acgccgatca
     9421 tcaaggcatc aatcggacgc aatgcatcaa catcggcaat tttaggctct gcccagcttt
     9481 ggcgtttctc cacccacaaa ataaacaaac cgcccaaaac cagcatgacc gccacactca
     9541 aaggattgaa cagatgttct ttaatctgtt tgccgaacaa cagccccatc acggcggcag
     9601 gtataaaagc aatggcaaga ttaaggacga aacggttggc tttccggtct tttcccacgc
     9661 catgcaacac attgctgaaa cgttgccggt attcaaacac taccgccaaa actgcaccga
     9721 gctggatggc aatttcaaaa accttgtgat tgctgtgaaa accaatcaga ttgccgaaca
     9781 caatcaaatg cccggtgctg gaaatcggta aaaattcggt aaaaccttct accaagccca
     9841 tcatcagggc tttcaggaca atcagaaaat ccattgcttg cgcttctttc ggatacggga
     9901 gttcggctat ttctgtacag caggggtctg acgcttgcgt tcttccctga ctttggcaac
     9961 caattcttta atcgtatgaa cgccgccgtc aaagccgtta ttgaagataa cgcggtattt
    10021 tccgccgaca ataacggtcg gcgtgctgtc aataccgtat tgttccgtca gtttctgcat
    10081 ttttaatgcg gcggcggcag cttcgggaga atcataggcg cgcatcagtt ttttgccgtc
    10141 aaagcctttt tgagacaaag cccactttcc ggcaaccgac ctgttttcca agcggatttt
    10201 ttgttcgtaa actgctttaa acacagcagg gtttgcctga tatttcaaac ccgacaaatt
    10261 gacggcggcc gccatcctgg ccagaccgag catttcaggc tgccagacca cgtgctccgt
    10321 cctcaaatag gcatcagacg gcaatgcctt gcccagtttc aataacaaag gatcgaaatg
    10381 atggcaatgt acgcagaaat agccgaaaaa ttccaaaacc tcaattttac ccgactgttc
    10441 ttgaggaatg ggtttatcca acacaagata gtcttcccct tccgtcaggg catatgcctg
    10501 cgcggacaac actgccgaca gcagcagcgg caacagatgt ttgagcttca taattatttg
    10561 ctttcgatag aacggatcag gctggcgact tcatgttttt tcaactcgtc ctgcattttt
    10621 ttcaccgcat cggcagacat attgccgctt tgcacccggt aaagcgtttt atgtcccgcc
    10681 tgataaccga ccaccttgga agatatgccc aagattgcca gtttggcacg ctgcccttcc
    10741 gcgctccggc ggtcggcata cgcgcccatt tgcagataat gcgttgcttc cgccttgtcg
    10801 gacgttttca ttttctgcac ttctttggcg gcggcactgc gcgctttttc gatgctgccg
    10861 ctgttgagga tttgttccgg ggtcggtttg ggtgcaactt tttccttttc cgcctttttc
    10921 tcttcttttg aagctttttt ctctgtttct ttagacggtt ttaccgcttg ttttttaacc
    10981 gtttcggcat ctttcttctg cgctttttcc ccgacggttt gttcacgctc ttccgtcagc
    11041 actttcttac gcactgcctg tccgtccggc tcttcccgtt ccggctcgcc cgccttttct
    11101 tcaacctcgt cggctttatc ggcagctttt tccgcatccg actgctctgc ctctgtcgca
    11161 gcatccggtt cgtacaaggc gttttgatcg gccggttcag gttggatgtc ttccttaggc
    11221 tggtttttcg gtttcaggat ttccgtttct gcaggctgct tcgacgaagc cgggattttg
    11281 aacgcatttt gaccgctctg gttcagataa aacaaaatac cggcaataat gaccgtcgcc
    11341 agtatcaaac cgaagaaaaa accggacaga ccttttccgg attgggaaaa tttgttcata
    11401 aacatacctt aatgtgtttc agacggcatt aacgccgttt gcttacgggc ggatattcta
    11461 acaatatcgc catatttggg caaaacctgc ttccattccc gttcctataa agcgcgacgg
    11521 aaacctatgg ctttcatgac gtggcttctg ccgacttctt cgtcgcccgc caaatccgcc
    11581 aatgtacgcg ccacgcgcat aatgcggtgg aagctgcggg cagaaaggga gagtttttcc
    11641 agcaggccgc ccaatgcttc ctgcgcttct ttttgaatgc gggcggatgt gtcgagttca
    11701 ctgacactca aggcggcatt cactttgcct tgccgtgcgt attgtttgtc tctagcggcg
    11761 ataacgcgtt ccaaaacgga cgcgctgctt tcgcccgctt cctgctgcat cagttcggcg
    11821 gcggacaggc tcgggacttc gatggtcaaa tcgatgcggt cgagcagcgg gccggaaatc
    11881 ttgctgcggt aacgcgcgac gctttcgggc gtgcagcggc agggcttgac gggatgcccg
    11941 agataaccgc acggacacgg gttcatggcg gcgacaagtt ggaatttcgc cggatagacg
    12001 gcttggcgcg ccgcgcggga aatgtggatt tcgccgtttt ccaaaggttc gcgcaacact
    12061 tccaaaactt tgcggtcgaa ctcgggcaat tcatccaaaa ataaaacgcc gtgatgcgcc
    12121 aatgaaattt cgcccggacg cggatccgaa ccgccgccga ccatagccgc cgcgctggcg
    12181 ctgtgatgcg gactgcggaa aggacggttg ctgtcgagtt gttgctggtg gttgggcagg
    12241 agcgaacgca atgcccaaac ttctaccaat tcgtcttcgg tcagcggcgg caggatgccg
    12301 ggcagccgtt gggagagcat agacttgccc gttcccggcg gacccatcat caagaggctg
    12361 tgtccgcctg cggcagcgat ttccaaagca aggcgcgcgg tgtgctgacc tttcacatcg
    12421 cacaaatcag gttgtccgcc atgttcaaac ggcatctgag gaacttggca ttcggtttgc
    12481 gccaaaggtt cgatgccgtt caaatgggcg gcgacttcgc ccaaagagcg cgcgccgtaa
    12541 acggtaatgc cgcgcatcac ggcggcttgt cctgcatttt cttcaggcaa aacaaatgca
    12601 cgttttgcct gcataccctg ccacgccatc gccaacgcgc cacgcacggg gcgcaacagc
    12661 cccgacagtg ccaattcccc cgcaaactcg tattcctcca gtttttcggg cgcaacctgc
    12721 cccgatgcgg caaggatgcc gattgcaatc ggcaaatcga aacgccccga ctctttgggc
    12781 aggtcggcgg gggcgaggtt gacggtgatt tttttggcgg ggaattcaaa accgctttga
    12841 ataatggcgg cacggacgcg gtcgcgactt tctttgactt ccgtatcggg cagcccgacg
    12901 atgttgaaat gtggcaggcc gttggcaagg tgggcttcca cttcgaccaa cggcgcattc
    12961 ataccgctca aggcgcggct gtaaaccaag gcaagcgaca tatttcagac ggccttattc
    13021 gccggcttcg gtttgctgcc tgatttcggc gacggcttct tcggcagcgg cttcagccgc
    13081 ttccaatgct gcccgttcgg gattttgcgc ggcttcgagt tttgccaaac gcgcttccaa
    13141 agccgccagt ttggtacgag ttttgattaa aacctgctgc tggatgtcga attcttcgcg
    13201 cgtaaccaga tccatacggt tgaacgcgcc gcccagcatc gccttaatat ttttttccac
    13261 atctttggca gggctgttgg cgatggtttc gctgattttc gaaccgactt cttcaaaaag
    13321 ctgcttgccg aacataatct gtatccttcc tgaacatatc aaattcaatc ggctattgta
    13381 taaggaaaaa tgccgtctga aaacgggcga cggataatcg gcaaaacata ccgcgcctcc
    13441 tttgcggttg ctaaattaca cctcaaaaca cctgccgcct gaaaacggat ttcagattcc
    13501 gcgcaacgcc tgattacaag acactacaaa acaatatgct gttttaaatg atttttccga
    13561 cgcgcatcgt ttcaaactcg gctttttaag ccattaagtg ctttgcaaac aacagaaatt
    13621 gggttatgct gaaacggatt atttacaatt tcatatagtt ttattacata tcttattgtg
    13681 attgaagata atttatccga atcccccttt cgggtatccg gattttccgt tgtactttta
    13741 ttagaaaaac atttcaggcg caagttgctt gcaatttcaa agcctgtaat tctgaagttt
    13801 tagacgagga atagcacatg aatcccatgt acattacttt tgcaatctat ctggttgcag
    13861 tccttctcat cgggcttgcc gcctattttt ctacgcgcaa tttcgacgat tatattttgg
    13921 gcgggcgcag cctaggcccg tttgttaccg cgatgtcggc aggcgcgtcc gatatgtccg
    13981 gctggctgct gatgggtctg ccgggcgcga tttatttgag cggtttgaat gaggcttgga
    14041 ttgccatcgg cctcttggtc ggcgcgtatt tcaactggct tttggtggcg ggccgtctgc
    14101 gcgtgcatac cgaatacgcc aacaatgcgc tgacgctgcc ggattatttc ttccaccgct
    14161 tcggcgcggg cggacacttg atgaaagtgg tttccgcatt gattatcctg ttcttcttca
    14221 cgatttattg cgcctcgggc attgtggcgg gcgcaaccct gttccaaagc ctatttgaag
    14281 gtatgactta caatcaggca atgtggctgg gcgcgggcgc gaccatcgcc tataccttct
    14341 tgggcggttt cttggcggtc agctggacgg atacgctgca ggcttctttg atgattttcg
    14401 ccctgatttt aacgccggtg atggtctatc tgggcttggg cggcgcggaa cagatgtcta
    14461 ccgcgattca aagcgtcgcc gcaggcacgg gcaaagaata cggcagcctg tttgccggta
    14521 cgaccgtcat cggcatcatt tccaccgccg catggggctt gggctatttc ggacagccgc
    14581 acattttggc gcgctttatg gcggccgaaa gcgcgaaatc gctggtatcc gcacgccgca
    14641 tcggtatgac ttggatggcg ttgtgccttg cgggcgcggt agcggtcggt tatttcggca
    14701 ttgcgtattt cggtgcaaat cccgacaaag tttcttctat gagcggcaac cacgaacgca
    14761 tcttcatcgc gctttccacc ctgctgttca acccttggat cgccggcatc attttgagcg
    14821 cgattttggc ggcggtgatg tccaccctgt cctgccagct tttggtttgc tccagcgcga
    14881 ttaccgaaga cttctacaaa ggctttttgc gtaaaaacgc gcaacagtcg gaattggtat
    14941 gggtcggccg cctgatggtc ttggcgattg ccgtgatttc catcctgatt gcttccgatc
    15001 cgaacagcaa agtattggga ctggtgtctt acgcgtgggc aggtttcggc gcggcattcg
    15061 gcccgattgt gattctgtcc gtattgtgga aacgcatcac tgcctacggc gcgttgtcgg
    15121 gtatggttgc aggtgcgtta accgtagtcg tatgggcgga atgggtgaaa aaaccggctc
    15181 aagcggcagg agaaagcggc ttgttgacga tgtacgaaat cgtgccgggc tttattgttt
    15241 gcttgatcgt tgccgtgttg gtttccctgt tcaacaaaga accttcgcgc gaaacccaag
    15301 aacgctttga aaaagccgac gcagattacc gcgccacccg ataaacccct ttcagacggc
    15361 acggcatcct ggttcgtgcc gtctgaaccc gtttccgggt aaaacacaaa taaaccttgc
    15421 ctgcatacag accttatttg tgttttaccc gtttcatttt caaagaaacg gaagcaccta
    15481 ccctgcccgc atttgaagcc ttgtatttca tctgcggatt tattacgata ttaccgtatt
    15541 caggccgcac cgatgccgcc tgccccccga aaaactttgg agaatccaaa aaatgtttca
    15601 ttttgcattt ccggcacaaa ccgccctgcg ccaagcgata accgatgcct accgccgtaa
    15661 tgaaatcgaa gccgtacagg atatgttgca acgtgcacag atgagcgacg aagagcgcaa
    15721 cgccgcctcc gagcttgccc gccgtttggt tacccaagtt cgtgccagcc gcaccaaagc
    15781 cagcggcgta gatgcgctga tgcacgagtt ttcactctcc agcgaagaag gcatcgcgct
    15841 gatgtgtctg gcagaagccc tactgcgtat ccccgacaac gccacgcgcg accgcctgat
    15901 tgccgacaag atttcagacg gcaactggaa aagccatttg aacaacagcc cttccctctt
    15961 cgtcaatgcc gccgcctggg gcctgctgat taccggcaaa ctgaccgcca caaacgacag
    16021 acaaatgagt tccgcactca gccgcctgat cagcaaaggc ggcgcaccgc tcatccgcca
    16081 aggcgtaaat tacgccatgc gcctgttggg caaacagttc gtaaccggac agaccattga
    16141 agaagccctg caaaacggca aagaacgcga aaaaatgggc taccgcttct ccttcgatat
    16201 gttgggcgaa gccgcctaca cccaagccga tgccgaccgc tactaccgcg actatgtcga
    16261 agccatccac gccatcggca aagatgcggc aggacaaggc gtttacgaag gcaacggcat
    16321 ttccgtcaaa ctttccgcca tccatccgcg ctactcgcgc gcccaacacg gccgtgtgat
    16381 gggcgaactg ttgccgcgcc tgaaagagct gttccttttg ggtaaaaaat acgatatcgg
    16441 tatcaacatc gatgccgaag aagccaaccg tctggagctg tctttggatt tgatggaggc
    16501 tttggtttca gaccctgact tggctggcta caaaggtatc ggtttcgttg tccaagccta
    16561 ccaaaaacgt tgtccgttcg ttatcgacta cctgattgac ctagcccgcc gcaacaacca
    16621 aaaactgatg atccgcctcg tcaaaggcgc gtattgggac agcgaaatca aatgggcgca
    16681 agtggacggc ttgaacggct atccgaccta cacccgcaaa gtccacaccg acatctccta
    16741 cctcgcctgc gcgcgcaaac tgctttccgc gcaagacgcg gtattcccgc aatttgccac
    16801 gcacaacgcc tacaccttag gcgcaatcta ccaaatgggc aaaggcaaag actttgaaca
    16861 ccaatgcctg cacggcatgg gcgaaaccct gtacgaccaa gtcgtcggcc cgcaaaactt
    16921 aggccgccgc gtgcgcgtgt acgccccggt cggcacacac gaaaccctgc tcgcctactt
    16981 ggtgcgccgc ctgttggaaa acggcgcgaa ctcgtctttc gtcaaccaaa tcgtcgatga
    17041 aaacatcagc atcgacacgc tcatccgcag cccgttcgac accatcgccg aacaaggcat
    17101 ccacctgcac aacgccctgc cgctgccgcg cgatttgtac ggcaaatgcc gtctgaactc
    17161 gcaaggcgtg gacttgagca acgaaaacgt attgcagcag cttcaagaac agatgaacaa
    17221 agccgccgcg caagacttcc acgccgcatc catcgtcaac ggcaaagccc gcgatgtcgg
    17281 cgaagcgcaa ccgattaaaa atcctgccga ccacgacgac atcgtcggca cagtcagttt
    17341 tgccgatgcc gcgcttgccc aagaagcggt tggcgcagcc gttgccgcgt tccccgaatg
    17401 gagtgcgaca cctgccgccg aacgcgccgc ctgcctgcgc cgtttcgccg acctgctcga
    17461 acagcacacc cccgcgctga tgatgcttgc cgtgcgcgaa gcaggcaaaa cgctgaacaa
    17521 cgccattgcc gaagtgcgcg aagccgtcga tttctgccgc tactacgcag gcgaagccga
    17581 aaacaccctg cccaaagacg ccaaagcagt cggcgccatc gtcgccatca gcccgtggaa
    17641 cttcccgctc gccatcttta ccggcgaagt cgtttccgca ttggcggcag gcaacaccgt
    17701 catcgccaaa cccgccgaac aaaccagcct gattgccggt tatgccgttt ccctcatgca
    17761 cgaagccggc atcccgactt ccgccctgca actcgtcctc ggcgcaggcg acgtgggtgc
    17821 ggcattgacc aacgatgccc gcatcggcgg cgtgattttc accggctcga ccgaagtggc
    17881 gcacctgatc aacaaagccc ttgccaaacg cggcgacaat cccgtcctga ttgccgaaac
    17941 cggcggacaa aacgccatga ttgtcgattc caccgcactt gccgaacaag tctgcgccga
    18001 cgtattgaac tccgccttcg acagcgcggg acaacgctgc tccgccctgc gtatcttgtg
    18061 cgtccaagaa gacgttgccg accgtatgct cgacatgatc aaaggcgcga tggacgaact
    18121 cgtcgtcggc aaaccgattc agctcactac cgatgtcggc cccgtcatcg atgccgaagc
    18181 acagcaaaac ctgttgaacc acatcaacaa aatgaaaggc gttgccaagt cttaccacga
    18241 agtcaaaacc gctgccgatg tcgattccga aaaatccacg ttcgtccgcc ccatcctgtt
    18301 tgaattgaac aacctcaacg aactgcaacg cgaagtcttc ggccccgtcc tgcacgtcgt
    18361 ccgctaccgc gccgacgaac tcgacagcgt catcgaccaa atcaacagca aaggctacgc
    18421 cctgacccac ggcgtacaca gccgcatcga aggcacggta cgccacatcc gcagccgcat
    18481 cgaagccggc aacgtttacg tcaaccgcaa catcgtcggc gcagtcgtcg gcgtacagcc
    18541 cttcggcgga cacggtctgt ccggcacagg tcccaaagca ggcggttcgt tctacctgca
    18601 aaaactgacc cgcatccccg aatgggttgc cccgaccctg agccaaatcg gacaggcaga
    18661 cgaagccgcg ctcaaacgcc tcgaagcact ggttcacaaa ctgccgttca atgccgaaga
    18721 gaaaaaagcc gcagcggccg ctttgggaca cgcccgcatc cgcaccctgc gccgtgccga
    18781 aaccgtcctt accggaccga ccggcgagcg caacagcatc tcatggcacg cgcccaaacg
    18841 cgtttggata cacggcggca gcacggttca agcctttgcc gcactgaccg aacttgccgc
    18901 ctccggcata caggcagtgg tcgaacccga cagccccttg gcttcctaca ctgccgactt
    18961 ggaaggtctg ctgctggtca acggcaaacc cgaaaccgcc ggcatcagcc acgttgccgc
    19021 cctgtcgcct ttggacagcg cgcgcaaaca ggaacttgcc gcccacgacg gcgcactcat
    19081 ccgcatcctc ccttcggaaa acggactcga catcctgcaa gtgtttgaag aaatctcttg
    19141 cagcgtcaac accacagccg ccggcggcaa cgccagcctg atggcggtcg ccgactgatt
    19201 ttgccgaaat acccgggcgc ggcccgtgaa ccaatgccgt ctgaaaacct ttcagacggc
    19261 atttttata
//
LOCUS       DAWHRS010000020        18984 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWHRS010000020 DAWHRS010000000
VERSION     DAWHRS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18984
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.020"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(59..952)
                     /locus_tag="WIB82_000495"
     CDS             complement(59..952)
                     /locus_tag="WIB82_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ3841842.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALAGRLKRINHTVNVHTVNEKLDGCRL
                     TDLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGTER"
     gene            995..3697
                     /gene="ppc"
                     /locus_tag="WIB82_000496"
     CDS             995..3697
                     /gene="ppc"
                     /locus_tag="WIB82_000496"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ3841843.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNGDVMALSDKSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            3820..4809
                     /locus_tag="WIB82_000497"
     CDS             3820..4809
                     /locus_tag="WIB82_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ3841844.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            4864..5193
                     /locus_tag="WIB82_000498"
     CDS             4864..5193
                     /locus_tag="WIB82_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841845.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            5204..5509
                     /locus_tag="WIB82_000499"
     CDS             5204..5509
                     /locus_tag="WIB82_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ3841846.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            5515..5694
                     /gene="ssrS"
                     /locus_tag="WIB82_000500"
     ncRNA           5515..5694
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WIB82_000500"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5872..6303
                     /gene="rplM"
                     /locus_tag="WIB82_000501"
     CDS             5872..6303
                     /gene="rplM"
                     /locus_tag="WIB82_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ3841847.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            6313..6705
                     /gene="rpsI"
                     /locus_tag="WIB82_000502"
     CDS             6313..6705
                     /gene="rpsI"
                     /locus_tag="WIB82_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ3841848.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(6960..7889)
                     /locus_tag="WIB82_000503"
     CDS             complement(6960..7889)
                     /locus_tag="WIB82_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ3841849.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            8020..8769
                     /locus_tag="WIB82_000504"
     CDS             8020..8769
                     /locus_tag="WIB82_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ3841850.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            8892..9473
                     /gene="petA"
                     /locus_tag="WIB82_000505"
     CDS             8892..9473
                     /gene="petA"
                     /locus_tag="WIB82_000505"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ3841851.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            9492..10841
                     /locus_tag="WIB82_000506"
     CDS             9492..10841
                     /locus_tag="WIB82_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ3841852.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            10856..11644
                     /locus_tag="WIB82_000507"
     CDS             10856..11644
                     /locus_tag="WIB82_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ3841853.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            11983..13767
                     /gene="rmuC"
                     /locus_tag="WIB82_000508"
     CDS             11983..13767
                     /gene="rmuC"
                     /locus_tag="WIB82_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ3841854.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRLQGEFADLNAQLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(13973..14605)
                     /locus_tag="WIB82_000509"
     CDS             complement(13973..14605)
                     /locus_tag="WIB82_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ3841855.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            14697..15521
                     /locus_tag="WIB82_000510"
     CDS             14697..15521
                     /locus_tag="WIB82_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ3841856.1"
                     /translation="MIKKTIGGIIPIFMAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISAAVRS
                     ASSDWRGIRLHVFDVPKAQGNLYRRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRKAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            15590..16153
                     /locus_tag="WIB82_000511"
     CDS             15590..16153
                     /locus_tag="WIB82_000511"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ3841857.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            16234..16671
                     /locus_tag="WIB82_000512"
     CDS             16234..16671
                     /locus_tag="WIB82_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ3841858.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            16740..17009
                     /locus_tag="WIB82_000513"
     CDS             16740..17009
                     /locus_tag="WIB82_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ3841859.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            17009..18784
                     /gene="ptsP"
                     /locus_tag="WIB82_000514"
     CDS             17009..18784
                     /gene="ptsP"
                     /locus_tag="WIB82_000514"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ3841860.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTARRQLAERGDAFGDVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQINSVSVEEEADFKGRK"
BASE COUNT         4654 a         5067 c         5234 g         4029 t
ORIGIN      
        1 atttttaaaa ccgttccgcc gcatccgtct ttatgccgtc tgaaacggcg gcagggtttt
       61 accgttccgt gccgcaaatc gggcattcgg ggttgcgcgg caggtcgaaa tattgccagc
      121 ccccttccaa ggcacggtaa accgccagcc tgccgtgcga cggttcgccc gcatccagca
      181 ggattttcag agcctccgcc gcttgggtac tgccgatgat gccgaccagc ggcgagaata
      241 cgccgaagag ggaacagatg ccgtctgaag ccgatccgcc gtcaaacagg caggcgtaac
      301 acggcgagtc gggcaagtcg ggacggtaca cggcaagttg cccttcaaag cgcaccgctg
      361 ctcctgaaac cagcggtgtt ttcgtttgca cgcaggcacg gttgacggct tgccgcgtgg
      421 cgtagttgtc gcagcagtct aaaacgatgt cggcggcttg aaccaaatcg gtcaggcggc
      481 agccgtcgag tttttcgttg acggtgtgga cgttgacggt atggttgatg cgtttcaggc
      541 ggcctgccaa ggcttcggct ttgggcttgc cgacatcgcc ctcgtcaaat gcgacttggc
      601 gttgcaggtt gtgcagttcg accgtgtcgg aatcggctat ggtcagcgtg ccgatgccgg
      661 aagcggcgag atagggcagg gcggcggcac ccaaaccgcc gcagccgacg accaaaatat
      721 gcgcggcgga aagtttctgc tgcccttcga tgccgatttc gtccaagagg atgtggcggc
      781 tgtaccgcag caggagttcg tcgtcgttgt cgtgttcggt cgtggtcatg atgatgttcg
      841 gaaaaaacag ttgcgggcga ttgtaacgct gccgtcgggc ggcgttcaac ttcagacggc
      901 atttcgggac acgggcggtt aaagtgtgaa cggtttggca cggatgcggc atttgaggta
      961 catttacagt atttacggca atgagagaaa aatcatgcaa ctgcatattc tgaacaatcc
     1021 gaaggacgcg gctttggcgg cggacgcgga atttctgaaa caatccctgt tcaacctcct
     1081 gcacgaagaa gcctcgccgt tggttgtcga aacagtcaaa ctcttgtcca cttccgacga
     1141 cagcgcggca ttgattgaaa aagtattgcc gcaattggac gaacaacaaa cccacgattt
     1201 aaccttggcc tgcggcctgt tcgcccagat tttgaacatc gccgaagacg tgcaccacga
     1261 acgccgccgc caaatccacg aagaagccgg acgcggcggc gcggaaggca gcctgacgga
     1321 aaccgtccgc aggctcaaag cggggaaagc cgacggcaaa tcggtgcagc ggcagttgga
     1381 caatacgtcc gttaccgccg ttttgaccgc gcacccgacc gaagtgcaac gccaaaccgt
     1441 cttaagcttc aaccgccgca tccgcgcact gttgccgcaa cgcgaacgct gcaccaatgc
     1501 cgacgcgctg gcacggctgc gccgcgaaat cgacactatc ctgctgggct tgtggcagac
     1561 cagcgaaacg cgccgccaca aactcagcgt caacgacgaa atcaacaacg gcgtgtccat
     1621 cttcccgatg agcttttttg aagccctgcc caaactctac cgcaagatgg aacacgactt
     1681 tcagacggcc tatcccgatg tccgcgttcc gaacatcctc aaaatcggcg gctggatcgg
     1741 tggcgaccgc gacggcaatc cgttcgtttc tgccgaaacc ctgcgctttg cgttcagacg
     1801 gcatgccgat gccgtgttcc gtttttaccg cagcgaactc gacaaacttt accgcgaact
     1861 gccgctctcc atccgccgcg tcaaagtcaa cggcgatgtg atggcgttgt ccgacaaatc
     1921 gcccgacgaa gaaatcgccc gcacggaaga accctaccgc cgcgccatcg cctacatcat
     1981 ggcgcgcgcc atgggcaaag cgcgcgcgct cggtttgggt atgggttgca aattcggctt
     2041 cctcgagcct tatgcttcgg cacaagaatt tctcgatgat ttgaaaaaat tgcaacgttc
     2101 ccttatcgac aacggcagcc gcctgcttgc cgaaggccgt ttggcagacc tcatccgttc
     2161 cgtgtccgtg ttcggcttcc acatgatgcc gctcgacctg cgccaacacg caggcaaaca
     2221 cgccgatgtg gttgccgagc ttttccaaca cgcaggcttg gaagactaca acagcctgaa
     2281 cgaagagcaa aaacaagccg tcctgttgcg cgaattgagc catcaacgtc cgctgtacag
     2341 cccgttcatc acatacagcg accatacccg ccacgaactg gcgattttta acgaagcgcg
     2401 caaaatcaaa gacgaattcg gcgaagatgc cgttacacaa agcattattt ccaactgcga
     2461 acaacccagc gacctgcttg ccttggcatt gctgctgaaa gaaagcggcc tgttggcggt
     2521 ggaaaacggc aagccgcgca gccgcatcaa tatcgtgccg ctgtttgaaa ccattgaagc
     2581 gttggaaaac gcctgtccgg ttatggaaac catgttccgc ctcgactggt acgatgccct
     2641 gctcgaaagc cgcggcaata tccaagaaat catgctcggc tactccgact ccaataaaga
     2701 cggcggctac gttaccagct catggtgtct gtatcaggcg gaattgggct tggtcgaact
     2761 cttcaaaaaa tacgatgtcc gcatgcgcct gttccacggc cgcggcggca gcgtcggacg
     2821 cggcggcggc ccttcttacc aagccattct cgcccaaccg gcgggcagcg tggcgggaca
     2881 aatccgcatc accgaacaag gcgaagtcat taccgccaaa tacgccgacc ccggcaatgc
     2941 ccaacgcaac ttggaaaccc tggttgccgc gactttggaa gccagcatcc tgccggataa
     3001 aaaagaccct gatgccaaac tgatgcaggc attgtcggac gtatcgttca aatactaccg
     3061 cgaactgatt acccatcccg acttcatcga ctactttctg caaaccagcc cgattcagga
     3121 aatcgccacc ctcaacctag gcagccgtcc cgccagccgc aaaaccttgg cgcggattca
     3181 ggacttgcgc gcgattccgt gggtattttc ctggatgcaa aaccgcctca tgctgccggc
     3241 ttggtacggt ttcggcagcg cggtggaaac cttgtgcgaa gacaaacccg aaacgctcgc
     3301 cgccctgcgc gaacacgccc aaagcaaccc gttcttccaa gccatgcttt ccaatatgga
     3361 gcaagtgatg gcgaaaaccg acatcaccct cgcggaaaac tatgccggct tgagcgaatc
     3421 gccggaaaaa gccgccgtca tcttcggcat gattaaggaa gaataccaac gcagccgcaa
     3481 agccctgctc gacctgctgc aaaccgaaga acttttacgc gacaaccgca gcctcgcccg
     3541 ttcgcttgcc ttgaggattc catatttgaa cgcgctcaac ggcctgcagg tcgccatgct
     3601 caaacgcctg cgtaaagaac ccgacaatcc gcacgcgctg ctgatggttc acctgaccat
     3661 caacggcgtg gcgcaaggtt tgcgcaatac aggctgatag tgccgcatcg gggcaaaatg
     3721 ccgtctgaac gcctttcaga cggcatttcc ctgaccgcac ttgcagagaa acaccgattg
     3781 ttttaaagtg aacggcagtg atatgttgaa agacgaccaa tgaaaattac cgttatcggc
     3841 gcaggttcgt ggggtacggc gctcgccctg catttttccc aacacggcaa ccgcgtatcc
     3901 ctgtggacgc gcaacgcaga ccaagtccgc caaatgcagg aagcgcgtga aaacaaacgc
     3961 ggactgcccg gcttttcctt tcccgaaacc ttggaagtgt gtgcggattt ggcagacgcg
     4021 ctcaaagaca gcggacttgt ccttatcgta acctccgttg ccggattgag aagcagcgca
     4081 gagctgctca aacagtacgg cgcgggacac ctccccgtcc tcgccgcctg caaaggattc
     4141 gagcaggata cagggctgct gacctttcaa gtattgaaag aagtattgcc cgacaataaa
     4201 aaaatcggcg tactttccgg tccgagtttt gcacaggaac tcgccaaaca actgccctgc
     4261 gccgtcgtcc tcgcctccga aaaccaagag tggattgaag aactcgtacc gcagctcaac
     4321 acgaccgtca tgaggcttta cggcagtacc gacgttatcg gcgtggcagt cggcggtgca
     4381 gtcaaaaacg tcatggcgat tgccaccgga ttgtccgacg gtctggagta cgggcttaac
     4441 gcccgtgccg cactggttac acgcggatta gctgaaatca cccgccttgc ctccgcaatg
     4501 ggcgcacagc ccaaaaccat gatggggctt gctggcatcg gcgacctcat cttgacctgc
     4561 accggcgcac tctcacgcaa ccgccgcgtc ggcttgggtt tggcagaagg caaggaactg
     4621 catcaggtgc tggtcgaaat cggacacgtt tccgaagggg tcagcacgat agaagaagtc
     4681 ttcaatactg cctgtaagta ccaaatcgac atgccgatta cccaaaccct gctgcaactc
     4741 atccgcaaag aaatgacccc tcagcaggtc gtcgaaagac tgatggaacg cagcgcgcgt
     4801 ttcgaataaa caacagacag atgccgtctg aagccttcag acggcatacg gacaggtaag
     4861 gttatgaaac aaaatatcga aaaactcgaa agcagcgttt atacgctggt acaaaaattc
     4921 gaaaccctcg tcagcgaaaa ccgccgcctc aaagaaaccg tcgccgaact caaacgggcg
     4981 cacgagcggc aaaaactcga acacgaaacc gccgtcgacg aactcagcga agccctgctc
     5041 gtccaagtcg gcaaactcaa agaagacctg caaaacaaaa ttgacagcct gacagaagaa
     5101 aatacacgat accgcagcct gctcgaacag agcagggaaa aaatcagcgc actggcagcg
     5161 cgcctccccc aacggcagga aacgcagcaa taaggattaa aggatgaaca tcgaacaagt
     5221 ccacatcgaa gtcatgcacg cccggctgac cgtcaacacg ccggcagaag aaaaagacac
     5281 actgttgcag gcagtcggaa tgctcaacgg caaagccgaa gccatccgcg aaggcggacg
     5341 cgtcgcggac agcgaaaaaa tcgtcattat ggccgcgctc aacgtcgtcc acgacctgtt
     5401 gaaaacctcc ctgaacggcg gcgatttggc aatcggcgat tttgcgcgta aaatagccga
     5461 tatggacaac gcctgccaaa aagcactatc ccgcttggcg caggaataac ccctttttcc
     5521 ctgcggtgtc cgcgaaggca tatattcctt tgaaccaata agttcgctta aggttgccgg
     5581 gagcagtagt gggcgcgagc gtccttttgc ggacgcaccc gaaactaccc gaggcagccg
     5641 ccttgtcagc aaggttcaag cggattcggc cgaaacggct ctcgcgggga ttcccattca
     5701 aaaccgcatc gcaacgatgc ggtttttcac attggccgcc gccccgcccg aaaaagcagt
     5761 atgcagtctg ccgaaatccg gaaaatcctt ccgtaacgcg gtaatgcgat tgacaaaatc
     5821 gaatcctgtc tttaaaattt acaactttct atatctatct ggatttccac tatgaaaacc
     5881 ttttcagcga aaccccacga ggtgaagcgc gaatggttcg tcatcgatgc ccaagacaaa
     5941 gtcttgggtc gcgttgcggc cgaagtcgcc agccgtctgc gtggcaaaca caaacctgaa
     6001 tacacccccc acgtcgatac cggcgattac atcattgtta tcaatgcgga caaactgcgt
     6061 gtaaccggtg ccaaattcga agataaaaaa tacttccgcc attccggttt cccaggcggt
     6121 atctacgaac gcactttccg cgaaatgcaa gagcaattcc cgggccgcgc tttggaacaa
     6181 gctgtaaaag gtatgctgcc caaaggtcct ttgggttacg ccatgattaa aaaactgaaa
     6241 gtgtatgcgg gtgcggaaca cgcccatgct gcgcaacaac ccaaagtttt ggaactgaaa
     6301 taaggacgcg acatgaacgg taaatactac tacggcacag gccgccgcaa aagttcagtg
     6361 gctcgtgtat tcctgattaa aggtacaggt caaatcatcg taaacggccg tcccgttgac
     6421 gaattcttcg cacgcgaaac cagccgaatg gttgttcgcc aacccttggt tctgactgaa
     6481 aacgccgaat ctttcgacat caaagtcaat gttgttggcg gcggcgaaac cggccagtcc
     6541 ggcgcaatcc gccacggcat tacccgtgcc ctgatcgact tcgatgccgc gttgaaaccc
     6601 gccttgtctc aagccggttt cgttactcgc gatgcccgcg aagtcgaacg taaaaaaccg
     6661 ggtctgcgca aagcacgccg tgcaaaacaa ttctccaaac gttaatgttg gaaattcaaa
     6721 aaaccctgct tatcgcaggg ttttttattt gtagtagacg gtttcccatt ggcaatctaa
     6781 agattacaga ttgggcaaaa atcaaaaaca gtatagtgga ttaacaaaaa tcaggacaag
     6841 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc gtactggttt
     6901 ttgttaatcc actatacatg aaaaaataga aaaactgccg ggtatggttg aataaggggt
     6961 cagaccggtt ccagttcgct cagtccgggc aaatctgcaa aaccgcgttc gcgtatgatt
     7021 tggcaaaaat tgttcagata gctcttgtcc gtatcttcgg tacggatggc ggcatacagt
     7081 ttgctttgca gtccgtcggc agtaatttgg cggtggacga catagccttt ttcaaggtag
     7141 ggcatgactg tccaataggg aagggcggca atgccacgtc tgctggcaac cagttggata
     7201 atggcgatgg tcagttcgct gtgtcggcgc ggcgggttga tgtttttcgg aatcaggatt
     7261 tttttgggca aatccagcat ctcgtcggga acgggataag taatcagggt ttccccgata
     7321 aagtcttccg ccgtccaaac gtttttggcg gcaagcggat ggtctggtgc gcaaatgccg
     7381 accatttcgt aggcaaacag cggttggaaa gaaataccgt tttgtttttc cgcttcggaa
     7441 acaatggcaa ggtcggcacg gtgttgcagc agcagtccga cgggatccgc ttggaatccc
     7501 gatacgatat ccaattcgac ttggggccac atcgggcgga attcgcccat ggcgggcatc
     7561 agccagtcga aacaggtatg gcattcgacg gcaatccgca gctctcccgc ctccccttct
     7621 gtgattcgcg ccaaatctcg ttctgcaaca gcaacttgag gtatcagttc gtgggcgagg
     7681 cgcagcagcc tttcgcccac cggggtaaag cgcaagggcg tggatttgcg ttcgaacagc
     7741 ggcgtgccgt agtggttttc gagcatacgg atctggtggg aaagggcgga ttgggtaagg
     7801 aaaacccgtt tggcggcaag ggagacgctg ccggtttctt caagtgccag cagggttttg
     7861 aggtggcgca attcgataat ggaatccatg ggggcttcag acggcatatt gaaccggcgc
     7921 atattaaaat aattcatatg cgggtgcaaa tcggaaaaat gggcgtttcg ggcatttgcg
     7981 gttagaatgc ccgcttgttt ggaaatgaca ggaaaaaata tggttttgcg cagagatttt
     8041 ttagcctggt gcgacgaaac gttgcagacg gcatcgttta aagattacgc ccccaatggt
     8101 ttgcaggttg aagggaggga atatatcggg aaaatcgtta cgtcggtaac ggcaagcagg
     8161 gcagcgattg attttgctgt ggagcagaag gcagatttgc ttttggtaca tcacggtatg
     8221 ttctggaaaa acgagttgcc gaccgttact ggttggaaaa aagaacggat tgccgcactg
     8281 ttacggcacg acatcaatat ggcaggctac catctgcccc tggatgcaca tcccacactg
     8341 ggcaacaatg cccaactcgc cgacagattg ggttttgcga cagaaaaacg gttcggcgaa
     8401 caaaacctgc tcaactcggg cagcctgaaa caagccaaga cactcggcgc attggcggcg
     8461 catattgaaa cagttttgca acgtaagcct gtcgtcatcg gcaaacccga acgcgaaatc
     8521 cgacgggttg catggtgcag cggcggggcg caggggtttt ttcagacggc aatagacgaa
     8581 ggtgtcgatc tgtatttgac gggggaaatc tctgaagccc aataccacct tgccaatgaa
     8641 acgggtacgg ctttcatttc ggcagggcat cacgcgacgg aacgttacgg cgtacgcgcg
     8701 ctggcagaat cggcggcaga ggttttcggg ttggaagtgt gccattttga cgaaaacaat
     8761 ccggcttgag tcttgagaaa tatcataaaa ctttacctta ttttaaataa tgctttgagt
     8821 atcactgcaa actgggtaaa attgcaacgt ttaatggctc ggtattccca aaatattagg
     8881 acgactgaat aatggataat caagaaatca acaacggccg ccgccgtttc ctgacactcg
     8941 cgacctgcgg cgcgggcgga gtggcagcat tgggtgtggc aacgccgttt gtggccagtt
     9001 ttttcccttc ggaaaaagcc aaggccgccg gtgctgccgt cgaggtggat gtcagtaaaa
     9061 tcgaagcggg tcagctgctg accgccgagt ggcaaggcaa accgatttgg gtgctcaacc
     9121 gtacagatca gcagcttaaa gacctgaaag gcctgaacgg cgaacttacc gatcccaatt
     9181 ccgatgcgga acagcagccg gagtatgcta aaaacgagac ccgttcgatt aagccgaaca
     9241 tccttgtcgc catcggtatc tgcacccatt tgggctgctc gcccaccttc cgtcccgaca
     9301 ttgcccctgc cgatttgggt gcagactgga aaggcggctt cttctgcccc tgccacggtt
     9361 cgaaattcga cttggccggc cgcgtatata aaggtgttcc tgccccgacc aacctggttg
     9421 tcccgccata taaatacttg agcgacacaa ctatcttggt gggcgaagac taagaataag
     9481 gaacgataat tatggcaaac caaaccaata gcaaagcaaa agcattgtta ggctggatgg
     9541 acgctcgttt ccccctgtct aaaatgtgga atgagcattt ggctcaatac tacgcaccga
     9601 aaaactttaa tttttggtac tacttcggct ctttggccct gcttgtcctc gtgattcaaa
     9661 tcgtcagcgg cattttcctg accatgaact acaaacccga cggcaacctg aacgcctacc
     9721 atctgcctgc cgcctttacc gcagtagaat acattatgcg cgacgtgtcc ggcggttgga
     9781 ttatccgcta tatgcactcc accggcgcat cgttcttctt cattgttgtt tatctgcaca
     9841 tgttccgtgg tttgatttac ggttcgtaca aaaaaccccg tgagttggtg tggattttcg
     9901 gttctctgat tttcttggca ttgatggcgg aagcctttat gggctacctg ctgccttggg
     9961 gtcagatgtc cttttggggt gcgcaggtaa ttattaacct gttctccgcc atccctgtta
    10021 tcggtccgga tttgtccact tggattcgcg gcgacttcaa cgtttccgac gttaccctga
    10081 accgcttctt tgccctgcac gttatcgcgg tacctctggt attgctcggc ttggttgtgg
    10141 cgcacatcat cgccctgcac gaagtgggtt ctaacaaccc tgacggcgtt gaaatcaaga
    10201 aaaacaaaga cgaaaacggt atcccgcgcg acggtatccc gttccatcct tactataccg
    10261 ttaaagacat cttgggtgtg gttgtattcc tgattgtctt ctgtgccgta ttgttcttcg
    10321 caccggaagg cggcggctac ttcctcgaag cgccgaactt cgatgcggca aatgcgctga
    10381 aaacacctcc gcacattgcg ccggtatggt atttcacacc gttctacgcc attttgcgtg
    10441 cgattccctc gttcttggga acgcaggttt ggggtgtaat cggtatgggc gcggcggtcg
    10501 tgttgattgc cctgctgcct tggctggata ggggcgaggt gaaatccgtc cgttaccgcg
    10561 gcccgatttt caaaaccgca ttggtcttgt tcatcatttc ctttatcggt ttgggtattt
    10621 tgggcgcgat ggtggcaacc gatacgcgta ctttggtcgc gcgcatcctg tccttcgtct
    10681 atttcgcatt cttcctgggt atgccgttct ataccaagct ggataaaaac aaaccggttc
    10741 ccgaacgcgt aaccatgagc accgccaaac aaaaaatcat gttctttgtt tacgtcggca
    10801 ttaccgccgt gggtgcttac ctgtttgcaa ccaacatctg atgagggcag tcaaaatgaa
    10861 acaagctatg aaaaattggt ttgctgcctt gctgttggca gtacctatgg gtgcggcatt
    10921 tgcttcgggc ggtcatgcac actatgagaa agtcgatatc gatttgcgcg accaagtcag
    10981 cctgcagcgc ggcgcacaaa tctttaccaa ctattgcctg tcctgccact cggcaagcgg
    11041 tatgcgtttc aaccgtttga aagacatcgg tttgactgac gaagaaatca agaaaaacct
    11101 gatgtttacc accgataatg tcggcgatgt catgcattcg gcgatgaacc cgaaagatgc
    11161 ggcaaaatgg tttggtgctg ctccgcccga tttgacgttg attgcgcgtt ccaaaggtgc
    11221 agactacctt tacgcctata tgcgcggctt ctataaagat ccgacccgtc cgagcggctg
    11281 gaacaatact gtattcgata aagtcggtat gccccacccg ttgtgggaac agcaaggcgt
    11341 tcaagccgtt gagttggatg ccaaaggtca gccggttatg gtaaaagacg aacacggcga
    11401 gatgaagcct aagctgtatt gggaatctac cggtttgcac agccgccgtc tgcctaacgg
    11461 caaagtgatc caaaaagagt acgacgcata tgtacgcgat ttggtcaatt accttgtgta
    11521 catgggcgaa cctgcacaac tgcaacgcaa acgtataggc tatgtcgtga tgattttcct
    11581 atttgcggtt atgctgcctt tggcgtattt cctgaaaaaa gaatattgga aagacgtaca
    11641 ctaagcgttt ggaacaaaag ggcaaatcct ttagggtttg cccttttttc attttgcctg
    11701 ccgtttgaaa agcctgaatc cgtatgccgt ctgaacatag ctgcaacatt tcagacggca
    11761 tcattctaaa aatgtcagac atcgggaact taatcagggt ttgtggcaga ttgatattga
    11821 attaggaaaa ggtctgaaac cgcaagcagc atggtaaggc gatgtcgaac aggtttaaag
    11881 ccaatccgct atattttttg gagctatgtc gaacaaacag gtagaatcgg aataagcggc
    11941 ggttttgtcc ggtgtgaaaa tgtttgaata ttaggatgat tgatggaact gatgactgtt
    12001 ttgctgcctt tggcggcgtt ggtgtcgggc gtgttgttta catggttgct gatgaagggc
    12061 aggcttcagg gcgagtttgc cgatttgaac gcgcaactgg cggaaaaggc ggcaagatgt
    12121 gattttgtcg aacaggcaca cggcaaaacc gtgtcggaat tggcggtgtt ggacgggaaa
    12181 taccggcatt tgcaggacga aaattatgct ttgggcaacc gtttttccgc agccgaaaag
    12241 cagattgccc atttgcagga aaaagaggcg gagtcggcgc ggctgaagca gtcgtatatc
    12301 gagttgcagg aaaaggcaca gggtttggcg gttgaaaacg aacgtttggc aacgcagctc
    12361 ggacaggaac ggaaggcgtt tgccgaccaa tatgccttgg aacgccaaat ccgccaaaga
    12421 atcgaaaccg atttggaaga aagccgccaa actgtccgcg acgtgcaaaa cgacctttcc
    12481 gatgtgggca accgttttgc cgcagccgaa aaacagattg cccatttgca ggaaaaagag
    12541 gcggaagcgg agcggttgag gcagtcgcat accgagttgc aggaaaaggc acagggtttg
    12601 gcggttgaaa acgaacgttt ggcaacgcaa atcgaacagg aacgccttgc ttctgaagag
    12661 aagctgtcct tactgggcga ggcgcgcaag agtttgagcg atcagtttca aaatcttgcc
    12721 aacacgattt tggaagaaaa aagccgccgt tttaccgagc agaaccgcga gcagctccat
    12781 caggttttga acccgctaaa cgaacgcatc cacggtttcg gcgagttggt caagcaaacc
    12841 tatgataaag aatcgcgcga gcggctgacg ttggaaaacg aattgaaaag gcttcagggg
    12901 ttgaacgcgc agctgcacag cgaggcaaag gccctgacca acgcgctgac cggtacgcag
    12961 aataaagttc agggcaattg gggcgagatg attctggaaa cggttttgga aaattccggc
    13021 cttcagaaag ggcgggaata tgtggttcag gcggcatccg tccgaaaaga ggaagacggc
    13081 ggcacgcgcc gcctccagcc cgacgttttg gtcaacctgc ccgacaacaa gcagattgtg
    13141 attgattcca aggtttcgct gacggcttat gtgcgctaca cgcaggcggc ggatgcggat
    13201 acggcggcac gcgaactggc ggcacacgtt gccagcatcc gtgcacacat gaaaggcttg
    13261 tcgctgaagg attacaccga tttggaaggt gtgaacacat tggatttcgt ctttatgttt
    13321 atccctgtcg aaccggccta cctgttggcg ttgcagaatg acgcgggctt gttccaagag
    13381 tgtttcgaca aacggattat gctggtcggc cccagtacgc tgctggcgac tttgcggacg
    13441 gtggcgaata tttggcgcaa cgagcagcaa aaccaaaacg cgctggcgat tgcggacgaa
    13501 ggcggcaagc tgtacgacaa atttgtcggc tttgtacaga cgctcgaaag cgtcggcaaa
    13561 ggcatcgatc aggcgcaaaa cagctttcag acggcattca agcaacttgc cgaagggcgc
    13621 gggaatctgg tcggccgcgc cgagaaactg cgtctgttgg gtgtgaaggc aaacaagcag
    13681 cttgcacgcg atttgaccga acgtgccaat gaaacaacgg cgttgtcgga atctttggaa
    13741 tatgtggcag aagatgaagc agtctgactt gtgcggaaaa gcttgtttca gcaggggcgg
    13801 gaatgccgaa agcgcggcgt aactgtaccg gttctgattt ggggtttgtt tgaagtactt
    13861 accaaagcct tgtccggcga ggtacaccgg caaggcggcg gttgtctgag ccttttgcgt
    13921 ttcagacggc atcagtgcag atgatgccgt ctgaagaccg tagggaaggc ggttagaaaa
    13981 acggattgtg ccgcttttcg tgtccgatgg aagtcatacg cccgtgtccg gcgacaactt
    14041 gcacggtttc gggaagggtg aataatttgt tgcggatatt attgattaag tcggcgtggt
    14101 tgccgcgcgg aaaatcggtt ctgcctatgg tttcgtaaaa cagcacgtcg cccgcaatca
    14161 gcaattccgc ctcggcacaa taaaagacga tatgtcccgg cgtatggccc ggaatatgca
    14221 gcacttgaaa ggcatagcgt ccgaccgtga gcgtttcgcc ttcttcgagc caacggttcg
    14281 gcgcaaaggc gggcgagacg ggaaatccgt attgcgcggt ggtttgcggc agcgattgga
    14341 gcaggaattc atcgtccgga tgcggcccga ggacagggac tttatgcgtt ttcaacattt
    14401 cgaccacgcc gcccgcgtga tcgagatggc cgtgcgtcag ccagattgcc gtgagcgtaa
    14461 gtttgcggtt tgccaacgct tgcagcagga acggcacatc gccgccgaca tcggtcagga
    14521 cggcttcgcc actttcgtcg tcccaaatca gggtgcagtt ttggcggaag ggggtaacgg
    14581 ggaagatttc gtaacgtagg gtcatcggcg tgttcctaaa cggtttttca gacggcatcg
    14641 ggtttgccgt ttgttttatg gcggtttgcc gcctgtttgg atattggggg aatcgggtga
    14701 ttaagaagac aatcggcggc atcataccga tttttatggc ggttttcatc cctgcatcgg
    14761 caggcgcggc ggatttgatg ctggcgcagg aatacaaagg gcaggatatt gccggctggg
    14821 cgatgagcga gaaactcgac ggcgtgcgcg cgtattggga cggaaagcac ctgataagcc
    14881 gtcagggcta tgcgtttacg ccccccaaag gttttaccgc tcagtttccg ccttatcctt
    14941 tggacggcga attgtatagc ggacgcggtc agttcgagca gatttccgcc gccgtgcgtt
    15001 ctgcttcttc agattggcgc ggcatccgcc tgcacgtttt cgatgtaccc aaggcgcagg
    15061 gcaacctcta ccggcgtttg gcagtcgcaa cgcaatggct gaaaacgcat ccgaacgcgc
    15121 cgattaccat catcccgcaa atcaaagtgc gcgaccggca gcacgcgatg gactttttaa
    15181 aacaaatcga agcgcagggc ggcgaaggcg tgatgctgcg tcagcccgaa tcccgttaca
    15241 gcggcggcag gagcagccaa ttattgaagc tgaaaagcca atacgacgac gaatgcacgg
    15301 taacgcggca ctatgagggc aaagggcgaa aagccggacg gttgggcgcg gtcggctgca
    15361 aaaaccggca cggcgaattc cgcatcggca gcggtttcaa agacaaagac cgcgacaacc
    15421 cgcccaaaat cggcacgctg attacctacc gctaccgtgg ctttacgcag aaaggtacgc
    15481 cgaaatttgc cacatttgtg cgcgtgcgta ccgaccgctg agcaggacgg ttcggacggc
    15541 agccgatact ttggtttata attttccttt taccgaccga ttccgacata tgaccgattt
    15601 agaaaccaaa cgccttgaaa cacaggcgat gcttgaaaac gccgatcttt tgttcgacca
    15661 aggccaatgc cgtgccgcac tgcaaaaagt ggcggacgag attacgcgcg atttgggcgg
    15721 caaatatccg ctgctgctgc ccgtgatggg cggcgcggtg gtgtttacgg ggcagttgct
    15781 gccgctgttg cgttttccct tagattttga ttatgttcac gtttcccgtt acggcgacaa
    15841 gctggagggc ggcgcgttca actggaagcg tatgcccgat gcggaacaaa tccggggcag
    15901 gcacgtcgtc gtgctggacg atattttgga cgaagggcat acgatgtccg ccattcaagc
    15961 caaacttttg gaaatgggtg cggcaagctg ccgtgcggcg gtgttcgcca acaagctgat
    16021 cgacaaaccc aagcctatcc gagccgatta tgtcggactg gatgtgccga accgttatgt
    16081 tttcggttac ggcatggatg cggcgggctg ctggcgcaat ctgggcgaga tttacgcatt
    16141 ggacggaaaa taaggacgcg atgccgtctg aaggcctttc agacggcatc gcgcccatac
    16201 gccggtagga caacgaggaa caggacgcat aatatgatag ggcttttaat catcacacac
    16261 gaaaccatag gcgaagccta ccgcaagctg gcacatcatt tttttccggg cggactgcct
    16321 gaaaacgtcc gcatactcgg cgtgcaaccg acggaagacc aagacgacat catcaacaac
    16381 gccattgccg cgcttcagga gtttcccgac aacgacggcg tgctgattat gaccgatatt
    16441 ttcggcgcga ccccctgcaa tgcagcccgc cgcctcgtgc gcgaaaacaa atcggcgatt
    16501 ttgaccgggc tgaacgcgcc gatgatggtt aaggccgtcc aatattcgcc ggcggcggaa
    16561 gaccttgccg cctttaccga atgcgtcagg gaggcggcgg taaaaggcat tttcgccatc
    16621 acgtccgcgc ccgaagattt ggtgtgccgg cgcagcggcg atgccgtctg aagaagcggc
    16681 agggcaggaa aacattttaa acggcgtctg ctgccgatat acataacacg ggaatcgaaa
    16741 tgctcaaaca atccatcgaa atcatcaaca aactcggact tcacgcccgt gcctccagca
    16801 aattcaccca aaccgcgtct cagtttcaaa gcgaagtctg ggtaacgaaa aacggcagcc
    16861 gcgtcaacgg caaaagcatc atggggctga tgatgctcgc cgccgccaag ggtacggtca
    16921 tcgaactgga gacggacggc gcggacgagg cggaagcgat gcgcgccctg accgacttaa
    16981 tcaacggcta cttcggcgag ggcgaataat gagtatcgtg ctgcacggcg tggcggcggg
    17041 caaaggcatt gccgtcggtt gcgcccacct gattgcgcgc ggtacggagg aagtgccgca
    17101 gtatgatgtt gcggaggcgg acaccgatgc cgaagccgaa cgtttcgatg ccgccgtcaa
    17161 agccacgcgc aaagagttgg aacagctccg cagcgcaatt cccgaaaacg ccccgaccga
    17221 gttgggcgcg ttcatctcgc tgcacctgat gctcttgacc gatgttacct tgtcgcgcga
    17281 acccgtcgat attttaaggg aacaaaaaat caacgccgag tgggcattga agcagcagag
    17341 cgacaaactc gccgcccaat tcgacaatat ggacgatgcc tatttgcgcg aacgcaagca
    17401 ggatatgctg caagtcgtcc gccgcatcca caacaacctg atcgggcagg gcaacgagtt
    17461 ggaagttgcc gacaacctgt ttgacgaaac cgttctgatt gcaaacgacc tttcgcccgc
    17521 cgacacggtt ttgtttaaag agcagcgcat tgccgccttc gttaccgatg ccggcggccc
    17581 caccgggcat acggcgattt tgggcaggag cttggacatc ccgtccgtcg tcgggctgca
    17641 caacgcgcgc aaactgatta ccgaaggcga aacggtcatt gtggacggta tcaacggcgt
    17701 gttgattatc gcgccggatg agtcggtgtt gaacgaatac cgccgccgtg cccgcgaata
    17761 ccgcagccac aaacgcgatt tgaacaagct caaaaaaacc gccgccgcca ccgccgacgg
    17821 ggtctgcatc gagcttgtgg gcaatataga atccgccgaa gacgtgaaac cgctgcacaa
    17881 cctcggcgca gacggcatcg ggctgttccg cagcgagttt ctttacctga accgcgatac
    17941 gatgccgtct gaagacgagc agtacgaagt gtacagcgcg attgtcaaaa aaatgaaggg
    18001 caaaagcgtg accatccgca ccgttgattt gggcgtggat aaaaaccccc gctggttcgg
    18061 gaaaaacagc acgcccaacg gcagcctcaa ccccgcgctg ggcatgaccg gcatccgcct
    18121 gtgccttgcc gaaccggtca tgttccgcac ccagatgcgc gccatcctcc gtgcggcggt
    18181 acacggccct gtgcggatga tgtggccgat gattacctcc gtatccgaag tgcgccagtg
    18241 cctgattcat ttggacaccg cccgccgcca gcttgccgaa cgcggcgatg ccttcggcga
    18301 tgtcggcatc ggctgcatga ttgaaattcc gtctgccgcg ctgaccgtcg gcagtatttt
    18361 gaaactggtc gatttcatct ccgtcggtac caacgacctg attcaataca tcttgtccgt
    18421 cgatcgcggc gacgacagcg tcagccatct ttaccagccc ggacatcccg ccgtgctgaa
    18481 aatgctgcaa cacgtcatcc gcaccgccaa ccgcatggac aaagacgtgt ccgtatgcgg
    18541 cgagatggcg ggcgataccg cgtttacccg cgttttattg ggtatggggc tgcgccgttt
    18601 ttccatgaac cccaacaaca tcctgcccgt caaaaacatc attctgcaca gcaatgtcgg
    18661 gcagctcgaa agcgatattg tgaaagtcat ccgctgcgaa gacgaagaga agtcggaaaa
    18721 gctgatcaaa cagatcaaca gcgtgtctgt cgaggaagaa gccgacttca aagggcggaa
    18781 ataaatacgg caggtgaaaa atagaaatac ttaacaatgc ccgcaatctg aaattttgcc
    18841 attcttgcaa aatagaaaac cgaaacagaa acccaaaatc ggccattccc tcaaaaacag
    18901 aaaaccaaaa tcagaaacct aaaatccgtc attcccgcgc aggcgggaat ctaggtctgt
    18961 cggcacggaa acttgtcggg taaa
//
LOCUS       DAWHRS010000021        18716 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWHRS010000021 DAWHRS010000000
VERSION     DAWHRS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.021"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(72..1289)
                     /gene="nqrF"
                     /locus_tag="WIB82_000515"
     CDS             complement(72..1289)
                     /gene="nqrF"
                     /locus_tag="WIB82_000515"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ3841861.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNNEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLVAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(1303..1896)
                     /gene="nqrE"
                     /locus_tag="WIB82_000516"
     CDS             complement(1303..1896)
                     /gene="nqrE"
                     /locus_tag="WIB82_000516"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ3841862.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(1900..2526)
                     /locus_tag="WIB82_000517"
     CDS             complement(1900..2526)
                     /locus_tag="WIB82_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ3841863.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(2526..3302)
                     /locus_tag="WIB82_000518"
     CDS             complement(2526..3302)
                     /locus_tag="WIB82_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ3841864.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(3295..4527)
                     /locus_tag="WIB82_000519"
     CDS             complement(3295..4527)
                     /locus_tag="WIB82_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ3841865.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVSGKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLSLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(4530..5873)
                     /locus_tag="WIB82_000520"
     CDS             complement(4530..5873)
                     /locus_tag="WIB82_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ3841866.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(6221..7642)
                     /locus_tag="WIB82_000521"
     CDS             complement(6221..7642)
                     /locus_tag="WIB82_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ3841867.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(8001..8360)
                     /locus_tag="WIB82_000522"
     CDS             complement(8001..8360)
                     /locus_tag="WIB82_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ3841868.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(8357..8962)
                     /locus_tag="WIB82_000523"
     CDS             complement(8357..8962)
                     /locus_tag="WIB82_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ3841869.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(9009..9491)
                     /locus_tag="WIB82_000524"
     CDS             complement(9009..9491)
                     /locus_tag="WIB82_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ3841870.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      9694..9791
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10002..11102
                     /gene="gcvT"
                     /locus_tag="WIB82_000525"
     CDS             10002..11102
                     /gene="gcvT"
                     /locus_tag="WIB82_000525"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ3841871.1"
                     /translation="MTALKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            11264..11650
                     /gene="gcvH"
                     /locus_tag="WIB82_000526"
     CDS             11264..11650
                     /gene="gcvH"
                     /locus_tag="WIB82_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ3841872.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDSLLTAEQYAGEVD"
     gene            11802..13049
                     /locus_tag="WIB82_000527"
     CDS             11802..13049
                     /locus_tag="WIB82_000527"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ3841873.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            13325..13750
                     /locus_tag="WIB82_000528"
                     /pseudo
     CDS             13325..13750
                     /locus_tag="WIB82_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            13756..14985
                     /locus_tag="WIB82_000529"
                     /pseudo
     CDS             13756..14985
                     /locus_tag="WIB82_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            15043..15663
                     /locus_tag="WIB82_000530"
     CDS             15043..15663
                     /locus_tag="WIB82_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3841874.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLIGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            15674..15805
                     /locus_tag="WIB82_000531"
                     /pseudo
     CDS             15674..15805
                     /locus_tag="WIB82_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            15802..16296
                     /locus_tag="WIB82_000532"
     CDS             15802..16296
                     /locus_tag="WIB82_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ3841875.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(16384..18045)
                     /locus_tag="WIB82_000533"
     CDS             complement(16384..18045)
                     /locus_tag="WIB82_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ3841876.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(18175..>18716)
                     /locus_tag="WIB82_000534"
     CDS             complement(18175..>18716)
                     /locus_tag="WIB82_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ3841877.1"
                     /translation="QSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVLK
                     KLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVLR
                     GIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFTR
                     SPKRQTEFAVGQVKWWRAY"
BASE COUNT         4586 a         4798 c         5029 g         4303 t
ORIGIN      
        1 gtgttcggac ggtatcggat aaaaaaagga agaaaccgag gtttcttcct tttgtgtttg
       61 aagccgaata tttaaccgcc gaaatcgtcc aagaggatgt tttcgtcttc cacgcccaag
      121 tctttgagca ttttgatgac ggactggttc atgattggag gaccacacat ataaaattcg
      181 cagtcttccg gtgcttcgtg atttttcagg tggttttcgt aaaccacgtt gtgaatgaag
      241 cctgtgtagc cgtcccagtt gtcttcgggc aatgggtcgg acagggcgac gtgccacgta
      301 aagttcggga actctgccac gagttggtcg aagtcttcga catagaacat ctcgcgtttg
      361 gaacgcgcac cataccagaa ggtgattttg cgtttggagt tcaaacgttt caactggtcg
      421 aaaatgtggg aacgcatcgg cgccataccc gcaccgccgc cgataaatac catttcggca
      481 tcggtgtctt tggcgaagaa ttcgccgaac gggccggaaa tcgtaacttt gtcgccgggt
      541 ttgagcgacc agatgtaaga agacatttgt cccggaggcg catcgggtac gcgcggaggc
      601 ggcgtggcga tacgcacgtt cagcataatg atgccttttt cttctggata tgaagccata
      661 gagtaggcgc gcaaaatcgg ctcgtccact ttggaaacgt attgccacaa attgtatttg
      721 tcccagtctt cgtgatattc cttaggaatg tcgaagtctt tgtaggcaac agtgtgagga
      781 ggagcttcaa tttgaatgta gccgccggcg cggaagggga cttcttcgcc ttcgggaatg
      841 gcaagcttga gttctttaat gaacgtggct ttgttatcgt tggagatgac ggtgcattcc
      901 cattttttca cgccgaacac ttcttcgggg acttcgatgt ccatgtcggt tttgacgttg
      961 acttggcacg acagacggca gccttcgcgt gcttcgcgtt tgctgatgtg ggacagctcg
     1021 gtcggcagga tgtcgccgcc gccgcttttt acgacgacgc ggcattgtcc gcacgaaccg
     1081 cccccgccgc aggcggaggg gataaagatg ccttcgttgg caagcgcgcc caagagtttg
     1141 ccgccggcgg gcatcgtcag ctctttttcg ttattgactt tgatggtgat gtcgccttcg
     1201 ctgaccagtt tggatttggc aaacagaatc atcagtgcca aaaccaaaac gatgacggta
     1261 aacatcacga tacctaaaat aatctccata ccgatccctt tcttataact ggatgccgga
     1321 gaacgacata aacgccatcg ccatcaggcc ggcggcgata aaggtaatgc ccaagccttt
     1381 gaggcctttg ggagcgtccg aatatttcat tttttcggta atgcccgcca aagcgacaat
     1441 cgccaacatc cagcccaagc ccgcgccgaa accgtataca acggactcgc cgaagttgta
     1501 ttcgcgttgc gccataaacg atacggcgcc gaaaatcgcg cagttcacgg taatcagcgg
     1561 caggtagatg cccaacgcgt tatagagggc ggggacgaat ttatccaaga acatttccaa
     1621 aatctgcacc aaagcggcaa tcacgccgat gaaggtgatg aatttcaaaa aggtcaaatc
     1681 cacgccttcg acaatcgcgc cgtctttgag cagcgagtaa acgagttggt tgacagggac
     1741 ggacagcccg agtacgaaaa ttaccgccac acccaaaccg aatgcggtgg atactttttt
     1801 ggataccgcc aaaaacgtgc acatacccaa aaagaaggat agtgccatat tttcaatgaa
     1861 gacggatttg atgaagaggc tcaaatagtg ttccatagct tattcctccg cctgttcggg
     1921 tttccaggta cgcagtcccc aaatcaaaaa gccgatgatg aagaacgcgc tgggggcgag
     1981 caggaacaag ccgttggtct gataccagcc gccgtcctgc acggtttgga aaacggtgta
     2041 gcccaagagt ttgcccgagc cgatcagttc gcggacggtg gcgacgacaa gcagcatcat
     2101 cccgtagccc gcgccgttgc cgatgccgtc gatcaggctt tccagcggcg gctctttcat
     2161 cgcaaatgct tcggcgcggc ccatcacgat acagttggta ataatcagac cgacgaatac
     2221 ggaaagctgt ttggacaatt cgtaggcaaa tgcctgcaag agttggtcga ccagcgtaac
     2281 cagcgacgcg ataatcgcca tttgcacgat gatgcggatg ctgttgggga tgtagttgcg
     2341 taccagcgaa atgaagaagc tggaaaaacc ggttaccaaa gctacggaaa tacccatcac
     2401 gatggccgtc tgaagtttgg tggtaaccgc caaagccgaa caaataccca aaacctgcaa
     2461 ggcaatcggg ttgttgtcga taaagggtga aaacatcaaa tgtttcaagc gtttcatatc
     2521 agccattatt gtgctcctgc tgatttcaat ttgttcaggt aggggatata gccgttttcg
     2581 ccgaaccagt aggcgaacga accttgcacg cctttggaag tcagcgacgc gccggagagg
     2641 gcatctacgc cgtgttcttt gtccgaaccc gcgcctttgc cgacgtgcag ggcgagtttg
     2701 ccttgtccgt caaacagttt tttgccgacg aatttttgct gccacaaagg gttgccgatt
     2761 tcgccgccca agcccggggt ttcgccttgc tcgtaatagg taatgccgtt gatggtgttg
     2821 ccgtcgggtt ggatggcgac aaaaccgtac atcaccgacc acaaaccgtt gccgtgcata
     2881 ggcaggatga tttgcccgat tttgccgtct tcacctttta ccaaataaac ctcagtgtat
     2941 ttggcacggc ttttgatgcc tgccaaatcg tcttcgggtt tgatgcggat gctttgggca
     3001 gggtctttgc ctgcgatgcg cgcgctgaaa tctttaggcg catcggcgac gtattcgccg
     3061 gtcgccaaat cgacaacacg ttgctcgata cgctcggcaa aggttttacc gatgtcggtg
     3121 tccttatcca tcaaaccggc tacgctcaag atatagcctt gtttgtcttg gagtttttgt
     3181 ttctcttgga tgggtttcaa gccaacgacc gcaccggcaa cgatgaccga gcaaatcagg
     3241 ctgaccgcca acacgacaat cagcgtgccg ctgaagctgt ctttatcgaa tttcttagcc
     3301 attgctgcgc gcctttctgc gtttgatgtt cgcttgtgcg acgaaatagt cgaaaatcgg
     3361 ggcaaacagg ttggcaaaca gaatcgccaa catcatgcct tcggggtaag ccggattgac
     3421 cacgcggatc aatacgcaca tcacaccgat tagcgcgccg taccaccatt tgccgacatt
     3481 ggtaaaggaa gcggaaacag ggtcggttgc cataaacagc ataccgatgg cgaagccgcc
     3541 gacgaccaaa tgccagtacc aaggcatagc aaacatggcg ttggtgtccg aaccgatgaa
     3601 gttgaacagc gaagacatgg caatcatacc gatcatcacg ccggcaataa tgcgccaaga
     3661 agcgatgcgg gcaaacacga taaacgcgcc gccgattaag agtgccaaag tggagacttc
     3721 gccgatggag ccgggcagtt tgccgataaa cgcatccatc caagtgatgg tttgaccggt
     3781 tacggcgttt ttcaggccgt ctgcaccgtg tgccgcccat tgcgccagcg cggttgcgcc
     3841 ggaatagccg tcaaccgccg tccaaaccgc atcgccgctc aagttggcag ggtaggcgaa
     3901 gaacaggaag gcacggcctg ccagcgcagg gttcatgaag tttttacctg taccgccgaa
     3961 tacttctttc gcaaccacaa cgccgaaaga aatacccaaa gctgcctgcc acagcgacag
     4021 cgtgggcgga acgattaagg caaacagaat cgaggtaaca aagaaacctt cgttgatttc
     4081 atgtttgcgc acggtggcga acaaaacttc ccagaaaccg cccacgacaa atacggtcgc
     4141 gtaaatcggc agaaagtaaa tcgcgccgaa cagcattttg cccgacacac ccgcttcaga
     4201 cgacatattg atgcccaaag cgtcggcaag ggtgtaatgc cagtcgttgg cgatgctttg
     4261 ttgcagcaaa tcgggcgtta aggcaccgaa tgcctgcgcg ccgacgttgt acataccgta
     4321 gaacatggca gggaacaaag ccagccacac caaaatcatc atgcgcttgg agtcgagcgc
     4381 gtcgcggacg tgcgccgctt tgcgcgttac cgcgccggat gtgtagaaaa ttgtcgccgc
     4441 cgcttcgtag agggcatacc atttttcatg tttgccgccc ggcaggaagt gcggttcgat
     4501 tttttccaga aaatgtttca agcccataat cagccttcct tctcaatggt ttccagcacc
     4561 ttacgcaaca gcgggccgta ttcgtatttg cccgggcaga cgaagctgca caaagcgagg
     4621 tcttcttcgt ccaattccaa gcaacccaat gcctgcgcgc tgtcggtatc gccgacgatt
     4681 aaatcgcgca aaagcagggt gggcaggata tccaagggca tcacgcgctc gtaagtgccg
     4741 atgggaacca tagcgcggtc gccgccgttg acggctgtcg tgaacttgaa gagtttgttt
     4801 ttcaggaaat ggccgagggt cgtccgcgtg atggagtatt tgtccggctg cggcgcaacc
     4861 cagccgaaca gctctttgct gcggccttct tcgataacgg aaatctgatt gtggtagcgt
     4921 cccaaataat catgcgcgcc ttgtgcaatc gcgccgttca ataccgaacc ggaaatcacg
     4981 cggttgtctg cgtcaaccaa ttcgcccgca gtaatttgcg atactttcgc acccaaaacg
     5041 gtacgcaaga ggcgtggttt gttgacttga gaaccaccca aggcaaccac gcgctcggtg
     5101 ttcagacggc ctgttgcaaa caaacgtccg atggcaatta catcttgata attgatggtc
     5161 caaacggttt tgttcgcacc gacaggctca atgaaatgaa tgtgcgtgcc actcaaaccg
     5221 gcaggatgcg ggccgccaaa ttcatgagtt tcgatgttgg cggcgttttc agacggcacg
     5281 tctgcgccgg ctgccttaca aacatggatt ttgcgttcgg tcaaacggct caacaccaac
     5341 aggccgcgtc tgaaatcctc ggcggcttct ttgataatga ccgtagggtc ggcagccagc
     5401 ggattggtgt ccatcgcatt gacgaagatg gcgaacggct cggcatcgac ggcagggatt
     5461 ttgctgaacg gacgggtacg cagcgcagtc cacaaaccgg attggatcag attgcggcgc
     5521 acttcttcac cgcttaagtt tgccaacgct tcgggcgcgt agcgttcgaa ctcgatttcg
     5581 tcgttgcctt caacggcaat cacgaccgac tgaagtacgc gcttttcgcc gcgatggatg
     5641 gcggcgattt tgcctgaaac cggcgcggta aacaccacgc ccggattctt tttgtcttca
     5701 aacagcactt ggcctttttt gacggcatcg ccttccttga ctttcatcga ggggcgcata
     5761 ccggcatatt cttcgccaag caacgcgact tcggtaatga cgggcccgtc ataaatgact
     5821 tgctccggtc tgcccgcgat gggcaggttt agaccttttt tgattttaat catatatttg
     5881 cattacttgt gatggttaag gtaaaaacgg cgtgttttga taccgtgtcg cgtggcatca
     5941 aaagcattga ataaattaat gtagcaaagt gttagattct atcaggaatt gtacctgttt
     6001 gtcagatttg ctgctttttt ccttgcggaa gccgttttta tagtggatta aatttaaacc
     6061 agtacggcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt
     6121 cctgattttt gttaatccac tataattgtc ggaagggggg atattgattt gattatgccg
     6181 gaatttaaaa tgccgtctga atgttcagac ggcatagcgt ttacagcagt ttgaaaacga
     6241 aaaagataag ggtatgtacg atgaagacgg gtgtcaggaa ggcgaccgac cacatcatat
     6301 agccgaagaa agtcggcatc ggtacgccgc gctgttcggc aatggccttg accatgaagt
     6361 tcggtgcgtt gccgatgtag gtcagtgcgc ccatgaatac cgaacccata gaaaccgcca
     6421 gcagcgaatg aaacagggaa cccgtcatca aggcttgggc atcgccgcct gccatattga
     6481 aaaaaacgag ataagtgggc gcgttatcca agaatgccga caatatgccg ctcatccaaa
     6541 aatacatcac attaatcgga tgacctgccg tatcgtgaac cagcgatacc accccgccca
     6601 gcgcgcctgc ctcgcctgct ttcagaatgc tcaggacggg aaagatggtg atgaagatgc
     6661 cgaggaagag tttgcccact tcggcgatgg gttcaaagtt gaattcgttg cctgcgcgga
     6721 cttgtttggg cgtgattgcc atagatacgg cggtcaatgc aatcaggatg acatcgcgga
     6781 cgaggttttg cagggcgtaa cggctgccga ggatttcaaa tcccgggtgt tcgggtttcc
     6841 aaaggccgga cattagaacc gcgccgacca cgcccgaaag caggaggaag ttccatttgc
     6901 cgaagatggc gattttttcg ggtttttcct gttgtgccgg cgtatcttgt gcaatgcttt
     6961 cctgtttgaa gaaacggttg tcgatgaaat agaaggcggt caacaggaca gcggtgctga
     7021 tcaggacggg ggcgaacata tgtttgaccg tccacatgaa atctacgcct ttgaggaagc
     7081 cgaggaagag tggggggtcg cccaaagggg tcagaccgcc gccgatgttt gcaaccagga
     7141 aaatgaagaa gatgacgatg tgcacgcggc gggtacggtt ttggttggct ttcagcagcg
     7201 gacgaatcat cagcattgct gcgccggtcg ttcccatgat agaggcaagt gccgtaccga
     7261 cggcaagcag ggcggtgttg agcttgggtg tgccgttcaa gtcgccccaa accaaaatgc
     7321 cgcctgaaat ggtgtacagg gcaagcagca gcaggatgaa agggatgtat tcttcaacga
     7381 gtgcgtgtgc gacggtatgg ataccggcgg acgcgccaaa aaccaaactg aacgggatga
     7441 ggaagagcaa tgtccaaaag gcggtaattt tgccgtaatg gtgatgccag gtatgcgaaa
     7501 aaaacaaggg acccaatgcg atagacagca aaatcagggc aaagggcagg ctccacagca
     7561 ggtttaggtt tgcgccgtcc aaatctgcgg cgtaaaccga tgcggggaaa agcattagtg
     7621 aaaacagggg taggtggcgc atcgtgtttc ctcgattcaa gcactgcctt gcgcggcgcg
     7681 cgggagtgat acaggcaccg tgccgcccgg acataggcgg actgtgctgc ctgtttgttt
     7741 ttgaaaaaag ttttccgatt gattgtaaag taaataatca ggataaacca gtttcccaaa
     7801 ccggaaggcg gcgggaaggc ggattgccgt gcttgggaat atatgttctt tttgataaat
     7861 aattttattt aaaacaaata aatatatgtc tttaataaat aatctatcga aaacgaaaaa
     7921 tgaatttatt ttaacatata tttgcaatga aacaggtttg ccccacccgt ttgtttgccc
     7981 ttatcccttt cagacggcat tcaagattcg ggcctgcgcc acatccatat ggcgacaagg
     8041 gaacaaaaaa ccgatgaaac cgccccgacc caccagcgtt ggggaaactg ccaaaacatt
     8101 atcaggcagg atgcggtcat catactgatg gcgaatattt tggctttgcg aggcactgcg
     8161 ccgttttgtt cccagttatg aaccatcgga ccgaaatagc ggtgccggtg cagccagcgg
     8221 tgaaagcgcg gggatgcctt tgcccagcag gcggcggaga gcagtacgaa cggcgtggtc
     8281 ggcaacagcg gcaaaaaaat gccgattatc cccaacagca gggagatgcc gccgcaggca
     8341 attaaaagat aacgtatcat tttgaaatat ttttcttatt gtgcggataa gggcaggatg
     8401 tgataccgag ttttgcccag ccttcatgtc ccattttttc cagcagggcg atattgcgtt
     8461 cgaatattgc cgaagcgtcg ggaaaggctt gtgcggcttt ggcaatgctg tcttcgcgga
     8521 tgaggtgcag cgtcggatag ggagaacggt tggtgtagtt gccgatgtcg tcgggttcgg
     8581 tattctgaaa gcggaattgg ggatggaacg gtgcgatttg gacgatgcct tccaagccgt
     8641 tttcgataac ggcggcatcg gcaatgtcga gcatatcgtt gaatacgtcg aaatcgggga
     8701 atagggtcgg gtgaaccagc agggtggttt ccagttcggt ggcgggtgta ttgcccagtc
     8761 gctgcagttc ttcgtccaag tcttccaaaa aactgtcaag gtgtttggct tcgctgattg
     8821 cgatgcggac aaggttttta acgtgggggg ctttggcaaa gggacacagg ttcagaccga
     8881 tgacggcttt ttccaaccat tgtccggtgt gttcggcaac agcatcttta ttttcggaag
     8941 tattgatatt cattattgtc atgtaaatgt gtttgcagat tgcacgtgcg ggaaaatcgg
     9001 gaagggcact attccttcag caggtggttg agcggcaggg aggtggtgtg tttgatttct
     9061 tttaaaacaa agctcgattg cgcatcttgt acgccgtggt gggacaggag cgtatccaaa
     9121 acaaaatggg aaaacgcgtt catatcggta aaaaacgcct gaagcaggta gtcggtttcc
     9181 cctgtcaggg cgaagcagct caagacttcc ggccattttc ggaccgatgc ggcaaagtct
     9241 tcccgcgcgt cttttgcttt gcggatggaa acgcggataa atgcctgaag ccccaggttg
     9301 acggattccg gagacagcag cgcggcatat tggcggacga tgccggcatc ttccaactgc
     9361 ttcagacggc gcaggcacgg agaaggcgaa agtgcgacac gttcggacag ttcgacattg
     9421 gtcagcctgc cgttttcctg gagaacctgt aagattttaa tatcggtttt gtctaaagtg
     9481 agttggggca tatttgcgtt ccgttttaag gaattcggat tgtctgtccg tatgtttgcg
     9541 gcaatccgca cagatggaga ccatattaac atataaaaag ttataccgtc atccgggaca
     9601 aattttgttt tcggaaaatc atgtgaaaac agaggcggtc ggtttgcatc tctttaagac
     9661 ggcttgccca aaccgccgat tcaagacata atcgggaaat gtgcaggaga gtgttacacc
     9721 caactacaat gtaaccaccg aaggcgcaga cacccttaaa tcgctcaggt atcagggact
     9781 gcacattgaa acaaacaatc tggagagcgg cgttggaata acgtccaccg aaggggagaa
     9841 ggccgtctga accaccattc agacaaccgc gcaaagcagt gagcagactg gtttgccatc
     9901 atgcggatac agccgaaaat ctcaggttca aggacagata gggtcatccg cgcacaggtg
     9961 cgcgggcggc atctgaacaa aaaatccgga gaaacttgag aatgactgct ctgaaaacca
    10021 ccccatttca tcaagcccat caagatgcag gcgcgaagct ggtcgatttt gccggctggg
    10081 agctgcccat ccattatggt tcacaaatcg ccgaacacga agccgtgcgc accgacgccg
    10141 gtatgtttga cgtatcccat atgctcgtta ccgacgtagc aggcgcaaat gccaaagcct
    10201 ttttccgcaa attgattgcc aacgatgtcg ccaagctcgc ttttgtcggc aaagcccttt
    10261 attccgcttt gctcaacgac aacggcggtg tgattgacga cttaatcgtt taccgcacca
    10321 atgaagccga aacccaatac cgcatcgtgt ccaacggcgc gacccgcgaa aaagacacgg
    10381 cgcaattcca caaagtcgga caagagttcg gcgtcgcctt caatccgcgc tacgacctcg
    10441 gcatgctcgc cgtacaaggc cctaaagcca ttgaaaaact cctgaccgtc aaacccgaat
    10501 gggcagatgt cgtccataac ctcaaaccgt tccaaggcgc ggatttgggc aacgactggt
    10561 ttgtcgcccg caccggctac accggcgaag acggcgtcga agtcatcctg cccggcaccg
    10621 aagccgtcgc attcttcaaa gccctgcaac aagccggcgt acagccctgc ggcctcggcg
    10681 cgcgcgacac cctgcgcatg gaagccggca tgaacctcta cggcaacgat atggacgacg
    10741 acaccagccc gctcgaagca ggtatgggtt ggaccgttga cttgaaagac gaaagccgcg
    10801 acttcgtcgg caaagccgcc ttgctggcat tgaaagaaaa aggcgttgcc gtcaaacagg
    10861 tcggcctgtt gctcgaaaaa ggcggcatcc tgcgcgcgca tatggaagtg ttgaccgaca
    10921 aaggccaagg cgaaaccacc agcggcgtat tctccccaag cctgaaacaa tccatcgcca
    10981 tcgcgcgcgt accgaaagat tttgacggcg ataccgccaa agtgctgatg cgtggcaaag
    11041 aagtggacgt gcgtgtactg aagctgccgt ttgtccgcaa cggacagaaa cagtttgatt
    11101 gatgcggttt cagacggcat tttcatttca tatgccgtct gaaagcaggt tttaattgtt
    11161 gtccgatacg gacgtttgta gaaagcattg aacaaggcat ctgtggatat tgattcatgc
    11221 agatgccgtc tgaaaataac ccctatcaat ggagtatcaa accatgagca acaacatccc
    11281 ggccgaactg aaatacgttg ccagccatga atggctgcgc cttgaagaag acggtacgat
    11341 taccgtcggc attacccacc acgcgcaaga gctgttgggc gacatcgtgt tcgtcgagct
    11401 gcccgaagtc ggcgcgaacc ttgccgctga agagcaagcc ggtgtggttg agtctgtaaa
    11461 agccgcgtcc gacgtgtacg caccgattgc aggcgaagtc gttgccgtca acgaagattt
    11521 gccaagcgct ccggaaactg ccaacagcga tccttacggt gcaggctggt tcttcaaact
    11581 caaaccggca aacgttgccg attacgacag tctgctgact gccgaacaat acgcgggcga
    11641 agtggattaa accgcccggc tgcccgacgg caaccgccgg acaaacggaa actgcacctt
    11701 cagacggcat ttttgcggtc ggaggtgcag ttttttgtcc gtgttttaag gaagcagtta
    11761 ggctataata acggtctata ttcatcttta ccgatttttt catgcaactt accgctgtcg
    11821 gactcaatca tcaaaccgca cctttaagca tacgggaaaa gctggcgttt gccgccgccg
    11881 ccctgcctaa agccgtccgc aatcttgccc gaagcaatgc ggcaacggag gcggtaatcc
    11941 tttctacctg caaccgcacc gagctttact gcgtcggtga ttcggaagaa atcatccgat
    12001 ggcttgccga ttaccacagt ttgccgattg aagaaatccg tccgtatctg tacgcgctgg
    12061 atatgcagga gactgtgcgc catgctttcc gcgtcgcctg cgggctggat tcgatggtgt
    12121 tgggcgagcc gcagatttta ggacagatta aggatgccgt tagggttgct caagagcagg
    12181 aaagtatggg taagaaactc aatgccctgt tccaaaaaac cttttccgtt gctaaagagg
    12241 tccgtaccga tactgccgtc ggcgaaaact cggtttccat ggcttccgct tccgtcaaat
    12301 tggcggaaca gatttttccc gacatcggcg atttgaatgt cttgtttatc ggcgcaggcg
    12361 aaatgattga gctggttgcc acttattttg ccgccaaaag tccccggctg atgacggttg
    12421 ccaaccggac gctggcgcgt gcacaggagt tgtgcgacaa gctcggtgtc aacgccgaac
    12481 cgtgcctgct gtccgatctg cctgccattc tgcacgatta cgacgtagtg gtttcttcaa
    12541 cggcaagcca gttgcccatt gtcggcaaag gcatggtgga gcgtgcattg aaacaaaggc
    12601 agagtatgcc gttgttcatg cttgatttgg cagtgccgcg tgacattgaa gcggaagtcg
    12661 gcgatttgaa tgatgcctat ctttatacgg tggacgatat ggtcaatatc gtccaaagcg
    12721 gcaaggaggc aaggcagaag gccgccgccg ccgccgaaac gctggtgtcc gagaaagttg
    12781 ccgaatttgt caggcagcag cagggcaggc agagtgtccc cttgattaag gcgttgcggg
    12841 acgagggcga gaaagcgcgc aaacaggtgt tggaaaatgc catgaaacag cttgccaaag
    12901 gcgcaacggc agaagaggtt ttggaacggc tgtccgtcca actgaccaac aagctgctgc
    12961 attcgccgac ccaaaccttg aataaggcgg gggaagaaga taaagatttg gttcatgccg
    13021 tcgcgcagat ttatcatttg gacaaataac ggtgcgccgg gaaaaatgcc gtctgaagag
    13081 gtttcagacg gcatattttt gtgctgtctg acaacatcgt gaaatcccac attatatcga
    13141 tgtaatcaca aagtatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
    13201 gtacagatag tacggcaagg cgaggcaacg ctgtactggt ttaaatttaa tccactatat
    13261 tatcccgtat gcggattggt tttaagattt gtaaatttga tttgcatcaa aaaatcgccg
    13321 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
    13381 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacggcgt tttttgccgc
    13441 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
    13501 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacagcaagc cggaaggtaa
    13561 gcctatggtt gcccgcgttt acaaaggcga tgagcagttg ggcttggtct atatcacgac
    13621 cgatgcggtc aatacgcgcg gttattcgag caaaccgatc gatacgctga tggctttggc
    13681 aaatgacggc acgatagccg gagcgaaact ggtcgatcat cacgaaccga ttatgctgat
    13741 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc ttaaactggc ttccggcgta
    13801 tatcaaacca aactttatat cgacaaaccg attacgattg aagggcctgc cgaccgttcc
    13861 gcaaccatcg aaggagacag gagcgggcgt accatagccg tacacgcgcc ggacgtaacg
    13921 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc ctgcaatgga tgccggtatt
    13981 tatctcgaag aaaccgcccc gcgcgccctg atcgaacaca acaatatttt ggataattcg
    14041 gtcggcgtat atctgcatgg ttctgccgat gcgatggtgc gggagaataa aatcgtcggc
    14101 gacgcgactt tgcgcgtgaa cgagcgcggc aatggcgtta ccgtttggaa cgcgcccggc
    14161 gcgcaggtcg tcggcaacga tatttccaaa gggcgggacg gcattttttc caataccagc
    14221 acgcacaaca cctataaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
    14281 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat gggctatgtg
    14341 ctgatgtttt ccgagcggct caaagtgttt gacaatatcg ccgtcggcag ccgcgaccaa
    14401 ggcatcatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaagcg
    14461 ggcaagtgcg tttttgccta caatgccaac tacgataaac tgtccgccaa tcattttgaa
    14521 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtcact gcacgacaat
    14581 tcctttatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
    14641 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
    14701 ttcggcgaca gcgcgtaccg gcccaacggc atcatcgacc aaatcatctg gcgcgcgccc
    14761 gtatcgcgtc tcttgatgaa cagtcccgca atcagcatcg tcaaatgggc gcaggcgcag
    14821 tttcccgccg ttctgcctgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
    14881 cccaaaattc aaacccgtta tcaggcgatg aaggacgagc tactcaaaga agtcgaaacg
    14941 cggcagtcgg aatggggcag ggcggaaaac ggttctttga actagtctgc ttcagacggc
    15001 atccggattc aaatgccgtc tgaaaacaca aaaggaacaa ccatgaccac acatcatgtc
    15061 gaattgagga aggtaaccaa acggttcggg gcgcaaaaag ccgtcaacca agtcgatttg
    15121 gttttgaagg caggagaaag cgtcgggctt gccggacaca acggcgcggg caagtccacc
    15181 attatgaagc tgatactcgg gctgattacc ccgaccgaag gcgaagtgat gcttttgggc
    15241 gaacgtaccg gtagcaaagc gggggcgcgg cttcgcagcc aaatcggcta cctgcccgaa
    15301 accgttgcgc tgcacccttc gctgatcggc atcgaaacgc tggattttta tgccaaactt
    15361 aaaaaacagc cgctcacgca gaaccggggg ctgcttgagc gcgtcggcat ttcacaggcg
    15421 gcacaccgcc gcgtcggcac ttattctaaa gggatgcgcc aacgccttgc cttggcacaa
    15481 gccctgctgg gcgagcccaa agtcctgctg tttgatgaac cgacaaccgg tcttgaccct
    15541 gcatcacgac aaatgtttta cgaagtcgtg cgcgaactca acgggcgcgg cgcgaccgta
    15601 ttgctcagca cccacgccct tgccgagttg gacgggcacg ccgaccgcat tatcgtggat
    15661 taaatttaat ccactatatg cgggtatggc gggtttgagc ggacaaatca gcctgaccgt
    15721 ccccgttttg ctgaccgctc aggttttatg ggttatcatt ccgcttgttt tggcagccgg
    15781 aatttttaga aagcgacaaa tatgaaaaaa accctgttgg caattgttgc cgtttccgcc
    15841 ttaagtgcct gccggcaggc ggaagaggga ccgccgcctt taccccggca gattagcgac
    15901 cgttcggtcg gacactattg cagtatgaac ctgaccgaac acaacggccc caaagcccag
    15961 attttcttga acggcaaacc cgatcagccc gtttggttct ccaccatcaa gcagatgttc
    16021 ggctatacca agctgcccga agagcctaaa ggcatccgcg tgatttacgt taccgatatg
    16081 ggcaatgtta ccgattggac gaatcccaat gccgacacgg agtggatgga tgcgaaaaaa
    16141 gccttttacg tcatcgacag cggctttatc ggcggtatgg gtgcggaaga cgcgctgccg
    16201 ttcggcaaca aagagcaggc tgagaaattt gcaaaggata aaggcggtaa ggttgtcggt
    16261 ttcgacgata tgcctgatac ctatattttc aaataatatt gaaaaaaccg gccgaaactt
    16321 taaaaacgag tttcggtcgg tttttctttt cttgttcggt atagtgtcgg caggaaagaa
    16381 ccttcacatc ccgccgtaat tcggcccgct cgcgccttcg gggcaaatcc aagtgatgtt
    16441 ttgcgtcggg tctttgatgt cgcaggtttt gcagtgcacg cagtttgccg cgttgatttg
    16501 caggcgcgga ttgccgtttt cttcaacaat ttcgtacacg ccggccggac aatagcgcgt
    16561 ttcgggcgag gcgtattctt tgtagttcac gtctatcatc gtttgcggat tgttcagcac
    16621 caaatggtcg ggctggtttt cttcgtgcgc gagattggca aggaagacgc tgctcaagcg
    16681 gtcgaaggtc aacacgccgt cgggtttcgg ataatcaatc ggcttacacg cggcggcttt
    16741 tttaagctgc tcgttgtctt tgccgtgatg tttcaaggtc cacggggctt tgcctctgaa
    16801 aatcatctga tcgatgccgg tgtagattga gccgaggtaa acgccccatt tgaatgacgg
    16861 acggacattg cgcgcggcgt aaagctcttg atacagccag ctttgttcaa aacgttgctg
    16921 ataatccgcc gcctctttgc cgctgtcgaa accctccact tcttcaaggt tttccaacaa
    16981 ggggaacacg gcttcggcgg cgagcatggc ggatttcatc gcggtatgaa tgcctttgat
    17041 gcgcggcata ttgaggaaac ccgccgcatc gccgaccaaa atgccgcctt tgaacgagag
    17101 cttcggcaaa ctttgcaaac cgccttcaat cagcgaacgc gcgccgtaag caatgcggcg
    17161 gccgccttca aaggttttgc ggatttcggg atgggttttg aaacgttgga actcttcaaa
    17221 cggcgacaga taaggatttt gatagtccaa accgaccacg aagccgacgg cgactttgtt
    17281 gtcgtcgaaa tggtaaacaa acgcgccgcc gtaggttttg ctgtccagcg gccagcctgc
    17341 gctgtgcacc accaaaccgg gctgatgctg ttcggacggc acttcccaaa cttctttaat
    17401 gcccaagccg taagtttgcg gctggctgtt ttggtcgagt tggaaacgtt cgatgatttg
    17461 tttggaaagc gaaccgcgac aaccttcggc aaacagggtt tgctgcgccc aaagctccat
    17521 gccgggttgg aatgaatcgg tcggctcgcc gtctttgcca atgcccatat tgccggttgc
    17581 aatgcctttg accgaaccgt cttcgtgata cagcacttcg gcggcggcaa agcccggata
    17641 gatttccacg cccatatttt ccgcctgctc cgccaaccag cgcacgactt cgcccaagct
    17701 gacgatgtag ttgccgtgat tgtcgaaatt cggggtaatc ggcaggttga acgctttttt
    17761 ctcggtcagg aacaacactt tgtcctgcgt tactgtgcgt gtcagcggtg cgcctttttc
    17821 tttccagtcg gaaatcaact cattcagcgc aatcggatcg ataactgcgc cagccagcga
    17881 atgcgccccc acctccgaac ctttctccac cacgcaaacg ctgatttcgc gcccgttttg
    17941 ttcggcaagc tgcttgagtt tgatggcggc agacaaaccc gacgggcctg cgccgacaat
    18001 cacgacatcg tattgcatac tgtcgcgggt gatggattct gtcatggcgg ttcctgtgta
    18061 tttattattg aattgcaaat ccgtaattat acaacgggaa catatagtta ccaaatacaa
    18121 caaaggtcgt ctgaaaacca tattttcggt tttcagacga cctttgtcga aatttcaata
    18181 agcacgccac cattttacct gtccgaccgc aaactccgtc tgacgtttcg gactgcgtgt
    18241 gaaaaacgcc ttatccccgc cggcatccct ccctttcggc acaaccgcca aaatcttacc
    18301 tgccaaattt ccctcacggg tttgccaagc atccaaaaac tgcgccctgc tcatcgaaac
    18361 atgacccagc gacgggtcgg caagcaaaac cgtattgccg tctataccgc gcaataccga
    18421 gaaatgatca tccttgcggt atttcagata cacgatgacg gggatttgca actgtgcaag
    18481 ctgctcgaaa gacagggcat agcctttcgc ttcaaaaccc aaatcaggca taatgcgccg
    18541 catatcctca aacgacgcgg gcatctgctc cttatccagt ttttttaaca cgtcctcttc
    18601 cgtcagcttt tgcccgtaaa aattgttcaa aagcgtcgcc accgaagccg ccccgcagga
    18661 aaaatccaaa tcctgcttta caatattgaa atcgcgcctc tctttccaac tctgca
//
LOCUS       DAWHRS010000022        18086 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWHRS010000022 DAWHRS010000000
VERSION     DAWHRS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.022"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(84..1187)
                     /locus_tag="WIB82_000535"
     CDS             complement(84..1187)
                     /locus_tag="WIB82_000535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ3841878.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1267..2823)
                     /gene="dnaA"
                     /locus_tag="WIB82_000536"
     CDS             complement(1267..2823)
                     /gene="dnaA"
                     /locus_tag="WIB82_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ3841879.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VSASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAELREEDPELAQD
                     YEKLLILIQN"
     gene            3115..3249
                     /gene="rpmH"
                     /locus_tag="WIB82_000537"
     CDS             3115..3249
                     /gene="rpmH"
                     /locus_tag="WIB82_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ3841880.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3252..3617
                     /locus_tag="WIB82_000538"
     CDS             3252..3617
                     /locus_tag="WIB82_000538"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ3841881.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3682..3903
                     /gene="yidD"
                     /locus_tag="WIB82_000539"
     CDS             3682..3903
                     /gene="yidD"
                     /locus_tag="WIB82_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ3841882.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4076..5713
                     /gene="yidC"
                     /locus_tag="WIB82_000540"
     CDS             4076..5713
                     /gene="yidC"
                     /locus_tag="WIB82_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ3841883.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMVAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5794..6507)
                     /locus_tag="WIB82_000541"
     CDS             complement(5794..6507)
                     /locus_tag="WIB82_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ3841884.1"
                     /translation="MVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQSVVSKTVADWRR
                     MKEMPNLKKRPTIFVMYAG"
     gene            complement(6566..7156)
                     /gene="maf"
                     /locus_tag="WIB82_000542"
     CDS             complement(6566..7156)
                     /gene="maf"
                     /locus_tag="WIB82_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ3841885.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7304..7807
                     /locus_tag="WIB82_000543"
     CDS             7304..7807
                     /locus_tag="WIB82_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ3841886.1"
                     /translation="MSDPNLIDSEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            7841..8020
                     /gene="rpmF"
                     /locus_tag="WIB82_000544"
     CDS             7841..8020
                     /gene="rpmF"
                     /locus_tag="WIB82_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ3841887.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8195..8722
                     /locus_tag="WIB82_000545"
     CDS             8195..8722
                     /locus_tag="WIB82_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ3841888.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            8811..9866
                     /gene="plsX"
                     /locus_tag="WIB82_000546"
     CDS             8811..9866
                     /gene="plsX"
                     /locus_tag="WIB82_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ3841889.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            10002..10253
                     /locus_tag="WIB82_000547"
     CDS             10002..10253
                     /locus_tag="WIB82_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ3841890.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            10280..10488
                     /locus_tag="WIB82_000548"
                     /pseudo
     CDS             10280..10488
                     /locus_tag="WIB82_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10619..10822
                     /locus_tag="WIB82_000549"
     CDS             10619..10822
                     /locus_tag="WIB82_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841891.1"
                     /translation="MPSEAAWAFRRHFLRLKQHIGNPAPPVHGNLHLKTICMPNHTPI
                     PISVICTLPVLGNHRFFAYNSRI"
     gene            10849..11811
                     /locus_tag="WIB82_000550"
     CDS             10849..11811
                     /locus_tag="WIB82_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ3841892.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            11858..12229
                     /locus_tag="WIB82_000551"
     CDS             11858..12229
                     /locus_tag="WIB82_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ3841893.1"
                     /translation="MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            12255..13181
                     /gene="fabD"
                     /locus_tag="WIB82_000552"
     CDS             12255..13181
                     /gene="fabD"
                     /locus_tag="WIB82_000552"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ3841894.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAGHAVVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFTDAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDVDKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAVCSALTDAGQVAAFIEAH"
     gene            13319..15184
                     /gene="msbA"
                     /locus_tag="WIB82_000553"
     CDS             13319..15184
                     /gene="msbA"
                     /locus_tag="WIB82_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ3841895.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLFALQTANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            15284..16849
                     /gene="guaA"
                     /locus_tag="WIB82_000554"
     CDS             15284..16849
                     /gene="guaA"
                     /locus_tag="WIB82_000554"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ3841896.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNESKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVIALRAVITSDFMTAHWAELPYSLLGKVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            16911..17657
                     /gene="fabG"
                     /locus_tag="WIB82_000555"
     CDS             16911..17657
                     /gene="fabG"
                     /locus_tag="WIB82_000555"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ3841897.1"
                     /translation="MSTQDLNGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETIESLIADIEKAFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            complement(17929..>18086)
                     /locus_tag="WIB82_000556"
     CDS             complement(17929..>18086)
                     /locus_tag="WIB82_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3841898.1"
                     /translation="VEQSFGTLHHKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANR
                     LSAPAAA"
BASE COUNT         4496 a         5514 c         4340 g         3736 t
ORIGIN      
        1 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggcata tctccaatac
       61 gggcatcgtg ctccggaaaa ccgtcaaatt cgcatcggca tcacgatata tttgaaattc
      121 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
      181 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
      241 agttcgccgc cctgataggc aatctcgatt tcttcgcgcg cttcttcctg ctcgttgttg
      301 ctgcacacga cgctcaacag gccggattgc aggaacaggc gcgcgccacg gaatttttcg
      361 ttggcaagaa tggacacacg ttccagcgcg cccaaaagtt cggcacggga caaaacgaaa
      421 atcttgtcgt tgtccaaagg aatcacgcgg ttgaaatcgg ggaatttgcc gtcgatgacc
      481 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gtttgtccag cagctcgatt
      541 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
      601 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
      661 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
      721 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
      781 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
      841 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
      901 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagtgcccg taaaatgtcc
      961 tgaaatttct tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca
     1021 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
     1081 atcaggacgt tggacaggat gggcagggta tggcggcgtt cgacgatgcc ggtaacggct
     1141 tgcagcggct tgagcaggct gtcgcgttcg gcttgtaaaa tcaacatttt cgctccttaa
     1201 atcggttgga ttttttaccc ttcggacggc atgggcaata tcatgccgtc tgaaaggcgc
     1261 gttcggtcag ttttgaatca gaatcagcag tttctcgtaa tcctgcgcca actcggggtc
     1321 ttcctcgcgc agttccgcca ctgccctgat gccgtgcata acggtcgtat ggtcgcgccc
     1381 accgaacgaa tcgccgatag acggcaggct caaagtggtc agttctttgg tcaggctcat
     1441 cgccacctgg cgcggacggg caatgtttcg tgtccgtttc ttaccgagca catcgctgat
     1501 tttgatgcgg taatatttcg caacggcatc aataataata tcggcggtga tgactttgtg
     1561 cttctcggca ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg
     1621 gttcataaag cggctgctcg cgctgacgcg gttgaacgcg ccttcaagct cacgcacgtt
     1681 ggaacggatc agattggcaa tgaacagcgc ggcttcgtct tcaatactga tgcccgccgc
     1741 ttccgccttt ttctgcaaaa tggcgatacg catttccaat tcgggcggct cgagttccaa
     1801 agtcagtccc catgaaaaac gggatttgag gcggtcgtcc ataccttcga ttttggcggg
     1861 taaaacatcg caagtgagga tgagctgttt tttctcgtta tggaaatgat tgtacagata
     1921 gaagaattcc tccatcgtac ggtctttgcc tttgatgaac tggatgtcgt cgataatcag
     1981 caggtcgtat tgcttgtatt gctgcttgaa cacgtcgtag gtattgttgc gaaccgcctt
     2041 cataaagctg cggatatagt cgtccgaatg catatagcgc actttggcat cgggacggtt
     2101 tttcaacagt tcgttgccga ccgcctgcac aaggtgggtt ttgcccaaac ccgtactgcc
     2161 atagaggaag aacgggttgt aactctgccc cgggctttcc gcaatcgcct gcgccgcagc
     2221 cgccgcaagg cggttgccct taccttctac caacgtatca aacgtgtaat ccggagacag
     2281 gttggtctgc tcgtaacgcg cctcttccgc atcgcgctgc acgtccgtcc gtgctttggc
     2341 aactgccacc gattccggcc gggaagcaga cccggcagcc tgacgcggct cgtgcggcag
     2401 gtttttcata cgttccgcca aaatatccgc cgccgttttc gacgcagcgg gtttgacagg
     2461 ctcttcagac ggcagctcgt ccaacagaac ctcctgcacg ggcattccct ccgacaccgc
     2521 atgcaaggac ggctcggcag gttcgacagc accttcaacc gccgccatct cataacgcac
     2581 gccttctccc ggtttgaata cgaaggcgga acggccggca gccaactctt ccctcaccgc
     2641 ttctattttt ccggcaaact ggctcttgag catattgcag gcaaactggt tcttgccgta
     2701 caccacccat acgccaccct cctcaccaac ggtaaggggc gcaatccatt gcgcaaactg
     2761 cccttgaggc aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt
     2821 catggatagg ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca
     2881 aaaagaaaac aaatctgttg aacgcacatc ggttcaaaac gcgactgccc gattataccg
     2941 actcacgaat attttatcca caacccgtgc aaaaatttat ccacagaaag gcggcggaaa
     3001 tccgcaggca atcgggcaat cttcctgcaa agtttctata ttgattgaca aaagcggcaa
     3061 attggagtgt aattcacggt ttaattatct acccattcta ttttaggaaa catcatgaaa
     3121 cgcacttatc aaccttccgt taccaaacgc aaacgcaccc acggcttcct ggtgcgctcc
     3181 aaaacgcgcg gcggccgcgc agtattggcc gcacgccgtg ccaaaggccg caaacgcctg
     3241 gcggtataat tttggactac cgcttcggaa ggcagtaccg cttgttgaaa acggatgatt
     3301 tttcatccgt ttttgcattc agaaaccgcc gcagccgcga cctgctgcaa gtttcgcgct
     3361 caaacggcaa cgggctgggc catccccgca tcggtctggt ggtcggcaaa aaaaccgcca
     3421 aacgcgccaa cgaacgaaat tatatgaagc gcgttatccg cgactggttt agattgaaca
     3481 aaaaccggct gccgccgcag gatttcgtcg tgcgcgtcca ccgtaaattc gacagggcta
     3541 ccgcaaaaca ggcaagggcg gaactggcac aactcatgtt cggcaacccc gcaaccggat
     3601 gcaggaaaca ggcatgatta gaacggtact ctgcaggcaa ggttcagacg gcaacgggtt
     3661 tcccatacaa ggaacatccc gatgaacttt ctattgtcca aactcctgct gggactgata
     3721 cggttctacc aatattgcat cagcccgctg attccgccgc gctgccgtta tacgccgacc
     3781 tgttcgcaat acgcggtcga agcggtcaaa aaatacggcg cattcaaagg cggccggctc
     3841 gccatcaagc gcattgcacg ctgccaccct ttcggcggac acggacacga ccccgttccc
     3901 tgacccgacg caatattcaa attgcacgct ttccttttat ttcccatcgg tttctatata
     3961 atgccgtctg aaacttcggg ctggcggcac gaccgtcggg tatgaagccc gcccttattc
     4021 cccgtctatc ggaacacgca acctgcggca tttccgacca ttcaggaaac tctatatgga
     4081 ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg gctgggaaaa
     4141 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
     4201 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
     4261 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
     4321 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
     4381 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
     4441 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
     4501 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
     4561 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
     4621 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggctact ttacccactc
     4681 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttctccga
     4741 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
     4801 cggctggctc ggcatgattg aacaccactt catgtccacc tggatcctcc aacccaaagg
     4861 cggacaaagc gtttgcgccg ctggcgactg ccgtatcgac atcaaacgcc gcaacgacaa
     4921 gctgtacagc accagcgtca gcgtgccttt agccgccatc caaaacggcg cgaaagccga
     4981 agcctccatc aacctctacg ccggcccaca gaccacatcc gttatcgcaa acatcgccga
     5041 caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcttccc cgctcttctg
     5101 gctcctgaac caactgcaca acatcatcgg caactggggc tgggcgatta tcgttttaac
     5161 catcatcgtc aaagccgtac tgtatccatt gaccaacgcg tcctaccgtt cgatggcgaa
     5221 aatgcgtgcc gccgcgccca aactgcaggc aatcaaagag aaatacggcg atgaccgcat
     5281 ggcgcaacaa caggcgatga tgcagcttta cacagacgag aaaatcaacc cgctgggcgg
     5341 ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg cattgttcgc
     5401 ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca gccgcgccga
     5461 cccctactac atcctgccca tcattatggc ggcaacgatg gtcgcccaaa cttatctgaa
     5521 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
     5581 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
     5641 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgca cccaaggcga
     5701 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
     5761 tttattgttc accccctatc ggggcggaaa tcttcaacct gcatacatca caaaaatcgt
     5821 cgggcgtttt ttcagattgg gcatttcctt catcctgcgc caatccgcaa ccgttttact
     5881 gaccaccgac tgagtcggca acgtcaaatc cgtagccgtg cacaagcgcg tttcaggatg
     5941 caggttttcc accgcatccg ccaacaacac atcgttgcga tacggcgttt cgataaacag
     6001 ctgcgtttca ttctgctggc gcgaacgttg ttccaaagcc ttcaaactct gaatccgctc
     6061 atttttttca gacggcaaat aacccttaaa cgcaaagttc tgcccattcg cacccgaagc
     6121 catcagcgcc agcaacaagc tggacggacc gaccaaaggc cgtacttcaa aaccgtgttt
     6181 atgcgccaat gccaccaaat tcgcacccgg atcagccaca gccgggcaac ccgcctcgct
     6241 gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccggca aagtcttcaa
     6301 atccgtgtgt tcgttcagcg tttgcaaatt cagttcgcgg ataggcattg tcacgcccaa
     6361 atgtttcaaa tgcgcgcgcg ccgttttttc cgcttccacg acaaaatccg tcagcccgac
     6421 aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
     6481 aatcaaatac aaaacaggag acaccattcc ctcactcatc ggttaaaaat gccgtctgaa
     6541 cccttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaaaaaat
     6601 caaccagacg gaaaaccggc aaaccgatta aagcattcgg atcggtactc tcaatccttt
     6661 cgatcaacaa tgcacccaaa gcctcgctct tcaacgcaca cgagcaataa accgcatcag
     6721 gctcgcgctc caaatagcgg aggatgtgta gctcgtccaa cttcctcatc acgaccaccg
     6781 tcttatcgat atgccgctgc atcctgcccg taaccgtatt tagcagaacg accgcgctgt
     6841 aaaactcaat ctccctgccg ctcaaatgca tcaacatctt gtgcgcatta gcaaggttca
     6901 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
     6961 cctctgggaa acgcccggcc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
     7021 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
     7081 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
     7141 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcagtaaa
     7201 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
     7261 aaatattgac gttagaacat ctaaattata tcatatcccg tttatgtcag accctaattt
     7321 gattgactcg gaaatttttg ccgccgaagg gcagaacctg caaggcagtt ttctgctgga
     7381 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
     7441 gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa
     7501 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
     7561 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
     7621 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
     7681 ccaaatcctg atgtccctgc ccttttcgcc gcgacacgaa gactgcggcg acaatgggac
     7741 actggaagaa gtcaatcggg acaaacccaa cccctttgct gttttggcag gtttgaaaag
     7801 caattgatta ggacacagtt tatttatcta ggagcttgaa atggccgttc aacaaaacaa
     7861 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcccgcact
     7921 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatttccc ccaacggtat
     7981 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
     8041 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
     8101 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataatac cccgtgaaaa
     8161 tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta
     8221 cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg ccgacatcaa
     8281 atacgatgcc atgattgcca tcggcggcgg cggctttatt ccggcacgta tgctgcgctg
     8341 ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg acaacgaagg
     8401 acaggttacc gaagaagtca aaaaagtcca atggctcgac cccgttcccg aagccctgcg
     8461 gggcaaaaac gtactcgtcg tcgatgaagt ggacgacagc cgcgtaacca tggagttctg
     8521 cctgaaagaa ctgctcaagg aagacttcgg tacgatcgga gtcgccgtac tgcacgaaaa
     8581 aatcaaagcc aaagcaggca aaatccccga aggcattccc tatttcagcg gcatcaccgt
     8641 agaagactgg tggatcaact atccgtggga cgcactcgac atcgacgaac acaaccgcct
     8701 tgccgaggcc ggccgaggct gaccctttca gacggcatat tttccgaacc gatgccgtct
     8761 gaagcccgca cgacccctgc cgcagaccga aaacctaccg gagaaaccct atgattacat
     8821 tggccgtaga tgccatgggc ggcgaccaag gacttgccgt taccgtaccc ggcgcaaccg
     8881 cattcctcca agcacacccc gatgtccgcc tgattatgac cggcgacgaa acgcaactgc
     8941 gccaagccct gaccgcggca ggcgcaccga tggaacgcat cgacatctgc cataccaccc
     9001 aagtcgtcgg catggacgaa gccccgcaat ccgccctgaa aaacaaaaaa gactcctcca
     9061 tgcgcgtcgc catcaaccag gttaaagaag gcaaagccca agccgccgta tccgcaggca
     9121 acacgggtgc gctcatggca accgcacgtt tcgtcctcaa aaccattccc ggcatcgaac
     9181 gccccgccat cgccaaattc cttccttccg acaccgacca cgttaccctt gcactcgacc
     9241 ttggcgcgaa cgtcgactgc acgcccgaac agctcgccca atttgccgtt atcggcagcg
     9301 aactcgtcca cgcactccat cctcaaaaag gacagccgcg cgtcgggctg gtcaacgtcg
     9361 gcacggaaga catcaaaggt acggacaccg tcaaacaaac ctacaaactg ctgcaaaaca
     9421 gcaaactcaa ctttatcggc aacatcgaaa gcaacggcat cctctacggc gaagcagatg
     9481 tcgtcgtcgc cgacggcttt gtcggcaacg tcatgctcaa aaccatcgaa ggcgcggtca
     9541 aattcatgag cggagccatc cgccgcgaat tccaaagcaa cctgttcaac aaacttgccg
     9601 ccgttgccgc cctacccgcc ctcaaagggc tgaaaaacaa actcgatccg cgcaaattca
     9661 acggggccat cctgctcggg ctgcgcggca tcgtgattaa aagccacggc ggcacagacg
     9721 aaaccggttt ccgctacgca ctcgaagaag cctaccacga agccaagtcc gccggccttt
     9781 ccaaaatcga acagggcgta gccgaacaac tcgccgcact cgaaactgcc aaagccgtcc
     9841 aaaacgaaaa tgtcggcggt ctgtaacacg cgcgatgccg tccgaacgcc cccgcccctt
     9901 tcagacggca tccgcccgcc ccgaacctgc gggcgcggac ggcgatgcac ctgtccggca
     9961 cttcccaaat acttccctgt aaaataagga atatttgaaa aatgaagaca ttagaaaaac
    10021 ggatgaaagc tctagacaaa cggatgatga agttcggaaa atcccttgaa ggcaggcttg
    10081 atgcccgtct gattgaatcc gcattggatt atattcatta ttcggaacgt tttttggctt
    10141 ttgaaatcct gtgtacttat atcgaagatt tcgatgtccg gctgacggaa caagaatccc
    10201 gggaaatttc ttttatcaac aaggaatttg agatagaaag cacgtccgat taaccaataa
    10261 agccaatggg ttgataaaca tgaaaacatc gacggtcgtt tttggcggat tttttatggc
    10321 agacaacgga gagcgaatcc aaatccccgt tttggaaaat cctgacatta gggaaatcaa
    10381 tcactttttt ccgtatcaaa ttttgagaaa aaagccggcg tccttgtttt cagaatcatc
    10441 cccgagccgg aatttggcga taccgaatta actgtctatt ttgaaaagga tattatagtg
    10501 gattaacaaa aaccagtacg gcgttgcctc gccttgccgt actggttttt gttaatccac
    10561 tatatcagac gaaaacaaac acccgcgcca atagcctgac ggcaacccgg caatcaaaat
    10621 gccgtctgaa gcagcttggg ctttcagacg gcatttcctc cgcttaaaac agcatatcgg
    10681 caaccccgcc ccgcccgtcc acggcaatct gcatctgaaa accatctgta tgccaaacca
    10741 cacccccatc ccgatttccg tcatttgcac cctgcctgta ttgggcaatc atcggttttt
    10801 cgcttacaat agccgaatct gaaccaacta tccaaaaagg ccgttcccat gcagtatgcc
    10861 aaaatttccg gcacgggcag ctatcttcct gccaaccgcg tcagcaatga cgaccttgcc
    10921 caaaaagtgg atacctcgga cgagtggatt accgcgcgca cgggcatcaa attccgccat
    10981 attgcagccg aaaacgaaaa aaccagcgac cttgccgccg aagcggcgcg gcgcgctttg
    11041 gcagatgcca aattaaatat aaacgacatt gatttgatta tcgtggcgac ggcaacgccg
    11101 gatatgcagt ttccttctac cgccaccatc gtgcagcaaa aattgggcat caccaacggc
    11161 tgtcccgcgt ttgacgtaca ggcagtgtgc gcaggcttta tgtacgcgct gaccacggca
    11221 aacgcctaca ttaaaagcgg tatggcgaaa aacgcgctgg tcatcggcgc ggaaaccttc
    11281 agccgcatcg tcgattggaa cgaccgcaca acctgcgtat tgttcggcga cggcgcgggc
    11341 gcggtggttt tgagcgcggc ggacaaaccg ggcatcatcc acagcaaact caaagccgac
    11401 ggcaattatc tgaaactctt aaacgtcccc gggcaaatcg cctgcggcaa agtttccggt
    11461 tcgccgtaca tttcgatgga cggtcccggc gtgttcaagt ttgccgtcaa aatgctgtcc
    11521 aaaatcgccg atgacgttat cgaagaagcc ggttataccg ccgctcaaat cgactggatc
    11581 gtgccgcatc aggcaaaccg ccgcattatc gaatcgaccg cgaaacattt aggtttgagt
    11641 atggacaaag tcgtcctgac cgtccaagac cacggcaaca catccgccgc atcgattccg
    11701 ctggctttgg atacgggcat ccgcagcgga caaatcaaac gcggtcaaaa cctgctgctc
    11761 gaaggcatcg gcggcggttt cgcgtggggc gcggtgctgt tgcaatattg aacccgatgc
    11821 cgtctgaaac aggctttcag acggcatttc ccatatcatg aagcggcagg ctttcttcaa
    11881 actgatggcg tgtgcggcat ttctgtctgc cgtttcgctg cgcctccccg tattgggcgc
    11941 gtgttacgca atattgtccc tctatgcgtt tgcactttac ggcatcgaca aacggcgtgc
    12001 cgtgcgagga aaacgccgca ttcccgaaca ccgcctgctg ctgcctgcct tgttcggcgg
    12061 ttggacgggc gcatacttgg gcagcaggat attcaggcat aaaacggcga aaaagcgttt
    12121 tgttgtgctg ttccgtctga ctgtttcggg caatgtccta gcgacctgca tcctgattga
    12181 ttatttcgtt ccgcccgaac tttttgtaaa actcgggcaa catctctgac aatccaaccc
    12241 aacaaaggaa tactatgtct tttgccttct tttttcccgg acaaggttcc caaagcctcg
    12301 gtatgatgaa cggctttgcc ggacacgccg tcgtcaaaaa cacctttgcc gaagcctccg
    12361 ccatattggg gcaggacttg tgggcgatga ttaacggcag cgatgccgaa atcatcggtc
    12421 aaaccgtcaa cacccagccc attatgctcg ccgccggcgt tgccgtttac cgcgcctatt
    12481 tggaagcggg cggcaaaacg cctgccgccg ttgccggaca cagcctcggc gaatacaccg
    12541 cactcgttgc cgccggcgca ttggatttta ccgatgccgt caaactcgtg cgcctgcgcg
    12601 ccgaactgat gcagtccgcc gtaccgcaag gcgtgggcgc aatggcggcg attctcggct
    12661 tggaagatga acaggtaaaa caaatttgcg ccgaatccgc acaggatgaa gtggttgaag
    12721 ccgtcaactt caactcgccc ggacaagtcg tgattgcagg caatgccgcc gccgtcgagc
    12781 gcgctatgct tgccgccaaa gaagccggcg ccaaacgcgc cctgccgctg cccgtgtccg
    12841 taccttccca ttgcagcctg atgaaacccg ccgccgacaa acttgccgaa gccctgaaaa
    12901 ccgttgaaat caagcagccg caaatccgcg tcatccacaa cgccgacgtt gccgcctacg
    12961 atgatgtcga caaaatcaaa gacgcgctcg tccgccagct ttacagcccc gtacgctgga
    13021 cggaaaccgt caacgccctc gtttcagacg gcattgccga atccgccgaa tgcggcccgg
    13081 gcaaagtgtt ggcgggcttg gcaaaacgca tcaacaaagc cgccgtgtgc agcgcgctga
    13141 ccgatgccgg acaggttgcc gcctttatcg aagcgcactg acttcgttcc gcaaaaagca
    13201 gcctgccctc ttcaggctgc ttttcacgtc cgaacggcgg cagccccata tttacgctat
    13261 aatccatccc gaccaaacca ccgacagcgg ctgccgttgc agttcccgcc ccaccgatat
    13321 gatagaaaaa ctgactttcg gactgtttaa aaaagaagac gcgcgcagct ttatgcgcct
    13381 gatggcatac gtccgcccct acaaaatccg catcattgcc gccctgattg ccattttcgg
    13441 cgttgccgcc accgaaagct accttgccgc cttcatcgcc cccctgatca accacggctt
    13501 ttccgcgccc gccgcgccgc ccgagctgtc tgccgccgcc ggcatcattt ccaccctgca
    13561 aaactggcgc gaacagttta cctatatggt ttgggggacg gaaaacaaaa tctggaccgt
    13621 cccgctcttc ctcatcatcc tcgtcgtcat ccgtggcatc tgccgcttta ccagcaccta
    13681 tctgatgact tgggtctccg tgatgaccat cagcaaaatc cgcaaagata tgtttgccaa
    13741 aatgctgacc ctttcctccc gctaccatca ggaaacgccg tccggcaccg tactgatgaa
    13801 tatgctcaac ctgaccgaac agtcggtcag caacgccagc gacatcttca ccgtcctcac
    13861 gcgcgacacg atgatcgtta ccggcctgac catcgtcctg ctttacctca actggcagct
    13921 cagcctcatc gtcgtcctga tgttccccct gctctccctg ctctcgcgct actaccgcga
    13981 ccgtctgaaa cacgtcattt ccgactcgca aaaaagcata ggcacgatga acaacgtgat
    14041 tgccgaaacc catcagggac accgcgtcgt caagctgttc aacgggcagg cgcaggcggc
    14101 aaaccggttc gacgcggtca accgcaccat cgtccgcctc agcaaaaaaa tcacgcaggc
    14161 aacggcggca cattccccgt tcagcgaact gatcgcctcg atcgccctcg ccgtcgtcat
    14221 cttcatcgcc ctgtggcaaa gccaaaacgg ctacaccacc atcggcgaat ttatggcatt
    14281 catcgtcgcg atgctgcaaa tgtacgcccc catcaaaagc cttgccaaca tcagcatccc
    14341 tatgcagacg atgttcctcg ccgccgacgg tgtatgtgca tttctcgaca ccccgcccga
    14401 acaggacaag ggcacgctcg caccgcagcg tgtcgaaggg cgcatcagct tccgcaacgt
    14461 cgatgtcgaa taccgttcag acggcatcaa agccctcgac aacttcaacc tcgacatcag
    14521 acaaggcgaa cgcgtcgccc tggtcggacg ttccggcagc ggcaaatcca ccgtcgtcaa
    14581 cctgctgccc cgctttgtcg aaccgtctgc cggcaacatc tgcatagacg gtatcgacat
    14641 cgccgacatc aaactcgact gcctgcgcgc ccaattcgcc ctcgtctccc aagacgtatt
    14701 cctgtttgac gacaccctgt ttgaaaacgt ccgatacagc cgtcccgacg cgggcgaagc
    14761 cgaagtcctg ttcgccctcc aaaccgccaa cctgcaaagc ctgattgaca gctccccgct
    14821 cggactgcac cagcccatcg gatcgaacgg cagcaactta tccggcggac agcggcaacg
    14881 cgtcgccatt gcccgcgcca ttttgaaaga cgcgccgata ttattattgg acgaagccac
    14941 cagcgcatta gacaacgaat ccgaacgcct cgtccaacag gcgctcgaac gcctgatgga
    15001 aaaccgcacc ggcatcatcg tcgcccaccg cctgaccacc atcgaagggg ccgaccgcat
    15061 catcgtgatg gacgacggca aaatcatcga acaaggcaca cacgaacaac tgatgtccca
    15121 aaacggttac tacacgatgt tacgcaatat ctcaaacaaa gatgccgccg tccggacggc
    15181 ataaacaaaa tgccgtccga aatggtacaa tcgccccgac cctttcagac ggcatcatat
    15241 ccgccgaccc atccgattat cttcaatcac tgtaaaaccc attatgaccc aagacaaaat
    15301 cctcatcctt gacttcggtt cgcaagttac ccagctcatc gcccgccgcg tgcgcgaagc
    15361 ccacgtttac tgcgagctgc attctttcga tatgcctttg gacgaaatca aagccttcaa
    15421 ccccaaaggc atcatcctct ccggcggccc caattccgtt tacgaatccg actatcaagc
    15481 cgataccggt atttttgatt tgggcattcc ggttttgggc atctgttacg gcatgcagtt
    15541 tatggcgcac cacttgggcg gcgaagtgca gcccggcaac cagcgcgaat tcggttatgc
    15601 gcaagttaaa accatagaca gcgagctgac acgcggcatt caagatggtg agccaaacac
    15661 actcgacgta tggatgagcc acggcgacaa agtgtccaaa ctgcccgacg gtttcgccgt
    15721 catcggcaac accccgtcct gcccgattgc catgatggaa aacgccgaaa aacaattcta
    15781 cggcatccag ttccaccccg aagttaccca caccaaacaa ggccgcgccc tgttgaaccg
    15841 ctttgtcttg gatatttgcg gcgcacaacc gggctggacg atgccgaact acatcgaaga
    15901 agccgttgcc aaaatccgcg aacaggtcgg cagcgacgaa gtgattttag gtctgtccgg
    15961 cggcgtggac tcttccgtag ccgccgcgct gattcaccgc gccatcggcg accaactgac
    16021 ctgcgtgttc gtcgatcacg gtttgttgcg cctgaacgaa agcaaaatgg tgatggatat
    16081 gttcgcccgc aacttgggtg tgaaagtgat acacgtcgat gccgaagggc agtttatggc
    16141 gaaactcgcc ggcgtaaccg accccgagaa aaaacgcaaa atcatcggtg cggaatttat
    16201 cgaagtattt gatgccgaag aaaaaaaact taccaacgcc aaatggttgg cacaaggcac
    16261 gatttaccct gacgtaatcg aatccgcagg tgcaaaaacc aaaaaagccc acgccatcaa
    16321 atcgcaccac aacgtcggcg gcctgcctga aaacatgaag ctcaaattgc ttgagccttt
    16381 gcgcgatttg ttcaaagacg aagtacgcga attgggtgtg gctttgggcc tgccgcgcga
    16441 aatggtgtac cgtcatccgt tcccgggtcc gggtttgggc gtgcgtattt tgggcgaagt
    16501 gaaaaaagaa tatgccgacc tgcttcgtca ggcagacgat attttcattc aagaattgcg
    16561 caatactacc gatgaaaacg gtacatcttg gtacgacctg accagccagg cattcgccgt
    16621 gttcctgccc gtcaaatctg tcggcgtaat gggcgacggc cgcacatacg attacgtcat
    16681 tgccttgcgt gccgtgatta ccagcgactt tatgaccgcg cattgggcgg aactgccgta
    16741 ttccttgttg ggcaaagtgt ccaaccgcat catcaacgaa gtcaaaggca tcaaccgcgt
    16801 ggtttatgat gtgagcggca aaccgcctgc caccatcgag tgggaataaa cagcaaacat
    16861 ggctgccccg tccggcgcag tccttcgatt atcggaaaaa aggaaaaaat atgagcacac
    16921 aagatttaaa cggcaaaatc gctttggtaa caggcgcatc gcgcggtatc ggtgcagcaa
    16981 ttgccgacac gctggcggca gccggtgcca aagtcatcgg tacggcgacc agtgagagcg
    17041 gtgcggcggc gattagcgag cggttggcgc aatggggcgg cgaaggccgc gtattaaatt
    17101 ccgccgaacc tgaaaccatc gaaagcctga ttgccgacat cgaaaaagcg ttcggcaaac
    17161 tcgatattct ggtcaacaac gccggcatca cccgcgacaa cctcctgatg cgcatgaaag
    17221 aagaagagtg ggacgacatc atgcaggtca acctcaaatc cgtgttccgc gcttctaaag
    17281 ccgttttgcg cggtatgatg aaacaacgtt ccggccgcat catcaacatc acatccgtcg
    17341 tcggcgtgat gggcaatgcc ggtcaaacca actatgccgc ggcaaaagca ggcttaatcg
    17401 gtttctccaa atccatggcg cgcgaagtcg gcagccgggg cattaccgtc aactgcgtcg
    17461 cccctggctt tatcgatacc gacatgacac gcgccctgcc ggaagaaacc cgccaaacct
    17521 ttaccgccca aaccgccttg ggcagattcg gcgacgcgca agacatcgcc gatgcggttc
    17581 tgttcctcgc ttccgaccaa gcaaaataca tcaccggcca aacgctgcac gtcaacggcg
    17641 gtatgctgat gccttaacag acaacttttt caaccatgcc gtctgaagcc ctttcagacg
    17701 gcatttgcat tctcaggcaa aatgaacaca caccacaccc cgccctgccc atgcggctca
    17761 ggcacaagct gagacctttg caaaattcct ttccctcccg acagccgaaa ccccaacaca
    17821 ggttttcagc tgttttcagc tgttttcgcc ccaaataccg cctaattcta cccaaatacc
    17881 cccttaatcc tccccggaca cctgataatc aggcatccgg gtcacctttt aggcggcagc
    17941 gggcgcactt agcctgttgg cggctttcaa aaggttcaaa cacatcgcct tcagatggct
    18001 ttgcgcactc actttaatca gtccgaaata ggctgcccgg gcgtagcgga atttatggtg
    18061 cagcgtaccg aagctctgtt cgacca
//
LOCUS       DAWHRS010000023        17985 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWHRS010000023 DAWHRS010000000
VERSION     DAWHRS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17985
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.023"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(121..2910)
                     /gene="ileS"
                     /locus_tag="WIB82_000557"
     CDS             complement(121..2910)
                     /gene="ileS"
                     /locus_tag="WIB82_000557"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ3841899.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDRPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLAGFEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGISILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETLRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMASYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTHDVGAVAGHETICKR
                     CAGNVGGEGETRHYA"
     gene            complement(3051..3971)
                     /gene="ribF"
                     /locus_tag="WIB82_000558"
     CDS             complement(3051..3971)
                     /gene="ribF"
                     /locus_tag="WIB82_000558"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ3841900.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            complement(4270..5565)
                     /gene="tyrS"
                     /locus_tag="WIB82_000559"
     CDS             complement(4270..5565)
                     /gene="tyrS"
                     /locus_tag="WIB82_000559"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ3841901.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLHQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEVLVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(5628..7496)
                     /locus_tag="WIB82_000560"
     CDS             complement(5628..7496)
                     /locus_tag="WIB82_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ3841902.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTTILHREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSLRVQFGFLAALCALSLAASFIPSALDKYYLPHLRACELLIGSLTAVWMRCRQPAV
                     GRRCAAVGALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKF
                     FQSKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAVVLILLLSLFSYHCI
                     EKPFKKWQGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNT
                     GKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASR
                     VFQNWAACRAVYRYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHK
                     LSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAK
                     YPNVYIIDAAAYIPADFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(7599..7824)
                     /locus_tag="WIB82_000561"
                     /pseudo
     CDS             complement(7599..7824)
                     /locus_tag="WIB82_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7862..8953)
                     /gene="ychF"
                     /locus_tag="WIB82_000562"
     CDS             complement(7862..8953)
                     /gene="ychF"
                     /locus_tag="WIB82_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ3841903.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9066..10742)
                     /locus_tag="WIB82_000563"
     CDS             complement(9066..10742)
                     /locus_tag="WIB82_000563"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ3841904.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLLQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDIGDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            10917..11348
                     /locus_tag="WIB82_000564"
     CDS             10917..11348
                     /locus_tag="WIB82_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ3841905.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGLNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLNERPSTQEWIGLGLVTAGVLVLALKR"
     gene            complement(11594..12289)
                     /locus_tag="WIB82_000565"
     CDS             complement(11594..12289)
                     /locus_tag="WIB82_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ3841906.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12305..12805)
                     /gene="hpaC"
                     /locus_tag="WIB82_000566"
     CDS             complement(12305..12805)
                     /gene="hpaC"
                     /locus_tag="WIB82_000566"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ3841907.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRKARIIPILSENGSLCINTLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(12920..13360)
                     /gene="nadR"
                     /locus_tag="WIB82_000567"
     CDS             complement(12920..13360)
                     /gene="nadR"
                     /locus_tag="WIB82_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ3841908.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(13703..14212)
                     /locus_tag="WIB82_000568"
     CDS             complement(13703..14212)
                     /locus_tag="WIB82_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ3841909.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAILVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14291..14366)
                     /locus_tag="WIB82_000569"
     tRNA            complement(14291..14366)
                     /locus_tag="WIB82_000569"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14331..14333),aa:Lys,seq:ttt)
     gene            14577..15656
                     /gene="apbC"
                     /locus_tag="WIB82_000570"
     CDS             14577..15656
                     /gene="apbC"
                     /locus_tag="WIB82_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ3841910.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPETGDTHIHLSMNTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            16913..17985
                     /locus_tag="WIB82_000571"
                     /pseudo
     CDS             16913..17985
                     /locus_tag="WIB82_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250668.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
BASE COUNT         4151 a         4903 c         4847 g         4084 t
ORIGIN      
        1 tgcattccga caacaccgca atcttgaaac ccctccgccg ttataaagac aaatcgcggt
       61 acaaaaaatg ccgtctgaaa tgctgtttgg cggtttcaga cggcatttgc tcaaacttta
      121 tcaggcgtaa tggcgcgttt cgccttctcc gccgacattc ccggcgcagc gtttgcagat
      181 ggtttcatgg cctgcaactg cgcccacatc gtgggtgtag tgccaacagc gttcgcattt
      241 ttcaccatcg ctggctttgg cggcaacggc aagttcatca cctactttca cttcggcttt
      301 agacaccagc agggcaaagc gcaattcttc gcccaaagcg ttcaggtagc tggccatttc
      361 ttccggcgca gtgatttcgg cttccgcctg caaggacgaa ccaacggttt tgtcggcgcg
      421 caaaggctca atggcggcgg ttaccgcttc gcgggcttcg cggattgccg tccatttttt
      481 caccagttcg gcttcggctt tttcgttgat ggtcgggaac tcgtgccaag tatggaaaag
      541 gacgctgtct tcttcgccgc cgccgatgat gtcccacgct tcctcgccgg tgaagcacaa
      601 aatcggtgca atcaagagaa ccaaactgcg tgtgatgtga tacagggcag tttgtgcgct
      661 gcggcgtgca tggctgtctg ctttggtggt atagaggcgg tctttcagga tgtcgaggta
      721 gaacgcgccc aagtcttccg agcagaaaga aacaatgtct tttacggcga agtggaaggc
      781 gtagcgcgga tagtaatcgc ctgccagacg ctcttgcagc cgacgcgcca acaccagggc
      841 atagcggtcg atttccacca tatccgcctg ttgcacggca tcttcaatcg gattaaagtc
      901 gctcaagttg gcaaacaaaa agctcaaggt attgcggata cggcggtagc tttcggttac
      961 gcgcttaagg atttctttag aaatcgccaa ttcgccgctg taatcggtag atgccgccca
     1021 cagacgcagg atgtccgcgc cgaattcgtt atagacttct tgcggcgcga cgacgttgcc
     1081 gatggatttc gacatttttt tgccttcgcc gtctacaaca aaaccgtggg tcagcagctg
     1141 tttatacggt gcgcgtccca ttgatgaggc gcagccggtc agcatggacg attgaaacca
     1201 gccccggtgc tggtcgctgc cttcgagata caaatccgcc ggccattcca attcttcgcg
     1261 ttgtttcaca acggaataat gggtcgaacc ggaatcgaac catacgtcca tcgtatcggg
     1321 gagtttgtcg taatgttcgc aatcttccgc gcttaataat tcgcttttat cgagggagaa
     1381 ccaagcctcg atgccttttt cttcgatttt cagggcaact ttttccaaaa gttcggcaga
     1441 gttcggatgc agctcgcccg tttctttgtg aacaaagaaa gtcatcggcg tgccccaata
     1501 gcgttggcgt gaaaccaccc agtcaggacg accttcaatc atggcttcca aacgcgcgcg
     1561 accccaagac gggaagaatt cggtgtcgtc cacggccttg atggctttgt cgcgcagggt
     1621 tttgccgtcg gcaccggctt tgtccatacc gacaaaccat tgaccggtcg cgcggtaaat
     1681 cagcggcgtt ttgtgccgcc agcagtgggc gtagctgtgt tcgattttac tgcttgccaa
     1741 aaggttgccg gtttcttcca accattgcag gatgacgggg ttcgcctccc aaacgcgcat
     1801 accggcaacg cgcagcgttt cgccgatgta tttgccttcg gcgttgacag gattgtaaag
     1861 ctcgatgccg tatttgttgc agacggcgta gtcttccaaa ccgtgcgcgg gcgcagtatg
     1921 caccaagccg gtaccggcat cggtggtaac gtgttcgccg ttaagcatgg gaatatcgcg
     1981 ttcgaggaac ggatggttca tgtgcaggtt ttccagcttg tcgccggtgg tttcggcaag
     2041 aatagaaatg ccgtctgaaa agccgtagcg tttcaaagcg tcttccgcca aatctttggc
     2101 caataccaat ttgcctttgg gcgtatcgat taattgatac accacgtctg cacccgctga
     2161 aacagcttgg ctcgcaggca aagtccaagg cgtggttgtc caaataacgg caaacgcttt
     2221 gccttcgaaa ccagccaaac cgaatgcggc ggcaagcgcg gcggtgtctt taaacggata
     2281 ggcaacgtca atcgcgggcg agattttgtc tttatattcc acttccgctt cggccagcga
     2341 agaaccgcag tccaagcaga attgaaccgg tttcgcaccc cggtagagat agccggattt
     2401 gtagatttcg ccgagcatac gcacggtatc ggcttcggtt ttgaaatcca tggtcaggta
     2461 aggacggtcc caatcgccca acacgcccaa gcggataaag tcttttttct ggcgggcaac
     2521 ttgttcggcg gcatattcgc ggcacaattc gcggaaacgc gctttgggca tgtctttgcc
     2581 gtgcagtttt tctaccatca cttcgatggg caggccgtgg cagtcccaac ccggcacata
     2641 aggcgcgtca aaaccggctt gggttttgct gcggataatg atgtctttga gaattttatt
     2701 gacggcatga ccgatgtgga tgtcgccgtt ggcatacggc gggccgtcgt gcaggatgaa
     2761 tttcggacgg cctttggcga tttcgcgcag tttttggtag cgtttttgct cgtaccagct
     2821 tttcagccat gcaggctcgc gcttggcaag attgccgcgc atcgggaacg ggctttcgag
     2881 caggttgacg gttttgctgt aatcggtcat tttttaatct ctattgttac aatatttcgg
     2941 ttcagacggc attgcgcctc aaacagtatt tcacaacggg aaacccctat gccgtctgaa
     3001 cagttaaaag attgattgta gcccaatcgg atggtttgta taaggttttt ctaccaacac
     3061 tttgcggctt ccatatcggc tgctatctgc cttttcagtt ctgccatacc gtcaaacttt
     3121 tcttcatcgc gcagtttgtg caggaagcgg acgttcagcc gttgtccgta caggtcgcct
     3181 tgaaagtcga acaggtggac ttcaagcttt tgagaacagc cgctatcaac ggtgggattg
     3241 aaaccgaagc tcgccacgcc gcgccgcgtg ccgaatgcgc cgtccgcttc gacgacgaac
     3301 acgccgccga gtgcataacg gtggcggggc aggcggatgt tggcagtcgg ggcgtttaag
     3361 gtgcgtccga gttttctgcc gtgcaccacc ctgccgctca agacgtagtc gtgtcccaaa
     3421 agttttttcg cataggcaag gttgccgtct gaaagggctt gtcgcacggc ggtactgctg
     3481 gtgcggatgt cttcgacgat gacggaaggc gtacgctcgg tctgcatatc gggctgttgt
     3541 gccaaaagtt caaaacagcc ttcccgcccc gcaccgaaac ggaaatcatc gccgacgagc
     3601 aaataacgcg tattcaaggt ttgacgcagc aggcggtcga taaacccttg cgcggatatt
     3661 tcggaaaaat tttgatcgaa acgcaaaacc cagacggcat cgacacagcc tgtgccttcc
     3721 aataattcga gcttggtgcg cagggggctg atccgacacg gtggcatcct gccggtgcgg
     3781 agtgcgaaaa attctttggg ttggggttcg aaaacgacgg tcacgacggg taatccgcgc
     3841 gtatcggctt cgaggcggag tttttggagg atgtgtttgt gtccaaggtg tacgccgtcg
     3901 aaattgccta tggttacggc ggccccgtgc ggaaagtcgg gcgcgttgtg ccgccccagc
     3961 ctgattctca ttgttgcgtt cgggtatgtg gtgaaacagg cggtcattgt aaacggtatt
     4021 gcggtttata gacagtgtgc cgcccgttac gcccgcccga cgctggaaaa gcaggcaaat
     4081 ttcccgccgc cgaacgcgcc aaacgcataa agcgtgcatc aggcgcgatc ctatgtgttg
     4141 aaacatcgcc ccaaacctga tgcaacatca gtgaaaatcg ttctttttta accaggtaaa
     4201 ccgagacaaa caaccctccg ccgtcattcc cgcgcaggcg ggaatgacgg catttctgcg
     4261 gcaatcggat tatttccaaa ccaaaagcgc gtggttgcgt ttgccgcgcc gaaggatggt
     4321 gtatttgccg aaacgtttgt gttcgtcggt cagcaggcag gcatcgtcgg ggcgttcggc
     4381 ggcgtggttg gggttgttgg cttcggcagg tttgccgttg agcaaaaccg ctttgctgtt
     4441 cacaaagccg cgcgcttctt tattggagga tgccaaaccg gtttttacca agacttcgac
     4501 gacattgatg ccgtctgaaa cttcaaatgc aggcagaccg tcgagggcga gctgctcgaa
     4561 gtcgctttcg gtcaggctgc tttggtcttc ggcaaacagg ctttcggaaa tgcgttgcgc
     4621 ggcggcaagg gcttcttcgc cgtgaatcag gcgggtcatt tcttcggcga ggatgcgttg
     4681 cgcttcgggc ttgctgccgc ttgccttgtc tttggcttcg atggcatcga tttcttcgat
     4741 ggacaggaag gtaaagtatt tcaggaattt atacacatcg gcatcggcga ctttcagcca
     4801 gaattggtag aactgatagg gcgaggtttt tttcgcgttc agccataccg cgccgccttc
     4861 ggttttgccg aatttggtac cgtctgattt ggttaccaaa ggcagggtca gaccgaatac
     4921 ttgtttttgg tgcaggcggc gggtcaggtc gataccggcg gtgatattgc cccattggtc
     4981 ggagccgccg atttccaaaa ccgcgccgtg gcgtttgttc aactcggcga agtcgtaacc
     5041 ttgcagcagg gaataggcga actcggtgaa ggaaatgcct gcgccgtcgc ggtcgatgcg
     5101 ctgtttgacg gattctttgt tcagcatggc gttgacggag aaatgcttgc cgatgtcgcg
     5161 caggaagtca aggcagttca tgctgccgaa ccagtcggca ttgttcgcca taatggcggc
     5221 atttccgcct tcaaagctca agaaaggggt taattggttg cggatacttt ccacccagcc
     5281 ggcaacagtt tcggcggaat tcaagctgcg ttcggcggct ttgaagctgg ggtcgccgat
     5341 cataccggtc gcgccgccca ccaaagcaat cggcgtatgc cccgcctgtt ggaagcggcg
     5401 caatgccaat acgggcagca ggtgtccgat gtgcaggctg tcggcggtcg ggtcgaagcc
     5461 gcaataaagg gcaatttttt gttcgttcaa caaagcgtct aaggcttcga tgtcggtggt
     5521 ttgcgcgata aggccgcgcg attgcaggtc ttggatgacg ctcatcggtc tctttcaaaa
     5581 aaaattagcg tttttgcaaa ccgccgattg taacaaattt aagcgaatca atggttatgg
     5641 cgcgtatcga gaaaccgttg tttttcggaa aaacgctttg ccaattccgt gccgccgtaa
     5701 gggttgatgt ggtctttgtc cgagtaaacc ggcaatccgc cgatttgaaa atctgcgggg
     5761 atataggcgg cggcatcaat aatatagacg ttggggtatt tggctgccaa ttccctgatg
     5821 cgtgcattgg ctttcagggt gctttcgtcg tccgggcgca gggcttggcg gtaacccggt
     5881 atgcgtgaag acaagatata ggcgcgctgg acgttgtaag acgaggcaag gttgtccgcc
     5941 atcaggtaaa cggcttgttt ttcggacgag agtttatgca gcatacggtc gaatttttgg
     6001 aaaaaaccgg catcataggc aagggagcgg ctgttttcgg gcatttggct gccccagcgc
     6061 atcgccaaaa ccacttttga ataccggggc aggtgttctt cggcatagcg ataaacggcg
     6121 cggcaggctg cccagttttg gaacacacgg gacgcgtagc cttccacata ggcgcaagcg
     6181 tcggcggaaa ccatagtggc ggaccatttt tcttttttgc ccacggcatc gaagaatgtt
     6241 ttgtaatggt cggcgtggga gtcgcccaaa accagcagtt ccggctgttt ttccgtatcc
     6301 ccccataggc attgtttgcc ggtattgttg tggcaggagg tgttggaacg cgtcagcccc
     6361 aagcggtcgt attgcgccat aaacggcagc ctcatcgcga aaaacgagcc cgcccccaaa
     6421 atgagcatag gcaaggcata aatccataaa acggattgtg cgaacgaacc ttgccatttt
     6481 ttaaacggtt tttcgatgca gtggtaagaa aacagggaaa gcagcaatat caggacgacc
     6541 gccgccgccg gcgaataagg cggcaggttg tccgggccga tatagcgcat aaaggccaat
     6601 atcggccaat gccacagata aagcgaatag gaaatcaaac cggcggcaac agtgattttc
     6661 gattggaaaa attttttaag cgggtgttcg taatgattga aataaatcag cgcggcaaca
     6721 gccagacagg gaatcaaagc ggcggggccc gggaaatagg cggtttgttc cgaataggaa
     6781 aacaggcagg ttgacaatat gcacacggca aacaatgcgc cgacggcggc acagcgtctg
     6841 ccgacggcag gttgccggca gcgcatccac acggcggtca gcgatcctat cagtaattcg
     6901 caggcgcgca ggtggggcag gtaatattta tcgagcgcgg aaggtataaa ggaggcggca
     6961 aggcttaagg cacacagtgc ggcaaggaag ccgaactgta cgcgcaggct tttgcgggcg
     7021 acaagcagca gcagtatcgg aaagacaaag taaaattgtt cttcgaccga caaagaccag
     7081 atgtgcagca ggggcttttc ttcctgcgcg ggatcgaaat aatccttccc ccttgcaaaa
     7141 tacaggttag aggcgaaacc caaggcggtc agcgcggatt tccacaaaag aaagaaatca
     7201 tctttggtga ataaaaagaa gccgcctgcc agcgttgccg ccaatacggc gaaaaatgcg
     7261 ggcagaatcc gcttgatgcg gcggatataa aatgccttca gggaaaacct cccccccccc
     7321 gacatttcgc ggtgaagaat cgtcgtcatc aaaaagcctg aaatcacaaa gaatatatcg
     7381 acaccgagaa acccgcccgg cagccaatcc ttttcgatat ggaacacgat gacggacaag
     7441 acggcggcgg cgcgcaatgt gtcgatgtcc gggcggtagg gtaaggcttg gctcataatg
     7501 tttttatagt ggattaacaa aaaccagtac ggcgttgcct cgccttgccg tactatctgt
     7561 actgtctgcg gtttcgtcgc cttgtcctga tttaaagtta atccactata ctcgaaacgc
     7621 ggcggcgcaa atgccgtctg aaaggtcatt ttgtacggga tattcggaat gccggacgga
     7681 tttttgcaac agcggggcag gcggtttgtt ttgcaggaat cggggagggc aaatcggaaa
     7741 tgcggacggg attttatttt gatgcagccg cattccggcg gtatggaaac gccgaaaacc
     7801 accaaaaccg gcttcagacg gcatttctgg caaagccgtc tgaaaacctg ccgcatttgg
     7861 gttacacgtt aaacaaaaag tgcatcacat ccccatcctg cacgacgtat tccttacctt
     7921 ccacacgcat tttgccggct tctttggctt tggcttcgcc gccgagcgag acaaagtcgt
     7981 cgtaagaaat gacttgggcg cggatgaagc cgcgttcgaa gtcggtgtga atcacgccgg
     8041 cggcttgtgg ggctgtgtcg cctttgtgta tcgtccacgc gcggacttct ttgacgccgg
     8101 cagtgaagta ggtttgcagc cccaaaaggt cgtaaccggc gcggatcagg cggttcaggc
     8161 ccggttcttc caagcccatt tcggcgagga actcggcttt ttcaccgtct tccaactcgg
     8221 cgatttcgct ctccatcgcg gcgcacacgg cgacgacggg ggcgttttct tttgccgcca
     8281 attctttcag gcggtcgagg tgcgggttgt tttcaaaacc gtcttcggaa acgttgccga
     8341 cgtacatggc aggtttggcg gtcagcagga acagcggttt gagcatcgcg cgttcttccg
     8401 cgtccaaacc gaaggaacgc acgggtttgc cttcgtccag atgcggcagc agttttttgc
     8461 acaaatcgac cagcttttgc gcgtctttgt caccggaacg ggcgcgtttt tcttcgcgga
     8521 cgatggcttt ttccacactt gccaagtcgg caagcgccaa ctctgtaccg atggtttcga
     8581 tgtcggcaat cggatcgacg cggcctgcaa cgtggacgat gttgtcgtcg tcaaagcagc
     8641 gcacgacatt cacaatcgca tcggtttcgc ggatgttggc aaggaactgg ttgcccaagc
     8701 cctcgccttt gctcgcgcct gcaaccaaac cggcaatatc gacaaattcg acgatggcag
     8761 gctgcatttt ttgcggattg acgatttttg ccaattcggc catacgcgga tcggggactt
     8821 cgacgatgcc gacgttgggt tcgatggtac agaaaggata gtttgccgct tcgatacccg
     8881 attgggtcag cgcgttaaaa agggtggatt tgccgacgtt gggcaaaccg acgatgccgc
     8941 atttcaaact catgtttttt cctgaaaata gagaaattta acggcggatt atagcatacc
     9001 gccgcccgcg ttccgaaaaa tgccgtctga aacggcttca gacagcatcc ggtttcaaaa
     9061 accgttcaga acaagccgtg aatcacgccg tgttcgtcca catcgatttt ctcggcggcc
     9121 ggaactttcg gcagacccgg catcttcatc atattgccgc acagcgcgac gatgaagccc
     9181 gcgccggcgg aaacggtgat gccgcgcacg gcgatgcgga agccttcggg gcagcccaac
     9241 agtttggcgt tgtcactcaa agagtattgg gttttcgcca tgcagatcgg cattttgtcc
     9301 aagcccagtt tttccagtga agcgatttcg gcagacgctt ccgcgctgaa atcaacatct
     9361 tccgcgccgt acactttttg ggcaatcgca cggattttgt ctttgatgcc caactcgaca
     9421 tcgtaggcga aaccgaagtt attggtttga ctttcaatgg cgttgacgac tttgcgcgcc
     9481 aaatccgcgc cgcccgcacc acctttgccc cacacttcgg tcagggaaac ttcaacgccg
     9541 tgttcggcac aggctttttc aatcatcgcc aactcggcat cggagtcgga cacgaagcgg
     9601 ttgagcgcaa cgacgacggg cagtccgaat acgtttttca ggttggaaat gtgtttcagc
     9661 aggttgggca aacctttttc caaagcgtct aaattttctt cgccgaggtt ggcgcgttcc
     9721 acgccgccgt tatatttcaa cgcgcggaca gtcgccacga caacagccgc atcaggtttc
     9781 aaaccggcaa ggcggcattt gatgtcgcag aatttttccg cgcccaagtc cgcgccgaag
     9841 cctgcttcgg ttacggcgta atcggcaagg tgtttcgcca gacgggttgc ggttacggag
     9901 ttgcagccgt gggcgatgtt ggcgaacggg ccgccgtgta cgaaggcggg cgtgccttcg
     9961 atggtttgca ccaagttagg cttaatcgca tctttgagca gggccgccat cgcgccgtgt
    10021 gccttcaaat ctttggcgta aacggggctg ccgtctttgg cgtaggcgac aaggatgttg
    10081 cccaaacgct ctttcaaatc gccgatgtct ttggcgagac agaataccgc catcacttcg
    10141 gaggcaacgg taatgtcgaa accgtcgggg cgcatcacac cgtcaacagg tttacccatg
    10201 ccgtcgatga tgttgcgcaa ctggcggtcg ttcatatcga ccacgcgccg ccacagcacg
    10261 cgtttggggt cgatgttcaa ctcgttgcct tggtagatat ggttgtcgag catggcagcg
    10321 agcaggttat tcgccgcacc gatggcgtga aagtcgccgg tgaagtgcag gttgatgtct
    10381 tccatcggca aaacttgggc atagccgccg cctgccgcgc cgcctttcac gccgaacacc
    10441 ggccccagag aaggttcgcg cagggcaatc acggcatctt tgccgatgtg gcgcaacgcg
    10501 tccgccaaac cgatggttac ggtggttttg ccttcgcccg ccggagtcgg gttgatggcg
    10561 gtaaccaaaa tcagcctgcc ctgtttttgc agcagtttga acgcttcggc aggattgatt
    10621 ttcgccttgt aatgaccgta aggctcaatg ttgtcggcgt tcagacccag cttggcggca
    10681 atttcgccaa tcgggcgcat cgtggaagac tgggcgattt cggcatcggt tttgaagctc
    10741 atgattttcc tttagaaatg aggagggaca tactgtctga aagcatcagg cgacaaacag
    10801 gtggattgaa aataatatca ggcatattat aacgttatcc gcaccaaacc cgcagtgaaa
    10861 tttttgacgc tgcaacaaaa ataccgttca tattgttcac aatccaagga gaaaacatgg
    10921 gcagcaacgc atggctgttt tgggcattgg catcggcagg cttcgcctca ttgaccgcca
    10981 ttttcgccaa aatgggttta cagggcatag attcggattt cgccaccttt atccgcacct
    11041 tggtcatcct cgccgctttg gtattgtttt taacctacac cggcaaatgg cagggcgtga
    11101 acggctttac cggcctcaac tggacattcc tcatcctatc cggcctcgct accggcgcat
    11161 cttggctcgc ctacttcaaa gccctgcaac tgggtaacgc ctcgcaagtc gcccccgtcg
    11221 ataaattcag cctggttctg gtcgccctga tggcagtagt cttcctcaac gaacgcccca
    11281 gcacgcagga atggataggc ttggggctgg taacggcggg cgtgttggtg ctggcgttga
    11341 aacgttaaac cgaatccgcc ataccgcctg aaaccgggtt ttgacaatct gacgggcggc
    11401 ggtctgaaca aagttaccca ataaaatccg acaccgcgcc gacggcaaac cgccgcatcc
    11461 gacaggggtc ggcttcagat ggcatttgtt ggacggaaag ccccgaacgc cctattttca
    11521 cgcctccacg aaaatatgct ggacatccgg tcggaaccgt tcggcagccg gctcggcaag
    11581 ccaccgtttt atttcacttc caagcccctg caagggcttg agcctctttc agacggcata
    11641 ccgtgccgac atccagccac aagcccgtat gcttctgacc gctcacgcgg ttttgccgca
    11701 tttcgccacg caatacgggc gcgagtttcg ccacactgcc cgcttcgatt ccgtcaaaca
    11761 tttcaggacg gtaaataccc acgccgctga atgtcaatcc gttgccgcca tttacttccg
    11821 gccgcacgct gccgtcgggc agcagggaaa aatcgccgtc ggggttgtgc ggcggatttt
    11881 ccaccagcca cagatgggcg gaaatatgtt ccggcaggga cgatgccgcc tgaaacgcgg
    11941 cggtaaaatc gatgtcggtc agcacgtcgc cgttgaccac caaaaacggc tgcccaccca
    12001 acagcggcaa tgcctgcgcg atgccgcctg ccgtttccaa accgcctgcg ggttcgggcg
    12061 aataggcgat gttcacgcca taagccgagc cgtcgcccaa agcatcttct atctgccgac
    12121 ccagccaagc gtggttgatg acgatttcgg taaaccccgc ctgcttcaga cggcataggt
    12181 gccaaccgat tagaggctta cccgccacat cgagcagcgg cttcggagtg gtatcggtca
    12241 aagggcgcat acgctcgccg cgtcctgccg ccagtatcat cgctttcata tatctgtccg
    12301 aatatcagtc taaaaatcta aactgccgtc tgaaatacag cagcgcgggg cgtttgcacc
    12361 cgcagttttt gatttcgtcg agcctgacgt aaaacacaaa atgcgtgccg atttcatgtt
    12421 tgccgacaat atgcccgtgc aggtgcgcca acgcgccttc tatttcaggt tgtccggttt
    12481 taccgtaatg ccaaatatga taggcaaaac gttcttcggg tgaaagattg gtcaatccgg
    12541 caaaatgttc ggccacatcc tgatgttcgt ctgccaacgt attgatgcac aggctgccgt
    12601 tttccgacag tatcgggatg atacgggctt tacggttgat gcacagcatc acggtcggcg
    12661 gctcgtccgt taccggcgcg accgccgtca ttgtaatgcc gtaacgccct gccgcaccgt
    12721 ctgtcgtgat gacatgaacg cctgccgcac aggatgccat cgcatcacgg aacgaagttt
    12781 gaaaagtttt ctgcaaatcc gccatttttc ccctttaaac tgtcccctat ataagaatgc
    12841 tgcacacaag gcacccccca tgtgcagcag ttctgattca aaaagccgtc ggtcggacat
    12901 ttccgcgcgt tacggcgtat tacgagttca acgcatcctc gattttggca agttctgcca
    12961 acaggtcttt aagcagcagc attttctcgc ggcccagcac ttcctcgata gcgtcgtaac
    13021 gctcgtccac ttcttcgccg atttcctcat acagcttctc gccctcggca gtcagcttca
    13081 gaaaaacacg tcgttggtcg ttggaaggtt tcaggcggac aaccaaaccc gctttttcaa
    13141 ggcgggtcag gataccggtc aggctggggc gcagaataca cgcctgattc gccaaatctt
    13201 gaaagtccag cgtgccgttt tccgccaaaa gacggataat ccgccattgc tgatcggtaa
    13261 tattcgcctg attcagaata ggcctgaatt gggtcatcag ggcttccctt gcctgtatca
    13321 gactgatatt gatagacgca tgttttgatt gggtaggcat tgttcaagtc tccaagttat
    13381 cgaaaatcaa actttcaaac cgtcgggaaa gcctgtgggc gtaatttttg atgcaaccgt
    13441 tatataacaa aacgaacata tagcaacaat acgctataaa ccgcatcgga cgactgggta
    13501 taaaagactt taattccgat aatcctatct aaaaatattt taatagttat atcttagtct
    13561 attttcccta caatctcaac aaagtattac atttactgtc gaaagaactt tcttccagaa
    13621 aaaacaaaag ccgccgcatc agacgagcaa ggtatatgcc gtttgattct ccacatagcg
    13681 taaagtttaa caaaaacaaa agttaaccga aaacatccgc ctgaaaaatt cgtgcgcgca
    13741 agccccaata actgctgatt cccgtcgtat agtgaaccat tttcccattt ttgaccaaaa
    13801 cgacggcagg cgttgcgaca atccgccaag accttgccaa acccccgtcc tcatcgttga
    13861 ccgtcggaaa gcccaagccc cgctttgcca tatacgccgc cacttcggac gcgctgccgg
    13921 aacgtaccgc cacgccgacg accggaacgc cgtccgccgc caaatcatcg attatcggcg
    13981 actgataacg gcacacgccg caccagcttc cccaaaaata caccaaaatc gccttatccc
    14041 ggctaaactg tcccaaagtc agccgctgcc ccgacagcag ggtcaaaggc cgccctgccg
    14101 caccggccgg ctcttcgggc ttgcgtatcc aatccaaaaa cagcgacacc aataaaaaca
    14161 ccaatgccgt ctgaacggca aatttgatgc ccgaaagcag tttctttttc atacgctctc
    14221 tcaaacggta cgcccgcgca accggcaggc aaacaaaaag ccaagtctca aaacttggct
    14281 tccggttatc tggtgggtcg tgagcgattc gaacgctcga ccaacggatt aaaagtccgc
    14341 tgctctaccg gctgagctaa cgacccgata agccgtgcat tatacagcac catcctacct
    14401 cgtcaagcaa attttacagg cttaattgca gaccactgtt tgcacgggat attttgacaa
    14461 cggattttca caatccgccg cataccgtgt aaaagttcgc acaaggaaag gcaaaccgcc
    14521 cgaaatcaat gtacactttc cgcccgtttc ccaaactgca cacagaaaca cacattatga
    14581 acatacaaaa catccgcacc ctcctcgaca ccgtcgccgt tccgaatacg gcacgcacgc
    14641 tcggcggcga aaaggccgtc cgttcggtcg aacagcgttc agacggcatc catatcgccc
    14701 tgcatttcgg cttccccgtc gcgcacattg cctcagaaac agccgaccgc atacaggaaa
    14761 tcctgatgcc cgaaacaggc gacacacaca tccatctgtc catgaacacg gaaatcggca
    14821 cacacaaagt ccagcccggc gttaccacta tcaaaggtgt gaaaaacatc attgccgtcg
    14881 catcgggaaa aggcggcgtg ggcaaatcaa ccaccaccgc caaccttgcc gccgcaatgg
    14941 cgcggatggg cgcgcgcgtc ggcgtgctcg atgccgacct ttacggcccg agccaaccga
    15001 ccatgttggg tgtggacgac cgcaaacccg atcagaaaaa ccaaaaactc attcccgtcg
    15061 aatcttcaga cggcatacag gtcatgtcta tcggctttct cgtcgatacc gaccaagccg
    15121 tcgtctggcg cgggccgatg gtcagccaag ccttgcagca actgatgttt caaagcgagt
    15181 gggacgaagt ggactacctg tttatcgacc tgccccccgg cacgggcgac atccaactca
    15241 cgctgtccca acgcatcccc gtaaccggat cggtcatcgt aaccacgccg caggacatcg
    15301 ccctgataga cgcgcgcaaa gccgtggata tgttccgaaa agtcaacatt cccattttag
    15361 gtgtattgga aaatatgtcc gtccacatct gctccaactg cggacacagc gaagcactgt
    15421 tcggcacaga cggcggcaaa gatttagccg cacgcctcaa cgtccccctg ctcggacagc
    15481 ttcccctaag cctgcccgtg cgcgaagcca tggacggcgg cacaccggcg caactgttcg
    15541 acgaacaccc cgccatcgcc cgaatctaca ccgatgccgc attccaaatc gccctgggca
    15601 ttgccgacaa aggcaaagac ttcagcagcc ggttccctaa aatcgtcgtc gaataaagcc
    15661 gcgtccgaaa ccgcaacagc aatgccgtcc caagccccgc gcctgccggc gggcttcata
    15721 gtcctctctc cccgtgggag agggctggag agagggcaac aagccgcaag gcttgtattg
    15781 gggcggttaa gctgttggga aagattgccg caacttggag aatgccctct ccccggccct
    15841 cccccacagg ggagggagcg gattgcggca ggttttgcgg ttgcaggcgg tttgaaaaac
    15901 agattggatt tacaaccgtt gatgcaggtc gtctgaaaag caaaaagcag cctgcacaac
    15961 ctgattagtt tacaaaaccc ttaaccccaa cagcctcccc gtcctctctc cccgtgggag
    16021 agggctggag agagggcaac aagccgaaag gcttgtattg gggcggttga gttgttggga
    16081 aaggttgccg caacttggaa aatgccgcct ccccggccct cccccacggg ggagggagtg
    16141 ggtttcggca gatttttcgg ttgcaggcga tttgaaaggc aacttagatt tgcagctgtt
    16201 gtttcaggtc gtctgaaaaa taaaaagcag cctgcacaac ctgttttcct tgcagcaaac
    16261 ctcaacccca acagccgacc cgtcctctct ccctgtggga gagagctaga gagagggcaa
    16321 caagccgaaa ggcttgtatt ggggcggttg agttgttggg aaagattgcc gcaacttgaa
    16381 aaatgccgcc tccccggccc gccattgccg cgcaggcggc aatggcgggc ctgccggcgg
    16441 gcaaacttgc cggataaaac ggtttttttg agattttacg ttccggattc ccgctttcgc
    16501 ggcaatggcg ggccaaggag gcggttgcgg attgccgaaa accgctttac catgatcagc
    16561 gcgcaagggt agaataatgc ggtaacccta tacattgcat ccggccaaag gggcgtgtta
    16621 cttttccgaa catccccctt tggcagccgg gccaaagggg ttttccaacc agcaattcgg
    16681 cgggcgcgga atcgggcggt ttgccgaatc ccgccgtttg cggcgcgcct gccgccgacg
    16741 gtatcccgcg aagcaagatt taagggataa aatatgttcc aacacgcagg gcggcacata
    16801 aggcgccgcc ctgattcgga agggcttgca cccctcccgg acaaagcctg atcctgccgc
    16861 cccgaaggac ggatgcccga agggcggggg gtttgaccga aaaggaaata cgatgaataa
    16921 aactttaaaa aggcaggttt tccgccatac cgcgctttat gccgccatct tgatgttttc
    16981 ccataccggc gggggggggg gcgcgcgcag gcggaaaccc gtaactacgc tattgtcatg
    17041 aacaaacaaa atcagccgga ggtaaagcgg gagggttcat attcaacctt aagggaaaaa
    17101 gacaggaaac gcaaatttga ctttaatgcg aacaacgggg gtggaggctc tgtctcattc
    17161 aacaatagcg atgagcttgt ttctcgacaa aacggtactg ccgtttttgg cacagccacc
    17221 tacctgccgc cctacggcaa ggtttccggt tttgatgaca aaaggctgaa agagcgcggc
    17281 aatgccgtta attggattca tacgacccac ccagggttga taggctacag ctacgccggt
    17341 gtcgtatgca gaaacagcac aggctgtccc caacttgtct ataaaacccg attttccttc
    17401 gacaacaccg gtttggcaaa aaatgcgggc atgctggata gtcacccgga cccaagccgc
    17461 gaaaattcgc ccatttacaa attgaaagat tatccatggt tgggcgtatc tttcaatttg
    17521 ggcagcgaga ataccgtcaa aaatagccaa tcatccaaca gattgatatc ttcttttagt
    17581 gaaaacaata ataatcaaac catcgtctct acgacagaaa gccaccctat ttcccttggc
    17641 gacgggcagc gcgaacatac cgccgtggtc tattatctga acgccaagct gcacctgctg
    17701 aataaaaaac agattcaaaa tatcaccgac aaaacagtgc agttgggtgt cttgaagccg
    17761 agcatcgatg tgaagacaca aaggacgggg tttggcggca ttctatcttt ttgggctagt
    17821 tgggacatta aagataccgg gcagattcca gtcaagctcg gcctgcagca agtcaaagca
    17881 ggccgctgcg tcaacgcaaa taaccccaat aagagtacca aagccccttc ccccgccctg
    17941 actgcccccg cgctgtggtt cggacctgtg caaaatggta aggtg
//
LOCUS       DAWHRS010000024        17604 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWHRS010000024 DAWHRS010000000
VERSION     DAWHRS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17604
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.024"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(31..672)
                     /locus_tag="WIB82_000572"
     CDS             complement(31..672)
                     /locus_tag="WIB82_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ3841911.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(662..1213)
                     /locus_tag="WIB82_000573"
     CDS             complement(662..1213)
                     /locus_tag="WIB82_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ3841912.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIDSDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(1217..1735)
                     /locus_tag="WIB82_000574"
     CDS             complement(1217..1735)
                     /locus_tag="WIB82_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ3841913.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDNTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(1735..3396)
                     /gene="pqiB"
                     /locus_tag="WIB82_000575"
     CDS             complement(1735..3396)
                     /gene="pqiB"
                     /locus_tag="WIB82_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ3841914.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATRGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLAGNPQTQNIPNELNQTLKELRQTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(3389..4762)
                     /locus_tag="WIB82_000576"
     CDS             complement(3389..4762)
                     /locus_tag="WIB82_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ3841915.1"
                     /translation="MHPDWVYRLFSFIMPPLIFATFPMKPFAENIPHSLRGNCCDEAL
                     PPHTVDCPECGCRADVPRLDSGEAAFCPRCGHKLFRVGRHPFSAPPAYAAASLILMAF
                     AYGMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVY
                     AALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMF
                     ALSVMLIRTSVSVPQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDGAESPCGVC
                     GAELYRRRPKSLSISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDE
                     GDRLIAAVIFSASILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMI
                     DIFVIIILMCSFHTYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAF
                     NETEKHD"
     gene            5186..5815
                     /locus_tag="WIB82_000577"
     CDS             5186..5815
                     /locus_tag="WIB82_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ3841916.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGACEAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            5990..8365
                     /gene="hmbR"
                     /locus_tag="WIB82_000578"
     CDS             5990..8365
                     /gene="hmbR"
                     /locus_tag="WIB82_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HEZ3841917.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVLAADEAATETTPVKAEIKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIEIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLDDRQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGNRGYAVEGEGSGANIRGSARGIPDSSKHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLTASSWREADDVNRRRNANLFYEWMPDSNWLSSLKADFDYQKTKV
                     AAVNNKGSFPMDYSTWTRNYNQKDLDEIYNRSMDTRFKRFTLRLDSHPLQLGGGRHRL
                     SFKTFVSRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSSR
                     AGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWRVGYDITSG
                     YRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGMLDANLYQSNYRN
                     FLSEEQKLTTSGTPGCTEENAYYGICSDPYKEKLDWQMKNIDKARIRGIELTGRLNVD
                     KVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLTY
                     LGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPAKNLTLRAGV
                     YNLFNRKYTTWDSLRGLYSYSTTNAVDRDGKGLDRYRAPGRNYAVSLEWKF"
     gene            complement(8573..8695)
                     /locus_tag="WIB82_000579"
                     /pseudo
     CDS             complement(8573..8695)
                     /locus_tag="WIB82_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
     gene            9088..9336
                     /locus_tag="WIB82_000580"
     CDS             9088..9336
                     /locus_tag="WIB82_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ3841918.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
     gene            9333..9719
                     /locus_tag="WIB82_000581"
     CDS             9333..9719
                     /locus_tag="WIB82_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ3841919.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(9899..11254)
                     /gene="yegQ"
                     /locus_tag="WIB82_000582"
     CDS             complement(9899..11254)
                     /gene="yegQ"
                     /locus_tag="WIB82_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ3841920.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVSDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            11579..12367
                     /locus_tag="WIB82_000583"
     CDS             11579..12367
                     /locus_tag="WIB82_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ3841921.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAEAGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGWEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            complement(12413..13012)
                     /locus_tag="WIB82_000584"
     CDS             complement(12413..13012)
                     /locus_tag="WIB82_000584"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ3841922.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKLGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            13140..13757
                     /gene="gmk"
                     /locus_tag="WIB82_000585"
     CDS             13140..13757
                     /gene="gmk"
                     /locus_tag="WIB82_000585"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ3841923.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            13816..14022
                     /locus_tag="WIB82_000586"
     CDS             13816..14022
                     /locus_tag="WIB82_000586"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ3841924.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            14115..16271
                     /locus_tag="WIB82_000587"
     CDS             14115..16271
                     /locus_tag="WIB82_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ3841925.1"
                     /translation="MPVPQPTAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(16338..17522)
                     /gene="coaBC"
                     /locus_tag="WIB82_000588"
     CDS             complement(16338..17522)
                     /gene="coaBC"
                     /locus_tag="WIB82_000588"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ3841926.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILKGKKVLITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAENMHRAVHRLIDKQDAFISVAAVSD
                     YRVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMTYAR
                     EKRIKKKLPMIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMRIVERLAVYL
                     SK"
BASE COUNT         4370 a         4246 c         4719 g         4269 t
ORIGIN      
        1 taaacaactt gggtgcggat aaacctttgt ttatctaaac ccctgttttc tcaaaaattg
       61 attcaaatct tcggcaaatt tccgatagcc tttgccgttg gcgtggattt ggtcggattt
      121 cagattatta tcgcccaaaa tttccgccca cgcgccgccg aacagcggaa tgccgtattc
      181 ctcggacaaa tcctcataca gcggatgatc gctcaaatgc ccgaacaacg cacccagtgt
      241 gatgtgcggc acgccgacga ggacggcggg gatgttttcc ttctgcacgg tttcgatgat
      301 tttcgcgata ttggcgcggg tctgctcctt gggaactttg cgcagaaagt cgttgccgcc
      361 tatgccgaca atcacaagct tgggtttgcg tgccaacagc gcgggcaggc gcgacagggc
      421 ttgggcagat gtatcgcccg atacgccgcc gttgacaata ttccaacccg tcagtttttg
      481 cagttgcgcg gggtaggatt cgccagggtt tgcgccgtag ccgaaggtaa gcgaatcgcc
      541 caaggcaagt acggtgcttc cttcgggaat tttggcgtgg gttcgggcgg attttctgcc
      601 gcaggcggta agcagcaacg cgcctgcgcc gaggaggaag gttcttctgt tcattggttt
      661 ttcagatggc atcgagccat ttcgggtggt atttcagggt ttcacggtaa accggacagc
      721 tttcggcgtg cgcgcctttg aggtagccgg tactcatcag aaattcgccg acgatttcgc
      781 cgccgacgaa tttgaaatgt tttttaaaga gtttaaccca ttcgtctttg cttcgcggat
      841 ggtgcgcgtc gagccagttc ttgaacgagc cgtattcttg ttgcaacgct tggatttgcc
      901 gtgcattgaa aatggcggca tcgattttca ggcggttgcg gacgatgccc gcgtcggcaa
      961 gcaggcgttc gcggtcggtg tcgtcgaagg cggcaaccgt atcgatgtcg aaaccttcaa
     1021 atgccgtctg aaacgcctgc cgctttttca gcatcagcgt ccagcttaat cctgcctgat
     1081 tgatttccaa caccagccgt tcaaacagtc cgttgtcgct gtcaatcgga aaaccgtatt
     1141 gcgtgtcgtg gtaatgtttg ttcgggttat cggtgttttc gggaagtgag gcggcaaatt
     1201 cgcaatagtt cacggtttac tcgaccatct gttgcgccgc ctgtttcagt ccctgttcga
     1261 gtgcggcggt catcgcggcg tagccgtcac cctgctgttc ggtttcgata tggaagggtc
     1321 tgttcgtacc gtcgggtagg acggcgtagc cgctgatgag ggttttgccc gtgtagctgc
     1381 cttggaatgc gtcgatatag accgtccatt tttcggtact gccgctgcgt gaggcaggaa
     1441 caaagatgcg tgtgttgtcc aaacggttga atgcattgct caacgccgct tcgagcatat
     1501 cgtccaaggt gtctgcccaa acgtggtttt gtgcggtgtt gaggcggtag gggtcggttt
     1561 gatagaccag tccgccgcgt ttgagcggtt cggcaagacg gacttcgacg gcggtttcgc
     1621 cgccttgtgt tgcaggacgg atgtagcggc tgtcgggcaa cacgaaatat tgtgtgcttt
     1681 gcacagtacc gcaggcggca agcgtcaggg cggcggcaat cgggaagagg cgcattatcg
     1741 gcttcctttc gggatggggt ctttgctgct gctgttgaaa atcagcgcgt tgggtttttc
     1801 tttcaaagtg ttaatgacgg gttgaacgtc ttttaaggtt ttgtccaaac tttgcaacgt
     1861 attttgtacg tcgccgtaga taggcgattg aggcgatacg ccttgcaggg tttgacgtaa
     1921 ttctttcagg gtttggttca gttcgttcgg aatgttttgc gtctgcggat tgccggccag
     1981 tttgtcaatg gagcttaggg cggcattggc agatttgagt gcggacttga gctcggcaag
     2041 cgaaccgttc aattcggcaa ccgtcttatc caaaggcagt ttgtcgaact tgtccagcaa
     2101 atccaccact ttcgcctgca aatcgtccaa accgccgccc cgtgtggcga tgacggtatc
     2161 gcctgcataa acggtatgcg gtcgcagctt gggcgaagac gaaggctgat cgttcaactc
     2221 aatcattttg ctgccggtca gcaggttgtt gctggagatg gtggcggtca ggcctttgtt
     2281 taaagccgtc tgaaattgtt gtttccaatg ctctttgctt tgttcgtcgg cattgatttc
     2341 caaacgggaa ggctcgatgc ggatgcgtac gggaatccag ccgttttcaa ataaatgcag
     2401 gctgtcgttg cggtcgaaat aagggacatc ggaaaccacg ccgacattca gccctttgta
     2461 ctcgacgggc gaaccgacgg tcagtccgcg cacggattgt ttgaaaaacg cggtgtagta
     2521 cagcgagcgg tcgtcaggca ggttggcgac ttcgctgcgg ctgtcgtaaa gcgtgaagct
     2581 gtcttcgctt ttaacgtttt tactgttttt ggttttcggc gaatcaaatg aaatcgcgcc
     2641 cgacagcagg gcaggcagag gggcggaatt gagtttgatg ccgctgcctg tggtttcgat
     2701 attgatgccg ctttccagcc aaaaacggct ggcggaatga atcagtttgt cgttggggct
     2761 ttggatgaag atggtgtaat gcacgctttg gtcggacggg tcgaaatgcg cgctttcgac
     2821 ttgcccgacc ataaagtttt catacaaaac agggctgttg acgttgagga tgcggtcgtt
     2881 tttaccaatc aaattcaagc gcagcccgct ttgcccgatg gcggtaacgg gcggaatgtc
     2941 ctgcacttgg aacacgtctt ttgcctcgtc gcttttgccg ggtgtaaaag cgatgtacga
     3001 acccgaaagc agcgtaccca aaccggttac gccgctttgg tcgatacgcg gcttgaccac
     3061 ccaaaactgt gtgtcgctgc ggatgaggcc ggatacgtcc gcattgagtt gggcggttac
     3121 ttccacgcct ttctggtcgt cgcgcagttt gattcgggta acgcgtccga catcgatgct
     3181 caatacctta atgaccgtat tgttgacctc aatgccttcc gcgctgtcca tcaagagcgt
     3241 aaccacaggc cccctgttgc ggatttcctt aatccacagc cagccaccgg caatcagcgc
     3301 aatcagcggg accagccaga cggcggagag caaggtgttg tttttgcgga cgcgtgcttg
     3361 ggcgtgtccg tttggaggag ggctgttgtc agtcatgttt ttccgtttca ttgaaagcaa
     3421 tgccgtctga agcgcgtttg tcccaaagca ggcgcgggtc gaaataatag gcggacagca
     3481 tcgtcagaat cacgaccagg cagaaataga ctgccgcact gcccggaatg acgcgcgcgg
     3541 cataagtgtg gaacgaacac atcaaaataa taatcacaaa aatatcaatc atcgaccagc
     3601 ggccgaccgc ttcggtgatg cggtagaggt gcgacaattt ctttgcgccc gttggcaaag
     3661 cgaagcgggc ggacgcaatc aaaaccgaca ttgccgcaat cttcagtacc ggcaccaaaa
     3721 tactcgcgct gaaaataacc gccgcaatca gcctgtcgcc ctcgtcccac atataagcga
     3781 tgccgttaag gatggtattg acctccgtgg cggcaggatt ggacgaaatc ataatcggca
     3841 ggatattggc agggaaatac aaaataaccg ccgccgtcag aaacgccgac gaaatactca
     3901 gacttttcgg ccgtcggcgg tacagttccg caccgcacac gccgcagggg gattcggcac
     3961 cgtctcggaa atacaggcag cggctgcaac aggttttgcc ttccgatgcc gtctgaaccg
     4021 cattatcccc cgtcagccgc ccgatttgaa aatataccca atgctgggga acggataccg
     4081 aagtccgaat cagcataacc gacagcgcga acatcagata aaacgccggc ccgaagcgaa
     4141 cctctgccac agacgagagc ttgatatacg ccaccaaagt ggaaacaaaa aacacatcca
     4201 ccatcatcgc ctgcctcaag cgcaccatca cacgcgttgc caaacgcagc gcaggatacg
     4261 cctgtttccg tatcagcgcg gcatagacat acaggcacag cagcagaaac agaaccggcg
     4321 cgccgaaggt cagcacaaac atcacttcgg ccaaaaaacc ataatcctga aacaccatca
     4381 ggcgcatcat ctcgggcagc gaaaggacgg atgccgcacc cggtatcccg acctcgatat
     4441 acgtcatacc gtaagcaaac gccatcaaaa tcagcgaagc cgccgcataa gcgggcgggg
     4501 cggaaaaagg atgcctgccc accctgaaga gtttgtgtcc gcaacgggga cagaacgccg
     4561 cttctccact gtccaaccgg ggtacatccg cgcggcagcc gcattccgga caatctaccg
     4621 tatgcggcgg cagggcttcg tcgcagcagt tgccgcgaag gctgtggggg atattttcgg
     4681 caaacggctt catcgggaaa gtggcaaata taagtggcgg cattataaag gaaaaaaggc
     4741 gatacaccca atcaggatgc aaaaccggca ggttcggcag tatgaacaaa cccttggtgt
     4801 ccggcaaaaa tattttttga gcggttgtcc gccgactgcc gaacccattt gataaaatcc
     4861 cgattctgta aagaatagta aaatacctgc ttaaaaacat cacgcaaatg atattttttc
     4921 caaaaatatg tgataataaa tttcgtttat gattctttat tatttttata gtggattaac
     4981 aaaaatcagg acaaggcggc gaagccgcag acagtacaaa tagtacggaa ccgactcact
     5041 tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca acgccgtact
     5101 ggtttttgtt aatccactat atatgcgggc aagtgcggat tttgcccgca ccgcatcctt
     5161 atcccttacc gacaaaggaa aaaatatgag tgaaaccgaa aatcaagcat tgacatttgc
     5221 caaacgcttg aaggcggata ccacggcggt tcacgacagc gtggataacc tcgttatgtc
     5281 tgtccaaccg tttgtcagca aagaaaacta catcaaattt ttgaaactcc aatccgtttt
     5341 ccacaaggct gtcgaccaca tctataaaga tgccgaatta aacaaagcca ttcccgagct
     5401 ggaatacatg gcgcgatacg atgccgtaac gcaagacctt gcagatttgg gtgacaaacc
     5461 ttacgaatac ggcaaaccgc tgccgcatga aaccggcaac aaagcaatcg gctggcttta
     5521 ttgcgccgaa ggatccaatt tgggcgcggc atttttgttc aaacacgccc aaaaactcga
     5581 ttacaacggc gaacacggcg cgcgccacct cgcaccccat cccgacgggc gcggcaaaca
     5641 ctggcgcgct ttcgtcgagc atctgaacgc tttgaacctg actcccgaag ccgaagcgga
     5701 agccatccaa ggcgcgtgcg aagcctttgc attctacaaa gtcgtgttgc gcgaaacctt
     5761 cggcttggca gccgatgccg aagcaccgga aggaatgatg ccgcacaggc actaaaaaat
     5821 aatcgaacca aataaacaag gtctcggcat agctgtttgc agggaccttt aattacacgg
     5881 cgcggctttg tttacatgga ttactgtctt attaaatatt aatgattatc ataaaatcta
     5941 ttattcgcta accgatggat gaacaatcca tacatcttga gttgataata tgaaaccatt
     6001 acaaatgctc cctatcgccg cgctggtcgg cagtattttc ggcaatccgg tcttggcagc
     6061 agatgaagct gcaactgaaa ccacacccgt taaggcagag ataaaagcag tgcgcgttaa
     6121 aggtcagcgc aatgcgcctg cggctgtgga acgcgtcaac cttaaccgta tcaaacaaga
     6181 aatgatacgc gacaataaag acttggtgcg ctattccacc gatgtcggct tgagcgacag
     6241 cggccgccat caaaaaggct ttgctgttcg cggcgtggaa ggcaaccgtg tcggcgtgag
     6301 catagacggt gtaaacctgc ctgattctga agaaaactcg ctgtacgccc gttatggcaa
     6361 cttcaacagc tcgcgtttgt ctatcgaccc cgaactcgtg cgcaacatcg aaatcgtgaa
     6421 gggcgcagac tctttcaata ccggcagtgg tgcattgggc ggcggtgtga attaccaaac
     6481 gctgcaaggc cgtgatttgc tgttggacga caggcaattc ggcgtgatga tgaaaaacgg
     6541 ttacagcacg cgtaaccgtg aatggacaaa tactctcggt ttcggtgtga gtaacgaccg
     6601 cgtggatgct gctttgctgt attcgcaacg tcgcggtcat gaaaccgaaa gtgcgggaaa
     6661 ccgaggctat gctgtggaag gggaaggcag tggcgcgaat atccgtggtt cggcacgcgg
     6721 tatccctgat tcgtccaaac acaaatacca cagctttttg ggtaagattg cttaccaaat
     6781 taacgataac caccgcatcg gcgcatcgct taacggccag cagggacata attacacggt
     6841 tgaagagtct tataacctga ccgcttcttc ctggcgcgaa gccgatgacg taaacagacg
     6901 gcgcaatgcc aacctctttt acgaatggat gcctgattca aattggttgt cgtctttgaa
     6961 ggcggacttc gattatcaga aaaccaaagt ggcggcggtt aacaacaaag gctcgttccc
     7021 gatggattat tccacctgga cgcgcaacta taatcagaag gatttggacg aaatatacaa
     7081 ccgcagcatg gacacccgat tcaaacgttt tactttgcgt ttggacagcc atccgttgca
     7141 actcgggggg gggcgacacc gcctgtcgtt taaaactttc gtcagccgcc gtgattttga
     7201 aaacctaaac cgcgacgatt attacttcag cggccgtgtt gttcgaacca ccagcagtat
     7261 ccagcatccg gtgaaaacca ccaactacgg tttctcactg tctgaccaaa ttcaatggaa
     7321 cgacgtgttc agtagccgcg caggtatccg ttacgaccac accaaaatga cgcctcagga
     7381 attgaatgcc gagtgtcatg cttgtgacaa aacaccacct gcagccaaca cttataaagg
     7441 ctggagcggt tttgtcggct tggcggcgca actgaatcag gcttggcgtg tcggttacga
     7501 cattacttcc ggctaccgtg tccccaatgc gtccgaagtg tatttcactt acaaccacgg
     7561 ttcgggtaat tggctgccca atcccaacct gaaagccgag cgcagcacca cccacaccct
     7621 gtctctgcaa ggccgcagcg aaaaaggcat gctggatgcc aacctgtatc aaagcaatta
     7681 ccgcaatttc ctgtctgaag agcagaagct gaccaccagc ggcactcccg gctgtactga
     7741 ggaaaatgct tactacggta tatgcagcga cccctacaaa gaaaaactgg attggcagat
     7801 gaaaaatatc gacaaggcca gaatccgcgg tatcgagctg acaggccgtc tgaatgtgga
     7861 caaagtagcg tcttttgttc ctgagggttg gaaactgttc ggctcgctgg gttatgcgaa
     7921 aagcaaactg tcgggcgaca acagcctgct gtccacacag ccgctgaaag tgattgccgg
     7981 tatcgactat gaaagtccga gcgaaaaatg gggcgtattc tcccgcctga cctatctagg
     8041 cgcgaaaaag gtcaaagacg cgcaatacac cgtttatgaa aacaagggct ggggtacgcc
     8101 tttgcagaaa aaggtaaaag attacccgtg gctgaacaag tcggcttatg tgtttgatat
     8161 gtacggcttc tacaaaccgg ctaaaaacct gactttgcgt gcaggcgtgt acaacctgtt
     8221 caaccgcaaa tacaccactt gggattccct gcgcggttta tatagctaca gcaccaccaa
     8281 tgcggtcgac cgcgatggca aaggcttaga ccgctaccgc gccccaggcc gcaattacgc
     8341 cgtatcgctg gaatggaagt tttaatctgg tattattgaa ttaatcgcct tgttgaaaat
     8401 taaagccgtc cgaattgtgt tcaagaattc attcggacgg ctttttaccg taaatctgtg
     8461 tgatggggtt ttatattgat acagtatccg aactcaggca gggggtaatg ttgcaggatt
     8521 aaaaatgccg tctgaaaaat gtttcagacg gcattttttg gttgccgata tgttaacgga
     8581 cgacgttggc gcgcatgacc acgcggacgt ggaacgagcc tgcctgacag tcgtattgtc
     8641 cgccttgcag cggctgcttg tcgaagtagc tgtggaagcc ggtaacgccg cgtccgcaag
     8701 ggtggcggca gatgccgcat tgtgtttata atgccgtgtc agacgtttga tttatgggaa
     8761 aataatgatg cttaccaatc aggaagtaac cggtgtcaaa ctgggatacc gcaaagccgt
     8821 tttatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt
     8881 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
     8941 gaggcaacgc cgtactggtt tttgttaatc cactataaat agcaaggtgt gaataaaacg
     9001 gcggatataa tcaaatatat gtaatgagat tcccaaaatc ttggtaatga tgtatattag
     9061 tcaagttagc taatcatgag aagtgttatg tttcaagcaa atattcatca ggcaaaaacc
     9121 aatttgagtc aattgattca aagagcagaa gcaggggaaa tcgttattat tgcgaaggca
     9181 ggtaagcctt gcgtccaact aatcggtatt gaaaaaccgg cacgaaatgc aggaaggttg
     9241 aaaaaattca gtcatatgga aaatacggat atttcacgta ttcttgagga tgacaatgaa
     9301 acggcagctt tattttttga ggagtcggct ctgtgagaaa gattctgctt gatacccatg
     9361 cgttgctgtg gtggttgttg gatgacaaga aactggggat atctgcacgc aaactgatag
     9421 aaaatccgag aaatgcgatc tttgtcagtg cagcaagtat ttgggaaatt tccatcaagc
     9481 agaacaaggg gttgttgaaa ttaccggaag agttttttga tgtgcttcaa gaagaggatt
     9541 ttgaaatgtt gcctataggt ctgtttcatg caaaacaggc tggaagcctt ccggagatac
     9601 acaaagaccc tttcgatagg atgttgattg cacaaaccca agcggaaggc tttgaactga
     9661 tgactgtcga tgaatatatt ccgcaatatg gaattagagt tgtcaacgca tcaagctgac
     9721 aaatacaaaa actgtgtaca caagaaagac cgccgaggcg gtcttttcgg ttattgtctt
     9781 cgcttaaaag ccagacttct gtcgtggact ataggtgtgt cccgatctga atctgccgca
     9841 caataaggca aggggattaa aaatgccgtc tgaaaaatgt ttcagacggc ataatggctt
     9901 aggggttcaa cacgcgtgcg atgagggctt tttctttacc ctgcatattg gggattttga
     9961 cctgaatgcc gttgcccggg gcaacatcga caggctggcc tttgcgggtc atttgttcca
    10021 atttgatggt ttggttgccg ctcgggtgga tgatttcgag tgaatcgctg acggcaaagc
    10081 ggtttttgac ttccactgtt gcccagccgt tttcatcgat ttcggtaacg tgtccgacgt
    10141 attggctttg tttggcggtg gaatggccgg tcaggtagtt ttgataatcc tgagtttggt
    10201 ggcgttcgag gaagccgctg gtgtagccgc ggttggcgag gccttcgagt tcgctcaaca
    10261 ggctgtaatc aaacggacgg cctgcgacgg catcatcaat cgctttgcgg taggactggg
    10321 cgacgcgtgc aacataatag agcgatttgg tacggccttc gactttgagg ctgtccacgc
    10381 cgattttggc gagtttttcg acgacttcga taccgcgaag gtctttggaa ttcatgatgt
    10441 aggtgccgtg ttcgtcttcc ataatcggca tcatttcgcc cgggcggttg gattcttcaa
    10501 tcaggaaaac tttgtcggcg tagggatggc gtttttgacc gttgatgcct tcaaagtttt
    10561 ggttggcttc ttcttgggct ttttcaaagt tgaaaccttg cagaagctgg gcatcgcctg
    10621 catcgctttc cgtggcattg tgaaccttgt aatcccaacg gcaggagttg gtgcaggtgc
    10681 cttggttggg gtcgcggtgg ttgaaatagc ccgacaatag gcaacggcct gaataagcga
    10741 tgcacaatgc gccgtggatg aagacttcga gttcgatgtc ggggcattct tggcggattt
    10801 cggcgatttc ttccatactc aattcgcgcg acagaataat gcgttcgacg ccgatgtttt
    10861 gccagaattt cacgccccaa tagttggtgg tgttcgcctg tacggacaga tggatcggca
    10921 tttccggcca tttttcgcgc acggtcataa tcaaacccgg atccgccata atcagcgcgt
    10981 cgggtttcat ggcaatcagc ggctccatgt cggcaacgaa ggttttgagt ttggaattgt
    11041 gcggcagggt gttgacggtt aaaaagaatt ttttgttgcg ctcgtgcgct tctttaatgc
    11101 cttgttctaa aacatcaagt ttggcaaatt cgttgttgcg ggcgcgcagt gagtaacgcg
    11161 ggctgccggc gtaaacggcg tctgcgccgt agtcgtaggc ggcgcgcatt ctttccaatc
    11221 cgccggcggg caataagagt tcgggtgctt tcatcgtgtt tccttttcgg ttgaaacccc
    11281 gccctttagg gcagtagaat cagactttat ttgggagggg cgtaacccct tccgaatcag
    11341 ggcaacatat agggcgacgc tttatgtgtc gtcctgtgtg ttgaaacatg atttgtatgc
    11401 tttgtaaaaa tgtttcagac ggcattgctg caaatgccgt ctgaaaaggg tttgaggatg
    11461 ggcggattat ccgccttttt gtattttcgg tcaattttga cgggaaattt ataaggattt
    11521 atcaagtatt tgcctgcttg ggatataatg cggtagtttg aatgcgagag agaatgtgat
    11581 gaatcctaaa atcgtgtttt tcgacattga cgatacgctg taccgaaaat atacggatac
    11641 tttgcgccct tccgtgaaaa cggcggtggc ggctttgcgc ggcaaaggta tattgacggc
    11701 gttggcaacg gggcggtctt tggcgacgat tcccgaaaag gtcagggata tgatggcgga
    11761 agcgggaatg gatgccgtgg taacgataaa cggacagttt gcgctgctgc acggaaaaac
    11821 cgtgcgcgaa gtaccgatgg atgccggttt gatgggcagg gtttgcgcgc atttggatgg
    11881 cttgggcatg gattatgcgt ttgtcggcgg agaggggatc gctgtgtccg cgctgtcgga
    11941 atgcgtgtgc cgcgccttga agcatatcgc cagcgatttt tttgccgata aggattattt
    12001 ttcaagcaaa ccggtgtatc agatgctggt gtttgcggag gaaaacgaaa tgccgctttg
    12061 gtcggatatt gtggaacggg aaggcttgaa aacggtgcgc tggcacgagg aagcggtcga
    12121 tctgctgcct gcgggtgcgt cgaaaacgga cggcatcaga agcgtggttg aagcattggg
    12181 atgggaaatg gcagacgtga tggcgttcgg cgacggtttg aacgatgtgg aaatgctgtc
    12241 agaagtcggg ttcggcgtgg caatgggcaa cggggaacag gcggcgaaag aagcggcgaa
    12301 atatgtttgc cccagcgttg atgaagacgg cgtgttgagg ggcttgcaag atttgggcgt
    12361 gatttgaacg catcataaca accctgccgg tttcagacgg cagggtcggt tttcagccct
    12421 tcatacagcc ttcgttttga agcagggtaa ataagggcgc gccgcttgca cggatattct
    12481 tgccgccttg aaggtcggta aattccaaaa tggcggcggc ttcgacaatt tctccgccga
    12541 gtttgcggat cagttccagt ccggcaagca tcgtgccgcc cgtggcaacc aaatcatcga
    12601 ccagcagcac gcgcgaaccg agtttgacgg catcggtgtg gatttccacc gcagcttccc
    12661 cgtattcgag cgcgtagctt tgcgatacgg tttcaaaagg cagcttgcct tttttgcgga
    12721 tggggacgaa accgacgttg agctggtagg cgagtgccgc gccgataatg aagccgcgcg
    12781 cgtccaaacc ggcaacgatg tcgattttct gatccatata gcggtaaacc aataaatcaa
    12841 ccaaaagtcg gaagtattcc gcgctttgca ggacgggcgt gatgtcgtgg aataagatgc
    12901 ctttttgcgg ccagttttcg attttgcgga ttttgtcggc aagcgcgccg acactcatag
    12961 cttcgggatg aaccagcatt gcgtgttcca agtttgatgt ttttaacgcc atattgtagc
    13021 cgtcagcctg ccgcttgcca aatcaatcaa aacaatagcg gcaaaatatg ggcatcggca
    13081 aataggaaac cgagtgtccg ttgtgataca atcggctgga aaatatacag gtaaaaaata
    13141 tgtccgctta tcgaaaaggc aacatcttta tcatttcggc cgcttccggc acgggcaaaa
    13201 ccacgctggt gtcgcggctg ttggcaaacc ataacggttt gcgcgtttcc gtatcgcaca
    13261 cgacgcgtcc gccgcgtgaa ggcgaagtaa acggcgtaca ttatcatttt gtttctaaag
    13321 aagagtttga gtcgcttatc gcgcaggaag cttttttgga atacgcagac gtgttcggca
    13381 actattacgg cacaagcgcg gagggcgtga atgctttggc ggcggcaggc tatgacgtaa
    13441 ttttggaaat cgacgttcag ggcgcggcgc aggttcgcaa cgcgctgccg gaagccgtcg
    13501 gcatctttat cctgccgccg tctttcgatg tgcttgctgc acgtctgaac ggacgcggga
    13561 cggacagtcg ggaagttatc caaaggaggc tgtcgaaggc aaggcatgaa atcgaacagt
    13621 ccgtattgtt cgattttgtc gtggtcaatg atgacttggc gcgggcggag gaggatttgc
    13681 gccatattgt gaatgcctgc cgtctgaaaa ggtcgcggca actgggattt attgcagatt
    13741 tgttggaaaa ttcctaaaaa accgcgaaaa tacccagttt cctattttaa atatttttga
    13801 aagaaagcaa ataatatggc acgtattacc accgaagact gtaccggaaa aatttccaac
    13861 cattttgacc tgacattggt agcggcccgt cgggcccgcc agcttgaaaa tggcaatacg
    13921 ccgcttgtgg acgatgtccg caataacaaa ccgaccgtta ccgccttaag ggaaatcgcc
    13981 gccgggcata tcggtacgga actgttgacg cgcaataaat aaattctgcc ggaaacgcac
    14041 gtcggcacat tctgccgccg tgcagtccga cggtttgaaa cgaaaaataa aatatgttcc
    14101 gaaagccgtc tgaaatgcct gtccctcaac ccaccgcacc ttacgacccc ctgaccgccg
    14161 aagcgcgtgc cctgcttttc cataccgcct cctacctcaa gcccgaggaa caggcggagc
    14221 ttgaaaaagc tgtcgcctat gcgtttcgcg cccacgacgg gcaaacccgc aaaagcgggg
    14281 agccctacat cacgcatccg attgccgttg cgacgcagct cgccctttgg catatggaca
    14341 tacagggtct ttgtgcaggc gtgatgcacg acgtattgga agatacgggc gtgacaaaag
    14401 gggaaatggc ggcggtgttc ggcaatacgg ttgccgagat ggtggacggg ctgtccaaac
    14461 tcgaaaaact caaatttgaa gatcatgcag agcatcaggc ggagagtttc cgcaaactga
    14521 ttttggcaat gaccaaagat gtacgcgtca tcgtcgtcaa acttgccgac cgtctgcata
    14581 atatgcggac actcggttcg atgcgcccgg acaaacgccg ccggattgca agggaaaccc
    14641 ttgaaatcta tgcacagatt gccaaccgca taggtttgaa taacgcatat caagagcttc
    14701 aggatttatc gttccaaaac ctgcatccca accgctacga gactttaaaa aaagcgatgg
    14761 acaagagccg gaagaaccgg caggacgttg tcggcaaagt cttgcgcgca ttcggccagc
    14821 ggctggtagg cgcgaatata gaggccaaaa tcaaaggcag ggaaaaaaac ctgtacggca
    14881 tccatcagaa aatgatggcg aaaaagctgc gctttgccga ggttatggat atttacggtt
    14941 tccgcgtcat tgtcaacagc attccagcct gttatgccgc actcggcgca ttgcacaccc
    15001 tctatcagcc caagcccggg cggttcaaag actatatcgc cattccgaaa agcaacgggt
    15061 atcaaagtct gcatacgact ttggtcggcc cttacggctt gccgattgaa gttcagatac
    15121 gtaccaagga aatggatgct gttgccgaag gtggaatcgc cggacattgg agctataaat
    15181 catattctaa gacggtcgat caggcggtgc ttcacacaaa ccggtggctg aaaaatatct
    15241 tagatttgca ggcaagcagt gccaatgcca ttgagtttct cgaacacgtc aaagtcgatt
    15301 tgtttccgaa cgaaatctac atccttacgc caaaaggaaa aatcctaact ttgcccaaag
    15361 gggcaacgcc tgtcgatttt gcttatgcgg tgcataccga tatcgggcac aaaaccgttg
    15421 ccgcacgtat caacaatatc atgatgccgt tgcgtacgaa gctcaaaacc ggtgattctg
    15481 ttgaaattat cacatccgaa cacgccaaac ccaatcccgc gtggttgaat ttcgccgtgt
    15541 caggcagggc gcgcagcgcc atacgccaat atattaaaaa ccttaaccgg cacgatgcgg
    15601 tcgttttggg agagagcctc ttacaaaaag ccctgtccag tttgctgccc aaagatgtcc
    15661 tgctttcaga cggcatcaag gaaaaatatc ttgccgatct caacgacaag cagacatcgt
    15721 ttgaagaagt gctgtacaac gtagggatgg ggcataccct gcctgtttat gtcgccatgc
    15781 acattgccga gttggcaggg gagcatttcg gcagcgaggt caggctcagt tccattaaag
    15841 tcgatgggca ggaaagcggg catattcatt ttgcagagtg ctgccaccct gttcccggcg
    15901 attccatccg tttgctgttg gttaagggaa aaggcatgat tatccatagg gatacctgcc
    15961 cgacgttgtt gaagtccgat cccgaacagc agctggatgc agactgggaa aatatgaacg
    16021 ggcagaacta ccgtgtcggg cttcaagtcc aatcggaaga cagccacggc ctgttggcat
    16081 taatggcgca agcgatttcc gattccggtg cagacattga gtcggtcgaa acaccgtcta
    16141 aatcccagtc gggaacggaa ggttttgtcg aattcaaatt cttattgaaa gtcaagaatc
    16201 tgaatcaatt gaatcagatt attcaaaatc tgcatagtat tccatatatc cgcaaagtca
    16261 tcagaagttg aagcaggtaa aacgggttgc cttttgatta acaggcaacc cgttttatgg
    16321 tttatccgtt caattgctta tttgctcaaa tatacggcaa gcctttcaac aatccgcatt
    16381 gccgcttcat ctttacttgt ttcgggaaaa gacagttccc cgtcgtcccc gataatggta
    16441 atccggttgg tcggtttgcc cattgcgatt gaaacatcat tggcaacgat catcggtagc
    16501 tttttcttaa tacgtttttc ccgcgcatat gtcattacat tctccgtttc agcggcaaaa
    16561 ccgatgcaga acggcgggtt cggtaatgag gcaatagaag ccaaaatatc ggggttctca
    16621 tccaattcga tggataacgg tttggcattt ttatcttttt tgaatttttg agtactccta
    16681 ttcttaaccc tatagtctga gacggcggca acagaaataa aagcatcttg tttgtcgatt
    16741 aaacgatgca ctgcgcgatg catattttcg gcactgacgg cttgaaccgt atcggatatg
    16801 ccgaaaggca gcgcggtttg aagctgtccg tgaatcaggc tgacttctgc accggcggca
    16861 cggcacgccc gcgccaaagc cacgcccatt ttcccgctgg agatatttgt gatgcctcgg
    16921 acagggtcaa tggcttcaaa tgtcgcacct gcggtaatca agactttttt gccctttaaa
    16981 attttcggtg tccataaatc cggaagcaga tccagcaatt cggcaggttc cggcatcctt
    17041 cccataccat tttctccgca agcctgttcg cccaagcccg gcatatagac agtaatgccg
    17101 tctgaaacca gttgtgcgat attccgttgg ttggcagggt tgagccacat ttccacattc
    17161 atcgcgggcg cgatggcaag cggacatttc cgtgcggctg ccagactggt cagtaggtta
    17221 tctgccacgc cgttacagat ttttgccacg gtattcatac ttgccggcgc aatcagaaaa
    17281 acatccgcat tccgggtcag gttgatatgt tccataccgt ttgaaccgtt gccgccgtgc
    17341 gtgtcggtca ggacaggatt gccgcttaaa gcctgaaaag tcagcggaga aacaaattca
    17401 gttgccgagc ggctcataac caccgtaacc gaatgcccct gttttttcag cagtcgcacc
    17461 aactcgcaag acttatacgc cgcaatactg cccgttacac ctaaaagaat atgtttgccc
    17521 attttgacaa tttctaatta cgaagaaaaa acagcagcca ttctataaca aagtacggat
    17581 acattagata ccgtttggat acaa
//
LOCUS       DAWHRS010000025        17346 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWHRS010000025 DAWHRS010000000
VERSION     DAWHRS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17346
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.025"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..223)
                     /locus_tag="WIB82_000589"
     CDS             complement(<1..223)
                     /locus_tag="WIB82_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEZ3841927.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTED"
     gene            362..1312
                     /gene="trxB"
                     /locus_tag="WIB82_000590"
     CDS             362..1312
                     /gene="trxB"
                     /locus_tag="WIB82_000590"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ3841928.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            1468..1836
                     /gene="rpsF"
                     /locus_tag="WIB82_000591"
     CDS             1468..1836
                     /gene="rpsF"
                     /locus_tag="WIB82_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ3841929.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            1837..2139
                     /gene="priB"
                     /locus_tag="WIB82_000592"
     CDS             1837..2139
                     /gene="priB"
                     /locus_tag="WIB82_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ3841930.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            2146..2376
                     /locus_tag="WIB82_000593"
     CDS             2146..2376
                     /locus_tag="WIB82_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ3841931.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            2393..2845
                     /locus_tag="WIB82_000594"
     CDS             2393..2845
                     /locus_tag="WIB82_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ3841932.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(3038..3844)
                     /locus_tag="WIB82_000595"
     CDS             complement(3038..3844)
                     /locus_tag="WIB82_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ3841933.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAIMVFTAFSSMLGQHKKRAVDWKTVFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTVVAFKTLHTGPQTASRPLPKLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(3845..4591)
                     /gene="pssA"
                     /locus_tag="WIB82_000596"
     CDS             complement(3845..4591)
                     /gene="pssA"
                     /locus_tag="WIB82_000596"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ3841934.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(4646..5068)
                     /locus_tag="WIB82_000597"
                     /pseudo
     CDS             complement(4646..5068)
                     /locus_tag="WIB82_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5174..5656
                     /locus_tag="WIB82_000598"
     CDS             5174..5656
                     /locus_tag="WIB82_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ3841935.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(5708..6919)
                     /locus_tag="WIB82_000599"
     CDS             complement(5708..6919)
                     /locus_tag="WIB82_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ3841936.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            7142..9580
                     /locus_tag="WIB82_000600"
     CDS             7142..9580
                     /locus_tag="WIB82_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ3841937.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSSTD
                     LNGSRKILAHKDHL"
     gene            9788..11098
                     /locus_tag="WIB82_000601"
     CDS             9788..11098
                     /locus_tag="WIB82_000601"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ3841938.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            11194..11808
                     /gene="clpP"
                     /locus_tag="WIB82_000602"
     CDS             11194..11808
                     /gene="clpP"
                     /locus_tag="WIB82_000602"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ3841939.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCDRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLRL"
     gene            11832..12026
                     /locus_tag="WIB82_000603"
     CDS             11832..12026
                     /locus_tag="WIB82_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841940.1"
                     /translation="MPSETFRRHLFQAAFDMVSIPSSTGRKKDLEFFKGSPFPPVMDG
                     CRLKAKWGSSFCKGATLASS"
     gene            complement(12251..13516)
                     /gene="ispG"
                     /locus_tag="WIB82_000604"
                     /gene_synonym="gcpE"
     CDS             complement(12251..13516)
                     /gene="ispG"
                     /locus_tag="WIB82_000604"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ3841941.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSSLESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGLKRHQGKVIPIHSL"
     gene            complement(13532..14293)
                     /gene="pilW"
                     /locus_tag="WIB82_000605"
     CDS             complement(13532..14293)
                     /gene="pilW"
                     /locus_tag="WIB82_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ3841942.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(14296..15390)
                     /gene="rlmN"
                     /locus_tag="WIB82_000606"
     CDS             complement(14296..15390)
                     /gene="rlmN"
                     /locus_tag="WIB82_000606"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ3841943.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(15533..15958)
                     /gene="ndk"
                     /locus_tag="WIB82_000607"
     CDS             complement(15533..15958)
                     /gene="ndk"
                     /locus_tag="WIB82_000607"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ3841944.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(16017..17168)
                     /locus_tag="WIB82_000608"
     CDS             complement(16017..17168)
                     /locus_tag="WIB82_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ3841945.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLTEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         4312 a         4276 c         4633 g         4125 t
ORIGIN      
        1 tatcttccgt tttttccttc gcgccgtaac cggttttctc aatgattttg gcaatgtcgg
       61 ctactgaggt tttgctgtcg tcaaacacta cctgcgcctc ttcgctggcg aagtttaccc
      121 ccgccgattc gacaaaatct tttttgttca acactttttc aatgcgcgaa gcgcaggcct
      181 ggcaggtcat gccttcgatt tggaaacgga ttttttgttg cataagcgtg attcctgctt
      241 tgatattcag acggcagtta aggctgcggg atgccgtctg agcgccggta tggtcgaaat
      301 ttctcaaacg cgccgataat tcgagtagaa tccgaaaact tgacacagca aaggaaaaac
      361 aatgtcccaa caccgcaaac tgattatttt gggttccggc cccgccggat acaccgccgc
      421 cgtctatgcc gcgcgcgcca atttaaaccc cgtcattatt acaggtatcg cgcaaggcgg
      481 gcaactgatg accaccaccg aagtggacaa ctggcctgcc gatgccgacg gcgtgcaagg
      541 gccggaattg atggcgcggt ttctcgccca cgccgaacgt ttcggaacgg aaatcatttt
      601 tgaccaaatc aacgccgtcg atctgcaaaa acgcccgttt acactcaaag gcgatatggg
      661 cgagtacact tgcgatgccc tgattgtcgc aacaggcgcg tccgccaaat acctcggttt
      721 gccgagtgag gaagcgtttg caggaaaagg cgtttccgct tgtgccacct gcgacggttt
      781 cttctataaa aaccaagatg ttgccgtagt tggcggcggc aatacggcag ttgaagaggc
      841 actctacctt gccaatatcg ccaaaaccgt taccctgatc caccgccgca gcgagttccg
      901 tgccgaaaaa atcatgatcg acaaactgat gaaacgcgtg gaagagggca aaatcatcct
      961 caagctggaa agcaacctgc aagaagtact gggcgacgat cggggcgtaa acggcgcatt
     1021 attaaaaaac aacgacggtt ctgagcaaca aattgccgtc agcggcattt ttatcgccat
     1081 cgggcacaag ccgaataccg atattttcaa aggtcagttg gaaatggacg aagccggtta
     1141 cctgaaaacc aaaggcggca cggcggacaa tgtcggcgca accaatatcg aaggcgtatg
     1201 ggcggcgggc gacgtaaaag accataccta ccgtcaggcg attaccagcg cggcttccgg
     1261 ctgtcaggcg gctttggacg cggaacgctg gctgggcagc caaaacattt gaatgccgtc
     1321 tgaaaccgaa attttcggcg gaatgcaaat ttttcgcacc aaatccttga ttccgtcggc
     1381 aaaccccaat ataatgccgc ttctttaaac cttgccttct gctttcgcat ggcagaaggc
     1441 tgtttttacc atccataagg agataacatg cgtcattacg agatcgtgtt tatcgttcat
     1501 cccgatcaaa gcgagcaagt acccgctatg gttgaacgtt acaaaaccat gattgccgaa
     1561 gcaaacggta agattcaccg tctggaagac tggggtcgcc gccagctggc ttacccgatt
     1621 aacaaaatcc ataaagccca ttacgttttg atgaacatcg aaaccactcc cgaagtggtt
     1681 gaagagctgg aaaccgcatt ccgcttcaat gatgcagtat tgcgtcatct gaccatcaaa
     1741 accaaacacg ccgttaccga agcatcccct atgttgggtg gtgaaaaggc taagaacctg
     1801 ttgagcggtg cgtctgaaga agcggtcgcc caataattgg gattcaataa tcttgtttcg
     1861 cttgccgcgt taattgaaaa ggttttccct attcgatata cgcctgccgg aatccctgtt
     1921 ttagatatta ttttaaagca cgaatcgtgg caggaggaaa acgggcagca atgccttgtc
     1981 caattggaaa ttccggcacg gattttaggc aggcaggcgg aagagtggca gtatcggcaa
     2041 ggtgtatatg ttcacgtcga aggtttttta gctcaaaaaa gcagacgttc ccttatgccg
     2101 atgctcagga tacaaaatat tcaagaatat aaaggttaaa cgacaatggc tcgtcaatca
     2161 ttcaaacgta gaaaattctg ccgtttcacg gctgaaaaaa tccaagaagt cgattacaaa
     2221 caagttgatt tgctgaaaga cttcatctcc gaaaacggta aaatcattcc tgcacgcatc
     2281 acaggaacga aggcattcta ccaacgccaa ttggctgttg ccgtaaaacg cgcacgcttc
     2341 ctggctctgc tgccttacac cgaccaacac aaataatttt ggagattgaa tcatgcaaat
     2401 tattctgtta gaaaaaatcg gcggtctggg caacttgggc gacatcgtaa ccgttaaaaa
     2461 cggctacgcc cgcaactttc taattcccgc aggtaaggca aaacgtgcga ccgaagcgaa
     2521 tatgaaagag tttgaagcac gccgcgcaga actggaagcc aaacaggctg aaattttggc
     2581 agatgcccga gtccgtcagg aaaaattgga cggtcaaacc gttaccgttg ctcaaaaagc
     2641 tggtgtggac ggtcgcctgt tcggttccgt taccaatgcc gacattgctg ctgcaatcgt
     2701 tgctgccggc atcgaagccg tgaaagcaaa tgtacgtctg ccgaacggtc ctctgaaagc
     2761 cgttggcgag tacgaagtgg aagtggcttt gcacactgat gccgttgcta aaattaccgt
     2821 tgccgtcgtt gccgcaaccg agtaattgaa attatcagat gccgtctgaa accctgccga
     2881 ttgtcggaat gtggtttcag acggtatttg ttttgttttt cgtatattct gcaaaggtct
     2941 cggtcaggtt gtctgtcctt ttgtcggaac aggagactgg gcagctttgt cctgattaat
     3001 tgcttgtgca aacggtaaaa aggttttttc gtgtgtttta aagcaggttg tacagcattt
     3061 ttccggcaat caaaagcaac ataatgccga aggatttttt gagtttggca gaagaaagtt
     3121 tgtgggcggt tttgacaccg agcggggcaa aggcaatggt tgccgcgctg aggacggcga
     3181 cggcgggcag gtaaaggaag cccagcgacc cttcgggcaa tcctgcaata ttcaggccgt
     3241 tgagcagata cgatattgcg ccggagagtg caatcggcca ggcaaggccg gatgatgtgc
     3301 cgatggcttt atgggcgggg aagccgcagt ggattaagaa ggggacggaa agtgaaccgc
     3361 cgcctatgcc gacccagctc gacattgcgc cgaacagtgt ggaaaccgca gtcagtccgg
     3421 gcagcttggg cagcgggcgg gatgccgtct gagggccggt atgcagtgtt ttgaatgcga
     3481 cgacagttaa aaacaggatg aagaagattt gaagcccgaa tgcggggata tattttgcgg
     3541 agagtgcgcc cgcgaatacg ccgaatatca tacccggcat catcgcaaat acggttttcc
     3601 agtcgaccgc tcgttttttg tgctgcccca acatactgga aaaggcggtg aagaccatga
     3661 tggcgaagga tgtgccgacg gcgaggtgtt gcgcgtaagg atgttgtgcc aaaccctgca
     3721 aatcaagcac ccataaaacg acagggacaa tcagcgtgcc gccgcctacg ccgaacaggc
     3781 cggcgataaa acctgccgca ctgcctacgg caagcaggat taagataatg tcccaatgcc
     3841 acatttaatc cgcctttctg tattttttcc agaatcggcg tgccgccata atgtagccgg
     3901 acaggctgta tccgagaaag aacaggaaga ggacgagcga cggttcccaa gtgaccagaa
     3961 gcagcagtaa gacggcaagc agcattccga caaaggggac ttgtctgcgg atgttgattt
     4021 ctttaaaact ccaaaaaggg atttggacaa tcattgacag gccggcaaac agtgtgatgc
     4081 ccaatgccca ccagtggacg gcggggaatt tttcgacgct gtggttgacc caaatcagcc
     4141 cgacaatcag cgcggcggca gtcggactgg gcacgccgat aaaccagcgt ttgtccacct
     4201 tgccgatgag cgtgttgaac agggcgaggc gcagggctgc gcaggcgcag tagatgaagg
     4261 cgacggaata accgattttg ccgaactgcc aaagctgcca tttgtaggca ataagcgcgg
     4321 gggcaacgcc gaagctgacc atgtcggcaa ggctgtcgag ctgctccccg aacgcgcttt
     4381 ggctgttggt cagccgcgcc acgcgcccgt ccataccgtc caaaagcatg gaaatgaata
     4441 cggcaatagc ggcggtttca taacgtccgt gcatggattg ggtgattgcg taaaacgcgg
     4501 aaaacagcgc ggcgatagta aaggaattgg gcagcaggta gatactgttt tgacgcaggg
     4561 agcgttgcgg ggtgttggtc ggggtttcca tagctgaagt gtctcacgga cgtggagaat
     4621 cgatagggga ttataaccca aaccattatc gaacaatacg ccctttagga tgaagccctc
     4681 ggaggcggtt tgtttttctg atggcggatg ctttgcgggg cggtttgact ctgcctgccg
     4741 ccgggtgtgt tgtttcggat ttttgccgga tggatgttgt tgccgggcat ttctgggctt
     4801 gccggaaggt atcgggggga gggcatcgga atatgcggcg cgccgttcaa actggggcga
     4861 cgtttcccta ttttcagggg gttgcaggtt tcaacaaatt gaaagatatt ttttgcattt
     4921 ccgaccgtat gctgattttg aaacgggata aattatagga caaggttttt cgcccgccgc
     4981 aaaggttggc cgcaatgcag tctgaaaaca acggcttgcc gctaaggcgg cggtatgcgg
     5041 atttgcgctg ccgaaaccgg tttgccatac ccggcggtgc aatctgcggg gagggataag
     5101 ccgataaggc gggacttatc ctgttttttg tttataatgc aacagaattt ggacgaaccg
     5161 tcgggaattt atcatgatga aaaagatact ggccgtatcg gcactatgcc tgatgactgc
     5221 ggcggcacgg gctgccgata cttacggcta tctcgccgtt tggcagaatc cgcagaatgc
     5281 ggacgatgtt ttgcaggtta aaactacaaa agaagattcg acgaaaagcg aagcgtttgc
     5341 cgagttggaa gccttttgca aaggtcagga cacgcttgcg ggcattgccg aagacgagcc
     5401 gaccggatgc cggtcggtcg tgtcgctgaa caatacctgt gtcgcgctgg catacccgaa
     5461 agccttgggc gtgatgcgcg ttgaaaacgc cgtcgtgatt acttctccgc gttttacgag
     5521 cgttcatcag gtcgcactca accagtgcat caaaaaatac ggcgtacagg gacaatgcgg
     5581 cttggaaaca gtgtattgca catcttcttc ttattacggc ggaactgtgc gctctttgat
     5641 tcaaaatctc aaataaaacg gaaaatgccg tctgaaagat gttcagacgg catttctata
     5701 tcgacggtca ggattctttc ggatcgggca gcaggctgtt caacataatg gcaagtacgg
     5761 cgcacaagcc cacgccggca aagctgaagc tgcccaattt gagcgtcatg ccgccgatgc
     5821 ccgtggtcag taccgagctg acgatgacca ggtttttcgg cagcatcaaa tcgactttgg
     5881 catcaatcag cgttttcacg cccaaagaag caatcgtgcc gaacagcagc agcataatgc
     5941 cgcccattac tggcatcgga atggaagcca aaaacgcatt gaatttgccg aaaaacgcca
     6001 tgcagacggc aaaaaccgcc gcccaagtca tgatgacggg gttgctgttt ttggtaatca
     6061 tcaccgcacc cgttacttcg ccgtaggtcg taaccggcgg gccgccgatc agacccgcaa
     6121 cgcatacgcc caaaccgtcg cctgcaaggg ttttgtccaa gcccgggtct ttcgtatagt
     6181 ctttccccgt cacattgccg attgccatga tgccgccgat gtgttcgatg gcgggggcga
     6241 cggcaacggg cagcataaac agtgcagcct gccagttgat ctgaggcgtt tcaaaatggg
     6301 gaacggcgaa ccagggcgcg tgtgcaatgc ttgccgtgtc caccagtccc atcagcagtg
     6361 ccaaaacata acccgaagcg acaccgatca agatgggaat cagcttcatc atcctgctgc
     6421 cgaaaaccga tacgatggcg gtaacggcaa aggtaaagcc ggaaagaatc agcgaatcgg
     6481 tatagtcgat gacctgtttg ccgtccgcct gacccattgc catgctgctt gccgccacgg
     6541 caacagacag accgatgacc atgatgacgg ggccgatgac gaccggaggc agcagtttgt
     6601 gtaccgctgc cagtccgcgc caacggatca gcgcggcaaa cacaaaatac ataaagccgg
     6661 cggcaaacag tccgaacatg gtggaaggca gcccccattc gccgacggag tagataatcg
     6721 gtgcgataaa ggcaaacgaa gaaccaagaa aaatcggcac tttgcgtttg gttgtgattt
     6781 ggaacagcag cgttcccaag cctgcgccca aaagcgcaag agccggattc agaccggtca
     6841 gcaggggaac cagcaccatt gcgccgaatg ccacaaataa aatctgtgca ccggaaacgg
     6901 caagtttcag ttggttcata ccatcttctt tcaatcggtt caaacggcac ggattatact
     6961 ggtttgccga gggtctgcgt aaaaatacat acgtcgggca acagatatgc cgtctgaaag
     7021 gcaatgtctg tctgttgcag gatgaaagca ggagaaatgg agtataatcc gacactttcc
     7081 atattcaatc cgcaagccgt ccggcttcct tatttcagac ggcattttcc ggtaatacgg
     7141 catgacagaa aaatcccata aaaaaacagc aaaaggcagg gcgggaagcc cgtccccaac
     7201 gtccgcacgc aacaagaaag ccgacaacgg cgcacggggc aataaagttt ccgagcggct
     7261 caaggcggtc aaagagttgc agaaaaccga aaccaaaaag gcgcgtcccg aacatgtcgt
     7321 caaccttatc ggcgacgcac tgtggctgat gggtttggcg gcaaccctgt atttggcgat
     7381 ttccctgatc agtttcgata tgggcgatcc gtcttggtcg cacagttcgc cggttgtgga
     7441 agatgtcgcc aattggggcg gactgttcgg cgcgtatgtt gccgatgtcg gctattatct
     7501 tttcggctgg tcgttctggt ggtggatagc ggctgcctgc gtcgtgctgt ataaaaattt
     7561 ccgcctgcac gcaaaacaga cggaaaacga ggcatacaac cacaaaatcg ctgccgccgc
     7621 gctgtttgtc ctgacggtct tcagccccgt cttggagtat tttgtgctgg gcggaaaata
     7681 tgccgactcc ctgcctgtcg gagcaggcgg tatggtcggc atacgcgtcg gcgcagtgtt
     7741 tgcgtggctg ctggggaaat cgggcagcct gctgattatc ttggttgttc tgctgttgtc
     7801 gttgtccctg ctggtgcaga tttcatggct ggaatttttg aacggtgcgg gcagggcggt
     7861 tcaaaaccgc ctgagtgcct tatccggcaa ggtcatggct ttaggaaaac gccggccgaa
     7921 taccaaaaca gacggtgtcg atacccaaaa tacacggcgc atggtaaaag aagccaagaa
     7981 tattacggcc aaacccgttg ccttgcccga aggcagcagc agcaaccgca aatccgtcgc
     8041 ggtttccgtc gcgccgccgc ccaaaattca ggtttctctg tttgaagatg acgaacctcg
     8101 gcaggcgggc gaataccaca agcctacatt gaacctattg cggattcctg acagcgaacc
     8161 cgtcagcatc aatcccgccg aattggagcg cactgccgaa ctgatcgaat ccaaactggc
     8221 agaattcggc atcggcgtac aagtcgtatc cgccacatcc ggccccgtca tcacgcgcta
     8281 cgaaatcgaa cccgcgcaag gtgttaaagg cagccaaatt gttgccttgt cgaaagattt
     8341 ggcacgctct atgtcgctgc agtccgtgcg tatcgtcgaa accatcgcag gtaaaaacac
     8401 gatgggcatc gagttgccca acgacaaacg ccaagacgtg atgttgagtg aaatcttgtc
     8461 ctcgcccgtg tttgccgaag ccaaatccaa gctgaccgtc gcgctgggca aagacattgc
     8521 cggtaccccc gttgtcggcg acttggcgaa aatgccgcac cttttggtcg ccggtatgac
     8581 tggttcgggc aagtccgtcg gcgtgaacgg catgattatg tctatgcttt tcaaagctac
     8641 gcccgacgaa gtccgcttca ttatgataga cccgaaaatg ctcgagttga gcatttacga
     8701 cggtattccg cacctgctct gtcccgtcgt gaccgatatg cgcgaagcag ggcaggcgtt
     8761 gaactggtgc gtcgccgaaa tggaaaaacg ctaccgcctg ctttcccatg ccggtgtgcg
     8821 taatttggag ggcttcaacc aaaaagtcga agccgcaaaa gcggcaggca agccgctgct
     8881 caatccgttc agcctgaacc ccgacgagcc cgagccgctg gaaaaactgc cgttgattgt
     8941 ggtcgttatc gacgaacttg ccgacctgat gatgaccgaa cgcaaagccg tcgagcagca
     9001 aatcgcccgt ctcgcccaaa aagcgcgcgc cgccggtatc catatgattg tcgccaccca
     9061 acgtcccagt gtcgatgtcg ttaccggcct gattaaagcc aacatcccga cgcgtatggc
     9121 gtttaccgtg caaagcaaaa tcgacagccg taccatcctc gaccaaatgg gcgcggacga
     9181 actgctcaaa tatggcgatt cgctgttcct ccagcccggc agtgccgaac cgactcgcct
     9241 gcaaggcgcg tttgtttcag acgacgaagt acatcaagtc gtcaactacg tcaaatcgca
     9301 agccccagcc gactatattg aaggtctgct cagcggcgag gccgcgctgg aaactgccaa
     9361 tatcgttaat ccgaatgcag acagcgacga attgttcgat caggcagtcg cctatgtttt
     9421 ggaaagcaaa aaaacctcca tttcgtcttt gcagcggcag ctgcgcatcg gctataaccg
     9481 cgcggcaaac ctgatggagg cactggaaaa tgcgggtgtc gtttcttcca ccgacctcaa
     9541 cggcagccgt aaaattttgg cgcacaagga ccatttgtag cccgtattgc aaaatgccgt
     9601 ctgaacggcg gaattggcgt ttcagacggc atattatgtt tcaggcgaaa cattgtgata
     9661 tacttgccag ctaaaatttc ccctttgcgg caatgcggtt caaatatcgt accgttgcgc
     9721 tgtttgcttc cccatgtagg gaagaaagtt tatcatttta tcaacacaac aaatttaagg
     9781 gcttatgatg agcgtaactg ttgaaacttt agaaaatctg gaacgcaaag tagtgttgtc
     9841 cctgccttgg tccgaaatca acgcagaaac cgataaaaaa ctgaaacaaa cccaacgccg
     9901 tgcaaaaatc gacggtttcc gtccgggtaa agcaccttta aaaatgattg cccaaatgta
     9961 cggtgcgagc gcacaaaacg acgtgatcaa cgagctggtg caacgccgct tctacgatgt
    10021 tgccgttgcc caagagttga aagtggcagg cttcccccgt tttgaaggcg ttgaagaaca
    10081 agacgataaa gagtctttca aagttgccgc catttttgaa gtgttccccg aagtcgttat
    10141 cggcgatttg tctgcacaag aagttgaaaa agtaaccgct tccgtcggtg atgccgaagt
    10201 ggaccaaacc gtagaaatcc tgcgcaaaca acgcacccgc ttcaaccatg tcgaacgcga
    10261 agcccgaaac ggcgaccgcg tcatcattga ctttgaaggc aaaatcgacg gcgaaccttt
    10321 tgccggcggc gcatccaaaa actacgcctt cgtattgggc gcaagtcaaa tgctgcctga
    10381 atttgaagcc ggcgtagtcg gcatgaaggc tggcgaaagt aaagacgtta ccgtcaattt
    10441 ccctgaagac taccacggta aagacgttgc cggtaaaact gccgtgttca ccattacgct
    10501 gaacaacgtt tccgaagcga ctctgcctga agtcgatgca gattttgcaa aagccttggg
    10561 tattgcggat ggcgacgttg ccaaaatgcg cgaagaagtg cagaaaaacg taagccgcga
    10621 agtggaacgc cgcgtaaacg aacaaaccaa agaatccgta atgaacgcgc tgctcaaagc
    10681 cgtagagctg aaagcacctg ttgctttggt caatgaagaa gccgcacgct tggcaaacga
    10741 aatgaaacaa aattttgtta accaaggtat ggctgatgct gccaacttgg atctgccttt
    10801 ggatatgttc aaagaacaag ccgaacgccg cgtatcttta ggtctgattt tagccaaact
    10861 ggttgacgaa aacaaactgg aaccgactga agagcaaatc aaagccgttg ttgccaactt
    10921 tgcagaaagc tacgaagatc ctcaagaagt gattgactgg tactacgcag atccttcccg
    10981 cctgcaagcc ccgacttctt tggcggtaga aagcaacgtc gttgatttcg ttttgggcaa
    11041 agccaaagta aatgaaaaag ctttgtcttt tgacgaagtg atgggcgcgc aagcctgatt
    11101 atcctgaaaa tgccgtctga aggcagtttt tgaaagcacc gaagccgctt ttaaagctcg
    11161 ctttggtgct tttccatcat gaaaggagac gaaatgtctt ttgataacta tcttgtccct
    11221 accgttatcg agcagagcgg tcgcggtgag cgtgcattcg atatctattc ccggcttttg
    11281 aaagagcgca tcgtattctt ggtcggaccg gtaaccgacg agtccgccaa tctggtggtt
    11341 gcccaactgt tgtttttgga aagtgagaat ccggataagg atattttctt ctatattaac
    11401 tcgccgggcg gttcggtaac ggccggtatg tcgatttacg acaccatgaa tttcatcaag
    11461 cccgatgtat cgactttgtg cttggggcag gcggcaagta tgggcgcgtt cttattgtcg
    11521 gcaggcgaga aaggcaaacg ttttgcccta cccaacagcc ggattatgat tcaccagcct
    11581 ttaatcagcg gcggtctggg cggtcaggca tccgacattg aaattcacgc acgcgaactt
    11641 ttaaaaatca aagaaaaact caaccgcctg atggcgaaac attgcgaccg cgatttggca
    11701 gatttggaac gcgacaccga ccgtgataat ttcatgtctg ccgaagaagc caaagaatat
    11761 ggtttgattg accagatttt ggaaaaccgc gcttctttgc ggctttaata aaagaaacct
    11821 gagagaaacc gatgccgtct gaaacgttca gacggcattt gtttcaagct gcgtttgata
    11881 tggtatcgat accgtcatcc acgggacgga agaaggattt ggagtttttt aaaggatccc
    11941 cgttcccgcc tgtgatggac ggatgccgtc tgaaagccaa atggggcagc agtttttgca
    12001 agggcgcgac actcgcatcc tcctgaatct gccggtacgg ttattgagaa aagacaaggg
    12061 caggaagcgg tatcccgcct tccttattta tacctgcaat tcatctccgt atttgccgat
    12121 tgtattttca tattgcgcct ccattcctta tcggtttaaa aaaatgcctt ttccaatggg
    12181 atgggtttta tatcaatgaa aataaacggt ttttatttaa aaatagccaa ggtaggctgg
    12241 cggttggatt ttatagggag tgtatcggga taaccttccc ttgatggcgt ttgagtccgt
    12301 tctccccata attggttttg acatactctt caacaatagc cagaaattcc gttgcaatgt
    12361 tgtcgccttt cagcgttact ttacgttcac catctacata aacaggggca acgggtgttt
    12421 ctcccgtacc gggcaggctg atgccgatgt cggccaattt gctttctccg ggcccattga
    12481 caacgcagcc cattacggca acgttcaggg attcaacccc aggataaagg gtacgccata
    12541 tagacatttt ttggcgcagg taattttgaa catcttgtgc cagctcttga aatacggtac
    12601 tggtggtacg cccgcagccg gggcaggcgg taaccatcgg cgtaaacgaa cgcaatccca
    12661 tagtctgtaa aatctcttgc ccgacgacga cctcctgagt acgcgggctg ccaggttccg
    12721 gagtcagtga aatgcggatg gtgtcgccga ttccttcttg aagcaagacg gataatgccg
    12781 ccgttgatgc gacaatgcct ttgctgccca taccggcttc ggtcaaaccc aaatgcagcg
    12841 gataggcgca acggctgccc agttcgcggt aaacctgaat caaatcctga accgcgctga
    12901 ctttgcacga caggatgatt ttgtcttcgg gcagtcccaa tagaacggct ttttcggcag
    12961 attccaaaga ggagacaatc agtgcttcct tcgtcacttc ttcgggcggt ttcggcgcgg
    13021 aagaagcgag gttggcatcc atcatacgtt tggcgaggct ttgatccaaa gaaccccagt
    13081 ttacgccgat gcggacggct ttatcgtttt cagcagcagt ccgaatcata aaggcaaatt
    13141 tttcatcgcc ttttacgcct ttgccgacat tgccgggatt gatgcggtat ttggacaatg
    13201 ctttgccgca ttcgggaaat tccgccaaca ggcgttcgcc gttgaagtgg aaatcgccga
    13261 taagcggtgt ggcatagccc atatcgtcca agcggcggcg gatttcggca actttggacg
    13321 cggcttcggg gctgttgacg gtaatacgca ccatttcgga tccggcatcg ctcaattcct
    13381 taatctgcaa tgcggtggct tttgcatcgg cagtgtcggt gttggtcata gattggataa
    13441 cgacgggtgc ttctgaaccg acggtaatat gatcgatgcg gacttgatgc gtcttgcggc
    13501 gttggagtgt gttcatatgg tttgaatctg tttattgacc ggtgaggacg gtttgcaatt
    13561 cttccgagta ggggaaattc gcctgcaatt gtgcttcata ttcgtatgcc gcctgtgcgt
    13621 tgccgagggc tttggcaatt ttccagccta gcagcaaatc atcggcctga aggacttcta
    13681 ccctgctttg gtatttttta aagtagtaat cggcatcgcc caactgcccg gccagcattt
    13741 tggtgcgcgc cagttcttta aatgcgggtg ggaactgcgg ctgggcggcg agggaacgtt
    13801 tcaaataggc ttccgccaat ccgaattgcc cctgttttgc gctgcatatg cctttattca
    13861 ggttggcaat ataaggggtc gggtaggtgg ggtcggccag ggctttgtcg aaatatgcca
    13921 tagattcggc agggcggttg agcctgccgc acaggaacca gccgtagttg ttgttgattt
    13981 cggcactgtc gggtttgatg gagagggctt gccggaaact ttcctgcgcc ttgtcgttaa
    14041 ctttcaggta ttgatagatt tcggcacgga ccagccaggc aagctcgttt ttagggtctg
    14101 atttcaaggc gtcttcaata cttgccgtcg cctgacggta gtcctgaccg cgcatatatt
    14161 ccattgccaa ctgggttttg atattggaaa cctgattggc tttttctgcc cgcgaggggc
    14221 ggtaggaagt gctgcacgcg cccaaggcaa ggacaagtaa taaagagatt cgtttggatg
    14281 gcttaaaagg cataattacc cctgttgtcc gattaaaatc tgctgccatt tttgttggcg
    14341 gcgcgtttta tcctgaacct gccccgccaa ctgtccgcag gcggcatcga tgtcgtcgcc
    14401 gcgcgttttt cgtacggtaa cgacaaatcc tgcctgctgc aaaatatcgc ggaacacacg
    14461 gatgttctca ttgctggagc gttcgtatcc ggagtttgga aagggattga acggaatcag
    14521 attgaacttg cagggaacat ctttgaccag ttcgatcagt tcgcgcgcat gttgcgcctt
    14581 atcgtttatt ccgtccaaca tgacgtattc gaaagtgatg aaatccctgg gtgctttgac
    14641 cagatagcgt tggcatgcag ccatcaattc tttcaaggga tattttttgt tcaacggtac
    14701 gatttggttg cggacttcgt cattggaagc gtgaagggaa accgccaaag ccaccggcat
    14761 gacatcgcgc aacctgtcca tttggggaac catacccgaa gtggaaacgg ttacacggcg
    14821 gcggctcaaa ccgtagccgt ggtcgtccag cataatgctt aaggcggtaa cgacattgtc
    14881 gaagttcgcc atcggctcgc ccatacccat catcactaca ttggaaatta cgcgttcgtt
    14941 tttcggtgta acacccatcg ctttgtttgc ccaccacaac tgtccgatga tttcggcagc
    15001 agtcaggttg cggttgaagc cctgccggcc ggttgagcaa aatgtacatt ccaaagcgca
    15061 gccgacttgc gaggaaatgc agagcgtgcc gcgatccgat tcggggatga agacggtttc
    15121 caccccgttc cccgtcccga catctagtag ccattttcga gtgccgtctg aagatttttg
    15181 agacatcatc agcttgggaa tttcgattcc tgcctgttcg ttcagtttat ggcgcaacga
    15241 ttttgccaaa tcggtcattt cgtcaaaatt ttgcgcgccg gattggtgca tccaacgcat
    15301 aacctgtttg gcacggaaag gtttttcgcc catatcggca aaatgtcggg tcagcccttg
    15361 aaggtcgtag ttgagcagat tggttttcat gtgtttgtgt ttttcttaaa ttaaggcttc
    15421 aaataaaaaa ttgcagggca ggcaagtttg atttgaagcc ttttgcgaca gagttttcag
    15481 acggcatgga tatatttatg ccgtctgaag aggagttgtg cggtgtattg tatcaacggg
    15541 ggcagatttc ggtttggctg aaaaagtagg caatttccaa agcggcattt tccacgctgt
    15601 cggaaccgtg tacggcatta atgctgaccg aagtggcaaa gtccgcgcgt atcgtgcctt
    15661 cggcggcttc ggaaggatta gttgcaccca tcagttcgcg gtttttcagg acggcgtttt
    15721 caccctctaa tacctgaatc ataaccggac cgccggtcat aaattcaacc aatccggcgt
    15781 agaaggggcg gtctttatga accgcataaa attcttgcgc ctctttgagg gtaagctgct
    15841 tcattttggc ggcaacgatt ttcagaccgt tctcctcaaa gcggctgtat attttgccga
    15901 taacgttttt gccgacggca tcgggtttga tgatggagat ggtacgttca atcgccatgc
    15961 tatatcctta tttttactta agaagaatca aatcggtatt ctatcaaaaa aataacttat
    16021 ccgcctgaag ccttgggaga tagggtcagg tgcggctgtt ccaaataaac ttcagactgc
    16081 atttcgacca tccggctggc ggtgcgggta aattcttcgg caaaatcgcc ttccgtatag
    16141 atatggttga catctaccgc gccggtggca cacagtttga cccggaaatc gtagagtacg
    16201 tcaatcagcc aagtcagccg ccgcgcctcc gccttttctt gcggtgagag ttgttccaaa
    16261 cctgaaataa aaaccatttc ataatgttcg gtcaaataca gatagtcgga ctgtgagcgg
    16321 gggccgaagc acagtgcgcg gaaatcaaac catatggcac ggccggactc ggctttgtgg
    16381 ggaatctccc gaccgtggat ggtgctgatg ccggggttca aatcggtaat gcctgtcatt
    16441 tctttgaaca gttttgccag ttttgcctca ttttcttcat tggcaggcgt aaagaaaatc
    16501 tcggcggggc ggagggtacg cagtcggtag tcttcaccgc cgtcaacgtt taagacggtc
    16561 aggctggact cgatgagcgc gattgtggga agaaaactgc tccggttttg accttgcggg
    16621 tagagttcgg aaggcgcgta gtttgaagtc gccaccaaaa caacgccctc gttaagcagg
    16681 ttttccagca gacggcctaa aatcattgca tccgcaatat cgctgacatg aaattcgtca
    16741 aaacataata cgcgggtttc tttggcaatc tcggcggcaa cggatttcaa cgggttgctt
    16801 tcgcttttca gggttttcag cctctggtgg atttctgcca taaaggcatg aaagtggacg
    16861 cggcgtttgc ggcggtaagg gaggcagccg aaaaaagcgt ccatcagaaa gcttttgccg
    16921 cgtccgaccc cgccatagaa ataaagccct ttggggactt gcggggaacg caaactcctg
    16981 cctaaaaaac ggtttctttt gcgtttgaac atcatcaatt cggtccaaag ccgatcgagg
    17041 tgttcgatgg cggctgcctg cgcgtcgtcg cggatgaagt tgggcagttg tgaggcagcc
    17101 tgataccaag tcagcgggct gtgattttca aacggcgggg ctttaaaaag ttggtctcta
    17161 ttactcatat ttaaccttgt tatacttctt gcggctaaga aagaaaatgc ttaccgtcgg
    17221 tattataaat gatttccttc aaacttggca ttttccgtgg aaaagaataa tgacgcgcac
    17281 aagggggtat aagcaaaggc ggcgtttcaa tatggtgcag atatgagctc aaatgccttt
    17341 tatagt
//
LOCUS       DAWHRS010000026        16940 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWHRS010000026 DAWHRS010000000
VERSION     DAWHRS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16940
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.026"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(52..717)
                     /locus_tag="WIB82_000609"
     CDS             complement(52..717)
                     /locus_tag="WIB82_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3841946.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYAEMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(917..1813)
                     /locus_tag="WIB82_000610"
     CDS             complement(917..1813)
                     /locus_tag="WIB82_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ3841947.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVARLTMTVYLPAEQKGTHMS
                     RFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEMKDGAYGYSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            1900..3057
                     /locus_tag="WIB82_000611"
     CDS             1900..3057
                     /locus_tag="WIB82_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ3841948.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFTLHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(3120..4121)
                     /gene="hemB"
                     /locus_tag="WIB82_000612"
     CDS             complement(3120..4121)
                     /gene="hemB"
                     /locus_tag="WIB82_000612"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ3841949.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(4304..4588)
                     /gene="yhbY"
                     /locus_tag="WIB82_000613"
     CDS             complement(4304..4588)
                     /gene="yhbY"
                     /locus_tag="WIB82_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ3841950.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            4698..5318
                     /locus_tag="WIB82_000614"
     CDS             4698..5318
                     /locus_tag="WIB82_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ3841951.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLAGPSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSLYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            5382..7349
                     /gene="ftsH"
                     /locus_tag="WIB82_000615"
     CDS             5382..7349
                     /gene="ftsH"
                     /locus_tag="WIB82_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ3841952.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADTAEDN
                     APHAPAREETEAPAPADTASTESEQQPENKA"
     gene            7570..8001
                     /locus_tag="WIB82_000616"
     CDS             7570..8001
                     /locus_tag="WIB82_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ3841953.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            7994..8272
                     /locus_tag="WIB82_000617"
     CDS             7994..8272
                     /locus_tag="WIB82_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ3841954.1"
                     /translation="MLEIEIVYGLSDKQVLKNMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            8325..8903
                     /gene="pth"
                     /locus_tag="WIB82_000618"
     CDS             8325..8903
                     /gene="pth"
                     /locus_tag="WIB82_000618"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ3841955.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            8964..9170
                     /locus_tag="WIB82_000619"
     CDS             8964..9170
                     /locus_tag="WIB82_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ3841956.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            9167..9529
                     /locus_tag="WIB82_000620"
     CDS             9167..9529
                     /locus_tag="WIB82_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ3841957.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            9760..11175
                     /locus_tag="WIB82_000621"
     CDS             9760..11175
                     /locus_tag="WIB82_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ3841958.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            11294..11803
                     /locus_tag="WIB82_000622"
     CDS             11294..11803
                     /locus_tag="WIB82_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3841959.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            11966..13348
                     /locus_tag="WIB82_000623"
     CDS             11966..13348
                     /locus_tag="WIB82_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ3841960.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMKIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAGIGLAFDGDADRLGLVTKDGNIIYPDRQLMLFAQDVLSRNPGAK
                     VIFDVKSTRLLAPWIKEHGGKAIMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQNISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLSWPL"
     gene            complement(13404..14159)
                     /locus_tag="WIB82_000624"
     CDS             complement(13404..14159)
                     /locus_tag="WIB82_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ3841961.1"
                     /translation="MIKIRNIHKTFGENTILHGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(14169..14885)
                     /locus_tag="WIB82_000625"
     CDS             complement(14169..14885)
                     /locus_tag="WIB82_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ3841962.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(14875..15702)
                     /locus_tag="WIB82_000626"
     CDS             complement(14875..15702)
                     /locus_tag="WIB82_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3841963.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            15987..16895
                     /locus_tag="WIB82_000627"
     CDS             15987..16895
                     /locus_tag="WIB82_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ3841964.1"
                     /translation="MSTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         4196 a         4891 c         4161 g         3692 t
ORIGIN      
        1 agcattaaaa aatgccgtct gaacgtcctt acgttttcag acggcattgc cttatgccca
       61 aataacggtt tcttccaagg gcctgcgcgc tttcgtggcg atttcctgaa cgcggtagcc
      121 gaatgtcgcg gcaacggaca cgccccattc tgccgcatcg aacaaaccga actgcccgga
      181 caatacgcgt tccatctcgg catagttgaa accttccacc gggcaggaat cgatacctgc
      241 catcgccgca cccgtcatca tgttgcccaa cgcgatatag gtctggcggc aacaccagtc
      301 aaacaaggcg cgcgaatcgt ccaaaatctt gatgtcgtcg gcttgaaacg cctgatacct
      361 cgccaaagat tttgccatgg catccggttc ggtaacgccg cgccgtttga ggctttccaa
      421 cataaacggg ctgtcgaagc gggcattttt ctttgccaaa aacaccacca aatgactggc
      481 ggtatccaaa gcatccgcca taccccaaga aaacggcttg attgcctgtc ggatttcggg
      541 gttttgaacc acaacaaact gccaaggctc cgaaccgacc gaactgggcg acaaacgccc
      601 gagttctaaa ataaactgaa aatcctcggc actgatttta cgtgccgcat cgtaatgccg
      661 gcatgatttg cggtttttaa atgcggatag aacctgctct ttgcttaata ctgccatcgc
      721 acatcctttc attacttgta ttgcaaattg atttcaaacc gatacggcac tgccgcacca
      781 tacaatccgt cctcgccatc aagcttcatc cggactttac ccatctctct acggctggga
      841 aaacaaagcg gcggcagact cgggaataac gccccaatgc ctttcagacg gcatttgggc
      901 atcattgcaa acgcgcctac gggtaggcga tataggcata agccgaatgg ttgtggatag
      961 actcgaaatt ctcgctctcg acaacgaaac tcttgatgcg tttgtcggcg atcagcgaag
     1021 tagcgacatc acgcaccata tcttccacga atttcgggtt ttcgtaggct ttttcggtaa
     1081 catatttttc atcggggcgt ttgagcaaac cgtagagttg gcagcttgcc tgcgcctcca
     1141 cataatcaat gacttcctcg ataccgactt ctgcatcggc agtcaggctg acggtaacat
     1201 gcgaacgctg gttgtgcgcg ccatattggg aaatttcttt ggaacacggg caaagcgagg
     1261 ttacgggaat catgaccttc atactgtagc cgtatgcccc gtctttcatt tcgcccgtga
     1321 gacagacatc ataatccagt aaggaccgaa taccggaaac cggcgcggtt ttcttgcgga
     1381 aaaacggaaa agaaacgctg attttgccgg cgcgggaatc taaaagcgcg accatttcgg
     1441 cagtcagcct gtgcaattgt gcaaaatcca aggcttcaga atgttgctcc atcaatgcga
     1501 caaaacgcga catatgcgtc cccttctgct cagcaggcag gtaaaccgtc atggtcagac
     1561 gggcaacggt ggactggatg ccttctgcag tttgcagggt aatcggaaag cgcaggtctt
     1621 tgataccgac ctgattgatc ggcagattgc gcaaatctcg gctggattgc acgtctgcaa
     1681 tagtgttcat gagttgtttt tccttaatat tcatgtaatt gttgagatgt gtgcatggta
     1741 tggcgtttcc gccggggaat ataccgtcaa tctgcaagtg cgagattata tcactcgctt
     1801 ttagcagctg cattgattgt ttctattttt cagatagtga taaattttca gatagtgata
     1861 aaatcttcga ttattcacat ctacataggc agagaccaaa tgaaattcgc cacgaaagcc
     1921 attcattcca gctacgattg cgacgaacac aaccgcgcgc tgatgccgcc gatttatcaa
     1981 aacagtatgt ttacgttgca cgagattggc gaaaatgtgc cttaccgtta ttcgcgcctg
     2041 agcaacccga cccgtcagat tttggaagac accgttgccg atttggaaca cggtgcggca
     2101 ggctttgcgt tttccagcgg tatggcgggg attgatgccg tatggcgtac cttcctgcaa
     2161 ccgggcgata ccatcgtcgc ggtcgccgat atttacggcg gcgcgtatga cttattggtc
     2221 gatgtttatc aaaaatgggg ggtaaatgtt gtttttgccg atttaggcaa tcccgacaat
     2281 ttggacgaat tgctcaaagc gcacaaggtc aaactggttt ggctggaaac gccgtccaat
     2341 ccgcttttac gcttggtaga catcaaagcc cttgccgcaa aagccaaagc gaccggcgcg
     2401 ctggtcggca tcgacaacac ctttgccacg ccgtatctgc aacagccgtt ggatatgggt
     2461 tgcgattttg tgttccattc cgctaccaaa tatttgtgcg gccattccga cgtattgatg
     2521 ggcatcgtcg ttgccaaaac caaagagctg gctcagcctt tgcacgatat gatggtgcat
     2581 accggtgcga ttgcaggccc aatggactgc tggctggtgt tgcgcggcat caaaacgctt
     2641 gccctgcgta tggaggcaca ctgcaaaaac gcactcgaaa tcgcgcgccg tctggaagac
     2701 catcctgcca ttgaaaaagt gttccatccc ggcctgccgt cgcacgaaca ttatgaactc
     2761 gcccaagcgc aaatgcctaa aggcatcggc ggcgtggtga cggtttatct caaaaacgac
     2821 acgcgcgaag cggcaaacag cgtgattaaa aacatgaaac tggtcaaaat ggcttccagc
     2881 ctcggcggcg tggaaagttt ggtcaaccat tgctattccc agtcccacag cggcgtgccg
     2941 cataatgtga aaatggaaat gggcatcaaa gtcggtctgc tgcgtttctc catcggcatc
     3001 gaagacgcgg acgatatttg gaacgatatt tccgccgcac tcgatacaac tttgtaaact
     3061 gtaaaaatgc cgtctgaaac catggtttca gacggcattt caattaaccc ggccgaaaat
     3121 caacgcttca acatctttgc cgcctcaata gcgtaatagg tcaaaatccc gtccgcaccc
     3181 gcacgtttga atgccagcag gctttccaaa accactttgc cgccgtccag ccagccgttg
     3241 gcaatcgctg cctgcaacat cgcgtattct cccgaaacct gataggcata agtcggtacg
     3301 ccgaactcgt ccttcacgcg gcggacaacg tccaaatacg gtaaaccggg cttcaccatc
     3361 accatatccg caccttcctg aatgtccaac gccacttcat tcagcgcctc atcggtattt
     3421 gccggatcca tctggtaggt tttcttgtcc gccttgccca aattgcccga actgcccacc
     3481 gcatcgcgga acggcccgta aaacgccgaa gcatatttgg cggaatacgc cataatccgc
     3541 gtgtggatat gcccggcatc ctccaacgcc tcgcgaatcg caccgatacg cccgtccatc
     3601 atatcggaag gggcaaccac ctgcgcgccc gcttcagcgt ggcacaaagc ctgcttgacc
     3661 aaaacctcta cggtttcatc gttcatcaca taaccgtttt cgtccgtcag cccgtcctga
     3721 ccgtgaaccg tataaggatc gagcgcgaca tccgtcataa tgcccagttc gggaaacctc
     3781 tcgcgcaggg cgcggacggc tgccggcacg agtccttcgg gattgtacgc ttcctctgca
     3841 cgctcggttt tgtttgccgt aaccacggga aagagtgcca acatcggaat accgagcttt
     3901 accgcctctt ccgccgtaaa cagcagcctg tccaaacttt gacgcttcac acccggcata
     3961 gaaggcacat cctcctcgcg cgccgacccc tccaatacga acaccggata aatcaaatca
     4021 tcggcggtca gcgtgtgttc gcgcatcagg cggcgtgaaa aatcgtccct gcgcatacgg
     4081 cgcatacgcg aagccggaac attgcggtaa ggaaactgca taagccctcc aatcatcctg
     4141 tcaacaattc aaaccatacg gaagccgccg caacttgcag acagcatcgg gcggcaatca
     4201 aaatccgttt tcaccggaaa gggtttcggc ccgccgaaat gccgcgccga aggacggcgt
     4261 cccgcccatt aaagaacaat agcaaaacaa cagcttcagg ctgtcagcct tcgatattct
     4321 tacgccacaa taccaaaagt ttcccgatat gctgaacagg ttgcgcgtca accgcctcac
     4381 ataaggcagt gcagatttcg atacgttcgg cacggtcgtc gccgaatacg cgcactttaa
     4441 tcagctcatg cgccgtcaat gccgcatcgg tttccttgat gaccgcgtcc gtcagaccct
     4501 gctgaccgac catcacaaca ggatggagat ggtgcgcgcg cgctttcagt tccaaaattt
     4561 ctttggtgtt caatttggta tccgtcattt tcctactgct tgaataaaaa gaataatgcg
     4621 gcattgtacg cgatttggca cgacatcggc aaaattaacg tacaatacgc ggtttccatt
     4681 ccgacccgcg aaccactatg gctgtacgtt ccaaatcctc aaaagcgtgg ctgcacgaac
     4741 acgtcaacga ccactacgtc catatggcgc aaaaagacgg ctaccgcgcc cgtgccgcat
     4801 acaaactttt ggaaatcaac gacaaagaca aattaatcaa acccggcacg gtacttgccg
     4861 acttgggcag cgcgccggga agctggtcgc aggttgccgc caagctggcc ggcccttccg
     4921 gcgcggtatt cgctttggac atcctaccca tggaagccat agggggcgtc tccttcattc
     4981 agggcgactt ccgcgagaac gacgtactgg cacaatttga aaccttgttg gacaaccgcc
     5041 cgctcgacct tgtaatttgc gatatggcgc ccaatatgtc gggaaacgcc gtaagcgatc
     5101 aggcacgcag cctctatctg tgcgaattgg cccttgactt tgcctcgcaa cacctgaaaa
     5161 caggcggcag ctttttagtc aaagtctttc agggagcagg ctatcaggaa tacatggcag
     5221 ccatgcgcga aattttcggc acggtgcaga cgcgcaaacc cgaagcctca cgcaatcgct
     5281 ccagtgagat ttatttattg ggcaaaaata aacgctgaca atacagacgg cgtgctttac
     5341 aatcatttcc gttttacacc tcaattatgg agccttgcta agtggggaac acctttaaat
     5401 caatccttgt ctgggtcgcc ttgggtatcg gcctgatggc tgcgttcaac gctttagacg
     5461 gtaaaaaaga agacaacggg caaatcgaat actctcagtt catccaacag gtcaacaacg
     5521 gcgaagtatc cggcgtcaac atcgaaggat ccgtcgtcag cggctacctg attaagggcg
     5581 agcgcaccga caaaagcact ttcttcacca acgcgccttt ggacgacaac ctaattaaaa
     5641 cactgctcga caaaaacgtc cgcgtaaaag taacgccgga agaaaaaccg agcgcgctgg
     5701 ctgccctgtt ttacagcctg ctgcccgtcc tgctgctgat tggcgcatgg ttctacttca
     5761 tgcgtatgca gacgggcggc ggcggaaaag gcggcgcatt ctcattcggt aaaagccgcg
     5821 cccgcctgct ggacaaagat gccaacaaag tgacctttgc cgatgtcgcc ggctgcgacg
     5881 aagccaaaga agaagtacag gaaatcgtcg attacctcaa agcgccgaac cgctatcaaa
     5941 gcctgggcgg gcgcgtgccg cgcggcatcc tgctggcggg cagcccgggt acgggtaaga
     6001 cgcttttggc gaaagcgatt gcaggcgaag ccggcgtgcc gttcttcagc atttcaggtt
     6061 ccgactttgt cgaaatgttc gtcggtgtcg gtgcgagccg cgtccgcgat atgttcgagc
     6121 aggcgaagaa aaacgccccc tgcatcatct ttatcgacga gattgacgca gtcggccgcc
     6181 aacgcggcgc aggtttgggc ggcggcaatg atgagcgcga gcaaacatta aaccaattgt
     6241 tggttgaaat ggacggtttt gagagcaatc agactgtaat tgtgattgcg gcaaccaacc
     6301 gccccgacgt actcgatcct gcgctgcaac gccccggccg tttcgaccgc caagtggttg
     6361 tccccctgcc ggacatccga gggcgcgaac agattttgaa cgtccattct aaaaaagtgc
     6421 ctttggacga atctgtggat ttattgtccc tcgcgcgcgg cacgccgggt ttttccggcg
     6481 cggatttggc gaacttggtc aacgaagccg ccctgtttgc cggccgccgc aataaagtca
     6541 aagtcgatca gagcgatttt gaagacgcca aagacaaaat ctatatgggt ccggaacgcc
     6601 gcagtatggt gatgcacgaa gacgaaaaac gtgcgacggc gtatcacgaa tccggacacg
     6661 cgattgttgc cgaaagcctg ccctttaccg accccgtcca caaagtaacc attatgccgc
     6721 gcggacgtgc gctgggtctg acttggcagc ttccggagcg cgaccgcatc agtatgtata
     6781 aagatcagat gttgagccag ctctccatcc tgttcggcgg acggattgcc gaagacatct
     6841 tcgtcggacg catctccacc ggcgcatcaa acgactttga acgcgcaacc caaatggcgc
     6901 gcgaaatggt aacgcgctac ggcatgagcg acaaaatggg cgtgatggtt tatgcggaaa
     6961 acgaaggcga agtcttcttg ggacgcagcg taacccgttc gcaaaacatt tccgagaaaa
     7021 cccagcagga catcgacgcg gaaatccgcc ggattttgga cgagcaatat caggttgcct
     7081 acaaaatcct cgatgaaaac cgcgacaaga tggaaacgat gtgcaaagcc ctgatggaat
     7141 gggaaaccat cgaccgcgat caggtactgg aaatcatggc gggcaaacaa cccagcccgc
     7201 ccaaggatta cagccacaac ctgcgcgaga atgcggacac ggcggaagat aacgcgccgc
     7261 acgccccggc tcgggaagaa accgaagcac ctgctccggc agacaccgct tcgacagagt
     7321 ccgagcagca gcctgaaaac aaggcttaac ttcccgaaca aacggcagcc cgcaagctgc
     7381 cgttttccca tcctgttttc agacggcatt ttccgctcaa tgccgtctga aacgccaaag
     7441 ccgtatcggt ttttgcgaat ctgcgatatg gcggtttaaa ttgaaatcag acgaggcaag
     7501 gctgtaccgg tttaaaatta aaccgctata atactgccct tcgaatacac gcccgcattg
     7561 gtgatgcccg tgaaaaaagt tgaaaaaaac atcttggtgc tgcacggcgc ggacaaaatg
     7621 ttcgagctgg tcgataaggt tgaggattac ccgcactttc tgccgtggta cagcaaaacc
     7681 gaggtcatcg ggcgtagcgg caacgaactg aaggcacggc tgtttatgga ctatatgcac
     7741 gttcgccaat cattcgccac gcacaaccgc aacattccgg gcagggaaat ccgtatggaa
     7801 ctgctcgagg gtccgttcaa aaccttacgt ggaacgtgga aatttatcga tttgggcgac
     7861 gatatgtgca aaatcgaatt caatttggaa tacgattttt ccaatgccgt tttgtccgcc
     7921 ttaatttccc ccgtcttcaa ccacctttcc gccacgctgg tcgaagcgtt cgtcaaagag
     7981 gcagaccgcc gttatgcttg aaattgagat tgtgtacggg ctgtctgata agcaggtttt
     8041 gaaaaatatg cagcttgccg agggaacaac cgtccgcgcc gccgcactgc aaagcggttt
     8101 ggacggcata tttgaagatt taaacctgca ttccgcgcct ttgggcattt tcggcaaagc
     8161 cgtcaaagac gacacgcccc tgcgcgacgg cgaccgcatc gaagtgtacc gcccgctgtt
     8221 gatcgacccc aaagaagcgc gccgcaaacg cgttcaaaat caagaagaat aaccatgccg
     8281 tctgaagcct tcagacagca ttcgacagaa acacggaaaa tatcatgtca aacaaaatca
     8341 aaatgcttgt cggattgggc aaccccggca aagaatacga acaaacccga cacaatgcag
     8401 gcttttggtt cctcgacgaa ctggcttgga agtggaaggc ttcatttaaa gaagaaaaaa
     8461 aattcttcgg cgaagtcgcc cgtgccgccc tgcccgacgg cgatgtctgg ctgctcaagc
     8521 cgaccacgtt catgaaccgt tccggacagg cggttgccgc ccttgcgcag ttctacaaaa
     8581 tcaaacccga agaaatcctc gtcgtccacg acgaactcga cattccctgc ggacggatta
     8641 aattcaaact cggcggcggc aacggcggac acaacggctt gaaagacatt caggcaaaac
     8701 tcggcacggc agactattac cgcctgcgcc tcggtatcga ccacccgggc gaccgcaacc
     8761 tcgtcgtcgg ctatgtcctg aacaaaccca gtacggaaca ccgccgacag attgacgatg
     8821 ccgtcgccaa atccctgcaa gccatacccg acatccttgc cggcaaatgg gaagaagcaa
     8881 cccgcttcct gcacagcaaa tgacccgatg ccgtctgaag ccctttcaga cggcatgttc
     8941 ccgatttcca tatccgaaca gtcatgaccg aactcaagca gcttatccaa accgaatcca
     9001 tccccgtcat cgaagaaacc ctcgatttcc tgctgtacga atgcagcatc gacgacgcac
     9061 catccgccga agaagtggca caatggcgcg acatacttgc cgcacgcggc ggcaaattcc
     9121 tgcgcctgtc caaaatctgc caaacgtggc tggacgagga ggcggcatga agctgccgcg
     9181 caaccgcttc agcctgcttt ccgcattgtg gtttgccggc ggcatctatt cgctgctctt
     9241 caaagctgcc gacaccgcgc cgccgccgtt tccgcatttc gacaaagcag cacaccttgc
     9301 cctgtttttc gcacaaatct ggcttttgac caaagcattc aaaaccggaa aacttcccat
     9361 cccctaccgc agcctgatgg tctttgccct ctgtttcgcc ctcttcagcg aatgcgcgca
     9421 ggcatggttt accgcaacga gaaccggcag tttgggcgat gttcttgccg atatggcagg
     9481 tacggttctc gcactctttg ccgcccgcgc cgccgaccgc ccggactgaa tcggtttatt
     9541 tcccccaaac aggatgcacc tcttcccgat acatcctatt tttcccctta catatcaatc
     9601 ccatctcata aacaaaaccg acaaatcgtt tacaatatat ttacactaca tcagattaca
     9661 aatatactcg acccagttgc agaatcggcg gtttcacaaa ccgtttcgga ttgcacgaca
     9721 caaaagaaaa accgatttta ttgccttaaa ggagtattca tgaacctgca tgcaaaggac
     9781 aaaacccagc atcccgaaaa cgtcgagctg ctcagtgcgc agaagccgat taccgacttt
     9841 aagggtctgc tgaccaccat tatttccgcc gtcgtctgtt tcggcattta ccacatcctg
     9901 ccttacagcc cggatgccaa taaaggtatc gcgctgctga ttttcgttgc cgcactttgg
     9961 tttaccgaag ccgtccacat taccgtaacc gcactgatgg tgccgattct cgccgtcgta
    10021 ctcggtttcc ccgacatgga catcaaaaag gcgatggctg atttttccaa cccgattatc
    10081 tacatttttt tcggcggctt cgcgcttgcc accgccctgc atatgcagcg gctggaccgt
    10141 aaaatcgccg tcagcctgtt gcgcctgtcg cgtggcaata tgaaagtggc ggttttgatg
    10201 ttgttcctcg ttaccgcctt tctgtccatg tggatcagca acaccgccac cgccgcgatg
    10261 atgctgcctc tagcaatggg tatgctgagc cacctcgacc aggaaaaaga acacaaaacc
    10321 tacgtcttcc tcctgctcgg catcgcctat tgcgccagca tcggcggctt gggcacgctc
    10381 gtcggctcgc cgcccaacct gattgccgcc aaagccctaa atctggactt cgtcggctgg
    10441 atgaagctcg gcctgccgat gatgctgttg attctgccct tgatgctgct ctccctgtac
    10501 gtcatcctca aacctaattt gaacgaacgc gtggaaatca aagccgaatc catcccttgg
    10561 acgctgcacc gcgtgatcgc gctgttgatt ttccttgcca cagccgccgc gtggatattc
    10621 agctccaaaa tcaaaaccgc cttcggcatt tccaatcccg acaccgttat cgccctgagt
    10681 gccgccgtcg ccgtcgtcgt cttcggcgtg gcgcaatgga aggaagtcgc ccgcaatacc
    10741 gactggggcg tgttgatgct cttcggcggc ggcatcagcc tgagcacgct gttgaaaaca
    10801 tccggcgcgt ccgaagcctt gggacagcag gttgccgcca ccttttccgg cgcgcccgca
    10861 tttttggtga tactcatcgt cgccgccttc attatttttc tgaccgagtt caccagcaac
    10921 accgcctccg ccgcattgct tgtaccgatt ttctccggca tcgctatgca gatggggctg
    10981 cccgaacaag tcttggtatt cgtcatcggc atcggcgcat cttgtgcctt catgctgccg
    11041 gttgccacac cgcctaacgc gattgtgttc ggcacgggct taatcaagca acgcgaaatg
    11101 atgaatgtcg gcatactgct gaacatcctc tgcgtagtat tggttgctct gtgggcttat
    11161 gctgtactga tgtaaaccat cgacctaaac aacaagaccg tctgaaagaa tatttttcag
    11221 acgaccttga agtttgtcgt tcagacacaa tttgtcgaat cattcaaaac cggattctaa
    11281 cgaaaggaaa cccatgatta tcctgcacac caacaaaggc gacatcaaaa tcgaactcga
    11341 tttcgacaaa gcccccgtta ccgccaaaaa cttcgagcaa tacgtcaaag acggcttcta
    11401 cgacggcgta atcttccacc gcgtcatcaa aggcttcatg attcaaggcg gcggcatgga
    11461 tgaaaacatg aacgaaaaag aaacccgcga tccgattcaa aacgaagcgt ccaacggcct
    11521 gcccaacgat aaatacacca tcgccatggc acgcacttcc gacccccatt ccgccagcgc
    11581 gcaattcttc atcaacactg ccgacaacgc tttcctgaac ttccgttcta aagagctgta
    11641 cggcaaaacc gtcgtccaag actggggcta tgcagtattc ggtaaagtcg ttgacggttt
    11701 tgacgttgtc gatgccatcg aaggcgtatc caccaaacgc cacggctacc atgacgacgt
    11761 accgagcgaa cctgtcgtca tcattaaagc cgaagcggta taaaccggca atccgaaagc
    11821 agcctgcaca aaggctgctt tttttgtttc aggcggcata aaatccaagc acatatgccg
    11881 tctgaacacc tcccgcaagc cttcggacaa tgttgcaacg tgttaaaata gcctcctttc
    11941 ctgaaaccac aacaaggaaa ccatcatggc aaatattgcc cgcgacatct tcaaagccta
    12001 cgacatccga ggcattgtcg gcaaaaccct gactgacgat gccgcctatc tcatcggcag
    12061 ggccatcgcc gccaaagccg ccgaaaaagg catgacccgc atcgcgctcg gacgcgacgg
    12121 acgcttgagc ggtcccgaac tgatggagca catccaacgc ggctttaccg acagcggcat
    12181 cgacgtcctc aacgtcggta tggtcgccac gcccatgctc tattttgccg ctatcaacga
    12241 atgcggcggc agcggcgtga tgattaccgg cagccacaat ccgcccgatt acaatggttt
    12301 caaaatgatg ctcggcggcg acacgctcgc aggcgaagcc attcaagaac ttttagccat
    12361 tattgaaaaa gacggttttg ttgccgccga caaacaaggc aacgtaaccg aaaaagacat
    12421 ctccggcgaa taccacaacc acatcgtcgg ccacatcaaa ctcaaacgcc ccatgaaaat
    12481 cgccatcgac gcaggcaacg gcgtaggcgg cgcattcgca ggcaaactct acaaaggctt
    12541 gggcaatgaa gttaccgaac ttttctgcga cgtggacggc aatttcccca accaccatcc
    12601 cgacccatcc aaacccaaaa acctgcaaga tttgattgcc gcgctgaaaa acggcgatgc
    12661 cggaatcggc ctggcgtttg acggcgatgc cgaccgcttg ggcctggtta ccaaagacgg
    12721 caacattatt tatcccgacc gccaactgat gctgttcgcg caagacgtgt taagccgcaa
    12781 tcccggcgcg aaagtcatct tcgacgtgaa atccacccgt ctgcttgctc cttggattaa
    12841 agaacacggc ggcaaagcca taatggaaaa aaccggccac agctttatca aatccgccat
    12901 gaaaaaaacc ggcgcgctgg ttgccggcga aatgagcgga cacatcttct tcaaagaacg
    12961 ctggttcggc ttcgacgacg gtctgtacgc cggcgcacgc ctcttggaaa tcctgtccgc
    13021 ctccgataat ccgtccgaag tgttgaacaa cctgccgcaa aacatttcca cgcccgaact
    13081 caacatcgcc ctgcccgaag gcagcaacgg ccatcaggtt atcgacgaac tcgccgccaa
    13141 agccgaattc gaaggcgcaa ccgaaatcat caccatcgac ggtctgcgcg ttgaatttcc
    13201 cgacggcttc ggtctgatgc gtgcttccaa taccacgccg attctggtgt tgcgttttga
    13261 ggcggatacg caagcagcca tcgagcggat tcaaaaccaa ttcaaagccg tcatcgaaag
    13321 caatccaaat ttaagctggc cgctgtaaac atcgaaaaaa tgccgtctga aggcttcaga
    13381 cggcattttt cacaaatagt taatcatatc ttggtagatt ggatttggct taaaaatctc
    13441 cgcgtccgtt cgtgtttggg gtgatcgaac aactctttcg ggctgccttg ttcgacaata
    13501 acgccgccgt ccatcacgac aacggtagtc gccacttcca gcgcaaactt gatttcgtgg
    13561 gtaacgacga ccatcgtcca accttcccgc gccaattcct tcatggcgtt caacacgtct
    13621 tgcaccaact cggggtcaag cgcggaagtg ggttcgtcaa acaacatcag ctcgggctga
    13681 atcgccaatg ctcgggcaat gccgacgcgc tgctgctgac cgccggaaag ctggtaggga
    13741 tacaaatcca ctttgtcgcc caagccgact ttttccagca gtttcagagc ctcttcgcgc
    13801 gcttgggcgg caggcttgcc ctgtacggca accggtcctt ccatcacgtt ttccaaggcg
    13861 gttttgtgcg gaaagaggtt gtattgttga aacaccatgc ctgatttgcg gcgcagtgcc
    13921 aaaatatcgt gtttgcttgg ttttttagaa aaatcgattt tcagcggtcg ctcgttgtcg
    13981 aactcgattt gtccgtcttc gggcatttcc aacgcgttta ggcagcgcag aaacgtcgtt
    14041 ttgcctgagc cggaaggccc gaggatgacg accacctgcc ctttgcacac atccaaatcg
    14101 atgccgtgca aaatggtatt ttcgccgaag gtcttatgga tattgcggat tttaatcatg
    14161 acaactcctt atttggcgac gtagcggtcg aaacgttttt ccaaacgcgc ctgaatcagg
    14221 aacagcactt tacaaaaaca ccagtaaacc aaagcggctt cgatatagac gggcaaaaag
    14281 tcataagtgc ggtttgccgt ttcctgcgcg acgcggaata attccgttac cgtcacgact
    14341 gccgcgagcg aggtgttttt aaacaaaccg ataaactcgt tgctcaaagg cggcacggca
    14401 acgcggaatg cctgcggcgc gacaatgcgg cggaacgtct gcatataggt catgccgatg
    14461 gagaaacctg cttcccattg gcctttcggt acggacaaaa ttgccgcgcg tatggtttcg
    14521 gaagcatatg cgccgacatt gagcgaaaag ccgatgatgg cggcaggaat cgggtcaata
    14581 tagatgccga cggaaggcag cccgtaaaac acaatcacaa gctgaaccaa cagcggcgta
    14641 ccgcgaatga cggaaatata aaattccacc aatttcagca ggattttccg cacgatgccg
    14701 ccggcgggca taatccgcac caaagccacg gctaccgcaa tcatcatacc gataacgaaa
    14761 gaagctgccg ccaaaggcag agagaccgcg aagccggctt tgaccatagg caaaaacgcg
    14821 ctgacaatca tatcggcgcg tgtttccgtc ataaacggca gcgaagcaag gaaattattg
    14881 aacactgatg tcttttccga agaattgttc gcccagtttt ttcagcgtac cgtcggcttt
    14941 cagctcgttg attgccgtac tgaatttcgc cacggcttcg tcattgccct tgttgacaat
    15001 caggccggaa ccgacttttt catcggcagg tgcggaccaa acgattttca cgcccgcatt
    15061 cgggtttttc ttcagatagt ccaaaaccgc caattcgtcg ttcagggttg catcggcacg
    15121 tttttgttca atcagggtca gcgattgcgc caaaccgtca acagccacca aatctgcgcc
    15181 tgcagctttg gctttttcgc cgtagttgct ggtcagggat tgtgcggttt tcacgccttt
    15241 gatgtcgtcg atagatttga tgttgctgtc gttacgggca accaatacgg caccgctcca
    15301 gctgtaagga tcggatttgt cgaatgtcgc ttggcgttcg gggctggtca gaccgacttg
    15361 gtttgccacc acgtcgaaac gccccgcctt caaacccgcc atcatcgaat cccattgcgt
    15421 ttctttaaac tcgactttca cgcccagttt ttccgccacg gcgcgggtta cttccacatc
    15481 gtaaccggtc agtttgccgt ccttgtcgtg gtaggtaaac ggtgcgtaag tgccttccgt
    15541 gccgacggta accgtgcctt tattgttgat gcgctcgatt aaagaaccgg aaactgccga
    15601 ttgtgcaggc gcggaagatg ctccgctgcc gccttccgaa ccgccgcagg ccgccaaaac
    15661 caatgcggca atgccgccga gtacgaattt tttcaacatc atcctctcct gtaaaaagtg
    15721 tgcaagtgcg gcattctaag tgaaattgcg gcaaacacaa agaatccttt ttttatatgg
    15781 ttattccaaa tcggaatgga aaagatatgc cgaagccgcc ggaagcggaa ataaaggggg
    15841 cgggaaaggc ggcgggcggc acgaaaccga aaagcggcat caggtatgca aacggcggat
    15901 tcacgcacgc cgttccggca aaaatccgcc tgccgattcg gtgctataat ccatccgaaa
    15961 cagatgacac caaaaaggaa catctcatga gcacgcccca atccgccatc attcccgacc
    16021 acgcccaagc cggcatcttt atcgaagccg acttcgccgc caaccgcctc aacgatatta
    16081 aagccgcctg ccgcgcttcg ctcgacgcgt taactgcctt gaaagcccgc tttccggacg
    16141 atattttggg tctgaccatc gccttcggca gcaaagcctg ggcaacattc ggacatacgg
    16201 acgaaggcag tgaaatcaaa cccttctccg aaatgggcaa cgggcttgcg ccgtccacgc
    16261 agcacgatat gtccatccac atccaatcct tccgccaaaa cgccgcctac gcgcttgccc
    16321 aatccgtttt gggcgcgttc ggcgacagca tatgcgtcgc gtccgaagaa cacggtttgc
    16381 gcctgtatca ggatcgcggg ctggacggtt tcgtcgacgg cacggaaaac ccgcagggcg
    16441 atgaaaatgt ccgcgaagtc gccatcatcc ccgaaggaaa acccgatgcg ggcggcagct
    16501 atgtcctgct gcaaaaatac ctgcacgatt tgaaaaaatg ggatgccgtc cccgtcgccg
    16561 aacaggaggc ctcggtcgga cgcggcaagg aaaccgacga cgaattcggc cgcgatgtcc
    16621 gcctgcccga ttcgcacctc ggccgcgtca acctgaaaga aaacggagtc ggcctgaaaa
    16681 tcgtccgccg cagcctgccc ttcggcaaaa tcagcggcga acacggtttg atgtttaccg
    16741 catactgccg cacgctgcac aatatcgaag cgcaattatt gagtatgttc ggcgatacgg
    16801 acggcaaaac cgacctgctg ctccgacatc tctccgccgc cgtttcgggc ggatactact
    16861 acgcgccgtc tgtcgaacgc ctgcaaaacc tctaagccgg acgatgccgt ctgaaaccat
    16921 ttttcagacg gcattttccc
//
LOCUS       DAWHRS010000027        16602 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWHRS010000027 DAWHRS010000000
VERSION     DAWHRS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.027"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            241..1305
                     /locus_tag="WIB82_000628"
     CDS             241..1305
                     /locus_tag="WIB82_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ3841965.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1446..1769
                     /locus_tag="WIB82_000629"
     CDS             1446..1769
                     /locus_tag="WIB82_000629"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ3841966.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1841..2164
                     /locus_tag="WIB82_000630"
     CDS             1841..2164
                     /locus_tag="WIB82_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ3841967.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2211..2414
                     /gene="tatA"
                     /locus_tag="WIB82_000631"
     CDS             2211..2414
                     /gene="tatA"
                     /locus_tag="WIB82_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ3841968.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDGVIEHKKDEDKA"
     gene            2418..3104
                     /gene="tatB"
                     /locus_tag="WIB82_000632"
     CDS             2418..3104
                     /gene="tatB"
                     /locus_tag="WIB82_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ3841969.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLLDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRPKHR
                     AKPKLRVRKS"
     gene            3119..3889
                     /gene="tatC"
                     /locus_tag="WIB82_000633"
     CDS             3119..3889
                     /gene="tatC"
                     /locus_tag="WIB82_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ3841970.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKHARPYVIVGAFVIAAVITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPAKT"
     gene            3942..4547
                     /gene="maf"
                     /locus_tag="WIB82_000634"
     CDS             3942..4547
                     /gene="maf"
                     /locus_tag="WIB82_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ3841971.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVVKLPADIDETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPHSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIGGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4563..4916
                     /locus_tag="WIB82_000635"
     CDS             4563..4916
                     /locus_tag="WIB82_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ3841972.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4906..6720
                     /locus_tag="WIB82_000636"
     CDS             4906..6720
                     /locus_tag="WIB82_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ3841973.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACKSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6910..7587
                     /locus_tag="WIB82_000637"
     CDS             6910..7587
                     /locus_tag="WIB82_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ3841974.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7601..9007
                     /locus_tag="WIB82_000638"
     CDS             7601..9007
                     /locus_tag="WIB82_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ3841975.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9125..11551
                     /locus_tag="WIB82_000639"
     CDS             9125..11551
                     /locus_tag="WIB82_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ3841976.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDNLSTAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISLITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HPKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLGPEARYTRFMTHTNELPSATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRIMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11596..11841
                     /gene="rpsP"
                     /locus_tag="WIB82_000640"
     CDS             11596..11841
                     /gene="rpsP"
                     /locus_tag="WIB82_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ3841977.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11857..12366
                     /gene="rimM"
                     /locus_tag="WIB82_000641"
     CDS             11857..12366
                     /gene="rimM"
                     /locus_tag="WIB82_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ3841978.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12366..13115
                     /gene="trmD"
                     /locus_tag="WIB82_000642"
     CDS             12366..13115
                     /gene="trmD"
                     /locus_tag="WIB82_000642"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ3841979.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKTQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13131..13496
                     /gene="rplS"
                     /locus_tag="WIB82_000643"
     CDS             13131..13496
                     /gene="rplS"
                     /locus_tag="WIB82_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ3841980.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13814..14539
                     /locus_tag="WIB82_000644"
     CDS             13814..14539
                     /locus_tag="WIB82_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ3841981.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMQRFADRQIAYLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14575..15450
                     /locus_tag="WIB82_000645"
     CDS             14575..15450
                     /locus_tag="WIB82_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ3841982.1"
                     /translation="MNPYDLLITPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15527..16459
                     /locus_tag="WIB82_000646"
     CDS             15527..16459
                     /locus_tag="WIB82_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ3841983.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHDHDHDHEGHHHNHGEFDPHVWNDPVLMSTYAQNVAEA
                     LIKADPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRY
                     HIEFIAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNV
                     SGKLYSDALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4324 a         5307 c         3649 g         3322 t
ORIGIN      
        1 ccgtactggt ttttgttaat ccactataaa tgccgtctga agccgattcg gttttcagac
       61 gacatcatta tttgacctga cgcaaacaga aagccttccg cgtgcttaaa cacatctctt
      121 gtagtaaaat atctaaacag attcaactgg aaacggtcag gcttcttcac gccattccca
      181 tttcaaccgc cgcctgccag attcagacgg tattcctact tgaaaacaag gagcgttaca
      241 atgaaagcag cacgttttta cgacaaaggc gacatccgca tcgaagacat ccccgaaccg
      301 accgtcgccc ccggcaccgt cggcatcaat gttgcctggt gcggcatctg cggtaccgac
      361 ctgcacgaat tcatggaagg cccgattttc attccgcctt gcggtcatcc gcacccgatt
      421 tccggcgagt ccgcacccgt cacgatggga cacgagttct ccggcgtggt ctatgccgtc
      481 ggcgaaggcg tggacgacat caaagtcggc caacacgtcg tggtcgagcc ttacatcgta
      541 cacgacgacg taccgaccgg cccgggcagc aactaccacc tctctaaaga catgaacttc
      601 atcggcttgg gcggctgcgg aggtggcttg tctgaaaaaa tcgctgtcaa acgccgttgg
      661 gtgcatccga tttccgacaa aatcccgttg gatcaagccg ctttgatcga accgctgtct
      721 gtcggacacc acgcctatgt acgcagcggc gcgaaagcag gcgacgtcgc attggtcggc
      781 ggcgcaggtc cgatcggttt gctgttggct gccgtgttga aagccaaagg catcaaagtc
      841 atcatcaccg agttgagtaa agcacgcaaa gacaaagcgc gcgaatccgg cgttgccgac
      901 tacatcctcg acccgtccga agtcgatgtt gtcgaagaag tgaaaaaact gaccaacggc
      961 gaaggcgtgg acgtggcatt cgaatgtacc agcgtgaaca aagtgttgga tactttggtc
     1021 gaagcctgca aacctgccgc caatttggtt atcgtatcca tctggagcca ccctgcaacc
     1081 gtcaacgtcc acagcgtcgt gatgaaagaa ctggatgtgc gcggcacgat tgcctactgt
     1141 aacgaccacg ccgaaaccat caaactggtc gaagaaggca aaatcaacct cgagcctttc
     1201 atcacccagc gcatcaagct ggacgaactg gtttccgaag gcttcgagcg tctaatccac
     1261 aacaacgaat ccgccgttaa aattattgtg agtccaaacc tgtaagcaaa ttacatttgc
     1321 aacagcacaa atgccgtctg aaacgctcag acggcatttt ccaacgaagc gcaacaggcg
     1381 tattgcccga gagcagccgt ttagcctatc attccgaaca aacagaaaca aacagaagga
     1441 aaatcatggg agattccgta ctatccgcca tccaacaaac catcacccag cgaaaatctg
     1501 ccaatccgtc cgaatcttac gtcgcacagc tcttgcacaa gggcgaggac aaaatcctaa
     1561 aaaaagtgat tgaagaggcg ggcgaagtgt tgatggcatc caaagacaaa aacccgtccc
     1621 acctggttta cgaagttgcc gacttatggt ttcacaccat gattcttctg acacaccacg
     1681 acctgaaggc ggaagacgta ttggacgaac ttgcgcgccg ccaaggtttg tcgggcttgg
     1741 ccgaaaaagc cgctcgcaca gaatcttgaa tttatattaa aatccgcact ttcccacatt
     1801 caatccgtct gaccgctgtt cagacggcaa cggagccgtt atggacaact gtattttctg
     1861 caaaatcgcc gccaaagaca ttccggcgca aaccgtctac gaagacggcg aaatggtttg
     1921 tttcaaagac atcaaccccg ccgctccggt tcatctgctg ctgattccca aagtccattt
     1981 cgattcgttg gcacacgccg cgcccgaaca tcagcccctt ttgggaaaaa tgatgctgaa
     2041 agttcccgaa atcgccaaag cggcaggact ggcagacggc ttcaaaaccc tgatcaatac
     2101 cggaaaaggc ggcggacaag aggtcttcca cctgcatata cacatcatgg gtacacccgt
     2161 ataaaccgtt atttcacaat caacccctaa tacttactta aggatacatc atgggcagtt
     2221 tttctctgac gcactggatt atcgtactga ttatcgtcgt tttgatattc ggcaccaaaa
     2281 aactgcgcaa cgtcggcaaa gacctcggcg gtgcggttca tgacttcaaa caggggctga
     2341 acgaaggtac agacggcaaa gaagcccaaa aagacggcgt aatcgaacac aaaaaagacg
     2401 aagacaaagc gtaatttatg tttgatttcg gtttgggcga gctggttttt gtcggcatta
     2461 tcgccctgat tgtcctcggc cccgaacgcc tgcccgaggc cgcccgcacc gccggacggc
     2521 tcatcggcag gctgcaacgc tttgtcggca gcgtcaaaca ggaatttgac acgcaaatcg
     2581 aactggaaga actaaggaag gccaagcagg aatttgaagc tgccgctgct caggttcgag
     2641 acagcctcaa agaaaccggt acggatatgg agggtaatct gcacgacatt tccgacggtc
     2701 tgaagccttg ggaaaaactg cccgaacagc gcacgcctgc tgatttcggt gtcgatgaaa
     2761 acggcaatcc ctttcccgat gcggcaaaca ccctattaga cggcatttcc gacgttatgc
     2821 cgtccgaacg ttcctacgct tccgccgaaa cccttgggga cagcgggcaa accggcagta
     2881 cagccgaacc cgcggaaacc gaccaagacc gcgcatggcg ggaatacctg actgcttctg
     2941 ccgccgcacc cgtcgtacag accgtcgaag tcagctatat cgatactgct gttgaaacgc
     3001 ctgttccgca caccacttcc ctgcgcaaac aggcaataag ccgcaaacgc ggtttgcgtc
     3061 cgaaacaccg cgccaaacct aaattgcgcg tccgtaaatc ataaagaggg caaatccggt
     3121 gtccgaaaca caaaacgaac aacccgtcca accgcttgta gagcatctca tcgagctgcg
     3181 ccgccgcctg atgtggacgg ttgtcggcat cttagtctgc tttttcggca tgatgccgtt
     3241 tgcccaacaa ctctatactt ttatcgccga cccgctgatg gcaaacctgc ccaaagacac
     3301 cagcatgatt gccaccgatg tcatcgcacc atttttcgtg ccggtcaaag ttaccctgat
     3361 ggcggcattt ttaatttcgc tgccgcatac gctctaccaa atctgggcat ttgtcgcgcc
     3421 cgcactctac caaaacgaaa aacgcctgat tacgccgctc gtcctctcca gcgtcagcct
     3481 gtttttcatc ggcatggcat ttgcctactt tttggttttc cccgtcattt tcaaattcct
     3541 tgccagcgtt acccctgtcg gtgtcaatat ggcgacagac atcgacaaat acctctcctt
     3601 catcttgggg atgtttgtcg cattcggtac aacgtttgaa gtccccattg tcgttatcct
     3661 gttaaccaaa attggtgtgg taacaaccgg acagcttaaa catgcccgtc cctatgttat
     3721 tgtcggtgct tttgtcattg ccgcagtcat cacaccgccc gacgttattt cacaaaccct
     3781 gcttgccatt ccactgattc tcttatacga agcaggtatt tggttcggac gcttcttcac
     3841 tccacgttca gaacaggatg gcgacatacg gccgcctgca aaaacctgac actatgccgt
     3901 ctgaagcccc gcctcatacc gccacagatt aaggaatacc tttgaatacc ctctatttag
     3961 gttcaaacag cccacgcaga atggaaatcc tgacgcagtt gggctatcgg gtcgtcaagc
     4021 tgcctgccga catcgacgaa acggtcagac agaacgaaga ccctgcccgt tacgttcaaa
     4081 ggatggcaga agaaaaaaac cgaaccgccc tgaccctctt ttgcgaaacc aacggcacaa
     4141 tgcccgattt ccccctgatt accgccgaca cctgcgtcgt ttcagacggc atcatattgg
     4201 gcaaacccca ctcccaagcc gaagcaatcg aatttttaaa ccgattgtcc ggcaaacaac
     4261 ataccgtcct gactgccgtc tgcattcatt atcgcggcaa tgcggaaaac cgcgtccaaa
     4321 ccaaccgcgt cgttttcaag cccctgagtt cggaagaaat ttccgcctat gtgcaaagcg
     4381 gcgaaccgat ggacaaagcc ggtgcctacg ccgtacaagg cataggcggc atctttatcc
     4441 aatctatcgg aggcagcttc agcggcatta tggggctgcc cgtttatgaa accgtttcga
     4501 tgttgcagga tttgggatac cgtaccccac ctttcatccg agcctaaccc gtaaagaccg
     4561 ccgtgaacag acaaaccgct tacctcctta cctctttcag cctgatcgca ctgataatcc
     4621 tgtccctttc ctgggaactg tggattgcac cgttacgccc gggcggctcg tggctcgcgc
     4681 tcaaagccct ccccctctgt ctgccgcttt caggcatctt gaaaaagaaa atctatacct
     4741 accaatacag ctccatgctg gttctgattt attttgccga agccgtcatg cgcctgttcg
     4801 acgcctatcc ggcagaacaa atttgcgccg cgctttccgc agtattcagc atcatcttct
     4861 tcatatcctg cctgtcgttc gtcaaacaat acaaggaaac aaacaatgcc cgctgaaacg
     4921 accgtatccg gcgcgcaccc cgccgccaaa ctgccgattt acatcctgcc ctgcttcctt
     4981 tggataggca tcgtcccctt taccttcgcg ctcaaactga aaccgtcgcc cgacttttac
     5041 cacgatgccg ccgccgcagc cggactgatt gtcctgttgt tcctcacggc aggaaaaaaa
     5101 ctgtttgatg tcaaaatccc acctatcagc ttccttctgt ttgcaatggc ggcgttttgg
     5161 tatcttcagg cacgcctgat gaacctgatt taccccggta tgaacgacat cgtctcttgg
     5221 attttcatct tgctcgccgt cagcgcgtgg gcctgcaaga gtttggtcgc acacttcgga
     5281 caagaacgca tcgtgaccct gtttgcctgg tcgctgctta tcggctccct gcttcaatcc
     5341 tgcatcgtcg tcatccagtt tgccggctgg gaagacaccc ccctgcttca aaacatcatc
     5401 gtttacagcg ggcaaggcgt aatcggacac atcgggcagc gcaacaacct cggacactac
     5461 ctcatgtggg gcatactcgc cgccgcctac ctcaacggac aacgaaaaat ccccgccgcc
     5521 ctcggcgcaa tctgcctgat tatgcagacc gccgttttag gtttggtcaa ttcgcgcacc
     5581 atcttgacct acatagccgc catcgccctc atccttccct tctggtattt ccgttcggac
     5641 aaatccaaca ggcggacgat gctcggcata gccgcagccg tattcctaac cgcgctgttc
     5701 caattttcca tgaacaccat tctggaaacc tttactggca tccgctacga aactgccgtc
     5761 gaacgcgtcg ccaacggcgg tttcacagac ttgccgcgcc aaatcgaatg gaataaagcc
     5821 cttgccgcct tccagtccgc cccgatattc gggcacggct ggaacagttt tgcccaacaa
     5881 accttcctca tcaatgccga acagcacaac atacacgaca acctcctcag caacttgttc
     5941 acccattccc acaacatcgt cctccaactc cttgcagaga tgggaatcag cggcacgctt
     6001 ctggttgccg caaccctgct gacgggcatt gccgggctgc tgaaacgccc cctgacccct
     6061 gcatcgcttt tcctgatctg cacgcttgcc gtcagtatgt gccacagtat gctcgaatat
     6121 cctttgtggt atgtctattt cctcatccct ttcggactga tgctcttcct gtcccccgca
     6181 gaggcttcag acggcatcgc cttcaaaaaa gccgccaatc tcggcatact gaccgcctcc
     6241 gccgccatat tcgcaggatt gttgcacttg gactggacat acacccggct ggttaacgcc
     6301 ttttcccccg ccactgacga cagtgccaaa accctcaacc ggaaaatcaa cgagttgcgc
     6361 tatatttccg caaacagtcc gatgctgtcc ttttatgccg acttctccct cgtaaacttc
     6421 gccctgccgg aataccccga aacccagact tgggcggaag aagcaaccct caaatcacta
     6481 aaataccgcc cccactccgc cacctaccgc atcgccctct acctgatgcg gcaaggcaaa
     6541 gttgcagaag caaaacaatg gatgcgggcg acacagtcct attaccccta cctgatgccc
     6601 cgatacgccg acgaaatccg caaactgccc gtatgggcgc cgctgctacc cgaactgctc
     6661 aaagactgca aagccttcgc cgccgcgccc ggtcatccgg aagcaaaacc ctgcaaatga
     6721 cccgcgccgg cggatgcgga taccgcccga aatgtaaagc tccatgcaag acattgcaaa
     6781 aaacagcaaa ccggtaggga aatacgctat caaaaaacat tgcagccgtg ttaagataaa
     6841 ccgtcaaaca atcttttcac gccccgcccg aaacagggtc ggggcatacc cttacgaaaa
     6901 ggaaacacca tgagccgcgt attactcgta gatgacgatg ccctgctaac cgaactgctg
     6961 accgaatacc tgagcgccga aggtctgaac gtccgcagcg ttcccgacgg ggaagcaggc
     7021 gtacaggaaa tcctgagcgg gcagtacgat gtagtcgtat tggattccat gatgcccaaa
     7081 atgaacggct tggatgtctt gaaaaacgta cgcgcccgaa gcaccgtccc catcatcatg
     7141 ctgaccgcca aaggcgacga catcgaccga atcatcggct tggaaatggg cgcggacgac
     7201 tatgtcccca aaccctgcac accacgcgaa ctcttggcac gcatcaatgc catcctgcgc
     7261 cgcgcacaac acagcggcga acagaacaac gcacccaaca gcatctccgt cagcgatgtc
     7321 gtcctgtatc ccgccaaacg ccaggcatcc gtcaaagaca tgccgctcga actgaccagc
     7381 accgaattca acctgctcga agtcctgatg cgccatgccg gacaggtagt cagcaaagaa
     7441 accctgtccg tcgaagcact cgaccgcaag ctggcaaaat tcgaccgcag tatcgacgta
     7501 cacatctcca gcatccgcca caagttgggc gatgcctctc tgattcaaac cgtacgcggc
     7561 ttgggctacc tgtttgtcaa aaactgaaat aaacagataa atgaaactgt tccaacgcat
     7621 cttcgccaca ttttgcgcgg ttatcgtctg tgcaatcttt gtggcgagtt tttctttctg
     7681 gctggtgcag aacacccttg ccgaaaacca gttcaaccaa cgccgcacca tcgaaaccac
     7741 tttgatgggc agcatcattt ccgcattccg ggcacgcggg gacgcgggtg cgcgcgaaat
     7801 cctgacggaa tggaaagaca gccccgtctc atcgggcgtg tacgttatac agggcgacga
     7861 gaaaaaagat atcctgaacc ggtatatcga cagctatacc atcgaacgcg cccggctttt
     7921 cgccgccgga cacccgcatt ccaacctcgt ccatatcgaa tacgaccgct tcggcgaaga
     7981 atacctgttc ttcaccaaag actgggacaa actccaagcc cgccgcctgc ccagccccct
     8041 gttgatcccc ggcctgccgc tcgccccgat ttggcacgaa ctcatcatat tgtccttcat
     8101 catcatcgtc ggactgctga tggcatatat cctcgccggc aacattgcca aacccatcag
     8161 aatcttaggc aacggcatgg acagggtggc aaacggagaa cttgaaaccc gtatctccca
     8221 acaggtcgac gaccgcgacg acgaattgtc ccatcttgcc atccaattcg acaaaatggt
     8281 ggaaaaactc gaaaaactcg ttgccaaaga acgccacctg ctccaccacg tttcccacga
     8341 aatgcgctcc ccgcttgccc gtatgcaggc aattgtcgga ctgattcagg cgcagcccca
     8401 aaaacaggag caatatctca aacggctgga aggcgaactg acccgcatgg atacgctggc
     8461 cggggaactg ttaaccctgt cccgtctcga aacttccaat atggctttgg aaaaagaaag
     8521 cctgaaactc ctgcccttcc tgggcaacct ggtagaagac aatcaaagca ttgcccagaa
     8581 aaacggacaa acggttaccc tgtctgccga cggaaaaatc cccgaaaaca caaccatcct
     8641 tgccaacgaa agctacctgt accgcgcctt cgacaacgtc atccgcaacg ccgtcaacta
     8701 cagtcccgaa ggcagcacca tcctgatcaa catcggacaa gaccacaaac actggataat
     8761 cgacgttacc gacaacggcc ccggcgtgga cgaaatgcag ctcccgcaca tcttcaccgc
     8821 tttctaccgt gcagactcca gtgcgaacaa acccggaaca ggactggggc ttgcattgac
     8881 ccaacatatt attgaacagc actgcggaaa aatcatcgcc gaaaacatca aaccgaacgg
     8941 tctgcggatg cgcttcatcc tgcccaagaa aaaaaccggt tccaaaacag aaaaaagtgc
     9001 gaactgacca taatgccgtc tgaaccggct tcagacggca ttgcacaaac agttacccca
     9061 aaacaaacac acagcgggtg catctccaac aatcacgcac ataccgtccg caaaggaata
     9121 tatcatgtcg gcacaaaccg atccgggcta ctttttcatg cccaaccaca ttattctgat
     9181 aggcgcgagc gaacgtccgt acagcctggg cgaacgcgta ctcagcaacc tgctgagtac
     9241 gccctttcaa ggaaaaatca cccccgtaaa cccgcgccac cacaccatag ccgggcttcc
     9301 cgcctacacc agcctcaaca aaatccccgg caatgcagac ctgattattg ccgttaccct
     9361 acccgacagt tacgacaccc tcttcaaaac ctgccgcaaa aagcagctcc gacacatcat
     9421 cctcatacag gactgggaca acctgtctac cgcagaactg cacaccgccg aaactgccat
     9481 ccgcaaacac cacggcaacg gactcaacat caccgcctgc accaccgcag gcatccaact
     9541 gccctcactc ggactcaaca tcagtaccca agacggatac gccgcaggcc ataccgccat
     9601 actgaccggc aatgccgccg tcagccgcca aatcgacaac atcctgaaca aactccgtca
     9661 aggcacatcc cgccacatca gcctgcatcc cggcatcagc ctcatcacat ccgccgattg
     9721 gctcaaccgc ttcggacaca gcctgcacac caaaaccgcc gtcctacacc acaaccctga
     9781 agaggatcag cgcaaactgt tcagcgcaat ccgccaattt acccgccata cgccgctgat
     9841 tctccacatc acctgccgca cgacagaaac cgaccgtgcc gtactgcact gcctcgcccg
     9901 acactgcaac ttcctcatca gtttcaacgc cgacgacctc gaagccgcac tgcgcgccca
     9961 actgtccgac cttccacccc tgtcccgact cgacatcctg tccgacacgc ctgccgaatg
    10021 gctgaacgcg cacgcgccaa aaaccctcgc cctccacttt cccaaccttc cccaccacat
    10081 ccgcaacgga cacctgaccg gcacacccac accctcaatc tgccacgaca tcgcctcacg
    10141 tcagcttgcc cgccccgaca cccaagccgt cctgaccatc ctcagcccct ccggacacga
    10201 ggattacaaa aaaacagcac gcgcccttat ccgcctgtcc gaacagaccg ccaaacccct
    10261 gctcgtcagc agcccctttt cagacggcat aacacatttc gacaccccca ctcaggcaat
    10321 ccgcaccctt tcctaccgca acaccgccgc cgccctgaaa caggcacagc tcgacattgc
    10381 accgccgcag ccatgccgtc tgaaaacacc gcaaccccaa aacatcaaaa aagccctcga
    10441 agccgccgac cccgccctaa tcgccgaagc cctgcacctc cccccctacc ggcacaccac
    10501 ccataacgcc gtccaattcc aattcggcag ccaccccctc tatggcgaca tcctgaccgc
    10561 acgctgcaac ggacaaacca ctgccgtact cccgccgttt accacgctcg acagccgcca
    10621 ccttgcccgc tttgccgaac tcgacggcac acaaaccctc gaccagttcc tgcacacact
    10681 gaccatcatt cccgaatacc gccaacacat tctcggcatc accctcaacc taaacggcgg
    10741 acaatacagc agcgatttca tcttaaagac acccgaaaca cacgacacgc ccaaacgcaa
    10801 gaacacaggc aaagccgccc aaaccctcga acatgccgcc gcaaaaatgc agagtgccgc
    10861 cgcatacctg aaacacaaaa acccgacagc cgccgaattt ctccgccaca caagcgaagc
    10921 cgccgcagaa ctgctcggca gcaaaaccga aaccggagca gccgtaccca acgtacttgc
    10981 cccctatccc gcagcacacc ccaaaacact gtccctaaaa aacaacacga ccgttaccat
    11041 tacccccctt ttgcccgaag acgcagaagc caaacagcag ttcgtccgca gcctcggtcc
    11101 cgaagcacgg tacacacgct tcatgaccca caccaacgaa ctgccctcag ccacgttggc
    11161 acgcctgtgc aaccccgatt accactgtga agccgcatgg acggcaaagg atgccgacag
    11221 caacatcgtc gccgtcgtcc gccacagccg cctgaatcgc aacgaatgcg aattcggcat
    11281 cacactggcg gaacatatgc gcggcagcgg actggcacag aaaatgatgg aactcatcat
    11341 ccaaaccgcc gcacagcaag gctaccggat tatgagtgcc gacattctca aaaccaatac
    11401 ccccatgatc aaacttgctg aaaaatcagg atttaccctc aaggaatcgg acaccgaaaa
    11461 aaacctgtac cgcgcatatc tgaaccttgc ggcagacaaa acaacagaaa aaacaaataa
    11521 aaacttgcgc accgaccaca aaataaccta aaatcggcag tttccatata tccgattttc
    11581 tcaaaaggac tcaaaatggt agttatccgt ttggcacgcg gcggctcgaa acaccgcccc
    11641 ttctacaacg tcatcgttac tgactcacgc agccgccgcg acggccgctt catcgaacgc
    11701 gtaggcttct acaaccccgt agccaacgaa aaacaagagc gcgtccgcct caatgcagac
    11761 cgcctgaacc actggattgc acaaggcgcg caagtcagcg actccgttgc aaaactgatt
    11821 aaagaacaaa aagccgccta atccgcgttt gccgccatga cagacactca aaaccgggta
    11881 gccatgggct acatcaaagg cgtattcggc ataaaaggct ggttgaaaat tgccgccaac
    11941 accgaatatt ccgacagcct tttggactac cccgagtggc atttggtcaa ggacggcaaa
    12001 accatcagcg ttacccttga agccggaaaa gtcgtcaaca gcgaactcca agtcaaattc
    12061 gaaggcataa acgaccgcga cttggcattc tcattgcgcg gttacaccat cgaaataccc
    12121 cgtgaagcat tcgccccgac agaagaagac gaatactact ggacagactt ggtcggcatg
    12181 accgtcgtca acaaagacca taccgtttta ggcaaggtaa gcaacctgat ggaaaccggc
    12241 gcaaacgacg tattgatgat tgacggagaa cacgggcaga ttctgattcc gttcgtttcc
    12301 caatatatcg aaaccgtcga taccggcagc aagaccatta ctgccgactg gggtttggac
    12361 tactgatgct tatccaggca gttaccattt tccccgaaat gttcgacagc attacccgct
    12421 acggcgtaac gggacgcgcg aacagacagg gaatctggca gtttgaagca gtcaatcccc
    12481 gaaagtttgc cgacaacaga ttgggctaca tcgacgaccg cccgttcggc ggcggcccgg
    12541 gaatgattat gatggctccg ccgcttcatg cggcaataga acacgccaaa acacaatcct
    12601 cccaagctgc aaaagtcatc tacctcagcc cccaagggaa accgctgaca caccaaaaag
    12661 cggtagaact ggcagaactt ccgcatctga ttctgctgtg cggacggtat gagggcatag
    12721 acgaaaggct tctgcaaagc agcgtcgatg aagaaatcag catcggagac ttcgttgttt
    12781 ccggcggaga gcttcccgcc atgatgctga tggatgcggt attgaggctc gtacccggcg
    12841 tattgggcga tatgcagtct gccgaacagg attcgttctc aagcggcatt ttggactgcc
    12901 cccactacac caaaccctta gaatttcaag gtatggctgt tccggaagta ttgcgttccg
    12961 gcaatcatgg cttgatagcg gaatggcggt tggaacaatc gctgcgccgc accttggagc
    13021 gcagacccga tcttttggaa aagcgcgttt taatcccaaa ggaatcccgc ctcttagaaa
    13081 ccatccggca agagcaacgg gaaatccaat cataatttag gaaaaaaaca atgaacctga
    13141 ttcaacagct cgagcaagaa gaaattgccc gcctgaacaa agaaatcccc gaattcgcac
    13201 cgggcgacac cgtagtcgta tccgtacgcg tcgtggaagg tacccgcagc cgtctgcaag
    13261 cctacgaagg cgttgtcatt gcacgccgca accgtggtct gaacagcaac ttcatcgtcc
    13321 gtaaaatttc cagcggcgaa ggtgtggaac gtactttcca actgtactcc cctaccgttg
    13381 agaaaatcga agtcaaacgc cgtggcgatg tccgccgtgc caaactttac tatctgcgcg
    13441 gtctgaccgg caaagctgca cgcattaaag aaaaattgcc tgcacgcaaa ggttaataag
    13501 cgatttcccc caatgccgtc tgaaccttca gacggcattt cttattgctg cgatgccggc
    13561 aagtcaacga tatgcctgcc cctcatccac cttgattaac aatcactccc gcctattcaa
    13621 gcataattta cagaccaaac gttatacagt atcatatcag ccatataacg ataaacgctt
    13681 taccgccttc cctttcaaaa ccgcaaatca tcatgacacc ttccctttta ctatcaggat
    13741 tgactttccg cctcatcctt gccctgattg ccgtatccct tttatggggc gtttactttt
    13801 gggcagtatc cgcatgagca tcattgtcga aaacctgacc gtcagctacc gccgccgacc
    13861 tgccgtgcac catgtggaca ttacttttga agaacatagt atgtgggcgg ttttcggtcc
    13921 caacggcgca gggaaatcca cctttctcaa atccttaatg ggattgcagc ctatcgatac
    13981 gggcagcatc cggctggacg gattgacccg tcagaacatt gcctaccttc cccagcagtc
    14041 cgatatcgac cgctcccagc ctatgaccgt tttcgacttg gcggcaatgg ggctatggta
    14101 tgaaatcggc tttttcaaag ggataaatac cgctcaaaaa caacgcgttc acgaagcctt
    14161 ggagcgcgtc ggaatgcaac ggtttgccga ccgtcagatt gcctatctct caaacggaca
    14221 atttcagcgt gtcctttttg cccgaatgct ggttcaaaat gccaaattcc tgctgctcga
    14281 cgaacccttc aatgccgttg atgcacggac aacctacgag cttctcgacg tattgcagaa
    14341 atgccattgc ggcggacacg ccatcatcgc cgtactgcac gattacgaac aagtccgtgc
    14401 ctactttccc aataccctgc tgctcgcccg cgaaaaaatt gcggcaggcg caaccgagac
    14461 cattctgaca gaaccctacc tcgcccaagc caacgccaaa atgcagcaac aggaaagccc
    14521 cgagtggtgc gcctcataaa tgccgtctga aaccatgtcc ccgtaccaaa cactatgaac
    14581 ccctacgacc tgcttatcac accctttgca gaattcgact ttatgcgcta cgccctcgca
    14641 tccgtcttct gcctgtccct cagtgccgca cccgtcggcg tattcctcgt catgcgccgt
    14701 atgagcctga taggcgacgc attgagccac gccgtcctgc ccggtgccgc cgtcggctac
    14761 atgtttgccg gcttaagcct gcccgccatg ggtttgggcg gcgtagccgc aggtatgctg
    14821 atggcactgc ttgccggact cgtcagccgc ttcaccaccc tgaaagaaga tgccaacttt
    14881 gccgcctttt atctcagcag cctcgccatc ggtgtagtcc tcgtcagcaa aaacggcagc
    14941 agcgtcgatt tgctccacct ccttttcggc tccgtacttg ccgtcgatat tcctgccctg
    15001 caactcatcg ccgccgtatc caccctcaca ctgcttaccc ttgccgtcat ctaccgcccg
    15061 ctcgtactcg aaagcatcga ccccctgttc ctcaaatccg tcggcggcaa aggcgggctt
    15121 tggcacgtcc tctttctcgt cctggtcgtc atgaacctcg tatccggctt tcaagccctc
    15181 ggcacactca tgtccgtcgg actcatgatg ctgccagcca ttaccgcccg cctatgggcg
    15241 aagcacatgg gcgcactcat cctcctatcc gttctgacag ccctgctgtg cggcttgagc
    15301 ggactgctta tttcctacca catcgaaatt ccctccggtc ccgccatcat cctctgttgc
    15361 agcgtccttt atctcttttc cgtcatactc ggcaaagaag gcggcattct gaccaaatgg
    15421 ctcaaaaacc accgccacca caccacctga ccccgcaagc cgcttgaaca aacaggcggc
    15481 ttgttttacc ataacagatg ttacgttata tcacaggaga acagttatga aacacctcaa
    15541 actcaccctt attgccgcat tgctaaccac cgccgcaact gccgcccccc tgcccgtcgt
    15601 aaccagcttc agcattttag gcgacgttgc caagcaaatc ggcggagagc gcgtatccat
    15661 acaaagtttg gtcggagcca accaagatac tcatgcctat cacatgacca gtggcgacat
    15721 taaaaaaatc cgcagtgcaa aactcgtcct gctcaacggc ttaggacttg aagccgccga
    15781 tgtacaacgt gccgtcaaac agagcaaagt atcctatgcc gaagcgacca aaggcatcca
    15841 acccctcaaa gccgaagaag aaggcggaca ccatcacgac cacgatcatg accacgatca
    15901 tgaccacgac cacgaaggac accaccacaa ccacggcgaa ttcgacccgc acgtctggaa
    15961 cgaccccgtc ctcatgtcca cctatgccca aaacgtcgcc gaagccctga taaaggccga
    16021 ccccgaaggc aaagtttatt atcaacaacg cttgggcaac taccaaatgc agcttaaaaa
    16081 actgcacagc gacgcacaag ccgcatttaa cgccgtccct gccgccaaac gcaaagtcct
    16141 gaccgggcac gatgcctttt cctatatggg caaacgttac catatcgaat tcatcgcccc
    16201 gcaaggcgtg agcagcgaag ccgagccttc agccaaacaa gtcgccgcca tcatccgaca
    16261 aatcaaacgc gaaggcatca aagccgtctt taccgaaaac atcaaagaca cccgcatggt
    16321 tgaccgcatc gccaaagaaa ccggtgtcaa cgtcagcggc aaactgtatt ccgacgcact
    16381 cggcaacgcg cccgccgaca cctacatcgg aatgtaccgc cacaacatca aagccttgac
    16441 caacgcgatg aagcaataaa accgtttgat tggtttgaat ttgaaaaggc aaaaggccgt
    16501 ctgaaaatta tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca
    16561 gatagtacgg taaggcgagg caacgctgta ctggtttttg tg
//
LOCUS       DAWHRS010000028        16522 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWHRS010000028 DAWHRS010000000
VERSION     DAWHRS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.028"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            42..647
                     /locus_tag="WIB82_000647"
     CDS             42..647
                     /locus_tag="WIB82_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ3841984.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            719..1111
                     /locus_tag="WIB82_000648"
     CDS             719..1111
                     /locus_tag="WIB82_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ3841985.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(1140..1289)
                     /locus_tag="WIB82_000649"
     CDS             complement(1140..1289)
                     /locus_tag="WIB82_000649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841986.1"
                     /translation="MPSEARIAGSDGIFNIPISNRQGQHGFDTPKRVLPINRFRLRPI
                     GQNAV"
     gene            complement(1310..1756)
                     /locus_tag="WIB82_000650"
     CDS             complement(1310..1756)
                     /locus_tag="WIB82_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ3841987.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTDAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VEAAVAETGAAGMADMGKVMGLLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(1888..2100)
                     /locus_tag="WIB82_000651"
     CDS             complement(1888..2100)
                     /locus_tag="WIB82_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ3841988.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(2389..4239)
                     /locus_tag="WIB82_000652"
     CDS             complement(2389..4239)
                     /locus_tag="WIB82_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ3841989.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            4616..5353
                     /locus_tag="WIB82_000653"
     CDS             4616..5353
                     /locus_tag="WIB82_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ3841990.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFTVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSDIGRELIRED"
     gene            5355..6041
                     /locus_tag="WIB82_000654"
     CDS             5355..6041
                     /locus_tag="WIB82_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ3841991.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NTLARKLDKR"
     gene            6200..7063
                     /locus_tag="WIB82_000655"
     CDS             6200..7063
                     /locus_tag="WIB82_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841992.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(7125..7694)
                     /locus_tag="WIB82_000656"
     CDS             complement(7125..7694)
                     /locus_tag="WIB82_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ3841993.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            7852..8712
                     /locus_tag="WIB82_000657"
     CDS             7852..8712
                     /locus_tag="WIB82_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ3841994.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            8847..9185
                     /locus_tag="WIB82_000658"
     CDS             8847..9185
                     /locus_tag="WIB82_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3841995.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGSC"
     gene            9553..9906
                     /locus_tag="WIB82_000659"
     CDS             9553..9906
                     /locus_tag="WIB82_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ3841996.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            9896..10762
                     /gene="atpB"
                     /locus_tag="WIB82_000660"
     CDS             9896..10762
                     /gene="atpB"
                     /locus_tag="WIB82_000660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ3841997.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            10819..11055
                     /gene="atpE"
                     /locus_tag="WIB82_000661"
     CDS             10819..11055
                     /gene="atpE"
                     /locus_tag="WIB82_000661"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ3841998.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            11126..11596
                     /locus_tag="WIB82_000662"
     CDS             11126..11596
                     /locus_tag="WIB82_000662"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ3841999.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            11601..12134
                     /locus_tag="WIB82_000663"
     CDS             11601..12134
                     /locus_tag="WIB82_000663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ3842000.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            12145..13692
                     /locus_tag="WIB82_000664"
     CDS             12145..13692
                     /locus_tag="WIB82_000664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ3842001.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            13717..14592
                     /gene="atpG"
                     /locus_tag="WIB82_000665"
     CDS             13717..14592
                     /gene="atpG"
                     /locus_tag="WIB82_000665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ3842002.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            14630..16027
                     /gene="atpD"
                     /locus_tag="WIB82_000666"
     CDS             14630..16027
                     /gene="atpD"
                     /locus_tag="WIB82_000666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ3842003.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            16038..16460
                     /locus_tag="WIB82_000667"
     CDS             16038..16460
                     /locus_tag="WIB82_000667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ3842004.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         3840 a         4331 c         4378 g         3973 t
ORIGIN      
        1 atcagccgtt ttgcaggcat cacacaggag cgacaattat tatgatgacc ctctattccg
       61 gcattacctg ccccttcagc caccgctgcc gcttcgtttt gtacgaaaaa ggtatggatt
      121 ttgaaatcaa agacgtcgat atttacaaca aacccgaaga cctcgccgtc atgaatccgt
      181 ataaccaagt tcccgtgctg gtcgagcgcg atttggtgct gcacgagtcc aatatcatca
      241 acgaatacat tgacgaacgc ttcccccatc cgcagctgat gcccggcgat cccgttatgc
      301 gcggtcgggg ccggctggtg ctgtaccgta tggaaaaaga attgttcaac cacgtccaag
      361 tgttagaaaa ccccgccgcc accaacaagg aacaggcaaa agcgcgcgaa gccatcggca
      421 acggtctgac catgcttgcc ccttcgttca gcaaaagcaa atacatcctc ggcgaagatt
      481 tttctatgat tgatgtcgcc cttgctccgc tgctgtggcg gctcgaccac tacgatgtca
      541 aactgggcaa aagtgccgcg ccgctgctca aatacgccga gcgcatcttc cagcgcgaag
      601 cctttatcga agcactgaca cccgccgaaa aagccatgcg caaataagtc cgaaatgctt
      661 gcaaaaccca ccgttttgca ggcattttcc tattttggcg tacaacacgg aacccattat
      721 gcccacttcc accaaaccct acatcctccg cgccctctgc gaatggtgca gcgacaacag
      781 cctcacaccg cacatccttg tctgggtcaa cgaacacacg cgcgtcccca tgcagtacgt
      841 ccgcgacaac gaaattatgc tcaacatcgg cgcgaccgcc acgcaaaacc ttcaaatcga
      901 caacgattgg atcagctttt ccgcccgctt cggcggacag gcgcacgata tatggatacc
      961 tgtcggacac gtcctcagcc ttttcgcacg ggagaccgga gaaggtatgg ggtttgagtt
     1021 ggaagcgtac cgccccgata cgccgcctga aaacacctct gccgaaaccg cgccccgacc
     1081 cgccaaaaaa ggcttgaaat tggtcaaata aatctatgcc gtctgaacgg aatcgtgttt
     1141 cagacggcat tttgtccgat ggggcgcaaa cggaatctgt ttatcggcaa aacccgtttc
     1201 ggcgtatcaa aaccgtgttg cccctgccga ttcgatattg ggatattgaa aatgccgtct
     1261 gaacctgcga tacgggcttc agacggcatt ttgtccgata ttcgggcaat caggcggtca
     1321 gcacggcttt caggattttg ttgacttcgc ccatgtcggc tttacctgcg aggcgggttt
     1381 tcagcagccc catgacttta cccatatccg ccatacctgc cgcgccggtt tcggcaacgg
     1441 ccgcttcgac ctcggtacgg atttcgccgg cggaaagcat ttggggaagg tagcggtgca
     1501 gtacctcgat ttcggcgttt tctttgtctg ccaagtcctg ccgccccgcg tcagtataga
     1561 ttttcgcgct gtctttgcgc tgtttgacca ttttggtcag gatggcggtg attttggcat
     1621 cgtcggcttc ggtgcgttcg tccacttcaa actgtttgac ggcggcgttg ataaggcgga
     1681 tggtgccgag ggaaacttgg tctttggcgc gcatagccgt tttcatgtct tcggtaaggc
     1741 ggattttcag gctcataatg ttctcgctgg gatgtcggat ggaaacggcg ggtttcgatg
     1801 ccgtctgaaa agcaaaacac accgcagagc aggtcttgcg gtgtgtcctc atatcacagg
     1861 gctgacctgt aatttgtact tgaatgttta gtacattttg ggcggcagtt gttggctgcg
     1921 caggcgtttt tgcaggcgtt ttacggctgc cgcttttttg cgtttgcgtt cggtagtcgg
     1981 tttttcgtag gcttcgcggg cgcgcagctc ggtcagcaga ccggtttttt ctacggcgcg
     2041 tttgaaacgg cgcatagcga cttcaaatgg ttcattctct tttacgcgga ttgcaggcat
     2101 tttatttcct ttaataaatt cggttgtttc atctgcccat catatcggtg gaaagggtag
     2161 ggcagacggt gttgaaggtt taccgtatcg ctgtgccttg cggcggcggg atacgcgccg
     2221 tgacggaaac atcacctcct aacgggtcgg cttgtgccgt gtcaagctgc ttgaaaaagc
     2281 aggaaaaaca attttcggat tgtcttatat ttatgattcg ccgtcaatgc cgttcgggat
     2341 aaaaatgccg tctgaaagac cgggcgggtt tcagacggca tcggtacgtc agcgggcggg
     2401 gacaatgccc atacgctgtt tgagcgggat gtgcggcgcg ccgaagaggg cggcgtagta
     2461 ggcggcattg gccatcactt ttttgacata gtcgcgcgtt tcggaaaacg ggatggtttc
     2521 ggcatatacc gcgccttcga ggggcgtgtc cgcctgccat cggcgcgccc tgccgggacc
     2581 ggcgttatag cctgcggtgg cgaggacttc gttgttttgc aggcggcgtt tggtgtccgc
     2641 catataccac gtccccatac ggatattgcc gtcggcggtg taaagttgtg cggcatccat
     2701 accgattttg ccggcgattt cgcgcgcggt ggcaggcata acctgcatca gcccctgcgc
     2761 gcctacgcgg gattgcgcgc ccataacgaa gcggctttcc tgacgaatca gcccgtaaac
     2821 ccacgccgga tcgacattaa cattttgcgc gtggcggatt accgtgtctt taaacggcga
     2881 aatgtagcgc aaggtgtagt tgagtttgcg gtcggtgcgt tccgcgctgt tgaccgccat
     2941 atcgtaaaaa ccgtggtcga acgcggtttg cgcggcggtc agcagcttgt cttcatcgaa
     3001 gccgcgtgtg gcgaaacgcc attccgcctg agcctgacgg cgcattttcg catcgccggc
     3061 ggttcggctg tttcggaaca gcaccagcgc gcgcttaatc gcgccgtctt ccgccatacg
     3121 gaggacgctg tttttgccgg catcgggcac attgttgcgc gtatcgattt tgcgccccaa
     3181 ctcttccccc gccagcaccg cataaaaatt cctgcccgtc gctgccgcct gtttgtaaag
     3241 tttttccgcc tcttgcgtgt tgcccgttgc ggcgcgactg cgtgccagcc agtagagcca
     3301 ggtcgggctt ttttgcagtt tttcgggcat atgcgagatt acggaagcca gctcgtccca
     3361 acgtcgggcg cgcaaggcgg cgcgggcgta ccactcgatt tggtcgtcgg tcagttggcg
     3421 gcggtcggca actttgccgt aatagtccaa ggcggcaggc acattgaggt tttgcgactg
     3481 ataatgcccc aatacgcccc acgcgaaact gcgttgttcg aggcttaaac cgctttccat
     3541 ttcggacagc agggcggcgg cattcggcga tttgcgtgct tctttgccga tgacgttcaa
     3601 cagggcgtat tcgcgcgaac cttgtgtacc gccgtcaaac gggctgccca atgcggcggc
     3661 aaggttgcgt gcgtctgtgg tttggcggcc ggccagcagt ccgcgcacgc gcctccaggc
     3721 gtcgttgccg tccaacaagc cggatgcggc tgcctgttcc aacagtttgg tgcagcccga
     3781 aggcagtttg cccgtatttt taaccagttc agcggcacgc gtatagtcgt tgcggctcga
     3841 atcggcgtag cattcgactt cttgggcgcg ccctgccggt tcgagtttgg cgtattcctg
     3901 tgcaaacagc gtccactgtc tgcgtgcgcc caaagacttc agccactcgt tgcggacatt
     3961 ttccgccatc gcgctgtcgc cggcgttttc caaataggcg gcgacggcgg catcgttttt
     4021 ctgtttcact gcatccagtg cggacgggta gccgccgtaa tctgccagcg tttttccttc
     4081 gggttcggca gggcgggtgg gaacgcttgc cgaaaggtcg gcagtttcta tattgtctgc
     4141 cggggtcttg ccggctggca gtgtgtttgt cgaagaacac gcggcaagca ctagggccgc
     4201 cagcagcggc agggaatgct tcatagaggg taggtacatt ggatttcctt aagaatcgga
     4261 accctgaacg gtcagggttg gaaaagacaa aatgccgtct gaacaggcgt ttgcatgaat
     4321 tatatgccga aactgcaccg cctttggaat gtttctgaca taatttatat ttttcaatca
     4381 gttgccgttt tggtgagaac cgctgccttt gcccgtttca gacggcattg tccgaaatgg
     4441 ttgcccgctt cctgccttat tgacaaaaaa acgctttccc gataatatcc tacgaaaatt
     4501 aacctgccga tttgacacag cttgcgggca taacagctaa agcgttccga caatttcagc
     4561 tttatcttcc gcgcccgttg tgtccgacat cgggctttgt tgtatgggaa agacaatgat
     4621 tattttggac aaggtttcca agcattacca aacgcgcgac aagacccgtt ttgccgccgt
     4681 cgagccgacc agcctcgaaa tccgcgacgg cgaaatcttc gggctgatgg gttattcggg
     4741 tgcaggcaaa tccaccctgt tgcgcctgat taacctgttg gaacgtcccg acagcggcaa
     4801 ggtcaacgtc tgcggacaag agctgaccgc gctcgatgcc gccgcattgc gtcaggctcg
     4861 gcagaatatc ggcatggtgt ttcagcagtt caacctgttg aacaaccgca ccgtcgccgg
     4921 caacgtcgcc ttcccattgg aaatcgccgg atggccgtct gaaaaaatca aagcgcgcgt
     4981 tgccgaatgt ctcgaaatcg tcggactgac cgaacgcgcc ggccactatc ccgcccagct
     5041 ttccggcggg cagaaacaac gtgtcggcat cgcccgcgcg ctcgcatcca agccgcaagt
     5101 catcctcgcc gacgaaccca cttccgccct cgatcccgcc accacgcgca gcgtcttgga
     5161 atgtttggaa gacatcaaca aacgcttcac cgtaaccatc gtcatcgtaa cccacgaaat
     5221 gagcgtcatc agacgactgt gcgaccgcgc cgccctcttg gacaaaggca aagtcgtcga
     5281 aatcgtcgaa gtacgcggca accaaatcca cgcccaatcc gacatcgggc gcgaactgat
     5341 tcgggaggac tgatatggca gacttaacat tccaacaagc cgtttccaac atcatcggta
     5401 tgaaaggcga aatcgtccgc gccttgggcg aaacctttgt gatggtcggg ctgtccacca
     5461 cgtttgccgt cattttcggc acgctcttgg gcgtattgct cttcgtaact tccagccgcc
     5521 agctgcatta caacaaaccg gtaaatttcc tgctcgacaa cttagtcaac ctgatgcgcg
     5581 ccttcccctt cgtcattttg atgattgcga tgatacccgc cacacgcgcc atcgtcggca
     5641 gcaccatcgg cccggttgcc gcctcgctgg tgttgagcgt gtcgggattg ttttattttg
     5701 cccgactggt ggaacaaaac ctgcgcgaag tccccaaagg cgtgattgaa gccgccgccg
     5761 cgatgggtgc gccgccggtt gccatcgtct gcaaagtcct cttgaacgaa gcgcgcgcgg
     5821 gcatggtttc cagcattacc gtgcttgcca tcgggctttt gtcatacagc gcggcggcag
     5881 ggatgatagg cggcggcggc ttgggcgacc tcgccatccg ctacggctac taccgctacc
     5941 aaaccgaagt catcgtcttc atcgtcgccc tcctcgtgct gctggtcatc ctgattcaaa
     6001 gcaccggcaa cacgttggcg cggaaactcg acaaacgtta aacccgaatg ccgtctgaac
     6061 gccaaaaccc ccaccgctat ccgaaaaatg ctataaaatt cccctgttcg cggcaaatgc
     6121 cgtctgaacg ccgaatccgg acggcaggac tccctgcccg tcatttttgt ttgaaaccgc
     6181 cacaacatca ggagaaaata tgaaaacctt cttcaaaacc ctttccgccg ccgcactcgc
     6241 gctcatcctc gccgcctgcg gcggtcaaaa agacagcgcg cccgccgcat ccgcttctgc
     6301 cgccgccgac aacggcgcgg agaaaaaaga aatcgtcttc ggcacgaccg tcggcgactt
     6361 cggcgatatg gtcaaagaac acatccaacc cgagctggag aaaaaaggct acaccgtcaa
     6421 actggtcgag tttaccgact atgtacgccc gaatctggca ttggctgagg gcgagttgga
     6481 catcaacgtc ttccaacaca aaccctatct tgacgacttc aaaaaagaac acaatctgga
     6541 catcaccgaa gtcttccaag tgccgaccgc gcctttggga ctgtacccgg gcaagctgaa
     6601 atcgctggaa gaagtcaaag acggcagcac cgtatccgcg cccaacgacc cgtccaactt
     6661 cgcccgcgtc ttggtgatgc tcgacgaact gggttggatc aaactcaaag acggcatcaa
     6721 tccgctgacc gcatccaaag cggacatcgc cgagaacctg aaaaacatca aaatcgtcga
     6781 gcttgaagcc gcgcaactgc cgcgtagccg cgccgacgtg gattttgccg tcgtcaacgg
     6841 caactacgcc ataagcagcg gcatgaagct gaccgaagcc ctgttccaag aaccgagctt
     6901 tgcctatgtc aactggtctg ccgtcaaaac cgccgacaaa gacagccaat ggcttaaaga
     6961 cgtaaccgag gcctataact ccgacgcgtt caaagcctac gcgcacaaac gcttcgaggg
     7021 ctacaaatcc cctgccgcat ggaatgaagg cgcagccaaa taaggcagtc gtataaaatg
     7081 atgccgtctg aaccgtatcc gtgttcagac ggcatttttg tcctttaatc cgccattccc
     7141 tgccattccg ccgaatccgg cgtatcgatt ccgaacagcg acaaagcgtg tgcgacactg
     7201 tgcgccacta tgtcgtccgc cgtctgcggt ttgcggtaca tcgcaggaac agggggaaac
     7261 accacgccgc ccatttccgt tacccgcttc atattgtcca aatgggcaag gttcagcggc
     7321 gtttcgcgca ccatcagcac cagccgccgc ctttccttca aaaccacatc cgccgcacgc
     7381 gtcaagaggt tgtcgccgaa gccgtgcgcg acagaggcaa gcgtccgcat cgaacagggg
     7441 gcgaccagca tcccgtccgt tttaaacgta ccgctggcaa tgcacgcccc gatattgccg
     7501 atcggatgca cgaagtccgc caaggcatat acctcatctc tcgcataagc cgtttccgaa
     7561 gcgcgcgcca tctccgcacc tttcgatacc acaaggtgcg tttcgatatc ttgcgcgcgt
     7621 aaaagttcca aagccttcac gccgtattgg aaaccgctcg ccccgctgat gccgattatc
     7681 aaacgccgta ccatcatccg cctttcccat aaaaccgcct gcaacggcaa accggctatt
     7741 atagtgaaaa aacagaaatc cgataaacac ggatacaaat tgtcggcaac acccaatatc
     7801 cgataaaata cccgatttaa catcctatct gaataggcac gggagggcgg tatggcaaaa
     7861 gtaaaaggcg gattggggcg cggtttggat tcgctgctcg ccaacggcgc agacaacagc
     7921 agcggcgacc gattgaccac ggttgcggtt aaagatatcc ggcccggccg ctatcaggcg
     7981 cgtgttcaaa tcgatgacga agccttgcag gagctggcag attcgattaa ggcgcaaggc
     8041 gtgatacagc ccgtcatcgt gcgcgaacac ggactgtccc gatacgaact gattgcgggg
     8101 gagcggcgtt ggcgtgccgc acagattgcc ggcctgaccg aaatccccgc cgttatcaaa
     8161 accatcagcg acgaaaccgc gttggcaatg ggtttgatcg aaaacctcca gcgcgaaaac
     8221 ctcaacccca tcgaagaagc acaaggcttg aaacgccttg ccgacgagtt cggactgacc
     8281 cacgaaacca tcgcccaagc cgtcggtaaa agccgaagcg cgatttccaa cagcctgcgc
     8341 cttttaagcc tgcccgaacc cgtgcaggaa atgctttacc aacgccgcct cgaaatgggg
     8401 cacgcccgcg cactgctgac cctgcccgtc gtcgaacagc tcgagttggc gcaaaaggcc
     8461 gtcaaaaacg gctggtcggt gcgcgaagtc gagcgccgca gccaagccgc ccttcaaaac
     8521 aaacgtcccg agcccaaaaa aactgccgcc gccgacatcg gccgcctgaa tgatttgctg
     8581 actgaaaaac tgggtgtcaa cgccgaaatc aaaaccgcca accataaaaa aggcagaatc
     8641 atcctgcatt tcgacacgcc cgaaaccttc gaccatatct tgaagcagtt gggcatagat
     8701 taccgacctt aattttgcgg gatataccgt ctgaaatata gagaatagct ttccagattg
     8761 taagtgggaa atataattct attgacattt ttctgcttca cgtaagaatc gttttcctgt
     8821 tttcattttt aattttcgag gaaattatga acacacgcat catcgtttcg gctgcgttcg
     8881 ttgcgttggc attagcaggt tgcggctcaa tcaataatgt aaccgtttcc gaccagaaac
     8941 ttcaggaacg tgccgcgttt gccttgggcg tcagcccaaa tgccgtaaaa atcagcaacc
     9001 gcagcaatga aggcatacgc atcaacttta ccgcaactgt gggtaagcgc gtgagccaat
     9061 gctatgttac cagtgtaatc agcacaatcg gcgttaccac ttccgatgca atttgtttgg
     9121 gaggcggaac gcacaaaggc aaaagtcaat gcaatgcttt gcttaaagcg gcaggcagtt
     9181 gctaatcctt tattcggaaa aggtcgtctg aaaatatttt cagacgatct tttatttatt
     9241 gagcaaattc cccaaactgc cgacaatccg accgataaac tgctacaatt tcgcgttgat
     9301 tttttattat ttccacgtaa agcaaacaaa tcacgtaaga acattaaaaa tgtaaagaaa
     9361 ttgcggacaa actccttaat atcctgtaat tatatgtaat gtgtgttaga atgacacttg
     9421 tcgccgttat tcaggcggcg agcgcggcgg attgggatac gttgtttcac aaagattaac
     9481 tgtcgcttga cgtaataagg cttctaaatt atattagccg cgccccaaat ccgaggcgga
     9541 aacattccct ttatgaagca gattatcatc ctacaatccg ccgtattgtc catatgcgcc
     9601 gcagttgcct ttgccgtatg gggttttgcc ggattccttt cggcggtcgg tggcggtttg
     9661 tcctacctgc ttcccacgtt tgttgcagtt ttacttttaa aacttttcag gggaaacccc
     9721 ttcctgcaaa gcaggatgtt cgtcttcgga gagattttaa aagtagtgct gtcgctgttg
     9781 tccatgctcg ccgtatttgc aatatggcat caatcgctgg tgttcgcccc gtttctgatg
     9841 gggctgctcg gtgtcagcca tttggttttt ttagtattgt tgagagtgaa agattatggc
     9901 aggtgaaacc attaccgctg ccgactacat caagcaccac ttgcagagct tgaccagttt
     9961 gtcggatgtt actcagggtc agggactgaa aaacattgct gatttttcgt ttattaacct
    10021 tgatgccgtc ttttttgccg tcctgttggg cgtaatcggc agcttcctgt tgtggcgcgg
    10081 tgcaaaaaaa gcgacggcag gcgttcccgg acgtttccag gccgccgtgg aaatcttgtt
    10141 tgagtttgtg gacgatatgt gtaagagcat cattcacaac gaaaagtcta gaaaagccgt
    10201 cgcgccgttg ggtctgacgc tgtttgtctg gattttcctg atgaacgcga tggatatgct
    10261 gccggtcgat ttgctgccga tggtatggca gggcattacc ggcaaccatc acgccctgtt
    10321 gcgcgtcgta ccgactgccg atttgaacac cactttggca ctcgctgtcg gcgtgttgct
    10381 gatttgtatc tattacaaca tcaaaatcaa aggattgggc ggatggtttc acgagttgtt
    10441 cagcgcgccg ttcggtgcaa aactcgcacc tgcgaacttc ctgctgaatt tggtcgagtt
    10501 tctttctaaa accgtatccc acggtatgcg gttgttcggc aatatgtatg caggcgagtt
    10561 ggtattcttg ttgatagcct tgcttggcgg tgcttgggcg gcttccggca gcgtcgaagt
    10621 catggatccg attctgtttg tattccacat tattgccggt ttggcatggg cgattttcca
    10681 tattttggtg attaccctgc aggcgtttat tttcatggcg ttggcgttcg tctatatcgg
    10741 acaggcacat gatgcgcact gattttccta agtagtttgt ttttgtagtt taacctttgt
    10801 ttctttaagg agtttaaaat gggtttgatt gctatcgcat gtggtttgat cgttgcattg
    10861 ggtgcattgg gtgcctctat cggtatcgca atggtcggtt ctaaatattt ggagtcttct
    10921 gcccgtcagc ccgaactgat cggtccgctg caaaccaaac tgttcctgat tgccggtttg
    10981 attgatgccg cattcttgat tggtgtcgcc attgcactgc tgttcgcctt cgttaacccg
    11041 tttgcaggtg cataatcagg acggattcgt ccgttccgta caggcgcagc ctgtttgatt
    11101 aaccccagta cgaaggttaa gtaacgtgaa tatcaatgca acattattcg ctcaaatcat
    11161 cgtctttttc ggtttggtat ggtttaccat gaaattcgtg tggccgccga ttgcaaaagc
    11221 tttggatgag cgtgccgcaa aagtcgccga aggcttggct gccgccgagc gtggtaaaag
    11281 cgatttcgag caggctgaaa aaaaggttgc agaactcttg gcagaagggc gtaatcaggt
    11341 ttccgaaatg gttgccaacg ccgaaaaacg tgccgccaaa attgtcgaag aagccaaaga
    11401 acaggcttct tccgaggcgg cgcgcattgc agctcaggca aaggccgatg tggagcagga
    11461 attgttccgc gcacgcgaat ccctgcgcga gcaggttgcc gtgttggctg tcaaaggtgc
    11521 cgaatctatt ttgcgcagcg aagtcgatgc ttccaaacac gcaaaactgc tcgataccct
    11581 gaaacaggag ctgtaatctt atggcagagt tcgcaacgat tgccagacct tatgcaaagg
    11641 cattgttcgg tctggctcag gaaaaaaacc aaattgagtc ttggttgggc ggactggaaa
    11701 aacttgcggc ggttgttcag gaagggaagg tggcttcatt gattgaccgt cctgaaacga
    11761 atgcttcaga aaaagcagat atcctcatcg atttggtcgg tttgaaagac aaggagttga
    11821 aaaactttgt tatcgtcttg gccgggcaga aacgtttgtc gatattgccg gaagtatatg
    11881 ctcaatatca agacttgacc ttatcattca accatatcaa atctgccgtc atttacagtg
    11941 cctatccgtt gaccgacaaa caggtcggcg agttggcgca aatgctgaat aagcgtttcg
    12001 acagcgagct gaaaatctct gtcgaaatcg aaccggagct gattggcggc ataaaagttg
    12061 aagtgggtga tcaggttttg gatttgtctg tacaaggcaa attgagtgcc ttgtacacga
    12121 ctatgacgaa ttaggagagt tttcatgcag cttaatcctg ctgaaattag cgatttgatt
    12181 aaagccaaga tcgaaaatct gtctgtaaat gccgaagtgc gtactcgtgg tacggtgatt
    12241 tcggtaactg acggtatcgt tcgcatccat ggtttgtcag atgcaatgca aggtgagatg
    12301 ctcgaattcc cgggtaacac tttcggcttg gcgatgaacc tggagcgcga ctccgtcggc
    12361 gccgtagtgt tgggcgagta cgaacatatt aaagaaggcg acacggttac ctgtaccggt
    12421 cgtatcttgg aagtgccggt cggacgcgaa ctggtcggac gcgtcgttga tgcattgggt
    12481 cggcctatcg acggcaaagg cccgattaat acaactttga ccgcccctat cgaaaaaatc
    12541 gcaccgggcg tgattgcgcg caaatcggtt gaccagccga tgcaaaccgg tctgaaggcg
    12601 attgactcta tggttcctgt cggtcgcggt cagcgtgagt tgattattgg cgaccgtcaa
    12661 accggtaaaa cggccgtagc attggatgcc atcgtcaacc aaaaaggtac gggtgttatc
    12721 tgtatctatg tcgctatcgg tcaaaaagca tcttctattg ccaacgtggt ccgcaaattg
    12781 gaagagcatg gcgcgatgga acacacgatt gtggttgctg caactgcatc tgaagcggcg
    12841 gcattgcaat atatcgcacc ttactccggt tgtacgatgg gtgaattctt ccgcgatcgt
    12901 ggcgaagatg ccttgattgt ttatgacgat ttgtccaaac aggctgtggc ttaccgtcaa
    12961 atttccctgc ttttgcgccg tccgcccggc cgcgaagcct atcctggcga tgtgttctac
    13021 ctgcactccc gtctgttgga acgtgcggca cgtgtcaatg aacacgaagt ggagaaattg
    13081 accaacggcg aagtaaaagg caaaaccggt tctctgaccg cgttgccgat tatcgaaacc
    13141 caagcgggcg acgtatctgc tttcgttccg actaacgtca tttcgattac cgacggtcag
    13201 attttcttgg aaaccgacct cttcaacgcc ggtatccgtc ctgcaatcaa tgccggtatt
    13261 tccgtatccc gcgtaggcgg tgctgcacaa accaaagtga ttaaaaagct gggtggcggt
    13321 atccgtttgg cgttggcaca atatcgtgaa ttggcggcgt tctcgcaatt tgcatccgat
    13381 ttggatgaag ctacgcgcaa acaattggag catggcgaag tcgtaaccga actgatgaaa
    13441 cagaaacagt tcagtacgtt gaatacggct gaaatggctt tgaccctttg ggcaatcaac
    13501 aacggttcgt attctgatgt tccggttgct aaagctctgg cttttgaatc tgaatttttg
    13561 agctttgtcc gtacccaaca tccggaagtt ttggaagccg tcaatgcttc aggtgcaatg
    13621 tccgacgaga gcgagaaaac gcttgaagca gccatgaaat ccttcaaatc ttcttacgcc
    13681 tatcaggcat aaggcttgaa gtgaaaggag tctgaaatgg cagtaggaaa agagattctc
    13741 accaaaatcc gcagtgttca gaatacccaa aagatcacta aagcgatgca gatggtgtca
    13801 acctctaaaa tgcggaagac tcaggaacgg atgcgtttgg cgcgtccgta tgccgaaaaa
    13861 gtgcgtatgg tgatgagcca tcttgcgcaa accaataccg atcatggtat tccgttactg
    13921 gaatctcatc gggaaatcag acgtgtcggt tttattttga ttacgtctga taagggtttg
    13981 tgtggcggtt tgaacgccaa cgtgctgaaa aagtttttgg cacaagttca agagtatcgg
    14041 aatcaaggta ttgaagagga aatcgtatgc cttggcagta aaggtctgat ggcgtgtcag
    14101 agcattggtc tgaatgtggt tgccagtgcc gtaaatttgg gcgatacccc aaaaatggaa
    14161 atgctgctcg gacctttgac agaactcttc caacggtatg agaaacatga aattgacaga
    14221 atccatctgg tgtattcggg ttttgtcaat accatgcgtc aagaaccgag aatggaagta
    14281 ttgctgccta tcggtgagaa cgtgattggc gattcagctc ccaaatcacc gttcagctgg
    14341 gaataccgct acgaaccgac tgcacttgca gtgttggaat atctggttcg ccgctattta
    14401 gagtctgtgg tttatcaggc gttgagcgac aatatggcat ccgaacaggc agcccgcatg
    14461 gttgccatga aagctgcaac agacaatgca ggcaatgcca tcaaagagtt gcgtttggta
    14521 tataacaaat cgcgtcaagc tgcgattacc acggaattgt cagaaattgt agcaggtgcg
    14581 gcggctgtct gatgccgtct gaatcctgta cggaaattag gatacgataa tgagccaagg
    14641 caaaatcgta caaattatcg gtgcggttgt tgacgtggaa tttccacgcg acatgattcc
    14701 gcgcgtttac gacgctttga aattagacga aaacggtctg actttggaag tccaacagct
    14761 tttgggcgac ggcgtagtcc gtaccattgc gatgggcagc tcggacggtt tgaaacgcgg
    14821 catgactgtg agcaataccg gtgcgcccat tactgtgccg gtaggtaaag gtacgttggg
    14881 acgcattgtc gatgtattgg gaacgcctgt tgacgaggca ggtccaatcg ataccgacaa
    14941 gagccgtgcc atccaccaag ccgctcctaa gtttgacgaa ctgtcttcca caaccgaatt
    15001 gctcgaaacg ggcattaaag tgattgactt gctgtgtccg tttgccaaag gcggtaaagt
    15061 aggtctgttc ggcggtgccg gtgtgggtaa aaccgtgaac atgatggaat tgatcaacaa
    15121 catcgccaaa gcgcacagcg gcttgtccgt gttcgcaggc gtgggtgagc gtacccgcga
    15181 aggtaacgac ttctaccacg agatgaaaga ttccaacgta ttggataaag tggcaatggt
    15241 ttacggtcag atgaacgaac ctccgggcaa ccgtttgcgc gtcgcattga ccggtttgac
    15301 catggcggaa tacttccgtg acgaaaaaga cgaaaacggc aaaggccgcg acgtattgtt
    15361 cttcgtggac aacatctacc gttacactct ggctggtacc gaagtatccg cattgttggg
    15421 ccgtatgccg tctgcagtgg gctaccaacc gacattggca gaagaaatgg gtcgtttgca
    15481 ggagcgtatt acctctaccc aaaccggttc tattacttct atccaagccg tatatgtacc
    15541 tgcggacgac ttgaccgacc cgtctccggc aacaacgttc gcccacttgg acgcaaccgt
    15601 cgtattgagc cgcgatattg cctctttggg tatttacccg gcagtcgatc cgcttgattc
    15661 cacttcgcgc caactggatc cgatggtatt gggtcaagag cactacgacg tggcgcgcgg
    15721 cgtacagtcc accctgcaga aatacaaaga attgcgcgac atcatcgcca ttttgggtat
    15781 ggacgaattg tctgatgaag acaaactgac cgtaatgcgt gcgcgtaaaa tccaacgctt
    15841 cctgtcccaa ccgttccacg ttgccgaagt gtttacaggt tctccgggca aatatgtcgc
    15901 cctgcgcgat accattgccg gcttcaaagc catcttgaac ggcgaatacg accatctgcc
    15961 cgaacaggca ttctacatgg tcggcagcat cgaagaagcg gttgagaaag cgaaaacctt
    16021 aaactgagga ggtcggcatg agcatcatgc aagttgaggt ggtcagtagc gagcaaaaca
    16081 tctattcggg cgaagccagc tttgtagtgg ttccgaccgt tcaaggtgag cttggtattt
    16141 acccgcgaca cgagccgatt atgagtttgg tgcggccggg ggctttgcgt ttgaccgttc
    16201 cgggcgagga taaagaggtt ttggttgctg tttccggcgg tgttttggaa gtacagcctg
    16261 ataaagtaac tgtcttggcg gatgttgccg tccgcagtgc ggagatggat caggcacgtg
    16321 cggaagaggc gaaaaaagcc gcagaagcgg gcatttccca agctaaagac gataaggctt
    16381 tggcggaggc gcataaagca ttggctgctg cgattgcaca gctcaaaact ttggactata
    16441 tccgttcgca caagaaataa acttgtttgg tttggaaaag cacgaccgtt tggtcgtgct
    16501 ttttattttt ccaagttttg aa
//
LOCUS       DAWHRS010000029        16268 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWHRS010000029 DAWHRS010000000
VERSION     DAWHRS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.029"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            6..81
                     /locus_tag="WIB82_000668"
     tRNA            6..81
                     /locus_tag="WIB82_000668"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="WIB82_000669"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="WIB82_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ3842005.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="WIB82_000670"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="WIB82_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ3842006.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="WIB82_000671"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="WIB82_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ3842007.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="WIB82_000672"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="WIB82_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ3842008.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2472..2972
                     /gene="rplJ"
                     /locus_tag="WIB82_000673"
     CDS             2472..2972
                     /gene="rplJ"
                     /locus_tag="WIB82_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ3842009.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3030..3401
                     /gene="rplL"
                     /locus_tag="WIB82_000674"
     CDS             3030..3401
                     /gene="rplL"
                     /locus_tag="WIB82_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ3842010.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3591..7769
                     /gene="rpoB"
                     /locus_tag="WIB82_000675"
     CDS             3591..7769
                     /gene="rpoB"
                     /locus_tag="WIB82_000675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ3842011.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAAEKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7926..12101
                     /gene="rpoC"
                     /locus_tag="WIB82_000676"
     CDS             7926..12101
                     /gene="rpoC"
                     /locus_tag="WIB82_000676"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ3842012.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12393..12650
                     /locus_tag="WIB82_000677"
     CDS             12393..12650
                     /locus_tag="WIB82_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842013.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            12647..12796
                     /locus_tag="WIB82_000678"
     CDS             12647..12796
                     /locus_tag="WIB82_000678"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3842014.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            12975..13346
                     /gene="rpsL"
                     /locus_tag="WIB82_000679"
     CDS             12975..13346
                     /gene="rpsL"
                     /locus_tag="WIB82_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ3842015.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13464..13934
                     /gene="rpsG"
                     /locus_tag="WIB82_000680"
     CDS             13464..13934
                     /gene="rpsG"
                     /locus_tag="WIB82_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ3842016.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13953..16058
                     /gene="fusA"
                     /locus_tag="WIB82_000681"
     CDS             13953..16058
                     /gene="fusA"
                     /locus_tag="WIB82_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ3842017.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEVDKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            16144..>16268
                     /locus_tag="WIB82_000682"
     CDS             16144..>16268
                     /locus_tag="WIB82_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ3842018.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILSKKFGG"
BASE COUNT         4340 a         3616 c         4199 g         4113 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgtcggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa agtggatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgctgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcgc attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 cccccgtgtt tccggtttta ttggagggag ggctaataga cctacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagttcgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct gctttgggtc
     1201 agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc caaggtatgg
     1261 agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg caaaaaggta
     1381 gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agatcctgat ttgactgctg ctgacttgga tgcggctgtc cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg caattgatga
     1621 agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg ttgacgtatc
     1681 tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggat cggtcgttct
     1741 gcctaaaggc accggtaaga taacccgtgt ggctgtattt actcaaggtg caaatgcaga
     1801 agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcgggcaat ctgaactttg acgttgttat tgcttctccc gatgcaatgc gtattgttgg
     1921 tcaattgggt accatcttgg gtcctcgtgg tctgatgcca aaccctaaag taggtacggt
     1981 tacccctaat gttgccgaag cagtaaaaaa tgcaaaagca ggtcaagtgc agtatcgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcattcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg ctgccgctaa
     2161 aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta ttcgcgttga
     2221 tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt ttctgggctg
     2281 cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat cgtaagattt
     2341 aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt gcttgtaaga
     2401 tgtcttttta ggttaccgcg ctggtgggat atcattttgg tatcctgttt ataaacagtg
     2461 ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag gaaattagcg
     2521 cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc agtgtttcca
     2581 gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgt gttctgaaaa
     2641 atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc gatcaaatgg
     2701 ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa gtgttgcacc
     2761 aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat ggcgaagtaa
     2821 tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag ctgttgtcca
     2881 aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg gctgctttgg
     2941 cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta atcaattatt
     3001 ttttaataca atatttggag taaaatagca tggctattac taaagaagac attttggaag
     3061 cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt gaagaaaaat
     3121 ttggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt gctgccgatg
     3181 ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggcgatcaa aaagtcggcg
     3241 tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa gacatcgttg
     3301 acggcgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa gacatccaaa
     3361 aacaactgga agaagcaggc gctaaagtcg aaatcaaata atttgatgct tcttatgaag
     3421 gctggcagtt ttctgccagc cttattttgc ttcttaaaat aaacatcaag tattgtttac
     3481 atttatttgc atagttttta tcaagtcatt gcaaataaat gtaaatatca gattgatgcg
     3541 taccgttgtt tcagacggcc tattattgaa aattactttt cggagtgtgt atgaactatt
     3601 cgtttaccga gaaaaaacgt atccgtaaga gttttgcaaa gcgggaaaat gttttggaag
     3661 ttcctttcct gctagcaact cagattgatt cttatgcgaa atttttgcaa ctggaaaatg
     3721 cttttgacaa acgtaccgat gacggtctgc aggcggcatt taattctatt ttcccgattg
     3781 tgagccataa cggttatgcg cgattggagt ttgtgcatta cacattgggc gagcctttgt
     3841 tcgatattcc cgaatgtcag ttgcgcggaa tcacttatgc agcccccttg cgcgcgcgta
     3901 tccgtttggt gattttggat aaggaagcat ctaaaccgac ggtaaaagaa gttcgtgaaa
     3961 acgaagtgta tatgggcgaa attccgttga tgaccccgag cggttctttt gtgattaacg
     4021 gcacagagcg tgtgattgtc tcccagttgc accgttcgcc cggcgtattc ttcgagcatg
     4081 acaaaggtaa gacgcactct tccggcaaat tgttattctc cgcccgcatc attccctacc
     4141 gtggttcatg gttggatttt gaatttgatc cgaaagattt gctgtatttc cgtatcgacc
     4201 gccgccgtaa aatgccggta acgattttgt tgaaggcttt gggctacaac aatgagcaaa
     4261 tcttggatat tttctacgac aaagaaacgt tctatttgtc ttcaaacggt gttcaaaccg
     4321 atttggtcgc aggccgtctg aaaggcgaaa ctgccaaggt cgatatcttg gataaagaag
     4381 gcaatgtatt ggttgccaaa ggtaagcgca ttactgcgaa aaatatccgt gatattacca
     4441 atgcaggcct gacccgtttg gatgtagaac cggaaagcct gctgggtaaa gcattggctg
     4501 ccgatctgat tgattcggaa accggcgagg tattggcttc tgccaatgat gaaattacag
     4561 aagagttgtt ggccaaattt gatatcaacg gcgtaaaaga aattacgacc ctttacatca
     4621 atgagttgga tcagggtgct tatatctcca acaccttgcg tacggatgag actgccggcc
     4681 ggcaggcggc gcgtgttgcg atttaccgta tgatgcgtcc gggcgaaccg cccaccgaag
     4741 aggcggtcga gcaattgttt aaccgcttgt tcttcagtga agacagctac gatttgtccc
     4801 gcgtaggccg tatgaaattt aatacgcgca catacgaaca aaaactgtcc gaagcccaac
     4861 aaaactcttg gtacggccgc ctgctgaacg aaacgtttgc cggtgctgcc gaaaaaggcg
     4921 gttatgtcct gagcgtcgaa gatattgtcg cctcgattgc gactttggtc gagttgcgta
     4981 acggccatgg tgaagttgac gatatcgatc acttgggcaa ccgccgagta cgttcggtag
     5041 gcgagctgac tgaaaaccaa ttccgcagcg gtttggcccg tgtggaacgt gctgtaaaag
     5101 aacgtttgaa tcaggcggaa tcagaaaact tgatgccgca cgatttgatt aatgcaaaac
     5161 ctgtttctgc cgctattaaa gaattcttcg gctccagcca attgagtcag tttatggatc
     5221 agaccaaccc cttgtctgaa gtaacccata aacgccgcgt atccgcattg ggtccgggcg
     5281 gtttgacccg cgaacgtgca ggatttgagg tgcgggacgt gcatccgacc cactacggcc
     5341 gcgtatgtcc gattgaaacg cctgaaggtc cgaacatcgg tttgatcaac tcattgtccg
     5401 tttatgcgcg caccaatgat tacggtttct tggaaacgcc ttaccgccgc gttatcgacg
     5461 gcaaagtaac cgaggaaatc gattacttgt ctgccatcga agaaggccgc tatgtgattg
     5521 cacaggcgaa tgccgatttg gattcagatg gcaatctgat tggcgatttg gttacctgtc
     5581 gtgaaaaagg cgaaaccatt atggcaacgc ccgaccgcgt ccaatatatg gacgtggcaa
     5641 ctggtcaagt ggtatccgtt gcagcatccc tgattccgtt cttggaacat gatgacgcga
     5701 accgcgcatt gatgggtgcc aacatgcaac gtcaggcagt gccttgcttg cgtcctgaaa
     5761 aaccgatggt cggtaccggt atcgagcgtt ccgttgccgt tgactctgct actgcaatcg
     5821 ttgcccgccg aggcggcgtg gtcgagtatg tcgatgccaa ccgtgttgtg attcgtgtcc
     5881 atgacgacga agcgactgcc ggcgaagtgg gtgtcgatat ttacaacttg gttaaattta
     5941 cccgttccaa tcaatctact aacatcaacc aacgtccggc tgtcaaagca ggcgatgttt
     6001 tgcaacgcgg cgatttggtg gccgatggcg cgtccaccga tttgggcgaa ttggctttgg
     6061 gtcaaaatat gaccatcgcc ttcatgccgt ggaacggtta caactacgaa gactcgattc
     6121 tgatttccga aaaagtggct gcggacgacc gctatacttc gattcacatt gaggaattga
     6181 atgtcgttgc ccgcgatacc aagctgggtg cggaagacat tacccgcgat attccgaact
     6241 tgtccgagcg tatgcaaaac cgtttggacg aatccggtat cgtttacatc ggtgcggaag
     6301 tagaagccgg cgatgtgttg gtaggcaagg taacgcctaa aggcgaaacc cagctgacgc
     6361 cggaagaaaa actgctgcgc gccatcttcg gtgaaaaagc atctgacgta aaagatactt
     6421 cattgcgtat gcctaccggc atgagcggta ccgttatcga cgttcaagtc ttcactcgtg
     6481 aaggtattca acgcgacaaa cgtgctcaat ccattatcga ttccgagttg aaacgctacc
     6541 gtttggattt gaacgaccaa ttgcgtattt tcgacaacga cgcattcgac cgtatcgagc
     6601 gtatgattgt cggtcagaaa gccaacggcg gcccgatgaa gctggccaaa ggcagtgaaa
     6661 tcacgaccga atatctggcg ggtctgccga gcaggcacga ttggttcgat atccgtctga
     6721 ccgatgaaga tttggccaag cagttggaac tgattaaagt gagcctgcaa caaaaacgcg
     6781 aagaagcgga cgagttatac gaaatcaaga agaaaaaact gacccaaggc gacgaattgc
     6841 aacccggcgt acaaaaaatg gtgaaagttt ttatcgccat caaacgccgt ctgcaagccg
     6901 gcgacaaaat ggcgggccgc cacggtaaca aaggcgtggt atcgcgcatt ctgccagtgg
     6961 aagacatgcc ttacatggcg gacggccgtc cggtagacat cgtactgaac ccattgggcg
     7021 taccttcccg tatgaacatc ggtcagattt tggaagttca cttgggttgg gcagcaaaag
     7081 gtatcggcga gcgtatcgac cgtatgctga aagagcaacg caaagcaggc gagttgcgcg
     7141 agttcttgaa caaactctac aacggcagcg gcaagaaaga agatttggat gccctgactg
     7201 atgaagaaat catcgaactg gcctccaacc tgcgtaaagg tgcatctttc gcctctcctg
     7261 tattcgacgg tgcgaaagag tctgaaatcc gcgaaatgtt gaacttggct tatccgagcg
     7321 acgatcctga ggttgaaaaa ctgggcttca acgacagtaa aacccaaatc acgctgtatg
     7381 acggccgttc aggcgaagca tttgaccgca aggttacagt cggtgtgatg cactatctga
     7441 aactgcacca cttggttgac gaaaaaatgc acgcgcgttc taccggtccg tacagtctgg
     7501 ttacccagca gcctttgggc ggtaaagccc agttcggcgg ccaacgtttc ggcgagatgg
     7561 aggtttgggc attggaagca tacggcgcgg catacacgct gcaagagatg ctgactgtga
     7621 agtctgacga cgtgaacggc cgtaccaaaa tgtacgaaaa catcgtcaaa ggcgaacaca
     7681 aaatcgatgc cggtatgccc gagtccttca acgtattggt caaagagatt cgctcactgg
     7741 gcttggatat cgatttggaa cgttactaaa caaaagtttt cagacggcct ttcagggtcg
     7801 tctgaaaaag tggtttcaga ataagaatga agcaatcggc atttaggccg tctgaaatca
     7861 aaagtaccgt ttccccatat cgaaaatccg ccatgcggta aaaatacttc cttcaaggag
     7921 caaaaatgaa tttgttgaac ttatttaatc cgttgcaaac tgccggcatg gaagaagagt
     7981 ttgatgccat taaaatcggt attgcctctc ccgaaaccat ccgctcatgg tcttatggcg
     8041 aagtcaaaaa acctgaaacc atcaactacc gtacgttcaa acctgagcgc gacggtttgt
     8101 tctgtgccaa aatctttggc ccggtcaaag actacgaatg cttgtgcgga aaatacaaac
     8161 gcttgaaatt taaaggcgta acgtgtgaaa aatgcggcgt ggaagtaacc ctgtccaaag
     8221 tgcgccgcga acgcatgggt catatcgaat tggctgcgcc cgtcgcacat atttggttct
     8281 taaaatccct gccttcccgc ttgggtatgg tgttagacat gactttgcgc gacatcgagc
     8341 gcgtattgta ctttgaagca tttgtggtaa ccgatcccgg tatgactccg ctgcaacgcc
     8401 gccaattgct gactgaagac gattactaca acaagctgga cgaatacggc gacgacttcg
     8461 atgccaaaat gggtgcagaa ggtatccgcg aattgttgcg taccctgaat gtagcgggcg
     8521 aaatcgaaat cctgcgccaa gagttggaat cgactggttc cgacaccaaa atcaaaaaaa
     8581 tcgccaaacg cttgaaagta ttggaagcct tccatcgttc cggtatgaaa ctggaatgga
     8641 tgattatgga tgtgctgccg gtattgccgc ctgatttgcg tccgttggtt ccattggatg
     8701 gtggtcgttt tgccacttcc gatttgaacg atttgtaccg ccgcgttatt aaccgtaaca
     8761 accgtctgaa acgtctgttg gaactgcacg cgcctgacat catcgtccgc aacgaaaaac
     8821 gtatgttgca agaagcggtc gactcgctgt tggataacgg ccgtcgcggt aaagccatga
     8881 ccggcgccaa caaacgtccg ctgaaatcat tggctgacat gattaaaggt aaaggcggcc
     8941 gcttccgtca aaacctgctg ggtaaacgtg tggactactc tggtcgttcc gtgattaccg
     9001 taggtccata cctgcgcctg caccaatgtg gtctgccgaa aaaaatggct ttggaactgt
     9061 tcaaaccgtt cattttccac aaattggaaa aacaaggtct ggcttccacc gttaaagcag
     9121 cgaaaaaatt ggtagaacaa gaagtgccgg aagtatggga catcttggaa gaagtcattc
     9181 gcgaacatcc gattatgctg aaccgtgcgc caaccctgca ccgtttgggt attcaagcgt
     9241 tcgaacctat cctgattgaa ggtaaagcga ttcagttgca cccattggtg tgtgccgcat
     9301 ttaacgccga ctttgacggt gaccaaatgg ctgtacacgt cccattgagc ttggaagcac
     9361 aaatggaggc acgcacgctg atgctggctt caaacaacgt attgtctccg gccaacggcg
     9421 aaccgattat cgtaccttcc caagacatcg tattgggcct gtactacatg acccgcgacc
     9481 gtatcaatgc caaaggcgag ggcagcctgt ttgccgatgt gaaagaagtg catcgcgcat
     9541 accataccaa acaggttgaa ctgggtacga aaattaccgt acgtctgcgc gaatgggtga
     9601 aaaacgaagc aggtgagttt gagcctgtcg ttaaccgtta cgaaacaacc gtcggccgtg
     9661 cattgttgag cgaaatcctg ccgaaaggcc tgccgtttga atatgtcaac aaagcgttga
     9721 agaaaaaaga aatttctaaa ctgattaacg catcgttccg cctgtgcggc ttgcgcgata
     9781 cggttatctt tgctgaccac ctgatgtaca ccggtttcgg atttgcggca aaaggcggta
     9841 tttccattgc cgttgacgat atggaaattc caaaagaaaa agcggccttg ctggctgaag
     9901 ccaatgccga ggttaaagaa atcgaagacc aataccgtca aggtttggtt accaacggcg
     9961 aacgctacaa caaggtggtc gatatttggg gtcgtgccgg cgataagatt gctaaagcga
    10021 tgatggacaa cttgtccaaa caaaaagtta tcgaccgtga cggcaacgaa gtcgatcaag
    10081 agtcattcaa ctccatttat atgatggcgg actccggtgc ccgtggttct gcagctcaga
    10141 ttaaacagtt gtccggtatg cgtggcttga tggctaaacc tgacggctcg attattgaaa
    10201 cgccgattac ctcaaacttc cgtgaaggtc tgaccgtatt gcaatacttt attgcgaccc
    10261 acggtgcgcg taagggtttg gcggataccg cattgaaaac cgcgaactcc ggttacctga
    10321 ctcgtcgtct ggtagacgta actcaagatt tggtcgttgt tgaagacgat tgcggtactt
    10381 cagacggctt tgtcatgaag gcagtggtac aaggcggtga tgtgattgaa gcattgcgcg
    10441 atcgtatttt gggtcgtgtt accgcgtctg acgttgtcga tccgtcaagt ggcgaaacct
    10501 tggttgaagc cggtacgttg ctgactgaaa aactggtgga tatgatcgac caatccggtg
    10561 tcgatgaagt caaagtccgt acgccgatta cttgtaaaac ccgtcacggc ctgtgtgcac
    10621 actgttacgg tcgtgacttg gcacgcggca aactggttaa cgccggtgag gcagtcggtg
    10681 tgattgctgc acaatccatt ggcgaaccgg gtacccagtt gaccatgcgt acgttccaca
    10741 tcggtggtgc ggcatcccgt gcggcagcag ccagccaagt ggaagccaaa tccaacggta
    10801 cggcacgatt cagcagccag atgcgctacg ttgccaacaa caaaggcgag ttggttgtca
    10861 tcggccgttc ttgtgaagtc gtgattcacg acgatatcgg ccgtgaacgc gaacgccaca
    10921 aagtacctta cggtgccatc ctgctggtac aagacggtat ggccattaaa gccggtcaaa
    10981 ccttggcaac ctgggatccg catacccgtc cgatgattac cgaacacgca ggtatggtga
    11041 aattcgaaaa cgtggaagag ggcgttaccg ttgccaaaca aaccgatgat gtaaccggtt
    11101 tgtccacttt ggtggtgatt gacggtaaac gtcgttcctc tagtgcttcc aaactgctgc
    11161 gtccgactgt gaaactcttg gacgaaaacg gcgtggaaat ctgtattccc ggtacttcta
    11221 ctccggtatc catggcattc cccgttggtg cggtgattac cgtacgcgaa ggtcaggaaa
    11281 tcggtaaagg cgacgtattg gcgcgtattc cgcaagcctc ttccaaaacc cgcgacatta
    11341 ccggcggcct gccgcgcgtt gccgagctgt ttgaagcacg cgtgccgaaa gatgccggta
    11401 tgttggcgga aattaccggt accgtttcct tcggcaaaga gaccaaaggg aagcaacgtc
    11461 tgattatcac tgacgtggac ggtgtagcat acgaaaccct gatttctaaa gagaaacaga
    11521 ttctggtaca cgacggtcaa gtggtaaacc gcggcgaaac cattgtggac ggcgcggtcg
    11581 atccgcacga tattctgcgt ctgcaaggta tcgaagcact ggcacgctac attgtccaag
    11641 aggtgcaaga ggtttaccgt ctgcaaggtg tgaagatttc tgataaacac atcgaagtca
    11701 tcatccgtca aatgttgcgc cgtgtgaaca ttgcggatgc cggcgaaacc gggttcatta
    11761 ccggagagca ggtcgaacgc ggcgatgtga tggcggccaa tgaaaaagct ttggaagaag
    11821 gcaaagagcc ggcacgttac gaaaacgtat tgctgggtat taccaaagcc tccctgtcca
    11881 ccgacagttt catttctgcc gcatcgttcc aagaaacgac ccgtgttctg accgaagccg
    11941 cgattatggg caaacaagac gaactgcgcg gtttaaaaga gaacgtcatc gtcggccgcc
    12001 tgattcctgc cggtaccggt ttgacttatc accgcagccg tcatcagcaa tggcaagggg
    12061 tagagcagga gactgctgaa acccaagtaa cggatgaata atcttcggcg cgtttattca
    12121 atacaaaacc gcaagcttta agcttgcggt ttttctttgt ccgattaagg caaaaacaag
    12181 cgttttcgtc attttgaggc gtgtggatta ttccttaggt attttcgggc cggagaccaa
    12241 cgaggtggcg ggtgtcgtcg gtacgtccgg agaccaaaat aactttgcca gggatgttgg
    12301 tttcggcggt caaaaaaagt agcgtcttaa tgttttccat ttaaacaaat gtcgtctgaa
    12361 acttcagacg gcatttcctt taaaaaataa atatgaaacc cagaaatctc ttttttgcag
    12421 gctgcctgct gacttcggcg acgtttgccg aggatatcgg cgtacctgtc gaactgatta
    12481 acgtcggtaa tcggattgcg atgccgtctg aaggggaaag cctcgccctc ctgccgtttg
    12541 ccgaggatgt accgccggtt cgcgatgcaa tgccgtctga agttcctaaa agcgcggcag
    12601 gcggcgatgt tcggggtgac cggatgggaa tgccgattaa catcggatga gcgcggcttt
    12661 atggcataaa aaactgtcgt ggaaaggatt tacaccccaa ataaatttcc gttacaacaa
    12721 gatcaacagc aatatgcccg ccttttattc gcgcagcggc aaggaacggt ttgtcagtat
    12781 agaaaaaacg tattgacagt attttcttca gtcgtccgac tgattgtgag ggatgtcggt
    12841 aaatatttat cggcaaacaa gaaaatcatc tttcttcttg tcgttatgct tgactgtata
    12901 cttgcaataa aaatataatt ccactcttgc cgacatggtg tcggcaagta tttaactcaa
    12961 caggacgaga aaatatgcca actatcaacc aattggtacg caaaggccgt caaaagcccg
    13021 tgtacgtaaa caaagtgccc gcactggaag cctgcccgca aaaacgcggc gtgtgcaccc
    13081 gtgtatacac gactacccct aaaaaaccta actctgcatt gcgtaaagta tgtaaagtcc
    13141 gcctgaccaa cggttttgaa gtcatttcat acatcggcgg cgaaggccac aacctgcaag
    13201 agcacagtgt cgtattgatt cgcggcggtc gtgtaaaaga cttgccaggt gtgcgttacc
    13261 acactgtacg cggttctttg gatactgcag gcgttaaaga ccgtaaacaa gcccgttcca
    13321 aatacggtgc taagcgtcct aaataattac tgggacttaa ataggcacgt cggccgccta
    13381 agctgaacaa cggccgagta agtgaatact caattgggta ttcatgggaa tagacccgac
    13441 tgaatagatt aaaggaaatt aaaatgccaa gacgtagaga agtccccaag cgcgacgtac
    13501 tgccagatcc taaattcggc agcgtcgagt tgaccaaatt catgaacgta ttgatgattg
    13561 acggtaaaaa atccgttgcc gagcgtatcg tttacggtgc gttggaacag attgagaaaa
    13621 aaaccggcaa agtagcaatc gaagtattta acgaagccat tgcaaacgcc aaacctatcg
    13681 tggaagtgaa aagccgccgt gtaggtggtg caaactacca agttcctgtt gaagttcgtc
    13741 cttcacgccg tttggctttg gcaatgcgct gggttcgcga tgcggcccgc aaacgtggtg
    13801 agaaatccat ggacctgcgt ttggcaggcg aattgattga tgcgtccgaa ggccgtggcg
    13861 gtgcgttgaa aaaacgtgaa gaagtacacc gtatggctga agccaacaaa gcattctctc
    13921 acttccgttt ctaattttga aaggctaata aaatggctcg taagaccccg atcagcctgt
    13981 accgtaacat cggtatttcc gcccatatcg atgcgggtaa aaccacgacg acagaacgta
    14041 ttttgttcta taccggtttg acccacaagc tgggcgaagt gcatgacggt gcggctacta
    14101 ccgactacat ggaacaagag caagagcgcg gtattaccat tacctccgct gccgttactt
    14161 cctactggtc cggtatggcg aaacaattcc ccgagcaccg cttcaacatc atcgacaccc
    14221 cgggacacgt tgactttacc gtagaggtag agcgttctat gcgtgtattg gacggcgcgg
    14281 taatggttta ctgcgcggtg ggcggtgttc aaccccaatc tgaaaccgta tggcggcaag
    14341 ccaacaaata ccaagtgccg cgcttggcgt ttgtcaataa aatggaccgt cagggtgcca
    14401 acttcttccg tgttgtcgag caaatgaaaa cccgtttgcg cgccaatcct gtacctatcg
    14461 tcattccggt tggtgcggaa gacaacttca gcggtgtggt tgatttgttg aaaatgaaat
    14521 ccatcatttg gaatgaagtc gataaaggta caacctttac ctatggcgat attcctgccg
    14581 aattggtcga aactgccgaa gaatggcgtc aaaatatgat tgaagccgca gccgaagcca
    14641 gcgaagaact gatggacaaa tacttaggcg gcgacgagct gaccgaagaa gaaatcgtag
    14701 gcgcgttgcg tcaacgtact ttggcaggcg aaattcagcc tatgctgtgt ggttctgcat
    14761 ttaaaaacaa aggtgttcaa cgtatgttgg acgcagttgt agaattgctg ccagctccta
    14821 ccgatattcc tccggttcaa ggtgtcaacc cgaataccga ggaagccgac agccgtcaag
    14881 ccagcgatga agagaaattc tctgcattgg cgttcaaaat gttgaacgac aaatacgtcg
    14941 gtcagctgac ctttatccgc gtttactcag gcgtagtaaa atccggcgat accgtattga
    15001 actccgtaaa aggcactcgc gaacgtatcg gtcgtttggt acaaatgact gccgcagacc
    15061 gtactgaaat cgaagaagta cgcgccggcg acatcgcagc cgctattggt ctgaaagacg
    15121 ttactaccgg tgaaaccttg tgtgcggaaa gcgcgccgat tatcttggaa cgtatggaat
    15181 tccccgagcc ggtaatccat attgccgttg agccgaaaac caaagccgac caagagaaaa
    15241 tgggtatcgc cctgaaccgc ttggctaaag aagacccttc tttccgtgtc cgtacagacg
    15301 aagaatccgg tcaaaccatt atttccggta tgggtgagct gcacttggaa attattgttg
    15361 accgtatgaa acgcgaattc ggtgtggaag caaatatcgg tgcgcctcaa gtggcttacc
    15421 gtgaaactat ccgcaaagcc gttaaagccg aatacaaaca tgcaaaacaa tccggtggta
    15481 aaggtcaata cggtcacgtt gtgattgaaa tggaacctat ggaaccgggt ggtgaaggtt
    15541 acgagtttat cgatgaaatt aaaggtggtg tgattcctcg cgaatttatt ccgtctgtcg
    15601 ataaaggtat ccgcgatacg ttgcctaacg gtatcgttgc cggctatcct gtagttgacg
    15661 tacgtatccg tctggtattc ggttcttacc atgatgtcga ctcttcccaa ttggcatttg
    15721 aattggctgc ttctcaagcg tttaaagaag gtatgcgtca agcatctcct gccctgcttg
    15781 agccaatcat ggcagttgaa gtggaaaccc cggaagaata catgggcgac gtaatgggcg
    15841 acttgaaccg ccgtcgcggt gttgtattgg gtatggatga tgacggtatc ggcggtaaaa
    15901 aagtccgtgc cgaagtacct ttggcagaaa tgttcggtta ctcgaccgac ctgcgttctg
    15961 caacccaagg ccgcgctact tactctatgg agttcaagaa atattctgaa gctcctgccc
    16021 acatagctgc tgctgtaact gaagcccgta aaggctaatc agaaaaggcc gtctgaaact
    16081 gaaaataaat tttcagacgg ccattgttct ttaatcgatc tttatatgta aaggaattag
    16141 ctcatggcta aggaaaaatt tgaacgtagc aaaccgcacg taaacgttgg caccatcggt
    16201 cacgttgacc atggtaaaac cactctgact gctgctttga ctactatttt gtctaaaaaa
    16261 ttcggtgg
//
LOCUS       DAWHRS010000030        16034 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWHRS010000030 DAWHRS010000000
VERSION     DAWHRS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16034
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.030"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(118..1362)
                     /gene="clpX"
                     /locus_tag="WIB82_000683"
     CDS             complement(118..1362)
                     /gene="clpX"
                     /locus_tag="WIB82_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ3842019.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            1553..1924
                     /gene="rbfA"
                     /locus_tag="WIB82_000684"
     CDS             1553..1924
                     /gene="rbfA"
                     /locus_tag="WIB82_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ3842020.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            1957..2880
                     /gene="truB"
                     /locus_tag="WIB82_000685"
     CDS             1957..2880
                     /gene="truB"
                     /locus_tag="WIB82_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ3842021.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            3097..5199
                     /locus_tag="WIB82_000686"
     CDS             3097..5199
                     /locus_tag="WIB82_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEZ3842022.1"
                     /translation="MKTDIQTELTQVLLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLSNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDSNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPASQPASQPASQPASQPASQP
                     ASQPASQLYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQAVGEIKR
                     HSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYNTGNDGFKYNDKFNHSTW
                     LTFMKNRLEIAKELLMKDGSIFVSIDDNEQAYLKILMDEVFGNENFICNFIWEKKTGA
                     SDAKQIATITEFVLCYSKNFKTVKLNKNTFSYDTERYKLSDKFEQERGKYYIDNLDRG
                     GLQYSDSLNFAIQCPDGTFTYPNGRTEFVNDGWIWKWSKNKIDWAITNGFLEFRKSKS
                     KKSGWSVCYKNYMLVDNENKPIERSAPYKNLIQDILNTHATDELKKLFGSKVFTTPKP
                     ESLLQYLIQIATSESDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKK
                     VIDGEQGGISKAVNWQGGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYF
                     LKYNVSINEFSQTIQEPEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFAGCLND
                     DDKALSRAFYQTEKKDGE"
     gene            5189..7969
                     /locus_tag="WIB82_000687"
     CDS             5189..7969
                     /locus_tag="WIB82_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ3842023.1"
                     /translation="MANNKMLFEVIENRKAVYLEDGDDEKCRLPEFVERNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIGSTHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQGELDLEKEIND
                     RTSNAEIERKGWEHMVLELLLNKNGNPSENVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRPDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKTLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(8113..9285)
                     /locus_tag="WIB82_000688"
     CDS             complement(8113..9285)
                     /locus_tag="WIB82_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ3842024.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            9576..10022
                     /gene="iscR"
                     /locus_tag="WIB82_000689"
     CDS             9576..10022
                     /gene="iscR"
                     /locus_tag="WIB82_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ3842025.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            10051..11265
                     /locus_tag="WIB82_000690"
     CDS             10051..11265
                     /locus_tag="WIB82_000690"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ3842026.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            11526..11912
                     /gene="iscU"
                     /locus_tag="WIB82_000691"
     CDS             11526..11912
                     /gene="iscU"
                     /locus_tag="WIB82_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ3842027.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            11999..12319
                     /gene="iscA"
                     /locus_tag="WIB82_000692"
     CDS             11999..12319
                     /gene="iscA"
                     /locus_tag="WIB82_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ3842028.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(12279..12500)
                     /locus_tag="WIB82_000693"
     CDS             complement(12279..12500)
                     /locus_tag="WIB82_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ3842029.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            12582..13082
                     /gene="hscB"
                     /locus_tag="WIB82_000694"
     CDS             12582..13082
                     /gene="hscB"
                     /locus_tag="WIB82_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ3842030.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKKSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            13180..15930
                     /gene="gyrA"
                     /locus_tag="WIB82_000695"
     CDS             13180..15930
                     /gene="gyrA"
                     /locus_tag="WIB82_000695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ3842031.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGLVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
BASE COUNT         4682 a         4126 c         3599 g         3627 t
ORIGIN      
        1 tcagacaatc cgtaaaactc aaatgccgtc tgaaggcggg aaggcttcag acggcatcag
       61 aaacaaaaaa ccgcttcaga aacgtggttc aacgttccga agcggttttg tttgccatca
      121 ggactcgaac accaattccg gttccctgcc ctcttcgatg actgccttac cgaccaccac
      181 ttttttcaag cctttcaaat caggcaggcg gtacatcgta tcgagcaggc agcgttcgac
      241 gatggacctc aggccgcgcg cgccggtttt gcgttccatt gcctgacgcg cgatggaacg
      301 caatgcgcct tcttcaaact ccagttcgac attttccata ccgaacaacg cctgatactg
      361 cttgaccaaa gcatttttcg gctcggtcaa aatattaatc agcgcgtctt cgtccaattc
      421 ttctaaagtt gcaatcacag gcaaacgtcc gattaattct ggaatcagac cgaatttaat
      481 caagtcttcc ggttcgacga tgccgaacag cttggtaatg tcggcatttt cgtccttgct
      541 gtgaacggac gcaccgaaac cgataccgcc tttttcagta cgctggcgga tgactttttc
      601 caagcctgca aacgcgccgc cgcagataaa caggatgttg gtggtatcga cgttgataaa
      661 ttcctgattc ggatgcttgc ggccgccttg gggcggaacg ctggccactg taccttcaat
      721 cagtttcaac aaggcttgtt gcacaccttc gccggatacg tcgcgggtaa tcgacgggtt
      781 gtcgcttttg cgtgaaattt tatcgatttc gtcgatatag acaatgccgc gctgggcttt
      841 ttcgacatcg aaatcacatt tgcccaaaag cttggtaatg atttgctcga cgtcttcgcc
      901 gacataacct gcttcagtca aagttgtggc atccgccatc acgaacggca catccagttt
      961 gcgcgccaaa gattgcgcca gcagggtttt acccgatccg gtcgggccga taagcaggat
     1021 gttggatttc gacaattcga cattagctcc tgctttagga tggcgcaggc gtttgtaatg
     1081 gttgtacacc gacaccgcca aggctttctt ggcttgttcc tgaccgataa cgtggtcgtt
     1141 gaggttggcg acgatttcgg cgggcgtggg cagcttgccg gattcttccg gctcccctcc
     1201 ggcactttcc gaaggcgtgc cgtcatttcc gtcttcatgc aatatttcaa tacagtttga
     1261 gacgcattcg tcacagataa aggcgttttc gccctcaatt aaatgtttga cgtgtgattt
     1321 ggattttccg caaaaggaac aagtacggtt ttcgttggac atggctttct ttacaatgta
     1381 tgcgttacag aaaacggcac gtgccgttcg ggttgccaag tataataact atatccgttc
     1441 ttatcaatgt attaccttaa aatcccgccg attaggctat aatacgccct ttcgcaaccg
     1501 ccccggcggc aaaaatgccg tctgaaacca aatctgaaat ctgaggatat tcatgagaaa
     1561 accccaacgc ggctatgccc gccaagaccg tgtcaaagaa caaattatgc gcgagcttgc
     1621 cgaactcgtc cgtaccggac tgaaagaccc gcgcgccggc ttcattaccg tcaacgaagt
     1681 cgaagttacc cgcgattaca gccacgccac cgtgttctac accattttaa accaagacgc
     1741 gcgcgaaatt acggaagaag tgctggaaca cgcgcgcgga cacctccgca gcgaattggc
     1801 caaacgcatc aagctgttca aaacgcccga actgcatttc aaatacgacg aatctttgga
     1861 acgcggtttg aacctgtccg cccttatcga ccaagtagcg gcggaaaaac cggttgaaga
     1921 ctgacggata tgcccatgcc gtccgaacat cgaaccatga atacaggcaa accccaaaaa
     1981 cgtgccgtca acggtgtttt gctcttggac aaacccgaag gcctttccag caacaccgcg
     2041 ctgcaaaaag cgcggcgttt gtttcatgcc gaaaaagccg gacataccgg cgtgctcgac
     2101 cctttggcaa ccggactttt gcccgtctgc ttcggtgaag cgaccaagtt cgcccaatac
     2161 ctgctggatg ccgacaaagc ctacaccgcc acgctgaaac tcggcgaagc cagcagcacg
     2221 ggtgatgccg aaggcgaaat cattgccacc gcccgcgccg atatttcctt agccgaattt
     2281 cagacggcct gccaagcact gacaggcaac atccgccaag tgccgccaat gttttccgcg
     2341 ctcaagcacg aaggcaaacc gctgtacgaa tacgcccgca aaggcatcgt catcgaacgc
     2401 aaagcgcgct acattaccgt ttacgccatc gatattgccg aatttgacgc gcccaaagcc
     2461 gtcatcgacg tacgttgcag caaaggcacc tacatccgca ccctcagcga agacatcgcc
     2521 aaacacatcg gcacgttcgc ccacctgacc gccctgcgcc gcactgaaac cgccggcttt
     2581 accatcgccc aaagccacac gcttgaggcc ttggcaaatt tgaacgaaac agaacgcgac
     2641 agcttgctgc taccctgcga cgtattggtt tcacactttc cccaaaccgt tttaaacgat
     2701 tatgccgtcc atatgctcca ctgcggacaa cgtccgcgtt tcgaagaaga cctgccttcc
     2761 gacacgccgg tacgcgttta cacggaaaac ggccgctttg tcggtctggc ggaatatcaa
     2821 aaagaaatat gccgtctgaa agccttgcgc ctgatgaaca cggcggcatc cgccgcctga
     2881 acggcggtta aaaatacagg ctgtgcttga ataatgtgtt gatatttccg caaaatcccg
     2941 acacactcgg acacccgccc cgcttatcgc aactttgcga acgcccccgg aaacagcaaa
     3001 gacatcaaat aattgatttt attagaatct atttgcaaag ccatttgccg ttacacaaga
     3061 atggcacatt aaaataactg atgaggattt ataacgatga agacagacat tcaaaccgaa
     3121 ttaacccaag tcctactacc acacgaaaaa gtatgggcga acgaagaaaa aaccatttta
     3181 gccaaaaaca tcctgttgga tttggtggaa aaaaccgacc cgaccattat cggtttgtta
     3241 ttgagtaatg atgagttaaa acgccatttc tttgtggaag tgaatggtgt gctggtgttt
     3301 aaattgcagg atttccgttt tttcttggac aaacacagcg tcaataattc ctacacaaaa
     3361 tacgccaacc gcattggttt gacggacagc aaccgctttt tgaaagacag cagtgatatt
     3421 gtgttggatt ttccgtttaa agattgtgtg ttaaatggcg gacaaagcac cgaggaaggc
     3481 gaagaaattt attttaaacg caataacagc cagccagcca gccagccagc cagccagcca
     3541 gccagccagc cagccagcca gccagccagc cagccagcca gccagccagc cagccaatta
     3601 tacactaaat taacccgaaa aagacaagaa atctttttta atcaaaccct tgcttttgat
     3661 gaaattgatc ggctttttga cgcaaaagca ttctcaaaat tctctcgcta taccgcagac
     3721 ggcaaacaag ccgttggcga aatcaaacga cattcagacg gcacacccgc cgaaaatctc
     3781 attatcaaag gcaataatct gattgccctg cattcgcttg ccaagcagtt taaaggcaaa
     3841 gtgaagctga tttatattga cccgccatat aacacgggta atgacggttt taaatacaac
     3901 gacaaattta atcattccac ttggctgact tttatgaaaa accgtctaga aatcgccaaa
     3961 gagctgctta tgaaagacgg ttcgattttt gtgtcaattg acgacaacga acaggcatat
     4021 ttgaaaattt taatggatga agttttcgga aatgaaaatt tcatctgcaa ttttatttgg
     4081 gaaaaaaaga caggtgcgtc cgatgccaaa cagatagcga ctattacaga gtttgtctta
     4141 tgttactcaa agaactttaa aacagttaaa ttaaataaaa acacgttttc ttatgataca
     4201 gagagataca aattaagtga taagtttgaa caggaaagag gcaaatatta tatcgacaat
     4261 ttagatagag ggggattgca gtatagtgac agtttgaatt ttgcaatcca atgtccagat
     4321 ggcactttta cgtatccgaa tggcaggact gaatttgtca atgatggctg gatatggaaa
     4381 tggagtaaaa ataaaattga ttgggcaata acaaacggtt ttttggagtt tagaaaatca
     4441 aagtctaaaa aatctggatg gagtgtatgt tataaaaact atatgttggt tgataacgaa
     4501 aacaagccga tagaacgttc tgctccctat aagaacttaa tacaggatat cttaaataca
     4561 catgcgacag atgaattgaa aaaactgttc ggcagcaaag tttttactac tccaaaacct
     4621 gagagcttat tgcagtatct tattcaaatt gccacatccg aatccgacat cgtcttagac
     4681 taccatcttg gtagtggcac aaccgccgcc gttgcccaca aaatgaaccg ccaatatatc
     4741 ggtattgaac aaatggatta tattgaaacg cttgctgttg aacgcttgaa aaaagtgatt
     4801 gatggcgagc aaggcggtat ttccaaagcc gtgaattggc aaggtggtgg cgaatttgtt
     4861 tatgccgaac ttgctccatt taacgaaacc gcaaaacaac agattttggc ttgcgaagat
     4921 tcagacggca tcaaaacgct gtttgaaggt ttatgcgaac gctatttctt gaaatacaac
     4981 gtcagcataa atgaatttag tcaaaccatt caagagcctg aatttcaatc tttgccatta
     5041 gacgaacaaa aacaaatggt gcttgaaatg ttggatttaa atcaaatgta tgtttcatta
     5101 tccgaaatgg acgatgaaca atttgcaggc tgtctgaatg atgacgataa agcgttaagc
     5161 cgtgcgttct atcaaacgga gaaaaaagat ggcgaataat aaaatgttgt ttgaagtgat
     5221 tgaaaaccgc aaagctgtgt atttggaaga cggcgatgat gaaaaatgca ggctgcctga
     5281 atttgtagaa cgcaatttaa aatatccgtt ttttgaatgg caaaaatcgg ctttagaaaa
     5341 ctttgtgatt tttgaccgca cttcaaaatt aaaggatttt cccgatatca aaaaccgccc
     5401 tacccatttg ctgttcaata tggcaacagg tgcaggcaaa acgatgatga tggcggcgtt
     5461 gattttgtat tattttgaaa agggttatcg gcattttctg tttttcgtca atcaaaacaa
     5521 tatcgtagat aaaacggaaa ataattttat tggctcaacg cacgcaaaat ttttatttac
     5581 cgagaagatt ttgcagggcg atacggtaat tcccattcgc aaagtggaaa tgttcagccc
     5641 acattcagac ggcattgaaa ttaaatttac cagcattcaa aagctgtata acgatatcca
     5701 caccgaacgg gaaaaccaaa ccacattggc ggatttgcac aaattgaacc ttgtgatgct
     5761 gggcgatgaa gcacaccatt taaacgcaca aaccaaaggc aaaaaacaag gcgaattaga
     5821 tttagaaaag gaaattaacg accgcactag caatgccgag attgaacgca aaggctggga
     5881 gcatatggtt ttggaattgt tactcaataa aaatggcaat cctagcgaaa atgtgctgtt
     5941 ggaatttacc gccacgctac ctgaaaatgc tgacgtgcaa caaaaatacg cggataaaat
     6001 catcacaaaa tttggcttaa aagaattttt gcaaaaaggt tataccaaag aaatcaattt
     6061 ggtatccagt acgctgggca agaaagagcg ggtgttgcac gctttattat ttgcttggta
     6121 tcgccatcaa atcgcgttga aatacggcat tgccaatttc aagcctgtga tgttgtttag
     6181 aagtaagacg attgatgaat caaaagcgga ttattcggca tttttaaatt gggcagaaaa
     6241 tgtacaagcg gatgattttg agtttttaga tacgttttca ggcagcctga ataatagcga
     6301 caacgccaac gaacaaggca aaacccgaac cgaacaagcc ctaaaattta tgcaggaaaa
     6361 tggctttgag tttgcacatt tggcaaattg ggtgaaacaa aattatcaaa aacataatgt
     6421 gattattacc aactccgaaa ccaacaaaaa caaaactgaa aaaaccgaca gcgaaaccga
     6481 aaaattgcta aacaatttgg aagcggctga taatccaatc cgtgccattt ttacggtaga
     6541 tagattaacc gaaggttggg acgttctgaa tttgtttgat attgtgcgtt tgtatgaagg
     6601 gcaaaatggc ggtggttcaa ataaaaaatc aggcaaaacg gctgccgcta ccgtatcgga
     6661 aaagcagttg attggtcgtg gcgtgcgtta ttttccattt gcgtttgaag gtaaacagcc
     6721 gaataaacgc aaatttgaca acgatatgca acacgaattg cgtatcttgg aagaattgtt
     6781 ttattacacg cacgatgagc aatcccgcta tatttcagaa ctgaaaaacg agttgcgaaa
     6841 agacggttat ttgcctgaaa aagacgatga taaggtattg gcaacattta aactcaaatc
     6901 tgaatttgcc gctaataagg attttagaga attgttaatt tgggcgaata aaaaaatccc
     6961 caacccaaac gccaaaacca ataatgcaaa cagcctgcaa gccaatccgc aaacgctttc
     7021 attccaagtt cacggcaatc aactgttgca ggaaacgcaa tttacagccg atgaaaatga
     7081 tgaaaaagtc aagcaacttg gcacgcaaaa taattttact caaaccataa aaatgagtga
     7141 aatggagcgg cacattttca ataaagccct gcatatcaaa gggaaaaata gtcaatcttt
     7201 attccatttc aaccgtttgc aaagcaagct caacattcaa aatcgcaatg aattgcaaaa
     7261 taacttgttg aaagactggc aaattgaatt tttaggatta gggcaagaca aacaggttcg
     7321 cccggacgat aaacttgcag gctgcctgaa aatcttggaa atggttgaaa aacatctgaa
     7381 tgaaagtgat atgccgttta tcggtacaaa agaatttacg cctaaaacat tatgggaaat
     7441 tttcggcaca ccaaaacaaa aatgggtcaa aaaagatgat gtaaaaacga ctattgccac
     7501 gcaaaatgat tggtatgtga tgaataattt tgccggaacg ggtttggaag aagcgttaat
     7561 tcaatttatt tcagcgcgtt tgggcgattt gaaatctcaa tatgacattc atttaatccg
     7621 taatgaagaa gtgtttaaat tgaataactt tgccgatggt gaaggattta tgccggactt
     7681 tattttattg ctgaaagata aacaaaaatc ttcttccaat ggcgtgaatg actttttgca
     7741 ttaccaaatt ttcattgagc ccaaaggtga gcatttagtg gaaactgatg gatggaaaga
     7801 agaattttta gaagccatca ctgtggaata tgggatggat aagatcctgc aaaaagatac
     7861 accgcattat cgtttgatcg gtttaccgtt ttttactgat catcagaaaa atggacaatt
     7921 tacggagtca ttccctttag gtgcggcatc gcttgaaaaa atgaagtagt gcattgcagg
     7981 cagccccgtt tgacaaaact tcctttacaa aagggcgttt tgtcagatat ttaatcaaca
     8041 cattattaaa atacagccaa attttaatgc cgtctgaacc ctgtgttcag acggcatcgt
     8101 atttttcagt atctaaaccg tttccctacc ccaatcctta cccctcaaaa tcgaggcatc
     8161 gacatcttga atatcgcggt gtcccgtaaa cgccatagat atatccattt ctttatatag
     8221 gatttccagc gcacgggtta cgccttcttc gccatacgcg cccaagccat acaggaacgc
     8281 ccgacctatc attgtacctt tcgcgcccaa agcccaagcc ttcaaaatat cctgaccgct
     8341 gcggatgccg ctgtccatcc aaacttcgat gtcgctgccc actgcgctga cgatgtcggg
     8401 caaggctttg atggcagaca cggtatcgtc gagctgtcgg ccgccgtggt tggaaacaat
     8461 caatgcgtcc gcgccgcttt tcgctgcttt ttccgcgtct tcgggttcca taatgccttt
     8521 gataatcagc ttgccgcccc acaaatcttt aatgcgcgcc acatcgtccc agctcaggcg
     8581 cgggtcgaat tgttcggaag tccatgaaga cagcgaagac aaatcgccga cgttcttcgc
     8641 gtgtccgacg atattgcgga acgtgcggcg ttccgtgttc agcattttca tacaccattc
     8701 gggcttggtc gccagattga ttaaattggc gatggtcggt ttcggcggcg cggacaggcc
     8761 gtttttgatg tctttgtggc gttgccccaa aacctgcaaa tcagcggtca ataccaatgc
     8821 cgaacatttg gcatccttcg cgcgcttaat caggttttcc ataaactcgc ggtcgcgcat
     8881 cacataaagc tgaaaccaaa atggtgcgga agtgttctcg gcaacgtctt caatcgagca
     8941 gatagacatc gtggacagcg taaacggaat gccgaacttc tccgccgccc gcgccgccaa
     9001 aatttcaccg tcggcgtgtg ccatacccgt gaaacccgtc ggcgcaatcg ccaccggcat
     9061 tttcacatcc tgcccgatca ttttggtttc caggcttcgg ccttccatat tgaccaatac
     9121 cttttgtcgg aagcggatgt ctttgaaatc cgaagtgttt tcacggtagg tagtttctgt
     9181 ccacgaaccc gaatcgatgt aatcgtaaaa catacgcggc attttgcgct tggcaacgcg
     9241 gcgcaagtct tcgatgcagg tcattttgct caaatcacgt ttcatttgtc gccccctgaa
     9301 tacctgaata actttatatg aaatcgataa tgtatatcaa tattgattat aaggcaaatc
     9361 atttcaacat ttgccgcatc cgccgcagct ccctacttta agcgacataa ggtttaaaat
     9421 tcaaaaataa caaattaaaa tcaaaatatt aaaaatcaat caatctatcg atttaaacag
     9481 ccaatcacac aatccgccct catacttgac tgaaacactc agatattgga caattccacc
     9541 cactaataaa aaaaccgaca tgggcaacca ccaccatgag actgaccacc aaagggcgtt
     9601 tcgccgttac cgctatgctg gatttggcga tgaacgcgca aaccggcgcc gtcaaactca
     9661 gtgccatcag cgaacgccaa aacatatccc tctcctatct cgagcaattg ttcggcaaac
     9721 tccgccgcgc cggacttgtt gaaagcctgc gcgggcccgg cggcggctac atcctcgccg
     9781 caccggcggc acgcatcaac atcgcccaaa tcatcgccgc cgccgaagac cggctggacg
     9841 caacccaatg cggcagcaaa gccaactgcc accacggcgc gccctgcctg acgcacgatc
     9901 tttgggagaa tttaaacaaa accatcaacg actacctcgg cagcgttacc ctgcaaagca
     9961 tcatcgaaca gaaaaacaac ggcgacggca gccgcgtcgt ccaatttaca cacatccatt
    10021 aaataacacc cgaaaaagaa agagcaaacc atgaccgtca aaacccccgt ttacctcgac
    10081 tacgccgcca ccacccccgt tgacaaacgc gttgccgaaa aaatgattcc ctatctgacc
    10141 gaaaccttcg gcaacccagc ctccaacagc cacagcttcg gctgggaagc agaagaagct
    10201 gtagaaaaag cacgtgcaga cattgccgcc ctgattaacg ccgactctaa agaaatcgtt
    10261 ttcaccagcg gcgcgaccga gtccgacaac ctcgccatca aaggcgcggc aaacttctac
    10321 aaaaccaaag gcaaacacct catcaccgtc aaaaccgaac acaaagccgt actcgacacc
    10381 atgcgcgaac tcgaacgcca aggttacgaa gtaacttatc tggacgtaca agaaaacggt
    10441 ttggttgatt tagacgtact gaaagccgcc atccgcgaag acaccatcct cgtttccgta
    10501 atgtgggtaa acaacgaaat cggcgtggtt caagatattc ccgccatcgg cgaaatctgc
    10561 cgcgaacgca aaatcatttt ccacgtcgat gccgcccaag cctgcggcaa agtacctgtt
    10621 gacgttgaag ccgccaaagt tgatttgttg tctatgtccg gccacaaagt atacggccct
    10681 aaaggcatcg gcgcgctgta cgtccgccgc aaaccgcgcg tccgcctcga agcccaaatg
    10741 cacggtggcg gccacgaacg cggcttccgc agcggcacat taccgaccca ccaaatcgtc
    10801 ggtatgggcg aagccttccg catcgccaaa gaagaactcg aacaagatat ggcgcactac
    10861 cgcaaactgc gcgacatctt cctcaaaggc atcgaaggca tcgaagaagt ctatatcaac
    10921 ggcgacctcg aacaccgcgc cccgaacaac ctgaacgtca gcttcaactt cgtcgaaggc
    10981 gaaagcctga ttatggcagt gaaagaactc gccgtatcca gcggctccgc ctgcacctcc
    11041 gcctcgctcg aacccagcta cgtcctgcgc gcattgggca gaaatgacga attggcgcac
    11101 tcgtccctgc gcatcacctt cggtcgcatg accactgaag aagaagtgca atttgccgcc
    11161 gaactgatta aatccaaaat cggcaaactg cgcgaactgt cgccgttgtg ggaaatgttc
    11221 aaagacggga ttgatttgaa ctcgattgaa tgggcggcgc attaaagcgt accaacatgc
    11281 catccgaacc ttcagacggc attccaaaaa caaagcaatc aagagaaaaa tatgaacgaa
    11341 caagatttag atttggacaa cctcgacaac ctgcttgacg atttcgacgg cgttaccgtg
    11401 gaaggcggcg tcgattcgga aaacgacgac ggctgtgaag gcggagcgtg caaaatctaa
    11461 ctcaggccgt ctgaaaaccg gcaaacaaac cacttaaatc atcaaaacat taaggaaacc
    11521 acatcatggc atacagcgat aaagtaatcg accactacga aaatccccgc aacgtcggta
    11581 ctttcgacaa aaacgacgaa tctgtcggca ccggcatggt cggcgcaccg gcctgcggcg
    11641 acgtgatgcg cctgcaaatc aaagtgaacg atgaaggcat catcgaagat gcgaaattca
    11701 aaacttacgg ctgcggttcc gccatcgctt cgtccagcct gattaccgag tgggtcaaag
    11761 gcaaaagcct ggatgacgcg ctggcaatca aaaacagcga aatcgccgag gagttggaac
    11821 tgccgccggt aaaaatccac tgctccatct tagcggaaga cgcggtaaaa gcggccgttg
    11881 ccgactaccg caaacgtcag gaaaacagat aaagcccttc agacggcatc gtcccgcaat
    11941 gccgtccgaa ccacccgccg cttcaggtcc gtccggggcg gtacaacaag gaagaaatat
    12001 gattaccctt accgagaatg ccgcaaaaca catcaatgac tatctcgcca aacgcggcaa
    12061 aggcttgggc gtacgcttgg gtgtgaaaac cagcggctgc tcggggatgg cgtacaacct
    12121 tgaatttgtc gatgaagccg atggcgacga cctgattttc gaaggacacg gcgcgcgcat
    12181 ttatatcgat ccgaaaagcc tggtttatct ggacggcacg caagtcgatt acaccaaaga
    12241 aggtttgcag gaaggtttca aatttgaaaa ccccaatgtc aaagactcct gcggctgcgg
    12301 cgaaagcttc cacgtttaag gcataaaaac ggcgggacag tatcaaaacc gtcccgccat
    12361 tttttcgctt cctgcctgtt gtagctgcct ttgcctttcc ttttccgttc caccttgtgc
    12421 cggaacaaat cggatttcac taaggctttt aaagcattgt cgcgtatttt gcctttattg
    12481 tgctgcactt tgccgcccat attcagtcct ttcgtttaag aagctgcaga ttataaggca
    12541 aaaacagttt tctgccaaaa tcttacattt atcatcctac tatgtcccaa tatttcaccc
    12601 tcttccggat tgaacccgct ttcgatatcg acaccgaaaa cttggaacaa acctaccgcg
    12661 ccttggccgc ccgtttccat cccgataaat tcgcttcagc ttccgccttt gagcaaaagc
    12721 aggcagtgat gatgtcttcc accatcaacg atgcctaccg caccttgaaa aaccccatcg
    12781 accgcgccgc ctacctgctg aaaaaatcgg gcatcgatgc cgacgcgccg gagcataccg
    12841 ctttcgcccc cgaattcctt atgcagcaaa tggaatggcg cgaaacgctg atggaggcac
    12901 gggcaggcaa cgaccttgaa tccttgaaaa atctcgacaa cgaaatccgc gacgagcaag
    12961 aaaaactgtt ctgcggtctg aaacagtcgt ttgcccgaca agattacgac acagccgcac
    13021 aacaagtccg ccaaggcagg tttctcgaca aactccgcaa cgaaatttcc tcggcattat
    13081 aatccgcacc gtgtttcaga cggcgtaacc gccgcaccgt tccgcgtcaa aatatgctaa
    13141 aataagcaac aattttttgc catacgaaac attgaaacca tgaccgacgc aaccatccgc
    13201 cacgaccaca aattcgccct cgaaaccctg ccggtaagcc ttgaagacga aatgcgcaaa
    13261 agctatctcg actacgccat gagcgtcatt gtcgggcgcg cgctgccgga cgttcgcgac
    13321 ggtctcaagc cggtacaccg ccgcgtactg tacgcgatgc acgaattgaa aaacaactgg
    13381 aatgccgcct acaaaaaatc ggcgcgcatt gtcggcgacg tcatcggtaa ataccacccc
    13441 cacggcgata ccgccgtata cgacaccatc gtccgtatgg cgcaaaattt cgctatgcgt
    13501 tatgtgctga tagacggaca gggcaacttc ggatcggtgg acgggcttgc cgccgcagcc
    13561 atgcgctaca ccgaaatccg catggcgaaa atttcccacg aaatgctggc agacattgag
    13621 gaagaaaccg tcaatttcgg cccgaactac gacggtagcg aacacgagcc gcttgtactg
    13681 ccgacccgtt tccccacact gctcgtcaac ggctcgtccg gcatcgccgt cggcatggcg
    13741 accaatatcc cgccgcacaa cctttccgat accgtcaatg cctgcctgcg cctgctcgat
    13801 gcacccgaca ccgaaatcga cgaactgatc gacattatcc aagcccccga cttcccgacc
    13861 ggggcaacca tctacggctt gagcggcgtg cgcgaaggct ataaaacagg ccgcggccgc
    13921 gtcgttatgc gcggtaagac ccatatcgaa cccataggca gaaacggcga acgcgaagcc
    13981 atcgttatcg acgaaatccc ctatcaggtc aacaaagcca agctggtcga gaaaatcggc
    14041 gatttggttc gggaaaaaac actggaaggc atttccgagc tccgcgacga atccgacaaa
    14101 tccggtatgc gcgtcgttat cgagctgaaa cgcaacgaaa atgccgaagt cgtcttaaac
    14161 caactctaca aactgactcc gctgcaagac agtttcggca tcaatatggt ggttttggtc
    14221 gacggacaac cgcgcctgtt gaacctgaaa cagattctct ccgaattcct gcgccaccgc
    14281 cgcgaagtcg ttacccgacg tacgcttttc cggctgaaga aggcacgcca tgaagggcat
    14341 atcgccgaag gcaaagccgt cgcactgtcc aatatcgatg aaatcatcaa gctcatcaaa
    14401 gaatcgccca acgcagccga ggccaaagac aaactgcttg cacgtccttg gcgcagcagc
    14461 ctcgttgaag aaatgctgac gcgttccggt ctggatttgg aaatgatgcg tccggaagga
    14521 ttggctgcaa acatcggctt gaaagagcaa ggttattacc tgagcgagat tcaggcagat
    14581 gctattttac gcatgagcct gcgaaacctg accggcctcg atcaagaaga aattgtcgaa
    14641 agctacaaaa acctgatggg taaaatcatc gactttgtgg atatcctctc caaacccgaa
    14701 cgcattaccc aaatcatccg cgacgaactg gaagaaatca aaaccaacta tggcgacgaa
    14761 cgccgcagcg aaatcaaccc gttcggtggc gacattgccg atgaagacct gattccgcaa
    14821 cgcgaaatgg tcgttaccct gacacatggc ggctatatca aaacccagcc gaccaccgac
    14881 tatcaggcgc agcgtcgcgg cgggcgcggc aaacaggcgg ctgccaccaa agacgaagac
    14941 tttatcgaaa ccctgtttgt tgccaacacg catgattatt tgatgtgctt taccaatttg
    15001 ggcaagtgtc attggattaa ggtttacaaa ctgcccgaag gcggacgcaa cagccgcggc
    15061 cgtccgatta acaacgtcat ccagttggaa gaaggcgaaa aagtcagcgc gattctggca
    15121 gtacgcgagt tccccgaaga ccaatacgtc ttcttcgcca ccgcgcaggg actggtgaaa
    15181 aaagtccaac tttccgcctt taaaaacgtc cgcgcccaag gcattaaagc catcgcgctc
    15241 aaagaaggcg actacctcgt cggcgctgcg caaacaggcg gtgcggacga catcatgctg
    15301 ttctccaact taggtaaagc catccgcttc aacgaatact gggaaaaatc cggcaacgac
    15361 gaagcggaag atgccgacat cgaaaccgaa atttcagacg gcatcgaaga tgaaaccgcc
    15421 gacagcgaaa acgcactgcc gagcggcaaa cacggtgttc gcccgtccgg tcgcggcagc
    15481 ggcggtttgc gcggtatgcg cctgcctgcc gacggcaaaa tcgtcagcct gattaccttc
    15541 gcccctgaaa ccgaagaaag cggtttgcaa gttttaaccg ccaccgccaa cggatacgga
    15601 aaacgcaccc cgattgccga ttacagccgc aaaaacaaag gcgggcaagg caatattgcc
    15661 attaacaccg gcgagcgaaa cggcgatttg gtcgccgcaa ccttggtcgg cgaaaccgac
    15721 gatttgatgc tgattaccag cggcggcgta cttatccgca ccaaagtcga acaaatccgc
    15781 gaaaccggcc gcgccgcagc aggcgtgaaa ctgattaact tggacgaagg cgaaaccttg
    15841 gtatcgctgg aacgtgttgc cgaagacgaa tccgaactct ccgacgcttc tgtaatttcc
    15901 aatgtaaccg aaccggaagt cgagaactga aaatcatctc ccgatgccgt ctgaagattc
    15961 agacggcatt tattttatcc ctcatccgtc atccagcttc tcacaatata gcggattata
    16021 gtcaattaaa aaca
//
LOCUS       DAWHRS010000031        16003 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWHRS010000031 DAWHRS010000000
VERSION     DAWHRS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.031"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..2985
                     /gene="mfd"
                     /locus_tag="WIB82_000696"
     CDS             <1..2985
                     /gene="mfd"
                     /locus_tag="WIB82_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ3842032.1"
                     /translation="VYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDALFVSLGD
                     VHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLPDVSGKE
                     HALPDLAVNRQSDEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGLKAKPVS
                     DWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTEFSAAAT
                     NPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSPVGEGWG
                     EGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIGDPVVHE
                     EHGIGRYMGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHENIALHK
                     LGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDYQAFADGFGYEET
                     EDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTL
                     LVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDDI
                     KFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSLI
                     TTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRERLETLLP
                     EARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKFG
                     LAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLEI
                     RGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPA
                     LLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIESHHLRL
                     MAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRFTAEMEN
                     IEVRINTVKNVLKTLQNRCLPK"
     gene            3134..3517
                     /locus_tag="WIB82_000697"
     CDS             3134..3517
                     /locus_tag="WIB82_000697"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ3842033.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            3539..4381
                     /gene="kdsA"
                     /locus_tag="WIB82_000698"
     CDS             3539..4381
                     /gene="kdsA"
                     /locus_tag="WIB82_000698"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ3842034.1"
                     /translation="MDIKINDITIGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            4396..4836
                     /locus_tag="WIB82_000699"
     CDS             4396..4836
                     /locus_tag="WIB82_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ3842035.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            4883..6169
                     /gene="eno"
                     /locus_tag="WIB82_000700"
     CDS             4883..6169
                     /gene="eno"
                     /locus_tag="WIB82_000700"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ3842036.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMTLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLIVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTSAEFAEYLEGLV
                     NEFPIVSIEDGMDENDWEGWKLLTEKLGSRVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            6183..6461
                     /gene="ftsB"
                     /locus_tag="WIB82_000701"
     CDS             6183..6461
                     /gene="ftsB"
                     /locus_tag="WIB82_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ3842037.1"
                     /translation="MKWVTVVLSVSLVCCQYSLWFGKGSIGRNNNLKNQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(6502..6792)
                     /locus_tag="WIB82_000702"
     CDS             complement(6502..6792)
                     /locus_tag="WIB82_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ3842038.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLVGEDEGLSDSLSK"
     gene            complement(6809..6925)
                     /locus_tag="WIB82_000703"
                     /pseudo
     CDS             complement(6809..6925)
                     /locus_tag="WIB82_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7026..8159)
                     /gene="nrdB"
                     /locus_tag="WIB82_000704"
     CDS             complement(7026..8159)
                     /gene="nrdB"
                     /locus_tag="WIB82_000704"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ3842039.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(8220..9407)
                     /locus_tag="WIB82_000705"
     CDS             complement(8220..9407)
                     /locus_tag="WIB82_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ3842040.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(9400..10413)
                     /gene="dcm"
                     /locus_tag="WIB82_000706"
     CDS             complement(9400..10413)
                     /gene="dcm"
                     /locus_tag="WIB82_000706"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3842041.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(10471..12750)
                     /gene="nrdA"
                     /locus_tag="WIB82_000707"
     CDS             complement(10471..12750)
                     /gene="nrdA"
                     /locus_tag="WIB82_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ3842042.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            13279..13590
                     /locus_tag="WIB82_000708"
     CDS             13279..13590
                     /locus_tag="WIB82_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ3842043.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
     gene            complement(13901..14668)
                     /locus_tag="WIB82_000709"
     CDS             complement(13901..14668)
                     /locus_tag="WIB82_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ3842044.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIRV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(14756..15583)
                     /gene="mutM"
                     /locus_tag="WIB82_000710"
     CDS             complement(14756..15583)
                     /gene="mutM"
                     /locus_tag="WIB82_000710"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ3842045.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPRCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(15635..>16003)
                     /locus_tag="WIB82_000711"
     CDS             complement(15635..>16003)
                     /locus_tag="WIB82_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3842046.1"
                     /translation="GRLMLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADV
                     GFDIEFGKFMVYLPPVSSLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLP
                     AWDGYLGGKALAAGAARVAD"
BASE COUNT         4146 a         4292 c         3643 g         3922 t
ORIGIN      
        1 gtgtacaaag ccgtcagcaa cggccacttc ggcgcgggcg tggaatatta tctgccgctg
       61 ttttttgaaa atgagctgga aacgctgttt gactatatcg gcgaagacgc gctgtttgtc
      121 tctttgggcg atgttcacgc cgaggcaaac cgcttttgga gcgatgtcaa atcccgttac
      181 gcgatggcgc agggcgacga aacctatccg cctttgcttc cacagcattt gtatctctct
      241 gctgatgttt ttgcaggccg tctgaaaaac tacggacaag tgctgcccga tgtttccggc
      301 aaggaacacg ccctccccga ccttgccgtc aaccgacaat ccgacgagcc tttgcaggca
      361 ttgaaggatt ttcagacggc gtttgaagga cggattttgc tgtgcgccga aagtttggga
      421 cggcgcgaaa ccatgctcgg tttcttgcag caaaacggtt tgaaagccaa acctgtgtcc
      481 gactggcagg gcttcttgtc ggcgcatgag ccgctgatga ttacagttgc gccgttggca
      541 tacgggttca aactgggcgg actgcaatcg ccgaaccaac agcaaactac ttccgcctcc
      601 gaaggagaag gcgatgcggt taccgaccaa accgaatttt ccgcagccgc aacaaaccct
      661 ctccctagcc ctctcccaca ggagagggaa caaagtgcag ccgtcgtttc agacagtctg
      721 aaagcagccg ccgtttcaac cgaaagcagc ctgccccccg gtaaaagtaa tctgcacggg
      781 caaatccaac agcaacctgc cccctccccc gtgggggagg gttggggaga gggcaaagca
      841 gttgccgctc aaagtgccat cgccgtcatc accgaatccg atctctacca atacgtcgcc
      901 cgttcgcgcg tccacaaccg ccgcaagaaa cacgccgccg tttcagacgg tctgttgcgc
      961 gaccttgccg aaatcaacat cggcgacccc gtcgttcacg aagaacacgg catcgggcgg
     1021 tatatgggct tggtaacgat ggacttgggc ggcgaaacca acgaaatgat gttgctcgaa
     1081 tacgcaggcg aagcgcagct ttatgtgcct gtttcgcaac tgcatttaat cagccgctac
     1141 tccggtcagg cgcatgaaaa cattgccctg cacaagctcg gcagtggcgc gtggaacaag
     1201 gcgaagcgca aagccgccga aaaggcgcgc gataccgccg ccgagttact taacctttac
     1261 gcccgacgtg ccgcccaatc gggacacaag tttgaaatca acgagttgga ttatcaggcg
     1321 tttgccgacg gcttcggcta cgaagaaacc gaagaccagg cagccgccat cgccgccgtg
     1381 attaaagatt tgacgcaggc gaagccgatg gaccgccttg tgtgcggcga tgtcggcttc
     1441 ggcaaaaccg aagttgccct gcgcgccgca tttgtggcgg tgatgggcgg caaacaggtc
     1501 gccgtactcg ccccgaccac gcttctggtc gagcagcacg cgcaaaactt tgccgaccgt
     1561 ttcgccgatt tccctgtgaa agtcgctagc ctttcgcgtt tcaacaacag caaagccact
     1621 aaagccgcgc tggaaggcat ggcggacggc acggtcgaca tcgttatcgg cacgcacaaa
     1681 ctggtgcagg acgacatcaa attcaaaaac ttaggtttag tgattatcga cgaagaacac
     1741 cgcttcggtg tgcgccaaaa agagcagctc aaacgcctgc gcgccaatgt cgatatcctc
     1801 accatgaccg ccacgccgat tccgcgcacc ctcagcatgg cgctcgaagg tctgcgcgac
     1861 ttctcgctga ttactaccgc gccgagccgc cgcctcgccg tcaaaacttt tgtcaaaccc
     1921 ttcagcgaag gcagcgtgcg cgaagccgta ttgcgcgaac tcaaacgcgg cggacaagtg
     1981 tttttcctgc acaatgaagt agatacgatt gaaaatatgc gcgagcggct ggaaaccctg
     2041 ctgcccgaag cccgcatcgg cgtggcgcac ggacaactgc gcgagcgcga gctggaacaa
     2101 gtgatgcgcg actttttgca gcaaagattt aacgtgttgc tctgctccac catcatcgaa
     2161 accggcatcg acatccccaa cgccaacacc atcatcatca accgcgccga caaattcggg
     2221 ctggcgcaac tgcaccagct tcgcgggcgc gtcggccgca gccaccatca agcctacgcc
     2281 tacctgctca cgcccgaata catcaccaaa gacgcagaaa aacgcctcga cgccatcgcg
     2341 gcagcagacg aactcggcgc aggtttcacc ctcgccatgc aggatttgga aatccgcggc
     2401 gcaggcgaaa tcctcggcga agggcagtcc ggcgaaatga tacaggtcgg cttcacgctc
     2461 tacaccgaaa tgctcaaaca agccgtgcgc gacctcaaaa aaggccgcca gcccgacctc
     2521 gacgcaccgc tgggcatcac caccgaaatc aaactgcaca gccccgccct gctgcccgaa
     2581 agctactgcc ccgacatcca cgagcgtctc gtcctctaca aacgcctcgc cgtctgcgaa
     2641 accgtgcagc aaatcaacgc catacacgaa gaactcgtcg accgcttcgg cctgcccgaa
     2701 caacccgtca aaacccttat cgaaagccac cacttacggc ttatggcaaa agaattgggt
     2761 atcgatgcca ttgatgcggc cggcgaagcg gtaacggtaa ccttcggtaa aaacaataat
     2821 gtcgatccaa ccgaaatcat cctgctgatt cagaacgaca aaaaataccg ccttgccggc
     2881 gccgataagc tgcggtttac cgcagagatg gaaaatatcg aggtcagaat caacaccgta
     2941 aaaaacgttt taaaaacctt gcaaaacaga tgcctgccca aataaagccg acaccgcaat
     3001 gccgtctgaa acaccgtttt ccttgtccga aagccgccat tatgaatttg aaggaaactc
     3061 cactataata cggcattcag atttccagac ggcatcgcgc ccgtcagacc gcacacaaac
     3121 caaaaggaaa tacatgttcc gtactatgct tggcggaaaa atccaccgcg ccaccgtaac
     3181 cgaagccgat ttgaactacg tcggcagcat taccgtcgat caagacctgt tggacgcggc
     3241 aggcatctat cccaacgaaa aagtcgccat cgtcaacaac aacaacggcg aacgttttga
     3301 aacctatacc atttcaggca aacgcggcag cggcgtgatt tgtctgaacg gtgctgcggc
     3361 aaggctggtg cagaaaggcg atatcgtcat catcatgtct tacgtccaac tctccgaacc
     3421 cgaaatcgcc gcacacgagc ccaaagtcgt cttggtagac ggaaacaaca aaatccgcga
     3481 catcatctcc tacgagccgc cgcacaccgt actgtaaccc tgaaaacgga cattgattat
     3541 ggatattaaa atcaacgaca tcaccatcgg caacaactcg cccttcgtcc tattcggcgg
     3601 catcaacgtt ttggaaagct tggattccac cctccaaacc tgcgcgcatt acgtcgaagt
     3661 aacccgcaaa ctcggaattc cctatatctt taaagcctct ttcgacaagg caaaccgttc
     3721 ctccatccat tcttatcgcg gcgtaggctt ggaagaaggc ttaaagattt ttgaaaaagt
     3781 caaagcagag ttcggcatcc ccgtcattac cgacgtacac gaaccccatc agtgccaacc
     3841 cgtcgccgaa gtgtgcgatg tcatccagct tcccgccttt cttgcgcggc agaccgattt
     3901 agtggttgcc atggcaaaaa ctggcaacgt cgtcaacatc aaaaaacctc agttcctcag
     3961 cccctctcaa atgaaaaaca ttgtggaaaa attccacgaa gccggcaacg ggaaactgat
     4021 tttatgcgaa cgcggcagca gcttcggcta cgacaacctc gttgtcgata tgctcggttt
     4081 cggcgtgatg aaacaaacct gcggcaacct gccggttatt ttcgacgtta cccattccct
     4141 gcaaacccgc gatgctggtt ctgccgcatc cggcggtcgt cgcgcacagg ctttggattt
     4201 ggcacttgca ggcatggcaa cccgccttgc cggtctgttc ctcgaatcgc accccgatcc
     4261 gaaactggca aaatgcgacg gccccagcgc gctgccgctg caccttttag aagatttttt
     4321 aatccgcatc aaagcattgg acgatttaat caaatcacaa ccgattttaa caatcgagta
     4381 acacggtttc gccttatgat gcagactttc cgaaaaatca gcctgtatgc cgcaaccttg
     4441 tggctcggta tgcagattat ggcgggttat atcgtcgcgc cggtgctgtt caaaatgctg
     4501 cccaaaatgc aggcgggcga aattgccggc gtattgttcg acatcctctc ttggagcggg
     4561 cttgccgttt ggggcgcggt actggctgcc gcctttgccg ccctaacccg gcggcaaacc
     4621 gccctgctgc tttttttatt gtccgccctt gccgccaacc gattcttgat tacacccgtt
     4681 atcgaggcac tgaaatacgg acatgaaaat tggctgttgt cgtttgtagg cggatccttc
     4741 ggaatgtggc acggcatttc cagtattgtt tttatggcaa ccgccctact ttcagcagtt
     4801 ttaagttggc ggctttccgg caaagatgcc gtctgaagcc ctcccatttt ttacctccct
     4861 tcacttcact tggagaacat tcatgagcgc aatcgttgat attttcgccc gcgaaatttt
     4921 ggactcacgc ggcaacccca cagtcgagtg tgatgtattg ctcgaatccg gcgtaatggg
     4981 acgcgcagcc gtaccgagcg gcgcgtccac cggtcaaaaa gaggctttgg aacttcgcga
     5041 cggcgacaaa tcccgctatt ccggcaaggg cgtattgaag gcggtcgaac acgtcaacaa
     5101 ccaaatcgcc caagccctca tcggtatcga tgccaacgag caatcttata tcgaccaaat
     5161 catgatcgaa ttggacggta ctgaaaacaa aggcaatttg ggtgcgaatg cgactttggc
     5221 ggtttctatg gcggttgcac gcgccgctgc cgaagactca ggcctgccgc tttaccgcta
     5281 cttgggcggc gcaggcccaa tgaccctgcc tgttccgatg atgaacgtta tcaacggcgg
     5341 cgaacatgcc aataacagcc tgaatatcca agaattcatg attatgcctg tcggcgcaaa
     5401 atctttccgc gaagccctgc gttgcggtgc cgaaatcttc cacgcactga aaaaactgtg
     5461 cgacagcaaa ggcttcccaa ctaccgttgg tgacgaaggc ggtttcgcac ctaacctgaa
     5521 cagccacaaa gaagccctgc aactgatcgt cgaagcaact gaagccgcag gctataaagc
     5581 aggcgaagac gtattgttcg ccctggactg cgcatccagc gaattctaca aagacggcaa
     5641 ataccacttg gaagccgaag gccgctctta caccagcgca gaatttgccg aatacttgga
     5701 aggcttggtt aacgaattcc caatcgtttc catcgaagac ggcatggatg aaaacgactg
     5761 ggaaggctgg aaactgctga ccgaaaaatt gggcagcaga gttcaattgg tcggcgacga
     5821 cttgttcgta accaatccaa aaatcttggc cgaaggcatc gaaaaaggcg tggcaaacgc
     5881 actattggtc aaagtcaacc aaatcggtac tttgagcgaa accctgaaag ccgtcgactt
     5941 ggccaaacgt aaccgctacg ccagcgtaat gagccaccgc tccggcgaaa ccgaagacag
     6001 taccattgcc gacttggcag tagccaccaa ctgtatgcag atcaaaaccg gctccctgtc
     6061 ccgttccgac cgcatggcaa aatacaacca actgctgcgt atcgaggaag aattggcgga
     6121 agccgcctac taccctggga aagccgcatt ctaccaactg ggcaaataaa aaaggttaac
     6181 gtatgaagtg ggtaactgtc gttttatccg tctcacttgt ctgctgccag tacagcctct
     6241 ggttcggcaa aggcagtatc gggcgcaaca acaacctgaa aaatcagatt gccgttcagg
     6301 aagaaaaaaa ccagacactc gccctacgca accattccct tgcagccgaa gtctatgatt
     6361 tggaaaacgg tcaagaagcc atttcggaaa tcgcccgggt agaactgggt tatatccaag
     6421 acggcgaaac cttttaccga ctcatcaggc ataaccggta ataccgtcaa aaagccgtcc
     6481 gaaccaatgt tcggacggct tttatttcga caaactgtca gacagcccct catcctcccc
     6541 caccaaaccg caatccagcc tgacatcccc ctcgacgcaa cagcagcacg gcagtatctc
     6601 gtcccgcccc aaaaaagcca aaggcggctc ccgataagta acgcttccct ccaaaatctt
     6661 cacccggcac gatccgcaat atccgcttcg gcactgatat tccaccatat gccccgtccg
     6721 ttccaagcct tccaacagag tctcgccctc caagagttca aaaaaaccct tattcgtacc
     6781 aatgcgcgcc atttccgacc aatcaaaaat atagtggatt aaatttaaac cagtacggcg
     6841 ttgcctcgct ttgccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt
     6901 tgttaatcca ctataatcca ctataaaagg aacaataacc gatcctaccc gctgtttttc
     6961 ccatcataca acatacaaat gccgtctgaa acatccggct tcagacggca ttttttcaaa
     7021 aaacattaca actcaaaatc gcccaaatca tccgtattca cttcagaatc tatctgaccg
     7081 atcaaataag aggatatttc cacttcctgc ggcgcgacct gtacgttgtc ggacgacagc
     7141 cacgcattaa tccacggaat cgggttttga tttgcgcctt caaatccggc cggcaacccc
     7201 accgcctgca tacgcagatt ggtaatatat tcgacgtatt gggataagat ttctttgttc
     7261 aaaccaatca tcgaaccgtc tttaaacaaa tatgccgccc attctttttc ctgttccgcc
     7321 gcttttttga agagttggaa acattcgtcc tgcaactcgg cggcaatttc tgccatttca
     7381 gaatcatcaa cacccgaacg catcagatta agcatatgct gcgtgccggt caggtgcagg
     7441 gcttcgtcgc gggcaatcag tttgatgatt ttggcgttgc cttccatcaa ctcgcgctcg
     7501 gcaaaagcaa acgagcaggc gaatgaaaca tagaaacgga tagcttccaa cacgttgacg
     7561 cacatcaggc agagatagag ttttttcttc aacccgcgca aagacacagt aatgggtttt
     7621 ccgccgacat tgtgcacccc ttcgcccaac agattgtaat actgggtgta ttcgattaag
     7681 tcgtcgtaat agcaggcaat gtcttcggcg cgggcggtaa tgtattcgtt ttcgacaata
     7741 tcatcaaaca cgaccgacgg atcattcaca atattgcgga tgatgtgggt atagctgcgc
     7801 gagtgtatgg tttcgctgaa gctccacgtt tcaatccacg tttccaactc gggaatcgaa
     7861 accaaaggca gcaaggcaac attcggactg cgcccttgga tggaatcgag cagtgtttgg
     7921 tatttcagat tgctgatgaa aatatgtttt tcgtgttcgg gcagattggc gtagtcgata
     7981 cggtcgcgcg agacatcgat ttcttcaggc cgccagaaga aagacaattg tttttcaatc
     8041 agtttttcaa atacttcgta tttctgctgg tcataacggg caacattaac cggctgacca
     8101 aaaaacatcg gctcattcag cgcgtcgttt ttggttttgg gaaaggtgct gtatgacata
     8161 gttaagtgtt cgcaggacat aatctattct ctatattaat taaaaatgat ctgtaagttt
     8221 taactaacct gctgagactt tatatattta ataaaatttt ctaaagttaa aacatgggtt
     8281 ttcccaaaag ttccaaatgt ttttacggat tctttaggga caactaaggt taaattctca
     8341 tcactcattt cctgaatttg agtatcagat ataccttctt gtagagtaaa taaatgctta
     8401 tgaggaattc gatctgcttc atttaaaact tggcgccagc gatccttaca agtagtcttt
     8461 gaacctagca ttgtcagctt ttcagtttga aatatgaaat tccctgattc atccatcgca
     8521 tggtactctt cacttccagg aaataaaaaa tctggtttct ttttcccttc tgtcttcgct
     8581 tgcgtctcaa attttaagga aaattcgtca aatattcttg ataaatgtaa ctctaaagat
     8641 tttcctgctc ttgcttttct gcgatttgta aaagaatgtg caaaattaat gaagctatcc
     8701 aaatcagtaa tttttgattt aataatttca aattcacgtt tttcaaaaat ggaaaataat
     8761 tcatattcct ttgaaaccca ttgttttaac acattactaa cgttagtttt atttaatgca
     8821 atattttccc ttgccaaaac tgccatttct tcagttttgg ggaagttagg aaatagtgaa
     8881 aataattttg ttaggttatc ctctacttct tgttttttag gtaaatataa acttcctggt
     8941 aaaatgttag tttctgcgat aaatatttca atatcttcat ctgaagataa aacaaaagca
     9001 tggaataata aatctttata acaagctctg cacaatacca ataaggaacc actatattta
     9061 tcacttaaaa attcaaaatt cttgccaaaa cccgtaattc tcgcctcatt tctcgtgcct
     9121 tggccgtaat atttaaaatt gctattagtg acgcttccat cttgccaatt aattttgatt
     9181 gaaatagttt tatttgttcc cttttggcaa actacatcaa agaggtcgtc tgaaaaatgt
     9241 ttttgaatat aaaatcctga ttggtgacta ccagtagtac caacatcatt gggacgaata
     9301 taacgacagt atactgcaat tgatttattt gctgtctgta ttgctgaagc tactaagtca
     9361 tcggatttat ttactacttg tattgtctga gctgccaagt cattcatttt ttaactcatt
     9421 tataatttgt ttagcaatag cttgaaccaa cggtacagca attgaattgc caaactgctt
     9481 gtatgcagct gtcttggata ctgcatcaat aacaaaatct ttaggaaatc ccattaaacg
     9541 cgagcactcc ctaggtgtca gcttcctagg attttttcct ttctgaggga tgagtatttc
     9601 ggaaccatct ttgtaatatc gtgcagatag agttcgtgat attccatcta aatcaactaa
     9661 tccaaaacca aatccattac cctttgcctt atgtttttta gcgtaatttt gaaggtaaag
     9721 ccataagtta tcagaaagag taaaagaatt atctacatca tcttccaaaa tttgctttaa
     9781 ttttggttgt gattctggtg gggaaggaaa attgaaattt atttccttat taaaatattg
     9841 tctatcaaaa cctacaataa aaatacgctc cctattttga ggaacataat attttgcatt
     9901 cataacttga taaaatatct gatagtcaag ctcttctaaa gtccctttaa ttactttaaa
     9961 tgtatttcct ttgtcatgcg aaacaaggtt tttcacattc tctaaaagaa aaattttagg
    10021 tcgatgtttt ccaataattt cagcaacatc aaaaaataga gttccctgcg ccttatctaa
    10081 gaagcctgtt tctcgtccta ggcttttttt ctttgaaaca ccagctatag agaatggctg
    10141 acacgggaat cctgctgtta atacatcaaa cttacttgga atagctgctt tggtttcctt
    10201 taatgtaata tctccataag gaatatcatt aaaatttact tggtaggttt gacgggcttt
    10261 atcatcccat tcactagaaa atacacatcg cccaccaaca ttctccattg caatgcgaaa
    10321 accacctatt cccgcaaata aatcaataaa agtaaatttc tcattattta aggtaactat
    10381 attatctaat tgattttgta ttttattttt catattttat atttcagatg atttattaat
    10441 ctaaaggcca tctgaaaact ccccctttca tcaaatctta caagccccgc ccgcgcagcc
    10501 gtcatcctga atatcggtct gcgtatcgtc cgcaccgtcg cgggtgttat ggtagtacag
    10561 ggttttgacg ccgtatttgt aggcggtcag caggtctttg agcatttgtt tcatagaaac
    10621 tttgccgcct tcgaatttgc ccgggtcgta ggcggtattg gcggaaatcg attgatcgac
    10681 gaatttttgc atcacgccga caagtttcag gtagccttcg ttgccgggaa gctgccacag
    10741 ggtttcatag gcatttttca gggtttcaaa ctccggcacg acttgtttca aaatgccgtc
    10801 tttcgatgct ttgaccgtta ccaatccgcg cggcggctcg atgccgttgg tggcgttggc
    10861 gatttgagag ctggtttcag acggcatgag cgcggtcaga gtagagttgc gcaggccgta
    10921 tttgacgatt tcggcacgca ggctttccca gtcgtaatgc aaaggctcgc cgcagacggc
    10981 atccaaatct tttttgtaag tgtcgatggg cagtttgcct tgcgaataaa cggtttggtt
    11041 aaagagcgtg cacgcaccgt attctttggc aaggtttgcc gatgctttga gcaggtaata
    11101 ctgtatggct tcaaaggtac ggtgggtcag accgagcgcg gaaccgtcgc tgtagcggac
    11161 accgtttttc gccagataat aagcatagtt aatcacgccg atgccgagcg aacggcggcc
    11221 catagtagag gtacgcgcgg cttctaccgg atatccctga taatctaaaa gtgcatcgag
    11281 cgcacgaacg gtcaagtcgg caagcccttc caattcgtcc aagctgttta atgcgcccaa
    11341 gttaaaggca gacagtgtac acagggcgat ttcgccgttc ggatcgttga tattgtccag
    11401 cggtttggtc ggcagggcga tttccataca caagttggac tgatgaacag gcgcgacgcg
    11461 cggatcgaac gggctgtgcg tattgcagtg atcgacgttt tgaatgtaga tgcgcccggt
    11521 tccggcacgc tcctgcatca gcgtggaaaa caggtcggca gccggaatga tgcgcttgcg
    11581 gatatcaggg tcttgctcgt atttcgtata gagccgctca aattcgtctt ggtcggcaaa
    11641 aaacgcttcg tacaatcccg gaacctcgtt gggcgaaaac agcgtaatgt tgccgccctt
    11701 aatcaggcgg gtgtacagca ggcggttgat ttgcacgccg taatcaagct gacggatacg
    11761 gttgtcttcc acaccgcggt tgtttttcaa caccagcagg ctttcggctt cgatatgcca
    11821 caaggggtag aacaaggttg ccgcgccgcc gcgcacgccg ccttgcgaac aggatttgac
    11881 cgccgcctga aacattttaa agaagggaat gcagccggta tgccgcgctt cgccgccccg
    11941 gatttcgctg tccaaaccgc ggatacgtcc ggcattgatg ccgatgcccg cacgctggga
    12001 aacgtatttc acaatcgcgc tggtagtggc attgatggaa tccaaactat cgtcgcattc
    12061 aatcagcaca cagcttgaga actggcgcgt aggcgtacgc acgccgctca taatcggagt
    12121 cggcagcgat actttaaatg tagaaacggc atcgtaaaac cgtttgacgt aacccaagcg
    12181 cgcctctttc gggtatttgc tgaaaaggca catcgccacc aaaacatata aaaactgcgg
    12241 cgtttcgtaa atttggcggg taacgcggtt ctgtaccaga tatttgcctt cgagctgttt
    12301 gacagcggca taggaaaagg acatatcgcg ttcgtggtcg atataggcgt tcagttcgtc
    12361 aaattcttcg cggctgtaat cctcaaggat atgcctgtcg tattttccgg catcggtaag
    12421 ttttttaacg tggtcgtaaa ggtgcggcgg ctcgtactcg ccgtaggcta ttttacgaag
    12481 atggaaaatc gccaaacgcg cggcaaggta ttggtagtcc ggggtatctt ccgaaattaa
    12541 atcggcagcg gctttgatga tggtttcgtg gatgtcgtcg gtgcggatgc cgttgtagaa
    12601 ctggatgtgc gatttcaact cgacctgcga cacggaaaca ttttccaatc cgtccgccgc
    12661 ccaagtgacg acacggtgaa tcttatccaa atcaatggct tctaatcttc cgtctcgttt
    12721 ggttactttc aaatcagtcg gtgtattcat cgcttcctct tccactcttg atattcaaga
    12781 cacagtcttt tcaaataaat taaggcagac aatatagtgg atttttggca ttttctccag
    12841 tcttgacaac ggttgtattt ttcagtttca ggcaatgcgc gataaaaccc gcctgtcgta
    12901 tttttcctat aatatttgtt ttatctgata attctttacc gataaaaaac gggtaaattt
    12961 tttgcctttt gaccggctcc ggctacaagg cggtgaaata aggattttcc gacgaaaaag
    13021 gaaagcttcc tgttttctgc ccctgcaaat tgttaaattt tgcaaagtat gattttgccg
    13081 cgccgccgcc gacaaattcc attttcttac cgattggaat ttattattga gattaatgtg
    13141 ttatttgaat ctgcatatca aacggcaagt tttgtcggct gaatgagtct gaaactgccg
    13201 acaatttgcc cgttccatcc ttccatccta tactggaaag aatgacaaac tgaaaggatc
    13261 gtcatgtctg tcaaaaagat gcttgccata ctgttgtctg caatattggg actggtatcg
    13321 acaactgccg ctgccggtac gtcagaaccc gcccaccgcg ataccaaaca tatccgcaag
    13381 gcaaacaagc agatgctgca ccccgaatgc aggaaatatt tggaacgccg tgccgcgtgg
    13441 taccgatcgc aaggcaacgt gcaggaattg cgcgaaaaca aaaaggcgcg caaagcattc
    13501 cgctccctgc cttatgcgga acagaaaatc caatgccggg cggcttatga ggctttcgat
    13561 gatttcgacg gcggcagttt ccgccgttaa tcccatataa aatatgccgt ctgaacacag
    13621 gttcagacgg cattttccat agatacaaca aactatcctt acgccatttc gtttgaggtc
    13681 ctcccgttgc cgcagcctta atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
    13741 cgcagacagt acagatagta cgaaaccgat tcacttggtg cttcagcacc ttagagaatc
    13801 gttctctttg agctaaggcg aggcaacgct gtactggttt ttgttaatcc actatacatc
    13861 cacaaaagcg aatcatactg ccacattccg acaaccggtt tcagacagcg atatccgcat
    13921 cgtcggcgac aacggcacgg atgctttctt cgattttatc ttttaaagca taacggtctt
    13981 cgctttccgc cgcatccgcc acgcaaacga aatcgactct tatcgtcaat tttttcatag
    14041 acacgatgcg ccacaggcag gtcggcaaac cgacatcggc atatgaggga cgagccgtcc
    14101 tttttcccgt ttcgtcataa taacgcagcg cgaccgccaa aacctttgcc cccgcatcga
    14161 tggcggattg gaacagcgcg gctttgaacg gcaaaagccc caatccggag gaagtccgcg
    14221 cttcggggaa aaaactgacg ttttgaccgc gttgcaaggt ttcgcagacg gcgcggttaa
    14281 tcggttcgat gtcgcgccgc gaattgcggt tgatgaacac cgttcccgcg ttctgcccca
    14341 tcttgcccaa taccggccag cttttgattt cctgcttggc gataaagctg ctcggataaa
    14401 ccgcgctcat cgcgaaaata tccagccagg acacgtggtt ggcggcaacc aagacaccgt
    14461 tcggatgttc gggtgcgggt ctgcccacct ccaatccgat atccaaagcc gccaaaaccc
    14521 ccctgcccaa ctcgattacc gcccgattgc gcgactcggg gcaaccgccg tcaataccgc
    14581 gcaggttttt cccggttttg aacagccaga ccgccaaacg gcacaagcgg cgcagacgtg
    14641 taaaaaatga agctttattt gaagacatga gcaattcttt cccgataatc ggtgtttgtt
    14701 gttgaaacgt tcggacggca taccgataaa atgccgtctg aaaccgtttt cagtcctatt
    14761 tctgacagtt cgggcaataa aacgtgccgc gctgccccaa agtttctttc acaaccaaac
    14821 cgccgcaccg ggggcacggc tgattgtgcc gcccgtacac tgtatattcc tgttggaagt
    14881 agccgctttt gccgtcgctg tccacaaaat ccctcaaggt actgccgccc gtttcaatgg
    14941 cgcgctgcaa caccgctttg acggtttcaa ccaaaagcgc gcactctttc tttttcaggc
    15001 ggttggcagg acggtggggc gaaatgcccg ctctgaacag gctctcgttg gcataaatgt
    15061 tgcccacacc gaccacgacc gcattgtcca tcagggcaag tttgaccgcg cgcttctgcg
    15121 ccttcagcct tgcatacaga taatccgcac aaaatgcctc cgacaaaggc tccggcccca
    15181 gtttttccaa cagcggatga tgttcttcga ttccctcata ccaaagtatc gcgccgaact
    15241 ttctcggatc gcggtaacgc atgaccgtgc cgtctgaaaa cacaatatcg acgtgatcgt
    15301 gtctgtccgg cctgccgata cgtccgtccg acggcgtaaa aatccgcaag ctgcccgaca
    15361 tccccaagtg aatcagcagc acgcccgttt gaaagcggat aagcaggtat ttcgccctcc
    15421 tgccgcagga caacacctgc cggccggaca aaatctcccc caaatcggga ttaatctgcc
    15481 agcgcagctt caattggcgc aataccacgg cttccaccgt tttcccttca atatgcggcg
    15541 cgatgccgcg caacgtcgtt tccacttccg gcaattcagg cataacccct cccgacattt
    15601 cttctgacag atgccgtctg aaagacggct gtccctaatc cgcaaccctt gccgcacccg
    15661 ccgcaagggc tttgccgccc aaatacccgt cccacgcggg caggggcgtt acgcccgctg
    15721 cccttttgac caaaaccaaa ccgtacactg cggcacactg cggccaccaa cggtcgcccg
    15781 ccttttccat aaaccgccaa aagcgtattt gcccgagcga cgaaaccggc ggcagataca
    15841 ccataaattt cccaaattca atatcgaaac cgacatccgc aagccgtctt ttcaactcgg
    15901 gcagcggcag acaaaaccgt ttttccggca ggcgttcgcc gtcaaaccaa cggctgaatc
    15961 cccagagcga atacggattg aaacccgtca gcatcaagcg tcc
//
LOCUS       DAWHRS010000032        15982 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWHRS010000032 DAWHRS010000000
VERSION     DAWHRS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15982
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.032"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(311..733)
                     /locus_tag="WIB82_000712"
     CDS             complement(311..733)
                     /locus_tag="WIB82_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ3842047.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(851..1522)
                     /locus_tag="WIB82_000713"
     CDS             complement(851..1522)
                     /locus_tag="WIB82_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ3842048.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            complement(1538..2164)
                     /locus_tag="WIB82_000714"
     CDS             complement(1538..2164)
                     /locus_tag="WIB82_000714"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ3842049.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIHNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFTSRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2271..3089)
                     /locus_tag="WIB82_000715"
     CDS             complement(2271..3089)
                     /locus_tag="WIB82_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3842050.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3348..3788)
                     /locus_tag="WIB82_000716"
     CDS             complement(3348..3788)
                     /locus_tag="WIB82_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ3842051.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3851..5257)
                     /locus_tag="WIB82_000717"
     CDS             complement(3851..5257)
                     /locus_tag="WIB82_000717"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ3842052.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5427..6542
                     /gene="lptF"
                     /locus_tag="WIB82_000718"
     CDS             5427..6542
                     /gene="lptF"
                     /locus_tag="WIB82_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ3842053.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6539..7609
                     /gene="lptG"
                     /locus_tag="WIB82_000719"
     CDS             6539..7609
                     /gene="lptG"
                     /locus_tag="WIB82_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ3842054.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7819..10404
                     /gene="acnB"
                     /locus_tag="WIB82_000720"
     CDS             7819..10404
                     /gene="acnB"
                     /locus_tag="WIB82_000720"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ3842055.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLASIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10546..11541
                     /locus_tag="WIB82_000721"
     CDS             10546..11541
                     /locus_tag="WIB82_000721"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ3842056.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11610..11711)
                     /locus_tag="WIB82_000722"
                     /pseudo
     CDS             complement(11610..11711)
                     /locus_tag="WIB82_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11765..12778)
                     /gene="ilvC"
                     /locus_tag="WIB82_000723"
     CDS             complement(11765..12778)
                     /gene="ilvC"
                     /locus_tag="WIB82_000723"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ3842057.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGYVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12858..13151)
                     /locus_tag="WIB82_000724"
     CDS             complement(12858..13151)
                     /locus_tag="WIB82_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ3842058.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            complement(13218..13709)
                     /gene="ilvN"
                     /locus_tag="WIB82_000725"
     CDS             complement(13218..13709)
                     /gene="ilvN"
                     /locus_tag="WIB82_000725"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ3842059.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13720..15447)
                     /gene="ilvB"
                     /locus_tag="WIB82_000726"
     CDS             complement(13720..15447)
                     /gene="ilvB"
                     /locus_tag="WIB82_000726"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ3842060.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSSDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADGKA"
BASE COUNT         3751 a         4327 c         4062 g         3842 t
ORIGIN      
        1 ttcaataaat taatatatgg cttaaaataa cgggattctc gcctcccgcc cgcccgcaga
       61 agcaggcggg tatcatttta aaacgcggca tttaaaattt gaccgaaaat tgttgacaat
      121 ccggaatcaa gtctgcacaa taccccgaca agtccaagta ttataaaggc tgaataaaga
      181 ggaaacagca ggcagatata ttcgggaggt gcagtccgaa tatatctgct tttttatgcg
      241 cctccggatt gcctgccgca cctttccctt cagacggtat cagccgtttc cccataatgc
      301 cgcccgatgc ctatttatct gccccggcaa tttcaaaact gtgggtaatc tttgccgctt
      361 tgcccaacat aatcgaagcc gaacagtatt tttcggcaga catctgaacg gcgcgctcaa
      421 tggccgattc tttcaaatca tgcccgaata ctttgaaatg gatgtggatt tcggtaaaca
      481 cgcgcggcgc atcgtccgcc cgtttcgccg taaccgtcgc acggcagtca gtcactttct
      541 gacgctgttt ttcggcaatc atcaccacat cgatgctcga acagcccgcc acgcccaaca
      601 gcagcatttc caaagggctg ggcccgcgct tagccttacc ttctgccgcc gacccctcca
      661 taacgacgct gtgcccgcct tccgtcgtgc cgacaaaaca catcccgtct atccattttg
      721 atgtaacctg catggtgtca ttcctgaaaa tagcgttaaa accgctttgc atatggcgtt
      781 attgtaaaca atttcaagcg gcttatgcag aaatatggac aaaacggcaa aaaaacactt
      841 gaaaaccgat ttacggtttg gctgcctggc cgttgatctg caccgatttg agtttcagcg
      901 tataggtttt gccgtcgtcg gtatagccga tttgtgccgg aatattgttc agggacggtg
      961 cgaagaaata cattaccgca tcgtcgccgc gccgcacccg atatttgacg acttcggttt
     1021 ccacgccgcc tatgctgtat tttcctgtac ccgccttatt caaaccgccg acggaataaa
     1081 gttttttgcc gttggtgatt ttcagccccg gggggagttt cgcgtcattt gccgccaact
     1141 gccaggcaag cgtgaacaaa tccatagcct tggggctttg ctcggttttg ctctcgcccg
     1201 ctttgccgta agttacgctg ccgtcggcga atttggcttc cgcatacagt ttgcccctgc
     1261 gtatgtctct atagtaggta gggtgcaggg tattgccgac aaccgtaccg ccggactcga
     1321 aacggatatt gtatagcggc actttaatcg tcgaaacgat tttgtaagca ttgccgctgc
     1381 gttcaaatgt catcgtggcg ggaatgccgt agctgccgga atagtgcagc acggcggatt
     1441 ggggcagccc tgccgcatac gcgcacggca gggcggcgga caaaatggcg gcggaaaata
     1501 tatttttaaa agtcttcatc atttgctccc gcccggttta cgccgtcaga aaacgggcgg
     1561 catcggcgtt ttccgaattt ctgacgcggt ttccctcaat aatcaggcgg ccggcggcaa
     1621 aatcggcaac ggctttcgga taaagtttat gctcgacagc caaaacccgt gcggcaatat
     1681 cgtctgccgt atcgccgtcg agtatcggca caaccccttg cgatacaatc gggccgcaat
     1741 ccagttcggc agtaacgaaa tggatggtgc agccggcaac gcggcagccc gcctccaaag
     1801 cgcgttcgtg cgtatgaagt ccggtaaacg agggaaggat ggacgggtga atgttcatca
     1861 gcctgccttc gtaacgggcg caaaactcgg gggtcagaat ccgcataaaa cctgccaaaa
     1921 ccaccaagtc gggttgatat gcgtcgattt tctccatcat ggcggtatcg aaggcaagcc
     1981 gggatgtaaa gtttttatga ttcaggctat cggtcgggat gccgcgttcg gccgcccatt
     2041 gcaaaccggc agccgtttcg ctgttgctca acacggcggc aatgcggacg ttgtgaatgg
     2101 cggcattgac gattgcctgc atattgctgc cgcgtccaga aatcaggatg acgatgtttt
     2161 tcataatggt gcgcttttga aagggatgcc gtctgaaccg ctgtttggtg gtttcagacg
     2221 gcatttgccg taaaaatgcc cgaaaacctg tttcgggcat ggattcggac ttaatttact
     2281 tttttgatgt cgacttgagc cggctgcttg gcgggcgcgt tttcgggtgc gccgattttg
     2341 accagtttca catcaaatac caaagtggcg ttcggaccga ttttgtcgcc cgcaccctgt
     2401 tcgcggtagg caaggttgga cgggatgtag aacgtggctt cgccgccttc tttcagaagc
     2461 tgtacgcctt cggtccaacc cggaatcact tggctcaaag ggaaggtgac cgggccgccg
     2521 ttggctttgc tgctgtcgaa taccgtaccg tcaatcaggc ggccttcgta ttccacggta
     2581 acgatgtcgt ctttggtcgg ctgtttgcct tcgccctgtt tggtgatttt gtattgcagg
     2641 ccggaagcag tggtcttcac gccgtctttg gcggcatttt ctttcagaaa ggcttcgcct
     2701 ttttctttat tggccttcgc gtccgccttg tgtttttcta cggctttagc ctgttgttcc
     2761 tgaaggaatt tcatcatgac ttcctgagcc tgctcttcgg tcattttgat ttctttgccg
     2821 tcatacactg cctgcatggc ttcggtaaag actttcaaat cgatttccgc gccctgttcc
     2881 ttcatttgct tcagggagcg tccgatgtcc acgcccatcg catagcttgc ctgctgcatc
     2941 gtgctgccga tcgaagaggt gtcgccctgc gcggaagaag cggcggcagg ttcggatgca
     3001 gatgcggggg cggcttcttt tttgccgcag gcggaaagtg ccaaagcggc ggaaagggtc
     3061 agtgcgctga ttttgaaaat ggtgttcatg atggatcttc gctgtcgata aggtcggaaa
     3121 aacgggatta tagccgagtt tgaatgtttc aacacacagg atgacacata aagcgtcaat
     3181 cgtgtgttgc cctgttttgg aaaggattga accttccaaa ataagttttg attctaccgc
     3241 cccgagggac agatgtccaa gtggcggggt tcaaccgata aggaaatttt aatcaaatag
     3301 aatcaagcct gtttaaattt tgtaaatgcg gcatttcaga cggcatttta tgccttgccc
     3361 tccatgccgt gatgttcgat ggcaaaaccg cttcggcggt aggcggtaaa gcgttcgcgc
     3421 gcgtcggcga gatcttccaa gctgttgccg acgatttcca aaacgcgcgc gggcaagacc
     3481 ggagcggtgt tccaaaaacc gtcggacagg ttcaaaacag tcatgccttc gggaattcgg
     3541 ggcggattgc cgccgcaagc aagcaggacg gatgtttcag acggcatggc ttcttccatt
     3601 tcccaaattt cgtgcggaat aaaactctcg gcctcgtatt gccaaagcat tttgtccaat
     3661 tcctgaagct gcccgaacga atcggaccac accagtatcc tgccgccgtc ccgaatcgca
     3721 cgggcaatca ggcggcaggt gaaaatcgga acttgggcaa cgtgcgtgta aaaggtggct
     3781 ttcggcatat tgtttgagca tttggcagga cgatgccgtc tgaaaggctt cagacggcat
     3841 tgtggcaaaa ttaaagattc cgcagatagt tcagcagcaa gggaacggga cggccggtcg
     3901 cacctttttc cgcaccggat ttccacgccg tacccgcgat gtcaaggtgt gcccatggat
     3961 agtcttcggt aaagtaggat aggaatgttg cggcggtaat cgtgcccgcg ccgggcgtgc
     4021 cgatgtttgg aatgtcggca aagttggatt tgagttggtc tttgtaggtc tcaaagagcg
     4081 gcagttgcca cgctttgtcg tccacgttgt aggaagcggc aagcaggctg tcgaccaaat
     4141 cttgattgtt gcccatcacg ccgctgacat cgtgccccaa ggcaacaata cacgcaccgg
     4201 tcagggtggc gacgtcgatg acggctttgg gtttgaactg ttcggcgtaa gtgagcgcgt
     4261 cgcacaaaat cagacggcct tcagcatcgg tgttcaacac ttcgatggtc aggcctttca
     4321 tgctttttac gacgtcgccc ggtttgttgg ccgcgccgga aggcatattt tcacaagtgg
     4381 cgacgatggc aatcaggttg atcggcagtt gcagtttgac ggcggcgcag aaggtgctga
     4441 tgacggttgc cgcgccgcac atatcaaact tcatttcgtc catgttcaga ccgggtttga
     4501 gggaaatgcc gccggtgtcg aaggtaatgc ctttgccgac caagacgacg ggggcggctt
     4561 ctttgtcggc tgcaccgaaa tagctcagtt cgaccaaata gggggcttcc gcgctgcctt
     4621 tggcgaccga ccaaaacgaa cccatgtttt ctttgatgta gtctttttcg atgattttgg
     4681 cgtgcgcgcc cagtttttcg gcttcggctt tggcggtgcg cgctaaaaat tcgggcgtgc
     4741 attcgttggg cgcggcgttg cccaagtcgc ggcagaggct ttgtccgtaa acttgcgctt
     4801 cggcgacgcg caaggcttct ttaacgacgg cttcgtgtgc gctgtggaac acggcggttt
     4861 caaatttggc gggcttggct tcttttttgt agcggtcgaa acggtaggcg gcgttgccga
     4921 acgcaatcgc aaacgcttcg gctacggcgg cggcttgcgc ttcatcgaag gcatggatgt
     4981 ccacattgac cgtttcctga ttttgcgccc atttggcggc ttcggcggcg gccttgttca
     5041 atgcggcgcg gccggtgctt ttcagacagc atacggcaac agcctgcaaa ccgttgcctg
     5101 tcgggatttt tgtgtcggca aaattttgac cttcttcaag cgaagacaaa agggcaaggg
     5161 cggtcggatg gtttaattgc gcctcttcgg tgcagacaaa taactgtgcg cctgcctgct
     5221 gttcctgcaa gatttcggtt tttgtgctaa attccacgtt tattctcctg attgagacgg
     5281 ttgtcggtag ttttcggacg gcctttcgct caaaagaccg tctgaagacg gctggcacga
     5341 ttgtacccca tttgaagcac cgtctgaaac cttgcgcgga caatccgcct gcgccgaacc
     5401 gcttaccgcc ccctgaccgc gattctatga tttatcaaag aaacctcatc aaagaactct
     5461 cttttaccgc cgtcggcatt ttcgtcgtcc tcttggcggt attggtctcc acgcaggcaa
     5521 tcaacctgct cggccgtgcc gccgacgggc gtgtcgccat cgatgccgtg ttggcattgg
     5581 tcggcttctg ggtcatcggt atgacgccgc ttttgctggt gttgaccgca tttatcagta
     5641 cattgaccgt gttgacccgc tactggcgcg acagcgaaat gtcggtctgg ctatcctgcg
     5701 gattggcatt gaaacaatgg atacgcccgg tgatgcagtt tgccgtgccg tttgccgttt
     5761 tggttgccgt catgcagctt tgggtgatac cgtgggcaga gctacgcagc cgcgaatacg
     5821 ctgaaatcct gaagcagaag caggaattgt ctttggtgga ggcaggcggg ttcaacagtt
     5881 tgggcaagcg caacggcagg gtttattttg tcgaaacctt cgataccgaa tccggcatca
     5941 tgaaaaacct gttcctgcgc gaacaggaca aaaacggcgg cgacaacatc atcttcgcca
     6001 aagaaggtaa cttctcgctg aacgacaaca aacgcacgct cgaattgcgc cacggctacc
     6061 gttacagcgg tacgcctgga cgcgccgact acaatcaggt ttccttccaa aaactcaacc
     6121 tgattatcag caccacgcca aaactcatcg accccgtttc ccaccgccgt accattccga
     6181 ccgcccaact gattggcagc agcaacccgc aacatcaggc ggaattgatg tggcgcatct
     6241 cgctgaccgt cagcgtcctc ctactctgcc tgcttgccgt gccgctttcc tatttcaacc
     6301 cgcgcagcgg acatacctac aatatcttga ttgccatcgg tttgttttta atttaccaaa
     6361 acgggctgac cctgcttttt gaagccgtgg aagacggcaa aatccatttt tggctcggac
     6421 tgctgcctat gcacatcatc atgttcgtca tcgcaatcgt acttctgcgc gtccgcagca
     6481 tgcccagcca gcccttctgg caggcggttg gcaaaagtct gacattgaaa ggcggaaaat
     6541 gaacctgatt tcacgttaca tcatccgtca aatggcggtt atggcggttt acgcgctcct
     6601 tgccttcctc gctttgtaca gcttttttga aatcctgtac gaaaccggca acctcggcaa
     6661 aggcagttac ggcatatggg aaatgctggg ctacaccgcc ctcaaaatgc ccgcccgcgc
     6721 ctacgaactg attcccctcg ccgtccttat cggcggactg gtctccctca gccagcttgc
     6781 cgccggcagc gaactgaccg tcatcaaagc cagcggcatg agcaccaaaa agctgctgtt
     6841 gattctgtcg cagttcggtt ttatttttgc tattgccacc gtcgcgctcg gcgaatgggt
     6901 tgcgcccaca ctgagccaaa aagccgaaaa catcaaagcc gccgccatca acggcaaaat
     6961 cagcaccggc aataccggcc tttggctgaa agaaaaaaac agcattatca atgtgcgcga
     7021 aatgttgccc gaccatacgc ttttgggcat caaaatttgg gcgcgcaacg ataaaaacga
     7081 attggcagag gcagtggaag ccgattccgc cgttttgaac agcgacggca gttggcagtt
     7141 gaaaaacatc cgccgcagca cgcttggcga agacaaagtc gaggtctcta ttgcggctga
     7201 agaaaactgg ccgatttccg tcaaacgcaa cctgatggac gtattgctcg tcaaacccga
     7261 ccaaatgtcc gtcggcgaac tgaccaccta catccgccac ctccaaaaca acagccaaaa
     7321 cacccgaatc tacgccatcg catggtggcg caaattggtt taccccgccg cagcctgggt
     7381 gatggcgctc gtcgcctttg cctttacccc gcaaaccacc cgccacggca atatgggctt
     7441 aaaactcttc ggcggcatct gtctcggatt gctgttccac cttgccggac ggctcttcgg
     7501 gtttaccagc caactctacg gcatcccgcc cttcctcgcc ggcgcactac ctaccatagc
     7561 cttcgccttg ctcgccgttt ggctgatacg caaacaggaa aaacgttgaa ccaatgccgt
     7621 ctgaacctct cttcagacgg catttgtttt cattgacaca ttcccacaga cagatagccg
     7681 ttccctatta cattacctgt cataacagtt ccatttttgt taaaactagt ctatgatagc
     7741 ggtacaaata ttgtttacaa tatttaacgc aaatcatttg caacccgaca aaagaaaaac
     7801 agaaaaagga acaaagagat gttagaagcc tatcgtaaag ccgccgccga gcgcgccgcc
     7861 ctcggcattc ccgccctccc tttgaacgcg cagcaaaccg ccgatttggt tgagctgctg
     7921 aaaagcccgc ccgcaggcga aggcgagttc ttggtcgaac tgcttgccca ccgtgttccg
     7981 cccggtgtgg acgatgccgc caaagtcaaa gcctcattcc tggctgccgt tgccgaaggc
     8041 agcgcgtcca gcccgctgat ctcccccgaa tatgcgaccg aactcttagg tacaatgctc
     8101 ggcggttaca atattcacgc cttaatcgaa ctcttggacg acgacaaact cgcgtccatt
     8161 gctgccaaag gcttgaaaca tacgcttctg atgttcgatt ccttccacga cgttcaagaa
     8221 aaagccgaaa aaggcaacaa atacgcgcaa gaagttttgc aatcttgggc agatgccgaa
     8281 tggttcgcct cacgcgccaa agttcccgaa aaaatcaccg ttaccgtttt caaagttgac
     8341 ggcgaaacca atacagacga cctctccccc gcgcccgacg cgtggagtcg tcccgatatt
     8401 ccgctgcacg cgctggccat gctgaaaaac ccgcgcgacg gcatcacgcc cgacaaaccg
     8461 ggcgaagtcg gtccgattaa attgttggaa gaactcaaag ccaaaggcca tccggttgct
     8521 tacgtcggcg acgtggtcgg tactggttct tcacgcaaat ccgcgaccaa ctccgtcatt
     8581 tggcataccg gcgaagacat tccgttcgtg ccgaacaaac gcttcggcgg cgtatgtttg
     8641 ggcggcaaaa tcgcgccgat tttcttcaat acccaagaag attccggcgc gctgccgatt
     8701 gaagtcgatg tatctgctct aaaaatgggc gatgtcgtcg atatcctgcc ttatgaaggc
     8761 aaaatcgtga aaaacggcga gactgttgcc gagtttgaat tgaaatcaca agtattgctg
     8821 gacgaagtgc aagccggcgg ccgtatcaac ctgattatcg gccgaggtct gaccgccaaa
     8881 gcgcgcgaag ccctgaaact gcctgcctct actgcattcc gcctgccgca agcgcctgcc
     8941 gaaagcaaag ccggtttcac cttggcgcaa aaaatggtcg gccgcgcctg cggtctgccc
     9001 gaaggacaag gcgtgcgccc gggtacttac tgcgaaccgc gtatgacgac ggtcggctcg
     9061 caagacacga ccggcccgat gacccgcgac gagttgaaag acttggcttg tttgggcttc
     9121 tccgccgata tggtgatgca gtctttctgc cacaccgccg cctatccgaa acctgtcgat
     9181 gtaaaaaccc ataaagaact gcccgccttt atttccaccc gtggcggcgt gtcactgcgt
     9241 ccgggcgacg gcgtcatcca ctcgtggctc aaccgcctgc tgctgcccga taccgtcggc
     9301 accggcggcg acagccatac ccgtttcccc atcggtattt ccttccccgc cggctccggc
     9361 ttggttgcct ttgccgccgc aacgggcgta atgccgctcg atatgcccga gtctgtattg
     9421 gtacgcttca gcggcaagct gcaaccgggc gtaaccctgc gcgatttggt gaacgccatc
     9481 ccgctgtacg caatcaaaca aggtttgctg accgttgcca aagccggtaa gaaaaacatc
     9541 ttctccggcc gcatcctcga aatcgaaggc ctgcctgatt tgaaagtgga acaagccttt
     9601 gaattgaccg acgcatccgc cgaacgctcc gccgccggct gtaccgtgaa gctcaacaaa
     9661 gagccgatta tcgagtacat gaaatccaac gtcgtgttga tgaaaaacat gattgccaac
     9721 ggctatcaag acccgcgcac tttggaacgc cgcatcaaag ctatggaaaa atggctggca
     9781 aatcccgagt tgctcgaagc ggataaagat gccgaatacg ccgccgtgat tgaaatcaac
     9841 atggacgaca tcaaagagcc gattatcgcc tgcccgaacg acccggacga cgtgtgcttc
     9901 atgtccgaac gctccggcac caaaatcgac gaagtattca tcggttcgtg tatgaccaac
     9961 atcggccact tccgcgccgc ctccaaactt ttggaaggca aggcagacac ccccgtccgc
    10021 ctgtggattg cgccgccgac caaaatggac gcgaaacaat tgtccgacga aggacactac
    10081 ggcgtactcg gacgtgccgg cgcgcgtatg gaaatgccgg gttgctcctt atgtatgggt
    10141 aatcaggcgc aagtacgcga aggtgcgacc gttatgtcca cctccacccg caacttcccg
    10201 aaccgtttgg gtaaaaacac ctttgtttac ctcggttcgg cggaattggc agcgatttgc
    10261 tccaaactgg gtaaaatccc gaccgttgaa gaatatcaag ccaatatcgg catcatcaac
    10321 gaacagggcg ataaaatcta ccgctatatg aacttcaacg aaatcgacag ctacaacgaa
    10381 gtagccgaga ccgtgaacgt ttaatccccg tcatccgtat gaagtaaggg attgaccgca
    10441 atgccgtctg aacaaccttc agacggcatt gcaacattcc gctaaccctt ctttccgcaa
    10501 acgctgcaaa tacggcgttc acgcccccac ataaaggaaa cgacagtgaa cctgaaaaac
    10561 cgccattttc tgaaactttt agacttcacg ccggaagaaa tcaccgccta cctcgacctt
    10621 gccgccgaat tgaaagccgc caaaaaagca gggcgcgaga ttcagcggat gaaagggaaa
    10681 aacatcgccc tgatttttga aaaaacctct actcggacgc gctgcgcgtt tgaagtcgcc
    10741 gcgcgcgatc aaggcgcggg agtgacttat ttagagccgt ccgccagcca aatcgggcat
    10801 aaggaaagca tcaaagacac cgcccgcgtg ttgggcagga tgtacgatgc catcgaatat
    10861 cgcggtttcg gtcaggaagt tgttgaagaa ttggcgaaat acgcgggcgt acccgtgttc
    10921 aacgggctga ccaacgagtt ccatcccaca caaatgcttg ccgacgcact gactatgcgc
    10981 gaacacagcg gcaaaccttt gaaccaaacc gcgtttgcct acgtcggcga cgcgcgttac
    11041 aacatgggca attccctgct gattttaggg gcaaaattgg ggatggacgt gcgtatcggc
    11101 gcaccgcaaa gcctgtggcc gtctgaaggc attattgccg ccgcacacgc cgccgccaaa
    11161 gaaaccggcg caaaaattac cctgaccgaa aacgcgcatg aagccgtgaa gaatgttgat
    11221 tttattcata ccgatgtgtg ggtcagcatg ggcgagccga aagaagtctg gcaggaacgc
    11281 atcgatttgc tgaaagatta ccgcgttacg cccgaactga tggcggcatc gggcaatccg
    11341 caagtcaaat tcatgcactg cctgcccgcc ttccacaacc gcgaaaccaa agtcggcgaa
    11401 tggatttacg aaaccttcgg gctgaacggt gtggaagtta cagaagaaat attcgaaagc
    11461 cccgccagca tcgtgttcga tcaggcggaa aaccgtatgc acacgattaa agcggtaatg
    11521 gtcgcggctc tgggcgactg acagaactgt gcctgtttaa attcatccgc aacacagata
    11581 ccgtctgaac acgatgttca gacggtatcc atatatagtg gattaaattt aaaccagtac
    11641 ggcgttgcct cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
    11701 tttttgttaa tccactataa aaaaactgcc tacacgatgt gtaggtagtc ccgtttgaaa
    11761 acaatcagtt tttgtcttgg tcaaccaatt tgttggcagt aatccaaggc atcatggcac
    11821 gcagttgtgc gccgactttt tcaacttggt ggtcggcatt cagacggcgg cgggcagtca
    11881 tagacgcata gttgacatta ccctcttgga taaacatttt tgcgtattcg ccggtttgaa
    11941 tgcgtttcag ggcattgcgc atggcttctt tgctggaagc attgaccact tcagggccgg
    12001 taacgtattc gccgtactcc gcattgttgg aaatggagta gttcatattg gcaataccgc
    12061 cttcgaaaat caggtcaacg atcagtttca tttcgtgcag acattcgaag taagccattt
    12121 caggcgcgta accggcttcg gtcagggttt caaaacccgc cttgatcaac tcgaccacgc
    12181 cgccgcacaa tacggcttgt tcgccgaaca gatcggtttc ggtttcttcg cggaaagtgg
    12241 tttcaatcac accgcctttg gtgccgccgt tggcagccgc ataagacagg gcgatgtctt
    12301 tggctttgcc ggaattgtct tggtaaacgg caatcagaga aggcacgccg ccgccgcgtt
    12361 tgtattcact gcgtacggta tggcccggac ctttgggggc aaccataatc acgtccaagt
    12421 cggcacgcgg aacgatttgg ttgtagtgca cgttgaagcc gtgtgcaaat gccagcgttg
    12481 cgccttcttt caaattggct gtaacttcgg cgtgatagac ggcaggcatg gtttcgtcag
    12541 gcagcagcag cataacgaca tcggcttctt tggtcgcttc agcaacggtt ttgacgacat
    12601 aaccggctgc ttcggctttt ttccaagaag aaccttggcg cagaccaatc accacgttta
    12661 cacccgaatc tttcaggttg gcggcatggg catgaccttg cgaaccgtaa ccgatgatgg
    12721 caacggtttt gcctttgatt agggacagat cggcatcttt atcgtaatag acttgcattt
    12781 gatttccttt aaggtaaatg gttgtcgaag ccttaaaatg ttgagcggct tcggacgggt
    12841 taaacagagt gtgccgctta atcggcaact tcattcatca atacgatttc caacgcttcg
    12901 gttttgccgt cgacggactg gacgaaggct tggaaatgcg cgctggcgtt atgttcgtca
    12961 atagctgctt gagatttcca attttccacg aaaacaaaac ggttcggttt gccgatttcc
    13021 tgatggagat cgtagctgat gttgccctct tctgcacggc tggctttgac cagttcttta
    13081 aactgtgctg ccagtgtttc tgtgtattcc ggtttgacgg taaccagtgc gacaatttta
    13141 atgttcgaca taaatctctc ctgccgttcg tttttcagac gacattcaaa taccgtgccg
    13201 tctgaaaggt tacggcgtta aattttcaaa atacgctcac cgcgaccgat gccggccgcg
    13261 cctgtgcgta cggtttccaa aatttgggcg cgtccgaccg tttccaaaaa ggaatccagc
    13321 ttgtctgtcg agccggtaat ttcaatcgta tagctgcggt cggttacgtc gatgatgctg
    13381 ccccggtaga tttcggtcaa gcgtaaaaat tcgtcgcggt ctttgccggc ggcacggact
    13441 tttaccaaca tcagttcgcg ttcgacaaaa cggctttcat tcaaatcgac cactttaatc
    13501 acttcaatca atttattgag ttgcttggta atttgttcga tgacctgctc gtcgccgtgg
    13561 gtaacgatgg tcatccgtga cagggttttg tcttcggtcg gcgcaaccgc caaagaatcg
    13621 atattgtaat cgcgtgcaga gaacaaaccg accacgcggc tcatcgcacc tgattcgttt
    13681 tcaatcagaa cagataagat atgtcgcatt tgtctctcct tacgcctttc cgtccgcacg
    13741 catatgcggc ggaagtacca tttcgtccaa acctttgccg ttgccgacca tgggcatcac
    13801 attctgtttc tggtcggtca ggaagtcgat aaacaccagc ctgtcttttt ggttcaatgc
    13861 ttccaacaac gcaccttcca catcagactt cttgtccacg cggataccga tatggccgta
    13921 tgcctcggca agtttgacga aatcgggcaa agaatcgaaa taggtttccg actctcgtcc
    13981 gccgtaatat atttcctgcc actggcgtac cataccgaga taaccgttgt tcagcgtaat
    14041 gacgttaacc ggaatccgat attggaaaca ggtggacagc tcttggatgt tcatctggat
    14101 cgagccgtcg ccggtgatac agaatacgtc ttgatccggg gcggcaagtt ttgcaccaat
    14161 cgcataaggc agacccacgc ccatcgtacc caaaccgccg gaattgagcc attggcgcgg
    14221 acgttcgaag ggataatatt gagccgcaaa catttgatgc tgccctacat ccgatgtgat
    14281 gattgccgaa ttgccggtaa tctcggcaag cttctgaatc acatattggg gcttgataat
    14341 ttcgctgccg ttgtcaaacc acaagcaatc ttgggaacgc cattcctcta tggttttcca
    14401 ccatttgccc aaagcatctt cagacggcac ggactcttgt ttttgccaca gcgcaaccat
    14461 ctcggacaaa acgtttttca cgtcgccgac aatcggaatg tccaccttca cgcgtttggc
    14521 gatgctggaa ggatcgacat cgatatggat aaccttcttc gccttctcga aaaatttgga
    14581 cggtacggaa accacacggt cgtcaaaacg cgcacctacg gcaagaacga catccgcatt
    14641 ctgcatggca aggtttgcct cgtaagtacc gtgcataccg agcataccga ggaattggcg
    14701 gtcgctggaa ggataagcgc ccaagcccat cagcgtaccc gtgcacggag cacccgtcat
    14761 tcggacaaat cgggtcagct cttcagaagc attacccaac accacgccgc cgccaaaata
    14821 gacgactgga cgtttggcag atgccaacat ctgcacggcc tttttaatct gaccgatatg
    14881 cccttgaaca accggttgat acgaacggat aaaaatgtct tcctgaggat agctgaattt
    14941 cgccatcgcc tgcgtaacat ctttcgggac atcaaccacc acaggccccg gtcggccgct
    15001 tgcggcaatt tggaacgcct ttttaatggt ttccgccaac tcattgatat ccgtaaccag
    15061 gaaattgtgt ttgacgcacg gacgggtaat acccaccgta tcaacttctt ggaacgcatc
    15121 cgtaccaatc agggaattgc ctacctgccc gctgatgacc accatcggaa tcgaatccgt
    15181 ataggcagta gcaataccgg tcagtgcatt ggtaacgccc gggccggatg taaccaatgc
    15241 cacgcccacc ttaccgctga cgcgcgcata cgcatctgcc gcgtgtactg ccgcctgctc
    15301 gtgacgggtc agaatgtgct tgaatttatt gagttggaaa agggcatcgt agatttcgat
    15361 aaccgcaccg ccgggataac cgaaaacgta ctcgacacct tcggctttga gactctgcac
    15421 tatgatttgc gcgcctgata actgcataac gacctctttt atacggtttc aaaccaatag
    15481 ggacaaaccg ctttgccaca gcacctgtaa tgcaattcca ccaagcagcg atttagggta
    15541 cgcgcattgg gggaacacgg caacagacgg attatccaat caattggaaa ggaacacaga
    15601 gtttgtgaaa aagagtagaa acgataacgc aaaccgacag ttcaatcaag aaaaatcttt
    15661 catcttttaa tattttttga aagcagagaa attattgatt gattttaaaa gaataaaatc
    15721 aggagtacct tttttgaaag atggaaattg ttgacagttt gtgtaggagg ggcagatgtg
    15781 aaaaaccctt cttcgatatc aagaattgta aaatttacag ggtttcatcc caataaagac
    15841 tcgggatatt gattgaactt gattttattt ttgatatatc aaaaatattc ccaaccatac
    15901 ttcctgaaaa tggctcattg caccggactg tattggacgg cattgacaga accaagaggg
    15961 ctaacaacga cttaatatat tg
//
LOCUS       DAWHRS010000033        15280 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWHRS010000033 DAWHRS010000000
VERSION     DAWHRS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.033"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(79..315)
                     /locus_tag="WIB82_000727"
                     /pseudo
     CDS             complement(79..315)
                     /locus_tag="WIB82_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            511..969
                     /locus_tag="WIB82_000728"
                     /pseudo
     CDS             511..969
                     /locus_tag="WIB82_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            869..1237
                     /locus_tag="WIB82_000729"
     CDS             869..1237
                     /locus_tag="WIB82_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ3842061.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1306..2844
                     /gene="lnt"
                     /locus_tag="WIB82_000730"
     CDS             1306..2844
                     /gene="lnt"
                     /locus_tag="WIB82_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ3842062.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASNNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMDMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3108..3971
                     /gene="rpoH"
                     /locus_tag="WIB82_000731"
     CDS             3108..3971
                     /gene="rpoH"
                     /locus_tag="WIB82_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ3842063.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(4030..4176)
                     /locus_tag="WIB82_000732"
     CDS             complement(4030..4176)
                     /locus_tag="WIB82_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842064.1"
                     /translation="MLIHYINFANLSEKQLMRQCLVHVCFLISAIAMAWTDKIVYGTT
                     HKPH"
     gene            4455..5339
                     /locus_tag="WIB82_000733"
     CDS             4455..5339
                     /locus_tag="WIB82_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ3842065.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKNLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSEFFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIITEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5423..5938)
                     /locus_tag="WIB82_000734"
     CDS             complement(5423..5938)
                     /locus_tag="WIB82_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ3842066.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(6014..6433)
                     /locus_tag="WIB82_000735"
     CDS             complement(6014..6433)
                     /locus_tag="WIB82_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842067.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(6554..7552)
                     /locus_tag="WIB82_000736"
     CDS             complement(6554..7552)
                     /locus_tag="WIB82_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ3842068.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGYTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLREEGGEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWAAFKQLAVSLAK"
     gene            complement(7554..8033)
                     /locus_tag="WIB82_000737"
     CDS             complement(7554..8033)
                     /locus_tag="WIB82_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842069.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALR
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(8085..8864)
                     /gene="pgeF"
                     /locus_tag="WIB82_000738"
     CDS             complement(8085..8864)
                     /gene="pgeF"
                     /locus_tag="WIB82_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ3842070.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVDDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKAACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            9031..9144
                     /locus_tag="WIB82_000739"
                     /pseudo
     CDS             9031..9144
                     /locus_tag="WIB82_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9423..10370
                     /locus_tag="WIB82_000740"
     CDS             9423..10370
                     /locus_tag="WIB82_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ3842071.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(10522..11646)
                     /gene="rluD"
                     /locus_tag="WIB82_000741"
     CDS             complement(10522..11646)
                     /gene="rluD"
                     /locus_tag="WIB82_000741"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ3842072.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            11645..12448
                     /locus_tag="WIB82_000742"
     CDS             11645..12448
                     /locus_tag="WIB82_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ3842073.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(12506..14725)
                     /locus_tag="WIB82_000743"
     CDS             complement(12506..14725)
                     /locus_tag="WIB82_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ3842074.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVADGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         3692 a         4596 c         3741 g         3251 t
ORIGIN      
        1 cgaccttgcc gtcgggcagg atgtaccacg ccaaatcccc gcctgaagca cggtgtcgga
       61 ctgcatttct attcggctat acgccttttc acgcaggctg taattgaatt tccacaaacc
      121 gccgaacact gccgacggac gcgcgaccag catcaggaac gccgcaatcg ccaaggcttc
      181 cgccgccctg tccaacacgt tggcaggaga aaccagcagc aaaggcattc ccaaacgtgc
      241 ggacaaagtg gtcgaaacca cgctcagaaa caacagtgcg ccacccggca ggaacagact
      301 gcccatccaa tccacgctaa cgattctccc ttaaaattca tgaaattaaa atcgaaagac
      361 tgtaacacgg tattttcttt tcacgaaact ttacttcacg tccgccacag cgttcagacg
      421 gcattttcct gtttcatgcc gcctgaaacc gcccaaatcc gcgttctcct atataatgtc
      481 ggactttgcg ccgcatttca ggattcttta atggcatttg cctcactatt taccctgccg
      541 gacgacatca ccgccgtttt ggacgatgtt gccctgatga ccaaagccgc cgcaaagaaa
      601 accgccggcg tggtcggcga cgatttggca cccaatgcca accgggttac gggcgtatcg
      661 gcagaacgcg agttgccgat tgtttggtcg atggcgaaag gttctttttc caacaagccg
      721 gttttggtgc ctgcggccct gctgctttcc gccttcctgc cgcaactcat tacgccgctt
      781 ttgacggcag gcggcattta tctgtgcttt gaaggcgtag aaaagctgct gcacaagttt
      841 ctgcaccgac atgacgcgca tgctcgtaat ggcggcaatc ggcatcggca tgactgtttt
      901 ggtgtacgga atcgttgccg tcatcgtcaa actcgacgac ttaggcatgc tgctgatgcg
      961 ccgcccacaa accttttcgc gcagtttggg gcaaatgctg actgccttta tgccttgctt
     1021 tatgcgcggt ttgagcgtgg tcggcacgct tgccatgttt cttatcggcg gcgtactggt
     1081 cgcccacaac ctcggccttc tgcacgattt cctgcacgcg caacactggg acgctggctg
     1141 ggcggagtac ttcgccaacc tcgttgtcgg gctgctttcc ggttcgattg cctgcgcccc
     1201 cgccttgccg ctgatgaatc gtttcggcag gcattgattc ctttttcaaa taccgatgcc
     1261 gtttgaaaga tgttcagacg gtatcttccg aacagacaga tgaatatggt ttccaaactg
     1321 gacaaatact ggcagcaccc cgccctctac tggcctttgc tcatcctttt tgccgccgcc
     1381 acccccttta ccttcgcacc ctactaccac ttttggctga tgcccttgat tttcggtgcc
     1441 ttcgtccgcc tcatcgaact gcgtccgcgt tttgctgtct cttccgccta cctgttcggc
     1501 ctgaccgcat acacgacaca gttctactgg atacacaccg ccctgcacga cgtttccggc
     1561 ctgcccgacc tctatgccgt accgctgacc ttcctactcc ccgcctacct tgccctttat
     1621 ccggcactgt gtttctggct gtggaaaaaa tttaccctgc ctcggggcat aaaaatcggt
     1681 ttggtactgc ccatcctgtg gacgctgacc gagtttgccc gcgaacgttt cctgaccgga
     1741 ttcggctggg gcgcaatcgg ctactcccaa atcaccccgg acagcccgct cgccggcttt
     1801 gccccattgg gcggcatcca catggttaca ctggcaaccg cctttctcgg tgtctggctg
     1861 gttttggcga gtaataacac cgcacgttcg ggcaaacgcc tgcttccgat tatcctgatt
     1921 gccgccctgc ttgccgcagg ctacaccgcc cgacaaaccg acttcacccg ccccgacggc
     1981 agccgcagca ccgtcgccct gcttcaaggc aacatcgacc aaaccctcaa atggcgtgaa
     2041 gaccaagtta tcccgaccat acagaaatat tacgaacaag tcggcaaaac caccgccgac
     2101 atcgtcatcc tgcccgaaac cgccatcccc gttatgcgcc aaaacctgcc ggaaaacata
     2161 ctggcgaaat ttgccgaaca ggcgcaaaac aacggcagcg cgctcgccgt cggcatcagc
     2221 caatacactt cggacggcaa cggttacgaa aacgccgtca tcaacctgac cggttatcag
     2281 gaaaacaatc aggacggtat cccctactac gccaaaaacc acctcgtccc cttcggcgaa
     2341 tacaaaccgc tgcctttcct gaccacgccg ctttacaaaa tgatggatat gcccctttcc
     2401 gacttccgca aaggcggcgg caagcaatcc gccctgctga tgaaaaacca aaaaatcgcc
     2461 ttcaacatct gttacgaaga cggattcggc gacgaactga ttgccgccgc aaaagatgcc
     2521 accctgcttg ccaatgccag caatatggcg tggtacggaa aatccaacgc catgtaccag
     2581 cacctccaac aatcgcaggc gcgggctatg gaactcggac gctatatggt ccgcgccacc
     2641 aacaccggcg caaccgccat catctccccc aaaggcaaca tcatcgccca agcccaaccc
     2701 gatacggaaa ccgtattgga aggacacatc aaaggctatg tcggcgaaac gccctatatg
     2761 aaaaccggca gttcatggtg gttgatgggc atattgaccc tagccgcact gattcttttc
     2821 atcttccgaa acaaagaaca ctgatacctg cacagcccgt ccagacgggc tgtctttttt
     2881 caaaccggct gtttacatac aactacgtat gtaatataat tccgaaatcg gattgagcca
     2941 tcaggtttac aaaatcaatc aaacctgcct tacaaaattt tcctgtcttt caaaccttag
     3001 cgcatatctc ctgccaaaca tacaaaatac aagccaagca gtgatatact gcatcccaac
     3061 acagcaaacc gccgatacgc agtttcagcc gaaaggaaat gccccaaatg aataacgctt
     3121 tcgcattacc cgccatccaa agcggcaacg gcagcctcga acaatacatc cacaccgtca
     3181 acagcatccc gatgctgtcc caggaagaag aaacccgcct cgccgaacgc cggataaagg
     3241 gcgacctcaa cgccgccaaa caactcatcc tgtcccacct gcgcgtcgtc gtttccatcg
     3301 cgcgcggcta tgacggctac gggctgaatc aggcagacct gattcaggaa ggcaatatcg
     3361 gactgatgaa ggcggtcaaa cgctacgagc caggtcgagg tgcacgcctg ttttcatttg
     3421 ccgtgcattg gattaaagcg gaaattcacg aattcatcct gaggaactgg cgccttgtcc
     3481 gcgttgccac caccaaaccg caacgcaagc tgtttttcaa tttacgcagt atgcgtaaaa
     3541 acctcaacgc cctgtctccc aaagaggcac aagacatcgc cgacgatttg ggtgtcaaac
     3601 tttccgaagt tctggaaatg gaacagcgca tgacgggaca cgacatcgcc atcatggcag
     3661 acaacagcga cgacgaggac agtttcgccc ccatcgactg gcttgccgac cacgattccg
     3721 aaccgagccg acaactatcg aaacaggcgc attacgccct gcaaacagaa ggtttacaaa
     3781 acgctttggc gcaactggat gacaggagcc gccgtattgt ggaaagccgc tggcttcaag
     3841 atgacggcgg tctgacgctg caccagcttg ctgcagaata cggcgtctct gccgagcgca
     3901 tccgccagat tgaagcgaaa gccatgcaga aactgcgtgg tttcctgacc gaagaagccg
     3961 aagcggtttg aaatatacgc aataaaatac ccgacaaatt gttttgtcgg gtattttatt
     4021 tggtaaacat caatgcggtt tgtgcgtcgt gccgtacacg attttatccg tccaagccat
     4081 cgcaatcgcc gaaatcaaaa agcagacatg caccagacac tgccgcatca gctgcttttc
     4141 cgacagattg gcgaaattga tatagtggat taacaaaaat caggacaagg cggcgaagcc
     4201 gcagacagta caaatcgtac ggaaccgatt cgcttggtgc ttcagcacct tagagaatcg
     4261 ttctctttga gctaaggcga ggcaacgccg tactggtttt ggttaatcca ctatacattt
     4321 cccttccttg cagcaacaaa agcgcgtgat tccaatctgc cgctgcaaaa tcttcaattt
     4381 ttcacacccg aaaacgcttg ccgccataca gatgtgatat attgattcca ttttaacggg
     4441 cttcggagaa cgacatgagc agcggaaata tccacatcca cagcatgacc ggatttgcca
     4501 acgcggcggc agagtgcggc agcaaacgca tcaatctcga catccgcgcc gtcaaccacc
     4561 gctttttgga cattcaaatc aggatgccgg atgatttgcg ctatttggaa agcggtatcc
     4621 gcgagaaaat ttcatcccat atcgcgcgcg gcaaagtcga atgcaaaatc caaattcaag
     4681 atacggaaaa cagcagacag tccttggaac tcaactcgga tttggtgaac cagcttgccg
     4741 aaatcaacaa aaacctccgc aagcatcacg atttggcaaa actgggcgtt gccgacatcc
     4801 tgcgtttccc cggcgtattg gcaagccaaa gggaaaatac ggaggaattg gcaaaaagca
     4861 ttaccgaact gaccgaaaaa gcgttaaaag actttactgc cgcacgccgg cgcgaaggga
     4921 aaaaattggg cgagcacctg ttacaacgcc tcgaaagcat ggaagaaatc atagatgcat
     4981 tgagcgaatt ctttcctacc ctgctggaaa cacataaaga aaaaatccgc gcccgcctcg
     5041 tcgaagcagt cggcgacatc aataatgaca ggctgcaaca ggaatttgcc ctctttattc
     5101 aaaaatccga catcgacgaa gaattcagcc gcctacgcac ccacattgcc gaagtgcgtc
     5161 gtatcattac cgaacacaaa ggcagcagcg gcaaacggct ggacttcctg atgcaggaat
     5221 taaaccgcga agccaacact ttgggcagca aatccattgc cgccgaatgc acccaagcct
     5281 cggtcgagct gaaagtcctg atcgaacaga tgcgcgaaca ggtgcagaat atcgaataat
     5341 cggtttcccc gataagcaaa tgccgtctga aagccctttc agacggcatt ttttcaggtt
     5401 gaaacctttg cggtataaag gcttaaggct ttcctttgac cgccaccaat cttgttcctg
     5461 caagaaaatc atacagaaac tgccgatcgg gattcagcag tgcgaacccc cacggtaaaa
     5521 tcagccaaat caatgccgcg ccggccgcgc ccttgggcgg aatgccgagg aagtggcgta
     5581 atccggcata agcaagcata ggaatgaaga cgataaatat gcacgcccaa ataaagcgca
     5641 ggcgcagcag gtgcaaagac ggctgtatgc cgttaaggtc gcacaagccg attttccatg
     5701 tcctcatcgc caaggtctgc ccctgaccgt gccagttggc gcgaaaataa agccaccatg
     5761 atcccattat caggatactc gttaccaatg cagaaaccgc gatagaaacg ggattcagaa
     5821 aaatggcggc aataccggca aacaatgctg ccaaacaggt tgccgcaccg accagcagca
     5881 tttcgtacag catggcggca aagcggcgtt tgagcggggc ggttttcagt gtcatcatct
     5941 tttcaatcag aatatgccgt ctgaaacgcc aacaggcttc agacggcatt tatgcaaaga
     6001 tgccttgcgg cggttaacgg tgcggacgtt tctgaccgcg cgactgtttt gccgccattt
     6061 gattgatttc ctgacgtgtc gggcgttttt tgaacatagc gtgcgcctcg tccaccgaac
     6121 atttttgcga caggcagatt agggtaatcg catctttctg gtgttgtccg ccgctgtggt
     6181 acatacggcg caggtttgcc tcgttgattc tgcctttttc gggatcgatg tcgaattttt
     6241 tcagacgttc gataaactgg cgttgctgtc ggcgggatgt ccaaacaaag ccgcccgcag
     6301 cggcggcgac gatgacggcg gcaagcagga tggcaagcgg ccaggagtcg acaatcaata
     6361 cgataatgag gcttaaaacg gcggtggtca gtaaaatcag gccgcccaag cgcagtttgg
     6421 tcttattgtc catagggtta atgttccttt attgaggtgg tcgtgccgtc tgaaatgttt
     6481 tcggacggat tggtgaatac ttttcaggat taacggtatt ttacagtatt ttcgtatttt
     6541 ggggattgcc gctttatttc gccagcgaca cggcgagttg tttgaacgcc gcccacgcat
     6601 cgccttcttc cgcacctttg atgattcggt cgatttgggc gcaggttttg agcgcgtcaa
     6661 gcaggcggac gacggaaatc cgcttgaccg caagcggcgc gagcgtctgc ttgtcgcccc
     6721 aaagtctgag actgttgcgg acggattgga tgctctgccc ctgcttcagg gcggcggcaa
     6781 gccggatcag cgtccgcacg tcttcggcaa ccgcccacag aagcagcacc ggttccccgc
     6841 cttcttcccg caatccgtcc aagagcctgc atacgcgcgg gacatcgccc ttcatccacg
     6901 cgcctgccag ttggaacgcg tcgaagcggg cgacgttggc aacggcggtt tgcgcctcat
     6961 cgatattgac ggtataccct ttcggataca gcagcgcgag cttgtcgatt tcctgacgcg
     7021 ccgccaacaa attgccttcc acgcgctcgg caaacagtgc caatgcgtct gcctcgatac
     7081 ccaaaccgat tttgtccagc cgtccgcgta tccattgggg caaggccgcc gcgccgaccg
     7141 gtttggcttc ccacacttcc ccctttgccg ccaatgcggc aaaccatttg gactggagct
     7201 gggttttctc cagtttgggc agcaaaacca gcgttaccgt atcttccggc aggcgggcgg
     7261 caaaatcctg caacgcctcg ccgccggttt tgccgggttt tccgttgggg atatgcagtt
     7321 ccaacagctt caaatcggca aacagacccg cactgcctgc ggtttgcaac agttcgttcc
     7381 aatcgaaggc attgtctgcc gtataaactt cccgattaag gtaaccttgt ttcttcgccg
     7441 ccgccctcaa tgcgtccaat gcctcgatac gcaacagttc ttcctcgccg tggatgacgt
     7501 acaggggttt caaaggcgcg tccgtatcaa tgcgtccgat atgtgccgcc acgtcattcc
     7561 gccttcagaa aggtcaggcg gcggacaatc tgttcggcgg catcctgccg catttccgcc
     7621 cacaatgccg cttcctcttc ctgtttgccc aagatctcgt tgtcggcata agcaaggacg
     7681 cggcggacgg acacggtcat cggtttgccg accggctcgc cgcgtttcaa tacctgcgct
     7741 tcaaccgtca atatcaaaag atattcgttg atgactgccg cacgggtaac ggtgtaggtt
     7801 tccttgtttt gggaaacgct gtctatacgc agggtcatct gcgcgccggc agcatcgtcc
     7861 accctgcccg aagcctgata cagcgctgtt tccaaaggaa accgcaatgc ctgtccgcct
     7921 tcgatgtgcc agctccggta ggtcagcggc ggagaaatgc cgtctgcacc tttcaggtgg
     7981 aaaccgcacg cgcccagcat cagcactgcg gcagtaagaa acagtttgtt catggcttgc
     8041 ctttctcaaa agcacaaagt cggtagatta tatcagcggc gtgttcagac ggcattgccg
     8101 tccagccaaa tcaggctcgc catacgccct gtcgctccgt cgcggcggta ggaaaagaaa
     8161 gtatcccgtt ccaaaaccgt acaatgcgtg ccgccatata cgccgcccac gccttcgcgc
     8221 ttcagaatca ggcgggcgag cgcgtaaagg tcggcaagga atttgccgcc acctatgcct
     8281 tcaaatgcgg cggcggcttc gggcatgggc gtgcagaacg catcaaacac gtcctgtccg
     8341 acttcaaacg catccgcact gatggcgggg ccgagatacg ccatcatttc gacaggcgga
     8401 accttcatcg cggcaatggt gttttgcaat acgccgcccg ccaaaccgcg ccagcccgcg
     8461 tgtgcggcgg caaccgtcgt acccgccctg tcgcaaaaca aaacaggcag gcagtctgcg
     8521 gtcatcacgg cacaggcggc cttgcccgtg tcgtccacgg aagcgtccgc atcgggtgtg
     8581 cctcccaacg cttcggcagc attgacgacg acggtactgt ggatttgatt gaggtaggca
     8641 acgggcaatc cgacctgctg ttgcacgatt tcgcggttgc ggcgcacggc ttcgggattg
     8701 tcgtcgacgt gcgtaccgag gttcaaactc tgatacgcac cttggctcac gccgccgttg
     8761 cgcgtggtaa tcagggtttt cacattggcg ggcgcgggcc aatcggcggt caggaagttt
     8821 ttgcctttcg gggcgagatt tagggtttct gtgatggttt tcattggata ttggaatagg
     8881 tgtgtggcgg aaagggttga ttaactgcct tcggggcaaa tggatgtttg ttttttcgtc
     8941 attcccgcac aggcgggaat tcggagattc gggattgttt tcaaacgttc gggttcggat
     9001 tcttgccggt gcgggaatga tgcccttata tatagtggat taaatttaaa tcaggacaag
     9061 gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc gtactggttt
     9121 aaatttaatc cactatactt tctccgagct ttatatgttt caacagagga cggcacatca
     9181 agcaccgcac tgcgtgttgc ccgaacgaga ctaccctccg attagattct atcgctataa
     9241 acagacgggt ttcaaccgaa aaggaatggg gataaagtcc atttccgaca cctctcgggc
     9301 gatgccgtct gaaaaccaat ctccactttc agacggcatt gtttaggcac ggccattttg
     9361 caaattaagg ttaaggcgtg tatgcttgcc ttcccttaaa aacaaacacg caacgcatta
     9421 ttatgaatat cctcagtaaa atcagcagct ttatcggaaa aacattttcc ctctgggccg
     9481 cgctctttgc cgccgccgct tttttcgcgc ccgacacctt caaatgggcg gggccttata
     9541 ttccttggct gttgggcatt attatgttcg gtatgggttt gacgctcaaa ccttccgact
     9601 tcgatatttt gttcaaacat cccaaagccg tcatcatcgg cgtaatcgca caattcgcca
     9661 ttatgccggc aaccgcctgg ctgctgtcca aactgttgaa cctgcctgcc gaaatcgcgg
     9721 tcggcgtgat tttggtcggc tgctgcccgg gcggtacggc ttccaatgtg atgacctatc
     9781 tggcgcgcgg caatgtggct ttgtcggttg ccgttacgtc tgtttccacc ctgatttccc
     9841 cattgctgac tcccgccatc ttcctgatgc ttgccggcga aatgctggaa atccaagcgg
     9901 ccggtatgtt gatgtccatc gtcaaaatgg ttttgctccc cattgttttg ggtttgattg
     9961 tccataaggt tttgggcagt aaaaccgaaa agctgaccga tgcgctgccg ctggtttccg
    10021 ttgccgccat cgtgctgatt atcggcgcgg ttgttggggc aagcaaaggc aagattatgg
    10081 aaagcggcct gctgattttt gcggttgtcg tactccacaa cggcatcggc tacctgctcg
    10141 gcttctttgc cgccaaatgg accggcctgc cttatgatgc acaaaaaacg ctggccatcg
    10201 aagtcggtat gcaaaactcg ggcctggccg ccgcgcttgc cgccgcacac tttgccgctg
    10261 cgccggtcgt tgccgttccg ggcgcattgt tcagcgtgtg gcacaatatc tccggctcgc
    10321 tgctggcaac ttattgggcg gccaaagccg gtaaacataa aaaaccctaa acgcctttca
    10381 tcataatgcc gtctgaacgg ggcaaacgaa accggcggtt tccgcccgac cccttcagac
    10441 ggcctttgtt tgaaatcggc agggacgatt gtccggctgc tccgattcgg ctgcctgcgt
    10501 tcgccccgcc tttcaagccg cctactccct tacataaacc acttcgacat cgtagtcgtc
    10561 ttcgttccaa tcgtcatcgt cgtccgcgcc gaatttgtcc tgccattcct cttcattgct
    10621 caaagacgaa tccaaaccgg cttcaagacg gaggacagac aacaaatgat atatgtcgtc
    10681 cggaatcggt gcctcaaacg aaacggtttc gccgctttcc ggatgggtga aactcaagcg
    10741 gtaggcgtgc aacgcctgac gcgcgcccaa acttttaacg gcttctttca ccgtgtcgcc
    10801 gcacggatgg cgcgggttgc cgtaaaccgg gtcggcggca agcggatggt tggcctcgcg
    10861 catatggacg cggatttggt gcgtcctgcc cgtttcgagc gagcattcga tatagctgtg
    10921 ggtaagatag cgttccaaca ctttgacgtg ggtaacggct ggtttgccgc cgaatttgac
    10981 gactgccatt ttcaggcggt tgtgcggatc gcgtccgatt tgggtttcga ttttaccgtc
    11041 gaaggggacg atgccgttgg cgacggcgcg gtagatgcgt ttgaccgtgc gctcttgaag
    11101 ctgcctcacg agggaatttt gcgccggcag ggttttggca accaccatca gcccgctggt
    11161 ttccttgtcc aaacggtgta cgatgcccgc gcgcggtact tggctcaatt cgggacagtg
    11221 cgccaacagg ccgttgagca gcgtccccgt ccagttgccc gccgccggat gcaccaccag
    11281 tccggccggt ttgttgacga cgatgacggt atcgtcttcg taaacaatat ccaaagccat
    11341 aggctctgga acaaacgcca gattttcctc actcggacgg acggttacac aaatttgctc
    11401 gccgcctatc attttgtctt tgggttgcga aggtttatcg tttacaataa ccgcgccttc
    11461 tttaatccat gatgtcaggc ggctgcgcga gtagtcgggc agaagtttcg ccaacaccgc
    11521 atccaaccgc ccgccggcaa gctcgagcgg aacggtcaga ccgatacggt tttcagtttc
    11581 ggaagctgac gcaaagtctg aatcgtcgct ataatcggat tcattatcaa aggaagtatt
    11641 ctgcatgaaa aaaattcttt taacggtttc attaggtttg gcactgagtg cctgtgccac
    11701 tcaaggtacg gtcgataaag atgctcagat tacccaagat tggagtgtgg agaagctcta
    11761 tgccgaagcc caggacgaat tgaacagcag caattatacg cgggctgtca agttatacga
    11821 aatcttggaa tcgcgcttcc ccaccagccg ccatgcccgg cagtcgcaac tggataccgc
    11881 atacgcctat tataaagacg atgaaaaaga caaagctctg gcggcaatcg aacgcttccg
    11941 ccgccttcac cctcaacacc cgaatatgga ttacgcgctg tatctgcgcg gcttggtgct
    12001 gttcaacgaa gatcagtctt tcttgaacaa actggcctcg caagactggt ccgaccgcga
    12061 cccgaaagcc aaccgcgaag cgtaccaggc gtttgcggaa ctcgtccaac gcttcccgaa
    12121 cagcaaatac gccgccgatg caaccgcacg catggtcaaa ctggtcgatg cactgggcgg
    12181 caatgaaatg tcggtggcgc gctactacat gaaacgcggt gcatacatcg ccgccgccaa
    12241 ccgcgcccaa aaaatcatcg gcagctacca gaatacgcgt tatgtcgaag aatcgctcgc
    12301 catattggaa cttgcctacc aaaaactcgg caaaccacag cttgccgccg atacgcgccg
    12361 cgtgttggaa accaacttcc cgaaaagccc gtttttgacg cacgcttggc agcccgacga
    12421 tatgccctgg tggcgttact ggcattaagc ccatcttcgt aatgccgtct gaagcccttt
    12481 cagacggcat tttatgtttc aaacctcatt caaacggttt tttctgccaa tagaatttat
    12541 accctctcaa acgttgggcc ttcacgccgc cgcgtcccaa gccgaattgc agtgcgcccg
    12601 acaaatcggt acgcagcagt ttgatgccgt gtgctcggac acggttctgc accgcttcgg
    12661 tcggatgttt gtaggcgttg gcataaccgc ttgaagcaac ggcatattcg ggcgagacgg
    12721 cattgaggaa cacgcccgac gaggacgtat tgctgccgtg atgtcccaac accaaaacct
    12781 ggctgtacag gttgcctcca tacttgccga ccaggctttc ctcacccttc gtatccaaat
    12841 cgcccgttac cagcagtgcc gcaccatcag ccacaacacg caaaacacaa cttttcccat
    12901 tatcatcgat gtttttgcgt tcagacggca tcaaaaactc gaaatccacg ccgtcccatt
    12961 gccaacgctg ttccgcacaa tgccgcgccc cctcataaaa ttccggctgt ccggcataaa
    13021 tcccgccgtt cggtatcttg ccgactgcct gaaaaccgcc gtcgtggtcg ctgtcgtgat
    13081 gcgacaaaac caaataatcc aaatacctga ttcccgacgc attcaaactc ggcacaatcc
    13141 ccatctgcgc cgccgctgcc gttccggtgt caaacaaaag atgatgattt gccgtctgaa
    13201 ccgacaccga caaaccctgc cccgcatccc aaaccgtaac cgcaacctca ttttccggca
    13261 cgccgggtga acggtaaaac acaaaccctg ccaacagcaa caccgcccac ggacgcaaac
    13321 ccaagccgcg cggcagcagc aacagcaaag cggcacacac cgccaacacc aacagcggca
    13381 aaggtgcgga ggcaacggca aactcgggcg acacatcggc aagccacacc aaaaaccgca
    13441 aagtatattc cgccaaaaat gcccccaact gttgcaaagg cgcaaacggc acgaccgaac
    13501 ccagcaacgc cagcggcgtc aatacccaag aaaaccacgg aattgccacc gcattgacca
    13561 aagggctgat taaaggcagc gaagcaaaca gataaccgag caaaaccagc gacaacaccg
    13621 aagccgccca ctgcccgcgc aaagcagttt gccgttttcc ctcgtgcaaa cgccccgagc
    13681 acgcccatat cagggccgcc accaaaccga aagacagcca agtccccaca cccaagaccg
    13741 ccgacgggtc gaacagcagc accgccgcca acgcctgcca ccacgtcgcc cacgccgaca
    13801 atcttcccct gcgccaagcc cacgcaaacg ccgccaacat caaaacgctg cgctgcgtcg
    13861 gcacggaaaa accggcaagc agcgcgtaaa acagcgcgcc tgcacacccc gccgccaaaa
    13921 cccacgcgcg cggccgggca ggaaggcgcg gggaacaggc aagcagccgc ttcgccagcc
    13981 acgcaaacat taccgccacc atcgtaacgt gcaaacccga aatactgacc aaatgcgtca
    14041 gccccaacgg ccggaacgcc tgccacaatt cggggcgcaa tgccgactgt tcgcccacgc
    14101 tcaacgcgcg catcagcccg atgccgtctg aaagcccgcc gtccgcatcc gcctgctgcc
    14161 aattacggct gatgcggctg cgccaaaccg caatccccca accgcttccg ccatgcagca
    14221 aaaccctgtc cgcaccgacc gtccccgcgc cgcctatccc gttggataat gcccacgcct
    14281 cacggttcaa accgcgcagg ttcaattcgc cgacgacggg gtgcacacgt gccgttatcc
    14341 gccatctgct cccgaccgcc cattcgcgcc gtttgtagtc cgacagcagc aaatcaaacg
    14401 tccgaccacc gctgtccaca gcctttgccg caaactgcac acgccgcccg tccgacctcg
    14461 gcatatccgc cacttccacc gtcaaaggca cgcctgaaac ggcttctgcc cgccattgcg
    14521 aagacagtgc cgcttccgtt ctgaatacgc cgtaagccgc gcccgccaac acgcacagca
    14581 tcagaccggc aaacgcaaaa cgccttgcaa acacagcaaa aaccgcaaaa gccgccagcc
    14641 aaaacagcca atgcggcacg accggcagcg caaacgaagc cgccgcgccg acaacccaac
    14701 acggcaaaaa ccaacgacgg aacacgtctc cccaccccag atatttcaaa aataaacgga
    14761 taataaagca aatccgaaaa accgtcataa caagatttga aaatgatata gtggtttaaa
    14821 tttaaatcag gacaaggcga cgaagccgca gacagtacaa gcagtacgac aaggcgaggc
    14881 aacgctgtac tggtttttgt taaaccacta taagaatgca aacgaaaagc ctcagtatta
    14941 tccgagacaa ccgcaaactt tccatcgtca ttcccacgaa cccacatccc gttattacca
    15001 cgaaagcggg aatctaggac tcagggttaa gaaaacctac atcccgtcat tccctcaaaa
    15061 acagaaaacc aaaatcagaa acctaaaatc ccgtcattcc cacgaaagtg ggaatctagg
    15121 tctgtcggtg cggaaactta tcggataaaa cggtttcttt agattccacg ttctagattc
    15181 ccgcctgcgc gggaatgacg attcataagt ttcccgaaat tccaacataa ccgaaacctg
    15241 acagtaaccg tagcaactga accatcattc ccaccacttt
//
LOCUS       DAWHRS010000034        15087 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWHRS010000034 DAWHRS010000000
VERSION     DAWHRS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.034"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(127..1539)
                     /gene="trkA"
                     /locus_tag="WIB82_000744"
     CDS             complement(127..1539)
                     /gene="trkA"
                     /locus_tag="WIB82_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ3842075.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(1637..3160)
                     /locus_tag="WIB82_000745"
     CDS             complement(1637..3160)
                     /locus_tag="WIB82_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ3842076.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMNVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(3467..4273)
                     /locus_tag="WIB82_000746"
     CDS             complement(3467..4273)
                     /locus_tag="WIB82_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ3842077.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDTAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(4325..4576)
                     /locus_tag="WIB82_000747"
     CDS             complement(4325..4576)
                     /locus_tag="WIB82_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842078.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            4676..5437
                     /locus_tag="WIB82_000748"
     CDS             4676..5437
                     /locus_tag="WIB82_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842079.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIESGENADEAESGTNEPAVSENGENDAEETLMPV
                     QLNILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            5582..6751
                     /gene="metZ"
                     /locus_tag="WIB82_000749"
     CDS             5582..6751
                     /gene="metZ"
                     /locus_tag="WIB82_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ3842080.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAMYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            6837..7043
                     /locus_tag="WIB82_000750"
     CDS             6837..7043
                     /locus_tag="WIB82_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ3842081.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(7987..8739)
                     /locus_tag="WIB82_000751"
     CDS             complement(7987..8739)
                     /locus_tag="WIB82_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3842082.1"
                     /translation="MTDILIDNTATEAVRALIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(8843..9310)
                     /locus_tag="WIB82_000752"
     CDS             complement(8843..9310)
                     /locus_tag="WIB82_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ3842083.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            complement(9362..10879)
                     /locus_tag="WIB82_000753"
     CDS             complement(9362..10879)
                     /locus_tag="WIB82_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEZ3842084.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYVWLAIACWLIFFSIIHPHWQWQALKIPSFSAVSDITMIGILTYLF
                     GGINSGFGILILPFVGSSCLLSYGRYPLLYASYAAILLILNALADSNINMYPLILDAK
                     TVTNTFVLVTGSYFVAMIASLSVRYIDRAGKLAHENHAAYRRIRGLNQIVLNRVQEAV
                     VIINVEYQTVLFNKKAKDLLPTLEIGQHTALFDPITVLWDKAPSRTFERNIDTPALTA
                     RIRAVPMNKEQNKLLILYIRPQSEIQAEALSVKLAALGQLTANIAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHLTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            complement(10900..13203)
                     /gene="parC"
                     /locus_tag="WIB82_000754"
     CDS             complement(10900..13203)
                     /gene="parC"
                     /locus_tag="WIB82_000754"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ3842085.1"
                     /translation="MNTQAHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPPHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            13351..14034
                     /locus_tag="WIB82_000755"
     CDS             13351..14034
                     /locus_tag="WIB82_000755"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ3842086.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            complement(14107..15051)
                     /gene="tehA"
                     /locus_tag="WIB82_000756"
     CDS             complement(14107..15051)
                     /gene="tehA"
                     /locus_tag="WIB82_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEZ3842087.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRS"
BASE COUNT         3242 a         3835 c         4437 g         3573 t
ORIGIN      
        1 gctatcccaa ggtctggatg cccgcttacg cgggaatgac ggggagggcg gatgccgtct
       61 gaagcccacc taccattatt tccgcttcca taccgcttgg cggcaatatg tccggaatgg
      121 ggcggtttat ccgaaaaagc ccattttgac ctggatgagt ttttccagtt cgttcaggat
      181 gcgccggcgc gagacgaaaa agatgatgtg gtcgccgtct tggatgacgg tttcggtatg
      241 gtgtcccata atggtttcgc cggttccggc gcggacgacg gcggcaatgt ggcagccttc
      301 gggccatttg atgccgctga tgcgcctgcc gatgatggcg gaagtttttt tgtcgccgtg
      361 tgcgacgact tcgatggctt ccgccgtgcc gcgccggatg gggtggacgg caacgatgtc
      421 gccgcgccgg atgtgggcga gtatcgagcc gatggtgatg aggtgggggg agacgacgat
      481 gtcgattttg ttgccttcga gcaaatcgac gtagcttgag cggttgacga tgccgatgac
      541 gcgcttcgcg ccgaggtttt tcgccaaaag ggcggacata atgttgcttt cgtcgtcgtt
      601 ggtcagggcg cagaatacgt cgatttcgtc gatgtattcg ttgtcgagca gggtttcgtc
      661 ggttgccgaa ccttgcagga cgagggtgtt gtcgaggttt tcggctatcc attcggcacg
      721 gcgcggccgg cattcgatga ttttgacgtt gtatgcgtgt tcgagctgct tggcgagacg
      781 gtagccgatg ttgccgccgc cggcaatcat gatgcggcgg gtgctggttt ctttggggcg
      841 caattcgggt atgaccgcgc cgatgttttc ggcggcggcg gcaaatagga tttcgtcgcc
      901 ttcgatgatg acggtttgcg gcgcggggac gatgaggcgg ttgttgcggt aaacggcgca
      961 gatttggcag tcggccccgt cgggcaaatc ttgggcgatg tcggcaatgc tgcgtccgac
     1021 aagcagtccg ccgcgccgcg cctgtatgat gaccatccgc acgcggtcgt ctgcaaaacg
     1081 taaaacctgc aatgcgcccg ggcagtctat caggccggca agctgttcgg taacgagctg
     1141 ttcggggctg atggtttcgg ttatgccgaa tatggaaagg ctgccgtttt cgttgttttc
     1201 gagcttgggg ctgaggtatt cgaggtattc gctggaacgg acgcgcgcga tgcggccggg
     1261 gatgttgaac aggtcggcgg caactttgca ggcgacgatg ttggtttcgt cgctgcggga
     1321 gagcgcgagc agcaagtcgg catcttccgc gccggcgcgt tctaatgtga agggggatgc
     1381 gccgttgccg aaaacggttt ggacatcgag gcggctgcct gtttcctgca atgctttttc
     1441 gtcgatgtcg ataacggtta cgtcgttgtt gggtatggcg gcaaggtttt gtgcgacggt
     1501 agaacctacc tgtccgttgc ctaaaatgag gattttcacg gtgtgggtcg ccgagtgatg
     1561 aaatggtgga ttgtaccgca accgggggcg gaaatgccgt ctgaaggcag attttgtgtt
     1621 cagacggcat ggcgcctcaa gattcgacgg ggatgatgcc gattttcgcc tgccatttgc
     1681 gcggggcggt ggcgtggatg gattcgcctt tgctatctac ggcgacggtt acgggcatgt
     1741 ctttgacttc aaattcgtaa atggcttcca tgcccaattc ggggaacgcc aagactttgg
     1801 aagatttgat ggcttttgcc acgagatacg ccgcgccgcc gactgccatg aggtacacgg
     1861 ctttgttgtc ggcgatggct tcgcaggtgg ccacgccgcg ctcggatttg ccgatcatgc
     1921 ccaagaggtc ggtttgttcg agcatttggc gggtgaattt gtccatgcgg gtggctgtgg
     1981 tcggacctgc cggaccgacg acttcatcgc cgaccggatc gacggggccg acgtagtaaa
     2041 tcaggcggtt ggtgaaatcg acgggcaatt cttcgccttt gttgagcata tcgacgaggc
     2101 gtttgtgtgc ggcatcgcgg ccggtgagga ttttgccgtt caacagcaat acgtcgccgg
     2161 ttttccagct tgccacttct tctttggtca gcttgtcgac atcgacgcgt ttgccgttgt
     2221 cggggctgta agtcaaatcg ggccagtctt cgacgcgcgg cggcgtgagt tcgacagggc
     2281 ctgagccgtc caattcaaat tcgacgtggc gggtggcggc gcagttcgga atcatggcaa
     2341 tcggtttgga ggcggcgtgg gtcggataat cgaggatttt cacgtccaac acggtggtca
     2401 gtccgcccaa gccttgtgcg ccgatgccca gcgcgttgac tttttcaaag agttcgaggc
     2461 gcagggcttc ggtggtggac aattccgcgc cggacgcggc cttttcctgc aattcttgaa
     2521 tgtcgatgtg gctcatcagg gactcttttg ccatcagcac ggctttttcg ggcgtgccgc
     2581 cgatgccgat acccaagatg ccgggaggac accagcccgc gcccatggtc gggatggttt
     2641 tcaataccca atcgacgatg ttgtcggaag gattgagcat ggcgagtttg gatttgtttt
     2701 cagagccgcc gccttttgcc gcgcaggtta cttcgacttt accgcccggc acgatgctca
     2761 tatggatgac ggcgggggtg ttgtctttgg tgttttggcg tttgccggcc ggatcggcga
     2821 ggacggaagc gcgcagcgta ttgccttccc aagtgtaggc gcggcgtacg ccttcgttaa
     2881 ccatctcttc cacgctcatg tccgcatccc attggacgtt cataccgact ttgaggaaga
     2941 cggttgcgat acctgtgtct tggcagatgg ggcggttgtt ttccgcacac atacggctgt
     3001 tgaccaaaat ctgcgtcatc gcgtctttgg cggcaggatt ttcttccttc tgccacgcct
     3061 tataaagcgc gtcgatgtag tctttgggat gatagtagct gatgaattgg aaggcatcgc
     3121 aaatgctttg gataaagtct tcctgtttga tgacggtcat gattgtgttc cttttcaagg
     3181 ttgtggcggt aagtgtctgc tgctgtttta aacaaccttt ttgaaggctg ttttttatca
     3241 ggttgaaata gtgccagcgt ttgtacggta aagacaatac ataattctaa tctcttgatt
     3301 atcaatttgt tttaataaaa tactgtttta ctcggatttt gatatttgta atctttgcct
     3361 gttgctttgc ggtagggaga aatcagcagg cagttcattg taggccgatt gatgaacaat
     3421 gccgtctgaa agggcttcag acggcattgt gttcggacgg gcgggcttat tttgcggcct
     3481 gtccgtcttc ttttgcaaaa tatttggcgt aaatcttgtc gtattcgccg ctttcgcgta
     3541 ctttttccaa cgcatcgttc agcattttga cggttgcttc gtcgcctttg cgtaccgcga
     3601 tgccgtagtg ttcggtggtg aagtcgggca gggtaacgaa gtccatccct ttggccggat
     3661 tgtttttcac ataattggcg atgaccgcgc tgtcgctgac cacggaatcc aagccgccgt
     3721 tttccagttc tttgataatc aggggaacgt tttcaaagcg cgcgattttc ggattgtcgt
     3781 tgcccaagag tttggatacg gagaaatcgc ccgtgtagcc ggttaccacg ccgactttgt
     3841 tcatgttttt caaatcttcg gaagaagata cttttttgcc tttcggaacg aggacgactt
     3901 gggtgatttc aaaatacggg tcgctgaagt ccatagactg tttgcggtcg tcggtaatgg
     3961 ttacgcccga catcacaacg tccgcatcgc cgttgtttaa ggcggggaaa aggctgtccc
     4021 acggctggtg tttgaattcg attttaaaat tgcccgcctt cgccatcgcg ttcatcaaat
     4081 ccacatcgaa accttcgaca ttgcctttcg agtctaaaga ttcaaagggg gcaaactcgg
     4141 cgttggaagc cacgcggtac actttgtcgg ggttggcggc aggcgcggcg gtatctttgc
     4201 cctgaccgcc gcaggcagac agcgcgagcg cggaacaggc aagggcggcg gcaatccatt
     4261 ttttcatatt catcaaaatt cctttcggta atcaaaaagc ctaaaaaatc agtttaacat
     4321 aaaattaacg ttctttttca cggaaaaacg cataagtaac gtcgtggaac aaatccgtat
     4381 gcgggctgtc ctcatagctc aattctgcca aggcgtcctc aaaagcaagc tgaatcgact
     4441 cgaccgcgat ttcggcggtt tcggcgggaa gggcgcggat taaggctttg aatgcggtgg
     4501 acaggacgtg gttctgcata cgcagcacgt cgttcgcctc ttccagatat tccaaacgct
     4561 gttcgatgct gcccatgatg tatcctcgcg aatctgttaa aatgcgccat tatatcaaac
     4621 cgcgctttgg gcgcgggttt gtctgcttgc aaagattaag aaccggaagc cgaaaatgga
     4681 tgcgaataaa gagccgaatt gggaaaaacg tttggaaaac ctgcgtcaga cgcgccgtat
     4741 gaaggcagtg ccgacttacg cgccgctgtc ccgcccctct gaaaccggaa cggacgcgcc
     4801 tgtgccgaaa ctggacgatg agagccgagc ccgtgtgttg aaggcgtatc tgaaaaaatg
     4861 gcaggaggaa cacaatgccg ccgttgccga ttgcggcgag gaagtcgaaa ccgacccgat
     4921 gatcgtcctt caggaaaact ggctcaacgc gcaggcttcc ctgcaaatgt cggtttcgga
     4981 aaaacacatc gaatcgcggc ggagaatccg gtttgatacc aaaaaagaca gtgtcgaatt
     5041 tgaaaccgga cagattgaaa gcggggaaaa cgccgacgag gcagaatccg gcacgaatga
     5101 gccggcggtt tcggaaaacg gggaaaacga tgcggaagaa acgttgatgc ccgtccaact
     5161 caatatcctg aacccgaaag ccgtcaaccg gcgcgaagtg ttctgcctgt ccgaacagga
     5221 gctgaccgaa cggctgatca aacgcctgcg cccgcatctg accgataccg tcaacggtat
     5281 catccgtacg gcggtgcaaa agcagatggc ggtttttacc tatcagctcc agcagatgct
     5341 gcacgagcag gcgggtgcgg tagtcgaaga cgtgttggaa cacgatgtcc gcaaaatcct
     5401 caacgacatc aagtacgagt taaaatacaa acgctgattg ccatgccgtc tgaaagccgt
     5461 ttcagacggc atttgcattc tcacccgact tgaaagcatc atagaacagg cgcaaaatat
     5521 taacaaatta aacaacgggc gcacgccccg tttttgaacg gacaaaaccg aggaatcacc
     5581 gatgagcaaa aaactccatc cgcaaacgct cgccatacgc ggcggcaaag aacaaaccgg
     5641 ataccgcgaa cacaatcagg cattgttcct gaccagcagt ttcatgtggg acaacgccca
     5701 acacgctgcc gatttgtttt caaaaaaaat caaaggcttc acttataccc gtaccgccaa
     5761 cccgaccacc gccgcctttg aaaaacgcat cgccgctttg gaaggtgcgg aacgcgcggt
     5821 cgccacttcg accggcatgt ctgcgattca ggcggcgttt ttcacctttt tgcaggcggg
     5881 cgaccacgtg gtttccagcc gcagcctgtt cggcacgacc gtcggcttta tcaataacat
     5941 cgttaccaaa ttcggcatcg aagtgagcca cgtgtcgccg actgatataa acgaatggaa
     6001 agccgccgtc aaagccaaca ccaaactgct gtttttggaa acgccgtcca atcccttggg
     6061 cgaagtggcc gacttggaag ccttggcgga attggcgcac ggcatcggcg cgattttggt
     6121 ggtggacaac agcctgctgt cgcccgtcgg ctcgcagcct ttgaaacacg gcgcggatat
     6181 ttccgtttcc tccgcgacca aagccataga cggacacggg cgcgtgatgg gcggcgtgtt
     6241 ggcgggttcg gaagagctga tggcgcaggt cgctatgtat tgcaactctt gcgggctggc
     6301 aatgtcgccg tttaatgcgt ggcagttgtt gagcggcgtg gaaaccctgt cgctgcgtat
     6361 ggaaaaacag ttcgacaacg ccctgaaaat cgcgcaatgg ctgcaagcgc agccgcaggt
     6421 tcaagccgtg tactacaccg gattgcccga ccatccgcag gcggcgctta tccgcaaaca
     6481 gcaaaacggc ggcggcatcg tcatcggctt tgaagttgcc gaccaagcag ccgcgtggaa
     6541 agtcgtggac ggcgtggaac tcttttcccg taccgccaac ctcggcgacg tgcgctcgac
     6601 catcacccat ccgtggacaa ccacgcacgg caggatgcag cccgaagaaa aactcgccgc
     6661 caacatccgc cccggattgg tgcgcctgtc ggtcggtttg gaatatgttg gcgatttgat
     6721 tgacgatttg aaacaggcac tcgcgcgctg attgcgtgtt tcagacggca ttggacagca
     6781 gatgccgtct gaaaccctta tcgcgtatgg catacacatc aacaatagga aaaaatatgg
     6841 atgaagcagt ttttgccgat tgggcgttga aaatctgcct gaccggcttg attatttttt
     6901 tgggttttat cgtttggaat ttgggcaagg agtccaaagc aggcaaattc ggcatcgcca
     6961 ttttgttttt ggttttgggc ttgggcgtgt tcggttttgt gttcaaagag ttattgatta
     7021 agtttttggt attgccgaaa taggtttgcg gtctgtcaaa atgctgtctg aaatcataaa
     7081 aagtaatctg caccctaaaa gtttgattta accgccaact gattaaggtg cggattttta
     7141 tgacaaaata aaaatcatct ttccgcagtc attcccacgg acgcgaaagt ctgtcttttt
     7201 gaatcccgga taattttaca tcgtcattcc cgcgcaggcg ggaatccaga tcattgggta
     7261 gcggcaatgt tcaaaagtcg tctgaaaaat cagaagttct agattcccgt ttttacggga
     7321 atgacggaat ttcagacggc atcctcccac cccgtcattc ccgcgcaggc gggaatctag
     7381 tccgttcggt ttcggtcatt tacgataaat tcctgttgct tttcatttct ggattcccac
     7441 ttccgtggga atgacggagg agcggataaa ttctcgcaat ctaaaacctt gtcatttcca
     7501 taaaaaacag caacccgaaa cagcaactta aaaacagcaa cctgaaacct cgtcattccc
     7561 gcgcaggtgg gaatccagat cattgggtgg cggcaatgtt caaaagtcgt ctgaaaaatc
     7621 agaagttcta gattcccgtt ttcacgggaa tgacggaatt tcagacggca tctgcccgcc
     7681 ccgtcattcc cgcgtaggcg ggaatctagt ccgttcggtt tcagtcattt acgataaatt
     7741 cctgttgcct ttcatttcta gattcccact tccgtgggaa tgacggtttg gaagttgcct
     7801 gaaactcgaa aacaactata gtggtttaaa tttaaaccac tataaaactg aatgaatcgg
     7861 attcccactt ctgtgggaat gacaaacttt aaggtgtgat gacttatcca aaacagtcga
     7921 aacgcaaaaa ccggtttctc gtttgcataa gaacggcaaa tttttcggtg tcttgtttta
     7981 tgggcgttat cccttgtccg tcccgtaggg caggtaaacg tcgaagcggg tgcttttgcc
     8041 ggtgtattgg taggcagggg cttgtccggc aaggtgttcg ccgaggcggg ggcgtttgac
     8101 gacgacgcgt ttttttgctg tttggcgtgc ggtatgcagg agtgccgctt catcttgcgc
     8161 ttcgccgacg agccgatgga agtaggtcat ttctttttta acggcggcac ttttgcggcg
     8221 ttcgggatac atggggtcga gatagacgat gtcgggtttg ccttgtgttt gaacaagggc
     8281 gggcatttgt tcggcggcgt tgccgaaatg gaggttgatg tgcgcggcgg tgtcttgcgt
     8341 ttcgggattg aggagggcgc ggcggatgcc gtctgaaagc aggcaggcga cggcgggatg
     8401 ttgctcgaag gcggtaacgg ccagcccgag cgaggcgagg acgaagctgt cgcgccccaa
     8461 tcctgcggtt gcgtcccaaa cggtggggtg cgcggtgtgg ttgacggctt tggcgatgag
     8521 ttcgcccccg ccttttgtgc gccggtattg tgccgcgccg gaggcaaaat cgacgatgac
     8581 gctgcttttt tccccgacaa gcctgaggct gacggtatcg tgttcggcaa ggaggtaact
     8641 gccttgttcg ggcggttggg aaacgggcac aagggggaat gcccgtatca gggcgcggac
     8701 ggcttcggtg gcggtgttgt caataaggat gtcggtcatg gtgttttgtg tccgttcggg
     8761 cgtggggcga acccgctttc ccgtgccgtt tcagacggca ttttttcggg tgggcggaga
     8821 tggtgcggat gccgttttgt cgtcagccga tgttgaggcg ttccatacgg tagcgcatgg
     8881 aacggaagct gatgcccaag cgtttggcgg cctgcgtgcg gttgccttcg gtttgtttga
     8941 gggtttgttc gatgatgtcg cgttcgattt ggtcgagata gtcctgtatc tgcatggtat
     9001 cggggtcgaa cgcgccgcaa cgcagggctt cctgtgcctg atcggcattg ccgaaggcgg
     9061 tgatgacggc aaccggcgta tcgagcaggc ggctgttgat gtgttggacg acttcgagcc
     9121 ccgagccgtc cggcatacgc atatcggtca ggacgagcga atagcgttgg ctgtcgagct
     9181 tgtttttggc ttcggcaacg cctgacgcgg tatggacgcg caagcccatt ttcatcaggg
     9241 tcatttccat caggtcgaga atgtcggttt cgtcatcgac gacgaggacg gggtgttgca
     9301 gttcagtcat tggttttttc cggtaatgtg agttcgaaac atttggcttc cggtaggtag
     9361 gtcaaatcgc cgaaattggc gtgcgccagt tcgcgggcga catacagccc gaggccggtg
     9421 ccgttttccg ccgtggtgta aaagggttcg aacaggtgtt cctgcacttc gggcggtcgg
     9481 caaagaggat acagacggtg tttttttgcg cggggcggat ggtgacggaa atcgagccgg
     9541 gctgtttgcg gctgtgccgc cacgcgttgt tggcgaggtt ccacataatt tgccgcaggt
     9601 gggcaggatc gaaataggca gtcaggtgat tgccctgcat atctaggcgg atgcagccgg
     9661 cggcatcggg atggccgagc aggaactctt gtttgaattc ttcccaaaac ggtatcaggc
     9721 cgatggtttc gcgttcggtt ttgttgcgct tgttgagcga ggaaatatct tcgagcattt
     9781 tgtcgatacg gcagacgttg ccgtcgatga ttttgcacaa tttggcgttg aacggatctg
     9841 ccgcccccgc ttccatattt tcgcgcaaca ggtcgtttgc atggcggatg gcggacatgg
     9901 ggttgcggat ttcgtgggcg atgttggcgg tcagttgtcc gagcgcggca agtttgacgg
     9961 acagggcttc tgcctgaatt tcgctttgcg ggcggatgta gaggatgagc agcttgttct
    10021 gctctttgtt catcggcacg gcgcggatgc gggcggtcag ggcgggcgtg tcgatattgc
    10081 gttcgaaagt acgcgaaggg gccttgtccc ataaaacggt aatagggtcg aacagggcgg
    10141 tatgctgtcc gatttcaagc gtggggagca gatcttttgc ctttttattg aacagtacgg
    10201 tctgatactc gacgttgatg atgacgaccg cttcctgaac gcggttgagc acgatttggt
    10261 tcaagcccct gatgcggcgg taggcggcgt ggttttcatg ggcaagtttg ccggcacggt
    10321 cgatgtatct gaccgacagc gaggcaatca tggcgacgaa ataggagccg gtcacgagga
    10381 cgaaggtgtt ggtgacggtt tttgcatcca aaatcagcgg atacatattg atattgctgt
    10441 ctgcaagggc attcaatatc agcaggatgg cggcgtagct ggcatagagc agggggtaac
    10501 gcccgtaact gagcaggcag gagctgccca cgaagggcag gatcaggatg ccgaaccccg
    10561 agttgatgcc gccgaacagg taggtcagta tgccaatcat ggtaatgtcg gacacggcgc
    10621 tgaaactcgg tatcttcaaa gcctgccatt gccaatgcgg atggatgatg gaaaagaaaa
    10681 tcagccagca ggcaatggcg agccaaacat aaaacgccca cgccgtccac gaagcgtgca
    10741 ggttcgcacg gctgccggtt tccagcccga ggatgtgcat aatcatcagc ggaaaaacga
    10801 tggcgacgcg gatgatgttg atcagattgg gaatccggtc tttgagtttt tcaagttcgc
    10861 gggggttgga aatcaccata attatgtgtg gaatcgggtt tatttggggg aagaaagctg
    10921 tttcaggctg cccgatatgg gtaatagttt gccttttttg ccgcgttttg cctcaagcag
    10981 ggagacgggg atgcggtctt tgtgcgccgc gccgcgcctg ccttcgcttt caatcaggat
    11041 ttccggctcg gaagaaacgg cggtatgcgt cagcgattcg ccggcgttta atccgatgat
    11101 ttgcagccct ttgcctttcg ccataatttt caattcgccg atggggaagg cgagggcgcg
    11161 gttttgactg gtggcggcaa tgattttgca gtcggggttg atgaacgagg aggcatagac
    11221 ggcaatcggc ggcaggacgg tttcgccact gtctgtggtc atcaccactt tgcccgcttt
    11281 tacgcgtccg accatatcag ccaacttggc gatgaagccg tagccgccgc tgcttgataa
    11341 taaataatgt tgttccggca atcctgtcaa catcgcaacg ggtttcgcgc cgttttgcaa
    11401 ctcgatcaag gatgaaaccg gcacgccgtc gccgcgtccg ccgggaattt cggcggcatc
    11461 gagtgtatag gttctgccca atgaatcgag gatgacgacg ggtaaaaccg ttctgccttc
    11521 aagggtttgt ttgaggcggt cgccttcttt gaacgcggtt tggctcaaat cgagattgtg
    11581 tccggcacgg ctgcgtatcc agcctttttc cgacagaatc agtgtgatgg gttcgtcggc
    11641 ggtggtttgt gtcagcacgg cgcgtccggc ctcttccacc agcgtgcggc gtgcatcgcc
    11701 gtattgtttc atatccgcct gcatctcttt gataatcagc ttgcgctttt cgttttcatc
    11761 gcccaaaagg atgttcagac ggccttgttc ttcgcgcaat tcgttcaatt ctttttcgag
    11821 tttgaaacct tccaaacgtg ccaactggcg caggcggatt tccaaaatgt cttcggcttg
    11881 gatttcggtc agcccgaacg ccgccatcaa atcggctttc gggtcatctg attcgcggat
    11941 gactttaatc acttcgtcga tgtgcagaaa gactttcaga cggccttcga ggatgtgcag
    12001 ccgtttttcc acttggttca aacggaattt cagacggcgt gttacggtta cggtgcggaa
    12061 atccagccat tcctgcaaaa tcgttttcag gtttttctgc gcggggcggt tgtccagccc
    12121 catcatcacc aagttcatgg acacattgcc ttccagcgaa gtttgcgcca tcagcgtgtt
    12181 gatgaaggta tcggtatcga tgcggctgga tttcggttcg aacaccagtc gcacgggatg
    12241 ttcgccgtcg gactcgtcgc gcacgcggtc gattaaatcc agcatcagct ttttggtatt
    12301 gagctggtct tggttgagct gtttcttacc cgctttcggt ttcgggttgg tttgctcttc
    12361 gatttcggca aggattttgg cggaattggc gttcggcggc agctcggtta cgatgacgcg
    12421 ccactgtccg cgcgccaatt tttcgatttc ataacgcgca cgcacgcgca cgctgccctt
    12481 gccggtttca taaatccggc gcaattcgtc cgccggcgtg atgatttgac cgccgccggc
    12541 aaaatcgggg gcaggaatat attgcatcag gtcggcggtt tccagcgtcg gctttttcaa
    12601 caacgcaatc gccgcctgcg tcacttcgtt caaattgtgc ggcggaatct cggtcgccat
    12661 gcccaccgca atgcctgacg cgccgttgag caacaccata ggcaggcggg cgggcaggtg
    12721 cagcggttcg tcaaacgcgc cgtcgtagtt cggcacaaaa tccaccgtcc cctgattgat
    12781 ttcggacaac agcaattccg caatcggcgt cagccgcgct tcggtgtaac gcatcgccgc
    12841 cgccccgtcg ccgtcgcgcg agccgaagtt gccgatgccg tcgattaagg gatagcgcaa
    12901 ggtaaaatcc tgcgccatcc gcaccatcgc ctcataggcg gaactgtcgc cgtgcgggtg
    12961 gtatttaccc aaaatctcgc cgaccacgcg cgccgatttc accggcttcg cccccgccgt
    13021 caaacccata tcgcgcatgg caaacaaaat gcgccgctgc acgggcttct ggccgtctga
    13081 aacttcaggc agcgcgcggc ctttgaccac gctcatggcg tattcgagat aggcgcgttc
    13141 ggcgtatcgg ccgagcatca gcgtattgga atcggtatgt ggggcgtgcg cttgcgtatt
    13201 cattgtgtat gcggaatgta aagcaaagga cattattgta aatcaaaacg gcaaaccata
    13261 gtgggcgcgt gtctgaatct gaatcaaata cgggaaatgt gaaaatatgt tataaataag
    13321 gctttcccac tttcacacat tggagacgat atggaactgg tatttatccg ccacggacaa
    13381 agcgaatgga acgcgaaaaa cctgtttaca ggctggcgcg acgtgaagct gagcgagcag
    13441 gggcttgccg aggctgccgc cgccggcaag aaactgaaag aaaacggcta tgagttcgac
    13501 atcgccttca catccgtcct gacccgcgcg attaagacct gcaacatcgt tttggaagaa
    13561 tccgaccaac tgttcgtacc gcaaatcaaa acgtggcggc tgaacgaacg ccactacggc
    13621 caactgcaag gcctggacaa aaaacaaacc gccgaaaaat acggcgacga gcaagtccgc
    13681 atctggcggc gcagctacga caccctgccg ccgcttttgg acaaagacga tgagttttcc
    13741 gcacacaaag accgccgcta tgcccatctg cctgccgatg tcgtacccga cggcgaaaac
    13801 ctgaaagtaa cgctggagcg cgtattgccg ttttgggaag accaaatcgc ccccgcgatt
    13861 ttgagcggca aacgcgtctt ggtggcggcg cacggcaact ccctgcgcgc gctggcaaaa
    13921 cacatcgagg gcatttccga cgaagacatt atgggcttgg aaatcccgac cggtcagccg
    13981 ttggtgtaca aattggacga caatttgaaa gtcatcgaaa aattctacct gtaagggtgt
    14041 gaaataaaaa atgccgtctg aatcccagcg tttcagacgg catttttgac aaggcggcaa
    14101 aacgcatcag ctgcgtacca agaaacgccc cgtcgctatc cggtgcagcg taccgacaag
    14161 cagcagcgcc acgcctgccg atccggcggt ggcaagcgtc agccccaagc ccgacaatac
    14221 gccggaagcc gccagatgca gaccgcatcc tgccattgcc gcgaaaccga atgaaaatcc
    14281 ccaaaagctc atcgtaaaac caccttccca aaaccagcgg gtcaggcgca gcaagaacaa
    14341 aagctgcagg cagccgtagc cgattaatgc caacgcaaaa ccgtcgactt taccgccgac
    14401 ggcaaaatac gcgccgcagc cgacaaaggc gggcgcaagc tggatgccga ccacgccgcg
    14461 cgccgccgtg ccgaccggtg ccgccgtgcg cagcctgccc aagatggagg cttccaagct
    14521 cagccaggaa aacatacccg cgccgaaaaa caaagctgca taatcatgat gccccaacgc
    14581 cgccagagat gacgcgctga caaagtttgt cgcaaccgta ggcagataaa taatcggcgt
    14641 cgtcgcctcc aaggaatgca ggccgcgcca cagaccggcc gcccgataca tcgaaaaagc
    14701 caactgtccg gcaacgccga cataaatcag gactgccgcc gcgcctgcct gatagggctt
    14761 cagcgcgagt ccctccagca tagcggtaat cggaatcgcg ctgatgaagc agcattgcac
    14821 caagtcgcgt aaatcagaca aaaaatcgtt tcggtacgca aacattttga tcaggtatgc
    14881 cgccaccagc aagagccaga cgaccgaagc cgccgcaagc agcgattcgg cggccaaggc
    14941 gggcagcagc ccgacagacg cgccgtaacg ccacgacagc cccaaggcaa acaagcccag
    15001 cgcgatgctg aaatagctca aaggaatcgg aaatttctta gatgcagaca taaacaccct
    15061 tcagacggca tgccgtcgta acgcaca
//
LOCUS       DAWHRS010000035        14911 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWHRS010000035 DAWHRS010000000
VERSION     DAWHRS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.035"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(657..2630)
                     /locus_tag="WIB82_000757"
     CDS             complement(657..2630)
                     /locus_tag="WIB82_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ3842088.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVITGDQTVNGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIQGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     ALGYEDRNERQRSALVSTWTDENLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSERVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(2641..3201)
                     /locus_tag="WIB82_000758"
     CDS             complement(2641..3201)
                     /locus_tag="WIB82_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ3842089.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQHRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(3198..3809)
                     /locus_tag="WIB82_000759"
     CDS             complement(3198..3809)
                     /locus_tag="WIB82_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ3842090.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVSSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            complement(3809..4252)
                     /locus_tag="WIB82_000760"
     CDS             complement(3809..4252)
                     /locus_tag="WIB82_000760"
                     /inference="COORDINATES: protein motif:HMM:NF016736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ3842091.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLSRRNPTTASGTLTVSGIAQSMLSDD
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARRN"
     gene            complement(4266..4613)
                     /locus_tag="WIB82_000761"
     CDS             complement(4266..4613)
                     /locus_tag="WIB82_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ3842092.1"
                     /translation="MDKELNPGTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            complement(4714..5382)
                     /locus_tag="WIB82_000762"
     CDS             complement(4714..5382)
                     /locus_tag="WIB82_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ3842093.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(5379..6523)
                     /locus_tag="WIB82_000763"
                     /pseudo
     CDS             complement(5379..6523)
                     /locus_tag="WIB82_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(6513..7844)
                     /locus_tag="WIB82_000764"
     CDS             complement(6513..7844)
                     /locus_tag="WIB82_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ3842094.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGTTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(7844..10015)
                     /locus_tag="WIB82_000765"
     CDS             complement(7844..10015)
                     /locus_tag="WIB82_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ3842095.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPDTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALGWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            10170..10772
                     /locus_tag="WIB82_000766"
     CDS             10170..10772
                     /locus_tag="WIB82_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842096.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLFTALTEVV
                     KESNQTGATAQKDVPADGILYSVVFEKETNTIAIIGRKQP"
     gene            complement(10816..11049)
                     /locus_tag="WIB82_000767"
     CDS             complement(10816..11049)
                     /locus_tag="WIB82_000767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842097.1"
                     /translation="MRRRRRSSKTQLPDGTQKLPSGGHPDGKAGIGADAVRDMCHAEL
                     SAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(11024..11389)
                     /locus_tag="WIB82_000768"
     CDS             complement(11024..11389)
                     /locus_tag="WIB82_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842098.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     RLKPSAATASPDAPSAAEQ"
     gene            complement(11393..11770)
                     /locus_tag="WIB82_000769"
     CDS             complement(11393..11770)
                     /locus_tag="WIB82_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ3842099.1"
                     /translation="MSDATYADAVIMEMNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEDRRTSYLDCFTVDTG
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(11835..13244)
                     /locus_tag="WIB82_000770"
     CDS             complement(11835..13244)
                     /locus_tag="WIB82_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ3842100.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVADRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANAFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVSNAIEQRGCIGVLGMSAALSTATTATGEINDGRM
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPLFAECNNA
                     LYNGLTPLTVVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(13247..13444)
                     /locus_tag="WIB82_000771"
     CDS             complement(13247..13444)
                     /locus_tag="WIB82_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ3842101.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(13441..14091)
                     /locus_tag="WIB82_000772"
     CDS             complement(13441..14091)
                     /locus_tag="WIB82_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ3842102.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(14081..14503)
                     /locus_tag="WIB82_000773"
     CDS             complement(14081..14503)
                     /locus_tag="WIB82_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ3842103.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(14503..14901)
                     /locus_tag="WIB82_000774"
     CDS             complement(14503..14901)
                     /locus_tag="WIB82_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842104.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
BASE COUNT         2873 a         4326 c         4059 g         3653 t
ORIGIN      
        1 gttttttccg atagattcct gccgcgtttg gggtctggat tcccgcctgc gcgggaatga
       61 cggcggcggg gatttctgtt ttttccgata gattcctgtt gcgtcggggt ctggattccc
      121 gctttcgcgg gaatgacggc ggcgggggtt tctgtttttt ccgatagatt cctgccgcgt
      181 ttggggtcta gattcccgct ttcgcgggaa tgacggtggc gggggtttcg gtttttccga
      241 tagattcctg ttgcgtcggg gggtctggat tcccgctttc gcgggaatga cgaaagcggc
      301 gggaatgacg aagagtggca ggattggcgg ggcataggtt tcgggggatt ggcggcgcgg
      361 ccggtttcgg tcggattgct tggatcggcg ggctgaagcc catctctaca actcgtctct
      421 acaacccatc tctacaaccc atctctacag ccatctctac agccatctct acagcccatc
      481 tctacagccc atctctacag cccatctcta cagcccatct ctacagccca tctctacaac
      541 ccatctctac agcccatctc tacagccatc tctacagcca tctctacaac ccatctctac
      601 aacccgtctc tacagccatc tctacagccc gtctctacag ccatctctac agcccgctag
      661 tcttgcagtg cctcgagcaa gccgtctcgg cggcggcggt ttcggggtcc ggttgatgtt
      721 cttccactct ctctttgcgg ttcttcgttg gtttcaggta tttcggggcc tatcggggag
      781 ctggcggcgt ttcccgaggc ggctttgccg atggccatcc agtcgcaact gccttcgtac
      841 cagttggtga taatggcggc ggcgtggttt tttgcgcgga taaacagcca gttgtgttgc
      901 cttcgatcgc cggtaacgcg ttccgaatgg cgttcggata cgaagcattt gacgttgccg
      961 tcggcaaagg cgacggggaa gacgacttcg ttttcgatgg ggccgccgct tcgtgtgaac
     1021 ctgtagctgc cggtctgtat cattgtgccg tcggggaatc tgacaatatc cacgccgccg
     1081 atggtttggc gtgtgaattg cgcggcaacg gcggtgctga ttttttcgtc cagcccttgg
     1141 atttgggcgg cggtgtgggt gtggtctcgg tcggctttgt cttgcaaacc ttgcgccagc
     1201 gtgcccgcgt ccagcgcgcc tgcgttgatg gtttcgccgt gggactttat ccaaaacacg
     1261 gcttcgccca aggtgtcggc ggctttgatg cacagtttta aaaccagggc tttggggcgg
     1321 ttttcgtcgc cgcctgtcgc cattttgctg tccgagcgcg gggttaaaaa gccgttgtcg
     1381 tttaaatttt cgtccgtcca agtcgatacg agcgcgcttc tctgcctttc gttgcggtct
     1441 tcgtaaccga gggctgccgc gtctgtgtgg tttgtccagt gtgaaaatac tttgtggacg
     1501 tgccgtttga tttcgtcttc ctgcttcgtt ccgactgcca agctgttgcc cgcgttgcgg
     1561 ataaagcggt cttccgcctg cgggacgttt tggatgctgc cgtattttcc ggtcagcaga
     1621 cggtacagct cgggataagc ggtttcggtt acgcgcgtgc ggatgtcgtc aaacgccagc
     1681 cagccggtcg ggatttggtc ggacggaaac cacgcggtga tgccgatgtc ggtacggctt
     1741 aaatcgggca ggcggttgct gttgcccaag gcgcggtaaa ggtcggggaa ggtgttttgt
     1801 gcaaaggtcg tgccgttggc tttgagatag cctgcggggt tttgtacggc tttgggaaag
     1861 gatacgatcg cgccgacggg gatgccgtcc gaattgagtt ttttccatgc ggaccagttg
     1921 tcgtttgcgc tgctcgtttg gtggcgttcg tacacgtcgg acgtgtaggc gggataaccc
     1981 aactgcctgc accagccgtg tttcgtgccg gcgatgactt ggatatggca ggcggtgttt
     2041 tcaacgggca ggttggagct gccgaccgcc gtcggcaggg aatagatgcc gtctgttttg
     2101 agggtgttca aatcgccccg gaatgtttcg actttgaaat tgccgatgcc gtatccggac
     2161 agtgtgttcg gtttgccttg gatgtcttcg gcaaaacgcg gtttgttggc tttgtggaag
     2221 atgtcgtggt tgttgtagga aatgcggttg tttgcgccgt gcaggcggag ggtgtcgtcg
     2281 ccgaagacga tgtgggcgtt gtcggcatcc gcgccgatgt aggctttgtg gccgccgttc
     2341 cagttcgtct ggttggcgga taaatggatg ccgctttgga attgggtttg ggcggtaaag
     2401 gttttttggc cgttgacggt ttggtcgccg gtgataacta cagccagcct atttagtgct
     2461 gccagtaatt gattaggctg cgacttatcc ggctggatac cggccgcagt cagcacggcc
     2521 agcagctcgg attgcacttg gttgagccac cacgcgggca ggatggtgcc caattcggac
     2581 acgccgtcgc cgtcgtggaa ggtcttatcg ggggtttcga tggggtgcat aaatcgtcct
     2641 ttagcggtag gtaaatcgga tagcggtgtg ggcgggcttg aggcggttga acaggctctc
     2701 gatcacggca tcgctgtaat cggtcagcct gtcgcccgcc gccgagatac cggcgcggaa
     2761 tcgggtaatg cggttgttgc cgccgcgcac gttaacgtgc cacacccaca tgatttcctg
     2821 cggcgcaaga cggtcgccgg cgcggtttac accggcgcgg aacggctgcg gttcgtcgat
     2881 ttggatttga tagcccgccg cctcggccaa acgcacaaaa taaggaatac tcaagccgcc
     2941 tgtttcgttt agcttggcca tgacggccaa cacacggtgc tggcggtttt tgcccgtacc
     3001 gtccaaacct aatacgcgct cccaatcggc cagcatttgg ccggcgctgc gcgggtcgac
     3061 ggcatcggca acgctttgag ccgattccgc caccgcatcc agcgctgcgc cgtctatttc
     3121 tgcctgcgcc cgcacacgcg gggcattgcg ggcatacgac acggggggca acaggccccg
     3181 caagatgtct tgatagctca tgacggcatc tccgttacat taatcgcgcc cgctttaaac
     3241 cactcgatgc ggttaaccgt atcggcggca cggttggccg tcggcgcagt cagacggcgg
     3301 tcgatcacac catccacatt gctgatagca gcctcgattt gcgacacagt caggccgtcg
     3361 ccgggaatca gggtgtcgaa atattctttt agggccgtcc gtatgcggcg cttggcctcg
     3421 tccaagtcga taccgtcgag cttgacttgc acggtaacag gcaccgccgt tacggttggc
     3481 ttgagtacca gcgcattttt tgccgttacc gggcgcatct cgtcgatata agcctgtacg
     3541 cggcgcacag tttcttccga cgacacaccg tcggcggagg taatggcaat atccaccgta
     3601 cccaagccgc ggcgcagcgg ataaacatat gcgctggtta cgccgtcaac actcaacgcc
     3661 cagtttttat agtcgtaacg gttgccgcct gcgggcggtc ggcggatgat ttccaacaga
     3721 cgcgccagca gtgaggcatc gctttctcgg tcggtgccgc cttgtacggt aaggcggcat
     3781 tcggtggcca caccggcggg ggcggccatc agttgcgcct cgccgtcgcc acattggcgg
     3841 ccgcgcccgg ctcgtcggcg attgccggta tttccgccgt gccgccgctg ccgataacgg
     3901 cgcgggcggt agtgcggtaa aaacgctggc cgatacgcac ttgcaggtcg tctgaaagca
     3961 tggattgcgc aataccgctt acggtcagcg tgccgctggc cgtggtagga ttgcggcggc
     4021 tcaagccgcg catggaggca tgccgctcca aatactcgcg gtcggcggta tcagggaaaa
     4081 tctgccgcac aatccagctt tgatgcgcat attgcccttc ggcgcagctg gccaaacggc
     4141 tggcgtgcac ataatggtcg ctgtcggggc tgatatcggc atccggccac aggcttttgg
     4201 tatcgcgcag gatacgctcg cggatttgct caaatgtcgg cgtttcaaac acgtttaaaa
     4261 cctctttaaa tcacgggcac ttcgtggcgg taatcaaacc cgcccgccgc cgtatccacc
     4321 cggatatgca ggatcaggcg gccgtcgtgc ggctgctctg cgcgcacggt aatcttgtcg
     4381 gcacgcccgc tcttaacaat cggttgcagt gcctcatcgg catattgctc ggccaacagg
     4441 ctgacccgtt gcaggtcttt ttcgcgctgc aacaaatgca gcagcgagcc gagcgtttta
     4501 tccgcccacc agctgcccaa cggtgtcatc aaacggatat acacggcatt ttgcagcgta
     4561 tcgacggtgc ggccggtata gtcgccggtg ccggggttta gctctttgtc catgttggca
     4621 ttttgccgtt ttcagacggc ctcacggagt acacgggctt caatcccgca cgcacaaaaa
     4681 aagcagcccg aaggctgcca aaggtctgct tgtctatgcc ggttccgctg gtaaggtttt
     4741 gccgccgatg ctgtcggtat gcgggtgctg gcgcaacgat atattgccgg ccaccacgtc
     4801 gccgtcggtg ttaaagctgc cgcccgtttg gttaacatcg ccgctaaagg tggctccgtc
     4861 gccgccctcg acggccatgc cgccgttgcc gttgatttgg ccttgcgccg tcaacactgc
     4921 actggtctcc accaacggag cgttaaattt ggcatccgtg gccgcattaa cctcgtattg
     4981 tttgcagtta acccggtaca cgtcgcaatc ggcctcaatg attttgcctt gcttaatcac
     5041 gatttttgca ccctcatgat taaaaatcgc cgtctcgccg ggcttaaggt ttttgatgcg
     5101 gtagctgccg tgctggctgc acacaatcac accgtgcgaa gtattgccgc ccagcggtat
     5161 cactaccgct tcgctgccgt cgggcggatg gctggcaaag ccgtattcct gcaaatgttc
     5221 aaggtcttgc agggtttcgt cggccaagcc gctcaactgc acgcgctgta tcggctcgga
     5281 cgacaccacc agcgtgattt ttccccgaaa ggccgcgcgc agggtttcgc cgatattttt
     5341 agcagtttgt gccgtttttt tcgccaattt actcaaactc attcaaacac cgccgtttct
     5401 gcttgttttt tgccgccttt tttgccctta tggctcacgc ctttgcgttt gcctttgcgc
     5461 ttgcgcgccg cctcggcctt tttggggtaa gcgtcgggtg tccaaatacc gtcctctttg
     5521 agccgcagct cggtttgcgt gccatccatg cgggatagca taaaccgccg ccccatcaga
     5581 aaaaacaccg catcgatacc gtgctcgtcg tcgatcacat gcacacgctg gccaggttgc
     5641 cacaatacgc cgtcgcgggt tttatggccg cccacggtta tcgtgagtgt caatccctcc
     5701 agccgccagt cggccagctg ctttttagcc tgcttttgca atgcggccaa attgtcggca
     5761 tcggacacca ccaccgtttt agggcggtgc agcgtcatcg tcgggtcttt gtacacccac
     5821 ttaaatcgtg tttggcgctg tcgccgctgc ggccgtgcga ttgcgccaaa aaagtaacct
     5881 cggaaaagcg gttgtcggta tcccactcaa tgtccatgcg ctcgatattg cggcggctgt
     5941 cggtgcggct ccaacacaat gtcgccaccg gcgggctgct gtaatccgca ccgcccacca
     6001 ccaacgtgcc gtccggctcc agccacggat gcagcccgac cgagttggca atatgggtta
     6061 atgcctgcca tacggtttcg cccggctcga tgtcgatttt gtccaaagcg gggttgtttt
     6121 cggccttaag caccaccgct ttaatctgcg gccacggcgc ggccagcttt ttggctgcat
     6181 ccaataccgt catgcccttt acattgagct gcggcgcgga gcaatccacc aaaaaaccgg
     6241 ccaaatcacg cccgctcaag ctcaactcgc ggccgccctt gcttttgccg tggcgctggc
     6301 tgccgatgat gcccgtcatc acgatttgcc cgtctatcac tacctcgcag ctctctccgc
     6361 ttaaatcggg tatggccgcc tccggcccca acctgccgat gacaaaatcg aagctgtcgg
     6421 cagggattaa aaagtcgctg tcgatgtcgt agcgctccca gtggcggtgc tctttaccgc
     6481 ccacgcgcac cgacacggca tagccgtatg aattattttg catagctgtt gaccaaagtg
     6541 ccgcgcttga taaacgcggg gtggtggata tggggattga gccgcaccag ctctgctgcg
     6601 cgggctatat cgccgtaaaa ctcgtgggcg atttggtgta tcgtaccgtc gattggggct
     6661 tggcgcacaa tcagcggcgg cttttggttg atgaccgccg caaccaacgc attcagacgg
     6721 cctgccgccg cgcgcaggga ttctgccgtt tggtaagcct cggtatacac ggcgttggcc
     6781 gtcagcccac cagactcggc agcagccgtc tgcaccgccc gtaaggcggc aatctcggcc
     6841 tgcatacggc ggcgcatggc gcggttaacc tcaatcaaat cgggcgcggt catctcttcg
     6901 ccatgcgcct cgattaatgc cgttgccacc gacaacagcg atgacgtgga taacaggcgc
     6961 accgcctgcg ctaccggctg cacctgtttg gcggttaaac ggttcaggcg gttttgcagg
     7021 ccgtctgaaa agcggccggt cagcaaatta tcagggatag cggcggcacg gtcggcaaca
     7081 gccgcagccc cgtcaaaccg ctgtcgcggc gaccaaccgg catggtgcat ggcattatcg
     7141 gccaaacccg cctcacggcg tatgccagta tctaccatga ccgatatatc cgcaaacagc
     7201 ttggccgagc cgtttttaaa cgccgctgcg ctgtatccgc cccgatcggg aaaggcaatt
     7261 ttgtccaaat caaacaaacg gcgcaccgcg ccaaacgtac ccgatgccgc actccaaatg
     7321 cccagcgcgc tgccccataa agccgatacg cccgcatcca ccgccaacac cgcatcaaca
     7381 aagccgatag ccgcctcgcg gtaggtgtcg atatcagcaa tcaacgcctc aagctcgacc
     7441 aaaaaggcgt tttcaaaaac aaagatttcc tgcgcttcgg ccgcctcgcg gaaagtaata
     7501 tcgatgcccg cataatccac ataatcggcc tcatgtcggt agctccatga tgccgcaatc
     7561 atgttgtgca tccgccccca aacagggtgc accagcacgc cgccgcccgg ctgctccagc
     7621 gcatccagca gctttttcag acggcctgca tagcccttgc cccaaaacac cgcattaatc
     7681 tgcacctgcc gcccggtcgt gcccatgtct tcaaggtcga taccctgcac aaacggccgc
     7741 gcatgctcgg ccaatgcctt gccgttgctc tcgtccacca cctcaatatc aaagccgacg
     7801 cccttgtaag atgcgtcctg caataaggta tgccatccgc tcattattga cccgctccac
     7861 ggccgaacat ggccacttga taccgcgata cctcattcgc gataacacgt ccgtcgatat
     7921 tcaggctcac attggtattg atggtctgac ttgccgacgc catcgcactt tgcacttgtg
     7981 ccaaacctgt tgtaactgca gccgtatcct gcgccaatgc tgcctgatac gccgccgtct
     8041 gctgctgaat ggctgcctga tacgccgccg tctgagttga aaacaacgga gacaacatct
     8101 tatcggtagg ctgcgctgac ggagcagcca aatactgagg gacaccaccc cgcgcctgtt
     8161 cccgcttcgg cgcaggttca aatcggatac cgttgttttt cagacgatta atcatttcag
     8221 gagttgtctt ggtaccacgc aaaccctttg gcaatgtgcc gtcacccaaa gactcggaat
     8281 acagcaacat acctaacaaa cccgcccgtt taactaacgc tggattattc atgagcccgc
     8341 taccggcaga ttttccccat gacagcaact taccgcctgc cgctgtggca cctgctgcca
     8401 cgccgcctgc ggaagcctta ccccatccca acgctttact acctacatct ttcagaaagc
     8461 ctgcaccttt accgccaccg gcggttaata acattgcggc agacgccgct gttgccgccg
     8521 tcgtcaatgc ttgcagggct agagtggcat tagggtattc cgctgccagc tttttaaacg
     8581 aggtttccat atccaccaat gttcccgtca gactttcccg caacattgcc agcgattcct
     8641 gttgctcttg ttgcgccgct gccgacaaca ttcgcgcctc gttgttctta gcaatttttc
     8701 cgttatccaa cgttacgcca gccaacgaag ccatatattc acggatttgc gtcatatccg
     8761 ttgcagccag caaaccttgt tttgcctgca aatcaggcag cagttgcgcc aaaagcgcgc
     8821 ccttaagcat atttgcctgc tccgccgccg tcttatcgcc tgcagccgcg cgtttcttat
     8881 aatcttggta ttgcttatcc tttactagca tggcatcggc aagacgggac aacacctgca
     8941 ctgcgttttc gccgttttgc ttgccttgca caaccgagcc tatccaatcg acacctttct
     9001 tcgggtcatt cggatttgcc atcttcttca gacgacctat cgtgtcaggc gacagagttt
     9061 tactcaaaag attttgcaca ttagtcgccg cttcggcagg actgcccgat ttattcgccg
     9121 cagattgtaa gagtgagagc aggtagtcca aaccgccgac accattcatc cctgcctgtt
     9181 gcgcggcagg gagcaggctc ggcagctccc gaaccatatc ccgcacctcg aaagtgccgt
     9241 ctaaacccga ttgcaagacg tgctcaagcc cgagttgcag gtctttaccg ctcatgccgc
     9301 catctttcag ggttttaatc agttttgccg tatcctcgcc actgccttct gaggcaagtg
     9361 caaaagcata tgccgcctgc gcttcattct tggtttgggc aaaattcaga ctggtggtca
     9421 tcatgccgct gattaaatcc aaagccttat cgtgggtccc gccatttttc tcgacaagtt
     9481 caagtgccaa atccttgatc tgttgcgcac cttcagttgc aatccacgct gccgatttac
     9541 tgttatcctc aataaatgcc tgtctggaca cgcggttgat gttctcatca agctgctttc
     9601 tgttgtccat agcaggctta agcaccgtat acgctgccgc gccaccagcc accgcagctg
     9661 caccgaatct tccgattttt cccatcttgt ccgcaaatcc cgtgccctgt ttcagttccg
     9721 cgttcagctc gcgaattcgg ttacgcgtag cgaccgccgc ccgtgccaaa tcattttgtg
     9781 atgccttgcc gctgcgcgcc aaacggttaa acgcagcctg tgtcagcatg atttcgcgtt
     9841 gaatctgttt ttctgagcgg atgccggtca acgcataagt atggctttgc cgttcaagtt
     9901 gcgcccgcga tttcgcggta cgatctaatt gtcgttgagt atcagccagt agccgtctcg
     9961 ctccgtcatc tcgggcggtt aacaccaacg acagtttcat gttcccgttt gccataaaaa
    10021 gcagccttta aacgtattaa ctcaaggtca attatcgttt aaaggctgtt taagggaggc
    10081 ggtatgggct tcaaccccgt tttcgtgctt tatttgtttg ttgcgttata atactagaat
    10141 gataaccatt ttattcaaag ggatatttta tgaatatgaa aaaattgatt tccgcaattt
    10201 gtgtttcaat tgttttatca gcctgcaacc aacaatcaaa aacggcacaa gccgaagaac
    10261 ctgtccaaag tatccaggct gctgattgta ccgccccaat ggacatcaca gttgaacaat
    10321 atctcatcaa tttggagcaa gcatttaaaa ctcagaacgt ctcaacaaaa atccataata
    10381 aaaatattgt caagaccgat tgtggttatg accttacttt ggtaatggat tttggggcga
    10441 ttgcgctcaa actggacgag cagcaaaaaa ttagagctat ctcagtaggc tacattttaa
    10501 aaaccgacgg agagaaagga caaaatctag tcaataatgc cataaatgga ttacacagta
    10561 ttcaggcagt tctgtcttta actaccacag acaaattggg cgaatcggaa gcaggaaaac
    10621 aactttttac agctttaacc gaagtcgtca aagaatccaa tcagacagga gcaacagcgc
    10681 aaaaagacgt tccggcagat ggtattttat atagcgttgt ttttgaaaaa gaaacaaaca
    10741 ccattgcaat aatcggcaga aaacaaccct aaccgtcaaa taaaaaaaag gtcgtctgaa
    10801 actcagacga cctttttatt ttccctccgg ctttctcagc cgtttcgaca caatcactcc
    10861 ctcttccttc ggcgttttta tgcccaggct cgacaggatg tcttcaaacc atgccgacag
    10921 ctcggcgtgg cacatatcgc gcactgcgtc cgcccctatc cccgccttgc catcaggatg
    10981 accgcctgac ggtagctttt gtgtgccgtc aggtagctgc gttttactgc tccgccgccg
    11041 acggcgcatc ggggctcgcc gtagcggcgg acggttttag gcgcaaatcc tcctgagccg
    11101 ccatcacggt atggtagtct tcggtagaga ggttttccat cagccactgc gccgtcagga
    11161 tgtcggatgg gataccttgg gcttcaatct gctgcgacca gtaggtcagc gtctctagga
    11221 cgtcgcgttg cagcagttcg gcttttgtgg cgttttcggg cagctcgggc aagtcatcaa
    11281 tcagggcgaa cgccgccagt tcgccgccga cggtcagtgg cttgaggcgt aattcagata
    11341 cgactttgcc attgtaggtc agcccgtggg tcagtttgac ggtaatcacg cattactcct
    11401 tgattttgtg caaagcaatc atctcaatgt cgatgcgtgc ctcgttatcg acttcatatt
    11461 gctcgccggt atcgacggta aagcagtcga ggtaggaagt gcggcgatct tcgtcgttga
    11521 tggggtaaat cgtgattttc gccttggtga tgttgtccca gtcgatttcc gtaccgtcga
    11581 tcggaatggc ggcggtaacg cttaatttgt gttcggttac gccgtcacaa taaccgttga
    11641 ctcggccgtt gcggttcatc gttttgaccg gcttgcggcc tgtagtggtt tgcggcttga
    11701 tgctcacaat ctcgatatcg cggccgttca tctccataat caccgcgtcg gcataggtag
    11761 cgtcgctcat gctttatcct tttttaaggt ttcagacggc ctttaaacct gttttaaacg
    11821 ccgtctgaaa agggttacaa aatcaaatca atgcgcccgg caaagacgtg caggccgttg
    11881 accacatcgg cggggataat ggcattaaca cggttggggt cgttttgcgc acgcgccacc
    11941 accagcttgc ctttgttggc ctcggcgttt tcgatgattt cggcttggtc gagcttaatc
    12001 agcacgtcca aaatctcgct cttaaccttg ggcagcaggc ggtcgctcaa tttgtcgcgc
    12061 ggaaaacgca gggcaatgcg ctctttaacg ctgcggcgca cataatccag cgtgcggatg
    12121 gtggtaatgt cgagtagtgc cgggtcgtcg gtgttgttgg ccgacttggt ataggtggat
    12181 acggcacgca taatctgcac gcggttgttg accactgtga gcggggtcaa gccgttgtac
    12241 agcgcattgt tgcattctgc aaacagcggc cattgcgcat caggtgtaac ggccagccct
    12301 ttgatttcca gcgtgttcag cggcttggca gggtcttctt caaaggccaa caccgccgca
    12361 taacctgcgg cgatgatgcc gtttggctct accgcacctt tgtaccaagc acaggtcatg
    12421 cggccgtcgt tgatttcgcc ggtagcggtg gtggccgtgc tcaaggccgc actcataccc
    12481 aatacgccga tacagccgcg ctgctcgatg gcgttggata cgttggtaat atggttgctc
    12541 aaggctttgg cgttggcgtc atcgctaaac gggctgcaaa taatgtgata atgcttgccc
    12601 gccactttgg acaaggccgt ggcaatgtcc gcatttttgg caccgccggt aaaggcattg
    12661 gcttgataag ttaggccggt attgccggtg ctcacggtta agccgctctc gttgccgatc
    12721 tcgcctttgt gtttggccgt cagcgtaacg gctgcgccgc tgccggatgc ggtaacggtt
    12781 acatcggcgg cagtgatggc ggttttcaga cggtctgcca ctgtggcggc ggtctcgccg
    12841 gtgttaacgg ccgtgcttac ctgcttgccg ccaatcgtga tttccaccac gcccggcgcg
    12901 gtggccgtgc cggaaagggt aacggttgcg gttgcctgca cgcctgcgct gtggtcggca
    12961 ataccgataa cggtcaaatc caaataaggg ttgttggcaa atgcttggcg caccatcaaa
    13021 tgcgccagcg agccttgtcc gaacaaatcg gccgcctcgg catcgctaaa tagttgcacc
    13081 ggctctaagg cgggctgtat gcccgcggtc agcatgggtg caaccatcaa taccttttgc
    13141 ggattttgcg gcaaaccttg tacggcattg cgggtgttaa attcaatata ctgcccgggc
    13201 acgcggatgc tgcccggaat cgtgtcaaaa tcaatatggg gcatcattac tctcccttag
    13261 tattgcgggt tttggtattt ggggcggcat cctcaaccac caccaaatcg ccgtcatcaa
    13321 tcatgcggcg gtaatacagg ctgttgccgt ccacctccac cggctcttgg ccgatatatt
    13381 cgtgcgggtt gtgttcggtc ggcacacgca ggccatctgc cgccgttact ttgattttgc
    13441 tcattgctta tccttaaggg ttaggtttac aggtatttca tcggcggatt gcgggtcgta
    13501 aattttgccg tccaacccct cgaaatcagg ccacggctcg ctcaatgtac cctgatactt
    13561 ggtaaagata tggttggggt cgtcgggatt gtcggtgcgt tcggggtagc ggtcattttc
    13621 caacccgcag gtgttaaagc ggatggcata ctcaaccgca tatatgctta ctgcggcgtt
    13681 ttgcaccagc acatgattgg caatcgcgcg caccgctttg ggcaccaagc cgcggctatc
    13741 ggcaaaaccg agccgctggc cgtcaagcag gcggcgaaca gcgcggatta aatcgttgct
    13801 gccgatttcg cggctgtcga tgccgccttg ccgctgcgcc tgctcgttgc gcagattgcg
    13861 ggccgccacc atcaccacaa attcggcggt atcctgataa cgtccgcata cgccgccggt
    13921 gctggcaggc tcaactttgc tgccgccata cgttacccaa accgcaggca gcgtatggat
    13981 ttgccccgcc aaatcgtcgg cctcgccgtt gtagctttta accgtgcgca ccatgcgacc
    14041 caagccccgt ttcagacggt ctgttatcgc ttgctcaata tcagtgatca cgcccgaaca
    14101 ccttattttt accgtttaca aacacaatac cgtcttcgcc cgcggccact tcttcgccgc
    14161 tatcatccac gcccagctgc acttcgcctt tggccatagc acgcagcagg tcgagcacgt
    14221 cgattttgta gcggttgcgg atttcgtcgg taatcaacac gccctgagcc gccgtcagac
    14281 ggtagcgggc aatgtcgcag caaaggcgca ccaagatggg cggcagatcc tcaaaaggtc
    14341 gtctgaaccg ccccagatac gcgtcgattt cggcagtggc gtccaccagc gcggtttgtg
    14401 cgacctcgcg gtcaatcagc ccctcgttgt tgcggtcggt gagctgcaag acttccagct
    14461 caccgaaacg cgcaaccata tcctcaaccg tcgcgtatgc cattactcga ccgccttgcg
    14521 ttgcagcata ggctcggcgc agattgcctt ccacaccgct tcgccgactt cggcgcgctt
    14581 cacttcgcgc cagccgccgt caaacagcag gccgccgcgc caaaattctt tgccgtctgc
    14641 gccggtactg acgagcatca catcgcggct gtccgccaaa gcgtcggcgg cacgttgcgt
    14701 atgctgcact ttgagttcgg caagttcggc ggacagtgcc tttttgtcgt cttcggcttt
    14761 ttccaaggct gtggtcagca tttcgacatc gtttcgggcg gcggcaagct ctgcctgcac
    14821 ggcgtccaat tcggctttga tgtcttcaaa cgacggggcg gcggtttcgg cggtttctgg
    14881 tttgttgttg gtttttgcca tgatgactcc t
//
LOCUS       DAWHRS010000036        14880 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWHRS010000036 DAWHRS010000000
VERSION     DAWHRS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.036"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(101..3586)
                     /gene="smc"
                     /locus_tag="WIB82_000775"
     CDS             complement(101..3586)
                     /gene="smc"
                     /locus_tag="WIB82_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ3842105.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHTGQTIAKHEECKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEETLHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPQGFVPPAPLPQGQAAWL
                     SDDLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQNNLSAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELESLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLLDDISTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIAQLEQQTFDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQAQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNTSIGSLTQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3588..3839
                     /locus_tag="WIB82_000776"
     CDS             3588..3839
                     /locus_tag="WIB82_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842106.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     ALEPRFRQCPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3836..4558
                     /gene="lpxH"
                     /locus_tag="WIB82_000777"
     CDS             3836..4558
                     /gene="lpxH"
                     /locus_tag="WIB82_000777"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ3842107.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4827..6413
                     /locus_tag="WIB82_000778"
     CDS             4827..6413
                     /locus_tag="WIB82_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ3842108.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6581..6784)
                     /locus_tag="WIB82_000779"
     CDS             complement(6581..6784)
                     /locus_tag="WIB82_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ3842109.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6900..7397
                     /locus_tag="WIB82_000780"
     CDS             6900..7397
                     /locus_tag="WIB82_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ3842110.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7479..8672
                     /locus_tag="WIB82_000781"
     CDS             7479..8672
                     /locus_tag="WIB82_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ3842111.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVGLLKAKG
                     ASQNFDFIIKQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9075..10010)
                     /gene="hemC"
                     /locus_tag="WIB82_000782"
     CDS             complement(9075..10010)
                     /gene="hemC"
                     /locus_tag="WIB82_000782"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ3842112.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESESLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10129..10644
                     /gene="ybeY"
                     /locus_tag="WIB82_000783"
     CDS             10129..10644
                     /gene="ybeY"
                     /locus_tag="WIB82_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ3842113.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10646..11470
                     /locus_tag="WIB82_000784"
     CDS             10646..11470
                     /locus_tag="WIB82_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ3842114.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11599..12978)
                     /gene="nhaC"
                     /locus_tag="WIB82_000785"
     CDS             complement(11599..12978)
                     /gene="nhaC"
                     /locus_tag="WIB82_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ3842115.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13269..14492)
                     /locus_tag="WIB82_000786"
     CDS             complement(13269..14492)
                     /locus_tag="WIB82_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ3842116.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3509 a         4194 c         3821 g         3356 t
ORIGIN      
        1 caacgctgta ccgatttaaa tttaattcac tataccgtct gaaaacccgt atcgcaacac
       61 ttcattaaaa acggcaagat tcagccgttc tgcaacccgt tcaaacggct tccgccattt
      121 ctaacgcctg tttgatgtcc acggcgacga cgcgcgagac acctttttcc tgcatggtta
      181 cgccgaccag ctgctccgcc atttccatgg tcaggcggtt gtgggagatg tagaggaact
      241 gggtttgcgc cgacatttct ttgaccaggt tgcagaagcg cgaggtgttg gcgtcgtcca
      301 gcggggcatc gacttcgtcc aaaaggcaga acggagcggg gttgaggctg aacagagcga
      361 acacgaggct catggcggtg agggcttttt cgccgccgga gaggaggtgg atggtgctgt
      421 ttttcttgcc gggcgggcgc gccatgatag acacgccggc ggtcaaaagg tcgtcgccta
      481 tcattttgag ggtggcttcg ccgccgccga acagggtcgg gaagaaggtt tggactttgc
      541 cgttgacggc atcgaaggtt tctttgaaac gcgctttggt tttgtcgtcg atttgggcga
      601 tggcttcttc caaaatggtg atggcggcct gcacgtcttc gctttggctg cggtagtagc
      661 cgtcgcgttc gcgcgcttct tcgagttctt gcagggcggc gaggttgacg gcgccgaggg
      721 cttcgatttg ctgggtaagg ctgccgatgc tggtgttcaa tactttcggc gattctttcg
      781 ccaacgcttc gagcgcgtcc aaatcggcgg cgcgttcggt caagttttga tggtagcgtt
      841 tggcgttaat cagggcttcc tgctgctgca acagggcggt ttgggtagcg gcctgaagct
      901 gtggcaactg ggtttgcagg gtttgtacgc gggcgtattg ctccctgcct tgcgcctgaa
      961 tctgtgcgag tttttcttgt acgacgatgt attcttcgtc caaggtctgt acggcttcgc
     1021 ttaattcttc aagcttgatg tgctgctcgt cgttttggaa ctcggtttca taggcaaggg
     1081 caagctcttg ctggcgttcc tgccagtcga aggtttgctg ttcaagctgg gcgatttgct
     1141 gttggtagtt ttgtttttgc tggttgagct tgtggacggc gacttcggca agcccgtatt
     1201 ggcggttggc ttccaacagg gcaagctgcg cctgtttcag acggccttgc tgctcttggc
     1261 ggctgtgtgc ggtggtttgc tgttggtgtt caagttcggc ggcggcttcc tgcaaagtag
     1321 agatgtcgtc taaaaggccg tcggacgtgt gttgcaacac ggtctgttct tccgccaact
     1381 gcgccagttc gcgctcgatg tgttcgcggc ggatttgccc ttggttggta cgcgccagaa
     1441 gttcggcggc acgttgctgc gcttggctgt attggcgcgt gtgctgctgt tgttgctgca
     1501 tcaggttttt gtgctgcact tcggacgagc gcacggcggc ttccgcctgt ttgaacgcgg
     1561 cttcggcggc agaaagttcg ggggcgaggc tttccagttc ggaagcgatg ccgtcgaggc
     1621 gcgctttttg ggcaatcagg ctttcctgcg cgggtttggc atagagcagg acgctgactt
     1681 tgtcgacctg atggccttca ggcgtgagcc agatttggtg tgcgctcaaa ttgttttgat
     1741 gcgcgagggc gtagctcaaa tcgggtgcgc acaatacgcc gtcgagccag tggtgcaatg
     1801 ccgtctgaaa cggcggctgc gcttggattt ggttcagcaa tgcctgtacg ggcagggatt
     1861 ttttgatgcc gccggagagg tcgtctgaaa gccacgccgc ctgcccttgc ggcaatggcg
     1921 cgggcggaac gaaaccttgc ggcacgctac gggcgtgcag gcgttcggca aggatgacgg
     1981 acaaggcgtg ctgccactcg gcgggcgcgg tgatgtgttg ccacagttgc ggcgcggcgg
     2041 cgtggtcggt tgcctgccag aagtcggcgg cttcctgctg ttgcgacagg atttgcgaca
     2101 acgcctgctg ctgcgcctgc aaggtgatgt gttgctgctt caggctttgg aagcggtttg
     2161 aggccgtctg aaacgcctcg cgggcggcgt gtaaggtttc ttcggcggca atgatttgtt
     2221 cttcgtaatg ctcttgctgg ctttgcaaca aggcggcggc ttcctgcgcg gcggcggttt
     2281 ccgcttcgtc gggcaggttc agggcttggt tttcctgttt cagacggcct ttgcactctt
     2341 cgtgcttggc aatcgtttgt ccggtatggg caagctgctg ctgcttcaac gccagttcgc
     2401 ggcggatgcg gtttgcctcg tcctgctggg tttggaaggc ggcgttgagc gtggcttggg
     2461 cttcttccaa ttcgggcaga cgctcttcgt gttcggcaac ctgcatcgcc cactccgcca
     2521 gctcggtttg cttttcttcg acgtataact cgttttcttc aagctgcacg cggatttgct
     2581 gctgctcttg atgaatacgc tgcaactgcg cctgtgccgc ctgcttgtcg cgttcgatgc
     2641 gttgatgcag gttttgccgg tggcggattt gttcttccaa acgggcaatc tgctcgcgca
     2701 acacgccgcg tttgttgccc aattcgtgta ccgcctgctg ctgcgactgt tcggcggtct
     2761 gcaaggcatg tacttcgtcg tttaacgcct gaacctgcgc ggcggtttcg tcctgctgcg
     2821 cctgcaaaga ctgatgctgc gcggtcgctt tgtcggcggc ggcaagcgat tgctgccatt
     2881 gggcgtaatc gagcaaatcc tgttgttggt tcaactgcgc ggtcagggat ttgtagcgtt
     2941 cggcagtttc ggcttgtttt tccagctttt ccacctgacg cgccaactcg ttctgcaaat
     3001 cgcccaaacg ctgcaaatgc tcgcgcgtgt ctttcagacg accctccgtc tccttgcggc
     3061 gttccttata tttggacacg cccgccgcct cctcgatata agcgcgcaac tcctccggcc
     3121 gcgcttcgat gatgcgcgaa atcatcccct gctcgataac ggcataaccg cgcgcgccca
     3181 cgcccgtacc cagaaacaaa tcagtaatgt cgcggcggcg cacggtctga ttgttgatga
     3241 aatatgtcga ttcgccctga cgcgtcaact gccgcttgat gctcacctcg gcatactgcc
     3301 cccacgcccc ctgcaaactg tggtcgctgt tgtcaaacac cagctccacc gaagccctcg
     3361 gcgcaggacg gcgcgtcgcc gcaccgttaa aaatcacgtc ctgcatactc tcgccacgaa
     3421 gctgcttcgc cgaagcctcg cccaacaccc agcgcaccgc gtcaatcaca ttcgacttgc
     3481 cgcagccgtt cggcccgata accgcaacaa gctgccccgg aacatgaatc gtggtcgggt
     3541 cggtaaaaga tttgaagccg gagagtttga tgtgggtcag gcgcataatg gtcggaaaaa
     3601 ataaaaaaga agcgtatttt aacggaaatc ccgccgcacc accccatatc ttgccggcaa
     3661 aaccttaccg catcccgcac ccgcgatgcc gtctgaagcc ttcagacggc atttttaacg
     3721 cgctcgaacc ccggttccgc caatgcccga tgcttggcgc ggcatttgcc gtattgcccc
     3781 gccgccgggc gcgttataat ccgccacgac atccgcccga tttggaaacc tgcctatgaa
     3841 acccgcctat ttcatctccg acctgcattt gagcgaaaag caccccgaac tgaccgcgct
     3901 gctgctgcgt tttttgcgtt cttccgccgc cgggcaggcg cgggcggttt atattttggg
     3961 cgatttgttt gatttttggg tgggcgatga cgaagtttcc gagttgaata cttcggttgc
     4021 gcgtgaaatc aggaaattgt ccgacaaagg cgttgccgtg ttcttcgtca ggggcaaccg
     4081 cgacttcctg atcggtcagg atttttgccg gcaggcgggt atgacgctgc tgccggatta
     4141 ctcggttttg gacttgttcg gctgcaaaac cctcatctgc cacggcgaca ctctgtgtac
     4201 ggatgacagg gcatacctgc gtttccgccg catcgtgcat tgcaggcggc tgcaaaaact
     4261 gttcctgatg ctgcccctga aatggcgcac gcgccttgcc gccaaaatca ggcgtgtcag
     4321 caaaatggaa aaacaggtca agcccgccga tattatggat gtcaatgccg cctttaccgc
     4381 gcggcaggtt cgcgccttta acgcggaaag gctgatacac gggcacaccc accgcgagca
     4441 tatccatcac gaaaacggct ttacccgcat cgttttgggc gactggcata acgactatgc
     4501 ttcaatcctc cgcgtagacg gggacggcgc ggtattcgtg ccgctggaaa aatgctgaaa
     4561 atgccgtctc aagcctttcg ggcggctttt tttacacgcc cctttcccac actccctccg
     4621 ccgcatcatc accatcctta acccgcatta aggggcgtta tcgataaaat gcttgaccga
     4681 ttacataaga ttacgtaaag tgtgcaaacg cataacaatt ggtcttacca atttatcttt
     4741 taaccaaaat atcgggtgta cgggatttga acgtctttcc aaacctcgac gcgccttatg
     4801 atgcaaatgc ctaaaaagga ttcgttatgg cacttttcct cagcatattc cccatcgtcc
     4861 tgctgatttg gctgatggtg aaaaaaaaca gtatgccctc ctacgtcgcg ctgccgatta
     4921 ccgcagtgct gatttacgcc atcaaacttt tctacttcga cgatgcgggc atgctgctca
     4981 acgccaccgc cgcttccggc ctcgtcaaaa cgctcacgcc gattaccgtg attttcggcg
     5041 cgattatgtt caaccgtatg atggaaacca cgggctgcat cgatgtcatc cgcaaatggc
     5101 tggcgaccat cagccccaac cccgtagcgc aactgatgat tatcggctgg gcttttgcct
     5161 ttatgattga aggcgcatcc ggcttcggca cgcctgccgc gattgccgcg ccgattctga
     5221 tgagcttggg cttcaacccg ttaaaagtgg cgattttcac tttggtgatg aactccgtcc
     5281 ccgtctcttt cggcgcggtc ggtacgccga cctggttcgg tttcgcaccg ctgaacctga
     5341 gtgccgaaga catcctcgcc atcggcaggc agaccggcgt aatgcacttc ttcgcaggtt
     5401 tcgtcatccc cgtcatcggc ttgggcttca tcgtaccttg gtctgaaatc cgcaaaaact
     5461 tgggcttcgt cgccattgcc gtcttctcct gcaccattcc ttatgtcgca ttggcgatgg
     5521 tcaacgaaga attcccgtcg ctcgtcgccg gcgcaatcgg cctgatggtg tccgtattcg
     5581 ccgccaacca aggctggggc ttgagcaaag accacgccaa agacccgaat gccgaaaaag
     5641 tgccgttcgc ccaagtcgcc aaagcactcg cccctttggg tatgctgatc ggcatgctgg
     5701 tggttacgcg catcaaacag ctcggcatca aaggcatttt gaccagcaaa gaagaatggt
     5761 tcagcttcca actgccgttt gatttgtcca aaatcaccgt cagcgactcc ctgacgatta
     5821 ccttcggcaa tattttcgga caagatgtca gcgcgtctta ccaaacgctg tacgtcccgg
     5881 cttggattcc gtttgtgctg accgtttgga tttgcatcct gctgtataaa accaaattca
     5941 aagatgcctg gacgatttat tccgtaactt tcaatcaaac caaaaaaccg ctgcttgccc
     6001 tgatgggcgc gctgattatg gttcagctga tgctggtcgg cggcgacaat tcgatggtga
     6061 aaatcatcgg taaggaattt gccgcaatgg cgggcgaaca ctgggtttat ttctcgccgt
     6121 atctgggcgc gatcggtgcg ttcttctccg gctccaacac cgtgtccaac ctgaccttcg
     6181 gaccgattca gcagcaaatc gccttggata ccggcctgtc cgttaccctg attctggcgt
     6241 tgcagtccgt cggcggcgcg atgggcaata tggtgtgcct caacaacatc atcgccgtat
     6301 gtaccgtatt ggatgtgaaa aattccgaag gtgcgattat caagaaaacc gttatcccga
     6361 tggcgattta cggcgtgatt gccgtcgtcg cggcaatgat tttcttcctc taggcgaaac
     6421 gccaccatcc atagcggcag cacgcgggga acggaaaccg ccgttccccg tccgggcggc
     6481 gcgtcgggca gaatctcggt gccggaacag cccgccccgc caaacaaatg ccgtctgaaa
     6541 ccggaaaagg cttcagacgg catttttccg ccgcttgcgt tcaggcgtaa tgatgatgcg
     6601 gcatcttgcc gtcaaggcgg tagcgcgtgc cgcaatacgg gcaggcaacg ctgcccgatt
     6661 ctccttcgca caaaggcaaa aacactctcg gatgcccgtt ccactgctcg ttgtcgggtc
     6721 ccgagcaata cagcggcaga ttttccggca acacggaaat ttcctgcaga ttcagattgt
     6781 ccatttgatt ttcctttgcg tggttggcgt gcgcccattt tacgccatcg gcaggctaaa
     6841 ggatattttc ggcgcaaagc cgcaatccgc tataatcccc acttttcaga cggcatacca
     6901 tgactgcgct tacccttccg gaagacatcc gccaacaaga gccatccgcc ctgctctata
     6961 ccctcgtttc cgcctacctc gaacacaccg cccaaaccgg cgacgaatcc ctctcctgcc
     7021 tgtccgacga ccagcacacg ctgaccgcat tctgctacct cgacagccaa gtcgaagaag
     7081 gcggcttcgt gcaactcatc gcctccggtt acggcgaata tattttccgc aacccgcttg
     7141 ccgacagcct gcgccgctgg aaaatcaaag ccgtgccgaa agtcttggac aaagccaaag
     7201 ccctctacga acaacacggc aaaaccatcg aaacgctcgc cgacggaggc gcagacatcg
     7261 aatccctgcg caaacagttc cccgactttg aagaatggga cggcgcatac tacgaagccg
     7321 ccgaacaaga cctgcccctg cttgcagaac acatacagtc aaactgggaa accttcgccc
     7381 atatcgggca ggcgtgattg cgtctgtttc cagcccgtgt aaaacagcgt aaaatcggca
     7441 aacccgaatg accttccgtc cccatcaagg agcaagctat gttcttcaag cacatcgaag
     7501 ccgcccccgc cgatccgatt ctcggtttgg gcgaagcgtt caaagccgaa acccgccccg
     7561 aaaaagtcaa cctcggcatc ggcgtttata aagacgcatc cggcgcgaca cccctcgtca
     7621 aagccgtcaa agaagccgaa aaacgcctgt tggaaagcga aaccaccaaa aactacctca
     7681 ccatcgacgg cgttgccgac tacaacgcgc aaacccaaat cctgctgttc ggcaaagacc
     7741 acgaaatcat cgccagccgt cgcgccaaaa cagcgcaaag cctcggcggt acgggcgcat
     7801 tgcgtattgc ggccgagttt gccaaacgcc agttgaacgc gcaaaccatc tggatttcca
     7861 atccgacttg gcccaaccac aacgccatcg ccaaagcggt cggtatccaa gacaaacctt
     7921 atcgttacta tgatgccgcc aaacacggtt tggattggga cggcatgatt gaggacttga
     7981 gccaagcgca aaaaggcgac atcgtcctgc tgcacggctg ctgccacaat cctaccggca
     8041 tcgaccctac gcccgaacaa tgggaaactt tggcaaaact ttctgccgaa aaaggctggt
     8101 tgccgctgtt tgactttgcc taccaaggct tcggcaatgg tttggaagaa gatgcctacg
     8161 gcttgcgcgt gttcttgaaa cacaatacag aattgctgat tgccagctct tattccaaaa
     8221 acttcggtat gtacaacgag cgcgtcggcg cgttcacttt ggtggccgaa gatgaagaaa
     8281 cagcagcccg cgcccacagc caagtcaaaa ccatcatccg taccttgtat tccaacccgg
     8341 cttcacacgg tgcgaacacc attgcgctgg tgttgaaaaa tgatgatttg aaagcacaat
     8401 ggattgccga actcgatgaa atgcgcggcc gcatcaaagc catgcgccaa aaatttgtcg
     8461 ggttgctcaa agccaaaggt gcaagccaaa actttgattt cattatcaaa caaaacggta
     8521 tgttctcttt cagcggcttg actcccgaac aagtcgaccg cctgaaaaac gagtttgcca
     8581 tttatgccgt ccgctccggc cgcatcaacg tcgccggcat taccgacgac aacatcgatt
     8641 atctgtgcga aagcatcgtg aaagtcttgt aaaacaaaag cgcatacgaa atgccgtctg
     8701 aaccttaacc agttcagacg gcatctgttt tttgttaaaa aacaataaac tataacaatt
     8761 tcaaacaagc taaaaacatc tataaaatca acccaatatg atgaactcaa ataaaaaata
     8821 cgaatatcat cattctcaaa ttcaaaattt gaacgccaca gtattatccg aagccacaaa
     8881 aacctttccg ccgtcattcc cgcgcaggcg ggaatccggg aacccaatgc tgcaagaatt
     8941 gattagaaac aactgaaacc gagcaaactg gattcccgcc tgcgcgggaa tgacgaaata
     9001 aggcaagtaa aactgatgaa acaaattgat taaaaacaat aacttatatt attttattta
     9061 aattttcgat aaaatcaatt ttccgtattc aatactgctc caatcaattc ccgcgcaccg
     9121 tcgtccgcca atttcttagc gacagcgcgt ccgagcgcgt cggcatattc ggcaggggct
     9181 tgcgcgtccg cccgcaacac aaccgaaccg tcgggatgtc cgaccagtcc gcgcaaggtc
     9241 agcaagccgt tttcttctgt gcaatatgcg gccaaaggca cttggcagct tccgcccaaa
     9301 gcgcgtgcga gggcgcgttc ggcggtaacg caggcattgg taacgccgtg gttcaagggt
     9361 ttcaaaactt catacaaatc ttcgcggtgc gcggcaattt cgatacccaa tgcgccttgt
     9421 ccggcggcag gcaggctctc ggattccgac aaaatcatgc ggatgcgtcc gtccaattct
     9481 aaacgctgca aaccggcggc ggccaagata attgcatcgt attcgccgtt atcgagtttg
     9541 gacaaacggg tttgcacatt gccgcgcaaa ggtttgataa gcaaatgcgg atagcgcgca
     9601 cgcaactggg cttcgcggcg caggctggat gtgccaacaa ccgcgccttc gggcatttct
     9661 tccaaacgcg tgtattggtt ggacacaaat gcgtcaaacg gattggcgcg ttcgccgatg
     9721 gccgcaagcg cgaaaccttc aggcaaatcc atcggtacgt ctttaatcga atgcaccgcc
     9781 aaatcggcgc gcccgtcata taaagcctgt tccaactctt tgacaaacaa gcctttaccg
     9841 ccgacttttg acaaagtttt gtccaaaatc tgatcgccgc gcgtggtcat gcccaaaatc
     9901 tcgacttcgc aatcgggata cagagccttc agacggcctt ggatatgctt tgcctgccac
     9961 atagcaagca ggctttcgcg gctggcgatg acgagttttt tcgggttcat gttcaatgcg
    10021 ttcaaagaat ggaaacggcg tgaagtctat catattttga acgtttcttc agacgacacc
    10081 gtcaggtcta tgttatattt acacccacaa aacacgaaac ccgccacaat gaaacgcacc
    10141 aaaaaatacc ctttcttaac gttgcagcgg caacgtttcc atttgaactt tgaaaacgcc
    10201 tcttccgccg ccggcatccc agccgaacgc gatttctacc gctgggcgtg gtctgccttg
    10261 aaaaatgaat accgccgcgc cgacatcagc ctgattcttc tggacgaaga agaagcccga
    10321 gcctacaacc gcgactaccg cggcaaagat tacgccacca atgtattgag tttcgcgctc
    10381 aacgaaggcg aaatcctgcc ctgccaagtt tcggaaaaac tgtacggcga tttgattatc
    10441 tgcccgcaag tcgttttaaa agaagccgcc gaacagggca aaacgcccga acggcatttt
    10501 gcccacctga ccatacacgg tactttacac ctgatgggct acgaccacat cgaagacgac
    10561 gaagccgaaa taatggaagc cgaagaaatc cgcctgatgc tggcggcagg cttccccaac
    10621 ccctaccaag aggacgaata ttaaaatgga cggtacgcag tcgaaaccca agtttttcga
    10681 acgcctgatc tcccgacttg ccggcgagcc cgactccgcc gaagacgtat tgaccctgct
    10741 gcgccaagcg cacgagcagg aagtttttga tgcggatacg cttttaaggt tggaaaaagt
    10801 cctcgatttt tccgatttgg aagtgcgcga tgccatgatt acgcgcagcc gtatgaacgt
    10861 tttaaaagaa aacgacagca tcgagcgcat caccgcctac gttatcgata ccgcccattc
    10921 gcgcttcccc gtcatcggcg aagacaaaga cgaagttttg ggcattttgc acgccaaaga
    10981 cctgctcaaa tatatgttta accccgagca gttccacctc aaatccattc tccgccccgc
    11041 cgtcttcgtc cccgaaggca aatcgctgac cgccctttta aaagagttcc gcgaacagcg
    11101 caaccatatg gcgattgtca tcgacgaata cggcggcaca tccggcttgg tcacctttga
    11161 agacatcatc gagcaaatcg tcggcgaaat cgaagacgag tttgacgaag acgatagcgc
    11221 cgacaatatc catgccgttt cttccgaacg ctggcgcatc catgcagcta ccgaaatcga
    11281 agacatcaac accttcttcg gcacggaata cagcagcgaa gaagccgaca ccattggcgg
    11341 cctggtcatt caagagttgg gacatctgcc cgtgcgcggc gaaaaagtcc ttatcggcgg
    11401 tttgcagttc accgtcgcac gcgccgacaa ccgccgcctg catacgctga tggcgacccg
    11461 cgtgaagtaa gcaccgccgt ttctgcacag tttaggatga cggtacgggc gttttctgtt
    11521 tcaatccgcc ccatccgcca aacataaaaa tgccgtctga aaccgttgca agtttcagac
    11581 ggcatttcgc ttatccgctt attttttgct caaagtcagc cccgtccaac cgaataacag
    11641 ggtcaaagcc aaactcaaat agcagaaaaa ggcatacggc agatattccc aaaccggcac
    11701 gcccagcgcg tggctgatga acacgccgca tacgctccac ggtacgagcg ggttaatcac
    11761 cgtccccgca tcttccagcg tccgcgacag attgcgggaa tgtagtccga gcctgtcgta
    11821 aacgggttta aacgtttctc cagaaagcag gatgctcaaa tattgttcgc caatcaggaa
    11881 attaaccccg accgaagtca tggcaacgct gaacgtcgcg cgtccggcat tcgtcaagaa
    11941 ggtacggatg gcctccaaca gggaaggaat cacaccgagc gcaaacaaca gtccgcccaa
    12001 actcatcccg agaatcacga tggtttgcgt gaaaaacata ctttccaaac cgccgcgcga
    12061 aatcagtttg acaacatctt taaacgcttc gccttcgagt ttgtagccgc cgtaaaacca
    12121 cgcaccgagc tgacgcagat cgggcgtgct gtgcagatac gttacagcaa cggcaaccat
    12181 gacggtaaag agcatggcga cgacggcgtt gatgcgcatc aatgccaaaa tgaccaacag
    12241 cgcaaacgga atcagcgaat agccgtgcac caatcccgtg gcttcaagct ggctgcggaa
    12301 ggattcgacg ctgttcaaat cctgcgcggc gacattcggc aaaagccaaa gcatcagtgc
    12361 cgcactaatg agccacgcgg ggatggtggt gtacatcata tttttgatgt gctcaaacaa
    12421 gtcgatgccg acgatggacg cggaaatacc cgtcgtatcc gaaagcgggg acattttgtc
    12481 gccaaaaaat gcgcccgaaa caatcgcgcc cgccgtcatc gccatatcgg cctgaaacgc
    12541 cgccgccatc cccataaagg caacgccgac agtggcgcag gtggtcaggc tgctgccgat
    12601 ggacacgccg atgacggaac acagcgcgaa ggcggaaaaa taaaaataag tcggggaaat
    12661 cagtccgaaa ccgtaataca tcagcgtcgg aatcgcgccg ctcatcatca gcgcgctgac
    12721 catcagcccg atgaagaaaa acaggtaaat cgcgcccata ccctgattca acgcgcctat
    12781 catgccctgc tgcatatcgt tgtatttcaa accgcgcgcc aagccgtaca aaatcagcac
    12841 gacgatggcg gcaataatgg acatatgcgg cagccactcc aatgaaatga tggtatagcc
    12901 catcgcggca atcagagcga cgacgacggc aagtgcctca ccgcgcggca tatcgagtaa
    12961 ggatttgaaa gcgaacatga ttctttgccc gatttgatat aaagttaacg gattctaaca
    13021 caccataccg aatccgcccg acgttttcag acggcatttt agcgttttac aaaagccgga
    13081 cagaaacaaa acggcaattt tctcaattta aattaatttt tttgaaatat aattttttat
    13141 ttttttgttt gttgacatat aagactttta tggattgaag tattaaaacc tcggcacaca
    13201 cgccgcaaac accggtacgc aaaaatgccg tctgaaagat gttcagacga catttctcaa
    13261 gaaaaagatt atttgtccgc cctatatccg tacaccgcaa aaaacacgat atacagataa
    13321 cagatgacgg aaacgacaaa cgaagacatc agggtgtaag tatctgccac ccagccctgc
    13381 acgacaggaa cgaccgcacc gccgacaatc gcggtacaca gtacaccgga agccgcgttg
    13441 gtaaattttc ccaatccttt ggttgccaaa gagaaaatcg tcgggaacat aatcgagttg
    13501 aaaaaaccga tggcaagcag cgaccacatc gccacatccg cattgccgct acccgtcgcc
    13561 atcgcgacgg caagcagtac gaccgcagcc gatgcgttaa acgccaaata acggttgggc
    13621 gcgaatttcg ccatcaccgc cgaaccgagg aaacgtccga ccatcgcgcc gccccaatag
    13681 aacgacaggt aatgcgcggc agaagcatga tccagccctt tcagataacc caatacgttg
    13741 accatcaacg aaccgataga cacctccgcg ccgacatagc agaaaatgcc tgccgtaccg
    13801 aacacgagat gtttgtattg ccatacgctg gttttgccgt cgtggttgtg cgcgctttcc
    13861 tcggcggcaa ttttgcgcgc gtcgggcagc cggatcattt tcacgaaaac ggcaaggata
    13921 atcagcagcc ccgccagtcc caaataggga atctgtacgg aagaaatctg ttccgccttg
    13981 ctgacggttt gggttgcgtc cgccagaatc aggaacgcgc cgatttgcgg cgcaatggtc
    14041 gtacccaaag cgttaaacgc ctgaaccagc gtcagtgttg ccgattcctt gccgggtttc
    14101 gccagcaggg taacataagg attaccggcg acctgaagca gcgttacgcc ggaagccaaa
    14161 ataaacaacg cgcccaaaaa taccgcgtaa gaatggctac ccgcagcagg ataaaacagc
    14221 aggcatccga ccgccgtcag cagaaagccg ccgataacgc cgtttttgta gccgactttg
    14281 ccgacaaaag cccccatcgg gatggacatc accgcatagg cggtaaagaa acagaattgg
    14341 atcagcatcg cctgaacgta agacaggtcg aaaatttctt tcaaatgagg gataaggatg
    14401 tcgttcatgc aggtaataaa acccatcatg aagaacagcg tggtcaagac gaccaatggg
    14461 gacgtatgat tgttttgtga ttgcgcagac atgatttgcc tttcgtggtg tcggaatatt
    14521 acccgtttca agaaaataga aattgacatt tcttaacggc aatgcaaatc atactgatat
    14581 ttgaacattt tgcaaacttt atcggatgca ggcaatgcgc cgatctgaaa ttcaagcagc
    14641 agaaattaat attttacagt tgcttgcata gattttaata gattttaaaa aattccggcg
    14701 gacttgcaaa aaccgttccc aataaaatga aaatccgccg ccgtatccgg aaaattcgga
    14761 aatgccgcat cgtggcatca aaaccggagc ggaaagaatg tcgtatgccc gacaaaaaag
    14821 gggcatacgg ctgtgccgta ttttttgcac cgtcctccgc ccttcggcgg gggcgtgaaa
//
LOCUS       DAWHRS010000037        14733 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWHRS010000037 DAWHRS010000000
VERSION     DAWHRS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.037"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(4..762)
                     /locus_tag="WIB82_000787"
     CDS             complement(4..762)
                     /locus_tag="WIB82_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ3842117.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NHWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(787..1677)
                     /locus_tag="WIB82_000788"
     CDS             complement(787..1677)
                     /locus_tag="WIB82_000788"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ3842118.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            complement(1697..2263)
                     /locus_tag="WIB82_000789"
     CDS             complement(1697..2263)
                     /locus_tag="WIB82_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ3842119.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            complement(2314..3111)
                     /locus_tag="WIB82_000790"
     CDS             complement(2314..3111)
                     /locus_tag="WIB82_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ3842120.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAVKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVGRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKN
                     IIEPRHPTRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            complement(3166..3816)
                     /locus_tag="WIB82_000791"
     CDS             complement(3166..3816)
                     /locus_tag="WIB82_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ3842121.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            complement(3828..4868)
                     /gene="murB"
                     /locus_tag="WIB82_000792"
     CDS             complement(3828..4868)
                     /gene="murB"
                     /locus_tag="WIB82_000792"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ3842122.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLCDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVELANADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIEQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            complement(4943..6322)
                     /gene="norM"
                     /locus_tag="WIB82_000793"
     CDS             complement(4943..6322)
                     /gene="norM"
                     /locus_tag="WIB82_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ3842123.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGTVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            6657..7808
                     /locus_tag="WIB82_000794"
     CDS             6657..7808
                     /locus_tag="WIB82_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ3842124.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVVDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            7912..9210
                     /locus_tag="WIB82_000795"
     CDS             7912..9210
                     /locus_tag="WIB82_000795"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ3842125.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGVGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            9621..10058
                     /locus_tag="WIB82_000796"
     CDS             9621..10058
                     /locus_tag="WIB82_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3842126.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            10072..10482
                     /locus_tag="WIB82_000797"
     CDS             10072..10482
                     /locus_tag="WIB82_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3842127.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            10599..10991
                     /locus_tag="WIB82_000798"
     CDS             10599..10991
                     /locus_tag="WIB82_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3842128.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            11199..11591
                     /locus_tag="WIB82_000799"
     CDS             11199..11591
                     /locus_tag="WIB82_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3842129.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            11705..12265
                     /locus_tag="WIB82_000800"
     CDS             11705..12265
                     /locus_tag="WIB82_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842130.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(12566..13405)
                     /gene="htpX"
                     /locus_tag="WIB82_000801"
     CDS             complement(12566..13405)
                     /gene="htpX"
                     /locus_tag="WIB82_000801"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ3842131.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            13621..14268
                     /gene="adk"
                     /locus_tag="WIB82_000802"
     CDS             13621..14268
                     /gene="adk"
                     /locus_tag="WIB82_000802"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ3842132.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
BASE COUNT         3463 a         3656 c         4148 g         3466 t
ORIGIN      
        1 tatttaaaac gattcgccgg gagcgagata acgccatttg ccgggcggca gcctgccgag
       61 tttgaccttg cccatgcgga tgcgtttcag cccgacgacg cgcagtccga ccagttcgca
      121 catacggcgg atttgccgct ttttaccctg tttcaacacg aagcgcagtt ggtcttcgtt
      181 ttgccattct acttgggcgg gacgcagttt ctcgccgtcc aaactcaatc cgtgattcag
      241 taaggcaagt cctttttcgt ccaatttgcc gcgcacgcgc accaaatatt ctttttcact
      301 gccgctgttt tcgccgataa gctgcttggc gatacggccg tcctgagtca ataccagcaa
      361 tccgaccgag tcgatgtcca gcctgccggc gggggcgagg ccgattttgt gtttcggatc
      421 gaaacggatg cggccggtat cgccttccca gtgattttca ggggtaatca gttcggcggc
      481 ggatttatag cctttttccg cttgtgcgct gacatagccg acgggtttgt tcaacaggat
      541 ggtaatgcgt gccgcctgct gttcgtgggc tttcttgttc agttcgatac ggtctgccgg
      601 tgaaactttc tgaccgagta cggcggtttt gccgttgacc gttacccaac cctgttcgat
      661 atagccgtcg gcttcgcggc gtgaacaaag ccccagttgc gccatgcgtt tggagaggcg
      721 cacggcatct tctgtatggt cggaggaaat tttgggattc atggatactt tcgggtaaag
      781 gccggcttag actaattgct cgccccagtg cagtttttgg cgcagggttt tgaaatattg
      841 gtagtcggtc gggtgcaaaa tgcgtaaggg atttcggtag cggcggatgg tgatgcggtc
      901 gaggttttgc acgtcgatat gggtttgacc gtcgaaatgg acgcgcgcgt cgccgccttg
      961 ggtaacgagg atttcgattt cggacgtgtc tggaatggcg atggggcggt tggtcatgga
     1021 ttgtgggcag atggggacga gcgtgaaggc gtgtaatcct gcctgcatga tggggccgcc
     1081 ggcggcaagc gaataggcgg tcgatccggt gggggtggag acaatcagcc cgtccgaacg
     1141 ctgggtatag acgaattccc gattgacgaa gacttcaaac tcaatcatct gtccggcacc
     1201 gccacgggag aggacggcat cgttgagggc gatggcgcgt tcggcggttt tgccttcgcg
     1261 gatgagtgcg gcctcaatca ggatgcgctc ttcggcaagg tatttccctt ctaaaacggg
     1321 caatagcttg tccgtcatat attcgcgggg aatttgggtc aggaagccca aatgcccttg
     1381 gttgatgccg ataatcggaa cggcgcgcag ggcgatttcg cgggcgacgg agagaaaggt
     1441 gccgtctccg cctaaaacgg cgaccaggtc gcagtattgc cccagttcgg tcttgttgac
     1501 gatatggcag ccgacggtgt cttgggtata gatgcagcct tcctttatgc cgacttcgtc
     1561 gagatagacg gtaaagccgt gctgcttcaa aaaggtaatc agcgtgtgtg cggtgtcttg
     1621 gatgtcgggc gtgttggggc gggttacgat gccgatgttg tgaaaagggc tgttcatgtc
     1681 ggatgccgtc tgaaggttag tctatccaaa tgtcgcgttc gagccggtcg aggcgttcgt
     1741 tgaggcgttc cacgccgtcg cgcagtctgc ttatttcgtc gaggcagtcg gcaagggctt
     1801 cgttgccgat gtttgcggac tcggattcgc gggaaaatcc gccgatttgt tcggcgatgt
     1861 tcctgccgat ttgtttgatg ccgtgtccga tgtcggcggc acggctgccg atgtctgcct
     1921 gcgtgccgaa aatccgtgcc aattcgtccg atgcgcggga acgcaggctg ccgagcaggg
     1981 acagtaccgc gatgccgagg atgaggtcgc cttcgagccc gatgtcgccc gccccgggtt
     2041 cgcctccttg gaggattttc tgtaccgcgc tgttgcggaa ggtaatttcg gtgtctgcaa
     2101 agccgtttcc cgccgagagc aaaccgtctt ccgtgatgcg tcccgccagt ttcagcccgg
     2161 caatgttcag ggtcagtgtt ttgcctgcaa aggcggcaag ttccgagcgg ctgtccgggc
     2221 tttgcagaat caggcggttg atgatgggga ggagggcgga catattttct gatcggggcg
     2281 gagaatgcct gtttgttgct gttgccgctt attttacagg cttaagccgt tatcgcaaac
     2341 ggtacggatg attttgccca cgctgtcgtc gggtgcaaaa tccggcgcgg gcaagccgag
     2401 tttggcggct tcttccgtat agaattcgcg tcgtgtcggg tggcgcggtt cgataatgtt
     2461 tttcagccgc ctgccgccgg ggttaaatgc cgtctgaaac aggctttcga cggcgatatt
     2521 acggtggacg atgttgatgg ggcggttgcc gcccgggatg ttttgctttt gaacaaggcg
     2581 gccgacggga tggcgttcgg cgcaataaag cccgcccagc cgcaggatgt cgatgttcgg
     2641 aacgccgctg tcgagcaggt gttgttcggc ggcgaggatt tggcgggcgg actcggtttg
     2701 cggatcgggt agggcgattt cgtcgcattc gcgcgctgta tcgccgtaaa cgctggtact
     2761 gcttgtgaaa atcaggtgtt gcacgttgca cgcccgggca agttctgccc attgtttgac
     2821 ggtatcggcg taatgtgtca gcgatgatgg cggcaagagg cagaaccaaa cgggtttgtt
     2881 ggcatggtgc cgccaaaagc ttgtatctcg ggcaaggttc gcgctttgaa acgcgctgtc
     2941 ttgattgagg tcgatggtat cgaggtgtat gggcagattg atatcgtccg aagtcaggct
     3001 gcgtttgacg gcggcaacgc ggctgccgtg ttggtaaaac ttttgtgcca gcggcaggcc
     3061 gaggtaacct aggcctgtga tggagatatg gggcgggggg actgcgcgca ttcgctgata
     3121 ccgtcgggta agtgccgtct gaaggctgat tcggacgctg tgggtttacg ggttgccgtt
     3181 gccgattttc cggtcgtatt tcttgcggtg ttcgggcaaa agataaggac gaaggaggct
     3241 taaggtgcgg cggatgccgc gtgctttgga gtcttcggtc agcttggtgc ggccgcgcag
     3301 ggagctgtcg aagtcgaacc agtatttcgt gaccatccac atattgacgg cgagatcgtt
     3361 catggcggtt tggtcggctt ggatgatgtt cagaccgttg agctgggtca gcaggttgac
     3421 caagagcggg gagactttgg cttgggtgaa ggtgttgtgt tcgcccaata attcggcact
     3481 gcgggcaagc agggtgttta catcgctaaa gaggaagcgg tattcccaca tcacgtcgta
     3541 aatgcccgcc atataattga tggagtcttc cacatcagac ggcaacacgg cttcgttgag
     3601 gtattccagc agggcttcgc tgtaacgttt gaacagttgg acgatgattt cgtctttgtt
     3661 gcggaagtgg taatagagat tgcccggact gatgcccaag tgggcggcaa tatggttggt
     3721 gctgatgttg cgctcgcctt cctcgttgaa aagcgcaagg ctggcgtcga tgatgcgtgt
     3781 gtaagtattg attttggcgg ggcgggtcac gggcatactc cttggattta caggctgaac
     3841 gaagcaggca gccaattggg ttcggcgtgc aattctacct gaaaccgagc aaatactgta
     3901 aatttgatgt gttgcgccaa ctgccggaca tcgtttgccg aggcgttgtt tttgttcact
     3961 aagaccaaag cctgcctgtc atgtaccgcc gcgccgccga tttggaagcc tttcagacga
     4021 cattgctcga ttagccagcc ggcggcgagt ttgaccgaac cgtccggctg cggataatgc
     4081 ggcatatcgg gatgctgctc caacagggcg gcagcttttt ccgcgccgat gacgggattt
     4141 ttaaagaaac tgccgacatt accaagcacg ttaggattag gaagtttact gttgcggatt
     4201 gcacacactg catcggaaac atctttcgcc gtcggcaccc tgcccgcgct cagttcggca
     4261 acggcggccg ccaaatcgcc gtaacccaag ttcggcacaa aatgcgtttt taatgcaaat
     4321 acgaccgaaa caatcacata acgcccttta ccctcctgct tgaacaggct ttcgcggtag
     4381 gcgaagcggc agtcggcatt ggcaagctcg acaaaggtct ccgtatccaa atcaaagcag
     4441 cgcacgctgt gaatcacgtc tttcgcctcc acgccgtatg cgccgatgtt ctgcacgggc
     4501 gatgcgccga ccgtacccgg aatcaggctc aggttttcca aaccgctcaa acccagcgca
     4561 acggtgtgca ggacaaaatc gtgccaaatt tcgcccgcct gcgcttcaat cagaaccatg
     4621 ccgtctgaac gcgcaatctc gcgtatgcct ttgttttcca tgtgtacgac cagtccggcg
     4681 taatcctgca tcaaaaggat gttgctgccg ccgcccagcc ataaaacagt atcgcggtcg
     4741 aactcgggca gtcggacgat gtcgcacaac ccgtcggcgt gttcaagcgc aataaaggct
     4801 cgggcttggg cgcgaagacc gaaggtgttg tagggggtga ggtcggttcg gtatcggatg
     4861 ggttgcatgg tttgaacttt aaaatgggta ttgtcagtgg cgggcttcag cccatagttt
     4921 catattttaa tcggcggagt tttcagacag ccttatgcga tctgaccatc tccctactgc
     4981 acaattccaa gcaccacacc aaggcgatgg cggcgatggt gagcgaggca atcaatgccg
     5041 tccagaagcc gtaaatgccc atatcgaaac ggtaggcgag caggtagccc ggcagcagac
     5101 cgcagcccca aaaggcggcg gcgtggatga acatcggcac ctttgtaact ttgtagccgc
     5161 gcaaggcgta ggaggcgata cattgggtga agtctgccgg ttggaacaag ccggcgaaca
     5221 gtaagacggt ggcggcgatg cttaaaaccg ccggatcatt gttgtacata cttaccagcg
     5281 gcgaacggaa taataccaag gaaagcacgg taatcacggc gagcatccat cctaacacca
     5341 gtgacacgcc cgaaatataa cgcgcccgcg aaaattcgcg ccgcccaagc gaaaagccga
     5401 tgcgcaccgt ccccgccgag ccgacgcttt gcggaatcat atagagaatc cccgacaaac
     5461 tgatgccgac ctgctgcgcc gccacataat cctcgccgaa aggcgcaatc aaaaacacga
     5521 taaacgaaaa cgcgctggct tccaaaaaat aagacagccc gatgggtgcg ccgattttcc
     5581 aaatctgttt gaacaccgcc caatccggtt tgccgaattt cgccgtcagt ccgaatgggc
     5641 ggaagaaatt ttccttggcg atataaatcc acaatgccag cgcgctgaac caaaacaccg
     5701 ccatcgtcgc caggccgcag cctgcgccgc ccaaagcggg cataccgaat ttgccgtaaa
     5761 cgaaaatata gttcagcggc acgttcaaca caaacgccgc aaagctgacc aacataatca
     5821 ggcgcgggcg gttcaggctg gaggcgtagg cgtgcagtgc gcggtgtacc attgccgccg
     5881 gcatcgccaa gctggtgaac agcatatact gcgccattgt gccttccaca taatcgctca
     5941 aggtcagcca gttgcggaac ggcgtaatcg ccgcccacat caagaccgtg ccgaacacgc
     6001 ccaaaaacag cccgaaccaa atcccctgcc gccccgtttc gcccacttcg tcggttttac
     6061 ccgcgccgta aagctgggca atcatcgggt tcagcgccgc cataatgccc ataaaggtaa
     6121 tataaaccgt ggcaaacgcg ctgctgccca aagccaccgc cgccaagtct tccttgcctg
     6181 caccacccgc catcacagta tcgataaaac cgatgcccac ctgcgcgacc tgcgccaaca
     6241 gcatgggcag ggcaagagcg gtcagcaggc ggacttcttt caggaagacg gaaaaggaaa
     6301 agcggttgag gtcgagcagc ataagtgttc aatcaacaaa aatgccgtct gaagcagaaa
     6361 acggcagcag gaagtgatga gaaataatgg cgcacattat atagtaaaaa aatgccgtgc
     6421 cgtcagacgg cggatacagg gtataaaagt atattcagat tgtgtgtatt ttatggtaaa
     6481 gtttggtttt aacgacttga cggcattgag ccgtcggaca ggggctgttc ggattctgaa
     6541 tcggaaggaa gcatcgccgt tttgacagcg gcgtgatgcg ttgcggcaaa gatgccgtct
     6601 gattgcggat cgggcagcct tttgtattta catgataaaa tagaaggcag attctcatgc
     6661 agacatggca gcttccggaa catatcgccg acgtactgcc cacgaacgcg cggcagcttg
     6721 aaagcgcgag ggagcagttg ttggcactgt tccgcgtaca cggttatgaa ctggtacagc
     6781 ctccgctgat ggagtacgca cattccctgc tgacgcatat cgatgcgggg ctttccctga
     6841 aaaccatttt ggtaacggac aggctcagcg gcaggcagtt gggcatacgc gccgacatca
     6901 cgccgcaggt ggcgcgtatc gatgcccatc ttttatccgc caaccaaggg attaatcggt
     6961 tgtgttatgc cggtccggtg ttgcacgcgc agtccgacgg tctgctgaat atgcgcgaac
     7021 ccttgcaggc aggggcggaa atgtacggtt ttgccgacat ccgtggcgac atcgagctga
     7081 tagaccttat gctgaaaagc atgaaaattg ccgatatggg caaagtgctg ctttcgctgg
     7141 ggcatatcgg catatttcgc gccttgtccg atgcggcaca tttggatgcg gggcagtccg
     7201 caacgctgct tgccttgatg caggataaag ataccggggc ggtcgaagcg caggtcaagg
     7261 cttggaagct ggacggcatg tgggcaaaag cattctcgct gttgccgcgc ctgtacggcg
     7321 ggcgtgaagt gttgtccgac gcgcgcggac ggttgccgga tttgtcggcg gtcggcggcg
     7381 cgttgggcga attgcaggcg gtgtgcgacg cattccccga ttgtgaaatc catatcgact
     7441 tgtccgagct gcgtgtcgac aattaccaca cgggcttgct gtatgccgcc tatgccgccg
     7501 atttccacga cgcggtcgcg cgcggcgggc gttatgacgg attgggcgga tatttcggca
     7561 gggcgcgccc ggcaacggga ttcagtttcg acttgcgcag ctttatcggg cgtttgcccg
     7621 ccatcgaacg gcagcccgcc gtgttggtcg atgcggaaga tgccgaagcg gcgcgcgaag
     7681 cggtcgaagc cttgcgtgaa caagggcagt gtgtcgtggt tgactacggt atcggacaca
     7741 atgtttcgga agagcttgca ggccgtctga aaaagacgga cggcgtttgg caggtcgtga
     7801 aacgctaaat acccgttcat ggcggatgaa aggcaaatcg tggcggggcg caaagccgca
     7861 ccggtttggg gattcccaca ataattttta atatcgatag gttatatggc tatggctaaa
     7921 aatgttgtag taatcggcgc acagtggggc gacgagggta aaggtaaaat cgttgactgg
     7981 ctggcggaag aagccggcgg cgtggtgcgc ttccaaggcg gccacaatgc gggtcatacc
     8041 ttggttgtcg gcggcaaaaa aaccattttg cgcctgattc cgagcggcat cctgcatgaa
     8101 agtctggact gcttcatcgg ttcgggcgtt gtcgtctccc ccgaagccct gttgggcgaa
     8161 atcgacgagt tgaacgcggc aggcgtgaaa aacgtcgaag gccgtctgaa aatcgcgccg
     8221 acctgcccgc tgatcctgcc ttatcacatc gcgctcgacc aagcccgaga agcatcgcgc
     8281 ggcaaaggca aaatcggcac gaccggccgc ggcatcggcc ctgcctacga agacaaagtg
     8341 gcacgccgcg ccattcgcgc cgccgatttg ctgcatcctg aaaaactgcg tgaaaaactg
     8401 gatgccgtcc ttgcttatta caacgtccaa ctgcaacatc tgcacaatgc cgagccggtt
     8461 aaagcggaag acgtgatggc ggttatcgaa aaagtcgcgc cgcgcattgc gccgatgatt
     8521 accgacgtgt cccgcgtgtt gaacgagaaa aacaaaaacg gcgaaaaact gctgtttgaa
     8581 ggcgcgcaag gtgcgttgtt ggacatcgac tacggcactt atcccttcgt tacctcgtcc
     8641 aactgtctgg cgggcgcagc ttcggcaggc gcgggcgtag gtcctcaaat gctggattat
     8701 gttttgggca tcgtcaaagc ctataccacg cgcgtcggtt cgggcccgtt cccgaccgaa
     8761 ttgttcgacg aagtaggcgt aggtttggca gaacgcggac acgaattcgg ttcggtaacc
     8821 ggacgcgcac gccgctgcgg ctggtttgat gccgccgccc tgaaacgctc catccaaatc
     8881 aacggcattt ccggtatgtg tattactaaa ctcgatgtaa tggacggcgt tgaaaccatc
     8941 aatatctgcg tcggctatga attgcccgac ggcggcaaaa ccgacatcct gccttgcggt
     9001 tccgatgcgg tggaaacctg caagccgatt tacgaaacca tgcccggctg gcgcgaatcc
     9061 actttcggcg tgaaggacta cggcgcattg cccgaaaacg ccaaagcata tttgaaacgg
     9121 attgaagaag tctgcggcgc gccggtcgcc atcgtctcca ccggccccga ccgagaagaa
     9181 acgattgtgc tgcatcatcc gttcgcataa ggttttgcag taaaattgcc gtctgaagcc
     9241 ctaatccgcc gttggtcata aatagtaggg tatcaacatt tcgggctaca atggtacgtc
     9301 agccaatgcc aagacgtgcc agcctgattt gttgatgtgt gtactattgc ataggcggtt
     9361 aagccatgca ggagatgaaa gtgtatatgt cgcgcaaagc ccattagccg caagcgaggg
     9421 gcggggtgcg cgagagagag gggggcaggt gctaagtcat tggttatctc cttactatct
     9481 atctaactac gggtgcatta tgcgcctatg cctgttgttt gtcaaacact atacagttaa
     9541 aatgtgtaaa tatttagtaa ggacatatac ccttttcttt acatttagct ccatcggata
     9601 ccagtgcgtt cagagaaaat ttgaaaattt ctatattttg gttgtataat gctttcattt
     9661 tagaaaggtc tataatgcca aaatcactct cgctacaaga tgttcagtta aggttttcca
     9721 gtaaatttcc tgataagacg gttttgaagt tcactaaaaa ctacgaaccg gtaacaatcc
     9781 aatgcccttt gcatgggtcg gttgtttacg ggaatctaca agctgccatg aaatcctcaa
     9841 caggatgtcc tgaatgtact cgaaatcccg aaaagccctc tccaaacgcc gttgccatta
     9901 ggttggagga cacagagaca ggggaaatct acgagtttgc aagtacactg gctgccagta
     9961 aatttatcgg ttgctccaac agcactttag ctgtacgttt aagcggacgc actccgtttg
    10021 accgattgat taggtatcgt tataggttgg taaagtaagt tcacaaccac tatggcactt
    10081 acatttactc aagcagtttc taagctaacc tctaaatttc cacatttgaa tcttgtggag
    10141 ttcaatggcg ttcgttaccc gacggtaatc gtctgtccta tacacgggag ggttacttgc
    10201 tctacattca aaagtatgtt ggactctaaa agtgggtgtc caaaatgtgc atcttatggt
    10261 gtcaattccc acaaaattcc agaagataca atagataaat tatctaaaaa tacagtattg
    10321 gaggatactg taactggcga aacacttaca ttcccctcaa gagcatctgc tgcaaggtca
    10381 ttgggtataa acccagcagc tatcactgac cgtataaaag gtcgggttca cacagagact
    10441 ttacttgcag ggaggtataa ggttcacatc tgcactaaat gacgtataca ctttttaaca
    10501 gtgtataccc ctcgccacta tcaggtgctt ctcgaaaaat ttgacatttt tataaatttg
    10561 ctattaaatc catttgacaa tcaattttgg agtctcaaat ggccaaatct ttcaaccaag
    10621 cagcttctga acttactgat atattcccta atatctctct aaccggcttt gacggtgtga
    10681 attacccagt aaccgttaat tgtcctatgc acgggaacgt tcgttattcg acatttaatg
    10741 cccttataaa gtcaaagtac gggtgtcctg agtgtgctaa gatgtcaaaa acccaaacac
    10801 ctccaaatgt agggaagccc ctcctcatcc tcgacacaac gaccaacgaa acactcacgt
    10861 tcccaagcgt tactgccgca ggtgctgctt taggtgtaca tttccaacaa atcaatcaca
    10921 ggctcaaggg tcgaacgtcg cccgacaacc ttatttcaaa caggtacaaa gtgcttgggt
    10981 acgataggta aggttcacaa caccctaaat cctacgaacc aagctcgacg gacgttcaaa
    11041 tcccggttag agttaattta tgtaaagatt tagtaaagac gtataccatt tttctttaca
    11101 ttgtgcttgc gcaggatttc aggtaggtct caaaaaattt gaaaatttct atattttggt
    11161 tgtgtaatcc atttgcacat aacctatgga gacaaattat gggtaagcga atgactttcg
    11221 ataccgccaa atcacgcttt caagagaaat ttccacattt agaattgttg gagttcagtg
    11281 gcatttataa accttccagt gttagatgtc ctacgcacgg ggttgtccaa cttttgtatt
    11341 acgacacagc tataaagtca aagtatgggt gtccggagtg cgggaaactt aaaatgaagg
    11401 aaaatacgcc tccccaaaac caaaaacctg tctccatcct cgacaccgcc acaggcgaaa
    11461 cactcacgtt ccccagcgta caagctgccg ccaaagccct aaacaccccc tacggctcta
    11521 tacgaaccaa gctcgacgga cgttcaaatc ccgacaacct tgtctgtaac aggtataagg
    11581 ttctgctata atcaacctat ggaaaatatt gaagaatacg cactgctgtc tcccgaagcc
    11641 ctgctggaac gcctggatac cgttttgagt atcagaatcg gcggcaaggg ttgggaatcc
    11701 agttatgacc gccaactttg cacagacgct ggtcgaaata caggacagtc tgtacagggt
    11761 tgtgtcaacc gtccaatacg gggatgacaa cctcaagcgg ttgacagcgg acaaacggaa
    11821 gcagtatgag ttgaacttca agatttccga gggttctacg cgtgtagagt ccgactttaa
    11881 agagactttg gttcggttcg gtagagatat gcttcaagat atgcccccta aaatccgttc
    11941 ggcaacgctg gtagcgttga cgaccctgct tgtcggaggg gcgttgggtt acggttattt
    12001 ggaatacctg aagcaggttg cttcggaagg gtatcagacc gagcgtctgt ataatgccgt
    12061 cgaccgtctt gcagaatccc aagaacggat aacgtccgcc atcctgaagg gtgctagagg
    12121 tgccgatttc gtgcaaatcg gcagacgttc ctacagtagg gaggatatat cggaggcaaa
    12181 tagacgtgca gagcgtgtcc cgtatggcgc agagttggtt tcagacggca attttaccgc
    12241 tgttttatct gatatagggg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
    12301 tataaatagt acggaaccga ttcacttggt gcttcagcac cttagagaat cgttttcttt
    12361 gagctaaggc gaggcaacgc cgtactggtt tttgttaatc cactatattt tctgtccgga
    12421 tacggtttat cagggtatat caatgcggcg tatccggtgc ggaaatggat acggttggtg
    12481 tcggtatgga aacctgatgt tttcagacag catatacaaa aaaccgtact gcttgcgcgt
    12541 acggtttttg ccgtttcaaa tcggtttaaa gcgatttgag gcgggcgata cggttgtcca
    12601 gcgaagggtg ggtgctgagc agggagtcgc gcgtatctcc ggcgatgccc attgcgttca
    12661 tttcttcggg caaatcgacc gggttgcctt tgagcctttg cagggcggaa atcattttcg
    12721 gcgcgccgac cagttttgcc gcgcccgcat cggcgcggta ttcgcgttgt cggctgaacc
    12781 acatgacaat taagctggca aggaagccga acaggatttg gaataccatg ctgaccagga
    12841 aataagttcc ctgggactgg ctgccgtcgt tgtttcgggc aatcaggttg gcaataatgc
    12901 gcgacaggaa cacgacaaag gtattgacca cgccttgaat cagcgtcagc gtaaccatat
    12961 cgccgttgcc gacgtgtgcc atttcgtgcg ccaatacggc ttccacttcg tcacgcgtca
    13021 tatggtcgag caaaccggtg ctgacggcga tcagggagct gtttctcgat gcgcccgtgg
    13081 caaaggcatt gggttcgggg gagtggtaga tggcgacttc gggcgttttc aggttccatt
    13141 gccgcgcttg ggcttcgaca gtgttcaaaa gccaggcttc ttcttcggtg cgcggcgtgt
    13201 cgataacttc cgcgccgacc gattgtttgg cgataaattt ggacatcagc agcgaaataa
    13261 tcgaaccagt gaagccgacg acggcggaat acgccaacag gctgcccgtg ccgccccggc
    13321 tgttgatgcc caaaaccgcc aaaacaatgt tgattacgac caaaacagcg atattggtag
    13381 ccaaaaacag aaaaattcgt ttcacggatg ttcctttttg gtagggtgtg atgttttgaa
    13441 attttggggg attgtcccaa aaagttgccg gcttgtgaat atcagactcg gcaaaggtat
    13501 gcaaaacatt tgcttgcaaa tggcagtttg tgcagttggt ttttgaacta ttgtgccaag
    13561 ccgtgtagaa tcgtaaacca tctgtttgat tccaataaac acatttcaaa ggatcacttc
    13621 atgaaagcat tacttttagg cgcgccgggc gcgggcaaag gcactcaggc gcaattcatc
    13681 accgcagcgt tcggcattcc gcaaatctct accggcgaca tgctccgtgc cgcgattaag
    13741 gcaggcacgc ccttgggttt ggaagcgaaa aaaatcattg acgaaggcgg cttggtgcgc
    13801 gacgacatca ttatcggcat ggtcaaagaa cgcatcgcgc aagacgactg caaaaacggt
    13861 ttcttgtttg acggtttccc gcgcacattg gcacaagccg aagcgatggt tgaagcaggc
    13921 gtggatttgg atgcagtcgt tgaaatcgat gtgcctgaca gcgtgattgt cgaccgcatg
    13981 agcggccgcc gcgtgcattt ggcttccggc cgtacttacc acgttaccta caacccgccc
    14041 aaagttgaag gcaaagacga cgtaaccggc gaagatttga ttcagcgcga cgacgacaaa
    14101 gaagaaaccg tgaaaaaacg ccttgccgtt taccacgagc aaaccgaagt tttggtcgat
    14161 ttttacagca aactggaagg cgaacacgcg cctaaataca tcaaagttga cggcacccaa
    14221 gcagtagaag ccgtgaaagc cgaagtattg ggcgcattgg gcaaataaat cgaaaaggtc
    14281 gtacccacgg gcaggcttcg cactctgaaa acagaaaatc aggttttcag acgacctgtt
    14341 tttgataaac agcgtgttgc aaccgaaaaa taatcatttg gcgtcattcc cgcgcaggcg
    14401 ggaatccatt tctgaatttg ggcaatcgct gtttaaatct gatgaactga gttttatcaa
    14461 tggattcccg cctgcgcggg aatgacggct gatgtaccgg ttcaaattta tccgaaacag
    14521 tttgtcggag gcttgagtcc gcgtaggtcg gacatcaatg cccgacctac ggtttgaatt
    14581 tacgttgtta tagtggatta acaaaaatca ggacaaggcg acgaagccgc aggcagtaca
    14641 gatagtacgg aaccgattca ctttgtgctt cagcacctta gagaagcgtt ctctttgaac
    14701 taaggcgaga caacgccgta ccggtttaaa gtt
//
LOCUS       DAWHRS010000038        14144 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWHRS010000038 DAWHRS010000000
VERSION     DAWHRS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.038"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..380)
                     /locus_tag="WIB82_000803"
     CDS             complement(<1..380)
                     /locus_tag="WIB82_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842133.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WIB82_000804"
     CDS             complement(438..803)
                     /locus_tag="WIB82_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842134.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WIB82_000805"
     CDS             complement(785..1330)
                     /locus_tag="WIB82_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842135.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1493..1765)
                     /locus_tag="WIB82_000806"
     CDS             complement(1493..1765)
                     /locus_tag="WIB82_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842136.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIG
                     YVLIKNILVSVSDEDGTKYLAIYLSNLFFGTVLMYVLVKRLSKKPS"
     gene            complement(2327..2800)
                     /locus_tag="WIB82_000807"
     CDS             complement(2327..2800)
                     /locus_tag="WIB82_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842137.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            complement(2889..3113)
                     /locus_tag="WIB82_000808"
     CDS             complement(2889..3113)
                     /locus_tag="WIB82_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842138.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            complement(3383..3670)
                     /locus_tag="WIB82_000809"
     CDS             complement(3383..3670)
                     /locus_tag="WIB82_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842139.1"
                     /translation="MDDLIAAFLGYLSGKYREGKISRLGISLTAGSIFFVVFFIHELI
                     IFIVDGNKENLWSFITGIMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
     gene            complement(3905..4177)
                     /locus_tag="WIB82_000810"
     CDS             complement(3905..4177)
                     /locus_tag="WIB82_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842140.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(4246..4680)
                     /locus_tag="WIB82_000811"
     CDS             complement(4246..4680)
                     /locus_tag="WIB82_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842141.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNREAHWTAIGGGALIG
                     AMNPINGGRALINGMRGVAVGAVAGGINGYAGSTGKNTDIRR"
     gene            complement(4723..5592)
                     /locus_tag="WIB82_000812"
     CDS             complement(4723..5592)
                     /locus_tag="WIB82_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ3842142.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            5756..6679
                     /locus_tag="WIB82_000813"
     CDS             5756..6679
                     /locus_tag="WIB82_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ3842143.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(6757..7398)
                     /locus_tag="WIB82_000814"
     CDS             complement(6757..7398)
                     /locus_tag="WIB82_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ3842144.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(7453..8244)
                     /locus_tag="WIB82_000815"
     CDS             complement(7453..8244)
                     /locus_tag="WIB82_000815"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ3842145.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            8410..8485
                     /locus_tag="WIB82_000816"
     tRNA            8410..8485
                     /locus_tag="WIB82_000816"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8443..8445,aa:Thr,seq:tgt)
     gene            8520..8595
                     /locus_tag="WIB82_000817"
     tRNA            8520..8595
                     /locus_tag="WIB82_000817"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8553..8555,aa:Thr,seq:tgt)
     gene            8737..9528
                     /gene="panB"
                     /locus_tag="WIB82_000818"
     CDS             8737..9528
                     /gene="panB"
                     /locus_tag="WIB82_000818"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ3842146.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVLAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            9651..10487
                     /locus_tag="WIB82_000819"
     CDS             9651..10487
                     /locus_tag="WIB82_000819"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ3842147.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGIAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFHIVSPDIACFGKKDYQQLAVIKGFVED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLVQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRADTLEVARAGDKKLVVLAAACLGTTRLIDNLEIK
                     LP"
     gene            10652..12496
                     /locus_tag="WIB82_000820"
     CDS             10652..12496
                     /locus_tag="WIB82_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842148.1"
                     /translation="MLPNRFKMLTVLTATLIAGQVSAAGGGAGDMKQPKEVGKVFRKQ
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGREKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAY
                     GRGTEEQRSRAALTAAMMYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            12636..13217
                     /locus_tag="WIB82_000821"
     CDS             12636..13217
                     /locus_tag="WIB82_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ3842149.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            13227..14072
                     /gene="ispE"
                     /locus_tag="WIB82_000822"
     CDS             13227..14072
                     /gene="ispE"
                     /locus_tag="WIB82_000822"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ3842150.1"
                     /translation="MNIADGRQAFSAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHESLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
BASE COUNT         3674 a         3173 c         3568 g         3729 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtgt ggaacacttg gtcttcaaat gcctgattgt
     1261 ccaagccgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtgattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtcccaaaa aaaagatttg
     1561 ataaatatat tgcaagatat tttgttccat cttcatccga aacagaaacc aatatatttt
     1621 taattaatac atagccgatt attacattta ttattcctat aatatataag gatcttgcca
     1681 aaattttttt tgattttatt ttaggaatat ctcttatcca tgctaaaata cagcccaatg
     1741 tcgaaaagaa aataacagca aacactacct tacctcctct cacttccaac caaaccgata
     1801 gcaggtttgg ttggaagttg gtttatttat tatttaacgg cgaatgtcag tgttcttacc
     1861 cgtagaacct gcataaccat ttatgccacc agcaacagcc cccattgcta cacctcgcat
     1921 accattaatc agaattcttc ctattttttc gaatccacta tttcttccaa agatgcagca
     1981 aacgcaggtt gagcctagac ccaatctacg tttgccgccg catctgatcc ctattgtttc
     2041 ttatccttac atcttcctgc cttgtcaatc aaataaagac agaagactat acaaaaactg
     2101 accgacatac tgaaaatacc tatccccttc catgcaatct ccgcaccatt gacccaatag
     2161 atcagactga gaaacaacaa agccacagta taaatcaacg taaaaaatat tgcgtaaagc
     2221 acaataaagg gaacttttat ctcacggttg ttttttataa tatattcagc aacttgattt
     2281 ccgaatatac ctgataaaaa atatagtatt agatcatcca tttcgtttat cttctatgtt
     2341 ttcccattgc ggcgctagaa gactgattta atgctgcacc atttgcacga ataactgcat
     2401 taggaatagc atttccccct ttccaagcag aacgggcaaa aacactagta gtaatccctg
     2461 caccgcgcaa catcgcactg ctatatccgc ctcctatcat accaccagcg gttgcagcca
     2521 aggtacttct tgttgaagca aattgccctg ttttaatttt agaaatgccg tgattagccc
     2581 aagcactaat tgcccctccc atcaaagcac ctgccacaat aggaagaaac gccccctcag
     2641 tctccttcat ctccttttga gaaagctccg ccaactgcat cggcgcatct gcctgcgtgt
     2701 ggaatacgta tttttcaaat gcctgattgt ccaatccgtt tgccattgcg ggggcgatta
     2761 tagacagcat catcgtggct tcagtgattt gttttttcat aacaaattct tagggaggag
     2821 ataatgttcc atcaaaatcc tcctgaaagg ctgcctgaaa tttccaagca gcctttttga
     2881 ttgcttaatt aatcggatag tgcgacaaat ataaagtggg cggcaatcat tgccgtaacg
     2941 gcagccataa ctgttttatt ttcttcaaac caaagcaagt tgagaaacat aaacatcaag
     3001 aaatatgatg gttcttcagt tttgccgctg ctttcttgag ttagaagata aaatatagat
     3061 atggcggtta tgccgattgc tgcttttatt aaaatatatt tgtttttcat catcttgtat
     3121 ggtttttcat gcaagtttca acaataaaat tcggaataag atagagagat gttgatattt
     3181 cagataaata tacaaaacaa tcaagaaact attggcggta tgtataatct tgaatttctt
     3241 tttttgatga aattaaaata atgaaatata tggtaaacgt tacaacggaa gccttagcct
     3301 cccgattgca gtatttcaat tccaagataa atgccgcata gaagataaaa tactcaaatg
     3361 gttttttagc ggcttaaata tcttatttaa tcttattgat tgttttccca atggtttccg
     3421 caataaataa taagaagtag aataataaac aaacagaaca tgaaaataaa aataaatctt
     3481 tcattatccc agtaataaaa ctccaaagat tttccttatt tccatcaaca ataaaaatta
     3541 tcagttcatg aataaagaat actacaaaga aaatgcttcc tgccgttaga gatattccta
     3601 acctgcttat ttttccttct ctatatttac ctgataaata gcctaaaaaa gctgcaatca
     3661 aatcatccat tttaaaaatt tctgtaaaaa caggtaatgt ctttaattca ttacctgttt
     3721 tgccactata tctatgatat aagcgtctct tacccttcag cctctttggg aaatcaaagc
     3781 cctgcttaga acgtatgttg cacacccaat ttcaataggt aaatcagatt gcaaatccag
     3841 caatttgaat attgtcattg ttccgtgcaa aagggatttt tattgatgag ttgtgtactg
     3901 ggtttcagct tggcttttta gataatcttt tcacaagaac atacatcaat actgtccaaa
     3961 aaaaaagatt tgataaatat attgcaacat attttattcc acctccatcc gaaacagaaa
     4021 ccaatatatt tttaattaat acatagctga ttattacatt tattattcct ataatatata
     4081 aggatcttgc caaaattttt tttgatttta ttttaggaat atctcttatc catgctaaaa
     4141 tacagcccaa tgtcgaaaag aaaataacag caaacactac cttacctcct ctcacttcca
     4201 accaaaccga tagcaggttt ggttggaagt tggtttattt attatttaac ggcgaatgtc
     4261 agtgttctta cccgtagaac ctgcataacc atttatgcca ccagcaacag cccccactgc
     4321 tacacctcgc ataccattaa tcagtgctct tcctccgtta atagggttca tcgcaccaat
     4381 taacgcacca cctccaattg ctgtccaatg tgcctctcga ttatacgagc cactagcaat
     4441 agcactagcc atatagctga aatgccctcc aactcctccc ataatgcctc caacagcatt
     4501 aggaatccac tccccctcag tctccttcat ctccttttga gaaagctcag ccaactgcat
     4561 cggcgcatct gcctgcgtgt ggaatacgta tttttcaaat gcctgattgt ccaatccgtt
     4621 tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt gttttttcat
     4681 aataactcct ttggattaca aggttggaaa atcaaaacct gcttaaaatg tatgctgtac
     4741 gccaaatttc agttcggaac tgctttgccc tgaaacgttg aaacgtgcgg atgcgtttaa
     4801 agccgtggtt ttggtgaaac cgaaacctgc gccgaaatgg gcgtaggtgg atgtgtttct
     4861 tgcggattct tttttgccgt ctatgcggtc gggctgtttg cccagccatt ggatgcctcc
     4921 ggtcaggctg attctgtcgt tggcggcaaa tgagatgttg gggttgagca gcaggtagtt
     4981 gcccgatttg tagcggatgc cgtctgaaag ggttttgctg ccgttgatgc ggtaggcggc
     5041 ggtgagggaa aggacaatcg gatctatggc tttgtaggtg gtggcgccga tgagccacga
     5101 ttttcccgac gaggctttgt tgcgcgattt ttcgtaaacc gtgctttcaa gaaagctgat
     5161 tagggcgggg tttttgtcgt ctttaaggaa agtgtggctg atgccgaggg atacgtcgga
     5221 catccgtttg ttgcgggttt tgccgttgcc gtcgagtttg cgttcttcgt gccacagata
     5281 gctgccgctg ccgtaaatgt cggtattccc ggtcagtccg tagcgcaaac cgagcgtgcc
     5341 gacgagcata tcggtattgc tgccgttttc ttggatttcg gtcggaatgg ggataaacga
     5401 ggttgcgccg gtttgaatgt aaaccggtgc ggcaagttcg gcgcggttgt tttcgctgtt
     5461 caggtaggta agggaagttt ccagtttcca ttttcccttg tcggtcatta tgtcttcaat
     5521 cgtcaagggc aggtcggcat aagtggataa aggcaggatg gcgggcaagg cgggcaaaaa
     5581 gatgcgcttc atatttctcc tattgatatt aattcttatt tactgataat aatgaatatc
     5641 cgataatctt gtctaaataa atttttaaat cataaaaaac aatatgtttg tttttgtcaa
     5701 ttatgtttcg atatgccgtc tgaaaagttt gtgaaaaacg gttacaatcc gccgcatgaa
     5761 aaaacgcaat aatcctcttc cgctgttgaa cggtgtcaaa cccagttatt tggtgctgcc
     5821 gcatgaaaag cagttttacg ggctgccgct gctgcatttt ctgtgcatcc gctttccttt
     5881 tgtgggcgcg gacgattggc gcaggcggtt gaacagcggt tttgtggtcg gttcggatgg
     5941 tgcggcgttg gacgaacatt ctttgttcga gccgggtaag gtgatgtttt attaccgtga
     6001 aaccagccgt gagagcgagc cgcgtattcc gtttgaagaa aagattttgc atattgatga
     6061 gcatttgatt gtggtggaca aaccgcattt tctgcccgtc atccccagcg gcaggttttt
     6121 gcgggaaacc ctgctcacgc gcctgcgttt gcggcctgaa ttgcagcatt tgaatgttga
     6181 ggacattacg ccgctgcacc gcttggacaa ggatacggca ggcgtgatgc tgctgtcgca
     6241 taatcctgcc acgcgcggag cctatcagac gatgtttcaa aacaaaacgg tatggaaaac
     6301 gtatgaggcg cttgcgccga caaggacgga tttgccgtat ccgctcgatg tggtttcgcg
     6361 tttggtgagg ggtgagaaat tttttacgac gcaagaggcg gaaggcgaac caaacgcaca
     6421 tacgacggtc gaactgattg aaaacagggg ggaattcagc ctttaccgcc ttacgccgca
     6481 tacgggcaag aaacaccagt tgcgcgtgca tatgatgggt ttgggtatgc cgctgttgaa
     6541 tgacgcgctc tatcccgtgc cgtctgaagc gggcagcgag gattatcgga aacctttgaa
     6601 gctattggca aaaaagattg cgtttgcaga tcctttgtcg ggtcgggaaa gggtgttttg
     6661 cagcggattt tgcctataat gagagggaac gcaaacccgt atcggcggat gccgccgata
     6721 cgggttttac attatcgatg ccgtctgaaa ggggttttat ccgttcaggc atacgggcgc
     6781 gaagcggagg atggtgtcgc gcaggacgat gagtttgccg tgaaagaaat gcgtggaacc
     6841 ggcgatggta atgacgggca aatcttgcgg ttccgcccat ttcagtgctt tcccgatttc
     6901 gacgacttcg tcttccgcgc cgtgtatcat cagcgttttg gcaacattgg gaacggcgga
     6961 cggctcgggg cggccggtat agtggcagac tgccgcgccg atgagcagca ataaatcggg
     7021 tatgcgcgct tgtgcggcaa atgtggcgac ataaccgccg aaggagaagc cggataaggc
     7081 aaattcgggg gcttcgggat gttgggcacg ggcatagtcg atgacggcga ggcagtcttg
     7141 cgtttcgccg cgtccgtaat cgtgtgtgcc ttcgctgccg cctacgccgc gaaggttggg
     7201 caggtagcag tggaagccga gtttgcttaa ggctttggcg gcagtttgga taactttgtt
     7261 ggtgtttgtt ccgccctgga gggggttggg atgattgatg acggcaacac cgcgtgccgg
     7321 tacttgttcg gacgggatat ggatggtttc taaaatgccg gcaggaccgg atatgtgtat
     7381 ggtttcgggt ttcagcataa tgatttcctc ttgattttcc gcatgttccg atgcagccgt
     7441 gaagatagtt gcttaaaggt ggaaatgctt gccgttgttg gggttggacg ctttcaggcc
     7501 ggcaagcata cggtggaagt ccaatccgta tgcgttgctc agtttgtcgg cacgtttttt
     7561 gagtttgtct atgttttgct ctttggggct gctttggaag gccattaccg cgacattgcc
     7621 gtggctttcg gcaggaagtt ccaagacgcg cccttcaaaa acgctcaaca accgttcgat
     7681 gaagcgttga tagcgtttgt cgccgctcca ccagttggta acgaatatgc cgtctgaaga
     7741 gagtgcgttg cggcagtctc ggaagaacgg ttcttcaacc agcgcatcga tgatttgttc
     7801 gccgtcgaac ccgtccacca agatgatatc ggtgttgtgg cggaagactt tgatatattc
     7861 cgcaccgtct gcttcaataa tttcaaattt ctcgccctcg aaaggcaact caaacaggtt
     7921 gcgggcaatg gcgatgacct gaggattgat gtccacggcg gtttggcgcg tgtcgggcag
     7981 gtaggtatct atccagcgtg caaacgagcc gccgcccaag ccgatttggg tgatatgttg
     8041 cggaagagcg tcggtaaaca gcagccagcc catcatcgcg cggctgtaag agagcaccag
     8101 ctcggacggg tggtcgaggt tcatcgagct ttgaacggtg tcgctgccca agtgaagcga
     8161 acggatattg ccttcttcgg aaatgccgac ttcgggaaag ccggattttg caggacgcag
     8221 gcggcggtag ggatgtcttg ccatcggtgt gaacggtcgg tttgaaaggc ggatatttta
     8281 tcagaatgcc gatgcttgtt ctgtttcaaa ttaatttctt ttcaaataaa ttacttattc
     8341 ggatttgccg gggctttcgg ataaattcct tgccaaggtg cggcattgcc tgcataattc
     8401 gctttctttg ccggtatagc tcagttggta gagcacctga cttgtaatca gggggtcccg
     8461 agttcgactc ttggtgccgg caccatttct gctgatggtg ttgcaaaaca tttcagtaag
     8521 ccggtatagc tcagttggta gagcacctga cttgtaatca gggggtcccg agttcgactc
     8581 ttggtgccgg caccagattt gacagtccga ttgtgtaaaa cagtcgggct gttttgcatt
     8641 tgcggcacgg tcggtggcag ggctgcgggc atttcactat aatagcccgt ttcaaactga
     8701 aaaggccgtc tgaaaagggc ggggtaacaa tatctgatga ttactgtgaa cacactgcaa
     8761 aaaatgaagg cggcgggcga aaaaatcgct atgctgaccg cttacgaatc cagttttgcc
     8821 gcgctgatgg acgatgccgg cgtggaaatg ctgctggtcg gcgattcttt ggggatggcg
     8881 gttcaggggc ggaaatcgac gctgcccgtc agcctgcgcg atatgtgtta tcacaccgaa
     8941 tgtgtagcac gcggtgcaaa aaatgcgatg attgtcagcg atttgccgtt tggtgcatat
     9001 cagcagagta aggagcaggc gtttgccgcc gccgccgaac tgatggctgc cggcgcgcat
     9061 atggttaaac tcgaaggcgg cgtgtggatg gcggaaacga ctgaattttt gcaaatgcgc
     9121 ggtattccgg tttgtgcgca catcggtctg accccgcaat ccgtgtttgc cttcggcgga
     9181 tataaagttc aggggcgcgg cggcaaggcg caggcgttgc ttaacgatgc caaggcgcat
     9241 gacgatgcgg gggcggcggt cgtgctgatg gagtgcgtac tggcggaact ggcaaaaaag
     9301 gtaactgaaa ctgtttcctg tccgaccatc ggcatcggcg cgggtgcgga ttgcgacggg
     9361 caggttttgg tgatgcacga tatgctcggc attttcccgg gtaagacggc gaaattcgtc
     9421 aaaaacttta tgcaggggca tgatagtgtt caagcggcgg ttcgggcgta tgttgccgaa
     9481 gtcaaagcca aaaccttccc tgctgcggaa catatttttg cagattgagc ggtttacatg
     9541 cagcatgccg tctgaaagcc gtttcagacg gcattttgtt ttgccgtgcg cggcgcgtat
     9601 aatcggcgcg ttttgtcggg caggaagccc gaaggataag gattaccgtt atgcaaatca
     9661 tacataccat tcgagaactg cgcgcgtggc gtaaaaatgc gggaaaggtg gcatttgtgc
     9721 cgaccatggg caatctgcat gaaggacatc ttgcgcttgt gcgtgaggcg aaaaaacgcg
     9781 cggacagtgt cgtggtcagc attttcgtca atcgcctgca attcggtcag ggcgaggatt
     9841 tcgacaaata tccgcgcact ttgcaacagg atgcggacaa acttgccgcc gaaggcattg
     9901 ccgttgtttt cgcgcccgat gagaaagaac tctatccgaa cgtggaacag cgttacaacg
     9961 tcgaaccgcc caatctgcaa aatgagttgt gcggcaaatt ccgcccgggg cattttcgcg
    10021 gtgtggcaac ggttgtttct aaattgttcc acatcgtttc cccggacatt gcctgttttg
    10081 gtaagaagga ttaccagcag cttgccgtga ttaaaggttt tgtcgaagat ttgaattttg
    10141 atgttgaaat agtgcctgtt gatacagggc gcgcggaaga cgggttggca ctgtcgagcc
    10201 gcaaccagta tttgagtgcg gcggaacgcg acgaagcacc gcgcctgtac cgcgaattaa
    10261 aggctgttgc cgaatccttg gtgcagggca gtttggatta tgcaggtttg gaaaaacgtg
    10321 ccgtccaatc cctgacagaa tacggctggg tggtcgatta tgtcgaaatc cgccgcgccg
    10381 atacgctcga agtggcgcgg gcgggagata agaaactggt ggtcttggcc gccgcctgtc
    10441 tggggacgac gcgcctgatt gacaatttgg aaataaaact cccttaaacc gcaagcgtcg
    10501 ggaatgccgt ctgaagcgga tttgcgtttc agacggcatt tatttttttg aacggggttt
    10561 cgcaaatcta caaacgattc tgcttgtgat aaagttacgc ctgattatat gccgtctgaa
    10621 ggttcggacg gctgtcggat aaaggatgat tatgttacct aaccgtttca aaatgttaac
    10681 tgtgttgacg gcaaccttga ttgccggaca ggtatctgcc gccggaggcg gtgcggggga
    10741 tatgaaacag ccgaaggaag tcggaaaggt tttcagaaag cagcagcgtt acagcgagga
    10801 agaaatcaaa aacgaacgcg cacggcttgc ggcagtgggc gagcgggtta atcagatatt
    10861 tacgttgctg ggaggggaaa ccgccttgca aaaggggcag gcgggaacgg ctctggcaac
    10921 ctatatgctg atgttggaac gcacaaaatc ccccgaagtc gccgaacgcg ccttggaaat
    10981 ggccgtgtcg ctgaacgcgt ttgaacaggc ggaaatgatt tatcagaaat ggcggcagat
    11041 tgagcctata ccgggtaagg cgcaaaaacg ggcggggtgg ctgcggaacg tgctgaggga
    11101 aagaggaaat cagcatctgg acggactgga agaagtgctg gctcaggcgg acgaaggaca
    11161 gaaccgcagg gtgtttttat tgttggcaca agccgccgtg caacaggacg ggttggcgca
    11221 aaaagcatcg aaagcggttc gccgcgcggc gttgaaatat gaacatctgc ccgaagcggc
    11281 ggttgccgat gtggtgttca gcgtacaggg acgggaaaag gaaaaggcaa tcggagcttt
    11341 gcagcgtttg gcgaagctcg atacggaaat attgcccccc actttaatga cgttgcgtct
    11401 gactgcacgc aaatatcccg aaatactcga cggctttttc gagcagacag acacccaaaa
    11461 cctttcggcc gtctggcagg aaatggaaat tatgaatctg gtttccctgc acaggctgga
    11521 tgatgcctat gcgcgtttga acgtgctgtt ggaacgcaat ccgaatgcag acctgtatat
    11581 tcaggcagcg atattggcgg caaaccgaaa agaaggtgct tccgttatcg acggctacgc
    11641 cgaaaaggca tacggcaggg ggacggagga acagcggagc agggcggcgc taacggcggc
    11701 gatgatgtat gccgaccgca gggattacgc caaagtcagg cagtggctga aaaaagtatc
    11761 cgcgccggaa tacctgttcg acaaaggtgt gctggcggct gcggcggctg tcgagttgga
    11821 cggcggcagg gcggctttgc ggcagatcgg cagggtgcgg aaacttcccg aacagcaggg
    11881 gcggtatttt acggcagaca atttgtccaa aatacagatg ctcgccctgt cgaagctgcc
    11941 cgataaacgg gaggctttga gggggttgga caagattatc gaaaaaccgc ctgccggcag
    12001 taatacagag ttacaggcag aggcattggt acagcggtca gttgtttacg atcggcttgg
    12061 caagcggaaa aaaatgattt cagatcttga aagggcgttc aggcttgcac ccgataacgc
    12121 tcagattatg aataatctgg gctacagcct gctgaccgat tccaaacgtt tggacgaagg
    12181 tttcgccctg cttcagacgg cataccaaat caacccggac gataccgctg tcaacgacag
    12241 cataggctgg gcgtattacc tgaaaggcga cgcggaaagc gcgctgccgt atctgcggta
    12301 ttcgtttgaa aacgaccccg agcccgaagt tgccgcccat ttgggcgaag tgttgtgggc
    12361 attgggcgaa cgcgatcagg cggttgacgt atggacgcag gcggcacacc ttacgggaga
    12421 caagaaaata tggcgggaaa cgctcaaacg tcacggcatc gcattgcccc aaccttcccg
    12481 aaaacctcgg aaataatgca ggtccatcct ttcagacggc ataaggtttg ccgggaagcc
    12541 ggggcattcg ggcaaacggc acgcagttcg cacgcgtttt gcacggcacg ccgaacccat
    12601 cggccggcag gatggcatcc gttaaggaaa ttctgatgaa acacaccgta tccgcatcgg
    12661 tcatcctgct tttgaccgct tgcgcgcaat tacctcaaaa taacgaaaac ctgtggcagc
    12721 cgtccgaaca catcagcagt tttgcagcag aagggcggtt ggcagtgaaa gcggaaggga
    12781 aaggttcgta tgcaaatttc gattggacat accaaccgcc cgtggaaacc atcaatatca
    12841 ataccccttt gggcagtacg ctcgggcagt tgtgtcaaga cagggacggc gcattggcag
    12901 tggacggcaa aggaaatgtc tatcaggcgg aaagtgcgga agaattgagc aggcagctgg
    12961 tcggtttcaa actgccaatc caatatctgc atatctgggc agatggcagg cgtgtggcgg
    13021 gcgcgcctta ccgcatcctg ccggacggca tattggaaca atacggttgg actgtcggca
    13081 gaaccgccga cagtgggggg caagtccgaa cgttgcaact gaataacgga aatttgaaca
    13141 tcaggctggt tttcaccgaa atcggtatgc cgtctgaaac cgaaaccccg gaacgctgtg
    13201 cggcgcgcac gagataaggc ggacagatga atattgcgga cggacggcag gcgttttccg
    13261 cacctgcaaa actgaatctc gatttgagga ttaccggcag gcgggaagac ggttatcaca
    13321 atatcgaaag catattctgc ctgatagatt tgcaggatac cgtatatttg aaaccgaggg
    13381 acgacggcaa aatcatcctg cacaatccgg ttgatggaat gccgcaggaa gtagatttga
    13441 gctaccgtgc cgcatcgttg ctgcaaaaat atgcgcgcaa ccccgccggc gtggaaatat
    13501 ggctggacaa aaaaatcccg acaggggcgg gtttgggcgg cggaagctcg gatgcggcaa
    13561 ccgttttgct ggtgttgaac cgttggtggc agtgcggtct gacgcagcgg cagctcattg
    13621 attcgggcgc ggctctgggg gcggacgtac cgttttttat tttcggcaaa aatgcgtttg
    13681 cgcggggtat aggcgacagg ctggacgaaa tggatattcc gaaacagtgg tatgtcatcg
    13741 tcaaaccgcc cgtccacgtt tccactgcaa aaattttcac acacgaaagc ttgacacgaa
    13801 attccgcctc aagcataatg ccgactttcc aaaatctgca accgtttaga aatgatatgc
    13861 aggcagttgt atttaaagaa taccctgaag tttggaaagc ctattccgag ttgtcccgat
    13921 atggatttgc cttaatgaca ggttccggtg cgtgtgtatt cacggcgtgt caagatagga
    13981 atagcgcata caatatatac cgacaagttt cagatttgta cgaggcatat ttggcagagg
    14041 gtctttcaaa acatcctttg ttgtccgtat aaacattgtt ggggagtcgt caagcggtta
    14101 agacactgga ttttgattcc agcatgcgaa ggttcgaatc cttc
//
LOCUS       DAWHRS010000039        13928 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWHRS010000039 DAWHRS010000000
VERSION     DAWHRS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.039"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            32..463
                     /locus_tag="WIB82_000823"
     CDS             32..463
                     /locus_tag="WIB82_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ3842151.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            474..1340
                     /locus_tag="WIB82_000824"
     CDS             474..1340
                     /locus_tag="WIB82_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ3842152.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAV
                     IAGEVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSRKFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLKNSLERDNHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCVG
                     TAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IGGLERNIKAGT"
     gene            complement(1391..2443)
                     /gene="sohB"
                     /locus_tag="WIB82_000825"
     CDS             complement(1391..2443)
                     /gene="sohB"
                     /locus_tag="WIB82_000825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ3842153.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2547..2636)
                     /locus_tag="WIB82_000826"
     tRNA            complement(2547..2636)
                     /locus_tag="WIB82_000826"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2600..2602),aa:Leu,seq:taa)
     gene            complement(2739..2812)
                     /locus_tag="WIB82_000827"
     tRNA            complement(2739..2812)
                     /locus_tag="WIB82_000827"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2778..2780),aa:Cys,seq:gca)
     gene            complement(2838..2913)
                     /locus_tag="WIB82_000828"
     tRNA            complement(2838..2913)
                     /locus_tag="WIB82_000828"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2878..2880),aa:Gly,seq:gcc)
     gene            complement(2935..3010)
                     /locus_tag="WIB82_000829"
     tRNA            complement(2935..3010)
                     /locus_tag="WIB82_000829"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2975..2977),aa:Gly,seq:gcc)
     gene            complement(3034..3109)
                     /locus_tag="WIB82_000830"
     tRNA            complement(3034..3109)
                     /locus_tag="WIB82_000830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3074..3076),aa:Gly,seq:gcc)
     gene            complement(3135..3210)
                     /locus_tag="WIB82_000831"
     tRNA            complement(3135..3210)
                     /locus_tag="WIB82_000831"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3175..3177),aa:Gly,seq:gcc)
     gene            complement(3290..3853)
                     /gene="pgsA"
                     /locus_tag="WIB82_000832"
     CDS             complement(3290..3853)
                     /gene="pgsA"
                     /locus_tag="WIB82_000832"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ3842154.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            complement(3959..4382)
                     /locus_tag="WIB82_000833"
                     /pseudo
     CDS             complement(3959..4382)
                     /locus_tag="WIB82_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4713..5048)
                     /locus_tag="WIB82_000834"
     CDS             complement(4713..5048)
                     /locus_tag="WIB82_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ3842155.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            5177..6082
                     /gene="mtrA"
                     /locus_tag="WIB82_000835"
     CDS             5177..6082
                     /gene="mtrA"
                     /locus_tag="WIB82_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ3842156.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(6144..6632)
                     /locus_tag="WIB82_000836"
     CDS             complement(6144..6632)
                     /locus_tag="WIB82_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ3842157.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            6779..7681
                     /locus_tag="WIB82_000837"
     CDS             6779..7681
                     /locus_tag="WIB82_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3842158.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(7722..8852)
                     /locus_tag="WIB82_000838"
     CDS             complement(7722..8852)
                     /locus_tag="WIB82_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3842159.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            9056..12025
                     /gene="alaS"
                     /locus_tag="WIB82_000839"
     CDS             9056..12025
                     /gene="alaS"
                     /locus_tag="WIB82_000839"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ3842160.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQKQAFFYKLV
                     PDLVKAMGDAYPELKEKQTQIMEALRAEESRFGETLEKGMGLFNQVYNELKFNQIFDL
                     LAKEFKVTPVEDIDLDMFSTRNFVGRSAEIPYISNQHPNVDYPAPMGNVNEKIYAMPV
                     RLTDGRIGINIFPTDETARLILSNDWNSLRFYSFCNSLITYYQQKGQLETLKLNGEHI
                     FKLYDTYGFPYDLTADMARELGIELDEAGFEREMEAQRARARAAQSFKANAQLPYDGQ
                     DTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGYI
                     FSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATHL
                     MHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAVN
                     AAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIISE
                     GGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKAL
                     EKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTNLTGKSDNAV
                     ILLAAVNDGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDADK
                     LPAVLDSVKDWVGAKLV"
     gene            12286..12717
                     /locus_tag="WIB82_000840"
     CDS             12286..12717
                     /locus_tag="WIB82_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842161.1"
                     /translation="MSISLIGLHITIAIILFFTTNFMGKKSSIFGYYQLSFSEENHSP
                     AFNIFYRAFTPILFIVIFSWVVTSLEIPISLEKINYVVIYYFIIRLLSVFVFEKTHIV
                     NWFNQLTIPILSITLSFIVYNKMILPKSFLLPSSQEVATTF"
     gene            13017..13304
                     /locus_tag="WIB82_000841"
     CDS             13017..13304
                     /locus_tag="WIB82_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842162.1"
                     /translation="MQVTSAEYLSNEESIKKGGNILMEKYNEKYNESIDGNKTLYKSY
                     YESRRESIKNYNPDAKYINEIESIYMMLGEIYPNAPDFMSPHFEGDCSEGE"
     gene            13503..13589
                     /locus_tag="WIB82_000842"
                     /pseudo
     CDS             13503..13589
                     /locus_tag="WIB82_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            13792..>13928
                     /locus_tag="WIB82_000843"
     CDS             13792..>13928
                     /locus_tag="WIB82_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ3842163.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YL"
BASE COUNT         3606 a         3542 c         3282 g         3498 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaggcgaaca ggaccggcgc aaacggttgc tgtcgttgac
      301 cgaaacaggc aaagcctatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
      361 agtatttgcc acattcggcg acaagcgcac aactcggctg tttgccgatt tggatgcact
      421 ggctgaagtg atggaaaaaa caatctcgga aaataaaaaa taggggggca aatatgtgga
      481 aaatgttgaa acacatagcc caaacccacc gcaagcgatt gattggcaca ttttccctgg
      541 tcggactgga aaaccttttg atgctggtgt atccggtgtt tggcggccgg gcgatcaatg
      601 ccgtgattgc gggggaggtg tggcaggcgt tgctgtacgc tttggttgtg cttttaatgt
      661 ggctggtcgg tgcggcgcgg cggattgccg atacgcgcac gtttacgcgg atttataccg
      721 aaatcgccgt gccggttgtg ttggaacagc ggcagcgaca agtcccgcat tcggcggtaa
      781 ctgcacgggt tgccctgtca cgtaagtttg tcagcttttt tgaagaacac ctgccgattg
      841 ccgcgacatc cgtcgtatcc atattcggtg cgtgcatcat gctgctggtg ctggaatttt
      901 gggtcggcgt gtcggcggtg ggcatacttg cgttgttttt atggcttttg ccacgttttg
      961 ccgccatcag cgaaaacctg tatttccgcc tgaagaacag cttggaacgc gacaaccact
     1021 ttatccgaaa aggcgaccgg cggcagctgt accgccatta cggactgctt gcgcgcctgc
     1081 gtgtgctgat ttccaaccgc gaagccttcg gctatctctg cgtcggcacg gcgatgggta
     1141 ttttgttcgg ctttgctttt gtgatgatga cgctcaaagg ctacagcagc gcggggcatg
     1201 tctattcggt cggcacttat ctgtggatgt ttgccatgag tttggacgac gtgccgcgat
     1261 tggtcgaaca atattccaat ttgaaagaca tcggacaacg gataggggga ttggaacgga
     1321 acatcaaagc cggaacttga aaaatgccgt ctgaacacgc ttcagacggc atttccatcc
     1381 gttcggcaaa ctacatcaca tccgcccgcc ggttgacaag tttggcaaac aacttttcaa
     1441 cagaagcttc cgcctgcaaa ccaatgcgct ggatcaggct ttgcttctcc tgatatttca
     1501 cttcgataac ctgtttgttt tcaaacgctt tcaacaacaa atcatcactg gtcgaaatct
     1561 cgtcaatcaa gttcaacgcc aacgcctgcc gaccgaacca atgctcgccc gttgccactt
     1621 cctcaatatc caattgaggg cggttctcgc tgacaaactg cttgaacaac tgatgcgttt
     1681 cctccagttc ctgtcggaat ttctgtttgc ccttttccgt attttcaccc ataaaagtaa
     1741 ccgtgcgctt aaattcgccc gccgtcatca catccacatc aatatcatgt tttttcaaca
     1801 ggcggtggat attcggtact tccgccacca cacccaccga accgacaatc gcaaacggag
     1861 cggaaacaat tttatccgcc acacacgcca tcatataacc accgctcgct gccaccttat
     1921 cgacggcgac ggtcagcgga atattgcgtt cgcgcaaacg cctaagctgt gaagccgcca
     1981 aaccgtaacc gtgaaccacg ccgcccggac tttccaatct aagcagaacc tcatcttcag
     2041 gcttggcaat caaaagcacc gccgtaatct catgacgcaa ggattctacg gcgtgtgcat
     2101 acaaatcgcc gtcaaaatcc aacacaaaaa ggcgggattt ttgcgtttcg gcagatttct
     2161 ccccaccctc cttcaaacgc tttttctctg ctttggcttc cgccttttcc tttttctttt
     2221 cctctttttc ctgatgtttt gcctcttccc cgcttaaaaa gaatgcttca aacgattgcc
     2281 gctgtttttt ataattttcc gaaaaatccg tcagtacgac actgccgctt tccgactgtt
     2341 tcttactctg tacgatagcc aacacaatca gcgcaattgc gccgaacacg gtaagcagtt
     2401 cgagcaggaa aataccgtaa ttcagtaaaa tttctttcca cattgattgg atttcctctt
     2461 gttcaggcat gaacatgtca atattgtcca tcaccgtccg acagataaaa aaataaccgc
     2521 ttggagcggc attgtcattt tcagcttggt gcccggagcc ggaatcgaac cggcacggga
     2581 tgtttagtcc cgacggattt taagtccgtt gtgtctacct atttcaccac ccgggcattt
     2641 gtgaaaggtg gaggcggggg cgcggatttt aaccggcctg tatgaagatt gcactcctca
     2701 tagcataaac actctgccac cccgccatag tacgataatg gaggcgagag tcggaatcga
     2761 accggcgtag acggatttgc aatccgctgc ataaccactt tgctatctcg ccctaaaact
     2821 ggcttatcta aaaaacttgg agcgggaaac gagtctcgaa ctcgcgacct caaccttggc
     2881 aaggttgcgc tctaccaact gagctattcc cgcgcgttca aacatatcgg tttttggagc
     2941 gggaaacgag tctcgaactc gcgacctcaa ccttggcaag gttgcgctct accaactgag
     3001 ctattcccgc gttgatatgt ttgaaataaa acttggagcg ggaaacgagt ctcgaactcg
     3061 cgacctcaac cttggcaagg ttgcgctcta ccaactgagc tattcccgca atgattgtgg
     3121 gaaaatgaaa tttttggagc gggaaacgag tctcgaactc gcgacctcaa ccttggcaag
     3181 gttgcgctct accaactgag ctattcccgc ccgatttcat tctccaacat cgaagagacg
     3241 caattattat ggattctgtt tttgccgtca agctattttt atgttttttt caggcgattt
     3301 ctttccacgc cattttcaga taatacagca tcgaccaaac cgtcagcaaa gatgcgataa
     3361 acatcaatat gttgccgata acaacaagat taaatccgta aaaatcggga atattcagca
     3421 gcagcaggaa aattgccagc atttgcgcgg cggttttaaa cttaccgacg gtggcgacgg
     3481 caacgctgtt ccttttgccc atttgcgcca tccattcgcg caatgcggaa atggtaattt
     3541 ccctgccgat aatgatcatg gcaaacaaaa cataggtccg gtcgagtttg accagtaaaa
     3601 gtagcgatac ggcaaccatc agcttgtcgg cgacgggatc gaggaaggcg ccgaaatcgg
     3661 aagtctgttt ccacagtctt gccaaaaatc cgtcaaacca gtcggttaag gcggcaacgg
     3721 caaaaatgac ggcggcggtg agattaatcg tttcctccgc gaaccacgga aaaggcaggt
     3781 aaaaaagggc tgtcatgaca ggaatgagca agaccctcaa ccatgtgagg aagataggga
     3841 gattccaagg catcggtttt ctctgtgcag actgtaaagt tgtgattata acggttatcc
     3901 tcataaccca aaacgtaaaa ttgctgcatg ggcattcccc cgccccgcca atctgttttc
     3961 acattctttt caaacgcagg aaaatggcgg gcaataaaag caaaataccc agtttcaggc
     4021 agaaaacggc aggttgtgcc aacacttcga caaggcggtc ttccgtgcgg gcaaaatctt
     4081 tattgcttat agacactgcc actgttgcgg tattccaaca gaacgccgtt taaaaaacct
     4141 ttgccgacgg tttcgcttaa aacggctcta acctgctccg ccctgatggt tctgccgata
     4201 ttgccgcctg tgcacaaact gtcgaaccca tagcaggaaa gccggtaatg ctgcccgtct
     4261 gcatccagtt tgattgcccg tccgctgcgg ttgggggcgg taacggtcaa ttccgcatat
     4321 tcgaatgttt tttcttgttc gtgaaatgcc gtcaggtaag gtgcaataaa aacggcggac
     4381 aacagcagac agcttatggc ggcaaaccat acccagcgat aatatagtgg attaacaaaa
     4441 accagtacag cgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca
     4501 ccaagtgaat cggttccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     4561 tttgttaatc cactatattt cacgcttacc ccttgtttct caaatgccgt ctgaaaaaac
     4621 ggctgcatat gttgtctaca aaatatcggg aagcataaca ggcaaaatgc cgtctgaaat
     4681 attttcagac ggcatttctt atccgaaacg gattattttt gcgtttcaac cgcttccaat
     4741 gcacgcaggg cataagtgta agcggcaccg gcattcaggg cgatggcggt tgccaatgcg
     4801 cctgcgattt cgctgtcggt cgcaccggct ttggcggcag cggcggcgtg aacgctgatg
     4861 cagctctcgc aacgagtggt aatggcaacg gcgatggcaa tcagttcgcg cgttttggca
     4921 tcaagtgctt cggcggcggc ggcctgttcc aatgcgccgt aggcctgcag cattttagga
     4981 tgcgccttac ccagctcgcc gaacgatttt ttaaccaatg cggtatgttc tttccaatct
     5041 ttaaacattt tcttttcctt tctcttgcgt ttaaccctga tacgcgcttg cgtatctgtt
     5101 ttcgatgtgc gtattattgc aattattcag ttgtgtttct cgtttaatca tctcatttta
     5161 tggttcaaaa agatttatgg acattctgga caaactggtc gatttcgccc aattgacggg
     5221 cagtgtggat gtgcagtgcc ttttgggcgg acaatggtcg gtacggcatg aaaccttgca
     5281 acgcgaagga ttggtacaca ttgttacatc gggcagcggc tatctctgca tcgacggcga
     5341 aacttccccg cgtccggtca gtacagggga tattgtattt ttcccgcgcg gcttgggtca
     5401 tgtgttgagc cacgacggaa aatgcggaga aagtttacaa ccggatatgc ggcagcacgg
     5461 tgcgtttacg gtcaagcagt gcggcaacgg acaagatatg agcctgtttt gcgcccgttt
     5521 ccgctacgac acccacgccg atttgatgaa cgggctgcct gaaaccgttt ttctgaacat
     5581 tgcccatccg agtttacagt atgtggtttc aatgctgcaa ctggaaagca aaaaaccttt
     5641 gacggggacg gtttccgtgg tcaacgcatt gtcgtccgtc ctgctggtgc ttatcctgcg
     5701 cgcctatctc gaacaggata aggatgtcga actctcgggc gtattgaaag gttggcagga
     5761 caaacgtttg ggacatttaa tccaaaaggt gatagacaaa ccggaagacg aatggaatgt
     5821 cgacaaaatg gtggcggctg ccaatatgtc gcgcgcgcaa ctgatgcgcc gtttcaaaag
     5881 ccgggtcgga ctcagcccgc acgcctttgt gaaccatatc cgcctgcaaa aaggcgcgtt
     5941 gctgctgaaa aaaaacccgg attcggtttt gtcggtcgca ctgtcggtag gctttcagtc
     6001 ggaaacgcac ttcggcaagg cgttcaaacg gcaatatcac gtttcgccgg gtcaataccg
     6061 gaaagaaggc gggcaaaaat aaatcggggc ttcaaacgca aatgccgtct gaaaaggctt
     6121 tcatacagca tttgcgtacc gcgtcatttc aagggctgca tcttcatcac ttccatcaaa
     6181 aagttggtaa atgcggggtt gttgggtttg acatccatat ttttccaacg ctgctgccag
     6241 ccgcgcaagg cattctggat atacagcttg gactgttccg tattgattgc gccccgctgg
     6301 ctgtctatcg ccgaacgcag gtagatttca tacatactgt catcgacggc attgcgtccg
     6361 accaggcgtt ttctgaagtt gttcagatat tgcgccgcct gaaccttggt cattttaccg
     6421 atacccacct gatagcccaa gcgcgtcgct tcatcgctga ttttggcaac atccgtccaa
     6481 tgcgaagagg caaggcggaa accttttgca ggtgcttccg ttttgacggt attgatagga
     6541 ttcacgggga tttccgtcaa tgtgggcaca taaatagact ggcagccgga aagaactgcc
     6601 acaatggaaa gagggataag gtattttttc atgcccccat tataatcaag tttgccttga
     6661 gaaaacaaat tgttcggcaa gaaaaataaa atttcggcat cagaagcagg caaaaacaca
     6721 ttccacaagc cttgccgcaa ggtttacaat ccgaccgtcc ttatcgcaac gaccgtttat
     6781 ggataccgca aaaaaagaca ttttaggatc gggctggatg ctggtggcgg cggcctgctt
     6841 taccattatg aacgtattga ttaaagaggc atcggcaaaa tttgccctcg gcagcggcga
     6901 attggtcttt tggcgcatgc tgttttcaac cgttgcgctc ggggctgccg ccgtattgcg
     6961 tcgggacacc ttccgcacgc cccattggaa aaaccactta aaccgcagta tggtcgggac
     7021 gggggcgatg ctgctgctgt tttacgcggt aacgcatctg cctttggcca ccggcgttac
     7081 cctgagttac acctcgtcga tttttttggc ggtattttcc ttcctgattt tgaaagaacg
     7141 gatttccgtt tacacgcagg cggtgctgct ccttggtttt gccggcgtgg tattgctgct
     7201 taatccctcg ttccgcagcg gtcaggaaac ggcggcactc gccgggctgg cgggcggcgc
     7261 gatgtccggc tgggcgtatt tgaaagtgcg cgaactgtct ttggcgggcg aaccctgctg
     7321 gcgcgtcgtg ttttaccttt ccgtgacagg tgtggcgatg tcgtcggttt gggcgacgct
     7381 gaccggctgg cacaccctgt cctttccatc ggcagtttat ctgtcatgca tcggcgtgtc
     7441 cgcgctgatt gcccaactgt cgatgacgcg cgcctacaaa gtcggcgaca aattcacggt
     7501 tgcctcgctt tcctatatga ccgtcgtttt ttccgctctg tctgccgcat tttttttggg
     7561 cgaagagctt ttctggcagg aaatactcgg tatgtgcatc atcatcctca gcggtatttt
     7621 gagcagcatc cgccccactg ccttcaaaca gcggctgcaa tccctgttcc gccaaagata
     7681 aaaaatgccg tccgaacatc cttcagacgg catatcgggc tttatttccc cgccttcaca
     7741 tcctgccact ggcgcaccat aaacttcaat gccgccggct ggataggcac catgataaag
     7801 ctgtttttca aatcctcctc ggttgggaaa atcgtattgt cgtttttaaa ttcgtcttcc
     7861 atcagctcac gcgcaggctt gctcgaaggc gcgtaagtaa cgaaattgcc gtttttcgcc
     7921 gacacttccg ggtcgaggaa gtcgttgatg tatttgtgcg cgttggcgac gtttttcgca
     7981 tctttcggaa tcacgaaaga atccacccaa atccccacgc cctctttggg catcatcacg
     8041 cggatttttt ccttgccgcc cgcttcttcg gcacggcgtt tggcgatgtt caaatcgccg
     8101 ccgaaaccga ttgttacgca ggtatcgccg cgcgccaaat catcgataaa gccggacgaa
     8161 gtaaagcgtt tgatattggg gcggtttttc ttgagtaggg cggttgcctc cctgatgtct
     8221 tccgtattgc tgctgttcgg gtttttaccc aaatagttca acaccatagg atagatttcc
     8281 gccgcgctgt ccaaatagct gatgccgcat tgcttgagtt tggacgtgta ttcggggtcg
     8341 aacaccaaat cccactggtt gtccggcagc ttgtccgtac ccaaagcctt tttcacgcgt
     8401 tcggtattga tggcgaaggt atttgtcccc caataaaacg gcacggcgta ttcgtggccg
     8461 ggatcgaccc cgtccatcag cctcatcatt tcgggattga ggtgtttata attgggaatc
     8521 agcgatttat cgattttctg atacgcacct gccttaatct gcctgcccac aaacgcattg
     8581 gacggcgcga caatgtcgta accggatttt ccggtcagca ccttgctttc cagcgtttca
     8641 tcgctgtcgt acacatcata agtaaccttg atgccgtttt tcttttcaaa atcggcaacg
     8701 gtttccggat cgacgtattc cgaccagttg taaattttca atacgtttcg gttttccgcc
     8761 ggcgccggtt tttcggcagg cggtttgtcc gaaccgccgc acgctgcaag cagcaaagca
     8821 gtcaggacgg cgagtggcag atgtttggcc attatcattc cttgcatatc gggttggaga
     8881 aagcggccat tatagccgat attgggaaca gggcttcaga cggcattcaa aatcccgcaa
     8941 tgccgtctga aagccgccgc ttccatagct agaaacaggg atttgcggta agataccgcc
     9001 gttcgttttc cctgctttta ccatgacaag acatttgaga gacattgaaa aaattatgaa
     9061 aacctccgaa ctgcgccaaa aattcctaaa attttttgaa accaaaggcc acaccgtcgt
     9121 ccgctcttcc agcctcgtgc cgcacgacga cccgaccctg ctgtttacca acgcgggcat
     9181 gaaccagttt aaagacgtat tcttaggttt cgacaaacgc ccgtacagcc gcgccaccac
     9241 cgcgcaaaaa tgcgtacgcg caggcggcaa gcacaacgac ttggaaaacg tcggctacac
     9301 cgcccgccac cacaccttct ttgaaatgat gggcaacttc tccttcggcg actacttcaa
     9361 acgcgacgcc atccacttcg cttgggaatt tctgacttcc cccgaatggc tcaacatccc
     9421 taaagacaaa ctgttggcga ccgtttacgc ggaagacgac gaagcctaca acatctggtt
     9481 gaacgaaatc ggtatgccgt ccgagcgcat cgtccgcatc ggcgacaaca aaggcgcgaa
     9541 atacgtgtcc gacaacttct ggcaaatggg cgacaccggc ccttgcggcc cctgctccga
     9601 aattttctac gaccacggcg aagaaatctg gggcggcatt cccggcagcc ccgaagaaga
     9661 cggcgaccgc tggatcgaaa tttggaactg cgtatttatg cagttcaacc gcgacgaaca
     9721 aggcaatatg aatccgctgc ccaagccgtc cgtcgatacc ggtatgggct tggaacgtat
     9781 ggccgccgtc atgcagcatg ttcacagcaa ctacgaaatc gacttgttcc aagacctgct
     9841 caaagccgtt gcccgcgaaa ccggcgcagc tttcagcatg gatgaaccca gcctgaaagt
     9901 catcgccgac cacatccgct cctgctcgtt cttgattgca gacggcgtgt tgccttccaa
     9961 cgaaggccgc ggctacgtat tgcgccgcat catccgccgc gccgtgcgcc acggttacaa
    10021 actcggtcag aaacaagcat ttttctacaa gctcgtgcct gatttggtga aagcgatggg
    10081 cgacgcgtat cccgagttga aagagaagca gacgcaaatt atggaagccc tgcgcgcgga
    10141 agaaagccgc ttcggcgaaa cgctggaaaa aggcatgggc ttgttcaacc aagtctataa
    10201 tgaattgaaa ttcaatcaaa tttttgatct tctggcgaaa gaatttaagg ttacacctgt
    10261 tgaagatatt gatcttgata tgtttagtac aagaaatttt gtaggaagaa gtgctgaaat
    10321 tccttatata tcaaatcaac accctaacgt ggattatccc gctcctatgg ggaacgtaaa
    10381 tgaaaaaatt tatgctatgc cagtgagatt aacagatgga agaattggta ttaatatttt
    10441 tcctactgat gaaactgcac gcctaatcct tagcaatgat tggaatagtt tgcgcttcta
    10501 ttctttttgt aattcgttga ttacttatta tcaacagaaa ggtcaattag aaacattaaa
    10561 attgaacggc gaacacatct tcaaactcta cgacacctac ggtttcccct acgacctgac
    10621 tgccgacatg gcgcgcgaac tgggcatcga actggacgaa gcaggcttcg agcgcgaaat
    10681 ggaagcccaa cgcgcacgcg cacgcgccgc ccaaagcttc aaagccaacg cccaactgcc
    10741 ttatgacggt caagacaccg agtttaaagg ttatagcgaa cgccaaaccg aatccaaagt
    10801 cctcgccctc tacaaagacg gcgagcaagt caacgaattg aacgaaggcg acagcggcgc
    10861 agtcgttatc gactttaccc cgttctatgc agaatccggc ggccaagtcg gcgacgtcgg
    10921 ctatatcttc tcaggcgaaa accgctttga agtacgcgat acccaaaaaa tcaaagcggc
    10981 cgtattcggc caattcggtg tacaaacctc aggccgtctg aaagtcggcg acagcgttac
    11041 cgccaaagtg gacgacgaaa tccgcaatgc caatatgcgc aaccacagcg cgacccacct
    11101 gatgcacaaa gccctgcgcg acgtattggg cggacacgtc gaacaaaaag gctctttagt
    11161 taccgccgaa tccacccgtt tcgacatttc ccatccccaa gcggtcactg ccgaagaaat
    11221 cgccgaagtc gaacgccgcg tcaacgaagc cgttttggcg aacgttgccg tcaatgcagc
    11281 cattatgagc atggaagacg cgcaaaaaac cggcgcgatg atgctcttcg gcgaaaaata
    11341 cggcgaagaa gtgcgcgtac tgcaaatggg cggtttctct accgaattgt gcggcggcac
    11401 acacgtttca cgcaccggcg acatcggcct cttcaaaatc atcagcgaag gcggtattgc
    11461 cgcaggcgtg cgccgtatcg aagccatcac cggcctgaac gcactcaaat gggcgcaaga
    11521 gcaagagcgt ttggtgaaag acattattgc cgaaaccaaa gcccaaaccg aaaaagacgt
    11581 actggcaaaa atccaagcag gcgcggcaca cgccaaagca ttggaaaaag aattggcacg
    11641 cgccaaagcc gaactcgccg tccacgcagg cgccaaactc ttggacgatg caaaagactt
    11701 gggcgcagcc aaactcgttg ccgcccaaat cgaagccgac gcagccgccc tgcgcgaaat
    11761 cgttaccaat ttaaccggta aatccgacaa cgccgtgatt cttttagcgg cagtaaacga
    11821 cggcaaagtc tccctgtgcg ccggcgtatc caaagccctg actggaaaag tgaaagcagg
    11881 cgacttggtc aaattcgcag ccgaacaagt cggcggcaaa ggcggcggcc gtcctgactt
    11941 ggcgcaagcc ggcggcacgg atgccgacaa attgcccgcc gtgttggata gcgtgaaaga
    12001 ctgggtcggc gcgaagctgg tttgatgtgg gaaaggcagc ctgaaaggtt tcaggctgcc
    12061 ttttgtgcaa agaggccgtc tgaaaggtct cgtttgccgt aggttgggtc gcgacccaac
    12121 aaattttgtg aagtataaaa atgttggtca tgacccaacc tacctgcctt tttgtacaaa
    12181 gaggctatct gaaaggcctt gtttgccgta tggtgggtcg cgacccagca gatttttatt
    12241 agggtatgac ccaagctact tgctacgata aaaaaggatt tttaaatgag cattagcctt
    12301 attggactac acattaccat agcaatcatt ttgtttttta ctacaaattt tatgggaaaa
    12361 aaatcatcta tatttggcta ttaccaactg tcttttagcg aagaaaatca ctctccggca
    12421 tttaatattt tttacagagc atttacccct atattattta tcgttatttt ttcttgggtt
    12481 gttactagtc ttgaaattcc catttctctt gaaaagataa actatgtagt aatttattat
    12541 tttataatta gattgttatc tgtatttgtt tttgagaaaa cacacatagt taactggttt
    12601 aatcaactaa caatacccat actatccata acattatcat ttatagtata taacaaaatg
    12661 attttgccca aaagttttct acttccatcc tcacaagaag tagctactac tttttgaata
    12721 gcgcttggtg gttacatata taatatatta aataatgaat cagggcattt aaaatcttat
    12781 aaagaaagaa gagtaaatta tgtaaaacac atgcacaaaa aatttgaaag ttattttggt
    12841 aaaattatag ataaaataat caaagaggat agttataata atgatgattt tttaaccgat
    12901 aagaaaaaag cactaatata ttcagtttta atttatgaga attttaatag gggactagtt
    12961 tatagatatt ttgaaaaaaa ttattttgta ctggtagaat aaaaacattt ggaataatgc
    13021 aagtaacctc agcagagtac ctttccaatg aggaaagtat aaaaaaaggc ggaaatattc
    13081 ttatggaaaa atacaatgaa aaatataatg aatctattga tggcaataaa actctctata
    13141 aatcatatta tgaatcaaga agagagagta ttaaaaacta caacccagat gcaaaataca
    13201 ttaatgaaat tgaatcaatt tacatgatgc ttggagaaat ctatccaaat gcaccagact
    13261 tcatgtcacc acattttgag ggggactgct ctgaggggga ataaaatcat tatttattct
    13321 ttattagtta ttagcaggat ttgtcgggca taaatgcccg acctacaaat tcaatttttt
    13381 caaacctctg ccaaatattt tcatctttgc aaggctgtct gaaaacccaa accccatttt
    13441 cagacggcct tttttcgcta aaatccccat accgttcaat ccgaaaacac aggagaatca
    13501 tcatggaagt taccatctcc gccatcatca atggcgaatt tgccgaccaa tacggcaagc
    13561 gcggtagtca gtttaatgaa aacgggatgc tgatttaatt ctatttcctt tgaaactacc
    13621 aataacctgc ctccatcata aaactaaaag caagccgtag cctgcattcc cacaaaccgc
    13681 gtgcgttgcc atgtcacaca ccctacctgc gggcgacgca aaccttaaga gacctttgca
    13741 aaattcccca aaatccccta aattcccacc aagacattta ggggatttct catgagcacc
    13801 ttctttcaac aaaccgccca agccatgatt gccaaacaca tcgaccgttt cccgctattg
    13861 aagttggacc aggtgattga ttggcagccg atcgagcagt acctgaaccg tcaaaaaaac
    13921 cgttacct
//
LOCUS       DAWHRS010000040        13889 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWHRS010000040 DAWHRS010000000
VERSION     DAWHRS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.040"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(92..1417)
                     /locus_tag="WIB82_000844"
     CDS             complement(92..1417)
                     /locus_tag="WIB82_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ3842164.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDLAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1628..2467
                     /locus_tag="WIB82_000845"
     CDS             1628..2467
                     /locus_tag="WIB82_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ3842165.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIIVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            2464..3630
                     /locus_tag="WIB82_000846"
     CDS             2464..3630
                     /locus_tag="WIB82_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ3842166.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGA
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRIHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3894..5159
                     /gene="efeB"
                     /locus_tag="WIB82_000847"
     CDS             3894..5159
                     /gene="efeB"
                     /locus_tag="WIB82_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ3842167.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYNYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5229..5558)
                     /locus_tag="WIB82_000848"
     CDS             complement(5229..5558)
                     /locus_tag="WIB82_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ3842168.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAVLQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNISCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5633..7003)
                     /gene="glmU"
                     /locus_tag="WIB82_000849"
     CDS             complement(5633..7003)
                     /gene="glmU"
                     /locus_tag="WIB82_000849"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ3842169.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDASAA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEIELGDNVEIGASCVIKNAKIGANTKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     ARLADDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(7058..7330)
                     /locus_tag="WIB82_000850"
     CDS             complement(7058..7330)
                     /locus_tag="WIB82_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ3842170.1"
                     /translation="MRKTFLILMTATALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7356..8036)
                     /locus_tag="WIB82_000851"
     CDS             complement(7356..8036)
                     /locus_tag="WIB82_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ3842171.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMAHRLKLSES
                     AASDLRQEYWHRYGATLAGLQIHHPEIDIADFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVWFGAKSHALPFIDACARDMAQLARYAETLSEH
                     RQNHYNTP"
     gene            8095..9096
                     /locus_tag="WIB82_000852"
     CDS             8095..9096
                     /locus_tag="WIB82_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3842172.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9190..10038
                     /locus_tag="WIB82_000853"
     CDS             9190..10038
                     /locus_tag="WIB82_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ3842173.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10065..10550
                     /locus_tag="WIB82_000854"
     CDS             10065..10550
                     /locus_tag="WIB82_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ3842174.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10652..12220)
                     /gene="msrAB"
                     /locus_tag="WIB82_000855"
     CDS             complement(10652..12220)
                     /gene="msrAB"
                     /locus_tag="WIB82_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ3842175.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGNI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12366..13631
                     /gene="ftsY"
                     /locus_tag="WIB82_000856"
     CDS             12366..13631
                     /gene="ftsY"
                     /locus_tag="WIB82_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ3842176.1"
                     /translation="MFSFFRRKKKQETPAPDEAQIQETAAKAESEVAQIVENIKEDAE
                     SLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETIS
                     EAVGQVKEDVATTEEHKLSWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2968 a         3536 c         4179 g         3206 t
ORIGIN      
        1 gaaaggaatt ttgtgtctgc ggtatcgcaa aacttcgcct taatgcgccc gattgcctag
       61 ggatgggctt cagatggcat tgttttccgg tttacgggcg gtattcgggc ttcataccgt
      121 tgggcagaag ctgccagaca tatcccgtgg ttttctgttt gccggcaagt tcgccggctt
      181 cgtcgccgta tccccaaaaa taatccacgc gcaccgcgcc tttaattgcg ctgccggtat
      241 cctgcgccat aatcaggcgg ttgagggctt tgcgggtaac cggatgggcg gtggcgacaa
      301 ataagggcgc acccaaggta atgtagtgcc ggtcgactgc gccggcatat tcccccatca
      361 gcggcgtgcc cagtgcgccg acagggccgt cattgccgct tccggtaagc tcgcggaaaa
      421 agatatagct ggggttttga cccaaaactt cggcgaggcg ttgcggattt tgccgcatat
      481 aagacttaat gccctgcatg gaggtttgtc cgagtttgag gtagccctta tccgccatgt
      541 attttccgat ggaaacatac ggatgctcgt ttttgtcggc atagccgatg cggatgtatt
      601 tgccggacgg ggttttcaga cggcccgagc cttggatgtg cataaaaaaa agttcgacag
      661 ggtcttcggc gtaaccgagt atcggggctt tgccgtcaag cgcgccgccg ttgatttggt
      721 tgcgcgtgtg gtaggggagg aagcggcttc cttcaaacct gcctttgatt gccgttgtgc
      781 gcgcggtgat ggggaatcgg gagaggtcgg cggtatgtgt gccgccggta ttgtcgattg
      841 tgccgctgtt tttccccgtc tgcctgatgc ggacaagggc ttttccgctc cgcaaaccgg
      901 caggcagggg gacggagata aaatcgtcgg gaataccgta aatcgggaag cgggcttgtg
      961 ccgtccgcct gtcgtcgccc ttcagcaccg gttcgtaata gccggtaacc gtaccggcaa
     1021 ggcttccgtt gcctgcaacc tgccacggcg tgaaatagcg ttcaaaaaac tgttttgcct
     1081 gaaaggaatg gacgggggtt tgaaaggctt gggcgcacac atcctgccag ccttggcggt
     1141 ttttcaaatt ggcgcagccg aggcggaagg attgcaggct tttggcgaaa tcctgcgccg
     1201 cccagtgggg cagggacagg tgcggtacaa cggtatagac ggccccgccg ccgccgaccg
     1261 tcgttccggc gaggtcgggg atgccgaccg gccggtccgg gccgttgatg acggatgtgt
     1321 cgggttgcgg aaaggtttgg atgctcttgc tttggcaggc ggcgaggatg gcggcggcga
     1381 tgccgtacag ggcggcgcgg aataggtatt ttttcataat ggacaatgtt gccggcagta
     1441 ataagaaaga tggtttcggg cggcgttgcg gcagccgtgg agaggggatt ttaacacagg
     1501 gcgcagctgc agcctgcgga actttccgcc gcgcggtact gcagataaaa ataacttgca
     1561 tttgtattta caagcaatga aaatattccg ataatatatt attcatcatc cttgttcgtt
     1621 cgcgtttatg ctggtcgctt ttttaattat gttgcgcgag ggtatcgaag ccgcgctcat
     1681 cgtcggcatc gttgccggtt ttctgaaaca gtccggacat tccaaactga tgcctaaggt
     1741 ctggttcggg gttgtccttg cttctttgat gtgtttgggg ctggggtacg gcatccattc
     1801 ggcaacaggc gagattcccc aaaagcagca ggagttcgtc gtcggcatca tcggtttggt
     1861 tgccgttgcc atgctgactt atatgatttt atggatgaaa aaggcggcgc gttcgatgaa
     1921 gcggcagctt caggattctg tgcaggcggc tttgaaccgt ggcagcggcc aaggatgggc
     1981 cttggtcggt atggcgtttc ttgccgtggc gcgcgaaggt ctagagagcg tttttttcct
     2041 gcttgccgta ttcaaacaga gcccgacgtg gcagatgccg gccggtgcgg tagcgggggt
     2101 tttggctgcc gccgtgattg gcgcgttgat ttatcagggt gggatgcgcc tgaatctggc
     2161 gaagtttttc cgttggacag gggcgtttct gattatcgtt gccgctggtc tgcttgccgg
     2221 ttcgctgcgt gcgctgcacg aagcaggcat ttggaacgcg cttcaggaca ttgtgttcga
     2281 ctcatcaaaa tatttgcacg aagacagccc gttgggcgtg ctgctcggcg gatttttcgg
     2341 ctataccgac catccgacgc agggcgaggc cttggtttgg ctgctgtacc ttattcccgt
     2401 cataacttgg tttttgtgcg gcagcaggcc gtctgaaact ttaacccgta aagaggagct
     2461 gaaatgagaa aattcaattt gaccgcattg tccgtgatgc ttgccttagg tttgaccgcg
     2521 tgccagccgc cggaggcgga gaaagctgcg ccggcagcgt ccggtgaggc gcaaaccgcc
     2581 aacgagggcg gtgcggtcag tatcgccgtc aacgacaatg cctgcgaacc gatggaactg
     2641 accgtgccga gcggacaggt tgtgttcaat attaaaaaca acagcggccg caagctcgaa
     2701 tgggaaatcc tgaagggcgt gatggtggtg gacgagcgcg aaaacatcgc ccccggactt
     2761 tccgataaaa tgaccgtcac cctgttgccg ggcgaatacg aaatgacctg cggccttttg
     2821 accaatccgc gcggcaagct ggtggtaacc gacagcggct ttaaagacac cgccaacgaa
     2881 gcggatttgg aaaaactgtc ccaaccgctc gccgactata aagcctacgt tcaaggcgag
     2941 gttaaagagc tggtggcgaa aaccaaaacc tttaccgaag ccgtcaaagc aggcgacatt
     3001 gaaaaggcga aatccctgtt tgccgacacc cgcatccatt acgaacgcat cgaaccgatt
     3061 gccgagcttt tcagcgaact cgaccccgtc atcgatgcgc gtgaagacga cttcaaagac
     3121 ggcgcgaaag atgccggatt taccggcttc catcgtatcg aatacgccct ttgggtggaa
     3181 aaagacgtgt ccggcgtgaa ggaaattgca gcgaaactga tgaccgatgt cgaagccctg
     3241 caaaaagaaa tcgacgcatt ggcgtttcct ccgggcaagg tggtcggcgg cgcgtccgaa
     3301 ctgattgaag aagtggcggg cagtaaaatc agcggcgaag aagaccggta cagccacacc
     3361 gatttgagcg atttccaagc caatgtggac ggttcgaaaa aaatcgtcga tttgttccgt
     3421 ccgctgatcg aggccaaaaa caaagccttg ttggaaaaaa ccgataccaa cttcaaacag
     3481 gtcaacgaaa ttctggcgaa ataccggact aaagacggtt ttgaaaccta cgacaagctg
     3541 ggcgaagccg accgcaaagc gttacaggcc tctattaacg cgcttgccga agaccttgcc
     3601 caacttcgcg gcatactcgg cttgaaataa gccgcaagcg ttcagacggt atttggcggc
     3661 agataccgtc tgaagtttta tagtggatta acaaaaacca gtacggcatt gcctcgcctt
     3721 gccttgccgt actatttata ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat
     3781 ccactatatc cgccatatat tgcagggcgg gatttcaacc tgccgctatc ggttaatgga
     3841 aaaacggcgt gcagggatac ccatcctgct gcacggatat tgaaggaaac accatgagca
     3901 aaaaccaacc cgcacaaccg accaggcgca ctctttttaa aaccgcgatc gcagccggcg
     3961 cagtcggcgc aatcggaggt tatctcggcg gcaaaaaaca gggcgaaacc gccgaacgca
     4021 ccgccgaaag ccaacactcg ccccaagcct atccctgcta cggcgaacat caggcaggca
     4081 tcgttacgcc gcagcaggcg ttttcgatta tgtgcgcctt cgacgtaacc gcgcaaagtg
     4141 ccaagcagct ggaaaacctg ttccgcacgc tgaccgcccg catcgagttt ctcacccaag
     4201 gcggcgaata ccaagacggc gacgacaaac ttccgccagc cggcagcggc attttgggca
     4261 aagccttcaa ccccgacggg ttgaccgtta ccgtgggggt gggcagcagc ctgtttgacg
     4321 gccggttcgg actcaaagac aaaaaaccga ttcatttgca ggaaatgcgc gacttctcca
     4381 acgataagct gcaaaaaagc tggtgcgacg gcgatttgag cctgcaaatc tgtgccttca
     4441 cccccgaaac ctgccaagcc gccctgcgcg acatcatcaa acacaccgtc caaaccgccg
     4501 ttatccgctg gagtatcgac gggtggcagc ctaaatccga acccggcgcg atggcggcgc
     4561 gcaacctgtt gggcttccgc gacggcacgg gtaaccccaa agtttccgac cccaaaactg
     4621 ccgacgaggt tttgtggacg ggggtggccg ccaacagcct cgacgaaccg gagtgggcga
     4681 aaaacggcag ctatcaggca gtccgcctta tccgccactt tgttgagttt tgggacagga
     4741 cgccgcttca agagcaaacc gacattttcg ggcggcgcaa atacagcggc gcgccgatgg
     4801 acggcaaaaa agaagccgat caaccggatt ttgccaaaga ccccgagggg aataccacgc
     4861 ccaaagacag ccatatacgc ctggcgaatc cgcgcgatcc cgagttcctt aaaaaacacc
     4921 gcctcttccg ccgcgcctac aactattcgc gcggactcgc ctcaagcgga cagcttgatg
     4981 tcgggctggt gttcgtctgt tatcaggcaa accttgcaga cggcttcatc ttcgtgcaaa
     5041 acctcctcaa cggcgaaccg ctggaagaat acatcagccc cttcggcggc ggctatttct
     5101 tcgtgctgcc cggcgtggaa aaaggcggct tcttgggaca agggctgctg ggcgtataaa
     5161 cctgcctgac ggacaaatgc cgtctgaatc cgataagggg ttcagacggc attttagtgt
     5221 ttgggcggtc aggctttttt gacgaattca gattttaaat tcatcgcgct gccgtcgatt
     5281 ttgcaggaga tgttgtgatc gccttcctgt aggcgtatgc ctttgacttt tgtaccttgt
     5341 ttgatcgtca tcgagctgcc ttttaccttg aggtctttga tgaggatgac ggtgtcgccg
     5401 ttttgcagca ctgcgccgtt ggcatcgcgc acttgagccg caaggtcggc ggcggattcg
     5461 gtttcattcc attcgtgggc gcattcgggg cagatgtatt gtccgccgtc ttcataagtg
     5521 tattcggagg cgcattgtgg gcatgggggt aatgacatgg tttgcagtcc ttatcggatg
     5581 tttgttttgg ggtaccgtct gaaacctgaa accggcttca gacggcatag ctttattgtt
     5641 tgtctttttc gggacgcacc cagccttcga tgacggtttg gcgggcgcgg gcgagggcga
     5701 gtttgttgtc ttcgatattg cgggtaatcg tgctgcctgc gcctgtggtt actttgttgc
     5761 ccagtgttac gggggcaacc agtacgcagt ttgaaccgat gcgcacttcg tcgccgatga
     5821 cggttttgtg tttgtgcacg ccgtcgtagt tggcaataat cgtaccggcg ccgaagttgg
     5881 ttttgctgcc gacttcggca tcgccgatgt aggtgaggtg gttggctttg gtgcctttgc
     5941 cgatggcggc gtttttgatt tcgacgaagt tgccgacgtg tacgtcgtct gcaaggcggg
     6001 cttgcggacg caggcgggcg tacgggccga ttcggttgtt ttcgccgact tcgcagtctt
     6061 cgaggtggga gaagggggcg attttggtgt tggcaccgat tttggcgttt ttgatgacgc
     6121 agcttgcgcc gatttcgacg ttgtcgccga gctcgatttc gccttcaaag atacagttca
     6181 catcaattac gacatcctgt ccgtgtttca gacggcctcg taaatcgaaa cgtgccggat
     6241 cgcgcagggt tacgcctgct ttgagcaatt cttgtgcctg ttcggtttgg aagatgcgtt
     6301 cgagttcggc gagctggcgt ttgttgttca cgccggcggc gaggtgggag gcgcgcactc
     6361 ggacgggatg aactttaata ccgtcggcaa cggctttggc gatgaggtcg gttaaataat
     6421 attcgccttg ggcattgttg ctggaaaggc tgttcagcca gttttccagt ttggcgttgg
     6481 gcaggacgag aatgcctgtg ttggtttctt tcacggcttt ttgggcggca cttgcgtctt
     6541 tttcttcgac aatggcggtt acgctgccgc tgccgtcgcg gatgatgcgc cccaaacctg
     6601 acgggtcggc gggcacgtcg gtcaacagcc cgacttcgtt gcctgcggct tcgagcaggg
     6661 tttcgagggt ttcaacgtca attaaaggaa cgtcgccgta caacaccagc gtgcggcctt
     6721 cggcggcaag gtggggcagg gcggttttga cggcgtggcc ggtgccgagc tgttcggttt
     6781 gttcaaccca gacgacatcg cgtttgacgg tgtctaaaac ttgctctttg ccgtggccga
     6841 cgacgacgca gatgttttgc ggattcagcg cggcggcggt gtcgataacg cgcccgagca
     6901 ttgtttcgcc gccgatttcg tgcaaaacct tgggcatttt ggaatacatg cgcgtgcctt
     6961 tgccggcggc gaggatgacg atgtttaaag tgttttgcgg catagcgttt tcctgtgcaa
     7021 tgccgtctga aagggcttca gacggcatag ggtgggttta tcggttttga aatttgggct
     7081 tttgccagtg ttggcgatgc tcttcgtcgg cgttgttgcc ggtttggttg ggtaacacgg
     7141 catggcgttc ggggcggtat tggttgtagt tcatattttt cgagtagctg ccgtcttggt
     7201 aataaacggg cgtgccggca ggatattttt gacggacggc ggttttgccg ttgccgtctt
     7261 gataagtttc ccacgcgcag cccgacaaaa gggcggtggc agtcatcagg ataaggaagg
     7321 ttttacgcat ggcttttctt tcgtattttc gggggttagg gggtattgta atgattttgg
     7381 cggtgttctg acaaagtttc tgcataccga gccagttgcg ccatatcgcg tgcgcaggca
     7441 tcgataaagg gcagcgcgtg ggattttgcg ccgaaccaga cggttttcat gcccaatgct
     7501 tttgcctgat gcaggttgtc cgcgctgtcg tccaccataa tgcagcattc gggcggtacg
     7561 tccaaaaggc ggcagacatt gagatacgct tgcggattgg gtttgtacag cagtccgaaa
     7621 tcatccgtgc cgaacagtgc gtcgaaacgg ttttccaaac cgagtgcgcc gacaacggca
     7681 cggacgtaaa acgacggacc gttggaaaaa accgccttgc gcccttttag gcggctcagg
     7741 gtgttttgtg tttcaggcat gccgtgcagc ctggtcagga ttgcatcgat cggatggctt
     7801 tcgcgcaaaa aatcggcgat gtcgatttcg ggatggtgga tttgcagtcc ggcaagcgtt
     7861 gcgccgtagc ggtgccaata ttcctgacgc aggtcggacg cggcggattc ggagagtttg
     7921 aggcggtgtg ccatatagcg tgtcatagcg cggttgatga gtgtgaagat gccggcatcg
     7981 gcattgtgca gcgtgttgtc gaggtcgaac agccacacgg tcgggttttc ttgcatgttg
     8041 aaccgtgaaa atttgttaga atgttatttt acagcgaata gaggaggact cggaatgaaa
     8101 cggaaaattt ggctgctgcc gctgctggcg gtttcggcat acctgcaggc gcagacggaa
     8161 gtcaggctgg cggtgcataa gtcgttcagc ctgcccaaag ggttgattgc gcgcttcgag
     8221 cgggcaaacg atgcgaaggt gtcgattatt caggcgggcg gcgcgaacga aatgctcaac
     8281 aaactgattt tgagccgcgc caacccgatt gccgacgcgg tgtatggttt ggacaacgcc
     8341 aatatcggca aggcgcggga aatgggcatt ttggcggcgg cgcaacccga atccgccccc
     8401 gtcgcggtcg ggctgccttc ggctttggcg gtcgattacg gctatgtgtc catcaattac
     8461 gacaaaaaat ggtttgaagg caaaaagctt cctctgccgc aaaccctgca ggatttgacc
     8521 cgccccgaat ataaaaacct attggtcgtg ccgtctcccg ccacgtcgtc cccggggctg
     8581 ggcttcctga tggcgaacat cagcggtctg ggcgaagaag gcgcgttcaa atggtgggca
     8641 cagatgcggc agaacggcgt gaaggtcgcc aaaggctgga gcgaggcgta ttacaccgac
     8701 ttttcgcaca acggcggcgc gtatccgctg gtggtcggtt atgccgccag cccggcggcg
     8761 gaagtgtatt tttccaaagg caaatacagc gagccgccga cgggcaacct gtttttaaaa
     8821 ggcggcgtat tccgccaggt cgaaggcgcg gcggtcttga agggcgcgaa acagccggaa
     8881 ttggcggcaa aactggtgca atggctgcaa agtcgggaag tgcagcaggc ggttccgtcc
     8941 gaaatgtggg tttaccccgc cgtcaaaaac acgcgcctgc ccgacgtgtt ccgcttcgcc
     9001 caagccccga cgcacaccac cgcccccgcg cagcgcgata ttgatgcgaa ccagcgcgga
     9061 tgggtttccc gttggattag aacggttttg aaataaaaca aacataccgc cccgcagggc
     9121 ttcatacggc atttttacac ctgtgccgat tacgccgcac ggggcggatg ttcgatcaag
     9181 aggaaaacaa tggacttcaa acaatttgat tttttacacc tgatcagtgt ttccggttgg
     9241 gagcatctgg ctgaaaaggc gtgggcgttc gggctgaacc ttgccgccgc gctgcttatt
     9301 tttttggtcg gaaaatgggc ggcgaaacgc attgtcgctg tgatgagggc ggcgatgacg
     9361 cgcgcgcagg tcgatgccac gctgattagt tttttgtgta atgttgccaa tatcggctta
     9421 ttgattttgg tgattattgc cgcattgggc agattgggcg tttccacaac atccgtaacc
     9481 gccttaatcg gcggcgcggg tttggcggtg gcgttgtcct tgaaagacca gctgtccaat
     9541 tttgccgccg gtgcgctgat tatcctgttc cgcccgttca aagtcggcga ttttatccgc
     9601 gtcggcggtt ttgaaggata tgtccgagag attaaaatgg tgcagacttc tttgcggacg
     9661 accgacaacg aagaagtcgt gctgcccaac agcgtggtga tgggcaacag catcgtcaac
     9721 cgttccaccc tgccgctttg ccgcgcccaa gtgatagtcg gcgtcgatta caactgcgat
     9781 ttgaaagtgg cgaaagaggc ggtgttgaaa gccgccgtcg aacacccctt gagcgttcaa
     9841 aacgaagagc ggcaggccgc cgcctacatc accgccttgg gcgacaatgc catcgaaatc
     9901 acattatggg cttgggcaaa cgaagcagac cgctggacgc tgcaatgcga tttgaacgaa
     9961 caagtggtcg aaaacctccg caaagtcaat atcaacatcc cgttcccgca acgcgacata
    10021 cacatcatca attcttaaac gccgtctgaa aggggagtgg gaaaatggac gcgctgcaca
    10081 ccatcgcccg aaacctgacg aaaaaacgtc aaaccgtaag ctgtgccgag tcctgtacgg
    10141 gcggaatgct tgccgccgca ttcacaagcg ttgcaggcag ttcgcaatgg ttcgaccaga
    10201 gttttgtaac atacagcaac aaagccaaag aagaccgctt gggcgtgttg cccgaaaccc
    10261 tgctcgaaca cggcgcggtc agccgccaaa ccgtctatga gatggcgcgc ggcgcgaaag
    10321 ccgtggcgca ggcggattac gccgtcggta tttccggcat cgccggaccg ggcggcggca
    10381 gcgaaagcaa acccgtcggc acggtttggt tcgggtttgc ctttccgggc ggaagttgcg
    10441 aagcaatgcg ccgttttgac ggcaaccgcg aatccgtccg cgcgcaggcg gtcgccttcg
    10501 cgttggaacg gttggcgggg ctgattgaaa acggcggcga tgctgtctaa acaaaatctc
    10561 cgtctggaca gaatccccgc cggataaaaa ataccgtctg aaacgttgca ggtttcagac
    10621 ggtattttgt cgggttaggc ggcggtgcgg cttatttcac tttactcttc aacgcgccgt
    10681 agcctgccgc gtccatttgt tccagcggga tgaatttcaa gctcgcgccg ttgatgcagt
    10741 agcgcagtcc gcctttgtcg cgcgggccgt cggggaagac gtgtcccaaa tgcgaatcgg
    10801 cggcgtggct gcgcacttcg gtgcggcgca tgttgtagct gaaatcatcg tgttcggtaa
    10861 cggattttgc atcaatcggg cgcgtgaagc tcggccagcc gcagccggaa tcatatttgt
    10921 cggcggagct gaacaaaggt tcgccgctga caacgtccac ataaatgccg ggtttgaaca
    10981 aatggtcgta ttcgtggctg aaggcatatt cggtcgcgct gttttgggta acttggtatt
    11041 gctcttcggt cagggtgcgt ttgagttcgg cgtcactcgg ttttttatac gttgccgcgt
    11101 cgaagccttt gccttgcggg gcggtcttgg ttttgcccgg cagcggttcg tcggctttgc
    11161 ggatatcgat gtggcagtag ccgttggggt ttttaatcag gtagtcctga tggtattcct
    11221 cggcatcgta gaagtttttc agcggctcgt tttcaacaac gaggggcagt tggtattttt
    11281 gctgctcgcg tttgagggcg gcggcgatga cggctttttc ggcggggtcg gtgtagtaca
    11341 cgccgctgcg gtattgcgta ccggtgtcgt tgccctgttt gttgaggctg gtcggatcaa
    11401 cgacgcggaa gaaatattgc aggatgtcgt ctagactgag tttgtcggca tcgtaggtca
    11461 ctttgacggt ttcggcgtgg cccgtatggc ggtaggacac gtcttcatag ctcggatttt
    11521 tcgtgttgcc gttggcgtag ccggataccg cgtcaaccac gccgtcgatg cgttggaaat
    11581 aggcttccaa gccccagaag cagccgccgg cgaggtagat ggtgcgcgtg ttcatgattt
    11641 ttgaatcctt tttctgagtg tcgggtttgt agaacgaatg tttcaagctg cccaaatcgg
    11701 cattcgggtc gcggattaac gccaacgcct gcgcttcgtt gatgttgcct ttgacgatgc
    11761 gctgcacgtc gccgtcttta ccgattaacg cccacgaggg gtaaacgctg atattcaggc
    11821 tttgggcgat tgtgccgccg ttgtcggtta cgacgggcag cttgggataa ttcaaaccgg
    11881 cataccattt ttggaagtcg ccgtcttttt tctcgtgcaa aaagcccgga gacgcgacgg
    11941 taatcaggtt ggcggagctg aattttgcat cttgcgccca tttttcggtc tgtcccaatt
    12001 cggacagaca caaaggacac cagctcgccc aaaatttaat cagcgtcggt ttgtcttttt
    12061 tcaagtaaac actggcgggg cggttgtctg cggttttcaa agtggataaa gtgtgcggca
    12121 cggtcgcggc tccggcatcg acgattttgg gcgaacaagc acccagcgca agcaggcagc
    12181 cgaacttggc gcaaagggaa aagaaagtac ggtgtttcat tttgatgttt cctgtgtgga
    12241 cggtttgcat gattagacgt ttgagatgcc gaaaccttac agcccggatt ttcagacaac
    12301 cttgccgcgt aaaatacgct acaatactcc ctatttcaag tttctaaaat taaaaggaaa
    12361 attcaatgtt cagcttcttc cgtcgcaaga aaaaacagga aactccggct cccgatgagg
    12421 ctcaaattca ggaaaccgca gcaaaagcag agtctgaagt tgctcaaata gttgaaaata
    12481 ttaaagaaga tgctgaatct ttaacggaaa gcgtcaaagg gcaggtcgaa tctgccgttg
    12541 aaaccgtcag cggtgcggtt gaacaggtaa aggaaaccgt tgccgagatg ctgtctgaag
    12601 cagaggaagc ggcggaaaaa gcagcggaac aagtcgaagc ggcgaaagaa gctgttgccg
    12661 aaaccatcag cgaggctgtc gggcaagtta aagaagacgt tgcgacaact gaagaacaca
    12721 agctcagttg ggcggcgcgt ttgaaacaag gtttgaccaa atcgcgcgac aaaatggcga
    12781 aatcgctggc gggcgtgttc ggcggcggac aaatcgacga agatttgtac gaagagctgg
    12841 aaaccgtgct gattaccagc gacatgggca tggaagccac cgaatacctg atgaaagacg
    12901 tgcgcgaccg cgtcagcctc aaagggctga aagacggcaa cgaattgcgc ggcgcgttga
    12961 aagaagcctt gtacgacctg attaagcctc tggagaaacc tttggttttg cccgaaacca
    13021 aagagccgtt tgtcatcatg cttgccggca tcaacggcgc gggcaaaacc acgtctatcg
    13081 gcaaactcgc caaatatttc caagcgcagg gcaaatccgt attgctggcg gcaggcgata
    13141 ctttccgcgc cgccgcgcgt gagcagcttc aagcttgggg cgaacgcaac aacgttaccg
    13201 tgatttcgca aaccacgggc gattccgccg ccgtgtgctt cgatgccgtc caagccgcca
    13261 aagcgcgcgg catcgacatt gtgctggccg acaccgccgg ccgcctgccc acgcagcttc
    13321 atttgatgga agaaatcaaa aaagtgaaac gcgtgctgca aaaagccatg cccgacgcgc
    13381 cgcacgaaat catcgtcgtg cttgatgcca acatcgggca aaacgccgtc aaccaagtca
    13441 aagcctttga cgacgcatta gggctgactg gtttaatcgt gaccaaactc gacggcacgg
    13501 caaaaggcgg catcctcgcc gcgcttgcct ccgaccgccc cgttcccgtc cgctatatcg
    13561 gcgtgggcga aggcatagac gacctgcgcc cgtttgacgc gcgcgcgttt gtggacgcac
    13621 tgctggattg agccgaaatg ccgtccgaaa acagcagacc gatgccgtca ttcccgcgca
    13681 ggcgggaatc cagaccttgg gataacggca atattcaaag gttatctgaa agtccgagat
    13741 tctggattcc cactttcgtg ggaatgacgg gatgtaggtt cgtgggaatg acgtggtgca
    13801 ggtttccgta tggatggatt cgtcattccc gcgcaggcgg gaatctagaa cgtaaaatct
    13861 aaagaaaccg tgttgtaacg gcagaccga
//
LOCUS       DAWHRS010000041        13877 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWHRS010000041 DAWHRS010000000
VERSION     DAWHRS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13877
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.041"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            20..748
                     /gene="rpe"
                     /locus_tag="WIB82_000857"
     CDS             20..748
                     /gene="rpe"
                     /locus_tag="WIB82_000857"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ3842177.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WIB82_000858"
     CDS             complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WIB82_000858"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ3842178.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDHFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNIAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1884..2192)
                     /locus_tag="WIB82_000859"
     CDS             complement(1884..2192)
                     /locus_tag="WIB82_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842179.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            complement(2405..3082)
                     /locus_tag="WIB82_000860"
     CDS             complement(2405..3082)
                     /locus_tag="WIB82_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HEZ3842180.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
     gene            complement(3167..4420)
                     /locus_tag="WIB82_000861"
     CDS             complement(3167..4420)
                     /locus_tag="WIB82_000861"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ3842181.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYAGFSFHTDKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVEKLRSKTIIDVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            complement(4492..6120)
                     /locus_tag="WIB82_000862"
     CDS             complement(4492..6120)
                     /locus_tag="WIB82_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ3842182.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            6270..6623
                     /locus_tag="WIB82_000863"
     CDS             6270..6623
                     /locus_tag="WIB82_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ3842183.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEISL
                     WLEQVPLEILLNKRGTTWRKLNALMQEEALSSKEGAVRIMSEMPSLIKRPVLEYGGKV
                     HVGFSDESYREIFKR"
     gene            6703..8241
                     /locus_tag="WIB82_000864"
     CDS             6703..8241
                     /locus_tag="WIB82_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ3842184.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEQSLNAAVQNEQADGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            8308..8925
                     /gene="recR"
                     /locus_tag="WIB82_000865"
     CDS             8308..8925
                     /gene="recR"
                     /locus_tag="WIB82_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ3842185.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(8981..9259)
                     /locus_tag="WIB82_000866"
     CDS             complement(8981..9259)
                     /locus_tag="WIB82_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842186.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            9464..10711
                     /locus_tag="WIB82_000867"
     CDS             9464..10711
                     /locus_tag="WIB82_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ3842187.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            10704..11399
                     /gene="lolD"
                     /locus_tag="WIB82_000868"
     CDS             10704..11399
                     /gene="lolD"
                     /locus_tag="WIB82_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ3842188.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIHAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            11467..13212
                     /gene="recD"
                     /locus_tag="WIB82_000869"
     CDS             11467..13212
                     /gene="recD"
                     /locus_tag="WIB82_000869"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ3842189.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(13240..>13877)
                     /locus_tag="WIB82_000870"
     CDS             complement(13240..>13877)
                     /locus_tag="WIB82_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3842190.1"
                     /translation="PQLGGSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSG
                     VLAAILAKQGIPSVIGTDTNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVC
                     NPPWLPAKPTSAVESALYDPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPS
                     DFLDKAFAQAGLRVADMMKTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
BASE COUNT         3114 a         3528 c         3978 g         3257 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcgggccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gcgtgccgat cgatgtgcat ctgatggtcg aacccgtgga
      241 tgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat attgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtaa accccgggtt cggcggtcag agcttcattc cgtatacttt
      481 ggaaaaaatc agacaggttc gtgccatgct tgaccgctat gaagagaaaa gcggcagacg
      541 tattgccatt gaagtggacg gcggcatcaa aaccgacaat atcgccgccg ccgcccaagc
      601 cggcgcggat acctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatagaa atgccgtctg aaaattagct ctgtttcaga cggcattgcg ttatttttcg
      781 acgggcagcc cgaaatgcag gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt
      841 tgcaggaagc cttgttggat aaggtagggt tcgataacgt cttcgatggt gtctgtagat
      901 tcgccgatgg cggcggcaat attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa
      961 acggcttcga gaaatttcct gtccatcacg tccagcccct gcgcgtccac gtccagcata
     1021 cttaaagcgg catcggcgat gccgccgtcg attgtgccgt tgtttttcac gtcggcgaaa
     1081 tcgcgcacgc gtcgcaacag gcggttggcg atgcgcggcg taccgcggct gcgtttggcg
     1141 atttcttccg cgccttcttc ggacatatcg agctgcaaca gttgtgccga acggctgacg
     1201 atggtggtaa ggtctcggtt ttcgtaaaac tcaaggcggg agacgatgcc gaagtggtcg
     1261 cgcaacggat tggtcagcat accggcgcgg gtggtcgcgc cgatgagcgt gaagggcggc
     1321 aggtcgattt tgacggaacg ggcggcgggt ccttcgccta tcataatgtc gagccggtag
     1381 tcttcgagcg cgggatagag gatttcttcg acaacagggc tgaggcggtg gatttcgtcg
     1441 atgaacaata catcgtgcgg atcaaggttg gtcaaaaggg cggcgaggtc gcctgcgcgt
     1501 tcgaggacgg ggccgctggt ttggcgcaaa tttacgccca attctttggc gatgatgtgc
     1561 gccagtgtgg ttttgcccag tccgggcggg ccgaagagca aaacgtggtc gagtgcttcg
     1621 ccgcgttttt tggcggcttg gatgaaaatg gcaagctgtt ctttggcttt gtcttgcccg
     1681 atgtagtcgt ccagcgtttt ggggcggagg gcgcgttcga gcagttcttc ctgtgcggag
     1741 gcggtttggg cggcaacgat gcgttgcggt tgcgcggcgg tcaggttgtc ggtttgcagc
     1801 atagtgttcc ctttgtcggg tatgccgtcc gaacggtcgg cggcgtttca gacggcattg
     1861 aaaagataac ggtcaaaagc gttttatccg cgggtttcgc gccaaacgag atagtcgcgc
     1921 aggggcggca taggcgggtt gatgcagtgg gggcagatgc cggtggtgtc ttcgtcgtcg
     1981 ctgtcgatgt ggtaggcgtt ggggtcgaag cggatttgct gcatgacggc ttgcgccaat
     2041 gcctgcgcct gaacaacgtc gaaattgaat ttttcgagga tttcgttcaa caggcggatt
     2101 tcgccaccgc taaattccgc actgtgtttg aggatgaggc gttgataatc ttcgttgttg
     2161 attttaggca ataaatcggt tttcagggtc atgattttat gtcgggggcg gaatggctga
     2221 agcgtgaatt atagcggata tggcggcggc tgttgcaaag cttggattgg gtatcgtcct
     2281 caaaacttta tagtggatta acaaaaacca gtacagcgtt gcctcgcctt gccgtactat
     2341 ctgtactgtc tgcggcttcg tcgccttgtc ctgattttta ttaggggctt aggaagcaaa
     2401 aatattaaat tgggttattt cgaagcatct tcattaatat tttacctaaa tcttcatgcc
     2461 ggtggttaaa cctaaactcc gtttctttga gatgcagata aaacatctct ttggaaatac
     2521 catgaaattt agccaaacgc cccttagtat aactccaaaa agattcaata ccattgatat
     2581 gttgctttcc tcgagcaaac tcattggaac catgatggac acggtaatgc ttctcatagc
     2641 ccatatcgac aagaccgtca tatgctttcc agccgtcagt attgatttca ctgtcagcag
     2701 atatgtgacc acgtatgacc ttgattagtg aggctttcga agcgtcggga acgatttccg
     2761 tatagaccac gccattgcgt ttcagtatgc caaatacgat ggttttacct gacgctccgc
     2821 gaccgcgctt acccctgata cgtcgcgcac caaagtaaga ttcatctaat tcgaccacac
     2881 cggacagtgg tgagctttgt tcgcacagag cagcaatcct gtgtctgatt ttcaggaaaa
     2941 taggattgat accgcgtaca ctaatgccgg tcattttagc agtatcggaa gcggtcaaat
     3001 caagagcgaa acagcggatt atttctctaa acttttgctc tgaaattttg ctgaactttt
     3061 gatacttatt ttttagtttc atattaggag cttatcaggt tttttgatga ttttgcttcc
     3121 taagcccctt tgttaatcca ctataaaagt ggctgcattg gacgttttaa tgtttttctg
     3181 ttttgtcttg cgccgctcga aacgcttttt gcaatggggc gatttgtgcc agccgcgccc
     3241 ggtcgatttg ttcggcgata aggtgcgctt ttccctgcgc gcggcattcg gcggcgattt
     3301 tgcccgaatc cgcctgattg gcggcttcga gtaaggcgag ccagtgcgcg cgttgcgggt
     3361 agggcgtgtg ttcgcggttc agacggcctt gcgtgtcggc aatgcagacg ttcaatgccg
     3421 tctgaaaacg ttctgggcgg cggaaggcat cggttttttt caatacgtcg ataatggttt
     3481 tgctgcggag tttttcaacc tgatggaaga tgatgtgcca acggcaaacc aattcggcaa
     3541 gctcggcgca atgtttcggc gcgcgcagcc gctgattgac ttcgcgcacg ggttcgacac
     3601 cggcgaggtc gtgcccgtgg tggcgcggca ggatgtcgga cggtgttttg gctttgccca
     3661 agtcgtgcag cagggcggca tagcgttcgg gcaggctcaa acccatatcg gcggcgcgtt
     3721 gcagcgtcat cagggtatgg atgccgctgt cgatttcggg atggtagtcg gcgcgttgcg
     3781 gcacgccgaa gagggcattg acttcgggca gcaagacttt gagcgcgccg cattcgcgca
     3841 acacttcaat cattttgcgc ggattttttt ccatcaaacc tttcgcaaac tcctgccaga
     3901 cgcgttcggc aaccaatgcg tccgcttcgc cgttttccac catctgccgc atcagcttta
     3961 tggtttcttc ggcgatttca aacttgtaac gcgcggcaaa gcgggcagta cgcaggatgc
     4021 ggacggggtc ttcggcgaag gccggggaaa cgtggcgcaa aatgcctgcc gccaaatccc
     4081 gttgtccgcc gaaagggtcg ataatcttgc cgtccgcatc ttgcgccatc gcgttgatgg
     4141 tcaggtcgcg gcgcatcaaa tcctgctcca gcgtaacgtc tttgtcggtg tggaaactga
     4201 agccggcgta acctttggcg gttttgcgct cggtgcgggc gagggcgtat tcttcgtgtg
     4261 tttcgggatg gagaaacacg ggaaagtctt tgccgactgg ctggaagcct tgcgccagca
     4321 tggtttgtgc gtctgcgccg acgaccaccc aatcgcggtc tttgacgggc aagcccaaaa
     4381 gataatcgcg gacggcaccg ccgacgagat aagtctgcat atgcttccta ttttaaagtt
     4441 atcaacaatg ccgtctgaag ccgcttcaga cggcattgtt ccaaccggcg gttatgctac
     4501 gccttttttc tcgagatagc tttcgtaatc gcccaagtag tgttcatatc cgcctttgcc
     4561 gtccaattca atgatttggg tagccaggga agaaacgaac tgacggtcgt gggagacgaa
     4621 aatcagcgtg ccgttgtatt tttccagcgc catgttcaag gattcgatgc tttccatgtc
     4681 catgtggttg gtcggttcgt ccatgaccaa gacattgggt ttcaacagca acagtttgcc
     4741 gtaaagcata cggccttttt caccaccgga gagaaccttc acttttttca cgacatcgtt
     4801 actgccgaag agcaaacgcc ccaaagtgcc gcggatgact tgttcgtcgt cgccgtcctg
     4861 cccccattgg cgcatccatt cgctcaaatc catatcgaca tcgaagtcgt tttcgtgatc
     4921 ttgcggatag tagccgacgc tggctttttc cgcccatttg atgctgcctt cgtccggcag
     4981 cagaccgtct gaatattcgg ggttgtacgc gccggccaag agtttcagca gggtggattt
     5041 gcccgcgccg ttcgggccga tgatggcgag gcgttgtccc gcttcaagga tgaagttcag
     5101 gtttttaaac aactgggttt caaagcgttt cgccagtttt tcaacttcca cagcctgacg
     5161 gtgcagcttg gctttttcat cggcttcaaa acggatatac gggttttgac gggtggaagg
     5221 tttgacttcg accatctccg atttgatttt gtcggcctgt ttcagacggc tggttgcctg
     5281 acgggctttg gatttgttgg cagagaagcg tgcgacaaac tcttgcagct cttgcagctt
     5341 ctctttggct ttggcgttgt ctttcagggc gcgctcacgc gattgggcag aagcgagcat
     5401 gtaatcgtcg tagttgcccg gatagatggt gatggtgttg tagtccaaat ccgccatatg
     5461 cgtgcagact tcgttcaaaa agtggcggtc gtggctgatg ataatcatcg tggagtcgta
     5521 ttggttcaac acgccttcca accagcggat ggtattaatg tccaagttat tggtcggttc
     5581 gtccaagagc aatacatccg gcttggagaa cagcgcctgc gccagcaata cgcgcagttt
     5641 gaagcccggg gcgacttccg ccattttcgc attgtgcaaa tcttcggaaa tgcccacgcc
     5701 gctcaacagt tcggcggcac gcgcttcggc ggtgtagccg tcgtattcgg cgaacttggc
     5761 ttccagttcg gcggctttca tgtagtcgtc ttcggtggct tcgggattgg cgtaaatcgc
     5821 atcacgttcg gtcatcgccg cccacatttc ggtatgcccc atcatcacca cgtccagcac
     5881 gcgcatgtct tcgtaggcaa actggtcttg gcgcaattta cccaaacgca cgccgttttc
     5941 aatcgccact tcgccggccg tctgttccaa atcgccgccg aggattttca tgaaggtgga
     6001 tttgcctgaa ccgttcgcgc cgatcaggcc gtaacggttg ccttctccga attttacgga
     6061 tacgttttca aacagcggct tcgcgccgaa ctgcatggtg atgccgttgg tagaaatcat
     6121 gattggtaaa gcctttattg aacaagagat taaatttttc gaattttcgg attgtaacat
     6181 aaccgccccg ccgtctgaaa cagacgggca aattttcaga cacttatagt aaaatatccg
     6241 ttttattatt tcagacaaaa ggtttccaaa tgatagtcct tcacggcatc ccaaattgcg
     6301 atacggtcaa aaaagccaaa aacaggcttg ccggatacgg cttggagttt gaatttcggg
     6361 atttcaaaaa acagaggccg tctgaagcgg aaatcagcct gtggctggag caggtgcctt
     6421 tggagatact gctcaacaaa cgggggacta catggcgcaa acttaatgca ctgatgcagg
     6481 aggaagccct gtcctcaaag gaaggtgcgg tcagaataat gtccgaaatg cccagcctta
     6541 tcaaacgtcc cgtactggaa tacggcggaa aggttcatgt cggcttcagc gatgaatctt
     6601 atcgagagat attcaaacgt tagttaagcc ttcgtgcagg cgcgtataaa aaccgttaca
     6661 atacccgact tgaatttccc attctatatc ccgatttaaa atatgttcca ttccatcgaa
     6721 aaatacagaa cgcccgccca agtcttatta ggcctgattg cattgacctt cgtcggcttc
     6781 ggggtcagca cggtatccca tccgggcgcc gactacatcg tccaagtggg cgacgaaaaa
     6841 atcagcgagc agtcgcttaa cgccgccgtc caaaacgagc aggcggacgg cggcagccct
     6901 tcgcgcgacg cggtgttcca atccctgcta caacgcgcct acctgaaaca gggcgcgaag
     6961 ctgatgggca tttcggtttc ttccgaacaa atcaagcaga ttatcgtgga cgatcccaat
     7021 ttccacgacg caaacggcaa attcgaccac gcgcttttaa accgctacct gtcgcaacgc
     7081 catatgtctg aagaccagtt tgtcgaagaa atccgcgatc agtttgcctt gcagaatttg
     7141 gtaaacctcg tccaaaacgg cgtattggtc ggcgacgcgc aggcggaaca gctgatcagg
     7201 ctgacgcagg tcaaccgcac catccgttcg cacactttca accccgacga attcatcgcc
     7261 caagtcaaag tgtctgaagc cgatttgcag aagttttata acgcaaacaa aaaagactac
     7321 ctgcttccca aagcggtcaa attggaatat gtcgcattga atctgaaaga ctttgcagac
     7381 aaacagaccg tcagcgaaac agaagtgaaa aatgcgtttg aagagcgcgt ggcgcgtttg
     7441 ccggcaaatg aagccaaacc ttctttcgag caggaaaaag ccgccgtcga aaacgaattg
     7501 aaaatgaaaa aggcggttgc cgacttcaac aaggcaaaag aaaaattggg cgacgatgcg
     7561 ttcaaccatc cttcctcgct tgccgaagcc gccaaaaaca gcggtttgaa agtcgaaacc
     7621 caagaaactt ggctgagtag gcaggacgcg caaatgtccg gtatgcccga aaacctgatc
     7681 aatgccgtat tcagcgacga cgtattgaag aaaaaacaca attccgaagt gctgaccatc
     7741 aacagcgaaa ccgcgtgggt cgtccgcgcc aaagaagtcc gcgaagagaa aaccctgccg
     7801 tttgccgaag ccaaagacgc ggtacgtcag gcttatatcc gtaccgaagc cgccaaactt
     7861 gccgaaaaca aggcaaaaga cgtgcttacc caactgaacg gcggcaaggc tgttgacgtg
     7921 aaatggtcgg aagtgtccgt tttgggcgca cagcaggcaa ggcagtccat gccgcccgag
     7981 gcttatgcgg aactgctgaa agcaaaaccg gcaaacggca aacccgccta cgtcaggctg
     8041 atcggtctgc cggcacccgt gattgtcgaa gtacaggctg taaccccgcc ggatgatatc
     8101 gccgcacagc ttccgcttgc aaaacaggct ttggcgcaac agcagtctgc caatactttc
     8161 gacttgttga tacgttattt caacggcaaa atcaaacaga ccaaaggagc gcaatcggtc
     8221 gacaacggcg acggtcagta attgacactt ttgttgacaa aataacggtc ggaatattcc
     8281 ggccgttttc ccatacggcg gaatatgatg agccacaaaa aacaagatgc cttccaagga
     8341 ttgatcgacg cgctgaaggt tttacccaac gtcgggccga aatcggcaca gcggatagcg
     8401 tatcatttgc tccaacacaa gcgcaaagag gctgaaaaac tggtggatgc cttgcagacg
     8461 gcattgaagc aggtttacca ttgcgcgatg tgcaacacgt tttgcgaagg cggattgtgc
     8521 gatatttgtg ccgatgaaac acgcgacggg cggcggctga tggtggtgca tatgcctgcc
     8581 gacgtgtcga atatggaagc ggcaaactgc cacgacgggc tgtatttcgt cctgatgggg
     8641 caaatcaata cggcattggg aatggacgta tccgccatcg cattggacag gctggcgcaa
     8701 cggctgggcg gcggggaagt cgaagaaatc attattgcaa ccgcttttac cgcagaaggc
     8761 aatgcgacgg cgtatgtcct gtccgagttt tttaaaaacc tgccttacaa agtcagcagg
     8821 ctgtcgcagg gcattccctt gggcggcgaa ttggaatatg tcgatgcggg aacgctggcg
     8881 caggcggtgt acgaacgccg cctgattaaa gaaggcgggg cataacgccg ccaatgcaaa
     8941 atgccgtctg aagccttcag acggcatttt tccgggcggt ttacttgcag tttagccctt
     9001 gaatctgcaa tgcgcctccg ttgccgactt tgaacacgca ctgcgtcgcg ctgctgccgg
     9061 acacggtaat gctgccgctg ccgccgttca ggctgatagg ttccttaaca cccataccga
     9121 ctgccgttgc cgccagcttt tgcgcgtcgg cttcggaata aggcgtgttg ttggcatcga
     9181 ttttgatttc tttggcgtag gcagccgcag cggcggataa ggcgatggcg cacaaagcaa
     9241 aagcagttcg gatattcatt gttttcctca agaaacggtt tttctgaagg aaaggatagg
     9301 gcagtttgac gcgttgtgca agttttccgc ccgctctgcc gccccgtccc ccgcactcgg
     9361 gcgttcggac gctgcctggg ttttacggta caataaactt ttgccgccgc ccccaaaccg
     9421 ccgtccgggg cggcacgttt gcagacattt ttaaggtagc gttatgtttt ctctagaggc
     9481 ttggataggc ttgaggtatc tcagggcgaa aaagcgcaac ggctttatgt cgtttatcac
     9541 gatggtttcg attgccggaa tcgccttggg cgtaaccgcg ctgattgtcg tcttgtcggt
     9601 tatgaacggc tttcagaaag aaatacgcgg gcagctcctg aatgtcgcgc cgcacgccga
     9661 aatcggctat atcgataata cggatacgga ttggcgcaac ctgcttcggt ttaccgaaaa
     9721 ccgcaaaggt attttggctg ccgcgcccta tgtttccaat caggcattgc tggccaatgc
     9781 gggcgaaatc aggggcgtgc agatgcgcgg cattttgccg tctgaagaac gcaaagtggt
     9841 ggaatacggc gacaaaatgc cggcaggcaa atttgaagat ctgattccgg gcgagtttga
     9901 cattatcctc ggtgtcggct tggcggaggc tttgggggcg gaagtcggca ataaagttac
     9961 cgtcatcacg ccggagggca atgttacgcc cgccggagtc gtaccgaggt tgaaacagtt
    10021 taccgtggtc ggtctggtta agacgggtgt ttacgaagtg gacaactcct tggcgatgac
    10081 gcatatccaa gacgcgcgcg tactgtaccg cttggataag gaagttgcgg ggctgcggct
    10141 gaaactcgcc gacccgcaaa acgcccccgc cttgacggca acactgattc cggaggcgca
    10201 aagggatacg gtttgggtgc gcgattggac gttcagcaat cgaagctatt ttgaggcggt
    10261 cgagttggaa aaacggatga tgttcatcat cctgacgctg attatcgccg tggcggcgtt
    10321 caaccttgtc tcttccttgg tgatggcggt tacggaaaag caggcggaca ttgcgatttt
    10381 acggactttg ggtctttccc ctggcggcgt gatgaagatt tttatggtgc agggcgcgtt
    10441 ttcaggcttt ttcggcacgc tggcgggtgt ggtctgcggc gtgcttttgg gttggaacgt
    10501 cggcagggtc gtggcgtttt ttgaaaacct gctcggtgtc cacctcatca attcgcaggt
    10561 ttattttatc gactacctgc ccagcgatgt cgatatgggc gacgttgccc tgattgcctg
    10621 catttctttg ggactgtctt tcgttgccac gctctacccg agccggcgcg cgtcaaaaac
    10681 ccaaccggcg gaggctttgc gttatgagtg agttgatttt gaaatgcgaa ggcgtgggca
    10741 aacgctatcg ggacggcggt ttggacgttc gggtgctgca cggcttggat ttggaaatcc
    10801 acgcagggga aagcaccggc atcatcggtt cttcgggcag cggcaaatcg acgctgctgc
    10861 atattttggg cgggctggat atgccgtctg aaggcagggt gctgctgatg ggcgaggatt
    10921 tgcgtacctt aaaccagcgg cgtttgggcg atttacgtaa ccgccatctc ggtttcgtgt
    10981 accagttcca ccatcttctg cctgaatttt cggcactgga aaatgtgatg atgccgcttc
    11041 tgatcggcaa aaaaagccgt gaagaggctg cggaggcggc aatggcgatg ctggaaaagg
    11101 tcggactgaa acaccgttcg acgcaccgcg cgggcgaact ttcaggcggt gagcggcagc
    11161 gtgccgccat tgcacgcgcc ttggttaccc aacccaaatg cctgcttgcc gacgaaccga
    11221 ccggcaatct cgatcgtgcg aacgccagga atgttttgga tatgatgctg gaactgaaaa
    11281 cagaattggg aacggggctg gtggtcgtaa cgcacgacga cgaactcgcc ggccgcttcg
    11341 agcgcgtgat ggtcatgaaa gacggcagcc tgcaccccaa acagggcgca aacgcctaaa
    11401 cagccgaaac aacaccgcgc cgtctgaagc cccacgcctt cagacggcat ttggataagg
    11461 aataaaatgg aacggcagac agatgaattt gcccaagcgg cggcaagggc ggcaatccgt
    11521 tttttggaac actatgcggg ttccggcgac gaagtgcttg caaactgcac cgaacgcctg
    11581 tttcaggcat tgcaaaacgg tcattcgttt atccgtttga gcggtggcga ggctgacgcg
    11641 ctgtcggcac tcgcacccgt tgtcggaaca tccgccgcgc ctttgatttt ggaaggcaga
    11701 aggctgtttt tgggcaggat gtggcagttg gaatacgatt tggctgccga gataaaacgc
    11761 ttggcggcag ccggcacatc cgcccccgac gcggcaggcg cgaggcaaaa cctcgcaaaa
    11821 tggtttcaag gcacgggcag cgaagggcag cgcgatgccg ccgccttggc actgttgcag
    11881 tcttttatgg tgattaccgg cgggccggga acgggcaaaa cgacaacggt tgccaaactg
    11941 ctggcgctga tttgcggtga agacgaaaat cttcccaaca tcgcgcttgc cgcaccgacg
    12001 ggcaaagcgg cggcacatat ggcgcgcgcg ctgcaccgtg caatcaacgg ttttgacgcg
    12061 ccggaggccg tccgccgcca tttgctcaaa ctggaagggc aaaccgtcca ccgactgctg
    12121 aagctgcgcc cgcccaaaat gcaggcggcg ttcaaccctg tttacccgct gccgtttgac
    12181 gtattggtta tcgatgaagc ctctatgctg gatacggcat tgatgctgca acttttaaaa
    12241 gcggtcaaaa ccggcgcgcg cgtgattctg ctgggggatg aaaaccagct cccgtccgtc
    12301 ggaatagggg cggtgctgtc cgttttgtca caaaaaaccg ttttggacgg agaaacgcac
    12361 caaaggctgg ccggcttcct tccggaacac ggtttcagcg tcagcgcaaa tccacccgtg
    12421 ttggcgcaaa acaccgccca tctgtcgttc agccaccgct tcggcgacaa cagcggcatc
    12481 ggctgccttg cccgtgccgc cgtatcgggc gatgaagggg cgtgggcatt gtttgaccgg
    12541 tttccggacg aactggaaca ttcggaatgc agtccgaacg ctcgagtcga aaggttgtac
    12601 cgggcacaca aagcctattg gcaggcggta aaagacggca atatcgaagc cgcatacgcg
    12661 ggcatttcgg atatcgtggt tctggcagct tggcggcagg atgcggaaga cttcaacgaa
    12721 gcctattgcc gccatgtacg ccgcaaaatg aacataccgg aacatttggc atattttgcc
    12781 ggagagccga ttatgatcag gcagaacgac tacgcgcttg aactgttcaa cggcgacatc
    12841 ggactgatta tggaagatgt cggacggcag ggcagccttg ccgcctattt tgccgatgcg
    12901 gacggattta aaaaggtagc ggtaagctgc ctgcccgaat ttgaacccgc attcgccatg
    12961 accgtccaca aaagccaagg ttcggaatac cgggaagtat ggctgctgcc gccttccgcc
    13021 gcaccttcgg acgaagggga cgatgcattg tccggattga gtaaggagct gttatatacc
    13081 gccattaccc gcgcgcgaga gaagttcgta ttcttcggcg gggaagaagc cttccggcaa
    13141 gctgccgcca ccgtcaaaac gcgtcagacg gcattgggca gtatgctcga gcgggtattt
    13201 tcacaagaat aatccgcccg aatgccgcgc cgccgcccct tatgcctttt tcaaacggta
    13261 taggaaagtg gtttcccggg ttcgcgcaaa agcaagcgga tcgctcggat tcgcggcttt
    13321 tttgtgcttc ggcttggttt tcatcatatc ggcaacacgc aaacccgcct gagcaaatgc
    13381 cttatccaga aaatcggacg gacgcaggtg cagatgttcg gcaagatcgg aaatgatcag
    13441 gcggatttct ccgtcgggat tcagatgttt cggcgcatcc cgcaaaaacg cagccagcat
    13501 cgcagattcg gggtcgtata acgcggattc gacggcggaa gtcggcttgg cgggaagcca
    13561 gggtggattg cagacaatca gatcggcaaa cccttcggga aacagatcgg tttcccgtat
    13621 ctcaacctgt ttttcaaagc ccaaacgggc aatattggca cgggcgcagg cgacggcttt
    13681 cggattggta tccgtgccga tgacggaagg aatgccctgt ttcgccaaaa tggcggcaag
    13741 cacgcccgag cccgtcccga tatcgaatgc cgtctgaaaa cccgttgacg gcgcatgggc
    13801 gagcaggtcg aggtattcgc cgcgcaacgg cgagaatacg ccgaaaggaa cgtgtatgct
    13861 gccgcccagc tgcggaa
//
LOCUS       DAWHRS010000042        13838 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWHRS010000042 DAWHRS010000000
VERSION     DAWHRS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.042"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(178..825)
                     /locus_tag="WIB82_000871"
     CDS             complement(178..825)
                     /locus_tag="WIB82_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ3842191.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(822..1190)
                     /locus_tag="WIB82_000872"
     CDS             complement(822..1190)
                     /locus_tag="WIB82_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ3842192.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(1200..1864)
                     /locus_tag="WIB82_000873"
                     /pseudo
     CDS             complement(1200..1864)
                     /locus_tag="WIB82_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2029..2748)
                     /locus_tag="WIB82_000874"
     CDS             complement(2029..2748)
                     /locus_tag="WIB82_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ3842193.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2832..4145)
                     /locus_tag="WIB82_000875"
     CDS             complement(2832..4145)
                     /locus_tag="WIB82_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ3842194.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLTRHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTIPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFAAAETCLQSLSDAV"
     gene            complement(4543..5415)
                     /gene="hpnD"
                     /locus_tag="WIB82_000876"
     CDS             complement(4543..5415)
                     /gene="hpnD"
                     /locus_tag="WIB82_000876"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ3842195.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTILYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDGKTLQYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5586..7448
                     /gene="hscA"
                     /locus_tag="WIB82_000877"
     CDS             5586..7448
                     /gene="hscA"
                     /locus_tag="WIB82_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ3842196.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKIALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7525..8181
                     /locus_tag="WIB82_000878"
     CDS             7525..8181
                     /locus_tag="WIB82_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ3842197.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISEVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPRKSK
                     IRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            8217..8978
                     /locus_tag="WIB82_000879"
     CDS             8217..8978
                     /locus_tag="WIB82_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ3842198.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNIEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            9033..9374
                     /gene="fdx"
                     /locus_tag="WIB82_000880"
     CDS             9033..9374
                     /gene="fdx"
                     /locus_tag="WIB82_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ3842199.1"
                     /translation="MPKITVLPHTTLCPEGAVIDDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9797..10351)
                     /locus_tag="WIB82_000881"
     CDS             complement(9797..10351)
                     /locus_tag="WIB82_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ3842200.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRDLIALDKAAAGCDSAMLRAQRWLIYTQIAERLAGSGGGFT
                     VTVESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEARVERYPAYCPTVALKV"
     gene            10564..10761
                     /gene="iscX"
                     /locus_tag="WIB82_000882"
     CDS             10564..10761
                     /gene="iscX"
                     /locus_tag="WIB82_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ3842201.1"
                     /translation="MKWTDTQRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10829..11230)
                     /locus_tag="WIB82_000883"
     CDS             complement(10829..11230)
                     /locus_tag="WIB82_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ3842202.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            11442..12401
                     /locus_tag="WIB82_000884"
     CDS             11442..12401
                     /locus_tag="WIB82_000884"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ3842203.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            12469..13779
                     /gene="tilS"
                     /locus_tag="WIB82_000885"
     CDS             12469..13779
                     /gene="tilS"
                     /locus_tag="WIB82_000885"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ3842204.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAASDTLAMGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
BASE COUNT         3152 a         3630 c         3879 g         3177 t
ORIGIN      
        1 tacaaccacg ccggaattaa gtttaaattt gaataaaagg ttcgggttct gcaaaataca
       61 gaacccgaac cttgttcgga tattgaaacc ggctgcccga ttttgggcgg tgcggcttgc
      121 aagtatcaag attcgcatat gccgtctgaa gctcggagag gttcagacgg catatgctta
      181 tttgggctgc tcttcaacga atctcggacc tttcaagatg ccgttgtgag aatagggcga
      241 cagcaggttg tatgcggcgg ttttggaaac ctgataaccg cggtcggtca ggctgttggc
      301 aatctgattg accactgcgc tgaccaaagc ccccaacagg ccgctgttgc tgttgttgct
      361 gccttcgcgg atgctggccg aacccgacca caactctttt ccgttgcggg aatcgaccag
      421 ccgtgctttg gcggatacgg tcgtcacgct gtctaaaatt tgatatgaag tgccgtattc
      481 ggtaaccgta atgtacaaaa ccgcatcatt gccgaaaatc tgatgcagtt tttccggccg
      541 gacggcgtga atatcggcgg cattggtcaa gccgttttgt ttgaaggttt cctccacgac
      601 tgcggcgggg aagacgtaat agccggcttc ggaaagcggc gcggcggtcg aagccagtac
      661 accccatgtt ccgttgacat cgggcgattc gttcagcggc ggaaccacca aaattgaagc
      721 cggtttgctt tccttgaatg acgtgtagtc gaaatcgggc gctttttgaa cttggcaggc
      781 agacagcgcc aacacggcgg caagccctaa aatcaaaggt ttcatcgctt gcctccttta
      841 cctgttttca tcaggaagtc cataaatacg cccgattcgg gaaacagcct tttctcttct
      901 tcaaactggc ggaacgcgcc ctctttgtct cccgaacggg aaagcagcag tcccagatgg
      961 gcgtgcgcac ccggggcggc attcattttt ttgttgccgg cttccacaaa gtatttttcc
     1021 atcttttcgg tctgcttgcc caacgaagtg tcgtcgtttt tcaaaccttc atagacggta
     1081 tcgggatagc cgccgtaata atacagggat ttttgcccgt tgccgccgca ggcggtcaga
     1141 gccaagaccg ccgcacacag cgacaaacgg ctcaaggttt tcggattcat catttctcct
     1201 taacggttgg gttgccatgc gccgttgtca acagcctgaa ccaggctgtt gacggcttcg
     1261 cggattgcca agtctaaaac tttgccgttc aaagtcgcat cgtagccgga agtgccgccg
     1321 aaaccgatga tttcacggtt ggaaagtgcg tattcgcccg cgccctgtgc ggaatagatg
     1381 atttcggaag tattgacgtt gacgatattc agagccactt ttgcataggc gatttgccgc
     1441 gacccaaaat gccgaagagc tgatgatcgc cgacatctct gcgtccgaat tcggttacat
     1501 cgccggtaac gacataatct gcgcctttca ggttatgcgc tttgccggaa atgccggatt
     1561 cctgttttaa tgcgttcaaa ttggtgcggt tcagtacgtt gaagcggttg gtctgttgca
     1621 ggtgcgttac cagaatggtt tttgcctggc tgcccaaacg gtcttccccg tcggagaaaa
     1681 tgcctttttg gaagctggag cggttgtcga atgttccgac ggaaatcggg gtacgaacac
     1741 cgtgatattg cgtattgtag gaggcgactt tctctacctc gagactgcgt gaggattcgg
     1801 tcgcacagcc ggtcagtgaa acggcagcgg cggcaaggac aacggcggtg gaaacggttt
     1861 tcataaaatt taccctaagg tcaagttaag gaaataacgg gttatcatta ttgtccttat
     1921 gtaaatttaa gtcaaggtgt ttgtctgtgc gggacggatg cgcgcggaag gtacggatat
     1981 ttttcaaacg gaatcgcggc gcgggcggga tgcctgcctt ttccggattt agaggtaaac
     2041 gatttcgcca ctccgccctt tgctttcggc acttgcccac cagacaaatg cgggcagcac
     2101 gtccccgtag cttttgcgtt cgcttttggc ttcgcccgga tgggatttga tgcgttgcgg
     2161 ggaattgatg gggccgggga cgaggacgtt ggcgcgcagg ttgccgaagc gttcccattc
     2221 gtcggcggcg actttgcaca ggtagttcaa cgcggctttg gacgcgccga agccgcccca
     2281 gtaggctttg ggtgtttcgc cgtggctttc gccgacgaag atgacggacg cgtcgggcga
     2341 ctgcttcagc agcgggaaca gggcgcgggt cagccccata ggtgcgacgg tgttgatgcg
     2401 gtattggttg acccattcgg cgacggtttg gaaatccagc ggcgagaggg cgtaaaaata
     2461 gccggcgcag tggacgatgc cgtccagttt gccttgcgtg gcttcggcaa tggtggcggc
     2521 gaactgattg aattcttttt cttccgcact catcaggtcg aaacggatgg cgaagggttc
     2581 ggggtgtccg gcttcgacaa tcgcgtcata gactttttcc agttttttct gatggcgtgc
     2641 caccagaatc acggttgcgc ctgccgccgc ataggctttg gcgacctgtt cgcccagacc
     2701 ttgcgatgcg ccggtaatta agatggtttt gtcggacagt gtcgccatac tttttccttt
     2761 ttggtggtcg gtcgggggta ttttagcgtt ttgccgcacc ttgtaaagcg tcccgatgcc
     2821 ggccggcgtt ttcagacggc atcgctcagg ctttgcaggc aggtttccgc cgccgcgaaa
     2881 cctgattgta cggcggcttc gagcgtggcg gggtagtccg ggtggaggta gtcgccggcg
     2941 gggaagatgc ggtgccggtg cagccacgac aaatccggcg gcggggcatc ggctgcggtt
     3001 gtggcgcgtt tttcggtgat gacgcgcacg gcttcgggtt cgcccaaatg cggaaggatg
     3061 cgtttgaggt cggcgtgaac tttgtccgcc cacgctcggt ttgtaaacgc gccgacgcgg
     3121 tcggaaacgc tgatgacggc ggacacttcg ttttcaggca gtccgagcct gccccggcaa
     3181 agcagccatt gtgccgtgcc gtcggcaagg ccggtcagcg gggcgggcag gcggacgggt
     3241 tcggcgtagc gcagatagac ggtggtgatg gcgtgatagc gaaggttttg atatgccgtc
     3301 tgaacgtgtt cgggcgtgcc ttcgggcagg agcgcggcgg cgtggtaggg cgcggtggcg
     3361 gggacggcgg catcgaaagc ttcgccgttg acgagcactt tcccgtccgg gatggtgttc
     3421 agacggcata cgcgcgtttc gaggcggatg tccgcgccga gccgttgaag ctccgccaag
     3481 gcgggttcgg cgacgattgc gcccaaatcc tgcttgggta ggagatagtc gctgccggat
     3541 tttttcgtca gcacgccgtc ggacaaaacg ttgcacaaca cgcgcaggct tgcggtttcc
     3601 aaaggcgtgt tgagcgcgcc ccacaccagc ggctgccaaa actgcatcac ggcggcacgc
     3661 ggcacgttcc gctgtttcag ccattgtgca actgtcgtgt cgggctgtcc gaggcgtgcg
     3721 gacttctgca aatcggacat atcggcaagc agtttggctt tgaatgcgga cggtacgcgc
     3781 cgggcaagca gcacgccgcc caaaatatgc agcggtgcgg gcagggggag ggcgcggaac
     3841 tgcaaaccgc cgtgcatatg ccagtgcagc ggtacgcgca aaaaggcggc acggggatcc
     3901 gaaccgatgg ttttcatcag gcgcaacacg ccccggtatg cgccgagcaa aatgtgctgc
     3961 ccgttgtcca aaaaaccgaa accgtcggta tttccggcca gtgtgcgcgc cctgccgccc
     4021 gcctgccggc cggcttcaaa cagggtaacg tcggcgtgcc gcgtcaaggt gacggcggcg
     4081 gacaagccgg cccagcctgc gccgatgacg gcgattttcg ggcgcggatg cggcgtgttc
     4141 atcatttatt cctccaatgg ttttgcagcc gtatctattt ccgtttccga aaatgacggt
     4201 agaaaaggat acaggctaaa aataaaggca gcagaaagcg cgcataggga taccacggat
     4261 ggtctgcatg ccagtatccg taccgtccct gtggaacggt atcatagcgg taattgtcgg
     4321 ggaaaaagtt gatccaaacc gttactgcca gccataaggc tatatccctg actgctttca
     4381 tttacttctg ctcctgttta aattcccagc aattcgattt caaagcgcga acgccaacgg
     4441 gattgcgcgg ttacgatgca gactttcaga cgatggttga aaccccgtcc ggcggggctg
     4501 tgggaatcgg gtttgcctga cgggcggcgg gcggtatgcg ccttatgccc gttccggcgt
     4561 gccggggcgc ggtttgaatc cgaataacca ggttttcagg gcaatgcgtt ttttgcgcgg
     4621 cgaagggagg gcgattttgt atttgaggac gttttgcgcg ccgtcccggt cgatttcgtt
     4681 caatagctcg taataaaccg ccgccataac cagtccgact ttttgggctt ttttatcggc
     4741 agccggcagc agcgatacgg cttcgcggta ggtttcacgg gcgcgtttga tttggaacgc
     4801 catcaattcg gcaaaattgc ccgtcgggct gcattgcaaa atcacgcttg cggggacatc
     4861 aaaccgctgc atttcctcca tcggcaggta aatccgtccc ctgcgcgcgt cttcgccgac
     4921 atcgcggatg atgttggtca gttgcagcgc aagtcccatc ttgtcggcgt attgcagcgt
     4981 tttgccgtct gaaaacccca aaatccgcgc aatcaggcag ccgactacgc ctgcgacgcg
     5041 gtggcaatac agtttcaatt cctcaaaact gccgtaacgc gcctgaacca aatccatctg
     5101 catcccgtcg attaaggctt ccagttcata tttcggcagc ttgaaggttt ccttaacctg
     5161 ccgcaaggcc tgattgacgg ggtgttccgg catcgcgccg ccgaatacct tgtccaaatc
     5221 gccgcgccac cagttcaatg ttgcctgtgc aacatcgggg ttggaacatt cgtcaaccac
     5281 atcgtccaat tcgcggcaaa aggcatataa aatcgttacc gcatcccgtt tttcctgagt
     5341 caggaaacgg aagcccgata aaaaactgga gcggctttct tctgcttttt gacggcaata
     5401 gtcgagtcct ttcacgattt atattcctaa tgatgggcgg gaaaggcgga ttttatcggc
     5461 atttggcggt agagggcaat ttcggcggca cgacccaatc cttagcggtt tgctcaacta
     5521 tcggcgcaaa ttctgttaaa atgccgcttt ccttccttta cacaccgcac cgacaggcag
     5581 aatttatggc tcttttgcag atttcagaac cgggtatgtc cgccgccccg caccggcacc
     5641 gtttggcggc aggcatcgat ttgggtacga ccaacagctt ggtcgccacc gtccgcagcg
     5701 gcagtgccgc ctgcctgccc gatgccgaag ggcgcgttac cctgccttcc gtcgtccgct
     5761 atctggaaaa cggcggcatt gaagtcggca aaatcgccct gtccgcccaa aaaaccgacc
     5821 cgctgaacac cgtcagctcc gccaaacgcc ttatcggacg gacgcttgcc gatctgcatc
     5881 aaaatacgca ttacctgcct taccgtttcg gcgacaatca acgcgttatc gaactgcata
     5941 cgcggcaggg ggtgaaaacg cctgtcgaag tgtcggcgga aatcctcaaa acccttaaat
     6001 cgcgcgccga agaaaccttg ggcggcgatt tggtcggcgt ggtgattacc gtccccgcct
     6061 atttcgacga tgcccaacgc caggccacca aagatgccgc gcgtctggcg ggtttgaacg
     6121 tattgcgcct gctcaacgaa cccaccgccg ccgcaatcgc ctacgggctg gacaacgcct
     6181 cggaaggcac gtttgtcgtg tacgacttag ggggcggcac attcgacgta tccgtattgc
     6241 aactgaccaa aggactgttt gaagtcaaag ccaccggcgg caacagcgcg ttgggcggcg
     6301 atgacttcga ccatcgtttg ttctgctacc tgctcgaaca aaacggactc tcccaactca
     6361 acgaacaaga cagccaactc ctgctttcgc tcgtccgcgc cgccaaagaa caattaacca
     6421 cgcaaaccga agcgcgcatt caggcgacgc tttcagacgg catggcaatc gacacaagca
     6481 tcagtcgcgc cgagttccac aacctgacgc agcatttggt gatgaaaacg ctcgaaccgg
     6541 tcaaacaggc gttgaaagat gccggtgtcg gtaaaaacga agtcaaaggc gtaattatgg
     6601 tcggcggttc gacccgtatg ctgcacgtcc agcaggcagt cgccaccttt ttcgggcaaa
     6661 ccccgctgaa caacctcaac cccgacgaag tcgtcgcgct cggcgcagcc atacaggcga
     6721 acgtcctcgc aggcaacaaa gccgacggcg aatggctgct gctggacgtt acgcccttgt
     6781 cgctcggttt ggaaacctat ggcggcttgg cggaaaaaat catcccgcgc aattccacca
     6841 tccccaccgc gcgcgcgcag gactttacca ccttcaaaga cggtcagacc gcgatgacga
     6901 tacacgtcgt acaaggcgaa cgcgaactgg ttgccgactg ccgcagcctt gccaaattca
     6961 ccctgcgcgg cattccgccg atggcggcgg gtgcggcgcg tatccgcgta accttccaaa
     7021 tcgatgcgga cgggctgctg tccgtttccg cccaagaaca aagtaccggc gtacacgcgc
     7081 aaatcgaagt caaaccttcc tacggtttgg acgacggcgc gattacccga atgcttaaag
     7141 acagtatgga caacgccgcc gaagatatgg cggcacgcgc ccgcgccgaa gccgtggtcg
     7201 aagccgaaag cctgaccgat gccgtcaacg ccgccctcga gttggacagc gatttgctgg
     7261 atgccgaaga attgcaacag attcggcaag gtatcgccga tttgcaaggc cgtctgaaag
     7321 acggaaaagc cgaagacatc cgcgccgccg ccgccaaact cggcagcatc accgacaatt
     7381 tcgccgccaa acgcatgaac cgcaacatcc aacgtgcgct gacaggccag agtgtcgata
     7441 atatttgata cttaaacggt ttcagacggc atagagataa cctgatgccg tctgaaggct
     7501 cgaaaacact tgaaaaacat cgatatggaa aagtcaggca ttgtctatct gatgaaaacc
     7561 gtcatcaagg gcgtatataa aatcggcatt tcggaggtaa gcaattttga aggcagaatg
     7621 cgccatttgg aaaacaacgg ctatgcgaac gttgccgggt tggaacgcat cctcgctgtc
     7681 aaaaccgaca attacaaaga aaaagaaaac ctgctccatg aaattttcag caaaagcagg
     7741 ataggcgata ccgaattgtt cgccgtggac gaaaaccttg tgaaacgttt gtttttatcg
     7801 cttcgcggcg aaatcgtgtt cccgaaaaac gaaacggcgg aatcggaatt tgaaaaaagc
     7861 gtccacgaac gcaggcagga agggaatgcc gggtcaggcc gcaaacaact gcttgatttg
     7921 gtacggcgcg gacaccggga atacccttac gcgctgcccc ggcttttggc gggcgcggca
     7981 tcctacaagc cgagaaaatc gaaaatccgc ctttttaaag aagcatattt cggcaaaagc
     8041 ggcacgaggc tgaccgacga aattgcagac ggcatccata tttacacctg tttttcgcgg
     8101 gcggatttgg aaaaagccta ttccgaatat ttggaacttt tcaaatccga atcggatgcc
     8161 gaaggcagaa aaccacggta aggtgcaaac agataccgta cacgttgagg agcagtatga
     8221 tgggcgattc cgtcatttat tatgtagaac aggcagacga accggtaaac cgtgccggcg
     8281 aacgcgcccg taaaacattc aaatattttt ggcgcgagct tttttgggaa cgccgccgca
     8341 tcattcccgc cttagatttt gccatggtca aagccccttt tttccaagac ggcgaagacg
     8401 gcgaaatttg cgaacatatg tggatcgacg atatttattt tgacggtctt tatatttacg
     8461 gtgtgctgaa caataaaccc ggcgaactga ccaatatcga acaaggcgaa agcatttgcg
     8521 ttccggttga cgacatcagc gactggatgt tcgtgtgcaa cggcattccc tacggcggct
     8581 ttaccataca ggcaatgcgt ggacagatga cggaagagga gcgtaccgaa cacgatgccg
     8641 catggggaat cgatttcggc gatcccgggc aggtattgct ggtgtatgaa gaaaaagaac
     8701 atcccgaaaa tctggaagag catccgatgt gccggaactg tattgacgat tttcggcaac
     8761 agttgtccca aaatccggat tttctgcatg agcaggacga agacggctat acgccgcttc
     8821 atcatgaagc catcgcagga aatgcactta tggttcaagc catgcttgaa tacggcgcaa
     8881 atcctgcctc aaagacatcg gaaggctata ccgccctcga ttttgcccgc ctgacgggct
     8941 ggcaaaatgt tgccgacctg ctcgaaccgc gacattaggc agacagtttt ccgaaaacga
     9001 acacaaacac tttttacaga aagacaataa aaatgcccaa aatcaccgta cttccacaca
     9061 cgacattatg ccccgaaggc gcggttatcg acgacgcgcc ggaaggcaaa accgtcctcg
     9121 acgtactgct cgaccacgac atcgaagtcg atcacgcctg cgaaaaatcc tgcgcctgca
     9181 ccacctgcca cgtcatcatc cgcaaaggtt tcgacagttt ggaagaaccg accgaattgg
     9241 aagaagacct gctcgaccaa gcatggggtt tggaagccga ttcgcgcctg agttgtcagg
     9301 cggttgtcgc cggcgaggat ttgattgtgg aaatccccaa atacaccatc aaccacgcgc
     9361 gagaagaaca ctgaaaacag gccgtctgaa gccggcgcgc ttcagacggc attgttgcgc
     9421 ggataaggcg caatcgcccg aaaacaggcg ttcgtacagg cggaaatttc gattctatag
     9481 tgaattaaca aaaatcagga caaggcggcg agccgcagac agtacagata gtacggcaag
     9541 gcgaggtaac gctgtaccgg tttaaattta attcactata tattgatttt tatcggtttt
     9601 ctgacggaat aatccagtgc ggcatccgag gcggattact cggacgcgat gcaccggtat
     9661 ttatcggttt tgcagccgga aaaaccgccg gcgggttata gtggattaaa tttaaaccag
     9721 tacagcgttg cctcgccttg ccgtactatt tgtactgtct gcggctcgcc gccttgtcct
     9781 gatttttgtt aatccactat acttttaggg cgacggtcgg gcagtatgcc ggatagcgtt
     9841 ccactctcgc ttctatattg attttgattg gttttctgac ggaatgaccc gatgcggcat
     9901 ccgggacggc ttgtgttttt tcctgcccgc ctgccggatt ttcccatcct tgcgtgaaac
     9961 cgaaagagac ggcggcgcgg cggacaagct cgagatagcg tccttcaagc tccggacagg
    10021 cggcggacac gctttctacc gtaaccgtga aaccgccgcc cgacccggca aggcgttccg
    10081 caatttgcgt gtagatgagc caacgttggg cgcgcaacat tgccgaatcg caaccggcag
    10141 ccgctttatc caaggcaatc aggtcgcgcc ggttttggtg gtgaaggcaa accgttactc
    10201 tggagaggat atgttgcccg tccaatattt gatacagttt gcgtatcagc agaatcaggc
    10261 ggtcaaactc ctccatctcc gacagggaat caggatggtc ggaagcggta taaaccagtt
    10321 tggacaattt tgcggcaaac atattgttca tcaatcttcc ttgtcggttg aaaccccgct
    10381 cttcggggcg gtagaatcag atttgtttgg gagggacaac tcctcccaga tcaggacgac
    10441 acataggctg gtgcttgatg tgttgtccgg cgagttgaaa cattcagcaa tcctcaaggg
    10501 gcggcagttt tgccgaaaca tattctacac ggcttcaatg ccggacgata aaaggaaatt
    10561 catatgaaat ggaccgacac ccagcgcatc gccgaagaac tctatgacct gcacggcgaa
    10621 accatcgatc ccagaaccgt gcgctttacc caactgcgcg acctgattat ggcattgccc
    10681 gaatttgacg acgaccccgc ccgttgcggc gaacgcatcc tcgaagccgt gcagcaggca
    10741 tggatagacg aggcggaata agtttcggga atgccgtctg aaatgcggcg gtacgcggtt
    10801 cgtgcttctg tttgcagcgg gaatggtttt accagtctcc ttttttcagc ctgtccagtt
    10861 ggcggcggtc gcgcttggtc ggtctgccgt cgggataggc ggaagtgatg cggctgaatt
    10921 ggtcgagctg tttgcgctct tccctcaatg ttgccgtttt cgcgtcctct tcatacagaa
    10981 gccgcgcctc ggatgccggg cggcgttggt ggttcaaacc tttaaccttg attttatagg
    11041 gaagggaatt gagcgtcagg tcgataatat cgccgatgtc tatggtttta ctgtttttga
    11101 ccttcgagcc gtttacttga accctaccca gttcgatgtg cttttgcgca agggaacggg
    11161 tcttgaaaaa acgtgccgcc caaagccatt tgtccagccg catggcggaa gaatcgtgct
    11221 tgtctttcat acgattttgt ttgaaataat tgaatttgtt tcgagtttag cataagatac
    11281 gccgccttat aactagtata tatgcactaa tccactgttt tccatgctgt ccgaacacaa
    11341 aaagagggta tggaaaagcc gttttggaca ataaattaac tgcggaatat gcacaaatag
    11401 cgtatgatag cggcagaatc tgttgatgag agcttcattc tatgaaacct gtttttttgg
    11461 attttgaaca acccatagcc gaactgacca acaaaatcga tgagctgcgt ttcgtccaag
    11521 acgagtctgc cgtcgatatt tcggacgaaa tacaccgttt gcagaaaaaa agcaacgacc
    11581 tgaccaaatc gattttcagc aaactcacac ccgcccaaat ttcacaggtt tcccggcatc
    11641 cgcagcgtcc ctatactttg gattacattg aggcactgtt taccgatttt gaagaactgc
    11701 acggcgaccg ccactttgcc gacgattatg cgattgtcgg cggattggcg cgtttcaacg
    11761 gacaaagcgt gatggtcgtc gggcatcaga aagggcgcga caccaaagaa aaaatccgcc
    11821 gcaacttcgg tatgccccgt cctgaaggct accgcaaagc cctgcgcctg atgaagacgg
    11881 cagaaaaatt cggcttgccc gtaatgacct ttatcgatac gccgggcgcg tatcccggca
    11941 tcggcgcgga agaacgcggg cagtcggaag ccatcggcaa aaacctgtac gaactgacgc
    12001 gcctgcgcgt tcctgttttg tgtaccgtca tcggcgaagg cggttcaggc ggtgcgttgg
    12061 cggtcgccgt aggcgattac gtcaatatgc tgcaatactc gacctattct gttatctccc
    12121 ccgaaggctg cgcgtctatt ttgtggaaaa ccgccgaaaa ggcggcggat gcggctcagg
    12181 ctttgggcat tactgctgac cgcctgcaaa agctggactt ggtcgatacc gtcatcaaag
    12241 aaccattggg cggcgcgcat cgggatttcg ggcaaaccat gaaaaacgta aaagccgttt
    12301 tggaaaaaca actgcacgaa gcgcaaagca tcccgcttgc cgatttgctt tcgcgccgtt
    12361 tcgaccgcat tatggcttac ggcaaatttt cggaacaata attcaggtag aacaagcagc
    12421 aagcagtttg tctgaaactg cttgcttttt ctttatcggg acggaaccgt gctgacttta
    12481 gatgcgtttg agcaatgctt gaaggattgt tttcctcaag gtctgaatgg aaaaaaaaca
    12541 gcggtggcat taagcggcgg cttggattcc gtcgttttgc tgcatctgct tgtccgcgcc
    12601 ggaaaaaagg gcggttttat tccggatgca ttgcatatcc atcacggctt gagtccccgt
    12661 gccgacgatt gggcagattt ctgccaaaac tattgcgata tgctcggggt ggggctggaa
    12721 acggttaagg tctgcgtgga aaaaaacggt ttgggcatcg aggcggcggc aaggcaaaag
    12781 cgttatgccg cgtttgccga aaaaggcttt gacgttttgg cgttggcgca ccacagggac
    12841 gatcaaatcg aaacctttat gctggcggtc gcgcgcggcg gcggtttgcg cgctttggcg
    12901 gctatgcccg ccgtccgccc ttttggggaa aaaggcatca tctggcggcc cttgctgcct
    12961 ttttcacgcc aagatatatg ggattatgcc caaaaacacg gtttgccgaa tatcgaggat
    13021 gaaagcaata ccgatacggc ttatttgcga aaccgcttcc ggcaccgtat tttgcccgaa
    13081 ctttcggcgc agattcccca tttcgggcgg catgtgctga acaatgtccg cgctttgcag
    13141 gaagatttgg ctttgttgga cgaggtcgtc gttcaggact gccgttgggt ttgcggggcc
    13201 ggttatttcg atacggcgcg gtggctgacg ttttccccgc gccggaaaac ccatattttg
    13261 cggcattttc tgaaggaaaa cggcattccc gtgccgaatc agaatgccct tgccgacatt
    13321 gcccgggttt tgacggaggc aaaaaccgga cgttggaact tgcaaggctt tgaattgcat
    13381 cattatgcag gcaggctgtt tgtgttccga ctggaaaaaa cggataaact gcggtttttg
    13441 aaagacaggc agataagcgg aaatttaagg gaaatattga cggggcaggg atttgtgttg
    13501 aagcggcatc cgtttgggct tcctgagcat cttttggagc aggacggaat tttgaggacg
    13561 gtagcggcat cggatacgtt ggccatgggc ggcatccata aggatgtgaa aaaaatcctt
    13621 caggggaaac gggttttgcc tgtcctgcgc ccaatttggc cgcttgttgc cgacagcgga
    13681 aaccgtccat tggcgttggc aaactgttgt gcggatttcc aatactcggt ttcagacggc
    13741 attttgcccg tccatcctga ctttcccatt ttattttgat aatatcgcaa acagatttcg
    13801 gcggcgttca gtcgggtatt gtccggttgc atatttct
//
LOCUS       DAWHRS010000043        13829 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWHRS010000043 DAWHRS010000000
VERSION     DAWHRS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.043"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(33..923)
                     /locus_tag="WIB82_000886"
     CDS             complement(33..923)
                     /locus_tag="WIB82_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ3842205.1"
                     /translation="MFPVFHLSGESRRQMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDTLPFAVIAGFPEIMLDTADSLAKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKPVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITKSM"
     gene            987..1643
                     /locus_tag="WIB82_000887"
     CDS             987..1643
                     /locus_tag="WIB82_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ3842206.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFVLFLLVFRLCWGIWGSDTARFSRFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLILPMITGFKYIEGKTSIRFAGKAALAAALSVASLAAAAILLLS
                     "
     gene            1656..1814
                     /locus_tag="WIB82_000888"
     CDS             1656..1814
                     /locus_tag="WIB82_000888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842207.1"
                     /translation="MPSEALFALRTASPHHANGLNARNGFRPFFFRNRYKTENRRIRS
                     RPHIAFSF"
     gene            1939..3381
                     /gene="cobA"
                     /locus_tag="WIB82_000889"
     CDS             1939..3381
                     /gene="cobA"
                     /locus_tag="WIB82_000889"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ3842208.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLNAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPHSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENGEGENRVGQAYPALGGLNAGQRAA"
     gene            3392..4132
                     /locus_tag="WIB82_000890"
     CDS             3392..4132
                     /locus_tag="WIB82_000890"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ3842209.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            4170..5093
                     /gene="cysD"
                     /locus_tag="WIB82_000891"
     CDS             4170..5093
                     /gene="cysD"
                     /locus_tag="WIB82_000891"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ3842210.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPAEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            5132..6418
                     /locus_tag="WIB82_000892"
     CDS             5132..6418
                     /locus_tag="WIB82_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ3842211.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKEAIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ENLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVEQTVRIEPNG
                     LTAKVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARRYLLKHGTQTLPAKVGEIESVLDVLTLEQEAGAESLKINDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            6415..8229
                     /locus_tag="WIB82_000893"
     CDS             6415..8229
                     /locus_tag="WIB82_000893"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ3842212.1"
                     /translation="MSGHDMQNTNPPLPPMPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLKAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLIERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTVPEGRYSKADPFPAALLANQKITARQSDKDVRHIELDLSGSDLSYQ
                     AGDALGVWFDNNPALVGEILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDKSKPIVMIGSGTGIAPFRAFLQQRAAENATGKNWLFFGNPHFARDFLYQTEWQ
                     QFAKEGFLHKYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            8256..10025
                     /gene="cysI"
                     /locus_tag="WIB82_000894"
     CDS             8256..10025
                     /gene="cysI"
                     /locus_tag="WIB82_000894"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ3842213.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            10198..12057
                     /gene="ilvD"
                     /locus_tag="WIB82_000895"
     CDS             10198..12057
                     /gene="ilvD"
                     /locus_tag="WIB82_000895"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ3842214.1"
                     /translation="MPAYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEQWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(12120..12380)
                     /locus_tag="WIB82_000896"
     CDS             complement(12120..12380)
                     /locus_tag="WIB82_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ3842215.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            12703..13338
                     /locus_tag="WIB82_000897"
     CDS             12703..13338
                     /locus_tag="WIB82_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ3842216.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQVGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            complement(13416..>13829)
                     /locus_tag="WIB82_000898"
     CDS             complement(13416..>13829)
                     /locus_tag="WIB82_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ3842217.1"
                     /translation="TRAERAGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWT
                     EFVRTIAVARITMPQSYVRLSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDED
                     GDRILMEKLNLYPLQFEPEGEVAEVEKAFGIKVDY"
BASE COUNT         3303 a         4160 c         3685 g         2681 t
ORIGIN      
        1 tgtactggtt tttgttaatc cactatatct tcttacatac ttttagttat ccggttgcgc
       61 agccaccttg ccgccggagc gatttcccct gccaacagcc ctgccgcaat gcctgccgat
      121 tcttttatga catccgccgc cgcgccgtgc agccacgcgc ccgcgcaggc ggcttcaaaa
      181 accggcacgc cctgtgcgag cagactgccg atgatgccgc ccaatacgtc gccactgccc
      241 gccgttgcca atcccgcgtt gccgctttcg ttgacataga tttccgtatc gggtgaggca
      301 accaatgttt tgtgcccctt taaaaccacg gttgcgccga aaattgcccc tatcttcctc
      361 actgccgccg tccgatccgc ctgaacctgc gcaaccgtcg ttccaagcag gcgcgcggct
      421 tcggcggggt gtggcgttaa aatcaggttt ttacacccgc gcgccagatt tcgggtttcg
      481 gcatcggttg ataatatgtt cagcgcatcc gcatccaaaa cgacgggctt gtccgtgtgt
      541 tccgtcaaaa ttccggcaag cgttccgacc gccgccctac ctgtacccaa tccacaaccg
      601 acaacccagg cgtttatatc ttgacgtttg gccaaactgt ccgccgtatc cagcataatc
      661 tcgggaaaac cggcaataac ggcaaaaggt agcgtatcct gattgaaacc cgcccacact
      721 ttgccgcagc cgagatacat tgccgccgat gccgccaata cgggcgcgcc gctcatccct
      781 gccgatccgc cgactacggc gagcgtgccg aaagtccctt tgtgcgaatc ttccgcacgg
      841 gctttgaaaa catggggaaa acgcaatgcc gtctgaagca tctggcggcg gctctcgcct
      901 gaaagatgaa aaacgggaaa cattgcccct ccaatcggtt aaaataaggc tcgaaaacaa
      961 accgattcta ccgccggaaa ccgattatga aaaacaaaac caaagtctgg gacctcccca
     1021 cccgcctttt ccactggctg cttgccgcgt ccctgccctt tatgtggtat agcgcgaaag
     1081 ccggcggcga tatgctgcaa tggcacacgc gcgtcgggct gttcgtcctt ttcctgctcg
     1141 tatttcgcct ctgctggggc atttggggca gcgataccgc ccgtttttcc cgtttcgtcc
     1201 aaggctgggc aggcatacgc ggctatctga aaaacggtat tcccgaacac atccagcccg
     1261 gacacaaccc cttgggcgca ctgatggtcg ttgcgctttt ggccgccgtg tccttccaag
     1321 tcggcaccgg gctttttgcc gccgatgaaa acaccttcag caccaacggc tacctcaacc
     1381 atttggtttc cgaacatacg ggcagcctta tgcggaaaat ccacctcaac tttttcaagc
     1441 tgctcgccgt tttttctgca atccacatcg ccgccgtcgc cgcataccgc gtattcaaaa
     1501 agaaaaacct catcctcccg atgataaccg gcttcaaata catcgaaggc aaaacctcaa
     1561 tccgctttgc aggcaaagcc gcgcttgccg ccgcattatc ggttgcctcg cttgccgcag
     1621 ccgccatcct gctcctgtcc tgaaacccga catcaatgcc gtctgaagcc cttttcgccc
     1681 ttcggacggc atcccctcac cacgcaaacg gtctgaatgc ccgaaacggc ttcaggccgt
     1741 ttttcttccg aaaccgttat aaaacggaaa accgccgcat ccgcagccgc ccccatatag
     1801 ctttcagctt ctaacaaata cctttttatt atttaaccgg ggaaattctt ttgtctaaaa
     1861 tgactcgcgt tccgacaaaa atgccgcgac gcaccgtatc ggaaacaagg tgcgggcaaa
     1921 cagcaaagga gtcagccagt gaattatttc cccattttcg ccaacctcgc cggccgcccc
     1981 gtattggtcg taggcggcgg cgcagtcgcc gcacgcaaaa tcagcctgct gctgaatgcg
     2041 ggcgcagagg tcagggttgc cgcaaaacac ctgaatgccg aactctccgc gttggcggcg
     2101 gaaaacaaaa tcctgtggct tgccgaagaa tttcgtgccg aacacatccg cacggttttc
     2161 ctcatcattg cggcaagcag cgaccaagcc ctcaaccggc gcgttttcca tcttgccgaa
     2221 agctgccaaa agccggtcaa cgtggtggac gaccgcgacc attgcagctt catcttcccg
     2281 tcggttatcg accgcaaccc ggttcagatt gccgtttcca gctccggcag cgcgcccgtc
     2341 ctcgcccgac tgctgcgtga aaggctggaa gccctgctgc ctcattcttt gggcgatatg
     2401 gcggaaatct ccggaagatg gcgcgatgcg gtcaaagcca aactgaaatc cgttaccgaa
     2461 cgccgccgtt tttgggaaaa gcagttcaac ggccgttttg ccgccctcgt taaaaatcaa
     2521 caaaccgccc aagccgaaca ggagttggca aagcagttgg aacaaaatta tcaaggcggt
     2581 tttgtgtctt tggtcggcgc gggtcccggc gatgcggggc tgctgacgct caaagggctg
     2641 caggaaatcc agcaggcaga cgtggtgctt tacgacgcgc tggtttcaga cggcatactg
     2701 tccctcgtcc gccgcgatgc ggaacggatt tttgtcggca agcgcgcccg gggcgaccgc
     2761 accccgcaag aggacaccaa tgcgcttatg gtcaggctgg cccgcgaagg caggcgcgtg
     2821 gtgcgtttga aaggcggaga ccctttcgtg ttcggacgcg gcggcgaaga attggaaacc
     2881 ttggcacgcc atcagattcc gttttcggtc gtccccggca ttaccgccgc cgtcggcgcg
     2941 accgcctatg ccggtatccc gctcacgcac cgcgattatg cccaaagtgc ggttttcgta
     3001 accggccacc gcaaggcgga tgcgcccgac atcgaatggc agaccctcgc ccgcagccgc
     3061 cagaccttgg tgatttatat gggtgcgctc aaagccgccc tgattgccga acggctgcaa
     3121 cagcacggac gctcgcccga cacgccggca gccgtcatca gccaaggcac gctgccggct
     3181 caaaaaaccg caaccggcac gctcgccaac cttgccgaac tcgccgaaac cgcgccgaat
     3241 ccggcattga ttgtcatagg cgaagtggtg gggctgcacg aaaaactcgc ctggttcggt
     3301 gaaaacggcg aaggagaaaa ccgggtcggg caggcgtatc ccgcattggg cggactgaat
     3361 gccggacaac gggcggcata aggcgggaaa aatggaaacg acactgttca aacccgccct
     3421 gtggcagata ccgcatatcg gatccggcgg cgaaaccgcc cttgcggaaa aaacggaaac
     3481 cctcaaacaa cgcctgcacc ggattgtcgg cagccaccgg gatgcacggt ttgccagcag
     3541 ccttgcggca gaagatatgg tgattaccga ccttatcgcc ggcgaaaacc tcaatatcgg
     3601 catttttacc ttggatacgg ggctgctcca cacggaaacc ctgaacctgc tggacaggct
     3661 cggacgcgca tatccccatt tgcggataaa gcgtttccgg ccggttcgag aagatgccga
     3721 ccgttatgtg gaaagcaaag gcaggtttgc cttttacgac agcgttgaag cgcgccgcga
     3781 atgctgccgc atccgaaaaa tcgaaccgct caaccgcgcc attgcaggcg cggacgcctg
     3841 gcttaccggg caacgccgcg aacaatccgc cacacgcacg gaactgccgt ttgccgaata
     3901 cgatgccgga cggggcatcg gcaaatacaa cccgattttc gactggtcgg aacacgacgt
     3961 gtgggcatac atcctcgcca acaatgtgcc ttacaacgat ttgtaccggc aaggatttcc
     4021 cagcataggc tgcgacccct gcacccgccc cgtcaaggcg ggcgaagaca tccgcgccgg
     4081 gcggtggtgg tgggaaggca gaaacagcaa agaatgcggg ctgcacaaat aattcataaa
     4141 tatcagacag aagaaataag gaatatggta tgacgaaaac cgaaccgaac aacgcccaac
     4201 tcgactggtt ggaatccgaa tccatccaca tcatccgcga agtggcggcg gaatgcgaaa
     4261 accccgccct gctcttttcc ggcggcaaag attccgtggt cctgctcgcc ctcgcctgca
     4321 aagccttccg gctgggcagc aggccggtaa aactgccctt cccgctggtg catatcgaca
     4381 cggggcacaa ctaccccgaa gtgattgctt tccgcgacgc gcaggcggca aaactcaatg
     4441 cccgcctgat agtggggcgc gtggaagact ccattgccaa aggcacagta gttttacgca
     4501 aagaaaccga ttcgcgcaat gcggcacagg cggttacctt gttggaaacc atcgaggcaa
     4561 acggctttga cgcgctgatg ggcggcgcgc ggcgcgacga agaaaaagcc cgcgccaaag
     4621 aacggatttt ctcgttccgc gacgagttcg gacaatggga tccgaaggcg caacgccccg
     4681 agctgtggtc gctgtacaac accaggctgc acaaaggcga aaatatgcgc gtcttcccga
     4741 tttccaactg gacggaactc gacatctggc aatacatcgc ccgcgaaaac ctcgagctgc
     4801 cgccgattta ttacagccac aggcgcgaag tggtcagacg cagggggctg ctggtccctg
     4861 taacgccgct caccccgaaa atgccgtctg aaacctccga aatccttgat gtccgcttcc
     4921 gcaccgtcgg cgacatcagc tgcacctgcc cggtagaaag caccgcgtcc acgccggcgg
     4981 agattatcag ggaaacggcg gttgccgaca tttccgaacg cagcgcgacc cggctagacg
     5041 atcaggcaag cgaggcggca atggaaaaac gcaaaaaaga aggctatttc taaatttcaa
     5101 aaacccttta agcagatatg aggaagaaaa tatgacggca caacaccagg ccccgctccg
     5161 cttcattacc gccggcagcg tcgatgacgg caaaagcacc ctgatcggac gactgctcta
     5221 cgacagcaaa gccctgctgt ccgaccaaat caaaaccttg gaatccggca aaagcaaagg
     5281 tttgaaagaa gccatagact tctccatcct gaccgacgga ctggaagccg aacgcgaaca
     5341 gggcattacg atcgatgtgg cataccgcta tttttccacg gcgaaacgga aattcatcat
     5401 cgccgacacg ccggggcacg aacaatacac gcgcaatatg gttacgggcg caagcaccgc
     5461 ttcggcggca gtcgtgctgg ttgacgcatc ccaactggat ttcggcgcgc agcccttgca
     5521 gcttctgccg cagaccaaac gccattccgc catcctccgc caactcaact gcccgcatat
     5581 tgtggtggcg gtcaacaaaa tggacctgct ggattacagc gaagacaaat tcaacgccat
     5641 tgtggaggca taccgccgac ttgccgaaca gctcggtttg aaagacgtgc atttcgtacc
     5701 ggtgtccgca ctcttgggcg acaacatcgt ttaccccggc ggcaatatgc cttggtataa
     5761 aggcgaaccc ttattgtcca ttttggaaaa cctgccgggg gcggacgaag tcagccgcac
     5821 cgccgacgat ttttatttcc ccgtgcaact ggtcgtccgc caagatgccg acaaggccga
     5881 tgatttccga ggctatcagg ggcgcatcga acgcggttcg gtcgccgtcg aacaaaccgt
     5941 ccgcatcgaa ccgaacgggc tgaccgccaa agtgtctgaa atcatcacac ccaaaggcga
     6001 agtggcgcag gcttttgccg gcgaggcggc aaccctccga ctggatcgcg acatcgatgt
     6061 ttcgcgcggc gacctttttg tcgataaaaa ttccccgctc gccccgcaaa aacatctgga
     6121 agccacgctt tgctggtttg acgaacgtcc gctcaacacc gcgcgcagat acctgctcaa
     6181 gcacggcacg caaaccctgc cggcaaaagt cggggaaatt gaaagcgttt tggatgtcct
     6241 cacgctggaa caagaggcag gcgcggaatc cttgaagata aacgacatcg ccaaagtccg
     6301 catcaacctt caaaaacccg ttacggcaac gccttatgcg gaaaacaccg ccgccggttc
     6361 gtttatcctg atagacgaag cgacatacgg cactgttgcg gcaggtatga ttttatgagt
     6421 ggacacgata tgcagaacac aaatccgcca ttaccgccta tgccgcccga aatcacgcag
     6481 ctcctgtcgg ggctggacgc ggcacaatgg gcgtggctgt ccggctacgc ttgggcaaaa
     6541 gcaggaaacg gggcatctgc aggactaccc gcgcttcaga cggcattgcc ggcggcagaa
     6601 cccttttccg ttaccgtcct ttccgcctcg caaaccggca atgcgaaatc cgttgccgac
     6661 aaagcggcgg acagcctgaa agccgccggc atacaagtca ggcgcgccga actgaaagac
     6721 tacaaggcga aaaacatcgc agacgaacgc cgcctgctgc tggttacgtc cactcagggc
     6781 gaaggcgaac cgccggaaga agccgtcgtg ctgcacaaac tgctgaacgg caaaaaagcc
     6841 ccgaaattgg acaaactcca atttgccgta ctgggcttgg gcgacagctc ctatccgaat
     6901 ttctgtcagg caggtaaaga tttcgaccgg cgttttgaag aattgggcgc aaaacgcctg
     6961 atcgaacgcg ttgatgcgga tttggacttt accgcctctg caaacgcctg gacagacaat
     7021 atcgccgcac tcttaaaaga agaagccgca aaaaaccggg caacacccgc gccgcaggca
     7081 acgccccccg ccggccttca gacggtaccg gagggcaggt actctaaggc agaccccttt
     7141 cccgccgccc tgctggccaa tcagaaaatc accgcccgcc agtccgataa agacgtgcgc
     7201 cacatcgaac ttgatttgag cggttcggat ttaagctatc aggcaggcga cgcgctcggc
     7261 gtttggtttg acaacaatcc ggcactggtc ggggaaatcc tagacctgct cggcatcgat
     7321 ccggcaacgg aaatacaggc aggcggaaag atgatgccgg ttgcgcgcgc actttcatct
     7381 catttcgaac tcacgcaaaa caccccggct ttcgtcaaag gctatgccgc gttcgcccat
     7441 tatgaagaac tcgataaaat cattgccgat aacgccgttt tgcaggattt cgtgcaaaac
     7501 acgcctattg tcgatgtgct gcaccgcttc ccggcaagcc tgacggcaga acaattcgcc
     7561 ggcctgcttc gcccgcttgc gccgcgcctg tattcgattt cctcgtcgca ggcggaagtg
     7621 ggggacgaag tgcacctgac cgtcggcgcg gtgcgttttg aacacgaagg gcgcgccagg
     7681 gcgggcggcg catcgggttt ccttgccgac cggctggaag aggacggcac ggtgcgtgta
     7741 tttgtggagc gcaacgacgg cttcagactg cccgaagaca aaagcaaacc cattgtaatg
     7801 ataggctccg gtacgggcat tgcaccgttt cgcgcttttt tgcaacaacg cgccgcagaa
     7861 aatgcaacag gcaaaaactg gctgtttttc ggcaatccgc attttgcccg tgattttctc
     7921 tatcaaaccg aatggcagca gtttgccaaa gagggtttcc tgcataaata cgatttcgcc
     7981 tggtcccgcg atcaggaaga aaaaatctat gtgcaggaca aaatccgcga acaggcggaa
     8041 ggactttggc aatggctgca ggaaggcgcg catatctatg tgtgcggcga tgcggcaaaa
     8101 atggcaaaag acgtggaagc cgccttgctg gatgtgatta tcggggcagg acatttggac
     8161 gaagagggcg cagaagaata tttggatatg ctgcgcgaag aaaaacgcta tcagcgtgat
     8221 gtttattgat taaatataat cgggaggaac acaaaatgac cgtacagacc aagacaaaag
     8281 gtttggcgtg gcaagaaaaa ccgctatccg acaacgaacg tctgaaaacc gaaagcaatt
     8341 ttttacgcgg cacgattttg gacgatttga aagacccgct cacgggcggc ttcaaaggcg
     8401 acaacttcca actcatccgc ttccacggta tgtatgagca ggacgaccgc gacatccgcg
     8461 ccgaacgcgc cgaggcaaaa ctcgagccct tgaaatttat gcttttgcgc tgccggctgc
     8521 cgggcgggat catcaaaccg tcccaatgga tagaactgga caaatttgcc cgggaaaaca
     8581 gtcattaccg ctccatccgg ctgaccaacc ggcaaacctt ccaatttcac ggcgtgccga
     8641 aagccaagtt gcagacgatg caccgcctct tgcacaaact gggtttggat tccatcgcca
     8701 cggcggcgga tatgaaccgc aacgtgcttt gcacgtccaa cccgatcgag tccgaactgc
     8761 accggcaggc ttacgaatac gcgaaaaaga tttccgaaca cctgctgccg cgcacgcgcg
     8821 gttatctgga tgtgtgggtg gacggcaaaa aagttcaaag ttccgacgac ttccttcaag
     8881 aagacgagcc gattttgggc aaaacctatc tgccgcgaaa attcaaaacc gcagtcgtca
     8941 tcccgccctt gaacgatgtg gactgctacg gcaacgattt ggatttcgtc gccgtttcag
     9001 acggtaacgg acagcttgcc ggcttcaatg ttttggcagg cggcgggctt tcgatggaac
     9061 acggcaacac caaaacctat ccgaacattt cactggaact gggtttcgtg cctccggaac
     9121 acgcgctgaa ggccgccgaa gcggtggtaa ccacgcagcg cgacttcggc aaccgcagcg
     9181 accgcaaaaa cgcccgcacc cgctacacca ttcaaaatat gggcttggac aacttccgcg
     9241 cggaagttga acgccgtatg ggtatgccgt tcgaacccgt acgcccgttc aaatttaccg
     9301 ggcgcggcga ccgcatcggc tgggtgaaag gcatagacgg caactggcat ttaacccttt
     9361 tcatcgaaag cgggcgtttg gttgacgaag gcgggaaaca gcttctgacc ggcgtgttgg
     9421 aaatcgccaa aatccacaaa ggcgatttcc gcatcaccgc caaccaaaac ctcatcgtgg
     9481 caaatgtcgc cgaagcagac aaagcaaaaa tcgaagaatt tgcccgaaca tacggattaa
     9541 tccgcaacga tgtcagcaag ctgcgtgaaa atgcgatgtc ctgcgtttcc tttccgacct
     9601 gcccgctggc aatggcggaa gccgaacgcg tgctgccgga cttcatcggc gagctggata
     9661 agattatggc gaaacacggc acgtcggacg actacatcgt tacccgcatt accggctgcc
     9721 cgaacggctg cggacgggcg atgttggcgg aaatcggact ggtcggcaaa gccgtcggac
     9781 gctacaacct ccatatcggc ggcgaccgtg aaggcgtacg catcccccgt ctttacaaag
     9841 aaaacatcac cctgcccgaa atccttgccg aattggacga cctgatcggc aaatgggcgg
     9901 cagaacgcaa tatcggcgaa ggtttcggcg atttcgccat acggacgggc atcgtcaaac
     9961 ccgtattaaa tgcacccgtt gatttttggg acgcatccaa agccgtcgcg attgcccgcg
    10021 cctgaacccg tccccgcatt cctgcgggga ttttcttttt tgccgcacgc cgtttcaatc
    10081 ccgcaaaatt ccgatacttg gtataaaata ccctcttttc ccactctaaa aaaccgtacc
    10141 cgataccgtc tgaaacagcc ctgtctttca gacggtatag gcacaaggaa acacactatg
    10201 cctgcatacc gctccaaaac ctccacccac ggccgcaata tggcgggcgc acgtgcattg
    10261 tggcgcgcca ccggcgtgat ggaaaccgac ttcggcaagc ccatcatcgc cgttgccaac
    10321 tccttcaccc aattcgtgcc cggccatgtc cacctgcaca atatgggcca gctggttgcc
    10381 cgcgaaatcg aaaaagccgg cgcaatcgcc aaagaattca acaccatcgc catcgacgac
    10441 ggcatcgcta tgggacacag cggcatgctg tactccctgc ccagccgcga tttgattgcc
    10501 gactctatcg aatatatggt caacgcccac tgcgccgacg cgctggtgtg catttccaac
    10561 tgcgacaaaa tcaccccggg catgctgatt gccgcgatgc gcctgaacat ccccaccatc
    10621 ttcgtctccg gcggcccgat ggaagcgggc aaggttatcg gcgtggcaaa catccagccc
    10681 gaacgccgtt tggacttgat tgacgcgatg attgaatcgg cggacgacaa tgtcagcaac
    10741 cggcaagtcg aggaagtcga acaaaacgcc tgcccgacct gcggctcgtg ttcgggtatg
    10801 tttacggcaa actcgatgaa ctgcctgacc gaagcactcg gcctttccct gcccggcaac
    10861 ggttcgtatt tggcgaccca cgccggccgc aaagaattgt tcctcgaagc cggccgtatg
    10921 attgtcgaaa tcaccaaacg ctattacgag caaaacgatg aaaccgtgtt accgcgcagc
    10981 attgccacca aaaaagcgtt tgaaaacgct atgacgatgg atattgcgat gggcggcagc
    11041 accaatacca ttttgcacct gttggccgtt gccaacgaag ccggtgtcga tttcaaaatg
    11101 gcagacatcg accgcttaag ccgcgtcgtg ccctgcatct gcaaaaccgc acccaacaac
    11161 cacgactact atatggaaga cgtgcatcgc gccggcggta tcttcgccat cctgaaagaa
    11221 ctggacaaag cgggcaaact gcacaccgac gtgcacacca tccacgcgcc gacgctgaaa
    11281 gacgcgattg aacaatggga cgtgaccaat cccgaaaaca cccgtgccat cgaacgcttc
    11341 aaagccgcgc cgggcggcgt acgcaccacc caagcgttct cacaaaaccg tatgtggaaa
    11401 accctcgacc tcgaccgcga aaaaggctgt atccgcgacg tggcacacgc ctactcgcaa
    11461 gacggcggtt tggcggtctt gttcggcaac atcgccgagc gcggctgcgt ggtaaaaacc
    11521 gcaggcgtgg acgagagcat cctcaaattc accggccgcg cccgcgtgtt tgaaagccaa
    11581 gaagacgcag tagaaggcat tttgggcaac caaatcgtcg ctggcgacat cgtcatcatc
    11641 cgctacgaag gcccgaaagg cggcccgggc atgcaggaaa tgctgtatcc gacttcctac
    11701 ctgaaatcta aaggcctcgg caaagcctgc gctctcttaa ccgacggacg cttctccggc
    11761 ggcacatcag gtttgtccat cggacacgcc tcgcccgaag cggcggaagg tggcgcgatc
    11821 ggtttggtac acgaaggcga taccgtcgaa atcgacatcc ccaaccgcag catccacctt
    11881 gccatttccg atgaagagct tgccgcacgc cgtgccgaaa tggaagcgcg cggcagcaaa
    11941 gcatggaagc ctaaaaaccg cgaccgctac gtctccgccg cattaagagc ttacggcgcg
    12001 atggcgactt ccgccgacaa aggcgcggtg cgcgacgtag cgcaaatcga aagataagcc
    12061 ccaaaacgtg cggcaaatgc tgtctcgtct gaacagtaaa ggtctgatga tggacaaatt
    12121 tatctgggcg atggcggtat tttccgcaat tttggcctcg ttatcggcag cggctttagc
    12181 gcgatcgagt atgtaaaaga gctgccgctc gccgccgttg ccgtgatgcc ttgcgcgccg
    12241 aagtcgttgc tgtggttgtt ggcgaagctg aggtagtcga atcccgcgtc ggcaaggtat
    12301 tgcccgtatg cggagggcgt tcggaatgca tagcatattt tgggggtttg cacatttttt
    12361 cggcgtaccg ccttcgtcaa acagcgtgcc ttcgaggttg ccgacggtaa tgtccgcgtc
    12421 ttgcaaggca gattcgacgt ttttcagaat attggtatcg ggcaggtaat cgaccggata
    12481 attgctgccg agcataatgt cgcccacgcc gataatggaa acggtatcgg ctgtcttttc
    12541 gtcaggcgca tcctgtccgt ccacatcgcc ggtatttgaa acgggcgcgg ggtttgaacc
    12601 gtcaatcggc agcacgggga ttccgttccg gcaggatgcg ccgagggctt gaccccggcg
    12661 gtttgcggtg cggaaacggc ataaagggcg aaccttgacg aattgaacat caaatattcg
    12721 ggcatcgaaa acaggtttga aaccgccatc ctgaagaaaa acggcgtaag atccggcttc
    12781 gtctccttcg cccccaacct tgccgccgtc aaactgaacg attatgccaa agttagaaaa
    12841 cggattacca aaaccaaaca gaaagccgac atcgtcattg tgatgttcca cggcggcgcg
    12901 gaagggaaac aggcggaaca cctgccgttc gataccgaaa tcttctatgg ggaaaacagg
    12961 ggcaacgtcg ttgagtttgc gcggcttgcc gtcgattccg gcgcggatgt cgtattcggg
    13021 caggggccgc acgttactcg cgccgtcgaa ctttaccgcg accgcttcat ctcctacagc
    13081 ggcggcaact ttgccaccta cggcgcaatc aacacctccg gcatcagcgg catcgccccc
    13141 atttttaaaa ttatcaccga caaacagggg cggttcgttt ccggcaacat tatccccatc
    13201 actcaagtcg gcgataaaat ccccaaaatc gaccccgaga aaaccgttat cgggcggatt
    13261 atttatctga accgcagcga tttccccaag gggaacgggc tggatgtctc gcccgacggc
    13321 agcatcacgc gccggtaaaa agatattgga aatgccgtcc ggacaaatgc cgtctgaaag
    13381 cctttttccg ggtttcagac ggcatttttc aatcgtcaat aatccacttt aatcccaaag
    13441 gctttttcca cttcggcgac ctcgccttcc ggttcaaact gcaagggata caggttgagc
    13501 ttttccatca ggattctgtc gccgtcctca tcaggattgc ccgtggtcag cagcttgtcg
    13561 ccgtaaaaaa tcgagttcgc gcccgccata aagcacatcg cctgcattgc ttcaggcata
    13621 ttgctgcgcc ctgccgacag ccggacataa ctttgcggca tcgtaatccg cgccacggcg
    13681 atggtgcgga caaattccgt ccagtccaaa tcttcggcat cggcaagcgg cgtgccttcc
    13741 actttgacca accggttaat cggcacgctt tcgggctgcg ggtcgagatt ggcgagactg
    13801 gcaatcagcc cggcacgttc ggcgcgggt
//
LOCUS       DAWHRS010000044        13824 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWHRS010000044 DAWHRS010000000
VERSION     DAWHRS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13824
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.044"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..671)
                     /locus_tag="WIB82_000899"
     CDS             complement(<1..671)
                     /locus_tag="WIB82_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ3842218.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFR"
     gene            856..2283
                     /locus_tag="WIB82_000900"
     CDS             856..2283
                     /locus_tag="WIB82_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ3842219.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLGRYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDAAACLLNDIRKDILDCRYCG"
     gene            2325..2576
                     /locus_tag="WIB82_000901"
     CDS             2325..2576
                     /locus_tag="WIB82_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842220.1"
                     /translation="MPLDGIFGGGRQISGGAKGNARHSAKPPIAADTAFRAAPLRRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            2485..3594
                     /gene="mltB"
                     /locus_tag="WIB82_000902"
     CDS             2485..3594
                     /gene="mltB"
                     /locus_tag="WIB82_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ3842221.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(3717..5723)
                     /locus_tag="WIB82_000903"
     CDS             complement(3717..5723)
                     /locus_tag="WIB82_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ3842222.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGSLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            6012..8039
                     /locus_tag="WIB82_000904"
     CDS             6012..8039
                     /locus_tag="WIB82_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ3842223.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGELGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGNLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(8130..9683)
                     /locus_tag="WIB82_000905"
     CDS             complement(8130..9683)
                     /locus_tag="WIB82_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ3842224.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(9932..11125)
                     /locus_tag="WIB82_000906"
     CDS             complement(9932..11125)
                     /locus_tag="WIB82_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ3842225.1"
                     /translation="MNPKHLIAFSALFAATQANALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRSAAADFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(11122..12105)
                     /locus_tag="WIB82_000907"
     CDS             complement(11122..12105)
                     /locus_tag="WIB82_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ3842226.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPK
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAARAHHVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLATKIMEKIVFEPSTQPSSTQP"
     gene            complement(12116..13552)
                     /locus_tag="WIB82_000908"
     CDS             complement(12116..13552)
                     /locus_tag="WIB82_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ3842227.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDREGTRRFWLGCGIAASLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHRPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWLITFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            complement(13681..>13824)
                     /locus_tag="WIB82_000909"
     CDS             complement(13681..>13824)
                     /locus_tag="WIB82_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ3842228.1"
                     /translation="NVGIGGGALLGHWVTQYSGISCIGVAGMLTAAAGLWVCLNLNRY
                     IRT"
BASE COUNT         2947 a         3480 c         4227 g         3170 t
ORIGIN      
        1 ctgcggaaga ttttttgcgc ttcgctgtcg gtggggaact cgtgcgccgg cagcaggcgg
       61 atttcggaaa caggagaaat ggtgcgttgt gtttcggtat cgaaagtttt gatgctgtcg
      121 atttcatcgt cgaacaaatc aatgcggtac ggcatttcgc tgcccatcgg gaacaaatcg
      181 acaataccgc cgcgcacagc gaactcgcct gccgcgacaa cgtgggaaac atggttataa
      241 cctgcatcca ccaaatcgct tttcagacgg cctatatcca aagtctgccc tgttttcagc
      301 caaaacgtgc gccccgccag aaacggcaca ggcggcagct tctgcatcgc cgtggcaacc
      361 ggcacgaaca acacatccgc cgcgccgctt ttaatctgcc acaacgccga cagccgctcc
      421 gacaccaaat cctgatgcgg cgaaaaacgc tcgtaaggca gcgtttccca gtccggcagg
      481 aacaccgccg tgtcgtgcgg acggaaaaac cgccatgccg tctgaaggcg caacgcctgt
      541 tccgcatctt gggtcagcac gaccttgagc cgcttgtgcg gcagataacg cgccaaagcc
      601 aagggcagcg agccttgcga aagattgggc caacgggatt tttcacgggg tttggggatg
      661 gggtaggtca tagtgcggca atcggcggac agtgatgcaa acggtcattt tagcacggga
      721 gggggcgcga tgccgtctga aaggtcggcg gattgttgcc cggcgcactt tgtggtaaag
      781 tttagaaaat caactctaaa ctgtggcagg cggtttgacc ggccgccgat taatgggaac
      841 cgacagagga agccgatgat acacaccgaa ccgagcgcgc agccgtcaac tatggacacg
      901 gctgcttttt taaagcacat cgaatccgca ttccgccgca ttttttcaga cggtatcgac
      961 ctgatgcgat acctgcccga agacaaatgg cttgccttga agcaggcggg tttgctgttg
     1021 cccttcctcg acaaaaaaca cggcgggcgc aagggcagcc agtttgaaat tcaggaagtc
     1081 ttgcggattg cggggcatta cggcgtgccc gttacgctgc gtaccggcat cgaaggcgcg
     1141 ctggtgttgc agccactgca agagttcggc gatgaagcgc aagtcgcgca aggtttggag
     1201 atgattttca aaggcgaggg cggcggtttg ggcgttaccg aacccgaaac ctccggcgcg
     1261 gcgattgcac gcgaaatgca gtcttactac gaatataccg acggacaaac catttacgtc
     1321 aacgccgcga aatactggca gggcaactcg caaagcgact tcctcctcgt tgccgccaaa
     1381 gagcgcaaaa acggcaaact cgccaaagtc atcgacctgc tgctcgtccc caaaacatac
     1441 atccgctgcg aaaccctcgc atccgaaggc ttgcgcgccg tccgttacgc cgtcaaccgc
     1501 atcgatgccg aaatgcctgc aaccgccgtg atgaaactct cccagagcga cgctgccggt
     1561 ttgcgcgcgt tccaaaacat ctttatccgc agccgcctgc aactgatcgg catgacgcac
     1621 ggcattatgg aatacatcct tgaaaacctg ggacgatacg tccgcaacga catcagattc
     1681 gtcgattacg aacgccgcga aatccggcgc cgccatcagg tttccgagat tctttaccgc
     1741 tacgtctgcc attccgtttc gcctgttgcc cccgtcgccc atcagctgat ggaggcgaac
     1801 atcgtcaaaa ccctcgccac ggaatacact tatgccgccg cgcaaatgtt gcaaaaactc
     1861 ttgggcgcga agggttttga acgcggacac accgccggca atatcgccat cgacatccgt
     1921 cccttcacga tttttgaagg cccgaacgat atgctttatg ccgaaattta cgaccagttt
     1981 gtccgcgcca ccgccgaaga aaaagaagca ggcatgaagt tggacaaaaa ccaaaccctg
     2041 ctcgaccgcc tgcaaaccga tgcccgcttt gccgccgtcg cccgcgacta cactttgcct
     2101 gaagacatcc gcagcttcct gcaggaacac accctgaccg atgcctgcgc cctgcaaaaa
     2161 gtctttatcg gcaaaatcat cgcccgactc tttgtcttcg tacaggcgaa acacgaagac
     2221 gcggcagcct gcctgctgaa cgacatccgc aaagacatat tggactgccg atattgcgga
     2281 tagcgggcaa atacggcggc aggcaatgcc gtctgaaagg ggaagtgcct ttagacggca
     2341 ttttcggcgg cggcaggcag atttcgggcg gcgcaaaagg caacgcgcgg cattctgcaa
     2401 agccgccgat tgcggcggat accgcatttc gcgccgcgcc cttgcgccgc tttcccactt
     2461 accctatttg ttcggaacat atttatgaaa aagagaaaaa tactgccgct ggcaatttgt
     2521 ttggcggctt tgtctgcctg tacggcgatg gaggcacgcc cgccccgggc aaatgaagcc
     2581 caagcccccc gcgcggatga aatgaaaaaa gaaagccgcc ccgcgtttga cgcggcagcc
     2641 gtatttgacg cggcagccgt accggtatcc gacagcgggt ttgccgccaa tgcaaatgtc
     2701 cgccgttttg tggacgatga agtcgggaaa ggggattttt cccgggcgga atggcaggat
     2761 ttttttgaca aagcggctta caaggcggac atcgtcaaga ttatgcaccg cccctccaca
     2821 tcgcgtccgt ggtatgtgtt ccgcacggga aattcgggca aggcgaaatt tcgcggcgcg
     2881 cgccggtttt atgcggaaaa ccgcgcgctt atcgatgatg tggcgcaaaa atacggcgtg
     2941 cctgccgaac ttatcgtggc ggttatcggg attgaaacga attacggcaa aaatacgggc
     3001 agtttccgtg tggcggacgc attggcgacc ttaggctttg attacccccg ccgcgccggg
     3061 tttttccaaa aagaattagt cgagctttta aagctggcaa aagaagaagg cggcgatgtt
     3121 ttcgccttta aaggcagcta tgcgggcgca atggggatgc cgcaatttat gccttcgagc
     3181 taccggaaat gggcggtgga ttatgacggg gacggacatc gggacatatg gggcaacatc
     3241 ggcgatgtcg cggcatcggt tgccaattat atgaagcagc acggttggcg cacgggcggg
     3301 aaaatactgg tgtctgcaac attggcgccg ggtgcggatg ttcaggcaat cattggcgaa
     3361 aaaaccgccc tgacgcggac ggtggcggat ttaaaggcgt acggcatcat ccccggcgaa
     3421 gagcttgcag atgatgaaaa ggcggttttg ttcaaactgg aaaccgcacc cggcgtgttt
     3481 gaatattatt tgggcttgaa caatttttat acggtatggc agtacaatca cagtcggatg
     3541 tatgtaacgg cggtcaggga cattgccaat tcgcttggcg gcccgggatt gtaatgtttt
     3601 ttaaaatgcc gtctgaacca cgtttcggtt cggacggcat ttttgcatcc ttttatgggg
     3661 atttcccgca tcagggcggt attcggggcg aatcccgctt cagacggcat tgccggtcaa
     3721 ggcttgtccg aaggaggatg tccctgttct ttggcgggcg ggaggaaaag tatgcaggga
     3781 ttgcttttaa tctgattcca aaaactttta atcagattgc ggatgctgcc gattttcgtg
     3841 ccgcgcgagc gcagggcgac gaagagggcg aggctgaagc tgacgcagag gttgaccagg
     3901 ccgatggcga ggacgctgaa aatgccgagt acgaacgtgc ccaaaccgac gttgccgctg
     3961 acggcggcat agccgaggtt ggcggaggaa aaggcgacgt ggcggatgtc cagcggcagc
     4021 ccgaggaggt gtccgaaata gccggtcata cccaagagca tcccgaagat gaagttgccg
     4081 accagcgagc cgtagtgttt gtggatgtag gcggcgagga cgcggcgggg cccggggcgc
     4141 atgattttac gcagcaaggg gttgaagggc aggcgttggc gcaggttgag gtagtcggcg
     4201 cggttgtcga aaaaacctgc gatgatgccg gagcagaaca gccacagacc tgcaatggcg
     4261 gcataccaca gcgtcgggta agcgatgatg tctatggatt tgaactggta ggcggcggtg
     4321 tgggcatcga gtatgggcag ccggtacaga tgggcatagc cgaacgatat ggcgcacgcc
     4381 aaaaggatgg cgatggaaac gttgccgaag acggcgacac tttgggagcg gcatacgtcg
     4441 atgaggagct tggcgagttt gttgtccacc gctttgccgc cttcgttgag atcgacctgt
     4501 tcggcaaagc tggcggcagt catcgcgggc tgcttggtgg cgacggtgca gtgcagcata
     4561 tggatgatca taaagccgat gccgtagttg aacccagccg acaaggaagt gaggaagggg
     4621 ctgaggccga gtgagccgat gcggattttg agcagcgcca ttagggcgat gatgatgccg
     4681 ccgcctgccg ccgagtagaa cattttgaaa tattctttgc ggttgcgggt gatgtagtgt
     4741 tcgccgtggt ggcttttgtt ttcggtaatg ctgcgggcga gcatacggat gctgctgcgg
     4801 cgcaggtggc gggtgctgta ttgttcgacc gccgcgtaga tgagggagtt catcagcgcg
     4861 atggtcaggc ggttgctgtt gccggcgccg gtttggatgt cggtgagcag cttcagacgg
     4921 tctacggtct gccggagccg ttcgagcagg tgggcgactt tgaccgacga accggagccg
     4981 gcgcccgtcc ctttgcggcg caaatagtca atctggctga aacattggtc gaacatcact
     5041 tcgaggtggg cggtgtcgta aggcgtggtg ccgttgcggt agtgttcgac cagtttggcg
     5101 gtttcgcgtt ggagggcgac gaaggaagaa tcggcttcca gcaggcgcgg ggcgatgcgg
     5161 atgaggtcgg gttcgatggc ttcggatgcc gtccagacag acagcatttc gatggcgcgc
     5221 aaacgcgcat cggcaaggcg gatgcccgcc gtctgcaatt tggacggctc ggcgtggctc
     5281 cggatgagtt cgtataaaac cagccattgg cggatattga gcgtttgcag ccatttgtcg
     5341 tcgttttcgg aatggaaaag ataaaggaag acttcgccca agttggcaaa atctttatat
     5401 gacgggctga agcgttcgta gatgcgtatg cccatttccc gggcaaagct gtggcgcgag
     5461 aagatgccga gtttgatgag tgccggataa atgtgtattt gcgcgagcca aatgtaaaaa
     5521 caccggctga agcgggacag taattccctg tcttgtttga atgtgtcgat aatcaggtcg
     5581 aaacgttccc ccgcacattt tttgccgcca cggcgcaaaa atttaatcag tgcgttgagg
     5641 acgttgacaa aatcggtatg tcccaagctt tccgaaagca gggggcgcag gttttgaggg
     5701 gtaattttct tcatatcagc cattgtaccg catcggcaat ccggcgttaa acgccccgat
     5761 agcggggagg gcgcgcctgc ggtgggcggc gcattttccg ccggtttgaa atcaaactat
     5821 attaatgata taataaatat cgataaatca atctgataga ataatccgta ttgattttta
     5881 attttttcga ttataacaat ttcggccggt ttgtgcattt cgtcatccgg aagccggtat
     5941 tttaccggca ggttttgacc gctttgcccg atgaccgggt ttgtctgttt ttaacaaagg
     6001 agtgagaagg tttgtccctg tctgaattta tagaacgccg aacgtcattt aatccgatgg
     6061 ttattttgac gactttgttt tttgtgtgtg ttttggtggt attggtttta accgtgccgg
     6121 atcaggtgca gatgtggctc gatcgggcaa aagaagtcat ttttaccgag ttcagctggt
     6181 tttatgtttt aacgttttcc atttttctgg gtttcctgct gatactctcg gtcagcagtt
     6241 tgggaaacat caggctcgga cgggatgaag atgtgccgga attcggcttc ctgtcgtggc
     6301 tggcgatgct gtttgcggcc gggatgggcg tgggtctgat gtttttcggc gtggcagagc
     6361 cgttgatgca ttatttttcg gacattacgg ccggcacgcc ggaacacagg cagcagcagg
     6421 cattgctgca cacggtgttc cattggggcg ttcacgcttg gtcggtgtac ggtacgattg
     6481 cattggcttt ggcttatttc ggtttccgct acaagctgcc gcttgccctg cgttcttgtt
     6541 tttaccccct gttgaaagaa aaaatttccg gaaggttcgg cgatgccatt gatattatgg
     6601 cgttgcttgc tacttttttc ggcatcatca ccacattggg gttcggggct tcgcaactgg
     6661 gcgccggatt gcaggaaatg ggctggattg ccgaaaacag cttcagcgtg caggttttga
     6721 ttatcgccgc cgtcatgtcc ctcgccgtcg tttcggcaat atccggcgtg gggaagggcg
     6781 tgaaggtgtt gagcgagttg aacctgggcc ttgcgttttt gctgctgttt tttgttttgg
     6841 cggcgggacc cactgtttac ctgttgtcgg cattcggcga caacataggg aactacctcg
     6901 gaaatctggt gcgcctcagt tttaaaactt atgcgtacga acgggaacac aagccgtggt
     6961 ttgaatcttg gacggtgctt tattgggcgt ggtggtgttc ttgggcgccg tttgtgggtt
     7021 tgtttatcgc gcgcatttca aaggggcgca ccatccgcga gtttgtcttc ggggttttgc
     7081 tcatccccgg cctgttcggc gttttgtggt ttaccgtctt cggcaatacg gcgatttggc
     7141 tgaatgacgg ggttgcgggg ggaatgctcg aaaagatgac ctcctctccg gaaacgctgc
     7201 tttttaaatt ctttaattac ctccccctgc ccgaattgac gagcatcgtc agcctgctgg
     7261 tcatttctct gttttttgta acttctgccg attccgggat ttatgtcctg aacaatatta
     7321 cctctcggga caaaggcttg agcgcgccac ggtggcaggc ggttatgtgg ggcgtgctga
     7381 tgtctgccgt tgccgttttg ctgatgcgct cgggcgaact cggcaacctg cagtctatga
     7441 ccctgattgt ttccctgccg tttgccctgc tgatgctgat aatgtgtttc agcctgtgga
     7501 aaggcttgag tgcggataag aaatattttg agacccgggt taaccctacc agtgtatttt
     7561 ggacgggcgg caagtggaaa gaacggctgg tgcagataat gagccagacg caggagcagg
     7621 atattttaaa attcctcaaa cataccgcat cgcccgctat gcacgagttg caacgggagc
     7681 tttcggaaga atacggcttg agcgtccggg tcgataagat gtttcatcag gacgagcccg
     7741 caatcgagtt tgtcattcgc aaagagacga tgcgcgattt tatgtacggg attaagtctg
     7801 tcgggcagga tgtatccgat cagttgatta acgacggcaa cttgccgcat atccggcacc
     7861 agacaactta caaaccttac gcttattttt tcgacgggcg cgtcgggtac gatgtgcagt
     7921 atatgaacaa ggacgagctg attgccgaca ttttgaaaaa ctacgaacgt tatttgatgt
     7981 tgttggatga cgtcggtcag gaactgatgg cgcacgagca ggtggaattg gcagagtaag
     8041 tgccttatct ccggcgtttg ccaaaaaaaa tgcggtgaat cagattcacc gcattttttt
     8101 gccggacggg gcttcggacg gcacggcgtt catttgtttc cgtcaaactg ctccttcaac
     8161 acccgtttca ataccttgcc cgtagcgttg cgcggcagcc cgtctttaaa gtggatttgt
     8221 ttggggattt tgaaatttgc cagcacggta cgcaggtggc ggcggatttc gttctcgccc
     8281 aaatccatac cttccttcaa ttggacgaag gcgacgattt cctcgtcggc ataacggtct
     8341 ttcacgccga tgacggcagc ggcttcgacg gcatcgagtt tgtagatttc ttcttcgatt
     8401 tcgcgcggat agacgttttg acctttggaa ataatcaaat cttttttgcg gtcgacgata
     8461 aagataaagc cgtcttcgtc tatggtaacg aaatcgcccg ttttcaacca gccgttgacg
     8521 atggtttcat cggtggcggc aggcatattg aggtagcccc gcatcaccga accgcccctg
     8581 acgatcagtt cgcccacttc gccgcgcggc acttcgacca attcttcatc gacggctttg
     8641 gcttccaaac cgggcagggg gatgccgacg ctgcgggctt tttgcctctc gggcgtattg
     8701 acggcgacga cgggagaggc ttcgctcagt ccgtagcctt ccagcaattt ggcgcggggg
     8761 aacttggctt tgaaatcgag gatggtttgt tccgccaaag gcgcaccgcc gctgataaac
     8821 aggcgaatgc ggttgaacca tctgaaatac caagggattt tcgccttgct cattgcggtg
     8881 taaatcgcgg gtatgcccaa aaacacggtc gcgcgtttga gcagggcctg tttcaaaacg
     8941 ttggaaaacg ggaaaacgga tttgaccaaa ataatcgaac acgccatata aatcggcagc
     9001 agcaccatag ccgtcagcgt gaagctgtgg aacatcggca ggaaaacgat aaagcggtcg
     9061 cgcttggaga ttttaaagat gcgttcgatg ccgttcaggt tggcgaacag gttggcgtaa
     9121 ctgattagcg cgcctttggg atgccccgtc gtgccggagg tgtagatgat gtgtgccaaa
     9181 tcatttatcc ggggttggcg gcccaagtcg ggtttttcgg ggaagcggcg cacgtcttca
     9241 aaaaaggcat cgccttccgc catttcgcca gccggacggc ttttgtccgt ccaaatgatt
     9301 ttttcgacgg gcgtttgcgc cttcaagccc gccaactctt ttgacaggcc cgccgaggcg
     9361 aacaggaagc gcgccttgca gtcgttcagg atatacgcgt attcgctgtt tttcaaaaat
     9421 gtgttcatcg gtacggcgac cgcgccgatg gcggagacgg cgaaataggc ggtaataaat
     9481 tctgtggaat tggaaaccgc cagcgcgacc gtgtcgccga acttcacgcc gatattttgc
     9541 agatacgccg cgacggcttc ggcctcctgc ttgagcgcgc ggtaggcggt tttttccttg
     9601 ccgtcgaaca ctgccgtgcc gttgccgttt ttgcggcagg cggcggcgag catttcgtag
     9661 aaattggcat aagtccggtt catgaaactt cctttatggt tgcggacggc gaacgccctc
     9721 atttgaaaac aatatgccgt atgaaaacgg aaggcggcag tttatcatct ttgccttcag
     9781 acggcatatc aaacgccgcc tttgcgccgt atgccggagg ttcgcctgcg tctttccaat
     9841 gtctgaggcg aatgccgtct gaagcaatat gaaggtatgc gtttcagacg gcatccccct
     9901 gttgcggaaa taccgtccgc ctcctgtccg attattgcct gattgcggcg gcgcggacga
     9961 gttcttcgag gctgtcggcg agcatttccg ccgcgcgccg gtagcctttg gcggagaagt
    10021 gtacgccgtc tttggcggcc catccttggt tgagccagtt tttcatgctg catatgccgc
    10081 ccatggcgtt ttgccaagac cagaacatgg tttgtccctg ccgggcgacg cgccgctgca
    10141 tctgttggac ttcggtcagg cggacgggac gtgtgccgca tacgccgagc gtgtttttca
    10201 gggattcggg tgcgccgatg atgaggatgc cggcggcagg caggctgtcg tggatttggc
    10261 ggacggtatc cagccatttt tgttcagtgt cggcaatgtc gatgttgtcg ccgaaggctt
    10321 cgttggtgcc gtaggaaagg ataaccaaat cggcgccggt ttgggcaagg tcgttcatcc
    10381 ggtcggcacg ccatttcgac cactgggtta attgtgcgcc gttgatgccc atcgcggaaa
    10441 cggtaatgcc gccggcggga ttttcgatgt tgatgaagcc gatgtcccac ggcatttcgg
    10501 tctgtatggt caggggcagt gccgcgcccg tatccagtac ctgccagccg ccgccgttgg
    10561 cggagacggt gttgccgttg acggtcaggg tttgttcggc gagcaggggt ttggcaaaca
    10621 gggaaatgcg ctgtttgccg gtcttgccgt ccgatgcggt cagggtcatg ctgccgccgc
    10681 tgccggtgtg ggcgaggatg ccaccgagcg ggaagtctgc tgctgcactt cggctggtga
    10741 ggctttgcca gttgccgttg tgccggactg ccgccatgcg ctgccctttg acgttggcgg
    10801 ggtagaccca gcctatgccg ccgtcgcccc atgttttttg caggcgtttg cggaggctgt
    10861 cggtaaagaa gtcgccggcg gtgtgcgagt cgccgatttg caggatgcgg aaggtctcgc
    10921 cgctgccttg tgcgacggat cggagttttt tcatccaagg cgaggcggcg gcgttgccgt
    10981 agtcggtcaa gagcccgttt gtatcggtgt agggggcgga cggggaaacg gtaacggtgt
    11041 cgaggctgac ggaggcgacg ggcagagcgt ttgcctgcgt ggcggcgaac agggcggaaa
    11101 atgcgatgag gtgtttggga ttcatggctg tgtacttgat ggttgcgtac ttggttcaaa
    11161 aacgattttt tccattattt ttgtcgccag cagtttttgt ccttcggcgg taaagtgtat
    11221 gccgtccttg ctgcggtagc ggacgggttt gccgttgacg ttgacggagt ccgtgtagcg
    11281 gtcttccccg ccgctcaggg tgtgcgcggt gggaatcagg atgattttgc cttccaggtg
    11341 ttccgaaagc agtttgtcta ggtagcgcat ctgtccgtcg agcttggctt ttttcatata
    11401 ggggatgccg agccagacga cttggacgtg gtgcgcgcgg gcggcttcaa ggatgcggtc
    11461 gacgcgtttc aggtattctt gcgcccattc gtcggaagcg aatttgaggt atcgtttgcc
    11521 gacggggaaa tcccacgggt cgttcgggcc gaggaagacg gcgagcacgc tgatttcggg
    11581 gtgtttttgc agggtttctt caatcgtttt cggccagtcg aagaatttgg gataggaaag
    11641 ccccgtgctt tgtttgctga ggttgacgga ttcgatgccg tattgctgtt tcaggctttt
    11701 ttgcacgaag ggtgcaacgc cctgcatcag cgagtcgccg gcgaaaaaga ctttgtcgcc
    11761 gctgcggacg gcggcggctt cggtgtcttg tttccattct gtttcaccgg tttgagggac
    11821 ggcttcggat gcggcggctt cagacggcat atctgccgaa ccgccgtctt gagccgtcgg
    11881 cggcgtttcg ccggacagga aggctttgat gccgtctgaa agggcgtagg cgttttcttg
    11941 caacgccaca ccgctccgcc accatccgta ggcggcaagc ggttcgagcg gactgttgcg
    12001 gtggtaggtc tgctgccagt aggcgttgat ggggttttgg ctgaaccaca cggcaatcag
    12061 ggcagacatc agtatggagg cgaaaaggga aagaaagttt ttcatgtcca aacccttaaa
    12121 aattggcata aataaagccg ggtatccccg agggggcgag gacgataatc agcagcagga
    12181 cggcggaaac ggggataaac cacagccaca tcgggatttt ttccaaacct ttgaccgcgc
    12241 cgtcgaaagc gcgttgcagg taagggtaga gcagcatcac ggatgcaaac gaggcaagca
    12301 gcagcatgtc tgcccgttgc ggcgcgttcc agccgccggc attggcaaag agcgcgctga
    12361 aaaccgcgcc ggcatcgtcg ggattggcgg tattgaagac gacaaagcta aggcagacga
    12421 aatggaaggt aatgagccat gagagcggcg caaggtattt cagacggcat agtgcgttgc
    12481 gtccgaaata gcggtcgccc gtgttgagca gcaccagcgc cgtgccgtgt agtgcgcccc
    12541 aaatgaggaa gttccagccg tagccgtgcc agatgcctga gagcaccatt gccgccatca
    12601 ggttgaactg tgtccgcaaa aagccttttt tgctgccgcc caaggggatg tagatgtagt
    12661 cgcgtatcca ggtggaaagg ctgatgtgcc atttgtccca aaatgcgcgg atattcgcgg
    12721 cacgaagcgg tgcggagaag tttttgggca gccggaagcc cagcagcatt gccattccga
    12781 taaccaagtc ggaatatccg gaaaagtcta aaaagagttg gaaggtatag ccgtacacgc
    12841 ccgccaatac gccccagccg tcgaattggg cgggattttc aaatacgggc gacacccagt
    12901 tttccgccag catccccgcc agccaccatt ttttggcaat acccagcaga atcagggaaa
    12961 cggcgagtgc ggggcggacg ggcgaacgcg gtcggtgggt acggatttgc gccaatgcgc
    13021 ctgcctgctc gccgtctgcg cttttgaatg cggcggcgcg gataatcggg ccggaggtaa
    13081 cggtggggaa aaaactcagg tgcagtagca gctcgtgcca cccgaaacgc gcggcgtgcg
    13141 gggcgcggaa gcagtaaaca agataggcga gcgactggaa ggtgtaatac gaaagcccca
    13201 gcggcatcag gatgtcgatt gcgccgcctt ttccggcata ttgggcaatc agcggccgga
    13261 aaaagtcgaa atatttgaaa aagcccaaga cggtcagcga ggcggcaatg ccgcacccca
    13321 gccaaaagcg gcgcgtacct tcgcgatcgg aacggagcag ttcgcccaaa aggtacacgc
    13381 aggaggaata aaggacgatg attgccgcaa atacggggcc gatatggtag agccagccca
    13441 taccggcagc caaaagcagc aggttttgga cggacgggta tttcgccaag ccccagtaaa
    13501 tcggcaggaa ggcgagaaag aacagtgcga actcgacaga cagcagcggc ataaaccatc
    13561 cttggaacaa aaaataggcg ttaaaacacg gcaaagcccg ttattttaag caggggtgga
    13621 tatttcttca atcaaaatgc cgtctgaagg gcgggagggg cttcagacgg catcgtgcgg
    13681 tcaggttcgg atatagcggt tcaggttcag gcagacccac aaacctgccg ccgccgtcag
    13741 catacccgca acgccgatgc aggaaatgcc cgagtattgc gtaacccaat gccccagcag
    13801 cgcgccgccg ccgataccga cgtt
//
LOCUS       DAWHRS010000045        13626 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWHRS010000045 DAWHRS010000000
VERSION     DAWHRS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13626
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.045"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(23..715)
                     /locus_tag="WIB82_000910"
     CDS             complement(23..715)
                     /locus_tag="WIB82_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ3842229.1"
                     /translation="MFHAFIPRIHIMIKNLYKLINYAALIGFAPNIVFAWPMFVDPPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1021..1578)
                     /locus_tag="WIB82_000911"
     CDS             complement(1021..1578)
                     /locus_tag="WIB82_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842230.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWGYHGANLYNHGKLGTAQGAATATGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1822..1980)
                     /locus_tag="WIB82_000912"
     CDS             complement(1822..1980)
                     /locus_tag="WIB82_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842231.1"
                     /translation="MKIPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(1977..2819)
                     /locus_tag="WIB82_000913"
     CDS             complement(1977..2819)
                     /locus_tag="WIB82_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3842232.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3058..3978
                     /gene="pyrB"
                     /locus_tag="WIB82_000914"
     CDS             3058..3978
                     /gene="pyrB"
                     /locus_tag="WIB82_000914"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ3842233.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWNCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            3988..4446
                     /locus_tag="WIB82_000915"
     CDS             3988..4446
                     /locus_tag="WIB82_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ3842234.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4511..5176
                     /locus_tag="WIB82_000916"
     CDS             4511..5176
                     /locus_tag="WIB82_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842235.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQILAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5239..6456)
                     /locus_tag="WIB82_000917"
     CDS             complement(5239..6456)
                     /locus_tag="WIB82_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ3842236.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6598..7143
                     /gene="def"
                     /locus_tag="WIB82_000918"
     CDS             6598..7143
                     /gene="def"
                     /locus_tag="WIB82_000918"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ3842237.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7230..8156
                     /locus_tag="WIB82_000919"
     CDS             7230..8156
                     /locus_tag="WIB82_000919"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ3842238.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSKS
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGAKL"
     gene            8235..9494
                     /gene="rsmB"
                     /locus_tag="WIB82_000920"
     CDS             8235..9494
                     /gene="rsmB"
                     /locus_tag="WIB82_000920"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ3842239.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLMAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLVASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASTACADAQDLSAWYDGKPFDAVLADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDEQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9460..10059
                     /locus_tag="WIB82_000921"
     CDS             9460..10059
                     /locus_tag="WIB82_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ3842240.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10059..12179
                     /locus_tag="WIB82_000922"
     CDS             10059..12179
                     /locus_tag="WIB82_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ3842241.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGRLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSSSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            12172..13449
                     /locus_tag="WIB82_000923"
     CDS             12172..13449
                     /locus_tag="WIB82_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ3842242.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSTVENALKYGAAQTETGPVFDKLGNSAAIQEMNCEVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
BASE COUNT         3216 a         3462 c         3894 g         3054 t
ORIGIN      
        1 taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt tctaccgtaa
       61 aacgtgtttg acgaacggga ttcttattaa aaaacatctg atttctaaca aaatcagtat
      121 tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt ttccatgcgc
      181 tcaagaattg tgatttgctc attgagacgt gccccagcga tggatcagcc agcaaaacag
      241 tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat tttaaataca
      301 caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa ccttgtgctt
      361 caaaacccag ttcgggcatt atgcgttgca tatcgtcaaa agaagcacgc atttgggttt
      421 tatccatttt gtctaagatt tccgcttcag aataatgtct gccataaaaa ttattcagta
      481 acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact atgccggaat
      541 ctcgccgtgc tttccaactc cgtacatgga tgttttggta agaagcgggg gggtcaacaa
      601 acataggcca agcaaaaact atatttgggg cgaaaccaat caaagccgca taatttatca
      661 atttataaag attttttatc ataatatgta tacgcggaat aaacgcatga aacataaaaa
      721 aataaaatca tatgcaattt tattgcatga ataaatatga ataaaataga taatgatggg
      781 agtaaatacg ccatgtattt tggaagttta aatttattaa taataaaata atttatcgta
      841 gcgcaaataa atcccaaaat tggaacgatt agaaaaaaaa ttaatacacc catcatcatg
      901 caaactctat attaataaat agctaactaa ttctattgtg gaaattagtt agctaactaa
      961 aagttattaa tgattatttt cgagaattga ctgcattgtt ggcagcattg gcaccaaaac
     1021 ctagtgcatg aatacccggt ctccatgcca aattcccagc caatccgcct ccggcagcag
     1081 cagccagccc tgttttgccg ctactcctgt tgccgcaccg attcctgtcg cagtagccgc
     1141 gccttgcgca gttcctaatt taccatgatt atacaaatta gcaccatgat acccccatgc
     1201 acctaatgca ccgccaaaag cagcggctgc aataatggga acaaattcac cttgtgtttc
     1261 tttcatttca gcctgtgata attgaattgc tttcacattt tggctgtcaa aaacttggct
     1321 gtctaaattt tgcgccatta caggtgtaat catcatagcc attacagttg caattttcgt
     1381 tgcgctggtt tgcacataaa taggattagc aaattcgctt tgattgcgtt cagtgttgat
     1441 gtagctaata ctgctttcta gtttgaattt acccttgtca gtcaataatt cttccaaact
     1501 taaaggtaag tcagcataag caatttggct ggcaacagtc aaaataaaat ctattagaca
     1561 tttgtgtttt tgcatcattt cgtttgattt tctaggtttt gagaatgata caaagttttt
     1621 tacaaagtaa agagtcactc tgaaaaaact tttttcatta taaatcaaaa tattgataga
     1681 ataaatagcg agcatcgatt cacggtgcgc tttagtgcaa agcgtacaac gcgagcctga
     1741 accaccagcc gcaacaggaa aagaaagccg atagagtgca atgcttgcca acgtgcaagc
     1801 gagcttgcaa gaacgcttgg ctcaacgaga gcaggcaaga cagaaagcag aaagcaggat
     1861 aggagcggta acgcaaaggt ctcgggcttt gatttcgccg taaaccctgc tgccgccttg
     1921 tccggaaagg gtgcaggcgg cgagtgccga cagggtgcag atggggaggg ggattttcat
     1981 ttggggtcgc aacggaagtg gtatgcgcag atttcaaaac cgtttttgaa atacaggcgg
     2041 tgcgcgtcgg cacggtcgtg gttgacgtgg acgttgaggt ggattttggt tacccctgtt
     2101 tccgcgccga ttttgcggac ttcttccaaa aggcgcgagg cgtagccttt gcggcggctt
     2161 tgcggcaggg taacgatgtc atcgatgtgg atgtggcggc cgctggcgag ggtgcaggct
     2221 tcgcggaagc cgcagacggc gacggcattg tgtttgcctt cttcaaaaat acccagcagg
     2281 cggtagcctt gggggcgttg gactttgttg atctgttcgg taaagcggtt gatgtcggtc
     2341 agggcggaac gcaaaacgct caaggctgca aaggcggtgg cggtgtcgtc cgcgccgatt
     2401 tcgcgcaaaa cgtaggatgc gcccgaggcg gtctgttcct gtgctttctc ggcggcgtgt
     2461 ttttcttcga ttgcctgtgc cagcatgacg tgttcgtcgg cagggttgtt ttgtccgccc
     2521 tgttcgcgtt cttcgagcag ggctttgcag tcgatgacgc gcaggtcgtt gtcggcggca
     2581 aagtccatca ggaagcggaa catttgggga ttgtcggttt ccagtttttt atcgacggcg
     2641 acgcagcgga tgttgtccac cagtatgggg cgcacccatt tcgaatagga aagcctgtgg
     2701 tctttggtga acgaggacag aatgccgcac aggcgttccg cccagtcgct gggacggaaa
     2761 atcttgccgg aactcgttgt gccgtggatg acgacttcgt aggggttgca gactaacatg
     2821 gcggcttcct gaaaagaaat gtctagcgcg attatacctt atgcttatgc gggcgtgttt
     2881 ggatatgccg tctgaaaagt acgggattcg tgcggtaaaa ctttgcggcg gcaaatgtgc
     2941 gataatacgc gccgtattgc cgcttttgcg aagctgttcc gcaaacatac gggcggcgtg
     3001 gacgacgtat aaccggatac ccgcctgacg cgggtttttt acggaagggg ggcaaaaatg
     3061 cctaatccgc tttacagaca gcatatcatc tccatttcgg atttgtcgcg cgaacagttg
     3121 gaatgcctgc ttcagacggc attgaagctg aaggcgcatc cgcgcggcga cctgttggaa
     3181 ggcaaactta tcggttcgtg ctttttcgag ccgtccacgc gcacgaggct gtcgtttgaa
     3241 acggcggtgc agcgtttggg cggcaaggtc atcggtttct cggacggcgc gaataccagt
     3301 gccaaaaaag gcgagacgct tgccgatacc gcccgcatca tttccggata tactgatgct
     3361 atcatccaac gccaccccaa agacggcgcg gcgcgcgtgg cagcggagtt ttcgcgcgtc
     3421 cccgttatca acgccggcga cggcacgaac cagcacccca gtcagacgct gctcgacctg
     3481 gttaccattt atgaaacaca gggacgtttg gacaagctca aaatcgccat ggcgggcgac
     3541 ttgaaatacg gacgtaccgt gcattcgctt tgtcaggcgt tgaaacgctg gaattgtgaa
     3601 tttgcctttg tttcgccgcc cagcctagcc atgcccgact atattaccga agagttggac
     3661 gaagccggct gccgataccg tatcctcggt agtttggaag aagcggcgga atgggcggat
     3721 atcctgtata tgacccgcgt ccagcgcgaa cgtttcgacg aacaggaatt tgccaaaatc
     3781 caaggcaaat tcaacctcga agcgtctatg ctcgcccgcg ccaaaccgaa cctgcgcgtg
     3841 ctgcaccccc tgccgcgcgt ggacgaaatc catcccgatg tcgatgccac gccgcacgcc
     3901 tattatttcg agcaggcgac caacggcgtt tatgcgcgta tggcgatatt gtcgctggtg
     3961 ttgaacgaag aagtgtgagg aaccgatatg gaaaccccga aactcagtgt cgaagccatt
     4021 gaaaaaggta cggttatcga ccatattccc gccggcaggg ggctgaccat cctgcgccag
     4081 ttcaaacttt tgcactacgg caacgcggta accgtgggct tcaacctgcc cagcaaaacc
     4141 caaggcagca aagacatcat caaaatcaaa ggcgtgtgct tggacgacaa agccgccgac
     4201 cgcctcgccc tgttcgcccc cgaagcggtg gtcaacacca tcgacaattt caaggtcgtg
     4261 cagaagcggc atttgaacct gcccgacgaa atcgccgaag tgttccgctg tccgaacacg
     4321 aattgcgccg gccacggcga gccggtcaaa agccggtttt atgttaaaaa gcacaacggg
     4381 cagacgcggc tgaaatgcca ctactgcgaa aaaacctaca gccgggattc ggtggcggaa
     4441 gcctgacgga ttcccttaaa ccgagtgggc ggcatttcgt ctgccgcctg ttttgccaat
     4501 ctgaaatgga atgatgatgc acgcttctgt ccaaagccgt ttcgcaccga tactttatgt
     4561 tttgattttc tttgccggtt ttttgaccgc gcaaatctgg ttcaatcaga aagcctatac
     4621 tgaagagctg cctccgcttc tgtccgcatt gtccgccgtc gcgctggtgt ggctggcgtg
     4681 ggcgttcgtg tcggcgcgtt caaaggccaa ggcggaaaag ttctaccgcg aaaaaatgat
     4741 acagaacgaa agcatacacc ccgtcctgca cgcctctttg caacacttgg aacacaagcc
     4801 gcaaatactc gccctgctgg tcaaaaacca cggcaaaggg atggcggaac aggtcaggtt
     4861 caaggcggaa gtgctgcccg acgacgaaga cgcgcgcacg attgccgccg agttggcaaa
     4921 aatggatatg ttcgcattgg ggacggacgc ggtcgcctcg ggcgaaacct atggacgcgt
     4981 gttcgccgat attttcgagt tgtcggcggc tttggaaggg cgcgcgttca aaggaatgtt
     5041 gaaactgacg gcggaatata aaaacatctt cggcgatgcc tgccgttcgg aaacggcgtt
     5101 ggagttgggc gcactcaatc aggcgttgca ggagatttca aaaacatcgg aaaagtccaa
     5161 acggatattt tattgaagat ggaaaaatgc cgtctgaaac ggaaggtgtt tcagacggca
     5221 tttttgtcgg atgattaatt attcggagcg gttgaagcca aacttcacgc ggctgcggcc
     5281 ctgatccggt atattgtcca aatcgcgtcc cggattggcg gcggtgtcgc ctacggaaat
     5341 atcggagatg ttttccaaaa tgatggcgga cgacaggtgt tcggaggtgc ggtaaaccat
     5401 tgccaagccc acttcttcgg caggagtgga aatcagctcg acggtatccc tgcttttgaa
     5461 attgttggag aggtcgacct gcatcgtttt cttgcgtttg tagaggctca aaaccgtgcc
     5521 tttgtccaaa ccgtccgcct cgcctttgtc gatggtgatg gtttgaaact ggccggcaat
     5581 ccttgtgcct tcaaacacgg aaacgatttt agcctgaacc gggcgggacg gttcgtgcgg
     5641 catcatgttg aagcggtcgg tgtcttccgg cattttcatc aggtagtcgc cctgctgtat
     5701 ttcggaaatg gcggtttcga ccaccagcgg ctgtatcgaa ggggtgcgca gcggggtaat
     5761 caaaggatgg gtgcgggtat ggtattcgtt gtctttcggc cgttctccag cctgtttcga
     5821 gcgttgttcg aggacagagt cggtatagtc gagggagcgc acgatgccgc tgaatgcgac
     5881 ttcctgcccg aggaatttac ccgtatccgg atcggtgatg tttttattga ttcggtaggt
     5941 caggtagcgg cccggctctt tcaggccttt ggtgtaaacc ctggtgcctt tggtgtacag
     6001 cagcctgcct tccgggcccg agagcaggcg cggcgcggca gcggtttctt tgcgggaaac
     6061 gatttgcgga tgccgcataa agatgcggta gaagttgaca tcgatggcgg gaataccgta
     6121 tccggacact tccttatccg gactcatttt gacgacgggg atgccgtctg tctgttccaa
     6181 gccgaggcgc ggttcgccgt caacgtggcg caacaccaat acctggtccg gataaatcag
     6241 gtcgggattg tggatttgat cccggttcgc gtcccacagg cggccccatt gccacgggct
     6301 gtacaggtat ttgcccgaaa tgccccacag ggtgtcgccc tgtttgaccg tgtagcgttc
     6361 cggcgcgttc gggcgcacct ccaaatttgc cgccaaagtt tgtgttgaga atgccatacc
     6421 tgccgcgcag agcagggtta taatacgacg ttgcataacc gttcccctta tctgataaat
     6481 ttcggtttgt cttgcttgat tgggttggaa aaagcggcgg cagcccctcg ggatgtgccg
     6541 cgtgataaaa aatgttccgc attttaacat cgaattatcc gcaccatcac ggtaattatg
     6601 aaaaacaggc ggcgtatccg ccgaaggaaa gagaaaatta tggctttatt gaatatcttg
     6661 caatatcccg acgagcgtct gcacacggtg gcaaagcctg tcgaacaagt cgacgagcgc
     6721 atccggaagc tgattgccga tatgtttgaa acgatgtacg aatcgcgcgg catcgggctg
     6781 gcggcgacgc aggtcgatgt gcacgagcgc gtggtcgtga tggatttgac cgaagaccgc
     6841 agcgaaccgc gcgtgttcat caaccccgtc atcgttgaaa aagacggcga aaccacttac
     6901 gaagagggct gcctgtccgt gccgggcatt tacgacaccg taacccgcgc cgaacgcgtc
     6961 aaggtcgagg ctttgaacga aaaaggcgaa aagttcacgc tggaggcgga cggcttgttg
     7021 gcgatttgcg tgcagcacga gttggaccac ctgatgggca tcgtgtttgt cgaacgcctt
     7081 tcccaactca agcaggggcg gattaagacc aagctgaaaa aacgtcagaa acatacgatt
     7141 tgaccctttt gccgtgccgt ctgaacgctg caaagttttc agacggcacg gtcttgtccg
     7201 acaattttac gcacgcgcag gaacacgcta tgaaagtcat cttcgccggc acgcccgatt
     7261 ttgccgccgc cgccttaaga gccgttgccg ccgccggttt tgaaattccg ctggtgctga
     7321 cccagcccga ccgtccgaaa gggcgcggta tgcaactgac tgccccgccc gtcaaacaag
     7381 ccgcgctgga actcggtttg cgcgtcgaac agcccgaaaa gctgcgcaac aacgccgaag
     7441 ccctgcaaat gctcaaagag gtcgaggcag acgtaatggt ggttgccgcc tacggtttga
     7501 ttctgccgca ggaagtgttg gatacgccga aacacggctg cctcaacatc cacgcttcgc
     7561 tgttaccccg ttggcgtggc gcggcgccga ttcaacgcgc gattgaagcc ggcgatgccg
     7621 agacaggcgt gtgtattatg cagatggaca tcggtttgga caccggcgat gtggtcagcg
     7681 aacaccgcta cgccatccaa ccgaccgata ccgccaacga agtccacgac gcgctgatgg
     7741 aaatcggtgc ggcggcggtt gttgccgatt tgcaacagct tcaaagcaaa ggccgtctga
     7801 acgcggtcaa acagcccgaa gaaggtgtta cttacgcgca aaaattgagc aaagaagagg
     7861 cgcgtatcga ttggagcaaa agcgcggcgg ttatcgaacg caaaatccgc gccttcaacc
     7921 ccgtgcctgc cgcgtgggtt gagtatcagg gcaagccgat gaaaatccgg cgggcggaag
     7981 tggtggcgca acaaggcgcg gcaggcgaag tgttgtcctg ttcggcggac ggtttggtcg
     8041 ttgcctgcgg cgaaaacgcg ctgaagatta ccgaattgca gcctgccggc ggcaggcgga
     8101 tgaatatcgc ggcgtttgca gcaggacggc atatcgaagc aggggcgaag ctgtaaatcc
     8161 cttcagacgg cattccgatc cgcaaacggg aatgccgtct gaaaccatca gtcgaagaaa
     8221 gcgaatcaca taatatgagt atggcacttg cccaaaaact tgccgccgac agcattgcgg
     8281 cggttgccga aggacgtaac cttcaggacg tgttggcgca aatccgcacc gcgcatcccg
     8341 accttatggc gcaggaaaac ggcgcgttgc aggacatcgc ctacggctgc cagcgttatt
     8401 tgggcagttt gaaacatatg ctcgcgcaga tgctgaaaaa gccgattggc aatccgcagc
     8461 tcgaaagcct gcttttggcg gcgttgtacc agctgcatta cacgcgcaac gcgccccacg
     8521 ccgtggtcaa tgaggcggtg gaaagcatcg cgaaaatcgg acgcgggcag taccgttcgt
     8581 ttgccaacgc ggttttgcgc cgctttttgc gcgaacgcga caagcttgtg gcttcctgta
     8641 aaaaagacga tgtagcgaaa cacaacctgc cgctgtggtg ggtggcttac ttgaaaaacc
     8701 attatccgaa acactggcac aacatcgccg ccgcgctgca atcccatccg ccgatgactt
     8761 tgcgcgtcaa ccgccgacac ggcaatgccg aaagctattt ggaaaaactg gtggcggaag
     8821 gtatcgcggc taaggcgttg gacgaatatg cggttacgtt ggaagaagcc gtgccggtga
     8881 accgcctgcc tggtttttca gacggcattg tttcggtaca ggacttcggc gcgcagcagg
     8941 cggcgtattt gttaaacccg aaagacggcg aacggatttt ggacgcgtgc gccgcgccgg
     9001 gcggcaagac ggggcatatc ttggaactgg cggattgccg tgttaccgcc ttggacattg
     9061 atgcaggccg tctgaaacgg gtggaagaca atatcgcgcg tctgggcttt cagacggcat
     9121 cgacggcgtg tgccgatgca caggacctgt cggcatggta tgatgggaaa ccgtttgatg
     9181 ccgtccttgc cgacgtgccg tgtaccgctt ccggcgtggc gcggcgcaat cccgacgtga
     9241 aatggctgcg ccgtccgacc gacgcgctca aaaccgcccg ccagcaggaa gccctgctgg
     9301 acgcactttg gcagacgctg aaacagggcg gcagaatgct gcttgccacc tgttccgtgt
     9361 tcgtcgaaga aaacgacgag caattgcaaa aattcctcaa ccgccatgcc gatgccgaac
     9421 cgatcgaatc gcgggtgctt ttaccgaaca aacaccaaga tggcttttat tacgcgctta
     9481 ttcaaaagca ttaaacaatg gcttgtgctg ctgccgatgc tttccgtttt gccggacgcg
     9541 gcggcggagg ggatagatgt gagccgcgcc gaagcgagga taaccgacgg cgggcagctt
     9601 tccatcagca gccgcttcca aaccgagctg cccgaccagc tccaacaggc gttgcgccgg
     9661 ggcgtgccgc tcaactttac cttaagctgg cagctttccg ccccgataat cgcttcttat
     9721 cggtttaaat tggggcaact gattggcgat gacgacaata ttgactacaa actgagtttc
     9781 catccgctga ccaaccgcta ccgcgttacc gtcggcgcgt tttcgacaga ctacgacacc
     9841 ttggatgcgg cattgcgcgc gaccggcgcg gttgccaact ggaaagtcct gaacaaaggc
     9901 gcgctgtccg gtgcggaagc aggggaaacc aaggcggaaa tccgcctgac gctgtccact
     9961 tcaaaactgc ccaagccttt tcaaatcaat gcattgactt ctcaaaactg gcatttggat
    10021 tcgggttgga aacctctaaa catcatcggg aacaaataat gcgccgtttt ctaccgatcg
    10081 cagccatatg cgccgtcgtc ctgttgtacg gactgacggc ggcaaccggc agcaccagtt
    10141 cgctggcgga ttatttctgg tggatagttg cgttcagcgc aatgctgctg ctggtgttgt
    10201 ccgccgtttt ggcacgttat gtcatattgc tgttgaaaga caggcgcgac ggcgtattcg
    10261 gttcgcagat tgccaaacgc ctttctggga tgtttacgct ggttgccgta ctgcccggcg
    10321 tgtttctgtt cggcgtttcc gcacagttca tcaacggtac gattaattcg tggttcggca
    10381 acgataccca cgaggcgctt gaacgcagcc tcaatttgag caagtccgca ttgaatctgg
    10441 cggcagacaa cgcccttggc aacgccatcc ccgtgcagat agacctcatc ggcgcggctt
    10501 ccctgcccgg ggatatgggc agggtgctgg aacattacgc cggcagcggt tttgcccagc
    10561 ttgccctgta caatgccgca agcggcaaaa tcgaaaaaag catcaacccg cacaagctcg
    10621 atcagccgtt tccaggtaag gcgcgttggg aaaaaatcca acgggcgggt tcggtcaggg
    10681 atttggaaag cataggcggc gtattgtacg cgcagggctg gctgtcggcg ggtacgcaca
    10741 acgggcgcga ttacgccttg tttttccgcc agccgattcc caaaggcgtg gcagaggatg
    10801 ccgtcttaat cgaaaaggca agggcgaaat atgctgagtt gagttacagc aaaaaaggtt
    10861 tgcagacctt tttcctggca accctgctga ttgcctcgct gctgtcgatt tttcttgcac
    10921 tggtcatggc actgtatttc gcccgccgtt tcgtcgaacc cgtcctatcg cttgccgagg
    10981 gggcaaaggc ggtggcgcaa ggcgatttca gccagacgcg ccccgtgttg cgcaacgacg
    11041 agttcggacg cttgaccaag ttgttcaacc acatgaccga gcagctttcc atcgccaaag
    11101 aagcagacga gcgcaacagg aggcgcgaag aagccgccag acattatctt gaatgcgtgt
    11161 tggaggggct gaccacgggc gtggtggtgt ttgacgaaca aggccgtctg aaaaccttca
    11221 acaaagcggc ggaacagatt ttggggatgc cgcttacccc cctgtggggc agcagccggc
    11281 acggttggca cggcgtttcg gcgcagcagt ccctgcttgc cgaagtgttt gccgccatcg
    11341 gcgcggcggc aggtacggac aaaccggtcc atgtgaaata tgccgcgccg gacgatgcca
    11401 aaatcctgct gggcaaggca accgtcctgc ccgaagacaa cggcaacggc gtggtaatgg
    11461 tgattgacga catcaccgtt ttgatacacg cgcaaaaaga agccgcgtgg ggcgaagtgg
    11521 ccaaacggct ggcacacgaa atccgcaatc cgctcacgcc catccagctt tccgccgaac
    11581 ggctggcgtg gaaattgggc gggaagctgg atgagcagga tgcgcaaatc ctgacgcgtt
    11641 cgaccgacac catcgtcaaa caggtggcgg cattgaagga aatggtcgaa gcattccgca
    11701 attatgcgcg ttcctcttcg ctcaaattgg aaaatcagga tttgaacgcc ttaatcggcg
    11761 atgtgttggc attgtatgaa gccggtccgt gccggtttgc ggcggagctt gccggcgaac
    11821 cgctgatggt ggcggcggat acgaccgcca tgcggcaggt gctgcacaat attttcaaaa
    11881 atgccgccga agcggcggaa gaagccgatg tacccgaagt cagggtaaaa tcggaagcgg
    11941 ggcgggacgg tcggattgtc ctgacggttt gcgacaacgg caaagggttc ggcagggaaa
    12001 tgctgcacaa cgccttcgag ccgtatgtaa cggacaaacc ggcgggaacg ggattgggtc
    12061 tgcctgtggt gaaaaaaatc attgaagaac acggcggccg catcagcctg agcaatcagg
    12121 atgcgggcgg cgcgtgtgtc agaatcatct tgccaaaaac ggtagaaact tatgcgtagc
    12181 agcgatattt taattgtaga cgacgaaatc ggcatccgcg acctgctgtc ggaaatcctg
    12241 caggacgaag gttattcggt cgcattggcg gaaaacgccg aagaggcgcg caagctgcgc
    12301 catcaggcgc gccccgcgat ggtgctgctg gatatttgga tgcctgattg cgacggcatc
    12361 acccttttga aggagtgggc gaaaaacggg cagctcaata tgccggtggt gatgatgagc
    12421 gggcatgcca gcatcgacac agcagtggaa gccaccaaaa tcggcgcgct cgactttttg
    12481 gaaaaaccga tttccctgca aaagctgctg tccacggttg aaaacgcgtt gaagtacggt
    12541 gcggcgcaaa ccgaaacggg gcctgtattc gacaagctgg gcaacagtgc cgcgattcag
    12601 gagatgaact gcgaagtcga agcggcggca aagcgcacgt cccctgtatt tttgacgggc
    12661 gaagcgggtt cgccgtttga aaccgtggcg cgctatttcc ataaaaacgg tacgccgtgg
    12721 gtcagtccgg caagggtcga atatctgatc gatatgccga tggaactgtt gcagaaggcg
    12781 gagggcggcg ttttgtatgt cggcgacatc gcccagtaca gccgcaacat ccaagccggt
    12841 attgccttta ttgtcggaaa ggcggaacac cgccgcgtca gggtggtcgc atcgggcagc
    12901 agggcggcag gttcagacgg cattgcctgc gaggaaaagc tggcggaact gctgtcggaa
    12961 tcggtcgtcc gtattccgcc gctgcgtatg cagcatgaag acattccctt cctgatacag
    13021 gggattgcct gcaatgtggc ggaaagccaa aagattgcgc ctgcctcatt cagtgaagag
    13081 gcacttgccg cattgacccg ttacgactgg ccgggaaatt tcgaccaact gcaaagcgtc
    13141 gttgcaacgc tgttgttgga ggcggacgga caggaaatcg gcgcaggggc ggtttcttcc
    13201 cttttggggc agaatgtgcc tgccgaggga gcggaagata tggtgggcgg gtttaatttc
    13261 aacctgcccc tgcgcgaatt gagggaggag gtggagcggc gttatttcga gtaccacatc
    13321 gcccaagaag gtcagaatat gagcaaagtg gcgcagaaag tcggtttgga acgcacgcac
    13381 ctttaccgca aactcaaaca gctcggcatc ggcgtttcgc gccgggcggg ggaaaaaacc
    13441 gaagaatagg cccggacggc cggttttccg gctgcgggct tttgttttca gacggcattt
    13501 ggtgcaaatg ccgtctgaaa tcgtaagggg acggatttta tgacagagga cgaacgtttc
    13561 gcgtggttgc aactggcttt tacgccctat atcggtgcgg aaagtttctt gctgctgctg
    13621 caccat
//
LOCUS       DAWHRS010000046        13389 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWHRS010000046 DAWHRS010000000
VERSION     DAWHRS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.046"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(111..539)
                     /locus_tag="WIB82_000924"
     CDS             complement(111..539)
                     /locus_tag="WIB82_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ3842243.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(604..2352)
                     /locus_tag="WIB82_000925"
     CDS             complement(604..2352)
                     /locus_tag="WIB82_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ3842244.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2679..4112
                     /gene="ccoN"
                     /locus_tag="WIB82_000926"
     CDS             2679..4112
                     /gene="ccoN"
                     /locus_tag="WIB82_000926"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ3842245.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4139..4750
                     /gene="ccoO"
                     /locus_tag="WIB82_000927"
     CDS             4139..4750
                     /gene="ccoO"
                     /locus_tag="WIB82_000927"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ3842246.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4755..4925
                     /locus_tag="WIB82_000928"
     CDS             4755..4925
                     /locus_tag="WIB82_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ3842247.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4949..6046
                     /gene="ccoP"
                     /locus_tag="WIB82_000929"
     CDS             4949..6046
                     /gene="ccoP"
                     /locus_tag="WIB82_000929"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ3842248.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAVSEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6086..6280
                     /locus_tag="WIB82_000930"
     CDS             6086..6280
                     /locus_tag="WIB82_000930"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ3842249.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6482..7891
                     /locus_tag="WIB82_000931"
     CDS             6482..7891
                     /locus_tag="WIB82_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ3842250.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7993..11202
                     /gene="recC"
                     /locus_tag="WIB82_000932"
     CDS             7993..11202
                     /gene="recC"
                     /locus_tag="WIB82_000932"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ3842251.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LDDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSEMAGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPKGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLTDIVRIWRQPATVGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTFAAAEKKEEAGAA"
     gene            complement(11272..12840)
                     /gene="mtrF"
                     /locus_tag="WIB82_000933"
     CDS             complement(11272..12840)
                     /gene="mtrF"
                     /locus_tag="WIB82_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ3842252.1"
                     /translation="MNQTDTRRGGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIYVVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            13172..13384
                     /locus_tag="WIB82_000934"
     CDS             13172..13384
                     /locus_tag="WIB82_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842253.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3349 a         3233 c         3557 g         3250 t
ORIGIN      
        1 cactgtaaaa acggagaagg acagaggacg gcagtaaggt ttgccgtcct ctttttcaag
       61 ttgcggtgga atgccgcttg aaggtgtggt ttaaacgttt cggcttgtaa tcagacgcgg
      121 gcgttgtcca gccgctctct gaagttttcc aaatttacgc ctgccgcttg ggcgcggcgg
      181 atgatttcgt caaccggcat accttctgcc gcccggcgga agccccacag gagggagcag
      241 cgcgtaccgg tccggcaata ggcaaggacg ggatattcgg cttgtccgat gagttggcgg
      301 aaggtttcga catcgtgttt ttggatgtcg cgtgcggtaa cgggttggtg atggaatcca
      361 gtaacgcctg cttgttccag ccactgtttg atttgggcga agtcgggttg cgattcttct
      421 tcgcggtcgg ggcggttgca gatgacggtt ttgatgccca gttgcgcgat ttgttccgca
      481 tcggctttgg tcagttgcgg agaaatatag aggtgttcgt caagtttcag aatggccatt
      541 ttgtttcctt tactttgcgg ttgtttttca gacggcattt tgtgtgcaat gccgtctgaa
      601 agtttaaccg aacaaccagt tgtccaacag gttccggtct gtgatatgta ggatggcagt
      661 atccgctgcc gctgcctgaa ccgtcaagac ggtttgacgc aatatgccgg cacggaagaa
      721 gtggatattg atttttgcat tgacgtggta tcgggcccat tgtgcggtaa agtcggtgca
      781 ggcataaccg tctaaagcaa tgattttgtc ttgcgggcac agtgccgcag attccgcgct
      841 gccgccgttg aagacatggg tcaggacgat gtggtcggtg ttttgtttga aacgtgcgcc
      901 aaaatcgcct gccgacggga cggggcagat gtgttctgcg tatccgccgc cgtgttgtcg
      961 gggaagcggc aggaaggtca gtcccacgcc tgcggttgcc aggcattcgg caagcggcaa
     1021 atcttcggta ctgtataacg ctttttggaa aaaatcttcc aaatccaagc cggtaatttc
     1081 ctgacagcgg atttgccagt gtttttccgg aatacccgag tgtgtgtccc tccactcccg
     1141 atagagtttg tccattaacg tatcgagaga atgtctgccg ttgcttcggt tgcgtattat
     1201 cagatcaagg cacaatgcgg caagcgcgcc tttctggtag tagctgacga tggcgttggg
     1261 gctgttttca tccggtttgt aaaatttgtt ccacgcggta aaactcgatt ccgccaaggt
     1321 ctgcctcaaa cggccgcggg tttgttgtac gcgcgtaatg ccttgtgcca gcaggtttaa
     1381 ataagattcg ggcgagatgg tgcggctgcg tgccaaaaac aaatcgtcgt aataggatgt
     1441 aataccttcg aatgcccata gttgttcggt atagttttct ttgtcgaggt cataagggac
     1501 gaacgcggca ggtttgatgg atttgacgtt ccacgcgtga aaatattcgt gggagaaaag
     1561 tccgagcaat gtggtgtagg tatcgtcggc atcggtcata ccgtacggcg gaaggctgtg
     1621 gcggtcggcg agcagggcgg tgctgtcggt gtgttccaaa ccgccgtaaa tatggtcgcc
     1681 gacgtggagc aggaacaaat atttttgaaa cggggcaggg gaggaaaaca ccgccagttc
     1741 tgtttcgcag atttttttga tatccgaaac cagcctgttg cggtcgaaat cgggatatat
     1801 gccgcttaag gcaattgtgt gcggaatgcc tgccgcctca aaatctaaaa attcaatcaa
     1861 gcccatctcg acaggtcggt caatcaattc ggcataagat gccgcctgaa agacaaacct
     1921 ccctgtttcc ggcagcgttg tggcaatacg ccattcggac ggaatacccg tcaattccaa
     1981 gcggtgcggc agcgtttccg ttccttcgac tttcaaaaac aggcacgatc cgtcaaaaaa
     2041 accgcgttct gtcgtcagga aagaacctcg aaccgacaaa tcgaatgcat atacggtgta
     2101 gcggatttgc cactcgccgc gtacggcggc ggcatgccag cggtttttgg caatttgttc
     2161 gagcggcatg gacgtgccgt tacaggatgc atggatagaa gtgatgtggc gggaaaaatc
     2221 ccgaatcaga tagctgcccg gaacccagtt tggcaggctt atttctatag gaagatcatc
     2281 atcttgtgtg aatgtcagca ggatgtgcca ctcgtggtcg agaggggacg gggttatttt
     2341 ataatgaatc ataactatca aatatatcgt taaacgggat ggattaaata cctttaaaat
     2401 gcagcttaac ctacatcaat aaagtgccac aaaaagggaa aattggaaac aaaagcaatg
     2461 gcggtcgggt tgtaatttat tataacaata tgatttatat gtaataaaaa acagattaag
     2521 caaagggctc gtcggcggcg cgaatgtaat tccgcttggc gtaataaggc agatatgtta
     2581 aatttagaaa aaattacgct tgcccggatt gcaccttaaa caggctgcgg aagcggcagg
     2641 ataaaaatct ttattcccat aaattcatgg agactctcat ggacacacaa acttacaact
     2701 acaaagtggt gcgccagttc gccatcatga ctgtagtttg ggggattgtg ggtatgttgg
     2761 tcggcgttat cgtcgccgcc cagctttttg ctcctgccct cgacttgtct aatatcggac
     2821 cttggttcca cttcggccgc ctgcgtccgc tgcacaccaa tgcggttatt ttcgcatttg
     2881 gcggttgcgg cctgatcggc acatcatact acgttgtcca acgtacttgt aatacccgtc
     2941 ttttcggcgg ttggctgccg gcatttacct tttggggctg gcaggcggta atcgttgccg
     3001 ccgtcgtcag cttccctatg ggttggaccc aaggtaagga atatgccgaa ctggaatggc
     3061 cgatcgatat tctgattact ttggtttggg tggcttacgc catcgtattc ttcggtacga
     3121 ttgccaaacg taagattaaa catatttacg ttgccaactg gttctacggc ggctttattt
     3181 tggcggtcgc acttttacac atcgtcaaca atatcagcat cccggccggt ttgatgaagt
     3241 cataccccgt ctattccggt gcgattgatg ctatggttca atggtggtac gggcataatg
     3301 ccgtgggctt cttcctgact gccggcttct tgggtatgat gtactatttc gtacccaaac
     3361 aagcagcccg ccccgtttac tcctaccgcc tgtccgtcgt tcacttctgg gcgttgattt
     3421 ttacctatat gtgggcgggt ccgcaccatc ttcactacac tgcgctgcct gactggacgc
     3481 aatctttggg tatggttctg tctttgattc tgttcgcacc ctcttggggc ggtatgatta
     3541 acggcatcat gaccttgtcc ggcgcgtggg acaaactgcg tacagacccg attcttaaat
     3601 tcctgattgt atccttgtcc ttctacggta tgtctacctt tgaaggcccg atgatgtcga
     3661 ttaaaacggt caatgcattg agccactata cggactggac cgtcgcgcac gttcatgcgg
     3721 gtgcgttggg ctgggtaggc tttgtaacca tcggttccgt ctattacatg attccccgtc
     3781 tgttcggcaa agaacagatg cacagcacca agctggtaga agcacatttt tggattgcga
     3841 ccatcggcgt ggttctgtat atcgctgcca tgtggattgc cggtgtgatg cagggtttga
     3901 tgtggagttc tttgaacgat gacggtacgc tgacctactc gtttgtcgaa tccgtaaaac
     3961 gcaccatgcc ttactacgtg atccgttttg caggtggttt attgtatctg agtggtatgt
     4021 gcattatggc gtacaacgtg taccgcacag ccatcggtgg taaagcagtc gatgccgaaa
     4081 tccctgcggt ttcccaaaca cagcaccact aaaatactaa gaaagatagg ctaccaaaat
     4141 gaaattacaa caattggctg aagaaaaaat cggcgttctg attgtgttca cgctgcttgt
     4201 agtcagtgtc ggtctgttga ttgaagttgt gcccttggcc tttaccaagg cggcaacaca
     4261 gccggcgccg ggcgtgaagc cttacaatgc cctgcaggtt gccggacgcg atatttacat
     4321 ccgtgagggc tgttacaact gccactcgca aatgattcgt ccgttccgtg cggaaaccga
     4381 gcgttacggt cattactctg ttgccggaga gtcggtttac gaccatccgt tccaatgggg
     4441 ttccaaacgt accggtcctg atttggcacg tgtgggcggt cgctattccg acgaatggca
     4501 ccgtatccac ctgctgaatc cccgtgatgt cgtgcctgag tccaatatgc cggcattccc
     4561 gtggcttgca cgcaataaag tcgatgtcga tgcaaccgtt gccaacatga aggctttgcg
     4621 taaagtaggt actccttaca gtgatgagga aattgcgaaa gcacctgagg ctttggcaaa
     4681 caaatccgag ctggatgctg tagtcgccta tctgcaagga ttgggtctgg ctttgaaaaa
     4741 cgtaaggtaa catcatggat attaacggta ttcgtgctct cttcacggta tggatcttta
     4801 tctgtttcct gttggtactc tatatcgtct tcaacaggcg gaataagaaa aactacgata
     4861 atgccgcaaa cagcattttt gatgaaaacc aagatgcgca agataagaaa agcgaaaacc
     4921 gttaatattg tgataacgga gcaaaacaat gaacacaaca tcccaattta ccagtaattt
     4981 ctggaatata tatattgcag ttattgtctt actgagcttt atcgctttgg cttggctgct
     5041 gctgtctcaa aatgttgtca aacgtccgaa gaagggcgaa gaagtacaaa ctacgggtca
     5101 tgagtgggac ggcattgccg aatacgacaa tccgctgccc cgctggtggt tttggctgtg
     5161 tgttttgacg tggctgttcg gtatcggtta tttggttatg tatccgggtg tcggcgacta
     5221 caaaggtctg ctgaaatgga ccagccataa ccaatatgaa aaagaggtca aaaaagccga
     5281 tgagcaatac ggcaaactgt atgccaagtt tgcggatatg ccgattgaaa aagtggcaaa
     5341 agaccctcaa gccaagcaaa tcgcccaaaa cctgtttaac acttattgta tccagtgcca
     5401 cggctctgat gctaaaggct ctaaaggttt tccgaatctg accgatagcg attggttgtg
     5461 gggcggtgat cccgataaaa tccacgagac catcgaaaaa ggccgtgttg cgactatgcc
     5521 tgcctggggt cctgctttgg gcgaagaagg cgtgaaagac gttgcccatt atgtgatgtc
     5581 cctttcaaaa cccgaaggtc agtatgatga ggaacgtgcc gcgcgcggac aagccttgtt
     5641 cagcggtccg cctgccaact gtttcacttg ccacggcgat aagggacaag gtatccaagg
     5701 tttgggtccg aacctgactg atgacgtgtg gttgtggggc ggtacgcaaa aatccattat
     5761 cgaaaccatt accaacggtc gcagcagcca aatgcccgct tggggacatt tcttggataa
     5821 agacaaactg catattatga ctgcttatgt atggggtctt tccgataaag acggtaaagc
     5881 tccggtgaaa aaagccgagc ctgcaccgac tcccgcaccg gcggcagaac ccgcagcctc
     5941 tgctcctgca gaagcagcac aagccgtgtc cgaagccaaa cctgccgcag cagaacctaa
     6001 agccgaggaa aaagctgcac ctgctgccaa agcggacggc aaataggttt atgaaaccgt
     6061 ttgtgccgcc tgccatggca atgcgattcc gggtattccc catgtaggca tcaaagccga
     6121 ttgggccgac cgcatcaaaa aaggcaagga cacgttgcac aaacacgcga tcgaaggttt
     6181 caatacgatg cccgcaaaag gcggtcgcgg cgatttgagc gatgatgagg ttaaagctgc
     6241 ggttgactat atggtcaacc agtcaggcgg caaattctaa cttgactgag tttccgatat
     6301 tatcggttat gaaaatccag attcagtatt gaatctggat tttttgtttt aacgggcatc
     6361 cggttttaaa cgcagactgc ctatttgttg taatgaattt tgactttagt cataaagaat
     6421 tagtctttat tgtttataat cgcgcatcat cacgatagcc ctattttcaa aaggacgatt
     6481 aatggataca caaatcaaaa ctgaagccga caatcagagc aaccggcgtt atctgaccgt
     6541 ttggcggtgg catttttatg ccggtctgct ggttatgcct tttcttaccc tgcttgccgt
     6601 tacgggtttg ggtatgctgc tgtttgccaa tattaccggt aaggagggcg agcggattca
     6661 tgttgtgccg caggcaacgg tacaacctct gtctgttcag gcggaagcgg cacgcagtgc
     6721 cgttaatccg gaaacttcgt ccgtcgttca gtatattgcg ccgcgtgccg atgatatggt
     6781 tgccgtgttc cgtgtcaaca atgagggcaa agcaacgatg gtcgcggtcg atccttatac
     6841 ggcaaaagtg gtcagtacca tgccgcgcaa tcagggttgg tattacacga tggatgaaat
     6901 ccacagcgat atgatgctcg gtgcggcagg cgattatctt ttggaaacgg cagcttcact
     6961 gaccattatt atggttgtca gcggcttgta cctttggtgg gtgaaacggc gcggcatcaa
     7021 ggcgatgctg ctgccgtcaa aaggcagggc gcgttcttgg tggcggaatc tgcacggcac
     7081 gtttggaact tgggtgtcgt tgattttgct gttgttctgc ctgtcgggta ttgcttgggc
     7141 gggtatttgg ggcggcaagt tcgtacaggc ttggagtcag ttccctgccg gtaaatgggg
     7201 tgtcgaaccg aaccccgttt cagtcgtgcc gacccacggc gaggtattga atgacggcaa
     7261 ggttaaggaa gtgccgtggg ttttggagct tacgcctatg cctgtttcag ggacgactgt
     7321 gggcaaagac ggcattaacc ctgacgagcc gatgacattg gaaaccgtcg accgctttgc
     7381 gcgggaaatc ggtttcaaag ggcgttatca gttgaatttg cccaaaggcg aggacggcgt
     7441 atggactttg tcgcaggatt ctatgagtta cgacatgatc agcccgtttg ccgaccgcac
     7501 ggtacatatc gaccagtaca gcggcaaaat ccttgccgac atccgttttg acgattacaa
     7561 cccgttcggc aaatttatgg cggcaagcat tgcgctgcat atggggactc tgggctggtg
     7621 gagcgtgttg gcgaacgtct tgttctgcct tgccgtcatt tttatcggta tcagcggctg
     7681 cgtgatgtgg tggaaacgcc gtccgaccgg agcggtgggc atcgttccgc cggcgcagaa
     7741 agtcaagctg ccggtttggt ggatgatggc attgccgcta ttggcaatcg cactgctctt
     7801 cccgaccgca ctgcttgcca ttgccgtgat ttggctgttg gatacgctgc tgttgtcgcg
     7861 gattcctgtt ttgaggagat ggtttaaatg accaaatgcc gtctgaaagg ttcagacggc
     7921 attttgtttg aaggcggacg ggggaaaggc tatataatcc cgaatacttg accacagctt
     7981 ctgtttgaaa tcatgtttta tctgtatcaa tccaaccgtc ttgaaacgct ggcggcattg
     8041 tttgcccgca ttcaaaaagt caaaccgctg aaatcggctt tacagcccga acagattatt
     8101 gtgcagagtc aggggatgcg ccgctacctc aatacctgcc tcgcccgcga tttgggcgtg
     8161 gcggcgaatt tgtcgttcag cctgcccgcc ggcctgacgt ggaagctgat gaaaaaactg
     8221 attcccggta ttccggaact cagcccgttt gcgcccgaag tcatgcgctg gcggctgctg
     8281 gatttgttcc gcagcgaggc atttcggaat acggcagaat ttgaagatgt gaggaatgtg
     8341 ctgcaagact atctgggcag cggcgaatcg gcagattacc agcttgcggg acagcttgcg
     8401 gacatattcg accaatacct cgtgtaccgt cctcagtgga tagacgcttg gcagcagggc
     8461 aggcggctcg gtttggatga cgacgaaatc tggcagtcca aactgtggcg ttacctcgac
     8521 gacggcaggc agagcgcgcc gcaccgtgtc gcgttgtggg aaaagctgtt ggaatctttg
     8581 agcagtgata agctgcccga gcgttatttc gtgttcggca tttccacgat ggcgccgatg
     8641 tatttgcaac ttttgcacaa gctgtccgaa cattgcgacg tgttcgtgtt cgcactcaat
     8701 ccgagcggga tgtactgggg caacgtcatc gaagcggcgc aaatcctcaa aggtggcggc
     8761 gatcccgatt taactcaggc agggcatccg ctgctcgctt cattgggcaa gcaggggcgg
     8821 gactttttcg actttttgaa cgaaatggaa atagaaggcg aaacgccggt atttgaggaa
     8881 ggcgggcgcg atacgctttt acacgccctg caaaccgata ttcaaaacct gaaaatgccg
     8941 tctgaaatgg cgggaagcgt caacacgggc gacggctcga tacgcatcgt ctccgcacac
     9001 agccctttgc gcgaattgca gatactcaaa gacaagctgt tgaaaattct gcatgaacat
     9061 cccgattggc agccgcacga tatcgccgta ttaaccccga atatcgaatc ctatacgcct
     9121 tttatcgaag ccgtgttcgg acaggcgcag cccggtgcgc aggcattgcc gtattccgtg
     9181 tcagacgtga aaatcagccg ccgccaaccg ctgtttcatg ctttgtcatg cctgttcgac
     9241 ttgttggaaa gccgatttga agtcgataaa gtgcttgtgc ttttagaaac cgcgcccgtg
     9301 ttgcgccgtt tcggactgac tgaggacgat ttaccgcttt tgcacgacat ggttgccgat
     9361 ttgaacgtcc actggggttt ggacggagaa atgcgcggcg gcacggatca gctgttcacc
     9421 tggaagcagg cggtagaacg catgatattg ggctggatgc tgcccaaagg cggcaatccg
     9481 atgtggcagg atgtcagcgc gtggtatgcc gacgtgaatc aaaccgccat gttcggacgt
     9541 tttgccgcct tcctcgaaac cctgacggat attgtacgga tatggcggca gcccgcaacg
     9601 gtcggcgaat gggtggcgcg ttgccgggat ttgcttgaaa cattgttcca agctgaagcc
     9661 gatgaccaaa agtcagtcca aaaccttgaa aacgaatggg tcaaatggca ggcggaaacc
     9721 gaattggcgc aattttccgg acagttgccg ccgcacaccg tcatccgcca tatccgacgc
     9781 tttctcgaca gcgaaagcga ggcaggcttt ttacgcggcg gcatcacctt ttgcagtatg
     9841 gtgccgatgc ggagcctgcc gttcaaagtc atctgcctgt tgggtttgaa cgacggagat
     9901 tttccccgta ataccaaagc cgccgtattc gacctgattg ccaaacatcc cgccaaagga
     9961 gaccgcgccc gccgcgatga cgaccgctac ctgttcctcg aagccctcat cagcgcgcgt
    10021 gaaatcctct acctgtccta catcgggcgc gacatccgca aagacgaaga gcttgccccg
    10081 tcttccctgt tgggcgaact catcgatacc gttgccgcta tggcgggcat cggtagccgc
    10141 caacttgcac aaaacctgat agaacagcat ccgctgcaag ccttctcgcg ccgatatttt
    10201 caagaaggcg gacgttcaga cggcatattc ggcacgcgta ccgactacgc cgccgcgctc
    10261 ggacaaacgc ccgaaccgcc gcaacccttt ttcgatcaac ccgtagaaaa cgccgaacct
    10321 gttgccgaaa tcggacagga cgaatttatc cgcttctggc gcaaccctgt caaagtatgg
    10381 cttcagcagc agcttgcgtg gagcgaaccc catatcggcg aagcctggga gcctgccgaa
    10441 cccttcgagc cgcaacacgc cgatcaaatc gccgaaatct acatcgaagc acggcgcgaa
    10501 ggacgggatt ttgcccaaac cgccgcccgc atcggggcgg aaagcctcct gccgtcggga
    10561 gagttgggca gactttggca gcaggacttc caaactgctg ccaaacaaat cgacacggcg
    10621 gttttaaaca gccccaaact gccaccgctt tcatatgcca taccgtcgga cgggcaaatc
    10681 ctgaaaggca gcttgggcaa tctgtaccgc tgcggacaag tgttttacgc ctacggcaaa
    10741 cccaacgcgc cgcaacgtat cgcttttctg ctggaacacc tgatattttg cgccgttatg
    10801 ccgtctgaag ccgaaacgcg gcaaaccttt atcgtccaat ccggagaaac cgaaatattg
    10861 gcggaaatcg cgcaagacag ggcattacag ctattgtcgg aatggatggc gtttttcaat
    10921 atcgggcaaa accgcccgct gccgtttttt gccaaaacct cgcttgccac cgccgaagcg
    10981 tttgcccaaa aacaagattg ggaagccgcc ctgaaaaaag cccaaaccgc ctaccacggc
    11041 aacaaagtca gcaaagggca gaaagactat accgaagtcg ccctcgtgtt cggcaacgca
    11101 agccaaaacc cgctcgaaca gcccctgttt gaaaacctcg tccgtctgct tgccgacacg
    11161 tttgccgcag cggaaaaaaa ggaagaggcc ggagcagcct gacgggatgg aaaggcaaat
    11221 gccgtctgaa aacagcaaaa aggttttcag acggcatttt gtttatcgtg tttaaggtgc
    11281 gggatacaaa gtgggtgcgc cgggaccgac gggcagaccc aaaacaaata cccaaatgca
    11341 gaataaggca atccatgcaa ttaagaagaa agcggaatac ggcaacatca tagaaatcag
    11401 cgtacccacg cccgcatctt ttttgtattt gatcaccgtc gccataatca gcccgaaata
    11461 actcatcatc ggcgtaataa tattggtaac ggaatcaccg atgcggtaag cggcttgaat
    11521 gacttcgggc gcgtagccgg ccaacatcag catagggacg aaaatcggcg cagttaccgc
    11581 ccattgcgcg gaggcggagc ctatcatcag attgataaaa gcacaaatta aaataaaacc
    11641 gataaacaac acgctgccgc ccaagccgac ttcttttaag aacgtcgccc ctttaacggc
    11701 aatatattgc ccaatattcg tccaattaaa aaatgcgaca aactgtgcgg caaaaaagat
    11761 gatgaccaaa taaagcccca gagtactcat cgattcggcc atcgcattaa cgacttcctg
    11821 ttcgccgcgc aaacttcggg ttacccggcc ataaacaatg cccggcagcg caaacaacaa
    11881 gaaaataaaa acaacaatcg attttaaaaa cggcgaaccg gaaaccaatc ctgtttcagg
    11941 atgacgcaaa ataccgtcgg cagggacgat gctccaagcc aatagggcgg ataaggcaac
    12001 aaacaccacg ccagcccaaa ttaatccttt atattccaaa ggcgtgattt cattggaatg
    12061 ccgaatgtct ttttcttctt gtgacaaatc tgattgataa gggcccaatt gcggttcgac
    12121 gattttttca gtaacaaaat aaccaatcaa agcaatcaca aacgtactgg ctaccataaa
    12181 aaaccagttg gcttcagggc ctacgacgta gtcgggatgg atgatttgcg ccgcctgttg
    12241 ggtgatgcct gccaagagcg gatcgattgt gcctaagaac agattggccg aataaccgcc
    12301 cgaaacgccg gcgaaagccg cagccagacc ggcaagcgga tggcggccga gggaatgaaa
    12361 gatgatggcg gacaaaggga ttaggacgac atagcccaat tcagaagcgg tattagataa
    12421 aatccctgta aaaacaacca taaaagtagt gagtttgcgt ggagattttg tgagcaataa
    12481 gcgcattaat gcggaaatca agcccgattt ttccgcaatc cccacgccca ataaagaaac
    12541 caacaccgtt cccaacggcg cgaaaccggt gaaattttta acggtatgcg tcaggatttt
    12601 gatcaaaccg tcggcattga gcaggctgac aacgtaaatc aaaccgtcat cggcacgtcc
    12661 tttcgcacca acagggcgcg gatcggggac ggatagtccg aaatacgcac cggcggcaga
    12721 ggcaatcagc aataacacaa tgaaaataat aaaaagcgta accgggtgcg gcaacatatt
    12781 gcccagccat tcgactgtgc gtaaaaatcg tccgccccgc cgcgtatcgg tttgattcat
    12841 ctgtttctcc ttaaaaaaat atatcgtgtg cattaaattt ttgaatgtaa catgaagtgt
    12901 tttgtaagca aactaaaata atcgcatatg tatataaccg caatagatta aaacagaaaa
    12961 tgccctcatc gttattatcg taaaggcggg aatccgaaag ctcgacgtta aagccttatc
    13021 aggaatgact ggaactcaaa aaacggtttc ccgctttcgc ggaaatggcg ggattttcgg
    13081 tttttatttg caaaaatgcc ggagaagaat gcagtttaca ttttgaatgg tattgttatt
    13141 aattgtaaat aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaatcatgga
    13201 caaatataat ccatggcatg aagatgattt tgagtcgtat gaagacattg ccaaagatgt
    13261 atcgctgatg acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga
    13321 aaacggacat tttgaccaag agaatgtcca tgcaatgata gaagaaatta aaaatgcaat
    13381 ttagtgaat
//
LOCUS       DAWHRS010000047        13081 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWHRS010000047 DAWHRS010000000
VERSION     DAWHRS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13081
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.047"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(57..1100)
                     /gene="galE"
                     /locus_tag="WIB82_000935"
                     /pseudo
     CDS             complement(57..1100)
                     /gene="galE"
                     /locus_tag="WIB82_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1093..2095)
                     /gene="dcm"
                     /locus_tag="WIB82_000936"
                     /pseudo
     CDS             complement(1093..2095)
                     /gene="dcm"
                     /locus_tag="WIB82_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(2126..4399)
                     /locus_tag="WIB82_000937"
     CDS             complement(2126..4399)
                     /locus_tag="WIB82_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ3842254.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVEGKETEGEKFSDYFDHREPVRTIPSHRA
                     LAVLRGRNEGILNIAIKYQPDDTPITQQSEYEQIIACRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQNQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            complement(4463..5113)
                     /locus_tag="WIB82_000938"
     CDS             complement(4463..5113)
                     /locus_tag="WIB82_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3842255.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(5110..5907)
                     /locus_tag="WIB82_000939"
     CDS             complement(5110..5907)
                     /locus_tag="WIB82_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ3842256.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5907..7070)
                     /gene="ctrB"
                     /locus_tag="WIB82_000940"
     CDS             complement(5907..7070)
                     /gene="ctrB"
                     /locus_tag="WIB82_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ3842257.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7085..8260)
                     /gene="ctrA"
                     /locus_tag="WIB82_000941"
     CDS             complement(7085..8260)
                     /gene="ctrA"
                     /locus_tag="WIB82_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ3842258.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8395..9528
                     /gene="neuC"
                     /locus_tag="WIB82_000942"
     CDS             8395..9528
                     /gene="neuC"
                     /locus_tag="WIB82_000942"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ3842259.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKCQSEKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            9532..10218
                     /gene="neuA"
                     /locus_tag="WIB82_000943"
     CDS             9532..10218
                     /gene="neuA"
                     /locus_tag="WIB82_000943"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ3842260.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10219..11268
                     /gene="siaC"
                     /locus_tag="WIB82_000944"
     CDS             10219..11268
                     /gene="siaC"
                     /locus_tag="WIB82_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ3842261.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            11352..12839
                     /locus_tag="WIB82_000945"
     CDS             11352..12839
                     /locus_tag="WIB82_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HEZ3842262.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
BASE COUNT         3789 a         3083 c         2662 g         3547 t
ORIGIN      
        1 ccgataaagc ccgcaccgcc ggtaacgagg atggtttttt tatttgcggt ttgcatttaa
       61 tccccgtatc cgtcggggtt gcggctgacc caacgccacg aatcttccat catttgtgca
      121 aggctgcgtt tggtttccca gcctgtctct tgtttggtat gggacgggtc ggcaaaggaa
      181 caagcgaggt cgccggcgcg tcggggttgg atttgatagg gaatatttaa gccggatgcg
      241 gcttcaaagg cacggatgat ttccaaaacg gaataggcgc gccccgaacc caagttgaac
      301 aaatgtacgc cggcaacgcc gccttttgcc tgcatagccg cgacatggcc ttctgccaaa
      361 tccatcacat gaatatagtc acgcatcccc gtgccgtcgg gggtagggta gtcatcgcca
      421 aataccgcca attgcggcag tttgcctgcc gccacttggc agatataagg caacaaatta
      481 ttcgggatgc cgtttggctg ctcgccaatc aaaccacttt cgtgcgcgcc aatcggattg
      541 aaataacgca acaaaatcac gctccagcgc ggatcggctt tttgaatatc agtgagaatg
      601 cgctcaacca ttgatttcga tgcgccataa ggattagcgg tatcgcccgg gcgcatatct
      661 tccgtatagg gcattttacg tatcatggcg cgacgcatta aagtaaacgc caaagcgcgt
      721 ttatactggt tatccagttt ccctatattt ttacgttaca aatcaagttg ttggtattca
      781 tcgtgaaaat agaatttttc ggcataacgc tgactcataa aaccggcatg cgggctgcct
      841 gaaaaaatca ttgcgacatc atcgtcattt aatgtttcat ggttgttttc tattaatgct
      901 aaagcaattt ggtaattaat tgccaaaata tcgttagtaa tcacacgata gccgcgtttt
      961 ttggcttcgt aggcaaagga acagcctcca ctaaatacat ctgcaactgt atctacatca
     1021 gacggaagct ggtcacaaat ccaggaagct attttctctt tattaccgat atagttaatt
     1081 ttcggatatt gcttattcat tttcactcat ttttaaaata ctttcagcaa tagcttttgc
     1141 caataaaggc ggtacggcgt taccaacctg ctgctgctgg gcaattttgc tgccgcaaaa
     1201 aataaaatta tcagggaaag attgtaaggc agctaattca cgaacggtta acgcccgatt
     1261 ctgttcatag tgaaaaactt tgcgcatatc tcctgtaata caaacggctg gttttgttgc
     1321 tgttgtaacg gatgtattta cggatatcac ctgttttcgg acgtaatggt tcaggaatat
     1381 cgttacggtt acctccattt ttaacaaatg ccattttttc taacatttgt gccgaatgat
     1441 tcatagcttc atgatttgca acgtgtggat tgctttcgcc agcagccagt tttggaaaat
     1501 gtcctattgc tgatccaaca gtctgatggg aaatctgcaa aggttcggga aaggaaattt
     1561 tgcctttatc cctcctcccg ataaatatca ctcggctacg tatctgagga acaccgaaat
     1621 cggctgcact cagtatctta cattccaccg aataaccgat attctgaaat gcttgaataa
     1681 tctcaatacg tgttttacct gaattgtgtg tatagagtcg cgctacattt tccataacaa
     1741 aaaaatatgg ttggacaatt ttaactattc ggacaaactc tttaaataaa tggttgcgtg
     1801 ggtcatctgt aaatgtccgt ccaatctttc ctgccatact aaaaccttga caaggtggtc
     1861 ctccaataat caaatcaact gcttgtccgt taagacaatt gattaaatct tgttcggtta
     1921 gtgtggttaa atctttttgc agtaattgat gatgggggaa gttggtacgg taagtctgac
     1981 aataatcaga ctccatttca acagaaagca attgttggaa tccggcttgt tcaaaaccca
     2041 aggataggcc tcctgctcct gagaaaaggt caatataggt aagtggtgtg tgcataagtc
     2101 aaaatccata aactcttcga ttattttacc gcttcagctt cgcaaacgca tccgccatcg
     2161 ccgaattggt cggggcgcgg tcgttgcgtt ggggtttgcg gccggctgtc cgttcgcggc
     2221 tgcggttttc agacggcatt ttatgttttg cgccgcccgg ttcgtcatcc aagcgcatgg
     2281 tcagcgcgat gcgtttgcgt gcagcatcga cttccagcac tttcactttc accacgtcgc
     2341 cagctttcac cacttcgcgc gggtcttgga cgaacttgtt ggacagggcg gagatgtgca
     2401 ccaagccgtc ctgatggacg ccgatgtcca cgaacgcgcc gaagttggcg acgttggaaa
     2461 ccacgccttc gagtatcata ccgacttgca agtcgctgat ttcgtggata ccttcggcaa
     2521 acgatgccgt ctgaaactcg ccgcgcggat cacggccggg tttttccagt tcggacagga
     2581 tgtccaaaat ggtcggcagg ccgaagcgtt cgtcggtgaa gtcggacgct ttgatttgct
     2641 tcacgcgctc gcggttgccg atgagttcgg cggcgctaat gccttgttgc gccagcattt
     2701 tggcgacgac gggataggct tcggggtgga cggcgctcgc gtccaacggc tctttaccgc
     2761 cgttaatccg caaaaagcct gccgcctgct cgaaggtttt ttcgcccaaa cgcggtactt
     2821 tcagcaattt tttgcggctg tcgaacgcgc cgttttcatc gcggtaggca acgatgtttt
     2881 gggcaagggt ttgattcaag ccggaaatcc gcgccaagag cggggcggag gcggtattca
     2941 cgtccacgcc gacggcgttc acgcagtctt cgaccactgc gtccagcgat ttggcgagct
     3001 ggttttggtt cacatcgtgc tgatactggc ccacgccgat ggatttaggg tcgattttga
     3061 ccaactcggc aagcgggtct tgcagcctgc gggcgatgga caccgcgccg cgcagggaaa
     3121 cgtccaagtc ggggaactcg cgcgccgcca gttcggacgc ggaataaatc gacgcgccgg
     3181 cttcggacac gacgatttta tgcagcccca ttttcgacat ttcacgcacc agttcgcccg
     3241 cgattttgtc ggtttcacgg ctggcggtgc cgttaccgat ggcgatgagc ttcacgccgt
     3301 gctgtttaat caggcgcgac agcgttgcca acatattgtt ttcttgatgc aaatagacga
     3361 tgacggtatc cagcagcttg ccggtgtcgt ccaccacggc gcatttcaca ccgttgcggt
     3421 agccggggtc gagacccagc gtggtcagcc gtccggcggg cgcagcaagc agcaagtctt
     3481 tgagattgcg ggcgaacacg gtaatcgcgt cggtgtcggc ggcttctttc aaacgattga
     3541 gagcttcaag ttccagcgac aaaaagattt tcgcgcgcca agtcaggcgc acggtgtcgc
     3601 gcagccattt gtggccgtct gaaaccttga aacggcaggc gatgatttgc tcgtattcac
     3661 tttgctgcgt aatcggcgtg tcgtcgggct gatatttgat cgcgatgttc aaaatgcctt
     3721 cgttgcggcc gcgcaatacc gccagcgcac gatgactggg tatagtgcgg acgggttcgc
     3781 gatggtcgaa ataatcgctg aatttttcgc cttcggtttc tttgccttca acgacttgcg
     3841 cgtgaatttc ggcttcgttc cacagcttgt cgcgcagcgt gccaatgagt tccgcgtctt
     3901 cggcaaactg ctccatcaga atcgcgcgcg cgccgtccag cgcggccttg gcatcgggga
     3961 cgttttcgtt caggtagcct tgcgcggcag cttccacgtc ctgcggctgc tcggcaagca
     4021 acacgtccgc cagcggctgc aaaccgtgtt cgcgcgcaat ttgcgctttg gtgcggcgtt
     4081 tgggcttgta gggcagatac aggtcttcca gcgcggtttt gttgtcggcg gcttcgattt
     4141 gcgccctgag gtcgtctgaa agcttgcctt gctcttcaat gctttttaaa acaacggctt
     4201 tgcgctcttc caactcgcgc aggtattgca gccgctcggc aagctggcgc agctgcgtat
     4261 cgtccagccc gcccgtggct tccttgcgat agcgggcgat aaacggcaca gtcgcgccgt
     4321 cgtccaaaag ctcgacggcg gcagtgattt gcgcggcggt ggcggagagt tcttgggaaa
     4381 gaatttgagt aatgttcatc aatagaattc caacggacag gccgtctgaa atttcagacg
     4441 gcctgattta aaaacaatcg ctttaaggca gcgaattata atattcgtag gctttgtcca
     4501 tatcttcaaa ctggtacata tgcccttttt ccagcaccat cgcattatcg caatattgct
     4561 tcatggcgct gtggctgtgc gacaccaaga tgatggaacg gtctttgcgc ttttcaaaca
     4621 actcgtactt acatttatcg gcaaaacgcg agtcaccaac tgcaatcact tcgtcaatca
     4681 aatagcaatc aaactctacc gccaacgaca aagcaaatgc caaacgcgcc ttcatacctg
     4741 aagaataacg cttgaccggc tcgtaaagat attgccccaa ctcggaaaac tcttcggtaa
     4801 agttcttcac ataatcgata tcgacattgt aaatccggca gatgaaacgc aaattgtcca
     4861 taccggtcag actaccttga aacgcaccgg agaatgccaa aggccaagaa atactcattg
     4921 tccgcttgat ttcacccgtg gtcggcggct caacgccact gatcaaacgg atgagcgtcg
     4981 atttacctgc accgttgcgg ccgagaatac cgattttctc gcccttctcc attttgaagc
     5041 taatatcgtg caagactgtc cgccaacctt ggcgggtcag atagcgtttg gaaacgtgtt
     5101 caactgaaat cattgcggct caaccccttt gctgaatctc gccaccaccg ccaaaccaag
     5161 cagcagcaaa accaagttac acaacaaaat ataccaagga ttttcgtaag tcgtgacgct
     5221 gtcgccaaaa tatccggcac ggaacatttc tgtgccatgc accatcggga tcatcaatgc
     5281 atactcttgc actttcggag gcaaattatg tacaaaaaag aatgcacccg ataacggcat
     5341 catcacaaaa gtcaatgtgc cccaaatctt gccaaacggc tcgaaattaa aggcaatcga
     5401 acaaatcacc aaacccaaac caatcgcaaa aaaagccatc aaaagccaag ccatcagcat
     5461 ataaaacata tctgccggca tttcaatcca gccaatcgca atcaataccg ccataatcac
     5521 aatctgcgca atggttgcac cagcaatttc caaaatcatg cgcgccaaga tggtatccaa
     5581 aactcttaca ttgcggtgat aaagcaagct ggcatttgaa gaaatcgacc caactgcccg
     5641 ttttgaggca ttacgccaca tcatcaacat cggatagcca gtaatcgcaa atgcgacaat
     5701 attcaaagtt gaatatcggt ctgcccttaa aaatttccac atcaagacga taacgaatgt
     5761 catcagcaac ggctcaacaa acagccataa aaagccaata ttattgcgac cgtaacgggt
     5821 gataatttcc cgcatcaaca gcgcaccgat tacgcgcctt tgaatggcta aagattccca
     5881 aaatgatgtt ttatgcaagg ctttcatcag tttttatgct cacgaatgct ggcagtcaac
     5941 aggctcaaaa taccataaac catcaagccg ataatcagag tggcaacaat gttgtataac
     6001 cgtttaggct catgtgccaa atccggcagg ctcggttgcg agatcacttc caaataaagc
     6061 tgctgacggt ctgcttcaac cttggcactt tccaaagaag tcatggccgc tgccaattgc
     6121 tgctcagcta attgattttc caaatacaca cgctgatatt cggcagcctg attagacaac
     6181 gaagaatgcc caccgcccga aatggcacgt aactgttggt caatttcttt acgcaagctc
     6241 tgctcacgcg cctgcaaacc cggaatctgc ggattctccg gagtgactgc tttcacctga
     6301 tccagctggg tttgaatcac aatcaattca tcttgcagct tggaaaccaa ccccatttgc
     6361 acttccgatt gcgctttcaa atcaaaaacg ccattggcaa tccggtaatc cgtcagattc
     6421 tgagaggctt cctttacccg ctctgccgcc gttttcacta cttcttccgc atagcgcacc
     6481 gtatcagcac gtgcacgatc gttcaactgg ttaatcaatg cttcaccttg ttttaacaaa
     6541 gcctcattga ttttcttaga ttccagcgca tcaaaggaag ttacattcaa cgtggaaata
     6601 cccgaaaccg tatcaaaatt gatcatcacc tgatttttat agtattgata aaaagcctct
     6661 tcctcgccac ggaacccaaa cccattaaag cggctgaacg catcaccttt ggtttcataa
     6721 aactcacgca ccggcaagat tttacgcagt tcatccaaag acgagcgcga acgcatatac
     6781 tccccaaccg tgtaaatatc atcttgcgca cgggcaaaac ctgtgccctg caaaatggca
     6841 cccaggccat tgagagaaga ttggctttta ggcgagcgca ccacaaagct cgattgcgac
     6901 gtaaaacgat cggaagcgaa gaagccgaaa tacaccaacg aaattaccgt agggataatc
     6961 accgttaccc aaaataaagg gcttagcttt ttaatccaac ttttcttttt cggcttttta
     7021 cgctcggctt tggtttcagt ggcaactgcc acaggaagtt gctcagacat cttaattact
     7081 cacattaatt agttaaatta ttaatactgt tcgcgccact ggtaaccggc gagaacacaa
     7141 acgacaagaa tttctgcact tcagccaacg gcgcattcga cacatacaat acatctttat
     7201 tcttcacagg aaagcgctgc atagaaaata gtgaatgcgc atcagccata ttcacacgat
     7261 ataccgttgg aatctctgcc tcactgccat aaccttgagc aatccattta tcctgacgtt
     7321 ctgccggcaa ttccaccaat ggcgtatagc ggaacacaaa cacaccacgc gcatcagaac
     7381 ggcgatcttg caaaccgccc atacggccaa tggcttcaga aagcgataag cctctggctg
     7441 aaaaaccgat ttcttgtgtt ctccccaccg cacccataga cgtaaaggta tagggattgg
     7501 taatcatggt aaccacatca ccgcgacgca gcaaaatatt ttgtcgcgga tttgcaacta
     7561 aatcttccaa ggcaacagtt cgtactacat tgccacgtgt cagctgcaca ttcgtatcct
     7621 gcacatttgc cgttgaacca cctaccgcag ccaccgcatc caacacacgc tcaccggctg
     7681 ccgtcagcgg catacgcaca ctattcccag cacgaatcac cgacacattc gccgcattat
     7741 tctgcaccaa acgcaccatc acttgtggct gattggccat ttttttcagg cggcctttaa
     7801 taatttcctg aacctgacca ggcgttttac cgaccaccga aatatcgcca acaaacggca
     7861 cagaaaccgt accacgtgcc gtgaccaact gctctggcaa cttagtttga tgcgcactac
     7921 ccgagcccat cgaagaaagg ccaccaccaa acaatactgc cggcggcgct tcccaaatca
     7981 taatatccaa tacatcacca atatttagcg taccagccga agcataacca tcgccaaact
     8041 gagtgaatga ctgatttatc tgagccttat ataataactg agcaaccgta tgattcacat
     8101 caatcagctc cacttcagga atttgaactt cagattgttg ccctaaagag acaatttttt
     8161 ttgcgctggg gcctgatgaa ggaatcgcag agcatcctac aattaaactt ccacacaata
     8221 ataatactgc gtgacgaata taaaatttca ctttaaacac aagccaaatc ctaatataat
     8281 tataaatggc ctaattatag cacttaatcg aaataaattt atgagtacgt agagtataat
     8341 tagtattctt ctttccaact tccttatact tatatactta tagattctaa aatcatgaaa
     8401 agaattcttt gcattacagg taccagagcc gacttcggca agctaaaacc tttattagcc
     8461 tatattgaaa atcacccaga ccttgaattg catttgattg taactggtat gcatatgatg
     8521 aaaacatatg gcagaacctg caaggaagta actcgagaaa actatcaaca tacatatctg
     8581 ttttcaaatc aaatccaagg tgaaccaatg ggtgccgttt taggcaatac cattacgttt
     8641 atctctcgtc tatctgatga aattgaacct gatatggtca tgattcacgg cgaccgttta
     8701 gaagcactag caggcgcaac tgtaggtgca ttaagcagcc gtttagtttg ccatatcgaa
     8761 ggtggtgaac tatctggtac agtagatgac tccattcgtc attctattag taaactttct
     8821 catatccact tggtagcaaa tgaacaagct gtcactcgcc tagtgcaaat gggagaaaaa
     8881 agaaagcata ttcacatcat cggctccccc gatttagatg ttatggcctc ttccaccctc
     8941 ccatccttag aagaagtcaa agaatattac ggtttaccat acgaaaatta tggtatttct
     9001 atgtttcacc ccgtgactac agaagcacat ttaatgccac aatatgcggc ccaatatttc
     9061 aaagcattag aattaagtgg ccaaaatatc attagcattt accctaacaa cgacacgggt
     9121 actgaaagta ttttacaaga gttattaaaa tgtcaatctg aaaaatttat cgcatttcca
     9181 tctatacgat ttgaatactt tttagtctta ttgaaacatg ctaaatttat ggtcggcaac
     9241 tcaagtgcag gtattagaga agctcctctc tacggtgtcc cttcaattga tgttggtaca
     9301 cgccaaagta accgccatat gggaaaatct attattcata cagattatga aactaaaaat
     9361 atctttgatg cgattcaaca agcatgcagt ttaggcaaat ttgaagcaga tgataccttt
     9421 aatggcggag atactcgcac cagcacagaa agatttgctg aagtaatcaa caatcctgaa
     9481 acgtggaatg tttctgctca aaaacgtttt atcgatttga atctttaaat tatggaaaaa
     9541 caaaatattg cggttatact tgcgcgccaa aactccaaag gattgccatt aaaaaatctc
     9601 cggaaaatga atggcatatc attacttggt catacaatta atgctgctat atcatcaaag
     9661 tgttttgacc gcataattgt ttcgactgat ggcgggttaa ttgcagaaga agctaaaaat
     9721 ttcggtgtcg aagtcgtcct acgccctgca gagctggcct ccgatacagc cagctctatt
     9781 tcaggtgtaa tacatgcttt agaaacaatt ggcagtaatt ccggcacagt aaccctatta
     9841 caaccaacca gtccattacg cacaggggct catattcgtg aagctttttc tctatttgat
     9901 gagaaaataa aaggatccgt tgtctctgca tgcccaatgg agcatcatcc actaaaaacc
     9961 ctgcttcaaa tcaataatgg cgaatatgcc cccatgcgcc atctaagcga tttggagcag
    10021 cctcgccaac aattacctca ggcatttagg cctaatggtg caatttacat taatgatact
    10081 gcttcactaa ttgcaaataa ttgttttttt atcgccccaa ccaaacttta tattatgtct
    10141 catcaagact ctatcgatat tgatactgag cttgatttac aacaggcaga aaacattctt
    10201 aatcacaagg aaagctaaat gcaaaacaac aacgaattta aaattggtaa tcgttcagta
    10261 ggttacaacc acgaaccatt gattatctgt gaaatcggca tcaatcatga aggctcttta
    10321 aaaacagctt ttgaaatggt tgatgctgcc tataatgcag gcgctgaagt tgttaaacat
    10381 caaacacaca tcgttgaaga cgaaatgtct gatgaggcca aacaagtcat tccaggcaat
    10441 gcagatgtct ctatttatga aattatggaa cgttgcgccc tgaatgaaga agatgagatt
    10501 aaattaaaag aatacgtaga gagtaagggt atgattttta tcagtactcc tttctctcgt
    10561 gcagctgctt tacgattaca acgtatggat attccagcat ataaaatcgg ctctggcgaa
    10621 tgtaataact acccattaat taaactggtg gcctcttttg gtaagcctat tattctctct
    10681 accggcatga attctattga aagcatcaaa aagtcggtag aaattattcg agaagcaggg
    10741 gtaccttatg ctttgcttca ctgtaccaac atctacccaa ccccttacga agatgttcga
    10801 ttgggtggta tgaacgattt atctgaagcc tttccagacg caatcattgg cctgtctgac
    10861 cataccttag ataactatgc ttgcttagga gcagtagctt taggcggttc gattttagag
    10921 cgtcacttta ctgaccgcat ggatcgccca ggtccggata ttgtatgctc tatgaatccg
    10981 gatactttta aagagctcaa gcaaggcgct catgctttaa aattggcacg cggcggcaaa
    11041 aaagacacga ttatcgcggg agaaaagcca actaaagatt tcgcctttgc atctgtcgta
    11101 gcagataaag acattaaaaa aggagaactg ttgtccggag ataacctatg ggttaaacgc
    11161 ccaggcaatg gagacttcag cgtcaacgaa tatgaaacat tatttggtaa ggtcgctgct
    11221 tgcaatattc gcaaaggtgc tcaaatcaaa aaaactgata ttgaataatg cttattaact
    11281 tagttacttt attaacagag gattggctat tacatatagc taattctcat taatttttaa
    11341 gagatacaat aatgctaaag aaaataaaaa aagctctttt tcagcctaaa aagttttttc
    11401 aagattcaat gtggttgaca acatctccat tttatcttac ccccccacgt aacaatttat
    11461 ttgtcatatc taatttaggt cagcttaacc aagtccaaag cctaattaaa atacaaaaat
    11521 taaccaataa tttactagta attttatata cttctaaaaa cttaaaaatg cctaagttag
    11581 ttcatcaatc agctaacaag aatctatttg aatctattta tctatttgag cttcctagaa
    11641 gccctaataa tataactcct aaaaaattac tttatattta tagaagttac aaaaaaatcc
    11701 ttaatattat acagcctgct catctctata tgctgtcttt tacaggccac tactcctatc
    11761 tgattagtat tgcaaaaaaa aagaatatta cgactcattt aattgatgaa gggactggaa
    11821 catatgctcc tttattagaa tcattttcat atcatccaac aaaattagaa cgttatttga
    11881 ttggaaataa tcttaatatt aaaggatata tagatcattt tgacatattg catgtcccct
    11941 ttcctgaata tgctaaaaaa atatttaatg caaaaaaata taaccggttt tttgcgcatg
    12001 ctggaggaat aagcattaat aataacattg caaacttaca gaaaaaatat caaatatcta
    12061 aaaatgacta tatttttgtt agtcaacgct accccatttc agatgatttg tattataaga
    12121 gtatagtaga aatcttaaac agcataagtt tacaaattaa aggaaagata tttattaaac
    12181 tacacccaaa agagatgggc aacaactatg taatgtcttt atttctaaat atggtagaaa
    12241 taaaccctcg gctggtagtt attaatgaac ctccttttct aattgagccc ctaatatact
    12301 taacaaatcc taaaggaatt ataggcctgg cctctagttc tttaatttat acaccattac
    12361 tctcaccctc aacccaatgt ctttctattg gagagttaat tattaactta attcaaaaat
    12421 attcaatggt ggaaaacact gaaatgatcc aagaacactt agagattatt aagaaattta
    12481 attttattaa tatactaaat gatttaaatg gggtaataag taaccctctc tttaaaacag
    12541 aagaaacatt tgaaacactt cttaaatctg cagaattcgc atataaatct aaaaactact
    12601 ttcaggctat tttttactgg caacttgcca gcaaaaacaa tattacctta ttagggcata
    12661 aagcattatg gtactacaat gcactttata atgtaaaaca aatttataag atggaatatt
    12721 cagatatttt ttatatcgat aatatctccg tagactttca tagtaaagat aaattgacat
    12781 gggaaaaaat taaacattat tactattccg ccgacaatag aattggtaga gatagataaa
    12841 aatactatga cttctgatct ccatacgtat gcttgttttc aaaaagcagc attaagatag
    12901 ataagtgaag cttgatgaga gccatttttg tggtcatcgc caaggtacac gcggagagta
    12961 atcgacaaca gagctgctgt attctggctt ttaaagccca gtagtaaagt ttataaggct
    13021 gagacctttg caaaatcccc caaaatcccc taaattccca ccaagacatt taggagattt
    13081 c
//
LOCUS       DAWHRS010000048        12969 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWHRS010000048 DAWHRS010000000
VERSION     DAWHRS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.048"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(53..361)
                     /locus_tag="WIB82_000946"
     CDS             complement(53..361)
                     /locus_tag="WIB82_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842263.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WIB82_000947"
     CDS             complement(345..647)
                     /locus_tag="WIB82_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ3842264.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WIB82_000948"
     CDS             complement(721..3084)
                     /locus_tag="WIB82_000948"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ3842265.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQIDAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGNVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="WIB82_000949"
     CDS             complement(3120..3638)
                     /locus_tag="WIB82_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842266.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="WIB82_000950"
     CDS             complement(3645..4709)
                     /locus_tag="WIB82_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ3842267.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WIB82_000951"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WIB82_000951"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ3842268.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WIB82_000952"
     CDS             complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WIB82_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ3842269.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WIB82_000953"
     CDS             complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WIB82_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ3842270.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WIB82_000954"
     CDS             complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WIB82_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ3842271.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WIB82_000955"
     CDS             complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WIB82_000955"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ3842272.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEQRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9446..9522)
                     /locus_tag="WIB82_000956"
     tRNA            complement(9446..9522)
                     /locus_tag="WIB82_000956"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9486..9488),aa:Val,seq:gac)
     gene            9701..10816
                     /gene="tgt"
                     /locus_tag="WIB82_000957"
     CDS             9701..10816
                     /gene="tgt"
                     /locus_tag="WIB82_000957"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ3842273.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            10826..11908
                     /locus_tag="WIB82_000958"
     CDS             10826..11908
                     /locus_tag="WIB82_000958"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ3842274.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGSWR"
     gene            12204..12605
                     /locus_tag="WIB82_000959"
     CDS             12204..12605
                     /locus_tag="WIB82_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ3842275.1"
                     /translation="MNTTRLPTALVLGCFCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKSKKN"
     gene            complement(12668..12958)
                     /locus_tag="WIB82_000960"
     CDS             complement(12668..12958)
                     /locus_tag="WIB82_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842276.1"
                     /translation="MAKYFTETGISVREHFDFFGEFVVSPAARSGDLALTYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVEGGRIDIGGFMLTAKEVDGGGNIGSMGLKVLR"
BASE COUNT         3274 a         3422 c         2893 g         3380 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg attatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgacgttgcc
     1141 gtctgaaacg atattggcgg cgcgtccggg atgcagggca ggatgtccgg ttttaacgaa
     1201 ctcgaccgct ttgtttttca acagattttc cacatctgcc ttaatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttcggg cattgccgcg ccgtaccaca agccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccgcgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggcccaa
     1861 acgcaatccg acctgcttcg cttcgggcaa ttcgccttgc gcttccacca tttcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgc tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatccg gcgtagtagc
     2401 gcgcgcgttc acgttttcaa tcacacggct gataaagcga ccgcaatcgg caggcgcgtc
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacaccacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tgcccggcaa cactgcgccc ggcaacgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttcg gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgaa taggagaatt gcatgttatg ttctcaatta actaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggcccg
     4681 aaattttcaa gccaaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagaca cattgtgaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctgcttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaag tcgttcacgt tgtaacgcag catagcgaag cggtcgagac
     4981 caataccaaa ggcgaaaccg gtatattttt cagggtcgat attgacgttt ttcaacacgt
     5041 taggatgtac cataccgcaa ccgccgactt ccagccattt gccgttttcg cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgctttcaaa tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatcg ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaatg tcgagggctt cggcggcaag tcgggcttgc agcttggctt
     5641 cgttgagggc atcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggttttg ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatataccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatctgt
     6061 actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata ttttaacgca
     6121 aatttattta aaaaaataga ctttaaagaa agaaaatcaa tcacatagaa tttctgcaaa
     6181 caaagaaaaa aggaagccgc agcttccttt tcaatttttt ggattaagca gccaaagcag
     6241 ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc gaacacggcc aaatcagcca
     6301 atactttgcg gtcgatttcg atagaggcgc gtttcagacc attcataaat ttgctgtaag
     6361 acaacccgtt ttcacgcgca cctgcattga tacggacaat ccacaattga cggaattggc
     6421 gtttgcgttg gcggcggtcg cggtatgcgt attggccggc tttcattacc gcctgcttgg
     6481 caacgcggta aacgttttta cgacggccgc ggtagccttt ggctaacgcg aagatttttt
     6541 ggtgacgggc acgggcggta acaccgcgtt ttacgcgtgg catattctaa actccttaag
     6601 cgtagggtaa cattttagca acagaagcca aatcgcgatc gtttaccata gaggtaccgc
     6661 gcagttggcg tttgtttttg gtggtctttt tagtcaagat gtggcgtttg aacgcatgag
     6721 cgcgtttcac accgccgtta cccagtactt taaagcgttt tttcgcgcta gacttggttt
     6781 tcattttagg catgggaaaa ctccattcgt tatcggataa ggcattaggg ggttttaaaa
     6841 catcagtttc aaacacttga acccacaata ccacggtttt ttgccgcaat tgaaaactta
     6901 ctccgaagcg gcaatcggag taagcggaga attatagctt tattttttct tcggcgcaat
     6961 catcatcacc atttggcgac cttccatttt gggaaaggac tcgatttgcg ccacttcagc
     7021 caaatcttct tttacacgtt ccaaaagttg cgcgccgagt tgctggtgag ccatttcacg
     7081 gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg gcaaggaagc ggttaatgtt
     7141 gcgcatcttg atttgataat cgccctcatc cgtacccgga cggaatttga tttccttgat
     7201 ttgcacctgc ttttggtttt tcttggcttc gtcgcgtttc ttggcctgct ggtatttgta
     7261 tttaccgtaa tccatcagtt tacacacagg cggtttagca gttggggaaa tctctaccaa
     7321 atcgacatcc tgcccttcgg ccatagccaa agcttcacga actgaaacga caccaagctg
     7381 ttcgcctgac tcactgatta aacgcacttc tttggcggta atttcgccgt tgattcgtgc
     7441 ttcgcgttct tgagcgatga tgaatactcc tataaaaatt aatgattgac gagggcatca
     7501 gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat ccaaagaacc caaatcttct
     7561 gctttgcggc gtaccgccac tttgttttcc tgcttttctt tatcgccgac aacgatttga
     7621 taaggaaaac ggtattggct gttgtcgcgg attttgtaac cgattttttc gttacgcaaa
     7681 tccaactcgg cgcggaatcc tgccgcctgc aatttggcag ccacttcacg gcagtaatcc
     7741 gcttgatttt cggtgatatt cataatcacc atttgaaccg gcgccagcca taacgggaaa
     7801 gagcctgcat ggttctcaat cagaatgccg ataaaccgct ccaaagaacc caaaatggcg
     7861 cggtgcaaca ttacagggcg agcgcggtca ttgttttcgg ttacatattc tgcatccaag
     7921 cgttccggta agacgaaatc tagttgcaat gtaccgcatt gccaagaacg acccaaggca
     7981 tctttgacgt gatattcgat tttaggcccg taaaacgcac cttcgcccgg caattcgccc
     8041 cattccacgc cgcaggcagt caatgcctcg cgcaaaccct gctctgcctt atcccacacg
     8101 tcatctgaac ctgcacgttg ttcagggcga agagaaagct tgacggatac atcatggaaa
     8161 ccgaactgtt tgtagatgcg aaccaacaat tcattgaatg cgcgcgcttc gctgacgatt
     8221 tggtcttcgg tacagaaaat atgcgcatca tcttgcacaa aaccgcgaac acgcatcaga
     8281 ccatgcaatg caccgcttgg ttcattgcgg tggcaagaac cgaactcggc caaacgcatc
     8341 ggcaaatcgc gatatgagcg caaaccgtta ttaaaaattt gcacatgacc cggacagttc
     8401 atcggtttca ccgcgtattc gcgtttttct gaactggtca cgaacatatt gtctttgtag
     8461 ttgtcccaat ggccggattt ttcccaaaag gttttatcca taatttgagg cgttttgacc
     8521 tctttataac cggcggcgtt cagctcttta cgcatatgct gttcaatcac ttgccacaaa
     8581 gcccagcctt taggatgcca aaacaccatg cccggcgctt cgtcttgcag atggaacaga
     8641 tccaattgct tgccaagttt gcggtggtcg cgcttttcgg cttcttcgat acgttgaata
     8701 taggctttca actcgtcttt tgtcgcccaa gccgtaccgt atatgcgttg cagcatttca
     8761 ttgttgctgt cgccgcgcca gtatgcgccc gccaacttgg tcagtttgaa gtttttcagg
     8821 aaacgggtat tcggaacgtg cggtccgcgg cacatatcga catattcctg atgatgatac
     8881 atccccatcg cttccacttc gggcatatcg tcaatcaggc gcagtttgta ttcttcgccg
     8941 cgctcttgaa aaattttaat cgcctccgca cgcggagtca tgattttgac cacatcatag
     9001 tcttgggcaa tcaattcttt catacgcgct tcaatggcgg caacatcttc cggtgtaaac
     9061 ggtttttccg tggcgatgtc gtaataaaag ccctcttcaa tgacggggcc gataaccatt
     9121 tttgcattag gatagagttg cttgacggca tgcccgacaa gatgggcgca ggaatggcgg
     9181 atgatttcga tgccttcctg atctttcgga gtaatgattt gaacagcaga attttcaaca
     9241 atcgggtcgc acgcatcgac caatttgccg tttaccctgc ctgccaccgc cgccctcgcc
     9301 aaaccgacac cgatagacgc agcaatttga gccacggtaa cgggggattc gtattggcgg
     9361 actgagccgt ccggcaaggt aatgttcaac atcaaaaagc tccaaaacat taaaaaataa
     9421 cggcataaat gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc
     9481 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgcatt
     9541 ttatcgggtc gttttaattt ttgcaagagg tatgtgcatt tctttatgca acattgtggc
     9601 aagatttggg gatgtcgtct gaaaaaccgt gggaattcat ttataatgcc gtcttttaaa
     9661 acatatttcg ccccctgtcg cccaactgac attcaatctt atgcttaaat ttaccttaca
     9721 caaaaaagac ggatacgccc gacgcggcac gctggagttg aaccacggca aaattgaaac
     9781 gccggtattt atgcctgtcg gtacttacgg ctcggttaag gcgatgaacc cgcaaaacct
     9841 gcacgacatc aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg
     9901 cttggaggtc gtcgaacagt ttggagggct tcacggcttt atcggctggg acaaaccgat
     9961 tctgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgacgga
    10021 agaaggctgt acgttcaaaa gcccgatcaa cggcgacaag ctgtttttat cgcctgaaat
    10081 ttccatgaaa atccaaacgg ttttgaactc ggacatcgcg atgcagctgg acgaatgcac
    10141 gccgggcgaa gcaacgcgcg aacaggcgaa aaaatcgctg caaatgagcc tgcgctgggc
    10201 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcgctgt tcggcatcgt
    10261 gcaaggcgcg atgtatgagg atttgcgcga agaatcgctg cgcggtttgg aacagtttga
    10321 ttttcccggc cttgccgtcg gcggtttgtc cgtcggcgaa cccaagcccg aaatgtaccg
    10381 tatgctgcgc gccgtcggtc cgattctgcc ggaacacaag ccgcattacc tgatgggcgt
    10441 aggcacgccg gaagacctcg tgtacggcgt ggcacacggc atcgatatgt tcgactgcgt
    10501 gatgcccacc cgcaatgcac gcaacggctg gctgtttacc cgtttcggcg atttgaaaat
    10561 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
    10621 ctgccaaaac ttcagccgcg cctacctgca ccatctgcac cgcaccggcg aaatcttggg
    10681 cgcacagttg aacaccatcc acaatctgca tttctaccaa gtcattatgg cggaaatgcg
    10741 cgaagccgtc gaacaaggca aatttgccga ctggcaggcg cgattccacg aaaaccgcgc
    10801 acgcggcaca gactgattcc ctgcaatgcc gtctgaagcc gcttcagacg gcattttgcc
    10861 gctttaccct acccgcacta tttttcagaa accgatcatg cttccattct ttccagaacc
    10921 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacgcc
    10981 cgacgcgccg accgcgcagg cggtacgacc ttatctgaag tcctttctga ccgaccggcg
    11041 cgtcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggtctgg tactgacgct
    11101 ccgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgccctt
    11161 agaagtttac acagcacgcc aagctgccgc gcttgccaaa cgtatgcccg acctcatcgt
    11221 ccgccacgcc atgacctacg gcaatccttc ggtggcagac gtattgtcgg aactgaaggc
    11281 gcaaggcgcg ggcagactgc tggtcatccc gatgtacccg caatatgccg cttccagcag
    11341 cggagcggca gtagataagg tgtgcgaaca attactgctg caacggaacc aaatgagcgt
    11401 ccgcaccgtt tcgcgctttt atgacgatac cggctacatt gatgcgatga aaaaccacat
    11461 cctccgatat tgggcggaac acgggcgcgg gaaaaaactg atgttgagtt ttcacggcgt
    11521 accgcagaag caccacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
    11581 actgcttgcc gaagcactgg aactgaccga agaccaatat gtcgtatcgt tccaaagcca
    11641 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
    11701 acagggggta accgagctgg acgtattttg cccgggcttt ttggcagact gtttggaaac
    11761 catggaagaa atcgccctga tggggcggga acagttttat gaagcaggcg gcaaaagcta
    11821 ccgctacatc ccctgcctca acgacaaccc cgactggata gatgcactcg tcgcacttgc
    11881 cgaagaaaac cttggcagct ggcgttgaaa ctgtttgacc catgtctaaa atgccgtctg
    11941 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgctgga
    12001 caacaacatc aagtgcaacc ttatagtgga ttaaatttaa accagtacgg cgttgcctcg
    12061 ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tatcctgatt tttgttaatc
    12121 cactatacta ctgattcggc aaaaaaatct ttcacccgct acgcaaaatc aacaacaagg
    12181 tttcaaacat gaaggaaaca ccaatgaaca caacccgact gccgaccgcc ctcgtcttgg
    12241 gctgcttctg cgccgccgct tctgccgccg acaacagcat catgacaaaa gggcaaaaag
    12301 tgtacgaatc caactgcgtc gcctgccacg gcaaaaaggg cgaaggccgc ggaaccatgt
    12361 ttccgccgct ctaccgctcc gacttcatca tgaaaaaacc gcaggtgctg ctgcacagca
    12421 tggtcaaagg catcaacggt acaatcaaag tcaacggcaa aacctacaac ggattcatgc
    12481 ccgcaaccgc catcagcgat gcggacattg ccgccgtcgc cacttatatc atgaacgcct
    12541 ttgacaacgg cggcggaagc gttaccgaaa aagacgtaaa acaggcaaaa agcaaaaaaa
    12601 actaaacaga caaaatgccg tctgaaaccg gcaatccggc ttcagacggc attcaaatca
    12661 aacctttcta acgcagcact ttcagcccca tagacccgat attgccgcca ccgtcaacct
    12721 cctttgcggt cagcataaag ccgccgatgt caatacggcc gccctcgacc ggctcctgac
    12781 tatcggaacg cttatcgaaa agctcggcaa ggctcaaacc ctcttcgccc gcttccagcc
    12841 tcaaaccgta agtaagtgcc aaatcacccg aacgtgctgc cggcgaaacg acaaactcac
    12901 cgaagaaatc aaaatgctca cggacgctaa tacccgtctc ggtaaagtat ttcgccattt
    12961 tatagtgaa
//
LOCUS       DAWHRS010000049        12923 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWHRS010000049 DAWHRS010000000
VERSION     DAWHRS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12923
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.049"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            44..457
                     /locus_tag="WIB82_000961"
     CDS             44..457
                     /locus_tag="WIB82_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ3842277.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            503..1132
                     /gene="nth"
                     /locus_tag="WIB82_000962"
     CDS             503..1132
                     /gene="nth"
                     /locus_tag="WIB82_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ3842278.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1270..2769
                     /locus_tag="WIB82_000963"
     CDS             1270..2769
                     /locus_tag="WIB82_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ3842279.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            2971..4491
                     /locus_tag="WIB82_000964"
     CDS             2971..4491
                     /locus_tag="WIB82_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ3842280.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4548..5633
                     /gene="nagZ"
                     /locus_tag="WIB82_000965"
     CDS             4548..5633
                     /gene="nagZ"
                     /locus_tag="WIB82_000965"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ3842281.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEERQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGQCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5861..5937
                     /locus_tag="WIB82_000966"
     tRNA            5861..5937
                     /locus_tag="WIB82_000966"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5895..5897,aa:Pro,seq:cgg)
     gene            complement(6272..6910)
                     /locus_tag="WIB82_000967"
     CDS             complement(6272..6910)
                     /locus_tag="WIB82_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ3842282.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQNGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(6907..7278)
                     /locus_tag="WIB82_000968"
     CDS             complement(6907..7278)
                     /locus_tag="WIB82_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ3842283.1"
                     /translation="MKLLSTLLVLLVAAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(7547..7969)
                     /gene="queD"
                     /locus_tag="WIB82_000969"
     CDS             complement(7547..7969)
                     /gene="queD"
                     /locus_tag="WIB82_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ3842284.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDLVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(7976..8494)
                     /locus_tag="WIB82_000970"
     CDS             complement(7976..8494)
                     /locus_tag="WIB82_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ3842285.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(8522..9181)
                     /gene="queC"
                     /locus_tag="WIB82_000971"
     CDS             complement(8522..9181)
                     /gene="queC"
                     /locus_tag="WIB82_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ3842286.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERASWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            9286..9711
                     /locus_tag="WIB82_000972"
     CDS             9286..9711
                     /locus_tag="WIB82_000972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842287.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSECGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            9726..10952
                     /locus_tag="WIB82_000973"
     CDS             9726..10952
                     /locus_tag="WIB82_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ3842288.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(10973..11254)
                     /locus_tag="WIB82_000974"
     CDS             complement(10973..11254)
                     /locus_tag="WIB82_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3842289.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(11333..11467)
                     /locus_tag="WIB82_000975"
                     /pseudo
     CDS             complement(11333..11467)
                     /locus_tag="WIB82_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(11466..11924)
                     /locus_tag="WIB82_000976"
     CDS             complement(11466..11924)
                     /locus_tag="WIB82_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842290.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     SYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(12492..12836)
                     /locus_tag="WIB82_000977"
     CDS             complement(12492..12836)
                     /locus_tag="WIB82_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842291.1"
                     /translation="MRTHFVISNVPSTPAPLRGRKLTGKLILEVPAQVNPIPQSVLRA
                     AQEENVIIRDTTGRIYNRWWFSIRWLCRCTSCKKRIIDTNRENRGRCVSNCKCKHWIS
                     MDCQFVKAKRRS"
BASE COUNT         3029 a         3459 c         3243 g         3192 t
ORIGIN      
        1 gtacaatccg tcgccgattt ttgcctgttt gagactgttt gcaatgatgc ccgcctacgc
       61 ctacgccgcg ctcgcctttt ccgccttcac ttccgccacc ctcctgcccg gcacatccga
      121 agccgccttt gccctgttcg tccgccgctt tcccgaacac gcatacggcg cgttgctgtg
      181 cgccggcctt gccaacggat tgggcagtat ggtttcctat tggatgggac gtttgctgcc
      241 ttcccgaaaa atgccgtctg aaaaaacact gaatctgatg cggcgtttcg gtatttggct
      301 gcttccgttt gcctggctgc ccgtcgtcgg cgacgcactg ccgcttgccg ccggctggct
      361 gcggttgaat ccgtggacaa gcgggctgat gctggttatc ggcaaaacgg cgcgttacgc
      421 cttcattctg tggggaatgc aatattacgc cgcctgaacc gtgcgttata atgccgaccg
      481 cacaaaccga ctgcacaaaa aaatgaacag acacatccgc caagaaattt tcgaacgctt
      541 ccgcgccgcc aacccccatc cgaccaccga gctgaatttc aactcccctt tcgagctttt
      601 aatcgccgtt ctgctttcag cgcaggcgac cgatgtcggc gtaaacaagg cgacggcgaa
      661 gctgtttccg gttgccgata cgccgcaggc aatgctggat ttgggcttgg acggcgtgat
      721 ggaatacacg aaaaccatcg ggttgtataa aaccaagtcc aagcacatca tgcagacctg
      781 ccgcatcctg ctggaaaaat acaagggcga agtgccggaa gaccgcgagg ctttggaatc
      841 gttgccgggc gtggggcgca aaacggcaaa cgtggtattg aacacagcgt tcggacaccc
      901 cgtcatggcg gtcgatacgc atattttccg cgtgtccaac cgaaccaaaa tcgcacccgg
      961 gaaagatgtg cgcgaagtcg aagacaaact gatgcgcttc attcctaaag aatttctgat
     1021 ggacgcgcac cactggctga ttttgcacgg acgctacacc tgcaaggcac tcaaaccgca
     1081 atgccaaacc tgcatcatca acgatttgtg cgaatatccc gccaaagcct gactgccgcc
     1141 gcccttcaaa atgaaacgct tgaaaattcg gacggacgtt gtagaatttc aacctatgcc
     1201 gtctgaagcc gacaaaatgc gcttcagacg gcattaaagc cggtaccggc atccgacaaa
     1261 ggacaaaacg tgttcaaaaa ataccaatac ctcgctttgg cagcactgtg tgccgcctcg
     1321 ctggcaggct gcgacaaagc cggcagcttt ttcggtgcgg acaaaaaaga agcatccttc
     1381 gtagaacgca tcaaacacac caaagacgac ggcagcgtca gtatgctgct gcccgacttt
     1441 gtccaactgg ttcaaagcga aggcccggca gtcgtcaata ttcaggcagc ccccgccccg
     1501 cgcacccaaa acggcagcag caatgccgaa accgattccg acccgcttgc cgacagcgac
     1561 ccgttctacg aatttttcaa acgcctcgtc ccgaacatgc ccgaaatccc ccaagaagaa
     1621 gcagatgacg gcggattgaa cttcggttcg ggcttcatca tcagcaaaga cggctatatt
     1681 ctgaccaata cgcacgtcgt taccggcatg ggcagtatca aagtcctgct caacgacaag
     1741 cgcgaatata ccgccaaact catcggttcg gatgtccaat ccgatgtcgc ccttctgaaa
     1801 atcgacgcaa cggaagagct gcccgtcgtc aaaatcggca atcccaaaga tttgaaacca
     1861 ggcgaatggg tcgctgccat cggcgcgccc ttcggcttcg acaacagcgt gaccgccggc
     1921 atcgtgtccg ccaaaggcag aagcctgccc aacgaaagct acacaccttt catccaaacc
     1981 gacgttgcca tcaatccggg caactccggc ggcccgctgt tcaacttaaa aggacaggtc
     2041 gtcggcatca actcgcaaat atacagccgc agcggcggat tcatgggcat ttccttcgcc
     2101 atcccgattg acgttgccat gaatgtcgcc gaacagctga aaaacaccgg caaagtccaa
     2161 cgcggacaac tgggcgtgat tattcaagaa gtatcctacg gtttggcaca atcgttcggt
     2221 ttggacaaag ccggcggcgc actgattgcc aaaatcctgc ccggcagccc cgcagaacgt
     2281 gccggcctgc aggcgggcga catcgtcctc agcctcgacg gcggagaaat acgttcttcc
     2341 ggcgaccttc ccgttatggt cggcgccatt acgccgggaa aagaagtcag cctcggcgta
     2401 tggcgcaaag gcgaagaaat cacaatcaaa gtcaagctgg gcaacgccgc cgagcatatc
     2461 ggcgcatcat ccaaaacaga tgaagccccc tacaccgaac agcaatccgg tacgttctcg
     2521 gtcgaatccg caggcattac ccttcagaca cataccgaca gcagcggcgg acacctcgtc
     2581 gtcgtacggg tttccgacgc ggcagaacgc gcaggcttga ggcgcggcga cgaaattctt
     2641 gccgtcgggc aagtccccgt caatgacgaa gccggtttcc gcaaagctat ggacaaggca
     2701 ggcaaaaacg tccccctgct gatcatgcgc cgtggcaaca cgctgtttat cgcattaaac
     2761 ctgcaataac acacataatt taaccaaaat attttttaaa tgataaaatg cccgtcaata
     2821 ccgattaacg ggcattttac aaaaatcagt tggttgattt taaacaaata ttttttacga
     2881 caaaaaatat ttccgcccat tttgccaaca aaacccaaac cggaggcgga cagatacgct
     2941 cccatccgga cgcttaacac ccccataacc atgcagctga tcgactattc acattcattt
     3001 ttctcggttg tgccaccctt tttggcactg gcacttgccg tcattacccg ccgcgtactg
     3061 ctgtctttag gcatcggtat tctggtcggc gttgcctttt tggtcggcgg caaccccgtc
     3121 gacggtctga cacacctgaa agacatggtc gtcggcttgg cttggtcaga cggcgattgg
     3181 tcgctgggca aaccaaaaat cttggttttc ctgatacttt tgggtatttt tacttccctg
     3241 ctgacctact ccggcagcaa tcaggcgttt gccgactggg caaaacggca cattaaaaac
     3301 cggcgcggcg cgaaaatgct gaccgcctgc ctcgtgttcg taacctttat cgacgactat
     3361 ttccacagtc tcgccgtcgg tgcgattgcc cgccccgtta ccgacaagtt taaagtttcc
     3421 cgcgccaaac tcgcctacat cctcgactcc actgccgcgc ctatgtgcgt gctgatgccc
     3481 gtttcaagct ggggcgcgtc gattatcgcc acgcttgccg gactgctcgt tacctacaaa
     3541 atcaccgaat acacgccgat ggggacgttt gtcgccatga gcctgatgaa ctattacgca
     3601 ctgtttgccc tgattatggt gttcgtcgtc gcatggtttt ccttcgacat cggctcgatg
     3661 gcacgtttcg aacaagccgc gttgaacgaa gcccacgatg aaactgccgt ttcagacgct
     3721 accaaaggtc gtgtttacgc actgattatt cccgttttgg ccttaatcgc ctcaacggtt
     3781 tccgccatga tctacaccgg cgcgcaggca agcgaaacct tcagcatttt gggggcattt
     3841 gaaaacacgg acgtaaacac ttcgctggta ttcggcggca cttgcggcgt ccttgccgtc
     3901 gttctctgca cgctcggcac gattaaaacc gccgactatc ccaaagccgt ttggcagggt
     3961 gcgaaatcta tgttcggcgc aatcgccatt ttaatcctcg cttggctcat cagtacggtt
     4021 gtcggcgaaa tgcacaccgg cgattacctc tccacactgg ttgcgggcaa catccatccc
     4081 ggcttcctgc ccgtcatcct cttcctgctc gccagcgtga tggcgtttgc cacaggcaca
     4141 agctggggga cgttcggcat tatgctgccg attgccgccg ccatggcggt caaagtcgaa
     4201 cccgcgctga ttatcccgtg tatgtccgca gtaatggcgg gggcggtatg cggcgaccac
     4261 tgctcgccca tttccgacac gaccatcctg tcgtccaccg gcgcgcgctg caaccacatc
     4321 gaccacgtta cctcgcaact gccttacgcc ttaaccgttg ccgccgccgc cgcatcgggc
     4381 tacctcgcat tgggtctgac aaaatccgcg ctgttgggct ttggcacgac aggcattgta
     4441 ttggcggtgc tgatttttct gttgaaagat aaaaaacgcg ccaacgcctg acccctttcc
     4501 ctgttcagac ggcatatgcc gtctgaagct ttttggagca aaccgcaatg accgtccccc
     4561 atattccgcg cggccccgtc atggcagaca ttgccgcctt ccgcctgacc ggagaagaaa
     4621 ggcaacgcct gctcgatccc gccgtaggcg gcatcatcct cttccgccgc aacttccaaa
     4681 acatcgaaca actcaaaaca ctcaccgccg aaatcaaagc cctgcgcaca cccgaactca
     4741 ttatcgccgt cgatcacgaa ggcggcaggg tgcaacgttt catcgaaggc ttcacccgcc
     4801 tgcccgcaat gaacgtttta gggcaaattt gggacaaaga cggcgcgtcc gccgccgaaa
     4861 ccgccgccgg acaagtcggc cgggtgttgg caaccgagct ttccgcctgc ggcatagacc
     4921 tctccttcac ccccgttctc gacttggact ggggacaatg cgccgtcatc ggcaaccgca
     4981 gcttccaccg caaccccgaa gcggtcgcac gcctcgccct cgcccttcaa aaaggtttgg
     5041 caaaaggcgg tatgaaatcc tgcggcaaac attttcccgg acacggattt gtcgaaggag
     5101 acagccatct ggtcttgccg gaagacaggc gcagcctgtc cgaactcgaa gccgccgacc
     5161 tcgccccctt ccacattatg agccgcgaag gtatggcggc ggttatgccc gcccacgtcg
     5221 tttatccaca agtggacaca aagcccgcag ggttttccga aatctggctc aaacaaattt
     5281 tgcgccgcga catcgggttc aaaggcgtga ttttctcgga cgatttaact atggagggcg
     5341 cgtgcggggc aggcggcatc aaagaacgcg cccgcatttc ctttgaggca ggttgcgaca
     5401 tcgtcctcgt ctgcaaccgt cccgaccttg tggacgaact gcgcgatgat ttccgcattc
     5461 ctgacaatcc cgctttggcg caacgttggc aatacatggc caacacgctc ggcagtgccg
     5521 ccgcgcaagc cgttatgcag acggcggatt tccaagcggc gcaagccttt gttgccggcc
     5581 ttgcctcgcc gcaagacacg gcgggcggcg taaaagtcgg cgaagccttt taaaccggct
     5641 gcgtttcaac aatctgccga tttcatcaac cgtttgtcag ggaaaggcaa tgattttttc
     5701 ggtcaggtag aaagttatct tattgaatta cttaaagata atgatgatta tcaacactat
     5761 attcaaacac actcctttaa atccgcccga cacgcggcaa aaaggcaaaa cggtataggc
     5821 acgccgtatt ttttgcgcta taatcacgcc ttacttaatt cggaatgtag cgcagcccgg
     5881 tagcgcactt cgttcgggac gaaggggtcg gaggttcgaa tcctctcatt ccgaccaaat
     5941 ccaaaccgcc tcggcataga ttgccggagc ggtttttcct tttggcggca acagaaaaat
     6001 gccgtctgaa acagaatgct gtttcagacg gcatttcgtt ttccctaacc ctgccatttg
     6061 cgcgcactgg cacagagtat cggcaaggtt caaaagacca tcggaattta ggaagccgag
     6121 cttgtaccca acggcagcat cttattcagt aaggctccgt cctgattggg cggtggaaat
     6181 gccggcattt ccacgcagaa gcggcacact ttccccgtat gtcaaacatc ggtaaaaccg
     6241 tacgccgtcc ctttgagtta cactgcttaa actactctat tcccgcccat ttgtgcgtct
     6301 gcacgctcaa ctgccaatgc accggcgcgt cggggcggct gttcagaata ccgatttggc
     6361 ggatggtgtc gtagatgttc attgcaccgt tttgttcgca gggcgaaagg tagtaatgat
     6421 gcgcacggat tttgcgttcc atattttcac agaacgcaac gacatcgccg tcggcaacaa
     6481 tccgcacttc gtcggctgtt tcgatacagc ttttttcgta tttggcggca tagcaggctt
     6541 tggggctggt ggcgacatag tcgatttgcg gcggcgcggg attgagtccg ttggtttcga
     6601 tacagaggaa ataaccttcc gccttgagca tgtccagcag catatcgaga tgcggctgga
     6661 tggtcggttc gccgccggtg atgatgatgt tgcgggcggc ataggctttc agacagccta
     6721 agatgtcgga caggctcatc atgccgaatg tcaaataatc ggtatcacac cagccgcagg
     6781 cgaggttgca tttgcccagg cggacgaaca cggcgggcat gcccgtgttc cagccctcgc
     6841 cttgcaggct ttcaaagatt tcgacgatgc ggtattgggg attttcgggg gcgacgctga
     6901 tttttttcat accgccaaca ccgccgccgc tgccaaaaat gcgggcaaac cttgtttgac
     6961 gagtatgcct ttgttgccgg aagagaacgc gccccacgcg gcggcaatca ggacgaagcc
     7021 aagaaacagt accgtcgcgc cgtacaccgc gttatccggc gcggcaaacc gcgaccacac
     7081 cagcccgatg ccgagaaagc cgttatacag cccttggttg ccgaacaggg tctgcacgcg
     7141 attttgttcc ataaattcat aaggcagctt gaatatttcc gccgcttttt cgctgggaat
     7201 ctgcgtcatt tcaagccagg cgatgtagaa atgttcggcg gcgacaagga ggactaagag
     7261 ggtggagaga agtttcatgg gtattccttt taatgggtgt atttgatatg gaaggtcgtc
     7321 tgaaaaaacg gggaatccgt acaggagaaa aaaccgtttt acggtaaatt caaataagga
     7381 cgctctaaac cgtcctttcc gcgcaggcgg gaatccaaag atttggaatt gctgcaattt
     7441 ttaaatattc ctgaaaaacc aaggtctgga ttcccgcctg cgcgggaatg acgcggttgg
     7501 gcatgtctta ttggaattta ctatatcgat acgttcaaga tattccctac tccccttcat
     7561 actccgcaca cgatgtcggc gtttcccaca atttgacgcg gcacacgttc agccccgcgt
     7621 ttttcagacg gtcgtacatt tcgaccgcca tattttcggc agtggtgcgg cagggcaggc
     7681 gcagggtttt catgttccag ccttccaaga gtgcggcgat ttgagattcg cgcccgttgc
     7741 cgccgtggta gatgaaggcg tggtcgaagg ggtcggtaat gtgttgtttg acaatggctt
     7801 tcaagtcggt aaagtccatc accataccgt ctttcggccc gcctctgacg aggtcgtctg
     7861 aaacggtgat ttcgagtttg taggtatgcc cgtgcaggtt ttggcatttg ccgtcatgcc
     7921 cgtcgagcat atgcgaggag tcgaaggtga agattttggt gattttcatg ggttctcaaa
     7981 tgggcaaata gccttgaaag ccgatttcgg cgggtttgcc gtgcggattc ggggtcaggc
     8041 ggatatggaa attgccgatg cggtcgagcg caagcaggtt gtccacgtct tttaagttgt
     8101 ctttatgttg ctgaacatag ctgtcggtca acagggtttg cagggctttt tgtttggctt
     8161 cggcaggcgt ggcggcggcg aacagcccaa aagcgtgcgc ttcggcgagc tgccctgcgc
     8221 gataaccgcc gacattggcg aaatacaggc gcggggcgtg ttcagacggc attttcgttt
     8281 tgggcgtttc ggatacggcg atgtcgtaac cgtccgccca ttcgacaacc tgccagccgt
     8341 cgatgtggat tttgtccgca tcgccgaacc acgcggcttt gagtgcgggg acggcttcac
     8401 ggtagtcgtc gcacacggca aattggatgt cgtgcacttc gatattcgac ctgccggcat
     8461 tgccgccgag gtaaaacata tggagcttgg gcatatcgta ttccttatgg tttgcgcgtg
     8521 ttcagacggc ctttttactt tccagatatt ccgccagccc gcgttcgcgc aagatacagc
     8581 tcgggcattc gcggcagccg ccgacgatgc cgttatagca ggtgtgggtt tgctcgcgga
     8641 tatagtccag cgcgcccatt tcgtccgcca acgcccaagt ttgcgccttg gtcagataca
     8701 tcagcggcgt gtggatttgg aaatcatagt ccatcgccag attaagggta acgttcatgg
     8761 atttgacgaa cacgtcgcgg cagtcaggat agccggagaa gtcggtttcg cacacgcccg
     8821 cgatgatgtg ccgtatcccc tgccctttgg cgtaaatcgc ggcatagagc aggaaaagcg
     8881 cgttgcggcc gtctacaaag gtattcggaa cgccgttttc ggcagtttcg atggcggcgg
     8941 tgtcgtccat cagggcattg tgcgtaatct gccgcatcag gctcaagtcg agtacggttt
     9001 gtttgacgcc caaatcctgc gcaatccagc tggcacgttc cagctcgacg gcatggcgtt
     9061 gcccgtattg gaaagtaatg gcttggacgt tttcgcgccc gtagatttgg attgcctgaa
     9121 tcaggcaggt ggtcgaatcc tgaccgcccg aaaagatgac caaggctttt tggtttgaca
     9181 tatcgaatcc tagttttggt aacgcgggag gtttgcgaac cgcgaagttg gacagtaaag
     9241 acgcggatta tacaggcaaa cgcgttcaga cggcatctga acgccatgcc gtctgaacac
     9301 gcggcttatg ccgacttgac ggcgagttcc gccaagcgtt tgctttgtgc gaaggcgata
     9361 ttgttttctt cggcggtcag gacgggagcc gctgtcggga atatcttttt tgacggcttc
     9421 gcagacggcg gagactttgg ggacggtgcg caatacggct tcgcaacctt caacgctgtc
     9481 gatgccgcga gcgattcgga gtgcgggatt gcgggtgctg ccgttttgga aatagtagag
     9541 gacgaggatt ttgaggggga tttgggttca tgggcggatt ccgttaaaat ggttcggtct
     9601 tcagacggca taacagttgt tccgatgccg tctgaaacag ccgcagttca ggcatttgtt
     9661 cctgacggca aaattggcta taacatcggg catcaatgcc caacttactg accgggcaag
     9721 aaattatgac ctttttacaa cgtttgcaag gtttggcaga caataaaatc tgtgcgtttg
     9781 catggttcgt cgtccgccgc tttgatgaag aacgcgtacc gcaggcggcg gcaagcatga
     9841 cgtttacgac gctgctggca ctcgtccccg tgctgaccgt gatggtggcg gtcgcttcga
     9901 ttttccccgt gttcgaccgc tggtcggatt cgttcgtctc cttcgtcaac caaaccattg
     9961 tgccgcaggg cgcggacatg gtgttcgact atatcaatgc gttccgcgag caggcgaacc
    10021 ggctgacggc aatcggcagc gtgatgctgg tcgttacctc gctgatgctg attcggacga
    10081 tagacaatac gttcaaccgc atctggcggg tcaattccca gcgtccgtgg atgatgcagt
    10141 ttctcgtcta ttgggcttta ctgacgttcg ggccgctgtc tttgggcgtg ggcatttcct
    10201 ttatggtcgg ctcggtacag gatgccgcgc ttgcctcagg tgcgccgcag tggtcgggcg
    10261 cgttgcgaac ggcggcgacg ctgaccttca tgacgctttt gctgtggggg ctgtaccgct
    10321 tcgtgccaaa ccgcttcgtt cccgcgcggc aggcgtttgt cggggctttg gcaacagcgt
    10381 tttgtctgga aaccgcgcgc tccctcttca cttggtatat gggcaatttc gacggctacc
    10441 gctcgattta cggcgcgttt gccgccgtgc cgttttttct gttgtggctg aacctgttgt
    10501 ggacgctggt cttgggcggc gcggtgctga cttcttcact ctcctactgg cagggagaag
    10561 cgttccgcag gggcttcgac tcgcgcggac ggtttgacga cgtgttgaaa atcctgctgc
    10621 ttctggatgc ggcgcaaaaa gaaggcaaag ccttgcctgt tcaggagttc agacggcata
    10681 tcaatatggg ctacgacgag ttgggcgagc ttttggaaaa gctggcgcgg cacggctaca
    10741 tctattccgg cagacagggt tgggtgttga aaacgggggc ggattcgatt gagttgaacg
    10801 aactcttcaa gctcttcgtt taccgtccgt tgcctgtgga aagggatcat gtgaaccaag
    10861 ctgtcgatgc ggtaatgatg ccgtgtttgc agactttgaa catgacgctg gcagagtttg
    10921 acgctcaggc gaaaaaacag cagcaatctt gagtattttt gaaactgtat ttttatccta
    10981 aagacatttt cctatctgct gattccaccg tttcctttct gtgtgccgcc ataattttta
    11041 tgatagacaa gccattcaag tttgcattga ctgctttttc attggcaata tccaaatggc
    11101 tggtcgcttc atctaaaaat aggattttcg gttcgcaata taaggcccgc gccaatacga
    11161 tgcgttgttt ttgtccgcct gacagtgcgc tgcccatatc gccgattaag gtttcatagc
    11221 ccataggcat ggcggagatt tcttcgtgaa tcatagcaag gcaggcgcaa tgttcgattt
    11281 ttcctctatc cggttccgta tcgaaaaaac aaggggctgt actagattag ccctaaatcc
    11341 cacaccaatc ccgcaggatt ttaagctgtt gagacggtgt gccgaagtta aatcgaaatt
    11401 cgcattcttt caagaacagc gggaaagatt tgcaatcgat tccgttgtat tttcgcaaga
    11461 cacgtttagt ctagagtctg tatattacat ttatttttag ggtctgctag ccaatttctt
    11521 gttcccttca ttattttatc ttctgaaaga aaattatttt tttccatgct attaatatta
    11581 atgatatgat ttttatttaa aataattttt ccatatgaaa tttccggttt gtaatagcaa
    11641 gagtctgaag caaatccttg cccgtcaata taagaaaaat aaatgttatc atcctcctta
    11701 taatattcta aaaccaatcc ggatattatt ttatcaaaat tcccttcatt tccatctttc
    11761 atgactaaaa tataattagc aggccaacct ttatcgtaaa catattcaaa ctgatagttc
    11821 ccgaaactct cgatatccga actaaaaaag aagaaaagca aattaatagc caatacaaaa
    11881 acaaataaga acaaaaatag caaaattttc aacttagtta acaatattta cctctccttt
    11941 aaattcaatc ctgaaaggta ccccttaccc tgggctacca attatagttt ccatatttct
    12001 aaatattgtt tttacattac ttttttctcc ccccaaagga atgcatttta aaatcatgct
    12061 tttcaggtgc taatcgatac ttaccattaa catctttaat cacagatata tttccatgta
    12121 tagcacaacg tgaaaaatct gagtattata tacagttata ccttactgaa acaaagctag
    12181 ctgtacatat ctgtaccata tttttactac aaacaaaaaa tatttataat tattaaggat
    12241 aagataaaaa cccccaacgc cagattaatt cttaataatt tcaaataaaa aatatttgag
    12301 attttttctt ttccaaataa tatattgaaa tatttaaagt taaggtagct tgatttaatt
    12361 agaaaatcaa aaaaaccatt aattaatttt tcttctttaa atattttata tgatattata
    12421 aaattaaata acattgcgaa aacatatgaa atcatcaaaa taccaaatat atagtgggaa
    12481 tgcattatta tttagctcct tcttttggct ttgacaaatt gacagtccat tgatatccaa
    12541 tgcttgcatt tgcaattgct gacgcacctg cccctgtttt ccctgttagt atcaatgatt
    12601 cttttcttgc aagatgtgca cctgcacaac cacctaatac taaaccacca cctattgtaa
    12661 atccttcctg ttgtatctct aatgataaca ttttcttctt gtgccgccct taatacagat
    12721 tgtggaattg gattgacctg agcaggaact tctaaaataa gttttcctgt aagttttctt
    12781 cctcttagcg gagcaggtgt acttggaaca ttggaaatga caaagtgagt acgcaaacca
    12841 cctgaaggcg atgtgtttga acctgagacc tttgcaaaaa tagtctgtta acgaaatttg
    12901 acgcataaaa atgcgccaaa aaa
//
LOCUS       DAWHRS010000050        11886 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWHRS010000050 DAWHRS010000000
VERSION     DAWHRS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.050"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1005
                     /gene="rfbB"
                     /locus_tag="WIB82_000978"
     CDS             <1..1005
                     /gene="rfbB"
                     /locus_tag="WIB82_000978"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ3842292.1"
                     /translation="IRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFEQVDICDRAE
                     LDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARAYWQQMPSEQ
                     HEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVRAWLRTYGLP
                     TIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVEDHARALYQV
                     VTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYEDLITFVQDRPGH
                     DVRYAIDTAKISRELGWKPQETFESGLHKTVQWYLDNKTWWQNVLNGSYRLERLGTGK
                     "
     gene            1068..1934
                     /gene="rfbA"
                     /locus_tag="WIB82_000979"
     CDS             1068..1934
                     /gene="rfbA"
                     /locus_tag="WIB82_000979"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ3842293.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKCLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            1979..2521
                     /gene="rfbC"
                     /locus_tag="WIB82_000980"
     CDS             1979..2521
                     /gene="rfbC"
                     /locus_tag="WIB82_000980"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ3842294.1"
                     /translation="MNIIDTALPDVKILKPQVFTDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWTGATLS
                     AQNQYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPKAEHSLIWNDPTVGIDWPLQGE
                     PNLSPKDLAGKVLAEAVTFG"
     gene            2577..3698
                     /locus_tag="WIB82_000981"
     CDS             2577..3698
                     /locus_tag="WIB82_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide phosphotransferase"
                     /protein_id="HEZ3842295.1"
                     /translation="MNLNKIRKFFRESHIFFRDFLNKRFPPYNVEQPIPETEEFTLIN
                     ADANLASLTENVLPNFPIDVVFTWVDNTDKAWQEQYYRTLQPIDQEDIGLYATDPARF
                     SNHNELFYSVQAVQKFMPWVRNIFIVTADQKPKWLDENIHSKIKLINHSQLIDAKYLP
                     TFNSHVIEANLYKIPDLSEHFIYFNDDVFVARPLMPNHFFENNGLASLFVANKSFQKM
                     RQRGLITPTLTASEHALKLLKRHYPTININTPLVHTYVPLRKTSFQKAWSLFEDEINS
                     FLNNKVRHNSELNMASFLIPWLMYLDGYATPKREICYYFNIRSSHAQTQYKKLLFEKE
                     HLHMPHSFCINDSSSNNADKNYALHFRNFMDTYFEIETE"
     gene            3743..5767
                     /locus_tag="WIB82_000982"
     CDS             3743..5767
                     /locus_tag="WIB82_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ3842296.1"
                     /translation="MIFSTFGLFKQRLLLTKFLDKISTDKTALFWGKKHPKRRKLAHI
                     LRLSLLNLEDGFLRSLGLGVAGYPPYSIVYDDIGIYYDTTRPSRLEQLILAADTMPSE
                     TLAQARQAMDFILQHHLSKYNHAPELSDDHPLRSPSKPETVLIIDQTFGDMAIQYGGA
                     DASTFELMFQTALNENPQADIWVKTHPDVLCGKKQGYLTQLAQQHRVHLLAEDINPIS
                     LLQNVDKVYCVTSQMGFEALLCGKPLTTFGLPWYAGWGVSDDRHPKIDSLVQTQRRAP
                     RNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELYCVGMSLWK
                     RAVAKPFFNVPSCRLKFISSTQKLAGVKLSDDARILAWGNGKEAIVRFAEQHHIPLLR
                     MEDGFIRSVGLGSNLVPPLSLVTDDMSIYFNAEAPSRLEHILQNQNFDDQDFQTALKL
                     QKMLTENHISKYNVGNSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYHNLDLLRTV
                     RERHPDAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQYADEIHTMT
                     SLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGTLIHYPDYI
                     HPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK"
     gene            5904..7163
                     /locus_tag="WIB82_000983"
     CDS             5904..7163
                     /locus_tag="WIB82_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ3842297.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKNTSDSSTPTT"
     gene            7532..8938
                     /locus_tag="WIB82_000984"
     CDS             7532..8938
                     /locus_tag="WIB82_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ3842298.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            9156..10370
                     /gene="gltS"
                     /locus_tag="WIB82_000985"
     CDS             9156..10370
                     /gene="gltS"
                     /locus_tag="WIB82_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ3842299.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     IAAIVLFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            10469..11485
                     /locus_tag="WIB82_000986"
     CDS             10469..11485
                     /locus_tag="WIB82_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ3842300.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            11496..11693
                     /locus_tag="WIB82_000987"
     CDS             11496..11693
                     /locus_tag="WIB82_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842301.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            11711..11860
                     /locus_tag="WIB82_000988"
     CDS             11711..11860
                     /locus_tag="WIB82_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842302.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
BASE COUNT         3163 a         3344 c         2668 g         2711 t
ORIGIN      
        1 atccgaaaca cccaagattc cgtcgtcaac ctcgacaaac tgacctacgc gggcaatctc
       61 gaatcgctga ccgacattgc cgataatccc cgctatgctt tcgagcaagt ggacatttgc
      121 gaccgcgccg aactcgaccg cgtattcgca caacaccggc ccgatgccgt gatgcacttg
      181 gcggcggaaa gccatgtcga ccgctctatc ggttcggcag gcgagtttat ccaaaccaat
      241 atcgtcggca cattcaatct gcttgaagca gcccgcgcct actggcaaca aatgccgtct
      301 gaacagcacg aagccttccg tttccaccat atttccaccg atgaagtcta tggcgattta
      361 ggcggcacgg acgatttgtt taccgaaacc gcgccctacg cgccgtccag cccctactct
      421 gcctctaaag cgtccagcga ccacctcgtc cgcgcgtggt tgcgtactta cggcttgccg
      481 accattgtaa ccaactgctc caacaactac ggtccttacc attttccgga aaaactcatt
      541 cctttgatga ttctgaacgc gcttgacggc aaaccgctgc ctgtgtacgg cgacggtatg
      601 caaatccgcg actggctgtt tgtcgaagac cacgcgcgcg cactgtatca ggttgttacc
      661 gaaggtgttg tcggcgaaac ctacaatatc ggcggccaca atgaaaaagc caatattgaa
      721 gtcgtcaaaa ccatctgcgc cctgctggaa gaactcgctc ccgaaaaacc ggccggtgtg
      781 gcgcgttatg aagatttgat tactttcgta caagaccgcc ccggccatga cgtacgctac
      841 gccattgata cagccaaaat cagtagggaa ctaggctgga aaccgcagga aaccttcgag
      901 tccggcctcc acaaaaccgt gcaatggtat ctggacaaca aaacctggtg gcaaaacgta
      961 ttgaacggca gctatcgttt ggaacgctta ggtacaggca aatagttttc agacggcatc
     1021 ccgacgcaat gccgtctgaa aacccatcgc aaaggaagaa agaaaagatg aaaggcatca
     1081 tactggcagg cggcagcggc acgcgcctct accccatcac gcgcggcgta tccaaacagc
     1141 tcctgcccgt gtacgacaaa ccgatgattt attacccctt gtcggttttg atgctggcgg
     1201 gaatccgcga tattttggtg attaccgcgc ctgaagacaa cgcctctttc aaatgcctgc
     1261 ttggcgacgg cagcgatttc ggcatttcca tcagttatgc cgtgcaaccc agtccggacg
     1321 gcttggcaca ggcatttatc atcggcgaag aatttatcgg caacgacaat gtttgcttgg
     1381 ttttgggcga caatattttt tacggtcagt cgtttacgca aacattgaaa caggcggcag
     1441 cgcaaacgca cggcgcaacc gtgtttgctt atcaggtcaa aaaccccgaa cgtttcggcg
     1501 tggttgaatt taacgaaaac ttccgcgccg tttccatcga agaaaaaccg caacggccca
     1561 aatccgattg ggcggtaacc ggcttgtact tctacgacaa ccgcgccgtc gagttcgcca
     1621 aacaaatcaa accgtccgca cgcggcgaat tggaaattac cgacctcaac caaatgtatt
     1681 tggaagacgg ttcattgtcc gtccaaatat taggacgcgg tttcgcatgg ttggacaccg
     1741 gcacccacga gagcctgcac gaagccgctt cattcgtcca aaccgtgcaa aacatccaaa
     1801 acctgcacat cgcctgcctg gaagaaatcg cttggcgcaa cggttggctg acgaaaaaag
     1861 atgtggaaac acgggcaaag cctttggaaa aaaccgccta cgggcaatac ctgctgcgcc
     1921 taatcggcaa ataaatgccg tctgaaccac tttcttcaga cggcatacag agaaaatcat
     1981 gaacatcatc gacaccgccc tccccgacgt aaaaatttta aaaccgcaag tcttcacaga
     2041 cgcgcgcggc ttttttatgg aaaccttccg cgacgagtgg tttaaaaccc aagtctgcga
     2101 acgcaccttc gtgcaggaaa accactccaa atccggcaaa ggcgtattgc gcggcctgca
     2161 ctaccaaacc gaaaacacac aaggcaaact cgtacgcgta gttgtcggcg aagtgttcga
     2221 cgtggccgtc gatatgcgcg aaggttcgcc gactttcggc aaatggacag gcgcaaccct
     2281 gtcggcacaa aaccaatacc agctttggat acccgaaggc ttcgcgcacg ggttttgcgt
     2341 tttgggcgac gcagcggaag tcgtgtacaa atgcacggac tattacaacc ccaaagccga
     2401 acactcgctg atttggaatg atccgaccgt cggcatcgac tggccattgc aaggcgagcc
     2461 gaacctgtcg cctaaagact tggcaggcaa agtattggcc gaagcagtaa cgtttggata
     2521 acttcaaaac ataacagaat caactgtttc tattttgtag tattagaaag tttcaaatga
     2581 atttaaataa aattagaaaa ttttttagag agtcccatat tttctttagg gattttctaa
     2641 ataaacgctt tcctccttat aatgtagaac agcctattcc tgaaactgaa gaattcactc
     2701 tgatcaatgc agatgccaat cttgcatcac tcacagaaaa cgttttgcca aactttccca
     2761 tcgatgttgt ttttacttgg gtcgacaata ccgataaagc atggcaagaa cagtattacc
     2821 gtaccctgca gcccatcgat caggaagata tcggcctgta tgcgaccgac cctgcccgct
     2881 tttccaacca taatgaactt ttttattcag tacaggccgt acaaaagttt atgccgtggg
     2941 tccgcaatat ttttattgta acggcggatc aaaaacctaa atggttagat gaaaacatcc
     3001 attctaaaat taaattaatc aatcattcgc aacttattga tgccaaatac ttaccaacgt
     3061 tcaattccca cgtcattgaa gcaaacttat ataaaattcc agatttaagt gaacatttta
     3121 tttatttcaa tgatgatgtt ttcgttgcaa gaccactgat gcctaaccac ttttttgaaa
     3181 ataatggttt ggcttcccta tttgttgcca ataaaagttt tcaaaaaatg cgtcaacggg
     3241 gtctaatcac gccgacattg accgcatcag aacacgcatt aaagctctta aaaagacatt
     3301 atccgacaat taatatcaat acaccgttag tacatactta cgttccgcta cggaaaacat
     3361 cctttcaaaa agcatggtca ttatttgaag atgaaataaa ttctttccta aataacaagg
     3421 ttcgccataa ttctgaactt aatatggcca gtttcttgat accctggttg atgtaccttg
     3481 acggatatgc aacaccgaaa cgtgaaattt gctactattt caatatacgc agttcacacg
     3541 cccagactca atataaaaaa ttattatttg aaaaagaaca tcttcatatg ccccactcct
     3601 tctgcattaa cgattcaagc agtaataatg cggataaaaa ctatgccctt catttccgta
     3661 attttatgga cacttacttt gaaatagaga cagaataaat ttttacatta atgatactta
     3721 atagataaca taataattta aaatgatttt ttcaacattc ggcctattta aacaacgtct
     3781 cttgctaacc aaatttttag acaaaatttc aacggataag accgcattat tttggggcaa
     3841 gaaacatccc aaacgtagaa aattagcaca tatcttaaga ttgtccttgc tcaatcttga
     3901 agacggcttt ttacgatcgc tcggactggg tgtcgccggc tatcctccct attccatcgt
     3961 ctatgacgac atcggcatct actacgacac cacccgtcct tcgcgtttgg aacaactgat
     4021 tcttgccgcc gataccatgc cgtctgaaac cttggctcag gcgcggcagg cgatggattt
     4081 catcctgcaa caccacctgt ccaaatacaa ccacgcgccc gaactttcag acgatcatcc
     4141 tttacgctcc ccatccaagc ctgaaactgt cctcattatc gaccaaacct tcggcgatat
     4201 ggccatccaa tatggcggcg cagacgcctc tacgtttgaa ctgatgtttc agacggcctt
     4261 gaatgaaaac ccgcaagccg atatctgggt aaaaacccat cccgatgttt tgtgcggcaa
     4321 aaaacaaggc tatctgaccc aactggcgca gcaacaccgc gtccatcttc tggcagaaga
     4381 catcaacccg atttctttgt tgcaaaacgt cgataaagtt tattgcgtta cctcgcaaat
     4441 gggttttgag gcacttttgt gcggcaagcc gctgaccacc ttcggcctgc cgtggtatgc
     4501 cggatggggc gtgagtgacg accgccatcc taaaatcgac agccttgttc aaacccaacg
     4561 ccgcgccccc cgcaacttgc tgcagctctt cgctgcagcc tatctgcaat acagccgcta
     4621 cctcaacccc aataccggcg aagcaggcag cctctttgat gtcatcgact atctggcgac
     4681 ggtcaaacgt aaaaacgaca aattgcgtgg cgagttatat tgcgtcggta tgtctttgtg
     4741 gaaacgcgcg gttgccaaac cgttctttaa cgtaccctct tgccgtctga aatttatctc
     4801 ttccacccaa aaactggccg gggtcaaact gtccgacgat gcacgcatcc tggcttgggg
     4861 caacggcaaa gaggccatcg tccgctttgc cgaacaacac cacatccccc tgctgcgcat
     4921 ggaagacggc tttatccgct cggtcggact cggctccaac ttagtgccgc cgctgtcgct
     4981 cgttaccgac gatatgagca tttatttcaa tgccgaagcc ccatcccgcc ttgaacacat
     5041 cctgcaaaac caaaacttcg acgatcaaga ctttcagacg gccttgaagt tgcaaaaaat
     5101 gctgaccgaa aaccatatca gcaaatacaa cgtcggcaac tcagacttta ccgccccatc
     5161 aaccgacaaa accgtgatcc tcgttcccgg tcaggttgag gatgatgcgt ctatccgcta
     5221 cggttcgccc caaatctatc acaatttgga tttgctccgt accgtacgcg aacgccatcc
     5281 cgatgcctat atcatctaca aaccgcatcc cgatgtagtc agcggtaacc gcatcggcca
     5341 tatttcccct gaagatgctg cacgatatgc cgaccaaacc gccgaacaag ccgacatcct
     5401 gacctgtctc caatacgcag acgaaataca taccatgact tcgctgaccg gttttgaagc
     5461 cttgttgcgc ggcaaaaaag tcagctgcta cggcctgcct ttttacgcag gctgggggct
     5521 tacccaagat ctgctcccca tcccgcgccg tagccgcaga cttgagcttt ggcagctgat
     5581 tgccggcacg ctcatccact atcccgacta catccacccc gaaacccatc aggccataaa
     5641 tgcagaaacc gcagcccaaa tcctgatacg acaaaaaaat atgcaaaaaa acaacaacgg
     5701 attacatcgc gggtgctttg ccaaaaaatt aggtaaaatc aaacaactat atcgatcttt
     5761 caaataaata ccatcaaagt taacgatgcg tcataaactt gcctctattg cggcatcatt
     5821 gcctttgcat cgttaattct cttggcgtat gcttgaaagt tcaacctaaa actattacat
     5881 aaaaaacaaa accacattgc aacatgaaac agaccgtcct caaaaataac ctgcaaaacc
     5941 tgcttgaaag cgcagaaaat atcctgctgc ttcaaggccc tgtcggcgat ttttttctgc
     6001 gccttgccga ctggctgact gcaaacggca aaaccgtaca taaattcaac tttaatgcag
     6061 gcgacgacta tttttatccg cccactcaag cgcataccgt tgtttttaac gacaactacg
     6121 atgcctttcc tgagtttttg caagaataca tcactcaaca tcacatccag gccgttgtct
     6181 gctttggcga cacacgccct tatcacgtca ttgcaaaacg cattgcaaac gaaaaccaag
     6241 ccagtttctg ggcgtttgaa gaaggctatt tccgccccta ctacatcacc ttagaaaaag
     6301 acggcgtcaa cgcattttcc ccgttgccgc gccgtgccga cttttttctt gaacaattcc
     6361 ctaagcttgc ccagcaagaa tataaagcgc caacgccggt acacggcggt tttacgccca
     6421 tggcaaaaaa cgctatccgt tactatatcg agttgttccg caatccacgc aaataccccg
     6481 actacatcca ccaccgcgca cccaatgccg gccattacct caaaccgtgg tcgctctcca
     6541 tcctcaagcg tttgaactac tatattgaag acatccaaat cgccaaacgt gtggaagcag
     6601 gcaaatacgg caagtttttt attgttccct tacaggtatt caacgacagc caagtccgta
     6661 tccattgcga ctttcccagc gtccgcagct tcctgctcca tgttttgagt tcatttgccg
     6721 agcacgcgcc tgccgatacc aacatcatca tcaagcatca tcctatggac cgcggcttta
     6781 tcgactactg gcgcgacatt aaacgcttta tcaaagaaca ccccgaactc aaaggccgtg
     6841 tgatttatgt ccatgatgtc cccctgcccg ttttcctgcg ccacggtctc ggcatggtta
     6901 ccatcaacag caccagcggc ctgtccggac tgattcacaa tatgccagtt aaggttctcg
     6961 gccgtgccta ttatgatatt cccggcatta ctgaccaaaa taccttggca gaattttgga
     7021 atcatccgac accgcctgac aaagagctgt tccatgccta ccgaatgtac cacctcaacg
     7081 tgacccaaat taacggcaac ttctacagtc aggtcttttt tcctaacaaa aacacatccg
     7141 actcttccac accaacaacc taacttggcg aaagaaaagc tcaggccgtc tgaaaacatt
     7201 tcagacggcc tgaaacaatc aataccttag ctactgccat gtaaataaaa cacaaaaatc
     7261 tgcatttatc attaacaata aattacaaaa acagtataat gaccgagctg ccatgagcgc
     7321 ataccgactc aacctgagcc ctttgtaaca cacaaaatat ggatatatcc ctaggcaaaa
     7381 caatataaca agccaaacat cctaaagata agccggcaag gcaatacact ctataaaact
     7441 gtgccgagca aaatttttac aaagccctca accggtatcg ccgcccatat gccgcagcat
     7501 ccgtcttcca ctttatatcc gcccgcaaac catgaccgcc gctcctgata tcctctaccg
     7561 gcaagccgcc gcccttttgg aacaatccaa taccgcccaa gccctgcccc tgttgcaaca
     7621 ggcggcagag caaggttatg cggaagctgc tttcgtattg ggcaaccatc tgctgcaaaa
     7681 cggccaaccg gagcaggcac tttcatggtt ggaagccgcc gcggcccaac gccatcccaa
     7741 agcactcttc tccctgctgc aacaacgcga acacaacggc accccgaccg gacagcttct
     7801 caacgactat gcctggctgg gtgagcaggg gcactcagaa gcccaattaa tcctcatgcg
     7861 ttaccacgcg caacgcaacg atccacaatc gctctactgg gcggaacttg ctgccgcccg
     7921 atatgccgca cctgcgtatt accatctggc acgccatcat caacgccaag gcgacgttga
     7981 aacagccatc gaacaatacg aaaaagcggc agcactcggc gtaactgccg cctgctggca
     8041 acttggtcaa atctacttct acggtacagg tgtcagcccc aaccacgcac aagccgaaca
     8101 ctatctcgaa ccagccgcac aagccggcca catcgccgca caaacgctgc tggctgacct
     8161 tcttgccgcc caacgcaaac ctgaagcctt ggaatggtat cgtcgtgccg ccgataagga
     8221 acaagcggaa gcacaggcca agctggccca atacgccctg accggcgaac tttccgaacg
     8281 cgatccgttc caagcggcac gatatgccaa agccgctgcc gagaaaaacc atcctgaagc
     8341 cctgaaaatc atgggcgacc tctaccgcta cggtctcggt atcaaagccg acaaccatat
     8401 cgcgcaagat tactaccacc gtgccgccgc gctgggttct gccgccgcag cacaaaaact
     8461 catcagcgac gccgcgctgt accatccgca acaatacgaa caaatcaaaa ctgccgccct
     8521 gcaacaacaa caaactgaaa ccatctaccg tttggcggaa gcacaagcct gcgccatcgg
     8581 ccgtcccgcc gactacaatg ccgcgcgaaa aaattacatg gaagctgccg ggttccacca
     8641 taaaaacgca gcggcagcct taggccgcat ctaccattac ggcctcggta cggcgcaaga
     8701 tcctcgggcg gctgcacact ggtacgccat tgctgccgaa caaaaccacc cttccgccca
     8761 ataccacctc gcctgttttt actatcacgg gcaaggtgtc ggctgtcatg ttccgaccgc
     8821 ctgctactgg ctgcaggccg ccatcggcaa cggccacact tcggccgaat cattaatatc
     8881 cctattagaa caatggcgac gcgaagcaca ccatgccatc ggacaaaagg ccgtctgaaa
     8941 agatttacac tcgcattttt tgacaatctt taactattcc cctaatattt gccagttatt
     9001 tttcacggac acgccattgt tttcatttct ttctgaaaac accttgtccg cgcatcaata
     9061 ccatgacact cggcggataa cgccaagcgt tgaaacacac tacatccgga acaaaaacgg
     9121 atgctcggaa aaatatttct aggaggtgaa acaacatgga atgggaattc aacagttatt
     9181 acacactgat tgccgccacg ctcgtgttgc tggttggtaa atttttggtt caaaaaatca
     9241 aattcttacg agacttcaat attcccgagc cggtagccgg cggtttgatt gccgctatcg
     9301 tcctgttcgc cctgcacgag gcgtacggcg tgagcttcaa atttgagaaa ccgctgcaaa
     9361 atgcgtttat gctgattttt ttcacgtcca tcggcttgag cgcggatttt tcccgtttga
     9421 aggcgggcgg tttgccgctg gtggttttta ccgcgattgt gggcggattt atcttggtgc
     9481 aaaactttgt cggggtcgga ctggctacgg ctttgggttt ggatccgctc atcggtctga
     9541 ttaccggttc ggtgtcgctg acgggcggac acggtacgtc aggtgcgtgg ggacctaatt
     9601 ttgaaacgca atacggcttg gtcggcgcaa ccggtttggg tattgcatcg gctactttcg
     9661 ggctggtgtt cggcggcctg atcggtgggc cggttgcgcg ccgcctgatc aacaaaatgg
     9721 gccgcaaacc ggttgaaaac aaaaaacagg atcaggacga caacgcggac gacgtgttcg
     9781 agcaggcaaa acgcacccgc ctgattacgg cggaatctgc cgttgaaacg cttgccatgt
     9841 ttgccgcgtg tttggcgttt gccgagatta tggacggctt cgacaaagaa tacctgttcg
     9901 acctgcccaa attcgtgtgg tgtctgtttg gcggcgtggt catccgcaac atcctcactg
     9961 ccgcattcaa ggtcaatatg ttcgaccgcg ccatcgatgt gttcggcaat gcttcgcttt
    10021 cgcttttctt ggcaatggcg ttgctgaatt tgaaactgtg ggagctgacc ggtttggcgg
    10081 ggcctgtaac cgtgattctt gccgtacaaa ccgtggtgat ggttttgtac gcgacttttg
    10141 ttacctatgt ctttatgggg cgcgattatg atgcggcagt attggctgcc ggccattgcg
    10201 gtttcggctt gggtgcaacg ccgacggcgg tggcaaatat gcagtccgtc acgcatactt
    10261 tcggcgcgtc gcataaggcg tttttgattg tgcctatggt cggcgcgttc ttcgtcgatt
    10321 tgattaatgc cgcgattctc accggttttg tgaatttctt taaaggctga ttttccgcct
    10381 ttccgccaaa gcacctgcaa ggtttaccgc ctgcaggtgc ttttgctatg atagccgcta
    10441 tcggtctgca ccgtttggaa ggaacatcat gtatcggaaa ctcattgcgc tgccgtttgc
    10501 cctgctgctt gccgcttgcg gcagggaaga accgcccaag gcattggaat gcgccaaccc
    10561 cgccgtgttg caaggcatac gcggcaatat tcaggaaacg ctcacgcagg aagcgcgttc
    10621 tttcgcgcgc gaagacggca ggcagtttgt cgatgccgac aaaattatcg ccgccgccta
    10681 cggtttggcg ttttctttgg aacacgcttc ggaaacgcag gaaggcgggc gcacgttctg
    10741 tatcgccgat ttgaacatta ccgtgccgtc tgaaacgctt gccgatgcca aggcaaacag
    10801 ccccctgctg tacggggaaa ccgctttgtc ggatattgtg cggcagaaga cgggcggcaa
    10861 tgtcgagttt aaagacggcg tattgacggc agccgtccgc ttcctgcccg tcaaagacgg
    10921 tcagacggca tttgtcgaca acacggtcgg tatggcggcg caaacgctgt ctgccgcgct
    10981 gctgccttac ggcgtgaaga gcatcgtgat gatagacggc aaggcggtga aaaaagaaga
    11041 cgcggtcagg attttgagcg gaaaagcccg tgaagaagaa ccgtccaaac ccacgcccga
    11101 agacattttg gaacacaatg ccgccggcgg cgatgcgggc gtaccccaag ccgcagaagg
    11161 cgcgcccgaa ccggaaatcc tgcatcctga cgacggcgag cgtgccgata ccgttaccgt
    11221 atcacggggc gaagtggaag aggcgcgcgt acaaaaccag cgtgcggaat ccgaaattac
    11281 caaactttgg ggaggactcg ataccgacgt gcaaaaagag ttggtcggcg aacaacgcaa
    11341 gtgggcgcag gaaaaaatca gcaactgccg acaagccgcc gcgcaggcag accggcagga
    11401 atacgccgaa tacctcaagc tgcaatgcga cacgcggatg acgcgcgaac ggatacagta
    11461 tcttcgcggc tattccatcg attaggggca aaccgatgac taccgtcccc aaaagcagga
    11521 ttcccgtcaa accgctgccc gaaaaaacca cagacgaagc caaagtcgaa aaatggcggc
    11581 agctcggtgc ggaacacggt ttgtcgggcg aatgggcagt tgccgccaga ttgggcgaga
    11641 acggttttac cgaagaacag atggaaaata tcgccaacct gttcggcaga taaagggaaa
    11701 attgacggaa atgccgtctg aaaccctgtt atcggtttca gacggcattt tgactaatac
    11761 ggtacgcagg cgcaaaacag ccggcttttc ctgtgttgcc tatgctgatg tttcaacaca
    11821 caggacgata caaaaaacgt cgccctatgt gccgtcctga ttcggaaagg gttacgctcc
    11881 tcccaa
//
LOCUS       DAWHRS010000051        11817 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWHRS010000051 DAWHRS010000000
VERSION     DAWHRS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11817
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.051"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(67..3165)
                     /locus_tag="WIB82_000989"
     CDS             complement(67..3165)
                     /locus_tag="WIB82_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ3842303.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANNTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTG
                     EVRRGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEALNETA
                     AKRYLISSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(3196..3308)
                     /locus_tag="WIB82_000990"
                     /pseudo
     CDS             complement(3196..3308)
                     /locus_tag="WIB82_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3287..3472)
                     /locus_tag="WIB82_000991"
     CDS             complement(3287..3472)
                     /locus_tag="WIB82_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842304.1"
                     /translation="MGFSPPKQQQAATTVKQRQAGRWAEAHPTMDTTDPHSAAANQSS
                     LHIFRQPNPNIKPFIVN"
     gene            complement(3474..4019)
                     /locus_tag="WIB82_000992"
     CDS             complement(3474..4019)
                     /locus_tag="WIB82_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ3842305.1"
                     /translation="MGEIAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSGYVPSE
                     GKMTEYIANDILIGNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQVLADD
                     KFFAFNMKHAKGAKMPRGSKAAIMQYKIPIPPLPEQEKIVAILDKFDTLTHSVSEGLP
                     YEIALRRKQYEYYREQLLAFR"
     gene            complement(4060..4664)
                     /locus_tag="WIB82_000993"
                     /pseudo
     CDS             complement(4060..4664)
                     /locus_tag="WIB82_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(4786..4878)
                     /locus_tag="WIB82_000994"
     CDS             complement(4786..4878)
                     /locus_tag="WIB82_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible D domain protein"
                     /protein_id="HEZ3842306.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSVCTLQTK"
     gene            complement(4995..6539)
                     /locus_tag="WIB82_000995"
     CDS             complement(4995..6539)
                     /locus_tag="WIB82_000995"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ3842307.1"
                     /translation="MMTEIQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDGRPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDASGFFKKETNNNVLTEEHIADIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEIGETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(6612..7616)
                     /gene="rfaD"
                     /locus_tag="WIB82_000996"
     CDS             complement(6612..7616)
                     /gene="rfaD"
                     /locus_tag="WIB82_000996"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ3842308.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VKEELIRYIPFPNALKGKYQSFTQADITKLREAGYKEEFFDVKSGIDRYVKWMLENLA
                     "
     gene            complement(7657..8775)
                     /locus_tag="WIB82_000997"
     CDS             complement(7657..8775)
                     /locus_tag="WIB82_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ3842309.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFYKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDISDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KSYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(8777..10012)
                     /gene="dcm"
                     /locus_tag="WIB82_000998"
     CDS             complement(8777..10012)
                     /gene="dcm"
                     /locus_tag="WIB82_000998"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3842310.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGAKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(10049..11020)
                     /gene="hldA"
                     /locus_tag="WIB82_000999"
     CDS             complement(10049..11020)
                     /gene="hldA"
                     /locus_tag="WIB82_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ3842311.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(11075..11815)
                     /gene="pyrF"
                     /locus_tag="WIB82_001000"
     CDS             complement(11075..11815)
                     /gene="pyrF"
                     /locus_tag="WIB82_001000"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ3842312.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
BASE COUNT         2753 a         2762 c         2762 g         3540 t
ORIGIN      
        1 tccactataa aaaccgcctc tgcataaaat caaaaatgcc gtctgaaagg ttcagacggc
       61 attttgtcaa atatcggtcc ctattccggc aaacttctcc acaaacgccg caatcttttg
      121 aaaaacactt tgtttcttcg tcagatattg cggattgagc ggactcattt tcggcagggt
      181 ttcggtcagt tccgtgccgt tttcgctggc atagccgcgt ttgagcgagc tgattaaata
      241 gcgtttagcg gcggtttcgt tcagggcttc ggcggcaatc aatcctgccg cttcgtgccg
      301 catcacctct tgcgcgtagg tgtaaaaggt ttccagtatg gcgggaacgt cgggcacttt
      361 gtccaaatcc gtatcgttga tgaaatccac aatcagaccc tctttggcgc ggtgtccgat
      421 acttgcgcgg atgatgcggc ggatttcttc caccagctcc gctttgcctt tgattttttt
      481 gtggtgttcg aaaaccagtt gcaaaatgta atccaaattg atttcctgcg atttgagcaa
      541 atccacctca aaaaccacat cgtcccaatc gattttcgat tgctctttct gttcgcctgc
      601 tttttggcgg cgcaaccaat cgcggatgtc gttgtaggcg gaacggtagt cctgcaccgc
      661 cctttcagac ggcatcggca cttgccgcat ttcctgcacg tcttcatcgc tcaagtagta
      721 tttttcttga aacgccttca tcgcatcttc gtccgccgcg tccacactct gcaactcgcg
      781 cagcgcggca aattcatcgt agttctgcaa tacgttttcc gcccgcaggt attcgccgaa
      841 gagtttggca aaatcttttt tgtctttttc cgtttcgatt ttgtcgggat cggggaaacg
      901 ctcgcgcaat tcttttgcca catccaaata accgcgccgc acttcgcccg tccgatcatc
      961 gatatagccg ttcatgtatt cttcgtagct tttttccaac accacgtttt tggtgttttt
     1021 atcgccgaac agggtaatcg catcaatggt tgcctgctcc aaatcgcgga agcagacaat
     1081 attgccgaag gttttggtgg catcgtaaat gcggttggtg cgcgaaaacg cctgcatcag
     1141 gccgtgatag cgcaggtttt tatcgacaaa cagcgtgttc agcgtcggcg cgtcaaaacc
     1201 cgtcaaaaac ataccgacca caatcagcaa atccacttcc tggtttttca cccgttttgc
     1261 caaatcgcgg tagtagttct gaaaggcttt gctgtccgtg ccgaaattgg ttttgaaaca
     1321 ggcgttgtaa tcgttgatgg cagcctgcaa aaattctttc gcgctgctgt ccatcgcttc
     1381 cggttcaaag gtctcatcaa caatttcacc gacggcgttc tgctcttcgt tggccgcaaa
     1441 ggaaaaaatg gtggccactt tcagcgggtg caggctgcct gcctgttgtg ttttgaacgc
     1501 ttcgtaatag cacttcgccg catccacgct gctgaccgca aacatggcgt tgaagccttt
     1561 gccgcccgca ttcaggcggt gcgttttctg cctgaactgg ttcaggatat attgcgtgat
     1621 ttcgcggatg cgttcggggt gcagcagggc ttggcggttt tcggcagcac tcagtttctt
     1681 ctcatcctgt tccgcttcca cggctttgaa ctgcgggcgc acatcgttgt aatccacttt
     1741 gaatttcaat actttttcat cgcggatggc atcggtaatc acataagaat gcaactcacg
     1801 cccgaacacg cccgccgtgg tttccgcgcc caaagcgttt tcgggaaaaa tcggcgtacc
     1861 ggtaaagccg aactggcaga attttttaaa tttctttttc agattttttt gcgcttcgcc
     1921 gaattgcgag cggtggcatt cgtcgaaaat aaagacaacc tgctggtgat aaaccggcag
     1981 attatcttca ctcttcatca ggttgttcag cttttggatg gtggtaacga tgattttgtt
     2041 gtcgtctttt tccaaattgc gtttcaaacc cgccgtgctt tccgaaccgt ttacgctgtc
     2101 gggcgaaaaa cgctggtatt ccttcatcgt ctggtagtcc aaatccttcc tgtccaccac
     2161 gaagaaaacc ttgtcgataa acgccgattc cgtcgccaga cgcgccgcct taaagctggt
     2221 cagcgttttg ccgctgcccg tggtgtgcca aacatagccg ccgctttccg gcccgctcca
     2281 attcttcgcc tgcgccgagc tgttgatttt ccacaaaata cgttcggcgg cggcaatctg
     2341 gtacggccgc ataatcagca gcgtattatt cgcatcgaac acgctgtaat gcagcaaaac
     2401 gcccagcaat acgcttttct gcaggaacgt ggcagtaaag tctttcaaat ccttaatcgg
     2461 atgattgtcc gaccgcgccc aattcatcgt gaaatcgaag ctatttttgt cgcgcttggt
     2521 ggtgttggcg aaatagcgcg tgtccgtgcc gttggaaatc acgaagattt gcaggaattt
     2581 gaacagcgaa ttttcgctgt tgaagctctc tttgctgtaa cggtgcacct gattgaatgc
     2641 ttcgcgcacc gccacaccgc gctttttcaa ttcaatctgc accagcggca gaccgtttac
     2701 caacacggta acgtcataac ggtttgcatg cgtgcccgtc tgctcaaact ggttgataag
     2761 ctgcacatgg ttgcgggcaa ggtttttctt gtccagcaga taaatgtttt tcagacggcc
     2821 gtcatcaaaa gcgaaatcgt aaatatggtc gtcgtggatt ttgcgggttt tatcggtaat
     2881 attttcagac ggtctgtcca gatattccgt caaaaaccgc gcccattcgc cgtctgaaaa
     2941 cgccacatcg ttcagccgct gcaactgcgc gcgcaggttt tccagcagcc tgctttggct
     3001 gttcaaatcc ttgcggtatt cgtacccctg attctgcaaa tcggcgatta actccgcttc
     3061 caaccggttt tccgattggt agctgcccga ctgttcgatt ttttcatatt ggtcgagcac
     3121 gatgaaattc ggcgtttcag cgatgggttt agtttcgagg ttcatatggg tcgtcctttg
     3181 cggtgtcggg ttttatagtg aattaaattt aaaccggtac tgcgttggct cgccttgccg
     3241 tactatttgt actgtctgcg gctcgccgcc ttgtcctgat ttaaatttaa ttcactataa
     3301 acggtttgat gttcggattg ggctgcctga aaatgtgcag gctgctttgg ttggctgcgg
     3361 cggaatgcgg gtcggttgtg tccatcgtag gatgggcttc agcccaccgc cccgcctgcc
     3421 gttgttttac tgttgttgca gcttgctgct gtttcggtgg gctgaagccc accctaccga
     3481 aaggcaagca gctgttcgcg gtaatattcg tattgtttgc ggcgcagggc gatttcgtac
     3541 ggcaggcctt cgctgacgga gtgggtcagg gtgtcgaatt tgtctaggat ggcgacgatt
     3601 ttttcctgtt cggggagtgg tgggatgggg attttatatt gcataatcgc tgctttgctg
     3661 ccacgcggca tttttgcacc tttggcgtgc ttcatattaa aggcaaaaaa tttatcatcg
     3721 gccaatacct gatacagata ttttggatta accttttcat ctgttacccg gataaccaaa
     3781 acatcaccgt ttgttccgcc cgtgcagtct gcctgccaga tttttttcaa atacggacga
     3841 atatttccaa tcaagatgtc attggcaata tattctgtca tttttccctc acttggaaca
     3901 taaccggata actttttacc ttctctattt tgtaagagat tatccacgcc cacgtaatta
     3961 tgttcgttca gtttatccga acaaatacgg ttttttgaat attcggcaat ctcccccaac
     4021 gtcttccaaa ccacatcttt cagacggcat tgatagccat cagctatccc ccccgatttg
     4081 attgtcaaaa tctaaaagaa agtcgcggta ataccggtat tggcgtttgc gcagggctaa
     4141 ttccgcttcc agcgtagctt ccagcgtagc ttccagctcg gtgaatttgt caagtatttt
     4201 tacaattttt tgttgggttt ctaggggcgg gatgggaata agtaagtttt gcaattcact
     4261 tttagcaagt gctggtatac ctgctccgta ttgacattgc atgaatgtaa tttgcttcat
     4321 ttttaaaaat aatatgcaaa ccgttgcaat agtttgtctt cgttcgcttt aatagtatat
     4381 gcgtgagctc ctgcaaaaaa agggatatcc atccaattaa caaaccccgc acttgctcct
     4441 ccttggctaa taacaatcgt gccagcttcg ttattgtatt catgccaata acctgatgga
     4501 ttaataccgc cattgattac gggatactta tctttttctc taagtagtga tttattaagc
     4561 tgctttccct ttcctatact tgccacttcc cccaacggct tccactccac cggtgccgtc
     4621 tgaatcatct caatcaattt tttcgcttta ctttgtatat ccatggttgt tttccgtttc
     4681 tttcccgctg cggcgggatg tggggcggtt atccgccgta gggtgggctc cagcccaaca
     4741 atccgcctgc tgttttgccg ttgtttcggg gggctgaagc ccaccctact ttgtctgcaa
     4801 ggtgcacacc gaccaatatt cattgcctgt ttcgtcgatg tgtttggcgt tttcaaaagc
     4861 gttgttttct gttgtcatgt atttttcccc gaaaatttct tgatttgaac ttgtaaagat
     4921 tacttacagg ttaccgcccg tattttcaga cggcctcttt cccattcagt tgttgcaaaa
     4981 tatgcaacaa ctgcctaggc ctcaatctct gcaatcactt cgtcaatttc gcgccgcagc
     5041 cgttcgattt tggcgacggt ttcgccgatt tcggcgttaa gctgtttgat gtcgataatt
     5101 tcgcgtgtgt cttcgggttc aacatagctg ctgacggcga ggttgtagcc gttgtctttg
     5161 acggtttgct gggcggcgtt ttgggcgata tgcggcacat cggctttgtc ggcgaagagt
     5221 ttgacgatgt cggcaatgtg ttcttcggtt aagacgttgt tgttggtttc ttttttaaag
     5281 aagccgcttg cgtcgataaa ttggatgtcg gtattgtctt tgtgtttgga caaaaccaag
     5341 atattgacgg cgatgccggt gccgtaaaag agattgggcg caagggcgat gacggtttcc
     5401 acgtagttgc cctccaccag atattggcgg attttctgct ctgcacctcc gcgatagaaa
     5461 atgccgggga atgagacgat ggcggcgcgg cctctgccgg aaaggtagtt cagtgcgtgc
     5521 aggataaagg caaaatcggc tttggatttc ggggcgagta cacctgcggg ggcaaagcgg
     5581 tcgtcgttga ttaaggtggg gtcgtcgctg cctatccagt tgatggaata aggcggattg
     5641 gaaacgatgg catcaaaggg cctgccgtct ttgagcttgg gattggtcag tgtgtcgccc
     5701 aattcgatgt ggaatttgtt gtaattgacg ttgtgcagaa acatgttcat gcgagcgagg
     5761 ttgtaggtgg tgtggttgat ttcctgcccg aagaagcctt cttcgatgat gtgctcatca
     5821 aactgttttt tcgcctgcaa aagcagactg cccgagccgc aggcgggatc gtaaatttta
     5881 tttacctttt cctgcccgtg caccgccagc cgcgcaatca gcttggagac gctttgcggg
     5941 gtaaaaaatt cgccgccgga tttgcctgcg ttggcggcgt agttggaaat caggtattcg
     6001 taggcatcgc cgaaaaggtc gatgcggtgg tcttcaaaat tgccgaaatc gagttccgcc
     6061 acgcctttga ggacggcggc aaggcgtttg tttttgtcgg caacggtgct gccgagccgg
     6121 ctgctggtgg tgtcgaagtc gtcaaacagg cctttgatgt cttgttcgga cggatagccg
     6181 gaggcggagc tttcaatcgc ggtaaaaatt tctttcagct tggtgttgag ctcttcgttt
     6241 tgatgggctt cggcggcaat attgcaaaaa agctggccgg ggtagatgaa ataacctttg
     6301 actttgacgg catcgtcttt gatttcgggt gtgatgatgc tgtccggcat agcggcgtaa
     6361 tcaatactgc tgtcgcctgc ctgcatatag tcggtgaagt tttcgctgat aaatcggtag
     6421 aaaagtgtgc cgagaacgta ttgtttgaag tcccagccat ccaccgcgcc gcgtacttcg
     6481 tcggcaattt tccaaatttg gcggtgcagt tgggcgcgtt gttgtatttc ggtcatcatc
     6541 gaaatccata taaaagttaa acaaatcaaa atcgcctgat attttcagac gattttttac
     6601 gggcattcaa attaagccaa attttccagc atccatttga cgtagcggtc gatgcctgat
     6661 ttgacatcga aaaattcttc cttatatccg gcttcgcgca atttggtaat gtcggcttgg
     6721 gtgaagcttt ggtatttgcc tttgagcgca ttgggaaatg ggatgtagcg gataagttct
     6781 tcttttacca gctctttcaa gctcatttca ggtttgcctt cggcggcgcg gcaggcgttg
     6841 acggtggcgg cggcgagttc gttaaactgt tggctgcggc cggtaccgag gttgtagatg
     6901 ccggaaagtt cgggatggtc gaagaagtag aggttgactt tggcaacgtc ttcgacgctg
     6961 acaaagtcgc gggtttgttc gccgttgccg tagccatcgt tgctgccgaa caggttgacg
     7021 taaccgtgtt cgcggtattg gtggaaatgg tggaaggcga cggatgccat gcggcctttg
     7081 tgttgttcgt gttgtccgta aacgttgaag tagcggaagc cgacgacttg ggcggtcaag
     7141 ccttctttca tacggcgacg caatacttgg tcaaacagga atttggagta gccgtacacg
     7201 ttgagcggtt tttcgagttc gcgctcttcg cggaagattt cgcctttgcc gtaaaccgcc
     7261 gcactggagg cataaaggaa ggggatgcgt tcgtcctgac accagtccag caaatccagc
     7321 gtgtactggt agttgttgtc catcatatac aaaccgtcgt ggttcatcgt atcggaacac
     7381 gcgccttgat ggaaaacggc ttcgatgttt tgataaggta aaatgtgttc cctcacttgg
     7441 cggatgaatt catgtttatc gagataatgg gcgatttcgc actcggcaag gtttttgaat
     7501 ttttcgcctt tgctcaaatt atcgacggca acaatgtcag taataccgcg ttgattaagt
     7561 gctttgacga tgttgctgcc gataaagccg gccgcgcctg ttacgatgat ggtcatattc
     7621 ggtttccttt gttttgagta aaattaataa acttagttac aagaaatcat cgatattgtc
     7681 ctgaaacaca aatttgcgat gatattccaa atatttttct cttccttcgg gatatattct
     7741 catcttattt tttgcatttg gattgatatt aagtttttcc catgtgtaac ggcttagtcg
     7801 agtaccgcaa atcaaacgac catcatccaa gaaagtaata taaccttgat cgaacaacca
     7861 atcataagtc ggcgttaaag ctaacccatt taaataatct aatgcctctt tctcattttt
     7921 ctcagtaata caaaccatat aagatttaat atgactagcc cttaacaaaa tctcatctgt
     7981 aattaatgta aatggacatt gcggcatatg atttataaca tccttgcgat atttctgctg
     8041 cccttttcta cttttctgtt taatgatttc ttgctctacg aattgattaa attctgaagc
     8101 cggtatttct aatttttctc ttgataagag ttgcgggtgc acgatagagc gaaattgata
     8161 atccaaaaat attcggaaat aaaataatgg ttctgaatct tctatatctt tgacaggcaa
     8221 tagtttaaga attgataaat aactaatttt gggcaataca atcttacgcc aaattgacca
     8281 tatatcatcc tccgaacgga tataacccct acggtaacct ttagaatttt ccaaatggtc
     8341 tgatatatca tggattgaaa agaaaatatt ttctgtaggc agattattaa tacttaaaac
     8401 gtaatcatca taaaaccttg aaatatcatg atgatatttc tgttcttgtt tgtgatattc
     8461 aacttttgca tcaaccaaat aacgctgtaa atttttctta taaaaaaaac aatttttctg
     8521 aatgaaagga tattctaccc tccttccatt ttttgtctca gtaatccccc aattttcata
     8581 ctcaaaaaat gtacttagtt ttcgaccact atcatcaata taactaccta catatttgcg
     8641 tgcttcccca ttaccaccaa aaacagataa cttgttattc ctgtgaataa aactatcttc
     8701 cgcacgtaaa ttttgaatac tatcgacaat aaaatactct atgttttcaa tagtaattct
     8761 ttgaaaatcc ggcattttac tctccataaa gcgtaatatc catttgtttt aatatagcga
     8821 ttaatacatc aacaacaata ctgtttcccg cttggcgata catttgagta tcactaacta
     8881 aaattttaaa atcatctcta aaacccatta gccgcaaaca ttccctaggt gttaatttac
     8941 gaatacgtcc acgattatga gtaacataat tatcaacccc ggcacgatgc attttatgca
     9001 tagtttgcaa taatgggcga gctacaggca aatcagtttc cgtactggtt ttaaaatttt
     9061 ttgttcctcc tgccaaaaca taatttttga ttttttcaga aagataatat ttttcctcaa
     9121 cgttatttac atcaaaaata aagtcatcaa attcagattc gcgggctgcc tgaaaaataa
     9181 aatcaccatg ccaattaaat tgttgatttg ctttttggca taaggcaatt tctccattaa
     9241 tctgtgtata acgtttttgt ctatttttag aactggtaac aaatttcgca cccttttcac
     9301 gtaaaaaata tttgctatca gtatagtcct ccaaaaaatc ttgcatagta tgttctaatt
     9361 caatcttttc aggaaactga aaaccattta taggaggggt atgaaagcca acaacaaaaa
     9421 tacgctcacg atgttgagga atcccataat ccttactatt cattatttgg aaatataagt
     9481 cataaccaag tgaataaaaa acacttttta caactttcca agtttttcca ttatcatgat
     9541 taagcaagcc ctttacattt tcataaataa atatctttgg ttggacttca tccacaacgc
     9601 gagcaaattc atagaataat gttcctcgtg tatcttctaa tcctgcacgt ttgccaacca
     9661 tggaaaatgc ttggcaagga ctgcctccaa ctaaaatatc aacttgattt ctaaactttc
     9721 tcgcatcaaa ttgagtaatg tcgttatgcc aaaaatcttc atttaattta tagtttccaa
     9781 gataactttt tttaacgtat ggatcaatat ctcctgaaaa aacaatggta tggtttaaat
     9841 ttaatcggtg gaatgcctgt tcaaccgcac caatcccgct gaatacggtt gctaatctaa
     9901 tatgtgaatc aggtttaaga aaagttttag atttccaacc ttgttgactg ggaaagagca
     9961 aagttttttg taatcgagta tcgtgtgtct gtgccattgt cgaaatagtc atacttatat
    10021 cgttctgttt atcttatcaa tatgaaaact acattgttga ttgccctgac aatgccttga
    10081 tcaattcggc aaacgagcaa accgccgtac cgagtttcgc cacgacaacc ccggccgcag
    10141 tattggcaag gtacatggct tcgggcatgg tgcagcctgc cgccaaaccc aagcccattc
    10201 cggcaatgac ggtgtcgccc gcaccggata cgtcgtaaac ttcttgggcg cgggtgggct
    10261 ggtaaatcgg ttcgccttcg ctgaacaagg tcatgccttc ttcgcttcgg gtcagtaaaa
    10321 cggcggtcag gtcgaggtgg cggcgcaggt tttgcgcttt ttcggtcagc tcgctttcgt
    10381 ttttccaact gccgaccact tctttcaatt cggcgcggtt aggcgtaatc agagttgcac
    10441 cgacatattt ttcgtaatcg tcgcctttgg ggtcgattaa gacggttttg ccggcgtgtt
    10501 tcgcccaatc gatcatatcg gagatatgcg acaggccgcc tttgccgtag tctgaaaaaa
    10561 tgattgcgtc gtattcgggc aagatttcgc ggtatttctg cttgatttgt tccaacactt
    10621 cgcagttggg atgttcttca aaatcaagac ggataagctg ctggttgcgg gcgacgacgc
    10681 gcagtttgac ggtggtggcg atttgtttgt cgcgcatcag ataggaggcg acgccgtcct
    10741 gcaccatcag cgcatcgagc gcgtcggcgg cttcgtcgtt gccggttacg gacaacagcc
    10801 ctgccctgcc gcccaacgaa gcgatgttgc gcgcgacatt tgccgctccg cccgcgcgtt
    10861 ggtcgattcg tccgattttc gccaccggca cgggggcttc gggcgaaata cgggacacat
    10921 cgccgaacca atagcggtcg agcatcacgt cgccgacaac caggactttg gcttgcgcga
    10981 aacgggattt gagggtttct tgttggaact tggcggacat attgcctccg ttgtattttt
    11041 tcagacggcc tcagcctgta aggccgtctg aaaatcagtt tgcttcaagg tttgccacgc
    11101 ggttcacttc gcgcaatacg gctaccggat cggcagcttg ggttacagga cgacccatta
    11161 ccaaataagt cgaaccggca gccaaggctt cggccggtgt catgatgcgg cgctggtcat
    11221 cattattgcc ggcaacgtcc aagcggatgc cgggcgtgac caagacaaaa tcctgtccca
    11281 attcgcggcg cagcggcgcg gcttcttggg cggaacagac cacgccgtcc aagcccgaac
    11341 tttgcgccag ttttgccaag cggatgactt gttcttcagg ggcggtgttc aaaccgattt
    11401 ccgccaaatc actttgttcc atgctggtca acacggttac gccgattaag agcggcttcg
    11461 tgccgtatcc ggcaacggct tctgctgcgg cttccatcat acggcggccg cccgatgcgt
    11521 gcatatcgac catccaaacg cccatatcgg cagcgacttt gcaggcttgc gcgacggtgt
    11581 ggggaatatc gtggtatttc aaatcgagga aaagtttgaa accttgattg attaagcttt
    11641 ctgccaaatt gcgtcccgtc gcggtaaaca gctctttgcc gattttgatt tgacacaatg
    11701 tcgggtcaag gttgcggacg aatccgagcg tgtctttttc gttggaaaaa tcaagggcga
    11761 cgataacggg ggtgcgttgt tgcggagttt ggaagtcgga gattaaagga ttcatgg
//
LOCUS       DAWHRS010000052        11798 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWHRS010000052 DAWHRS010000000
VERSION     DAWHRS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.052"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            258..1253
                     /gene="fbpA"
                     /locus_tag="WIB82_001001"
     CDS             258..1253
                     /gene="fbpA"
                     /locus_tag="WIB82_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ3842313.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            1321..2838
                     /gene="fbpB"
                     /locus_tag="WIB82_001002"
     CDS             1321..2838
                     /gene="fbpB"
                     /locus_tag="WIB82_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ3842314.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            2859..3917
                     /gene="fbpC"
                     /locus_tag="WIB82_001003"
     CDS             2859..3917
                     /gene="fbpC"
                     /locus_tag="WIB82_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ3842315.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGSALFFPGNTL"
     gene            4102..5604
                     /gene="pta"
                     /locus_tag="WIB82_001004"
     CDS             4102..5604
                     /gene="pta"
                     /locus_tag="WIB82_001004"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ3842316.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFSRQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            5735..6373
                     /gene="hisH"
                     /locus_tag="WIB82_001005"
     CDS             5735..6373
                     /gene="hisH"
                     /locus_tag="WIB82_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ3842317.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            6406..7143
                     /gene="hisA"
                     /locus_tag="WIB82_001006"
     CDS             6406..7143
                     /gene="hisA"
                     /locus_tag="WIB82_001006"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ3842318.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVARDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            7156..7923
                     /gene="hisF"
                     /locus_tag="WIB82_001007"
     CDS             7156..7923
                     /gene="hisF"
                     /locus_tag="WIB82_001007"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ3842319.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            7954..8349
                     /gene="hisI"
                     /locus_tag="WIB82_001008"
     CDS             7954..8349
                     /gene="hisI"
                     /locus_tag="WIB82_001008"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ3842320.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            8456..10051
                     /locus_tag="WIB82_001009"
     CDS             8456..10051
                     /locus_tag="WIB82_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ3842321.1"
                     /translation="MFQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPISMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            10070..10258
                     /locus_tag="WIB82_001010"
     CDS             10070..10258
                     /locus_tag="WIB82_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
                     /protein_id="HEZ3842322.1"
                     /translation="MPSKPEKRASDGIFACNSNADGQNQGASDTVIGHPVRMNLGLTQ
                     ERWPDIVFHETREHSVKL"
     gene            10277..10410
                     /locus_tag="WIB82_001011"
                     /pseudo
     CDS             10277..10410
                     /locus_tag="WIB82_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
     gene            complement(10427..10957)
                     /locus_tag="WIB82_001012"
     CDS             complement(10427..10957)
                     /locus_tag="WIB82_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ3842323.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGERSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(10998..>11798)
                     /locus_tag="WIB82_001013"
     CDS             complement(10998..>11798)
                     /locus_tag="WIB82_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3842324.1"
                     /translation="RTPNIKYIYQDNQGLSEARNTGIKNSNGKYIVFIDSDDFINCQV
                     LLDFLSKDDIDMPDVVFLNAVKYDKGSVSYFGEDYQPEKILNQSKVEVLKGLCRFRKF
                     PGSAWNKIIKRELIIKEKLFFEKGIYSEDIEWSMRLFNAATTFFYLDGCYYYYRQGRK
                     DSITGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIECD
                     ADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKVIRGNV"
BASE COUNT         2906 a         3580 c         2799 g         2513 t
ORIGIN      
        1 gttttaaaga gaggggttct caagcattcc gacaaatcaa aatgccgcct ttacccacat
       61 tccgccacgc attgcccgcc acttccgcct tacgggcgag actcacggaa aaaatacaag
      121 tcagaacaaa caataaccgg atcggacaac aacaaattta aaaaataatt atttgcattt
      181 ataaaaaaga aggaatacac tacacaccgt tatgtcttaa caaggtatac actctcattc
      241 attaggagaa aatcgatatg aaaacatcta tccgatacgc actgcttgcc gcagccctga
      301 ccgccgcaac ccccgcgctg gcagacatta ccgtgtacaa cggacaacac aaagaagcag
      361 cacaagccgt tgcagatgcc tttacccggg ctaccggcat caaagtcaaa ctcaacagcg
      421 ccaaaggcga ccagcttgcc ggtcaaatca aagaagaagg cagccgaagc cccgccgacg
      481 tattctattc cgaacaaatc ccggcactcg ccaccctttc cgcagccaac ctcctagagc
      541 ccctgcccgc ctccaccatc aacgaaacac gcggcaaggg cgtgccggtt gccgccaaaa
      601 aagactgggt ggcactgagc ggacgttcgc gcgtcgtcgt ttacgacacc cgcaaactgt
      661 ctgaaaaaga tttagaaaaa tccgtcctga attacgccac gccgaaatgg aaaaaccgca
      721 tcggttacgc ccccacttcc ggcgcgttct tggaacaggt tgtcgccatc gtcaaactga
      781 aaggcgaagc ggccgcattg aaatggctca aaggtctgaa agaatacggc aagccttacg
      841 ctaaaaactc cgtcgccctt caagcggttg aaaacggcga aatcgatgcc gccctcatca
      901 acaactacta ctggcacgct ttcgcgcgtg aaaaaggcgt acaaaacgtc cacacccgcc
      961 tgaatttcgt ccgccacaga gatcccggcg cactcattac ctattccggt gcagccgtgt
     1021 taaaatcctc ccaaaacaag gatgaggcga aaaaattcgt cgccttcctc gccggcaagg
     1081 aaggacagcg tgccctgacc gccgtccgtg ccgagtatcc tttgaatccg cacgtggtat
     1141 ccaccttcaa tttggaaccc atcgccaagt tggaagcacc ccaagtgtcc gccaccactg
     1201 tttccgaaaa agaacacgcc acccggctgc ttgagcaagc cggtatgaaa taagccgttt
     1261 tcggattgtc aaacgggtgg acatttatac tgtccgcccg ttttgccgat aaaaaacact
     1321 atgtctccta aaaaaatacc catttggctt accggcctca tcctactgat tgccctaccg
     1381 cttaccctgc cttttttata tgtcgctatg cgttcgtggc aggtcggcat caaccgcgcc
     1441 gtcgaactac tgttccgccc gcgtatgtgg gatttgctct ccaacacctt gacgatgatg
     1501 gcgggcgtta ccctgatttc cattgttttg ggcattgcct gcgccctttt gttccaacgt
     1561 taccgcttct tcggcaaaac cttttttcag acggcaatca ccctgccttt gtgcatcccc
     1621 gcatttgtca gctgtttcac ctggatcagc ctgaccttcc gtgtcgaagg cttttggggg
     1681 acagtgatga ttatgagcct gtcctcgttc ccgctcgcct acctgcccgt cgaggcggca
     1741 ctcaaacgca tcagcctgtc ttacgaagaa gtcagcctgt ccttgggcaa aagccgcctg
     1801 caaacctttt tttccgccat cctcccccag ctcaaacccg ccatcggcag cagcgtgtta
     1861 ctgattgccc tgcatatgct ggtcgaattt ggcgcggtat ccattttgaa ctaccccact
     1921 tttaccaccg ccattttcca agaatacgaa atgtcctaca acaacaatac cgccgccctg
     1981 ctttccgctg ttttaatggc ggtgtgcggc atcgtcgtat ttggagaaag catatttcgc
     2041 ggcaaagcca agatttacca cagcggcaaa ggcgttgccc gtccttatcc cgtcaaaacc
     2101 ctcaaactgc ccggtcagat tggcgcgatt gtttttttaa gcagcttgtt gactttgggc
     2161 attattatcc cctttggcgt attgatacat tggatgatgg tcggcacttc cggcacattc
     2221 gcgctcgtat ccgtatttga tgcctttatc cgttccttaa gcgtatcggc tttaggtgcg
     2281 attttgacta tattatgtgc cttgcccctt gtttgggcat cggttcgcta tcgcaatttt
     2341 ttaaccgttt ggatagacag gctgccgttt ttactgcacg ccgtccccgg tttggttatc
     2401 gccctatcct tggtttattt cagcatcaac tacacccctg ccgtttacca aacctttatc
     2461 gtcgtcatcc ttgcctattt catgctttac ctgccgatgg cgcaaaccac cctgaggact
     2521 tccttggaac aactccccaa agggatggaa caggtcggcg caacattggg gcgcggacac
     2581 ttctttattt tcaggacgtt ggtactgccg tccatcctgc ccggcattac cgccgcattc
     2641 gcactcgtct tcctcaaact gatgaaagag ctgaccgcca ccctgctgct gaccaccgac
     2701 gatgtccaca cactctccac cgccgtttgg gaatacacat cggacgcaca atacgccgcc
     2761 gccacccctt acgcgctgat gctggtatta ttttccggca tccccgtatt cctgctgaag
     2821 aaatacgcct tcaaataaca gcttgaggaa gtaccgccat gaccgccgcc ctgcacatcg
     2881 gacacctgtc caaaagtttt caaaacaccc cggttttaaa cgacatttcg ctcagcctcg
     2941 acccgggcga aatcctcttt atcgtcggcg cgtccggctg cggcaaaacc acccttttac
     3001 gctgccttgc cggttttgaa caacccgatt ttggagaaat ttcgctttcc ggcagaacca
     3061 tcttctcgaa aaataccaac ctccccgtcc gcgaacgccg tttgggttat gtcgtacagg
     3121 aaggtgtact gtttccccac ctgaccgttt accgcaacac cgcctacggg ctgggcaacg
     3181 ggaaaggcaa gacggcgcaa gaacggcagc gcatcgaagc tatgttggaa ttgaccggca
     3241 tttccgaact tgccggacgc tatccgcacg aactttcggg cggacagcaa cagcgcgtcg
     3301 ccctcgcccg cgccctcgca cccgatcccg aactgatttt gttggacgaa cccttcagcg
     3361 cgctggacga acagttgcgc cgccagattc gcgaagacat gattgccgcc ctgcgcgcca
     3421 acggcaaatc tgccgttttc gtcagccacg accgcgaaga agccctgcaa tacgccgacc
     3481 ggattgccgt gatgaaacag gggcgcatcc tccaaactgc aagccctcac gaattgtacc
     3541 gacaacctgc cgaccttgat gccgccctgt ttatcggcga aggcatcgtg ttccccgccg
     3601 cgctcaacgc cgacggcacc gccgattgcg gattgggccg cctgcccgtt caaagcggcg
     3661 cacccgcagg cacacgcggc acactgctca tccgtccgga acagttcagc cttcaccccc
     3721 attccgcacc cgccgcctcc attcacgccg tggttctcaa aaccacgccc aaagcgcggc
     3781 ataccgaaat cagcctccgg gtcggacaaa ccgtcctcac gctcaacctc ccttccgccc
     3841 ccaccctgtc agacggcatt tccgccgtcc tccatttgga cggttccgcc ctgttcttcc
     3901 ccggaaatac cctctgaaag gcggcagcat ccacaagcct gcggatattt atcttgttgg
     3961 aaacagaatt tgtttgctat attcaacctg cgcgcctcaa gccaaacacg gcacgcacgg
     4021 cacgcaggca gccgtttctg cctatatgcc gccccttcca accacatatg ccgcacaccg
     4081 cagcatacga aaggatatat catggcaaaa gtactcatcg tacccgtatc tgccggactg
     4141 gacgcctccg ccgccgcaca agcctttgca aaagcactgg acgcacaaat tttccaagcc
     4201 gttgacgcaa ccgccgaaac cctgctcgcg caaggcaaaa gcgatgactg gttcgacgca
     4261 ctggtcggca aagttgccgc actcgatgcc gccaacctcg tcatcgaagg catcgcgccc
     4321 gatgccgaca aaatctacct cgcaggcaaa aacgtcgaac tggcattgtc ccttgatgcg
     4381 gcagccgtct tcgccgtccg ttccgacaac gccgatgccg acgaactggc aaatcggctg
     4441 aaccttgcca aacagttctt cgccgccgcg ccgggcgtat tggaaggttt tgtcgtggac
     4501 ggcgcggcag cctccgttgc cgaagcggca gccgaaaaaa ccggcctgac cttcttcggt
     4561 tcgagcgacg cgctgaaaga cgtatccgta ttggcaggcc gcgaagcaaa acgcctgtcg
     4621 ccggcgcaat tccgctacaa cctgatcgac ttctcccgcc aagccgacaa acgcatcgtc
     4681 ctgcctgagg gcgcagaacc ccgcaccgtc caagccgccg ccatctgcca cgaaaaaggc
     4741 attgcccgct gcgtcctgct tgccaaacgc gaagaagtcg aagccgttgc caaagaacgc
     4801 ggcatcagcc tgcccgactc tttggaaatc atcgatcccg cctcattggt cgaacaatac
     4861 gtcgagccaa tgtgcgaact gcgcaaatcc aaaggcctga cacccgaaga cgcgcgcaag
     4921 caactgcaag acaccgtggt actcggtacg atgatgatgg cgcaaaatga tgtggacggt
     4981 ttggtatccg gtgcggttca caccaccgcc aacaccatcc gccccgcttt gcaactgatt
     5041 aaaaccgcac cgggcgcaag cctcgtgtcc agcgtattct ttatgctgct gcccaaccaa
     5101 gtcctcgtct tcggcgactg cgcggttaat ccgaacccga ccgcgcaaca gcttgccgac
     5161 atcgccatcc agtctgccga ttccgcaaaa gccttcggca tcgacccgaa agtggcgatg
     5221 atttcctact ccaccgtcaa ctccggcagc ggccccgatg tcgataccgt catcgaagca
     5281 accaaacttg cccgggaaaa acgccccgac ctcgccatcg acggcccgct gcaatatgat
     5341 gcggcaaccg tgccgggcgt gggcaaatcc aaagccccgg gcagcccggt ggcaggacag
     5401 gcaaccgttt tggtcttccc cgacctgaac accggcaact gcacctataa agccgtccaa
     5461 cgcagtgcca acgtcttgag cgtcggcccg ctgctgcaag gcctgcgcaa accggtcaac
     5521 gacctgtcgc gcggcgcgct ggtggaagac atcgtgttca ccatcgccct gactgccgtt
     5581 caggcaaaac aaatggaagg ctgacaaacg gctttccggg tttgaaccct atgccgtctg
     5641 aagacagatt cccgttttca gacggcattt ttatcagccc ggcacatttg tttgttaaaa
     5701 tcgcagccat attgcaaaaa aagaggagga aaccatgcaa accgccatta tcgattacgg
     5761 tatgggcaac ctgcattccg tattgaaatc cgtccggacg gcggggcagc ttgccggaaa
     5821 aaataccgaa atctttttaa gcggcgaccc cgaacgcgtg tcccgcgccg acaaagtcat
     5881 ttttcccggg cagggcgcga tgcccgactg tatggcggca ctgacgcgcg gcggcctgga
     5941 cgaggcagtc aaagatgcct taaaaaacaa accctttttc ggaatctgcg tcggcgcgca
     6001 acttttattc gaccacagcg aagaagggaa caccgacggc ttgggctggt tcggcggcaa
     6061 agtcagacgc tttgagcgcg acctccgcga cccgcaggga tgccgtctga aagtcccgca
     6121 tatgggctgg aacaccgtgc gccaaaccca aaaccacccg ctgtttaaag atattcccca
     6181 agacacgcgt ttttacttcg tccacagcta ctatttcgcc cccgaaaatc ccgaaaccat
     6241 attgggcgaa agcgactacc cgtccccgtt tgcctgcatc gtcggcaaag acaacgtatt
     6301 cgccacgcaa tttcacaccg aaaaaagcca cgatgccggg ctgacgatgt tgaaaaactt
     6361 tttaaactgg taagccggac acggccccgc acaaggaaaa aaactatgct gctgataccc
     6421 gccatcgatt tgaaagaagg acgctgcgtc cgcctgaaac aagggctgat ggaagaggcg
     6481 accgtctttt ccgattcgcc cgccgaaacc gcgctgcact ggttcaaaca gggcgcgcgc
     6541 cgcctgcatc tggtagattt gaacggcgcg tttgccggtg ttccgcaaaa cctgcccgcc
     6601 atcaaagaca tccttgccgc cgtcgcccga gacatccccg tacagctcgg cggcggcata
     6661 cgcgatttga aaaccatcgg acaatatttg gatttgggct taaacgacgt gattatcggc
     6721 acggcggcgg tcaaaaaccc cgacttcgtg cgcgaggcgt gcaaagcctt ccccggcagg
     6781 attatcgtcg gactggatgc caaagacggt atggccgcca tcgacggctg ggcaaccgta
     6841 accgggcatc atgtaattga tttggcaaaa cgctttgaag acgacggtgt caacagcatc
     6901 atctacaccg acatcgggcg cgacggtatg atgagcggcg tgaacatcga cgcgacggtc
     6961 aaactcgccc aagccgtccg cattcccgtc atcgcctccg gcggactgac cggcttggac
     7021 gacatccgcg ccctgtgtgc cgccgaaaaa catggcgtag caggcgcgat taccggccgc
     7081 gcgatttacg agggcagcat cgattttgcc caagcgcagc aactggcaga ttccctcgac
     7141 taaaggcatc cgattatggc actggcaaaa cgcatcatcc cctgtctcga cgtaaaagac
     7201 gggcgcgtcg tcaaaggcgt gaacttcatc ggtttgcgcg acgcgggcga ccctgtcgaa
     7261 gccgccaaac gctacaacgg cgaaggcgcg gacgaattga ccttcctcga catcaccgcc
     7321 tcatccgaca accgcgacac catcctgcac atcatcgaag aggttgccgg acaagtcttc
     7381 atccccctga ccgtcggcgg cggcgtacgc accgttgccg acatccgccg cctgctcaat
     7441 gccggcgcgg acaaagtcag catcaacacc gccgccgtta cccgtcccga cctgattgac
     7501 gaagccgccg gatttttcgg ttcgcaagcc atcgtcgccg ccgtcgatgc caaagccgtc
     7561 aactccgaaa acacacgctg ggaaatcttt acccacggcg ggcgcaatcc gaccggtttg
     7621 gatgcggtgg aatgggcgat cgaaatgcaa aaacgcggcg cgggcgaaat cctgcttacc
     7681 ggtatggaca gggacggtac gaaacagggt ttcaacctgc cgctgacccg cgccgttgcc
     7741 gaagccgtcg acatccccgt catcgcctcc ggcggggtcg gcaatgtccg gcacctgatt
     7801 gaaggcataa ccgaaggcaa agccgatgcc gtacttgccg ccggcatttt ccatttcggg
     7861 gaaatcgcca tccgcgaagc caaacgcgct atgcgcgaag ccggcatcga agtgcgcctc
     7921 tgaccgcccc gactatgccg tctgaaagga aatatggata aaaacctgct tgaagccgtc
     7981 aaatttgacg aaaaaggttt ggtttgcgcc atcgcccaag atgccgaaac caaacgtgtt
     8041 ttaatggtgg cgtggatgaa cgccgaagcc ctgcaaaaaa ccgtcgaaac cggctttgcc
     8101 cactattaca gccgttcgcg ccaaaaacaa tggatgaagg gcgaagagtc gggacacacg
     8161 caaaaagtcc gcgaactgcg cctcgactgc gacggcgaca ccattgtgat gctcatcgcc
     8221 caaaacggcg gcatcgcctg ccacaccggg cgagaaagct gcttttacaa agtctggcgt
     8281 ggcagcgcgt gggaaaccgc cgatgccgtc ctgaaagacg aaaaagagat ttacggcagc
     8341 acgcactgac cgcctccaac attgaattat caggcatttt tttgtacaat ttcgccgtct
     8401 caaacactgt ccgggccgtc tgaaaagcgg cctgaacctt tttgcaaaga aaaccatgtt
     8461 ccaagaaatc ctcgaccaag tgcgccgccg ccgcacgttt gccatcatct cccaccctga
     8521 cgcaggtaaa accacgttga ctgaaaaact cttgctgttt tcaggtgcga ttcaaagcgc
     8581 gggtacggta aaaggcaaaa aaaccggcaa attcgccact tccgactgga tggacatcga
     8641 gaagcagcgc ggcatttccg tggcatcaag cgtgatgcag ttcgactata aagaccacac
     8701 cgtcaacctc ttggacacgc cgggacacca agatttctca gaagacacct accgcgtttt
     8761 gaccgccgtg gacagcgcgt tgatggtcat cgacgcggca aaaggcgtgg aagcgcaaac
     8821 catcaagctc ttaaacgtct gccgcctgcg cgatacgccg attgttacgt tcatgaacaa
     8881 atacgaccga gaagtacgcg attcgctgga actcttggac gaagtggaaa acatcctgca
     8941 aatccgctgc gcgcccgtta cctggccgat tagtatgggc aaaaacttca aaggcgtata
     9001 ccacatcctg aacgatgaaa tctatctctt tgaagccggc ggcgaacgtc tgccgcacga
     9061 attcgacatc atcaaaggta tcgataatcc tgaattggaa caacgctttc cgttggaaat
     9121 ccaacagttg cgcgacgaaa tcgaattagt gcaggcagca tcgaacgagt ttaatctcga
     9181 cgaattcctt gcaggcgaac tcacgcccgt gtttttcggt tcggcgatta acaacttcgg
     9241 tattcaggaa atcctcaatt cattgattga ctgggcgccc gcgccgaaac cgcgcgacgc
     9301 gaccgtacgt atggtcgagc cggacgagcc gaagttttcc ggatttatct tcaaaatcca
     9361 agccaatatg gacccgaaac accgcgaccg tattgccttc ttgcgcgtct gctccggcaa
     9421 attcgagcgc ggcatgaaga tgaaacacct gcgtatcaac cgcgaaatcg ccgcctccag
     9481 cgtggttacc ttcatgtcgc acgaccgcga gctggttgaa gaagcctacg ccggcgacat
     9541 tatcggcatc ccgaaccacg gcaacatcca aatcggcgac agcttctccg aaggcgaaca
     9601 actggcgttc accggcatcc cattcttcgc acccgaactg ttccgcagcg tacgcatcaa
     9661 aaacccgctg aaaatcaaac aactgcaaaa aggcttgcaa cagctcggcg aagaaggcgc
     9721 ggtgcaggtg ttcaaaccga tgagcggcgc ggatttgatt ttgggcgcgg tcggcgtgtt
     9781 gcagtttgaa gtcgttacct cgcgccttgc caacgaatac ggcgtggaag ccgtgttcga
     9841 cagcgcatcc atctggtcgg cgcgctgggt atcgtgcgac gacaagaaaa aactggcgga
     9901 atttgaaaaa gccaacgcgg gcaacctcgc catcgacgcg ggcggcaacc tcgcctacct
     9961 cgcccccaac cgcgtgaatt tgggactcac gcaagaacgc tggccggaca tcgtgttcca
    10021 cgaaacgcgc gagcattcgg tcaaacttta aaaagcaatc ggcaataaaa tgccgtctaa
    10081 acccgaaaaa agggcttcag acggcatttt tgcctgcaac tcaaacgcag acggtcaaaa
    10141 tcaaggcgca tcggataccg ttatcgggca tcccgtccgc atgaatttag ggctgacgca
    10201 agaacgttgg ccagacatcg tgttccacga aacgcgcgag cattcggtca aactttaaaa
    10261 agcaatcggc gataaaatgc cgtctgaacc cgaaaaaagg gcttcagacg gcatttttgc
    10321 ctgcaactca aacgcagacg atcaaaatca aggcgcatcg gataccgtta tcggatgcgt
    10381 cccgtccgcg tgaatttggg gctgacgtag gaacgccgat gtgatttcac atcccgtact
    10441 gtttcgacag cttcacataa tgcgcggcgg aatatttcaa aaaggctttt tcctcatcgg
    10501 tcagcacgcg cacttgtctg accggcgaac cgacataaag atagccaccc tccaaacgtt
    10561 tacgcggcgg aacgaggctg cccgcgccaa tcatcacttc gtcctcaatc acagcatcgt
    10621 ccaaaacggt gctgcccata ccgaccaaga cgcggttgcc gattcggcag ccgtgcagca
    10681 tcactttgtg ccccacggta acgtcttcgc cgataaccag cggcgaccct tcgggtttgg
    10741 cggcggtttt gtgggaaacg tgcaggacgc tgccgtcctg tatattgctg cgctcgccga
    10801 cggtgatgct gttcacatcg ccgcgcaaca cggcgcacgg ccacacggaa acgtcttcgg
    10861 caagcgacac ttcgccgatg acgacgcacg cttcgtctat catacagctt tcgtggattt
    10921 cgggcgtgcg gttttggaaa gttcggattg cgttcatttt tcctccttcg gtaagatgta
    10981 tagtgttaaa ggatttatta aacattccct ctgattactt ttaaaatcct gcctgtaata
    11041 tcgaccccga ataatgtgat tatcgggaat atcagcttat atatcaattt attggacttt
    11101 aacagcataa accttaaatg atacgccctt ctttttatat cagcatcaca ctctatattt
    11161 ttactcgtca tgataaaaag caaaacgaga tattcgtagg aaagaaagga ataaagataa
    11221 cttgatatat ccctattaaa ttccatttcc gcatttttct ccaaaatata taataatgac
    11281 tttatacttt tttccgaaac agttcccgta atagaatctt ttcttccctg ccgataatag
    11341 taataacaac cgtccaaata aaaaaaagtt gttgccgcat taaataacct cattgaccat
    11401 tcgatatctt cagaataaat ccccttttca aaaaatagtt tttctttgat gatcaattct
    11461 ctttttataa tcttattcca cgccgaaccc ggaaattttc taaatcggca taatcctttc
    11521 aaaacttcga ctttggattg attgagtatt ttttcaggct gataatcttc gccaaaatat
    11581 gaaacacttc ccttatcata tttaaccgca tttaaaaaaa ccacatccgg catatcaata
    11641 tcatctttac taagaaaatc cagcaagacc tgacaattaa taaaatcatc cgaatcaata
    11701 aagactatat attttccgtt tgaatttttt attccggtat ttctcgcctc tgacaatcct
    11761 tggttatcct gatatatata tttgatattt ggtgtccg
//
LOCUS       DAWHRS010000053        11676 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWHRS010000053 DAWHRS010000000
VERSION     DAWHRS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.053"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            499..1800
                     /gene="bioA"
                     /locus_tag="WIB82_001014"
     CDS             499..1800
                     /gene="bioA"
                     /locus_tag="WIB82_001014"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ3842325.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1797..2444
                     /gene="bioD"
                     /locus_tag="WIB82_001015"
     CDS             1797..2444
                     /gene="bioD"
                     /locus_tag="WIB82_001015"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ3842326.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2411..2641
                     /locus_tag="WIB82_001016"
     CDS             2411..2641
                     /locus_tag="WIB82_001016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842327.1"
                     /translation="MDGVGKNRRGIKIGKNMEHLYDEYGNTTPNRRTTNKKDETISSK
                     VTVAAIPVAAPFAIADIVDQDFIQAIMKITGH"
     gene            2665..3051
                     /locus_tag="WIB82_001017"
     CDS             2665..3051
                     /locus_tag="WIB82_001017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842328.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            3045..3488
                     /locus_tag="WIB82_001018"
     CDS             3045..3488
                     /locus_tag="WIB82_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842329.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            3670..3942
                     /locus_tag="WIB82_001019"
     CDS             3670..3942
                     /locus_tag="WIB82_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842330.1"
                     /translation="MTIRANNDIEYFVKNNTTNEQRIDDFRSCLSVACPEIYKLYQLP
                     DEKGNYQYIIKKYYDNKYNDNAVKSTNCIGDNARCMLNNKGYSTKW"
     gene            3945..4421
                     /locus_tag="WIB82_001020"
     CDS             3945..4421
                     /locus_tag="WIB82_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ3842331.1"
                     /translation="MGYGFEEWLPDLPAPVSDGIGLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            4451..5341
                     /gene="ubiA"
                     /locus_tag="WIB82_001021"
     CDS             4451..5341
                     /gene="ubiA"
                     /locus_tag="WIB82_001021"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ3842332.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            5526..5975
                     /gene="ptsN"
                     /locus_tag="WIB82_001022"
     CDS             5526..5975
                     /gene="ptsN"
                     /locus_tag="WIB82_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ3842333.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            5977..6939
                     /locus_tag="WIB82_001023"
     CDS             5977..6939
                     /locus_tag="WIB82_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ3842334.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQHNENRPD"
     gene            6920..7774
                     /gene="rapZ"
                     /locus_tag="WIB82_001024"
     CDS             6920..7774
                     /gene="rapZ"
                     /locus_tag="WIB82_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ3842335.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPKLRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            7855..8796
                     /gene="ylqF"
                     /locus_tag="WIB82_001025"
     CDS             7855..8796
                     /gene="ylqF"
                     /locus_tag="WIB82_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ3842336.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            8897..9499
                     /locus_tag="WIB82_001026"
     CDS             8897..9499
                     /locus_tag="WIB82_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ3842337.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            9683..11356
                     /gene="recN"
                     /locus_tag="WIB82_001027"
     CDS             9683..11356
                     /gene="recN"
                     /locus_tag="WIB82_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ3842338.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPTLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRSLGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDKIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            11466..11542
                     /locus_tag="WIB82_001028"
     tRNA            11466..11542
                     /locus_tag="WIB82_001028"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11500..11502,aa:Arg,seq:acg)
     gene            11560..11634
                     /locus_tag="WIB82_001029"
     tRNA            11560..11634
                     /locus_tag="WIB82_001029"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11593..11595,aa:Glu,seq:ttc)
BASE COUNT         3018 a         2750 c         3038 g         2870 t
ORIGIN      
        1 ggctgtaacc ttcaatcgga atttgttgcc tgcgggatat gcggtatgaa tgtttgggat
       61 atatggggca ggatggtgga aatctatcat aagtataaga agccgtgcct ggttttggcg
      121 gtggattttg tgatgggtat ggtattcata gagccgaatg aggagctgtg catcggtaga
      181 tgctatgcgc ctatgtcgga gtcccctgat tttgctaacg ctgttgcgat ggctgttgct
      241 atgatctgta tcgtatggat tgccgttttg tctatgactg attgaagcgt tgggcggagg
      301 ctgtgtgaaa caatattggg cgttgggtcg tctgattcca atcgggcttg gggaatgcga
      361 aacggtgtgc gcttatactg cggacgattt gttgcgtggt tttacgcccg aaacggatgg
      421 aaaggtgtgg gaagcggtct gtcggagtag aatacgcgtt ttgcgtttga atgcagcaat
      481 aagaaaagag agaaacttat gccgtctgaa catcaacaca catcatcatt gcttgatttc
      541 gaccgtaccc atctgctcca tccctatact tccatgaccg atccgcttcc cgtttatcct
      601 gtcaaacgtg cagaaggggt gtttatcgaa ttggcggacg gcacgcggct gattgacggg
      661 atgtcgtctt ggtggtgtgc gatacacggt tacaatcatc ccgttttgaa tcaggcggtt
      721 gagacgcaga tgaaacagat gtcgcatgtg atgttcggcg gtttgacgca tgagccggcg
      781 gtggagctgg gcaaattgtt ggtcgggatt ttgccgcagg ggctggatcg tattttttat
      841 gcggattcgg gttcggtttc ggtggaagtc gcgttgaaga tggcggtgca ataccagcag
      901 gcgcggggtt tgcgtgcaaa acaaaatatg gcgacggtgc ggcgcgggta tcacggcgat
      961 acttggaacg cgatgtccgt ctgcgatccg gaaacgggga tgcaccatat tttcggcagc
     1021 gcgttgccgc agcgttattt tgtcgataac ctgacaagcc gtttcgacga tgaatgggac
     1081 gaggcggatt tgcagcctgt ccgcgccttg tttgaagcgc atcatacgga cattgccgcc
     1141 tttattttgg aaccggtcgt gcagggcgcg ggcggtatgt atttttatca tccgcagtat
     1201 cttcgcggat tgcgcgattt gtgcgacgag tttgatatcg ttttgatttt tgatgaaatc
     1261 gccaccggat tcgggcgcac gggcaagatg tttgcctgcg aacacgcgga ggtcgtgccg
     1321 gatattatgt gcatcggcaa gggtttgagc ggcggctata tgacgctggc ggcagcaatc
     1381 acttcgcaaa aagttaccga aacgatttcg cgcggcgaag cgggcgtgtt catgcacggc
     1441 ccgacattta tggcgaaccc gctggcgtgt gccgttgcct gcgcttcggt caaactgctt
     1501 ttgtcccaag attggcaggc aaatatccgc cgcatcgaaa gcatcttaaa atgccgtctg
     1561 aaaaccgcgt gggacattcg tggtgtgaaa gacgtgcgcg ttttgggcgc catcggcgtg
     1621 atcgagttgg aaaaaggcgt ggatatggcg cgttttcaag cggattgcgt ggcgcagggc
     1681 atttgggtgc gcccgttcgg caggttggtg tatctgatgc cgccttacat catttcagac
     1741 ggcgttttga ccaaacttgc cgacaaaacc gtgcaaatct tgaaggaaca cagcaaatga
     1801 aaggcgttta cttcgtcagc ggcatagaca cggacatcgg caaaaccgtc gccaccggcg
     1861 tgttggcaaa acaattgttg cagcagggca aaagcgtgat tacgcaaaag cccgtgcaaa
     1921 ccggttgcca aaacattgcc gacgacatcg ccgtccatcg taaaatcatg ggtaaaccca
     1981 tgcaggaagc cgacaaacaa gggctgacca tgcctgaaat cttcagttat ccagcttcgc
     2041 ctcatctcgc tgcccgtctt gacggcaggg ctttggactt ggacaaaatc cgcaccgcca
     2101 cacaagaatt ggcggcgcag tacgaagtcg ttttggtcga aggcgcgggc ggattgatgg
     2161 ttccgctgac ggaaaacctg ttaaccattg attatatccg tcagcaaggc tatcccgtca
     2221 tcctcgttac cagcggacgg cttggcagta tcaaccacac tttactcagt ttcgccgcgc
     2281 tcaaacaata cggcattcgc ttgtacagcc tcgtgttcaa ccacatccac gacagccgcg
     2341 acgcacacat cgcccaagac agcctgagct acttgaaatg ccgtctgaaa gccgattttt
     2401 ccgaagcgga atggatggag ttggcaaaaa cagacgcggt ataaagattg ggaaaaatat
     2461 ggaacaccta tatgatgaat atggaaatac aacgccaaat agaagaacaa caaataaaaa
     2521 agatgaaact atttcaagta aagtaactgt tgcagcgata ccagtagccg ctccatttgc
     2581 tattgctgat atagtcgatc aggattttat ccaagcgatt atgaaaatta ctggtcattg
     2641 attatctata tattggtggt tgttatgaaa tgctatatta aagttgtcat tattgttttt
     2701 ataatggtaa tgacaacttc ttgtaatcct tgcttttttg caacttcttc ttgtgattat
     2761 tcttttagaa aaaactatcc tataaatagc gacgatgaat attatgttaa gaataatacg
     2821 agcaaagaaa caagaattgc ggatttacgc tattgtattt cttatgcatg tccagaagtt
     2881 tatcaaaaat atcaactacc agatgaaaat ggagattata gggagattat tcgtagaaaa
     2941 tataaagatg attataaaaa gggctataag aatgcagatg atgtcatgac atgctacgga
     3001 aagattggag aatgcatgaa aaataataaa ggatattcaa caaaatggta atgttccgct
     3061 tattatcaac agcccttgta ttgttgataa tgcagggctg ttattacaca actattgata
     3121 atgaaaaatg ctttgttgcc ccacaaagcg ttcattgtcc tgtaagttct gatattgaaa
     3181 atgttattag aaaaaatcct aataaaagca taaatcaagt taggagtgat attaaatatt
     3241 gtgctaatat gtatttttcc aatatacggg aattaaatat tccgcaagat tttgataatt
     3301 acattttcat gcttaaagaa aaaggtattc caagaaatga taaggaattg gcttataatg
     3361 cagttagaaa tatgcacatt atttgtttga gttctttggg tggttatgat tattatcaaa
     3421 aaaccgcaga atttcagaaa atagattata aaaaagaaat ggagaaattg ttaaatgaaa
     3481 accattaaat ttgctattgc cgatatagtt gatagcgact ttatccaagt gattatgaaa
     3541 ttaagcgggc attgatttat gattacgcgt tgttttaaag ttgtcattat tgtttttata
     3601 atggtaatga caacttcttg taatccttgc ttttttgcaa cttcgtcttg taattatcct
     3661 tttgatgata tgacgataag agcaaacaac gatattgaat attttgttaa aaataatacg
     3721 actaacgaac aacgaattga tgatttcaga agttgccttt ccgttgcttg tcctgaaata
     3781 tataaattat atcaattacc tgatgaaaag ggtaattatc aatatattat aaaaaaatat
     3841 tatgataata aatacaatga taatgcagtc aaatcgacaa attgtattgg ggataatgca
     3901 agatgtatgc ttaataataa aggatattca acaaaatggt aataatgggt tacggctttg
     3961 aggaatggct gcccgacctg cccgcccctg tttcagacgg catcggcctg ccgatgagcc
     4021 ggttattgaa agcccggtcg ctgaccgccg cattgcgcgc cttgccgcat acattttcgg
     4081 tggaactgct gaaactgggc gaattggaga cggaatgcgg agggaggctg gtgcgcgaag
     4141 ttttgttgaa gctggacggt atccctgttg tcgcggcaag gagcgagtgc cgtatcggtt
     4201 cggcgttttg gcaaaacatt ttggactgcg gcacgcgtcc tttgggcgag cgtctgtttc
     4261 aagccgattt ggaaggggcg cgttcggcgt ttgagtttgc cgtttccggc gaaggatgcg
     4321 gacggtactt tgccgcgcgg cgttctcggt tttcccgtca cggcgaggaa atgctgctga
     4381 ccgagtattt tctgcccgaa ctgaaacgct ttatcggata aaataccgtt ttttcaagct
     4441 gtgcggcaat atgaatccta aatccccttt atttttacga ctgtccgatc gtttggatgt
     4501 gtacctgcgc ctgatgcggg cggacaagcc cattgggacg ctgcttttgc tgtggccgac
     4561 ctactgggca ttgtggctgg cttcagacgg cattcccgat ttggcggtat tggcggcgtt
     4621 tacaatcggc acgtttttaa tgcggagtgc cggttgcgtc atcaacgatt ttgccgaccg
     4681 cgattttgac ggtgctgtcg agcgcaccaa aaaccgtccg ttcgcacagg gtcgggtcgg
     4741 aaaaaaagaa gcgctgctgc tgacggcatt tttgtgcctg cttgccgcat tgtgcctgat
     4801 tccgctgaat catctgacat ggctgatgag cctgcccgcg ctgtttcttg cgctgactta
     4861 cccgtttacc aaacgctttt ttccgattcc ccaactctat ctcgggcttg ccttttcctt
     4921 cggtatcccg atggcgtttg ccgccgttgc cggaaacgtg ccgcctcaag cgtggatact
     4981 ctttgccgcc aatgtgttat ggactctggc gtatgacacg gtttatgcaa tggcggacaa
     5041 agaagacgat ttgaaaatcg gcatcaaaac ctccgccgtc acgttcgggc gttacgacat
     5101 cgccgccgtt atgctgtgtc atggcggctt taccctgctg atggcagtat tgggtgcggt
     5161 tatcggtgcg gcatgggcat attggacggc aatccccatc gtcctgctgc tgcaataccg
     5221 ccaatatgcc gccatcaaaa gccgcgtccg gcaaatctgt tttgaaacgt ttttggcaaa
     5281 caacagaatt ggttgggtgt ggtttaccgc catttttgcc catacgtttt tcgcgaaata
     5341 aggcagggca atgccgtctg aagagccgta aactgctttg gacggcattt ctatctgtgc
     5401 cgaaaagcgt taaaatatgt ttttaaaacg ctgtgttatg tcagcccgta ccgtatgcgg
     5461 gattgagatt tgccctagca gtcggtacaa tctttctgtt ttgcgatgtc tgaaaagaga
     5521 agcttatgag ccttatcggc gaaattttgc ctttgtccca tattgttttg gatatggagg
     5581 tgggcagtaa aaaaaggctg tttgaggaag cgggcctgct tttggaacgc gaatcctcat
     5641 tgtcccatgc tgatgttttc gaatgtcttt ttgcccgtga aaaactcggt tcgaccggtt
     5701 tggggcaggg cgttgccatc ccgcacggcc gtcatgccgg cgtgaagcag gcgacgggcg
     5761 cgttcatccg cacgcgcgaa cccgtcggat ttgacgcacc ggacggcaag ccggtttccc
     5821 tgatttttat cttgctggtg ccggaaaacg caactggcga acatttggaa gtcttatcca
     5881 aactggccgg caagttttcc caaaaaagca tccgcgaatc gctgatgacg gtttcttctg
     5941 cggaagaagc ccgcgccatt ttgactgaag aataatatgc ccagtatttc cgtccgccga
     6001 ctgtttgacg acaaccaata caagttacaa cttgcctggg ccgccggcaa ttcgggtgcg
     6061 gacaaccgta tcggcgtaga ggcggacaag cccgtcctcg ctttggtcgg acacctgaat
     6121 ttcatccacc ccaaccaaat ccaagtggtc ggtttggcgg aatcggaata tctgaaccgt
     6181 ctcgaatcgg gggaaacggg ttatcagttc ggcgacctgt tcgacatttc tatgtctttg
     6241 gttattgtgg caaacggctt gccggtttcc ccggggctgc gcgactattg ccataaaaac
     6301 gatattccac tgctgacttc caaactcgaa agcccctatc tgatggacgt gttgcggatt
     6361 tacctgcaac gcaccctggc ggcatcgtcc gtcaaacacg gcgtatttct cgatgtgttt
     6421 gaaatcggcg tgctgattac cggacattcc ggcttgggca agagcgaatt ggcgttggaa
     6481 ctgatttcgc gcggccacag cctgattgcc gacgatgcgg tcgagctgtt ccgcatcggt
     6541 ccggaaacat tggaagggcg ttgttcgcct atgctgcgtg attttttgga agtgcgcggc
     6601 ttggggatac tcaatatccg ccatattttc ggcgaaactt ccatccgccc caaaaaaatc
     6661 ctgcaactca ttatcaattt ggtcgaggca gacgacgagt atatgaagca gctcgaccgg
     6721 ttgagtatcc gcaccgaaac cgaatccatc ctcaacgtca acgtccgttc ggttacgctg
     6781 cccgtcgccg tcggacgcaa cctcgccgtt ttggttgagg cggcggtacg caattatatt
     6841 cttcagttgc gcggtaagga tagtacgcgc gaatttttgg aacgtcatca gacgcagctt
     6901 aaagaaaacg agcaacacaa tgaaaatcgt cctgattagc gggttgtccg gttcgggcaa
     6961 atccgtcgca ctgcgccaaa tggaagattc gggttatttc tgcgtggaca atttgccttt
     7021 ggaaatgttg cccgcgctgg tgtcgtatca tatcgagcgt gcggacgaaa ccgaattggc
     7081 ggtcagcgtc gatgtgcgtt ccggcataga catcggacag gcgcgggaac agattgcctc
     7141 tctgcgcgga ctggggcaca gggttgaagt tttgtttgtc gaggcggaag aaagcgtgtt
     7201 ggtccgccgg ttttccgaaa ccaggcgagg ccaccctctg agtaatcagg atatgacctt
     7261 gttggaaagc ttaaagaaag aacgggaatg gctgttcccg cttaaagaaa tcgcctattg
     7321 tatcgacact tccaagatga atgcccaaca gctccgccat gcagtccggc agtggctgaa
     7381 ggtcgaacgt accgggctgc tggtgatttt ggagtccttc gggttcaaat acggtgtgcc
     7441 gaacaacgcg gattttatgt tcgatatgcg cagtctgccc aacccgtatt acgatcccaa
     7501 gttgaggcct tacaccggta tggacaagcc cgtttgggat tatttggacg gacagccgct
     7561 tgtgcaggaa atggttgacg acatcgaaag gtttgttacg cattggttac cgcgtttgga
     7621 ggatgaaagc aggagctacg ttaccgtcgc catcggttgc acgggaggac agcaccgttc
     7681 ggtctatatt gtcgaaaaac tcgcccgaag gttgaaaggg cgttatgaat tgctgatacg
     7741 gcacagacag gcgcaaaacc tgtcagaccg ctaattccgt caaaccatta tgccgtctga
     7801 aaccccgtcc tccgtttcag acggcatttc aaatattgaa aagaaagaac agacatggca
     7861 atccaatggt ttcccggcca tatgaacaag gcgaaaaaag ccatcgccga gcgtgcaaaa
     7921 agcgttgata tggtgattga aatgctggac gcgcgtatgc ccgcctccag cgaaaacccc
     7981 ctgcttgccc agctttccaa aggtaaaccc aaacttaaaa tcctcaacaa acaagatctt
     8041 gccgaccccg agcgcaccaa aatctggctc gaacactata acagccgccc cgacacctgc
     8101 gccatcgccc tcgattcctc cgaaacaggc gcacacggca aaattaccca agcctgtcgt
     8161 gccatgattc cccaccggca aggcatagac aaacccttgc gcgtcttgat ttgcggcatt
     8221 cccaacgtcg gcaaatccac cctcatcaac ggcatgatag gcaaaaaatc cgccaaaacc
     8281 ggcaacgaac ccggcatcac caaagccgaa caacgccttt tccttgccga cgacttctgg
     8341 ctgtacgaca cccccggaat gctgtggccg aaaatcattg tcgaagaaag cggctacaac
     8401 ctcgccgccg gcggttcagt cggacgcaac gcattggacg aagaagaagt cgccctcgaa
     8461 cttttagact acctccgccg ccactacctc cctatgctgc aagaacgcta ccaagccgac
     8521 aaagacccca gcagccactg ggacgaaaac gtttggctcg aatggatagc caaaaaacgc
     8581 ggcgcagtcc tcagcggcgg acggatcaac taccaaaaag ccgccgaaaa catcctcacc
     8641 gacttccgtg aaggcaaaat cggcagaatc accctcgaaa cgccgaacca atgggaaact
     8701 tggctcaaaa aagcccgtca gaaagaagcc gaactcaaag ccatacgcga agccagaaaa
     8761 gcagagcgta aagggcagaa gccttcggaa gcataaagaa tgccgtctga aaaatatttt
     8821 tcaggcagct tctctctact ccaaccgatt tcagacggca tatccaaacc catgccgttt
     8881 cagcacggat acccgtatga ccgacaaaat ttctcccgac gcgctgattg aagccgcact
     8941 gctgacccaa accgaaccgc tgaccgaaaa atctatgcgc gaattgtgtg tgccgccact
     9001 gtcgcaggac aaactgattg atgtgttggc gcagttgaaa acgcgttggc aggatagggc
     9061 gttgcaactg gtgcatacgc aagagggctg gcgttttcag attgttcaga cggcattcga
     9121 gcggctgggc agcctgcaag aacagcgtgc gccgcgctac tcccgcgccg tgatggaaac
     9181 actggcgatt atcgcctacc agcagcccgt aacgcgcggc gacatcgagg gcatacgcgg
     9241 cgtggcggtg tcgcagaacg tgatgcagac cttgcaggat cgggggtgga ttgaagtcat
     9301 cggacatcgg gacacattgg gaaaacccgc attgtgggca acaacggcaa cgttcctcag
     9361 cgatttgcgc ttggacggtt tggaagaact tcctccgctg accgaactgg gcgaactggt
     9421 tttgcccgat ttaatggaaa tgccgcctac ggatgaagaa gagccggaag ccgtaccgtc
     9481 cgataccctg cccaactgaa attccaaatg ccgtctgaaa cgcacattgc ttcagacggc
     9541 attgcaacaa ataagcagat aaaaacaagc attaagaaaa attaaggaaa aacttatttt
     9601 taatttaaaa aatcttagtt ataattcgta tatctaaagt tgatattgct tttgtcggta
     9661 gaattgctaa ggaatcctca cgatgcttct aacactttct ttgcgtgatt ttgtcattgt
     9721 tgaaaatctg aatctggatt ttcaaagcgg ctttaccgta ttgaccggag aaactggcgc
     9781 gggcaagtcc attactttgg atgcgattgg tctgctgttg ggcgataaag ccgattacag
     9841 ccaagtccgc agcggcgcaa aagaagcgca gttgtcggca ttgttcgata tttcccattt
     9901 gcctacttta aaagcagaat tgtatgaaca ggggctttta aacgacggag aagaagaact
     9961 cagtatccgc cgcattatcg atgccaaagg caaaagccgc agctttatca acaatcaggc
    10021 cgctaccttg gcgcaactca aagccgtcgg cagccagctt atcgacatcc acgggcaaaa
    10081 cgcccatcat tcgcttaatc aggaagccgc ccagcgcgaa ttgttggacg catttgcggg
    10141 cagcagggcg caggcggaaa ccgtcaggca gctttatcaa aactgggcaa actcaaaaaa
    10201 agcattgcag gaagcgcagg aacaggcaga aaacattgca ctcgaacaag agcgtctgga
    10261 atggcagttt aacgaattga atcagttgga cattaaacaa ggcgaatggg aagctctcag
    10321 ccaaagccac gacagccttg cccattctgc cgagctgttg caggctgccg aagaagtcgg
    10381 aagcaagatt gacggcgaca acggcatcca acgccatatc tatcaatgtc aaaaactatt
    10441 ggccaatctg caaaacatcg agccgcgctt tgccgagagc ctgaatatgt tggcaagcat
    10501 cgaagccgaa ttgggcgaaa tcagtgccaa tatgcgcgat gtggcaggac gcagcgacat
    10561 caaccccaat gaattggcgg cacaggaaca gcgtatgggc gaactgatgg ggatggcgcg
    10621 gaaataccgg atcgagcctg aagagttgcc tgccaagttg gcagaaatcg aagaacgcct
    10681 gcaaagcctg caagctgccg ccgatttgga cgcgctcgag cataatgttg cccacaattt
    10741 tgccgaatat caggaagctg cccacatcct ttctgccatg cgccatcagg cggcagagcg
    10801 tttgagcggc gaaacgaccg agcatatgca acaccttgcc atgaaaggtg cacgtttcga
    10861 catcgtcctg ttgccctcgt cgccgacggc acacggtttg gagcaggttc aatttcaagt
    10921 tgccgccaac aaaggcaatc cgccccgtcc gctgaataaa gttgcctccg gcggcgaatt
    10981 ggcgcgtatc agccttgcct tacaggttgt tgccagccaa tatacccaag ttcccaccct
    11041 gatttttgat gaggtcgata ccggtattgg agggggagtg gctgaaatgg tcggcaaggc
    11101 attacgttcg ttgggcagaa aacatcaggt gcttgccgtt acccatcttc cgcaagtcgc
    11161 atcctgcggc gaaaaccact ggcgggtgcg caagcacagc gagggagagc aaaccgtcag
    11221 cgaaatcagt atattggata aaatccaacg ggtcgaagag gttgcccgta tgttgggcgg
    11281 agaagtcatt accgatacga cgcggcaaca tgcggcagaa ttgctgcaac ttgcgtcgaa
    11341 aaatagttta ttttaaaatc aatcagttaa aaaataacta aaaataaaag tctaaaacaa
    11401 tagacagaac tcagataaat ccgtattatc acgctttctt aatcacttga acaagtgatt
    11461 gtgctgcacc cgtagctcag ttggatagag tatctggcta cgaaccagag ggtcgggcgt
    11521 tcgaatcgct ccgggtgcgc cagtaagaaa atacaatatg cgcccatcgt ctagcggtta
    11581 ggacatcgcc ctttcacggc ggtaaccggg gttcgattcc ccgtgggcgt gccaattcaa
    11641 aatgcctccg attatatcgg aggcatttct catttc
//
LOCUS       DAWHRS010000054        11553 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWHRS010000054 DAWHRS010000000
VERSION     DAWHRS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.054"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..807)
                     /gene="ilvE"
                     /locus_tag="WIB82_001030"
     CDS             complement(<1..807)
                     /gene="ilvE"
                     /locus_tag="WIB82_001030"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ3842339.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVAELKAA"
     gene            1117..1431
                     /locus_tag="WIB82_001031"
     CDS             1117..1431
                     /locus_tag="WIB82_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ3842340.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            1442..2611
                     /gene="lapB"
                     /locus_tag="WIB82_001032"
     CDS             1442..2611
                     /gene="lapB"
                     /locus_tag="WIB82_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ3842341.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            2575..3051
                     /gene="gloA"
                     /locus_tag="WIB82_001033"
     CDS             2575..3051
                     /gene="gloA"
                     /locus_tag="WIB82_001033"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ3842342.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(3224..5566)
                     /locus_tag="WIB82_001034"
     CDS             complement(3224..5566)
                     /locus_tag="WIB82_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ3842343.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSAQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     GGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAE
                     VETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQN
                     GILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTA
                     ENKPETVDTDFSDNLPSNNHIGTEETASAKPALPSGLAGFLKASSPETILEKTVAEVQ
                     TPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETVAETP
                     DFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENATEITLETPD
                     SNTSEADALPDFLKDGEEETVDWSIYLSKENIPNNADTGFPSESVGSDAPSEAKYDLA
                     EMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            5874..6404
                     /locus_tag="WIB82_001035"
     CDS             5874..6404
                     /locus_tag="WIB82_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ3842344.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            6407..6697
                     /locus_tag="WIB82_001036"
     CDS             6407..6697
                     /locus_tag="WIB82_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ3842345.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            6697..6975
                     /locus_tag="WIB82_001037"
     CDS             6697..6975
                     /locus_tag="WIB82_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ3842346.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            7024..7890
                     /locus_tag="WIB82_001038"
     CDS             7024..7890
                     /locus_tag="WIB82_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3842347.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            7961..8719
                     /locus_tag="WIB82_001039"
     CDS             7961..8719
                     /locus_tag="WIB82_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3842348.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSEDELHKFYEQQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            8792..9283
                     /locus_tag="WIB82_001040"
     CDS             8792..9283
                     /locus_tag="WIB82_001040"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ3842349.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            9314..10324
                     /gene="dusA"
                     /locus_tag="WIB82_001041"
     CDS             9314..10324
                     /gene="dusA"
                     /locus_tag="WIB82_001041"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ3842350.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANM
                     RE"
     gene            complement(10394..11281)
                     /gene="gluQRS"
                     /locus_tag="WIB82_001042"
     CDS             complement(10394..11281)
                     /gene="gluQRS"
                     /locus_tag="WIB82_001042"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ3842351.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGKWLIRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPALQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAVTAP"
     gene            complement(11298..11495)
                     /locus_tag="WIB82_001043"
     CDS             complement(11298..11495)
                     /locus_tag="WIB82_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842352.1"
                     /translation="MTKLVSRNASNERIADRAFDFFSPALMDGSATEEQYNALYDLTL
                     LEEPGMELNKDEIMALINSLK"
BASE COUNT         2588 a         2906 c         3337 g         2722 t
ORIGIN      
        1 cgcagctttc agttcggcaa ccgtgaaatt gcgttcgctg acggtatagc ccaaatcttt
       61 ggcaaccgtc agcacggaat cgcgggtaac gccgtgcaaa aactcgtcgg tcagcggttt
      121 ggtaatgatt tcatcgccgt taatcaggat aaagttggac gcgccggttt cctgcacgtc
      181 gccgttcggg cagaagagga cttggtttgc gccgtattcg gctttcgcct tcagcaccca
      241 gtgcatggcg gaagcgtagt tgccgccgca tttgacgcgg cccatatgcg gggcgcagcg
      301 gatgtgttcg gtttccacta aaattttgac gggcgagccg actttgaaat agtcgccgac
      361 gggggaagcc aaaatataca gcagggcggt ttcggaagga gaaccggcct tgccgataac
      421 gggatcggta ccgattaagg tcgggcgcag gtacagggcg gcaggcgcat cgggaatttc
      481 gtcggcggca cgtttgacca gttcgaccaa agcatcgaga taggcttggg tttcggggcg
      541 gggcaggtgc aaaatgtccg cactttgccg catacgcgcg atattggcag tcggacggaa
      601 cagtacgatt ttaccgtctg cctgacggaa ggctttcagt ccctcgaaac attcgctgcc
      661 gtagtgcagg gcgtgcgcgc ccggtgcgag ggagaggtct tgggaagatt gccattcggt
      721 cggctgccat ttgccttcgc ggtaggcgag gacgggcatt tgactgtgaa aaacgctgcc
      781 gaatacggcg ggtacgggtc tgctcatgat gtaaagcctt tcttattctg atatgtttca
      841 atgaacggtt tgaatttgaa gattgtaaag atacgcctgc aaacagggtt ttgacaagtg
      901 cgcggcgggt ttttctgtcg atgcggtgtc caatccgtta tttttcaaat ggaaaggaac
      961 ggtgtatttg gtaaaattgt cggcaatcgc atactccgta tgtcgtccga acacgctgcc
     1021 gcatcctatc cgaaaccgtg caaatcgttt aaactagcgc aatcttggtt cagagtgcga
     1081 agctgtccgg gcggcgtttt tatttacgga gcaaacatga aacttatcta taccgtcgtc
     1141 aaaatcatta tcctgctgtt cttcctgctg cttgccgtca ttaatacgga tgccgttacc
     1201 ttctcctacc tgccggggca aaaattcgat ttgccgctga ttgtcgtatt gttcggcgca
     1261 tttgtagtcg gtattatttt tggaatgttt gccttgttcg gacggttgtt gtcgttacgt
     1321 ggcgagaacg gcaggttgcg tgccgaagta aagaaaaatg cgcgtttgac ggggaaggag
     1381 ctgaccgcac caccggcgca aaatgcgccc gaatctacca aacagcctta agaaagccga
     1441 tatggacaac gaattgtgga ttatcctgct gccgattatc cttttgcccg tcttcttcgc
     1501 gatgggctgg tttgccgccc gcgtggatat gaaaaccgta ttgaagcagg caaaaagcat
     1561 cccttcggga ttttataaaa gcttggacgc tttggtcgac cgcaacagcg ggcgcgcggc
     1621 aagggagttg gcggaagtcg tcgacggccg gccgcaatcg tatgatttga acctcaccct
     1681 cggcaaactt taccgccagc gtggcgaaaa cgacaaagcc atcaatatgc accaaacatt
     1741 gcttgactct cccgatacaa ccggagccaa gcgcgcgcgc gtcctgtttg aattggcgca
     1801 aaactaccaa agtgcggggt tggtcgatcg tgccgaacag atttttttgg ggctgcaaga
     1861 cggtaaaatg gcgcgtgaag ccagacagca cctgctcaat atctaccaac aggacaggga
     1921 ttgggaaaaa gcggttgaaa ccgcccggct gctcagccat gacgatcaga cctatcagtt
     1981 tgaaatcgcc cagttttatt gcgaacttgc ccaagccgcg ctgttcaagt ccaatttcga
     2041 tgtcgcgcgt ttcaatgtcg gcaaggcact cgaagccaac aaaaaatgca cccgtgccaa
     2101 catgattttg ggcgacatcg aacaccgaca aggcaatttc cctgccgccg tcgaagccta
     2161 tgccgccatc gagcagcaaa accatgcata cttgagcatg gtcggcgaga agctttacga
     2221 agcctatgcc gcgcagggaa aacctgaaga aggcttgaac cgtctgacag gatatatgca
     2281 gacgtttccc gaacttgacc tgatcaatgt cgtgtacgag aaatccctgc tgcttaagtg
     2341 cgagaaagaa gccgcgcaaa ccgccgtcga gcttgtccgc cgcaagcccg acctcaacgg
     2401 cgtgtaccgc ctgcttggtt tgaaactcag cgatttggat ccggcttgga aagccgatgc
     2461 cgatatgatg cgttcggtta tcggacggca gctacagcgc agcgtgatgt accggtgccg
     2521 aaactgccac ttcaaatcac aagtcttttt ctggcattgt cctgcctgca acaaatggca
     2581 gacgtttacg ccaaacaaaa tcgaagttta accactaccg agaggaacac aaaaatgcgc
     2641 ttactccata ctatgctccg cgtgggcaat ctcgaaaaat ccctcgattt ctaccaaaac
     2701 gttttgggta tgaaactgct ccgccgaaaa gattatcccg aaggcagatt tacccttgcc
     2761 ttcgtcggtt acggcgacga aaccgacagc acggttttgg aactgacgca caactgggat
     2821 acggaacgat acgacttggg caacgcctac ggacacatcg cggttgaagt ggacgatgcc
     2881 tacgaagcct gtgaacgtgt gaagcggcag ggcggaaacg tcgtccgcga agccggcccg
     2941 atgaaacacg gcacaaccgt gatagccttc gtcgaagacc ccgacggata caaaatcgag
     3001 ttcattcaaa agaaaagcgg tgacgattcg gttgcctatc aaactgcctg ataccgccgc
     3061 cgccaatgcc gtctgaagcc tttaggggtt tcagacggca ttttgttgcc gtcgacctgc
     3121 tgtttgagcc tgtgccggtt caaactttat ccgttacacc gataaggcaa aaaaagatgc
     3181 cgtctgaaac ggcatccttg atctgcgaaa gggcagttgg gaatcaaata cccaattcct
     3241 gcgccaatgc ttgggcacgt ttgagtacgt cgccttccgc ttcttccagc aatttctgca
     3301 ctgtctcggc agcggcatcg cggtcgccga tttcgagata catttcggca aggtcgtatt
     3361 tcgcttcgga aggcgcgtca gaacctacag attccgaagg gaaaccggta tctgcattat
     3421 ttgggatatt ttctttcgag aggtagatgc tccaatctac cgtttcctcc tcgccgtctt
     3481 tcaggaagtc gggcaaagcg tctgcctcag aggtgttgga atcaggcgtt tccaaagtga
     3541 tttccgttgc attttcctca acggccggtg cttcagcagg ttgcaacagt gcggacaaat
     3601 catcggcaac ggtttccgct gcattttcct caacggcagg tacttcagaa ggttgaagta
     3661 atgcggacaa atcgtctgcg gtggcgttga agtcgggtgt ttcggcaacg gtttccgtta
     3721 tattttcctc aacggacggt gcttcggcag gttgaagcaa tgcggacaaa tcgtctgcgg
     3781 cggcgttgaa atcgggcgtt tcaggcgcag tttccgcgac ggcatcggtt tcgtacactt
     3841 tcaggaaatc gtgcaactct tccggtgttt ggacttcggc aactgttttt tccaagatgg
     3901 tttcgggcga ggaagccttc aggaagcctg ccagtccgga gggtaaggca ggttttgcgg
     3961 aagctgtttc ttctgtgccg atatggttgt ttgagggcag gttgtcggag aaatcggtat
     4021 cgacggtttc cggtttgttt tcggcagttt gggcgacaga ttccggttcg ggcgtgtcga
     4081 tgacgatttc gacagggttg tacgggttga aggtctcggg ctcgtacacg ctgtctgtgg
     4141 attcgatggc gttccaatcg gcatccgcgc gtttttgggt ttcttcatcc tgcgtaagtg
     4201 cgccggataa aatgccgttt tgcgcggctg ccaggctgtc gaaatccaag tcgatgcggt
     4261 tggaaggcgt atcggtttcg acatcgaacg tttgttttgc cgataactct tcttcagatt
     4321 ccccatctaa ggcaagtgtg tcgtttacat cgtttttcgg agcgggttcg ggcgttgccg
     4381 gagtttcgac ttcggcaaag gtgatttcta tgccgtcgtc tgccgcgtcg tcaaggtcag
     4441 gctcttcctc agggacggat tcttcggtac ggcgcgcgcg tttggattgg gcaaggcgca
     4501 aaagcagcag cagggcgatc aatgccgcgc ctccgccggc aagcagcaag gtgtacgaac
     4561 cgccgaacag tccgctttcg gtttcttctt cggcagaaac ctgttcgaca ggttcggaaa
     4621 cggcgttacc ggtttcgtcg gtcggcgtgt cgatggcaga agcggcggct tcttgggggg
     4681 cggattcggc agcggtttcc gatgcggcag tatttgcagc gggtacaggt tcgggtcgaa
     4741 cggccggttt ttccgctttt gcttcgggcg cggcaacttt tgcttcaggt ttttcaaccg
     4801 gtttctctac cgttgcctgt ttggacggtt cggacggcat ggatgcggtt tcggctttgg
     4861 gtttcgccgt ttgcggtttg ggttgttccg ctttgatcct gttcagattc ggaatgtgaa
     4921 gcacgctgcc cgcacgcagt ctgccgtgtg cggaaacatt tgggtttgcc ttcagcagcg
     4981 catcggcaac ctgttcgagc gtcaggtgtt tcgggcggat ggcggcggca atctgtttga
     5041 ccgtttcgcc tttgcggacg gtatgggttt tgccgttgta cgccggtttg acggctgcgt
     5101 tcgcgctgtc ttttttatcg gttttgcgga gggctttggc gttttgattt tcttgggact
     5161 ctgctgtcgg agcggttttg cggtgtgtct tgccgtctga aagtgcagat ttggttttgg
     5221 gcgagtagcc gacaggatcg aggatggcgg tgtattcgcg tacctgcgcg cctgcgccga
     5281 tgcggaacac caggacggga tcgcggactg cctgtgcgga agaaacggca atgacggctt
     5341 tgtcgcccaa cttgtggact ttggcggtca ggcctttttc ggaaacggta acgctgccgc
     5401 cgcctagcag ggctttggct tcttcgccgg ttacggtaat gctgccggaa aagggttcgt
     5461 caaggttgga ctggatattc agtccgccca gtcccgcatg tgcctgaaag gatgcggcaa
     5521 ctgcgacgga ggcggcaatc agtttgattt gtctgttgtt tttcaagatg tatcccctgt
     5581 gggttggcgg ctgaatacgg tttgaccgcg tacagtctgt aaatttcgtc atcatcgggc
     5641 atcggcgggg cagtcggccg gcgggcattt aatatgtgaa tgtaccgacc gccgccacat
     5701 tttaaacggc aatcattcgc cgtttttaca aattatgaca tatctccatc ttttttcaaa
     5761 aacatctgtg catatttgca tcaatcaaaa caaaatttgt tggttttgca ggtgcaaaaa
     5821 cagggttctg cctgtatgat tagcgtttat ttgatttgct ttctcatttg gatatgaaat
     5881 tcgtcagcga ccttttgtcc gtcatcctgt ttttcgccac ctataccgtt accaaaaaca
     5941 tgattgccgc aacggcggtc gcattggttg ccggtgtggt tcaggcggct tttctgtatt
     6001 ggaaatataa aaagctggat acgatgcagt gggtcggatt ggtgctgatt gtggtattcg
     6061 gcggcgcaac cattgttttg ggcgacagcc gcttcattat gtggaagccg agcgttttgt
     6121 tttggctggg cgcgctgttc ctgtggggca gccacctcgc cggtaaaaac ggcttgaagg
     6181 cgagtatcgg cagggagatt cagcttccgg atgccgtatg ggcgaaattg acgtatatgt
     6241 gggtcggttt cctgattttt atgggtatcg ccaactggtt tgtgtttacc cggttcgagt
     6301 cgcaatgggt caactataaa atgttcggct cgactgcact gatgcttgtt ttctttatta
     6361 ttcagggtat ttatctgagt acctgtctga aaaaggagga ttgactgtgg aatattttat
     6421 gttgctggca acagacgggg aggatgtgca cgaggcgcgt atggcggcac gtcccgaaca
     6481 cctcaaacgg ctggagacgc tgaagtcgga aggccggctg ttgacggcag gcccgaatcc
     6541 tttgccggag gactccaacc gcgtttcggg cagtttgatt gtggcgcagt tcgagtcttt
     6601 ggatgcggcg caggcttggg cggaagacga tccctatgtt catgcaggcg tgtacagcga
     6661 agtgctgatc aagccgttta aagcggtgtt caaataatgc cggccgtcga tttgatccgc
     6721 gaacgcctgc agacgctcga tccgctggtg ttggaaatcg gcgatgagag ccatctgcac
     6781 aaaggacacg cgggcaatac cggcggcgga cattatgccg ttttggtcgt tagcggccgt
     6841 tttgaaggcg taagccgcct gaaccgccag aaaacggtca aatcgctgct caaagatttg
     6901 ttttcaggcg gcatgattca cgcgctcggc atccgggcgg ctacccctga cgagtatttc
     6961 catacggcgg actgaatgaa gtctgcccga acatttcaat ttaaaattta aagagagaag
     7021 attatgaaag caaaaatcct gacttccgtt gcactgcttg cctgttccgg cagcctgttt
     7081 gcccaaacgc tggcaaccgt caacggtcag aaaatcgaca gttccgtcat cgatgcgcag
     7141 gttgccgcat tccgtgcgga aaacagccgt gccgaagaca cgccgcaact gcgccaatcc
     7201 ctgctggaaa acgaagtggt caataccgtg gtcgcacagg aagtgaaacg cctgaaactc
     7261 gaccggtcgg cagagtttaa aaatgcgctt gccaaattgc gtgccgaagc gaaaaagtcg
     7321 ggcgacgaca agaaaccgtc cttcaaaacc gtttggcagg cggtaaaata tggcttgaac
     7381 ggcgaggcat acgcattgca tatcgccaaa acccaaccgg tttccgagca ggaagtaaaa
     7441 gccgcatatg acaatatcag cggtttttac aaaggtacgc aggaagtcca gttgggcgaa
     7501 atcctgaccg acaaggaaga aaatgcaaaa aaagcggttg ccgacttgaa ggcgaaaaaa
     7561 ggtttcgatg ccgtcttgaa acaatattcc ctcaacgacc gtaccaaaca gaccggtgcg
     7621 ccggtcggat atgtgccgct gaaagatttg gaacagggtg ttccgccgct ttatcaggca
     7681 attaaggact tgaaaaaagg cgaatttacg gcaacgccgc tgaaaaacgg cgatttctac
     7741 ggcgtttatt atgtcaacga cagccgcgag gtaaaagtgc cttcttttga tgaaatgaaa
     7801 ggacagattg cgggcaacct tcaggcggaa cggattgacc gtgccgtcgg tgcactgttg
     7861 ggcaaggcaa acatcaaacc tgcaaaataa ttctgaaaac gggatatggc ggcaagacgt
     7921 tcagacaggc gttttgccgc cgcgcaggac agggaatacc atgaaacaga aaaaaaccgc
     7981 tgccgcagtt attgctgcaa tgttggcagg ttttgcggca gccaaagcac ccgaaatcga
     8041 cccggctttg gtggatacgc tggtggcgca gatcatgcag caggcagacc ggcatgcgga
     8101 gcagtcccaa aaaccggacg ggcaggcaat ccgaaacgat gccgtccgcc ggctacaaac
     8161 tttggaagtt ttgaaaaaca gggcattgaa ggaaggtttg gataaggata aggatgtcca
     8221 aaaccgcttt aaaatcgccg aagcgtcttt ttatgccgag gagtacgtcc gttttctgga
     8281 acgttcggaa acggtttccg aagacgagct gcacaagttt tacgaacagc aaatccgcat
     8341 gatcaaattg cagcaggtca gcttcgcaac cgaagaggag gcgcgtcagg cgcagcagct
     8401 cctgctcaaa gggctgtctt ttgaagggct gatgaagcgt tatccgaacg acgagcaggc
     8461 ttttgacggt ttcattatgg cgcagcagct tcccgagccg ctggcttcgc agtttgccgc
     8521 gatgaatcgg ggcgacgtta cccgcgatcc ggtcaaattg ggcgaacgct attatctgtt
     8581 caaactcagc gaggtcggga aaaaccccga cgcgcagcct ttcgagttgg tcagaaacca
     8641 gttggagcag ggtttgagac aggaaaaagc ccgcttgaaa atcgatgccc ttttggaaga
     8701 aaacggtgtc aaaccgtaat ggcatttcca ataccgatgc cgtctgaagc ctttcagacg
     8761 gcattgcacg ttcaggtaag gaggacggct tatgcgtgcg gtcatacaga aaacggtagg
     8821 tgcaaaggtg gatgtcgtgt ccgaagccgg cacggaaacc tgtggcaaaa tcgacggcgg
     8881 gtttgtcgtg ttactcggcg taacgcatag cgacacagaa aaagatgcac gctatatcgc
     8941 cgacaaaatc gcccatttgc gcgtgtttga agacgaagcg ggcaagctga acctgtcttt
     9001 gaaagatgtc ggcggcgcgg tgctgctggt gtcgcagttt acgctttatg ccgacgcggc
     9061 aagcgggcgg cggccttcgt tttcccaagc cgcacctgca gaacaggcgc agcagcttta
     9121 cctgcgaacg gcggaactgt tgcgcggaca cgggattcat gtcgaaacag ggcgtttccg
     9181 cacgcatatg caggtgtcgc tctgcaacga tgggccggta accatactgc tggactcttt
     9241 catgacgcgg atttccccaa aaatgaaggt tgttccggat tgaaattgaa tccgcaatga
     9301 taaaatatcg acaatgaacg acaatacaca cacccttccc ccgcgccacc tgtccgtcgc
     9361 ccccatgctc gactggacgg acaggcacta ccgttacctt gcccgccaga ttacccgaaa
     9421 tacttggctg tacagcgaaa tggtcaatgc cggtgcgatt gtttacggcg acaaagaccg
     9481 ctttttgatg ttcaacgaag gcgagcagcc cgtcgccctg caactgggcg gcagcgatcc
     9541 gtccgatttg gcgaaagccg ccaaagccgc cgaggcatac ggttacaacg aggtcaacct
     9601 caactgcggc tgccccagtc cgcgcgtgca gaaaggctcg ttcggcgcgt gtctgatgaa
     9661 cgaagtcggg ctggttgccg actgcctcaa cgccatgcag gatgcggtca agattcccgt
     9721 taccgtcaaa caccgcatcg gtgtggacag gcagaccgaa taccaaaccg ttgccgattt
     9781 cgtcggcacg ctgcgcgaca aaaccgcctg caaaaccttt atcgtccacg cccgcaacgc
     9841 ttggctggac ggtctttccc ccaaagaaaa ccgcgacgtt cccccgttga aatacgatta
     9901 cgtttaccgc ctcaagcagg agtttcccgg gctggaaatc atcatcaacg gcggcatcac
     9961 caccaacgaa gcaatcgccg gacacctgca acacgttgac ggcgtgatgg tcgggcgcga
    10021 ggcgtaccac aacccgatgg tgatgcgcga atgggacagg ctgttttacg gcgatacccg
    10081 cagcccgatt gaatacgccg atttggtgca gcgtctctac acatacagcc aagcccaaat
    10141 ccaagccgga cgcggcacaa tcttgcgtca catcgtccgc cacagccttg ggctgatgca
    10201 cggtctgaaa ggcgcgcgga cttggcggcg tatgctttcc gacgcaacgc tcttgaaaga
    10261 caacgacggc agcctgattc tcgaagcgtg gaaagaggtc gaacgggcaa atatgcgcga
    10321 atagggcggg gctgtatgtg tgaaatgccg tctgaaggct tcagacggca tttgtgcgtt
    10381 tgtcgggcgg tgtttagggg gcggtaacgg cgtgtttcgg cactttgtcc atatcccagt
    10441 gtgccaccgc ccagtcgagc agttcggcag ggcggtcggt ttccggtgct tcgggcagct
    10501 tgaggtaacg gaacacttgg cggaggagtt gttcgcggcg gtttaaatcc aatgcggggg
    10561 cgagcgtctg tttcgaccac ttctgccctt gtgcgttggt cagcagcggc aggtgggcat
    10621 attgcggtgt cggaacgccc aaacactgct gcaaatagat ttggcgcggc gtggaaacga
    10681 gcaggtcttg tccgcggacg atgtgggtaa cgccctgttc ggcatcgtcg gcaacgacgg
    10741 cgagctggta tgcccagtaa ccgtctgcac gaagcaggac gaaatcgccg atgtcgcggg
    10801 cgaggttttg ggcgtaaccg ccgacgatgc cgtctgaaaa accgataatg cggtcgggga
    10861 cgcggatgcg ccacgccggc tgtttgcctt gcagtgcagg gcgttggccg gggtggcggc
    10921 aacgtccgtt atagacgaac ccgtctgcgc cccgccttgc cccggcctgc cagtctttgc
    10981 ggctgcaatg gcagggatag accagtccgg cggttttcag gcggcatagg gtttcttcat
    11041 acagggcgta acggcggctc tgataggcga cttctccgtc ccactcgaat ccgaatgcct
    11101 caagcgtgtg caggatatgg cttgccgccc ccggcatttc gcgcggcgga tcgaggtctt
    11161 ccatgcggat cagccatttg ccgccgtgcg cgcgcgcatc ggcataggaa gcgacggcgg
    11221 tcagcagcga gccgatgtgg agcagcccgg tcgggctggg ggcaaaacgt cctgtgtaca
    11281 tatctggtac agccccttta tttaagacta ttaatcaaag ccattatctc atctttattc
    11341 agttccatcc cgggctcttc aagcaaggtt aaatcatata gggcattata ttgctcttcg
    11401 gtagctgaac catccataag agcaggcgag aaaaaatcaa aggctctatc tgcaattctc
    11461 tcattacttg catttctact aaccagtttc gtcaattctg tatattttga aaagtttatg
    11521 gaaaaataaa acagcgaaaa agttttggtt tcg
//
LOCUS       DAWHRS010000055        11315 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWHRS010000055 DAWHRS010000000
VERSION     DAWHRS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.055"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            97..813
                     /locus_tag="WIB82_001044"
     CDS             97..813
                     /locus_tag="WIB82_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ3842353.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="WIB82_001045"
     CDS             962..1864
                     /locus_tag="WIB82_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ3842354.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="WIB82_001046"
     CDS             1907..2680
                     /locus_tag="WIB82_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ3842355.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEVGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="WIB82_001047"
     CDS             2792..4480
                     /locus_tag="WIB82_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ3842356.1"
                     /translation="MLNKDQFADNHFIRTIIEEDLESGKHTAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMHRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="WIB82_001048"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="WIB82_001048"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ3842357.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWQLFHTLSGELSV
                     NGGLVTAQASAFEFLGAKNDDDHEWFKAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFIAPMLVTTRSADVRVFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELGGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            5841..6224
                     /locus_tag="WIB82_001049"
     CDS             5841..6224
                     /locus_tag="WIB82_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ3842358.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            6517..6990
                     /locus_tag="WIB82_001050"
     CDS             6517..6990
                     /locus_tag="WIB82_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ3842359.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            7116..8219
                     /gene="mnmA"
                     /locus_tag="WIB82_001051"
     CDS             7116..8219
                     /gene="mnmA"
                     /locus_tag="WIB82_001051"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ3842360.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            8290..9960
                     /locus_tag="WIB82_001052"
     CDS             8290..9960
                     /locus_tag="WIB82_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ3842361.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETVSFQTVLKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            10072..>11315
                     /locus_tag="WIB82_001053"
     CDS             10072..>11315
                     /locus_tag="WIB82_001053"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ3842362.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSENIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVA"
BASE COUNT         2798 a         3047 c         3019 g         2451 t
ORIGIN      
        1 atttgtgcaa tgctttaatt cctctggcac gatttgaaac acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgtcagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc cggcctgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgataa ggcgcgaaga agaggcttcc ggcaggggcg accgcgtcgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggaacgct tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca catatcatgc ggcaataaag gagggcagga
      961 gatggacagc ctgatgacat tgctttcggt attgataccg atgttcgccg gattttttat
     1021 ccgtgttccc aagccttacc tgcccgcttt ggacaaggtg ctgtcggttt tggtgtatgc
     1081 cgtgctgctg ctgatcggcg tatcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggcgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcattcgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaagcgcg ggcatgtatt gtctgatgct
     1381 gctggtgttc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcgggcgg
     1501 gctgctgttt gccgcatcgg cagacggcgt gtcgtggacg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtgatgacc gaggcttacg gcgcggtatg
     1621 gggcagtatc gcgcttttga acgatttggc acgagagctg ttcgcgctgg catttattcc
     1681 gctgctgatg aagcgttttc ccgatgcggc agtgggggtc ggcggcgcga ccagtatgga
     1741 tttcacattg cccgtgattc ggggtgcggg cggcttggaa gccgtaccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgctcc gtttctgatg gtggtgtttt ccgctttggg
     1861 ctgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgagttgga
     2041 ggaggtcggc agcctgaaac gccatcacgg cggcgcatct tcgggcggaa acttgccgga
     2101 gggcctgccc gccgaccgcc aaacccggtg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa cacatccccg acggttcgtc gctgtttgtc agtatcggca cgacgatgga
     2221 ggccgtggtg tcggagctgg tcaggcggcg cggcagcctg agggtgatta ccaacaatat
     2281 ccacgccgcc tcgattgctt cggcgcggac ggattatacg gtcatcatca cttccggagt
     2341 cgtccgccct ttggacggcg gtattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaatgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgct tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat tcgcggacaa
     2821 ccactttatc cgcaccatca tcgaagaaga cctcgaaagc ggcaaacata cagctgtcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggctt atatttatga cggtttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggtt
     3061 gggcttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttcc cgtttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcaccgacgc ggctacacgc ccgaaggcct
     3721 gcgtctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaactccgcc ccgcgcctga tggcggtgtt
     3841 gaacccgctc aaagtaaccc tgaccaactt tgaagccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aggaaatggg cgagcgcgaa gtacctattt cacaaaccat
     3961 ctatattgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tcccgaaggc cgcaaagtca aaggcgtaat tcactgggtg tctgccgaac acgcagccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggctggca actgtttcac
     4681 acattgagcg gggaattgtc tgtaaacggc ggtctggtaa cggcacaggc atcggcattt
     4741 gaatttttgg gtgcaaaaaa cgatgatgac catgaatggt ttaaagcggc ggacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccaccca attactgacg ctggcggaac agcagggcgc gaaagtcttt
     4921 aacagcggac aggcgatgcg tgatttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttatcgcgc ccatgctggt aacgacccgt tccgccgatg tccgcgtatt tttgaaagaa
     5041 cacggcgaca tcatcattaa accgctcgac ggcatgggcg gtatgggcat cttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcagct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg gcgcggcgtg gcgcaggaat tgggcggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgctggc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatc
     5461 atgaaacaaa aaggtttcga tgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgtcgt ctgaaaggct tttgcttcac aaccgtttgg tttgttcggg
     5581 caggcagggt tttccctcag tcggcagaga tacgcttaat gccgtacgga cacaccgccg
     5641 ccccgttttc agacggtata tatagtggat taacaaaaat caggacaagg tgacgaagcc
     5701 gcagacagta caaatagtac ggaaccgatt cactcggtgc ttcagcacct tagagaatcg
     5761 ttctctttga gctaaggcgg ggcaacgccg tacaggtttt tgttaatcca ctatattgag
     5821 aacattttga aaggataccg atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag
     5881 gcggcatcag gcgcgtcatt aacgcattcg gctattcgat agacggcatc gccgccgcct
     5941 accgttacga agcggcattc cgtcaggttt tgtggctgaa cgtactgctg gtgtgcgcgg
     6001 catttttttg ggtttccgaa accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt
     6061 cggtcattgt cgaactgttc aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg
     6121 aaaaacacga gctggccaaa cgcgccaaag acgcaggttc tgccgcacaa ttgttcgcga
     6181 tgctgatgtt ggcggcggtt tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga
     6241 tttgtaatcc tttaggatta gtatgtggta tttattattc acttaatcta catcaaattt
     6301 ccaggcagta tttgtatgta tagtggatta acaaaaatca ggacaaggcg acgaagccgc
     6361 agacagtaca aatagtacgg aaccgatcca cttggtgctt cagcacctta gagaatcgtt
     6421 ctctttgagc taaggcgagg caacgctgta ctggtttttg ttaatccgct ataagattaa
     6481 gcacattccc cgtctgatat tataaggagc agaaagatga aaaaattatt ggcggccgtg
     6541 atgatggcag gtttggcagg cgcggtttcc gccgccggag tccacgttga ggacggctgg
     6601 gcgcgcacca ccgtcgaagg tatgaaaata ggcggcgcgt tcatgaaaat ccacaacgac
     6661 gaagccaaac aagacttttt gctcggcgga agcagccccg ttgccgaccg cgtcgaagtg
     6721 catacccaca tcaacgacaa cggcgtgatg cggatgcgcg aagtcgaagg cggcgtgcct
     6781 ttggaagcga aatccgttac cgaactcaaa cccggcagct atcatgtgat gtttatgggt
     6841 ttgaaaaaac aattaaaaga gggcgataaa attcccgtta ccctgaaatt taaaaacgcc
     6901 aaagcgcaaa ccgtccaact ggaagtcaaa atcgcgccga tgccggcaat gaaccacggt
     6961 catcaccacg gcgaagcgca tcagcactaa tctgctgaaa atgtttgaaa tgccgtctga
     7021 aaaagcccgg gctttcagac ggcattttta tgcccgcctt taaaatgtgt taaaatccgc
     7081 ctttaaaaac cgccgtttcc aaccatattc tacgcatgaa tacgacagca aacccttcca
     7141 atatcatcgt cgggctctcc ggcggtgtcg attcttccgt aaccgccgcc ctgctcaagc
     7201 agcagggtta tcaagtgcgc ggtgtgttca tgcagaactg ggaagacgac gacaacgacg
     7261 aatattgcag catcaaacag gattcgttcg atgccatcgc cgttgccgat attatcggca
     7321 tcgatatcga catcgttaat ttcgccgcgc aatataaaga caaagtcttt gcttattttc
     7381 ttcaggaata cagtgcgggg cgcacgccga atccggatgt gttgtgcaac gccgaaatca
     7441 aattcaaatg ctttttggac tacgccgtag ggcagggcgc ggataccatc gccaccggcc
     7501 actatgcgcg caaagaagtt cgcaacggcg tgcattacct gctcaaaggt ttggatcgaa
     7561 acaaagacca aagctatttc ctctaccgcc tcaagccttt ccaactcgag cgcgcgattt
     7621 ttccgttggg cggtttggaa aaacccgaag tgcgccgcct tgccgccgaa tttaatttgc
     7681 cgactgccgc taaaaaagac agcacgggca tctgtttcat cggcgaacgc ccgtttcgcg
     7741 agtttctgca gaaatacctg ccgaccgaca atggtaaaat ggttacgccc gaagggaaaa
     7801 ccgtcggcga acacgtcggg ctgatgttct acacgctcgg acagcgcaaa ggtttgggca
     7861 ttggcggcgc gggagaaccg tggtttgttg cggctaaaga tttgacgaaa aacgaactga
     7921 ttgtcgtgca aggacacgac catccgctgc tttatacccg cagccttgtg atgaacgatt
     7981 tgagtttcac gctgcccgaa cgcccgaagg caggacgcta tacctgcaaa acgcgttacc
     8041 gtatggcgga cgcgccttgt gagttgcgct atttggatga tgaaaccgcc gagctggtgt
     8101 ttgacgaacc gcaatgggcg gttacgccgg gtcagtccgc cgtgctgtac gacggcgata
     8161 tctgtttggg cggcggtatc atccaaacga ccgacaaacc cgtcatcatc acacgataaa
     8221 ggtaatgccg tctgaaacgg ttttcagacg gcattgttcc gctcaattcc actttaaaga
     8281 ccgataccta tggaaaaaat ctggttagac agctacgaga agggcgtcag tgccgaaatc
     8341 gacatcacgc aatacaattc cgtcagcgac gtattccgcc aaagcgtgga aaaatttgcc
     8401 gaccaacctg cttttcaaaa catgggtaag acgctgactt atgccgaaac cggcaaactg
     8461 gcaacagatt ttgcttctta tctgcaaaac gtccttaaat tgcctcgagg agagcgcgtg
     8521 gcaataatgt tgcccaatgt actgcaatat ccggttgccc ttttcggcat tttgcaggca
     8581 ggtttggtag cggtgaacac caatccgctc tataccccgc gcgagctgga gcatcagttg
     8641 aaagacagcg gcgcgaccgc catcatcgtt ttggaaaatt tcgccaacac gctggagctg
     8701 gtgctgccgc gcacgcagat caaacacgtc atcgtcgcct ccgtcggcga aatgttcggg
     8761 ctgcttaaag gttcgctgat caatttcgtc atccggaaaa tcaagaaaat ggtgcccgaa
     8821 taccgtattc gggaaaccgt ttcctttcag acggtattga aagagggggc gaagcacgtt
     8881 ttccaacctg tcgcattaaa ccgtgaagat accgcgctgt tgcaatacac gggcggcacg
     8941 acaggtgttg ccaaaggcgc agtgctgagt cacggcaaca tctgcgccaa tatgcttcag
     9001 gcaaaagaat ggattaaaaa ccagttgcgc gaaggcaaag aaaccgttat cgccgccctg
     9061 ccgctgtatc atatttttgc cttaaccgtg aatctgatga tttttgccaa tgccggctcg
     9121 aaaatcatcc tgattaccaa cccgcgcgat atgaaaggct ttatcggaga actgaaaaaa
     9181 cagcgggtta atgtatttat cggcgtgaac acgctgttta acgcgatggt taaccgtcct
     9241 gattttgccg aagtcgattt ttcagaattg cggctgactt tgggcggcgg tatggcgacc
     9301 caaaaagccg ttgccgaaaa atggaaaaaa atcacaggca cgcccatcgt cgaagcctac
     9361 ggtctgaccg aagccagccc cggcgtgtgc tgcaacccct taaacatcga atcatacagc
     9421 ggcagcatcg gtttgcccgt ctcgtccacc gaagtcgaac tgcgcgacgc aaacggcaaa
     9481 gaagtccccg tcgggcagcc gggcgaattg tgggtaaaag gccctcaagt gatgcaaggc
     9541 tactggaacc gccccgaaga aaccgccaaa gccatagacg cgcgcggctt cttggaaacc
     9601 ggcgatattg ccgtcatgga cgaaaaaggc tggttgaagc tggtcgatcg taagaaagac
     9661 ctcgtcgttg tttccggatt caatgtttat ccgaacgaaa tcgaggaagt catcgcgcat
     9721 cacggcaaag tgatggaagt cgcctgtatc ggcgttcccg acgaaaaaac cggcgaggca
     9781 ctcaaagtat tcgtcgtcaa aaaagacccg tctttgacca aagaagaact tatcgccttt
     9841 tgccgcaccg aattaaccgc atataaagta ccgaaaaata tcgaattccg agacgagttg
     9901 cccaagtcca acgtcggcaa aatcctgcgc cgcgagttgc ggcaaagtac cgggaaataa
     9961 agaaaagatg ccgtctgaaa acagccgtcc accgttcaga cggcatccgt ccgtttgcca
    10021 gaaccgcgcg cttcacgtta aaatcacgca ttccaatacg ggtatttcat tatgaccaaa
    10081 ttcattttcg taaccggcgg cgttgtctcc tcactgggta aaggtatcgc cgccgcttct
    10141 attgccgcca tcctcgaatc gcgcggcttg aacgttacca tgctcaagct cgatccttat
    10201 atcaacgtcg atcccggcac gatgagcccg ttccaacacg gcgaagtgtt cgtaaccgac
    10261 gacggcgcgg aaaccgacct cgacttggga cactacgaac gctttatcga ttccacgatg
    10321 acccgccgca acagcttcag cacggggcag gtgtacgaaa acgtcatcgc caaagaacga
    10381 cggggcgact acctcggcgg cacggttcaa gtcatcccgc acattaccga cgaaatcaaa
    10441 cgccgcatcc acgaaggcgc ggcaggttac gatgtggcga ttgtcgaaat cggcggcacg
    10501 gtcggcgaca tcgaatcgct gccgtttttg gaagccatcc gccagatgcg aagccagttg
    10561 gggcgcaaca acaccttgtt tgcccacttg agctacgtcc cctacatcgc cgccgcaggc
    10621 gaaatcaaaa ccaagccgac ccagcacacc gttaaagaaa tgttgagcat cggtttgcaa
    10681 cccgacatcc tgatttgccg tatggacagg acaatgcctg cggacgaacg ccgcaaaatc
    10741 gccttgttct gcaacgtgga agaacgcgcg attgtcggca gctacgatgt ggacagcatc
    10801 tacgaatgcc ccgaaatgct gcacgaccaa ggcatcgaca acatcattac cgagcagttg
    10861 cagcttaatg tgcagcaggc ggatttgacc gcgtggaaaa aaatcgtcca cgccatccaa
    10921 aacccgaaac acaccgtcaa aatcgccatg gtcggcaaat acgtcgattt gaccgagtcc
    10981 tacaaatcgc tgatcgaagc cttgaaacac gcaggcattc acaccgaaac cgacgtgcag
    11041 attacctttg tcgacagcga aaacatcgag aaaaacaagg gcgacgtttc catgcttaaa
    11101 gacatggacg ccatcctcgt tcccggcggt ttcggttcgc gcggtgtgga aggcaaaatc
    11161 gccgccgtgc gctacgcccg cgaaaacaac gtgccatact tgggcatctg cctcggtatg
    11221 cagattgcgc tgatcgaata cgcccgcgac gtggcaggct tgaaaggcgc gaattccacc
    11281 gagttcgacc tcaaatgcgc cgcgccagtc gttgc
//
LOCUS       DAWHRS010000056        11262 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWHRS010000056 DAWHRS010000000
VERSION     DAWHRS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.056"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            52..1005
                     /locus_tag="WIB82_001054"
     CDS             52..1005
                     /locus_tag="WIB82_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ3842363.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1110..1433)
                     /locus_tag="WIB82_001055"
     CDS             complement(1110..1433)
                     /locus_tag="WIB82_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842364.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVEIDLFGSNSTMYVCSITPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1606..2967)
                     /locus_tag="WIB82_001056"
     CDS             complement(1606..2967)
                     /locus_tag="WIB82_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ3842365.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEVIEVTTFRGGAKVHQNARGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGMDDIAERKLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSQATAGPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3399..5099)
                     /gene="recJ"
                     /locus_tag="WIB82_001057"
     CDS             complement(3399..5099)
                     /gene="recJ"
                     /locus_tag="WIB82_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ3842366.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLAVYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVREDDLSQTFITDGSLKACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5168..5881)
                     /locus_tag="WIB82_001058"
     CDS             complement(5168..5881)
                     /locus_tag="WIB82_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842367.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTDDLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAAG
                     AVLVLPMAALIAAANSSDK"
     gene            complement(5928..6440)
                     /locus_tag="WIB82_001059"
     CDS             complement(5928..6440)
                     /locus_tag="WIB82_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ3842368.1"
                     /translation="MKMFEQEDEWISKTQMKKQMNDLQDLGMALTKLSNDTLKKIGLD
                     ADLYEAVTAYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLVRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            6587..7918
                     /gene="pmbA"
                     /locus_tag="WIB82_001060"
     CDS             6587..7918
                     /gene="pmbA"
                     /locus_tag="WIB82_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ3842369.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8215..8418)
                     /locus_tag="WIB82_001061"
     CDS             complement(8215..8418)
                     /locus_tag="WIB82_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ3842370.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8661..8963
                     /gene="clpS"
                     /locus_tag="WIB82_001062"
     CDS             8661..8963
                     /gene="clpS"
                     /locus_tag="WIB82_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ3842371.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8965..11241
                     /gene="clpA"
                     /locus_tag="WIB82_001063"
     CDS             8965..11241
                     /gene="clpA"
                     /locus_tag="WIB82_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ3842372.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTPALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKI"
BASE COUNT         2686 a         3279 c         2830 g         2467 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt cgaccgtgtc
      661 accaagctga ttgaaaaagg tctgattgag attgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacgga tacggaagcc aagctccgta
     1081 tccgtttctt tatccctcca accgatacat caatcgaatt ttctgcatcg gattgcctca
     1141 tcttcgcaaa acattgccga agtttcgcgg ttgcacgcct gacgcacttt ctgccgcgcc
     1201 acgccttcat tccggtcgct tgcctcaaac gtcttctgaa aaggcgtaat gctgcataca
     1261 tacatggtgg aatttgaacc gaaaaggtcg atttcgacgg tattgccgcc gctttccaat
     1321 acggcgacac ggtgttccaa ctgcgccaga cgtgcttcca aatcgctgat tctgtcggca
     1381 aatgcaggca tggcggcaca cgcggtcaaa atgcccaaaa tacatttttt catcaatttt
     1441 tccttgttgc ttgaaccctg cccacccgga catccttttt ccgagaggca gggtcagact
     1501 ttatttacgg ggatgaagcc ccttacaaat caatataata catggaacgg cactttatgc
     1561 ttcatcccat gtattgaaac ttcattgctc cttgtttgtt gaccgttatt cccaatccgt
     1621 tcccacaacc ttcggcttgg gtttgcgcct gcgacggcgg cgttttttcg cctgtccttc
     1681 gtttttttcg tgccgggcgg cagcctcgtt tttggtcatt tccgtccgct gttccgtcga
     1741 tgccgtctga aacgccgtcc accactcggc aagggcgcgg tccgcattgc cggtttcggc
     1801 gcgcaagagc aggaaatcat aggcggcacg gaaacgcgcc tgtgcaaaca gtttgtgcgg
     1861 acgtgcgcct ttgcggtttt caaactgcgg ctgaaacatc caaatttcgc gcatcgtggc
     1921 ggaaaagcgt tgcggcacgc cccaaccgcg ttcgacggtt tcacgcatcg tattgattgc
     1981 atcggacagg gcgggcgcgg gtttcaaacc ctgttgcaga ttgcttttcc aatggcgttc
     2041 caactcgggc cacatcagag ccgccagtac aaaaccgacc gaaaccgatt tgtcggcacg
     2101 cagccgctca tcggtatttt tcagggcaag caccgtcatt tttccggcga tgccgtctga
     2161 aacgcgcaag gcattgagca gcggatggat gtcgtccggt atgtcaaatc cgttcaaacg
     2221 tttcagacac tcgcgcgcgt gccctgaaaa cagcagtttc atgatttcgt caaacagcct
     2281 cgctaccggt tcgtgcttca gacggcatat tgattcggca ataggtccgg cggtcgcttg
     2341 cgacaattcg aatcccaatt tccccgacag gcggattgcc cttaaaatcc tgacagggtc
     2401 ttcctgatac cgttcggcgg cattaccgat cattaccagc ttccgttcgg caatatcgtc
     2461 cataccgttg tggaaatcca aaatttcttc tttttccgga tcgtaataca acgcgttgca
     2521 ggtaaagtcg cgcctcatgg catcttcttc gatactgccg taggtattgt ctttcataat
     2581 cctgccgcgt gcattctgat gtactttcgc accgccccga aaagtcgtta cctcaatcac
     2641 ttccgcaccg ttcatcacat gaacgatttg gaaacgcctg ccgatgatgc ggctgcggcg
     2701 gaagagtttg tgcacctgtt cgggcgtggc atcggttgcg acatcgaaat ccttgggttc
     2761 gatgccgagc agcaggtccc tgaccgcgcc gccgaccaca taagcctgaa accccgcccc
     2821 tttcaggcgg cgtatgacgt tttcggcggc aaagctcaac atttcggcac ggatgttgtg
     2881 tctttcggca ggaatgatcg ttttactttc cgctttttta ctgctccgac cggaaggcag
     2941 catcttattt aaccattttt tcagcataat attctaaccc gaagagcggc atctccacgg
     3001 gcaaccgtgt tcagacggca tcgggcgcgg tttgcaagat gccgtaacca taaacaaaag
     3061 gcgtagaaac tacgccgtcc gaatcacgcc gccctcgcgg cggcaacgcg tcattataac
     3121 agattgccct ccaaaaaaat cagaccctgc ttttgtggca gagtctgaaa tttgatgtgc
     3181 cttctccgat tgggaacatt cccgtcgtac aaacaggcaa atgtttcaac acacaggacg
     3241 acacataaag caccgtccta tgtgttgccc tgatttggaa ggggttacgc tccctcccaa
     3301 ataaggtctg attctaccgc cctaaagggt ggggtttcaa ccgaaaagga aatacgatga
     3361 aggaacagaa atcacattca aacggcgttc tgccgcctct acgccgcttc ccagtagtcg
     3421 atataaagct gcaattcgag attgttgcgc cattcgttgg caacggggcg gtaaaccgtg
     3481 cggatgtatt cggggatgtc ttcgctgcaa cgccaaaaca tggcttcaaa ttcgcagccg
     3541 tctttctgca gccacgcttt tttgtgttta ccctccgcgc ccaaaggctg ctggcggacg
     3601 acgtggaact cgtcggtaaa gcttggcggc gcgaagccct gcccccaaac gtgtcgggca
     3661 aggttttgag cctgttccag cgtgatgtcg caggctttca ggctgccgtc ggtgatgaag
     3721 gtttgcgaca aatcgtcttc gcgcaccatc tcgcgcacgg cttcttcaaa ggccgtctga
     3781 aacgcgggaa tattgtgttc aagtatgctc aagcccgccg ccattgcgtg tccgccgaat
     3841 ttcaaaatca aatcgggatg gcgtttggac accaagtcca aagcatcgcg caggtgcaaa
     3901 ttgggaatgg agcgtcccga accgcgcact tcgccgttgt ccgccggagc aaacacgatg
     3961 gtcggacgat aaaaacggtc tttgaggcgg ctggcaacga tgccgaccac gccttgatgg
     4021 aagtcgtcgc gatacaccgc caaagtcgtc tgacctgaag gcagggtttc aggaaaagca
     4081 ttcagcgcgt cttgcagcat agactgctcg atttcgcgac gctcgatatt gaggttgttt
     4141 aactgcgccg ccagttcctg cgcctcggta tcatctcgcg ccaacaggca ggcaataccg
     4201 accgacatat cgtccagccg tccggcggcg ttgatgcgcg ggcccaacgc aaaacccata
     4261 tcaaacggct gcgccttgcg ccaatcgcgc cgcgccactt caaacaaggc gcggataccg
     4321 gggcgcattt tgccggagcg catccgtttc aaaccttgcg acacgaggat gcggttgttg
     4381 tggtcgagag ggacgacatc ggcaaccgtg ccgagtgcga ccaaatccaa aagttcgccc
     4441 agattcggct ctttgatgcc gtctgaaaaa taattgcggc ggcgcaattc ggcacgcaac
     4501 gccatcaata cataaaaaat cacgcccacg cccgccaagc ttttgcttgg aaaaccgcag
     4561 cctttttggt tcggattgac gatgatgcag tcgggcacgg tctcggcagg cagatggtgg
     4621 tcggtaacga tgacatccaa acccaattcc tgcgccctcg ccacgcctgc gatgctggcg
     4681 ataccgttat caaccgtaat cagcaaatcc acaccttgct ctgccgcgat ttcagccagc
     4741 tcgggcgtta agccgtagcc gtgttcaaag cggttgggca cgaggaaatc cactttcgcg
     4801 cccatcgccg ccaaaccgtc caaaccgacg gcacacgccg tcgcgccgtc ggcatcatag
     4861 tcggcgacaa tcaggatttt ttcctgacgc tcaaccgcat ccgccaaacg gcgggcggcg
     4921 gcttcgcaat tcgtcagcga ttgataaggc aggagggaag cgagtttgtc gtccaattcg
     4981 gcaggacttt gcacaccgcg cgaagcacaa agccgggcga ttaaaggatc ggcaccggcg
     5041 gtgagcaaat gattaaaaac gtcggtattg acggagcggg tttggatttt gacggacata
     5101 acgtggaact ttacagtgcg ggtgtgcttg aaatgccgtc tgaaggtttt gcagacggca
     5161 ttgccgttca tttgtctgag gaattcgcgg ctgcaatcaa cgccgccata ggcaagacca
     5221 gtaccgcacc cgccgcatcc agtatcaacg tggtgggcgt atatgcaata ttttcaaaca
     5281 acttggattt gtcggtatgt tttttcgtaa ccgtgtaata aatatcggca ggcacacttt
     5341 gctcaaaatg ataatcggcg ttcagttttt gcggtgtggc gtagtatttg cctttggcgg
     5401 agacgcagcg cgtgtaaatg gtccgattgt caagttcgac cgcttcaaac tcaagctgtt
     5461 tcagcttggc gatgtcggca ggtctgtcgg tatcgtagcg caggcaaagg tcgtcggtac
     5521 tgaaattctg gctggcgggc gattcgagtt tgaccggcag ggcttggtag gcaaagcgcg
     5581 ggttgggctc aaccatttga aactgcttgt ccaacccggc cttcaaaatg cccgtcagct
     5641 tcgccgaatc ttcaggattg acgacgaacc agtatttccc gcccatcatc accaggctgc
     5701 ccttttccaa ttgggcattg tctttggcaa ccacaccgaa ggcgcggatt tggtctttgt
     5761 caacgtgttt ataggcggtc gtttggctga acgggtcgtt catgccccac atcattgccg
     5821 tacagccatt cagcatcaag gttgccgcca aaacggcggc ggtcgtttta cggaacaaca
     5881 ttttatcctc caatctaaat aacggcggat gtcgtctgaa aatatgccta aatttccgcg
     5941 tccccgtttt ccatcacggt tttcaattct tgaaacagcg cgcggaagtt tttcggcggt
     6001 ttgttttgct cctgctcttt tttggtgttg cggacgagcg tcctcagctt gcccgcgtcc
     6061 gcatgcggaa aatccgacat aaactgcgtc aacgcgccgt cgtctgccaa cagccgtacg
     6121 cgcgcctgtt ccacgcgttg caaaaaggcg ttgtgcgccg catcgtcgcc gcgcagcttg
     6181 gcaagaaacg cctcgatggg cgcgggatcg gtatcgcgca tcagcctgcc gataaattgt
     6241 gcctggcgtt tgagcgcgcc gttggatgtg atttttttat aggcggttac cgcctcgtac
     6301 aaatccgcat ccaaaccgat ttttttcagc gtatcgtttg agagcttggt caacgccata
     6361 cccaaatcct gcaaatcgtt catctgcttt ttcatttggg ttttgctgat ccattcgtct
     6421 tcttgttcaa acatcttgat tctcgaattt ttccaatagc gcattttaac agaatgccgc
     6481 cgcgttcaga cggcattttg caccgtccgc cctgcaaacg gcgcgctttt gcccctgccg
     6541 cccgaaacgg ttaaaatgcc gcaaagcatc atcaagaaag atttctatgc tgttcaacca
     6601 caccgcttcc gaactcctcg acctctgccg ccgcacgctc gacttggcaa aagcgacggg
     6661 cgcaaccgcc gccgaagccg atttcagcga atcattggga caaagcgtca gcgtgcggct
     6721 gggcgaaatc gaacaaatcg agttccagca ggacaagtcg ctggacatta ccgtttacgt
     6781 cggcaaacgc aaaggccgcg ccagtactgc cgacttctcc gaaaaagccc tgcaagacac
     6841 cgtcaaagcc gccatcgaca tcgcccgcca caccgccgaa gacggttgcg ccggacttgc
     6901 cgatgcctgt ctgatggcaa aacacatcgg cgaccccgac ctttaccacg aatgggattt
     6961 ggatacggaa gccgccgtcg gcttggcaaa acaatgcgaa caagccgccc tgaacgagga
     7021 tgagcgcatc gaaaactccg aaggcgcggc ggtgcaaacc ggccattacc aatacgttta
     7081 cggcaacacc cacggttttg ccgcacaccg gcaaggcacg caccacagca tttcctgcag
     7141 cgtcgttgcc gccgacgaaa acgggatgca gcgcgactac tggtacgatt ccgcctgccg
     7201 ccatccggat atggacagcc ccgaaaccat cggtcaaacc gccgcccgcc gcactttgcg
     7261 ccgactcggc agccgcagca tcccgacagg cagctacccc gtcctcttcg ataccaccgt
     7321 ttcgggcggt cttatcggac acctcgtcgg cgcactctcc ggcggcgcgc tctaccgcca
     7381 aagcagtttc ctgatcgaca gcatcggcaa aaaagtcctg cccgatttcc tcaacctgcg
     7441 cgaagaaccg cacatccccc gctctttccg cagcagctat tttgatgcgg aaggcgttgc
     7501 caccgcaccg cgcttcgtga ttcaaaacgg cattgtcgaa ggctatttcc tcagcagtta
     7561 cagcgcgcgc aaactcggta tgcagaccac gggcaacgcc ggcggcgcgc acaacctgta
     7621 tttgaaccat acgcacgaaa cgcaatccga cctgctgaaa gaaatgggga cgggattatt
     7681 ggttaccgaa ctgatgggac agggcgcgaa caccattacc ggtgactact cgcgcggcgc
     7741 ggcgggtttt tgggtggaaa acggcgtgat tgcctacccc gtccacgaga ttaccgtagc
     7801 cggacgcttg caggatatgt accgcgacat tgtcggcgtg gcggatgacg ctttgcggcg
     7861 ttcgtccaac aaaatcggct cgattctgat tgcggggatg acggttgccg gaagctgaac
     7921 gccctgccga tgccgtctga agacgaaggc agcacgtttt gccccgtgcc tgcccgtttg
     7981 ccgttttatg ccgcaccgcc ttttgccgac aacattgcac attttttcat cagcactgat
     8041 tttcccttat ccgtcaaaat actaagccac ttgtccacaa cagggtacac acaagttcag
     8101 gataaggcgt gcggggattg gggtttttcc gccgccggca aaacggcaga caaaaacggc
     8161 gggacattgc ctgtcccgcc gttttcaaat atacatttga ccgtacatca ggaattaagc
     8221 agcctgaatg ttggcggcct gtttgccttt agggccggtg gttacgtcga aagagacgcg
     8281 ttggccttct ttcagggttt tgaaaccttc catattgatt gctgagaagt gagcgaacaa
     8341 atcttcgccg ccttcatcag gcgtgatgaa accaaaacct ttagcgtcgt taaaccattt
     8401 tacgataccg gttgccatta gaaacttcct atactcaaaa attaacaaaa tcagcaaaac
     8461 aaggcatacg gcaaaccgag ccgtctggcg ttccttccag cttgatatac tcggcggatg
     8521 cccaatcctg cttaaccgtc tttttacatt tgttaagaca aaacgtcaag ctatgttttc
     8581 aaaaaagtgg gaaaataggc aaaagtaccg aaaatacaac aaaaatgccg tctgaacgcg
     8641 gctttcaaat gggacacact atgaccgcgc aacaccaatc cgacacgctc ttgcaccgtc
     8701 tgaacaccct gccgccgaaa cgttacggcg ttttcctatt gaacgacgat tacaccacga
     8761 tggaatttgt cgttgaaatc ctgaccgaaa tcttcatgct cggacaagaa caggcggtag
     8821 cggtaatgct cttggttcat cacgaaggca aaggcctgtg cggcacttac acgcgcgata
     8881 ttgcccaaac caaacagcaa caagtcatgc agcgggcaaa agccgaaggg catccgctgc
     8941 aatgtattgt cgaggagatt taatatgctt gcacccgaat tggaacagat tttgcagcag
     9001 ctttaccgcg aggcgcgtaa ggctcattat gaatttatca gcctcgagca tctgcttttg
     9061 gtactcatcg aagaagatgc ctccgtgccg aacgtcttaa aactctgcgg cgcggatttg
     9121 aaagtggtgt ccgaacagct cgccgccagc gttgccgaaa acacccccct gattcccgaa
     9181 caccttttag acacggtcga aacccggccc acgctcggct tccaacgcgt gatgcaacgg
     9241 gcgatggtgc atacccagtc tgccggaaaa gccgcagtcg aaccgttgga cgttttggtc
     9301 gcgctgatga gcgaaaccga cagccacacc gtctatttcc tcaagctgca atcggttacg
     9361 cgttttgaag ttttgcgctg tattgcccac ggctctcccg atgaagatga agacgatggc
     9421 aactattctt cagacggcat ggacgacgat aatgaaaacc gcaccaaacc gggcaaaaac
     9481 cctttatcgg cgtacaccgt caacctcaac gccgaagtca aagccggccg tatcgaccct
     9541 ttgattggtc gcaaacacga aatggaacgg ctggtgcaaa tcctatgccg ccgccgcaaa
     9601 aacaatccgc ttttggtcgg cgaagcgggc gtgggcaaaa ccgcgctggc ggaaggtttg
     9661 gcacatcaaa tcgtcaacgg cggcattcca gacgcgctca aagatgccga agtgtacgcg
     9721 ctggatatgg gctcgctgtt ggcgggcacg aaataccgcg gcgactttga agcgcgggtc
     9781 aaatccgtct tgaaacagct cgaaaaaatc ccgcacgcca ttttgtttat cgacgaaatc
     9841 cacaccatca tcggcgcagg cagcaccagc ggcggcacca tggacgcgtc caacctgctc
     9901 aaacccgcgc tggcaaaagg ttcgctgcgc tgcatcggcg cgaccactta cgacgaatac
     9961 cgcaccattt tcgacaaaga ccacgcctta agccgccgct tccaaaaaat cgacgtggtc
    10021 gaacccaccg tttccgaaac cgtacaaatc ctgcgcggct tgaagccgat gtttgaagcc
    10081 ttccaccaag tccgctatac gcaaggcgca ctcgaagccg ccgccgaact ctccgcacgc
    10141 tacatcaacg agcgtttcct gcccgacaaa gccatcgacg tgatggacga agcaggcgcg
    10201 gcgcaacgga ttctgcccaa atccaaacag aaaaaagtca tcggcaaagc acaaattgaa
    10261 accgtcatcg ccaaagttgc gcggattccc gaaaaaaccg tgtcgcacga cgacaaacaa
    10321 gtgctgcaat tcctcggtcg cgatttgaaa aacatggttt acggtcaaga aaacgccatc
    10381 gacgcgctgg ttgccgccgt caaaatgtcg cgttccggcc ttgccctgcc cgacaaaccg
    10441 ataggcagtt tcctcttctc cggtccgacc ggcgtgggca aaaccgaagc cgccaaacag
    10501 cttgcctact cgatgggcgt accgctgcaa cgctttgata tgtccgaata catggaacgc
    10561 catgccgtat cacgactcat cggcgcgcct ccgggctacg tcggctttga acaaggcggc
    10621 cttttgaccg aagccgtcaa caaacagccg cattgcgtat tgctcttgga cgaaatcgaa
    10681 aaagcccacc ccgacatttt caacgtcctc ctgcaagtca tggacgcagg caaactgacc
    10741 gacaacaacg gcaagagtgc cgatttccgc aacgtcatcc tgattatgac caccaatgca
    10801 ggtgcggaga gtctcagccg ccccagcctc ggctttaccg ccaagcgcga acgcggcgac
    10861 gaaatgcagg caatcaacaa gctcttcacg cccgagttcc gcaaccgctt ggacgcgatt
    10921 atcccgtttg cacccttgtc cgaacccatc atcgtcaaag tcgtggacaa attcctgctc
    10981 cagctcgaac accagctcct cgacaaaaaa gtcgaagccg aattcacgcc ggcattgcac
    11041 aaatatctgg cggaaaaagg ttttgacccg caaatgggcg cgcgcccgat gcaccgcctg
    11101 attcaggaaa aaatccgcaa accgctcgcc gacgaactgc tgttcggcaa actatccgac
    11161 ggcggcttcg tgcggataga ctgggatgcg gcaaaagaag aagccgtgtt gaaatttaag
    11221 aaaagtaagg tcaaaatata gtggattaac aaaaaccagt ac
//
LOCUS       DAWHRS010000057        11150 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWHRS010000057 DAWHRS010000000
VERSION     DAWHRS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.057"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(112..939)
                     /gene="fghA"
                     /locus_tag="WIB82_001064"
     CDS             complement(112..939)
                     /gene="fghA"
                     /locus_tag="WIB82_001064"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ3842373.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVAPDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(948..2057)
                     /locus_tag="WIB82_001065"
     CDS             complement(948..2057)
                     /locus_tag="WIB82_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ3842374.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            2205..2612
                     /locus_tag="WIB82_001066"
     CDS             2205..2612
                     /locus_tag="WIB82_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ3842375.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(2963..3277)
                     /locus_tag="WIB82_001067"
     CDS             complement(2963..3277)
                     /locus_tag="WIB82_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ3842376.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(3288..4973)
                     /gene="rpsA"
                     /locus_tag="WIB82_001068"
     CDS             complement(3288..4973)
                     /gene="rpsA"
                     /locus_tag="WIB82_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ3842377.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGNEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(5128..5784)
                     /locus_tag="WIB82_001069"
     CDS             complement(5128..5784)
                     /locus_tag="WIB82_001069"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ3842378.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSDSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            complement(6028..6836)
                     /locus_tag="WIB82_001070"
                     /pseudo
     CDS             complement(6028..6836)
                     /locus_tag="WIB82_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(7039..7254)
                     /locus_tag="WIB82_001071"
     CDS             complement(7039..7254)
                     /locus_tag="WIB82_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ3842379.1"
                     /translation="MFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPIA
                     KIWKLVGWMGCSARSASWLITWYSAAG"
     gene            complement(7209..7343)
                     /locus_tag="WIB82_001072"
                     /pseudo
     CDS             complement(7209..7343)
                     /locus_tag="WIB82_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            7663..7769
                     /locus_tag="WIB82_001073"
                     /pseudo
     CDS             7663..7769
                     /locus_tag="WIB82_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7971..8663)
                     /locus_tag="WIB82_001074"
     CDS             complement(7971..8663)
                     /locus_tag="WIB82_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ3842380.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHSLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(8731..10707)
                     /locus_tag="WIB82_001075"
     CDS             complement(8731..10707)
                     /locus_tag="WIB82_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ3842381.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWGELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMCIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            10886..>11150
                     /locus_tag="WIB82_001076"
     CDS             10886..>11150
                     /locus_tag="WIB82_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3842382.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQI"
BASE COUNT         2616 a         2545 c         2988 g         3001 t
ORIGIN      
        1 tccgtagagt cggctccttc cggctcagac ggcatacgtt tatgccggca cttcttaccg
       61 ccctcgtgcc ggctatccca aaactgcttt gaacactgaa cgctctttgg tttacttcaa
      121 aaacgcggcg tgataagcaa tatgctcgcc aataaaactg gcgatgaagt aatagctgtg
      181 atcgtagcct ttatggaaac gcacatcgac cggctggttt gccgcacggc aggtttcgat
      241 aaaatcttcg gtacgcaatt gtgtcggcaa aaactcatct tccaagcctt gatcgatgcg
      301 cataccttgc actttatagc cttgttgaat gagtgagtta gcatcatatt gctgccattt
      361 ttcacggtct ttccctaaat aagcagtaaa ggctttttct ccccacggca cgaggcttgg
      421 cgataaaata ggcgaaaagg cagaaacact ttgataacgt tcctgattcc gcagcgccaa
      481 taccaatgcg ccgtgtccgc ccattgaatg ccccataatg gaacgtttgc cgttggtagg
      541 aaagtgtttc tcaatcagac ggggcagctc gttcaaaatg taatcataca tttgataatt
      601 cgccgcccaa ggctgttcgg tcgcattcaa ataaaagcct gcactctgtc ctaaatcgta
      661 agcatcatcg ttcggcactt gctctccgcg agggctggta tcgggggcca ccacaattac
      721 ttgatgttct gccgcataac gctgaaagcc tgatttggta atgaaatttt gttccgtaca
      781 cgtcaagccg gaaagccaat aaatcacacc aagcggtcga ttttctggat tatttggcaa
      841 atagacggca aatttcattt cgcattgcag cgtttgggca tgatgcgccc aaacttgttg
      901 cgaaccgcca aaaatttgat gttgttcaat cagtttcatc gcatacctta gtagtgaata
      961 acggcgcgga tcgatttacc ttcgtgcatt aagtcaaagg ctttattgat ttgatcgagt
     1021 gtcattgtgt gggttacaaa cggttctaac tcgatatcgc ctttcattga atcttccacc
     1081 attttcggca gttcggagcg acctttcaca ccaccaaatg cagagccttt ccaaacacga
     1141 cctgttacca attggaacgg acgcgttgaa atttcttgtc ccgcacccgc tacgccgatg
     1201 ataatggatt gtccccaacc acgatgtgca ctttctaatg cctgacgcat tacgtttacg
     1261 ttgccgatac attcaaaggt atggtcaatg ccccatttat taatgtctaa caacacatct
     1321 ttgatcggtt tatcgtaatc gttcgggttc aaacaatccg ttgcaccaaa ctgttttgcc
     1381 aactcaaatt ttgatggatt ggtatcaatg gcgataatgc ggccggcttt ggcttgacgc
     1441 gcaccttgca ccaccgccaa accaatcgcg cctaagccaa acacggcaac agagtcgcct
     1501 tcttgcactt ttgccgtatt atgtaccgca ccaatacctg tggtaacgcc gcagccgagc
     1561 aaacatactt gttcatggtt ggcttcaggg ttgattttcg ccagtgaaac ttcggcaaca
     1621 acggagtatt cactgaaagt cgaacagccc atatagtgat aaatcggctg accttgataa
     1681 gaaaaacgcg tcgtgccgtc cggcattaag cctttacctt gtgtatcacg cactgagacg
     1741 cacaagttgg ttttacctga acaacaaaac tcacattcgc cacattcggc ggtgtaaagc
     1801 ggaatcacgt gatcacccgg ttttacgctt gacacacctt cgcccacagc aacgaccaca
     1861 cccgcacctt cgtgtccaag caccacaggg aatacgcctt caggatcgct tcctgataac
     1921 gtaaacgcat cagtatggca cacgccagtg tgggtattgc ggattaacac ctcgccttta
     1981 cgcggcattt ctacgtcgat ttccacaatt tgtaagggtt ggttaggggc gaatgccacc
     2041 gccgcacgag atttgatggt tgaatcggct tgtttcattt ccattgtctt tcctcttaat
     2101 tcattgacaa agttgccttg cctgagcaac atagcaacag tttacacttt agagttaact
     2161 ctaaagcaag tgttttttac tgtttttgtt tagaaggagg gaaaatgact tatactactg
     2221 ccaaagccgc cgaaaaaata ggcatctccg cccacaccct acgtttttac gacaaagaag
     2281 gtttgttgcc caatatcgga cgtgatgaat acggtaaccg ctgttttacc gataacgatt
     2341 tgcaatggtt gggcttattg caatgcttga aaaatacggg gatgagctta aaagacatca
     2401 aacgctttgc ggaatgtacc gtcattggcg acgataccat tgaagaacgc ctttccttgt
     2461 ttgaaaatca aatagaaaat gtgaagtgtc aaattgccga attaaaacgc tatttagatt
     2521 tgcttgaata caaattggcg ttttaccaaa aagcgaaagc attaggctcg gtaaaagctg
     2581 taaatttgcc gcaaattcct gaaacggctt agttttagtg caataagcag gatttttgtc
     2641 ctgttcgtaa tagaaatcaa aaatttgttg tttaaaaagc ggattgtaag caatgatata
     2701 gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtgtaaa tagtacggaa
     2761 ccgattcact tggtgcttca gcaccttaga gaattgttct ctttgagcta aggcgaggca
     2821 acgccgtact ggtttttgtt aatccactat aaaaaaatcc acgctctaat ccgttgcttg
     2881 cttggctcaa ggtattgaag caacggatga agttaaacca cagaaaaaac gccgcgtaac
     2941 agcggcgttt tgatgctaag gtttaattgg cattttcttt taaagccaag tcgacccgct
     3001 cgcgcaattc tttaccgggc ttgaagtggg gtacatgttt ttcaggtact tccacacgct
     3061 cgccggtttt gggattgcga ccgatgcggg caggacgatg gttcaaatcg aagctgccga
     3121 aaccgcggat ttcgatgcgt tgacctcggg caagcgatct agtcatggtg tcaaccaaga
     3181 cttttacgct gtactctacg tcttttgcca gaagatgcgt gccgtttttg gcggcaaaca
     3241 cttctgccaa acgaaccatt aactcagact ttgtcatgtc tgcaacctta ttcttgttcg
     3301 ccggagagtt tggctttcag caggtcgccc aagctggtgg tgccggcatt cgcattggcg
     3361 gcggcattga cggagttcag tgcttcgcgg ctttctttgg catctttggc tttaacggaa
     3421 agtttgatgc tgcggttttt gcggtcaacg gtaacgatga cggcttcaac ttcgttgcct
     3481 tctttcagtt tggtggtcaa atcttcaacg cggtcggctg caaattcgga agcaggcagg
     3541 tagccttcta cttcgtcaga cagggcgata acagcacctt tggcgtcaac agatttcacg
     3601 gaacctttaa ccaaagaacc tttgtcgttc acgctgatga agttgccgaa cggatcgcct
     3661 tccagttgtt tgatacccaa ggagatgcgt tctttttcca cgtcgattgc caatacgacg
     3721 gcttcgactt cttcgccttt tttgtatttg cgtacggctt cttcgccgga ttcggtccag
     3781 gacaggtcgg acaggtgaac caaaccgtcg atgccgccgg gcaggccgac gaatacgccg
     3841 aaatcggtaa tggatttaac cgcgccggag attttgtcgc ctttgttgtg gttggcggca
     3901 aattcttccc aaggattggc ttggcattgt ttcataccca aagagatacg gcggcgacct
     3961 tcgtcgattt ccaaaatcat gacttcgact tcgtcgccca gttgtacgac tttgctcggg
     4021 tgtacgtttt tgttggtcca gtccatttcg gagacgtgta ccaaaccttc gatgccttgt
     4081 tcgatttcga cgaatgcgcc gtagtcggtc aggttggata ctttgccgaa caggcgggtg
     4141 ccttgaggat aacggcgggt cagaccgctc caaggatctt cgcccagttg tttcataccc
     4201 aaggaaacgc gttgtttttc ttggtcgaat ttcaatactt tggcttcaac ttcctgaccg
     4261 acttccaaga cttcgctcgg gtgtttcacg cgccgccatg ccaaatcggt gatgtgcaac
     4321 agaccgtcga tgccgcccaa gtcaacgaat gcaccgtaat cggtaatgtt tttaacgatg
     4381 cctttgatga cggagccttc ttgcaggttt tccagcaggg ctttgcgttc ttcacccaaa
     4441 gtggcttcca gaacggcgcg gcgggaaaca acgacgttgt tgcgtttttt gtccagtttg
     4501 atcactttga attcgatctc tttgccttcg aagtgagaag tgtcttttac aggacgtacg
     4561 tcgaccaaag aacccggcag gaatgcgcgg atgctgctaa tcataacggt caggccgcct
     4621 ttgactttgc cgttgatgat gccggacagg atgttgccgt tttccatggc ttcttccagg
     4681 gcaatccaat cggcagcgcg tttggctttt tcgcgggaca gtttggtttc gccgaagccg
     4741 ttttcgacgg attcgatggt aacggtaacg aagtcgccga ctttaacttc aatttcgcct
     4801 tgagcgtttt tgaattcagc tacatcaatc agggattctg atttcagacc tgcgtttacg
     4861 gtaacgaagt tttggtcgat tgccactact tcagcggtaa tcacctcacc cgggttcatt
     4921 tcttgcaggg taaagctttc ttccaacagc tgagcaaaat tttccataga catatataac
     4981 tcttttcggt acaccgccaa ggggtgcggg gtgggttggt gggtgtctgc cgtccttggc
     5041 agggcagacg ggtaaataag gtttcagacg gcatatgggg aaattcatgc tgtctgaaac
     5101 gcaatgcggg cgattatacc tgaaaattta aactttacga taccaatcaa gcactttttt
     5161 tacagtttct tctatagtca ggcggctcgt atccaaaagc agggcatcgg gttgttgttt
     5221 caggggggca accttgcggt ttcggtctgc ctcgtctctg gcttcgatgt cggacaggat
     5281 gcgctcgaat gccaaacctt cgcaggggat gccgatttgt ttggcgcggc gttcggcacg
     5341 gattttggat tctgccgtca ggaagatttt aagttcggct tgggggaaga cgaccgatcc
     5401 ggtgtcccgt ccgtcggcga ccagtccttt ttcggtcaga aaatcgcgtt ggcgttgcag
     5461 cagggaggcg cggactttag gcaactgtgc gactgcggat gcgcccatgc cgatggcttc
     5521 tgtccggatg ccgtctgaaa cgtcttcgcc gccgagcagg atgcggctgt ctgaaaatac
     5581 ggcgggcagt ttttttgcca gttcggaaac gttttcttca tcgtgccatc ccacgccttg
     5641 tttttgtgca tatagggcgg tcaggcggta gagtgcgccg gtatcgagat aatcgtatcc
     5701 caatgcggcg gcaacgcggg cggcaaccgt gcctttgccc gatgcgcccg ggccgtcgat
     5761 ggcgatgact ttttgtctgt tcataagggg gattcctgat ggtttggggt atgggttttg
     5821 ccgtctgaag gatgtatttc ccattggggc ggattctacc tgttttaaag gacgattgtc
     5881 taagcagacg gaacgccgcc tgcccggaaa catccgacag aagtcggcaa gccggttcgg
     5941 atttcggcgt tgcgggtatt gtggcgggca tcaaggcaat gctgtctgaa agaggtatga
     6001 ccttcagacg gcatcgattg ctgcggatta gaacaggttg ctgacgaaaa tcagcagaat
     6061 caccagcggc acaagatatt tcacataagc aaaccaaata ttgaccgtcg tatggttgcc
     6121 tttataaagc aattcgtcct tcgcttcgtc cttcatcaca aaaccggcaa acagcgcgga
     6181 accgagcgcg gtcagcataa acaagatgtt gccgctgatg tagtcgaagg catcgaaaat
     6241 atttttgccg aacacggaac gtctttccac ggaccatagc tcagaatgga cgggatgttg
     6301 ccgaaaatga agatggcagc caatacaatc gtaatcgcgg cggtacggcg gattttggtt
     6361 ttttcctgaa tggtcgtaat caacacttca taaatggtca gcgaagttgt caacgcggca
     6421 atcaggagca gcgagaagaa aatcacggcg aacacagatc ccgcccacat atgtgagaac
     6481 acaatcggca agctttggaa caccaaagtc gggccggaat cgggggcaac gccgaagctg
     6541 aagagcgacg ggaaaatcat aaagcccgca agtatggcga tgatggtatt ggtaattgcc
     6601 gtgataactg ccgtctgaac cagattttcg tttttatcca aatagctgga caaggtaatc
     6661 atcacgccga aacccaagct cagggcaaaa aatacctgcc ccaaaacgaa gacgaacagt
     6721 tcggcggtaa tcttgctgaa atcaggtttc agatagaaag caaccccttc cattgcgccc
     6781 ggaagggtaa cgttgcggac gaccatcgcg attaggaaca aaaacagcag cggcatcagc
     6841 tattttgccg ctttttcaat gccgccgata acgcctttga ccaaaatcca ttggttcacg
     6901 gcgacaaaaa gcagcgtata aaacgcaatt tcccaagggc tgtttcaatg tgttcggcaa
     6961 agaagccttt tgtaaccaca ccgtcgacgg ggctggaaat attcaaattt cctccaataa
     7021 tattaacgat atagctgatt acccagccgc cgagtaccat gtaataagcc atgatgccga
     7081 acgcgccgag cagcccatcc agccgaccag tttccaaatt ttggcaatgg gtttgccgtt
     7141 catcgggccg ccgaacgcat ccagcgcgtt cacgcctttg cgccgtccga tgacattttc
     7201 caccaaaatc atcgggatgc cgataaccag catcgcgata cagaataaaa acacatacgc
     7261 gcaccaccgt tttcaccgac caaatacggg aaacgccacg tcgcgccgaa accgacagtc
     7321 gcgccggcaa cggtcaggat ataggttaat cggctggacc aggtttgacg attggtattt
     7381 gaaggggaag acatattgaa accgtgtccg attgagataa agcggaaatt ctacacgcgt
     7441 tttttaacag gaactgactg attgcttttc aactgtctag tgattttcca tgtaaaaggc
     7501 atatatttca gtgagtattt ctgatgagta taacccgatg aggaagaatc ggagtttata
     7561 aatagattaa tttgttattc ttctacatcg gtgtatggaa atgaaatttt gttaattata
     7621 ttaatgatag catttaaata atgatgaaag agagggaatt tgaaatatag tggattaaca
     7681 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa
     7741 cgctgtactg gtttaaattt aatccactat aaaaaaacca tctatacaag gggagaagta
     7801 tagatggcaa aacacattac ggggaaaacg tcttactcat aagcctgctt gaacaggcgt
     7861 tactcagaca aatggattat atcgtaaatt gattttttgc gttaaattgg gttaaaccat
     7921 atatttaatg agtatgtgga tgaatataaa tggaattctt ggatgttatt tcagaatttt
     7981 ggacattcga taaatttcca ttccccgggt ttgaggtttt ctgtttccag atgtgcgaat
     8041 cgccggcgat gaaggtgttg cacgcggttg ccggcggcgg cgatcatgcg tttgacttgg
     8101 tggtattttc cttcggtaat ggtcagcagc agggtggtcg ggtttttcaa aacggcatcg
     8161 gcggcacaaa cggtttcgtt ttcgtcgtgg agcagcacgc cgtttttcaa ggtttcgcag
     8221 agcgtttctc ctgtggggtg tttgagcgtt acttcgtaca gcttgggaat ttttctgctc
     8281 ggcgaagtca ggctgtggtt cagtttgccg tcgttggtaa tcagcaatac gccggtcgta
     8341 tctgcatcca gcctgccgac cgcctgcata tcgatgttcc gcatattgtc ggggaacagg
     8401 ctgaatacgc tgcggtagtg cttgggtttg tgcgaagttt cgtaatcttc aggcttgttg
     8461 agcatgatgt agaaataggg ttcgggaacg acggttactg cttccccgtc aatatccaac
     8521 gtttcgacgg atgaggaatc gatgtctgca tcggtgtcgt ccatgcaggt tccgttgatg
     8581 aaaacataac cgccggcaat cagccattgg cactgcttgc ggcttcctat gccttgatat
     8641 tgcaggtatt tgataagttt catgatggta tgggaaatgt gggatgaaaa agacaggact
     8701 gagaaagtcc tgtcatgtgc cgtatccgaa tcagcttcca atcagtttga ccctctgtcc
     8761 cggattgatg gtgttcaggt tgggattgag ccggcggatg tcgtcgatat ggatattgaa
     8821 gcgcgcggcg atacttttga aggtgtcgcc tttgcgcgcg gtgtaggata ctttttcaat
     8881 acgggtttgg gcaggggctg cctgcgccag gcggagtacc tgtccttttt ggatggtgtt
     8941 gcctttgatg ttgttggcga caatcaggtc ggctacactg acgttgtagc gtttggcaat
     9001 gttgaacagg gtgtcgcctt ctacaacgcg gtggatgctg gcatggagcg gggagatttc
     9061 ggcttcgccg tcggcagctt tggctacgcg cgtttccaaa cgtgcccggc gtttgccgtc
     9121 ggcggcaacg gtatgttgcg gagatgagga aatgttgtgt tcggcaactg tgtcaggcgt
     9181 gccgatgacg gcggagatgg tttcttcagc ctgtcggcgc aggttgtttc gggcaacaag
     9241 ctgcatgagt tcgtctgccg cctgcatgtc gttttggggg atgatctgcg cgagtgttgc
     9301 ggtttgggtt tcagacggca tggcggtctg tttttcggtt tgaggttgcg ctgcggctgt
     9361 cgcaagggca ggttcggcaa tacggacaag gggatcgggt tccgtacgga cggttttctg
     9421 cggcaaaggt gcgacggtaa tgtccgctgt ctgtgcggcg gcgggtcgga ttcgggcaat
     9481 gccgacgttc accgtgcctg ccggcatatt ggaacggtag gtgtcgggcg tattgtcgat
     9541 gtcgatggaa acgacggatt ccgatgccgt ctgaagggtt ttgccgttct tggcgacaag
     9601 gatgctgcgt cctgcgttga caaggttgcc gttcaggttg ttgaggcgtt tgatgtcggc
     9661 aatgcacatg ccggttgccg tcgagatgtc ggacaggctg gttttggcgg caggcgtata
     9721 gacttcccat gaaaacaggc tgtcgggtgc ggcgttgagg tagttgcttt ggaaggtttg
     9781 tacggacgcg acaggaagca gcagtttgcg tttgcttttg gggataaacg cggggacgtt
     9841 gaatgcgggg tttagggcga gcagctcgct ttgcgtgatg ccggcaagcc gggcgatggc
     9901 ttcgttgtcg agcggacgat ccggttcgac tgcctgaaaa tagggtttgt tgtctatgtc
     9961 gctgatattc atgccgaaag attggggagt ggcaataatg ttgcgcacgg cgagcagctt
    10021 ggggacatag ttgcgcgttt cgttgggcat acgcaggttt tcgtaggtcg gttcgagccc
    10081 ttgggcgcgg gcgcggttga tggcgcgtcc gacgttgcct tcaccccagt tgtaggcggc
    10141 aaaggcaagc ggccagtcgc cgaacagtcc atagaggtat tgcagatagt tgagtgcggc
    10201 atcggtggcg gcgtaaacgt cgtgcctgcc gtcgtaaacc ggtgtttttt ccaggccgta
    10261 atgcctgccg gtagcgggca taaactgcca taatcctgat gcgccgacgt gtgatttggc
    10321 tttggtgacg aacgcgcttt cgatgaaggg aagcagggcg gcttcggcgg gcatattgcg
    10381 ttttttgact tcgttggcga tatggtacat atagggtcta ctccggttga tgaccctgtt
    10441 gaaatagctg tggcttgcga tgaatttgct ttcgtggcgg cgtaccagtt cgggattgac
    10501 ttcgcccatc cggaagcctt ggcgcagctc gccccacagg ctgccggatt ggaaatattg
    10561 ttttgtcggg ggcaggtcga gtattgaaga gtttaagcgc ataatcgcca aaccgatttg
    10621 gtgtgatgag gtgttttggg cgtataggaa acccggacaa acggacagac ctgatgcggt
    10681 cagagcgatg gttttgagtt tggacatgat ttatattcgg cggaataaac atattttatc
    10741 gtccgatggt actgtttgac tgaaaaagcg tcaagtttta caggaacttt atgcggattc
    10801 ggaaatgggc ggccgatgtg ttttttgaca gaaatcctgt ttggaataga atatcggcag
    10861 gcaatctgtt tgaaacggac gcaatatgga ttcatggttt gaagatacgg cgatggggcg
    10921 gtatgttgca aaattggaac aggatttctt cgggcggtat ctggattcat accgtttttc
    10981 gggaatgtgt gcggttcagg tgggcggtcc gtggctgagc ctgtctgaag atgttgtctg
    11041 tgtgccgcga gatatgtcga tgtcggcgga gaatatggct ttggcggatg tttctgcgga
    11101 tatgctgctt tttccgcata cgcttgaagg cggtgttcct tcgcaaatcc
//
LOCUS       DAWHRS010000058        10986 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWHRS010000058 DAWHRS010000000
VERSION     DAWHRS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.058"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(1022..1792)
                     /gene="xth"
                     /locus_tag="WIB82_001077"
     CDS             complement(1022..1792)
                     /gene="xth"
                     /locus_tag="WIB82_001077"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ3842383.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFKNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1846..2133)
                     /locus_tag="WIB82_001078"
     CDS             complement(1846..2133)
                     /locus_tag="WIB82_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ3842384.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            complement(2539..3420)
                     /gene="nadC"
                     /locus_tag="WIB82_001079"
     CDS             complement(2539..3420)
                     /gene="nadC"
                     /locus_tag="WIB82_001079"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ3842385.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3495..4433)
                     /locus_tag="WIB82_001080"
     CDS             complement(3495..4433)
                     /locus_tag="WIB82_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842386.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGMLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVGRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            4635..5747
                     /gene="nadA"
                     /locus_tag="WIB82_001081"
     CDS             4635..5747
                     /gene="nadA"
                     /locus_tag="WIB82_001081"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ3842387.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYICRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKKKDVFNGMGPA"
     gene            5905..6234
                     /locus_tag="WIB82_001082"
     CDS             5905..6234
                     /locus_tag="WIB82_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEZ3842388.1"
                     /translation="MHWLFLTVAILSEVCGSSMLKLSGGFSKLWPSIGVVVSFSVCFW
                     ALSMTLKTMPLATAYAIWAGVGLVLTALVSVVFFGEKADFIGIVSIGLILLGVVLLNT
                     MSHMSGH"
     gene            6283..7791
                     /gene="nadB"
                     /locus_tag="WIB82_001083"
     CDS             6283..7791
                     /gene="nadB"
                     /locus_tag="WIB82_001083"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ3842389.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRAILSQGKPAIEWLLAQGVAFDR
                     NHNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRRPNIRVCERQMALDIQTES
                     GAACGLTVLDCRTQETHRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VGNLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLCAQA
                     IADGQAFQAEPFQRPSESLSAEAGIFSDDLQNTFSRPVLQTFNQRHLGILRNDTGLRR
                     AIAQLQLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(7969..8634)
                     /gene="pgmB"
                     /locus_tag="WIB82_001084"
     CDS             complement(7969..8634)
                     /gene="pgmB"
                     /locus_tag="WIB82_001084"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ3842390.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRYKNDNYVEMIQAVKPEDVYPGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTDFFDAIADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSGIAELTYAYLQNVWAQ
                     SGR"
     gene            complement(8647..10905)
                     /locus_tag="WIB82_001085"
     CDS             complement(8647..10905)
                     /locus_tag="WIB82_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ3842391.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRLFTVFGVRFDVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDGLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGGTPKTFEKRVIVTTSRDYQGLEAVKVAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRRGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTVALEK"
BASE COUNT         2409 a         2898 c         3067 g         2612 t
ORIGIN      
        1 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
       61 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      121 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      181 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      241 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      301 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      361 aaaaattcat caatatcggt actggatatt ttgtcatcac taataaacat cgctagagat
      421 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      481 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      541 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      601 tatagttatt gcctttggga gaatcaattc tgacagcaca taacataggt tcatctttac
      661 agtcgttata ttgggtttca tcaaactcaa ctgcatagca caataaaggc aataggaaaa
      721 aaaggagatt aatttattac tatacataag gcagcctgac tattcattct aaaataaatc
      781 cggtttttaa tatttgattc tgctccggat tatatacata ataaatttta ctgctccaaa
      841 atctgatatt aaccctatgg atttcataaa tttttcagaa aaataggctg tgcttgaaca
      901 atccgccgac tccggctgtt tgtcagggaa aaggcaatta ttttcggtta gtcagcagcc
      961 tatcctattg aatcacttaa agatatggat gtttgtcaac gccatatcaa aacacggtct
     1021 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1081 tctccaaatc aacgcggaca tccttcaacg ccgccgccat cgcaggcgac accaaaatat
     1141 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccaggtat
     1201 agaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1261 ttttgaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1321 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc cagtagcacc agtttgccgt
     1381 agcgggtcat ttcatcgcgg acaaattcgg ttaacgcggc aaaccattgt tctttatatt
     1441 tgaatttcgg gctgtcgagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1501 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcccga
     1561 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1621 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1681 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1741 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1801 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac accataccgc
     1861 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     1921 gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc agaccgcccg ccctcaggcg
     1981 gttcaaatgg ccggaaacgg cggtggccgg cagggataag gattttgcca gttcggcgat
     2041 attgcgttcg ctgtccaaca gttggatcag tatcgccata cgttcgggat gcgccataag
     2101 tttgagtatg gtgtggagcg gtattgtgtt catcggtatt cctaaaacct ttgccgcccg
     2161 ccaagcggat tgacgggcga cagatatgga tgattgtgat tatctttata aatcgtatta
     2221 taaatcaaat atttatggat gattgtacag gattgtatgt tccttttccc actttttttg
     2281 caaacacgcg taaaggcggg ctttcagacg gcataaaact gccgcccccg tcctttcccc
     2341 tgccctaccg atgcctccgc gctgtcgggc aaacaaaata cctgcctgaa acgctgtttt
     2401 ttctaaagca tcacgacctt cacgaaaaat gccgtctgaa gaatatttca gacggcattt
     2461 cctccttgag gcaataaccg gattacggtt aggcggttcc ttgtctgaca tatcagacaa
     2521 ctgccgacac cctaaaactc atgccaagaa atccaaacct atgtccaacg agcggctgct
     2581 gtgggtcaga tagccgaggg cgatgccgtc cacgccggtt tgcgccacgc gcttcaggcg
     2641 gtcgaagccg atgccgcccg atgcttcgca atagatggtg tgggggtggg cggtttgcgt
     2701 gtggcagcgg tttgccgctt ctttcagggt ttcgtcgtcc atgttatcca gcaaaatccg
     2761 ttccgcgccc gctgcgatgg cttcgtccag ttgtgccaac gtatccactt cgatttccac
     2821 gcaggtcaat gctccgacag cctgttttgc ctgctgcacg gcttgggcga tgctgccgca
     2881 ataggcgagg tggttgtctt tgatgagcac ggcgtcgtcc aaacccatgc ggtggttcac
     2941 accgccgcct gccctgacgg cgtatttttg caggacacgc agcaggggga tggttttgcg
     3001 gctgcacacg atgtctgtac cgtattcggc gacttcggca acggcacgcg cggtggcggt
     3061 ggcgatgccg cttaagtgcg tgaggtagtt gagcgcggtg cgttcggcgg cgagcagcgc
     3121 gcgggcgttg ccttcgacgg cggcaagcgt ctgacctgcg cggacggctt gcccgtctcg
     3181 gatttcggct tggaagcgga cggacggatc catcgtctga aaggcgagac gcgccaagtc
     3241 catgccggcg ataacgccgt cttcgcggct gacaaggaag agtttggcgg ttttgtcggg
     3301 ggcgatgacg gcggcggacg taatatcgcc gcgcctgccc aagtcttcgc tcaaggcttg
     3361 ttccaccatg gggcgcaaca gggtgtcggg cagggggaag agggtgtttt cagacggcat
     3421 ggggtttcct ttgttgaaaa agattgatgt tgcaccgatg ggcaaaactt cgtttgatgt
     3481 cgtctgaaaa cgggctaacg gctgcccagc ggcagtccga tgtccgaaat cagcctgtcg
     3541 ggcaggacgt cgtcgcggaa gcggcaaagc tgcgcgggac ggcctttgct gcccgataca
     3601 ccggtatccg acggctcgat gaggttttgc tgctgaatct ggcggcggaa gttttgcttg
     3661 tgcagcaatc tgccgctgat ggcttcgaca ctgttttgca gttgcagcag cgtgaattcg
     3721 ggcggcatca gttcaaaaat cacggggcgg tatttgattt tggcgcgcag gcgagacagg
     3781 gcggtcgcca gtacgcggcg gtggtcgtgg cgcatgggct gccccgtaag cgcgaagtcg
     3841 aagtttgcct gcggctcggc ggcctccgct atcaggccgc tttcatacag catttcatag
     3901 cgttgcaaaa cgtattcttc cgaccagttt tccggttcga ccccccaaca caaatgaatg
     3961 cgcttgagcc gcttttggcg cacttcctcc gtgtccgccg agtttgccca aatgcgcagg
     4021 cggccgacga cggcgtcgcg ctgcccgccg tcggtacgca aatcttccca cgggaaatag
     4081 ccgtagcagt cctgccattt cgcgtcggga tgcaggatgc tgtcggctgc ctcgcgcacc
     4141 agccccaaat agctgacgta caagacgggc atgccgtgtt cgttgcggcg gtgggtatcg
     4201 acaaaggtgt aaagctgttc cacatagccc ataggctgcg aagtctgctt ggcgacccac
     4261 agttttacgc ctgcctgcaa ggaattgcgc aggggggaga gcgggccgtt gggcaggagc
     4321 atgccttggg cgacggtcaa tacccgcagg ccgccgtcgg taacggcaat caatacggga
     4381 accagctcga cgatgctttg cggcggggct tcggcttcgg ggtaggcatc catgctcggc
     4441 tttctgcggg tgggaaaggc tttattgtaa accaaagcgg tttgtcggaa cgaagcgggt
     4501 cggttttacg gtattgcaaa cctgttttca gacgaccttg catattatac tcaagttgag
     4561 cataatgtaa aaacagtttg aaaaacagac ggcaaacctt catgccgtct gaaaaacagc
     4621 tacaaggaaa catcatgcaa accgccgccc gccgctcgtt cgactacgat atgccactca
     4681 tccaaacgcc gacttccgcc tgccaaatcc gtcaggcgtg ggcgaaggtt gccgacacgc
     4741 ccgaccgcga gacggcagat cgtctgaaag acgaaatcaa ggctttgctg aaggagaaaa
     4801 acgcggtctt ggtggcgcat tattacgttg atccgctgat tcaggatttg gctttggaga
     4861 cgggcggatg cgtgggcgat tcgctggaaa tggcgcgctt cggtgcggaa cacgaagccg
     4921 gtacgctggt ggtggctggt gtgcgcttca tgggcgagag cgcgaaaatc ctctgccctg
     4981 aaaaaacggt gctgatgcct gatttggagg cggaatgttc tttggatttg ggttgcccgg
     5041 aagaagcgtt ttcagcgttt tgcgaccaac accccgaccg cacggtggtg gtgtacgcca
     5101 acacttccgc cgccgtgaaa gcgcgtgccg attgggtggt aacgtcttcg gtggcgttag
     5161 aaatcgtatc gtatttgaaa tcacgcggcg agaagctgat ttggggaccc gaccgccacc
     5221 tcggcgacta catctgccgc gaaacgggcg cggatatgct gttgtggcag ggttcgtgca
     5281 tcgttcacaa cgaattcaaa gggcaagagc tggcggcgtt gaaggcggaa caccccgaag
     5341 cggtggtgct ggttcatcct gaatcgccgc aaagcgtcat cgaactgggc gacgtggtcg
     5401 gctcgaccag caaactgctc aaagccgccg tatcgcgtcc tgaaaaaaaa ttcatcgtgg
     5461 cgaccgattt gggcatcctg cacgaaatgc aaaagcaggc gcccgacaaa caatttatcg
     5521 ccgcgccgac ggcgggcaac ggtggaagct gtaaaagctg cgcgttctgc ccgtggatgg
     5581 cgatgaattc gctgggcggc atcaaatacg ccctgacaag cggacgtaac gaaatcctgt
     5641 tggacaggaa gctgggcgaa gccgccaaac tgcctttgca gcgtatgctc gactttgcgg
     5701 cagggctgaa gaaaaaagat gtgttcaacg gcatgggccc cgcctgattt gtcgttcgta
     5761 atgggtgcag cctgaaaata ccgcccgcct gctttcccaa gacgggattt gcacacaaaa
     5821 aatctttaac aacaagactg tttttttatt ttattgcatt gatataatta tactttcttt
     5881 catttatcag gtaaaaacat gcaaatgcac tggctctttc tgactgtagc aattttaagc
     5941 gaagtctgcg gttcttccat gctcaaactg agtggcgggt ttagcaaact gtggccttct
     6001 attggcgtgg tagtcagctt ttcggtgtgt ttttgggcct tgtctatgac actgaaaacc
     6061 atgccgctgg ctacagcata cgccatttgg gcaggcgtgg gactggtttt aacggcttta
     6121 gtcagcgtgg tgtttttcgg tgagaaagct gatttcattg ggattgtcag catcggtttg
     6181 attttgctgg gcgtggtgct tctcaatacc atgtcgcata tgtcggggca ttgattaatc
     6241 cactataccg tttcagacgg cctctcaaac aaggacaaca ccatgcaaac cgattgcgac
     6301 gtattgattg ccggaaacgg gctggcggca ctgacgctcg ccctgtcgct gcctgaatcg
     6361 ttccgcatcg ttattttgtg caaaaaccgg ctggacgaca ccgccagccg tcatgcgcaa
     6421 ggcgggattg cggcggcgtg gtcgggagag gacgacatcg aaaaacacgt tgccgatact
     6481 ttggaagcgg gcgcgggttt gtgtgatgaa gccgccgtcc gcgccatcct gtcgcagggc
     6541 aaaccggcaa tcgaatggct gctggcgcag ggcgtggcgt tcgaccggaa tcataacggc
     6601 ctgcacctga cgcgtgaagg cgggcatacc tgccgacgaa tcgcccacgt cgccgactac
     6661 acgggcgaag ccgtcatgca gagcctgatt gcccaaatac gccgccgccc gaacatccgc
     6721 gtttgcgagc ggcagatggc gttggacatt caaaccgaat caggcgcggc gtgcggactg
     6781 accgtcctcg actgccgaac acaagaaacc caccgcatcc gcgcccgcca taccgtactc
     6841 gcaggcggcg gcttgggaca gatttacgcc gccaccacca cgccgcccga atgcacgggc
     6901 gacgccatcg ccatggcgat acgcgcaggc tgcgcagttg gaaacctcga atttatccaa
     6961 ttccacccca caggcttggc aagaccgtct gaaaacggac gcaccttcct catttccgaa
     7021 gccgtgcgcg gcgaaggcgg catcctgacc aaccaagcgg gcgaacgatt tatgccgcat
     7081 tacgaccgcc gcgccgaact cgcaccgcgc gacatcgttg cccgcgccat cgcagccgaa
     7141 atcgccaaac aaacgcaaga cttcgtctcg ctcgacatca gccatcaacc cgcagcgttc
     7201 gtccgccggc atttcccatc catccatcga cactgtttat cccaatgcgg cctagacatc
     7261 acgcgccaag ccatccccgt ccgccccgtg caacactaca cctgcggcgg catccaaacc
     7321 gacccctgcg gcagaacctc cctgccgcag ctctacgcct tgggcgaaac cgcctgcact
     7381 gggctgcacg gagccaaccg ccttgccagc aactccctgc tcgaatgcgt cgtgaccgca
     7441 aggctctgcg cccaagccat cgcagacgga caagcattcc aagccgaacc attccaaagg
     7501 ccgtctgaaa gcctctccgc cgaagcaggc atcttttcag acgaccttca aaacacattc
     7561 agccgccccg tcctgcaaac gttcaaccaa cgccatctag gcatcctacg caacgatacc
     7621 ggcctgcgcc gcgccatcgc ccaactgcag ctttggaagc aaaaccaagc cgaaccgcac
     7681 accgcgtccg aatacgaaaa ccgcaacctg ctcgaatgca gcctcgccgt cgcccaagcc
     7741 gcatacaggc ggagacagaa catcggcgcg cattttaata gtgattgtta aggttggctg
     7801 gttagaaaaa aaatcgttca aataaatttg aatagcatgg ttaagagatt ggcatttagt
     7861 ttttagggtt gtgtaagttg ggttatcatt ctgttctaaa agcagaaagg ccgtctgaaa
     7921 cactgttcag acggcctttt acttccttga cactttatct gacgcgtttt acctgcccga
     7981 ctgtgcccac acgttttgca ggtaggcgta ggtcagctcg gcgatgccgg acaccagcgc
     8041 gatgtcgctg cccaagtcct cggctttgcc cacgccgatg ggcaaggcgc cggcggcttt
     8101 gatggcggct acacctgcgg cggcgtcttc aatgccgatg cagcggcgga tgtctgcgcc
     8161 cacgccctcg gcggcggcga ggaagatgtc gggggcgggt ttggaatgcg cgacggcggc
     8221 agggtcggca atggcgtcga agaagtcggt cagccccatg cgttcgagca ggaaggggcc
     8281 gtttttactg gcggacgcga gggcgatttt tttgccgttt gctttcaatg cttccagcag
     8341 cggcaaaatg ccggggtaca cgtcttcggg tttgactgcc tgaatcatct cgacgtagtt
     8401 gtcgtttttg tagcgggtca gttcggcaaa ctcggcttcg ccgacggttt tgccgccgtg
     8461 cgcgaggatg cgtttgagcg aatcgtcgcg cgacacgcct ttgagctgct cgttaaactt
     8521 gcggtctatg ccgatgccca gttcttcggc gagctttttc catgcgcggt aatggtattc
     8581 ggcggtatcg gtgatgacac cgtcgagatc gaagagcaca gcggtaaacg tcattttgcg
     8641 ccctccttat ttttccaacg caacggtgtg gctgccgttg agcgtgatgt ctttgccgta
     8701 cacctgcaaa tcaagcggtt cgcctttgag cagggtgaag acgacgtttt ctttgccgac
     8761 ggcgacttta atcagacggc ctcggtagtt gatatggaag gcgtagcctg tccatgcgcc
     8821 cggcaggaac ggtgcgaagc tgagtttgcc gccccaagtt ttcatttggg cgaaaccttg
     8881 gacgatggcg agccacgagc cggtcataga agtaatgtgc aggccgtctt cggtgtcgtt
     8941 gttgtagttg tccaagtcca ggcgggctgt gcgccggtac atttccacgg ctttttcttc
     9001 tttgcccagt tcggcggcga gaatagagtg gatacacggc gacaacgagc tttcgtgcac
     9061 ggtcatcggt tcgtagaagt cgaaattgcg gcgtttttcg tcgatattga aacggtcgcc
     9121 gaagaaatag atgccttgca atacgtccgc ctgtttgata aagggcgaac gcaggatttt
     9181 gtcccacgac catttctggt tgagcggcaa atcgtcgggc gaaagcgcgg acacggggcg
     9241 gatgtctttg tcgaggaagc cgtcgtgttg cacgaatacg ccgagttctt cgtcatgcgg
     9301 acggtacata ttcgcgctga tgtccgccca tttttccaac tcggcggcac gcacgttcaa
     9361 atccggacgc gggtatttcg ccaaggcttc gcgggtgtag tccaataccc aagcggcaag
     9421 ggtgttggtg taccagttgt tgttgatgtt gttttcgtat tcgttcgggc cggttacgcc
     9481 gtgaatcatg tatttacccc tgcgtttgga gaagtggacg cggtccgccc agaagcggga
     9541 cacttcgacc aagacttcca agccttcttt ggcaagatag ctttcgtcgc cggtgtagtt
     9601 ggtgtagttg tagatggcgt aaggaatcgc gccgttgcgg tggatttcct cgaaggtgat
     9661 ttcccattcg ttgtggcact cgatgcccgt aaacgttacc atcggataga gtgcgcccgc
     9721 caagccctgt tcgcgcgcgt tgtgctgcgc ctgcggcagt tggttgcggc ggtattgcag
     9781 caggttgcgg gtaacttcgg gttccgccag cgcgaggtag agcggcactg cgtaggcttc
     9841 ggtgtcccaa taggtcgcgc cgccgtattt ttcgccggta aagcctttcg ggccgatgtt
     9901 caggcgcgcg tcttcgccgt agtaggtgga gaacagttgg aacagattga aacggatgcc
     9961 ttgttgcgct tcgtcgctgc cttcgatgac cacgtcggcg atttcccaac ggtgcagcca
    10021 gcctgctttg tgcgcgtcca gcaaggtttc aaacgcaacg cctgcgactt tttccgacaa
    10081 ggcacgaccg gcgactttca ctgcttccaa gccctgataa tcgcggctgg tggtaacaat
    10141 cacgcgtttt tcaaaggttt tgggcgtgcc gccgacttcg gcctcgaaag aattggagac
    10201 ctgccagtcg gtttgtctgc cgccgagggc tttgaagcta ccggcgaaag tttgctcggc
    10261 gttgacgata aattgttcca caccaaaagg gttggcaacg gtttgggcgg caatgtagga
    10321 gaggccgtct gaaacgcctt tgtccaatac ctgccagaat ttttcttcgt agttggagtc
    10381 ttcgtttttc acgtcggcat cgatgatgga atcgatgcgg acttggtggg ttttgccgtc
    10441 aacggatacg gcttcccagc ggataaccgc cagctctttt tgcgcgacgg acaggaattt
    10501 gcacacatcg aaacgcacgc cgaatacggt gaacaagcgg cgcaacacgc cgtgctgcat
    10561 atcgagttcg acggagaagc cggcaacatc gtttttcgcc aagtccacct cttgaccatc
    10621 gacaaagatt ttgactttgc tgaaattgag cgcgttgatg gctttgccga aatatttggg
    10681 atagccgttt ttccaccagc cgacgcgggt tttgtcgggg aaccacacgc cggcgatgta
    10741 ggtgcccaag tggctgtcgg cggagtaggt ttcctcaaag ctgccgcgca tacccatata
    10801 gccgttgccc aaactggtca ggctctcttg cagccgtttg tgttcttttt ccagttttgc
    10861 cgaacgcagc gtccaagggc tgatttccat aattcttgtg tacatttatg aagctcctgt
    10921 ttggattgat ttgagggaat ggtgaaatct tatagtggat taacaaaaat caggacaagg
    10981 cgacga
//
LOCUS       DAWHRS010000059        10906 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWHRS010000059 DAWHRS010000000
VERSION     DAWHRS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.059"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(206..1423)
                     /locus_tag="WIB82_001086"
     CDS             complement(206..1423)
                     /locus_tag="WIB82_001086"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ3842392.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1623..2351)
                     /gene="rph"
                     /locus_tag="WIB82_001087"
     CDS             complement(1623..2351)
                     /gene="rph"
                     /locus_tag="WIB82_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ3842393.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2433..2897)
                     /locus_tag="WIB82_001088"
     CDS             complement(2433..2897)
                     /locus_tag="WIB82_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ3842394.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTAQELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3114..3890
                     /locus_tag="WIB82_001089"
     CDS             3114..3890
                     /locus_tag="WIB82_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ3842395.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4028..4555
                     /locus_tag="WIB82_001090"
     CDS             4028..4555
                     /locus_tag="WIB82_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842396.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKICILRTSFI
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRSS
                     TDYIVSRNLKFRHLY"
     gene            4909..5763
                     /locus_tag="WIB82_001091"
     CDS             4909..5763
                     /locus_tag="WIB82_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ3842397.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPKQGAAYVVVNFIALLELAKEGLVRIVQEDGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5804..7012
                     /gene="pncB"
                     /locus_tag="WIB82_001092"
     CDS             5804..7012
                     /gene="pncB"
                     /locus_tag="WIB82_001092"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ3842398.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEAELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            7104..8822
                     /gene="argS"
                     /locus_tag="WIB82_001093"
     CDS             7104..8822
                     /gene="argS"
                     /locus_tag="WIB82_001093"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ3842399.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGIAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERAAALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSNYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLSEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9392..10810
                     /locus_tag="WIB82_001094"
     CDS             9392..10810
                     /locus_tag="WIB82_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ3842400.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2613 a         3079 c         2688 g         2526 t
ORIGIN      
        1 tgaagcacca agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc gtcgccttgt
       61 cctgattttt gttaatccac tataaattta atccactata ttttttgttc caaagtcaaa
      121 atatgccgtc cgaacattcg ggcggcagac aaaacggcac tgcccgataa aggcagtgcc
      181 gttgtccgtt tcaaaccgtg aaacatcagc ccaaattaaa ggctttatgc aataccctgg
      241 ttgccagttc catgtatttt tcatcaatca atacggaaac tttgatttcg gaggtggaaa
      301 tcatttggat gttgataccc tcttcggcga gcgtgcggaa gattttggcg gctacaccga
      361 cgtgcgaacg catacccaaa ccgactgcgg agactttgca tacggtgtcg tcgccatcaa
      421 tagaagccgc gccgatactg tcttggcgtt ccgacaggat ttccaaagtc tgcttgtaat
      481 cgccgcgcgg tacggtaaag gaaaaatcgg ttgtgccttc gctgccgaca ttttggataa
      541 tcatatcgac ttcgatgttg gcatcggcaa ccgcgcctaa aatctgatag gcgacgccag
      601 gtttgtcggg tacgccgcgc acgttgatgc gggcttggtt tttatcgaat gcgataccgg
      661 ttacggcagc tctttccatg ttgtcgtcct cttcaaaggt aattaaggtg ccattgccac
      721 cgtcttgcag gctgctcagt acgcgcaggc gcactttgta ttttccggcg aattctactg
      781 aacggatttg caaaactttc gaaccgaggc ttgccagttc gatcatttct tcaaatgtaa
      841 ccgtatccat gcggcgcgct tcgggtacga cgcgggggtc ggttgtgtaa acgccgtcta
      901 cgtcggtata gatttggcac tcgtcggctt tgagcgcggc ggcaagcgcg acggcggaag
      961 tgtcggaacc gccgcgtccg agcgtggaaa tatcgccttc actgctgatg ccttggaagc
     1021 cggcaacgat gacgactttg ccggcggtaa ggtcggcacg cattttttcg tcatcaatgc
     1081 tttcgatgcg ggctttggtg tgggcggtat cggttttgag ggcgacctgc cagcctgtgt
     1141 agcttttggc atccacgccg atgtctttca atgccatcgc caaaaggccg atggttactt
     1201 gttcgccggt agctaagacg acgtccagct cgcgcggatc gggatgctct tgcatttcgt
     1261 gcgccagtgc gaccagtcgg ttggtttcgc cgctcatggc ggatacgacg actacgatgt
     1321 cgtgtccttc ggcgcgggct ttggcgacac gtttggctac gtttttgatg cgttcgggcg
     1381 agcctactga tgtgccgccg tatttatgta cgattaacgc catgtttcgt gctttcttgt
     1441 gggggttgtc gggcagcttg gtttgctgga aaaagggtta ttattactat tttttacatg
     1501 gaattcaaga acggactgcg ctttcccgcc tgccgtttga cagcggtcag cgaaaaacct
     1561 gttctttcag attgttgaca aaatgccgtc tgaacggttt tcagacggca tccggacgac
     1621 aatcaggcgg cggacaacgc attttgctgg tgttgcagca gttcgcctat gcctttttgc
     1681 gccagtgcaa ccagtttgcc caattcgtcc aaactgaacg gcgcgtcttc cgccgtcccc
     1741 tgtatttcga tgatttttcc cgatgcggtc atgacgatat tcacatcact gtcgcaaccg
     1801 gagtcttcgg gataatccaa atccaaaagc ggcacgccgt tcactacgcc tactgacaca
     1861 gcggcaacgg cttcgcggat ggggttttca ctcaaaatgc cgtctgaaac cagtttgccg
     1921 acggcgattt gcagcgcgac aaacgcaccg gtaatcgaag ccgtgcgcgt accgccgtct
     1981 gcctgaatca catcgcagtc aatcaagatt tgtcgttcac cgagtttttc catatccacg
     2041 accgcgcgca gggaacgccc gatcaaacgt tggatttctt gtgtgcgccc ggactgtttg
     2101 cccgccgaag cttcgcggcg catccgggaa gcagttgagg caggcagcat cccgtattcc
     2161 gccgttaccc agccttggtt tttaccgcgc agaaacggcg ggacgttttc atctatggaa
     2221 gcggtacaaa tcactttggt attgccgcat tcaataaggc acgaaccgtc cgtatgcggc
     2281 aggaaatgag gggtgatttt gatatcgcgc aggctgtcgg cggcgcgcga gatgcggatg
     2341 taatcaggca tactgccctc ccgttaaaaa cagataaatt aaaaagcctt aaatatgaaa
     2401 aatcacattt aaggccttca aactgaaaat ttctacgcct cttcggcttt gctgcggata
     2461 atcaaaagcg gcaggtggct ttggcgcatt accgtttcgg caaaactgcc cattaaaagg
     2521 tgcatcagcc cggtacgtcc gtgcgtaccc aacaccagca ggtcggcacc gttttcatcg
     2581 gcataatcaa ccaaatcctg cgccatttca cgcgcaccct tattggcaac cagcaggtgt
     2641 ttgacggtat tttccacacc cagttcctgg gcggtgcgct cggcggcatc caaaacttcg
     2701 ttgccttgcg cgacggcggc ggcttcgtag ctttcgtgtt gcaaaaattc gggggcgagt
     2761 gccatatatt cggcaggatt ggcaacgtgc accaaagtca ggcgcgcacc gttgaccccg
     2821 gcaagctcgg cggcatgttt cagggcattg atggacgttt cactgccgtc aacggcaaca
     2881 accaaatgtt tgtacatatc gtattctcct tttgcaccgc ctcgcggtgc cctcttgtcg
     2941 gatgggcgca gggacagttt gcgctgtttc attatagacc cgccgtcggg ctttatacaa
     3001 cagccgaaca gcccgaccgc tttccagtat aatatgccgc ttccgtgcag tcaggcattt
     3061 tttgccggct ttcgttcact ttttgatttg acgcaatctt gcaggattcg accatgtccg
     3121 acaacgcttt gacctcttcg cgacgcttcg gcggcatcgc cagactctac ggagactctg
     3181 ccttggcgca cttttcacag gcacacgtct gcgtagtcgg cgtgggcggt gtcggctcgt
     3241 gggcggtcga ggctttggcg cggacgggca tcggacgttt gactttgatt gatttggaca
     3301 acgttgccga atcgaatgtc aaccgccagc tgcacgccct gaccggcgac ttcggcaaag
     3361 caaaagttac cgccttgcgc gaacgcatta cacaaattaa tccgcaatgc gaagtgtttg
     3421 aaattgaaga tttcgttacc gaagacaatt tgccggaata cttcggaaaa ggttttgatt
     3481 tcgtcatcga cgcgatcgac caagtgcgcg tcaaagcagc aatggcggct tattttgtgg
     3541 aacgcaaaca accgtttgtc ctcagcggcg gcgcgggcgg acaaaaaaat ccggcgttaa
     3601 tccaaaccgc cgatttgagc cgcgtaaccc acgacccgct gcttgccaac ctgcgctaca
     3661 ccttgcggaa acgctacgga ttcagccgcg atacgaaagc aaatatgcgc gtgccttgcg
     3721 tgtattcgac cgaaaatatc gtgccgccgc agtctaggga ggcttgttcg gcagatgccg
     3781 ctccgcaagg cttgtcgtgc gccggctacg gtgcaagcat gctcgttacc gcttcgttcg
     3841 ggctatattg cgcacaggcg gcggtggaac acatcgcaga caaaaaataa gcaatgccgt
     3901 ctgaaacagg attcagacgg catttgaaca aactatggtt atgatttaag acaacaaagg
     3961 atacggataa aaaataacat aaaatatatg attcctaata atataccaag tatcggagag
     4021 ctatttaatg gaattcgtta ataatttagt tattttttca tttttattac taatgcttat
     4081 tccgatattt tttgtagtat atggtatata ccataagata cgttatcgca aaatatgtat
     4141 cctaagaaca agttttatat tattagtggt aatactttgc agtatgtatt acatatattg
     4201 ccgttatctt gaccaacaaa aagtagctta ttattgcata gatgaacaat gtatttctat
     4261 tgttcatcta tacaaagatt atggtataaa ctctcccaca tatgcgagaa tttacgcagg
     4321 aaaaatattg tttagatttc aagtaagagc taaaaattac gctgaattac ttatggaaga
     4381 tgatatatca attagtaaaa aaattttggg gaataaattt atcatttatg ggtcgctacc
     4441 tgtaatatac ggtaatgtag ataatattga agtaaaagaa gctactggtt atatagatag
     4501 atccagtact gattatattg tctcaagaaa cttaaaattc agacatttat attaattaag
     4561 aggttttagc aagagtgccg tcaaaatata gggcgcatca tcgaattcgc gaaagacaaa
     4621 cgctacgatg aacgtttcaa ggatttgaaa aaagaatcca taggctatct gaaccggcat
     4681 cccggtttgg tgtccgacta cctgaaggcg gcaatcaagc tgtcggttca gaaaaaccaa
     4741 catcagcacg cctaaaaccg tattcacaac ctgctccttt tcaaaacatt tgcatttaaa
     4801 agccgttata atgccgtctg aacatctgcc cgaccacatt atacgtgaat gtcggcagat
     4861 tgttttcttt tgtaaactta tattaaaatc cacttaccga ttcacgccat gccgcccatc
     4921 cctgccccat ctgcaccatc cgagcacact gtcgcatggg tattcggcca acccgttacc
     4981 gatttgcccc aggatttgtt tattccgccc gatgcattga aagtcgtatt gggcagcttc
     5041 caaggccctt tggatctact gctgtatctg atccgcaaac agaatatcga cgtactggat
     5101 attccgatgg tgaagattac cgagcagtat ctgcactaca tcgcccaaat agaaacctat
     5161 cagtttgatt tggcggcgga atatcttttg atggcagcaa tgctgattga aatcaaatcg
     5221 cgcctgctgc tgccgcgtac cgaaaccgtc gaagacgaag aagccgaccc gcgtgccgag
     5281 ttggtgcgcc gcctgctggc ttacgaacag atgaagctgg cggcgcaggg tttggacgcg
     5341 ctgccccgag ccggacggga tttcgcgtgg gcttacctgc cgctggaaat tgccgtcgaa
     5401 gccaagctgc ccgaagtcta tattaccgac ttgacgcaag cgtggctggg tattttgtct
     5461 cgggcaaaac acacgcgcag ccacgaagta atcaaagaaa ccatctccgt gcgcgcgcaa
     5521 atgacggcaa tcctgcgccg tttgaacgga cacggaatat gcaggtttca cgacctgttc
     5581 aatcccaaac agggcgcggc ttacgtggtc gtcaacttca tcgcactgtt ggagcttgcc
     5641 aaagaaggat tggtcagaat cgtgcaggaa gacggtttcg gagaaatccg aatcagcctc
     5701 aatcatgagg gggcgcattc agacggcatt tccggcacac gaggcgggcg cgatgtgttc
     5761 taatacgccc caagccgcca ccaaaaatcg ggagacacgc catatgaccg gcatcataca
     5821 ttcgctgctt gacaccgacc tctacaaatt cactatgctg caagtggttc tgcaccagtt
     5881 tccgcagacg cacagccttt acgaattccg ctgccgcaac gcctcgaccg tctatccgct
     5941 tgccgacatc agggaagact tggaagccga actcgacgcg ctctgccaac tacgcttcac
     6001 ccacgacgaa ctcgactacc tccgtagcct gcgtttcatt aaaagcgact ttgtcgatta
     6061 tctcgaactc ttccagctcc aacgccgctt tgtcgaaatc ggcacagacg ataaagaccg
     6121 tctgaacatc cgcatcgaag gtccgatgat acaggcgatg ttttttgaaa tcttcatcct
     6181 cgccattgtc aacgaacttt acttccgccg cctggaaacc cctgcagtca tagaagaagg
     6241 cgaacgccgg cttcaagcca aagccgcgcg cctcaaagaa atcgccgccg cacaaaaccc
     6301 cgacgaaccg cccttcctga tttccgactt cggcacgcgc cgccgctaca agctcgcgtg
     6361 gcaggaacac gtcatccgca ccctgctcga agccgcccca agcatcgtgc gcggcaccag
     6421 caatgtcttt ctcgccaaaa aactcggcat cacccccatc ggcaccatgg cgcacgagtt
     6481 cctccaagcc ttccaggccc tcgacgtacg cctgcggaat ttccaaaagg ccgcgctcga
     6541 aagctgggtg cacgaatacc ggggcgattt gggcgttgcc ctgaccgacg tggtcggtat
     6601 ggatgccttc ctgcgcgatt tcgacctcta tttcgccaaa cttttcgacg ggctgcgcca
     6661 cgacagcggc gacccttacg tttggggcga caaagcctac gcccactatc aaaagctcaa
     6721 aatcgacagc cgcaccaaaa tgctgacctt ctccgacggg ctggacatcg aacgctcttg
     6781 ggcattacac caatatttca aagaccgctt caaaaccggc ttcggcatcg gcaccaacct
     6841 gaccaacgat atggggcata cgcctttgaa tatcgtcttg aaactggtcg aatgcaacgg
     6901 gcagtccgtc gccaagctgt ccgactctcc gggcaaaacc atgaccaaca acagcacctt
     6961 cctcgcctac ctgcgccaag tgttcgacgt acccgaaccc gaaacgccgt aaaccggcag
     7021 aaaaagcgca caattcctgt ttctgccgca taaaatcttt taaaataccg cctgatttga
     7081 atttaaccga aagaccgaac ttcatgaacc tacatcaaac cgtcgaacac gaagccgccg
     7141 ccgcctttgc cgccgcaggc atcgccggca gccccgttgt tttgcagccg accaaaaatg
     7201 ccgaacacgg cgatttccaa atcaacggcg tgatgggtgc ggcgaaaaaa gccaaacaaa
     7261 acccgcgcga actggcgcaa aaggtcgccg acgcattggc aggcaacgcc gtcatcgaaa
     7321 gcgcggaagt cgccggtccc ggttttatca acctgcgcct gcgccccgaa tttctcgcgc
     7381 aaaacattca gacggccttg aacgacgcgc gtttcggcat agcgaaaacc gacaaaccgc
     7441 aaaccgtcgt catcgactat tcctcgccca atctggcgaa ggaaatgcat gtcggtcacc
     7501 tgcgttccag catcatcggc gacagcattt cgcgcgtgtt ggcatttatg ggcaacaccg
     7561 tcgtccgtca aaaccacgtc ggcgactggg gtacgcagtt cggcatgttg gtcgcttatt
     7621 tggtcgagca gcaaaaagac aatgccgcgt ttgagctggc ggatttggag cagttttacc
     7681 gcgccgccaa agtgcgcttt gacgaagatc ctgccttcgc cgacaccgcg cgcgaatacg
     7741 ttgtgaagct gcaaggcggc gatgaaaccg ttttggcatt gtggaaacag tttgtcgata
     7801 tttcgctctc gcacgcccaa gccgtttacg acacgctggg cttgaagctg cgccccgaag
     7861 acgtggcggg cgaatcgaag tacaacgacg atttgcagcc agtggtcgat gatttggttc
     7921 aaaaaggtct ggcggtcgaa gacgacggcg cgaaagtcgt attcttggac gagttcaaaa
     7981 acaaagaagg cgaacccgcc gcgtttatcg tgcaaaaaca aggcggcggc ttcctttatg
     8041 cttccaccga cttggcgtgt ttgcgctacc gtgtcggcac gcttcatgcc gaccgcctgc
     8101 tgtacgtcgt cgaccaccgc caagccctgc actttgagca actctttacc acttcccgca
     8161 aagcaggcta tctgccggaa aacgtcggcg cggcatttgt cggcttcggc acgatgatgg
     8221 gcaaagacgg caaaccgttc aaaacacgca gcggcgacac tgtaaaactg gtcgatctgc
     8281 tgaccgaagc cgtcgaacgc gccgccgctt tggtgaaaga gaaaaatccc gaattgggcg
     8341 cggacgaagc cgccaaaatc ggcaaaaccg tcggcatcgg cgcggtcaaa tacgccgact
     8401 tgagcaaaaa ccgcaccagc aattatgtgt tcgactggga cgctatgctt tcgtttgaag
     8461 gcaacaccgc cccctacctg caatatgcct acacccgcgt gcaaagcgtg ttccgcaaag
     8521 ccggtgaatg ggacgcaacc gcaccgactg ttttgagcga accgctggaa aaacagcttg
     8581 ccgccgagct gctgaaattc gaagacgtgc tgcaaagcgt ggcggacacg gcgtatccgc
     8641 actacctcgc cgcctacctc tatcaaattg cgaccctgtt cagccgcttc tacgaagcct
     8701 gtccgatact caaagccgaa agcgcaagcc gcaacagccg cctgcaactg gcgaagctga
     8761 ccggcgacac gctgaaacaa ggcttggatt tgctgggcat cgatgtgttg gacgtaatgt
     8821 aaaaccgcac cgcccgattg cggacaacag cctcgccatc cttatccgaa tctgaaaaaa
     8881 gcggcgcgat acaccgtatc cgccgcccct cccaaaatgc gaaacaaaca aacgccaagc
     8941 aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc
     9001 aaaaaattat aacccccttc ctgccgacgc acgcactttc cgcgcggcgc attccccttt
     9061 tcccgcccct caaatccgcc ttttcttcag gcagggtttc agcccgcctc ttttccctgt
     9121 tttcctttcc ccgacacgcg tgcgcgcccc ctgccgcact gtgctgcact ttcgcgcccg
     9181 gacggcatcg ttccgccatc cggttctctg ttttaaatac ccctgtttca gaaagaaatg
     9241 cagatgtttc aacacacagg acgacacata aagcaccgcc ctatgtgttg ccctgatttg
     9301 gaaggggtta cgcctcccaa ataaagtctg atcctgccgc cccgaaggac agatgtccga
     9361 gtggcgaagt ttcaaccgaa aaggaaatac gatgaatatt cacaccctgc tctccaaaca
     9421 atggacgctg ccgccattcc tgccgaaacg gctgctgctg tccctgctga tactgctggc
     9481 ccccaatgca atgttttggg ttttggcact gctgaccgcc accgcccgcc cgattgtcaa
     9541 tttggactat cttcccgccg cgctgctgat cgccctgcct tggcgtttcg tcaaaattgc
     9601 cggcgtattg gcgttttggc tggcggtttt gtttgacggg ctgatgatgg tgatccagct
     9661 cttccctttt atggacctca tcggcgccat caacctcgtc cccttcatcc tgaccgcccc
     9721 cgccccttat cagataatga ccgggctgtt gctgctgtat atgctggcga tgccgtttgt
     9781 gttgcagaaa gccgccgcca aaaccgactt ccggcacatt gccgtctgcg ccgccgttgt
     9841 ggcggcagcc ggctatttca ccggccattt gagttactac gaccggggtc ggatggccaa
     9901 tatcttcggc gcaaacaact tctactacgc caaaagtcag gcgatgctct acaccgtcag
     9961 ccagaatgcc gactttatta ccgccggcct ggtcgatccc gtcttcctcc ccttgggcaa
    10021 tcaacagcgt gccgccacgc atctgaacga gccgaaatct caaaaaatcc tctttatcgt
    10081 cgccgaatct tgggggctgc cggccaatcc cgaacttcaa aacgccactt ttgccaaact
    10141 gctggcgcaa aaagaccgtt tttcggtttg ggaaagcggc agttttccct tcatcggcgc
    10201 gacggtcgaa ggcgaaatgc gcgaactgtg tgcctacggc ggtttgcgcg ggttcgcact
    10261 gcgccgcgcg cccgacgaaa aatttgcccg ctgcctcccc aaccgtttga aacaagaagg
    10321 ttacgccacc tttgcgatgc acggcgcggg cagttcgctt tacgaccgct tcagctggta
    10381 tccgagggcg ggctttcaag aaatcaaaac cgccgaaaac ctgatcggta aaaaaacctg
    10441 cgccattttc ggcggcgtgt gcgacagcga gctgttcggc gaagtgtcgg catttttcaa
    10501 aaaacacgac aagggactgt tttactggat gacgctgacc agccacgccg actatcccga
    10561 atccgacatt ttcaaccaca ggctcaaatg caccgaatat ggcctgcccg ccgaaaccga
    10621 cctctgccgc aatttcagcc tgcacaccca attcttcgac caactggcgg atttgatcca
    10681 acgccccgaa atgaaaggca cggaagtcat catcgtcggc gaccatccgc cgcccgtcgg
    10741 caacctcaat gaaaccttcc gctacctcaa acaggggcac gtcgcctggc tgaacttcaa
    10801 aatcaaataa caacaatgcc gtctgaacgc accaacagcc ttcagacggc attttgcaga
    10861 cagaccgacc cttcaagccc acttttttca tcatctccga taaatt
//
LOCUS       DAWHRS010000060        10728 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWHRS010000060 DAWHRS010000000
VERSION     DAWHRS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.060"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(254..1513)
                     /gene="rho"
                     /locus_tag="WIB82_001095"
     CDS             complement(254..1513)
                     /gene="rho"
                     /locus_tag="WIB82_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ3842401.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            complement(1733..4117)
                     /gene="ppsA"
                     /locus_tag="WIB82_001096"
     CDS             complement(1733..4117)
                     /gene="ppsA"
                     /locus_tag="WIB82_001096"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ3842402.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGNRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4529..5350
                     /locus_tag="WIB82_001097"
     CDS             4529..5350
                     /locus_tag="WIB82_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ3842403.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5414..6076)
                     /locus_tag="WIB82_001098"
     CDS             complement(5414..6076)
                     /locus_tag="WIB82_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3842404.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTVLRRHNLPEKSIAEIRPY
                     AGHGAAGLLKLGAGITPDHPDYARWRTEYLDEYDRRYAQDTALFDGVDELIAELGRRG
                     IKWGIITNKPMRFTDKLVPKLGFAIPPAVVVSGDTFGEPKPSIKPMLYACRQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQPDYRIATPIELIGCLNKK
                     QV"
     gene            complement(6130..7089)
                     /locus_tag="WIB82_001099"
     CDS             complement(6130..7089)
                     /locus_tag="WIB82_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128146008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ3842405.1"
                     /translation="MSHHHEHHHHAHAHTHTANKKVLRVSFIIIAAFMLLEAFGGWLT
                     HSLALLSDAGHMFSDAFSLGVALWAFKLGEKETTLQKTFGYKRFEILTAMFNGLSLVV
                     IAVLIFYEAVKRLLYPPEIATLGMLVISVVGLLVNIGVAVYMLKNSDTEANVNMRGAY
                     LHVLSDLLGSIGAIAAAVLMMTFGWKWADPLASVFVAALVGRSGWSLLKQTLHILMEG
                     APENIHTDDLLAIIRNTDGVKSVHDLHVWTITSNINVLSCHIVVDGSMTVAESEQIAY
                     RIEHELSHKNIGHCTIQIESEHHPHKNEILCSPYAEAHHSHSH"
     gene            complement(7135..7962)
                     /locus_tag="WIB82_001100"
     CDS             complement(7135..7962)
                     /locus_tag="WIB82_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ3842406.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYSDEVGHVAIGNHWYQHLCRE
                     RGLEPIALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQSLKQGKKVV"
     gene            8392..9015
                     /gene="lolA"
                     /locus_tag="WIB82_001101"
     CDS             8392..9015
                     /gene="lolA"
                     /locus_tag="WIB82_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ3842407.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            9288..10427
                     /locus_tag="WIB82_001102"
     CDS             9288..10427
                     /locus_tag="WIB82_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3842408.1"
                     /translation="MKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            10561..>10728
                     /locus_tag="WIB82_001103"
     CDS             10561..>10728
                     /locus_tag="WIB82_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3842409.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKI"
BASE COUNT         2356 a         2726 c         3086 g         2560 t
ORIGIN      
        1 aatttataca aaaatgagcg cggttcggcg caaccttgaa tcaagttccc gcatcggttt
       61 tcattgccgg tacggatgcg ttcaagccgg ttttgcaaag gccgcgcttt cggcaagcgg
      121 acacggacac tgccgacggt tgcgccgtta acggggggag ggaggagctg cgccgaccgt
      181 gtgaatgaaa gtgccgtctg aaacccgatt ttcaggcttc agacggcatt tcgcattaat
      241 gcgggcggcg cgtttatttg ccgcgcatca gttcaaagaa atcgtcgttg tttttagagg
      301 ctttgatttt cccgtttaag aactcggtag cttcgatttc atccatcggg tgcaggaact
      361 tgcgtaagag ccacatacgt tgcaactggt cgttcggcac aagcagctct tcgcggcgcg
      421 tgccggattt gttgatgttg atggcgggga agaggcgttt ttccgccata cggcggtcga
      481 ggtgcaattc catattgccg gtgcctttga attcttcgta aatcacatcg tccatacggc
      541 tgccggtttc aaccaatgcg gtggcaatga tggtcagcga accgccttct tccacgttgc
      601 gcgccgcgcc gaagaaacgt ttggggcggt gcagcgcgtt ggcatcgaca ccgccggtca
      661 ggattttgcc cgaggtaggc acgacggtat tgtaggcgcg ggcaaggcgg gtaatcgaat
      721 ccagcaggat gaccacgtct tttttgtgtt ctaccatacg cttggctttt tcaagcacca
      781 tttcggcaac ttggacgtgg cgttgcgccg gttcgtcaaa ggtggaggaa accacttcgc
      841 cgcggacgga acggctcatt tcggttactt cttcgggacg ctcgtcaatc aagaggacga
      901 tgagttcgac ttcgggatag tttgaggtaa cggcgtgggc aatgttttgc agcatcacgg
      961 ttttaccgct tttgggcggg gcaaccaaga gggcgcgctg acctttgccg ataggggaaa
     1021 tcaggtcgat ggcacgtccg gtcaggtttt cttcggactt taagtcgcgt tccagcttca
     1081 actgttcggt cggaaacagc ggggtcaggt tttcaaacag gattttatgg cggcatactt
     1141 ccgggtggtc gccgttgatg gtatcaagcc tgaccagggc aaaatagcgt tcgttgtctt
     1201 ttgggacgcg cacgcttcct tcgatggtgt cgcccgtatg caggttgaag cggcggattt
     1261 gggtgggcga gacatagatg tcgtcggggc cggcaagata ggacgtgtcc gcgctgcgga
     1321 ggaagccgaa gccgtcgggc aggatttcaa gcgtgccgga gcaggtgaaa ccctcgcctt
     1381 ttttcatcat ctggcggacg atggcaaata cgaggtcttg tttgcggaat cggttggcgt
     1441 tttcgatgcc gtgttcttcc gccaattcta agagtttgga aatgtgcagg gtttgtaatt
     1501 cggagacgtg cataataatg atgtattttg aagaggaaaa agacaggcag atgccgtctg
     1561 aaagaagaag ctgaccgttg ccggttgctc ggggaagggg gaattgtagg cagtcggcgc
     1621 gtgggtgtca aatattatcg cggacggggc atcggcagaa aatgccgtct gagcggagct
     1681 gcttggaaaa aaataccccc gcgcttttca ggctcggggg tatgggcatt gattatttgt
     1741 tcaattcatt cgccaaatac agccaagttt cgatgacggt atccgggttc agagaaacgc
     1801 tttcaatgcc ttcctcaacc agccatttgg cgaagtccgg atggtcggac gggccttgac
     1861 cgcagatgcc gacatatttg ttctgcttgc ggcaggcgga gatggcaagg tgcagcatca
     1921 ctttgacggc agggttgcgt tcgtcaaacg attcggatac caagccgctg tcgcggtcga
     1981 gaccgagggt cagttgggtc atgtcgttcg agccgatgga gaagccgtcg aagtattgca
     2041 ggaattgttc cgccaatacc gcgttgctcg gcagctcgca catcataatc aggcgcaggc
     2101 cgtttttgcc gcgttccaag ccgttttctt tcagggcttt gacaacggct tcggcttcgc
     2161 ccaaagtgcg gacgaacgga atcatgattt caacgttggt caaccccatt tcatcgcgga
     2221 cgcgtttcaa ggctttgcat tccaaggcga aacagtcttt gaagttgtcg gcgacataac
     2281 gcgccgcacc acggaagccc aacatcgggt tttcttcatg cggttcgtat acgttgccgc
     2341 cgaccaggtt ggcgtattcg ttggatttga agtcggacat acggacgatg gttttacgcg
     2401 gataaaccga tgcggccaat gtcgccacgc cttcggcgat tttatcgacg tagaagtcga
     2461 caggggacgc gtaaccggcg atacggcggg taatttccgc ttttaattcg tcgtcttgtt
     2521 tgtcaaattc caacaaggct ttggggtgga taccgatttg gcggttgatg ataaattcca
     2581 tacgcgccaa gccgatgcct tcgctgggca ggttggcgaa gctgaatgcg agttcggggt
     2641 tgccgacgtt catcatgact ttgacgggtg ctttgggcat attgtccaag gcgacatcgg
     2701 taatctgtac gtccaacaga ccggcataga tgaagccggt atcgccttca gcacaggata
     2761 cggtaacttc ttgaccgttt ttcagcaatt cggttgcatt gccgcagccg acaacggcag
     2821 gaatgcccag ttcgcgcgcg ataatcgccg cgtggcaggt gcgtccgcca cggttggtca
     2881 cgatggcgga agcccgtttc atcacgggtt cccaatccgg atcggtcatg tcggtaacga
     2941 gtacgtcgcc ggcttcgacg gaatccatct cggaagcatc tttaatcagg cgcaccttgc
     3001 cctgaccgac tttctgaccg atggcgcggc cttcgcataa tacggttttg tcgccgttga
     3061 tggcgaagcg gcgcaggttg cggttgccct cttcttggga ttttacggtt tcgggacggg
     3121 cttgcaggat gtagagtttg ccgtccaagc cgtcgcgtcc ccattcgata tccatcgggc
     3181 ggccgtagtg tttttcgatg gtcagtgcgt aatgcgccaa ctcggtgatt tcttcgtcgg
     3241 taatggagaa gcggttgcgg tcttcttcgg ggacatcgac gttggttacc gatttgccgg
     3301 cttcggcttt gtcggtaaaa atcattttga tgtgtttcga acccatggtt ttacgcagga
     3361 tggcgggctt gcccgctttg agcgtgggtt tgaacacata aaattcgtcc gggttgaccg
     3421 caccttgtac gacgttttcg cccagaccgt aagaggaggt aacaaagacg acttgatcgt
     3481 agccggattc ggtgtcgagg gtgaacatca cacctgatgc gccgctgtcg gaacgcacca
     3541 tgcgttgaac gccggcggaa agggcgacga tgtcgtgttc gaagcctttg tggacgcggt
     3601 aggaaatggc gcggtcgttg tacagggagg cgaatacatg gtgcatcgct tctttaacgt
     3661 tttccaatcc gttgatgttc aagaaggttt cctgttggcc ggcaaatgat gcgtcgggca
     3721 ggtcttcggc ggttgcggaa gagcgtacgg caacggaaat gtccgcaccg ccggcatcgg
     3781 caaccatttt gttccatgcc gcttcgattt cggcatcgag ctgttcgggg aagggcgtgt
     3841 ccaaaatcca ttggcggatt tctttgccga cgcgtgccag ttcggcaacg tcttcgacat
     3901 ctaattttgc cagtgcggcg gaaatgcgtt cgctcagacc gttgtgtgcg aggaatgcgc
     3961 ggtaggcatc ggctgtggtg gcaaagccgc cggggacgcg aacgcctttt tcggtcagct
     4021 gactgatcat ttcgcccagc gaggcgtttt taccgcccac gcgttcaaca tctgtcatac
     4081 gcaggttttc aaaccagatt acgtagttgt cggccatttg tgtgtccaat ccaaaatatg
     4141 ttaaaaaaga aacaaatccg cgtgcttatt ttaagcgatt cgttcctctg ctgtcatgtg
     4201 ttttatccgt tttaaaatca tgatgccgtc tgaaaaattg cggtttcggc gtgtgtagcg
     4261 gtttgaaact tacagccggt atacttcttt ttttgggtat tttctttgta aaacaggtgg
     4321 tttgaatagg ttaatgtttt ttctgtttga tttttttgtt tattttttaa aattttctgc
     4381 caaaaaatac tttatataaa taattttatt tcaaaattat attgtgtctg tttgggtgta
     4441 atccgaggta ggtgtgctgc gagtgctttc cttgtgtctg ctgctgctgt tatgatggga
     4501 ttttaaacct gtgttttaag gatggaagat gagcagtccg cgccatgtgt tttacatttc
     4561 cgaccgtacc ggtctgactg ccgagaatat cggcgaggcg ttgctgaacc agtttggcaa
     4621 tctgtcgttc aaacgccata cgcatccgtt tgtcgatacg ccggaaaagg cgcgcgcggt
     4681 ggtggagaag gtcaatcgga gccggcagga aaacggtcag cgtccgattg cgtttgtcag
     4741 tgtggttgat gacgaaatcc gtcggattat caaaggggcg gatgcttttc agattaattt
     4801 ctttgagact tttttgggac tgttggagaa agaactcaat accgaagcca cggcatccgg
     4861 gcaggggcat cacagtatcg gtaatacgaa gcgttacgat gcgcgtatgg aggcggtgaa
     4921 tttctcattg aaccacgatg acggggtcag cgataagaac cttcaggaag cggatgtaat
     4981 cttgatgggt gtatcgcgtt cgggcaaaac gccgacctgc ctttacctcg ccctgcaata
     5041 cggcatccgt gcggcaaact atccgctgat tcccgacgat ttggaatcgg ccgatctgcc
     5101 gcgtatggtc aagccttata gggataagct gttcgggttg accatccagc cggaacgttt
     5161 gcaggccatc cgccaagagc gccgcccgaa ttcaacttat gccaaaatcg atacatgccg
     5221 cagcgaggtg gcggacgcgc agagtatgtt cagacggcat gggattccgt ttgcgaatac
     5281 gacggataaa tcggtggaag agcttgccgt acacatcctt caggcgtgca agttgaagcg
     5341 taggttctga tgtggtgtcg ggattgtacc ggagtgtggg aaatgccgtc tgaaaggttt
     5401 tcagacggca tttttatacc tgttttttgt taaggcagcc gattagttcg atcggtgtgg
     5461 cgatgcggta gtcgggttgc catgagccgg tatcgtcttc gggagcgatg tagccccatt
     5521 cggcgaggac ggttttcatg ccggcgttgc ggcctgcttg gatgtcgcgt tcggcatcac
     5581 cgacgtagag tgtgtgttgc gggtcggcgt ggatttgtct gcacgcatac agcatgggtt
     5641 tgatgctggg cttgggttcg ccgaaggtgt cgccgctgac gacgacggcg ggcgggatgg
     5701 cgaagccgag tttggggacg agtttgtcgg tgaagcgcat gggtttgttg gtgatgatgc
     5761 cccatttgat gccgcgcctg ccgagttcgg cgatgagttc gtccacgccg tcgaagaggg
     5821 cggtgtcttg ggcgtagcgg cggtcgtatt cgtcgaggta ttcggtgcgc caccgggcgt
     5881 agtcggggtg gtcgggcgtg atgcctgcgc cgagtttgag cagtcctgcc gcaccgtgtc
     5941 cggcatacgg gcggatttcc gcgatgcttt tttcgggcag gttatggcgg cgcaatacgg
     6001 tgttgagtgc gccgccgagg tctagggcgg tgtcggcgag cgtgccgtcg aggtcgaaca
     6061 atacggcttg tatcatgtgt tttcctttgg tggaggccgt ctgaaaggtt ttcagacggc
     6121 cttgttggtt taatggctgt ggctatgatg tgcttctgcg tagggcgaac acaaaatctc
     6181 gtttttgtgc gggtggtgtt cgctttcgat ttggatggtg cagtgtccga tgtttttgtg
     6241 ggacaactcg tgttcgatgc ggtaggcgat ttgctcggat tcggcaacgg tcatgctgcc
     6301 gtctacgacg atgtggcagg ataatacgtt gatattactg gtaatcgtcc agacgtgtaa
     6361 gtcgtggacg gatttgacgc cgtcggtgtt gcggatgatg gcgagcaggt cgtcggtgtg
     6421 gatgttttcg ggcgcgcctt ccatgaggat gtgcagcgtt tgcttgagca ggctccagcc
     6481 gctgcgtccg accaacgcgg ccacgaacac gctggcgagc ggatctgccc atttccagcc
     6541 gaacgtcatc atgagcacgg cggcggcaat cgcgccgatt gagccgagaa ggtcgctcaa
     6601 aacgtgcaga tacgcgccgc gcatgttgac gttggcttcg gtgtcgctgt ttttcagcat
     6661 gtaaaccgcc acgccgatgt tgaccagcag gccgacgacg ctgatgacga gcatccccag
     6721 cgtggcgatt tcgggcggat aaagcaggcg tttgacggct tcgtagaaaa tcagcacggc
     6781 gatgaccacc agcgacaaac cgttgaacat ggcggtgagg atttcgaacc gtttgtagcc
     6841 gaaggtcttt tgcagtgtgg tttctttttc gccgagtttg aacgcccaca acgccacgcc
     6901 caaggaaaat gcgtcgctga acatatgccc tgcgtcggaa agcagcgcga gcgaatgggt
     6961 cagccagccg ccgaacgcct ccagcagcat aaaggcggcg atgatgatga acgacacgcg
     7021 caatactttt ttgttggcgg tatgcgtgtg ggcgtgtgcg tgatgatggt gttcgtgatg
     7081 gtgagacata acgttcctca acagaggccg tctgaaaatc cggattcagg gttttcagac
     7141 gacctttttg ccttgtttca aactctgctc gaaatcttcc agcatatcca attcaaagcg
     7201 gctgaagccc gctttttcgc gcgcttcgat gttcacatag ccgcggaaga taaacatatc
     7261 gtaacgggca atcagactgc ggaacagggc aatcggttcc aaaccgcgtt cgcggcaaag
     7321 gtgttgatac caatggttgc cgatggcgac gtgtcccact tcgtcgctgt aaatgatgtc
     7381 caacacgccg caggtttccg aatcgccgcg ctgcgccacc ttcgcgcgta tccccggcgt
     7441 aacgtccagc ccgcgcgctt ccaacacgcg cggtaccaac gccatacgca acagcgggtc
     7501 gtaggcggtt ttgtacgcca tatcccacaa atggttgtgc gcctcgaaac tgccgtaatc
     7561 gaagccgaaa gcgcgcagcc tttcgcgcat caggcggaaa tggtacacct cttccttcgc
     7621 cactttcacc cagtcgcgga caaactgaaa cggcagcgtg cggaaacggt atgccgcgtc
     7681 caaagctaga ttgacggcgt tgaattcgat atgcgcaatc gcgtgcagca tcgccgcata
     7741 accttcggtt gtgttcattt tgcgcggggt cagttgcgac ggcgcgacca aaacaggctt
     7801 gtccggccgt cccgctcggg ggaagtccgc cggcggcgcg tttgcttccg ccccgtccgc
     7861 attttgaacg gcggcaaacg cctcatccgt cagccgtcct ttttcatctg ggtcgcccga
     7921 aagcagggcg cgttccagca aagcataaat atcgagtttc atctcaagtc cgccgtgttc
     7981 ggaaaacaaa tattatagcg ttttaaaaaa acaagatgaa gcatataatc tccgcgattc
     8041 ggcattccgc gcccaaaccg tcaaatatag tggattaaca aaaaccggta cggcgttgcc
     8101 tcgccttagc tcaaagagaa cgattctcta atgtgctgaa gcaccaagtg aatcggttcc
     8161 gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
     8221 acgcggcaca cattaaaggg cagcgtggcg cgcctgtctt ttccgagcgg tcaaaaaaat
     8281 cagccctcgg aaaacgcggt ttgcaaaatg caaaccgccc gtaacgccgc ccatatgatt
     8341 gttttgccgc gccgatactt tacgccacac ccatcccgac aaggaaaaat aatgatgaaa
     8401 ccgcacaacc tgttccaatt cctcgccgtt tgctccctga ccgtcgccgt cgcttccgca
     8461 caggcgggcg cggtggacgc gctcaagcaa ttcaacaacg atgccgacgg tatcagcggc
     8521 agcttcaccc aaaccgtcca aagcaaaaag aaaacccaaa ccgcgcacgg cacgttcaaa
     8581 atcctgcgcc cgggcctctt caaatgggaa tacacttcgc cctacaaaca gactattgtc
     8641 ggcgacggtc aaaccgtctg gctttatgat gtcgatttgg cacaagtgac caagtcgtcc
     8701 caagaccagg ccataggcgg cagccccgcc gccatcctgt cgaacaaaac cgccctcgaa
     8761 agcagctaca cgctgaaaga ggacggttcg tccaacggca tcgattatgt gctggcaacg
     8821 cccaaacgca acaacgccgg ctaccaatac atccgcatcg gcttcaaagg cggcaacctc
     8881 gccgccatgc agcttaaaga cagcttcggc aaccaaacct ccatcagttt cggcggtttg
     8941 aataccaatc cccaactctc gcgcggcgcg ttcaagttta ccccgccaaa aggcgtggac
     9001 gtgttgagca actgatgccg tccgccccga tgccgtctga atgccgccga ggcttcagac
     9061 ggcattttca cgcaggcgga acaatgtccc gcattaccgc ccgatcgggc atcggaacgg
     9121 caaaccggtg aaaattaacg gttgcgcccc ggctgttttt gccgtttaat gcaaaccttg
     9181 ctgcaccaag ggccaagaaa gccgaccggc cgcccccaca gcttccgatg caggcggcca
     9241 ggccgtccct gcaatgtttt ttatttttga acgaaaggtc gaaaaccatg aaaaaaacac
     9301 tggtggcggc ggcaatcctg agcctcgcct tgactgcgtg cggcggcgga aacgataccg
     9361 ccgcccaagc cccctccgcc aagcccgaag ctgaacaatc gggcaaactc aacatctaca
     9421 actggtcgga ttatgtcgat cccgaaaccg tcgccgcctt tgaaaaagaa accggcatca
     9481 agacgcgttc cgattattac gacagcaacg aaacactgga ggcaaaagtc ctgaccggca
     9541 aatccggcta cgacctgact gcgccgtcca tcgccaacgt cggccggcaa atcaaagcgg
     9601 gcgcgtatca gaaaatcgac aaggcgcaaa ttccccatta cggcaacatc gataaagatt
     9661 tgttgaaaat gatggaagcc gtcgatccgg gcaacgaata cgccgtcccc tatttctggg
     9721 gcatcaacac cttggcaatc aacacgcggc aggtgcaaaa ggcattgggt acggacaagc
     9781 tgcccgaaaa cgaatgggat ttggtgttca accctgaata caccgccaaa ctcaaatcct
     9841 gcggcatcag ctatttcgac agcgcaattg aacagattcc tttggcgttg cactatttgg
     9901 gcaaagaccc caacagtgag aatcccgaag acatcaaagc cgccgtcgat atgatgaaag
     9961 ccgtccgagg cgacgtgaaa cgcttcagct cttccggcta tatcgacgat atggcggcgg
    10021 gcaacctgtg tgccgccatc ggttacggcg gcgatttgaa cattgccaaa acccgtgccg
    10081 aagaagccgc aaacggcgtg gaaatcaaag tattgacccc gaaaaccggc gtgggcgtgt
    10141 gggtggattc ctttatgatt ccgcgcgacg cgcaaaacgt cgccaacgcc caccgctata
    10201 tcgactacac gctccggccc gaggtggcgg cgaaaaacgg cagcttcgtt acctacgcgc
    10261 ccgccagccg tcccgcgcgc gagctgatgg atgaaaaata cacctccgac gcatcgattt
    10321 tcccgaacaa agaactgatg gaaaaaagtt tcatcgtatc gcccaaatcc gcagaatccg
    10381 ccaaactggg cgtgaagctg tggcaagggc tcaaggccgg aaaataaccg caacccctgc
    10441 cgtctgaaac ctttcgggcg gcaggaaacg gcgcgtccgt tatcaaacag ggggggcgtt
    10501 tcccctcctg ccggttatga ttgggttaag attaaaatga tttagtaaaa tgagaaagat
    10561 atggatttaa gtatcgtagt tcccatttat aatgtcgaaa gttatttgga agcgtgttta
    10621 agttccatag aacctatatt aagtaatgaa aatgtcgaac ttatccttgt gaatgacggg
    10681 tcaaaagacg gaagtgaaga tatatgttat aaatatatag ataaaata
//
LOCUS       DAWHRS010000061        10666 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWHRS010000061 DAWHRS010000000
VERSION     DAWHRS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.061"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            389..1105
                     /locus_tag="WIB82_001104"
     CDS             389..1105
                     /locus_tag="WIB82_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3842410.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1208..1633
                     /locus_tag="WIB82_001105"
     CDS             1208..1633
                     /locus_tag="WIB82_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842411.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            1877..2077
                     /locus_tag="WIB82_001106"
     CDS             1877..2077
                     /locus_tag="WIB82_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842412.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2145..2504
                     /locus_tag="WIB82_001107"
     CDS             2145..2504
                     /locus_tag="WIB82_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842413.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVPNTSSTLKMVRSN"
     gene            2529..3383
                     /locus_tag="WIB82_001108"
     CDS             2529..3383
                     /locus_tag="WIB82_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ3842414.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTLLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3453..3668
                     /locus_tag="WIB82_001109"
     CDS             3453..3668
                     /locus_tag="WIB82_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842415.1"
                     /translation="MTITEEYSIDIRVTAEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRQALICLTKDNLKNAA"
     gene            3703..3963
                     /locus_tag="WIB82_001110"
     CDS             3703..3963
                     /locus_tag="WIB82_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842416.1"
                     /translation="MKYLIRTALLAVAAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            3960..4253
                     /locus_tag="WIB82_001111"
     CDS             3960..4253
                     /locus_tag="WIB82_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842417.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4256..4447
                     /locus_tag="WIB82_001112"
     CDS             4256..4447
                     /locus_tag="WIB82_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842418.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4447..4587
                     /locus_tag="WIB82_001113"
     CDS             4447..4587
                     /locus_tag="WIB82_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ3842419.1"
                     /translation="MDTLLKIIIALSFSGAEALAVWLFITAADAVLRRFPKPSHYRRV
                     KK"
     gene            4584..4793
                     /locus_tag="WIB82_001114"
     CDS             4584..4793
                     /locus_tag="WIB82_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ3842420.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(4851..5768)
                     /locus_tag="WIB82_001115"
     CDS             complement(4851..5768)
                     /locus_tag="WIB82_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ3842421.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMGRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(6634..7113)
                     /locus_tag="WIB82_001116"
     CDS             complement(6634..7113)
                     /locus_tag="WIB82_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ3842422.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(7549..7929)
                     /locus_tag="WIB82_001117"
     CDS             complement(7549..7929)
                     /locus_tag="WIB82_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ3842423.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(8084..9280)
                     /locus_tag="WIB82_001118"
     CDS             complement(8084..9280)
                     /locus_tag="WIB82_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEZ3842424.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVAR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRLLARTYLREVLKVDNDVAEKLLAHSLGTRTQTAYNRSELWEERKDALERWGNDVLR
                     LAKNGK"
     gene            complement(9472..9561)
                     /locus_tag="WIB82_001119"
     tRNA            complement(9472..9561)
                     /locus_tag="WIB82_001119"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9525..9527),aa:Ser,seq:cga)
     gene            complement(9639..9729)
                     /locus_tag="WIB82_001120"
     tRNA            complement(9639..9729)
                     /locus_tag="WIB82_001120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9693..9695),aa:Ser,seq:tga)
     gene            complement(9812..10591)
                     /gene="yaaA"
                     /locus_tag="WIB82_001121"
     CDS             complement(9812..10591)
                     /gene="yaaA"
                     /locus_tag="WIB82_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ3842425.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLNTDQIQ
                     YLQGRVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
BASE COUNT         2911 a         2762 c         2433 g         2560 t
ORIGIN      
        1 atattaaaaa ttatcgtcat caatgcggct tccccagccc gcggcgagct ttccacgaca
       61 ggcttttttt atttgggttg gtcgtctggt ttctttgagt tcaaaaacag gtcgggatac
      121 ttaagtttta ttcgtgcagg tatcccgcgc ttcgtccaat tgaaaacgca ttgagggcta
      181 ttccctgtta ttcgaccaac ttccgcgtaa ctgccgattg attgcaacag gcgtttgtct
      241 tcattgactc ttttatccat aaataaacca aatgtttaaa tctaatgcta atattaaaca
      301 ctatgtttag ataaaaatca agtcttgtgt aacaacattt tgtttaaata tgggagaata
      361 attaagccaa ccgcgaataa gattaaaaat gacaatgcac gagacaactg acagactttt
      421 tgagatagcc aaagagcagg gagttttaaa gccggctgac atagcagagc gtctgaatat
      481 cagccaacag gctttgaaaa actgggaaag tcgtggcata gcggcaaagg cgctgcctga
      541 agtagcaaaa gcattcggtg tatctgaaac atggctgaga acaggtgaag gcagccgaac
      601 cgcgcccgtc cttattgacc ccgacctacc ccacgaagtc aaagacatcc accgcccgat
      661 gacgtggagc agcaacgacc cgctgcccga cgatgattat gttttcgtcc cctacctcaa
      721 agagagctgc ttcaaaggcg gagtaggcac gtatgaaatc cccgattaca acggctaccg
      781 cctgccgttc ggcaaatcca cgcttaaacg caaaggcatc aatcccgaca acgtgttttg
      841 ctgcaccctg accggcgaca gtatggagga aaaaatcgca gaagacgcgg caattgccgt
      901 agatacgggc gaaaccgcca tacgcgacgg caaaatctac gccttcgccc aggacggtat
      961 gttccgcgtg aagtacctga tacggcagcc tggcaacagc gttctgatac gcagccacaa
     1021 cagcggtttc tatggcgacg aaaacgcccc cttggacagc ctgaccgtta tcggcagggt
     1081 attttggtgg agcgtgctgg attgaaaaat gttgctatta cgacgagata atattgatag
     1141 ggctttcaag attgtcaaaa ataggcgttt tgattcccct tggtggcctg gtgagtacga
     1201 tgccggtatg aatttccttg gcgtacaagg agagttgaag gttcacgagc tgcatcacag
     1261 aacagcaacc ctgtgctttg agtggcttgg cgaagtatct gcgccgcgca gaaaagagaa
     1321 ctacaaagac ctcaagccaa atgtattgta tgactttgat ggttccggta agcattttgc
     1381 caaccctgac gcaaggtatc tattgcccgt cggttcaagc ggcttaatct taaaacatat
     1441 ccaaatcgat gacgaagaca ctctgttgcg gctatggtgc gcacgaaata tcccaatgcc
     1501 acaccggcta tctaaaatcc cgatgcttcg gcagtattat ctaagcaagg catggcacga
     1561 aatttatgct atcaaccaac acctaaggaa aacgaagctg atagttgatg tggcatacga
     1621 ccccacagat taaacaaccc gcccgcatta tgcgggcttt tttcatgccc cgccgaacct
     1681 gaaaacaaca caaaaccgac aaagccgcct taataggcgg tttttttgcg cctgcgaaat
     1741 ctcaaaaata aattctttta ataatcaaaa tgtttataaa tcttaaacaa aaatcttaaa
     1801 cagattgttg catattatta aacgctatgt ttaaatacac ccatcgaaac aaacaacagc
     1861 cacatggagt accgacatgg aaaccatcac catccctaaa gaaaatttcg acgctttgct
     1921 aaatctcgca cgaaccgccg tcgccctgaa cgaaaatttt attccaaaaa taaattacgg
     1981 agcatccacc ataccagcag ccggctttgc cgcacttaac gagtttggaa tagctcgctc
     2041 caccgtggaa tctttggaaa ttctcaaaat taaataacct cctcggtatt gtcatcccgt
     2101 gataccggca acctaactaa cccctcttta aaggaaaatc aaaaatggaa acccaagaaa
     2161 agaaatttgt tcctgcctcc aagcaaatac cgaccgtaaa tagcggctgg ttagaccgtg
     2221 aattaggcga agccatgagt gaagcggtgc gtgcctgcct tgctcacggt aaacagtcag
     2281 aaattactgt caagctcaaa atccaacccc aaaacatcca aagcggaacg gtaaaaatca
     2341 gccacgatgt agcaaccaaa ctgcccaaag aaaaacgcga aggcggcatc gtctttgcta
     2401 cacctgacgg caacatccaa gccgacgatc cggcacaagg aaaattgaaa ctcaaacaag
     2461 ttcccaatac gtcaagcacg ttgaaaatgg tcagatccaa ctaactcaaa cccaaaagga
     2521 aatctaaaat ggaaacccaa gaaaacatga ttaaaaccgc cttacaagca gctcaaaaac
     2581 ctttttttga gtttgcaccg aacaatactc tgcttgtatt cacaccagac caagacggtg
     2641 gctggagata caaatcacac cccgaattga tgcaaaaccc gtaccgcaag tgcggcaaat
     2701 tcctcatgca cgataccgcc agtcttatta agtttgtaca aaaacacaaa caggacggca
     2761 cacaaatcta cattgatgcc gatttcaaat cagggcgtat tgatgtcacc gccgtcatca
     2821 acggccatac gcaacaagct ccgggctggc tcgattttta tgccgtatac accccgcaac
     2881 acactacatc ggcatccaac tggctgaaca acaatgcgca ccgcatgaat cagatggagt
     2941 tttcccattt cctgaccaac aatgcccgca acatcgtatc caaaaatccc ggtaacgaaa
     3001 attccgttta cccgaccgcc gccgaagtat tggatttcgc gctcaatctc gagtacaccg
     3061 aaaaaaccac cttcaaacaa ggctatcgtg aacaggacgg cagaatcaac tttaccttcc
     3121 aatccgaaga ctcagggcaa acagagaaaa acctcaaaat gtttgaacgt ttcggcatcg
     3181 agttcacccc gcatcaaggc ggtgcatcct actttgttga agccctgttg aaattccgca
     3241 tcgacaaaaa cagcggcgca ctcgtcctgt ggtacgaatt gcagcaaatc gatgctgtaa
     3301 tcgaacaagc ggcaaaagac atttccgaag ccgtacaaaa agccttccct gaaatcgaca
     3361 tctatttcgg cgtaatcgcc taaaaaaagg tcgtctgaaa ttgtgaacct tcagacgacc
     3421 tccacaaaaa cccaactccg aaagacccca aaatgaccat cacagaagaa tactccatcg
     3481 acatccgcgt aaccgcagaa caggggaaaa acgactacgg ctaccccacc gaacgctacg
     3541 gatgcgacat catcaacaca gacggggagc tgcttgtcgg catagagcca gagtacaaaa
     3601 ccccctttgc cgccgtcaga caagcgctca tctgtctgac aaaagacaac ctcaaaaacg
     3661 ctgcctgata ttaagccgtt accccaataa tgaggaatca aaatgaaata cttgatccgc
     3721 accgccttac tcgcagtcgc agccgccggc atctacgcct gccaaccgca atccgaagcc
     3781 gcagtgcaag tcaaggctga aaacagcctg accgctatgc gcttagccgt cgccgacaaa
     3841 caggcagaga ttgacgggtt gaacgcccaa atcgacgccg aaatcagaca acgcgaagcc
     3901 gaagaattga aagactaccg atggatacac ggcgacgcgg aagtgccgga gctggaaaaa
     3961 tgaactttca ggaagaaaac gagaacatca agaatgacat agagattaat ctcttgcatt
     4021 ccgctgaagc cgacagcgtg gaggcaatga tggacttggc ggtttacggg ctggcggcga
     4081 tggttgccaa acacaacaaa acgcgcggcg agcagcttgt gttacaggcg attgccggtc
     4141 atatgcggaa gcttttggcc gcgcttcccg aaaccgagtc attggtgcag accgggaagg
     4201 tttaccgccg acttgaagat ttgattttta aaacgtattt gccggagcag taaagatgaa
     4261 cacgaatcaa gatacgattc catttggcgg caaccttgtg atttgctgtt cgacgggaaa
     4321 cggcggggac gggcggtttt cttgtctgat taccgatcag ataccggatt taaatcttgt
     4381 cagaagcggg caggcgttcc cgatgttcat ttatgagtac gaaactagag aaaaaaggaa
     4441 agcctgatgg acaccctatt aaaaatcatc atcgcgctgt cattttccgg cgctgaggca
     4501 ttggcggtat ggcttttcat cacggccgcc gatgccgtat tacgccgctt cccgaaaccg
     4561 agtcattaca ggagagtaaa aaaatgaaca tcaacgaact gggcgcaagg atagaccgcc
     4621 cgaccatccg cgaactgatt gcctacgcaa cctgccgcaa ccgccccatt tcaaactcga
     4681 cactgctgcg tatggaaaaa gacggacgga taccgtgccg tctgaaaacc ccactcacat
     4741 cccccgtatg ggacacccgc gaagttttgg aagccctggg gttacagcaa taaaaaaacg
     4801 cccgattagg gcgttttcac attcagacgg cttttgtatt ccctactgca tcaaaccgac
     4861 gacaggttgc ggatttcggg cagcatcggg cggatttttg ccgcgtgttc cgcgtcggcg
     4921 tgtgcgttta aggcttcgag ggcgtttgcg gcggctttga ggcggctgcg tgtttccgcc
     4981 cagaccgtcc acatcgttac cgcctgtttg cagccgagct gcttcagcgg cagggaaacg
     5041 tctctgccca tttggatggc ccacgcgccg tatctgacgg caacggcgag gtcgtacagg
     5101 gcgttgccgc tgatgggcag ggcaggttgc ggtgcgggca atggttcgcg gtcgaggact
     5161 tcgccgtaca gcacgccgtt ttcaactgcc acgcggccca tcacgcttaa aacttcgggg
     5221 aactgttcgg caggtacttc tttgtagctg catccgaact tgcttttgac ggcagaccaa
     5281 agggtaatgg cgatacgcgc ctgcgcttct ttgggtgcgg atttggtcag ggcgttgtgc
     5341 agttttttga cggcttcgat ttgttcgccg gtcaagccgt ccggaagggc gcgggtgggt
     5401 ttgggggtaa ctgtttttat gccgtctgaa attttgccgt taagtaaaat ttcaatctgc
     5461 tcatcgcacc aaaccgcaaa tttcggatta agccaacggg caaagtgaat agcgagtttg
     5521 ggatgcagcc atgtgccttg ctcactgcca ccacgcttca cgataactat ttgatttgct
     5581 tccgttagga tttttctcct aacgcttaaa ttctcagcaa gtgcagagat atattgttga
     5641 gtttgttcac tttttaggta gtctttaggt aacttgccaa agtgagatgc aatggcggtt
     5701 gcatttaaaa aaccgtcttg acggaaagat acaggggtgt taccaaaatt gagaacagat
     5761 acgttcatga tagtttccta gtttagtttc gaaagaccca aatgggtggt cgggaggttc
     5821 gaaaacctac tagaaaaggc cggcgttatt ccccttgcgg gtattgtatt ccgcaccctc
     5881 ccgaccatag gaaactagtt cggatatatc gaaacaaaca accaaacaag aaaacttatg
     5941 gacgtaaaaa attcacgctg acggggtgaa tgccgttcta gtagaggttt tcgacgcctc
     6001 gtgtttcgta atatatccaa tctttctatt ttctgcaagc ataaaaaagc cgccatgtgg
     6061 cggatagggt cgcggtcttc aacagcatgt aggatttagg aatgcccaaa ttgggcgcag
     6121 gggtgttgaa aacacgcaaa cagacgcgcc gccagcctta cgggtaggcg cacccccaca
     6181 taggagtaaa tcgatttgat acgtatagac gtaaaaaatt ccactctatc gtgttggata
     6241 tacgctgttt gtcaggtgtt ttcaagcacc gtgggaaata ttatagagtt atggaaatgt
     6301 gtcaagagaa ttagcccaaa tgggcggacg cgcggttaag aacccacata ggagggcgga
     6361 cttattcccc tttcgggtct tgtattcgtc gcccacgcgg tcatagaaac ttcctgctat
     6421 cgaaacaaac aacaaggaaa gaaaactata gacatgaaaa aatcacattg acggagtgat
     6481 tgccgctgta gtgtggtttc tgacgccacg aacaggaata taaaacaaaa cccctgcaca
     6541 tgcaaggggt ttcccaaaaa accgtaggcg gcaaactgaa aggccgtctg aatttcagac
     6601 ggcctatgtt gcggcggatg ttaagctatt aagctatttg gttttcagag gctttttctt
     6661 ccattttcgg gctacggctt caaattcttg aaaggccgtc ggtgcgctcc tgatacggcg
     6721 tatttcaaaa atgaagctgc ttaggttgtc ccagtcacgt aacagcatgg agtattcgaa
     6781 atctttatac attttctcgt gcagtgctcc gcccaatacg cccgcacaga caaattcgcg
     6841 ctggtttaaa actgtcagta tggcttcacg gtctttcttc ttgtccgggg tatccgatgt
     6901 ataggtggcg agtatgcatc cgtctgtttt tgccagcccg tttactatgg ttatggcttc
     6961 ttgaagggcg gcattgttac gttcggccat gatcatgtcg atggtggcgc gctttttggc
     7021 gttttctgta ttcctcatga tgccgtaagc ggcaacaaaa acgccgataa cagtcaatat
     7081 cggcgttgct atctgtatta ggttgtcagt cattgccgtc ccagccgtct gaaaggcgga
     7141 aatcacggct ttccgaataa atatgtccca ttgtcatccc cttaagctga tgttgaaaat
     7201 aaatccttgc gtcggaatat acggcattgc ttgactattt tcaagattct gtacctcgtg
     7261 aaccggacta tacaaaaaaa ccgccttcgt gtaaagcggt tcttaaatcg cccgttaagg
     7321 cggacacaac gatagccaaa ctggaacagg aggcggcaaa ggcgggtttg tccaaaacag
     7381 ccttgattga aaggctgatt ggggatttgt aggcgtttgc agatacgaca aggggcgcat
     7441 tccgcgcccc atcacacgca cggacggctt gtcatcgcct gtcgcccgtg cgcggctgcc
     7501 catatttcag aacagtcttg caagcccctt taagggaacg gttttattct agtacagttt
     7561 gaatgccttg gcaacggctg ttttaatcgc ctgaaaatcc tcttcactga ttattggaat
     7621 gcagcggtcg cgccctttgg gtttgtatcg gtctaatcgt gccaatccga ctgttgccgt
     7681 catgtcgcat tttgcccaac attggatgtg cggcttgtcc ggtaaggggt ttccactcat
     7741 tttgtggtgg tagtccgcca aagggacagg ttctgtgctg cttaagggta cgaccgttac
     7801 cagtttgccg ttgtgcctgt ttcgcgctat gacgacgaca gggcgtttct tgaccatttc
     7861 cggttcttca taaccgcgaa agtcgcacat gataaccgaa cgttcccttg gttggaattt
     7921 taaaggcatt agccgctcct gacgatgaca ggctgctatt atagcagttc ttacacaaaa
     7981 aaccgccttc gtgtaaagcg gttgcaaaaa agccttccaa taaaaatgcg gaaacggttt
     8041 tttatttgcg tttccgcata atacaaacac acacacaaaa aaatcatttt ccgtttttgg
     8101 caagcctcaa aacatcattc ccccaccgtt ccagcgcatc cttacgctcc tcccaaagct
     8161 ccgagcggtt gtaagccgtt tgcgtcctcg tccccaacga atgggcaagc agcttttccg
     8221 ccacatcatt atccaccttc agaacctcgc gcaaataagt acgcgcaagc aagcgcaaac
     8281 cgtgcgccgt cgtatccagc cccgcccgct gcatcgccac gcgcggactt tccttattga
     8341 tatgcccttg aaaattcaca ctttcaaaca caaaccgccc gttcacattc aacgcccgcg
     8401 cctgatccaa cacccactgc atcgcccgcg acagcggcac atcatgcccc cgccgctcct
     8461 ttttccgttc ggcggggata cgccaaacct ccccgtccaa ctccgaccac tccatcaacg
     8521 ccgcctcctg aatccgcgtc attgtcaaca gcagccaata gatacaaagc cgcgttacag
     8581 gatggatacg caccctgccc gcataaaccg caaattcatt gcgctgctcc aaaaaatcaa
     8641 tcaggcgcgg caattcagac ggactcaacg ccgccatatt ccccgttttc gcccgttcaa
     8701 acacacccga accgatttgc gcgacagggt tgatttccat cattcccgaa ccgaccgcaa
     8761 acgcaaacac catcttcaga ctgtttttcg ttttgcgcaa cgtatcaacg ataccgcgcg
     8821 cctccatcac acgcagacag ccgaccacat ccgccgtcct gacttgacga atatcaagat
     8881 tgccgatagc cggaaaaacc caccgctcaa aattccgcat aacctgtccg gcatactttt
     8941 cagaccgccc cttcgaccaa cgcacaaacc aatcacgcgc cactttctca aaagcaaaat
     9001 ccgcccgcac cttcttattg acgacatttt ccccgtgcgc ccgttttcgg cgcacctcct
     9061 cccgccattc ccgcgcatcg gccagcgaaa aatcaggata ccgccccagc gaaattgtct
     9121 gctgccttcc atcctgcaca aacgacagct tccaactccg cccgcccgtc gggtagaccc
     9181 acaaagccag cccgcccccg tccgacaact tatacagctt atcgcgcggc ttcgcatttt
     9241 taacctgatt tgccgacagc ggcgtaatga ttttcgccat tatggtaatt tcccatttat
     9301 ccaaaaatta ccatacacat taccataaaa aaaatgcaat aagaagatgc cgcaagattg
     9361 caaatgattt actttgacgc gttttgatag ggcaaaccga tgatttttat atggtaattg
     9421 acacaacttg aacaaaaaag aaccgccctg aatcagggcg gttttgtttt ctggcggaaa
     9481 cggtgggatt cgaacccacg gaggatttgc accctcagcg gatttcgagt ccgctgcatt
     9541 cagcctctct gccacgtttc cgataatgaa gtaaaaccaa ataaaaatgc cgatgcttta
     9601 tgctacttca tttttatttg gtttttgacc tcaaggattg gcggaagcgg tgagattcga
     9661 actcacggag ggctatcaac cctcgacggt tttcaagacc gttgcattaa accgctctgc
     9721 cacgcttcca tcttcttgaa gaagacgaat aataatgaag tttatacatt ttgccaagca
     9781 cttttttcgg aaaatactta atttattgtt ttcactttat ttgttccgaa cgcataaaca
     9841 cccattcact ttcattggaa gccgcgtcat tgaatgcgta gccttcgtaa ttaaaatttt
     9901 tcagcatttc aggatcggta atattgtgtt ctgccgcata gcgcaccatt aatccgcgcg
     9961 cgcgcttggc atagaaactg atgattttat atttaccgtt tttttcgtct ttaaatatcg
    10021 gggtaatcag ccgcgcccga agtgttttcg tgttgacgga cttgaaatat tcctgtgagg
    10081 caaggttgac aagcgtattg ctgcctgctt gggcaagcgt gtcatttaaa aggttggtaa
    10141 tgatgtctcc ccaaaactca tacaaattct tcccgcgcaa attggcaaat gccgtcccca
    10201 tttccaaacg gtaaggctgt atcaggtcca gtggacgcag aaggccgtac aggccggaca
    10261 gcaggcgtac gcgcccttgc agatattgta tttgatcggt attcaatgtg tttgcatcca
    10321 taccttcgta aacatcgccg ttgaacataa agaccgcctg tttggcgttt tccggcgtaa
    10381 acggcgtgtt ccattctgca ttgcgctgcg cgtttaagag ggcaattttg tcggaaacgt
    10441 gcatcagttc ggcaatctgt tgcggcgcaa gctcgcgcaa ctgctgcatt agaatgtcgg
    10501 actctgccag caggtcgggt tgggtaaact cgctgacagg ggcagggtct ttttcattaa
    10561 ggttcttcgc aggggaaagg acaaaaaaca taatctgctc ggtattgtgt caaacacggg
    10621 gattttaagt ttaaacgtgt ttgcgggcaa gccgattaaa agtgaa
//
LOCUS       DAWHRS010000062        10609 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWHRS010000062 DAWHRS010000000
VERSION     DAWHRS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.062"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..467
                     /locus_tag="WIB82_001122"
     CDS             <1..467
                     /locus_tag="WIB82_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEZ3842426.1"
                     /translation="ALCRDQGLEMAYVKPHGAMYNQAAKNRALADTVARIVADFDPKL
                     KLMALSGSLLLEAGKAAGLGVISEVFADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVL
                     QMVRDGRVKAVNGSLVAVQADSICLHGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            596..1789
                     /locus_tag="WIB82_001123"
     CDS             596..1789
                     /locus_tag="WIB82_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ3842427.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            1897..2589
                     /locus_tag="WIB82_001124"
     CDS             1897..2589
                     /locus_tag="WIB82_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ3842428.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(2889..5579)
                     /gene="glnE"
                     /locus_tag="WIB82_001125"
     CDS             complement(2889..5579)
                     /gene="glnE"
                     /locus_tag="WIB82_001125"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ3842429.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRDTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTRETLKKLAELGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQRQRLAESMGFDDYADFLNAL
                     NQHRNQVNRLFNEILSEPGEQTQSNGEWQWVWQEKPDEEGRQCRLKEHGFDAEAVAAR
                     LDQIRHGHKYRHLSAHAQPRFDALVPLSVRAAAAQNNPTDTLMRLFDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPDAGDIYGRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGSPEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(5777..5980)
                     /locus_tag="WIB82_001126"
     CDS             complement(5777..5980)
                     /locus_tag="WIB82_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842430.1"
                     /translation="MGFWNGVAKAVGEGMIEAANEHKALKMEYAEKSSEELHEIVKSD
                     GFFKNSTREKGAAYAVLKERGEV"
     gene            complement(6117..6590)
                     /locus_tag="WIB82_001127"
     CDS             complement(6117..6590)
                     /locus_tag="WIB82_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ3842431.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(6643..6924)
                     /locus_tag="WIB82_001128"
     CDS             complement(6643..6924)
                     /locus_tag="WIB82_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842432.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(7011..7334)
                     /locus_tag="WIB82_001129"
     CDS             complement(7011..7334)
                     /locus_tag="WIB82_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842433.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(7667..8674)
                     /locus_tag="WIB82_001130"
     CDS             complement(7667..8674)
                     /locus_tag="WIB82_001130"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ3842434.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGRPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGGDSADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            8906..9142
                     /gene="acpP"
                     /locus_tag="WIB82_001131"
     CDS             8906..9142
                     /gene="acpP"
                     /locus_tag="WIB82_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ3842435.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            9298..10545
                     /gene="fabF"
                     /locus_tag="WIB82_001132"
     CDS             9298..10545
                     /gene="fabF"
                     /locus_tag="WIB82_001132"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ3842436.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         2438 a         2891 c         2750 g         2530 t
ORIGIN      
        1 aggccttgtg ccgtgatcag ggtttggaaa tggcttatgt caaaccgcac ggcgcaatgt
       61 acaaccaagc ggcgaaaaac cgtgcgctgg cggataccgt tgcccgaatt gtggcggatt
      121 tcgacccgaa attgaaattg atggcacttt ccggcagcct gctcttggaa gccggaaaag
      181 ccgcaggctt gggtgtgatt tccgaagtat tcgccgaccg ccgctatatg cccgacggta
      241 cgctggttcc ccgcagccgc cccgatgcgc aggtggacag cgacgaagaa gccatcgccc
      301 aagtattgca gatggtgcgg gacgggcggg tcaaagcagt gaacggcagc ctggttgccg
      361 tgcaagccga cagcatctgt ctgcacggag acgggccgca cgccgtggtg tttgccgaaa
      421 aaatccggca ggaactactg gcggcaggca tcaaagtttc tgcttgaaaa acagttcata
      481 ggccgtctga aaaccgcagg tttcgccaaa acgagcaaag cgagtttctg cgtagctaaa
      541 accgcaggtt tcgcccaaac acagcaacaa ccaattttat agaggataag cgattatgtc
      601 taatcaaaaa aaccgcagaa atgccttaat cggcgctgca ttcctgatgg cgacttccgc
      661 catcggcccg ggctttctga cccaaaccgc caccttcacc caagcactgg cggcaagttt
      721 cggctttgtg attctgctct cgattctgct cgacatcggg gcgcagctca atatttggcg
      781 gattgtcgcc gtttccgaaa aacaggcgca ggatattgcc aatcaggtct tgcccggcgc
      841 aggctatttc ttggctgtgc tgattgtgat gggcggtttg gcgttcaata ttggcaacgt
      901 cggcggcgca ggcttgggtc tgaacctgct gaccggactg tcaccggaaa ccggtgccgt
      961 gatcagcggc gtgattgcca tcggtgtgtt tctgtttaaa gaagcaggca aagtgatgga
     1021 caaattcgcc caagtgatgg gtttcgtaat gattgcgctg acggtttatg tggcatggca
     1081 ggcgaatccg ccgctggcag atgccgccgt gcataccttt atgccggaaa aactcgatgc
     1141 aatggcgatt gttacactgg tgggcggcac ggtcggcggc tacatcacct tcgccggtgc
     1201 gcaccgtctg ctggacgcag gcatcaaagg caaatcggcg ttgccggaag tgagccaaag
     1261 ctcggtgcgg gcgatcctga ttgcctcgat tatgcggatt gtattgtttt tggcggtttt
     1321 gggcgtggtc agccaaggcg tacagctcaa tcccgacaac cctgcttcca caccgtttga
     1381 atatgcggcg ggatacatcg gcctgctgat tttcggcgtg gtgatttggg cggcttcgat
     1441 tacttcggtg attggtgcgg cttatacttc ggtgtcgttc ttctccggtc tcagcccgtc
     1501 tatcgaacgc aataaaaaca aatggattat tgcctttatc gccgtgtcca ccgccgtatt
     1561 ttccaccatc ggcaaaccgg cgcaggtgct ggtgttcgta ggcgcattaa acggcctgat
     1621 tttaccgatt tccctcggtc tgattctgat tgccgcctac aaaaccaaaa ttgtcggcga
     1681 ctacaaacac ccgctgtggc tcaccgtttc cggcgtgatt gtggtcggtt tgatggcagt
     1741 actcagcgcc atcaccatca gcaaatatat cggcggcttg ttcggctgaa ccttgtagcg
     1801 cagatgtttg attttgtata ttatccgaag tttgccgcct gatatggaaa attcctgttt
     1861 cagacgatgt atccggtctg aaacccattc acaactatga aacgcttcac ctatactctt
     1921 tccgacggtt tgtgcatcga aatcgaactc aaacgcagtg ccaagaaaaa tctgattctg
     1981 cgccccgtca atatgcagac ggtcagcatc aacgtcccac ccttttttca agaccacgcg
     2041 ttagcaaact ggctggcggc aaacgaaacg attttgcgga acacgcttgc caaaacgccc
     2101 gtgcatcctg tttcccaccc aaacttaccc gagtggattt ggtatcgggg aataaagacc
     2161 aagctggata cccacagcca aagccatatc cgtatcacgt cgtctgaaat cctgcttccc
     2221 cgaaaagaaa ccgccgcaca aatcgaccac ctgcgccgcc tgttgaacga acgcgcccgc
     2281 gaatacctgc tgccccgcct tgaaaaacac gcagccgaaa caggactgac gcccaccgcc
     2341 acagacctga gcaacgccaa aaccttttgg ggcgtatgcc gcccgcacac cggcatccgc
     2401 ctcaactggc ggctgatcgg cacgcccgaa tacgtcgccg actatgtctg catccacgaa
     2461 ctctgccacc tccgccaccc cgaccacagt ccgcgctttt ggcatttggt gaacacgctg
     2521 acgccgcata ccgacaatgc taaaagttgg ctgaaggcgc acgggcggga attgtttgtg
     2581 ctggggtgaa ggctaaccgt agcgtgggct tcgcccgcga gaatccaccc cctcccgtac
     2641 ggagggagag ggttggagag agggtggctc ttgtcccttg ttctgtatat ttgcaagcca
     2701 ccctatccta gccttccccc gttggataag ggaaagggat caagctgctg taactgaaag
     2761 aggaatccgg tttttgggtt tcagccattc ccgataaatc gctttagctc tgccgcaacc
     2821 ttaaaacggc acggtacgaa aataccgtct gaaacccaga ttgtcaggct tcagacggca
     2881 ttttttgttc aggcggttgc cgcttcttcg ccgaacactt cccgccacaa tttcctgaca
     2941 ttgccgtaat gtgccaacag ttcgccggtt acttcggttt ttgccgcgtc gcgcagtttg
     3001 gtgttgtgct gctgccggcg gtagaagcgg taggcggtgc ggctttgtcc ggcaagcgtt
     3061 ttatcaatca aaccgcagtc ggcggcgatg ttcaagaggg cgatgttgcc gtagttgtcc
     3121 aagagttgcg gatattttcg ggcgtgggca agtatcagat attggacgat aaattcgaca
     3181 tcgaccacgc cgccgcgcgc gtatttgacg ttgctgtcgg caggcgggtg ggtggggaac
     3241 attttttcgc gcatttcgat gatttcgcct gccaaggcgg tttggtcgcg ttcggcggtg
     3301 aggatttcgg tgcggatgcg gtcgaatgcc gtctgaattt cgggcgagcc gcagataaag
     3361 cgggcgcggg tgagggactg gtgttcccac gtccacgcgt tttcgcgctg gtatttttca
     3421 aaggcggcaa tgctgtgggc gaggaagccg gcgtcgccgt tgggacgcag gcgcaggtcg
     3481 gtttcgtaga ggctgcccgc gccggtggcg gcggaaagcc aattggtcag gcggcgggcg
     3541 agccgtccgt aaatatctcc cgcgtcggga tgggggtcgt cgtagagata aaccaaatcc
     3601 aaatcggaag cgtagccgag ttctttaccg cccagtttgc cgtagccgat gacggcgaat
     3661 tgcggggtgt cgcggtgttt tttgggcata tccgcccacg cgcacgaaag ggcggcggcg
     3721 aggatggtgt cggcgagggc ggagagctgg tcggagaggg attctaccgt ccacagtccg
     3781 gcgaggtctt ggacggcgag gcggaagact tgggcgtgct ggaaatggcg cagggtgtcc
     3841 atttgcgctt cggtgtcgcc gccgcaggct ttgatgccgt ctgaaagttc ggcggcgagt
     3901 ttgtcccagt caaacgccgt atctaataat tgcgcgctga tgagttcgtc caacaaaatc
     3961 ggatatttgt tcagatacgc cgccacccaa gaactttggc ccataatctc tgccagttgc
     4021 gccagggttt gcggatgttc gttgaggaag gcgagatagg cggagcggcg gctgatgttt
     4081 tcgagaaagt caaacagccg catcagcgta tcggtcgggt tgttttgcgc tgccgccgcc
     4141 cgtaccgaca acggcacaag cgcatcgaaa cgcggctggg cgtgtgcgga aagatggcgg
     4201 tatttatggc cgtggcggat ttggtcgagc cttgcggcga cggcttcggc atcgaacccg
     4261 tgttctttca gacggcattg ccgcccttct tcgtcgggtt tttcctgcca aacccactgc
     4321 cattcgccgt tgctttgcgt ttgttctccg ggttcgctca aaatttcgtt aaacaaccgg
     4381 ttgacttggt ttcgatgttg atttaaagcg ttcaaaaaat cggcgtaatc atcaaatccc
     4441 atactttcgg caagccgttg ccgctgttcg ggcgaagccg gcagggtttg ggtttgccgg
     4501 tcgtcccaat attgcaaacg atgttcgaca tcgcgcaaaa agcggtaggc ggcgagcagg
     4561 gtttcgacgt tttcagacgg catgatgccc agctcggcaa gcttcttcag cgtttcccgc
     4621 gtgcctttca gttgcagcgc gcgcatttgc ccgccgcgta tcatctggaa aatctgggcg
     4681 ataaattcga cttcgcggat gccgcccgcg ccgagtttga tgttgtccgc catccctttt
     4741 ttgctgactt cgctgcggat ttggcggtgc agcttacgca tcgcctcata cgcgccgtaa
     4801 tccagatatt tgcggaacac gaaggggcgc accagcgatt tgattccgtt cggatacggc
     4861 gtgaccacgc ggcctttgca ccacgcgtag cgttcccatt cgcgcccctg cgtaatcagg
     4921 tattgctcca gcgcggtttc gctcaacacc agcgcgcccg aatcgccgtc cggccgcagc
     4981 cgcatatcga cgcggaacac ctgcccgtcg gcggtgatgt cgttcaacaa tgcaatcagt
     5041 ttctgcccga ctttggtgaa aaactcctga ttgccccgtt cgcgcctgcc gtcggtatcg
     5101 cccgattcgg gatagacgaa aatcaaatca atgtcggaag acacgttcaa ctcatagccg
     5161 cccgccttgc ccatcgccac cacgctcaaa tgctgcggcg atttggtatc acgcccgatc
     5221 ggcgtaccgt acatatcccg ataataggcg taggcgaaat ccagcgcggt attgacggca
     5281 aaatcggcaa acagcgtaat cgtgcgggtt acttcgttca aatcgctgat gcggtttata
     5341 tcgcgcacga taatctgcga caccacataa cggcgcaacc cgcgcaactg ccgcgccaat
     5401 tcttcctcgt tttcttccgc acggattttg tcccagtcgg caaaggcttg gaaatccgct
     5461 tcggtcaaaa ccttgtccag cataggcagg aaaatttcgg gcttgagttt gccgttgtcg
     5521 agctggcggg cgaggaagag ggaatggcgg cgggcggtgt cgaggcggtt gtcggacatt
     5581 tcggattccg tttgaaaaaa tggcaggaag atgatggcgg attcacttca aatcaggaca
     5641 gggcgagggg atgccgtacc ggtttaaagt caagccacta tatcatagca accttcgatg
     5701 ccgtctgaaa ccttgccccg acatccagac ggatgcctgc cgataaaaaa cagcggcaaa
     5761 tgccgccgtt tcctgttcac acctcgccac gctcttttaa aacagcataa gccgcacctt
     5821 tctcccgtgt ggaattttta aaaaaaccat cactctttac gatttcatgc agctcctcac
     5881 ttgatttctc cgcatattcc attttcaacg ccttatgctc attggctgct tcaatcattc
     5941 cctcgcctac tgcttttgcc acaccattcc aaaaacccat aatcatgtcc tccaatcgtt
     6001 tcaaataaat aaggtcgttg tttctgtcaa gaaacaacga cagtttataa actgtcggaa
     6061 aacaaaatgc cgtctgaagg ccggataatg cttcagacgg catcggattg ccggttttaa
     6121 ccctgccctc cgccgttgtt caaatagtgt tcccagtgag aatataaccg ggcaatattt
     6181 tcttcatagg tgtgggcaga aggaatataa ccgtctttgg gcggcatata cctgacaaat
     6241 gattctattt cttccggata atcaaaatct gcataaattt tttcaacttc ccccaaagga
     6301 tcctcaaatt cttcttttct atggaataca tcgcttaaga ggacgtacag ccaattttta
     6361 ggggaataat ccctcatttc cgaagccaaa tcttccagaa caggctttaa ttcaagaata
     6421 ttggatttgt tgattaaaga tagtttgaac acacgttcat catgatctga aagcgtcatt
     6481 tttcgggcat aagctgccac atcagcccaa cccaagtatt tattcccata cccccacaga
     6541 atatccttcc aagaaagacg gactttttgc gcggttaaat caaggttcat tttttactcc
     6601 gattgcctgc cccgtcattc cgccccttcc gattgacgcc tgttacgaaa agtcttccaa
     6661 atgacggctg attgcctctt caacagtcgg atgccaatcc ataaatgaag cctgcaattc
     6721 ctctaaaacc aactcattga ataaatcatc caaattccgc caatccgttt tttcatccgg
     6781 atagatgaaa tttgccataa cgacagtatg tttgttttgg gaataatccg ataaggcgca
     6841 aatcatcaga taccgccccg aatgctccag ataaaaaatt cgtttttcca aaggaaaccg
     6901 gttattccgt tgcccgtttt ccatttttca tcctttttaa cgtatcggct tccgcgtcct
     6961 tcaaattgag ttacctgcct tccgccaagc aaacgcccgc gcccctttca tcatttgaat
     7021 atccgtttga ccatatttac cagataacta tcttctttat ataaatcaat cagcaaaggc
     7081 acgatacccg gctcataaaa taattctgtc gtatcagatg tttcttttaa cgcggaaatt
     7141 atctccaaca gttcataccc gtatcgggga taagcaaaca aataggggac tgcttcccgc
     7201 aaacatgaaa atgcttcgta actttgcatt tcccatatat gttcatcttt aaaatcactc
     7261 aaataccatt catccaattc actttccgga tcggatatca atcggataaa ttcatttagt
     7321 tcgtccttaa acatatcaaa tccttaaata taaaatcttt tgattatccg ccgtatcctt
     7381 attttccaca tcatgaattt caacgccgtt catattttgc gttttcccaa gccgtccgcc
     7441 acagtataaa attatttttt gtatcaattg cttatatatt taacaactta ccgccggact
     7501 tgctttggct gcgtatcaag attgtccggt tcgcggcggg cgtttgcttt gtttcaaaca
     7561 gggcggcgcg taagggattt tcaaggggcg tcaggcggaa aaaggaaatg aaaatgccgt
     7621 ctgaacggca aaacgtgcgt tcagacggca ttttgggcgg atcgcgtcat cgcgccaaag
     7681 ccttcaaaca ttctttgacc aatgccggac ctttgtatat caatccgctg tacacctgga
     7741 cggcagttgc gcccaagcgg attttatctg ccgagtcccc gccttccata atgccgccta
     7801 cgccgataat cggcagcttg ccgtctatgt ggtctgccaa cagcttcaac acccgattac
     7861 ttttttcatg aacgggccgc ccgctcaaac cgccctgctc gcctgcgagc ggatggctgc
     7921 cgagacttga tttgtcgatg gtggtattgg tagcgatgat gccgtccatt tcgacggatt
     7981 tgacaacgtg ggcgatgtct tcgatttgtg cttcatccaa atcgggggcg attttgacgg
     8041 cgagcggaac gtatttcccg tgtacagagg caagctgtgc ctgtttgttt ttcaaagcct
     8101 caagcaatgc gctcaactcg tcgccacctt gcagcgcgcg gaggtttttg gtgttgggcg
     8161 aggaaatgtt gacggtaatg tagcttgcgt gcgcgtaggc tttttcaagg cagattaaat
     8221 aatcatcggc agcgttttcg atgggtgtaa ccgcgttttt accgatgttg atgcccaata
     8281 cgccactgaa tttacttttt tcgatgtttt gaatcatggc atcgataccg tggttgttga
     8341 aacccatgcg gttgatgatg ccttggtgtt cgggaacgcg gaagaggcgc ggctgcgggt
     8401 tgccgggctg cggtttgggc gttaccgtgc cgatttcgat gaaaccaaag ccgagcgcgc
     8461 ccaatgcgtc gatgtattcg ccgtttttgt cgagtccagc ggcaagtccg acagggttgg
     8521 gcaaatccat acccatcaat tttacaggtt tggtacggtt gccggttacg ggaatcagac
     8581 ccaatttata aaccgtgtag agcgcgtcga gcgtgaagtg gtgggctttt tcggcatcga
     8641 gtgcaaacag gatgcggcgg gcaagtggat acatgacagg gctttctgct tcagatggaa
     8701 atgccgccat tttaaccgaa atcccgccaa acatacggca cacaggtcaa acttgaacga
     8761 caaaacccgc cgcacccaaa aatgtacaaa tcggcaaata ttgatataat agtcattaga
     8821 tagaaaactc tattaaaagt cggtacaata ccaccgattt atttccctta aacaacctgc
     8881 cgcaaggcat aaaggaacga ctgatatgtc aaacatcgaa caacaagtta aaaaaattgt
     8941 tgctgaacaa ctgggcgtaa acgaagccga cgtgaaaaac gaatcttctt tccaagacga
     9001 cttgggcgcg gattctttgg acaccgtgga gctggttatg gctttggaag aagcattcgg
     9061 ctgcgaaatc cccgacgaag atgccgaaaa aatcaccacc gtccaactgg ctatcgacta
     9121 catcaatgcc cacaacggct aaccggtcgt cgcccgacac aacagcctct gctgcaccgc
     9181 gcaatagagg cttttccctt atatggcaaa ctgtttgaac ccctgccgca aattacagac
     9241 ggcatctgca cgacagccgc atatgccccc attccgacaa ccaacagcga ggttatcatg
     9301 agtcagagaa gagtagtcat tacaggctta ggtcaggttt cccctgtcgg caacactgtc
     9361 gcagaggctt gggacaccct gctcgccggc aaaagcggca tcggcgcgat tacccgcttt
     9421 gacgcatccg acatcaacag ccgtgtcgcc ggcgaagtgc gcggtttcga catcggacaa
     9481 tacatcagcg cgaaagaagc gcgccggatg gacgtattca tccactacgg cattgccgcc
     9541 gcattgcagg caatcgccga ttcgggcttg gacgatgtgg aaaacctcga caaagaccgc
     9601 atcggcgtga acatcggttc cggcatcggc ggactgccca gcatcgaggt caccggcaaa
     9661 gccgtaatcg aaggcggcgc gcgcaaaatc aaccctttct ttatccccgg ttcgctgatc
     9721 aacctgattt ccggacacgt taccatcctc aaaggctacc gcggcccgag ctacgggatg
     9781 gtttccgcct gtaccaccgg cgcgcacgcc atcggcaatt ccgcccgact gatcaaatac
     9841 ggcgacgcgg acataatggt tgccggcggc gcggaaggcg caatcagcac tttgggcgtg
     9901 ggcggttttg ccgcgatgaa agccctctcc acccgcaacg acgaccccgc caccgcttcc
     9961 cgtccgtggg acaaaggccg cgacggcttc gttatcggcg aaggcgcggg catattggtg
    10021 ttggaagaat tggaacacgc caaaaaacgc ggcgcgaaaa tctacgccga aatcgtcggc
    10081 ttcggcatga gttccgatgc ttaccatatc accgcgccga acgaagaagg ccccgccctt
    10141 gccgttaccc gcgcgctgaa agatgccggc atcaatcccg aagacgtgga ttacgtcaac
    10201 gcgcacggca cgtccacccc cttgggcgat gccaacgaaa ccaaagccct caaacgcgca
    10261 ttcggcgaac acgcctacaa aaccgtcgtc agctcgacca aatccatgac cggccacctg
    10321 ctcggcgcgg cgggcggcgt ggaggccgtg tacagcattt tggcgataca cgacggcaaa
    10381 atcccgccga ccatcaacat ttttgaacaa gacgttgaag ccggctgcga tttggactac
    10441 tgcgccaacg aagcgcgcga cgcggaaatc gacgttgcca tttccaactc cttcggcttc
    10501 ggcggcacca acggcacgct ggtcttcaaa cgcttcaaag gctgattccg caaagccgcc
    10561 gccgacatcg aaatgccgtc tgaaaccgtt tcagacggca tttttatag
//
LOCUS       DAWHRS010000063        10516 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWHRS010000063 DAWHRS010000000
VERSION     DAWHRS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.063"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1790)
                     /gene="mutL"
                     /locus_tag="WIB82_001133"
     CDS             complement(<1..1790)
                     /gene="mutL"
                     /locus_tag="WIB82_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ3842437.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPEQSLHERIAAIVGDDFQT
                     ASLEIDSGSGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDRASNYPTGNKPDTRNAFGASGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLNELAQVGSSQTIEEHENR"
     gene            1933..2118
                     /locus_tag="WIB82_001134"
     CDS             1933..2118
                     /locus_tag="WIB82_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ3842438.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            2129..2797
                     /locus_tag="WIB82_001135"
     CDS             2129..2797
                     /locus_tag="WIB82_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ3842439.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            2797..3513
                     /locus_tag="WIB82_001136"
     CDS             2797..3513
                     /locus_tag="WIB82_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ3842440.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            3561..4046
                     /gene="purE"
                     /locus_tag="WIB82_001137"
     CDS             3561..4046
                     /gene="purE"
                     /locus_tag="WIB82_001137"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ3842441.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(4229..5683)
                     /locus_tag="WIB82_001138"
     CDS             complement(4229..5683)
                     /locus_tag="WIB82_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ3842442.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(5680..6381)
                     /locus_tag="WIB82_001139"
     CDS             complement(5680..6381)
                     /locus_tag="WIB82_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ3842443.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(6378..7157)
                     /locus_tag="WIB82_001140"
     CDS             complement(6378..7157)
                     /locus_tag="WIB82_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ3842444.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            7442..8848
                     /locus_tag="WIB82_001141"
     CDS             7442..8848
                     /locus_tag="WIB82_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3842445.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLDMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQIAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWVFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVAGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGTNLTRNTPS"
     gene            8960..10486
                     /locus_tag="WIB82_001142"
     CDS             8960..10486
                     /locus_tag="WIB82_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ3842446.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPAPRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2266 a         2927 c         2835 g         2488 t
ORIGIN      
        1 cggttttcgt gttcctcgat ggtttggctg ctgccgactt gggcgagttc gtttaatacg
       61 tctttggcga gcgagacgac atcggctttg ccgagcatgg cgggaactgc acggacggcg
      121 agggtgttgc cgcccatatc ggataattcc agcccgaagc ctgccagcgt ttcggcataa
      181 tcggcaaggg cggcgcattc ttcgtgggac gcggcaaagg ttacgggaat aagcaggcgt
      241 tggctttgca gcttgccgtt ttcctgccgt tggcgtttca ttttctcgta gttgacgcgt
      301 tcggcagcgg cgtgcatatc gatgagcagc aggctgtctt cggcttgggc aaggatgtag
      361 atgcctagca attgggcaat ggcgaaaccg agcggcggca gttcggatgg ggacgggatg
      421 ccgtctgaaa gcggcgtatc tgtttgggga gcaggcgttt cagacggcat attgccgaaa
      481 cgtgcctgct cgaatcggct taactcaagg tcgatgtcgt cggttttttt gtagagttcg
      541 gcataggtgt tcattgccgc gcggctttcg cgcagggaca ggctgcgttg ttgcggcgca
      601 tatgcggact gatagggcat aggcgcggtt ttgcctgatg caccaaaggc attgcgcgta
      661 tcgggtttgt tgcctgtcgg gtagttggat gcgcgatcaa acagattttc gctgtcgttt
      721 tcagacggca ttggggcagg cgtaacgccg gtaatgtcat gcaacacttc gcctgcgttg
      781 ctgacgcttt cggtcaagtc ggcgcgtgtg tcggcaaggg ctttgttgag cgtgtggaac
      841 acgagttggt gcacctgctg gctgtcgcgg aagcggattt cggttttggt cgggtggacg
      901 ttgacatcca cggcttcggg cggcaggtcg aggaaaagga cgaaagcggg ggtgagggcg
      961 ttgtgcaaaa cgtcgcggta tgcctgcttg acggcgtgga gcatcacttt gtcgcggaca
     1021 aaacgatggt tgacgaagca gtattgtttg tcggttttgc ctttggcgaa agtcggcttg
     1081 gcaatcgcgc catagagccg caatgcgccg ctgccgctgt cgatttccaa agatgccgtc
     1141 tgaaaatcat cgccgacaat ggcggcaatc cgttcgtgca ggctttgttc agggagtttg
     1201 aacacttgtt tgccgtcgcg tttgagcgag aaggcaatgt gcggatgcgc cagcgcgagg
     1261 cgttcgagca tagtggcgca gtgggcgtat tcggtggctt cagacttgag gaacttgcgc
     1321 cgcgcggggg tgttgaagaa gagttcggcg gcttcgatgg tggtgccgac ggggtgggcg
     1381 gcggcggtgg ggctgctgag tttgccgtct tcggctttga cttgggttgc gtgcgaactg
     1441 tcttcttgac ggctggtcag ggtcaggcgg ctgacggagg cgatgcttgc caagccttcg
     1501 ccgcgaaagc ccatgctggc aacgtgttct aaatcgttta aggttttgat tttgctggta
     1561 gcgtgtcggt ggagcgcaag ctcgatgtcg tccggatgaa taccgctgcc gttgtcgctg
     1621 acgcgaatca ggcggatgcc gccgcccgcc agctcgacat caatcgccgt tgcgcctgca
     1681 tcgatactgt tttcaacgat ttctttcaag gcgttggcag ggcgttcgac cacttcgccg
     1741 gcggcgattt ggttgacaag atggtcgggc agggcggcaa tgcgtggcat aaggtaggct
     1801 tccgttgcaa aaacgcttta ttctataata aaacccctta cttttctgcc tgtattcttg
     1861 ataaaagccg tttatcccgt ttggaaaata ccagtataat cacgccgtat cccgtaaaat
     1921 tggagcgcaa agatgtatca ctaccaatcc gaagccacac aattcctcaa ccgcctgatt
     1981 gaagaaaaac ctgagttgga acagcagcgt ttggaaaacc gggggctttt gtgggatgtc
     2041 gaactcaatc ccgaagaaca ggaaaacttc gaggcggcaa aagtagcgaa aaaaccttat
     2101 acctattacc aagactgaac ccgccgaaat gacgacccat ctgtccaatg tcgcaccgga
     2161 cctgcaaaac tatctgaaca gcatcggcga acccgaacat cccgttttga cgcggctgcg
     2221 tgagaagacc gagcatcacc gtatgggcaa aatggcgatt gcgcgcgaac aggcggcggt
     2281 tttggtttgg ctggcaaagc tgattcgcgc ggaaaaatat ctggaagtcg gcgtgtttac
     2341 cggatacagc agcaccgcgc ttgcattggc gttgccagaa cacgggcgga ttaccgcctg
     2401 cgacatcaat gtaaccttta ccgatacggc gcgtcaggtt tggaacgagg caggtgtggc
     2461 acataaaatc agcctgcacc tgcaacccgc attgctgaca ttggatgatt tgattgcaca
     2521 gggtgaagcc ggaagctacg atttggcact gatagacgca gacaaaccgc ccacgccgcg
     2581 atatttcgag cgttgcctca agcttgtccg tcaaggcggc atcatcgcca tcgacaatat
     2641 tttgctgaac ggaagggtga tgcgcgaagc ggcttccgat gcgccgccca gcgtcggcat
     2701 cctcaaagat ttcaatcaaa acctgccgaa cgatacgcgg attgtcccca tcaccctgcc
     2761 cgtcggcgac ggcttgaccc tgcttctgaa aaaataatga agaccaaatt accgcttttt
     2821 atcatttggc tgtccgtatc tgccgcctgt tcttcccctg tttcccgcaa tattcaggat
     2881 atgcggcccg aaccgcaggc agaggcaggt agttcggacg ctattcccta tcccgttccc
     2941 actctgcaag accgtttgga ttatctggaa ggcacactcg tccgcctgtc gaacgaagtg
     3001 gaaaccttaa acggcaaagt caaagcactg gagcatgcga aaacacaccc ttccagcagg
     3061 gcatacgtcc aaaaactcga cgaccgcaag ttgaaagagc attacctcaa taccgaaggc
     3121 ggcagcgcat ccgcacatac cgtcgaaacc gcacaaaacc tctacaatca ggcactcaaa
     3181 cactataaaa gcggcaggtt ttctgccgct gcctccctgt tgaaaggcgc ggacggaggc
     3241 gacggcggca gcatcgcgca acgcagtatg tatctgttgc tgcaaagcag ggcgcgtatg
     3301 ggcaactgcg aatccgtcat cgaaatcgga gggcgttacg ccaaccgttt caaagacagc
     3361 ccaaccgcgc ccgaagccat gttcaaaatc ggcgaatgcc aatacaggtt gcagcagaaa
     3421 gacattgcaa gggcgacttg gcgcagcctg atacaggctt acccgagcag cccggcggca
     3481 aaacgcgccg ccgcagccgt gcgcaaacga tagtttttcc atttttccgc tttttacccc
     3541 atccgcacca aggaggattt atgatacaaa tcggcatcat catgggcagc aacagcgatt
     3601 ggcccgttat gcggcaggca gcgcagtttc ttgaagagtt cggcgtagaa tatgaggcgc
     3661 gcgttgtttc cgcacaccgc accccggatt tgatgttcca atacgccgaa accgcacggg
     3721 cgcgcggcat caaagccatc atcgcgggtg cgggcggcgc ggcgcattta cccggtatgg
     3781 ttgccgccaa gaccaccgtc cccgttttgg gcgtacccgt ccccagcaaa tacctgcgcg
     3841 gcgaagattc gcttttatcg attgtacaaa tgcccaaagg cgtacccgtc gccacattcg
     3901 ccatcggcga ggcaggcgcg gcaaatgccg cattgttcgc cgtttccatg ctcgccaacg
     3961 aaacccccga actggcgcaa aaactggcag acttccgagc caaacaggaa caggctgttt
     4021 tgagtatgga attggaacag atttaaaacc cgccgtccgc gaatatatag tggcttaaat
     4081 ttaaaccagt acggcgttcc ctcgccttgc cgtactattt gtactgtctg cggcttcgtc
     4141 gccttgtcct gatttttgtt aatccactat acaaatgccg tctgaagccc tgtttcgggt
     4201 ttcagacggc atttttgcga acaacttttt atgcctgttc tttttggcgc attttttctg
     4261 ccattagttc gtgcagggtt ttcttcgccg gtttcatcgg cacgcggttt tgcgtccaac
     4321 ccaactgttt gcgcggggtc aggttgcgga acttggtggc tgcccaaccg aaggcgcggt
     4381 aggttttgct gccgctgaaa ataccgttga atgtgcgcca cgccatttgt tcgccgaagg
     4441 tatgcgatgc gccttgccct cggatggggt gcggcacggt ttcggtcggc gaacgttgcg
     4501 cttcaacgcg caaacgctgc atttgttcgg taatcgggat gcgtaccgga cagacttcca
     4561 cacacgcacc gcacatggta caggcggtcg gcaggtcgcg ggtggcatcc agacccaaca
     4621 ggtgagggga aataatctcg ccaatcgggc cgggataggt tgtgccgtat gccgcgccgc
     4681 cgatgcgggt ataaaccggg caatggttca tacacgcgcc gcaacggata cattgcaggg
     4741 tgcggcgcat ttggtcttcg gcataagcct ggctgcggcc gttgtcgagc agaaccaagt
     4801 gcatttcttg cggaccgtct aattcttcac tgcggcgcgg gccggtaatc atattgaaat
     4861 aggttgtgat gttctgaccg atggcggagc gcggcagcag actgtacaag ggtgggatgt
     4921 cggacaattt cgccaccact ttttcaatgc cggtaatagc gatatgcacg ggcggaacgg
     4981 tggtactcaa gcgaccgttg ccctcgtttt ccaccagaca cagcgtacct gtttcagcaa
     5041 cggcaaagtt tacgccactc aagccgacat cggcagtgct gtaaatatcg cgcagggctt
     5101 tgcgggcgaa gccggtcagt tggtccacat cgtctgtaag cggtgtgccg aggttttggt
     5161 ggaacagttc gctcacctgt tctttggttt tatggattgc aggcatcacg atatgggtcg
     5221 gtttttcgcc tgccatttgg acgatgaact cgcccaagtc gctttctacc gctttaatgc
     5281 cttttgcttc aagataatgg ttcagctcga tttcctcgct gaccatcgat ttgcctttga
     5341 ccatcagctt gccgtttttg gctgtgatga tgtcgtggat aatttggcag gcttcggtcg
     5401 gggtttctgc ccagtgcact ttcacgccca acttagtcag gttttcttcc agctgctcca
     5461 gcagggctgg caatttagac aatgagcgct gacggacgtg ttcgcacaaa tcgcgcaggc
     5521 tttgcagctc ttcttcgtcg gtcaaaacgg ctttgcgttt ggtcatcagc atatccatcg
     5581 cggtacgcag gcttttgcgc aaaggcttgt cttgaaggga aattgcggcg ttttgcttga
     5641 aagtttccgg cttcatgtga aatttgatgg tttgcgtagt catgcgtttt cctccaaatc
     5701 ggcaggggaa atgtggtcgg gcaggatggc gaggatgacc aaatcgcgcg ggccgtgcgc
     5761 gccgtaagca agcgtcagtt ggatgtctgc ggttttggac gggccggaaa tcaggaatac
     5821 attggtcggc ataccgtttt ccaccagttt ttcgccttcg acggcattat gaaactcgtt
     5881 gtacatcttg gacgtatcga acaggcagaa atgcacggga ggaacgaggc ttaaagtacg
     5941 cggctcttcg gggctggaaa acagcatcag cgtgccggtg cgggcgatgc cgcattgcgc
     6001 gccgctgaag cccgcatcga tgttcgtgaa aaactcggtt ttccaagtat cgatttcgcg
     6061 ctcgaaggca atcggttcga tattgctgtc cgccaatgcg gcacgggcaa tttgtccgtg
     6121 ttcggtcgcc aagggcagca ggatgttttt caaacccttg ccttctgccg cttcgcggaa
     6181 aacctgcatc caattgcttt tcgtcaccca ataaatttcg gttttgaccg cacgcatagc
     6241 ggcagcccaa tgtttcagac gctcaacttc gctgccccaa gaaacaccca tttcacggta
     6301 ataatcaaaa accgcaggtt cttccatcgg caatgcgtcg gcttttttca gttttgccaa
     6361 aatattttca cgcgcgctca tgctttgcct ccggtgcgtt ccaacaagaa ggatgcgata
     6421 tgtttcggac gcggcatatc cggctcgtcc ttggcgattt tgccgccgat gttcatcata
     6481 cagccgcagt ccgcgctgat gatttcggtt gcgccggttt ctttcagcgc ggcgactttg
     6541 tctgttacca ttgcgccgga aatatcggct tgtttgacgg agaatgtgcc gccgaagccg
     6601 caacattcgc tttcgtggtc gtggacgatg cgttcgacgt tttccatacc gtcaatcagt
     6661 tgccagcctg aaagatggac attcatttcg cggcgggcgg cgcaggaagt gtgaacggcg
     6721 actttgagcg gttcgccctt gtcttcgggt ttgaaaccga tggcaagcag gaaatgggta
     6781 aactcgatga tgcggccggc gcaatccaca gccctttcct cgtactcgct gcctttaaac
     6841 agcgtcggcc agtggtgttt catcatgccg ccgcacgagc cggacggcac gacgatcggc
     6901 cagttttcag ggaaaaggtc gagttgtgct ttggcgacat cgaaggcttc ggtcggatgg
     6961 ccggatgaat aggcaggctg gccgcagcag ctttgcgcca tcgggaaatg gacgcgtatg
     7021 ccctgctgct cgattagggt aatggcatcc atgcctgctt cgggcataaa aagatcaagg
     7081 acgcaagtgc cgaaaaaata gacatcggtc ggattgctgt cgtatcggat gatttttggg
     7141 ggaatggttg cgctcatgtt ttttgataac gggaaacccg tttttctctg tagaaaggta
     7201 agcgtttact ttaagtaatt gactgttgcg ggtcaagtct aattttaaaa aataatccgg
     7261 tttttcttac aaactgcccc cataacgctt actgtacctt aatctgatgg ttttcgataa
     7321 taattatcat tacaatgcaa tgccggttcg tttgcttgtg aacattcaag atgccgactc
     7381 tgacggcatt cagacagcat ctgaaaacaa taacggcaca aggatggggt acaatcgccc
     7441 tatggaaaaa aacaccctat ccgcccgcgc accatcccct tggctgccgc ttctgctggc
     7501 aattgccatt tttatgcaga tgttggatgc gaccatttta aataccgcac tgcctgaaat
     7561 tgccgccgac ctgaatgagt cgcctctgga tatgcaactg gcagttattt cctacacgct
     7621 gacggttgcc ctgctgattc ctttgagcgg ttatttggcg gacaggttcg gaacgaaaaa
     7681 agtctttttc ggttcgattg ccgtttttat gctcggatcg gcattgtgcg ccgcatcggg
     7741 ttcgctgttt gaattgacgc tttcccgtgt cgttcagggc atcggcggtt cgatgctggt
     7801 tccgataccg cgtctgacca tcttgcgtgt gtacgacaag tccaagctgc tcaatgccat
     7861 caattatgcg gttatgcccg cattaatcgg gccggtttta gggcctttgg cgggcggtta
     7921 tttggtcgaa tacgcttcgt ggcactggat tttcctgctc aacctgccca tcggtctgct
     7981 gggtttcata ttgggacgca acatcatgcc cgatattaaa ggcagtaata tctctttaga
     8041 cttcaaaggt tatctgattt tttctgccgc cgcgtgcctc ttgttacttt cggcagaaag
     8101 cctgtcgcac gcgctgcctc cgtattttgc actgttgccg ctgtgcggcg gactgctgtt
     8161 tgcacgccgt tatttccgac atatgaaaac cgcgtccaaa ccgatttatt ccgccgacct
     8221 gtttctgata cgcactttcc gtctgggact ggcgggcaat ctgttcagcc gtctcggcat
     8281 cagctcgatt ccttttctga tgcccctgat gtttcaaatc gctttcggct tcggcgcaag
     8341 cctgtcgggt tggctggtcg cacccgtcgc cctgtcttcg ctgctggtca aaccgctgat
     8401 tgcaccgctc atgaaacgtt tcggctaccg cacggtactg ctttggaaca ccaagctgct
     8461 tgccgccttc atcatgctgc tcgccctgcc tgacggaaac tcgccgctgt ggatttgggt
     8521 tttcctctcg ctggcgatcg gcgcgtgcaa ctccctacag ttttctgcca tgaacacact
     8581 gaccctcgcc gatttgcgcc cgcaacaaac aggcagcggc aacagcctga tggcggtcaa
     8641 ccaacagctt gccatcagca tgggcattgt tgccggcgca ttaatcctta aaaactggac
     8701 atttctgata ccggcttctt caggtctgca ttccgccttc cgtatgaccc tgctcagcat
     8761 cggcggcatc acccttgcat catcgctggt tttcaaacgg ctgcacgttt cagacggcac
     8821 caacctgaca cggaacacac cgtcctgaag cggtccacac gcaaaacttt tacccgtttc
     8881 aacgtttgga ttatgatacc gcacttccat gcgcgccaac cccaaaacac aggcaatgcc
     8941 gtctgaaacc atatccctga tgaaaacacg cagcctaatt tcccttttat gcctccttct
     9001 ctgttcatgt tcttcatggt tgcccccact ggaagaacgg acggaaagcc gtcatttcaa
     9061 tacttccaaa cccgtccgcc tggacaacat cctgcaaatc cggcacaccc ctcataccaa
     9121 cgggctatcc gatatctatc tgttgaacga cccccacgaa gcctttgccg cccgcgccgc
     9181 ccttatcgaa tctgccgaac acagcctcga tttgcaatac tacatctggc gcaacgacat
     9241 ttccggcagg ctgctgttca acctcgtgta ccttgccgca gaacgcggtg tgcgcgtacg
     9301 cctgctgttg gacgacaaca acacgcgcgg attggacgac ctcctgcttg ccctcgacag
     9361 ccatcccaat atcgaagtgc gcctgttcaa ccccttcgtc ttacgaaaat ggcgcgcact
     9421 cggctacctg accgacttcc cccgcctcaa ccgccgcatg cacaacaaat cctttaccgc
     9481 cgacaaccgc gccaccatac tcggcggacg caatatcggc gacgaatact tcaaagtcgg
     9541 tgaggacacc gttttcgccg acctagacat cctcgccacc ggcagcgtcg tcggcgaagt
     9601 atcgcacgac ttcgaccgct actgggcaag ccattccgcc cacaacgcca cgcgcatcat
     9661 ccgcagcggc aacatcggca agggtcttca agcactcgga tacaacgacg aaacgtccag
     9721 acacgcgctc ctgcgctacc gcgaaaccgt cgaacagtcg cccctctacc aaaaaataca
     9781 ggcaggacgc atcgactggc agagcgtcca aacccgcctc atcagcgacg accctgcaaa
     9841 aggactcgac cgcgaccgcc gcaaaccgcc gattgccggg cggctgcaag acgcgctcaa
     9901 acagcccgaa aaaagcgtct atctggtttc accctatttc gtccccacaa aatccggcac
     9961 agacgcactg gcaaaactgg tgcaggacgg catagacgtt accgtcctga ccaactcgct
    10021 acaggcgacc gacgttgccg ccgtccattc cggctatgtc aaataccgaa aaccgctgct
    10081 caaagccggc atcaaactct acgagctgca acccaaccat gccgtccctg ccacaaaaga
    10141 caaaggcctg accggcagct ctgtaaccag cctgcacgcc aaaaccttca ttgtggacgg
    10201 caaacgcatc ttcatcggtt cgttcaacct cgacccccgt tccgcgcgtc tcaacaccga
    10261 aatgggcgtt gttatcgaaa gccccaaaat cgcagaacag atggagcgca cccttgccga
    10321 taccaccccc gaatacgcct accgcgttac cctcgacagg cacaaccgcc tgcaatggca
    10381 cgatcccgcc ccccgaaaaa cctacccgaa cgaacccgaa gccaaacttt ggaaacgcat
    10441 cgccgcaaaa atcctatccc tgctgcccat agaaagttta ttatagaaat atagcggatt
    10501 aacaaaaacc agtacg
//
LOCUS       DAWHRS010000064        10479 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWHRS010000064 DAWHRS010000000
VERSION     DAWHRS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.064"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(182..394)
                     /locus_tag="WIB82_001143"
     CDS             complement(182..394)
                     /locus_tag="WIB82_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3842447.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAKPD
                     TETRRTDMSLEKWRQFLDWTEKTNAV"
     gene            complement(406..687)
                     /locus_tag="WIB82_001144"
     CDS             complement(406..687)
                     /locus_tag="WIB82_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842448.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(725..988)
                     /locus_tag="WIB82_001145"
     CDS             complement(725..988)
                     /locus_tag="WIB82_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842449.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1008..1580)
                     /locus_tag="WIB82_001146"
     CDS             complement(1008..1580)
                     /locus_tag="WIB82_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEZ3842450.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVL
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1559..1870
                     /locus_tag="WIB82_001147"
     CDS             1559..1870
                     /locus_tag="WIB82_001147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842451.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1892..2767)
                     /locus_tag="WIB82_001148"
     CDS             complement(1892..2767)
                     /locus_tag="WIB82_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ3842452.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2812..3102)
                     /locus_tag="WIB82_001149"
     CDS             complement(2812..3102)
                     /locus_tag="WIB82_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ3842453.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3114..3359)
                     /locus_tag="WIB82_001150"
     CDS             complement(3114..3359)
                     /locus_tag="WIB82_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ3842454.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAKNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGHEIAKKLGLK"
     gene            complement(3352..3576)
                     /locus_tag="WIB82_001151"
     CDS             complement(3352..3576)
                     /locus_tag="WIB82_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ3842455.1"
                     /translation="MEKPLNFKPIPYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            3848..4219
                     /locus_tag="WIB82_001152"
     CDS             3848..4219
                     /locus_tag="WIB82_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3842456.1"
                     /translation="MNNSSFFGNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGIS
                     QPKAELFFQFEKVGIDVQYVMHGRRGETAVMPSETLNAEEQELLVLFREAAAADREMI
                     LMVARRAEKKAQTALGKVSNG"
     gene            4221..4844
                     /locus_tag="WIB82_001153"
     CDS             4221..4844
                     /locus_tag="WIB82_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842457.1"
                     /translation="MDQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLWARS
                     AKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSIYDL
                     LGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKKFVM
                     PCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            4837..5031
                     /locus_tag="WIB82_001154"
     CDS             4837..5031
                     /locus_tag="WIB82_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3842458.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            5055..5618
                     /locus_tag="WIB82_001155"
     CDS             5055..5618
                     /locus_tag="WIB82_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842459.1"
                     /translation="MKILALLIAATCALSACGSQSEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSAANKGLNDQKTGLTLPEHVVPIDNAEGKNLLHDFSDGLTILTVDTDKADKIT
                     AVRVVWNTDAMPQKAEKLSKAAAALIAATAPEDRTMLRDTGDQIEMAIDSHNAQKEPT
                     REWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            5649..6596
                     /locus_tag="WIB82_001156"
     CDS             5649..6596
                     /locus_tag="WIB82_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842460.1"
                     /translation="MMIEKSISIVDGKEYSVFAVSHEFRYTFDEPILVADLISSLKAY
                     ETLTSSYLPAILNQLFDVKIQKIKVAVSEIERGSFLEKLIFNLFFKDEDAYNEFCLKI
                     RKFLGTENQDGSINMSKIIMFAMTTLLGVGAGYLLFKNPPQEKQAITNNIVTVINADS
                     SVALDGEHLVSVVKEVTGSSKQKTAENVAKVYAPASKNNGSITLGTDDVRIEPVAQQT
                     VATLPKDVDLRDTPLTEDYTDIDVQIRATDRDKNSGWYAVIDQIVPSRVRLELPEDID
                     LNRLANNATIRANVTVEFDLKQNGSRKPKKIILTSLSTD"
     gene            6681..7184
                     /locus_tag="WIB82_001157"
     CDS             6681..7184
                     /locus_tag="WIB82_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ3842461.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRIAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGVIGAVSLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            7198..7371
                     /locus_tag="WIB82_001158"
     CDS             7198..7371
                     /locus_tag="WIB82_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842462.1"
                     /translation="MSKKSLIALMTAAMQPNFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            7296..7628
                     /locus_tag="WIB82_001159"
                     /pseudo
     CDS             7296..7628
                     /locus_tag="WIB82_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7625..8035
                     /locus_tag="WIB82_001160"
     CDS             7625..8035
                     /locus_tag="WIB82_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ3842463.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEVRQKERNSSQG"
     gene            8068..8601
                     /locus_tag="WIB82_001161"
     CDS             8068..8601
                     /locus_tag="WIB82_001161"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ3842464.1"
                     /translation="MSFYYQLAVQSAEDKYADLKRHIHDIYRRHKGRYGYRRIAAAIR
                     HAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNILRRCFHAEKPNEKWV
                     TDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEILKGAPEKPGSSEKPI
                     LHSDQGWQYQMFFIKSS"
     gene            8625..8735
                     /locus_tag="WIB82_001162"
     CDS             8625..8735
                     /locus_tag="WIB82_001162"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ3842465.1"
                     /translation="MSRKGNCLDNAAMESFFGTLKSECFHTCKYDSVTES"
     gene            8742..8843
                     /locus_tag="WIB82_001163"
     CDS             8742..8843
                     /locus_tag="WIB82_001163"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ3842466.1"
                     /translation="MHEYIRYYNNDRIKLKLKGLSPVQYRTQSLKAA"
     gene            complement(9161..9523)
                     /gene="ssrA"
                     /locus_tag="WIB82_001164"
     tmRNA           complement(9161..9523)
                     /gene="ssrA"
                     /locus_tag="WIB82_001164"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(9586..10191)
                     /locus_tag="WIB82_001165"
     CDS             complement(9586..10191)
                     /locus_tag="WIB82_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ3842467.1"
                     /translation="MNTDFQNILKQLGKQAKKEAAEKQEAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPSDKTPIKVRPKDNRADEENYFYIGSTYNEPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2549 a         2447 c         2657 g         2826 t
ORIGIN      
        1 aacctgtgtt tgggtttcgg ctgtcgggag ggaaaggaat tttgcaaagg tctcagagtg
       61 agtttatttt ggggcggcgg caggtcgggg gcaagcggcg tggggcttgg ttgtggtttt
      121 taggtttggg ggtgggtaaa aaatgccgta cccggcgggc aggcttcgca ctctgaaggt
      181 ttcagacggc gtttgttttt tctgtccaat cgaggaactg ccgccatttt tccagcgaca
      241 tatcggtccg ccgcgtttcg gtatcgggtt tggcttccca gcggcctacc tgtatgtagt
      301 gcttcacccc gacgatttgc gccaactcgg cctgtgtcag tttgcagcgg ttgcgcaggg
      361 tgcgaaggtt gtaaggcgtg tagccgagtt ccatgtcgtt gcggtttatt ttgtttcgca
      421 tatttttttg actgcccggc ggcaggtttc ggtaaggacg gcggcaaatc gggcttcgtc
      481 tgccggtttt ccgtcgaaaa aaataaaatc gtaaagggtg atgccgtttt cggaatggcg
      541 gtaatgcccg cccgggcatt cgctgtctgt ctgcctgtgg attttggcaa tcaggcgggg
      601 atagcggcaa tgggtaatga aacggcttgc gccgtcttgc ccgataatcc attcggggta
      661 gcggttgaat agtgcggctc tgttcatttt gttcggggga taaagcccct cgcggggctt
      721 gtggtcaggc aaatttgaat atcagtgcca acacggcggc gatggcggta accagcccgg
      781 tggccgccat catgggatac catcgggatt cttgggctat ttttacggat tcggcgttga
      841 ttttgtgcgc gtctgcaatg attttggcga tttcggcatc tacttttctc agactggagt
      901 gtttcatttc ttgttcgatt atgttcatcg tacttccttt cgtttttggc ggttgccgcc
      961 gcttgtcgga tggtaggatg tctgccatgt gtatatattg atacctttta ggttttattg
     1021 caagtgtttt gggcggcggc ttcgtatgct tggcggtggc ggcggctgta ccattcggcg
     1081 agttcgcgcc gctcttggag gcggtagctg tcggcggtca ggtagtggtg caggcttgag
     1141 aagccggcgc gttggagggc ggcgcggctg atgtggccga gggcgaggtc tgttgtgagg
     1201 gtgtcttttc cggcggtcag gctgatgttg gtgaagaggt ggcggaatcc gtgcattgtg
     1261 tgtttggatt tgccgggggt gctgccgtca tagcccagtc gtcggatggc gttgtgggcg
     1321 aatttgatgc tgatgtggtc gggatggggg gcgggtttgc gccgtgggcg gatgccgggg
     1381 aaaaggtgga tgttgtcgcc ggtctgtgtg tgcagctctc ggagtatttc taccgcccag
     1441 tccgacagta ggacggtaaa ggggtgtttg gtcttcatgt cggcggcggg gatgtgccat
     1501 aaccgggcgg tgaggtcgat gtcttgccag cgggcggaaa gcagtgcgga cggacgggat
     1561 gacttccccg gtttccccat caatccgcgc cgcctcgatt tttccgttgg cgcgcagcat
     1621 ttccaccgcc tgccgcaccg acatcaaccg cttgccgttg cgcctcatcg cctccaccaa
     1681 aaccgccgaa atcaatttgg cttcttccgg tttaagctcc gtcttgcccg catcgctgcg
     1741 ccgtttgcgc gtcggcttga cgctgaccgc ctccagcttg cggtatagcg tggcaaggct
     1801 gatgcccaat tcctgcgcct gctgcttaag atatgcagag cgtgcgccgc gttccattgc
     1861 ttccgcctga ttctcgactg ccttaagacg ctcaatcatt gccggattca tcgccttctc
     1921 ccgtttcacc gcccaaccat tccggcacat tgtctgtcgg tgcttcagtc ggcagggcat
     1981 agctttcgcg cagttgctcg cagtccaaaa taatttgatt gagcgtgccg accatctttg
     2041 cctgatggct gatcccgtgt gcctcactgt gcgcattaag ttggtcgaac aaatctttca
     2101 gacggctcac ttgactgcgg ataccgacct caaggcttgt taactgcatc gccaactcgc
     2161 tgcctacgtc ttccgccttc ggctctctga caacggtttg cttcttagcc agcttctcgg
     2221 ccagctcatc aattttggct gttttggttt tcatcacttc gtctttggcg gcgaggtttt
     2281 cgcggctttc gcgcagggcg acgcgcagct cgcgcaccgt cattcggtcc acatcgtcaa
     2341 aggtcatgcc gttgacttct tccccttcgg ccaaacccac cagcgtaacg tcttcttcga
     2401 ccagcagctc aagcagcttc gacttgccca aatccatcag cttcggcgcg gctttctgca
     2461 tttgcggggt cgcaaagcgg cgagtggctg acatcagacg tgatgtttct gcgataccca
     2521 gcccaaattg gcttttcaca atttccataa accgtccgtg ttccgtatgc tcttttaaaa
     2581 taatcagcgc acgtcccagt tcgaacatgc cttccatcgt ctgccgtact gcttgacgac
     2641 cgcgttcaac ccaacgctct tcgctataag tctcgccgtt accccactgc tccatcacca
     2701 gcacactgcg catcgctgcc tgattgctta ctttgtcggt tgcgataatt tcaatttctg
     2761 tattcatttt ttatctccaa agtttccgac gtcggaaact ttcaaaatcc gttaatccac
     2821 atcgacccgc ttgccgattt cggcaatctt gctttgcagc cgttcatgct gctgcctgaa
     2881 ccgctctgcg atttgcaggg ttttgatgcc gtaggcgtag ttgccgtttt caagtttgat
     2941 gaccaatccc gaggcaacca aatcatcaat atccctgctg acttgcgatg gcgtcagccc
     3001 cagtccgacc gataaatcct tattgctcag accgataatc ggatgctcgt caagcgcgat
     3061 aaagaccctc aatagccgtt gtaccctttt actttctgcc atccgcatcc tccttatttc
     3121 agtcccagct tcttggcaat ttcgtgcccc ttgccgtaat tgcctttgcg ctgtccgccg
     3181 atcaccagat acacatcgcg cggcttaaag ccgttctctc ttgcccactg cgccagcgtc
     3241 tggccgtttt tggcaaaatt ttctttcaat tcgtctgccg taagcatttc tttggcctgt
     3301 acgcgggaaa atttcagtat ccgtccgtac ggttggttaa tacactcatg attaatcatc
     3361 gcttttctcc ttcatcccca gcaaaaccgc cgcttcgtgg cttttgccaa aattgccttt
     3421 cagcttgccg cgcaagaggt gctccaccgt ggtgcgctcc agattgaaat atttcgccca
     3481 atgcgccttg cacaccccgt tgcgcttaaa ccacccggcc gcgctctcgc gcgtttgcgg
     3541 atagggaata ggcttgaaat tcaaaggttt ttccatatta tttatgcctt tcgtgtgata
     3601 taatgtgttt gtttttaggt ctctttgcac gataaacatc cggtctgttg tgcagcagca
     3661 gggtcttggc ttgttttaaa gtaaacaagc tttttctcgt agctgggtgt atgtgatcca
     3721 tcatgagctg tcgcgcatga gccttcaaag tgttcatttt ttcgtccttt ctcgtgatga
     3781 tttagggtgt ttgtgtttcg atgtggaaat tataggaaga attcttccta ttttgcaagg
     3841 aatatttatg aataactctt ccttttttgg caaccgattg aaagaagaaa gaaaaaaatt
     3901 aaaaatgact caagctgaaa tcgctgaaaa atgtggggtt tcaggaagaa tgtgggggga
     3961 ttatgaacgt ggcatcagcc agccaaaagc ggaacttttc ttccaatttg aaaaggtggg
     4021 tatagacgtt caatacgtca tgcacggcag acgcggcgaa acagcggtca tgccgtctga
     4081 aaccctgaac gccgaagaac aagaactgct ggtcttgttc cgcgaggcgg cagctgccga
     4141 ccgtgaaatg attctgatgg ttgcgcgcag ggcagagaaa aaagcccaaa ctgcgcttgg
     4201 taaagtgagt aatggataaa atggatcaat tcgaattgtg ccaaaaagaa catgtcaatc
     4261 catttgcctt gtcaaagcaa tatctattgg ttgtaacatt cgtcaaatcc agcagtaaaa
     4321 attttcaggc agcattactt tgggcaagaa gtgccaaatt atttgagaat cttgagattg
     4381 gaaaagaaac catctattgc tgcgctttcg ataaaacagc agaacaggct gggatggccg
     4441 gggtattttt gaattatatt gaaaattgga atggcaaaca gatttacatc aatggccgaa
     4501 tccatagtgg cagtatttat gatttgttag gggttttaga ctgctatcaa aaatcacagt
     4561 cctgtcccaa ccctaaaagc cactgttgct ttgtttcaga cgacattttt ctatggcatg
     4621 gatcaagacc aacgtttgaa atcagtcttg atctaactgg aaagaaaaaa gaaacatcct
     4681 ctgcaaagaa atttgtgatg ccttgtatta atttccgtca ccataggatt gaaaaagaaa
     4741 cctacttagg aaattggaat gaacaaattg ccgcattggc agtaaaacaa aatatagatt
     4801 ggtgtccaag ttttgatatt gagaatttta gacagtatga ataattacta tctatatagg
     4861 aattgcagca gcgatgtgtt atgggtcaaa cgtatccaac gccaaatcga cggcagccta
     4921 ctcttgattt ctgacaattc aacctatcca cccatgccct tggcactggc ggaacacccc
     4981 gatattcaaa tcatcgggca ggtagtgcag gtatcaaaag acttgaacta gacacaatca
     5041 aaaagggaaa tagaatgaaa atactcgctt tattaattgc cgctacctgt gctttatctg
     5101 cgtgtggcag ccaatctgaa gaacaaccgg catctgcaca accccaagag caggcacaat
     5161 ccgaattaaa aaccatgccg gtaagctata ccgactatca atcagcagcc aataaagggc
     5221 tgaatgacca aaaaaccggt ctgacccttc ctgaacatgt tgtccctatc gacaatgcgg
     5281 aaggaaagaa tctgctgcat gacttttcag acggcctcac aatcttaacc gttgataccg
     5341 ataaagccga caaaattact gctgtccgag tagtctggaa tacagatgca atgcctcaaa
     5401 aagcggaaaa actgtccaaa gctgccgcag ccttgattgc ggcaaccgct ccggaagacc
     5461 gcacaatgct gcgtgatacc ggcgaccaaa tcgaaatggc gattgacagc cataatgcgc
     5521 aaaaagagcc aacccgagaa tgggcgcgtg gtgggattgc ttataaagtc actgttacca
     5581 atttaccgag cgtggttttg acggcaaaag ctgagtaaat ctattaagta gaaaaaatag
     5641 aaagggaaat gatgattgag aaaagtattt ctattgtaga tggaaaggaa tactccgttt
     5701 ttgctgtatc acacgagttt cgttatacct ttgatgagcc tattttagtc gctgacttga
     5761 ttagttctct aaaagcttat gaaacactga caagtagtta tcttccagca attttgaatc
     5821 agctgtttga tgtcaaaatc caaaaaatca aagtagctgt atctgaaatt gaaagaggat
     5881 ctttccttga aaaactgatt ttcaatttat tcttcaaaga tgaagatgct tataatgaat
     5941 tttgtcttaa aatacgaaaa tttctaggaa cagaaaatca ggacggaagt attaatatgt
     6001 ccaaaatcat tatgtttgca atgactacac ttttaggggt aggtgctggt tatctcttgt
     6061 ttaaaaaccc gccacaagag aagcaggcaa taaccaacaa catcgttacc gtcattaatg
     6121 ctgatagttc tgtcgcactg gatggtgaac atttggtttc agtggtaaaa gaagtaacag
     6181 gaagcagcaa gcaaaaaact gcagaaaatg tggcaaaagt atatgctcca gcaagtaaaa
     6241 ataatggcag tattaccctt gggacagatg atgttcggat tgaacctgtt gcacaacaaa
     6301 ctgtagcaac tttgcctaaa gatgtggact tacgtgatac gccattgact gaagattaca
     6361 ccgatattga tgtgcaaatt cgtgctactg accgtgataa aaattcaggg tggtatgcag
     6421 tcatagacca aattgttcca tcacgtgttc gattagaact gcctgaagat attgatttga
     6481 ataggctggc taacaatgct actatccgtg caaatgtaac agttgagttt gacttaaagc
     6541 aaaatggctc tcgtaagcct aaaaaaatca tcctcacatc tctctctact gattaagttt
     6601 taacccgtat taaaggctta gtcagacggc ctttcctaca atccctgtat tgatttttaa
     6661 ttcaatacag ggatttttcc atgtcagaca agttcaacca attcatcaac cgcgtcctct
     6721 ctcacgaggg tggttacgcc aaccatccca aagaccccgg cggcgaaacc aattggggca
     6781 tcactaagcg catcgcacag gcaaacggct acaacggctc catgcgtgcc atgacgcgtg
     6841 aacaggcaat cagcatttac cgtaaagcgt tttgggagcg ttaccgcgcc gaccaaatgc
     6901 cggaagcggt cgcgttccaa ttttttgatg cctgcgtcaa ccacggttac ggcaatgccg
     6961 cccgtatgct gcaacgcgcc gcaggcgtac cggacgacgg cgttatcgga gcagtcagcc
     7021 tcaaagccat caattccctt cccgaaaacg accttttatt gcggttcaac gccgagcgtc
     7081 tggtctttta taccaagctc ggtacgttca cctctttcgg caagggctgg gtacgccgtg
     7141 tggcgcaaaa cctgattcac gcgtctgcag ataacactga ttaaagggag ataaaccatg
     7201 tcaaaaaagt cactcatcgc cctaatgacc gcagccatgc agcccaattt cagccacagc
     7261 gacctaggca ttcgctacgc catgccgact cagggatgtt ggacgcaagc ccaccgcaag
     7321 agcggggtag ccgccgcgaa acgcgcagcc aaaaaaaagc gtcacaaata accgcctttt
     7381 tccgatggct gggcggcttg gtctctaatc cggccacagg aaaaatcagc cataccaaac
     7441 tatgggcaaa cgtcgccgca gccgccatga cttggaagtt cgtgcaggcg gcggacgcgc
     7501 ccgaatggct ctagtgggct tatggcgcat tggtcggcgg gtatgcatta atcaaacgcg
     7561 gcatcgcggc gattccgcag ttggcagaaa tcaaaaaatc cgcaaatcag gaaggggggc
     7621 ggcaatgatt gaatttgtcc gagccaaaaa acggctgctt tgggcatttg tgcttttgct
     7681 tgtgtggacg tgcggttacc gatacgccgc cgacaaggcc gaagcgaaac aaaccgccct
     7741 gattgccacc tatcggcatt cttctatggt tgcggcggaa caatatgcct tgcagcttaa
     7801 aaaagcgcag gacgaaaggc agcggtggta cgacttttcc caaaaacaag gaagaaagcc
     7861 cgtgaaaaaa cagtatccgc cgcaaacgaa aaaagccggc tatctgaaaa ccaaggaaga
     7921 actgcttgcg gaattggctt gccttaaagc ggaaatggtt gccctaaaaa agcccgatgc
     7981 cttaatccat gggaaagaag tgcggcagaa agaacgcaac tcgtcgcagg gttaaggcaa
     8041 tgccatccgt tgaactgctg ttggagattg tccttctatt accaattggc cgtccaatcg
     8101 gcagaagaca aatatgccga tttgaaacgg catatccatg atatttatcg acgacataag
     8161 ggaagatacg gctaccggag gattgcggca gccatccgtc acgcaggaac accggtcaat
     8221 cacaagaaag tcagccgtct gatggcgaag acggggctga aggcagtgat acggcggcgc
     8281 aaataccgct cgttcaaagg agaagtcggc aaaattgcgc cgaatatcct gcgacgctgt
     8341 ttccatgcag aaaagccgaa tgagaaatgg gtaacggacg ttaccgagtt caatgtaggc
     8401 ggagaaaaga tatacctttc tccgattatg gatttgttta acggggaaat cgtcagttac
     8461 cgtattcaaa tccgcccgac tttcgatttg gccggcgaga tactgaaagg tgcgccggag
     8521 aaaccgggat cgtctgaaaa gccgatactg cattcggatc aaggttggca atatcagatg
     8581 ttttttatca aaagcagttg aaaggcaacg gtctggttca gagtatgtcc cgcaagggaa
     8641 actgcttgga caatgcggca atggaaagtt tcttcggaac gttgaaatcg gaatgtttcc
     8701 atacgtgcaa atatgattcc gttaccgaat cgtaagcggc actgcacgaa tatatccgtt
     8761 actacaacaa cgatagaatc aagttgaaat taaaaggact gagccctgtt cagtacagaa
     8821 ctcagtccct gaaagccgct tgattaaact gtccgacttt ttggggtcag ttcggcttcg
     8881 gcattttttt atccgttttg ggggtaactt gtttggaaag ctgcaagctt ataaataaag
     8941 gattacattt aagttttggg taaccttttt aaaaaaatgc gtgatgactt ttgcattttt
     9001 aaggcgtttt ttggggtaat tcgtgaaaag ttaccccaaa agttacccca taaatggcga
     9061 aaactcaagc atacgccagc atcctgcaac acaaaaaagc cttgaaactg ttgaagttca
     9121 aggctttttt gtgttgcagg atgctgctga aaatagggta tggtggaggc ggggggaatc
     9181 gaacccccgt ccgaaagtcc tctacaaagc gttctacata cttagttgtg tctatttgaa
     9241 aatcttattt ccatcatgcc gaccaacagg ccttttggaa accagttacc ttaagtctta
     9301 tttcctgcca agtaacccgg taggaaacca gtcaatgtaa gatgacgttg cggtggcttt
     9361 cgccacacag cccattgacc gactgctgca acggctagcc ttaagcggct aaagcgtaag
     9421 tttcgtcgtt tgcgactatt tgaattcagt gttttacggg aatctgagac cccggtatgc
     9481 ccgcatctgc ttcgcaaccc ccgtcgaaac caaggtcgcc cccagaaatg gtttgcaaat
     9541 tatacggata ttgtgcggtg ctgccaagtc tgtcggagaa atttgtcagt cttgctgcct
     9601 taatttgcgt ttgagcagga tgcggacgca gccgtcgttg ccttcccggg gttcggcgta
     9661 ggcgagtacg tcggggtgtt gcatcagcca gtttcgggtc atatttttca gaacgggttt
     9721 gtagcctttg gaacctaatc cgctgccgtg gatgatttcg ccgcatacgc cgcgtttttg
     9781 ggtgaatgcg atgaattcgt tgaggacttt ttgggcttct tcctgtgtgt agccgtgcag
     9841 gtcgacatcg gtaacgacgg gatagtatcc gtttttcagg cgttggatgt cgttttttcc
     9901 ctgtccgttt ttgctgaagc tggcgggcgg ctcgttgtat gtgctgccga tgtagaagta
     9961 gttttcttcg tcggcgcggt tgtctttggg acggactttg atgggggttt tgtcggacgg
    10021 tgcataatat tgttgccggt tttttaacgg ggagacttgt ccgactgctt gtgaaaaatc
    10081 gaaatcctgt tcttgttttt gcttgttttt ttcggcctcc tgtttttctg ccgcttcttt
    10141 tttggcctgt ttgcccagtt gtttgaggat gttttggaag tcggtattca tattttttcc
    10201 tgttatttgt ccgatggctg tttcgggcgg ggttttaatt tgccggaatg tttgccaagc
    10261 gggggaggat gattttgttg ccagcgtatg ttttttgaaa gtgtgattgt atatcaaaaa
    10321 gaaatgcggc aaccgtcggc agtgttgatt gccggaaatg cgggccggtc gaaccgatat
    10381 gcccgaacgc ctgataaagt tttaaaaacc tgccttgcga agcaggttaa tggttttgcc
    10441 aatcttgaat tgccggaaac gcgaaacacg gaaatctga
//
LOCUS       DAWHRS010000065        10371 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWHRS010000065 DAWHRS010000000
VERSION     DAWHRS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10371
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.065"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WIB82_001166"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WIB82_001166"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ3842468.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVNADYAVKVPDGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1457..1846)
                     /locus_tag="WIB82_001167"
     CDS             complement(1457..1846)
                     /locus_tag="WIB82_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ3842469.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFATYDKNKLKQNEYFKITLSEVSPDHFTLQAAPDPMTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2072..3250
                     /locus_tag="WIB82_001168"
     CDS             2072..3250
                     /locus_tag="WIB82_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ3842470.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3316..5250
                     /locus_tag="WIB82_001169"
     CDS             3316..5250
                     /locus_tag="WIB82_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ3842471.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5667..6449)
                     /locus_tag="WIB82_001170"
     CDS             complement(5667..6449)
                     /locus_tag="WIB82_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ3842472.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6588..8777
                     /locus_tag="WIB82_001171"
     CDS             6588..8777
                     /locus_tag="WIB82_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ3842473.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGIV
                     WETDIAPDMDTARILSVQTAFMEEKPLPQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSYHGQASHSEFVLNADQQKASDEIQTALGRFRPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESVSRQDLHRAVSLWVQVLQQNRDSKIRWSVDVDPQEA"
     gene            complement(8823..9897)
                     /locus_tag="WIB82_001172"
                     /pseudo
     CDS             complement(8823..9897)
                     /locus_tag="WIB82_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(9945..>10371)
                     /locus_tag="WIB82_001173"
     CDS             complement(9945..>10371)
                     /locus_tag="WIB82_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ3842474.1"
                     /translation="THFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLVG
                     IAPHPRESGETKFKSRCFGGRSAVLKALYMATVAATRFEPLIRDFYQRLLSEGKPYKV
                     AVTVCMRKLLTISNARMRDYFAENDTAENDTAENGIRTA"
BASE COUNT         2397 a         2681 c         3023 g         2270 t
ORIGIN      
        1 gacgtgttgc gataagggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtactgcca
      121 cgtcaaatca gtggtgatgg ccgcagccgc attctttttt catatcgatt accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagcccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgccggtttt ttcctgaatc actttggcag cgtcttcttt ggcggcgttg acaaccaaat
      541 ccgcgccggt ttctttggca aacgccagtt tgtcgtcgtt gatgtcgatg gcgacaacgt
      601 gcgcgccgaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtca
      721 cgccggcgca agtgatgctg gacgcttggg cggggtcgag accgtcgggg actttgaccg
      781 cataatccgc attgacgatg cagtgggtcg ccataccgcc gtcggcggtg tagcccgcgt
      841 tcaatacaga gcggcacagg gtttcgcggc cggtattgca gtattcgcaa gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca gcttggaaag
     1321 gcgcggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 cggcaaaccc gtaaagatac cgtctgaagc tgtgtccgga cggcatcttt acgggtttgc
     1441 gggcttcggc ggaggattag tcgaagccgg ggcaggattg gtttgtaccg gaagcggcaa
     1501 tggtaccgcc gtcgttgagc gtaacgacgc aggtttcgcc gtcgttggtc atcggatcgg
     1561 gggctgcctg aagggtgaag tggtcggggc tgacttcgct taaggtaatc ttgaaatatt
     1621 cgttttgttt cagtttgttt ttgtcgtagg ttgcaaacgt ccctttttgg cggtagtaac
     1681 gttccatggt ctgcgcgttg tgcagcaggg tcgtcctgac ttccgacagg cggacgcgcc
     1741 ggatgtaggt tttataggaa gggtaggtga tgagcgtcag gatgccgagg atggcgacgg
     1801 caatcatcag ctcgagcagc gtaaagcctt tttgaacgtt tttcatagca atgtgtttcc
     1861 atttgtttgt cggtcgacgg cattataacg ccgtatcgga aatggcggaa tatgtaaacg
     1921 gattgaaatt ttcgggaaag cagattgtat aagccattta aaacaaatgg ttatttttat
     1981 tgtcggcagt ttgccgcctt ggatggggca gggacttgcg gtagaatccg cttccgattt
     2041 atgggattga cgcatacaga gaattgaaaa catggcaaaa atgatgaaat gggcggctgt
     2101 tgcggcggtc gcggcggcag cggtttgggg cggatggtct tatctgaagc ccgagccgca
     2161 ggctgcttat attacggaaa cggtcaggcg cggcgacatc agccggacgg tttctgcaac
     2221 aggggagatt tcgccgtcca acctggtatc ggtcggcgcg caggcatcgg ggcagattaa
     2281 gaaactttac gtcaagctcg ggcaacaggt taaaaagggc gatttgattg cggaaatcaa
     2341 ttcgacctcg cagaccaata cgctcaatac ggaaaaatcc aagttggaaa cgtatcaggc
     2401 gaagctggtg tcggcacaga ttgcattggg cagcgcggag aagaaatata agcgtcaggc
     2461 ggcgttgtgg aaggatgatg cgacttccaa agaggatttg gaaagcgcgc aggatgcgtt
     2521 tgccgccgcc aaagccaatg ttgccgagct gaaggcttta atcagacaga gcaaaatttc
     2581 catcaatacc gccgagtcgg aattgggcta cacgcgcatt accgcaacga tggacggcac
     2641 ggtggtggcg attctcgtgg aagaggggca gactgtgaac gcggcgcagt ctacgccgac
     2701 gattgtccaa ttggcgaatc tggatatgat gttgaacaaa atgcagattg ccgagggcga
     2761 tattaccaag gtgaaggcgg ggcaggatat ttcgtttacg attttgtccg aaccggatac
     2821 gccgattaag gcgaagctcg acagcgtcga ccccgggctg accacgatgt cgtcgggcgg
     2881 ttacaacagc agtacggata cggcttccaa tgcggtctac tattatgccc gttcgtttgt
     2941 gccgaatccg gacggcaaac tcgccacggg gatgacgacg cagaatacgg ttgaaatcga
     3001 cggcgtgaaa aatgtgctga ttattccgtc gctgaccgtg aaaaatcgcg gcggcaaggc
     3061 gtttgtgcgc gtgttgggtg cggacggcaa ggcggcggaa cgcgaaatcc ggaccggtat
     3121 gagagacagt atgaataccg aagtaaaaag cgggttgaaa gagggggaca aagtggtcat
     3181 ctccgaaata accgccgccg agcaacagga aagcggcgaa cgcgccctag gcggcccgcc
     3241 gcgccgataa acgaatatgc cgtctgaaca cggaaacggt ttcagacggc atttgttatt
     3301 gatttacgga atattatgag cttgatcgaa tgtaaaaaca tcaaccgcta tttcggcagc
     3361 ggcgagaacc gcgtccatat tttgaaagac atcagcctgt cgatagagaa gggcgatttt
     3421 gtcgccatca tcgggcagtc cggttcgggc aagtccacgc tgatgaacat actcggttgt
     3481 ttggataccg ccggttccgg ttcgtaccga atcgacggca tcgaaactgc caaaatgcag
     3541 cctgacgaac tggcggcatt gcgccgcgaa cgcttcggct ttatcttcca acgctacaac
     3601 ctcttaagct cgctgaccgc aagggataac gtcgcgctgc cagccgtcta tatgggcgcg
     3661 ggcggcaaag agcgttccgc gcgggcggac aaactcttgc aggatttggg tttggcaagc
     3721 aaagagggca acaagcccgg cgaactctcg ggcggacagc agcagcgcgt ctccatcgcc
     3781 cgcgccctga tgaacggcgg agaaatcatc ttcgccgacg agccgaccgg cgcgctcgat
     3841 accgccagcg gcaaaaacgt gatggaaatc atccataagc tgcacgaagc cgggcatacc
     3901 gtcattatgg tcacgcacga ccccggcatc gccgccaatg ccaaccgcgt catcgaaatc
     3961 cgggacggcg aaatcatttc cgacacctcg aaaaatccag aaatccccgc aagcaatgtc
     4021 gggaggattc aggaaaaagc ctcgtggtcg ttttattacg accagtttgt cgaagccttc
     4081 agaatgtcgg tgcaagcagt attggcgcac aaaatgcgtt cgcttctgac gatgctcggc
     4141 atcatcatcg gtatcgcttc ggttgtctcc gtcgtcgcat tgggcaatgg ttcgcagaaa
     4201 aaaatccttg aagacatcag ttcgataggg acgaacacca tcagcatctt cccggggcgc
     4261 ggcttcggcg acaggcgcag cggcaggatt aaaaccctga ccatagacga cgcaaaaatc
     4321 atcgccaaac aaagctacgt tgcttccgcc acgcccatga cttcgagcgg cggcacgctg
     4381 acttaccgca ataccgacct gaccgcttct ttgtacggcg tgggcgaaca atatttcgac
     4441 gtgcgcggac tgaagctgga aacggggcgg ctgtttgacg aaaacgatgt gaaagaagac
     4501 gcgcaggtcg tcgtcatcga ccaaaatgtc aaagacaaac tctttgcgga ctcggatccg
     4561 ttgggtaaaa ccattttgtt caggaaacgc cccttgaccg tcatcggcgt gatgaaaaaa
     4621 gacgaaaacg ctttcggcaa ttccgacgtg ctgatgcttt ggtcgcccta tacgacggtg
     4681 atgcaccaaa tcacaggcga gagccacacc aactccatca ccgtcaaaat caaagacaat
     4741 gccaataccc aggttgccga aaaagggctg accgatctgc tcaaagcgcg gcacggcacg
     4801 gaagatttct tcatgaacaa cagcgacagc atcaggcaga tagtcgaaag caccaccggt
     4861 acgatgaagc tgctgatttc ctccatcgcc ctgatttcat tggtagtcgg cggcatcggc
     4921 gtgatgaaca tcatgctggt gtccgttacc gagcgcacca aagaaatcgg catacggatg
     4981 gcaatcggcg cgcggcgcgg caatatcttg cagcagtttt tgattgaggc ggtgttaatc
     5041 tgcgtcatcg gcggtttggt cggcgtgggt ttgtccgccg ccgtcagcct cgtgttcaat
     5101 cattttgtaa ccgacttccc gatggacatt tcggcggcat ccgttatcgg ggcggtcgcc
     5161 tgttcgaccg gaatcggcat cgcgttcggc tttatgcctg ccaataaagc agccaaactc
     5221 aatccgatag acgcattggc gcaggattga ggttggcaca aagatgccgt ctgaagctgc
     5281 aggaccggtc attttggagc agaaacttat tggacaaaaa cggtttctta gattctacgt
     5341 tctagattcc cacttgcgtg ggaatgacgg cggcgggggt tcgatgattg cacacaacgc
     5401 tcgagtcccg tcattcccgt aaagacggga attcggttcg ttcggctttg cttgtttcgg
     5461 ataaatcacg gtaactcaat attccagatt cccgcccgcg tgggaacggc ggcggggctt
     5521 cgtattgttc aatttattat tttcaatcat tcaatgggtt aggatgtgtt tgttggcttg
     5581 ctaactttca gtgcggattg gttttcagac ggcatttccc gcaaaaaagg cttggaattt
     5641 tccaagcctt ttgttttgcg gatggattat tgatttttgc ggatgatttc ctccagttgg
     5701 ggcatcgggc tgtagccgct ttggctgcgc ccgttgggga agacgagggt cggcgtgccg
     5761 ttgaagccga attgttcgcc caaggaagtg gtttccgcga cgggattgtc gcagatgctg
     5821 ccgccgaccg ggaatttgcc tttacgcatc caatccgtcc acgctttggc gcggtcgggc
     5881 tgacaccata agatttgcgc cttgcgcgcg gcatcggggt gcaggccggc aatgggcatc
     5941 ataaagctgt aaaccgtcac gtcggtcatt ttttcaaact cgtgttccaa gcgtttgcag
     6001 aacggacaat cggggtcgga gaagacggcg actttcagct tgccgttgcc gcgcacttct
     6061 ttgatggctt tgtccaaagg cagggaggcg aagtcgattt tgttcaaatc ggcggcgcgt
     6121 tcttcggtca ggtttttgcg cgtgtcgatg ttgatgagtt cgccgacgaa catatagccg
     6181 ccttcggcat cggtgtagat aatctgcctg ccgctgacga cgacttcgta aatgcctttg
     6241 accggtgttt cgctgacgct caacactttc aaatcttggg cggaataggt tttttccaaa
     6301 cgcgctttca aagaggcggc aacggatttg ccggcggact cggctttgac ggcgggttcg
     6361 gcgttggcat tggaaacggg cgtttgcccg caagccagca gtgggaggac ggtaaagggg
     6421 gtcaagattt tgattaactt ggttttcata taaagatgat tgcgcgtgtt ggaaaagcgg
     6481 aattgtatca aatctctgtt gcgcctgcat tgcgcctagg ctcaatttat cgtctgaaaa
     6541 tagcttccgc ctgttaaaat acgcaaaaaa atgatttgcc tgcctgtatg atctaccacc
     6601 gcatcgccgt aaacgtgccg ctttcagacg gccttttgac ttattcccat tccgatccgc
     6661 ttcctccggg aacgcgggtg cttgtgcctt tccgtaataa aaccgttgtc gggattgtgt
     6721 gggaaacgga tattgcgccc gatatggata cggcgcggat tttgagtgtt cagacggcct
     6781 ttatggaaga aaagccgttg cctcaaagct ggcgtgattt gttggcgttt acgtcgcgtt
     6841 attaccacta tccgactggg caggcggtgt ttgccgcgct gccgcagggt ttgaaggaaa
     6901 cgcgcgcggt ggaaatgccg cagccgccgt tgttttatgc tttgaacgaa gcgggcaggg
     6961 cgcaaacgcc gccgccggcg cggtttaaca aaaaagcggc tttgtgggac gcgctgcttt
     7021 cgggcggaat gacgatggcg gcgttgaagc aggtaaacgc gcaggcggcg aaattgattg
     7081 aagattgggc ggaacagggt tggattgaaa cgacggaggc ggcgaaaccg gtattgaggt
     7141 cgtaccacgg gcaggcttcg cactctgaat ttgtattaaa cgccgaccag cagaaggctt
     7201 ccgatgaaat tcagacggca ttgggacgct tccggccgtt tctgctgtac ggcatcaccg
     7261 gcagcggcaa gaccgaagtg tatttcgatg cgatggcgaa agtgttggcg caggggcggc
     7321 aggtgttgtt tctgttgccc gaaatcaacc tcacgccgca gcttttaaag cgggtggaaa
     7381 accgttttgc tgacgtgccg accgccgtgt tgcacagcca gatggcggca ggcaggcgca
     7441 cgcaggatta tttgcgcgcg atgttggggc aggcgaaatt ggtcatcggc acgcggctgg
     7501 cggtgttcac gccgatggat gatgtcgggc tgattgtggt cgatgaggaa cacgacggct
     7561 cgttcaaaca ggacaacgaa ttacgctacc acgcccgcga tttggcggtg tggcgggcga
     7621 agcagagcgg ctgccccgtt gtgttgggca gtgctacgcc cagtttggag agttggcaca
     7681 aggcgcaaag cggcgcgtac cgcctgctgc aactgaccga acgcgcccat accgccgcgc
     7741 aactgccgca agtggacatc ctcaacgtag gtcgtctgaa actcgacaac ggcttctcgc
     7801 cgcaagcctt gcagcttttg aaacagaact ttgaagcagg cggtatgtcg ctggtgtacc
     7861 tcaaccggcg cggtttcgcg cccgcgctgt tttgcggcga ctgcggccat accttcggct
     7921 gcccgaactg ctccgccaaa atggtgttgc accaacgcgc ccgccaattg cgctgccacc
     7981 actgcgacca ccgcgaaccc attccgttca aatgccccga ctgcggcaac caagacctga
     8041 ccgccgtcgg acacggcacg cagcgcgtcg aagaaaccct gcgcgccttc ctgcctaaag
     8101 ccgccgtcgt ccgcgtcgat agggacagca cggcgcacaa aaatgactgg gcggatttgt
     8161 accgccgtat cgccgacaac gaaatcgaca ttctggtcgg cacgcagatg ctcgccaaag
     8221 gtcatgattt cgcgcggctc aacctcgtta tcgtattgaa cgccgatggc agcctgtata
     8281 gcgcggactt tcgtgcgccg gaaaggctgt ttgccgagct gatgcaagtg tccggcaggg
     8341 cggggcgtgc cgacaaaccc ggcaaggtgt tgatacagac ccaactgccc gaacatcccg
     8401 tcttcgccgc cgtcaaggcg caggattatg ccgtgtttgc cgaaaatgaa ttgaacgagc
     8461 ggcaaatgtt cgcgatgccg cccttcggtt tccagaccgc cgtccgcgcc gatgcgccgc
     8521 gtgttgccga tgcgatggaa tttctcaatg ccgccaaaga aaccctcgcc ccgctgttgc
     8581 ccgaaagcgt ttcccagttt ggcgccgccc ctatgctgat ggtgcgcctt gctgaacgcg
     8641 aacgcgcgca aatcttcctc gaatcggtat cccgacagga tttgcaccgc gccgtgagtt
     8701 tgtgggtgca ggtgttgcag caaaaccgcg acagcaaaat cagatggtcg gtggatgtcg
     8761 atccgcagga ggcttgatta tcggcaatac gatgccgtct gaaaccgttt cagacggcat
     8821 ttttattccg ggtcgcccgt aaacgcattc gcccgaaaaa tcggaacaaa cgtgaaaaga
     8881 taaagcgtga acacgacggc ggtcagaatc gcggggacgg tgatgaagaa tatcgggttc
     8941 acgttcatta aaacagcgcg cgaaacggcg gcggcgaaaa gaatggggat ggcgatgcgg
     9001 cagagtttgg ggtagtcaag tttggtaaag ccgctgtgcc acagtccggc agtcagccac
     9061 accatcatca cgccgcccat catgccgccg agagtaatca ggtgcagggg cgcggaggag
     9121 ggcagatttt gcagtttcgc cgcgcctgtc cacagatagc ctgcggcggc aaagagttgg
     9181 agtaggtaat aggtgcggac gtagtgtttg cgcaggagtt cgtgatggtg cagttcgcgc
     9241 agcttggcga gcaggatgaa accgacggcg aatgcggtaa aaccggcggt ttgcgcgggc
     9301 agccaaagtt cggcggcggc gtgcagcagc aggaaggtga tggcgatgtt tttatagacg
     9361 acgttgggga tgaatacggg gtctttcaga cggcattctt tcaacgcttc cgcacccagc
     9421 aaaacactca cgcgtatgga aacaaacatc accgccgcca tattcaaatg cacttgcgcg
     9481 cgcagtaagt tcaaatcgcc gctgacggca taggccgtct gaaaaacggt aaatgcggca
     9541 agcaacgtca aaagagcgaa gttgtcggtg ttgcggtcga gccaaatcag ccgggcgcag
     9601 aacagcagca ataccagcca ataggcggca acgaaaaacg aggcagtttg cggcgaaaag
     9661 ggcagtatag cggatgcggc gagcaataat gccgccatca aagtcgcgac aggtttcagg
     9721 ttacccgaaa aaaaccgtcc agtccaacaa agccgcagtc aggaaaccac catatgccgc
     9781 aggtagcatg agttctaaga agatttggcg gtgcaggacg acggcaccgg ggctgatgaa
     9841 aaacaccaac gcgccgagta tggcaagcac tgtcgcgccg acgaaaaacg ggcgcatagc
     9901 aactgtattt ttcaccccgt cgggcaaaaa taccaaaact caaatcaagc cgtccggata
     9961 ccgttttcgg cggtatcgtt ttcggcggta tcgttttcgg caaaataatc acgcatccgg
    10021 gcattcgata tcgtcagcag tttacgcata cataccgtaa cggcaacctt atacggctta
    10081 ccctcggaca gcaggcgttg gtagaaatcc cgaataagcg gttcaaaacg tgtcgctgcc
    10141 acggtagcca tatacagtgc cttaagcacc gcagaccttc cgccaaagca gcggcttttg
    10201 aatttggttt ccccgctctc cctcgggtgc ggggcaatgc cgaccaaact cgctatccgt
    10261 ttgtgcgaca gccgccccaa ctcaggcagc atcgccatca gcgtagccgt cgttatcgaa
    10321 ccgatgcctt tgatttgttc tgccacttgg gctttgccgt caaaatgcgt g
//
LOCUS       DAWHRS010000066        10328 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWHRS010000066 DAWHRS010000000
VERSION     DAWHRS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.066"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            90..299
                     /locus_tag="WIB82_001174"
     CDS             90..299
                     /locus_tag="WIB82_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ3842475.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            388..1602
                     /locus_tag="WIB82_001175"
     CDS             388..1602
                     /locus_tag="WIB82_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ3842476.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVYQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1689..4268)
                     /gene="clpB"
                     /locus_tag="WIB82_001176"
     CDS             complement(1689..4268)
                     /gene="clpB"
                     /locus_tag="WIB82_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ3842477.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LEKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4529..5539
                     /gene="trpS"
                     /locus_tag="WIB82_001177"
     CDS             4529..5539
                     /gene="trpS"
                     /locus_tag="WIB82_001177"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ3842478.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5545..5886
                     /locus_tag="WIB82_001178"
     CDS             5545..5886
                     /locus_tag="WIB82_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842479.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEEGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6206..6571
                     /locus_tag="WIB82_001179"
     CDS             6206..6571
                     /locus_tag="WIB82_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842480.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAAKDTL
                     NKAADATQEAADKMKDAAK"
     gene            complement(6780..8288)
                     /gene="ppx"
                     /locus_tag="WIB82_001180"
     CDS             complement(6780..8288)
                     /gene="ppx"
                     /locus_tag="WIB82_001180"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ3842481.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(8404..9136)
                     /locus_tag="WIB82_001181"
                     /pseudo
     CDS             complement(8404..9136)
                     /locus_tag="WIB82_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
     gene            9581..10255
                     /locus_tag="WIB82_001182"
     CDS             9581..10255
                     /locus_tag="WIB82_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ3842482.1"
                     /translation="MQADLAYAAERITHDYPQATGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFSGWRIAADYASYRKWKESNFSTKKVTKEINDNYQETKTEHQGNGSFHASSSL
                     GLSAIYDFKLNDKFKPYIGARVAYGHVKHQVHSVETKTTTVTSKPKNGSPQGGAVPSR
                     ESSKPPYHESNSISSLGFGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSL
                     GMRYHF"
BASE COUNT         2439 a         2712 c         2661 g         2516 t
ORIGIN      
        1 cgatttgaat ttttcagaaa atgaagcgga cgtttgggcg gaggcaactt gtttgataag
       61 atagcaatat ttcaaaacgg agaaagatca tgccttatgt caatattaaa gtaaccggcg
      121 gcaaggaagc accgactgcc gctcaaaaag cggaactgat cggcggcgta accgaattgc
      181 tggcacgcgt gctgggcaaa aatcccgaaa caacggttgt cgtgattgat gaagtggata
      241 ccgataactg gggaataggc ggcaaaagcg tcagcgaacg gcgcaaagag ggcaggtaaa
      301 aagcctgaaa atctcggttt gatgctttaa attccgcgtg aaaaagagta cattcccacc
      361 cattgcccaa aatttacgga acacatcatg gataaatttc ccaagtctgc aaagctcgat
      421 cacgtctgtt acgacatacg cgggccggtt cacaaaaaag cacttcagtt ggaagaggag
      481 ggcaataaaa tccttaaact caatatcggc aaccctgcgc cgttcggctt tgaagcccct
      541 gatgaaatct tggtcgatgt catccgcaac ctgccgactt cgcaaggcta ttgcgattct
      601 aaagggctgt attccgcccg caaagccatt gttcactact atcagaccaa aggtttgcgc
      661 gatattacgg ttgatgatgt ctatatcggc aacggcgtgt ccgagctgat tacgatgtct
      721 atgcaggcgt tgctcaacga cggcgacgaa atcctgattc ccgcgcccga ctaccctttg
      781 tggacggcgg cggcaacgct tgcgggcggt acggtacgcc attatctgtg cgacgaagaa
      841 aacggctggt tccccaacct tgccgatatg gaagccaaaa tcacgcccaa aaccaaagcc
      901 atcgtcgtca tcaatcccaa taatccgaca ggcgcggtgt acagcaggga aatcctgttg
      961 gaaatcgccg aactggcgcg caagcacggt ttgattattt tcgccgacga gatttacgac
     1021 aaaatccttt atgacggcgc ggttcaccac catattgccg cgcttgcccc cgaccttttg
     1081 acggtaacgt tcaacggttt gtccaaatcc taccgcgtcg ccggcttccg ccaaggctgg
     1141 atggtgctca acgggccgaa acatcacgca aaaggttaca tcgagggttt ggatatgctc
     1201 tcgtccatgc gcctgtgtgc caatacgccg atgcagcacg cgattcagac ggcattgggc
     1261 ggttatcaga gcattaacga attcattttg cccggcgggc ggcttttgga acagcgcaac
     1321 agggcgtggg aactggtcaa ccagattcct ggcgtgtcct gcgtcaaacc gatgggtgcg
     1381 atgtatatgt tcccgaaaat cgataccgaa atgtaccgca tccgcgatga catgaaattc
     1441 gtttacgatt tgctggtgcg cgaaaaagtc ttgctggtgc agggaacggg gtttaattgg
     1501 atcaagcccg accatttccg cattgttacg ctgccttacg tctatcagat tgaagaggcg
     1561 atgggcaggc tggcaagatt cctgcaaacc taccgccaat aaggggacgg tttgtctgcc
     1621 gaggataaaa aatgccgtct gaaacggaga ttcccgtttc agacggcatt ttcaacagca
     1681 ggaacgaatc aggcaaattt cagtctgtcg ccgtcggctt ccaccctgat ttcgctttcg
     1741 ggcgcatagt ttccggcaag cagggctttt gccagcgggt tttcgatttc cgactggatg
     1801 gcgcgtttga gcggacgtgc gccgtaaatc gggtcgaaac cggctttggc gatgatgtcc
     1861 agagcggcat cggaaacagt caggcggagg ttttgttttt ccaaacgttt ttccaagcct
     1921 ttgagctgga ttttcgcaat gttgcggata ttatcctgat ccagtccgtg gaacacgacc
     1981 acttcgtcga tgcggttgat catttcagga cggaaatgtt ctttcacatc ctccatcaca
     2041 acttctttta ccgcttcgta atcctgaatg cccatttgtt ggatgtgctg gctgccgatg
     2101 ttggaagtca tcacaatcac ggtattttta aaatctaccg tcctgccttg cccgtcggtc
     2161 aaacgaccgt catccaatac ttgcagcagg atgttgaaca cgtcgggatg ggctttttcc
     2221 acttcgtcca aaagaatcac gctgtacggt ttgcggcgga cttgctcagt caggtagccg
     2281 ccttcttcgt agccgacata gcccggaggc gcgccgatta agcgggcaac ggcgtgtttt
     2341 tccatatatt cggacatatc gatacgaatc agatgatctt cgctgtcgaa cagaaagcct
     2401 gccagggctt tacacaactc ggttttaccc acgccggtcg ggcccaagaa caggaagctg
     2461 ccgtaaggct tgttcggatc ggcaagaccg gagcggctgc ggcggatagc gtcggacacg
     2521 gcacgcacgg cttcgtcctg tccgaccacg cggcggtgca atacttcttc cattttcagc
     2581 agtttgtcgc gttcgccttc catcattttg gatacgggaa tgccggtcat acgggaaacc
     2641 acctctgcga tttcctccgc gccgacatta ttacgcaaga gtttgtttgc cggttttgtg
     2701 ctgtccgtat ctgcccgttc ggcggctgca cgctgttttt ccaaatgctc caaatcttca
     2761 tacatcaatt ttgaagccag tgccaaatcg ccttgccgtt ttgcctgttc gattttaatt
     2821 ttgacttcgt caatttgttt cttaatatta gcagcaccgt ctgaaattgc tttttcggct
     2881 ttccagattt cgtccaaatc ggcatattct ttttccaaac cgtcgatttc ctcatctatc
     2941 agttccaaac gttttttgct ggcatcgtct ttttcttttt caacgtgcgc cttttccatc
     3001 cgaagctgaa ttagacggcg gtcgattttg tccattgctt ccggcttggt ttctttttcc
     3061 atcttgacac ggctggcggc ttcgtcaatc aaatcaatcg ctttatcagg caggaagcgg
     3121 tcggtaatgt agcggtcgct caactccgct gcggcaacga tagcagggtc ggtaatatcg
     3181 ataccgtggt ggatttcata acgctcctgt aaaccgcgca agatagcgat ggtgtcttct
     3241 acacttggct cgccaaccaa tactttttgg aagcggcgtt cgagtgccgc gtctttttcg
     3301 atgtattggc ggtattcgtc caaggtggtc gcgccgatac agtgcaattc gccacgtgcc
     3361 aaagccggtt tcagcatatt gcccgcatcc atcgcaccgt cggttttgcc tgcgccgacc
     3421 aaagtatgga tttcgtcgat gaaaatcaga gtgttgccgt cgtctttcgc caagtcgttc
     3481 aacacgcctt tcaagcgttc ttcaaattcg ccgcggtatt tcgcgccggc aatcaatgcc
     3541 gccaagtcca aaaccagtag gcgtttgttg cgcagggatt caggtacttc gccattgacg
     3601 atgcgttgtg ccaaaccttc gacgatggcg gttttaccca cgcccggttc gccaatcagc
     3661 acagggttgt ttttggtacg gcgttgcaat acctgaatcg cacggcggat ttcgtcgtca
     3721 cggccgataa cggggtcgag cttaccgtct cgggcgcgtt gggtcagatc gagcgtatat
     3781 tttttcaaag catcgcgttg gtcttcggca ttggcatcgt tcacgttttg tcctcctcgt
     3841 accgcgtcaa tcgcggcatt gatgttttgt tcggtcgcgc cggcttcttt caaaattttg
     3901 ccggccgcat cgttctgctg caccaaggca agcaggaaaa gttcactggc gatataggca
     3961 tcgccgcgtt tggttgcggc tttgtccatc aggttcaaca ccgcctgcaa ttcgcggctg
     4021 ggcagaatgt cgccgccctg gccggacact ttcggcaggc tgttcaaatg ctgctgcaaa
     4081 cgctgtttca cctgcggcac gttcacgccc gcatgagcca agagcgcggc agcaccgctg
     4141 ttttggtcgt caagcagggc tttcagcaca aagcccgctt ccagatagct gccgtccgca
     4201 gccaacgcca aactctgagc ttctgcaagg gcctgttgga atttggcggt taatttgtcg
     4261 tatcgcattt ttgtttcctt ttcaaaatgt ccgctgtcga agcctatatg tgcataattg
     4321 tggataactc aagttctgtt ttctgttttt ctatatttaa ttcgatatat cattgaattt
     4381 aaagtatata aaaatgtata ataatgtgta taactatatc ttcttaatat ggaaaagtct
     4441 gttgtcggct ggatgtaggt ggcaaatcgg gtataatcgg cgcatctttt tccctttcag
     4501 acggcattga tgccgcaagg acatttttat gagcaaaaaa cgagttctga ccggcgtaac
     4561 caccaccggc atcccgcatc tgggcaacta cgtcggtgct atccgccccg ccgtccgcgc
     4621 ggcgcaaaac cctgataccg aatccttcct cttcctcgcc gattaccacg gtatcatcaa
     4681 atgccacgag ccggagatga ttcaccaatc cacccaagcc gttgccgcca cttggcttgc
     4741 ctgcggactc gaccccgagc gcaccacctt ctaccgccaa agcgacattc ccgaagtgat
     4801 ggaattgaac tggattctga cctgcatcac cgccaagggt ttgatgaacc gcgcccacgc
     4861 ctacaaagcc gccgtgcagg caaatgcgga aaacgggcag gaagaccccg atttcggcgt
     4921 ggaaatgggt ttgttcagtt atccgattct gatgactgcc gatattctga tgttcaacgc
     4981 caacgaagtc cccgtcgggc gcgaccaaat ccaacacgtc gaaatggcgc gcgacatcgc
     5041 cggccgcttc aatcaccgct tccgagaact cttcaccctg cccgaagtga aaatcgacga
     5101 aaacgtcgaa ctcttggtcg gtttggacgg acgcaaaatg tccaaatcct acggcaacac
     5161 cattccgctt tgggaaaacg acaaaaaaac ccaaaaatcg gtcaacaaaa tcatcaccaa
     5221 tatgaaagag ccgggcgagc cgaaacagcc cgacgaaagc ccattgtttg aaatctacaa
     5281 agccttctcc acgccgtctg aaacggtgga atttacgaaa atgcttgccg acggcctggc
     5341 ttggggcgag gcaaaaaaac ttttggcggc gaaaatcaac gccgaactcg ccgaaccgcg
     5401 cgaacgctac aacgagctga ccgccgaccc ttcgcaaatc gaagagattt tgcaggcagg
     5461 cgcgcaaaaa gcgcgtaaag aagcacgcga attgttggac aaagtacgcg atgcggtcgg
     5521 catccgcccg ttgaaataaa cccgatgccg tccgaacctc ttaaaggctt gccggaacaa
     5581 aacgtcgtcc ggctgaccgg caagcatccc aacgacttgg aagccgtcag cggcaaatgc
     5641 atggaaaccg acgataaggg cggtccggta ggctgggcgg aaaacggcgt gtgccatacc
     5701 ttgtttgcca aactggtggg caatatcgcc gaagaaggcg gcaaactgac ggattacctg
     5761 atttcgcatt ccgccctgca accctatcag gcaggcaaaa gcggctatgc cgccgtgcag
     5821 aacggacgct atgtgctgga aatcgacagc gagggggcgt tttatttccg ccgccgccat
     5881 tattgaggta ttcggacatc ccggaatata tttgggtttt tcaaaccttg cgaaaaaaag
     5941 tccgcaccgc agggaaactc aaaaggaggg ggatatgtgt tacaattttc cgaactgttt
     6001 tcataaaata gttttcggac gtgtttcaat atggcattga tgccgcctta ttgttcgaga
     6061 aaaaaacctt tatatttaaa tataatgggt tttaactaaa cgggaaaccg ttttctctcc
     6121 ggccgatggg caaaatcagc cgattgatgg aacacggttc gatttttaag caaaacctta
     6181 atcaacaact caagaaggat aagacatgaa aaaattattg attgccgcaa tgatggcggc
     6241 tgccttggca gcttgttcgc aagaagccaa acaggaggtt aaggaagcgg ttcaagccgt
     6301 tgagtccgat gttaaagaca ctgcggcttc tgccgccgag tctgccgctt ctgccgtcga
     6361 agaagcgaaa gaccaagtca aagatgctgc ggctgatgca aaggcaagtg ccgaggaagc
     6421 tgtaactgaa gccaaagaag ctgtaactga agccaaagaa gctgtaactg aagccaaaga
     6481 agctgtaact gaagcagcta aagatacttt gaacaaagct gccgacgcga ctcaggaagc
     6541 ggcagacaaa atgaaagatg ccgccaaata atttgttgcc ttggcaaaaa tgatgggatg
     6601 ccgcctgccg gcaacccaaa cgaaaccgcc tgaagatttt caggcggttt ttgagttgta
     6661 tgccccttgt tttttacgcc tgcatgaccg ttccgagcag atgtgccgcg catatcggga
     6721 tgttgcggac aggcaaatgc cgtctggaca gggtttggac ggcatttgtt ccgtaaggtt
     6781 cagacgacct cgactttaaa cggcatattg attttttgcc attggacgct ttcatattcc
     6841 aatgcgtcgg caatcagggg atggcgttcc agccattccc tgtcaatacg caggatgaag
     6901 ccgcagcttt ccgtatccgt gcgcaactgc atatttttcg ggaaagacag gtcttggcgc
     6961 gaacggcaga acagggcagc aaggcgcagg gacaaaacgg cataccacaa catttcgttg
     7021 gtgccgatga tgccgctcat ttttttcata tcgccgcgat gaccgatgac cagttgggca
     7081 agtatggtct gttctttgcg tgagaaaccc ggcatatcgg cgttttcgag gatgtaggcg
     7141 gaatgcttgt gatagccggt gtgggcgatg tccaaaccga tttcgtgcag cgcggcggcg
     7201 cgtccgagat actgttgcca caaggcaagc tcttgaactg taacgttttt agcgtggcag
     7261 aggctgtcca taaaggtttg cgcggtctcg gcggtgcgtt tcgcctgatt gaggctgacg
     7321 tggtagcggt gttggaactc ggcaaccgtt tgtccgcgca tatcttcgtt taaaccgcgc
     7381 ccgatcaaat cgtaaaacac gccgtcgcgc agggcggctt cggttacggt catcctgtcg
     7441 agtttcattt cctcaaacgc cgccatcatc acggcaagtc cgccggcaaa aacttcgatg
     7501 cgttccggtt tcaggttttc aaatttggct tttttgaccg aaccggcttc gatgatgcgt
     7561 tcggcgaggg cgcgcatgcc tttgtaggta atgtccgcct cttggggcat ttcggcggca
     7621 agcacgtcgc ggatggattt tgccgaaccc gatgtgccga cggcgaaatc ccaaccttcg
     7681 cgcctcatat ttttgctgat gcgctggatt tcgttgcggg cggcggaaat ggcagattgg
     7741 aagtctttgg cggtgatttt gttttggaag aagcgcaggc tgtaggttac gcagcccaag
     7801 ggcaggcttt cggtaatgtc gggattcagc gtcgagccga tgacaaattc tgtcgaaccg
     7861 cccccgatgt cgataaccag cattttgccg ccgcccgggg ggagggtgtg gatcacgccg
     7921 gtataaatca gccgcgcctc ttcgcgccct gcgatgattt cgatggggaa acccagtgcc
     7981 gcttcggcct tgggaaggaa atctgcgatg tttttggcga cgcggaatgt attggttgcc
     8041 acggcgcgca cctgttcagg gcggaagccg cgcaggcgtt cgccgaattt tgccaggcag
     8101 tccaaagcct gttcttggga agcggcactc aggtttttct gttcgtccag accggcggcg
     8161 aagcgcacca tctgtttgaa cgaatcgata acttttaatt gtccgttgtt gttttcgcaa
     8221 atctggaggc ggaaactgtt ggaacccaaa tcgacggagg cgaggacgtt tgcgggatgg
     8281 gtggtcatgg tggataccgg tgggggaaaa acgcaatgtt actctgacgg cgcaggcgtt
     8341 gacaataaat gatgcggcgg tttttgattc tgcccacgga tgttgccgac ggcatttttg
     8401 cgcttatttg aaatcctttt ccacgctcat gaaaatctgc gtgtttttgc gtgtgtaaaa
     8461 acttttcata ttgctgtcga ttttcagata gcggaaattg agttgcggcg taaagccctt
     8521 ccaagagatt ttgtcgtgcc acaacgacag gtttgcctga tattcgtggt ctttgcgcgg
     8581 gaagcggtac acaatggtcc cgggtgcgtc aaacatcctg cgggtatagc gcaggtttgc
     8641 ccgcagaccc aagcccgccg tcgaacgttt tgactgcgcc gacacgcaaa cccttgcgga
     8701 cggaagcctg ttccgcctcc ttcgtcaagt cgtgcgacca gtccgccccg ccgtaaagca
     8761 gccagtcttt cggcgcggaa tacatcagcg tcgcgcccgc cagcggcata tggctgtcgt
     8821 accgggcggc ggtgcggtct tcctgataat gcttccacat attgcccgcg tttagtgtca
     8881 accgccagcg ttcgctcaag tgttgggaga aatcggcatt aaagccgccg acgaaattgt
     8941 atcggctgcc gcctaagagg ttttgcccga caaacggcac gatgccgaac gagcgcgtta
     9001 ccgaacgatt tttatagccg agcgacaggt gcaggcactg ttcgctgaaa tctttgttat
     9061 cccaataatg cacgccgccg ccgctgatgc cgccgtagag gaaatgatgc ccgcccgcat
     9121 tgatttcgcg cgacacgcct aagccctagc gcaaaccgtg tgccttttgc ggcaggctgt
     9181 cggcggtttt cgtccagctt ctgcccgcaa attcaatcgt ttttttggac gaagcgttgt
     9241 ttatagcgga ttaacaaaaa tcaggacaag gcgacgaagc ctcagacagt acagatagta
     9301 cggaaccgat tcactcggtg cttcagcacc ttagagcatc gttctctttt ttgttcatcc
     9361 gctatattgt gttgaaacat cgccccaaac ccgatataat ccgcccttga accatcagtg
     9421 aaaatcgttc tttttaatca gttaaaccga atacggagtc gaaaatgaat ccagccccaa
     9481 aaaaccttct cttctcttct cttctcttct cttctcttct cttctcttct cttccgcagc
     9541 gcaggcggca agtgaagaca gcagccgcag cccgtattat gtgcaggcgg atttagccta
     9601 tgccgccgaa cgcattaccc acgattatcc gcaagcaacc ggtgcaaaca acacaagcac
     9661 agtaagcgac tatttcagaa acatccgtgc gcattccatc cacccccggg tgtcggtcgg
     9721 ctacgatttc agcggctgga ggatagcggc agattatgcc agttacagaa aatggaaaga
     9781 aagtaatttt tctactaaaa aagttactaa agagataaac gacaactacc aagaaaccaa
     9841 aacagaacat caaggaaacg gcagcttcca cgcctcttct tctctcggct tatccgccat
     9901 ttacgatttc aaactcaacg ataaattcaa accctatatc ggtgcgcgcg tcgcctacgg
     9961 acacgttaaa catcaagttc attcggtgga aacaaaaacc acgactgtta cctctaaacc
    10021 aaagaacggc tctccacagg gaggagctgt cccatcaaga gagtccagca aacctcccta
    10081 tcacgaaagc aacagcatca gcagcttggg ttttggtgtc atcgccggtg tcggtttcga
    10141 catcacgccc aagctgacct tggacaccgg ataccgttac cacaactggg gacgcttgga
    10201 aaacacccgc ttcaaaaccc acgaagtctc attgggcatg cgctaccact tctgattccc
    10261 cgacaccgat gccgtctgaa ccttcagacg gcatttttga ttcacctgcc gtttacaggc
    10321 gcggggcg
//
LOCUS       DAWHRS010000067        10298 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWHRS010000067 DAWHRS010000000
VERSION     DAWHRS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.067"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            116..928
                     /locus_tag="WIB82_001183"
     CDS             116..928
                     /locus_tag="WIB82_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ3842483.1"
                     /translation="MNPAPKKLSLLFSSLLFSSLLFSSAAQAASEDSSRSPYYVQADL
                     AYAAERITHDYPKPTGTDKDKISTVSDYFRNIRAHSIHPRVSVGYDFGGWRIAADYAS
                     YRKWNNNKYSVNTKEVLRNNNGNRKELKTENQENGTFHAVSSLGLSAIYDFKLNDKFD
                     KFKPYIGARVAYGHVKHQVHSVKKETTTTFLAPTQGATVPGKIVEGPFSKPAYHESHS
                     ISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
     gene            complement(902..1111)
                     /locus_tag="WIB82_001184"
     CDS             complement(902..1111)
                     /locus_tag="WIB82_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842484.1"
                     /translation="MPSERLSDGIFPCVLNRIHETLDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGIGESEVVAHAQ"
     gene            complement(1129..1686)
                     /locus_tag="WIB82_001185"
     CDS             complement(1129..1686)
                     /locus_tag="WIB82_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ3842485.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1762..3396)
                     /locus_tag="WIB82_001186"
     CDS             complement(1762..3396)
                     /locus_tag="WIB82_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ3842486.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3805..4911)
                     /gene="serC"
                     /locus_tag="WIB82_001187"
     CDS             complement(3805..4911)
                     /gene="serC"
                     /locus_tag="WIB82_001187"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ3842487.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTRDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            5276..5707
                     /gene="rimP"
                     /locus_tag="WIB82_001188"
     CDS             5276..5707
                     /gene="rimP"
                     /locus_tag="WIB82_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ3842488.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            5735..7237
                     /gene="nusA"
                     /locus_tag="WIB82_001189"
     CDS             5735..7237
                     /gene="nusA"
                     /locus_tag="WIB82_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ3842489.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDYLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            7249..10137
                     /gene="infB"
                     /locus_tag="WIB82_001190"
     CDS             7249..10137
                     /gene="infB"
                     /locus_tag="WIB82_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ3842490.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAATRAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAGKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
BASE COUNT         2771 a         2955 c         2568 g         2004 t
ORIGIN      
        1 ttgttcatcc gctatattgt gttgaaacat cgccacaaac ctgatatagt ccgctcctgc
       61 aacatcagtg aaaatcgttc tttttaatca gttaaaaccg aatacggagt cgaaaatgaa
      121 tccagccccc aaaaaacttt ctcttctctt ctcttctctt ctcttctctt ctcttctctt
      181 ctcttccgca gcgcaggcgg caagtgaaga cagcagccgc agcccgtatt atgtacaggc
      241 ggatttagcc tacgccgccg aacgtattac ccacgattat ccgaaaccaa ccggtacaga
      301 caaagacaaa ataagcacag taagcgatta tttcagaaac atccgtgcgc attccatcca
      361 cccccgggtg tcggtcggct acgatttcgg cggctggagg atagcggcag attatgccag
      421 ttacagaaaa tggaacaaca ataaatattc cgtcaacaca aaagaggtgc taagaaacaa
      481 taatggcaac aggaaagaac tgaagacgga aaatcaggaa aacggtacat tccacgcagt
      541 ctcatcgctc ggcttatccg ccatttacga tttcaaactc aacgataaat tcgataaatt
      601 caaaccctat atcggtgcgc gcgtcgccta cggacacgtt aaacatcagg ttcattcagt
      661 gaaaaaagaa accacgacta ctttccttgc accaacgcaa ggcgctacag tgccaggcaa
      721 gatcgtagaa ggtccgttca gcaaacctgc ctatcacgaa agccacagca tcagcagctt
      781 gggtcttggt gtcatcgccg gtgtcggttt cgacatcacg cccaagctga ctttagacac
      841 cggataccgc taccacaact ggggacgctt ggaaaacacc cgcttcaaaa cccacgaagt
      901 ctcattgggc atgcgctacc acttctgatt ccccgatacc gatgccgtct gaaccttcag
      961 acggcatgag acctttgcct gcgtacttgg tgcgctggtc gcctccgaac atggcgcgac
     1021 acccgacatt tcccccgaac gcatcgagcg tttcatgaat ccggtttaaa acgcatggaa
     1081 aaatgccgtc tgaaagcctt tcagacggca tttcgcttga gattccgttt accaatggct
     1141 gacaaacgct tccaaatcgg tattcttggg cttatgcact tcctctgtcg gcgtgccgac
     1201 catcatcagc ccgatgattt tatccttatc cgcacatccg aaagcctcac gcaacagggg
     1261 gctgttgacc cacatccccg taatccagac attgtcgaaa ccttgcgccg ttgccgccag
     1321 ttgcagcgca tacgccgcac aacccgccgt cagcatctgc tcccattccg gtttcggctt
     1381 aggcacatcg cggttcggcg caaacgttac accaatgacc atcggcgcca tattgcccac
     1441 tttttccgcc tttttcatcg catcgtcgcc gaaattcaat tcggcaaccg tttgcttcaa
     1501 cacatcgcga aaacgttgca atcctacctc gccttgaatc acggtaaaac ggaaggggcg
     1561 catattgccg tgatcgggaa cttgggttgc cgcctgaaat atttgttcca actccgccgc
     1621 atcgggggcg gggtgcttca gctttttgga agatcggcgg ttcgtcaata attttaaagc
     1681 atccatatcg ttatactccg gtcatcggtc gggtgttcgg acacatatgc cgtccgaagg
     1741 cttcagacgg catatccggc atcagcgcgg acggcggcag gctgccaata tatccatttc
     1801 cttccgatag gtttggctat tggaaatgtc catcagcccc aataccgtgc tgaaatagtg
     1861 gtcgtgcgaa tattcgtttt ccgccgcttt ttgtttgagg cattggaaat ctatgccccc
     1921 gtgttggcgg aaggctttgg aaaaccacat aaccatcggg atatgcgtct gcccggaagg
     1981 cgcgatggcg taaggcgcgg cgtgcaggta catcccgttt tcgcccaaac tttcgccgtg
     2041 gtcggaaaca taatgcacca cgctttccaa atcgtcgcgg ttttcaagtt tgcggataac
     2101 cttgtcgata aactggtcca catacaaaac cgtattgtcg taagtgttga ccagcgtggc
     2161 gcgggtgcat ttgttgattt cgttggtgtc gcaggtcggc gtgaatttgc gttcggcttc
     2221 ggtatagcgt tcgtaatacg tcggcccgtg gctgccgatg gtatgcagga ttaaaaccgc
     2281 gtctttatcg tttttgttga ggacttcgtc gaacttagtc agcaggatat tgtcgaggca
     2341 ctcgccgttg cggcagtatt cgggcaggtt gagcgaggta acgtcggtat tcggcacttt
     2401 gccgcacacg cccttgcagc cggaatcgtt ttccaaccag gtaacttcca cgccggcgcg
     2461 ctgcacgatg tccagcaggt tgtcttggtg ttcggctttg atttcgtcat aatccgtgcg
     2521 gtcgaaggtt gagaacatac acggcaggga gtgcgcggtc gatgtgccgc agcttctgac
     2581 ctgcgggaaa ttgacaattt catcgccgcg cgcggcaagc agcggcgtag tttggcggct
     2641 gtaaccgttc aaaccccagt tggcggcacg cgtggtctcg cccacgacca gcaccacgaa
     2701 acggcgcagg ctgccggccg gccggttttg cacgaccgcc atatccaatt gcgtataagg
     2761 aatattggaa cgcttccaat ctttgtattt cgacacgccc gcgccgatga aattagacgg
     2821 cacaatcaga tgggttactg atttattgtt gcggaaaaac gaggcgtaat cctgatattg
     2881 caacattgcg atacccaacg cgcacaaaaa ggaaacggcg gcaagcacaa ggcgcgtcaa
     2941 aagctcctta taccaaacgc ggtatttaac cttgacggcg atatacgcca gcgcgggcaa
     3001 tacgcccaaa catacaatcc acagcacata gcccggcgta atcaggcgcg cgctttcggc
     3061 agccgtagtt tgcaagacat tattcaacat cgacttgttg aaatagatat tgaaaaatat
     3121 ttcttggtaa gacaccgccg cactgataac caatatcaac ggaatcaata ccttatgcac
     3181 gaaaggcagg gcaatgacgt gaaaaacgaa attacttaaa aaaaacagca ccaccggcat
     3241 cgtatagagg aagatatccg ccccggtgcc gttaaaagga tgaagctcga caactttggc
     3301 aaaaaaggcg taattcaata ccagcgagga atacagggaa aggaaggcaa tcagcgcgga
     3361 agagccgagc ttcggcctca ggttcggttt tatcatttgg aatgtgtcgg ataagggttg
     3421 gaaaaggcat ccggcatttg gaatccggat tattgaaaaa gattcttaat tataaggcaa
     3481 cggagcaaag cagggcaaga aaacggcggc tgtgcggggg gggggtccgc ccgccattca
     3541 aacgtccggc agacataaaa acatcgtaag caagattcga accggtctgc aaccgcccct
     3601 gccagaaaaa cgggcaaagc atttcatatt ggaaaaaccc ggccgcgccg ccgacgggac
     3661 agtccggcac aaacagcatc acgcccggat tgaaaaggac ggatagccgc cggcagcaca
     3721 gttttacccc gcatcccaaa acgccgccgg agaacgccga aacagccgat gccgtctgaa
     3781 gccgcttcag acaacatcgg gacatcaacc gtaacgccgt tggaaatcgc gcataaaatc
     3841 tgccaaagcc cgcacgcctt cgagcggcat cgcattataa atgctggcac gcatcccgcc
     3901 gacagactta tagcccttca acaggcacaa gccctgcaat tcggcttcca gcacaaaacg
     3961 gcggtcaagc tcctcatccc tcgtttggaa cacgacattc attttagaac gcgcattcgg
     4021 acggatacgg ttgatataga aaccgccgct gccgtctatc gtctcataca aggtttgcgc
     4081 cttcagccta ttgaccgctt caattttttt cacaccgccc tgcgcctgca gccagcggaa
     4141 caccagcccc gacatataaa tcgcgtaagt tgacggcgtg ttgtacatac cgtcgcggtt
     4201 gatgtgcgaa cggtagttga acacatcggg aatatcgttc ggacaacgct cgagcaaatc
     4261 ctcacgcaca atcaccaccg taactcctgc cggcccgatg tttttctgtg cgcctgcgta
     4321 aatcagtccg tagtcggcaa catcaaactc gcgcgacaaa atctcgctgg acatatcgca
     4381 caccagcggc ggcatgcctt ctgaaaggcg cggcacttca cggtattgca gcccgttgac
     4441 cgtttcattg acggcaaaat ggacaaacgc cgaatcgggt gcaacatccc acgtttccac
     4501 aggcggcagg tcgagatagt cgaactgctc gccgccatgc gccgccaaac ggatttccgt
     4561 atcggtcaaa cggctcatct gttcataagc gatacggctc cagttgcccg ttaccaccgc
     4621 gtcggcagtg cggaaaccgt gtgccagatt catggctgcc atattaaatt gggttgttgc
     4681 tccgccctgc agaaacaata tcttatagtt gtcaggcact ttcaaaagct gcctcaaatc
     4741 ctgttccgca tgatgcagga tgctcaaaaa catttccgaa cggtggctca tcgccatcac
     4801 gggaaaaccc gtaccgttgt agtccaacat ttcctgccgc gccgtttcca acacggcttc
     4861 gggcaatacg gcagggccgg cggaaaaatt gtaaatcgga taaagagaca tgatgcagcc
     4921 ttgattctga acaataaccc gcctgatttt aggcttgcag caggctttgt gcaaggatgg
     4981 gaaatacctg tcctccgccc cgcttcatgc cgcccgaaca cggaaaacaa catcaatata
     5041 ttgatttaca aatataaaaa tcatgctagc gacaaataga tacatttgtt ttgtcaacaa
     5101 tattcacgat ttcccattac aaacctccct tacacccgct tttttccgtc ccaaaaacac
     5161 aaaataaatc aacactttca tttctccgca aaagcggtta taatcacgcc gatttttcaa
     5221 ctttgacgaa aaatggccac ggggccattt ttttattatg tacataggga gcagcatgga
     5281 tatccaaacc atcctcgaaa aaaccctgcc cggcctgggc tacgaactgg tcgatttcga
     5341 actgaccgcg caaggaacat tgcgcgtgtt catcgacaaa gaaagcggca ttaccgtcga
     5401 agactgcgca accgtcagca accacttgag ccgcgtcttc atggttgaag acatcgacta
     5461 caaaaacctg gaaatttcca gccccggact cgaccgcccc ttgaaaaaag ccgccgactt
     5521 cgtgcgcttt gccggtcaga atgccaaaat caaaacccgc ctgccgatag acggtcagaa
     5581 aaactttatc ggtaaaatcg aaggctgcga aaatgatacc gttaccatat ccttcgacgg
     5641 caaaaccgta caaatcgaat tgggcaacat cgacaaagcc cgtctgcgcc ccgaattcaa
     5701 attctaaaac acagcaatat tggagatgtt caaaatgagt cgtgaaatgt tacagctggc
     5761 agaagcactg gcaagcgaaa aaaacgttga tgcggaagtc gtcttccaag cactggaatt
     5821 cgccctgtct accgccgcca agaaaaaggc agaccgcgaa cacatggatg tccgcgtaca
     5881 aatcaaccgc gacaccggcg aataccaaac cttccgccgc tggctgattg tcgccgatga
     5941 agactatacc tatcccgatg tcgaaaaaac catcgaggaa atccaagagg aaattcccgg
     6001 cactaccatc caaatcggcg aatactacga agagcagctg cccaacgaag gcttcggccg
     6061 ccaagccgcg caaaccgcca aacaaatcat cctgcaacgc atccgcgatg ccgagcgcga
     6121 gcaaaatctg aacgagtttc tcgccgtcaa agaagacatc gtgtccggca cggtcaaacg
     6181 cgtcgaacgc cacggcatca tcgtcgaagt cgttgccggc aaactggacg cgctgattcc
     6241 gcgcgaccaa atgattccgc gcgaaaactt ccgcagcggc gaccgcatcc gcgccctctt
     6301 cctgcgcgtc gaagaaatcg gcaacaccgg ccgcaaacaa gtcatcctga gccgtacttc
     6361 cggcgatttc ctcgtcaaac tgtacgccaa tgaagtacct gaaattgcag acggcatgct
     6421 tgaaatccgc gctgtcgccc gcgacccggg acaacgtgcc aaagtcgccg tcaaagccaa
     6481 cgaccagcgc atcgatccgc aaggcacctg tatcggcgtt cgcggttcgc gcgtcaacgc
     6541 ggtcagcaac gaattgtccg gcgaacgcat cgacgttgtc ctttggtcgc ccgagcctgc
     6601 ccaattcgtc atgagcgcgc tctcacccgc cgaggtcagc cgcatcgtca tcgacgaaga
     6661 caaacacgcc gtcgatgtca tcgttgccga agaccagctc gcgctcgcca tcggacgcgg
     6721 cggtcaaaac gtgcgccttg cttccgacct gactggctgg cagctcaaca tcatgacctc
     6781 tgcagaagca gacgagcgca atgcggcaga agatgccgcc atccgccgcc tgtttatgga
     6841 ttacttgaac gtggacgaag aaaccgccga cgtactggtt caggaaggtt ttgcaacctt
     6901 ggaagaagtc gcctatgttc ctgccgccga actgcttgcc attgaaggat ttgacgaaga
     6961 aatcgtcgat atgctccgca accgcgcccg cgatgccatc ctgaccatgg cgattgccgc
     7021 cgaagaaaaa ctgggcgaag tatccgacga tatgcgcaac ctcgaaggca tagatgccga
     7081 tatgctccgc agccttgccg aagcaggcat taccacccgc gacgacttgg cagagcttgc
     7141 tgtggacgaa ctgattgaaa tcaccggtgt aaacgaagaa accgcaaaag ccgtcatcct
     7201 gaccgcacgc gaacactggt ttaccgaaga caaataaagg gggtacagat gagtaacaca
     7261 accgtagaac aatttgccgc cgagctgaaa cgccccgtcg aagacctgtt gaaacagttg
     7321 aaagaagccg gcgtcagcaa aaacagcggc agcgattccc tgacgctgga cgacaaacag
     7381 cttctgaacg cctacctgac caagaaaaac ggcagcaacg gcggcaccat cagcatccgc
     7441 cgcaccaaaa ccgaagtcag caccgttgac ggcgtaaaag tcgaaacacg caaacgcgga
     7501 cgcactgtca acattccttc tgccgaagaa ttggcagcac aggtaaaagc cgcccaaacc
     7561 caagccgcac ctgtccggcc ggagcagacg gcagaagacg cggcaaaagc ccgagccgaa
     7621 gctgccacac gtgcagaagc ccgtgccaag gcagaagcgg aagcggcaaa actgaaagcg
     7681 gcaaaagcag gcaacaaagc caaacctgcc gcgcagaaac ccaccgaagc aaaagccgaa
     7741 accgcacccg ttgcggcgga aaccaaaccc gccgaagaaa gcaaagcggg aaaagcccaa
     7801 gccgacaaaa tgccgtctaa aaaacccgcc gagcccaaag aaaaagccgc caagccgaaa
     7861 cacgagcgaa acggcaaagg caaagatgcc aaaaaaccgg cgaaacctgc cgcacctgcc
     7921 gtgccgcaac ccgtggtcag cgcggaagaa caggcgcaac gcgatgaaga agcacgccgt
     7981 gccgccgcac ttcgcgccca ccaggaagcc ctgttgaaag agaaacagga acgccaggca
     8041 cgccgcgaag ccatgaaaca acaggcagaa caacaggcaa aagccgcaca ggaagccaaa
     8101 accggcagac agcgtcccgc caaacctgcc gagaaaccgc aggcagccgc gccggccgtc
     8161 gaaaacaaac ctgtcaatcc ggcaaaagcg aaaaaagaag accgccgcaa ccgcgatgac
     8221 gaaggtcaag gccgcaacgc caaaggcaaa ggcggaaaag gcggacgcga ccgcaacaat
     8281 gcacgcaatg gcgacgacga gcgcgtacgc ggcggcaaaa aaggcaaaaa actcaaactc
     8341 gagccgaacc aacacgcctt ccaagcaccg accgaacctg tcgtacacga agttttggtg
     8401 cctgaaacca ttaccgttgc cgacttggca cacaaaatgg cagtcaaagg cgtggaagtg
     8461 gtcaaagccc tgatgaagat gggcatgatg gttaccatca accaatccat cgaccaagac
     8521 accgccctga ttgtcgtcga agaactcgga catatcggta aacctgccgc agcggacgac
     8581 cccgaagcct tcttggacga gggcgcggaa gcagtggaag ccgaagcatt gccgcgtccg
     8641 cccgtcgtta ccgtgatggg ccacgtcgac cacggcaaaa cctcgctgct ggactacatc
     8701 cgccgtacca aagtggtaca gggcgaagcg ggcggcatta cgcagcacat cggcgcgtac
     8761 cacgttgaaa cccctcgcgg cgtgattacc ttcttggaca ccccgggcca cgaagccttt
     8821 accgctatgc gcgcacgcgg tgcgaaagca accgacatcg tgattcttgt ggtcgccgcc
     8881 gacgacggcg tgatgccgca aaccatcgaa gcgattgccc acgccaaagc ggcaggcgta
     8941 ccgatggtgg ttgccgtcaa caaaatcgat aaagaagccg ccaacccaga gcgtatccgc
     9001 caagagctga ccgcacacga agtcgtgcct gacgaatggg gcggcgatgt acagtttatc
     9061 gacgtttccg ctaaaaaagg cctgaacatc gatgcattgc tcgaagcagt cttgctcgaa
     9121 gccgaagttt tggaactgac tgcccctgtc gatgcacctg ccaaaggtat catcgttgag
     9181 gcacgtttgg acaaaggccg cggcgcggtt gccacattgc tggttcaaag cggtacgctg
     9241 aaaaaaggcg atatgctgct ggccggtacg gcattcggca aaatccgcgc gatggtcgat
     9301 gaaaacggca aatccattac cgaagccggc ccgtccatcc ccgtcgaaat cctcggcttg
     9361 tccgacgtac cgaatgcggg tgaagacgcg atggtattgg cggacgagaa aaaagcgcgc
     9421 gaaatcgccc tcttccgcca aggcaaatac cgcgacgtgc gccttgccaa acagcaggcg
     9481 gcgaagctgg aaaatatgtt caacaatatg ggcgaaaccc aggcccaatc cttgtcggtc
     9541 atcatcaagg cagacgttca gggttcttac gaggctttgg cgggcagcct gaaaaaactg
     9601 tccaccgacg aggtgaaagt gaacgtatta cacagcggcg tgggcggcat taccgaatcg
     9661 gatgtcaacc ttgccatcgc gtccggggcg ttcattatcg gctttaacgt gcgtgcagat
     9721 gcctcttcgc gcaaacttgc cgaaaatgaa aatgtggaaa tccgctacta caacatcatc
     9781 tatgatgcca tcgacgacgt gaaagccgcc atgagcggta tgctttcccc ggaagagaaa
     9841 gaacaggtta ccggtacggt cgaaatccgt caggtcatct ccgtttccaa agtcggcaat
     9901 attgcaggct gtatggttac cgatggtgtg gtcaaacgcg attcccatgt ccgcctcatc
     9961 cgcaacaacg tggttatcca taccggcgag ctggcttcgt tgaaacgcta taaagacgac
    10021 gtaaaagaag tccgcatggg ctttgagtgc ggtctgatgc tcaaaggcta caacgagatt
    10081 atggaaggcg accaactgga atgcttcgac atcgtcgaag ttgcccgcag cctgtaattc
    10141 ctttgcaaat aaaatgccgt ctgaagcctt cagacggcat cggaaaacgg gttctgtatc
    10201 atacagaacc cgttttttgt cgcaaatcgg cttcagcgac cctcctgcct tatcccgatt
    10261 tgaatctgac ttgccataca aacaggcttc agacggca
//
LOCUS       DAWHRS010000068        10037 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWHRS010000068 DAWHRS010000000
VERSION     DAWHRS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.068"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..85)
                     /locus_tag="WIB82_001191"
     CDS             complement(<1..85)
                     /locus_tag="WIB82_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3842491.1"
                     /translation="MLPWEYEDNTLQIKEKSTERTDPFFCTR"
     gene            complement(302..2359)
                     /gene="ppk1"
                     /locus_tag="WIB82_001192"
     CDS             complement(302..2359)
                     /gene="ppk1"
                     /locus_tag="WIB82_001192"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ3842492.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFNPVVEMMREAAADPAVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2528..2971)
                     /locus_tag="WIB82_001193"
     CDS             complement(2528..2971)
                     /locus_tag="WIB82_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ3842493.1"
                     /translation="MPLRLLLGILCDLSMVAPVYAQGQPDAVNAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRRYFVLEVI"
     gene            complement(2978..3493)
                     /locus_tag="WIB82_001194"
     CDS             complement(2978..3493)
                     /locus_tag="WIB82_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ3842494.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACTTKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            3763..6495
                     /locus_tag="WIB82_001195"
     CDS             3763..6495
                     /locus_tag="WIB82_001195"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ3842495.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVLQRVTIGLSPEQEEAMGFGGGSGC
                     GAGYGDGSGSDSRDLPFMPNKWQAWYGVKDDFMVLINSGELDGMNYQTAFDAVAAKLQ
                     AIGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCEQCGDVPVPADQLPVVLPENVVPDG
                     MGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFMESSWYFFRYMSPKFSDGMVDPAAA
                     KYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEGLVNVDEPFERLLTQGMVVCETYYR
                     ENDKGGKDWINPADVELTFDDKGRPVSAVLKVDGLPVVISGTEKMSKSKNNGVDPQEL
                     INAYGADTARLFMMFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGEAVNAFA
                     GSQDGLSKELKDLRHKLHATIAKVSDDYGRRQQFNTAIAAVMELLNQYDKTDTSGEQG
                     RAVAQEVLEAAVRLLWPIVPHICETLWSELNGAKLWEAGWPAVDEAALVKSEIEVMVQ
                     VNGKLRGKITVAADASKADLEAAALATEGAVKFMEGKPAKKIIVVPGRLVNIVV"
     gene            6630..7422
                     /locus_tag="WIB82_001196"
                     /pseudo
     CDS             6630..7422
                     /locus_tag="WIB82_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            7552..7665
                     /locus_tag="WIB82_001197"
     CDS             7552..7665
                     /locus_tag="WIB82_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ3842496.1"
                     /translation="MRDFDKPAASEGAVKFIEGNPAKKIIAVPGRLASIVV"
     gene            7772..8614
                     /locus_tag="WIB82_001198"
     CDS             7772..8614
                     /locus_tag="WIB82_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ3842497.1"
                     /translation="MSFLPSFKRIVCLSAVISILGGCAVVADVYGHDSATMNAVAAEN
                     YMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYADAANNTGHRFDWKMTVFKNDELN
                     AWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTHALHEHGKNKVGQQILTNTAAQI
                     GTQIILDKKPDTNPELVGLGMDILGTYGLTLPYSRSLEEEADEGGMMLMAQAGYHPAA
                     AVRVWEKMNQENDQNGFIYAITSTHPTNNARIENLKRLLPTVMPVYEQSVRNKGRVNK
                     KRRR"
     gene            complement(8951..9262)
                     /locus_tag="WIB82_001199"
     CDS             complement(8951..9262)
                     /locus_tag="WIB82_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ3842498.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(9259..9627)
                     /locus_tag="WIB82_001200"
     CDS             complement(9259..9627)
                     /locus_tag="WIB82_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ3842499.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILLKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
BASE COUNT         2356 a         2576 c         2706 g         2399 t
ORIGIN      
        1 ttcgagtaca aaaaaacgga tcagttcttt ctgtactttt ttctttaatt tgcagtgtgt
       61 tatcttcata ttcccagggt aacatatctg ctaatctagt acagccccta aaatttaatc
      121 cactataatg ccgtctggaa tgcggcggct tggggttggg agcgtatagg ggaagtgctt
      181 gtgcaggaat ggcgaaggtg tgtttttgac tccggtccgc cctgtttaga ttgctgtaaa
      241 accatgccgt ctgaaacgga aaggtttcag acggcatggt ttttgattgg ggcggtgcgg
      301 gtcagcctcc gagcagtgtc cacaaatcgt tttgcaggtc ggcttcggat tcgccctcgg
      361 caggcgcggc gcggatatag ccgccgtcgg gctgcatcag ccacgcgtgc gtattgtctg
      421 ccagtgccat ttccaaacct tcgcggatga cgcgtttttt gagttcgggc gcggtaatcg
      481 gcgcggcggt ttcgatgcgg cggaagaagt tgcgccccat ccaatccgcg ctggagataa
      541 aggtatcgtc cgcgccgttg ttgtggaagc aatacacgcg cgcgtgttcg agctgcctgc
      601 cgatgatgga gcggacgcgg atgttttcgg acaagccttt tacacccggg cgcaaggtgc
      661 acataccgcg cacaatcaaa tcgatttgta cgcctgccgc gcttgcccga tacagggctt
      721 cgatgacggt cggttcgatg agcgaattca tcttggcggt aatccgcgcc ggtttgccgg
      781 cttttgcgtg ttcggtttcg cgtgcgatgc ggtcgataac cattttgtgc agggtaaacg
      841 ggctttggta gagtttgttc agccgcccgg gtttgcccaa gcctgtgatt tccataaaca
      901 atgtgttcac atcggcggtg atttgttcgt cggcggtaat gatgccgaag tcggtgtaga
      961 tgcgcgatgt gccttggtgg tagttacccg tgccgaggtg ggcgtaacgc ttgagtacgc
     1021 cgtcttcgcg gcggatgacc agtgccatct tggcgtggac tttgtagccg aacacgccgt
     1081 acacgacgtg cgcgcccgcc tcttcgagct gcttcgccca gttgacgttg ttggcttcgt
     1141 caaaacgcgc catcagttcg acgacgacgg ttacttgttt gccggcgagt gccgccttca
     1201 tcagggcgcg gacgagttcg gaacgcgtgc cggtgcggta aatcgtcatt ttgacggcaa
     1261 gcacggcggg gtcggcggcg gcttcgcgca tcatttcgac aacgggattg aaggattggt
     1321 aggggtggtg cagcaggatg ggcgattggc gcaccaaatc gaatatggac gcggttttgc
     1381 ccaaggcttt cagtctgccc ggcgtgtgtg tgggaaattt caaatcgggg cggttgacta
     1441 ggtcggggac ggcgttgagg cgcacgaggt tgaccgggcc tttgacctga tagagttcgg
     1501 cggcggtcag tttgaattgc gcgagcagaa agtcgcggat gtaggcggga caggtgtcgg
     1561 cgacttcgag ccgcacgccg tcgccgtatt cgcggtcgtg cagttcgttt tgaatggcgg
     1621 cgcggaggtt ttgcaggtct tcttcgtcaa cggtcaagtc gctgtcgcgc gtcaggcgga
     1681 actggtggca gcctttgacg ttcatgcccg ggaagagttt gccgacgtgg gcgtggagga
     1741 tggacgacag gaagacgaag ccgtgtccgc cgccacacag ttcggacggc aggggaacga
     1801 cgcgcggcag gatgcgcggt gcctgcacaa tcgccatccc cgaaggcctg ccgaatgcgt
     1861 ccgtgccgtc gagttcgacg gcgaagttga gcgatttgtt cagcggtcgc gggaaggggt
     1921 gggacgggtc gagtccgatg ggggtcagga tcggcagcag ttcgcggtcg aaatagtctt
     1981 cgatccattt tttctgcgcg cccgtccagt tgcggcggcg gtaaaaatgg atgctttccc
     2041 gcgccaactc gggctgaagg acgttgttga acaggtcgta ctggtgccgt atcagggagc
     2101 gcgccgcttc ggtaacgtcg gcgatggttt cagacggcat tttgccgttg tccagcctgc
     2161 gccgggggtg cagcttgttt tcacgcttga gccacgccat gcggacttcg aaaaactcgt
     2221 caagattgga cgacacgatg cacaggaagc gcaggcgttc caaaaggggg acgtttttgt
     2281 cttccgcctg tgccaacacg cggcggttga atgccagcag gcttagttcg cggcagagga
     2341 tgcggttttg ttcgggcata aggttctccc aaagatggtt gttgttcggt cggagcgggc
     2401 aggcaatgcc gtctgaaggc ttggcggcat ctgcgccggt ttcagacggc atgacgtggc
     2461 ggacagtctg tttcaggcat cggggcaatg tgaaaacaga taccggcgcg tgcggtatcc
     2521 gtctgtttca aatcacttcc aatacgaaat aacggcgcag tttttcgtac accgcatcgc
     2581 tgacgttgat gcagccgttg gtcataatcc tgtcggacac ggacggggag gcgatgcgtt
     2641 cgttccgcct ttccgacggt atctgattcc aaacgcggtg cagggcgaaa agaaaatcac
     2701 cctcctgctt gaaaccgatg acttcgccgc cgtatccagg cttgtcggta ctgttcagcg
     2761 tcagcccgaa cgtccccttg ggcgtggcag tgccgatgag gacggggtgg cactgacggt
     2821 cgtcagcgaa acagagttcc gctttggatg tatcgacgat gacttttttc ttttggatat
     2881 aggcattgac cgcatccggc tgcccctgtg catatacggg ggcaaccatc gataggtcgc
     2941 acaatatccc taaaagcagg cgcaacggca tagcggctta ttggcggatg cgtttgggtt
     3001 tggcgggcac gatttcgcgg atgatgactt gcggctcggc tttgggtgcg ggcggcggac
     3061 aggcggcatc tttcgggaag acggggttcc agtagaagct gcgggcaaat ttgtctttat
     3121 cgaaaatcac tttgtattgg caggtagtaa catcttctac gccggaagtg ttttcagggt
     3181 cgatacctac gcccggggta tggaagtgga acaggtaatc ccattcgcgc acgccgtaca
     3241 taccttcgtc gtaatgcggg cggcccagga ttttgtagat gtcgtctttg gtcaggccgg
     3301 ggcgcatctg atccagttcg tcataagtcg ggaatgtgcc gcgcttgttg tcgagcgtta
     3361 cggaataggg tttcgggaaa accggattgt cggtcgtgcc gtcggctttg acgttgcttt
     3421 tggttgtgca ggcagacaga acgcccgccg ccaatactgc caagcccggt ttgacgattt
     3481 gtttgatttt catatgcaga atcctctttt acccgatgcc cgtctgcctt ttgttcggaa
     3541 gagccgcatc aggagagttt taaacatttg attttggttt gtaatattag cataaaaaac
     3601 gtgcgtatca ataaacgcgg tgtatttgta cggcatacgg aataatgcgc gtgcgaattt
     3661 acgcatcctg ccggcaatct gccgattcgc cgcttccggc aacctgttat aattgatgcc
     3721 gactttattt ttccaacgtt tttaagcgaa aacagaatga ctatgcaaga acaatatcaa
     3781 cccgccgcca tcgagcctgc ggcgcagaaa aaatgggacg acgcccgtat tttcaacgtc
     3841 tccgaagacg cttccaaacc caaatattat tgcctctcca tgttccccta tcccagcggc
     3901 aagctgcaca tggggcatgt gcgcaactac accatcggcg atgtattgag ccgcttcaaa
     3961 cttttaaacg gcttcaacgt catgcagcct atgggttggg acgcgttcgg catgccggcg
     4021 gaaaacgcgg cgatgaaaaa caacgtcgcc cctgccgctt ggacgtacga taacatcgaa
     4081 tacatgaaaa cccagctcaa aagcctaggt tttgcgattg actgggcgcg cgaaaccgca
     4141 acctgcaaac ccgaatacta ccgctgggaa caatggctgt ttaccaagct gtttgaaaaa
     4201 ggcatcgttt atcgcaaaaa cggcacggta aactgggacc cggtcgacca aaccgtcctt
     4261 gccaacgagc aagttatcga cggacgcggt tggcgttcgg gcgcattgat cgaaaaacgc
     4321 gaaatcccga tgtattactt caaaatcacc gattacgccg aagagctgct caacgacttg
     4381 gacaaactgg aacactggcc ggaacaagtc aaaaccatgc agcgcaactg gatcggcaaa
     4441 tctcgcggta tgaccgtgcg cttcgccgtt tcagacgaca gcaaacaagg cttggaaggc
     4501 gattacgcga aattcctgca agtttacacc acccgccccg acacgctgat gggcgcgact
     4561 tatgttgccg ttgccgccga gcatccgctg gcaaccgccg cagccgccga caaacccgaa
     4621 ttgcaggcat ttattgccga atgcaaagcc ggttcggttg ccgaagctga tatggcgacg
     4681 atggagaaaa aaggcgtgcc gaccggccgc tacgtcgtca acccgctcaa cggcgacaag
     4741 ctggaagtgt ggattgccaa ctatgtattg tggggctacg gcgacggcgc ggtaatggcg
     4801 gttccggcgc acgacgaacg cgatttcgag ttcgccgcca aatacagcct gccgaaaaaa
     4861 caggtcttac aacgcgtaac aatcggactt tcgccagagc aagaagaagc tatgggtttt
     4921 ggtggcggtt ccggatgtgg tgctggttat ggtgatggta gtggttccga ttcaagagat
     4981 ttgccgttta tgcctaataa atggcaagca tggtatggag tcaaagatga cttcatggtc
     5041 ttaatcaact caggtgaact ggacggcatg aattatcaga cagcatttga tgctgtcgcc
     5101 gctaaattac aagctatcgg agctggcgaa ccgaaaaccc aataccgcct gcgcgactgg
     5161 ggcatttcgc gccaacgcta ctggggctgc ccgattccca tcgtccattg cgaacaatgc
     5221 ggcgacgtac ccgtccctgc cgaccaattg ccggtcgttt tgcctgaaaa cgtcgtaccc
     5281 gacggcatgg gttcgccgct ggcaaaaatg cccgagtttt acgaaaccac ctgcccatgc
     5341 tgcggcggcg cggcaaaacg cgaaaccgac accatggaca ccttcatgga atcaagctgg
     5401 tacttcttcc gctacatgtc gccaaaattt tcagacggca tggtcgatcc tgccgccgcg
     5461 aaatactggg gcgcggtcga ccaatacatc ggcggcatcg aacacgcgat tttgcacctc
     5521 ttgtacgcgc gtttcttcac caaactgatg cgcgacgaag gtttggtcaa tgttgacgaa
     5581 ccgtttgaac gcctgctgac gcaaggcatg gtcgtttgcg aaacctacta ccgcgaaaac
     5641 gacaaaggcg gcaaagattg gatcaacccc gccgatgtcg agctgacttt tgacgacaaa
     5701 ggccgccctg tttccgccgt cctgaaagtc gacggactgc ccgtcgtcat cagcggcacg
     5761 gaaaaaatgt ccaagtccaa aaacaacggc gtcgatccgc aagaactgat taacgcctac
     5821 ggcgcagaca ccgcccgcct gttcatgatg ttcgccgcac cgcccgaaca gtccctcgaa
     5881 tggagcgaca gcggcgtcga aggcgcgcac cgcttcctgc gccgcctgtg gcgtaccgtt
     5941 tacgaatact tgaagcaagg cgaagcagtc aacgcgttcg caggcagcca agacggtttg
     6001 tctaaagaac tcaaagacct gcgccacaaa ctgcacgcga ccatcgccaa agtcagcgac
     6061 gactacggcc gccgccagca gttcaacacc gccatcgccg ccgtgatgga actcctcaac
     6121 caatacgaca aaaccgacac cagcggcgaa caaggccgcg ccgtcgccca agaagtattg
     6181 gaagccgccg tacgcctgct gtggccgatt gtgccgcaca tctgcgaaac cctgtggagc
     6241 gaattgaacg gcgcgaaact gtgggaagca ggctggccgg cagtcgacga agccgccttg
     6301 gtcaaatccg aaatcgaagt catggttcaa gtcaacggca aactgcgcgg caaaatcacc
     6361 gtcgccgccg acgcatccaa agccgacctc gaagccgccg cactcgccac cgaaggcgcg
     6421 gtgaaattca tggaaggcaa gcccgccaag aaaatcatcg tcgttcccgg acgcttggtg
     6481 aatatcgtcg tctaaaccgc ttttaaggtt tagccatacg gataaaggcc gtctgaaact
     6541 tggaaacagg gtttcagacg gcctttttaa ggcagattgg gttgtctgca tgacaaaccc
     6601 cgaataactg aaatctcaaa atccaaaaca tgaatttatt tttcgatacc gaattgggaa
     6661 agcaacaaaa taaagcaacc cacaaaatcc gtgtaatgag cgaggcttgg ctggaaaaaa
     6721 acggctactg tccctgttgc ggaagcaagc cgatgaagag atttgccaat aacaaacctg
     6781 ttgcagacct cttttgccca aattgccacg agcaatatga attaaagagt aaaaatcaaa
     6841 aaaccatagg caacagtgtg cctgacggtg catatcgcac catgttggag cgcatccggt
     6901 cagataccaa ccccaacttt ttctttcttg catataaaaa agcggattac tccatacggc
     6961 aattggtgct tgtacccaaa catttcatca cgccggatat gattattccc agaaacaaag
     7021 gcattaaaaa ccggccgcac cacattatgt gttccatcaa tctcgcccct ttgcctgaaa
     7081 gcggcaaaat attcttaaat agacaattcc cgcattatcg aacccgaaac cgttctgaaa
     7141 aaatggcaat ccaatctgtt tttacgcaac caaaatgcgg agcgcaaagg ctggcttttg
     7201 gctgttatga aatgtatcga ccaacttccc gaagaattca cattgtcgca aatatatgaa
     7261 tttgaaaaca aactatccat ccaatttccc caaaacaacc atatcagaga caaaatccgc
     7321 caacagttgc aaattttgcg cgaccaaaat acgatcgaat ttattggtcg cggactttac
     7381 aaaaaaatcg acaaattgcc cccgacttcc aaggcgtttt aatttcaaat catgatactg
     7441 aattcacaag ggatatttat tcttctgtta ttctgaaaac agttgaagta cagcacaata
     7501 ttgccgcaaa aggcagcagc cgtaagaagg tcggggaatt attggcaatt tatgcgtgat
     7561 ttcgataaac cggctgcctc cgaaggcgca gtgaaattca tagaaggtaa tcctgccaag
     7621 aaaatcatcg ccgtaccggg cagactggcg agcatcgtgg tgtaaatctt tttaaaaaca
     7681 tcaaacaatg ttaagatgcc gtccgaaatc cgttttagat tcagacggca ttttttaaat
     7741 ttaatttttt aaggggtaag cctaatatga aatttccttc cttccttcct ttaaacggat
     7801 agtctgcctg tcggcagtca tttcgatatt gggaggttgt gcggtcgttg ctgatgttta
     7861 cggtcatgat tccgccacaa tgaatgctgt agctgccgaa aattatatga aaacggttga
     7921 gttgaacaag tctgccggca atgtcgatac cacatccaga acagcccgca gggtgcaggc
     7981 agtatttcga cgtatgctgc cttatgccga tgcggcaaat aataccggcc ataggtttga
     8041 ctggaaaatg acggttttca aaaacgatga gctgaacgcg tgggcgatgc ccggcgggaa
     8101 aatggcgttt tatacgggga tagtcgacaa actcaagctg accgatgacg aaattgccgc
     8161 cattatggga catgaaatga cgcacgccct gcatgaacac ggtaaaaata aggtcgggca
     8221 gcaaatcttg accaatacgg cggcgcagat aggcacgcag attatattag acaaaaaacc
     8281 ggatactaat ccggaattgg tcggattggg tatggatatt ttgggaacgt acggtcttac
     8341 cttgccttat agccgcagct tggaagaaga agccgatgag gggggaatga tgttgatggc
     8401 gcaggcaggc tatcatccgg cggccgctgt cagggtttgg gaaaaaatga atcaggaaaa
     8461 cgaccaaaac ggctttattt atgctattac ctctactcat ccgacaaaca atgcccgtat
     8521 agaaaatcta aaacggttgt tgccgacagt tatgccggtt tatgagcaaa gtgtcagaaa
     8581 taaggggcgc gttaataaaa aacgtcggcg ttaagggaag gggatcgaga atttgagccg
     8641 ttgttccaaa atgccgtctg aatccgtttg agattcagac ggcatttaaa cagttctgcg
     8701 ccccgcccgt tttgccttca agtccgcgcc gcttaaatcc aatccccctg caacgggcgc
     8761 aaaaaaatcc ccgtgccttg ctgtcgggct atgccgtctg caccgccgcc gtttcggctt
     8821 ggtttgatgc ggtcggggca tttatgcggc tacgccctga tattcagttc ggcggtcttg
     8881 cctgttttgg caggcatatt cagcagcgcg tctattgtga acttgctgat tttgccgttt
     8941 gccaagtcgg ctacgcaatg gcttcgatac ccttgttttt gacgatggtc agcagcttca
     9001 acgccgcgcc gctcggcttt ttaacgcccc gctcccaagc cgaaacgtga ttttttccca
     9061 cgttgagata gatggcgaaa gcggcttgcg atagtgcctc cttctccctg attgccttga
     9121 tgtctccgcc gctcaacggt ttgatttcgg tcaggcagga cttgtcaaag ccgcgcatgg
     9181 tctttttgtc gattgcgccg atgttgtaag ccccttccat catttcatga atggcggcta
     9241 atgcctcgct tttgtatttc atgattctat ctccgtgata atgccgtctt ctttcaaagc
     9301 cgccagctct gcccgcgtgt atttcagata atatgcggcg gcttttcggt aaacgtccag
     9361 ttctttatcc gaaatgtttt ccctgtcgtt cttggcaaag gcgtaaacaa aaaatgccct
     9421 gtctgcctgt ttgagcagta tcaggctgcg atagccgccg cttctgcctt gtccttgcct
     9481 tgctatgcgc tgcttaatca caccgccgcc taaatctgcg tctatcagcc cgttatccgc
     9541 ccgctctacc gcttccagca gctcggaatc gttgattttg tgcttcttgg caaatttcac
     9601 tatccattgg tttttgaata tccgcattgt gtagccgttg attagtgata cttggaatta
     9661 taccagagac agacaacacg gcaagctgcc ttttttatgc cccgcaattt ctccgtttat
     9721 gccgcctgaa acactggcgg ctaacctgat tattttttaa tcaatcgctt ttaatggctc
     9781 aaattgcgtt tttagcgcgt ttcgctgtct tcctatgccc cccatatata accaagcggt
     9841 ttaaccgcca ttcctgctca aaataggcgc ggttccgtgc gttttggctt ttcattccgc
     9901 tacgctccca ctgccaagac ggtgaaaaac cggaaaaccc caaaagttgt taccgattcg
     9961 ttaccatttg ttaccgcatt tgttaccaat tttttatctt tttcttattt tatagtgaat
    10021 taaatttaaa ccggtac
//
LOCUS       DAWHRS010000069         9943 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWHRS010000069 DAWHRS010000000
VERSION     DAWHRS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.069"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(138..1580)
                     /gene="aldA"
                     /locus_tag="WIB82_001201"
     CDS             complement(138..1580)
                     /gene="aldA"
                     /locus_tag="WIB82_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ3842500.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1615..1815)
                     /locus_tag="WIB82_001202"
     CDS             complement(1615..1815)
                     /locus_tag="WIB82_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842501.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1824..2213)
                     /locus_tag="WIB82_001203"
     CDS             complement(1824..2213)
                     /locus_tag="WIB82_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842502.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2422..3339)
                     /locus_tag="WIB82_001204"
     CDS             complement(2422..3339)
                     /locus_tag="WIB82_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3842503.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLADALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3512..4312
                     /locus_tag="WIB82_001205"
     CDS             3512..4312
                     /locus_tag="WIB82_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3842504.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSVMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4360..5136
                     /gene="mlaE"
                     /locus_tag="WIB82_001206"
     CDS             4360..5136
                     /gene="mlaE"
                     /locus_tag="WIB82_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ3842505.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLSLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5187..5681
                     /gene="mlaD"
                     /locus_tag="WIB82_001207"
     CDS             5187..5681
                     /gene="mlaD"
                     /locus_tag="WIB82_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ3842506.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGNSGKTYTVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5718..6308
                     /locus_tag="WIB82_001208"
     CDS             5718..6308
                     /locus_tag="WIB82_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ3842507.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6365..6643
                     /locus_tag="WIB82_001209"
     CDS             6365..6643
                     /locus_tag="WIB82_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ3842508.1"
                     /translation="MHTELNNGTLHISGDITVKTLTGEAFERFQQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6640..7467
                     /locus_tag="WIB82_001210"
     CDS             6640..7467
                     /locus_tag="WIB82_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ3842509.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7592..7954
                     /locus_tag="WIB82_001211"
     CDS             7592..7954
                     /locus_tag="WIB82_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ3842510.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7951..8337
                     /locus_tag="WIB82_001212"
     CDS             7951..8337
                     /locus_tag="WIB82_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ3842511.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8334..8822
                     /locus_tag="WIB82_001213"
     CDS             8334..8822
                     /locus_tag="WIB82_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ3842512.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8904..9071)
                     /locus_tag="WIB82_001214"
     CDS             complement(8904..9071)
                     /locus_tag="WIB82_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ3842513.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9038..9412)
                     /locus_tag="WIB82_001215"
     CDS             complement(9038..9412)
                     /locus_tag="WIB82_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ3842514.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIVGRRVRGFLARRVSPHIGR
                     GVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRSKN
                     ALRATRKSVRLRWRTMSGRGAG"
     gene            9422..9525
                     /locus_tag="WIB82_001216"
                     /pseudo
     CDS             9422..9525
                     /locus_tag="WIB82_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9650..9865
                     /gene="rpmE"
                     /locus_tag="WIB82_001217"
     CDS             9650..9865
                     /gene="rpmE"
                     /locus_tag="WIB82_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ3842515.1"
                     /translation="MKQGTHPNYHEVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         2335 a         2765 c         2477 g         2366 t
ORIGIN      
        1 cgtttaaatt taatccgcta tacaaagaca aaaacatcga cctcggtcat gactgattgc
       61 cgatgaagca ataaaaatgc cgtctgaacc tgaaaacggg ttcagacggc attttctatc
      121 ggggttttaa agcggcatta aatgtcggtt tccaaataaa cgacttgggt ttgcagatat
      181 tcttccaaac cgtgtttgcc gtccgcgccg ccgataccgg attttttcca accggcgtgg
      241 aaaccctgca tcgcttcaaa gttttcgcgg ttgatgtagg tttcgccgaa ttgcaggcgg
      301 cgggtaacgt agaaggcttc gtttaaatta gtcgtataaa cagaactggt cagaccaaac
      361 tcgcaatcgt tagccagtgc gatgacttgg tcgagcgtgt cgaaagcgga aacgggcagc
      421 acggggccga aggtttcttc tttcataatg tccatactgt tgtcggtgtc ggtcagcagg
      481 gttggctcga agaaatagcc gcgcccttcg gcgcgtttgc cgccgcagac cagtttcgca
      541 ccttgtttga ctgcccgttc cactttttcg gcaacggctt tgacggcgcg ctcttcaatc
      601 agcgggccca tttccagcgc gccggcttcg gcttcggcag ggttgccgta gcgcacgcct
      661 ttcatcgcgg cggtcatttt ttcgataaac gcgtctttca ggctgctgtg gacatagacg
      721 cgctcggcgc agttgcagat ttgaccagtg ttgccgacgc gcgaagccaa gatggatttc
      781 accgccaaat ccaaatccgc gtctttcaaa acgatggcgg gggctttgcc gccgagttcc
      841 agcgaaactt tggtgatgtt ggcggaagcg gcttccatca cttggcggcc tgcttcgacg
      901 gagccggtca ggctgaccat atcgacttgc ggatgggcag acaaggcatt gccgatttcc
      961 gcacccggac cgttcaccac gttgaacacg cctgcgggca atccgaccgc atcgacgatt
     1021 tcggcgaaga tgtggcagtt gatcggggtt acgctgctgg gtttgacaac gatggtgttg
     1081 cccgttacta aggcgggacc cattttgcgg gcaatcagga agaaagggaa gttccacggc
     1141 aaaatgccgg caattacgcc cagcggacgt ttgaacaata aaatattttc acgcgggcgg
     1201 tcgctttgga tgatttcgcc ttcgtaacgg cgcgcccatt cggcttggta atcgagatag
     1261 tcggcggtga acatcacttc tacgcgcgcc aagtctttgg ttttgccgcc ttcggcaacg
     1321 atggtgtcgg tcagctcgtc ggcgcgtccg cgtatgcctt gggcgatttt gcgcaaatac
     1381 gcgccgcgtt cgaccgcagg caggcgttcc caagccggtt gcgccgcgcg tgccgccgcc
     1441 acagctcggt caacgtctgc ttttccgcct ttgggttcgc gggcgatggt ttcttcggtg
     1501 gaagggttca atacgtcgcg ccattcgccg ttgaagtcgt tttcaaagcg tccgttgatg
     1561 tacatggcca attgtttcat ttcgggttct ccagttttgt agtcagatgt agttttagtt
     1621 tattcccaaa taaattgccg tacaagtttc ttttgaaatg tttagacgag aagctactat
     1681 gtctgcatat tgttctgcta atttatattc actcgaatgc aactgccgat tattccaatc
     1741 tgcattcgca tcagtaaaag ccccagcatt gccgcctgtg gcataaccaa tcgctaaact
     1801 gccaacggta tccacaacag gtttcaggct gcctgaattt ctctaataaa tgaaacactt
     1861 attctatcag ccgttaatgt aaccaaacaa ttatcaagat aagagtagtt tactaaatac
     1921 tcatcttctt caaattgtat attttgtagg gttagatgat tattttttaa ttctgccgtt
     1981 atttcatact tgaaactatc atctattttt ctaaatccaa tatttgaagt tgatttatcc
     2041 agtatgtaat cattaaagat ttcataggat aattccactt cataaaattg attttcaaat
     2101 attttgaatg ggcagtatga tataaaacaa tgtagttcat aaccatttat ttcaatcaat
     2161 actgcctctt caatggtgtt atctaacttt ttgattttac atttatataa cattttatct
     2221 tgcctttaga taaattgttt tgttttttgg attgttattc aaagttctcc aaaagtcttt
     2281 tcctgatttt gtatgtatcg tggtaacact accattttca ttggttccaa caaaaataaa
     2341 tttagattga cctttggaat cagtacccaa aaactgaaca tatccagaca tgcggatttc
     2401 gggttttaaa tcaaatgcgg cttaccggtt ttcttttttg gtttccgcag gcgtgctgcc
     2461 gaaatattgc ctgaatgcct gagtaaagct ggaaacgtgg cggtagccgc acaaatacgc
     2521 ggtttcgcct atgcttttgc ccccgttttg caacaggtag agcgcgtgct gcatttgttt
     2581 gtggtgcagc cactcgcttg cggtaatgcc gaaatggtcg cgcatacggc gttgcagcgt
     2641 cctttcgctg atgttcagcg cgtctgccag ccggttgact tggtgtgcgc cgtcggcaaa
     2701 cgcggcattt agggtgcggc tgaagtcttc agacggcata gcgtctgctt ccgccgtttg
     2761 ccccgccgcc ggcccgatgc cgtctgaaac cgtgtcccac aagtccgaca gcagccgcaa
     2821 cacgtccgcc tcgcggcgca atgtttcgcc caaatgcccc tttgggacgg ctttcaggca
     2881 ggatgccgcc aagccgcgca ggttcggggg caaatcccat atcctgaccg gttcgcggta
     2941 aagcaggggt gcgaaacgcg cgtattccgg acgcagcagc cattgttcca tacctttaat
     3001 ggtcattttg accgttttgc cgcctcggta aagatagcgg ctgaacagga cttcttcccc
     3061 gacagcaatt aggacaatct tgccgccgtc cgcatcgatt tggaagcggc agcggttgat
     3121 gccgaagtcc aaactgcctt ccagcaagag cacgaacgac acataaggtt cggcgaggcg
     3181 gctgctgcaa aaatcacacc gtgccgttac tgtgccgccg tggatggaaa tgccgttgga
     3241 caaagtatcg aaacggtagc tgccctccac atagctgccg ccgccgcttt tgaccagatt
     3301 gacgaaccgg ctgctgttca aatgttctgc tttttccata gttttctgat aaaggttatc
     3361 atttgaaaga taacattttt cgccacagca aacaatccgc cgccgacaac ggcattttca
     3421 caacatttac cgtccgtatg ccgtctgaaa attgcccgtc ttcccgataa gccttaaaat
     3481 acacgccgtc aaacctaccg tccgtccgta catgagccca tcccccttta tcgaaatgaa
     3541 agacgtcgct tttgcgtatg gcgaccgccc gattctgaac gacatcaatt tcagcattcc
     3601 gcaaggcaat tttgccgccg tgatgggcgg ttcgggcagc ggcaaaacca cgctgatgcg
     3661 gctgattacg ggtcagatcc gtccgcagtc cgggcaggtt ttgattgaag gacgggattt
     3721 ggcgggcttt tcggctgacg aactctacga acaccgccgc cgcatgggcg tattattcca
     3781 acacggcgcg ctgtttaccg atttgtcggt attcgacaat atcgcttttc cgatgcgcga
     3841 actgacacgc ctgccggaag cggtgattcg agatttggtt ttgttgaaac tgaacgcggt
     3901 cggtttgcgc ggcgtggaaa acctgatgcc gtccgagttg tccggcggga tgtcgcgccg
     3961 cgtcgcgctt gcccgcacga ttgcgctcga ccctgaaatt atgttgtacg acgaaccgtt
     4021 taccggcctc gatccgattt ccttgggcgt gattgcccac ttgatcagcc gcgtcaataa
     4081 ggctttgcgt tcgaccagcg ttatggtaac gcacgacatt gaaaaatctt tggaaatcgt
     4141 cgatcaggtg atttttttgg cgcacggcga aattatgttc tccggctcgc cgcaggaaat
     4201 gcgcgaactg gattcgcctt gggtgcgcca gtttgtcggc gggctggcag acggccccgt
     4261 agcataccgc tatccggcgc aaacgtcgtt gcagcaggat ttgctcgggt aaatgagcag
     4321 gtgccgtctg aaaccgttga actttacata tagaaattta tgaattttat ccgttccgtc
     4381 ggggcgaaaa ccctcggcct tattcaatcc ctcggcagta ttacgctgtt tctgctgaac
     4441 attctggcga agtccggtac ggctttcgtc cgtccgcgcc tgagcgtgcg ccaagtgtat
     4501 tttgccggcg tgctgtcggt gctgattgtt gccgtttcgg ggctgtttgt cggtatggtc
     4561 ttgggtttgc agggctatac gcagttgtcg aaattcaaat ccgccgatat tttgggctat
     4621 atggttgcgg cttcgctgtt gcgcgaactg ggtccggtgt tggcggcgat tctgtttgcc
     4681 agcagcgctg gcggtgcgat gaccagcgaa atcggtttga tgaaaacgac cgaacagctc
     4741 gaagcgatga acgtgatggc ggtaaaccct gtcgcccgcg tggttgcgcc gcgcttttgg
     4801 gcgggcgtgt tttccatgcc gcttttggct tcgattttca acgtggcggg tattttcggc
     4861 gcgtatttgg tcggtgtaag ctggctgagc ttggacagcg gcattttctg gtcgcaaatg
     4921 cagaacaaca tcacgataca ttacgatgta atcaacggtc tgatcaaatc cgccgcgttc
     4981 ggcgtggcgg taacgctgat tgccgtgcat cagggcttcc actgcgtccc gacctcggaa
     5041 ggcattttgc gcgccagcac gcgcacggtg gtttcgtccg ccctgacgat tttggcggtc
     5101 gattttatat tgaccgcgtg gatgtttaca gattgacagg gcgtctgaag acgaaacgcg
     5161 gaacatcgga tattcaagga actttaatga aaaagaacat attggaattt tgggtcggac
     5221 tgttcgtcct gattggcgcg gcggcggttg cctttctcgc tttccgcgtg gcgggcggcg
     5281 cggcgttcgg caattccggc aagacttata ccgtatatgc cgatttcggc gacatcggcg
     5341 gtttgaaggt caatgccccc gtcaaatccg caggcgtatt ggtcgggcgc gtcggcgcta
     5401 tcggacttga cccgaaatcc tatcaggcga gggtgcgcct cgatttggac ggcaagtatc
     5461 agttcagcag cgacgtttcc gcgcaaatcc tgacttcggg acttttgggc gagcagtaca
     5521 tcgggctgca gcagggcggc gacacggaaa accttgctgc cggcgacacc atctccgtaa
     5581 ccagttctgc aatggttctg gaaaacctta tcggcaaatt catgacgagt tttgccgaaa
     5641 aaaatgccga cggcggcaat gcggaaaaag ccgccgaata aacgtatgcc atcatattca
     5701 aactaggaca cttaatcatg aaaaaatcct ccctcatcag cgcattgggc atcggtattt
     5761 tgagcatcgg catggcattt gccgcccctg ccgacgcggt aaaccaaatc cgtcaaaacg
     5821 ccactcaagt attgagcatc ttaaaaagcg gtgatgccaa caccgcccgc caaaaagccg
     5881 aagcctatgc gattccctat ttcgatttcc aacgtatgac cgcattggcg gtcggcaacc
     5941 cttggcgcac cgcgtccgac gcgcaaaaac aagcgttggc caaagaattt caaaccctgc
     6001 tgatccgcac ctattccggc acgatgctga aattaaaaaa cgccaacgtc aacgtcaaag
     6061 acaatcccat cgtcaataaa ggcggcaaag aaatcatcgt ccgcgccgaa gtcggcgtac
     6121 ctgggcaaaa acccgtcaac atggacttca ccacctacca aagcggcggt aaataccgta
     6181 cctacaacgt cgccatcgaa ggcgcgagcc tggttaccgt gtaccgcaac caattcggcg
     6241 aaattatcaa agcgaaaggc gtggacggac tgattgccga gttgaaggct aaaaacggca
     6301 gcaagtaatc cataagccat gccgccagac tgaagatggc atggattttt tcctaccgaa
     6361 cactatgcat acagaactca acaacggaac actgcacatc agcggcgaca tcaccgtcaa
     6421 aaccctgacc ggggaggctt ttgagcgttt ccagcagcaa tgccgtctga aagaaaccat
     6481 tgccgtcgat ttcggcggtg tcaaacgtgc cgattccgcc tgcgtgtcgc tgctgctcga
     6541 agtgctgcgc ggctgcaaag gcagcgtcag gctgaccggc attcccgaat ccgtgcgcgc
     6601 gctgtccgaa ctgtacgaaa tcaaagactg gctgaaatca tgaaaaaaac cgcctatgcc
     6661 ttcctcctgc tgatcgggtt cgcttccgcc cctgcatttg ccgaaacccg ccccgccgac
     6721 ccttatgaag gctacaaccg cgccgttttc aaattcaacg accaagccga ccgctacatt
     6781 ttcgcccctg ccgcgcgcgg ctaccgcaaa gttgcgccga aacccgtccg cgccggcgtg
     6841 tccaattttt ttaataacct gtgcgacgtg gtcagcttcg gcagcaatat cttgcgcttg
     6901 gacatcaaac gcgcaagcga agaccttgtc cgcgtcggca tcaacaccac tttcggtttg
     6961 ggcgggctta tcgacatcgc cggcgcgggc ggcattcccg acaataaaaa caccttgggc
     7021 gacacgtttg cttcgtgggg atggaaaaac agcaattatt tcgtgttgcc cgtcttaggg
     7081 ccgtccaccg tccgcgacgc gctcggcacg ggtattacct ccgtttattc gcccaagaat
     7141 atcgtcttcc gcacccctgt cggacgctgg ggcacgactg ccgtatccgc cgtcagtacg
     7201 cgcgaaggcc tgctcgattt gaccgacagc ctggacgaag ccgccatcga caaatacagc
     7261 tacacgcgcg acctctatat gaaagtccgt gcgcggcaga ccggtgcaac acctgccgaa
     7321 ggtacggaag ataacatcga catcgacgaa ttggtcgaaa gtgccgaaac cggcgcggcg
     7381 gaaactgccg ttcaagaaga ttccgtatcc gaaacacagg cagaagcagc aggggaagcc
     7441 gaaacgcaac ctggaacaca accttaaagc catgccgtct gaaaatccgt gttcggacgg
     7501 cattcccttc tgtcggaacg gacagtccga aaccacagat tgggctataa tctctttttt
     7561 gcgcataccg cgcgacagca aaggaaaaac catgtacgaa gtcaaccgca gcgtattcgt
     7621 cctcatccct ttagaacctt tttggaactg gctgcaaacc ctgccgggca accacctcga
     7681 cggactgact cttgaagaca tccaagccga cgccaattcc taccttgtcc gcccgtgcga
     7741 aaccgccgac gaagtatggg acgaaatcga agcccgtttt gaagacattt tcgccgccga
     7801 acttgccgac tggtgcgaag acgaacgcga gtggcctgcg ctcgatgccg acattttcaa
     7861 cgaatggttc gacatccagc tttctaccgt catcaccgac ctcgaacacg aaccgcttgc
     7921 ccgcgaagcc ttccaaccca tcaacctgaa ctgatgaagc tgaccgtccg caactaccat
     7981 ctcgacggct acggccatgt caacaatgcg cgctacctcg aatttttcga agaagcgcgc
     8041 tgggcgtttt ttgaagaacg cggactgctg cacgagttgg caggcctcat actgattgtc
     8101 gcccgcatcg acatccgata cagccgcccc gccgtcgaag gcgacgtatt gcagttttca
     8161 tgccgcctga aaacccccgg cacgcgccgt attgtgctga cccagacgat tacactgcca
     8221 aacggcaaaa ccgccgcaga agccgacatc accctgatgc ccgtccacgc cgccacacaa
     8281 cgcaccgtca gcctgcccgc cacccttgcc cgcgcattgg aagccttgtc cgaatgaaaa
     8341 aaatactcac cgccgccgtc gtcgcactga tcggcatcct ccttgccatc gtcctcatcc
     8401 ccgacagcaa aaccgcgccc gccttctccc tgcccgacct gcacggaaaa accgtttcca
     8461 acgccgacct gcaaggcaaa gtaaccctga ttaatttttg gtttccctcc tgtccgggtt
     8521 gtgtgagcga aatgcccaaa atcattaaaa cggcaaatga ctataaaaac aaaaacttcc
     8581 aagtccttgc cgtcgcccag cccatcgatc cgatagaaag cgtccgccaa tatgtcaaag
     8641 actacggttt gccgtttacc gtcatgtatg atgcggacaa agctgtcgga caggcgttcg
     8701 gcacacaggt ttatccgact tccgtcctta tcggcaaaaa aggcgaaatc ctcaaaacct
     8761 acgtcggcga acccgatttc ggcaaactct accaagaaat cgataccgcg ctggcacaat
     8821 agcgatgccg tctgaaacgc aaaaaaacag aaacgatgtt cctgtttttt cagatcatcc
     8881 gccccgacac gttcagacgg cattcaacct tccggcagat tctttttcac cactgccgga
     8941 ttgcctgccg ccaaagagta gggcggaatg tcttttgtaa ccaccgcgcc tgcgcccacg
     9001 accgaaccgc gtccgacggt tacgcccgcc attacaatca ccctgcgccc cggccagaca
     9061 tcgtcctcca acgtaatcgg acggatttcc gtgtagccct caaagcgttt tttgaacggt
     9121 caaacttgtg gttatttgaa taaaacagac attccggctc catcatcaca tttttgccga
     9181 ccaccggccc acggcagatt tcacagtttg ccccgatgcc cgagccgtcg cccaaaaccg
     9241 tatccggaaa cacatacgcc ccgcgttcga tattgacccc gcgtccgata tgcggagaaa
     9301 cccgccgcgc caaaaaaccg cgtacccgtc tgccgacgat gccgcgcata tgcgacggcg
     9361 gcaacaccga accggcaaaa cgcagcagca ggaaaaagaa ttttgatccc atcaagcctc
     9421 cctcaaaaga actttttata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     9481 acagtacaga tagtacggca aggcgaggca acgctgtact ggtttttgtt aatccactat
     9541 acattttatc gaatatcctc ttgacgaaac aacagaaaat agcgagaatg ccgagcttgt
     9601 ctgaagcata ggtacatccc ctatgcccgc ttttttgaaa ggaaatcaaa tgaaacaagg
     9661 cactcacccg aattatcacg aagtcaacgt tacctgctcc tgcggcaaca aatttgtaac
     9721 caaatccgcg atggaaaaag aaaacttcaa tatcgaggtt tgctccctgt gccacccgtt
     9781 ctataccggc acccaaaaaa tcgtcgatac caccggccgc gtggacaaat tcaacaacaa
     9841 attcggcaac ctgttcaaac gctgattgcc gctttgcaag aattaagccc tgccatcatt
     9901 tcgatggcag ggctttttaa tatttggcac actgacttct tat
//
LOCUS       DAWHRS010000070         9884 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWHRS010000070 DAWHRS010000000
VERSION     DAWHRS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.070"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(239..967)
                     /gene="rmpM"
                     /locus_tag="WIB82_001218"
     CDS             complement(239..967)
                     /gene="rmpM"
                     /locus_tag="WIB82_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ3842516.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1331..2281
                     /locus_tag="WIB82_001219"
     CDS             1331..2281
                     /locus_tag="WIB82_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ3842517.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2394..3128)
                     /gene="fnr"
                     /locus_tag="WIB82_001220"
     CDS             complement(2394..3128)
                     /gene="fnr"
                     /locus_tag="WIB82_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ3842518.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3333..4754
                     /gene="hemN"
                     /locus_tag="WIB82_001221"
     CDS             3333..4754
                     /gene="hemN"
                     /locus_tag="WIB82_001221"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ3842519.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5107..6681)
                     /locus_tag="WIB82_001222"
     CDS             complement(5107..6681)
                     /locus_tag="WIB82_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ3842520.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            7006..8106
                     /locus_tag="WIB82_001223"
     CDS             7006..8106
                     /locus_tag="WIB82_001223"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ3842521.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGTDELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADTRQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            8226..9167
                     /gene="mafA"
                     /locus_tag="WIB82_001224"
     CDS             8226..9167
                     /gene="mafA"
                     /locus_tag="WIB82_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ3842522.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLITPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKKPDVGNEVIRRRKGG"
     gene            9171..>9884
                     /gene="mafB"
                     /locus_tag="WIB82_001225"
     CDS             9171..>9884
                     /gene="mafB"
                     /locus_tag="WIB82_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ3842523.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNP"
BASE COUNT         2515 a         2933 c         2378 g         2058 t
ORIGIN      
        1 cggcaatggg acacaaacct gaatctgaat ctgaatctga atctgaatcc gaatccgaat
       61 ccgaacctgc accccgattc tgacgatagc cgtttcagaa aaccacccaa aaaaacaaac
      121 cccgcaaaag cgggggtttg tttcacatca tcgatgccga ccgacgtttc acgaatattg
      181 caacagtacc tggtacaaaa tccactaacc tcatgcatcg gcaagatatt gcctagcctt
      241 agtgttggtg atgattgtgt gccggcacaa cctgacgggt tacgatgctg cggattttca
      301 catccacacg gcggtcaggt tcgatacatg caatcagagc ctcacgtttt ttggctttag
      361 agactttcgc acccagtttg gcaacttcgg cttcacaaac ttgagtcatt tgcgcttgag
      421 attcgcccaa gccaacagca gaaattctag aaacaggtac gccgttgctg accaggttgt
      481 ttgccactac gtatgcgcgg cgttcggaca gggcctgatt gtatttgtca gaacccataa
      541 agtcggtatg gccttcgacg cggacagatt ggacattggt tcgactcagg cgttgcgcca
      601 atactttcag gttgtcttga gcttcggcgc gcaatgaatc cttatcgaaa ccgaacaggg
      661 ttttggcaga cagggaaatg gtttcatcaa catattgcgg agcctgctcc acaacgacga
      721 caggcgcggg tgcgggttcg ggttctggct cgggttcggg ggcagcaacc gcatcgccgc
      781 attctacgcg accttggctt gctttatcaa agtaggcgtt tttccagcat tcgccatagt
      841 tgttgcgtac gatttcgttc gactggccgc ttacggtgta accctgaacg gacgcctcgc
      901 ccgcaacagc agtgccggaa gcgagcaatg caacgaataa tgcgcttaat ttcagctgtt
      961 tggtcatttt attccctcat taaatttgta cagcagatac agaagcaact gctgaattgc
     1021 caacatacag gccgctgtag aaacatggcg gcaaatcgga tatttcagtc tccactataa
     1081 agaagcaggg ggaggactgt caatactcaa cgcctgaaaa caaccgtaaa acagcctgtt
     1141 tcctgatagt ctatcatgcc gaaaaatcaa ttccgtgtca ttactttagg gggatttttt
     1201 ccatatcgca cgattgccgt tgcacaaaaa caacaaacac aacggcaaag ccccataccg
     1261 taccggcaag aaaatatgat aaattataaa caatgttcag ccacccgaca cagacccaca
     1321 ccgacccgcc atgaaattac aacaattgaa atacgcctta gaagtttacc agcacaacct
     1381 gaacgtttcc gaagcggccg aagccttgtt tacttcgcaa cccggcatct ccaaacaaat
     1441 caaattgctg gaagaagaaa tcggcattca gatttttatc cgcagcggca agcgcgtggt
     1501 ttcggtctcg cagccgggca aggtggtttt ggatattgcg gaacgtattt tacgcgatgt
     1561 acagaacatt aaaaatatcg gcagcgagtt taccggacag gacagcggtt cgctgacggt
     1621 tgccacgacg catactcagg cacgctatgc cctacccttg attgttgccg attttgtgaa
     1681 acgctatccg aaagtcaacc tgaccatcaa acaggggagc cctgccgcca tcgcacgtat
     1741 ggttacctca ggcgaggcgg acttggcgat tgttacggaa cggatagacg accatcccga
     1801 actgggaaaa cttccctgct atgactggac tcatgcggtt atcgtaccga acgaccaccc
     1861 cttgctcgaa tgcagaaacc ccctccgcat tgaagatttg gcgaggtttc cgctgattac
     1921 ttatgaattt gcattcaatg cgggcagcag catcgcgcgg gcattttcca aagcccgttt
     1981 ggaacagccg gatgtcgcat tggcggcggc ggatacggac gtattgaaga cttatgtgcg
     2041 cttgggtttg ggcgtgggac tgatggcgaa aatggcgtac aacccggata cggacggcga
     2101 tttgcagctt gtggatgcgg cacacctgtt cgagccgtcg ccgacgtgga ttgctttgcg
     2161 cagcgatact tatttgcgcg gatatgccta cgactttatc caagcgtttg cgccgcacct
     2221 gacacgcgag aaggtggata ggattctgta cacgcccatc agcgaggatt tttcgattta
     2281 ggcggctgcc ggttttcaga cggcactttg cggcagatac aacaaacagg acagatgttt
     2341 tcgtctgccc tgtgtttatt gagaatgctg tctgaaatgt tcgtacgggt taatcaaatg
     2401 gcgtgcgagc agccggacac catttttttc aacacctgca gattgaggat tttgatgtgc
     2461 ttatgctcga cggaaatcaa tccttcctga tggaacttgg ataatgtgcg gctgacggtt
     2521 tcaagtttca gcccgagata actgccgatt tcttcacgag acatgcgcag gatgaaatcg
     2581 ttggcagcaa aaccacggga ataaaggcgt tgggagaggt tcagcaggaa ggcggcaatc
     2641 cgctcttcgg cgcgcatatt gcccaacagc agcataacac cttggtcgcg cacgatttca
     2701 cggctcatca tgcggaagaa gtgcgtacgc aggctgggga tgttttgccc cagttcttcg
     2761 atgtgggtaa acggcagttc gcacacttcg ctgtcttcca aggcgaccgc gtcgcaactg
     2821 tgcacatggg aacagatgcc gtccatgccg atgagttcgc ccgacataaa gaaacccgtt
     2881 acctgatcgc ggccgtcctg actggcgacg gttgttttga agaagcccga acggatggca
     2941 aagagcgagg taaaggcttc gccgacacag aacaggtatt cgcccttttt caggcggcgg
     3001 ctttgacgga tgacggcatc gagttggctg agctcgttgg gcagcagccc gacaggcagg
     3061 cagagttccc gcaaagaaca ggaagaacac agcgttttca tctgatgtgt agtattatgc
     3121 gaagccatac cgtacctttt tgtgcgcttt gccccatcat gattatagtg gattaaattt
     3181 aaaccagtac ggcgttgcct cgccttgtcc ttatttaaat ttaatccact atatgtgctt
     3241 attgacacat atcaagacag gtttatcata ctgtggcatt ctaccaaact atccaaaatt
     3301 tgactaacat cataaaatcg cacacacaca ccatgaaaat cattcagata cagaacaatc
     3361 acaatgtaaa cgatgaccgc cccgagtttg accgcgcgct gattgccagc ctgcccgcca
     3421 gcggcccgcg ctacacttcc taccctaccg ccgaccgttt ccatgacggt ttccgcgaag
     3481 gcgaatatat caaagcttta catttgcgcg gtatgggcgc gttaaacaaa ccgctttccc
     3541 tttacattca cattccgttc tgcaacacca tctgctacta ctgcggctgc aacaaaatca
     3601 tcaccaaaga caaaagccgc gccgatgcct acatcgaata tcttgaaaaa gaaatggaac
     3661 tgctcgctcc acatctgaac ggacggcacc agcttgccca actgcacttc ggcggcggca
     3721 cgccgacctt tttgagcgac gaacagatcg aacgtgtctt ccgcatgata cgcaaacatt
     3781 tcgagttaat ccccaccggc gaatactcca tcgaaatcga cccgcgcaaa gtcagccgcg
     3841 acaccgtcct catgctcggc agactcggct tcaaccgcat gagcatcggc attcaggatt
     3901 tcgaccccaa agtgcaggcg gcggtcaacc gcatccaaag ttacgaagaa accaaagaag
     3961 tcatcgatgc ggcgcgcgaa gcggggttca aatccgtcag cgtcgatttg atttacggcc
     4021 tgccgcacca gacttcggaa agcatcaaaa ccaccatcga taccgttttg tcgctcgatc
     4081 ccgaccgcct cgccctttat cactacgccc acctgccgca cgtgttcaaa ccgcaacgcc
     4141 gcatcgatac cgccgccgtt cccgacagcg aagagaagct cgatatgctg caatactgcg
     4201 tccaaaccct aaccgaacgc ggctacgtct tcatcggcat ggatcatttc gccaaacctg
     4261 acgacgaact ctccatcgcc ctcaaagaag gcttcctcca gcgcaacttc caaggctatt
     4321 cgacctacgc ggattgcgat ttggtcgcca tcggcgtgtc gtccatcggc aaaatcggca
     4381 gcacctattc ccaaaacgaa cgcgacatcg atgcctacta tgccgccatc gacgaaggca
     4441 gactgcccat catgcgcggt tatcagctca atcaggacga catcctgcgc cgcaacatca
     4501 ttcaggattt gatgtgccgt ttcgcgctcg actaccggat ttacgaaagt atgttcggca
     4561 tcccgttcga ccgctacttc aaagacgaac tggcggattt ggaaaaactc gccggtttgg
     4621 gattggtgcg cctgaacagc cacgggctga ccgttacccc gaaaggacgc ttcctcatcc
     4681 gcaacatcgc catggtgttc gattaccacc tgcgccataa agaaaccaag gcgaaatatt
     4741 cgcaaacggt gtgattgcgt ctaacgtaca aatgccgtct gaaaggcttt ttcagacggc
     4801 attttgctgc cggcaggata agtgttttca agaacaggcg gcggcatatc ataacgttcc
     4861 gcacctttgt gtccgaccgt tccgaaacca agatatagtg gattaacaaa aaccagtaca
     4921 gcgttgcctc gccttgccgt actagctgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     4981 ttttgttaat cactatacca aacggcatat cccgacagaa cagattgtgc ataaggcaca
     5041 agcccgcaca ttccatcaac aaaatgccgt ctgaacacgg gttcagacgg catcagtatt
     5101 ttacaatcag aatactgcct gtaaaaccaa gtagcagaca accgacaata cggcggcggc
     5161 aggcagggta atgacccacg ccaaaccgat gggcttcatc agtttccagt tggcattgcg
     5221 gttgaccaga ccgatgccga gtaccgcgcc gaccaagata tgcgtactgg acacgggcag
     5281 ccccatcagc gacgcgccca tcacgacgga tgcggcggac aattcggcgg taaaacccga
     5341 ggcggggtgc atttcggcca aactcgtacc gacggtttta atcacctctt taccgacaaa
     5401 ccacaaaccg acaatcagcg cgatgccgaa agtcagcatc gcaatcgggg ggacgacatt
     5461 ttgcgcggca acgctgttgg tacgcaaaac atccataatc gcggcaaacg gaccgatggc
     5521 gttggcgata tcgttcgcac cgtggctgaa tgcgaagccg caggcggtaa agacctgcat
     5581 ccatgaaaac atctgaaagg tcgatttgcc caagtcttta cgcttgaggc ttttggcaaa
     5641 cacaaacgtc cccatccaca ccgccgcgcc tatcataaag atggtcagga agctgttgac
     5701 gttgctcatc cccaaatgca ggtttttcaa gcccttgaaa atcagcatag cggaaatcat
     5761 catcgcgccg aacgaagcga taaagggaat ccaagaatgc agcgctttgt aggaatcgac
     5821 attgttttta cggttgtcga acgcataaag accgcggtaa tactccgatt gcagctcttg
     5881 cggatcgaat tcgggttcgt cgtaaatttg cgcgtcgtgc gccattttgg tggcgtactc
     5941 gactttttcg gcttcggaca aaccctcgaa aaacaggcgg tgccgttctt tataggcctt
     6001 tttttcctgc ttgatgccct tgagcgtgcc ttccgcccaa gcgttgtaat ctaagacgtt
     6061 tttcttgacg cgcgaaaaca ggaaatagga caccgcgccg cccaacacgg gcgacaatac
     6121 ccaagaaaca ccaataccgc ccagcttgcc ccaacgtatc aaatcgcccg atgcggcatc
     6181 gttcattacc gccatacata ccgcgctgcc gacaatgccg ccgataatgg aatgggtggt
     6241 agataccgga agcccttttt tcgaggcaaa caacagccac aacgccgccg ccaaaagcgc
     6301 ggacatcata ataaacacaa actgtatggg ttcgaaatca acacccttca aatcgacgat
     6361 gcctttgcgt atggtattgg ttacctcgcc gcccgcgatg accgcgccgc tgacctcaaa
     6421 taccgccgca atcagcaaag cctgcgggat ggtcagcgta cccgcaccga cgctggtgcc
     6481 gaaagaattg gcaacatcgt tgccgccgat gttgaacgcc ataaacacgc cgaacatcgt
     6541 ggcaataatg aataaggcgg cgtaattgta atgggtataa cccaagcccc aataaataaa
     6601 atagccgact gtacctacca gcatggcggc aaagacggcg ttgatggttt taacatttgc
     6661 gctcatttgg atttgagcca tggcaatctt cctttaatgt ggtggaaatc atgagtgttg
     6721 ccgacactgt actgctgcct ccggtcgaac tgccgtttcg ggatatggca gacgcatcct
     6781 tccttctgaa ccgcatccga atacccccaa acctgaccga atgcaaaact gctgccggaa
     6841 acggtttgta tgacacttct actactggat gttgcgggcg cattataata tttttccatc
     6901 cgtcttgaaa catttattta cactttattt acactgcggc ggcaaatcgg tatacgagcg
     6961 tcaatacacg ttaaaatggc gttttgcacc agtttgggag tgatgatgga aacacagctt
     7021 tacatcggca tcatgtcggg aaccagtatg gatggggcgg atgccgtgct ggtacggatg
     7081 gacggcggca aatggctggg cgcggaaggg cacgccttta ccccctatcc cgacaggctg
     7141 cgccgccaat tgctggattt gcaggacaca ggcacagacg aactgcaccg cagcaggatt
     7201 ttgtcgcaag aactcagccg cctatatgcg caaaccgccg ccgaactgct gtgcagtcaa
     7261 aacctcgcgc cgtccgacat taccgccctc ggctgccacg ggcaaaccgt ccgacatgca
     7321 ccggaacacg gttacagcat acagcttgcc gatttgccgc tgctggcgga acggacgcgg
     7381 atttttaccg tcggcgactt ccgcagccgc gaccttgcgg ccggcggaca aggcgcgcca
     7441 ctcgtccccg cctttcacga agccctgttc cgcgacaaca gggaaacacg cgcggtactg
     7501 aacatcggcg ggattgccaa catcagcgta ctcccccccg acgcacccgc cttcggcttc
     7561 gacacagggc cgggcaatat gctgatggac gcgtggacgc aggcacactg gcagcttcct
     7621 tacgacaaaa acggtgcaaa ggcggcacaa ggcaacatat tgccgcaact gctcgacagg
     7681 ctgctcgccc acccgtattt cgcacgaccc caccccaaaa gcacggggcg cgaactgttt
     7741 gccctaaatt ggctcgaaac ctaccttgac ggcggcgaaa accgatacga cgtattgcgg
     7801 acgctttccc gttttaccgc gcaaaccgtt ttcgacgccg tctcacacgc agcggcagat
     7861 acccgtcaaa tgtatatttg cggcggcggc atccgcaatc ctgttttaat ggcggatttg
     7921 gcagaatgtt tcggcacacg cgtttccctg cacagcaccg ccgaactgaa cctcgatcct
     7981 caatgggtgg aggcagccgc atttgcgtgg ttggcggcgt gttggattaa ccgcattccc
     8041 ggcagcccgc acaaagcaac cggcgcatcc aaaccgtgta ttctgggcgc gggatattat
     8101 tattgagcgc aaataccgtc tgaaagccat ttccgccgct cagacggcat tttcgcccct
     8161 tttgtttaca aacccttaaa atccctttac actcaaaatc cgttcaacat caaacaaacc
     8221 ccgctatgaa aaccctgctc ctcctcatcc ccctcgtcct cacagcctgc ggcacactga
     8281 ccggcatacc cgcccacggc ggcggcaaac gctttgccgt cgaacaagaa ctcgtcgccg
     8341 catcgtcccg cgccgccgtc aaagaaatgg atttgtccgc cctaaaagga cgcaaagccg
     8401 ccctttacgt ctccgttatg ggcgaccaag gttcgggcaa cataagcggc ggacgctact
     8461 ctatcgacgc actgatacgc ggcggctacc acaacaaccc cgaaagtgcc acccaataca
     8521 gctaccccgc ctacgacact accgccacca ccaaatccga cgcgctctcc agcgtaacca
     8581 cttccacatc gcttttgaac gcccccgccg ccgccctgac gaaaaacagc ggacgcaaag
     8641 gcgaacgctc cgccggactg tccgtcaacg gcacgggcga ctaccgcaac gaaaccctgc
     8701 tcgccaaccc ccgcgacgtt tccttcctga ccaacctcat ccaaaccgtc ttctacctgc
     8761 gcggcatcga agtcgtaccg cccgaatacg ccgacaccga cgtattcgta accgtcgacg
     8821 tattcggcac cgtccgcagc cgtaccgaac tgcacctcta caacgccgaa acccttaaag
     8881 cccaaaccaa gctcgaatat ttcgccgttg accgcgacag ccggaaactg ctgattaccc
     8941 ctaaaaccgc cgcctacgaa tcccaatacc aagaacaata cgccctttgg accggccctt
     9001 acaaagtcag caaaaccgtc aaagcctcag accgcctgat ggtcgatttc tccgacatta
     9061 ccccctacgg cgacacaacc gcccaaaacc gtcccgactt caaacaaaac aacggtaaaa
     9121 aacccgatgt cggcaacgaa gtcatccgcc gccgcaaagg aggataaacc gtgaaaccgc
     9181 tgcgcagact gacaaacctc cttgccgcct gcgccgtagc ggcggccgca ctcatacagc
     9241 ccgccctcgc ggcggacttg gcgcaagacc cgttcattac cgataacgcc caacggcagc
     9301 actacgaacc cggcggcaaa taccacctct tcggcgaccc gcgcggcagc gtttccgacc
     9361 gcaccggcaa aatcaacgtc atccaagact atacccacca gatgggcaac ctgctcatcc
     9421 aacaggcaaa catcaacggc acaatcggct accacacccg cttttccgga cacggacacg
     9481 aagaacacgc ccccttcgac aaccacgccg ccgacagcgc gagcgaagaa aaaggcaacg
     9541 ttgacgaagg ctttaccgta taccggctca actgggaagg acacgaacat catcccgccg
     9601 atgcctacga cggcccgaag ggcggcaatt accccaaacc tacgggcgca cgagacgaat
     9661 acacctatca cgtcaacggc acagcccgca gtatcaaact caatccgacc gacacccgca
     9721 gcatccggca acgcatatcc gacaactaca acaacctcgg cagcaatttc tccgaccgcg
     9781 ccgatgaagc caacagaaaa atgttcgagc acaatgccaa gctcgaccgc tggggcaaca
     9841 gcatggagtt tatcaacggc gtcgccgccg gcgcgctcaa cccc
//
LOCUS       DAWHRS010000071         9875 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWHRS010000071 DAWHRS010000000
VERSION     DAWHRS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.071"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(314..547)
                     /locus_tag="WIB82_001226"
     CDS             complement(314..547)
                     /locus_tag="WIB82_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ3842524.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(713..913)
                     /locus_tag="WIB82_001227"
     CDS             complement(713..913)
                     /locus_tag="WIB82_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ3842525.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1085..1960)
                     /gene="xerD"
                     /locus_tag="WIB82_001228"
     CDS             complement(1085..1960)
                     /gene="xerD"
                     /locus_tag="WIB82_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ3842526.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2012..2452)
                     /locus_tag="WIB82_001229"
     CDS             complement(2012..2452)
                     /locus_tag="WIB82_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ3842527.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2876..3814
                     /locus_tag="WIB82_001230"
     CDS             2876..3814
                     /locus_tag="WIB82_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ3842528.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYSSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3942..4235
                     /gene="hfq"
                     /locus_tag="WIB82_001231"
     CDS             3942..4235
                     /gene="hfq"
                     /locus_tag="WIB82_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ3842529.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            4320..4886
                     /locus_tag="WIB82_001232"
     CDS             4320..4886
                     /locus_tag="WIB82_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3842530.1"
                     /translation="MLLKNILPFAHCLLRQALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            4940..5314
                     /locus_tag="WIB82_001233"
     CDS             4940..5314
                     /locus_tag="WIB82_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ3842531.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5324..5818
                     /gene="folK"
                     /locus_tag="WIB82_001234"
     CDS             5324..5818
                     /gene="folK"
                     /locus_tag="WIB82_001234"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ3842532.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPHLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVTELSKQLGNQGIRL
                     LPEE"
     gene            complement(5954..6427)
                     /locus_tag="WIB82_001235"
     CDS             complement(5954..6427)
                     /locus_tag="WIB82_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ3842533.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSAYKRALSALSRKL
                     GRCKDKLHTAVQPVLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAML"
     gene            complement(6647..7384)
                     /gene="ubiE"
                     /locus_tag="WIB82_001236"
     CDS             complement(6647..7384)
                     /gene="ubiE"
                     /locus_tag="WIB82_001236"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ3842534.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKSGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7427..7795)
                     /locus_tag="WIB82_001237"
     CDS             complement(7427..7795)
                     /locus_tag="WIB82_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ3842535.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQLVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7915..9534
                     /gene="mnmC"
                     /locus_tag="WIB82_001238"
     CDS             7915..9534
                     /gene="mnmC"
                     /locus_tag="WIB82_001238"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ3842536.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPTFVRALLSHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGAHSSC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAQLSPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         2661 a         3003 c         1981 g         2230 t
ORIGIN      
        1 tctaccgatt acacaaacac atatcatctt tacacaatca tgcttccatc aacagtaaaa
       61 catgatatga ttgccaacaa taacatctca caataaattt tctaattttt attgaaaaaa
      121 tcaataaata agaattctcc caaccgacaa ataaaataaa gaaagggtta atatgcaaca
      181 tcgaagaaga ctagcaattt accaagcatc caaacgtgct tcctttaccg gcaggtcatc
      241 cgccccgcaa aaacgtaaag aacgttgatt tgaaaaaaat gccgcctgaa gtcctgcttc
      301 agacggcatt tttttaccgt tcgagaaact gtttcaacct gtcctcatcc aaaaaccagt
      361 gacagatttc ctcatctccc gccaacaaaa cgggaaccag ctcattgtat ttttcttcca
      421 aaacaggatt ttcatccaca tcgaccactt ccagcccgaa cccgtattca ttttgaaaag
      481 gtttgagtgc atcgcgcatt ttgtggcaca agctgcaata ttcacgaaac atcaaggtca
      541 atttcatccg cgtttcctta tctgtcaatt tgcacacgcc aaagccttag acgcagcaga
      601 atcatggtct atttgggaaa aaacaatgtt ttcaaggaag atgatactca agttctgcca
      661 aaaacagcaa aaatgccgtc tgaacagttc agacggcatt tcgaaaaccg ttttacgctt
      721 gaacgttgat acccgccgta accgtcggtt gcgcattgtg cgccgtcaga aacgaagcgg
      781 caagctcccc gcagcagccg cagcagctcg ctacgcccaa ttgcgactgc aaatcgccca
      841 ttgtggtcgc gccggcggcg atggtttcct tgatttgatg gtcggtaacg gcattgcaga
      901 tgcagacaaa cattttgtgc tccgtgtgtt ctcaaactat cgtaccgata gcggctttta
      961 ttatcgtatg caaatataaa taaaaacggt tcgcattgca aggtcggcat acacggttta
     1021 tcttggtaat tttttatcaa gtttgtattt tcgattgatt tcttattaaa ccgaagcaga
     1081 agcctcagtt tcgggaatgg tgttccttca ctacaccctg caaccatacg ttggcaacat
     1141 gggtataaat ctgcgtcgta ttcaaatcgg catgtcccaa catatcctga accacgcgca
     1201 aatccaagcc gtgccgcacc agatgcgtgg caaaggcgtg gcgcaggctg tgcgggctga
     1261 tgtgcccgat gcctgcctga cttgcatatt ctttgacaat catccatgcc aactgacgag
     1321 aaatgcccgt ctttttctga ctgacaaaca atgcgtcgca attcctgcct ttcagcagaa
     1381 gtgggcgtgc ctccgtataa tagcgttcca cccaatacgc cgactcctgc cccatcggga
     1441 ccatcctctg cttatcaccc tttcccagcg cggtaataca gcccctgtcc aaatccacat
     1501 tgccgaagtt cagcccgacc gcctcgctga cgcgcaagcc ggtcgcgtac atcaattcga
     1561 gcagtgcctt gtcccgcaaa ccgtgtggcg tatcagtatc cggagtggca agcagccggg
     1621 aaatctgctg ttctgtaatc agtgtcggaa tattcttgtc gattttgggc ggtttcagca
     1681 aacgggtggg attgtccgtc cttatgcctt cacgctccat ccatatatac aggcgtttgc
     1741 acgccgataa tgcgcgcgcc tgcgaactcc gttgctctcc gtcaacataa accgccgccg
     1801 ccaaatccgc ttcgtccgca tccttcagca ttctgcccga ttgggacagg cggcgggcga
     1861 ttttttccaa atcgcgccgg taaccgttta aagtattctg actgagccgc ctgtccaacc
     1921 acagcgtttc aagcagcctg tcgatcaaac cttcttccat accgttccaa acaaatgccg
     1981 tctgaatctt cttcagacgg catggttcac attatcggga aagcgtttcc aatacttcct
     2041 gcgcgtgacc cgccactttg actttccgcc attcatgggc gatttctcca tccttattca
     2101 agacgaacgt actgcgctcg atacctaacg actctttccc gtacagtttc ttcaatttga
     2161 tgacatcaaa caggcggcac actgtttcat ccttgtcgct caacagctcg aaccggaaac
     2221 cctgcttggc gcaaaaattc tgatgcgcct ttacgccgtc gcgggaaata ccgaccacgg
     2281 tataacccaa tgcctcaaac tgttccaaac gcgcattgaa atccaagcct tccgtcgtac
     2341 agccaggcgt actgtctttc ggataaaaat acacgaccaa aggcagatgt tctgccgaat
     2401 gaaaatccgc accgctgctc gaaggcaggg taaattcata tttcacatcc atagtcctac
     2461 tcccgatatt cccattattc aaaacggcac gcagaggcac cgccgcaatt tccaaaccaa
     2521 ccccgattct accgccccaa aggacaagga ttcaaccgcc ggaaacatcc aaaccgacac
     2581 acgacggcat gaaaaatatc catgtcaaac cacaaaatat agtggattaa caaaaatcag
     2641 gacaaggcga cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac
     2701 tggtttttgt taatccacta tatgttccga tttaaaaaca gaatgttata aaacccaatc
     2761 cccaaaacac aacaagaccg cccgcctacg ggcagtctcc tgtcagacga catactttac
     2821 agatggctgt tttttcaaca aaataacgcc aatactcaaa aatatggaat caaaaatgtc
     2881 catccatact ctgaaacgcc tgccctcatc gctgctgctc ggtctctgcc tttccctgcc
     2941 gtcagcccac ctttttgccg acaacgacat tttagggcaa tttttagaac agaacatgct
     3001 tacctcctcc gatccgatag aaatattcgc cgaaagcacg atacacccca ccaacaccca
     3061 agccattaca ggcggtctga ttctctcctc acagtctgcc ctggtcgtca acaacaaaac
     3121 cggacagata ctgtatcaga aaaacgccga caggattatg cccatcgcct ccatttccaa
     3181 actgatgagc gcgatggtcg ttttggatgc aaacttggac atgaacgaaa ccgttaccat
     3241 tacgcccgac gaaatcgacc gcatcaaagg gaccggcagc cgtcttgcca taggtacggc
     3301 acttacacgc aaagaactgc tgcacctgag cctgatgagc agcgaaaacc gcgccaccca
     3361 tgcattgggc agaacctacc ccggcggcat gggcgcattt gtcgccgcta tgaaccgcaa
     3421 agcccaaagc ctgggcatgt acagcagccg cttttacgaa ccgaccggac tcaacttcca
     3481 aaacgtttcc accgccaaag acctgagcct tatggtcaac gccgccgccc aatatccgca
     3541 aatccgcacc aactcgactt ccaactacgc ctcggtacag accaaaaacg ggcagcagaa
     3601 ctacaaaaac tccaatgcgc tggtcaggga aggcatgtgg aacatcgaat tgcagaaaac
     3661 cggctacata cgcgaagcag gcaggtctat ggttgtcaaa gccaacattc aaaaccaacc
     3721 cgttaccatc gtattgctga actctcccac atccgccaca cgcgtcaacg acgcccgcaa
     3781 aatcgaatca tggatgctgc agcaacgctc ctgacataca aatgcccggc ggaaaaccgc
     3841 ccaaacacaa aaaaattggt aatttttcct attcaaggct acaattcgac acgcacaggg
     3901 catctgcccg atttcacaat cataatagcg gagttaaaaa tatgacagct aaaggacaaa
     3961 tgttgcaaga tcccttcctg aacgccctgc gtaaagagca tgttccggtt tcgatttacc
     4021 tggtcaacgg catcaaactg caaggtcagg ttgaatcttt cgaccaatac gtcgttcttc
     4081 tgagaaacac ttccgtcacc caaatggttt acaaacacgc catttccact atcgtaccgg
     4141 ctcgctccgt caacctccaa catgaaaaca aaccccaagc cgcaccggct tcgacccttg
     4201 tccaagtgga aaccgtccag cagcctgccg aataatccgc acgaagcatg acgtgtcata
     4261 tctttcaata ccttaccgga caacggtaag gtatttttat tttcagacgg catttcaaaa
     4321 tgttattgaa aaacatcctt ccattcgccc attgcctttt gcggcaggca cttcccgaag
     4381 gcggcaatgc tttggacggc accgccggca acggacacga cacccttttc ctcgcacaaa
     4441 ccgcaggcat ccgggggaaa gtgtgggcat tcgacatcca gccgcaagcc ctgagccgca
     4501 ctcgatgccg tctgaaagaa gcgagatacg agaatgtgca actcatctta gacggacacg
     4561 aaaacctgaa acaatatgtt tcagaaccac taaatgccgc cattttcaac tttggctggc
     4621 tgcccggcgg agataaaagc cttaccaccc atacggaaac cagcattccc gccctttgcg
     4681 caaccctctc cctgctgaaa gaaggcggta tgcttattgc cgtcctctat ccgggacacg
     4741 aaaacggcaa acaggaggca gaagcaatcg aacaatgggc aaaaaacctg cctcaagaac
     4801 agtttgccgt tttgcgttac ggctttacca accggaaaaa cagcccgccc tatctgttgg
     4861 catttgaaaa gttacgtcaa aaataaatgt ttgcggtaaa ataagccgat tccaaccctt
     4921 attttgataa gagccgacca tgcagtatct gattgtcaaa tacagccacc aaatcttcgt
     4981 taccatcacc attttggtat tcaacatccg tgttttccta ctttggaaaa atccagaaaa
     5041 gcccttggtc ggcttttgga aggcactgcc ccaccttaac gacaccatgc tgctgtttac
     5101 aggattgtgg ctgatgaaaa ttacccattt ctccccgttc aacgcgcctt ggctcggcac
     5161 aaaaatcctg cttctgctcg cctatatcgc attgggtatg atgatgatgc gcgcccgtcc
     5221 gcgttcaacc aagttctaca ccgtttacct gctcgctatg tgttgcgtcg cctgtatcgt
     5281 ttaccttgcc aaaaccaaag tcctgccatt ctgaaacact gccatgaaca acagacattt
     5341 tgccgtcatc gccctgggca gtaatcttga aaaccctgcc caacaggtac gcgccgcatt
     5401 ggacacgctg tcgtcccatc ctgacatccg tcttaaacag gcttcctcac tgtatatgac
     5461 cgcgcccgtc ggttacgaca atcagcccga tttcgtcaat gccgtctgca ccgtttccac
     5521 cactctggac ggcattgccc tgcttgccga actcaaccgt atcgaggctg atttcggacg
     5581 cgaacgcagc ttccgcaacg cgccgcgcac attggatttg gacattatcg actttgacgg
     5641 catctccagc gacgaccccc accttaccct gccgcatccg cgcgcgcacg aacgcagttt
     5701 cgtcatacgt cctttggcag aaatcctccc tgattttatt ttgggaaaac acggaaaggt
     5761 taccgaattg tcaaaacagc tcggcaatca aggtatccgc cttttgcccg aagaataatc
     5821 cgccacacgg aaatgccgtc cgaacaccgg tttcagacgg catttttcct ttgccgccaa
     5881 cacgcgtgca aaaaaaatcg ccccttggga aaggggcgca aaaggaacac aaaccactac
     5941 caaaacttta aatctacaac attgcctgcc gcatactgta tccgacagga tataaagccc
     6001 tcactaaatc gtttcgagaa atccaaactt cttcatcgcc gacagaaaat ctgcctttct
     6061 ccggtaccag ctccaacaga accggttgaa ccgccgtatg caacttgtcc ttacaccgcc
     6121 ccagctttct ggacaacgcg gacagggcgc gtttgtaggc actatccttg cggtcaagct
     6181 cccgcgcact gccgacccag ctcaaacgaa ggttgcggct ttcatcctca atcagcaaaa
     6241 tgcctgtttt aacttccccc acctcgggct cgcatgaaag cgaaatataa tatttgccat
     6301 cgatgttgac atacgccgcc tcatgcacct gacagttgca gccggaagtg gcaagcagcg
     6361 aataaacaga atcccgatgc agtttcaaaa tatcaaggga aatctgttta aaaatacttt
     6421 ccggcatttt tccctccttt cgtctgctgc gttgtttact ggattgactg cattatacag
     6481 acatctccca atcttgcaaa tgataattgt tcctattttt taaaaaattt ataaattatt
     6541 gatggatatg tgaattaatt ttaagattta aaatctcttt ctcaaaaaaa cacaaaaatg
     6601 ccgtctgaaa cgtgttcaga cggcattggc tgcacagcca gtccgtttaa aatttcacgc
     6661 ccttatgtaa cgcaacgatc cccgcactca tattatgata gtccacactg tcaaatccgg
     6721 catccagcat catttgcttt aaagtttcct gatcggggtg catgcggatg gattcggcga
     6781 gatattgata actctccgca tctttggcaa tcagtttgcc catgaccggc agcagcttga
     6841 acgaataaaa atcatacgcg ccttcgagcg gcttgtagat tttggaaaac tccaacacca
     6901 gcaatgtccc gccagatttc aaaacacgat acatctcttt cagcgcggca tctttatgcg
     6961 tcatgttccg caagccgaac gccacggaaa ccaagttgaa ataattgtca gggaaaggca
     7021 gtttttccgc atcggcaagc gacacgggca aaatcatgcc ttcgttcaac agacggtcgc
     7081 gcccgacggt cagcatagag gaattaatat cggtcagcca aacctcgcct tccttgccga
     7141 cccgtttcgc ccaaccgcgc gacaaatcgc ccgtaccacc cgcaatgtcc aacactttat
     7201 cgcctttttt caggtgcgcc gtgttgatgg tgaaatgctt ccacaccctg tgcagccctg
     7261 ccgacatcac atcgttcata atgtcgtagt ttttggcgac ggagtggaac acttccgcca
     7321 cttttccggc tttctcatct tcgttgaccg tgctgaaccc gaaatgcgtt ttctgtccgc
     7381 ccatattcta accacctccc tgatgccgcc ctacggcatc ccaaacttaa caatctgatt
     7441 gcttttcaaa acgggaagca cccgccgccg ccaatttgtc caaatattcc tgccacatcg
     7501 catcgtatcc gtatgccagt ctgtgcagat acgcccaatc gtaaagaccg ctgtcgtgcc
     7561 cgtccgaaaa actgattttc agcgcgtact gtccgacaag ctgcaaatcc gtgatttgga
     7621 catccgcctt gccggtctgc aaaacatcct gtcccacacc gtgtccgcgc acttccgcac
     7681 tcggcgaata gacgcgtaaa aattcggcag gcagattttt aggttcgtcc ccataagtca
     7741 aaaccaacac ggtacggttt ttcaacaaac ggatttcaac aggaatatta ttcaaaaaac
     7801 acctcggttc ggcatacaat atccggcagg cgcaggcgtg ccttcaaacg gcgcgtattt
     7861 taacagcttt atcccgatac cgatattcaa acccacattt cagacggcat tgatatggac
     7921 aatctcgtat gggacggcgt tcccgacatc cgcacactcg accaaaccat ccgcaaacac
     7981 gcacacccgc tcaacctgat tgtctgcctc cccgataatc agattcccga ttttcaaacc
     8041 gcacaagatg cttcggacgc ggaatgccgt ctgaagcacc gtttggatca ggcaacgcag
     8101 tgcctccagt tcgacagcat caacctcatc gaacacatcc tgcccgatgt ccgcttctgg
     8161 ctggttcccc cttcacgcac ccgccgcctg cacgaacact tccaccacat ttcctggcag
     8221 accgaagcca tcccgcaaac cgaaagcaaa cccgacaaac cctggtttgc acttccacaa
     8281 acatccgaac ggcaaaaacc ggaacacatc ctcgttatcg gagcgggcat atccggcgcg
     8341 gcaaccgccc acgccttagc atcacacggc atttccgtta ccgtattgga agcccgaaaa
     8401 gccgcccaag ccgccagcgg caaccgccaa gggctgctct acgccaaaat ctcgccgcac
     8461 gacaccgaac aaaccgaact gctgcttgcc ggctacggct acaccaaacg cctgctcgga
     8521 catatcctgc ccgaatccga aacctggggc ggcaacggca tcatccacct caattacagc
     8581 cgcaccgaac aacaacgcaa tcacgaattg ggtttgcaaa aacaccataa ccacctctac
     8641 cgcagcatca cgcaggcaga agccgaaaaa atcgccggta tcccactgtc cgtcccatac
     8701 gaccaccctt catgcggact ctactggcaa cacggcgtat ggctcaatcc tcccacattc
     8761 gtccgcgccc tcctcagcca tccgctgatc ggactgcacg aagacacacc cttgaccgac
     8821 atttcccacg acggggaaaa gtggattgca agcacgccaa acggcacatt taccgccaca
     8881 cacatcatct actgcaccgg cgcgcacagc tcctgcctgc ccgaaaccaa cctcgccgcc
     8941 ctgcctctca ggcaaatacg cggacaaacc ggcctcacac catccacccc gttttccgaa
     9001 caactgcgtt gcgccgtttc aggcgaaagc tacatcagcc cgtcgtggca cggactgcac
     9061 tgctacggcg cgagttttat tcccaacagc agccataccg gatggaacga agccgaagaa
     9121 gcctcaaacc gccaagcatt ggcacagctt agccccgccc tcgccgaatc attgtttgcc
     9181 gccaacccaa acccccaaaa ataccaaggg cacgccgcca tacgctgcga cagccccgac
     9241 caccttcccc tagtcggcgc actcggcgac attgccgcca tgcggcagac ctacaccaaa
     9301 ctcgcgctgg acaaaaacta ccgcatcgac accccatgcc catacctgcc taatgcctac
     9361 gtcaacaccg cccacggcac acgcgggctt gccaccgccc ccatctgcgc cgccgccgtt
     9421 gcagccgaaa tcctaggctt gccccatccc ctctcaaaac gcctgcgcca cgccctacac
     9481 cccaaccgca ccatcatccg cgccatcgtc agaaggaagg atctaacccc ttaagccttt
     9541 gccgcatgcc gtcccgacca cagaaaatca tattcaaata aacataccaa tcctgacggt
     9601 aaaatatcga aaacacccat aacgttttct tcccaaaata aggagtaagg aatgcctaac
     9661 aaattgcctt tgctgtccaa gatgaatgtt ccgtaacatt cgaagacttc cttaaccctg
     9721 ccagccgttt cggtttctgt accccctgtt agataaggaa ggaatgaaaa agccctgttg
     9781 attgtatgaa gtaaagggcg ttaatttttg ttaatcttcc cttctcagga actcaatata
     9841 taaggatgtt aatttttatt tcccttctcg aggac
//
LOCUS       DAWHRS010000072         9860 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWHRS010000072 DAWHRS010000000
VERSION     DAWHRS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.072"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(137..832)
                     /locus_tag="WIB82_001239"
     CDS             complement(137..832)
                     /locus_tag="WIB82_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ3842537.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPVE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKLLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(947..3058)
                     /locus_tag="WIB82_001240"
     CDS             complement(947..3058)
                     /locus_tag="WIB82_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3842538.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGTVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNLTLNGDYNIGRFENHLTVGMDYSREHRNPTLGFSSAFSASINPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSIDTLSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDPKNNPYIYAVSGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNVTFAAANLFNQKY
                     WRSDSMPGNPRGYTARVNYRF"
     gene            complement(3244..3849)
                     /locus_tag="WIB82_001241"
     CDS             complement(3244..3849)
                     /locus_tag="WIB82_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ3842539.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(3898..4296)
                     /locus_tag="WIB82_001242"
     CDS             complement(3898..4296)
                     /locus_tag="WIB82_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ3842540.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            4404..5315
                     /locus_tag="WIB82_001243"
     CDS             4404..5315
                     /locus_tag="WIB82_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ3842541.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENIV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKASACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            5305..6606
                     /locus_tag="WIB82_001244"
     CDS             5305..6606
                     /locus_tag="WIB82_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ3842542.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKHSPADSDIFATYRQPFPENLSAPQPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDAPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGGLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTYPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(6674..6898)
                     /locus_tag="WIB82_001245"
     CDS             complement(6674..6898)
                     /locus_tag="WIB82_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842543.1"
                     /translation="MVFWLQRLKYPLLLCIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLLAMGTVSAWVAVIWAYLIIESEKNGRY"
     gene            6998..9148
                     /gene="dinG"
                     /locus_tag="WIB82_001246"
     CDS             6998..9148
                     /gene="dinG"
                     /locus_tag="WIB82_001246"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ3842544.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGDPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQNNAQQNLTGFETPEVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQMTGKIAALTDKAELANLADETAASLLDSLHEWQFHLAE
                     EPSLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDSALLDRLTGEFGLFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLILRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            9184..9798
                     /locus_tag="WIB82_001247"
     CDS             9184..9798
                     /locus_tag="WIB82_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ3842545.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT         2324 a         2870 c         2520 g         2146 t
ORIGIN      
        1 ggtttaaatt taatccacta tatattctga tttaaaacca taaggcttta agcaatcatc
       61 tcttctataa agcctaaata caaaaggccg tctgaaatcc tattttcaga cggcctttac
      121 tcattcaatc ctcaacttat tgcggctttt tctgctcttc gcgtacttta tccaccaact
      181 ggtcaatcgt ggtcataccg gactgccagt ctttaaattc aacttggtat ttgccgccga
      241 cgatcacagt cggtgtgccg ctgatttgga atttattggt caactcttcc atttgagccg
      301 cacgcgcttg gctttcagga gcctcaaatg cagccaataa ttttttgccg tcaaacgctg
      361 tttgctcgga cagccatttt ttcagggtat cggtatcggc cagattgatt ttttgattaa
      421 ccatcgcatc gaaaatatgg ctgttggctt tatctgattc accggccatt tccactgcgg
      481 ccgccaaacg tgccaaaggt ttcatttcat caccccacac gacatgctcc cggcgcatat
      541 aggtatcgtc tttaaacgtt ttgatgtgct cgctcaagac cggctcaaga tgggcgcaat
      601 gcgggcagaa gtagccgaaa aattccaata cttcgatttt accggcctgc tgttgcggaa
      661 taggcgtaga caatacagtg tagttcacac cttcgttcaa ctcaacaggg gctgccggag
      721 cagatgagct gctttggacg ctgtctgccg gaacgctggt ttcagcctgt ttgctacaag
      781 cggccaatgc caacagggtc aatgaagtca aagctaaggt tttcagtttc ataggtatct
      841 cttgtgtgtc agattaatgt gcggatttta tgacatttta ttgaaggcgt gttcgatttg
      901 agtgaaaaaa tgtgtttagt tttatttcag cctttgcctg atttcatcag aaacggtaat
      961 ttacccgggc agtatagccg cgcggattac ccggcataga gtccgaacgc caatattttt
     1021 gattgaacag attggctgcg gcaaaggtaa cgttaacatt tttatggttc cagccaagca
     1081 tggcatcaac tcgggcaaag cctggaagcg tagtcacttc tttatttctt gagttgtaac
     1141 cgtagcgttt gcctgtaccg gttacgccga tttcgccgta gaggttttcg gtcggggtat
     1201 aacggaaaaa caggttgccg gtaacgttgc tggtattatt caaatggatg cccactcggt
     1261 cgggattttc tttgtcttca acgactttcg cctgcatcac gcccaacgaa ccgcgcagat
     1321 agagtttttt ggggatgatt tgcccgatgg cggacaattc cacgccgcgc gaacggtgtt
     1381 tgccgctaac cgcataaata taagggttgt tttttggatc ggggcggtag cggatattga
     1441 agcgttcgat ttggtaggca gacaacgtag tgctgaggcg gtcgtccagc caactgcttt
     1501 tcacgccggt ttcgtattgg cgggtgtact cggggtcggc gttgaacacg gcggaagaca
     1561 acgtatcgat gctcaaatag ccgccgcgtc cgccataagg cgcgaagcct ttgttatacg
     1621 aggcgtaaag tgtgtggacg ggattgatgt tccacactgc gccgatgttg gggctgaacg
     1681 agtgtccgct gtattggcgg ctgctgccgg tgagtttgtt ttcggaatta aaggtgtatt
     1741 tgtcgtaacg gccgccgagg acgaatttca aatcgggcgt ggcggagaag atgttttgca
     1801 caaagatgcc gtaggagtcg gctttgtggc ggttttgggt cagaataggc tgcaatctgc
     1861 ccgaagccgg ccagcttgcg cggtcgtagg ggttgatgga ggcggaaaag gcgctgctga
     1921 aacccaatgt cgggttgcgg tgttcgcggc tgtaatccat gcctacggtc aggtggtttt
     1981 caaaacggcc gatgttgtag tcgccgttga gcgttaagtt ggacgacagg gttttgttgt
     2041 cggtctgctg ccaggcgtag ttacgtttga ttaagttgcc attttcgctg cctgcataga
     2101 aatgatcaaa atcctgcgcc gccgtgcggt gggcgagctg ccattgggca cgccatttgt
     2161 cgttgaaggc gtattcaagg tcggaacgcc aaacttgcag cttgtctttg acaaaatcgt
     2221 tccggtgggc gaaccccatg cggtaaggca gtccgaagcg gtcgtacacg gacttggtcg
     2281 gactgcggtc gggcgtgcgc tccacattgt cgtaggtgta ttgccccgtc cacttcaagc
     2341 cgttgtcgag tttgacggta atgctgggcg aaaccatgac atttttgctg tctatgccgc
     2401 tgcggaacga attggcgcgc ccgacttcgc cggtgaggcg gatggcgacg tttttgttca
     2461 gcacttcgtt gatgtccata ttcaggctgc ggtttgccca cgaaccataa accgtaccga
     2521 tattacggct ttgtttgaag ttggcgtatt tgctgaccat gttgatgaca ccgccgccgt
     2581 tggtacgccc ataaagcacg gaggacgggc ctttcaggat ttccacgcgc tcgatgttgg
     2641 cagtactgcg gcgcacttgt ccgctttcgc gcacgccgtc gcggtaaata tcggatgcgt
     2701 cggcttgaaa accgcgcagg aaaatgcttt caccgcgcat atcgtaggca gcgtcgatgc
     2761 cggcattgcc ttcgaggatg gaactcaaat cgttcgtacc gtaatttttg tttttctgga
     2821 tattgagcgt atcgatggtt tgcggcgttt ctttgatgag ctgtccgttg cgggtaacgg
     2881 cggcttcgtc gtagttgatg tagcctttga gtacgctggt gtcggactgt ccgaccacgg
     2941 aaacggtagg tagcgtggcg gtgtaatgtt caccattgtc ctgcgtatcg gcggcagcaa
     3001 cagggaagga agcaataatc agcgtgggta ataaagctaa atgaaatgat attttcattt
     3061 ttatactcaa tttaacaaaa caaccgaatt atattgcctc acggaggaaa tgagaataat
     3121 ttcttttaac tatattgaac atgatatttg taaacaaagg tctcagaatg aggaaaactc
     3181 gccgcctgat actgaaaaat gccgtctgaa cagggttcag acggcatttt tttgaccgcg
     3241 aaattatgcg ccgaacactt tcaaacgttc tgcaactggt tcaaaggtct tttcacctgc
     3301 cgccccttca ataccgccga taaccatttg tgcgcgcaac aaccagtttt cggggatatt
     3361 ccacgctttg gcaatcgccg catcgggcaa gggattgtaa tgttgcaggt ttgcacctac
     3421 gccgaccgcg gcaagtgtcg tccaaacggc atactgcacc atcgcgttcg cctggtccgc
     3481 ccaaacggga aagttggcgg cataagcagg gaactgctcc tgcaaacctt tgacgacatt
     3541 ttgatcttca taaaacaaaa tagttgccgc acccgcctta aacaggttca atttttgcgc
     3601 ggtcggttca aaactgtcgg caggcacgac ggcacgcagc gcgtcttcga caaattgcca
     3661 caccttatca tgctcttcgc caaacagcac gaccacgcgg gcagattggg aattgaacga
     3721 agaaggtgtg tgcaaaacgg cgtgttcgac gatttggaca acttcatctt tgccgacggg
     3781 cagattttta tttaacgaat aaatggaacg gcggctttcg gcagcctgtt gcagagattg
     3841 acgggtcatt tcttttcctc aaaaacattc aacaaaatac actttaggca gtccgattca
     3901 ggctttacgc ttgaaaaaca gcctgtccag cgaccatgct ccgccgcccg ccgccgcgat
     3961 atagaggaat acgaagcaga acagcactgc ggactcgccg ccgttggcaa tcgggaacaa
     4021 agcatttccg gaagcgtgcg ccataaaata ggcaaccgcc atctgaccgg acaaaacaaa
     4081 cgcggcaggg cgcgcaaaca ggcccaacac cagcaaaatg ccgccgacaa tttctaaaat
     4141 accggcaagc agcaacagcc cgccgggcga accgctgccc atttcaatgg ggaaggcgaa
     4201 gattttcgac gtaccgtgca acaaaaacag gtaggcggtt acgatacgca aaacagaaag
     4261 caaaaccggt tggatacggt tgcagcaatc ggacataaaa gctcctttga tatttgtctt
     4321 catttcaggc ggtagagtat agatgtttcc ccttttcaaa tatatactgc ttttattgat
     4381 acaacttttc cacacaagaa aatatggaca ccctgttcag cctcaaggtt ttccgccaag
     4441 tcgtccaaag cggtggcttc acccgcgccg ccgacgcgct cggcatctcc accgcaatgg
     4501 caagcaaaca cgtcagccac ttggaaaaca tcgtccaagc caaactcctg caccgcaaca
     4561 gccgcaacct cagcctgacc ggagccgggg aagaatacta ccggcaatgc agttacgcgc
     4621 tcgacacgct cgacgatgcc gcgcaaaaag ccgccggggg gacggaaaaa ccgcaggggc
     4681 tgctgcgcgt aacgatgccg ctgtggtttg ccggcagcca gatatgcaac tggctggcgg
     4741 aataccgcga acgttatccc gaagtaacat tagaactgat tttggacaac cgccacgtcg
     4801 atttgattgc cgaaggcgtg gatttggcgt tgcgcgtttc ccaaaccctg tccccgtcgc
     4861 tgattgcgcg cccgctggcg gaaatcgaat ttgccctgct cgcctcgccc gatttcctga
     4921 ggcgcaacgg cgtgccggaa acgccggaag aagtggcagg gctgcccgcc gtcctgccga
     4981 cctacaccaa ccagcagaaa ctcgacctca cccgcaaatc ggacggcaaa aaataccggc
     5041 ttgaactgac ccccgtcatc cgtaccgaca acacgctgat gatgcgcgaa atgattaagg
     5101 cgagcgcgtg catcggttat cagccgcttt gggcggcgga acacgatttg cgctgcggca
     5161 cgctggtgag gctgctgccc ggatacgccg tcccgaccga ccggctgaac gccgtttatg
     5221 cggacagggc attcttaagc gcgaaagtcc gtagcttcat cgattttctg aacgaaaaaa
     5281 tcgccagcag gaaaggctgc cgaaatgccg tctgaaaccg cccgccccct ttatgcggac
     5341 acgtgcgccg cacacacgct cgtctggttc cgccaaaacc tccgcatccg cgacaacgcc
     5401 gccttatgcg ccgccgttgc cgaaggtttg cccgttatcg gtatttggat tgacgatgcc
     5461 gaaacagaca accctcgccg cgccgcgttc taccgccaat ccgccgccga actcgcccaa
     5521 gggcttgcag ggcgcggcat cccgctctac acggcggcat cgcctgccga gctcgtccgg
     5581 ctcgccgtcc gcctcaatat ccgcgccgtc atcgccgacg aatcccatac ttttgccgac
     5641 aaactcgccg acaacgccct ttggcatgaa ttggacaaac acggcatcgc gttaaccttc
     5701 gtcaatgacc gcgccgtttt cggcaaaacc gacctgatac ccgacggcgg cacggcacac
     5761 accgatttcg accgctaccg cgaagtatgg ctcgaccgct tttccaagca ctcccccgcc
     5821 gattcggaca tattcgcgac ataccgccaa ccgttccccg aaaacctttc cgccccgcag
     5881 cctgccgcgc tttcagacgg catcttcctg ccgcaaaacg gcggcgaaac ggcggcttgg
     5941 cggcagtggc ggcggtttct cgaacaggcg gattcctact ccgttttaaa ggatttcccc
     6001 tcgcgcaaac acacttcgct gatgggcgcg tatttgagtg ccggctgcat ctcgccgcgc
     6061 ctgctcgcgc gggaaagcct cgaacgccgt ctgaacgcgt gggcggacaa catcatccgc
     6121 cgcgattttt tccttcaact tgccttgcag cacgcggatg atgccccttc agacggcaat
     6181 ccggaacaca ccctgcgcct gacgctttgg cagcagggcc agaccggcat tccgattatc
     6241 gatgccgcga tgcgctgttt gcacaaaacc ggtggcctcc accccgccct gagacgcttg
     6301 agcgcggact ttttctgcca cgttttaaac ctcccccgcc gcgaaggcga gatatggttt
     6361 gcccgacagc tgaccgattt cgatgcagca atcaaccaag gcaactggcg gcttgccgcc
     6421 tcacggcaca cctaccccga cattgccgcc gcctcatacc gtaccgaccc ggacggtact
     6481 tttatcaaac ggcacattcc cgaactcgcc cacctgtccg ccgacaccgt ccacacgcct
     6541 tggcggtttg cctgttcggt cgatacccac ggctatcccg cctatcctgt cgccggtatt
     6601 ccctaagcgg tacggcaacc taaaacaaat gccgtctgaa cttccgtttc agacggcatt
     6661 gcgtccggat gactcaatat cttccgtttt tttcactttc aataatcaga tatgcccaaa
     6721 tcaccgccac ccaagccgac accgttccca tcgccagcaa aaaatgctgc caatccgtca
     6781 tcggcggtac aggacaacgg ccgcattcga tttccgcgcc gcccaacaac cggtacagca
     6841 gcatatccgc aatacaaagc aacaacgggt atttcaaacg ctgcaaccaa aaaaccaacc
     6901 acgtccgcac gatttacctc ccccattccg acaacgatat tgtaccgctt ccgtgcaatg
     6961 ttaaaataat cccacttcta ccgccgtaca aaacgctatg ctcaccgatt tagaaaaaaa
     7021 cgccatccgc gaccattacc aaaatatcgg caaaaatctg cccggtttcc gtccgcgcgc
     7081 ttcgcagcgg gaaatgattg cggcggttgc caacgctttt tcgcggacgt tgacgcgtga
     7141 agaaggcggc gatccaccca agcgcgaggg cgagagcatt gccgtaatcg aaggcccgac
     7201 cggcgtgggc aaatcgcttg cctacctgct ggcgggcggc atcatggcgc aaacacgcgg
     7261 caagcggctg attgtgagca gcgcgacggt tgccttgcag gagcagttgg tagaccgcga
     7321 cctgccgttt ttggtcgaaa aaagcggttt ggaactgagc ttcgcgcttg ccaaagggcg
     7381 cggccgttat ctctgcccct acaaactcta tcaactgacg caaaacaacg cccagcaaaa
     7441 cctgacgggt tttgaaacgc cggaagtgtt gtgggacagc aaacccaagc ccgaagaatt
     7501 gaagctgctg cgcgacatcg ccgacgaatt ttccgcccga cggttcaacg gcgaccgcga
     7561 cacttggccg gaaaaaatcg atgacgcgat ttggctcaaa gtgaccaacg accgccacgg
     7621 ctgcctgaaa accgcctgtc ccaaccgtcc ggaatgccct ttttacctgg cacgcgatgt
     7681 cttggaaacc gtcgatgtcg tcgttgccaa ccacgatctt ctgcttgccg acatcagcat
     7741 gggcggcggt gtaattctgc ccgcgcccga aaacagtttc tattgtatcg acgaagcgca
     7801 ccacctgccc aaaaaagccc tcagccgttt tgccgccgaa cattcatgga atattgccgt
     7861 ttggacgctg gaaaaactgc cgcagatgac cggcaaaatt gccgcgctga ccgataaagc
     7921 cgaacttgcc aaccttgccg acgaaaccgc cgcatcattg ctcgacagcc tgcacgaatg
     7981 gcagttccat ttggcggaag agccgtcttt aagtttgggg gtgtctgaaa acgacagacg
     8041 aaccaacagc gaaccgactt ggctgtggga agacggcaaa atccccgaag gcctcgaaac
     8101 caccgtttcc aacaccgcca ttgccgcacg cagcctgctc aaacacgtta tcgggctgaa
     8161 cgatgcgctt tccgctgcgc gccgcgaaaa agaacaagac agcgcgctcc tcgaccgcct
     8221 gaccggcgag ttcggtcttt ttatcgcccg tatcgaacaa atcagcgcgg tttgggattt
     8281 gctctccacc gtccctctcg aaggtgaaga accgctggca aaatggataa cccgccgcgc
     8341 cgacgacaaa aacgactaca ttttcaacgc cagccccatc agcagcgcgt cccacctcgc
     8401 caacagtctg tggcggcgcg cggcaggcgc ggtgttgacc tccgccaccc tgcaatcctt
     8461 gggcaacttt aacctgatat tgcgccaaac cgggctgcaa tggctgcccg aaaccaccac
     8521 cctcgccctc aaaagcccct ttgactttga aaaacagggc gaactctaca tccctcccat
     8581 atacgccagc cccaaagacc ccgaagccca caccgccgcc atcatcgaat ggctgcccaa
     8641 gcttatttcg cccaccgaag ccatcggcac gctcgtcttg ttttcctcgc gcaaacaaat
     8701 gcaggatgtc gccctgcgcc tgcccggaga ctacctgccg ctcttgctcg tacaaggcga
     8761 attacccaaa gccgtcctcc tgcaaaaaca ccaccgggcc atagaagaag gcaaagccag
     8821 catcatcttc ggactcgaca gctttgccga aggactcgac ctgcccggca ccgcctgcgt
     8881 gcaagtcatc atcgccaaac ttcccttcgc catgcccgac aaccccatcg aaaaaaccca
     8941 aaaccgctgg atagaacagc gcggcggcaa ccctttcatc gaaatcaccg tccccgaagc
     9001 cggcatcaaa ctgatccagg ccgtcggccg cctcatccgc accgaacaag actacggccg
     9061 cgtaaccatc ctcgacaacc gcatcaaaac gcagcggtac ggccaacaat tattggccgg
     9121 cctgccaccg tttaaaagga tagggtaaac ataccgccct tcccgcaaca gaaaggaaaa
     9181 atcatgaact tcacccgact gctcaaccaa gtcttaagca cggttcaaaa aaaaggcaac
     9241 acattctccg acagcccgct caattcattc ggcggaggcg cgctggttgc cggtgtcgcc
     9301 tccatgctgc tgaacggtaa aaaccgcaaa accatcacca aaatcggttc gaccgccgct
     9361 ttgggctacc tcgcctaccg gggctatcag atgtggcagc aaaacaaagg gcgggcaacc
     9421 gtaacacaaa gcgatttcca acctgccgga gaaactgaag aaacatacag ccgcaccgta
     9481 ttgcgcacca tgatagccgc cgccgcttca gacggcatga tagacgaagc cgaacgccgg
     9541 actatcgaac aggaaagcgg cacagacccc gaaactgccg catggctcgc cgccgaatac
     9601 cgcctgcccg caagcatcgg ggacatcgcc gccgccgtcg gcaacgatga ggcgttggcg
     9661 gcggaggcct atctggcggc aaggttggtc tgtgccgatt tgtcgcggaa agaaaccgtc
     9721 ttcctcgccc gcctgtcgca ggctttgaaa ctggatgaca atctggtgga gagtttggaa
     9781 aggcaattgg ggttttgatg acacagccgg ggagggaaaa tcatcaaaaa acggttattc
     9841 cgaccccggc ctcaaaacgg
//
LOCUS       DAWHRS010000073         9794 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWHRS010000073 DAWHRS010000000
VERSION     DAWHRS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.073"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(9..1304)
                     /locus_tag="WIB82_001248"
     CDS             complement(9..1304)
                     /locus_tag="WIB82_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ3842546.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLERAQWFW
                     QQSLQAVELVDERVRKHAVDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLSPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDHEFIDDPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1430..2317)
                     /locus_tag="WIB82_001249"
     CDS             complement(1430..2317)
                     /locus_tag="WIB82_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ3842547.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGACPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAH"
     gene            complement(2317..3282)
                     /locus_tag="WIB82_001250"
     CDS             complement(2317..3282)
                     /locus_tag="WIB82_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ3842548.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3298..4422)
                     /gene="potA"
                     /locus_tag="WIB82_001251"
     CDS             complement(3298..4422)
                     /gene="potA"
                     /locus_tag="WIB82_001251"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ3842549.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4617..4724)
                     /locus_tag="WIB82_001252"
                     /pseudo
     CDS             complement(4617..4724)
                     /locus_tag="WIB82_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="WIB82_001253"
     CDS             complement(4803..5072)
                     /gene="rpsO"
                     /locus_tag="WIB82_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ3842550.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5295..6230)
                     /gene="secF"
                     /locus_tag="WIB82_001254"
     CDS             complement(5295..6230)
                     /gene="secF"
                     /locus_tag="WIB82_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ3842551.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6234..8087)
                     /gene="secD"
                     /locus_tag="WIB82_001255"
     CDS             complement(6234..8087)
                     /gene="secD"
                     /locus_tag="WIB82_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ3842552.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="WIB82_001256"
     CDS             complement(8299..8565)
                     /gene="yajC"
                     /locus_tag="WIB82_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ3842553.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8693..>9794
                     /locus_tag="WIB82_001257"
     CDS             8693..>9794
                     /locus_tag="WIB82_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ3842554.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCQPQKGKIFRL
                     DDDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2508 a         2602 c         2435 g         2249 t
ORIGIN      
        1 tgcgacgctt aacggctgtc tttccactga taatatttcg agccgaggaa agaaccgagt
       61 ttgcgcaaaa acggttgcag gataatattc ggctggcgca accgctcaaa cggcttcaga
      121 cggcattcgt cccctaaaat tgcttcggca accgccagac ctgcaatgcc tgttatcgcc
      181 atcccgtgtc cggaataacc ttgcgcataa aaaacattcg cggccaaacg tccgaaatgc
      241 gggacaaggt tggcggtaat gtcgcactcc ccgccccacg aatattcgat tctaacatcg
      301 gcaagctgcg gaaaaacttt aagcatatct tggcggacaa gttcggtcat acgcgcggga
      361 tcgtcgataa attcatggtc tttaccgccg aaaagcaatc tgccgtccgc actgaggcgg
      421 taatagtcca aaatatggcg gttgtcgcat actgccatat tgttgcggat aagccctttt
      481 gcgcgcgccc ccaagggttc ggtggcaata ataaaggtgc tgacggcaat cgccttgcgt
      541 tccaaaggct taaatatcgg actcaaaccg gcataggtat tgacggcgta caccagattt
      601 ttgcattcga cgctgccttc gggcgtgtaa accagccaac cgttttgatg cggttcgatg
      661 cgcgtcatcg gggattgctc gaaaatctgc gcaccggctt cggcagcggc acgagcgatg
      721 cccaaagtgt aattgagcgg atgcaggtgt ccggataagg ggtcgaattg tgccccttgg
      781 tacatatcgc tgtcaagctg ctgtttcaac tcggctttat cccaaagttg ataatgactc
      841 gcaccgtaat gccgttgggc gtgttcatgc cactgctgca actcttccca atgctgcgga
      901 cggacggcaa ccgtggcata accgcgctgc caatcacaat cgacggcatg tttgcggacg
      961 cgttcgtcca ccagttcgac cgcctgcaaa gactgttgcc aaaaccattg cgcccgctcc
     1021 aaaccaacct gtttttcaat ttcctccata ccgcaggcgt agtcgctgat aacctgaccg
     1081 ccgctcctgc cggacgcgcc gaagccgata cgcgcggctt ccaacacaac cgtttcatgt
     1141 ccctgctccg ccaagggcaa tgcagtgcac aaaccactca atccgccgcc gatgatacag
     1201 gtatcggttt tcagacggca ttgaagtttc ggataaacag tatgaggatt aaccgaacta
     1261 aaataataag aaggcagata ttcttgaaaa tcaggacgaa tcattatgtt tactttatca
     1321 ggtgtatttt cagacggcat gatatggttt gccgtaccat gccgtctgaa cgggaaatta
     1381 ctttaagaaa acaggctgac ctgataatgc ggtcagcctg ccctgtcggt caatgcgctt
     1441 tctcagcagc caatttttct tggcggtagg cttctgccgc ttctcggtcg cgcttggttg
     1501 cctgcctcat catccaataa ttgacgatga tgaccaatgt tccgatgatg ccgattagga
     1561 tggtcgccaa gacattcatc tgaggatcaa gacccagctt gattttggag aaaatcacct
     1621 gcggcaatgt ggatgaaccg gggccggaga ggaatgaggt aattaccaaa tcatccaaag
     1681 acagggtaat gccgagcaga aagcctgaag cgatggcagg ggcaatcaaa ggcaaagtga
     1741 tgacaaaaaa gattttcagc ggacacgcgc ccaaatccat tgcggcttct tcgagcgatt
     1801 ggtcaagctc aaccagacgc gaacggataa caacggtaat gtacgccata cacagcgtcg
     1861 tatgtccgag gaagatggtg aaaaagccac gatcgaagta gagatgttgt aaccattcgc
     1921 tgccctgcaa aaatatctgt acctgaataa tcagcagcag catagacaga ccggtaatca
     1981 cgtcgggcat caccataggt gcggaaatca tgccagcgaa caaggtactg ccgcgaaaac
     2041 gtttaatccg cgccatcgca tagcctgcca gcgtgcccaa aacgacggcg gcaagcgaag
     2101 acacaacggc aatccgcagc gacagccaag cggcttccaa gatggtgtcg ttttccagca
     2161 atgcgccgta ccacttggtc gaaaagccgc cccaaacggt taccagcttg gattcgttaa
     2221 acgaatagat gaccaaaaca accagcggga tatacagaaa cgccagcgac agtgccaaca
     2281 tcagtttcaa gaaccaagat aatttggatt tctgcattat ttggctcctt cttccaattc
     2341 gcggttttca taatgctgaa acagggcaat cggcacgacc agcagcgcga ccatcacgac
     2401 ggcgacggcg gaagccagcg gccagttgtt ttgatcgaag aacgcctgcc acaagacttt
     2461 accaatcatc aggttttccg aaccgccgac cagctcggga atgacgaact cgccgacagc
     2521 agggacgaaa accagcatgg agcctgcaat aatgccggtt ttcgacaaag gcagggtaat
     2581 cgtcaagaac gatttgaccg gccccgcgcc caaatcggaa gccgcttcaa gcaggcggtt
     2641 gtcgagtttc accagttgcg tgtatagcgg cagaatcata aacggcagat aggcgtaaac
     2701 catcaccaaa ttgagcgaaa aggcattgta gaacaaatcc aaaggctcgc tgataatacc
     2761 cattttaatc aacaggttgt ttacaatgcc gttatgcccg agcagaccca tccacgcata
     2821 gacgcgcaac aggaacgatg tccaaaaggg cagcataatg gcaagcagca aaccattgcg
     2881 gacagaagga ttggcacgag aaatcgcata ggcggtcgga taaccgacca acagacaaat
     2941 taccgtcgta gtcagcgcag tcttaattga agaccaataa gtcatcagat agatattgct
     3001 gttttcaccg tcgccgaacg gattgagcgt actccaaaaa ttttggaaga tgtctgcata
     3061 attttggtag ctgacagcaa tattcagacg acccaaatcc tcatctatcg tcgttaaagg
     3121 agtaaacggc gggatggcga tttcttgttc ggcaaagctg attttcagca cgatggcgaa
     3181 cggaatcaga aacagcacca aaagccaaat atacggtacg gcaatcaccg cacgctgccc
     3241 cggacggcgg aacagtttgt ttttcagttt attaaggttc attgcattcc ccttaaatca
     3301 acggaacaac ggagtcggtt ggttttccgg ccagctgata tagacggttt cgtcccaagt
     3361 cggcggtgta atgttgcgca cataccagta aggggcgggg acttggcttt tgacgacgcg
     3421 cccgttgccg agcttgatat ggtaaatggc gaagctgccc aaataggcga tttcttttac
     3481 cgtgcctttc gcccagttgt agtcgcccaa atattcgggt ttttctttat ataaatcaat
     3541 atcctctggt cgaatactaa cccaaaggtc ctgctcgctc ggaccaccca aaccgtgatc
     3601 gatgcggacg tggttttcca aaccttcgca ttcgataacg gcatagtcgg catgatcttc
     3661 aatcaccaca ccgtcaaaga tgttggtttc gccgataaac tcggcagtga agcggctgtt
     3721 gggatagtcg tacacgtcgc tgggtgtgcc gacttgctgc aactgaccgt cagacataat
     3781 ggcgatgcgg gtcgccatcg tcatcgcctc ttcttggtcg tgcgtaacca taatacaggt
     3841 tacgccgact tgttccagcg tattgaccaa ctcaagctgg gtttgttggc gcagtttttt
     3901 gtccaatgca ccgaggggct catccagcag tagaattttc ggacgttttg ccagactgcg
     3961 tgccaaagca atgcgctgct gctgaccgcc ggacaattgg tgcggtttgc gtttagcaaa
     4021 tttggtcatc tgaaccaggc ggagcatttc ttcgacgcgc gcggcgattt cgcctttagg
     4081 cattttgtcc tgtttcagac cgaaggcaat gttttgttct acggtcatat gcggaaaaag
     4141 cgcgtaactt tggaacatca tattgatggg gcgatcatag ggtgcaagtt tggtaatatc
     4201 ctgaccatca aggataattt ttccctgatt gggactttcc atacccgcca gcatacgcag
     4261 cagtgtagat tttccgctgc cggaactgcc caaaagggcg aagatttcgt gttgataaat
     4321 gtccaagtcg atgttatcga cagcgtaatt gtcaccaaac tttttcacca aaccttggat
     4381 tttgagataa ggtttggctg aagacgcagt ggttgcggtc ataatggcaa tactccaata
     4441 aaaagacgag taccggcaaa acggattttc gaatgggtga taaaaagctg tttgattgct
     4501 ggcgggagtt aaacgtttga tgccgtctga aactcttgta aagcgcacgg gcagcatgaa
     4561 atggaacaag attccaaaga actttatatt atattagttt atgcggtttt cgggcaatat
     4621 agtggattaa atttaaacca gtacagcgtt gccttgcctt gccgtactat ttgtactgtc
     4681 tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac tataaatatg gatttgagct
     4741 tgtcggaaag caacagaaaa gaaaacaccg cccatttttc tgggcggtgt cggaaagcgt
     4801 aattatttac gcagacccaa gcgggtaatc aacgcgcgat acgtatcggg ctgggtacgg
     4861 cgcaagtagg ccagcaggcg gcggcgttgg ctgaccattt tcaacaggcc gcgacggctg
     4921 tggtggtctt tggggttggc tttgaagtgg ggggtcaggt cgttgatgcg gaaagtcaac
     4981 agagcgactt gtacttcgga agagccggtg tcgccttctt tgcgttggaa atctttaacg
     5041 atttgtgctt tttgttctac ggtcagtgcc ataatgaaaa ctccaaaaat ataagaatcc
     5101 ccatagggat tcagacaagt ttgccaagcc tgaagacaac ggcaaactcc ctatgcccaa
     5161 gataggacaa cgtggcatta tgacacaatt cccgcatttc tgcacaatat tttaagaccg
     5221 tggaagtaac cgttaaaaat gccgtctgaa gcattgtctg cttgagacgg caatgttcag
     5281 acggcatatg cgcttcaaac cacgatttct tctttcttct tcggttcttt accgatattg
     5341 tcgcggctca aaccgaacat tagcagaagc gggctggcaa ccaatacgga agaataaatg
     5401 ccgaacacga tgccaatggt caacgccata gaaaagccgt gcaaggccgc accgccgaac
     5461 accagcatgg atacgaccat cgcctcggtc gaaccgtggg taatgatggt gcggctcatc
     5521 gttgcggtaa tcgcgttgtc gatgacttcc ggcacggcat gtccgcgcat cgccggcttg
     5581 cggaagtttt cacggatacg gtcgaagacg acgacggatt cgttcacaga atagcccaat
     5641 acggcaagga tacccgccaa gacggtcagc gaaaattccc attggaagaa ggcaaagcag
     5701 ccgagaataa tcacgatgtc gtgcatattg gcgataatgg cagatacggc aaaacgccat
     5761 tcaaaacgca tcgacaggta aataatgatg ccgataacga caaaacctaa agccatcaat
     5821 ccattactta ccaattcctc accgacttgc gggccgataa attcgacttg gcgcaaggta
     5881 acgtcgggac tgtctttttt cagcaaatcc ataacctgat tggacaactg tgcggaagta
     5941 acaccttctt tgttcggcag gcggatcatg atgtgtttgt tcgtacccaa tgcctgaacc
     6001 tgtacatcac ctattttcag cgtatcgagg cgttcgcgca tcttattgac atccgcaccc
     6061 tgctgatatt ggacttccat taccgtaccg ccggtaaatt cgacagagaa attcagacct
     6121 ctggtaacca aaaagaacac ggcagcgata aacgtaacca acgaaatgaa ggtcgtcagt
     6181 ttgccgtagc tcataaacgg aatatcgcgt ttgattttaa agagttccat agcttactcc
     6241 ttacctgccg cagtttcggc ttcaggtttc cataccgcac cgatggaaat attctgcaat
     6301 ttgcgtctgc gtccgtacca cagattgacc aacgcacgga atacgacgac ggaggaatac
     6361 atcgaagtca gaatacccaa acagtgtacg accgcaaaac cgcgtaccgg gccggaaccg
     6421 aataccaaaa gcgcgatacc ggcaatcagc gaagtcaggt tggaatcgac aatggtcgcc
     6481 catgcgtgtt ggaaaccgag attgattgcc tgctgcggcg gcacgccggc acgcaattct
     6541 tcgcggatac gttcgttaat caagacgttg gagtcgattg ccatacccaa agtcaacgcc
     6601 agcgcggcca tacccggtaa cgtcaacgtt gcctgcatgg cagacaaaat accgattagg
     6661 aacagtatgt tggcactcaa tgcaatggta gaaaagaaac ccatcagacg atagtaaacc
     6721 accatgaatg cagcaacgat ggcaaaaccc cataaagtcg aatggaagcc tttttcgatg
     6781 ttctccttac ccaaagacgg accgatggta cgttcttcga caatctgcat cggcgcggca
     6841 agagaaccgg cacgcaacag caaagacgta tcattggctt cggctgtcgt catgcttccg
     6901 gaaatttcca cgcgtccgcc ggtaatggca gtacggataa ccggcgcggt tacaacctcg
     6961 gattttcctt ggtcgatcaa aaccatcgcc atgcgtttgc cgacatttgc ggcagtcagt
     7021 tcgccaaaaa tgctgccacc cgcgctgtcc aagctcagac tgacggcagg tgcgcccatt
     7081 tggtcgaaac tcggttgcgc atcgttgatg ttgtcgcccg tcagctcgac ctgtttgctg
     7141 attagcagag tttcgggacg atctccgccg cttgaaagca gctcataacc gctcggcacg
     7201 ttgccttcca atgcctcgcg caacttggca ggatcgtcct ccaccatacg caattccaaa
     7261 gtcgcggtac ggccgatgat gtcttttgcc ttggcagtat cctgaacgcc cggaagctgc
     7321 acgacgatac ggtcggcacc ggactgctgg atgacgggtt cggccacgcc caactcgttc
     7381 acacggttgt gcagggtagt gatgttctgt ttgaccgcat cggaacgcac tttattgacc
     7441 gcctcttccg aaagcgtcaa gacgatattg ctgccgtctg aattcagcgt tgcttcagga
     7501 aacagcttgc gcaactgcgg cagagccttt tgcacatcac ctgcatcctg caaagggacg
     7561 gtcaggctgt ttccagcctg acgcaccgtg ccgctgcgga ttttttcgcg gcgcagttcg
     7621 cggcggatgt cgcccgaata acgttcaaac gttttctgca tcgccgcttt catatcgacc
     7681 tgcatggtga aatgcacgcc gccgcgcaag tccaaaccca aaaacatcgg attggctttg
     7741 attttcgcca tccattcggg gctgtccgcc aacaggttga gcgcggtaat atacccttcg
     7801 cccaaagtgt tttcgatgac gtcgcgcgct ttaagctgcg tttcggtgtc tttgaaacgc
     7861 actttcagtg aattgtccac aacaaacatc ccgtcggtct gaatacccgc gttttgcagc
     7921 gcggcatcca ctttgaattg agtctgttcg ttgatgatga tggcttgtcg gttggtcgat
     7981 acctgcacgg cgggtgtttc gccgaatagg ttgggcagcg aatacactgc ggcaaccgca
     8041 atcgtgaaca caatcagcag atatttccat aaaggataac ggttcatcat tgttccttaa
     8101 tggttggaac cccacccttt cggtggtgtc ggaatcgggc tatttcagaa gaggcaaaaa
     8161 cccttcccag ccaggcaaga ccggaaggcg gcatcttgaa tatgccgccc tgcgtgtcgg
     8221 aacatggtca agccttcggt tggaattcaa aacaaagtgc cgcattcggg cttttccgat
     8281 gcggcttgtc ggcacaaatc aatcgacttt tgcagaaatc gcattgcgtt ccacttcgac
     8341 ctcgattttt gtaccctgtc cgatatccac ggtaaaaaac tgttcgccga ctctggttac
     8401 cttacccttg aaacctgccg ccaagaccac tttgtcgccg actttcaagg cggcaagcat
     8461 tgcctgatgc gctttgaatt tcttttgctg cggacgcatg atcaggaagt agaacaccac
     8521 cataatcaac actaaaggag caaattgtgc aacagcttga ttcataattt atccgttctt
     8581 tctaatatgg ttgaaaatcg agaggggtat ataataacat aagaccgtaa acaatatatc
     8641 gggtttgccg tccgtaccga ccgtataccg cagcctgccc gtccacaaac ccatgtcctt
     8701 gacccgccta atcttgaaat tctatgcact gttgcgcctt tttttgggca aaaacgcccg
     8761 caccgcatgg atttcgcatc ccgcctgtgc cgggcacgaa cccggcgcaa accatcccga
     8821 ttcgcccgac cgcatcctct gcatcgagca ggcattgcgc cgcgccggta tttggcagca
     8881 cctccaaacc atagaggcgg aagaaatcag cgatacgcgc ctcgcacttg tccactcgag
     8941 caaatatctg aaccgtttgg aatcttgcca gccccaaaaa ggcaagattt tccgcctgga
     9001 tgacgacact gcaatcagca caggctcgct gtctgccgca cgctttgccg ccggttcggc
     9061 agttcaggca gtcgacatgg tcatgaaccg caaagcatgg catgcctttt gcgccgcccg
     9121 cccgcccgga caccatgcgg gcagcggcaa agccggcgga ttctgcctgc tgaacaacgt
     9181 tgccgccggc gtcatgcatg ccattgccga ataccgcctg aaacgcattg ccgtcatcga
     9241 tttcgatgtc cactacggcg acggtacggc agaaatattc aaagacgatc cgcgcatcct
     9301 gtttttcaac ctgtttgaaa ccgacctttt ccccttcccc gaaaacaacg atatgcccga
     9361 cggcggcaat atggtgcacc tgcccttgcc gccgggaacg ggcagccgca cattccgcga
     9421 agccgtccgc aggcagtggc tgccccgact tgccgcattc aaacccgaac tggtgctgct
     9481 atcggcagga ttcgacgcac accgtctaga cgaatcgggc aggctcaacc tgcacgaggc
     9541 ggattttgcc tggctgacac acaaaatcat tcagacggca tcgggctgcc ccggcaaaat
     9601 cgtatccgta ctcgaaggcg gctacaccct tgaacctctg gcacaatctg ctgccgagca
     9661 tatccgcgtg ctggcagggc tgggcaaatc cgatgccgca accgcctatc agaaaacact
     9721 ggacccaaca aaaaaacggt ttgccaaacc gaaaaccgga caggtgcgcc aacctaccca
     9781 atccgaccgt tacg
//
LOCUS       DAWHRS010000074         9734 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWHRS010000074 DAWHRS010000000
VERSION     DAWHRS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.074"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            26..190
                     /locus_tag="WIB82_001258"
     CDS             26..190
                     /locus_tag="WIB82_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842555.1"
                     /translation="MGQVAFYEKMIGLWSSKSREASERADLAAFEFAEGELANYREML
                     KRHLQTKSVE"
     gene            193..429
                     /locus_tag="WIB82_001259"
     CDS             193..429
                     /locus_tag="WIB82_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842556.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            401..820
                     /locus_tag="WIB82_001260"
     CDS             401..820
                     /locus_tag="WIB82_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842557.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVID
                     KMERDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNASGGCIDGFGSHGLQLYNRALGYGN"
     gene            840..1022
                     /locus_tag="WIB82_001261"
     CDS             840..1022
                     /locus_tag="WIB82_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842558.1"
                     /translation="MPTPPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQN
                     QAWRDWAGNHSRKVGN"
     gene            complement(1067..1237)
                     /locus_tag="WIB82_001262"
     CDS             complement(1067..1237)
                     /locus_tag="WIB82_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ3842559.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            1250..1468
                     /locus_tag="WIB82_001263"
     CDS             1250..1468
                     /locus_tag="WIB82_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842560.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            1465..1803
                     /locus_tag="WIB82_001264"
     CDS             1465..1803
                     /locus_tag="WIB82_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842561.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            1815..2081
                     /locus_tag="WIB82_001265"
     CDS             1815..2081
                     /locus_tag="WIB82_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842562.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            2078..2275
                     /locus_tag="WIB82_001266"
     CDS             2078..2275
                     /locus_tag="WIB82_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842563.1"
                     /translation="MSDPILDALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            2278..2784
                     /locus_tag="WIB82_001267"
     CDS             2278..2784
                     /locus_tag="WIB82_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ3842564.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(2781..3371)
                     /locus_tag="WIB82_001268"
     CDS             complement(2781..3371)
                     /locus_tag="WIB82_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842565.1"
                     /translation="MNWLNRGIQEKILRVLSDNYPNCLKATKVYNSLFPSSSLPITSN
                     IPVDKQGLGLALTLGCEIQSQHLATLQSDEFQYFLKNIYYLEESGLIEITSIDSLHKN
                     FDCKINHKGIDFLTDDGGLSAILGVVTVKLHSDTIQALIAAKIDQAEISDSEKSWLKK
                     ELGKIKDTALSTLTENAINAIPAATLITLLKKFVGL"
     gene            3384..5006
                     /gene="terL"
                     /locus_tag="WIB82_001269"
     CDS             3384..5006
                     /gene="terL"
                     /locus_tag="WIB82_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ3842566.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTK
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRVTG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            5006..6574
                     /locus_tag="WIB82_001270"
     CDS             5006..6574
                     /locus_tag="WIB82_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ3842567.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEECDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     NGTTATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            6561..7856
                     /locus_tag="WIB82_001271"
     CDS             6561..7856
                     /locus_tag="WIB82_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ3842568.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            7966..8382
                     /locus_tag="WIB82_001272"
     CDS             7966..8382
                     /locus_tag="WIB82_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ3842569.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            8613..9674
                     /locus_tag="WIB82_001273"
     CDS             8613..9674
                     /locus_tag="WIB82_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842570.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
BASE COUNT         2567 a         2276 c         2699 g         2192 t
ORIGIN      
        1 gtaaacgcaa gaatcgtcgt taattatggg acaggttgcg ttttacgaaa agatgattgg
       61 gctgtggtcg agcaaaagcc gtgaggcaag cgaacgggct gatttggcgg cgtttgaatt
      121 tgcggagggc gaactggcca attatcggga aatgctgaaa cggcacctgc aaaccaaaag
      181 tgtggaatag caatgcgtat tttggatatt tttaaaaacc ccgcgacagg caatgtgtcg
      241 cactcgaaac tgtgggcaaa cgttgcctgc gcggcgggga cgtttaagtt tgtgatgctg
      301 cccgatccgt cggcggagat ttgggcggtg tatttgggca ttgtcggcgg ctatgcggtg
      361 gcgcgctcgt ttgtcagcgt caaacgtcag gaggtcgaga atgaatctcg tgaaactgct
      421 ggcgaataac tggcaaccga ttgccatcat cgcgcttgtc ggcacgggct tggcggtgtc
      481 gcaccatcaa ggctacaagt cggcatttgc gaagcagcag gcggtcatcg acaagatgga
      541 gcgcgacaag gcgcaagccc tgctgttgtc ggctcaaaac tatgcgcgcg aactggaaca
      601 ggcgcgtgcg gaagcgaaaa aatatgaagt caaggcgcac gctgtcggca tggctttggc
      661 gaaaaaacag gcggaagtca gccgtctgaa aacggaaaat aaaaaggaaa tcgaaaatgt
      721 ccttactcaa gaccgtaaaa atgcaagcgg cggttgcatt gacggctttg gctctcacgg
      781 cctgcagctc tacaaccgcg ccctcggcta cggaaattaa ggttgtcgaa aaggcagtca
      841 tgccgacccc gcctgctgca ttgatggtcg cgccggtacg cccgaatccg ccgaaagacg
      901 gcaagacggc cacgctgttg gaacacgccg ctgagtttgg tggctatgtg gcggagttgg
      961 aaaatcaaaa tcaggcttgg cgcgactggg cgggcaatca ctcccgcaaa gtcggaaact
     1021 gacaaaaaag cccgcgtagg gcgcgggctg agggtgaaag cggattttat acctctttta
     1081 caggggtagc ggcggtagtg cttttcagca aatcgactgc gtgctgacag ttttgcttgc
     1141 tggtgtagcc ttcgccctga gcgatgattt catggttggc tgctttcaaa cgccaacggt
     1201 attcgccttt tgcgtcttta tagatttcaa aatacataag gtttctccta tgaatgagta
     1261 cacgttttct taccgcttta acggcaagtc ctggtcattg agcatttggg cggacaaccc
     1321 tgaagaagcc agggcgaaat ttcgggctgc acgagaaaat gcgcactatg acggcgaagt
     1381 tgtagcaaag gtttatacat ttgtaaatat ttcgtgggtt aagaaattgt acaagcggac
     1441 aaaatattta atgggtatca aagaatgacc taccgtgaat tagttgaacg tcagttggct
     1501 gtgcgccatg ccgatttgga attgggctta agccgcgcac gcgagcaaga gccgtttgtc
     1561 attcatgttt ccgatctgtt ggataaggca ggcattgagt acgcggtacg catggataag
     1621 gattttcaga cgacgtttca ccttgaatat ccaattacga actatgacac ctttaaacgt
     1681 gcggtttggc aaactttggg ggcgtattac tgtgtttgta atgatggtga tggactggag
     1741 attgccagca atcgccctga cggttacgcc gtccgtatcg tattcggcga tgtgccggtt
     1801 taaaggggtt ttaaatggac tttgaatttg gtttcagaac cctgtggccg attgcgacgg
     1861 cggcattttg gttttgggtc aacggcattt caggccgcct gaaagaggcg gacaagcgta
     1921 tcgacgacct taaagaggag ttgcacgcgg tcaagctctc ttatcacacc aaggcggacg
     1981 ccaaggcaga cagcactaat attgcggcgg ccttggagcg aattgaaaac aagttagaaa
     2041 aagtaaacga aaaactggac aggaaagcag acaaatcatg agcgacccga ttttggatgc
     2101 cttggcgcgt attgaaaaca agactgatca aacgctgaaa aatcagaagg aaatgcaggc
     2161 ggaaattgcg caaattcgcc aagacacgaa acgcacggcc attacattcg gcgcactggg
     2221 cggcggcgtg attacggtcg gctgggaatt gcttaaagcg aaaatgggac tgtaattatg
     2281 gctcacccgc aagaaatccg tgaaaagtta cgccggctct atgtgagcgg cgagcaaact
     2341 ttggaaacgg cggccttgat gtgcgaaatc ccgcagacca ctgcgcgtgc gtggaaacgt
     2401 gcggataagg aaaaaggcga cgactgggat aagatgcgcg ccgcttacac tttggccggc
     2461 ggcggtattg aggatttgag ccgtgcgatg ttggccggtt ttatggtgca gtacaacagc
     2521 acgatgacga tgctgcagga ttcgagtacc gaagatttgc caccatccga ccgcgccaag
     2581 ctgttggcca gcctggccga tgcgtttacg aaaaccgtat cggccaatgc gcgtgtgatg
     2641 ccggaaacgt caaaactggc gacggctttg gaattgattg agttcttgat ggcgtttgta
     2701 caagaaaaac atcccaaaca tttgcctgcc tttgtggagg tattggagcc gtttggggtg
     2761 gaagtggaga agaagtttgg ttagaggcca acaaattttt ttaaaagagt aatgagggtg
     2821 gcggcaggga tagcattaat tgcattttcc gtaagcgtac ttaatgcagt gtccttgatt
     2881 ttccctaatt cttttttcaa ccagcttttt tctgaatcag aaatctctgc ttggtctatt
     2941 tttgccgcaa ttaaagcctg aatagtgtcg ctgtgtagtt tgactgtgac aacaccaaga
     3001 atggcggata ggccgccatc atcggtaagg aagtctatgc ccttatgatt aattttgcag
     3061 tcaaaatttt tatgaagtga atctatcgaa gtaatttcaa ttaaaccaga ttcttctaag
     3121 taataaatat tttttaaaaa atattggaat tcatctgatt gtaaagttgc taggtgttga
     3181 ctttgaattt cacaaccaag agtcaaagcc aagcccaatc cttgtttatc aacagggatg
     3241 ttagaagtaa taggtaaaga actactaggg aaaagagagt tatacacctt ggttgctttt
     3301 aggcagttcg ggtaattatc acttaaaact cgtaagattt tttcctgaat acctctattt
     3361 aaccagttca taaattattc ctcatgaaaa caaaagaatt cctcaaatcc cttgccgaac
     3421 tggccgccag tttgcgccaa gtcatcgaag cggaagtgga cggcttcgat gcgtcgccca
     3481 aggctattgc tgcacgccgt gccaaggtgt ttgacccggt aggcggttac gagtatttcg
     3541 tgaataccta cttcccccat tatatccgct cgcctgagaa atccgaactg catgcgtttt
     3601 tattcagccg tctgccggag attatccgct cccccaaagg ggaaaatgag gcggtgggtg
     3661 cgccgcgtgg agagggtaag tcgacgaagg ttactcagtt gtttacgctg tggtgtattg
     3721 tgaccggcca aaaacattat gctgttattg tgatggacag tatcgaccag gcatatccga
     3781 tgctggaagc catcaaggcg gaacttgaat ttaacccgcg cttgaaaacc gactttccgg
     3841 aagtatgcgg gcagggccgt gtatggcagg ccggtacgat tgtgacggcc aatgacgtta
     3901 aagtccaagt ggccggtagc ggtaaaaagc tgcgcggttt gcgtcacggc ccttaccgtc
     3961 ctgacttaac tgttttggac gatattgaga atgacgagca agtccgcaac cccgaacagc
     4021 gcgacaagct caatgcgtgg ctgactaaga ccgtattgcc tctgggcggt gtcggtcaga
     4081 aatacgatgt gatttatatc ggcacgattt tgcattacga cagcgtactt aaccgcactt
     4141 tgaataaccc gttttggcac ggtattaagt ttaaggcgat gaaacgctgg cctgaccgca
     4201 tggatttgtg ggacaggtgg gaggaacttt tccgaaacga cggcgagacg gtggccgagg
     4261 cgttttatca ggcaaacaaa gacgagatgg agcgcggcgc ggtcacttct tgggcggcgc
     4321 gtggcgtact cgcgctgatg aaaatccgtg cgcgtgacgg ccatgcgacg tttgattcag
     4381 aatatcaaaa cgatccggtc agtggcgaag atgcgccgtt tgccaagtcg atgaagtttt
     4441 ggaacgacct gccgtccgat ttggtgtatt tcggtgcgct cgacccgtca ctcggaaagg
     4501 ccggggcgag ccgtgacccg tccgcgatta tcattggcgg ttatcaacgt gtaaccggca
     4561 aactgtatgt cgtggaggct cagattaaaa aacgtctccc tgatttgatt attgaggacg
     4621 ttattcgatt gcaccgtcaa tatcgttgca aactgtggtt tgttgagact gttcaatttc
     4681 aggaatttct gaaagacgag ctggtcaagc gcagcgcggc gcgtggaata cctgtcccag
     4741 cgcgggcggt caaaccggta tcggacaagc tgttgcggat cgagacttta cagcctcaca
     4801 tggcgaacgg tttgattctg ttgaatgaga gccaacaaac gctgatacag cagttccgcc
     4861 attttccaaa ggctgatcat gatgatggtc ctgatgccgt gcatatgctc tggtcggggg
     4921 cggtggccaa ttgtgtgccg atagaatggc aaagccctac cgataacgat tttgatgacg
     4981 agataaaaag taaatggagc cgataatggc aaaaaagaac aataaaacta aaatccaaaa
     5041 gcccgaagct gcattgcaga cggacgtggc tcaaattacg gcgaccggtc gggttatcgc
     5101 cgagcatccg tccaatttta ttacgccgca aaagatgcgg gccctcttcg aggacgcaga
     5161 aagcggcgac atccgcgccc aacacgagct tttcgcggac attgaggagt gcgacagcga
     5221 catcgcggca aatatgggga cgcgcaaacg cgcgctgctg acgctcaact ggcgcgtcgc
     5281 cccgccgcga aatgcgacgc ccgaagaaga aaagctgtcc gaccaagcct acgaaatgat
     5341 ggacagcctg cctaccctcg aagacctgat tatggatttg atggacgcgg tagggcacgg
     5401 attttctgcg ttggaggtcg agtgggtatt ttcagacggc ctttacctac cccgaaactt
     5461 tatccaccgc ccgcaaagct ggttcaaatg ggacaaagac aacgggctgc tgctgcgtac
     5521 ccgcgaaaat ccggaaggcg aagcgttgtg gccgctgggc tgggtcgttc atacccaaaa
     5581 atcgcgcagc gtccagcagg cgcgcaacgg gcttttccgc acgctttcct ggctgtatat
     5641 gttcaaacac tacgccgtcc acgattttgc cgagtttttg gagctgtacg gcatgcccat
     5701 ccgtatcggc aaatacggcg cgggcgcaac taaagaggaa aaaaacaccc tgcttcgagc
     5761 ggtggcggaa atcggtcaca acgcggcagg catcatgcca gaaggtatgg aaatagagct
     5821 ccacaacgcg gcaaacggta cgacggcaac cagcaatccg tttttgcaga tggccgactg
     5881 gtgcgaaaaa tcggcggcgc ggctgatttt ggggcaaacg ctgaccagcg gtgcggacgg
     5941 aaaatccagc accaacgcgc tgggcaatat ccacaacgag gtacgccgcg atttgctggt
     6001 gtcggacgca aaacaggtgg cgcaaaccat cacaagccaa atcatcggac cgttcctgca
     6061 aatcaactat ccccatgccg acccaaaccg cgtgccgaaa tttgaatttg acacgcgcga
     6121 gccgaaagac atcgcggtct ttgccgacgc tatcccgaaa ctggtggatg tcggcgtaca
     6181 aatccccgaa agctgggtgc gcgacaaact ggtcattcca gatgtgcagg agggtgaggc
     6241 tgtgttggtg cggcaggtac cggacaatcc ggtaaacaga actgcattgg cggctttatc
     6301 cgcccacacc gtaccatcta aggctacggg caggcatcag gaaatattgg acggcgcgtt
     6361 ggatgacgcg ctggttgagc ccgatttcaa ttctcagctc aacccgatgg tgcgtcaggc
     6421 ggttgccgca cttaatgctt gcaacagcta cgaggaggca gatgccgcac tgaatgcgct
     6481 ttatccgaat ttggacaacg cgaaactgcg tacctatatg cagcaggcct tgtttatcag
     6541 cgatattttg ggacaagacc atgcccgcgc ctgatttggg atttgcctta agtctgccgc
     6601 caaaaaaggc aatcgagtgg ctggaaagta aaaaggttac ggcggagagc taccgcaatc
     6661 tgacagcctc cgaaattgcc aaagtctata cgattgcccg catgaccgac ttggatatgc
     6721 tcaacgacat caaaacttcg atggttgaat cggcaaaaag tggacagtcg tttgacgatt
     6781 ggcgaaaagg tatcttgaat ctgctcagca acaagggctg gctgcatccg aacgggcata
     6841 acggtaagga tatcatcgac ccagccaccg gcgaggtatt cggttcgccg cggaggttgg
     6901 agacgattta ccgtaccaat atgcaaactg cctacaacgc cggtcaatat caaggatata
     6961 tggcaaatat tgatgcacga ccttattgga tgtatgacgc ggtaggcgac agccgcaccc
     7021 gtccggcgca ttcggcaata gacgggctgg tgtaccgcta cgacgacccg ttttgggcaa
     7081 cgttttaccc gcccaacggc tacaactgcc gctgctcggt catcgcgctg tcggagcggg
     7141 atgtggaacg ccaggggcgg attgttgggc aaagcacggc ggacaatctg gtcgagaccc
     7201 ataaaatcta caacaaaaaa ggcgatactt atctgaccct tgcctataaa gcaccggatg
     7261 gcagtctgta cacgaccgat cgaggatttg attacaacgc cggacgaatg aactaccgcc
     7321 ccgatttaga caagtacgac cgtgcgttgg cgcatcaatt tgccaaagcg gaaatgggtg
     7381 gtgcggattt taaaaccagc tttaaacagc ttgaaaaaga gttttatgaa gtcaagcaac
     7441 gtttggatat tgatggcaag cccgataaag agcagaaaat caaaatccga aatgcgctat
     7501 caagacagct taaatttgct gcgggtgtat tgagcaagga aacgcaagaa ttggcaggta
     7561 tgacacgagc gacggtgtgg ctgtctgatg atacgttggt taaacaggta gacagccgtg
     7621 aggggcagaa tttcgatgac tcctactatg cttttttgcc ggatatgctg caaaaccctg
     7681 aacatgtcat ccgcgacaat cgtgaattga ttttcacagc tcgctataaa ggctcggcat
     7741 tgtgggcagt tttaaaatat attaaggagg tggatgagat ttatctacag tcgtaccgaa
     7801 tcagtaacga caaagagatt gccaaattta tggcgaagaa gaaagtattg aaatagacgt
     7861 tgggcaaggc tcgaaatcac ttgcacacgc tctcggacgc cctaacgggc aggctgcggt
     7921 atcgaggtta tcaccgcttt tccaacgtct gtacggaata ataccatgat tgatgtcaaa
     7981 atagacaata tctttgtcgt cctaaaccaa atcgagcggc ttggcaacgg gatcgaaaac
     8041 cgctacctgc tgatgcgccg actgtccgaa accatgcaca cggcggtcaa gctcaatttc
     8101 cgctacgcag gccgtccgaa atggttgggg ctaaaatacc gcgacggcaa gccgctttcg
     8161 gattcgggtc gtctgaaaga cagtttttcc acactgtcag acaacgatac agcccttgtc
     8221 ggtacgaata tcgtctatgc cgccatccac aacttcggcg gtatggcggg gcgcaaccgc
     8281 aaagttcgga ttccgcaacg ggaatttttg acgctgacgg acgacgacaa acaggctttg
     8341 atggacgatg tgcaggatta tttttcgggt ctgataccgt gaatttataa aaccctcaaa
     8401 aacgcgcttt ttagcgcgtt tttttatgcg ggtaatacaa acccctgccc aagatataaa
     8461 aatcaatcct agacgcttct aaaaagcccc tgaaaacgat taattgtgta tcgcgcggac
     8521 aggttttaaa aaaatggcgg gagggtttga agcacgccta ctctttgttg ttttttcaaa
     8581 taggcaaaat gaccgtattg agagaggtac acatgtccaa aaatgcacaa aaaaccctac
     8641 ttgccgtgtg cagtttcgag gtgcagccaa aagacgggcg aatccaactg ctgccatatg
     8701 gcgaatttcg cgcagtagac ggtcgtccga ctgatgtccc tgcgtggtat ctgaccgaag
     8761 aaaacggtca tgatgtcgcg ttgttggcca acagctcgcg caatcagttg gttgtcgatt
     8821 atgaacacca gacgctctac aaagagaaaa acggacaacc tgcacctgcc gccggttgga
     8881 tgcgttggct ggagttcacg cctaaaggca tgtttgccga agtggagtgg acggacaagg
     8941 cggctgcggc aattgccgca aaagagtatc gctacatctc tgctgtgttt tcctatgaca
     9001 caaagggata tgtaagcaaa atttttcacg ccgcgctgac aaatttcccc gcgttggacg
     9061 gtatggacga ggtgctggcg gcagcgtcgg cgcaaatttt aaaaccggaa acggagcaaa
     9121 accctatgaa agagttgtta cagcaactgt tcgacctgcc tgatgcgggc gaagaagaac
     9181 tgaaggcggc attgtccgcg ctcgtggaag ccaagccgaa agacgtggca ttgtctgccg
     9241 acgtgttcgc gcagctggcg gaaaaagaca gccgcatcgc ggcattgacg gcgcaaaccg
     9301 ccaagcctga tttgactaaa tacgcgccta tctcagtggt tcaagagctg caaagcaaag
     9361 tcgccgcgct gactgccaag caggaagcag acaaaggcaa cgaattgatt accgccgcgc
     9421 tgacttcagg caaattgctg cctgctcaga aggagtgggc aaaaggcgta ttgaaacagc
     9481 cgggcggctt ggcatttttg accggcttta ttgaaaacgc ccagccggtc gctgcactgg
     9541 caggctcgca aacgggcggc aaagcacccg acgaacgcgt cgccgcactg actgcggaag
     9601 aggcagccgc agcaaaaatg ctgggcatgt ccggcgaaga atttgtaaaa atcaaagaaa
     9661 gcgaaggtaa gtaatggaca aatcagcgat tttgaccgca atcacggcag cattccgcaa
     9721 agaatttcaa acgg
//
LOCUS       DAWHRS010000075         9730 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWHRS010000075 DAWHRS010000000
VERSION     DAWHRS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.075"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(69..836)
                     /locus_tag="WIB82_001274"
     CDS             complement(69..836)
                     /locus_tag="WIB82_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ3842571.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACHLNQDLGKSLLDLGFKKIPSVYPE
                     YEAYEDKRSIPENPYIHELQYIKKGENPAIITHQNYHRYGENDYSTDIGSCINGFTVQ
                     YYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(1300..1575)
                     /locus_tag="WIB82_001275"
     CDS             complement(1300..1575)
                     /locus_tag="WIB82_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ3842572.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(1572..2855)
                     /locus_tag="WIB82_001276"
     CDS             complement(1572..2855)
                     /locus_tag="WIB82_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3842573.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(3011..4429)
                     /gene="glnA"
                     /locus_tag="WIB82_001277"
     CDS             complement(3011..4429)
                     /gene="glnA"
                     /locus_tag="WIB82_001277"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ3842574.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDTTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(4703..5080)
                     /locus_tag="WIB82_001278"
     CDS             complement(4703..5080)
                     /locus_tag="WIB82_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842575.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            5251..6060
                     /gene="aroE"
                     /locus_tag="WIB82_001279"
     CDS             5251..6060
                     /gene="aroE"
                     /locus_tag="WIB82_001279"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ3842576.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGLGLVGDIVKVQHTEIEGKNILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELSRLFGIEAVPMADLNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAL"
     gene            6063..6764
                     /gene="mtgA"
                     /locus_tag="WIB82_001280"
     CDS             6063..6764
                     /gene="mtgA"
                     /locus_tag="WIB82_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ3842577.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LKRMGSAELPESDTD"
     gene            complement(6967..7042)
                     /locus_tag="WIB82_001281"
     tRNA            complement(6967..7042)
                     /locus_tag="WIB82_001281"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7007..7009),aa:Phe,seq:gaa)
     gene            complement(7233..7967)
                     /gene="lptB"
                     /locus_tag="WIB82_001282"
     CDS             complement(7233..7967)
                     /gene="lptB"
                     /locus_tag="WIB82_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ3842578.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(8041..8571)
                     /gene="lptA"
                     /locus_tag="WIB82_001283"
     CDS             complement(8041..8571)
                     /gene="lptA"
                     /locus_tag="WIB82_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ3842579.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVIIQPSSTQKSE"
     gene            complement(8552..9133)
                     /gene="lptC"
                     /locus_tag="WIB82_001284"
     CDS             complement(8552..9133)
                     /gene="lptC"
                     /locus_tag="WIB82_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ3842580.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(9130..9666)
                     /locus_tag="WIB82_001285"
     CDS             complement(9130..9666)
                     /locus_tag="WIB82_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3842581.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         2338 a         2551 c         2394 g         2447 t
ORIGIN      
        1 cacgcaaggt ttggatttgt tctggtgtta aaacaacttc accctcattc ataataagat
       61 cctttaaatt attttttact tgaattgttt acaaaactct gtaccaattg ctctacttgt
      121 tggtgataac ctaccaactc ctgctgtgtg agctgctgct tttcccgaat aaacgggtaa
      181 tactgtaccg tgaaaccgtt aatacaggaa cctatatcag tactgtaatc gttctctcca
      241 tacctatgat agttctgatg agtaataatc gccggatttt ctcctttctt aatatactgt
      301 agttcatgaa tatatgggtt ttcaggaatc gaccttttat cttcataagc ttcatattca
      361 ggatagacag acgggatttt tttaaatccc aaatcaagca acgattttcc taagtcttga
      421 ttcaaatgac acgcatcttt tcggataaca atatcgtcca cgctatccaa accatcgcgt
      481 agaaattgaa taaagtgtgc tttgttctgc tggttgtatg gttcgagcac ccagccttgg
      541 tcggtttttt tgtaatagac gctaatctct atcaaattaa tggagtaagc atcctgcccg
      601 ggcgtgtcgg aaagccattt tgtggcattg tcttgcgtat accgtgcaag atagccataa
      661 gggataacca ttttggtgcc ctgaaaatca aatgtaaccg tatgttcaaa tcctgtcatt
      721 ggctcgggat tgttgaaact ggtttgttca ttaacaggtt cacatgaggt catagtaaaa
      781 acactcccaa taaacaaaat aaaaagttga taaatggtag tagcatatgg tctcataatt
      841 tcaagcctag aaattagtta aagaatatat tatggtagat actaactctt taagttgaca
      901 tattgtgcga tatcgagggc tgtaccgtat tttcccatcg tattttccca tcgtattttc
      961 ccatcgtatt ttcccatcgt attttcccat cgtattttcc catcgtattt tcccatcgta
     1021 ttttcccgtt ctttatatga gcggcaggca tcctcataaa agaagggata ccgataagga
     1081 aaaatacccc agcataaaaa atatactgat taatcaatac aaatccaaca aacagttcat
     1141 cgtattcttt tctgttggcg tataaaaaat actgatatat gccttcactt tcatagggcg
     1201 acgctttatg taccgtcctg tgtgttgaaa cgtcggtttt ccttaccgcc tgccccgctt
     1261 ttccctgatt gctgccaact gccgtccgaa cctgaaccgt caggtttcag acggcatttt
     1321 ttctgcctgt tcccacaatt tccgtttttg tgcggcggtc agttttttga cggcgatttc
     1381 ctgcctgagt gccgtgcctt tatccatccc gcctgcaacg atacgcatcg ccaccggttt
     1441 gaatacgcgg gtatatttcg cgcctttacc ggttgtgtgg gcggcaagcc gctgttgcgg
     1501 attcgggctg atgccgcaat agaacgcgct gttttcacac aatatcaggt aaacgctcca
     1561 gtttgacgcg ttcatctgcc tgcatcctga gttttctccc cgttccaagg cgcaactttc
     1621 agcagcagca gcatacccgg tagggcgagt gcgaaacaca gttggaaaaa cggtacataa
     1681 ccgagccatt caatcagata accggcaaag gaattgatga ccgtgcgcgg gacggcggac
     1741 aggctggtaa acagcgcaag ctgcgttgcg gtaaatgcgg gattggtttc acgcgccata
     1801 tacgatacga acgccgccgt ccccaacccc acgccgaccg cttccgcgcc gataactgcc
     1861 gccagcatca gcctctcgcc tgtgccgacc gtgtcgaaag gtccgaaccc tgccagccat
     1921 acaaacccca aaacggttac agcctgcacc gcgccgaata gccacaaggc tttgtttacg
     1981 ccgattttca gcatccacac accgcccaag atacctgccg ccactgccgg ccacagtcct
     2041 gcatttttcg caatcaaacc gatgtctgtc ttgctgaaac ccatatccag ataaaacggc
     2101 gttgccaacg cggttgccat actgtcgccg agtttgtaaa ggaagataaa cagcagcacg
     2161 cacaccgccg aagcgatgcc cttgcgcgta aaaaattctt taaacggctc taccacggtc
     2221 tgcttcaacg ttttaggaac ggcaggaggc aacacgggtt cgcgcgcaag aaacagcgtc
     2281 atcagaaggc cgggcagcat aaataatgaa gtgataacaa atacttctga ccacggcatc
     2341 ctgtctgcca acaccaaact caatgaaccg ggaatcaggg cggcaatccg gtaggcgttc
     2401 acatgaaccg agttgcccaa acccaattct tcgtctgaca aaatctcgcg cctgaacgca
     2461 tccaatacaa tatcctgact ggcggaaaaa aaagcgacaa gcaccgacaa gccggcaatc
     2521 agcggcagat gattacgggg gtttaaaaag gcataggccg ccaaagccgc cagcaaccct
     2581 gcctgcgtca gcagcatcca cccgcgccgc cgtcccaaaa cgggcagcct gaccgcgtcc
     2641 atcagcggcg accacaaaaa tttccaagta aacggcagac cgattaacgc catcagcccg
     2701 atgctcttca aatccacctg ctcgctgcgc aaccacgccg gaatcaggtt aatcagaaag
     2761 tacagcggca gccccgaggt aaaccccgta aaaatacaga caagcatatt tcgggaaaag
     2821 atttgcccga taaaacctga tttcgatgca gtcatcgttc gtgcagccca aaaaaaacaa
     2881 aagcctattg tagcaaatat cgcccgcctg ctgaaaaatg ccgccccgcg ccgccagttt
     2941 gccgttcaaa tgcaaaatgc cgtctgaaac tgtgttcaga cggcattgct tttctgaaaa
     3001 ccagacgtgc ttacaggctg taatacattt caaattccag cgggtgcggc gccatgcgga
     3061 tgcggcgtac gtcttcttct ttgaaagcga tgtagctgtc gatccagtct ttgctgaaca
     3121 cgccgccgcg caggaggaat tcgtggtcgg ctttgagggc ggccagtgct tcttccaaag
     3181 aagcgcaaac ggtcggcacc aatgcatctt cttccggcgg cagatcgtac aggtttttat
     3241 cggcagggtc gcccggatgg attttgtttt gaatgccgtc caaaccggcc atcaacaggg
     3301 cggcaaatgc caaatacggg ttggcggtcg gatcggggaa acgcgcttcg atgcggcgcg
     3361 ccttgctgct gttcacggac ggaatgcgga tggaagcgga acggtttttg gcggagtatg
     3421 ccagtttggt cggcgcttca aagtgcggca cgaggcgttt gtaggagttg gtggacggat
     3481 tggtaatcgc gttcaaggct ttggcgtgtt tgatgatgcc gccgatgtag tagagcgcgg
     3541 tgtcgctcaa gccggcatag ccgtcgcctg cgaacaggtt ttgaccgtct ttccaaatgg
     3601 attggtgaac gtgcataccg ctgccgttgt cgcccataat cggtttgggc ataaaggtgg
     3661 ctgttttgcc gaagttgtgg gcaacgtttt gaatcacata tttcatgtct tgggtttggt
     3721 cggcgcgttt gaccaaggtg gcgaaacgcg tgccgatttc catttggctg ccggtaccga
     3781 cttcactgtg gtggacttcg acttcgatgc cgagttcttc caaaatgttt accatcgcgg
     3841 agcgcaggtc ttgtcccgcg tcaatcggag caacgggcgc gtaaccgcct ttgacggcag
     3901 ggcggtggcc ggtgttttga ccgtccatat gcagaccgct ggcccatgcg ccgctttcgg
     3961 acgtgatttc gtaacgggtt ttgtgcatat cggtttcaaa ttctacgccg tcgaagacga
     4021 agaattcggg ttcggggccg aagtatgccg tgtcgccgat gccggaagat ttcaaatagg
     4081 cctcggcgcg gcgtgcgatg gagcgcgggt cgcggtcgta accctgaccg tcggcagggt
     4141 cgatgacgtc gcaggtaatg acgacggtgg tatcgtcgta gaaaggatcg acgaaggctg
     4201 tagacgcatc gggacgcaac tgcatatctg aagcctgaat acctttccaa ccgccgatgg
     4261 acgaaccgtc aaacgcctgt ccgttttcaa accactcttc ggggtcttcc aacacgatgc
     4321 gcgcaggcac ggtaaagtgg tgctgcttgc ctttggtatc ggtaaagcgc aaatcgacaa
     4381 agcgggcttc gctttcttca atcaatttta cggcgttttt aatagacatt tttcagctcc
     4441 tggaaaaggg aaatagcaaa atatgcggaa cgcggcagat tagctcaaag ccagcatgat
     4501 tctaccacgt ccatgccgtc tgaaactggc cctgcgtcaa attccaagct gtaaaatata
     4561 gtttcagacg gcaatattgt caacaatatg cttcggtttg cccgttccca agcagaatgc
     4621 ccgccctttt atccgaatgc cgacaaaaca gatgccgtct gaaagcagtt cagacggcat
     4681 ctgttgtttg gtttgaaaac ggtcagcgag gatagccgtt gcagacaaat ttgtactgag
     4741 cctctccaag cgtaccggga ataacataat tccattcggc ataggatggt gtataagaag
     4801 cgatagagtt tcccgatttg gaataccacg ttgccgtcaa tatcctaaaa cgtttaccac
     4861 tgcaatccat ttcttccaac gttttagctg tattaaaaaa ttttccattg ccatactttt
     4921 gcggcttggc gaaccttgtt tcaaaccatg cactgttgtc cgtacggctg tctgcatcac
     4981 caaacgtggc agtattacta tctaccaata cccaatccgc cgccaccgcc ggcgttgcca
     5041 accccaatgc aaatactgcc aagcacaaca tctttttcat gtccgaccct ctcgtccttg
     5101 tcaacaaaat aaagttaaat tgtaaaaaaa aacgactttt ttcaagagaa aaatattccg
     5161 tcatgctatc tgacaaccgt ttcagacggc atcgtttcca tctgtacgcc gccgttataa
     5221 aatagccttc ccgtcttttg gaacacgacc atgaccgccc tcccccgtta ctccgttttc
     5281 ggcaaccccg ttgcacacag taaatcgccg caaatccatc agcaatttgc ccttcaggaa
     5341 ggcgttgaca ttgaatacga acgcatttgc gccgacatcg gcggttttgc acaggcggtt
     5401 tcgacatttt ttgaaacagg cggttgcggg gcaaacgtta ccgtaccgtt caaacaggaa
     5461 gcgtttcatc tggcggacga gcattccgaa cgcgcattgg cggcaggtgc ggtcaatacg
     5521 ctgattccgt tgaaaaacgg caagctgcgt ggcgacaaca ccgacggttt gggtttggtc
     5581 ggcgatatag tgaaggtgca acacacagaa attgaaggca aaaatatcct attgctcggc
     5641 gcgggcggcg cggtgcgcgg cgtgattccg gttttgaaag aacaccgtcc tgcccgtatc
     5701 gtcattgcca accgtacccg cgccaaagcc gaggaattgt cgcggctttt cggcattgaa
     5761 gccgtcccga tggcggattt gaacggcggt tttgatatca tcatcaacgg cacgtcgggc
     5821 ggtctaaacg gtcagattcc cgatattccg cccgatattt ttcaaaactg cgcgcttgcc
     5881 tacgatatgg tgtacggctg cgcggcaaaa ccgtttttag attttgcacg acaatcgggt
     5941 gcgaaaaaaa ctgccgacgg actgggtatg ctagtcggtc aagcggcggc ttcctacgcc
     6001 ctctggcgcg gatttacgcc cgatatccgc cctgttatcg aatacatgaa agccctataa
     6061 acatgttccg catcatcaaa tggctgattg ccctgcccgt cggcatcttt atctttttca
     6121 atgcctatgt gtacggcaac atcattacct accgcgccgt cgcgccccat cggactgcct
     6181 ttatgtcgat gcggatgaag cagtttgaac aggaaggtcg cgatgtcgca ctggattacc
     6241 gctggatgcc ctacaaacgc atttccacca acctgaaaaa agccctgatt gcttccgaag
     6301 atgcccgttt cgccgggcac ggcggcttcg attggggcgg cattcaaaac gccatcaggc
     6361 gcaaccggaa cagcggcaaa gtgaaggcgg gcggctcgac catcagccag cagcttgcca
     6421 aaaacctgtt tttaaacgaa agccgcagct atatccgcaa aggcgaagaa gcggcgatta
     6481 ccgcgatgat ggaagccgtt accgacaaag acaggatttt tgaactgtat ttaaactcaa
     6541 tcgaatggca ctacggcgtt ttcggcgcgg aagccgcgtc ccggtatttt tatcaaatac
     6601 ccgccgccaa gctgaccaaa cagcaggcgg caaaactgac ggcgcgcgtc cccgccccgc
     6661 tctactacgc cgaccatccg aaaagcaaac ggctccgcaa caaaaccaat atcgtgctca
     6721 aacgcatggg ttcggcagag ttgcctgaaa gcgacacgga ctgagtgccg ccgcaatata
     6781 aaatgccgtc tgaaatatca ttcagacggc atatgctttc tgcctttacc ctcttctcca
     6841 aaacgcaacg gggcggtcat tatcattttt gcccttctac ataacggtca gacacaaaac
     6901 gtgtccgaca cggacaactt cccaaaaacg gcaggcaaaa aaaataaccg cttcaaggcg
     6961 gttttttggt ggccagaggc ggaatcgaac cgccgacaca cggattttca atccgttgct
     7021 ctaccaactg agctatctgg cctttgtctg tctcgttaag agatgcgtat taaaccaaat
     7081 tacgggacga catgcaagca tatcgggcaa tattctttga aaacaggttt aagtcaatgt
     7141 tttaaatttg aattaattta tgtaaaaaca acataaatcc gtatttttgc cgcctgcccg
     7201 acgatattag gtcgcccgtc tgaaaaatat tttcaatatt tgaagttctt acccagataa
     7261 acagaacgaa cctgttcgtt tccgaccaaa tcatcaggtt ttcccgatgc caacaccgtg
     7321 ccgtctgaaa taatgtaggc ccgatcgcag atgctgaggg tttcgcgtac gttgtggtcg
     7381 gtaatcagta cgccgatacc gcgcgatttg aggaaaccga tgattttctg gatgtcgatg
     7441 acggcaatcg gatcgacgcc ggcaaaaggt tcgtccaaca aaataaaatg cggtttcatg
     7501 gcgagtacgc gggcgatttc gacgcgccgc cgttcgccgc cggacagcga cggcgcgggg
     7561 ctgcggcgta agtgtccgat attgaggtcg gcgagcagtt tttcgatttc cctgtcgatt
     7621 tgatttttat ctttggttct gatttccaag atggcgcgga tgttttgttc gacggtcatt
     7681 ttgcggaata tcgaggcttc ctgcggcagg tagccgacac cgaggcgggc gcgttcgtgt
     7741 atgggcaggt ggcgcaattc ttgtccgtct agggttacgc tgcctgcgtc ggcggcgatg
     7801 agtccgacaa tcatgtagaa gctggtggtt ttacccgcac cgttgggccc gagcagtccg
     7861 atgacttcgc cgctttcgat ttcgagggag aagcttttaa cgacttggcg ttttttgaaa
     7921 cttttttgca ggttttgaac aacaaggcgg ctgacgtttg cactcataaa tatctcttca
     7981 atatcttcgg tcggcatatg ccgtccgaac cgggtttata tttcagacgg cattttggga
     8041 ttattcggat ttttgcgtac tcgaaggctg gatgatgacg ctgaccctgc cggatttgga
     8101 agcggatttc gcaccggatt ttgtactgcc gctgatggta tagacttcag ttttggtgtt
     8161 gtaggtaatc accgcacctt cggcgacatc gccgccgcgc tgtactttgg cattgccggt
     8221 cagaacaacg gtgcttcctg cggaggaata ggtaacgttg tttgcctgtc cgcgcaccgt
     8281 gcctttgccg ccgtccaatg tctggctgaa gcggactggc gaaccttccg ccctcacgga
     8341 ttcgccgcct ttgccgccgc gtgtaacatt gacgcgggcg gcggaaatat tgagcgtacc
     8401 ctgtctgatg acgacgtttc cgctgaatgt ggtgctttgg ttggcttgat cgagcgaacc
     8461 ttggtcggcc tcaatctgaa taggctgcct gctgtcgctt tgaagggcaa aagcggggga
     8521 cgccgaaaaa aatgctatta aaacaaatag cttacatatc ttttgtatca taaatcgtgg
     8581 ctttcacttt agatgagaag ttcaacatgc ctgttttgtg gtcgtaagtc atgccgcccg
     8641 cctgaccgtg cgatgcacca tattggaaac tgacaggcgt atcggtttgg gcatattgag
     8701 attcggtatc gacgtgcagc ttttcggctt caactttacc cgcctgccgt ttgccgtcgg
     8761 cggttttggt cagcacaacg ttgtttttaa aaagaacctg tttgttttcg gtatggtaaa
     8821 cggcttcatc gctgccgact tcgtacaaca acctgccttc ttggaagaac acgagatgcg
     8881 gtgagtcgaa atgaatgtcg ctgctttcgg gaaactgttt cgcgcccttc gaactcaaat
     8941 gttctttcaa gtatccctgt tcgtcaaacc gcctgccatc caatccgtcc attgtgtatt
     9001 gcggttcgtc gggattgagc ctgacttctt caatctcgac ttcgctgatg cgtcccaacc
     9061 atgccgacag gctgcccaag gcaaccgcca atatcaatgg gaacgcaatt ccgtaccgcc
     9121 atcttacttt catttgatgt actcgttcaa agccgcgccc aaagtccctt gcgcctgcat
     9181 aatcaggtcg cacacttcgc gcaccgcgcc cgcaccgccg gggcgtttgg caatataggc
     9241 ggcgtgttgc agcgtaaacc aatgcgcgtc ggggacggca accggcaatc cgcagcgtac
     9301 cataacgggc aaatcgacca cgtcgtcgcc gacaaaggca cactcggctt cttccacgcc
     9361 gacctgagcg cgcagctctt catacgcggc gcgtttgtcc gaaatgcctt tgaagtagta
     9421 atttatgccc aactgtttga cgcggatgcc gacggagggc gcgtcccgac ctgtgataat
     9481 cgcagtctgc acgccgcttg cctgaagcat tttcagaccg tgtccgtcca gtgtgtgaaa
     9541 cgatttgatt tcttcgccgt tatcgcggat aaagatgcgc ccgtcggtca aaacgccgtc
     9601 cacatccagg atcaacagtt tgattttggc ggcgcgcgcc tgtaattcgg gagaaattgc
     9661 ctgcatggga tttccttgtt ttcagacggc atatcttacc acaaatgccg tctgaaacgg
     9721 ctgcaacgcc
//
LOCUS       DAWHRS010000076         9633 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWHRS010000076 DAWHRS010000000
VERSION     DAWHRS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.076"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(156..701)
                     /locus_tag="WIB82_001286"
     CDS             complement(156..701)
                     /locus_tag="WIB82_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ3842582.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAANKTATGIEALSTPKNKRWCQ
                     VLGKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
     gene            complement(844..1365)
                     /locus_tag="WIB82_001287"
     CDS             complement(844..1365)
                     /locus_tag="WIB82_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842583.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            complement(1367..2526)
                     /locus_tag="WIB82_001288"
                     /pseudo
     CDS             complement(1367..2526)
                     /locus_tag="WIB82_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            2572..2829
                     /locus_tag="WIB82_001289"
     CDS             2572..2829
                     /locus_tag="WIB82_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ3842584.1"
                     /translation="MKEFPDLEKHLIMLEVAVQDLEDRSLADSFVLSWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLELVESAQELA"
     gene            complement(2896..4869)
                     /locus_tag="WIB82_001290"
     CDS             complement(2896..4869)
                     /locus_tag="WIB82_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3842585.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRTAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDDYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSVELAEYHKDEVRVSYDLSDASVVNVFDMD
                     GKFITKAQANGNTREAFPTARIDQLAENRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNDIEAEYAVLPKTGTDDFVLFEADR"
     gene            complement(4871..5122)
                     /locus_tag="WIB82_001291"
     CDS             complement(4871..5122)
                     /locus_tag="WIB82_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3842586.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            5355..6068
                     /locus_tag="WIB82_001292"
     CDS             5355..6068
                     /locus_tag="WIB82_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ3842587.1"
                     /translation="MRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNAVSDD
                     LNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGKEHIT
                     LEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDKAETY
                     LRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVESLTEL
                     SDEDLQKTYAAVSTKKRKK"
     gene            6231..6443
                     /locus_tag="WIB82_001293"
     CDS             6231..6443
                     /locus_tag="WIB82_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3842588.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            6665..7243
                     /locus_tag="WIB82_001294"
     CDS             6665..7243
                     /locus_tag="WIB82_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3842589.1"
                     /translation="MFRHNGEILLSGINIKLNSGDSLLIRGPSGCGKTSLLRVLAGLW
                     PFGSNGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELIEAMNTCRLGYL
                     VDKLDKTDDWQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPAEAALYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            7348..8016
                     /gene="hda"
                     /locus_tag="WIB82_001295"
     CDS             7348..8016
                     /gene="hda"
                     /locus_tag="WIB82_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ3842590.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            8013..8681
                     /locus_tag="WIB82_001296"
     CDS             8013..8681
                     /locus_tag="WIB82_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3842591.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         2494 a         2663 c         1971 g         2505 t
ORIGIN      
        1 cacgcctgat ttacccaagc gcaggctcgg atgttgtttc aaagagccta cccgaacagg
       61 gcttggcgtg attttgattt caggtaacgg tggtacgcca aaatcgtttt tcaactttgc
      121 acaatgggca acacataaag caatcaaaga ctttttcata ccttcgccgc tcccaattcc
      181 atcgcaatcg cgtccgcaat cgcgcggcag atgccccatt tggtcgtctt aaacaaggcc
      241 aaatcagtgt cgttactgat aaaaaacggc tcaaacacaa tgccgcctgc ctgcgcataa
      301 gccaggcgcg aatgttgccc tgcgttatcc ggcttaaagc cgtcttcgcc gcgcagtttc
      361 cagccggttt tcttggcaac ggctttgccc agcacctgac accagcgttt atttttcggc
      421 gtggacaagg cttcgatgcc tgtcgccgtt ttgttcgccg ccgcattggt gtggaactca
      481 atcgccacat ccgagccgcg aatcagcttg accgcatcgc gcaagggcat attgcctttg
      541 ccttcgccgt cggtgcgtac ggtcaggccg taatcgttac gcaggattga agccacaatg
      601 ttgcgcatat cctgcgccaa gtccgcctca cggtcgctgc cgttgactgc gcccgggtcg
      661 gtgttactgt gtccggcagt cagacaaata attttgctca ttaaagcctc cctcaaaatc
      721 agattaaaat gcactttcag aggcttacat tttcaaacgg catggctttt gcagcgggcg
      781 aaacagtgtc agtaggcaac aaaaaagccg cctgttgttc agacggcctt agaatcgttt
      841 attttatttt ttcggcaaat tggcttcagc ttccgttatt gttttacggc attcttgcat
      901 tgattttcgg taattttgat aactttcctt atctaaatca gttgtaccgc ctccaccgct
      961 tgttaccata ctcttcatca ggatttctgc atcttgcgca gcctgtttgc agacgcggta
     1021 tgggtcatca tctccattcg tgtccgcata ataagcgact gcttgagtaa tattttcacg
     1081 atactttgca agattttctt gattaggtat ttctcctttt aaaaacctct caaaatcccc
     1141 gccaaggcct tgttgcaata cacggaaacg ggcggcgcgt tcaaaataaa aagcctgttt
     1201 ctcatctgca aattgatatt tattggcaaa aaaatactgc tccgcatcag ctttctgcga
     1261 ttgttcttgc ccacacgctg ccaaacccaa cgccagcagc acaaaataaa actttttcat
     1321 atttttctcc aaaataaaat gccatacatc tgcatggcat tccattctaa aacaaattct
     1381 cctgctccgc ttccttatcc gcctgcttta aaattcgcca cacatggcgg tcgcttaggc
     1441 ggtgtgccaa ggccaaatcg ttaacggcct cataagcagg ggtgccgcct gccgtctgcc
     1501 ggtcaaactg actgcggatt ttacggtttc gcaactcata caaggccgtc tcgcaacggg
     1561 gtataaacag attgcacgga gcagccgacc ggccgcctca ctgccgataa tctcttccaa
     1621 gtatgcaaca cgggattgac tgtttttcgt ataaccctgc cgcaaaggat aagtcgtacc
     1681 gcccatcagg cgcaccagct ccagcgtttc attaaacccg atgaccgtaa tcaacgcttg
     1741 tacactatca gggagcagat gcttgaccgc atcaggcaaa gccgctttcg caatctcaaa
     1801 gagttcgtca tccggcaaag attcgcccaa attcagcgca acagacacgc ggctgtaaag
     1861 ctgtaccagc tcgccatgct taccgcgcaa attcgcaacc agtcggggca taccgctcaa
     1921 caccaagccg cagcccgtct catcgtgcag acggcgtaca atttcaaggg cgcgcaaggg
     1981 caggttttcc gcttcatcga ccacaatcag acggcccgaa tcgcgcaggc ggtcagatac
     2041 agactcaaac aaatcattca ggctgcccat cgccgatacc ttcgccgccg tcgccaactt
     2101 gcgcatcaag accaaagccg taaagctcgg attagcctca attaagatgg ctgcagggtt
     2161 tttctcgcag tagtttttga ccgcctgagt cttgcccaaa cccgcctgac cgtagatcac
     2221 cactatttcg ccgccttcgt gcgcatcgcg catcgcttcc gcaatacggc gtgtcgtctt
     2281 agtcgataca aatcccaaca ccagctcttc acgtcgcgcc ttactttcct gcatctccaa
     2341 aaacgcctcg attttcgtct cgatggtttc ataattgccg cctttatcgg cataagtgcc
     2401 gttcagatac atactgatgg atgccggcga agtatcgata ccgcgtgcca gttgggtttg
     2461 gttcatgcct gatttttctt taaattcaac cagtttttgt tgcaatgctt gattaatttg
     2521 tttcattttt aatatccttg agttttaaac aacctttaaa ggccgtctga aatgaaagag
     2581 tttcccgatt tagaaaaaca cctgatcatg ctggaagtcg ccgttcaaga cttggaagac
     2641 aggtcacttg ccgattcttt cgtactttcc tggctgctcc agcggattac ccgtcaagag
     2701 ccgacttcga ttgagcaggt tcgcagtttc cttcaagcgc aggcaaaaac gtttgagcct
     2761 gattccgttc agcgggaaca ccttgaatct ttgcttgagc tcgttgaatc cgcccaagag
     2821 ctcgcttgag tttcaatttt tcaacaagct cggccgaaaa cgctgcattt tgctgtttgt
     2881 ccatcatgtt ttcctttatc tatccgcctc aaacaacaca aaatcgtctg tgcccgtttt
     2941 cggcaatacc gcatactccg cctcgatgtc gtttccgccc aaatgtccca gctcgtccca
     3001 aactgccgcc tgttccaaag ccggattgac ttccgcattt gcgagcttga ttgcattttc
     3061 cgcccgcttg attttgcctt ttcggcggtt ttccgccagt tggtcgatac gagccgtcgg
     3121 gaaagcctcg cgggtattgc cgttggcctg cgccttagtg ataaacttgc cgtccatatc
     3181 aaacacattg accaccgacg catcgctcaa atcgtagctg acccgtacct cgtctttgtg
     3241 atactccgcc agctcgactg aaaaataaga gttgttgaac aaatccagcc aaccgcgctg
     3301 tacctttcgc acctcttgcg gcataaacat cgtcgccagc tcttgcgccg acaacatatc
     3361 cggcgcgata ccgtcctgtt ccagcctcat ttcccgataa gccttaggcg tataatgccc
     3421 gccgtccgga tgtcggggca gctcgccgtg cgggcggttg ttgtaatcat cgatacactt
     3481 gaccacatcc gcgataaaac gcgaccagct cggcagtttt ttcaaatatt tctgttgttc
     3541 ctccgtcaaa tccttgcctt tttccaaagc attaaaagca ctttccatct tgcggtacat
     3601 caggttcttg gtactgctgt ccatccccgc gcccgcaaac gtctcatact ggcgcgccat
     3661 ctcaatcaga ttgtctttcc accatcgctc gatgatgcct cgaccttgcg ggttgcccgc
     3721 gatacccgtt tcatgccgga tacccaatcg ggacgtaata cccgtgattt catggtctat
     3781 cgtcttgccg gtttggccgc cgccgttatc cgagtagtag atgatcggca aaccaaaatg
     3841 cttgaccccg atacgcagag cgtccgatac cgccacacaa ctttcagcga gagagaccga
     3901 aaaaccgacc acaaaccgcg tacaaccatc aataatcacc gtcacttccg gcttaaacgg
     3961 cctgccgtgt accggatgcg ccaccttcgc cttaaagctg tggccgtcgc cgatccaaac
     4021 atcgttaggc ttcaaagccc cccaatcacg tttcacataa ggcagcagcg atttataagc
     4081 cgcccccgtt ttcctgccgc gctcctgcat aatcaacggg agtttgtccc aaacgcgccg
     4141 caccatactc aagttaggca catcgttaac cggcatattt tccgcttcag cccactgcac
     4201 aaaccggcgg tagctgtgcg ccagctttgg cgcggacgga atattgtgaa actgcataaa
     4261 catcggcaac caaccgtagc tctcaatcgg cttaaccgcc ttcgtcgtct tcggagccaa
     4321 agcaaccaac cgctccgtcg cgttttccgc tttcaaataa gcagaaatcc agccgtctaa
     4381 agtacgttcg ccaaccttcg ccgtccggct gcggtcattg gccttttcca agttcccaag
     4441 cgtgaccgcg tccaatttac cttctgccag caagcccaaa aactgagcca ccgcagcctt
     4501 ggcagagcaa tcgtattggt atttaatccc caacaccgcc gccaccaccg cacatcgcgc
     4561 atccgccacc gaccgttgtt tctcgttcaa cagcttggcc gcttcagcca gtgcttgagc
     4621 cgacatcgcc gtccccggtc tgacttgggg cagggttttc ggcatcttct ccgccagctc
     4681 gtccgactgc cgtttcatga tggctgctcg gatttcggca gggagggcat attcaacacc
     4741 accaccctta ccttgacgcc ttctcgtttg ccaaccatcc ctttgcgctt tttttctcag
     4801 ccctattact gttcttggca tatttggcaa cccaaaatta agcaggcttt tgagatccat
     4861 atattccatt tcaacccact ccgagcatct ctttcaattc agctatcttt ttgtctcgac
     4921 aatctgtttt cttgtaatgc ggaaaaactt caactaaatc tttttgaatc actttcgcga
     4981 ttgcctccgc aattcttttg cttcctcgtc catttcttat gacctcagaa acagattggt
     5041 tagttacccc taaagccgaa gccaacattc ttgccgatat atttttttct tctaaggatt
     5101 tatagatttc gttcgcttgc ataatgcctc catgatagaa tccattactt acttacctta
     5161 caggctttaa tgcctgcctt aactgttacc tggatgatat acccaaaata tttggtatac
     5221 caaatatatt tggtactatt ttgcggtgtt ttatggaacg cattgattta gcattgaatt
     5281 tagttaaaga gagaagccga cttggatact ctcgaaagaa ttttgctgag caaactggat
     5341 gcagtgcaga aagtttgaga ctgtatgaaa gcgggcaggt caacattcct gcggactttt
     5401 tacttgctgc tgtccaatta ggggtggata ttcaatttgt ctttacaggc attcattcgg
     5461 caaatttaaa tgctgtttca gacgacctta ataaaaaatt ggacgacgct attggcaaat
     5521 caaacgttga gaatcagatt aacggcaacg tcagtaacgc tgttatcgcc tcatctggtt
     5581 cggttgttca tcaaattaat acccaaaatc acgtcatcag aacccgtgtc gattcaaaac
     5641 ccggcaagga acatataacg cttgagcaag cttcaaagct gcaacaactt gttaaacaag
     5701 ttgccgcagc agaagagatt gccaaacggt cgccaaaatc aatccgtgca atttgggcta
     5761 gtctgaatgc tcactgtaaa gttccaagtt acaaactaat cgcattgagc gattacgaca
     5821 aagcagaaac ttatctcaga aaatggctgg gtaggttgag caatacagca acatcaaaga
     5881 acaacgatcc cgactggcga aaaaagaaat acgcttatat caaattaaat gtaaaacagc
     5941 ttgagcttga agattggttg aaaagttacc tcgaaaaaaa tttctcagta gaaagcctga
     6001 ctgaattaag tgatgaagat ttgcaaaaaa catatgcagc tgtatcgact aaaaaacgga
     6061 aaaaataggc ttaaacatga aactccgcct ttaaattcaa gtttcatgtt tagcttatgg
     6121 tcttcagatg ctttttttag atgtatgata ttaaaagatt ttttcaaaca tggaactatt
     6181 atacaagttc catgttcagt ttctagttaa agccttttat aaacattcta atcaaccacg
     6241 ccgaaaccga gccgcgcgac ggcctatatg tcttgcgtat aggcaatgac ctctttgtca
     6301 aacgtgtgca acgtataccg ggcaaactgc ttgtaacgtc tgaaaatcca cgttacgcac
     6361 cgtttgaaat agatttgagt aatacccaag acgacatcgc catcataggc cgtgttgagt
     6421 ggtatggccg gagtattgat taaatccatt ttaaaaacct cttaaaacag ttttaaaaat
     6481 tccctaatct cgtcagcttt aaacagaaaa ccgcgcattc cggcgcggtt ttatgaaaaa
     6541 gctggcgtaa cttttccgga tacaaaaaaa cgccgaaatc cacgtcttcc gaagatttca
     6601 gcgttttttt actctatttg ttccttgtgc aaaaactaac agtcccccac aggaaaacgt
     6661 cgctctgttc cgccacaacg gcgaaatcct gttgagcggc atcaacatca aactcaacag
     6721 cggcgattcg ctgctgatac gcggcccgag cggttgcggc aaaacctcgc tgctgcgcgt
     6781 gctggctggg ctttggccgt tcggcagcaa cggcaaagtc agccgtccgc cgcatcaaga
     6841 catcctcttc ctgccgcaac gcccgtacac ggcacagggc agcctgcgcg acgcggtttg
     6901 ttatcccgac atcgacaaac agcatccgga gctaatcgaa gccatgaaca cctgccgctt
     6961 gggttatctg gttgataaat tagataaaac cgacgactgg caacacaaac tctccccggg
     7021 cgaactgcaa cgcgtcgcct tcgtccgcgc cctgctttcc aagcccaaaa tcgtcctgct
     7081 cgacgaagcc accgccgcct tggacgaacc ggccgaagcc gcgctctacc gcgccttaaa
     7141 gcaaaaactg cccgacagca tcatcatcag catcggacac cgaagcacgc tcaacgcgtt
     7201 ccacaatatg cgcctcgatg tcggcaacgt tgcctgcggt tgaacgatgc cgtctgaagc
     7261 cgttttcaga cggcatcgtt caaattggaa aggcgcgcga aaacaacgta caataaaccc
     7321 gtttaccacc cctcggaatg ccgaaccgtg aaccagctta ttttcgactt tgccgcacac
     7381 gactatccga gtttcgacaa attcctcggc acggaaaacg cggaactggt ctatgtcctc
     7441 cgacacaaac acggacagtt tatctatgtc tggggtgaag aaggtgcggg caaaagccat
     7501 cttttgcagg cgtgggtcgc acaggcgctc gaagccggca aaaacgccgc ctatatcgat
     7561 gccgcctcca tgccgctgac cgatgccgcg ttcgaggcgg aatacctcgc cgtcgatcaa
     7621 gtcgaaaagc tgggcaacga agaacaagcc ctgctttttt ccatcttcaa ccgcttccgc
     7681 aacagcggca aaggcttttt gctgctcggt tcggaataca cgccccagca gctcgtcatc
     7741 cgcgaagatt tgcggacgcg tatggcgtac tgcctcgttt acgaagtcaa gcccttaacc
     7801 gaccaagaaa aaatcgacgc gctcgccagt atggcggcgg cacgccaagt aaccgtcgat
     7861 tccgaaattt tcgaatacct gctcaaacac tggcggcgcg atatggacag cctgatgatg
     7921 atgctcgaca cgctggacaa ctacgccgtc acaatgggca aacgcatcac cctgccgctt
     7981 ttgcgccagc ttttgaaaca acaggaaacc caatgaaaaa cctcgccatc ttcgacctcg
     8041 acaacaccct catcaacacc gattccgacc acgcctggcc gcaatacctc atcaaaaaag
     8101 gcttggttga cgctgccgaa accgaagcgc aaaacgaaaa attctaccgc gactaccaaa
     8161 acggctgcct cgacatcgac gcattcctca aattccacct cgccccgctc gcccgttaca
     8221 gcaaagaaga gctggcggaa tttcaccgcg aatttatggc ggaatacatc atcccccaca
     8281 tctcgcctat gcagcgtatg ctggtgcaga gccaccaaat ggcaggcgac gaaaccctcg
     8341 tcatctcatc gaccaacgag ttcatcatca cccccgtctg ccgccttttc ggcattacca
     8401 acatcatcgg cacacaactc gaatccggtt ccgacggccg ctacaccggc aattacatcg
     8461 gcacgcccag cctcaaagaa ggtaaaatca cccgcctgaa ccaatggctc gccgaacgcg
     8521 gcgaaacgct cgaaagctac ggcaaaacct atttttacag cgactccaaa aacgacctgc
     8581 cgctgctgcg cctcgtcgac gaacccgtcg ccgtcaaccc cgatgccgag ctggaaaaag
     8641 aagccaaaga aaaaggctgg ccggttttga atttcaaatg acgcaatgcc gtctgaagcc
     8701 cgattcggcg ttcagacggc attgaggctg aaatccaaaa aactcaaagc cacaggaatt
     8761 tatccggaaa aacaaaaaac acaggaattc atcgggaaaa acaacaatct ttccaccgtc
     8821 attcccacga aagtgggaat cttgatccgt ccgcacggaa acttatattt cgtcatttgc
     8881 acccgacata attcaatact ttattttgca ataaattaac ggatgctgac aaattagatt
     8941 ggattaatta ccctattatc catattgggt tatcgggttg ctaattgagc agagcgtagc
     9001 aaaccataaa gtgagtgttt ccacccactc tgcatttact atgaagtggt tattaaatat
     9061 gataatgaga cctattaaat ttagtatggt aaatatgtta ttatttattg ttatatgtaa
     9121 ttcatttctt gatctgctcg ttcaattatg tacaatttta tttcatagcc aaaaaatata
     9181 ctttattaca ttatttttat tatttatttt taattttgct acaaaatcta tctatatggc
     9241 aattatttat cctatttcat atttttttat cgtaaaaaat attatcctta ctctaggaaa
     9301 gtgataattc tattatcatt agcattatct atatatttta cttttatgga cttttacttt
     9361 ttttccatat attcagataa ccttagctat gaaacggagc ctttacattt atacatccct
     9421 attattatta attttttctc acttttagtt tctaatttta ttttatcttt tatcaacaag
     9481 taaaggttca gttatggaaa tttttgaggt aaatgtatat acaaaataca atccaaaaga
     9541 tcctaagcaa accaccaatc ccacaccacc cttaccggtt caaatccgca caaaaaccca
     9601 catctcgtca ttcccacgaa agtgggaatc tag
//
LOCUS       DAWHRS010000077         9628 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWHRS010000077 DAWHRS010000000
VERSION     DAWHRS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.077"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            2..202
                     /locus_tag="WIB82_001297"
     CDS             2..202
                     /locus_tag="WIB82_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842592.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
     gene            370..905
                     /locus_tag="WIB82_001298"
                     /pseudo
     CDS             370..905
                     /locus_tag="WIB82_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            938..1129
                     /locus_tag="WIB82_001299"
     CDS             938..1129
                     /locus_tag="WIB82_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842593.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            1187..1843
                     /locus_tag="WIB82_001300"
     CDS             1187..1843
                     /locus_tag="WIB82_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ3842594.1"
                     /translation="MMKFKYVFLLACVVVSLSYRLNAAPMFNDNPVVYGKIKVQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVLEKLDKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLGHVSM
                     SRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFTHHPKRQTEFAVGQIRQARAE
                     "
     gene            1970..3265
                     /gene="hisS"
                     /locus_tag="WIB82_001301"
     CDS             1970..3265
                     /gene="hisS"
                     /locus_tag="WIB82_001301"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ3842595.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNHWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIRDYLTLEINSLGKREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            3266..3895
                     /locus_tag="WIB82_001302"
     CDS             3266..3895
                     /locus_tag="WIB82_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ3842596.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            4045..5502
                     /gene="der"
                     /locus_tag="WIB82_001303"
     CDS             4045..5502
                     /gene="der"
                     /locus_tag="WIB82_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ3842597.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEEIL
                     ENFPEPEVEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     ERKGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            5533..6432
                     /gene="rdgC"
                     /locus_tag="WIB82_001304"
     CDS             5533..6432
                     /gene="rdgC"
                     /locus_tag="WIB82_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ3842598.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAASLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            6484..6900
                     /locus_tag="WIB82_001305"
     CDS             6484..6900
                     /locus_tag="WIB82_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842599.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYDGGVGKDRLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            6966..7532
                     /locus_tag="WIB82_001306"
     CDS             6966..7532
                     /locus_tag="WIB82_001306"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ3842600.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(7596..8300)
                     /locus_tag="WIB82_001307"
     CDS             complement(7596..8300)
                     /locus_tag="WIB82_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ3842601.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEAVQDTMSA
                     RLQVAAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAILRFK
                     ARADKLSGILGTSGYKIVKLNLGHIGSHIAGEGAAQAKMLRAMPMAASYGVEGTDSAA
                     PGVEEISISVNGTVQF"
     gene            8669..9052
                     /locus_tag="WIB82_001308"
     CDS             8669..9052
                     /locus_tag="WIB82_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3842602.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTKHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            9084..>9628
                     /locus_tag="WIB82_001309"
     CDS             9084..>9628
                     /locus_tag="WIB82_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3842603.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIP"
BASE COUNT         2611 a         2443 c         2303 g         2271 t
ORIGIN      
        1 aatgcaattg atatgtgccg attggacagg tataggaaat tttatgaaaa ccttcgaaaa
       61 aacatggtct gcacaatatc gggatatgga aatttcagta cggaattttt ggaatttgga
      121 gcggacaggg gcggaagtct atatcaacgg aaggcgggtt tatcataacg aagccgaaat
      181 ggcgtctgct tctttgcgtt agctaatggg ggaatacctg gaatttgaag aaagcggtac
      241 gaaaattacc gttgaaatcg gcagcgcgtg gcattttaat gaaactataa gaaatattaa
      301 tacacattta agaggtacga aacttccagg ttctaactgg agagttcaag ataaggggca
      361 ttttcatctt tggaaaagat aatagattct aaggtaaggg agactaattt taatcaggca
      421 tggcgtgtta tacgcgccat gcaaatagat aatatatccg atgaacaggt tgtgattgtc
      481 agaatgtctt gttatcttaa gaaagatgaa atttttaaga atatagaaag atatggatat
      541 cagtcatcag atattaagaa aattagttct ccttttccat taaatatttt acctgctccc
      601 aaaccttatt atttttatct ttatgatacc catttgaatc aaatttatga tttggcttgg
      661 tcatgtaatg attttttaat gtttgaaata tttattttgg atagggaaag catcaaatat
      721 atagataaag ataaactttt taaatctgga gaaacactat attcgaattt taaatctatt
      781 aaaaatcaat gcaaaaatta tttttttgcg gttttgattt tgatgcccct tactttaaaa
      841 gcggtgtgag tcaggtggca gattataatt ttgttcctga tgcattttct ttattcttta
      901 tttaatcaaa ttatttgaat aagcgtaggg tgggcacttg ctgcccacgc gttctgtttc
      961 aagtttatta ttgggtgaat atttggaatt tgaagaaaac ggtacaaaga ttgctgcgga
     1021 aatcgacagt gcatggcatt ttttgggcgc ggcttgtcgg atttcaataa atggaaagta
     1081 ttatgcgggt aatcggattg tttggtttgc caaaaaagcc gaaagctagg aataatttga
     1141 caagggcagc ctgaaagatt ttcaggctgc cctttatgtg gacaatatga tgaagttcaa
     1201 atatgttttt ctgttggcgt gtgttgtcgt ttctttatct tatcgtttga atgctgcacc
     1261 gatgtttaac gataatcctg ttgtttacgg aaaaatcaaa gtgcagagtt ggaaagcgcg
     1321 gcgggatttc aatattgtaa agcaggattt ggatttttcc tgcggggcgg cttcggtggc
     1381 gacgcttttg aacaattttt acgggcaaac gctgacggaa gaagaagtgt tggaaaaact
     1441 ggataaggag cagatgcgcg cgtcgtttga ggatatgcgg cgcattatgc ctgatttggg
     1501 ttttgaagcg aaaggctatg ccctgtcttt cgagcagctt gcacagttga aaatccccgt
     1561 catcgtgtat ctgaaatacc gcaaggatga tcatttctcg gtattgcgcg gtatagacgg
     1621 caatacggtt ttgcttgccg acccgtcgct ggggcatgtt tcaatgagca gggcgcagtt
     1681 tttggatgct tggcaaaccc gtgagggaaa tttggcaggt aagattttgg cggtcgtgcc
     1741 gaaaaaagcc gagacaattt caaataaatt gtttttcaca catcatccca agcggcagac
     1801 agagtttgca gtcggacaaa tcaggcaagc acgtgcagag taagtcaaat gccgtctgaa
     1861 aactgtttca gacggcattt ttacccgtcc gcaaaccacc cccaacctca caagccctta
     1921 aaaatccgct ataatcgccc gcaatttgat ttaagcattc ttacttgaaa tggcacaaaa
     1981 aatccaatcc gtcaaaggca tgaacgacct tctgcctgtc gagcaaaaag atttcaaact
     2041 gacggctgcg ttttggcagg cgtttgaaga tacggtaaac cattggacgc gcgcttacgg
     2101 ctatcagcaa atccgtacgc cgattgtcga gcaaaccggt ttgtttgtcc gctctatcgg
     2161 cgaggaaacc gatgtggtcg gcaaggaaat gtacaccttc tccgattcaa acgattcttt
     2221 gagtttgagc ttgcgtcctg aaggtacggc ttcttgtctg cgtgcggtgg tcgaacacaa
     2281 ccttctgtac aacagcccgc aaaagctgtg gtatatgggg ccgatgttcc gccgcgagcg
     2341 tccgcaaaag ggccgttatc gtcagttcca tcaggtcggt atcgaggctt tgggttttga
     2401 agggccggat attgatgcgg aaatcatcgc gatgtctgcc gacttatggg aaaaattggg
     2461 tatccgcgat tacctgactt tggaaatcaa cagcttgggc aagcgtgagg aacgcgcggc
     2521 acaccgtgcg gcattggttg aatatctgac ccgttatgaa gataaattgg atgaagacag
     2581 caaacgccgt ctgaaaacca atcctttgcg cgttttagat acgaaaaacc ctgatttgca
     2641 ggaaatctgc aacgccgcgc cgcgtttggt ggattatttg ggcgaggctt cgcaaaacca
     2701 ctatgcacgc ttcaaagcga tgttggacgg tttgggtatc caatatattg aaaatccgcg
     2761 cttggttcgc ggtttggatt attacaatca aacggttttt gagtggacga ccgacaaact
     2821 cggcgcgcag gcaactgtgt gcggcggcgg ccgttacgat ggtttgattg aggaacttgg
     2881 cggcaaacct gcgccgtcca tcggctttgc gatgggcatc gaacggctgc tgcttttggt
     2941 gagcgaatac ggctcgctgg aagtgaacgc tgcgcctgat gtctatgcaa tgcaccaagg
     3001 cgaaagggcg gacttgcagg tgatgaaata cgcgcaagcc ttgcgcgcgc aaggtttcaa
     3061 tgtaatgcag cattccggct atcaaagcct gaaagcgcaa atgaaaaaag ccgacaacag
     3121 cggcgcgcgc tttgccctga ttgtcgcgca agacgaactg gcgaacggta cggttacgct
     3181 caaagacatg aacggcgcac acggtcagca aactgtcgcc gccgaggatt taacccatac
     3241 tttacaacaa tggaagaacg cataaatggc agcccatctc gaagaacaac aagagttaga
     3301 caactttaaa tatttttgga aaaccacggg caaatggctg tttgccctgc tgattttggc
     3361 agcactcggc tacttgggat acacggttta ccaaaaccgt gcggcttccc aaaatcagga
     3421 agcggcggcg gtgctggcaa acatcgtgga aaaggcgcaa aacaaagccc cgcaaagcga
     3481 aattaatgcc gaattgacca aactccaaca aagctacccg cattctattt ccgccgccca
     3541 agccacgctg atggcggcgg caaccgaatt tgacgcgcag cgttacgatg ttgccgaagg
     3601 ccatttgaaa tgggtgttgt ccaaccaaaa agacagcctg attcaagcgt tggcggcgca
     3661 gcgtctgggc gttgtgttgt tgcaacaaaa aaaatacgat gccgcgcttg ccgcgctcga
     3721 cacgccggtt gaagcggact tctcccccct gctgatggaa accaaaggcg atgtctatgc
     3781 cgcacaggga aaaagccagg aagccttaaa aaactacggt caggctttag aaaaaatgcc
     3841 tcaagattct gtcggtcgtg aattggttca aatgaaactc gattcgctga aataaatgcc
     3901 gtctgaaatg ccaccctctc ttcagacggc atttcagaca acactatcct acggcaattc
     3961 ccgtaaaacc atctgccgtg tcccaacgga cacctgctcg tgttcagttc tcccgaacgg
     4021 cacttgacac agaaagactc aatcatgaaa ccaaccatcg cgcttgtcgg ccgccccaac
     4081 gtcggcaaat ctactttatt caaccgtttg acgcgcacca aagacgcgct cgtgcatgac
     4141 ctgcccggcc tgacgcgcga ccgccattac ggacacggca aagtcggcag caaaccttat
     4201 ttggtcatcg ataccggagg ttttgagccg gttgtggata gcggcatttt gcacgaaatg
     4261 gcaaaacaaa ccttgcaggc tgtcgatgaa gccgatgccg tcgtattttt ggtggacggc
     4321 cgcaccggtc tgaccccgca agacaaaatc attgccgacc gtttgcgcca aagcccgcgc
     4381 cctgtttatc tggcggtaaa caaaggcgaa ggcggcaaca gggccgtgct tgccgccgag
     4441 ttctacgaac ttgctttggg cgacccttat gttatttccg gtgcgcacgg cgacggcgtg
     4501 tattacctga ttgaagaaat tttagaaaac ttccccgagc cggaagtcga agaagccgat
     4561 gcaaaacatc ccgtttttgc cgtcatcggc cgtccaaacg tcggcaaatc tacgctggtt
     4621 aacgccattc tcggcgaaga gcgtgtgatt gcattcgata tggcaggtac gacacgcgac
     4681 agcatccaca ttgattttga acgtaaaggc aaaccgttta ccatcatcga taccgcaggc
     4741 gtgcgccgtc gcggcaaagt cgatgaagcg gtggaaaagt tctccgttat caaagccatg
     4801 caggcggttg aagcggcaaa cgtcgctgtt ttggtattgg acgcgcagca ggacatcgcc
     4861 gaccaagatg cgacgattgc aggtttcgct ttggaggcag gccgtgcttt ggtggtggcg
     4921 gtcaataaat gggacggcat cagcgaagaa cgtcgcgagc aagtgaaacg cgatatcaac
     4981 cgcaaactgt atttccttga ttttgctaaa ttccacttta tttccgcatt gaaagaacgc
     5041 ggcatagacg gattgttcga cagcattcag gctgcctaca acgcggcgat gattaagatg
     5101 ccaacaccga aaatcacgcg cgtattacaa agcgcgatcg agcgtcagca accgccgcgt
     5161 gccggcttgg tgcgtccgaa aatgcgttat gcccaccaag gcggcatgaa ccctcccgta
     5221 attgtggtac acggcaattc gctgcacgcg atttccgaca gctatacgcg ctatctgacc
     5281 caaacgttcc gcaaagcctt caatctgcaa ggcacgccgc tcagaattca atacaatgtt
     5341 tcggaaaacc cgtatgaaaa tgcggaagac aaaccgaaga aaaaaccgct gcgccgcgtc
     5401 agcctgagca accgtattga gaaacgcgaa ggccgaaagg aagagaaaaa ccgcttcaag
     5461 aagaaaacca aagtcagtgt aaaaaaacaa ttcagcaaat aattcccgac atttcaaaca
     5521 ataggaaaca ttatgtggtt caagcagatt agtttttatc cgctcaacaa agaaaagctg
     5581 cctgaggcag acgtacttgc cgacaaactt gctgaagctg aatttaccca ttgccaaggc
     5641 ttagactggt tcagcgaagg ctttaccgca ccggtttcgt tctctcccga actcgttttc
     5701 cctgccgact ttaccttgcg cgtcgccctg aaaaaagaag aaaaagtcct gcctgccggc
     5761 gtcatccgcg atattttgga agagaaggta gcggaaatcc aaaacaatga agcccgcaat
     5821 gtcggtcgca aagaaaaaca agagcttaaa gagcaaatta cagacgacct gctgccccga
     5881 gcgtttaccc gcagcagccg tacagaagcg gtgtttaaca cccgccacgg ctacctgctc
     5941 gtcaataacg cggcttccgc caaagcagaa aacatcctga ccaagctgcg cgaagctttg
     6001 ggcggtttgg aagcctcgct gccgaatacc aaacaatcgc cctcttctct gatgaccggc
     6061 tggctgttgc aagggcattg cgaaggcggt tttgaattgg acagcgattg cgaactcaaa
     6121 ggtacgggcg atattgttcc cgtcgtcaaa gtatccaaac aagatttaac cgccgacgaa
     6181 gtggttcaac acgtcaaaaa cggtaaaacc gtaacccagc tcggtttggt ttggcgcgaa
     6241 caaatcgcct ttatcctcac tcaagacttc acactcaagc gcatccaata cctcgacgta
     6301 ttgcaggaag aagccgaaag caacggcgac gatgccgcca gccttgcctt cgcctcgcaa
     6361 atcctgatgg ctgaatccgt cagcaccatg ttggaagagc tggtttccta tttgggcggc
     6421 tggcaagatt gatttttcag acggctttta taccgtcagc caaaatccaa tggaataacc
     6481 cttatgctta aacatctcgc attcctactg cccgccatga tgttcgccct ccccgccgcg
     6541 tccgccgttc tgacttccta tcaagaaccc ggctgcacct acgacggcgg agtcggaaaa
     6601 gacaggcttc cttcaggcaa aggcatatgg cgttgccggg acgggcgcgg ttataccggt
     6661 tcgtttaaaa atggcaaatt cgacggacaa ggcgtttata ccgttgccgc caaccgcgaa
     6721 atatttatcg aaccgttcaa ttccgacagt accaaattcc gcaacatggt actctcgggc
     6781 acgttcaaaa aaggcttggc acacggcaga tttaccgtct cgcaaaacgg cgaaaccctc
     6841 ttcattatga aatgcgaaaa cggcatgatt aaagaagtga agctgcccaa aaacaaataa
     6901 gccccgtttt cagacggcat cccccaatgc tttgtccgaa cactgcaaga aacaaggaaa
     6961 acattatgag catcaagtcc gacaaatgga tacgccgcat gagcgaagaa ttcggcatga
     7021 tcgacccttt cgagccgaac caaatcaaag aagccgacgg caaacgcatc atctcctacg
     7081 gtacgtccag ctacggctac gacatccgct gcgccaacga atttaaaatc ttcaccaaca
     7141 tcaacagcac catcgtcgat cccaaaaact tcgacccaaa aaacttcgtt accgttgaag
     7201 acgactgctg catcatcccg cctaattcct tcgcactggc gcgcacggtc gaatatttcc
     7261 gcatcccgcg caacgtcctg accgtctgct tgggcaaatc cacctacgcc cgctgcggca
     7321 tcatcgtcaa cgttaccccg ttcgaaccgg aatgggaagg ctacgttacc ctcgaatttt
     7381 ccaacaccac ccccctgccc gccaaaatct acgccggcga aggcgtggcg caagtcctct
     7441 tcttcgagag cgacgaaatc tgcgaaacct catacaaaga ccgcaacggc aaatacatgg
     7501 gacaaaccgg cgtaaccctg cccaaagcct gaaatataaa tgccgtctga acccttatcg
     7561 tcgggtttca gacggcattt gccgttcacc tcaaatcaga actgaaccgt cccattgacg
     7621 ctgatgctga tttcctccac acccggcgcg gcggaatccg taccctccac gccgtaactt
     7681 gccgccatcg gcatggcacg aagcattttt gcctgagcag ctccttcacc cgcgatatgg
     7741 ctgccgatgt gtcccaaatt taatttgacg attttataac cggacgtacc caatatgccc
     7801 gacaacttgt ctgcacgcgc cttgaaacgc aaaatagcat ctttactgac ctgatcgatg
     7861 acctcgtttc ggcgttcgcg cgacacatgg aaatccgtat attccaatgc ggcatctgcc
     7921 tgaacatcgg caataaaacg gtttaactca tcaaaatccc tcccttcgac cttaaattcc
     7981 gcacgctcct cccaacctgt ttgaatgcgt ctgccgttgg tatattgata gcgcggcatc
     8041 gcactgcgcg ataccaattc ggttttaaag ctaccatttt tcgattttct gatgaacttg
     8101 ttgaattttt taacaaactc ggcattgacg gcatttttgt cccgtccttc cgccgccacc
     8161 tgtaaacgcg cggacattgt atcctgaacc gcctcgacac cggccgattc ggaaaattcg
     8221 acaatattgt aattcaatgc ttcagccgct gccggaaaag ataccgccaa tagggaagcc
     8281 gccaaaatag aacgcagcat atccgtatcc ttacttataa aaccgccaat ctagcatatc
     8341 ggcgcgctaa attgcgttag cgtaaggcaa caatttaaat attcggtctg cgtctgtctt
     8401 ataataaccg cctttccgat acggaatccc actatgccca aacccttgca cgtcctcgtc
     8461 atcccctcat ggtatccgca atccgaacag gatgtggacg ggattttttt caaaatcagg
     8521 cactggcatt gcagaggaaa ggcatcaaaa ccgccgtgct tgcaccgatg ttccgctact
     8581 tgcggaaaga aacagcaagc atcctgaccg gccttacggt tttgcaaaat atcggcaaag
     8641 cggtttggac atctatgcat ggcacggcat atatttcttc ccccgctttc cgttcatcga
     8701 catcgactgc atccgctggg tgtgtgcggg tttgaaagct ttcaaacact acatccgcga
     8761 aaacgggctg cccgacctga ttcacgccca ccgcatgaac tatgccggca tactcgccca
     8821 aaaaatttcc caaaaatacg gcatccccta tgtcgtcacg aaacacagca gcaccatcac
     8881 gcgcggtctg atccgtcccc accaatggca gccgatggaa aaagcagcgg cgcacgcctc
     8941 cgcactgctc gctgtcagcc gccatttcgc acacgtcctg caacacaaat acggctgcga
     9001 atggcagtac ctcccccaac atactgggcg gaatattcaa ccgaccgttt gaacaaagag
     9061 aaaaaaacaa caaaccgcat ttcgtgttct gcaccgtctc gcaccttcgc cgtctcaaag
     9121 gacacgatgt cctactcact gcctttgccc gggcgttggc acaatgcccg caactgcgcc
     9181 tgaacatcgg cggcagcgga caggaagaac agcggctgaa acagcaggcg gcagacttgg
     9241 gcattaccca tgccgttaca tttttgggcg cattgcagcc cgaagcagtc ttggatttga
     9301 tgaggaacag cgacgcattc gtccttgcca gccgcaccga aaccttcggc gtggtctata
     9361 tcgaagcact gtcccaagga ttgcccgtca ttgcgacacg ctgcggcggt gcggaatcta
     9421 ttgtttcaga cggcaacgga tatttggttc ctgttgacga cgacgatgcc cttgccgacg
     9481 cactcatcaa aatgtatgaa caccactctg attttgaacc tgcccgactt agggaaaact
     9541 gtctgaacga atttggcgaa aatgccgtta taggcaggtt gatcggcatt ttccgacagg
     9601 caatcgcaga atacggtaag aaaatacc
//
LOCUS       DAWHRS010000078         9557 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWHRS010000078 DAWHRS010000000
VERSION     DAWHRS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.078"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1755)
                     /gene="dxs"
                     /locus_tag="WIB82_001310"
     CDS             complement(<1..1755)
                     /gene="dxs"
                     /locus_tag="WIB82_001310"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ3842604.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSENKPAAKPTYTQVF
                     GKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLACE
                     GMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSFLR
                     CVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKGII
                     RREGGKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVTLE
                     ENAEQGGAGGAVLEVLAKH"
     gene            complement(1840..2757)
                     /gene="xerC"
                     /locus_tag="WIB82_001311"
     CDS             complement(1840..2757)
                     /gene="xerC"
                     /locus_tag="WIB82_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ3842605.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALSQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            2951..4015
                     /locus_tag="WIB82_001312"
     CDS             2951..4015
                     /locus_tag="WIB82_001312"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ3842606.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            4035..4994
                     /locus_tag="WIB82_001313"
     CDS             4035..4994
                     /locus_tag="WIB82_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ3842607.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPDGKAEYHG
                     KAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPEQNVELAAAELKADEKSHAVILGDT
                     RYGSEEKGTYHLALFGDRAQEIAGSATVKIGEKVHEIGIAGKQ"
     gene            5133..5810
                     /gene="tsaB"
                     /locus_tag="WIB82_001314"
     CDS             5133..5810
                     /gene="tsaB"
                     /locus_tag="WIB82_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ3842608.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETHLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYTKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            5807..6247
                     /gene="rimI"
                     /locus_tag="WIB82_001315"
     CDS             5807..6247
                     /gene="rimI"
                     /locus_tag="WIB82_001315"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ3842609.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            6241..6978
                     /locus_tag="WIB82_001316"
     CDS             6241..6978
                     /locus_tag="WIB82_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ3842610.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            7040..7681
                     /gene="pyrE"
                     /locus_tag="WIB82_001317"
     CDS             7040..7681
                     /gene="pyrE"
                     /locus_tag="WIB82_001317"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ3842611.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            7755..8189
                     /locus_tag="WIB82_001318"
     CDS             7755..8189
                     /locus_tag="WIB82_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842612.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVAVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            8186..9496
                     /gene="argA"
                     /locus_tag="WIB82_001319"
     CDS             8186..9496
                     /gene="argA"
                     /locus_tag="WIB82_001319"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ3842613.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTL
                     AVTLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
BASE COUNT         2100 a         3031 c         2546 g         1880 t
ORIGIN      
        1 gtgtttcgcc aatacttcca gcaccgcgcc gcctgcgccg ccctgttcgg cgttttcttc
       61 aagggtaacg atgcggtcgt ggcttcgggc aaggcggaca atcaactctt cgtctatcgg
      121 tttgacaaag cgcatatcgg cgacggtggc gttcagtttt tcggcaaccg ccaatgcggg
      181 ggcgaccata ctgccgaagg caatgaatgc ggttttccca ccttcgcggc ggataatgcc
      241 cttgccgatt tccacggttt ccatgccgtc tgaaaccggc gcgcccgtac ccgtgccgcg
      301 cggatagcgg acggcggcgg gcgcgtctgc ctgatagcag gtcgaaagca acaggcggca
      361 ttcgttttca tcgctcggcg cggcgacaat catgttcggc acgcagcgca aaaagctcaa
      421 atcgtacaga ccggcatggg tcgggccgtc cgcgccgacg atgcccgcgc ggtcgacggc
      481 aaacaaaacg ggtaggtttt gcagggcgat gtcgtgcacc agttggtcgt aggcgcgttg
      541 taaaaaggtg gaataaatcg ccacgacggg cttcatccct tcgcaagcca aaccgccggc
      601 aaaggtaacg gcgtgctgct cggcaatgcc gacgtcgaaa tagcggtcgg ggaatcgttg
      661 ttcaaactca accaagccgc tgccctcgcg catggcgggg gtaatcgcaa ccagtcggga
      721 atctgccgcc gcccggtcgc acagccattt gccgaacact tgggtatagg tcggtttggc
      781 ggcaggtttg ttttcagacg gcatttgcgc cgcgctttct ttgggcaggt tggcgacggc
      841 gtggtatttg acggggtcgt tttcggcaag tttgtagccg ttgccctttt tggtgatgac
      901 gtgcagcagt tgagggcctt tgcgactgcg caagtctttc aatacgtcca ccagattttc
      961 gacgttgtgt ccgtccacgg ggccggtgta gcggaagccg aagttttcaa acaaagacag
     1021 cgactgtttg gcgtgttcgg cttcttcggc aagggttttg attttgtgtt cgactttttg
     1081 ggcaaactcc atcgcgccgg gtattttgtc taataccttg cccgtttgcg ctttgacggt
     1141 actcaacagg ccgtgcatat cgcgcacgac gttgctggca aggtatttcg gcagcgcgcc
     1201 gacgttgggg gaaatcgaca tttcgttgtc gttgaggacg accagcaaat ccacatccat
     1261 atcgcctgcg caattcaagg cttcaaacgc ctgccccgcc gtcatcgcgc cgtcgccgat
     1321 gatggcgacg ctgcggcggt cgctgcccaa gagtttgtct gccgccgcca tgcccaacgc
     1381 cgcgccgatg gaggtggagg aatgccccac gccgaacgcg tcgtactcgg actcgcaacg
     1441 tttcggaaaa cccgccaaac cgccgtattg gcgcatggtg tgcatctggt ttttgcgtcc
     1501 ggtaaggatt ttgtgcggat agctttggtg tccgacatcc cacaccagct tgtcttcggg
     1561 cgtgttgtac acatagtgca gggcgatggt cagctcgacc gcacctaagt tgctggcgaa
     1621 atgaccgccg gtttgaccga cggattccag cagaaaggcg cgcaactcgc cggcaaggcg
     1681 cggcagctgt tttttgtcca gacggcgcaa atcttgcggg ctgtcaatca ggtcgagtag
     1741 ggggcttggg ttcatggtgt gtctttttta tgtgtcgtcc gggtgcaacg gccaattata
     1801 tatcaagagt gtgcggctga cggctgattt tgccgtatgt cattcgtcct gccgcttggc
     1861 gcgcgggtgg gcttcgtcat acaggcgggc gatgtggtcg aaatcgagtt tggtgtaaat
     1921 ctgcgtggtc gaaaggctgc tgtgcccgag cagctcctgc accgccctga tgtcgcgcga
     1981 agcctgcaac aggtgtccgg cgtagctgtg gcgcatcata tgcggcgaaa cgtgcctgcc
     2041 gtcgccgttt tgcgccgccc attgcgccaa acgtttttgg atttggcgtt ggctcaggcg
     2101 cgtgccgttc ctgccggtaa acagggcttt gccgtccgat gccgtctgac gcagcggcag
     2161 atagtttttc agggcttcca cgcttttgcc ggtcagcggc acctgccgct gcttgcgccc
     2221 tttgccggta acgtgtaccc acgcctcgtc caaatagaca tcatctgcat tcaagccgtg
     2281 tatctcgctc acgcgcaaac cgctgccgta catcagctcg aacagggcgt gatcgcgcac
     2341 cgccagcggg tcgccgccgt ccacgggcaa atccagcatc cggttcagcc attcctgcga
     2401 cagggctttg ggtacgcgct cgggctgctt cggcggtttg atgtcggcgg tcgggtcggt
     2461 atgcagcaga ccgcgttcca ccagccaaac acaatactgc cgccaagacg acagcttgcg
     2521 cgccagcgtc cgcgcattca aaccgcgctg cgacagccgc cgcaacgccg ccgtaaaatc
     2581 gcgccgcgac aagtcctgcg gcacgccgcc tgcatcttca gacggcattt gtgccaacag
     2641 cgcaaacagt tcttccaaat cgcgccggta tgccgccacc gtgtgctccg acttgccctc
     2701 gcgcacgatg ttttccaaat aagcgtcaaa atgcgccgca aacccgtcca aaaccataac
     2761 cgccccacac aaatatcaaa aaaacagtga aaacattatc ataaacgaca caccaaaacc
     2821 ccgaaccgcc caaaatccga cccgtccgcc cgccgttatc gttaacataa aatcaatgaa
     2881 ccgtttatgg ttttttgccg caaaaaacat tataatccgc cttatttacc tattgcccaa
     2941 ggagacacaa atggcactcg tatccatgcg ccaactgctt gatcatgctg ccgaaaacag
     3001 ctacggcctg cccgcgttca acgtcaacaa cctcgaacaa atgcgcgcca ttatggaagc
     3061 cgccgaccaa gtcaacgcgc ccgtcatcgt acaggcgagt gcaggtgcgc gcaaatatgc
     3121 gggcgcgccg tttttgcgcc acctgattct ggcggcagtc gaagaatttc cgcacatccc
     3181 cgtcgtgatg caccaagacc acggcgcatc gcccgacgtg tgccaacgct ccatccaact
     3241 gggcttttcc tccgtgatga tggacggctc gctgatggaa gacggcaaaa ccccttcttc
     3301 ttatgaatac aacgtcaacg ccacccgtac cgtggttaat ttctcccacg cttgcggcgt
     3361 atccgtcgaa ggcgaaatcg gcgtgttggg caacctcgaa accggcgaag ccggcgaaga
     3421 agacggtgta ggcgcagtgg gcaaactttc gcacgaccaa atgctcacca gcgtcgaaga
     3481 tgccgtgcgt ttcgttaaag ataccggcgt tgacgcattg gcgattgccg tcggcaccag
     3541 ccacggcgcg tacaaattca cccgtccgcc cacaggcgac gtgttgcgta tcgaccgcat
     3601 caaagaaatc caccaagccc tgcccaatac acacatcgtg atgcacggct ccagctccgt
     3661 tccgcaagaa tggctgaaag tcatcaacga atacggcggc aatatcggcg aaacctacgg
     3721 cgtgccggtt gaagaaatcg tcgaaggcat caaacacggc gtgcgtaaag tcaacatcga
     3781 taccgacttg cgccttgctt ccaccggcgc ggtacgccgc taccttgccg aaaacccgtc
     3841 cgacttcgat ccgcgcaaat atttgagcaa aaccattgaa gcgatgaagc aaatctgcct
     3901 cgaccgctac ctcgcgttcg gttgcgaagg tcaggcaggc aaaatcaaac cggtttcctt
     3961 ggaaaaaatg gcaaaccgtt atgccaaggg cgaattgaac caaatcgtca aataacaggt
     4021 tgcttgtaaa caaaatgccg tctgaaccgt tgttcggacg gcatttgatt tttgcttctt
     4081 tgacctgcct cattgatgca gtatgcaaaa aaagatacca taaccaaaat gtttatatat
     4141 tatctattct gcgtatgact aggagcaaac ctgtgaaccg aactgccttc tgctgccttt
     4201 ccctgaccgc cgccctgatt ctgaccgcct gcagcagcgg aggcggcggt gtcgccgccg
     4261 acatcggcgc ggggcttgcc gatgcactaa ccgcaccgct cgaccataaa gacaaaagtt
     4321 tgcagtcttt gacgctggat cagtccgtca ggaaaaacga gaaactgaag ctggcggcac
     4381 aaggtgcgga aaaaacttat ggaaacggcg acagcctcaa tacgggcaaa ttgaagaacg
     4441 acaaggtcag ccgcttcgac tttatccgtc aaatcgaagt ggacgggcag ctcattacct
     4501 tggagagcgg agagttccaa atatacaaac aggaccactc cgccgtcgtt gccctacaga
     4561 ttgaaaaaat caacaacccc gacaaaatcg acagcctgat aaaccaacgc tccttccttg
     4621 tcagcggttt gggcggagaa cataccgcct tcaaccaact gcctgacggc aaagccgagt
     4681 atcacggcaa agcattcagc tccgacgatg ctggcggaaa actgacctat accatagatt
     4741 tcgccgccaa acagggacac ggcaaaatcg aacacctgaa aacacccgag caaaatgtcg
     4801 agcttgccgc cgccgaactc aaagcagatg aaaaatcaca cgccgtcatt ttgggcgaca
     4861 cgcgctacgg cagcgaagaa aaaggcactt accacctcgc ccttttcggc gaccgcgccc
     4921 aagaaatcgc cggctcggca accgtgaaga taggggaaaa ggttcacgaa atcggcatcg
     4981 ccggcaaaca gtagcatttg aaaaatgccg tccgaacacg ataatttacc gttcggacgg
     5041 catttttgcg ccataccgcc ctaggatacg acggcacggc aggcaagccg gtttcactat
     5101 ataatgccgt ctgaaccaac gagaaccata ccatgcaagc tgattttaac cgtcccgtcc
     5161 tggccatcga taccggtact tcctatttgt cgctcgcgct gcgcgccgac ggcgaaaccc
     5221 acctgttcca tcaagaggcc ggcagccgcc aatccgaact gatcctgccg gaaatccgca
     5281 ctctattccg aaatgcgggc attaccgccg ccgatttggg ggcgattgtt tatacgaaag
     5341 gtcccggcgc gtttaccgga ctgcgtatcg gcatcggtgt ggcgcaaggt ttggcaacgc
     5401 cgtttgatac ccctttaatc ggcatcccca cgctcgatgc cgccgcctcg ctgccgccgc
     5461 cgcaaagctg catactcgcc gctgcggacg cgcgtatggg cgaagtgttt tatgcgtggt
     5521 tcgacacgct gaaccgccgc cgtttgagcg attatcaggt cgggcgggcg gcagacatcc
     5581 ggctgccgga gggatgtgcc ttttcagacg gcataggcag cgcgttcgcg ttggaagaaa
     5641 cgccgccgtt ttcaggcaaa cccgatatgc cgaccgccgc cgacttcctc aatctggcgg
     5701 cggaaggcgg ttttcccgcc gtcgctgctg cacacgccga actgctctac gtccgcaaca
     5761 aaatcgccct aaccgccaaa gaacaggcgg aacggaaagc ccgaacatga acatccgccg
     5821 cgcctcaccg tccgactgcg ccgcactcgc cgcgctcgat gccgtctgca acccgtccgc
     5881 gtggacgcaa cgccaatttg agtccgcatt ggtttcgccg tccgaacagg ttttcctcgc
     5941 ggaaaaagac ggcgggattg ccgcctttat cgtttggcag aacctgcccg acgaatccga
     6001 actgcacctg attgccaccg cgcccgaatg ccgccgccgt ggcgttgcgt ccgccctgct
     6061 cgaatattgg ttcgcacatc tgcccgaaga cacgcaacgc ctgctgctcg aagtccgtgc
     6121 aggcaacacc gccgcacagg cactgtacgc ggcgcacggc tttaccgatg cgggcaggcg
     6181 gaaaaactat taccgtacag ccgacggtaa aaccgaagat gctgtcttaa tggagaaaat
     6241 atgttaagcg cgcgctacct ccacctgcac gaagctttgg gtttaggccc gatgtggctg
     6301 aaacaggccg ccgccgtcct gccgcccaaa aacacacccg caaccccggc acaggcacgt
     6361 cctcaaaccg tccgcgccgc cccgatccgc ccttcccaac cccataacgg tcaggcgcgg
     6421 ctcgaaacga tgaaagcgtt ggaaaccgcc gccgtacata cgcgcaaacc cgcgcctgaa
     6481 accgaaacgc ccccgcccgg cctttcagac ggcatcgccc ccgttcccgc cgcttcgggc
     6541 ataaccaaac ttgccgtcgt cagcctttgc ccaccgattg aggatgcggt ttacgggcaa
     6601 ctgttctacg gcaaagcggg tgttctgctt gacaacatac tcaaagccgt aggactggat
     6661 gccgcctatg tccacaaaac ctgttgggtg aaaaccgccg ccgtcggcaa cccgatgccg
     6721 tctgaagcgg caatcgaaac cgcgacggtt tccgtcatgc aggaactcga cggctgccgc
     6781 gcccccgccg tcctattcct cggacaggct tttgtccagc ctgaacggca aacgatgatt
     6841 gaaactttgt gcggcagccg tcccttcttc atcatcgacc atcccgcccg gctgttgcgc
     6901 caacccgaac ttaaagcccg cgcctggcag gtgttgaaac agttgaaacg cgccttgcgg
     6961 caaggcggcg gcagttgaag cgcaccgcac ggggcggtag aatcgcaact gcgtcccaat
     7021 atctgacaga aagcacaaaa tgaccgattt ccgccaagat ttcctcaaat tctccctcgc
     7081 ccaaaatgtt ttgaaattcg gcgaatttac caccaaagcc ggacggcggt cgccctattt
     7141 cttcaatgcc ggcctcttta acgacggctt gtccacgctg caactggcaa aattttacgc
     7201 acaatccatc attgaaagcg gcatccgatt cgatatgctg ttcggccccg cctacaaagg
     7261 cattattttg gcggcggcaa ccgcgatgat gctggcggaa aaaggcgtga acgtcccgtt
     7321 tgcctacaac cgcaaagaag ccaaagacca cggcgaaggc ggcgtgttgg tcggtgcgcc
     7381 gcttaaaggc cgcgtgctga ttatcgacga cgtgatttcc gccggcacat ccgtacgcga
     7441 atcgatcaag ctgattgaag cggagggcgc aacccccgcc ggtgtcgcca tcgcgctcga
     7501 tcgcatggaa aaaggcacgg gtgagttgag cgcggttcag gaagtggaaa aacaatacgg
     7561 tctgcccgtc gcccccatcg ccagcctgaa cgatttgttt attctgttgc aaaacaaccc
     7621 cgaattcgga cagttcctcg aacccgtccg agcctaccgt cggcagtacg gcgtagaata
     7681 aaaacaaagc atatgccgtc cgaaccgcct tacgcctcag acggcatcaa acctgacaca
     7741 cacgaggaaa taccatgccc gcctgtttct gcccccactg caaaacccgt ctctgggtca
     7801 aagaaaccca actcaatgtc gcccaaggct tcgtcgtctg ccaaaaatgc gaaggactgt
     7861 ttaaagccaa agaccatctg gcaagcacga aagaacccat attcaacgat ttgcccgagg
     7921 ctgtttcgga tgtcaaactc gttcaccgta tcggcacgcg cgccatcggc aagaaacaga
     7981 tttcccgtga cgaaatcgcc ggcatcctca acggcggcac aacccagccc gatattccgc
     8041 ccgcaaccgc cgccacccct gctgccgcac cgcaggttgc cgtaccgccc gccgcgcccg
     8101 cccgtcagga tgggttcaac tggacgattg caaccctgtt tgcccttatc gtcctcatta
     8161 tgcagctttc ctacctcgtc atcctatgat cgtgcccgac ctctttgtcg cccacttccg
     8221 cgaagccgcc ccctacatcc gccaaatgcg cggcaaaacg ctggtcgccg gcatagacga
     8281 ccgcctgctc gaaggcgata ccttaaacaa gctcgccgcc gacatcgggc ttttgtcgca
     8341 actgggcatc aggctcgtcc tcatccacgg cgcgcgccac ttcctcgacc gccacgccgc
     8401 cgcgcaaggc cgcacgccgc attattgccg gggcttgcgc gttaccgacg aaacctcgct
     8461 cgaacaggcg cagcagtttg ccggcaccgt ccgcagccgt tttgaagccg cattgtgcgg
     8521 cagcgtttcc gggttcgcgc gcgcgccttc cgtcccgctc gtatcgggca acttcctgac
     8581 cgcccgtccg ataggtgtga ttgacggaac cgatatggaa tacgcgggcg ttatccgcaa
     8641 aaccgacacc gccgccctcc gtttccaact cgacgcgggc aatatcgtct ggctgccgcc
     8701 gctcggacat tcctacagcg gcaagacctt ctatctcgat atgcttcaaa ccgccgcctc
     8761 cgccgccgtc tcgcttcagg ccgaaaaact cgtttacctg accctttcag acggcatttc
     8821 ccgccccgac ggcacgctcg ccgtaaccct ctcggcacag gaagcgcaat cgctggcgga
     8881 acacgccggc ggcgaaacgc gacggctgat ttcgtccgcc gttgccgcgc tcgaaggcgg
     8941 cgtgcatcgc gtccaaatcc tcaacggtgc ctccgacggc agcctgctgc aagaactctt
     9001 cactcgcaac ggcatcggca cgtccattgc caaagaagcc ttcgtctcca tccggcaggc
     9061 gcacagcggc gacatcccgc acattgccgc cctcatccgc ccactggaag aacagggcat
     9121 cctgctgcac cgcagccgcg aatacctcga aaaccacatt tccgaatttt ccatcctcga
     9181 acacgacggc aacctgtacg gttgcgccgc cctgaaaacc tttgccgaag ccgattgcgg
     9241 cgaaatcgcc tgccttgccg tctcgccgca ggcacaggac ggcggctacg gcgaacgcct
     9301 gcttgcccac attatcgata aggcgcgcgg cataggcata agcaggctgt tcgccctctc
     9361 cacaaatacc ggcgaatggt ttgccgaacg cggctttcag acggcatcgg aagacgagtt
     9421 gcccgaaacg cggcgcaaag actaccgcag caacggacgg aactcgcata ttctggtacg
     9481 tcgcctgcac cgctgaccgc aacggaaagc cgccgcagaa atgccgtctg aaccccgttt
     9541 cagacggcat ttccccg
//
LOCUS       DAWHRS010000079         9394 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWHRS010000079 DAWHRS010000000
VERSION     DAWHRS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.079"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(61..1119)
                     /gene="dinB"
                     /locus_tag="WIB82_001320"
     CDS             complement(61..1119)
                     /gene="dinB"
                     /locus_tag="WIB82_001320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ3842614.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(1263..4352)
                     /locus_tag="WIB82_001321"
     CDS             complement(1263..4352)
                     /locus_tag="WIB82_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ3842615.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALLGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGMTVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILPCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEYAAGLSGEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDKCMSDPFALDFI
                     CQGSWGDPGVDADKAEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRYRVRPNT
                     GATHGVYAGSCQEEPDGDLSSPLVRGHKEPDWQAYDEKGNRTVYAEECRNAKKIKTEP
                     KLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGTPAFTGFSGTVPVWK
                     TVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYETSQTKDM
                     PVRYGQPASDFQTASSIRADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGSYTKIFK
                     QQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVDFGYVPG
                     AGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAAEVNAER
                     LAKNSSADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYG
                     LIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            4626..5405
                     /locus_tag="WIB82_001322"
     CDS             4626..5405
                     /locus_tag="WIB82_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ3842616.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGNTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(5616..7028)
                     /locus_tag="WIB82_001323"
     CDS             complement(5616..7028)
                     /locus_tag="WIB82_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ3842617.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKVKAELVGRYHSDCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            7119..7195
                     /locus_tag="WIB82_001324"
     tRNA            7119..7195
                     /locus_tag="WIB82_001324"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7153..7155,aa:Arg,seq:acg)
     gene            7213..7287
                     /locus_tag="WIB82_001325"
     tRNA            7213..7287
                     /locus_tag="WIB82_001325"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7246..7248,aa:Glu,seq:ttc)
     gene            7846..9021
                     /locus_tag="WIB82_001326"
     CDS             7846..9021
                     /locus_tag="WIB82_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ3842618.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
BASE COUNT         2370 a         2588 c         2126 g         2310 t
ORIGIN      
        1 gccatgccgt ctgaaaaccg ttcagacggc atattttttg gacggcgcgc gtaaagcggt
       61 ttacgcccac aaatcctgct gctggttttt cggcacaaga tgccccacgc cgataccgat
      121 aaggcggaac gcgtcttccg tctgcggcgg gacgcgcgcc atcaacattt gcgcagcctg
      181 cagcagagct gcgcagtcgg gcaatacgga ggaataagtc agtgtgcgcg tgatgatgcg
      241 gaaatcgtag gtcttcagct tgagcgttac gctttgggct tcgacgtttt tgcgcgtgat
      301 ttgccgccac aagtcttcgg caagatgggg gaggtgtccg gcagcctgct cgagcggcag
      361 gtcttcgggc agggtaattt ctgtggagat ttggaggcgt tcgcgttcgg ctttgacggg
      421 gcgttcgtcc gtaccgcgca ccaaatcata gaggcggtat ccgtagcgtc cgaaatggtt
      481 taagagttcg ccgcgctcga aacggcgcaa gtcgcccgcc gtccgcatac ccagcgactg
      541 catttttttc agcgttacct tgcccacgcc ggggattttg cccaaaggca gggtttccaa
      601 aaatgccatg actttgtgcg gcggcaacac aaactgcccg ttcggcttgc gccagtccga
      661 cgcgattttc gccagaaatt tgttcggcgc gatgcctgcg gatgcagtca aacctgtttc
      721 cgcaaaaatg gcggcacgga tttctttggc aacttcactg gcgtaaggga tgtttttgaa
      781 attacgggta acgtcaagat aggcttcgtc cagcgacaag ggttcgatta aatcggtata
      841 acgcctgaat acggcgtgaa tctgcgcgga aacctgacgg tacaaatcga aatgcggcgg
      901 cacatacacc gcttgcggac acagcctttt cgccgttgcc accgacatcg cggaatgcag
      961 cccgaactgc cgtgcctcat acgatgcggc gcaaatcacc gaacgcgcgc cctcccacgc
     1021 gacgaccacc ggccgccctt tcaaatgcgg ctgttcgcgc agctctaccg atgcgtagaa
     1081 tgcgtccatg tcgatgtgga taattttgcg tgaagacatc ggctcttctg aggataaaag
     1141 ggatattcta ctgccggcat cgggcaaatt ccaaatatac gccccgatag acctgcctcc
     1201 ataaaaatgc cgtctgaaac ataccctgtt tcagacggca tccgcaaaac tacggttttc
     1261 aattaaaact gccaatccag tttcatgctg acagtgcgcg gctctccgta gaagttgttt
     1321 gcgccgcgcg tacggttgta gttgttctca aaataagtgc gtccgtttaa gttcgtaccg
     1381 atgaggctca atttggcgtg tttgcccaat tcgtaacgga cgaaaccgtc tatcagcccg
     1441 tagccgccct gcctgatgtt atacagactg cttgtgccgc tttgtgcgga cacgccgccg
     1501 ccgacggtca gccccgtatt cggtatatgg aagctcgttc cgaaacggaa tatgtgcacg
     1561 ggtgtgaaat tgctgaagtt gtacgggtct gcactggaat ttttggcaag gcgttcggcg
     1621 ttgacttcgg cggcgttttt gtagcggctc ttgttgtagg tgtaacccgc aaagactttc
     1681 caatcttcgt tcaactcacc cgacaactcg aattccgcac ccctgctgac cactttgcct
     1741 atcggtttgg caacggtttg gaacgacccc tgcttgccgc ctgctccggg aacatagccg
     1801 aaatcgacga ccgtgcggtt tttctgttcg aggtaaaaca atgcgaacga agcattcagc
     1861 cgtccttgca agaacgcgcc tttccagcct acctcatagt ttgtgccgac caaaggcggt
     1921 aaaacggttt tggcactgac atcgacatta tcctgctgtt tgaagatttt ggtataactt
     1981 ccgtaaatac tctgttgcgg tgtcaagtca taggtaatgc ctgcataggg cgtcaattta
     2041 tgaccttgca tcttggccgt gtaatggtcc tgatccgccc taatgctcga tgccgtctga
     2101 aaatcgcttg ccggctgccc atagcggaca ggcatatctt tggtttgcga agtctcatag
     2161 cgcgtgtagt gcagcccgcc caaaaggtgc agtcggccgg ttacgttgaa acgcgtgctg
     2221 gcagtcagcg aatgggtttt gttggtgttg aggtatttgg cgtagttata cagcgcagga
     2281 acatggtcgt ctgccacttt gacggttttc caaaccggca ccgtaccgga aaaaccggta
     2341 aaggcaggcg tgccgtcggg attggtctcc tgaatcttgt tgcctttttc gtccagctca
     2401 tatacatcga catataccgg tgtccggctg ccgctgtatt cgtcatagta atacacctgc
     2461 ttgccttcgg catcgagctt gggctcggtt tttattttct tggcgttcct gcattcttcg
     2521 gcataaacgg tacggttgcc tttttcatcg tacgcctgcc aatcgggttc tttatgcccc
     2581 ctgaccaaag gagacgacaa atcgccgtcc ggctcctcct gacaacttcc cgcatacacg
     2641 ccgtgcgttg cccccgtatt cggacgtact ctgtagcggc gttcgtagat ttctagatat
     2701 tccgaacgta tcttttcatc accgtaggca tagccgacaa agaaatcatg ctcccgcccg
     2761 aacagcccat atgtgccggt caggtcaagt ttaattcccc attggcggtc gtctttggta
     2821 tgccgcaacg gcatataact gtatcgtcgg ttggcggtag ccttccgatt aaaggaagag
     2881 tcatacaggc tgtttggaaa acgttgtgcc gcattattaa agatgccctc cttcgcaagg
     2941 gctttatcga caaattccgc cttgtcggca tcaacgcccg gatcccccca agaaccttga
     3001 cagataaagt ccagcgcgaa agggtcactc atacacttgt caaaaccggc tttgcgttct
     3061 gcggcacggc ggctgcgata ctgttcgaaa gcagtattat cgaaacggtt tttaacaaaa
     3121 tcgtctttgc gctcccggta ttccttggcg gtttcatcac gatatgcttt cagtttctcc
     3181 aatgccttat ctttcggctc gaacgggatg acttcgtttt tttcagtcaa aaagcctacc
     3241 gcatcctcac ccgacaaacc cgccgcatat tcgtttttca gaaaaaactg ccccaccttc
     3301 gcatcggatt cattcttggt ataagacact tcggcattga gctgccaacc gttgtcaaac
     3361 acatgtttga atcctgagaa aaggttgtat ttgtcggcac ttaaccgcga ccaatcctcc
     3421 cccaaataag tgttgcgcgg cagttgcaaa ggccggttgc aggcaggcgt tgaactgaac
     3481 ggggcagttt tctgattttc acagggcaaa ataatgcccg aaaaatcagg aacctcccta
     3541 ctcttctgat acatgccgcc caaagtaagc acactgctgt cgcccgcatc ggcttcggca
     3601 atgccgtaaa ccatatgttt cctgccccaa acgcggtctt taaacgattt tttatactct
     3661 tccgcaccca ccaaccttcc gcgtaaggta ttcgccttat tcaggctgcc tgaaacatcc
     3721 aacactgcac gccggctgcc gcgatggtcg gcggtcagct ctccggtatg tttgaaagaa
     3781 gcggtaggtc gcttacggat caaattgacg gttcctcccg gctctgaatt ggattgggtc
     3841 aaccccgttg caccccgtac aacttcaata tggtcataaa ccgccaaatc ggtactcgga
     3901 gacacgtcga ttttcgccgt atatcccgaa cggcctgcaa cattgacggt cataccgtct
     3961 tcaccaatct gatcaatata gaaaccgcgt gacaaaaacc gcgtctgcaa gcctgaatcg
     4021 cgcacaacgt tgacacccgt cgtgtttttc attgcctctt caagcgtatg caccgcctta
     4081 tcgtcaaggc ggctgcgcgt gatgacgctg accgactgcg gcgtatcctt gcccgcaatc
     4141 ctcatacctg tggcggtgga catccgatct atcgtataag aacgggtctt ttcggtcttg
     4201 cccaacaaag catgagagcc gcgtacattg accgtatcca gactgacggt attgccgtct
     4261 gaaacaggca caacaccgtc tgcaaaagaa ccaccgtaag ccgataacag cataacggtc
     4321 agaattttaa gtgaaaaatg attttgattc atagagacct ctgtaatatg caagtgtgca
     4381 aatcgtccaa aggctctcac aactgttttg attttttata ttaattgaaa aaaagtaatt
     4441 ctcaattaaa tttatagata ggattgtatt cccattttga caaaaaacaa actactcctt
     4501 accgtttatt tcaaaaaacg ataacattgt attgaaaaat atccgaattt aaatacagac
     4561 cgccaatgca gaaaaaaaca cccaaattgg ctataatccc gacaaacaca ctcaaggaca
     4621 acaacatggc agcctcgccc gaagcaaaat tcaccgaaga aaagattttg tgggtcaaac
     4681 accacacgcc gaaactcatc actttcgcca tcagccgtcc cgaatcctac cgctttaaag
     4741 ccggacagtt ctcccgactc ggtttctacg aaggggaagg tttcatttgg cgtgcctatt
     4801 ccattgtttc cgcagaatat gccgacacgc tcgaatattt tgccgtactc atccaagacg
     4861 gccccatgtc ggcccgtttc gccaaaatgc aacagggcaa caccatcctg ctcgataaaa
     4921 atgccaccgg cttcctcctg cccgaacgct tccccgacgg caaggatttg gtgatgctct
     4981 gcaccggctc gggcatcgcc cccttccttt ccattctcga acaacccgaa atccgtcaac
     5041 gtttcgatac cgtcaacctg atacattccg tatcttttcc cgaagaattg attttcaacg
     5101 accgactcgc cgcattgact gaacatcccc tggtaggcga atacggacac tctttccgtt
     5161 tcgtccctgt taccacccgt gccgccaacc cctcgggctt aagcggaaaa cgcattccgg
     5221 aactcttaaa aaacaacagc atcgaacagg cgctgcatac caagttcacc ccggaatcca
     5281 cacggtttat gatttgcggc aacccggaaa tggtcaaaga cactttccaa acgctgctcg
     5341 acatgggtta cgccatgcac cgcaaccgca ttcccggtca aatcatgatg gaaaacggct
     5401 tctaaaaacc accctgcttg tccgatgcct tcggatggac gggcaaaccg acacggcacg
     5461 aaaaccgcgt cggcaaaaat gccgtctgaa aaaattcaga cggcatcttc ggatacatta
     5521 cctgcaaacg gcaacacacc ggcacaaacc gattaggcaa tcaacacggt gacggctgtt
     5581 tacatacttg ccggctttca ccaaccgata tcgatttaac cgatttcctt aatatttttc
     5641 ctgtccgttt taaacttcgc cttaaacgca tccggtaaat ctttatcgaa ataccaaagc
     5701 ccgtcatcca tttccaatgc gccgcccatt ccgtgcagaa cgactttttc ccccgtcaaa
     5761 ggatcggttt cggtaatttc cacctcatgc atttttcccc aatccaaaac aggcaacttg
     5821 tgtgcaaacg ccaaaacctg ttcgtcagaa cggccgcacg ccttatcgca tcggcctgaa
     5881 acatatacgg gacaaggctc atcctctgca taaccgtccg gaaggatacc ggctgcggca
     5941 ggacacaatc cgtatgtttc cgcccacgac gacaaagccc gtctcgctgc ctttttattg
     6001 gcaaataatc cggtaggcgg attatccgtc acaccgtttt tcaaagccgc tgttttcgca
     6061 ttcaacatgc cgtctgaacc tttttcaaac ctgacggtcg taaatgtttt aagcagattt
     6121 ttggcagaca cataacaatc cgaatgataa cgcccgacca attccgcttt aaccttatat
     6181 gcatgcaggc tgcccaatgc gggaaaaaaa cggacttcct ccgtattgca ccaatcaaac
     6241 ggggcttttc cggagtccaa taaagccgaa acctcgctat atacccgttc aaacgtaccg
     6301 atataattta ctttccctcc gccgtcgaaa caagccagca cccccatacc gtcaggcaaa
     6361 ccgtacaact gttccctcaa ccgttcgggc agcgcggcag gcagcggttt cggattcatc
     6421 aaacggaaac actgcctgat ccatgcctca accccgtgtt ccgacagact gtattccaaa
     6481 taatcacaca atgccgatac atccgccatc gcacgatgcc tgtcttccac aacaatcccc
     6541 aacctttcga tgatactgtc caggctgtgc ttgtaaaatt gcggatacag acaccgggac
     6601 agctgcacac tgcacaaagc aggcgatgaa aatccgatac ccgcacgatg aaactcatgc
     6661 tttaaaaacg tatagtcgaa acggctgtta tgtgcaacca gcacacaacc cttcaatacc
     6721 gaaaacaact cgccggcaat ctctgcaaaa acaggcgcat cggcaaccat gccgtctgaa
     6781 atccccgtca gccccgccac aaactgcgga atcggttttt gaggattaac caaccactca
     6841 tgcctcacca cccttccctg ctcaaacttg accaaagcca cttcggttac cctgtcttca
     6901 tacagattgc cgcccgtcga ttccaaatcg accacggcaa caggcattcc aaaccgtaaa
     6961 aatacctttt ccagcaaggg ccagcgggaa gcaacaatca ttttattctc tttaaattca
     7021 aacaacaaac cgatatttta cacttttaag gcatttcatc caacaaaaca attgacagaa
     7081 tccgatgatt accctaaaat tcaaatcttt cttgcagtgc acccgtagct cagttggata
     7141 gagtatctgg ctacgaacca gagggtcggg cgttcgaatc gctccgggtg cgccagtaag
     7201 aaaatacaat atgcgcccat cgtctagcgg ttaggacatc gccctttcac ggcggtaacc
     7261 ggggttcgat tccccgtggg cgtgccaaat tccaaatccc ccgaaataat ccctcgggga
     7321 tttttattgc ctcggcaatt cattaccata tctttaccta cccccttcat cagactctca
     7381 gacgtaatcg aatcatattc aaacctttgc cgtgcaaacc gatatcacgt aaccggatgc
     7441 ggtgtccgtc caacatttta cccgattgaa acgcctgata tattgcaccc catcaacgtg
     7501 gcattacttt tcttaacaat cccctttgac agcaactgac tagggctttt ttatgccatc
     7561 atcaaattta tagtggatta actttaaacc agtacggcgt tgcctcgcct ttccgtacta
     7621 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaattcac tataatattt
     7681 tctctcccga ttgaaacagg cgtaacagaa tatgcccgaa gctccggctg ctttcttgtt
     7741 taccgccgcg atatttagag tataatacca aatttgagca atagttctaa aacagttaga
     7801 accatttttc atgagcctga ctgattcgta tactcggaga aactgatgca aaatattttt
     7861 gaccctttgg ttattcgtgg aaaatccctt acccccatcg tgcaaggcgg tatgggggtc
     7921 ggtgtttccg catcgggttt atccagcgcg gtggcacgtg aaaacggtat cggaacgatt
     7981 gccagtgtgg atttgcgcca ccttcacgaa gacctactcg ccgaatcaca aatcaatccg
     8041 agtgaagaga aatatacatc tttgaactgt accgcattag acagggaaat ccaaaaagcc
     8101 aaaagcgctt cagagggaaa aggactgatt gcggtcaacg tgatgaaggc ggtcaaagac
     8161 cacgccgcgt atgtccgcca ggcttgcgaa tcaggggcgg atgcggttgt aatgggtgcc
     8221 ggcctgcctt tagacctgcc ggaaatgacc gagggctatc ataaagatgt cgcgctgctg
     8281 ccgattctgt ccgaatcgcg cggtattaat atcgtcttga aacgttggat gaaaaaaggc
     8341 atattgcccg atgcgattgt agtcgaacat cctgcccacg cggccgggca tttgggcgcg
     8401 tcaaccgtcg aaggcgtaaa cgatgctaaa ttcgacttca aacgcgtgat tgaggaaacg
     8461 tttgaagttt tcaaaagttt agggctggaa agcgaaaaaa tcccgcttat tcttgcggga
     8521 ggcatggcaa attttgaaaa agtcaaaacc gccctaaaga actggggagc atccgccgtt
     8581 caaatcggta cggcttttgc cgttaccgaa gaaggagatg cacaccttaa cttcaaaaaa
     8641 acgctcgccg gtgcggaaac tgaaaaagta gtcgaattta tgtctgttgc cggtttgccg
     8701 gcgcgcggtg tccgcaccaa attcctagac agctacatca agcgtgaaag caaacttcag
     8761 acaaacgcca aagccgaccc gcgccgctgt acccaaggtt taaactgcct aaccagttgc
     8821 ggtctgcgcg acgggctttc caaagccgga caattctgca ttgatatcca gcttgccgcc
     8881 gcattccgtg gagaagtaga taaaggcctg ttcttcagag gtaaagaccc gctgcccttc
     8941 ggcaatgcca tccgcaccgt ccgcgagacg atacaatatc tgctgacggg gagcgaacct
     9001 gttgcaacgc tcggacgctg acatcaggta ggatttgatt tgcaaacaaa atgccgtctg
     9061 aagcctttca gacggcattt ttcaggctgc gtttcggaat agtgtcgaaa aaacggagag
     9121 aaataaaaac gtttttatgt aaacaaaacg ccttccgcat tgtgtcgggc atacaacaca
     9181 aatgccgtct gaagcccttc agacggcatt tttcaggctg catttcggaa tggtgttaaa
     9241 aaataaacgg gataagatac atttattttc gtccgataaa tcagaccatc acgccgaata
     9301 atcaaacaat gcctgcccga cattgcatct ggcaaaccaa tgcaatgaaa acacggcttt
     9361 tttatttgct tccggcatta ttgaaaagtt tatc
//
LOCUS       DAWHRS010000080         9286 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWHRS010000080 DAWHRS010000000
VERSION     DAWHRS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.080"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            46..1722
                     /gene="pilB"
                     /locus_tag="WIB82_001327"
     CDS             46..1722
                     /gene="pilB"
                     /locus_tag="WIB82_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ3842619.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(1807..2016)
                     /locus_tag="WIB82_001328"
     CDS             complement(1807..2016)
                     /locus_tag="WIB82_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ3842620.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(2009..2641)
                     /locus_tag="WIB82_001329"
     CDS             complement(2009..2641)
                     /locus_tag="WIB82_001329"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ3842621.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            complement(2643..3503)
                     /locus_tag="WIB82_001330"
     CDS             complement(2643..3503)
                     /locus_tag="WIB82_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ3842622.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKKHLNLPLTEEESRTFNLMKPDSCCPKCRVPIRARQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            complement(3577..4809)
                     /gene="pilG"
                     /locus_tag="WIB82_001331"
     CDS             complement(3577..4809)
                     /gene="pilG"
                     /locus_tag="WIB82_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ3842623.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            5319..6962
                     /gene="pgi"
                     /locus_tag="WIB82_001332"
     CDS             5319..6962
                     /gene="pgi"
                     /locus_tag="WIB82_001332"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ3842624.1"
                     /translation="MDAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCNLADAADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDEVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETARHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGVGAA"
     gene            6932..7123
                     /locus_tag="WIB82_001333"
     CDS             6932..7123
                     /locus_tag="WIB82_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842625.1"
                     /translation="MPSERRRRGIKAMPAARPIPKRQCSATARVLLTNMRLRGTIAHS
                     FQMNILPENTGKRPTKHLT"
     gene            7133..7954
                     /gene="dapD"
                     /locus_tag="WIB82_001334"
     CDS             7133..7954
                     /gene="dapD"
                     /locus_tag="WIB82_001334"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ3842626.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            8109..8894
                     /gene="fabI"
                     /locus_tag="WIB82_001335"
     CDS             8109..8894
                     /gene="fabI"
                     /locus_tag="WIB82_001335"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ3842627.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGADLAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(9138..>9286)
                     /locus_tag="WIB82_001336"
     CDS             complement(9138..>9286)
                     /locus_tag="WIB82_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HEZ3842628.1"
                     /translation="ISPVTSFVIDGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWL
                     VEVC"
BASE COUNT         2127 a         2447 c         2597 g         2115 t
ORIGIN      
        1 aagctgtaca ggtataacaa tgaataaatt tggggataag gtcgtatgag cgtaggtttg
       61 ctgaggattc tggttcaaaa ccaggtggtt actgttgagc aggccgagca ttactacaat
      121 gagtcgcagg cgggtaagga agtgttgccg atgctgtttt cagacggtgt catttcgccc
      181 aagtcgcttg cggcattgat tgcgagggtg ttcagttatt cgattcttga tttgcgtcat
      241 tatccgcgcc acagggtgct gatgggggtg ttgacggagg agcagatggt ggagttccac
      301 tgtgtgccgg ttttccgtcg gggcgacaaa gtattttttg cggtttccga tccgacacag
      361 atgccgcaaa ttcagaaaac cgtttctgcc gcagggattg aggttgaatt ggtcattgtc
      421 gaggatgacc agttggcggg tttgctcgat tgggtgggtt cgcgttcgac atcgctgctt
      481 caggagcttg gggaggggca ggaggaagag gaaagccaca ccctgtatat cgacaacgag
      541 gaggcagaag acggccctgt tccgaggttt atccataaga ctttgtcgga tgccttgcgc
      601 agcggggcat ccgacatcca tttcgagttt tacgaacaca atgcccgtat ccgtttccgt
      661 gtggacgggc agctccgcga ggtggttcag ccgcccattg cggtaagggg gcagcttgct
      721 tcccggatta aggtaatgtc gcgtttggac atttccgaaa aacggatacc gcaggacggc
      781 aggatgcagc tgacctttca aaagggcggc aagcctgtcg atttccgtgt cagcacattg
      841 ccgacgctgt ttggcgaaaa ggtcgtgatg cggattttga attccgatgc cgcgtctttg
      901 aacattgacc agctcggttt tgagccgttt cagaaaaaat tgttgttgga agcgattcac
      961 cgtccctacg ggatggtgct ggtaaccggt ccgacgggtt cgggtaagac ggtgtcgctc
     1021 tatacctgtt tgaatatttt gaatacggag tcggtaaata ttgcaacggc ggaagaccct
     1081 gccgagatta acctgccggg catcaatcag gttaacgtca atgataagca gggtctgact
     1141 tttgccgctg ctttgaagtc tttcctgcgt caggacccgg acatcattat ggtcggtgag
     1201 attcgtgatt tggaaactgc cgatattgcg attaaggcgg cacaaacagg gcatatggtg
     1261 ttttccaccc tgcacaccaa taatgcgccg gcgacgttgt cgcgtatgct gaatatgggt
     1321 gtcgcgccgt ttaatattgc cagttcggtc agcctgatta tggcgcagcg tcttttacgc
     1381 aggctgtgtt cgagctgcaa acaggaagtg gaacgcccgt ctgcctctgc tttgaaggaa
     1441 gtcggcttca ccgatgagga ccttgcaaaa gattggaaac tttaccgcgc cgtcggttgc
     1501 gaccgttgcc gggggcaggg ttataagggg cgtgcgggcg tgtatgaggt tatgcccatc
     1561 agcgaagaaa tgcagcgtgt gattatgaac aacggtacgg aagtggatat tttggacgtt
     1621 gcctataagg agggtatggt ggatttgcgc cgggccggta ttttgaaagt tatgcagggc
     1681 attacttcat tggaagaggt aacggcaaat accaacgatt aggtttgaga atgaaaatgc
     1741 cgtctgaagc gtgtttgttt cagacggcat ttgactttca gggtgtttgc cgggaaggcg
     1801 gggcggtcag cggtatgcca tgtcgggttc ggatatttcc ggcaaacttt ccgtttggcc
     1861 ggaaaccgta tatttcccgt ctgcccatcc gcccaagtcg atcagtttgc agcgttgcga
     1921 acagaagggg cggaatgcgt tttcgggttc ccatactact gctgtttgac aggtcggaca
     1981 tttgacttga aggcgtgttt gccgcgattc agccattgtg ttttccttgt gttggttttg
     2041 aggcgaaaat ccctgaataa aacgcgtgca ggcgcattgt tttctcacgc aggcttttga
     2101 ggctgccgtc attgagcagc acatcgtctg caagcagcag gcgttcggat tcggatgcct
     2161 gatggctgat gacggcagcc acctcgccgc gcgtcagccc gctgcgggcc atcaccctgc
     2221 cgatacgttt ttccaaaggt gcgcttatgg tcaggacacg ccgtatcagg ctgataaatt
     2281 gacgcttttc cgtcagcagc ggaatttcga caatgccgta agccgcatcg gtaaaggttt
     2341 cttgctgttt tttgatttct gagaaaatca gcggcaacat cacggattcg agcaaggctt
     2401 ttcgcgatgg ggaggcaaag acttctttac gcaatatgtc gcgccgcaac aaaccctgtg
     2461 tgtcgaaaac ggtgtcgccg aacagccgcc tgatttccgg cagggcgatg ccgtctgaag
     2521 ccgtcagcga gtgcgccgcc gcgtccgcat cgatgcgcgg cacgcccaaa tcggcaaaac
     2581 attgcgcggc tgccgatttg ccgctgccga ttccgccggt cagtccgacc cataccgtca
     2641 tcttacagca ccggatgggt cagccaccag ttgaccgccc gccatacgga atcgtttgcc
     2701 gtaaaaatta tccagcccga aactgtcagt gcggggccga aggcaaaatg ctgccccttg
     2761 gcgacgcgca taacgattgc cgcgaccaaa ccgatcagcg aggaaacaaa aatcagcacg
     2821 ggcaatgcgg atatgccgag ccacgcgccc aatgcggcaa tcagtttgaa atctccgttg
     2881 cccataccga tttcgccttt aatcagttta tataccgcgc ataaaagcca tatcgaacca
     2941 tagccggcaa ccgcacctaa aacggcagac tgcaaaggca cgaagccgcc gtccaaatta
     3001 aatatcagcc ccagccaaat caagggtaat gtcatcgagt cgggcaggta ttgggtgtcc
     3061 gcatcgataa aggtcaggga aatcagaaac gcggtcagta tcaaaccgcc caatgtaatc
     3121 caagaccagc cgtattgcca ggcaaccagc ccgaacaata cgccggtcag cagttcgatt
     3181 aagggatagc gtatgctgat tttggtttgg caggaggcgc atttgccgcg caggagcagg
     3241 tagctgacaa tcgggatgtt ctgccgcgcg cgtatcggca cgcgacattt gggacagcag
     3301 gaatccggtt tcatcaggtt gaaggtacgg ctttcctctt cggtcagcgg caggtttaaa
     3361 tgttttttgg caaataccgt ccagccgcgt tccatcatga ccggcacgcg gtaaatgacg
     3421 acgtttaaga aactgccgac cagcagcccg aacaccgctg ccaaaggcac ggcaaacggc
     3481 gacaatacag acaaatcaga catattttgt tctcaatgta ttcaaaacaa aaacaaaccg
     3541 gcgcagagcg aatccgcgcc ggatctgtgc ggcaaatcag gcgaccacgt tgcccaaatt
     3601 aaacagcggc agatacatgg cgaccagaag cgtgccgatg accaagccta aaatcacgat
     3661 aatgatcggc tccatcatag cggacagcct gccgaccgca ttgtccacct cgtcttcgta
     3721 aaattcggcg gctttgttga gcatatcgtc caaagaaccc gattcctcgc cgatggaaga
     3781 catctgcaac atcatattgg ggaacagttc cgtcgcacgc atccccgaag tcatagacaa
     3841 accttggatg acgcgcgtac ggatttcccg ggtggcttct tcatagatta aattgcccgc
     3901 cgcgccggca gtggagtcca atacatcgac caaaggcacg cctgccgcaa tcagcgtcgc
     3961 tgtcgtcctg ccccagcggg caatcgtccc tttgcggaca atgtctccga aaatcggcat
     4021 acgcagcagt atggcatcca tacgccgttg gattttaatc gaacgcgcct tcaatttaag
     4081 gaagccgtat atggcaaagc ccagtgcgat cagcaccatc cagccgtatg agacgaaaaa
     4141 gtcggacata tccatcactg tttgggtcag tgcgggaagc tccgcgccca tattggcgta
     4201 aacttcttta aaggcgggca gtacgaaaat catcatcacg aataccaaac cgatggcgac
     4261 ggcgatgacg gataccggat aggtcagcgc ggtttttacc tttttgcgga tggcctgggt
     4321 tttttctttg taaattgcca atttgtccag caggctttcc aatacgccgc ccgtttcgcc
     4381 cgccgcaacc aggttgcagt agaagcggtc gaaatatttc gggtggtttg agaatgcgcg
     4441 gctcaacgag ctgccctgtt ccacttcgcc tcggatttcc atcagcattt ccgtcataga
     4501 cgggttgccg tgtccgcgcg ccacgatttc aaatgcctgc atcagcggca ggcccgcttt
     4561 aatcatcgtg gacagctggc gggtgaaaac ggtgatgtct tcttgtgtga ttttgcgctt
     4621 ggagcttgtt ttcacacggg taatctgcaa cgggcggatg ccgcgttttg ccagtttttt
     4681 gcgcgcctct tcttcggtaa acgcggatac ttcgccgttg accagtttgt cggaggcgga
     4741 atgcctgcct tcaaagataa agcgtttttc tttctttgcg aacaaagaaa atcctccgtt
     4801 tttagccata ttctagcccc gtaaagtaat tggaataaaa tgtaagaaac atcgttaaaa
     4861 aacagtaccg gcgtgttccc ggtaagatga aaaccgccga catcccgcct gcgggcggca
     4921 aacgggacag aatcggatgc gattatacct tatttaggcg gctgtccggc atttatgcgt
     4981 acacaataaa tcttgcagga tattgttgcg ggtcaaatgc cggccggagg gtatagtgaa
     5041 ttaacaaaaa ccggtacagc gttggctcgc cttagctcaa gagagaacga ttctctaagg
     5101 tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtttgcggc tcgccgcctt
     5161 gtcctgattt ttgttaattc actatatttt cgccatatgg aaataaggtg ctattggacg
     5221 cggcgggcgg tgttccggag attcgccaaa gccgctgccg tttgttaaac tacattctgc
     5281 tacattttaa tccggttctg aaaaatcaag gaaaacagat ggatgctttt acccgtgcat
     5341 ggtatgcgct cgaacgccat tatcaggata cgcgtcatgt ccttttgcgc gaccgctttg
     5401 cctgcgaacc cgaccgtttt gagcgtatgc acgagcgttt ggacgggatg ttgttcgatt
     5461 acagcaaaaa ccgtttgggc gaagatacgc tgcaactgct ctgcaatctt gccgatgcgg
     5521 cggatttgga agggaaaatg cgtgctttgc ggacgggtgc gaaagtcaac ggcagcgagg
     5581 ggcgtgccgc gctgcatacg gctttgcgcc tgcccgacgg tgcgggtgcc gtttatgcgg
     5641 acggcaggga cgtgttgccc gaaatccgcc gcgagttaaa tcgtgcgttg aagtttgcac
     5701 acagtttgga cgacggttcg tatcagggga caaccggaaa acggattacg gattttgtcc
     5761 acatcggcat aggcggatcc gacctcgggc cggcaatgtg cgtgcaggca ctcgagccgt
     5821 tcagacggca tatcgccgtc cattttgccg ccaacgccga tcctgcctgc ctggatgagg
     5881 tcttatgccg tctgaacccc gaaacgacgg tgttttgcgt tgccagcaag tccttcaaaa
     5941 cgcctgaaac cctgctcaat gcacaggcgg tcaaggcttg gtatcgtggt gcggggttct
     6001 cggaatccga aacagcacgc catttttgcg cggtatcggc cgacacagcg gcagctgcgg
     6061 ctttcggtat cgcggcggaa cgcgtgtttg cgatgtacga ctgggtgggc ggacgctatt
     6121 ccgtctggtc gcccgtcggt ttgcccgtga tggttgcggt cggcggggcg cgtttccgcg
     6181 agttgttggc gggggcgcac gcgatggaca gccatttttt ccacacgccg ccgcgccgca
     6241 acattcccgt tctgatggca ctgattgccg tgtggtataa caatttccaa cacgcggacg
     6301 ggcagaccgc cgttccgtac agccacaacc tgcgcctgct gccggcgtgg ctgaaccagc
     6361 tcgatatgga gagtttgggc aaaagccgcg cttcagacgg cagtcccgcc gtgtgcaaaa
     6421 cgggcggcat cgtgttcggc ggtgaagggg tcaactgcca gcacgcctat ttccaactgc
     6481 ttcatcaagg cacgcgcctg attccctgcg actttatcgt cccgatgacg gcgcagggcg
     6541 tggaggacgg acgcagccgt tttaccgttg ccaacgcctt tgcccaagcg gaagccctga
     6601 tgaagggcaa aaccttggac gaagcacgcg ccgaactggc agatttgccc gaagcggaac
     6661 gcgaacgcct cgcgccgcac aaagaattcc ccggcaaccg ccccagcaac agcatcctgc
     6721 tcgaccgcct cacgccctac aatctgggta tgctgatggc ggcttacgaa cacaaaacct
     6781 tcgtccaagg cgcgatatgg aacgtcaacc ccttcgatca gtggggggtg gaatacggca
     6841 aacagttggc gaaaaccatc atcggcgaac tggaaggcgg cacgtccgta cacgatgcct
     6901 cgaccgaagg gctgatggcg ttttaccgtg aatgccgtct gaaaggcgtc ggcgcggcat
     6961 aaaggcaatg cctgccgccc gcccgattcc gaaacgccaa tgttcggcaa ccgcccgcgt
     7021 attgctgacg aatatgcgtt tgcgtggcac aatagcgcat tcatttcaaa tgaacatact
     7081 gcctgaaaat accggcaagc gtcccacgaa acatctcaca taaggaaaca ttatgtcttt
     7141 gcaaaacatt atcgaaaccg cctttgaaaa ccgcgcggac atcaccccga ccaccgttgc
     7201 cccagaagtt aaagaagccg tgttggaaac catccgccag ctcgattcag gcaaactgcg
     7261 cgttgccgaa cgcttgggcg tgggcgaatg gaaagtcaac gaatgggcga aaaaagccgt
     7321 gttgctgtcc ttccgcatcc aagacaacga agtcctcaac gacggcgtga acaaatactt
     7381 cgacaaagtg ccgaccaagt ttgccgattg gtctgaagac gagttcaaaa acgcaggctt
     7441 ccgcgcagtt ccgggtgcgg ttgcccgacg cggcagcttt gtggcgaaaa atgtcgtgct
     7501 gatgccgtct tacgtcaaca tcggtgcata cgttgacgaa ggtgcgatgg tcgatacttg
     7561 ggcgaccgtc ggctcttgcg cgcaaatcgg taaaaacgtc cacttgagcg gcggtgtcgg
     7621 catcggcggc gtactcgaac ccctgcaggc cagcccgacc attattgaag acaactgctt
     7681 catcggtgcg cgttctgaaa tcgttgaggg cgtgattgtc gaagaaggca gcgtgatttc
     7741 tatgggcgtg ttcatcggtc aatccaccaa aatctttgac cgtacaaccg gcgaaatcta
     7801 ccaaggccgc gtaccggcag gttcggttgt cgtatccggc agtatgcctt ccaaagacgg
     7861 cagccacagc ctttactgcg ccgtcatcgt caaacgcgtg gacgcgcaaa cccgtgcgaa
     7921 aaccagcgtc aacgaattgt tgcgcggcat ctgatgcctt aaaccgtatt tgaaacgtcc
     7981 gatgccgtct gaaatccgct tcagacggca ttgccgtttg cacgctgcaa cgtgaaaaca
     8041 cagaaacagg gacaatttgc tataatcaac ggtttagaac gaaccgaaca ctatttgaag
     8101 gatacaaaat gggttttctg caaggcaaaa aaattctgat taccggcatg atttccgagc
     8161 gttccatcgc ttacggcatc gccaaagcct gccgcgaaca aggcgcggat ttggcgttta
     8221 cctacgttgt ggacaaactg gaagagcgcg tccgcaaaat ggcggcggaa ttggattccg
     8281 aactcgtatt ccgctgcgat gtcgccagtg acgacgaaat caaccaagtg ttcgccgact
     8341 tgggcaaaca ttgggacggc ttggacggtt tggtgcattc catcggcttc gcaccgaaag
     8401 aagccttgag cggcgatttc ctcgacagca tcagccgcga agcgttcaac accgcacacg
     8461 aaatttccgc atacagcctg cccgcattgg caaaagccgc ccgtccgatg atgcgcggca
     8521 gaaactccgc catcgttgcc ctgagctact tgggcgcggt gcgcgcgatt ccgaactaca
     8581 acgtgatggg tatggcaaaa gccagccttg aggcgggcat tcgctttacc gccgcctgtt
     8641 tgggcaaaga aggcatccgc tgcaacggca tttccgccgg tccgatcaaa acactcgccg
     8701 cctccggcat cgccgatttc ggcaaactct tgggacacgt cgccgcccac aatccgctgc
     8761 gccgcaacgt taccatcgaa gaagtcggca ataccgccgc cttcctgctg tccgacctgt
     8821 cgtccggcat taccggcgaa atcacttacg ttgacggcgg ttacagcatc aatgccttga
     8881 gcaccgaggg ataatccgcc gttttcaaat ccgtgcgccg tccgtgccgc atatcggttt
     8941 cgggcggcgt tttgccgtct gaagcgtatt tctagggaaa tgcccgactt acggcaggcg
     9001 ggatgggaaa tgcggacgct tgttttaacc gattgccttt gtgccgactt gctgcaggtg
     9061 cagcggaaac ggttcggatg cgaaaatgcc gtctgaaacg ccaaacgggt ttcagacggc
     9121 attttttatt taaagcatca gcacacttca accagccagc cgtatttgtc ttccgccaaa
     9181 ccatactgga tgtcggtaat cgccttacgg atggcgtagc cgcgttcttg gcttttcact
     9241 tcgatttctt tgccgtcaat cacgaaagaa gttaccggag aaatga
//
LOCUS       DAWHRS010000081         9111 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWHRS010000081 DAWHRS010000000
VERSION     DAWHRS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.081"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(33..758)
                     /locus_tag="WIB82_001337"
     CDS             complement(33..758)
                     /locus_tag="WIB82_001337"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ3842629.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDTGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNTVPLKEEIGRREVACKGL"
     gene            complement(828..1262)
                     /gene="fur"
                     /locus_tag="WIB82_001338"
     CDS             complement(828..1262)
                     /gene="fur"
                     /locus_tag="WIB82_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ3842630.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1481..1858
                     /locus_tag="WIB82_001339"
     CDS             1481..1858
                     /locus_tag="WIB82_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ3842631.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1868..2677
                     /gene="dapB"
                     /locus_tag="WIB82_001340"
     CDS             1868..2677
                     /gene="dapB"
                     /locus_tag="WIB82_001340"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ3842632.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDTPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2791..3201)
                     /locus_tag="WIB82_001341"
     CDS             complement(2791..3201)
                     /locus_tag="WIB82_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842633.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFIYGYATKSQDKDIDNILELGELKQDDEILDYGGT
                     LELIGGRCDLLTGFSIDIVCREIELEFLDQKSFN"
     gene            complement(3203..3448)
                     /locus_tag="WIB82_001342"
     CDS             complement(3203..3448)
                     /locus_tag="WIB82_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842634.1"
                     /translation="MDYLLEAARITKEGGEIVINGTSNNKYLRGIPNEGELARVGLRL
                     KYNGPLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(3456..4628)
                     /gene="lpxB"
                     /locus_tag="WIB82_001343"
     CDS             complement(3456..4628)
                     /gene="lpxB"
                     /locus_tag="WIB82_001343"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ3842635.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFAEVVKRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAERLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATAATKRRLAEILQRSEFAGLPLTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRVLHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4676..5668)
                     /locus_tag="WIB82_001344"
     CDS             complement(4676..5668)
                     /locus_tag="WIB82_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ3842636.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAERKMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            6162..8921
                     /locus_tag="WIB82_001345"
     CDS             6162..8921
                     /locus_tag="WIB82_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ3842637.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         2282 a         2933 c         2138 g         1758 t
ORIGIN      
        1 atatttgaac ctgaccgaag cataagccgc catcaaagcc ccttgcacgc cacttcgcgc
       61 cgcccgattt cctctttcaa cggcacggtg ttcaacatcc gcagacgttc ggcaaaatcc
      121 gcaaacggca gcagctccga accgaaacgg cgcatatgtt ccgtatcctg ctggcagtct
      181 atcagttcca cgcccaaatc cgccaaaaac ggcacggcgc aggcaaacgc gattttcgac
      241 gcatccggtt gcaacgcaaa cattgattcg ccataaaaca ccctgccgat ttgcacgccg
      301 taaaagccgc ccgccaacct cgtttcaccg ctttcatcgg gataatggca ctcgaacgaa
      361 tgcgcgtgtc ccatttcgtg cagcttcaaa tatgccgtct gaaactcggg cgcaatccaa
      421 gttccgtcct gattcgggcg cgccgctgcc gcacaatgcg cgaccacttc cgcaaaacag
      481 ccgttgaccg caacccgata gctgccgttg cgcagcgttt tcgccagcga gcgcggaacg
      541 tgcagacgtt cgggaacaat caccgcacgc ggaccgaccg cataccaaaa aaaccaaccg
      601 tcccgagaaa accacggaaa cacgccgttc cgatacgcct caagcagccg ccccgtatcc
      661 aaatcgccgc tcacgccgac cagcccgtcg caccgggcca aagcataggc aggatcggga
      721 aaggcataat tgtcaggggc aagcagcgga atacgcatgg cagttctcca aaaacacaga
      781 cggcatccaa aatcatgaat gccgtctgaa caacaaaccg tccggattta acgtttgccc
      841 ttggcctgac agtcgctgca cacgccgtac atataaagcg cgtgatcgac gatgcggtag
      901 ccgttttctt ccgcgatttt gtcttgcagg gcttcgattt cgggattgtg gaattccgtt
      961 acctcgccgc acttcacgca gacgatatgg tcgtggtggt cgcccttgtc caactcataa
     1021 accgccttgc ccgtttcaaa atggtggcgt tgcagaatac ccgcctgctc aaactgggtc
     1081 agcacacggt aaatcgtcgc cacaccgatt tccacaccct cttccaacaa aatgcggtac
     1141 acatcttccg cactcaaatg ctcttccgca tgcgtctcga acaaatccaa aatcttcaaa
     1201 cgcgggccgg taaccttcag accgctgtct ttcagttgtg caatattgtt gaatttttcc
     1261 ataatattca atacccctgt aaaacaatag acgttataat acgcaatttc ggcctgtttg
     1321 cccactatcg caccatagca gtttgcaata gtaaaaccgc accggcggcc gatgcgcgga
     1381 cagggcggat accgcttaat cgtatgatta tcgttcgctt ttataaaata ttcaagcagt
     1441 gttacactac ccatcccgat ttgcacagaa aggcatttcc gtgaacaaaa ccctcatcct
     1501 cgccctttcc gccctcctcg gccttgccgc gtgcagtgcc gaacgcgttt cactgttccc
     1561 ctcgtacaaa ctcaaaatca tacagggcaa cgaactcgaa ccgcgcgccg ttgccgccct
     1621 ccgccccggc atgaccaaag accaagtcct gctcctgctc ggcagcccca tactgcgcga
     1681 cgcattccat accgaccgct gggactatac cttcaacacc tcccgcaacg gcatcatcaa
     1741 agaacgcagc aatctgaccg tctattttga aaacggcgta ctcgtccgca ccgaaggcga
     1801 cgtcctgcaa aacgctgccg aagccctcaa agaccgccaa aacacagaca aaccataagg
     1861 aacacacatg acaccgctca aaatcgccat cgccggcgca aacggccgta tgggacgcgt
     1921 attggttgaa gccgtcaaca accatcccga caccgtcctt tccggcgcgc tcgaacactc
     1981 gggctcagaa gccctcgggc tggacgcagg ctacgccgtc ggactcaaaa ccggcatcgc
     2041 catttcagac gacgttgacg ccgttctcgc acaaagcgac gtactcatcg acttcacccg
     2101 ccccgagccc accctcaaac acctgcaaaa atgcgttgaa aaacaagtca acatcatcat
     2161 cggcacaaca ggcttcgacg atgcgggcaa agccgctatc cacactgccg ccgaacaaac
     2221 aggcatcgtt ttcgccgcca acttcagcgt cggcgtcaac ctcaccttcc acatcctcga
     2281 caccgtcgca cgcgtactaa acgaaggcta cgacatcgaa atcattgaag gccaccaccg
     2341 ccacaaagtc gataccccca gcggcaccgc gttacgcatg ggtgaagtca tcgccggcgc
     2401 gctcggccgc gacctcaaac aatgcgccgt ttacggccgt gaaggccaca ccggtccgcg
     2461 cgatccgtcc accatcggct ttgccaccgt ccgcgcaggc gacatcgtcg gcgaccacac
     2521 cgccctcttc gccaccgacg gcgaacgcgt ggaaatcacc cacaaagccg gcagccgcat
     2581 gacctttgcc gccggtgccg tccgcgccgc agtttgggta aacggcaaaa cgggtttgta
     2641 cgatatgcag gacgtactcg ggctgaacaa ccgttaaccc ccatacaaaa tgccgtctga
     2701 aaagatattg ttcagacggc attttgccaa caggctccgt atcggcatat caataaagtt
     2761 gcctgcgcag catgacaagc tacggctcgt ttaattgaaa ctcttctgat ctaaaaattc
     2821 taactctatt tcccggcaaa ctatatctat actaaaacca gttagaagat cacatctacc
     2881 gcctatcaat tccagcgtac ctccataatc caagatttca tcatcctgct ttaattcgcc
     2941 aagctccaat atattatcga tatctttatc ttggctcttt gtagcgtatc catagataaa
     3001 tttcagatag ctgaactgct tgaatttaat ttttacccag tctgtataat ttggtgtagg
     3061 aatctcttga ggaaagtccg gataatccac attctggacg atgatatgga attcatcgtt
     3121 tttcaaagta tagtttaata gagtgccctc atatatatac agaaattgat aatctttttt
     3181 tggaatggta taagttgtca tattatttta ccttttcaaa aacaatgagt ttaagatttt
     3241 ttgagtccaa gttggttcta ctactcttat gaaatttcag ttgtttaaac tcttccttta
     3301 acggcccatt atattttaac cttaatccca ctcgagccag ttctccttca tttggaattc
     3361 ctcttaaata cttattgttt gatgtgccat taatcacaat ttctccccct tcttttgtaa
     3421 tacgtgctgc ttcaagaaga taatccatta accgctcaac atcccgcctc ttccaaaacc
     3481 gcgcgcgcgg ccaaatccgc cgtatctttt ttcaacagca ggtgcaacac cctgaaatcc
     3541 tgttgcagcg cggcaacctt atcggggtgt tcgtaccagt ccgccaacgc cgccgccagt
     3601 ttttccggta cggcatcatg ttgaagcagc tccggcacag cctccttacc caacaggata
     3661 ttcggcaggc caacatgcgg cactttgatt ttgcgtttca cataagcata agtcagcggc
     3721 gaaatcttgt agctgatgac catcggacgc ttgcacagcg ccacttccaa agtcgccgta
     3781 ccgctcgtta ccagcaccgc atccgccgcc ctacacaccg tttcagactg tctgtcggtt
     3841 accgtcagcg gtaatccggc aaactccgaa cgctgcaaaa tttccgccaa acgccgcttc
     3901 gtcgccgccg ttgcggcagg cagcaggaag cgtgcggcag gatagcgttc caacaacaat
     3961 aatgccgtct gaaaaaacac cggcgccata tagtcgattt cgctgacgcg gctgccgggc
     4021 agcagggcga atacggggat gccggcatcc acgcccaaag ttttccgcgc cgtttcacgg
     4081 tcgtcttcca agggcataag ctgcgccatc ggatgaccga caaactccgc acgtccgccc
     4141 gcatcgagat aaagctgcgg ctccatcggg aacaggcaca gcacgcggtt gacctgatgc
     4201 acgattttgc ccacgcgttc ccgccgccac gcccacaccg acggactgac ataatgcagg
     4261 gtcggaatgc ccgcccgttt gagccgctcc gccacaccca gattaaaatc gggcgcatcg
     4321 atgccgacaa acacatcagg tttcaacgac agcaaatccc gtaccagccc cctgcgtatc
     4381 cgtaaaattt ccggcaggcg ttttaccacc tcggcaaagc cgcgcaccgc cagtcgctcc
     4441 tgatcataaa ggctctcgaa accttccgcc ttcatcagtt cgccgccgat accgacaaaa
     4501 cgcgcctgcg ggcaacgctt tcggatggcg cgtatcaggt gcgcccccaa caggtcgccc
     4561 gacgcttcgc cgacactgac ggcaatcaaa gggctttttt tatcaaccat attcgtctgt
     4621 ccccacatac tttcacgtcc cgatgccgtc tgaaggcttc agacggcatc agggttcaca
     4681 ttttcgtccc gttttccaac atcaccgcaa actgcgccaa gtccggcggc agctccgcct
     4741 tcaacaccag cggctcaccc gtgagcggat ggttcaagtg cagctcggac gcgtgcaaaa
     4801 acatccgctt caaacccaac ttctgcaaac gacggttcgc ctgataatcg ccgtagcgtt
     4861 cgtcgcccgc aatcggacag ccttgagatt gcaggtggac gcggatttgg tgcgtgcgcc
     4921 ccgttttcaa cgtcgcccgc accaaagtca ggtgcgacag cccgacaccg tgcaaaatgc
     4981 cgtctgaaaa acggcttaac acacggaaca ccgtatgcgc cgactgcccg tccgcactga
     5041 cgcgcaccat cttttcgcct tgtgcgccgg tatatttgaa caggggcagt ttgacatgga
     5101 aattgtcgtc cggcagtttg cccaccccca gcgcaaggta gatttttttg gggtggtcgt
     5161 tacggatggc ttcgtgaagt ttgacgagcg cgctgcgttt cttcgccacc atcaacaagc
     5221 cgctcgtatc cttgtccaaa cgatgaacca actccaaata cttcgcctcc ggacgggcgc
     5281 ggcgcaactg ttcgataacg ccgaaactca cgccgctgcc gccgtggacg gcaacgccgg
     5341 acggtttgtc gatgaccaaa agcgcatcgt cttcgtaaac aacgtcaaac gcacgcgccg
     5401 gtacggcggc acgcctttca gacggcatct tgcgctccgc cacgcgcaca ggcggaatcc
     5461 gcaccgtatc cccctccgca atacggctgt cgggtttgca gcgtttcttg ttcaaccgca
     5521 cctcgccggc gcggataatg cggtggatat ggctcttggg aacacccttg aggattttta
     5581 tcagatagtt atcaaggcgt tgacccgcct catgttcggc aacccctatc aagctgaccg
     5641 aatctttgct tatttcgtgc gttttcatct ataatccgaa catccgtttc agcaaaaagc
     5701 gcgcccgccg cgcccccctg aaacggttta ctttaaaacg gaattttata ttaaaaacgc
     5761 actccgcaaa gcattttccc tgcgcctgtt cccagccggc aggcgcagaa cgtagtcaag
     5821 tttgaattga ttttgccgtt tcggcgcgga agtaagacgg cagccgggcc ccaagtccca
     5881 aacgcaaacc gcccaaaaca caggcatcac gataacaaga agaagccggc ccccattttt
     5941 ccgaccgcag cgttcggaac gcggcagaca aaccgccgca tcgatcttta tagccttctg
     6001 tatccgaccc ttccgcacac ggttctccgg aacgtgcatc cctgcggatt ttcaactcaa
     6061 aaactgatta ccggttttat ccgaaaacat cggaaaaccc agccttacgg aatgccgaac
     6121 cggacaggtg cgcccgaatg ccgcccgtcc acgaggtgat catgaaaaga atgttattca
     6181 acgcaacgca ggccgaagag ctgcgcgttg ccatcgtcga cggacaaaac ctgctggatt
     6241 tggatatcga aacgctgggc aaagaacagc gcaaaggcaa tatctacaaa ggcatcatta
     6301 cccgcatcga gccgtcgctg gaagcgtgtt tcgtcgatta cggaaccgac cgccacggct
     6361 tcttgccgtt taaagaagtc tcccgctcat acttccaaga ctacgaaggc ggacgcgcgc
     6421 gcatccaaga cgtgctcaaa gaaggcatgg aagtcatcgt ccaagtcgaa aaagacgagc
     6481 gcggcaacaa aggcgcggcg ctgaccacct tcatcagcct cgccggacgc tatctggtat
     6541 tgatgccgaa caacccgcgc ggcggcggcg tatcccgccg tatcgaaggc gaagaacgtc
     6601 aagaactcaa agccgctatg gcgcaactcg acattccgaa cggcatgagc atcatcgccc
     6661 gtaccgccgg catcggccgc agcgcggaag agttggaatg ggatttgaac tatctcaaac
     6721 aactctggca ggcgattgaa gaagcaggaa aagcgcatca cgacccttac ctgctcttta
     6781 tggaaagctc gctgctcatc cgcgccatcc gcgactattt ccgccccgac atcggcgaaa
     6841 ttctggtgga caatcaagaa gtttacgacc aagttgccga gttcatgagc tacgtcatgc
     6901 cgggcaatat aggccgtctg aaactctacg aagaccacac gccgctgttt tcccgcttcc
     6961 aaatcgaaca ccaaatcgaa agcgcgtttt cgcgcagcgt cagcctgccc tccggcggcg
     7021 cgatcgtcat cgaccatacc gaagccctcg tctccatcga cgtgaactcc gcacgcgcca
     7081 ctcgcggcgc agacattgaa gacaccgcgt tcaaaaccaa tatggaagcc gccgaagaag
     7141 tcgcccgaca aatgcgcctg cgcgacttgg gcggcttggt cgtcatcgac ttcatcgaca
     7201 tggaaaaccc caaacaccag cgcgatgtgg aaaacgtcct gcgcgacgcg ctcaaaaaag
     7261 accgcgcccg cgtacagatg ggcaaactct cccgtttcgg acttttagaa ttgagccgcc
     7321 aacgtttgaa accggctttg ggcgaaagca gccacgtcgc ctgtccgcgc tgcgccggca
     7381 ccggcgtgat tcggggcatc gaatccaccg ccctgcacgt tttacgcatc attcaagaag
     7441 aagcgatgaa ggacaacacc ggagaagtgc gcgcacaagt gcccgtcgat gtcgccacct
     7501 tcctgctgaa cgaaaaacgc gccgagctgt ttgcgatgga agagcgtttg gatgtcaacg
     7561 tcgtcctgat tccgaacatc cacctcgaaa atccgcacta cgaaatcaac cgcatccgca
     7621 ccgacgacgt agaagaagac ggcgaaccga gctacaaacg cgtcgccgag cctgaagaag
     7681 acgaatccgc caaaccgttc ggcggcgaaa aagccaaagc cgcccgtccc gaacccgccg
     7741 tcaaaggcgt gcgccacacc agccccgccc cgactgccgc ccccgagaaa aaaacctcct
     7801 ggtgggacag cttcaaagct tggctcaaac gcattttcgg cggcagcgaa acccaagccg
     7861 cgcccgctgc cgaaacctcc gaaaaacgca gcacggcaaa ccgcagcggc agccgcgcca
     7921 acaaccgccg ccaaaacccg cgccgcagca aacgcgaagg cagcaaagta gaagtccgcg
     7981 aagtggcagg caaaactgcc ggacaggaag cgcgtgccga caaagccgaa acgcgcaaca
     8041 acggcaaccg ccgccgcaac gaacgcggcg accgtgccgc cggacgcgcc gacgaagcgg
     8101 aaatccaaga ccgcaacata cagcctgccg caaccgttgc agatgccgca ccgtccgaaa
     8161 ccgaagtgca aaccggaaaa cgccgccgca acggcagccg cagcgaacgc ggccaaaccg
     8221 cgccggaaac cgccaccgtt gccgaaacaa ccgttcagac agcggaaaac acgccgtccg
     8281 aaccgcatac cgcagaagac aaaggcagca agcccaaatc cgaacgcaac cgccgcgaac
     8341 gcgacagccg agatgccaaa gaacgccgcg agcgcaacaa tcagcgcgac cgccgtcaaa
     8401 acggcaaaaa acgcaatatt ccgtctgccg ccaaaatcga gcagtacctg aatattcacg
     8461 acaccgccga caaagtccgt tccgccgccg cgcacgtttt cggcgaaacc gacgcaaacg
     8521 cgccgattac tgtcagcatt gccgatccgg ttgcagaaag ggatcttccg acagcatctc
     8581 ccgccgtttc aaacggcgac gcaccggttt atgatgcggc ggaaaaaatc cgccgtgcca
     8641 ccgccgccat cctgcccgaa ggcgcgacac cgaaagccga agcacaggaa atgccgtctg
     8701 aaaccgcaac ctttacggct gcggcggaac aggcacggga aaccgcacaa accggcggac
     8761 tcgtcctgat cgaaaccgac cctgccgcat tgaaggcatg ggcggcacaa cccgaagtcc
     8821 aagccggacg cggtttgcgc cgttccgaac agcccaagcc gtctgaagtc gcaaccgtcc
     8881 ctgccgaaga aatgatccaa gtcgaaaccc ggcaaggctg aaccgacggc ggcaaaaaga
     8941 ggttctgttc cgcagaacct cttttttaca tgggttcgga tacctgcaat gccgtctgaa
     9001 acttcgccat tcccgtgatt accgaaacat tccgccattc ccatgattcc cgcaacattc
     9061 cgtcattccc atgattcccg caacattccg tcattcccat gattcccgca a
//
LOCUS       DAWHRS010000082         8891 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWHRS010000082 DAWHRS010000000
VERSION     DAWHRS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.082"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..287)
                     /locus_tag="WIB82_001346"
     CDS             complement(<1..287)
                     /locus_tag="WIB82_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3842638.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQS"
     gene            526..1425
                     /gene="crgA"
                     /locus_tag="WIB82_001347"
     CDS             526..1425
                     /gene="crgA"
                     /locus_tag="WIB82_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ3842639.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1653..4868)
                     /gene="carB"
                     /locus_tag="WIB82_001348"
     CDS             complement(1653..4868)
                     /gene="carB"
                     /locus_tag="WIB82_001348"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ3842640.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDVIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAMNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECEARPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4908..5519)
                     /locus_tag="WIB82_001349"
     CDS             complement(4908..5519)
                     /locus_tag="WIB82_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842641.1"
                     /translation="MINIIATWAMALTYIFLLLFIIVFIPIQLYLENIKKKKFKSRII
                     DILKNNHNNLDLNDIKQMTEAVNLNNFAARKIIKQLYYTDELNLNLVRQLQKEIQQEE
                     PFDGCSDELKPTLIGINELLETHGSESQKHLLTPIISELKELNQIKHDHKKMKTQSYI
                     AYIIAIISFFIGSISFYYTITAPSGKEIANTVVEQLKANQNQQ"
     gene            complement(6367..6756)
                     /locus_tag="WIB82_001350"
     CDS             complement(6367..6756)
                     /locus_tag="WIB82_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ3842642.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7329..7454)
                     /locus_tag="WIB82_001351"
                     /pseudo
     CDS             complement(7329..7454)
                     /locus_tag="WIB82_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7500..8633)
                     /gene="carA"
                     /locus_tag="WIB82_001352"
     CDS             complement(7500..8633)
                     /gene="carA"
                     /locus_tag="WIB82_001352"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ3842643.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIKAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         1952 a         2245 c         2350 g         2344 t
ORIGIN      
        1 tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta ttttttcact
       61 gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacagcatc catccaaacg
      121 aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc ttgaacattg
      181 tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt taacccgccg
      241 tgagaatgtc cgaacgcttt gccgccgtct aataataaaa tattcatttg ttacctcgtt
      301 tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc aaaaataaga
      361 aaacactttt gcgtcaaatg aaataatcag atgttccaac acacgggatg gcacataaag
      421 caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa taaagtctga
      481 tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa aaccaattca
      541 gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg tgcggcggag
      601 cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga ggaaaagttg
      661 ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga aggcgcgcaa
      721 tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac cgaaatgctg
      781 gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc gatggtgctg
      841 catctgctgg cgccgctggc aacaaaattc aacgaacgct atccgcatat ccgactttcg
      901 ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat tgccttacgg
      961 gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag ccgcttccgc
     1021 gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctac agaagagctt
     1081 gccggccacc aatgtttagg cttcaccgaa cccggttctc taaatacatg ggcggtttta
     1141 gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag cggtgaaatc
     1201 ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt tttggttgac
     1261 aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc cgataaaaca
     1321 caccccttta atgctgttta ttacagcgat aaagccgtca atctccgctt acgcgtattt
     1381 ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta taaaaacctg
     1441 ccataaaatt gtttttactc acttaataac tttgagaaaa aataaataag atgatgtata
     1501 gccgcggcaa tggcagtcta tatggggagc gtgatagggc tgttccttat gtacggcttg
     1561 tgtgtggcaa catcggcttt ccaaatgaca aaatgccgtc tgaaaccgta aaaaaaaagg
     1621 tttcagacgg cattttcaac ctgattcagg catcagttgc ggtttttcaa acgcccgtgc
     1681 agttcttgaa cgctgtacac gcccaagtgg tcgcggcttt tcgcgccttc gctcatcgct
     1741 tcgccgccgg cggtggtggt gtattgcggc acgcgttgct gcaatgcgct ttggcggatg
     1801 atgtggctgt cggacacgga ttgcggatcg ctggaaacgg tattcacgac cagtgcgatt
     1861 tcaccgtttt tcagcgcgtc gccgatgtgc gggcggcctt cggggacttt attgatggtt
     1921 tggacaatca gcccgtgttc ggtcaggtat tgcgccgtgc cgcgcgtggc gcagatgccg
     1981 tagcctaagg cttggaagtt tttggcggtt ttaatgacgc gttctttgtc ttcttcgcgc
     2041 acggagagga agattttgcc ggtcgggttg aggcgttcgc ctgcgccgag ttgggctttg
     2101 tagtaggctt cgccgaagct tgcgcccacg cccatgactt cgccggtgga gcgcatttcc
     2161 ggacccaaaa tcgtatccac gcccgggaat ttgatgaagg ggaacacggc ttctttaacg
     2221 gcatagaaat cagggacgac ttctttttcc acgccttgtt ctttcaggga aatgcctgcc
     2281 atacagcgtg cgccgacttt ggcgagcggc acgccggtgg ctttggagac gaaggggacg
     2341 gtgcggctgg cgcgcgggtt cacttccaat acgaacacta cgccgtcctg cacggcaaac
     2401 tgcacgttca tcagtccgac cacgcccagc gcgtacgcca tcgctttggt ttggcggcgg
     2461 atttcgtctt ggatttcttc gcttaaggag tagggcggca gcgagcagcc ggagtcgccg
     2521 gagtggatgc ccgcctgttc gacgtgctgc atgatgccgc cgataaccac atctttgccg
     2581 tctgaaacgc agtccacatc gacttcaatc gcgttgttca ggaagaagtc gagcaacacg
     2641 gggctgtctt cggaaacctg cacggcttcg cgcatatatt tttgcaactg ctcggcagag
     2701 tggacaatct gcatcgcgcg tccgcccaaa acataagacg ggcgcacgac cagcggataa
     2761 ccgatttctt cggctttgac gagtgcttct tcttcgttgt gggcgatgcg gttgggcggc
     2821 tggcgcaggc ctaagtcgtt caacactttt tggaagcgtt cgcggtcttc ggcggcatcg
     2881 atgctgtcgg cggacgtgcc gatgatgttc acgccgtttt caaccagcgc gttggcgagt
     2941 ttgagcgggg tttgaccgcc gtaatgcaca atcacgcccc acgggttttc ggtgcggacg
     3001 atttccaaca cgtcttccaa tgtcagcggc tcgaaataga ggcggtcgct ggtgtcgaag
     3061 tcggtggaca cggtttctgg gttgcagttg accataatcg tttcaaaacc ggattcgcgc
     3121 agggcgagcg cggcgtgaac gcagcagtag tcaaactcga tgccctgacc gatgcggttc
     3181 gggccgccgc cgagaatcat cacttttttg cggtcggaag gacgcgcttc gcattcctct
     3241 tcgtaagtgg aatagaggta ggcggtttcg gtggcgaact cggcggcaca ggtgtcgacg
     3301 cgtttgtaaa ccggatgcag cttcagcgcg tagcggtgtt cgcgaacttc tttttcgctt
     3361 acgttcaaca attgtgccaa acgtttgtcg gagaagcctt tgcgtttcag acggcgtagg
     3421 gcggcgaaat ccaaatcact caaaatgccg tctgaaaccg ctttttcttc cttcatcaag
     3481 tcttcgattt gcgccaggaa ccaagggtcg atggcgcaga tttcgtggat ttcttccagc
     3541 gtgaagccgg cgcggaacgc gtctgccaca aacagcatac gctcagggcc ggggttcgcc
     3601 agttcgcggc ggatttccgc tttgtcggag cttctcggat tgaagccgca caagccggtt
     3661 tccaagccgc gcaaggcttt ttggaagctt tcctgaatcg tgcggcccat cgccatcact
     3721 tcacccaccg atttcatctg tgtggtcagg cggtcgtctg cggcagggaa tttttcaaac
     3781 gcgaaacgcg ggattttggt tacgacatag tcgatggacg gctcgaacga cgcgggggtt
     3841 ttgccgccgg tgatgtcgtt gcgcaactcg tccagcgtaa agccgaccgc cagcttggcc
     3901 gccaccttcg caatcgggaa gcccgtcgct ttggaagcca gcgcggacga acggctcacg
     3961 cgcgggttca tctcaatcac aatcatctcg ccgttttcag ggttcatcgc aaactgcacg
     4021 tttgagccgc ccgtgtccac gccgatttcg cgcaataccg ccaacgaagc gttgcgcatg
     4081 atttggtatt ccttgtccgt cagcgtttgc gccggcgcga ccgtaatcga gtcgcccgta
     4141 tgcacgccca tcgggtcgaa gttttcaatc gagcagatga tgatgcagtt gtcgttcttg
     4201 tcgcgcacta cttccatctc gtactctttc cagccgagaa cggactgctc aatcagcagc
     4261 tcgtgcgtgg gcgaggcatc gaaaccgcgt tcgcaaatcg ccaaaaactc gtctttattg
     4321 taggcaatgc cgccgcccga accgcccatc gtgaaagaag gacgaatcag ggtagggaag
     4381 ccgacctgtt cctgcgccgc caaagcttcg ttcattgtgt ggcagacaaa agatttcggg
     4441 caagacaaac cgattttttc catcgcctct ttaaagcggc cgcggtcttc tgctttgtcg
     4501 atgacgtctt ccgtcgcgcc gattaattca acattgtatt tcgccagcac gccgttgcgc
     4561 gccaaatcca gcgcacagtt cagcgcagtt tgaccgccca tggtaggcag aatcgcatcg
     4621 ggccgctctt tggcaataat tttttccacc gtctgccaca taatcggctc gatgtaggta
     4681 acatccgcca tttcggggtc ggtcataatc gtggcggggt tggaattcac caaaatgact
     4741 ttatagcctt cttcacgcaa ggctttgcag gcctgtgcgc ccgaatagtc aaattcgcag
     4801 gcctgaccga taacgatagg gccggcgccg atgataagga tggattttag gtcggtacgt
     4861 ttgggcatga tgggtctttc tatgaaatca aatgtttagc taaaaattta ttgctgattt
     4921 tgattggctt ttaattgctc tacgacagta ttggcaatct cttttcctga tggggctgta
     4981 attgtgtaat aaaagctaat actgcctatg aagaaactga taatggctat gatataggct
     5041 atataacttt gtgttttcat ttttttgtga tcatgtttga tctgatttaa ttcttttaat
     5101 tcagaaataa ttggagttaa taaatgtttt tgactttcag atccatgcgt ttccaaaagt
     5161 tcatttattc ctattagtgt tggttttagt tcgtctgagc agccatcaaa aggttcttct
     5221 tgttggattt ccttttgtaa ctgtctaact aaatttaaat ttaattcatc agtgtaatat
     5281 agttgcttaa taatttttct ggcagcaaaa ttattaaggt ttactgcttc tgtcatttgt
     5341 tttatatcat taagatctag gttattgtga ttatttttta atatatcaat tattctagat
     5401 ttaaatttct ttttctttat attctctaaa tatagctgaa ttggaataaa aacaattata
     5461 aagagcaaaa gaaatatata tgttagtgcc atagcccatg tggcaattat atttatcatt
     5521 tatcaatatc ttccatttgg tttatatatt gtctcataat aagcaagccg tagctcgcat
     5581 gcagggtgtg tgcggtacgc acgcgttctc cattttccct gcaaccccaa cccaccgtgt
     5641 gcgtgccttg cggcacacac cctaccgatg ggttcaaagg tcgtctgaaa aaacgctcgc
     5701 ggcgtttgtt ccatctgcct tgcgataaag gctgcctgaa aaattcccgc tgttgatatt
     5761 tcgggcattc tttctcaaac cgcctgcaac cgcaaaatcc gctgcaacct gccccctccc
     5821 ccgtggggga gggttgggga gagggcattc cccgaatttc ggcaaccttt cccaacaact
     5881 caaccaccga aatacaagcc ttgtggcttg ttgccctctc tctagctctc tcccacggga
     5941 gagaggacgg agtggctgta gggctaaggg ttctgcaagg aaacaggttg tgcaggttgc
     6001 tttttgcttt tcagacgacc tgaaatcaac ggtaaatcca agtcgctttt caaaccgcct
     6061 gcaaccgcaa aatctgctga aacctactcc ctcccccgtg ggggagggct ggggagaggg
     6121 cattctccga atttcggcaa cctttcctaa cactctaacc gccaagacac aagccttgcg
     6181 gcttgttgcc ctctctctag ctctctccca cgggagagag gatggggtgg ctgttggggc
     6241 taaggtttct gtaaactaat caggttatgc aggctgcttt ttgtttttca gacggccttt
     6301 ataaatgctc aattcagtcg gctttatcct attattttca ataatccaat caaaatcagc
     6361 catctgctat cgtgcagctt gcttttccaa ttcttgcaac acgagcagga tttctgtcag
     6421 cacatcgttt gtctgctgta aaatctcgtg attccaaaac cgcagtacgg taaagcccaa
     6481 gctgttgaga tatgccgtcc gcgcgtggtc gtatgcggct tgttctgtgt gctgccctcc
     6541 gtctgcttcg acaatcaact tgggcgttac gcacataaaa tcaacaatat agtttcccat
     6601 cggctgctgg cggcggaatt tatagccgtt cagacggcct gcccgcaggt gctgccacaa
     6661 ttttgcttcc gcctcgctca tttcttggcg catggctttg gcgcgttggc gcaagatggg
     6721 gttgtcggtg gtcaagagtt tctcgtgcgg gttcattttg ttgtcgtgct gtttgctgcc
     6781 ctctctctaa ctctctccca cggggagaga ggacggggcg gttgttgggg ttaaaggggc
     6841 tgtaaggaaa acaagtcgtt caggccactt tttacttttc agacgacctg caatcaacgg
     6901 ttgtaaatcc aagttgcttt tcaaaccgcc tgtaaccgca aaacttgctg caacctaccc
     6961 cctcccccgt gggggagggc tggggagagg gcattctccg aatcgcggca atctttccct
     7021 aattccctaa ctgcctcaat acaagccttt cggcttgttg ccctctctct aacgctttcc
     7081 cacgggagag aggacggtgc ggctgtagtg attaagggtt ttgcaaggaa aacaaggaaa
     7141 acaagttgta caggctgttt tttatttttc aagcgacctg aaatcaacgc ttgtaaatcc
     7201 aagtcgcttt tcaaactgcc tgcaaccgca aaatctgctg caacctaccc cctcccccgt
     7261 gggggagggc tggggagagg gcattttcca agttgcggca atctttccca aatcccttag
     7321 tcttctaaaa tacaagcctt acggttgttg ccaaatgaaa cttcttccat acccaaaact
     7381 tgcgtgtaaa acgcaatggt tcggtcaatg tcggcaacgg tcagtaccag atggtcgagt
     7441 gcgctaatct tcatttcccg ctttcttttt caggcggcgg cagtgccgtc tgaaagccat
     7501 tatgcccgtt ttgccgcttt catattgcca atgaatttgt caaacaaata gccgacatct
     7561 tgcggaccgg ggctggcttc ggggtggcct tggaagcaga acacaggttt gtcggtcagc
     7621 tcgatgcctt gcaaagtgtt gtcaaacaag gatttgtggg taatgcgtgc gttagcgggc
     7681 agggtgtcgg catcaacggc aaaaccgtgg ttttggctgg tgatgacgac tttgccgctg
     7741 tccaaatctt gcacagggtg gttcgcaccg tggtggctga agcgcatttt cagggttttc
     7801 gcgccgatgg cgaggctgat gagctggtgt cccaagcaaa tgccgaaaat cggtttgccg
     7861 ctttccatca gtttttgtac ggctttgatg gcgtaggtgc aaggctcggg gtcgccgggg
     7921 ccgttggata ggaatacgcc gtcagggttg agtgccaaca cgtcttccgc gctcgtttgg
     7981 gcggggacga cggtcaggcg gcagccgcgc gaggcgagca tacgcaggat gttggttttc
     8041 acgccgaaat cgtaggcgac gacgtggtaa ggctgttcgt caggggtaac gaaacccttg
     8101 cccaatgccc attcgccttc cgtccattcg taagtttccg tgcaggaaac ttcttttgcc
     8161 aagtctttgc cgaccatact gccgaacgcg gcgatgagtt cttgcgcttt ttcgattgtg
     8221 gcatccgcac cggtcagaat cgcgccgcct tgcgcgcctt tttcgcgcaa cagcgtggtc
     8281 aggcggcggg tatcgatgtc ggcgatggcg acggttttgt tgcgtaccag atagtcgtgc
     8341 aggctttcgg aggcgcggaa gttgctgtgc aagagcggca ggtcgcggat aatcaggccg
     8401 gcggcataaa cgctgcggct ttcttcatct tcggcgttgg tgccggtgtt gccgatgtgt
     8461 gggtaggtga gggtaacgat ttgtttgcag taggacgggt cggtcaggat ttcctgatag
     8521 ccagtcatcg aagtattgaa cacgacttcg ccggaagtcg aaccttcgta accgattgat
     8581 gtgccgtgga atacgctgcc gtcagcgagg acgaggaggg cgggggtgct catgatggga
     8641 atcctgtttt taataaaatt cttgacaatg ccgtctgaag ggacttcggc agggggcatc
     8701 cggtcggaaa aatccggtca aaaaaaaaca cgccgcgcaa gatagacgcg taacgtgctt
     8761 ttcgggcaag tgcctatacc ctgaaagcag catattttaa gacgaaacat acccgatttc
     8821 aagcgcaaac gcggcaggcg ggctgatatg ttcgcgcgaa aacggaaatc cgtggcgtaa
     8881 aaccgcccgc t
//
LOCUS       DAWHRS010000083         8767 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWHRS010000083 DAWHRS010000000
VERSION     DAWHRS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.083"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            239..1774
                     /locus_tag="WIB82_001353"
     CDS             239..1774
                     /locus_tag="WIB82_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ3842644.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLNIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     DEK"
     gene            1771..1863
                     /locus_tag="WIB82_001354"
     CDS             1771..1863
                     /locus_tag="WIB82_001354"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ3842645.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1923..3143)
                     /gene="lysA"
                     /locus_tag="WIB82_001355"
     CDS             complement(1923..3143)
                     /gene="lysA"
                     /locus_tag="WIB82_001355"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ3842646.1"
                     /translation="MTLFCEQVPYPRLAEEFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELVRVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAERLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDTLVA
                     EGIVLEHLDLGGGVGIVYQDESVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGGGYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3154..3324)
                     /locus_tag="WIB82_001356"
     CDS             complement(3154..3324)
                     /locus_tag="WIB82_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ3842647.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSAPQTQK"
     gene            3395..3718
                     /gene="cyaY"
                     /locus_tag="WIB82_001357"
     CDS             3395..3718
                     /gene="cyaY"
                     /locus_tag="WIB82_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ3842648.1"
                     /translation="MMTESEFIRMSEELFEHIEDQIDENGWDFDCQFAGNVLSIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3745..4761
                     /locus_tag="WIB82_001358"
     CDS             3745..4761
                     /locus_tag="WIB82_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ3842649.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4787..5206
                     /locus_tag="WIB82_001359"
     CDS             4787..5206
                     /locus_tag="WIB82_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ3842650.1"
                     /translation="MAQLPLYRTAEIKDFTVGTPEVLQSFFEHVPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTLAALCINDYIHAVYDFVAQ
                     AGYCRNGFPEAQGEWLKVADRVLDDELLDKILSRKPT"
     gene            5228..5734
                     /gene="luxS"
                     /locus_tag="WIB82_001360"
     CDS             5228..5734
                     /gene="luxS"
                     /locus_tag="WIB82_001360"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ3842651.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVANVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5880..8696
                     /gene="polA"
                     /locus_tag="WIB82_001361"
     CDS             5880..8696
                     /gene="polA"
                     /locus_tag="WIB82_001361"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ3842652.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESRMHEAADGDLFGSDSIGEQAALNAEVPSEKQPELAPTPEKLDYQA
                     VTTEAQLAALLDKLSQADTIGIDTETTSLDAMNASLVGISIAFQAGEAIYIPVGHSLT
                     AAPEQIDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELSRQSAELGAELMKLE
                     QEAYAVAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTEEGRKVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAK
                     VDGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         2054 a         2274 c         2307 g         2132 t
ORIGIN      
        1 tcctgcggga ttggtgtggg atttagggct aatctagtac agcccctaaa atttttcgtt
       61 ttcaaacctt caccgcttgc catcagcgtt aaattttttt acgataagca catagattgt
      121 aaacaatcgg ccacaagccg gtttgttttt tcagaagaca ttatccctgt cagacgctgt
      181 ttctatatat ttcgcctata atggcttgtt tttaataaat aattcaagag gtatcaacgt
      241 gtctgattcc aagacgaaag aacgcgccac attcggcacg cgccgcgcgt ttatgattgc
      301 cgccatcggg tccgccgtcg gcttgggcaa tatttggcgt ttcccctata ttgcttttga
      361 aaacggcggc ggcgcgttca tcctgcccta tctggtcgcg cttctgacgg cgggcatccc
      421 gctgctgctg ctcgattatg ccatcggcca ccgttaccgt ggttctgcgc ccttggcttt
      481 ccgccgcctc ggacgatggt ttgagccggt cggctggtgg aacgtgatga ccaatatcgt
      541 catctgcatc tattacgcgg taattatcgg ttgggcggca agctatacct attattcggt
      601 caacgccgcc tggggtgcgg atccgcaggg ttttttcttt aaggacttcc tgcaaatggc
      661 gggcccggaa gccttgggtt tggattttgt cggcaaagtc gccggtcctt tggcgggcgt
      721 gtgggttttt accgccgcca ttatggcgtt gggcgtgcaa aagggcgtgg cgcgcgcctc
      781 gtcgttcttt atgccgctgc ttttggtgat gtttttgatt atggtcggca tttcactaac
      841 cctgccgggt gcggcaaagg gcttggacgc attgtttacg cccgactggt cgaaactcgc
      901 cgattccaag gtctgggtgg cggcatacgg gcagattttc ttttcgcttt ccatctgctt
      961 cggcattatg gttacctatt cttcttattt gaagaaaaaa accgacttgg gcggaacggg
     1021 gctggtggtc ggttttgcca acagcagctt tgaactgctc gcgggcatcg gcgtgtttgc
     1081 cgcattgggc tttatggcgc aggcgggcgg taaggcggtc aacgaggttg cctcaggcgg
     1141 catcggtttg gcgtttatcg cctttccgac cattatcaac caggcaccga tgggctggct
     1201 gatcggcata ttgtttttcg gttcgctggt attcgccggc gttacgtcga tgatttccat
     1261 ccttgaagtg attgtggcgg cgattcagga caagctgaac atcgggcgcg tcaacgccac
     1321 gctgctggtc tgcattccga tgggcattgt ttccacgctg ctgttcggta cggcgacggg
     1381 gctgccggtt ttggacgtga tggacaaatt cgtcaacacc tacggcattg ttgccgccgg
     1441 ctttgtttat gttgccgcca tcatcatcag cggcaggctg ccggaattac gcaagcacct
     1501 gaacgccttg tcctccatcc gcatcggcgg cttgtggacg gtctgcgtcg tggttaccgt
     1561 cgtgatgctc ggctatatgc tgtttaaaga taccagcggc ctgatggaga aaaattacga
     1621 aggttatccg gatggtttcc tcaatatttt cggttggggg atgtcggcgg cgttggtcgt
     1681 gttcgggctg ctgctgtcgt tgctgccttg gaaacacggt caggatttca acgtcaaaga
     1741 cgaacacgaa catgaacaag gagacgaaaa atgagtactt ccgccattgt gatgatgatt
     1801 gtctcaatcg tgataatctg gggagggctg ctgctttccc tgttaaggct gccgaacgag
     1861 taagccttta gagcgttaaa aatgccgtct gaactgtttc agacggcatt ttgatgttcg
     1921 gcttacaggc aggcgagttc gtttgccatt tgctgttcca aggtttcgcg ccggcggatg
     1981 agtcggtatc cgccgccgtc aaccaatacc tccgccgcac ggttgcgcgc gttgtaattg
     2041 ctcgccatgc tggcaccgta tgcgcccgcg ctgcggataa gcagcaaatc gccttcttcg
     2101 caggcgatgg tgcggtcttt gccgaggaag tcgccggttt cacaaatcgg gccgacgatg
     2161 ttggcggtca gcgtcgcgat gtttttggtt tcgaccgctt cgatgtggtg gtaggcatca
     2221 taaagtgccg gacgcattaa atcgttcatc gccgcatcga ccatcacaaa gtttttctct
     2281 tcgccgtgtt tgacgaattc aacgcgcgtc aataacgcac ctgcgttacc gaccaagcta
     2341 cgacctggtt cgaggatgag tttcagacgg cgtgtgccga tcagtttttg aaccgcttgg
     2401 gcatatgcgc ccaaatcggg gacgctttcg tcttgataaa cgatgccgac accgccgccc
     2461 aagtccaaat gttccaaaac aatgccttcg gcgacaagag tgtcaaccaa aatcaaaatg
     2521 cgttcgcagg cttcgaccag cgggcttaag tcggtcagtt gcgaaccgat gtggcagtcg
     2581 atgccgatga ttttcaaatt gggctgttgt gcggcatggc ggtaggcttc gagcgcgtcg
     2641 gcgtaggcga tgccgaattt gttggctttc agtcctgtgg agatgtaggg atgggttttc
     2701 gcatcgacat cggggttgac gcgcagggag acggacgcgg ttttgcccaa gcgttcggca
     2761 accttttgaa tgcggtcgat ttcgggaatg ctttccatat tgaagcattt cacgcccgca
     2821 ttcagcgcaa actcgatttc cgcctcgctt ttgcctacgc ctgaaaagat ggttttcgcc
     2881 gcgtcgccgc ctgccgccaa aacgcgtacc aattctccgc cggacacaat gtcgaaaccg
     2941 ctgcccagag aagcaaagtg tttgatgatg ctcaggttgc cattggcttt aaccgcatag
     3001 cagacgagcg gggagagctc ggcaaaggca gtttggtagt gttcaaatgc ttcggtcagc
     3061 gcggattggc tgtacacata aagcggcgtg ccgaattctt cggcaaggcg ggggtagggg
     3121 acttgttcgc aaaatagggt catgttttcg ttttcatttt tgggtttgtg gagcggattg
     3181 tggtttgctt tgaagttgca aaccggtttg gattacgccg aaacgcgcct tgtcgccttc
     3241 tttgggcagg tagaggtcgc ctttgtaacc gcaggccgag agcaggaggg cggttgccgc
     3301 cgcaaaaaat acgccgtatt tcatcggtaa acttccttca taagcgcgaa tgtggcaaga
     3361 ttcggtatct taaacaaaaa acacgcaaaa agccatgatg accgaaagcg aattcatccg
     3421 catgagcgaa gaattgttcg aacacatcga agaccaaatc gacgaaaatg gctgggattt
     3481 cgactgccag tttgccggaa acgtcttgag catcgaagcc ggagacggca cgcaaatcat
     3541 cgtcaaccgc cacacgccca atcaggaatt gtggattgcc gccaaaagcg gcggctacca
     3601 tttcgccgag caaaacggca aatggctggc aacacgcgac agccgcgatt tctacgacgt
     3661 tttaaacgaa gcactgagcg cggcttcggg cgaagcggta gagattgagg aattgtgatt
     3721 tgggtgttat cacggaaaga aaaaatgaac acacgtccct tttatttcgg acttatattt
     3781 atcgcgatta tcgctatact tgctaactat ttaggaaaca ctgatttttc ccatcattat
     3841 catatcagtg ctttaattat tgctatcttg ctgggaatgg caatcggcaa taccatttat
     3901 ccgcaatttt caacacaagt ggaaaaaggc gttttgtttg ccaaaggcgc gcttcttcgc
     3961 actggcattg tgctgtatgg tttccgcctc acttttggcg atattgccga tgtaggatta
     4021 aatgctgtcg ttaccgatgc aatcatgcta atttcaacct tcttttttac cgcactttta
     4081 ggcattcgtt atctaaaaat ggataaacaa ttggtttatc tcactggggc aggttgcagc
     4141 atttgcggtg cggcagcagt gatggcggca gagcctgtta ccaaagcaga atcccataaa
     4201 gtttcagtgg cgattgccgt agtggtcatt ttcgggacgc ttgctatttt tacttacccc
     4261 ttgttctaca cgtggtcaca acatttaatt aacgcccatc aattcggtat ttatgttggt
     4321 tctagtgtac acgaagtggc tcaagtttat gcgattgggg aaaatattga tcctatcgtg
     4381 gcgaatactg ccgtcatttc caaaatgatc cgagtgatga tgctcgcccc ctttttatta
     4441 atgctttctt ggttattaac acgtagtaat ggagtatcag aaaatacatc acacaaaatt
     4501 acaattcctt ggtttgctgt actttttatt ggtgttgcca tttttaattc ttttgattta
     4561 ttaccaaaag aactcgtgaa attattcgtt gaaatcgatt ctttcttatt aatttcatca
     4621 atggctgcgc ttggcttaac gacgcaagca agcgcaatca aaaaggcagg attaaaaccg
     4681 tttgttttag gaatactaac ttatttatgg ctagtggttg gtggattttt agtgaactat
     4741 ggaatatcaa aattaatata aaattcactc aagagagcat tacccaatgg cacaattacc
     4801 gctatatcgg actgccgaaa tcaaagactt tactgtcggc acgcccgaag ttttacaatc
     4861 atttttcgaa catgtccctt atggcgtcgt ctttgaagac gacggcgaca caggttactt
     4921 ttatgccgct tcgcaagacg ggattttaga tgccttgcac atctacaatg tcgaagatgt
     4981 atccgacaaa catatcccca atcatgtctt gatcttatgg gacgatgcct gcactttggc
     5041 agctttgtgc atcaacgact acatccatgc cgtctatgat tttgtcgcgc aggcaggata
     5101 ttgccgcaac ggcttccctg aagcacaagg cgaatggctg aaagtcgcag accgcgtctt
     5161 ggacgatgaa ttgctggaca aaatcctatc ccgaaaacct acataaacca ctcaaaagga
     5221 taacaaaatg cccctattag acagtttcaa agtcgaccac acccgtatgc acgcccctgc
     5281 cgtgcgtgtg gcgaaaacca tgaccacgcc caaaggcgac accattaccg tgtttgacct
     5341 gcgcttttgc attcccaaca aagaaatcct gcccgaaaaa ggcatccaca cgctggagca
     5401 tttgttcgca ggttttatgc gcgaccactt gaacggcaac ggcgtggaaa tcatcgacat
     5461 ttccccgatg ggctgccgca ccggtttcta catgagcctc atcggcacgc cttccgaaca
     5521 gcaggtcgcc gatgcgtggc tggcttcgat gcaggatgtg gccaatgtca aagaccaaag
     5581 caaaatcccc gagttgaacg aataccaatg cggtacttac ctgatgcact cgcttgccga
     5641 agcgcaacaa atcgcgcaaa acgtgttggc gcgcaaagtg gcggtgaaca aaaatgaaga
     5701 gctgacgctg gatgaagggc tgctgaacgc ctaatccgcc aaaaatgccg tctgaacaag
     5761 ggtttcagac ggcatttgcc ttttccgtta taatccgggg ttgtccgggg gcgggtttta
     5821 agccggcatc gtccttccct atttttttct gtcccttatc ggttttaagc gggtttttta
     5881 tgtccaacag acctacactc ctcctcgttg acggatcgtc ctacctctac cgtgcgtatc
     5941 acgcgatggg gcaaaacctg accgcccccg acggcgcgcc gacgggtgcg ctgtatggtg
     6001 tattgaatat gttgcgccgt ttgcggtcgg aatatccgca cgattattgc gcggtggttt
     6061 ttgatgcgaa aggcaaaaat ttccgccatc aaatgtttga agaatacaag gcgacgcgcc
     6121 cgccgatgcc cgacgatttg cgcccgcagg cggaagcact gccggattta gtgcgcctga
     6181 caggctggcc ggtattggtg attgggcagg tggaggcgga cgatgtgatc ggcacgctgg
     6241 cgaaacaggg ggcggaacat ggtttgcgag tcattgtttc gaccggcgat aaggacatgg
     6301 cgcagttggt ggatgagcgc gttacgctgg tgaacacgat gagcagcgaa acgctggaca
     6361 ttgaaggcgt gaaggcaaaa ttcggcgtgc gccccgacca aatccgcgat tatctcgcgc
     6421 tgatgggcga caaggtggac aacgtgccgg gcgtggaaaa atgcggcccg aaaacggcgg
     6481 tgaaatggct ggaagcctac ggttcgctgg ctggtgtgat ggaacacgct tcggaaatca
     6541 agggcaaagt gggcgaaaac ctgcaagccg cgctgcccca actgccgctg tcgtatgatt
     6601 tggtcacgat taaaaccgat gtggacttgc acgccgagct ttcagacggc atcgaaagcc
     6661 tgcgccgtac cgcgccgaaa tgggcgcaac tggttgtcga tttcaaacgc tggggcttcc
     6721 gcacctggct gaaagaagcg gaaagtcgta tgcacgaagc ggcggacggt gatttgttcg
     6781 gcagcgacag catcggcgag caggcggctt tgaatgcgga ggtgccgtct gaaaaacaac
     6841 ccgaacttgc cccgaccccc gaaaaactgg attatcaagc cgttaccacc gaagcgcagc
     6901 ttgccgcctt gttggacaaa ctgtcgcagg cggacacaat cggcatcgat acggaaacca
     6961 cgtcattaga cgcgatgaac gcctcgttag tcggcatcag catcgcgttc caagcaggcg
     7021 aagcgattta catccccgta gggcacagcc tgaccgctgc acccgaacag attgatttgc
     7081 aagacgtatt aggccgtctg aaaccgcatt tggaaaaccc cgccctgaaa aaaatcgggc
     7141 aaaacctcaa atacgaccaa cacgttttcg ccaactacgg cattgcctta aacggcattg
     7201 ccggcgacgc gatgcttgct tcctacatca tcgaaagcca cctcggacac ggcttggacg
     7261 aattgtccga acgctggctg ggcttggaaa ccattaccta cgaatcgctg tgcggcaaag
     7321 gcgcgaagca aatcagtttt gccgatgtcg ccatcggaca ggcgaccgaa tacgccgccc
     7381 aagacgccga tttcgccctg cgcctcgaag cgcatctgcg tgcgcaaatg gacgccaaac
     7441 agcttgaaat gtatgaaaaa atggagctgc ccgtcgcgca ggtattgttt gaaatggaac
     7501 gcaacggcgt gcaaattgat cgcgccgaac tctcccgcca aagcgcggaa ctcggcgcag
     7561 agctgatgaa gctcgaacaa gaagcctatg ccgtcgcagg ccagccgttt aacctcaact
     7621 cgcccaaaca gctccaagaa atcctgttcg acaaaatggg cattcccacc aaaggcctga
     7681 aaaaaaccgc caaaggcggc atttccacca acgaagccgt gctcgaacag ctcgcacccg
     7741 actaccccct gcccaaaatc atcctgcaaa accgcagcct ggcgaagctc aaatccacct
     7801 acaccgacaa actacccgaa atgatttccc ccaaggacgg ccgcgtgcat accacctacg
     7861 cccaagccgt cgccattacc ggccgcctcg ccagcaacaa ccccaacctg caaaatatcc
     7921 ccatccgtac cgaagaaggg cgtaaagtcc gccgcgcctt taccgcaccg caaggcagcg
     7981 tcatcgtttc cgccgactat tcccaaatcg agctgcgcat tatggcgcac ctctccggcg
     8041 acaaaaccct gattgccgcg ttccaaaacg gcgaagacgt acaccgccgc accgccgccg
     8101 aagtgttcgg cactgcgccc gaaaacgtct cgtccgagca acgccgctat gccaaaacca
     8161 tcaacttcgg cttaatttac ggtatggggc aatacggttt ggcaaaatca ttgggcatcg
     8221 acaacctttc cgccaaaaac tttatcgacc gctacttcgc ccgctacccc ggcgtcgccg
     8281 aatacatgca gcgcaccaaa gaacaagccg ccgcccaagg ctacgtcgaa accctgttcg
     8341 gcagaaggct ctacctgccc gacatccgca acaaaaacgc caacgcccgc gccggagccg
     8401 aacgcgctgc catcaacgcc cccatgcagg gcaccgcctc cgacctcatc aaacgcgcca
     8461 tgatagacgt gtcccgctgg ctttcagagt gcgaagcctc cccgtgggac gaactcttac
     8521 aaagcaaact gattatgcag gtgcatgacg aactggtgct ggaagtcgtt gaaaccgaac
     8581 tggattttgt caaagaaaaa ctgccgcaga ttatggcgaa agtggacggc ggattattgg
     8641 atgtaccgct ggtggctgag gttggcgtag gggagaattg ggaagaggca cattgattga
     8701 aaggtgttat atgctatctt tatttaaata aaatttaatt tttggtatat tttttctaaa
     8761 tgttcct
//
LOCUS       DAWHRS010000084         8629 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWHRS010000084 DAWHRS010000000
VERSION     DAWHRS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.084"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(78..596)
                     /locus_tag="WIB82_001362"
     CDS             complement(78..596)
                     /locus_tag="WIB82_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ3842653.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(616..2001)
                     /locus_tag="WIB82_001363"
     CDS             complement(616..2001)
                     /locus_tag="WIB82_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ3842654.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     DFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2240..3781
                     /locus_tag="WIB82_001364"
     CDS             2240..3781
                     /locus_tag="WIB82_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ3842655.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGGYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETRQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITLPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDGGEGGK
                     RTETL"
     gene            3810..4409
                     /locus_tag="WIB82_001365"
     CDS             3810..4409
                     /locus_tag="WIB82_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ3842656.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4406..4999
                     /gene="nadD"
                     /locus_tag="WIB82_001366"
     CDS             4406..4999
                     /gene="nadD"
                     /locus_tag="WIB82_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ3842657.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRAGVSDGIPPAAARYIREHGLYEK"
     gene            5058..5444
                     /gene="rsfS"
                     /locus_tag="WIB82_001367"
     CDS             5058..5444
                     /gene="rsfS"
                     /locus_tag="WIB82_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ3842658.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5498..5968
                     /gene="rlmH"
                     /locus_tag="WIB82_001368"
     CDS             5498..5968
                     /gene="rlmH"
                     /locus_tag="WIB82_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ3842659.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            6041..6307
                     /locus_tag="WIB82_001369"
     CDS             6041..6307
                     /locus_tag="WIB82_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ3842660.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6464..7147
                     /locus_tag="WIB82_001370"
     CDS             6464..7147
                     /locus_tag="WIB82_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ3842661.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVALLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7375..7887
                     /gene="coaD"
                     /locus_tag="WIB82_001371"
     CDS             7375..7887
                     /gene="coaD"
                     /locus_tag="WIB82_001371"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ3842662.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7919..8575
                     /locus_tag="WIB82_001372"
     CDS             7919..8575
                     /locus_tag="WIB82_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ3842663.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFDYQNRQIEIVAPLKNEWQTENIHRALEEFCMENKID
                     "
BASE COUNT         1890 a         2271 c         2530 g         1938 t
ORIGIN      
        1 caatgtaatc cattctgttc ccgatgttta tgccgtctga aacccatcct gtgtcgggct
       61 tcagacggca tattgcttca aagcaggttt tccgaggcaa cccagttcag aatatcggct
      121 tcgaccgctt cgggcgtgcc ggggttggcg attttgaccc cgtattcgcc ttcgcccagt
      181 tcctcgagga tttccagttg cgaatcgagc atccctgctt tcatgtaatg tcctttgcgc
      241 gacatcatgc gctcgaggtt gatgtcttgc ggcggactga ggtggatgaa ggcagctttg
      301 ccttcggctc cgcgcagaat gtcgcggtag ccgcgtttga gggcggaaca ggttacgatg
      361 gtgtggttcg caccgttttg cgcctgttgc gtcatccagt cgcgcagatt gcccaaccac
      421 ggatagcggt cttcatcggt cagcggaata cccgcgccca tcttgtcgcg gttggcttgg
      481 gtgtggaact cgtcgccttc ggcataggga cattgaccga ggtgtttctg cagggacagc
      541 gcggcggtgg tcttgccgca gccgcatacg cccataacga caaaatgcgt agtcatttac
      601 tatcctttcc gtctgtcaga cgatggcgaa cagcagtgcg gacaaggcaa agccgatgag
      661 tgcgatgagg gtttggttga ccgtccaggt tttcagcgtg gtcggtacgt ccatgtccaa
      721 gaggcggccg accagccaga agccggagtc gttgaagtgg ctgcaaccga ccgaacctgc
      781 cgccgttgcc aatacgatac aggcgagctg ccagtcggta aagtcggcgg cggcaacggc
      841 aggagccatc agcgcggcgg cggtggtcag ggcgacggtt gccgaacctt gcgcgatacg
      901 cagtgccaag gcgacaagga aacagcccaa aaggacggga atgcccaaat ccgccatgct
      961 gtcggcgagt gccttgccga tgccggaagc gcgcaaaacg ccgccgaaca taccgcccgc
     1021 gccggtaatc agaatcacgg aacagacggg ggcgagtgcg ccgtccacgg ttttttccaa
     1081 cgcgctgccg ctttcgccgc gtttgcgtcc caagacaaac agtgcgacca atacggaaat
     1141 cagaagggcg atcggtgtcg aaccgattat ttttgccgtc tgaacccagg tttcgtccgc
     1201 acttacgagt ttttcgctga tgagggccga tacgccggta ttcaggaaaa tcagcagcat
     1261 gggaatcagc atgatggcga cgaccgttcc tgctttggca ggttctttcg gcaggtcgtt
     1321 gtcttgcgtg ccgccgctga gcagttcggg aacgggaaca tggatggtgc gccccaacac
     1381 tttgccgagc atatagccgc tgaaatacca tgtgatgaag gcggtcggca gacccaaaat
     1441 caaaacttgg ccgatgttcg cgccgtaaaa ttcggaagcg gcaatcgggc ccggatgggg
     1501 cggcaggaag acgtgcatga cggaaaatgc gccgatggag gcaagcgcga agggcagtac
     1561 gtcctgtttc atgcgccgtg cggtggcgaa cacgatgggc agcatgacga ttagtccggc
     1621 atcgaagaaa atcgggaagc cgaaaatcag cgaggcaacg cccagcgcga acggtgcgcg
     1681 tttttcgccg aacatccgga tcagcgcgtc cgccagcgac tgtgcgccgc cggatgtttc
     1741 gaccagacgt ccgagcatcg cgcccaggcc gaccagaagc gccacgccgc cgagcgtgcc
     1801 gccgaagttt ttgaccagta cgtcgttgac aatgctgcct gtgggcaaac cggttgccaa
     1861 agccgtcagc aggctgacga tgaccagtgt cagcagcgcg tggatgcgga atttgacgat
     1921 taaaatcaga atgaggatga ttgccgccgc cgaaatgccc aacagggttt gcgcggacag
     1981 cgtctgtgtc cagccgtcca tttgtaaagc ctttctctgt aataaaagta caaaattgtc
     2041 aggttttttt aagttgtgcg aaaacgatag cacaaatcgg gcggtaagtt gtttgtctga
     2101 agttatattc ctgtttattt gaacgattta tttcagacgg cgggaggaag gcggatacgg
     2161 tatgtagtcg gattgaaata tacggtatgt ggggcttttg ggtacaatcg caacatcagt
     2221 gtttcaagtg gggaaggcga tggatggacg gcgttgggcg gtatggggtg cttttgccct
     2281 gctgccttcg gcttttttgg cggtaatggt cgttgcgcct ttgtgggcgg tggcggcgta
     2341 tgacggtttg gcgtggcgcg cggtgctgtc ggatgcctat atgctcaaac gtttggcgtg
     2401 gacggtattt caggcagcgg caacctgtgt gctggtgctg cctttgggcg tgcctgtcgc
     2461 gtgggtgctg gcgcggctgg cgtttccggg gcgggctttg gtgctgcgcc tgctgatgct
     2521 gccttttgtg atgcccacgt tggtggcggg cgtgggcgtg ctggctctgt tcggggcgga
     2581 cggcctgttg tggcgcggac ggcaggatac gccgtatctg ttgttgtacg gcaatgtgtt
     2641 tttcaacctt cctgtgttgg tcagggcggc gtatcagggg tttgtgcaag tgcctgcggc
     2701 acggcttcag acggcacgga cgttgggcgc gggggcgtgg cggcggtttt gggacattga
     2761 aatgcccgtt ttgcgcccgt ggcttgccgg cggcgtgtgc cttgtcttcc tgtattgttt
     2821 ttcggggttc gggctggcat tgctgctggg cggcagccgt tatgccacgg tcgaagtgga
     2881 aatttaccag ttggtcatgt tcgaactcga tatggcgggg gcttcggcac tggtgtggct
     2941 ggtgttgggg gtaacggcgg cggcagggtt gctgtatgcg tggttcggca ggcgcgcggt
     3001 ttcggataag gcggtttcac ccgtgatgcc gtcgccgccg cagtcggtcg ggggatatgt
     3061 gctgctggcg tttgcggcgg cggtgttgtc tgtgtgctgc ctgtttcctt tgttggcaat
     3121 tgttgtgaaa gcgtggtcgg ccggcgaatc gtggcgtgtg ttaatggaaa gtgaaacgag
     3181 gcaggcagtg tggaatactt tgcgcttctc ggcggcggcg gtgtttgcgg cggcggtttt
     3241 gggtgtggtg tatgcggcgg cggcgcggcg gtcagcgtgg atgcgcgggc tgatgttttt
     3301 gccgtttatg gtgtcgccgg tttgtgtttc ggcgggcgtg ctgctgcttt atccggggtg
     3361 gacggcttcg ttgccgttgc tgctggcgat gtatgcgctg ctggcgtatc cgtttgtggc
     3421 aaaagatgtt ttatcagcct gggatgcact gccgccggat tacggcaggg cggcggcggg
     3481 tttgggtgca aacggctttc agacggcatg ccgtattaca ctccccctct tgaaaccggc
     3541 gttgcggcgc ggtctgactt tggcggcggc gacgtgtgtg ggcgaatttg cggcgacatt
     3601 gttcctgtcg cgtccggaat ggcagacgtt gacgactttg atttatgcct atttggggcg
     3661 tgcgggtgag gataattatg cgcgggcaat ggtgttgaca ttgctgttgt cggcatttgc
     3721 ggtgtgcatt ttcctgctgt tggacggcgg cgaaggcgga aaacggacgg aaacgttata
     3781 atgcgaaccc tttttcagag gacggcaaaa tgagcgagtt ggataacatc cttgcccata
     3841 accggcagtt tgtcgagtcg ggcgaatatg aaaaatactt taccgacaaa taccccgaac
     3901 gcgggctggc ggttttgtcc tgtatggatg cgcggattat cgggctgctg cccgacgcgt
     3961 tgggtttgaa aaacggcgat gccaagctga ttaaaaatgc cggcgcgctg gttacgcacc
     4021 cgtggggttc ggtgatgcgg agccttttgg ttgccgtgtt tgaactgaag gtcagagaga
     4081 ttatggtcat cgcccatcac gattgcggta tgcaggggct gaatgccgaa gaattcctcg
     4141 ggcgcgtccg ggaaagccag attcccgaag accgtatcga aaccctgcgt tatgccggta
     4201 tcgacctcga cggctggctg accggtttcg acaacgtcga agacagcgtg cgccacacgg
     4261 tggaccttat ccgtaaccat ccgctgatgc cgcgccatat cgccgttcac ggactggtca
     4321 tccatcccgt taccggcaaa ctgacgctgg ttgtggacgg cagtgtttca gacggcatgg
     4381 acttatcgga aggaatggaa acatcatgaa gaaaatcgga ctgttcggcg gcactttcga
     4441 cccgatacac aacgggcatc tccacatcgc ccgcgccttt gccgacgaaa tcggcttgga
     4501 cgcggttgtt ttcctgccgg caggcggccc gtatcacaaa gacgcagcct ccgcttccgc
     4561 cgccgaccgc cttgccatgg tcgaactggc gacggcagaa gacgcgcgtt ttgccgtcag
     4621 cgattgcgac atcgtccgag aaggtgcaac ctatactttt gataccgtcc aaatcttccg
     4681 ccagcagttc ccatccgcgc aactctggtg gctgatgggc agcgacagcc tgatgaagct
     4741 gcacacatgg aaaaaatggc agatgctcgt gcgcgaaacc aatatcgccg tcgccatgcg
     4801 gcagggcgac agcctgcacc aaaccccgcg cgaactgcac gcgtggctgg gcaagtccct
     4861 tcaggacggc agcgtccgca tcttgtccgc cccgatgcat aatgtgtcgt caacggaaat
     4921 ccgccgcgcc ggtgtttcag acggcatccc acctgccgcc gcacgctaca tccgcgaaca
     4981 cggtttgtat gaaaaataaa gtcaaatcag taagaaacgg ctataatgcc gtctgaaaac
     5041 atcccgttag gaaaataatg aacgaacaag aactgcaaga cctgcaaaaa atggtcgggg
     5101 tcgccgtcaa cgccctcgaa gacatcaaag ccaaagacat ttccgttctc gaaacgcaag
     5161 acaaaacttc gctgtttgcc agaatgatta tcgccagcgg cgacagtacg cgccaagtca
     5221 aagcactggc caacaacgtt gccgtcgatt tgaaagaagc cggttttgaa atcctcagta
     5281 ccgaaggaga cagcggcgaa tggacgctcg ttgatgcagg agacctcgtc gtccacgtta
     5341 tgctccccgc cgtgcgcgac ttctatgata tcgacaccct ttggggcggc gagaaaccga
     5401 gttttcacgc cggaatgcag aagccgtggc acgcggcaga ctgattcccg atgccgtctg
     5461 aatgttcaga cggcattttc ctgtaaggga gaaagcattg aacattaccg ttttggcagt
     5521 cggcaccaaa atgccgcgct gggttgatga ggccgtcgcc gaatacgcca aacgcttcgg
     5581 acgcgacgtc gcctacgcac tcaaagaaat caaacccgaa aaacgcggcg cgggcgtgaa
     5641 tgccgcccaa ggtatggcgg cggaagaaaa acgcatcctc gaagccgttc cgcaaggcgc
     5701 gttcctcgtc gttcttgacg aacgcggcaa agcgccgacc tccgtcgagc tggcggaaca
     5761 cctcaaaagc tggcggcaaa acggcgaaca cgtctgcttc gtcatcggcg gcgcggacgg
     5821 catgaccgac cgcctcaaac aacaagcccg catgatgatg cgcctgtcca gcctcaccct
     5881 gccgcacggc atggtgcgcg tctttctgac cgagcagctc taccgcgccg tttccatcct
     5941 gcacaaccat ccctatcatc gggaataaga aggcttttgt ccgtatccct aatcggttaa
     6001 aatcaccccg ttatttctga acacagcaaa ggaatccgct atggcacgca tggtattctg
     6061 cgtcaagctc aacaaagaag ccgaaggcat gaaatttccg ccgcttccca acgaattggg
     6121 caagcgcatt tttgaaaacg tatcgcaaga agcgtgggcg gcgtggacgc gccaccaaac
     6181 gatgctgatt aacgaaaacc gtttaagcct cgccgatccg cgcgcgcgcg aatacctggc
     6241 tcagcagatg gagcagtatt tcttcggcga cggcgcggat gccgttcagg gatacgttcc
     6301 gcaataacgg ttttccgttt tgaacacagg ctgtccgaaa ctgcttcaga cggcctttaa
     6361 aatacgccgg acaactttat cttcacgcaa ccggacaaat tttgacattg ggcaatgtta
     6421 aaatccccca ttattcccaa ccgcgctttc aggagcagat gatatgcaac acgacgttta
     6481 cgactacacc gcgcatacgg tttctaaaaa caccgtcctg cagaaaacct accgcctgct
     6541 cggattttca ttcattccgg ctgccgcagg cgcggcactt gccgccaatg ccggtttcaa
     6601 tttttacgcc gccttcggtt cgcgctggat agggtttgcc gtcgtgttgg cgtttttcta
     6661 cggtatgatc cacttcatcg agaaaaaccg ttacagcaat accggcgttg ccctgctgat
     6721 ggtattcaca ttcggtatgg gcgtattgat cggccccgtg ctgcaatacg cgctccatat
     6781 tgccgacggt gcgaaaatcg tcggcattgc cgccgcgatg accgccgccg tctttttaac
     6841 gatgtccgct ttggcgcgcc gaacccggct cgatatgaac gcgctcggac gcttcctgac
     6901 cgtaggtgcg gtcattctga tggtcgccgt ggttgccaat ctgtttttgg gtattcccgc
     6961 actcgccctg accatttccg ccggttttgt cttgttcagt tccttaatga ttatgtggca
     7021 ggtacgcacc gtcatcgacg gcggcgaaga cagccacatc agcgcggcac tgacactgtt
     7081 tatctcgctt tacaacatct tcagcagcct gctcaacatc ctgttgtctt taaacggcga
     7141 ggattgatgc cggtaaaacg ccgtccgact atgccgtctg aaaatgcttc agacggcatt
     7201 ttactttggg catacaatac ggatgcacgc acatatagtg gattaacaaa aatcaggaca
     7261 aggcgacgaa gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ctgtactggt
     7321 ttaaatttaa tccactataa aaacacagcc ggctatgaaa ccgggaggaa tcaaatgtta
     7381 ccgaatacac cgcgccgcgc cgtttatgcc ggcagtttcg atccgcccac attggggcat
     7441 ctgtggatga tacggcaggc gcaatctatg tttgacgaac tcatcgtcgc catcggcatt
     7501 aaccccgaca aacgcagcac ctataccgtc gctgaaaggc aggatatgtt gtgcgatatt
     7561 actaaaatgt ttcccaacgt cagaaccgat gtatttgaaa accgatttct ggtgcattac
     7621 gcccgtgagg tagatgcagg attcatcgtg cgcggcatcc gttctgcttc ggattacgaa
     7681 tacgaacgtt ccatgcgcca tatcaacagc gacctcgccc ccgaaatatc caccgtattc
     7741 ctcatgccgc cgcgcgaaat cgccgaagtg tcgtccacta tggtcaaagg actggtcggg
     7801 ccggaaggct ggacggaaac gatccaccgc tacgtgccgc aagctgtgta cgaaaaaatc
     7861 cttgccgaac atcaacacga aaattgaacc cttctatttt tgcaaggcat atatggatat
     7921 gttggaaatt ttgttccgcc atcaagactt cgtcgccatc aacaaaccgg gcggcatatc
     7981 cgtccaccaa gacagcggcg agaccggact ggcccgaaca ctcgccatac agttgggcgt
     8041 agagcgtgtg tggctgctgc accgattgga caaacagacc agcggcattt tactgtttgc
     8101 cctcaacaaa gaaagcgcgt ccgcccttag cggacagttt gccggcaaaa gcatcaaaaa
     8161 aacctatttg gcactgtccg accgcaagcc gtccaaaaag cagggttgga ttaaaggcgg
     8221 aatggaaaaa tccagatgtg gaatgtggaa gttgacgcgc aatacagaaa atatcgccgt
     8281 tacccgattc cacagcatca gtatcgccga aaaactgcgg ctgttcatcc tagaaccgca
     8341 tacgggcaaa acgcaccaat tgagagtggc gatgaaaagt ttgggcagtc cgattctggg
     8401 cgacagctta tacggcggaa cagaatccga aaccatgttt ctgtatgcat ggaaaataca
     8461 atttgactac caaaaccgac aaatcgaaat tgttgcaccc ttaaaaaacg aatggcagac
     8521 tgaaaatatc catcgggcac tggaagaatt ctgtatggaa aataaaattg actgatataa
     8581 attaaattta taaacaaata gataaatatt tttaaaaaaa tttcttcag
//
LOCUS       DAWHRS010000085         8479 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWHRS010000085 DAWHRS010000000
VERSION     DAWHRS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.085"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..290)
                     /gene="trxA"
                     /locus_tag="WIB82_001373"
     CDS             complement(<1..290)
                     /gene="trxA"
                     /locus_tag="WIB82_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ3842664.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALA"
     gene            588..1061
                     /locus_tag="WIB82_001374"
     CDS             588..1061
                     /locus_tag="WIB82_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ3842665.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGATMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYVLTKEMVDDLNDHIHLENDILFARVLDS"
     gene            complement(1132..1953)
                     /gene="nadE"
                     /locus_tag="WIB82_001375"
     CDS             complement(1132..1953)
                     /gene="nadE"
                     /locus_tag="WIB82_001375"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ3842666.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGTHQTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            2150..3505
                     /locus_tag="WIB82_001376"
     CDS             2150..3505
                     /locus_tag="WIB82_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ3842667.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAVRLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIESCTVPVVSGVGHETDFTLADFVADMRAPTPTGAAELVSPNRQESLHRLAQ
                     AQCRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNVRT
                     HTARFERQTQALKHCRPDVSVCKKNIDRFQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            3798..5339
                     /locus_tag="WIB82_001377"
     CDS             3798..5339
                     /locus_tag="WIB82_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ3842668.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(5408..6457)
                     /locus_tag="WIB82_001378"
     CDS             complement(5408..6457)
                     /locus_tag="WIB82_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ3842669.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKPQNVPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(6466..6693)
                     /locus_tag="WIB82_001379"
     CDS             complement(6466..6693)
                     /locus_tag="WIB82_001379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842670.1"
                     /translation="MQIHLLLLLSFERFYSRTQTDFNRRTNFAPTPSRSDDFIRKTAA
                     SGTISNRPTEDGILSTRPAAHAAEEPPYQAS"
     gene            6682..7314
                     /gene="pdxH"
                     /locus_tag="WIB82_001380"
     CDS             6682..7314
                     /gene="pdxH"
                     /locus_tag="WIB82_001380"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ3842671.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(7444..8454)
                     /locus_tag="WIB82_001381"
     CDS             complement(7444..8454)
                     /locus_tag="WIB82_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ3842672.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLTAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCCTTAQSDININIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANLPDQEGILE
                     LHIRRHENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSNRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRNGHVQDIAAQDHPDLSEYEVFACGSPAMTEQTKNLFVQQHKLPENLFFSDAFTPS
                     AS"
BASE COUNT         1761 a         2190 c         2469 g         2059 t
ORIGIN      
        1 gccaatgcgc cgactttggt ggcgacgact tcgccgtttt tgaacaccat cagggtcgga
       61 atgccgcgca cgccgaaacg ggacggggtg gcttcgttgt cgtcgatgtt gattttgacc
      121 actttcagac ggccttcaaa ttcggcggca atgtcgtcca aaatcggggc aatcattttg
      181 caggggccgc accacggagc ccaaaagtcc agcaggacgg ggatatctgc atttaaaacg
      241 tctttttcaa aagcggcatc gctggtgtgt acaatcaatt cgctgctcat tatttttcct
      301 tttcggttgg gattggcacg atagcgtgca ggatagggct tgggcataaa aatttcaagt
      361 gggcgggcgg gataatagtt tttctgtacc gccccaatcg cctttgcaaa ttctaaaaaa
      421 cggttcggac agtattttat ttctgaaaat tacttttatt atcaaaatgt aaggaatacg
      481 tttgaggctg tacagaagcg cgcgggcggt gtatgctccg tttgaattga ttcttaaagt
      541 atgaatgttc aaaaccaaac ccgaaaccct gtaaaaggaa cactgcgatg actgattttt
      601 ccgtttggga gaccgccccc tttggtgcaa ccgtcgatca tatcctgcaa cgctaccaca
      661 atgtccaccg tgcacaattt gaagaattgg tgccgctggc gcaaaaagtg gcccaagttc
      721 atgccgacac ctttccggcg gaaattgccg agctgcttgc ctatatgcag aacgaactgc
      781 tgatgcatat gatgaaagag gaaaggatgc tgtttccgat gattaatcag ggtgtcggcc
      841 ggggtgcgac gatgcccatc agcgtgatga tgcacgaaca tgaagaacac gaccgcgcca
      901 tcgcacggct caaagagctg accgataatt tccaagcgcc cgaaggagcc tgcggaagct
      961 ggacacggct ttacgttttg accaaagaaa tggtggacga cttgaacgac cacattcatt
     1021 tggaaaacga tattctgttt gcccgcgtac tcgattctta aggaacggga gccggatatt
     1081 tccatgccgt ctgaaatacg gtttttccgc ttcagacggc atttccacgt ttcagcccaa
     1141 caattcgggc ggaatgcggc acacgggaat cgggttgatt ttgtgctgca tcgcgcggtg
     1201 cagccgcgta tagatttcca gaacttcgcg ctgccgccct tcaaaatctt cgggtttgcg
     1261 cgtgccgtac acgcccattg cccactccag ttcgggatag cttgcgccca tctgttcttc
     1321 gtcggtgcgt tccgtatccc acaggccgtc ggtcggcggg gctttttgaa tcgcctcgtc
     1381 cacgcccaat gcttcggcaa gccggtaaac ctgcgttttg gtcaggtcgg caatcgggct
     1441 gatgtccacg ccgccgtcgc cgtatttagt aaaaaagccc acgccgaaat cttcaatctt
     1501 attacctgtc cccgtaacca gcagtccgtg tatctgcccg tagtagtaca gggtcagcat
     1561 acgcaggcgg cttctggcgt tggcgaggga aagcggctga ttggcaaatg ccgtctgatg
     1621 agtgccgacg gtttgttcaa aggtctggaa ggtgtcggtc agatcgaccg tttgcgcgct
     1681 tacattggca tattgccgtt gcagattgcg gatgtgcagc cttgcccgct caagctggcc
     1741 ggggtgttgg cgtatcggca tatccagaag cagcgtgggg cggccggtgc gggcggcgag
     1801 tgcggagacg acggcggaat cgatgccgcc ggaaacgccc acgacaaacc cttttgcatt
     1861 tgcccgggcg gcgtattcgt ccaaccatcg ggcgatatgt gtgataactg cctgcgtatc
     1921 catttttcct cctgtcgaaa atatccgcat catagcctgc cggcggtgca atttgaatag
     1981 attgcaggca ggttgatgaa tatttttccc attccccgct taaaaaagtc atacgggaaa
     2041 aggcgggcgg caagatggcg cgtgttataa tattgttgac aatttaggcg gcccggtgcc
     2101 gccttaggct tcaaaacata ttcatatttt ttactatttg attgattcga tgtctgattt
     2161 ttttcattct gacgtgttgt ccgtttccga gttgaacgca tttgccaaaa gtcttttgga
     2221 aaaccacctt gccggtttgt ggattgccgg cgaagtatcc aacctgaccc gtgccgccag
     2281 cgggcattat tatttctcgc tcaaagacag ccgcgcacag gtgcgttgcg cgatgttcaa
     2341 gggtgcggcg gtgcgtttgg cgaagccttt gaaagaaggc gaccatatcg aggtatcagg
     2401 aaaaatcagt atttatgaag cgcggggcga atttcagatt accgtaaacg aagtacggct
     2461 caaaggtttg gggcagcttt acgaagccta tgagcggttg aaggcgcagt tgcaggcgga
     2521 aggcgcgttt gcggcggaac gcaagaaacc tttgcccgtc cgtccgcaat gtatcggcat
     2581 cgtaaccagt ctggcggcgg cggctttgcg cgatgtcgtt actaccttga aacgccgcgc
     2641 acccgaaatc cccgttatcg tttatccgac acccgttcaa ggcgcaggca gcgaattgca
     2701 aattgcccaa gcgattaaaa ccgcctcgca acgcgccgaa tgcgatgtgt tgattgtctg
     2761 tcgcggcggc ggcagcattg aagacttgtg ggcgtttaac gaagaaccgg tcgtgcgcgc
     2821 catcgaatcc tgcacggttc cggtcgtcag cggcgtaggc cacgaaaccg atttcacgct
     2881 cgccgatttc gtcgccgata tgcgcgcgcc cacgccgacc ggtgcggcgg aactggtcag
     2941 ccccaaccgc caagaatcgc tgcaccgcct cgcccaagcc caatgccgtc tgaaaaccgt
     3001 tttggaacaa cgctatttcg atgccagcca aaaactcgac tggctggcgc ggcaaatccg
     3061 tcatccgcgc caaaaactcg acgaacagcg cgcttcaatc ggcaagctgg cgcaaacgct
     3121 gtcttactcg atgacgcaaa acgtccgtac ccacaccgcc cgtttcgaac gccaaaccca
     3181 agccctgaaa cattgccgtc ctgatgtttc cgtttgcaaa aaaaacatcg accgctttca
     3241 gacggccctg tcgcacgcct tccaccaact gcttacccac cgccgccaaa gcctgactgc
     3301 ccaagccgca ttgctcgaag ccgtttcgcc gcagcacatt ctggagcgcg gcttttccgt
     3361 cgtcaaaaac acacgcggac aagtcatccg caacgccgat gttttgaagc aggggcagaa
     3421 gctgcacatt atcttcgccg acggcgaaac cgacgtgcgc gtaagcaaag aacaagggca
     3481 gcaggatttg ttcgattgca tttaaaccca atcggaaaaa ccgctccccc gccgccgtca
     3541 ttcccgcgaa agcgggaatc cagtcccttg accttaagat aatctttaat tcgtcattcc
     3601 tgaaaaagcg ggaatccgga agcgaaaagc cgcaggaatt tgtcggaaaa aacagaaacc
     3661 tttccgtaca cccgaaatgt ttcagacggc gcatcttggg gcggataaaa acccgacccg
     3721 atattttttt ataattttca ataagtttcc attgggcaaa tgaaatttta tttttcaact
     3781 gacagaaaga ggatgttatg aagtttttag accgtgaggc aagcattgcc aagccgggtt
     3841 tcaaccgttg gctcgtaccg cccgcagcat tggcggtaca ccttgccatc gggcagattt
     3901 acgcctattc cgtattcaac gcgccgctga ccaaacttat cggcataacc gaatcggcgg
     3961 ccggagattg gaagctgacg accgtaggtt ggattttcag tatcgcactc gcgatgctgg
     4021 gcgcgtcggc cgccctgttc ggtacgtgga tggaacgggt agggccgcgc aaagccatat
     4081 ttgccgccgc ctgctgcttc agcctgggct tcttcgtatc cgcattcggc gtgcgtacgc
     4141 acaacctctt cctgctctat ttgggcaacg gcgtcatcgg cggcgtaggc ttgggtttgg
     4201 gctacatcgg gccggtttcc acactgatga aatggtttcc cgacaagccg ggtatggcga
     4261 ccggtttggc gattatgggt ttcggtggcg gcgcaatgct ggcctcgccg ctttccgtat
     4321 cactgatgaa caccttttca aacgctgctt cggtcggggt tgccgaaacc ttcgccgtat
     4381 tgggtctgct ttacctcgca ctgatgatgt tcggcgcgtt taccatccgc gtacctgccg
     4441 acggctggaa acctgaaggc tataccgcgc cgaaaacgca aaacaagctg gtcagcagca
     4501 atcatgtcaa cgtgtcccaa gccatgaaaa cgccgcagtt ttggctgttg ttctgggtat
     4561 tgtgcctgaa cgtaactgcc ggcatcggcg tattgggtca ggcatccgtg atgattcagg
     4621 aactcttttc cgaaacctcc ataggcaggc aggcagcagt cggcgcaggt gcggcggcag
     4681 gcttcgtcag cctcctgagc ctgtttaaca tgggcggacg ctttttgtgg tcgagcgttt
     4741 ccgacaaaat cggacgcaaa aacacctaca ccatcttctt cgtactcggt tcgctgctgt
     4801 atttcgccgt tccctccatc ggcgagggcg gcagcaaagc cctgttcatc atcggctttt
     4861 gcgtgattat ttccatgtac ggcggcggat ttgccgccat tcccgcctac ctgaaagacc
     4921 tgttcggcac ctatcaggtc ggcgcgattc acggacgcat cctgctggcc tggtcaaccg
     4981 ccgccgtcat cggcccggta ctggtcaatt acatacgcca aagccaaatc gacagcggca
     5041 taccggcggc acaggcatac agcgtaacca tgtacatcat ggcgtgcctg ctgattatcg
     5101 gtttgttgtg caacctcgcc gtcaaatccg tacacgaaaa acaccacgaa aaagacatca
     5161 aaaccgccgc acacagcggc aaccccgatg acgaaaccgc catatccgac gcttaccttg
     5221 tgggcgaaaa agtttccggc ggcggcatat cggtctggtg gcgttgggca ttggccgtca
     5281 taccgctcgc ctacggcgtg gtgatggtct ttgtcaaagc actcgacctt ttctcctgaa
     5341 aacgcccgaa taaaatgccg tctgaaacag cagatgtttc agacggcatt tttgtgtttt
     5401 ggcgggttta ggcttttttg cggcggcgtt cgcccggcag cagcatgtcc gcccatttga
     5461 tgatgttggc gacttcggcg gggttgagtt cgtagaactg cccgcgtttg aggcggttgg
     5521 gcagtccgat gggaccgaag ccgatgcgca cgaggcggct gacggtgagt ccttggcttt
     5581 caaaaatgcg gcgcacttcg cggttgcggc cttctttaat cacgacgttg taccatttgt
     5641 tcgcgccttc gccgccttgt tcgcggatgc gttcgacttt tgccaagccg tcttcgagca
     5701 tcacgccttc ttcggtgagg acgcgcattt gttcgccggt cagcccgccc aagacgcgca
     5761 cggcgtattc gcgttcgact tcgaagctgg ggtgggcgaa acgttggacg agttcgccgg
     5821 aggtggtaag aatcagaagt ccgctggtgt tgatgtccaa gcgtccgatg gcgacccagc
     5881 ggctgctggc ggcctgcggc aggcggtcga atatgctgac gcggccttgc gggtcgtcac
     5941 gggaaacgat ttcgccttct tgtttgtaat acaggatgat gcgcggcagg cggtccgccc
     6001 atttgagctt gatgatgctg cctttgacgg taacgtggtc gtcgggggta actttgtcgc
     6061 ccagttgcgc ggttttgccg ttgaccgtta tccagccgtt ggtaatccat tcttccattt
     6121 cgcggcgcga gccgacgccg gacgcggcaa gcactttttg caggcgttcg ggttccatgc
     6181 gcgacaggtc gctgcggcgt tctttcaaat cgcgcgcgtg ttccataatt ttttggttgg
     6241 gattgcggac gacgagtttt ttggctttgg cggcgcgctg tttggggacg ttttgaggtt
     6301 tgatgccgtc tgaagctttt tgtccgtaag gttgggaagc ggttttgccc gtttcatctt
     6361 tgggacgggc tttgcttttg aacggtttgg cggttttctt ggcagacggg gctgcgccgt
     6421 cgcgccattg gcgtttgctg gtgggctgtt tggacatcat ttttcctaac tcgcctgata
     6481 aggcggttct tctgcggcgt gtgcggcagg acgggttgat aaaatgccgt cctcggtcgg
     6541 acggttcgat attgtacctg atgcggcggt tttgcggatg aaatcgtcgc tgcggcttgg
     6601 ggtcggggca aagtttgtgc gacggttgaa atcggtttgc gtgcgggagt agaatctttc
     6661 aaatgataag aggagcagta aatggatttg cacaatattc gggaagatta cagcaaacgg
     6721 gaattgtcgg aagccgattg cgccgataat ccgatcgagc agttcgagcg gtggttggac
     6781 gaggcggtac gcgcacaggt caacgagccg acggcggtca atgtggcggc ggttgacgga
     6841 cgcggcaggc ccaacagccg tatggtgctg ctgaaggaag ttaattctga aggttttgtt
     6901 ttctttacga attatcacag ccgcaagggg cgttcgctgg atgctcatcc atttgcggcg
     6961 atgacgtttt tttggccgga gctggagcgt caggtgcgcg tggaagggcg tgttgaaagg
     7021 ctggcggaga agttgtcgga cgaatatttt gagagtcgcc cgtatcagag ccgtttgggt
     7081 gcgtgggcaa gtgcgcagag cgaggttatt ccgaacaagg cggtattggt ggcaaaggcg
     7141 gcggcggttg gactcaaaca tcctctgcat gtgccgcgtc cgccgcattg gggcggctat
     7201 attgtgattc ccgatttgct tgagttctgg cagggcagac cgagcaggct gcacgaccgc
     7261 atccaatacc gtttgctgga tggtggctgg atacgcgagc ggctgtcgcc ttgaacagga
     7321 aaaaacatat gccgtctgaa aaccggaatc gggtttcaga cggcattttt tcacggggaa
     7381 aacacggacg ggaagttttt tgtgttctga acggaaagct cgaatcctct ttataccggg
     7441 gaattatgat gcggacggcg tgaatgcgtc ggagaaaaac aagttttccg gcagcttatg
     7501 ctgttgcaca aacagattct ttgtttgttc ggtcatggcc ggagaaccgc aggcaaatac
     7561 ttcgtattcc gacaggtcgg ggtggtcttg tgccgcgatg tcttgtacgt gaccatttct
     7621 tccctgccag ccctctccgg ggcgggacaa tacgggggtg aagcaggcgt ttttcagacg
     7681 gcatgccaac ccttgtgctt cttcgagggc atacaaatca tcctgatgac gcgcgcccca
     7741 gtagaaatgg acggcgcggt tgctgccttg gcggataagg tcgagcagga tgctgcggat
     7801 gggggcgtag cctgtgccgg ttgccagcag gatgacgggt ttgccgctgt cttcctgcaa
     7861 ggtaaacgaa ccgagcgggc ctttaacgcg gacgatgcct ttttctttga ctttgggttc
     7921 gctgccgaaa atcatttccg agcagacacc gttttcgtgc ctgcggatgt gcagttccaa
     7981 aatgccttct tggtcgggta aattggcgat ggagtagctg cggctgacgt tgcccggcag
     8041 cagtaaatca atgtattgcc cggcgtagaa ggcaaacggc ggggctttgg gcagggcaag
     8101 tttcaggagg gcgacatcgt gtttgaaaat aatactttcg atgcgtgcgg gcagggtgcg
     8161 gacgggtagg gcatcggctt tgtagccggg gatgttgatg ttgatatcgc tttgcgcagt
     8221 ggtgcagcac atcaaaatct tgccttgcgc tttttctgct tcggataaag cctgttccga
     8281 gtgtccgccc atttgaatat cgccgctgac cagttcggct ttgcattgtc cgcagacacc
     8341 gcttttgcag gaatggggca ggttgaggtt ttgacgggcg gcagcggtca aaacggtttc
     8401 gccgtcgttg gcggcaaagg tggtttggtc gggcagggta acggtgtggt tcatgtcttg
     8461 gtatggtttg agcagatta
//
LOCUS       DAWHRS010000086         8385 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWHRS010000086 DAWHRS010000000
VERSION     DAWHRS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.086"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..332)
                     /locus_tag="WIB82_001382"
     CDS             complement(<1..332)
                     /locus_tag="WIB82_001382"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ3842673.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSR"
     gene            complement(351..1220)
                     /gene="galU"
                     /locus_tag="WIB82_001383"
     CDS             complement(351..1220)
                     /gene="galU"
                     /locus_tag="WIB82_001383"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ3842674.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(1248..1847)
                     /gene="rdgB"
                     /locus_tag="WIB82_001384"
     CDS             complement(1248..1847)
                     /gene="rdgB"
                     /locus_tag="WIB82_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ3842675.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDTEVKNRESHRAQALAELLRKLAL"
     gene            complement(1840..2073)
                     /locus_tag="WIB82_001385"
     CDS             complement(1840..2073)
                     /locus_tag="WIB82_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842676.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLHAAQSATKENRYV"
     gene            complement(2181..2714)
                     /locus_tag="WIB82_001386"
     CDS             complement(2181..2714)
                     /locus_tag="WIB82_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ3842677.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDVE
                     EAKKVIKESIERWNKQA"
     gene            complement(2816..3274)
                     /gene="nudB"
                     /locus_tag="WIB82_001387"
     CDS             complement(2816..3274)
                     /gene="nudB"
                     /locus_tag="WIB82_001387"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ3842678.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            3485..3561
                     /locus_tag="WIB82_001388"
     tRNA            3485..3561
                     /locus_tag="WIB82_001388"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3519..3521,aa:Pro,seq:ggg)
     gene            3720..5612
                     /gene="mafB"
                     /locus_tag="WIB82_001389"
     CDS             3720..5612
                     /gene="mafB"
                     /locus_tag="WIB82_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ3842679.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHTQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEMKKGRGG
                     KPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKWRGTSKSGVEIEGFTEPNRTAY
                     PLYNSK"
     gene            5639..6058
                     /locus_tag="WIB82_001390"
     CDS             5639..6058
                     /locus_tag="WIB82_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842680.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            6136..6219
                     /locus_tag="WIB82_001391"
                     /pseudo
     CDS             6136..6219
                     /locus_tag="WIB82_001391"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6216..6458
                     /locus_tag="WIB82_001392"
     CDS             6216..6458
                     /locus_tag="WIB82_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ3842681.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            6536..6781
                     /locus_tag="WIB82_001393"
     CDS             6536..6781
                     /locus_tag="WIB82_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ3842682.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMDKGK"
     gene            6784..7170
                     /locus_tag="WIB82_001394"
     CDS             6784..7170
                     /locus_tag="WIB82_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842683.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPVSKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
     gene            7272..7661
                     /locus_tag="WIB82_001395"
     CDS             7272..7661
                     /locus_tag="WIB82_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842684.1"
                     /translation="MWKIIKEDSDDLEFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIYVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            7688..7837
                     /locus_tag="WIB82_001396"
     CDS             7688..7837
                     /locus_tag="WIB82_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ3842685.1"
                     /translation="MRLSDGIFDIKRITKRAFDSKGTSEIPDYTWHHHQDTGCIGWKA
                     MNKRR"
     gene            7840..8226
                     /locus_tag="WIB82_001397"
     CDS             7840..8226
                     /locus_tag="WIB82_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842686.1"
                     /translation="MWKIYKENSTDLNFSLGSIYCQAINITEFKMWVEKIIREMDLDE
                     IPNYFFDLTDLQSLFHLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDSPISKEKA
                     LKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         2236 a         1785 c         2156 g         2208 t
ORIGIN      
        1 ttgcggctgc ggccggtgtg caggcgtttg cccgcgtcgc cgattttgtc ggtcaggcgg
       61 cgttcgatgt tcatatggac atcttccaaa tcggacgacc attcgatttt gccgctgcgg
      121 atttcttcga ggatttccgc cataccccgg cggatgtccg ccaaatcgcc ttcgtccaac
      181 acgccggttt cttttagcat ttgcgcgtgt gccagcgagc cttggatgtc ccattcggca
      241 agccgtcggt cgaaaccgat ggaggcggtg tattgtttga cgagttcgga aacgggttcg
      301 ttgaaacgtc cggaccaggt tttgtcgtgc ataaggattc cttgatgggg ttattcggtg
      361 cggtattttt ccaaaagccg gcggaagggt tcgccggttt cgggatgttt cagaccgtag
      421 gcgacggtgg cttcgaggta gcccagtttg ctgccgcagt cgtagcgcgt accttcaaag
      481 gggtgcgcca ggacaaattc gtgatcgagc agcttggcga tgccgtctgt aagctggatt
      541 tcgttgcccg cgccgcgcgg aagattggtt aagaggtcga aaatgcgcgg ggtgaggatg
      601 tagcgtccaa caacggcaag gttggagggc gcgtcttcgg gcttgggttt ttcgacaatg
      661 ccggtaatgc gttggaactg tttgagctgt tcggtttcga cgatgccgta tgagccggtt
      721 tgcgatgctt caacggtttc tacgcccaaa atgctgttgc cgctgcgccc gtacacttcg
      781 accatttgtt tgagcgcgcc tttgggggca tcaatcaggt cgtcggcaag gataacggca
      841 aagggttcgt ctccgatggc ggcgcgggcg cacaagacgg cgtgtcccaa gcccagtgct
      901 tccgcctgac ggatgtagag gcaggtaatg ttcggcggca ggatgttgcg gacgtgttcc
      961 aacaatttgt ctttatggcg catttccaac tcggtttcga gttcgtatgc cttgtcgaaa
     1021 tggtcttcga tgctgcgttt gttgcgtccg gtaacaaaca ccatttccgt gcagccggct
     1081 tccacggctt cttctacggc gtattggatc agcggcttgt cgacgatggg cagcatttct
     1141 ttcgggctgg ccttggtggc gggcaggaag cgggttccca tccctgcgac ggggaaaacg
     1201 gctttccgta tgggtttcat tctttttcct ttgtattgtt ttgatgttta aagggcgagt
     1261 ttgcgtaaga gttcggcaag tgcctgcgcg cggtggcttt cgcggttttt gacctccgta
     1321 tccaattcgg cggcggtttt gccgtgttcg ggcagataaa aatacgggtc gtaaccgaaa
     1381 ccgttttgcc cgagcggcgt gtcgttccac tgcccgtgcc atacgccctc ggcgataatc
     1441 gggcgcgggt cgtctttatg gcggacaaaa accaatacgc agacatagca gcagcttttg
     1501 tctgccttgc cgacaagttc ggcggcaagt ttcaggttgt tggcggtatc ggatttggga
     1561 ttgtcgcccg cgtaacgtgc ggaatggatg cccggcgcgc cgtttaaggc ggcggcacag
     1621 atgccgctgt cgtcggcgag tgcgggcagc ccgctgtatt tggcggcatg gcgtgctttt
     1681 gccagcgcgt tttcgacaaa ggtgggatag ggttcggggc attcgggtat gccgaatgcg
     1741 gattgcggca atacggtgat gctgtaaggt ttgaataagt tgccgaactc ttcgagcttg
     1801 ccggcattgc cgcttgccaa aacgattttt tccggttttt cagacatagc ggttttcctt
     1861 tgtggcggat tgggcggcgt gtagggattt gtgccgcaaa tagagggcga gtgcgccgat
     1921 ttgcccgaac agcgcgccga atgcgaaaag aaaggaggcg acggcgaagg ctttgctgcc
     1981 gacggtcagt agataagcac ccaagaggac gagcagcata aatgccagtg tgaacagggc
     2041 aacagacaac agatagattt ttcggcggtt catggcgttc ggtcggaaac ggtatgttcg
     2101 gattatatcc gattgggacg gcattcccta gagcttggaa aaatgccgtc tgaaggggcg
     2161 ttcagacggc atggcgggcg ttatgcctgt ttgttccagc gttcgatgga ttctttgatg
     2221 acttttttcg cttcttccac atcgccccag gattcgactt tggtcgtacc tgcttttttc
     2281 aggtctttgt aatggttgaa gtggaactcg atttgtttga tgagctgttg cggcaaatcg
     2341 gacaaagttt tgtaggcgtt gccgttgttg cggtcgtcgg caggaacgca gacgattttg
     2401 tcgtccactt cgccgtcgtc aacgaatttc atcacgccga taacgcgcgc ttccaagaat
     2461 acgccggttg ccagaggctg ttcggtaacg agcagcacgt ccaattcgtc gccgtcttcg
     2521 tccaaagttt ggggaatgaa gccgtagttg gtcggtttgg cgaagatggc gggttcgacg
     2581 cggtcgagtt ggaatgcggc gagtttgcgg ttccactcga ttttgtggtt gctgccggcg
     2641 gggatttcgt tgacgacgtt gatgatgccg ccgtccacat cgccgggggt caggatttgg
     2701 ttgaagtctg ccatttggtt tcctttgttt gggaaagttt gaagtttgaa agtatagcac
     2761 aaacgtccgg ctgaaaatgc gcccgatgcc gtctgaaagg gtgtacgggc gcgtgttacc
     2821 gtttgcccaa aaacctgccc agttccaaaa ttgcgcgcct gttggacggg gaaaacactt
     2881 tttccgccgc ttcttccaag ccgaaccagc cgtaggagac gtgttcctcg ggttgcagga
     2941 cgacgggcgt atcgcgcggg atttcggcag agaagaggtg ttcgcggttt tcaaacacgc
     3001 cttttggata gcggtgccgc cagtggtggt agatttcgta aaccgtgctg tcgtgccagt
     3061 cttgaagctg cccgtccgcc agcaggatgc cggtttcttc ccaaacttcg cgccttgccg
     3121 tttgggcgac ggtttcgcct ggttcgaggc tgccggttac cgactgccaa aatcctttcg
     3181 gatgcgtgcg ttcgatgagc aggatgccgc cgtccccgct ataaaggacg accagtgcgg
     3241 aaacggggta tttgagcggt tttgccatcg gcatctttcg gcgggctgcg gtaatgaagg
     3301 ggcggattat agcaaacgcc gcacgttatg gcgtttatcc ttttccgtat ccttttttct
     3361 gcacggatgg gacgcgccgg tgtttgccgg taaattttcc gttgtgtcaa aaagataagg
     3421 gcggttgtga ttttaatgct tgccaaagcg tcgggcggaa actataatcc gaaacttatc
     3481 gagtcggagt gtggcgcagt ctggtagcgc acttgcatgg ggtgcaaggg gtcgaaggtt
     3541 cgaatccttt cactccgacc aaaaattccg aaagccgctt tcaaaagcgg cttttttgcc
     3601 gtccgtatga ttatgatgta gagtacgcgg cgacagacat tcaaatgccg tctgacaaac
     3661 cgttcagatg gcatctcttt atcttagttt cattccgtac catcttaagg aacatcaaat
     3721 tgggcatttc ccgcaaaata tcccttattc tgtccatact ggcagtgtgc ctgccgatgc
     3781 atgcacacgc ctcagatttg gcaaacgatt cttttatccg gcaggttctc gaccgtcagc
     3841 atttcgaacc cgacgggaaa taccacctat tcggcagcag gggggaactt gccgagcgca
     3901 gcggtcatat cggattggga aacatacaaa gccatcagtt gggcaacctg atgattcaac
     3961 aggcggccat taaaggaaat atcggctaca ttgtccgctt ttccgatcac gggcacgaag
     4021 tccattcccc cttcgacaac catgcctcac attccgattc tgatgaagcc ggtagtcccg
     4081 ttgacggatt cagcctttac cgcatccatt gggacggata cgaacaccat cccgccgacg
     4141 gctacgacgg gccacagggc ggcggctatc ccgctcccaa aggcgcgagg gatatataca
     4201 gctacgacat aaaaggcgtt gcccaaaata tccgcctcaa cctgaccgac aaccgcagca
     4261 ccggacaacg gcttgccgac cgtttccaca atgccggcgc tatgctgacg caaggagtag
     4321 gcgacggatt caaacgcgcc acccgataca gccccgagct ggacagatcg ggcaatgccg
     4381 ccgaagcttt caacggcact gcagatatcg tcaaaaacat catcggcgcg gcaggagaaa
     4441 ttgtcggcgc aggcgatgcc gtgcagggta taagcgaagg ctcaaacatt gctgtcatgc
     4501 acggcttggg tctgctttcc accgaaaaca agatggcgcg catcaacgat ttggcagata
     4561 tggcgcaact caaagactat gccgcagcag ccatccgcga ttgggcagtc caaaacccca
     4621 atgccgcaca aggcatagaa gccgtcagca atatctttat ggcagccatc cccatcaaag
     4681 ggattggagc tgttcgggga aaatacggct tgggcggcat cacggcacat cctatcaagc
     4741 ggtcgcagat gggcgcgatc gcattgccga aagggaaatc cgccgtcagc gacaattttg
     4801 ccgatgcggc atacgccaaa tacccgtccc cttaccattc ccgaaatatc cgttcaaact
     4861 tggagcagcg ttacggcaaa gaaaacatca cctcctcaac cgtgccgccg tcaaacggca
     4921 aaaatgtcaa actggcagac caacgccacc cgaagacagg cgtaccgttt gacggtaaag
     4981 ggtttccgaa ttttgagaag cacgtgaaat atgatacgaa gctcgatatt caagaattat
     5041 cggggggcgg tatacctaag gctaagcctg tgtttgatgc gaaaccgaga tgggaggttg
     5101 ataggaagct taataaattg acaactcgtg agcaggtgga gaaaaatgtt caggaaacga
     5161 gaagaaggag tcagagtagt cagtttaaag cccatacgca acgagaatgg gaaaataaaa
     5221 cagggttaga ttttaatcat tttataggtg gtgatatcaa taagaaaggc acagtaacag
     5281 gagggcatag tctaacccgt ggtgatgtac gggtgataca acaaacctcg gcacctgata
     5341 aacatggggt ttatcaagcg acagtggaaa ttaaaaagcc tgatggaagt tgggaaatga
     5401 aaaaaggaag gggcggaaaa cctatgacaa atcattctat gttcccaaaa gattgggatg
     5461 aggctagaat tagggctgaa gttacttcgg cttgggagaa aagaaaggaa ttagaaggtc
     5521 aaaaatggag aggtacaagt aaatctggtg ttgaaataga aggatttacg gaacctaatc
     5581 gaacagctta tccactttat aatagtaaat gaagtattga ttaaaaaggt gtttttaaat
     5641 gtataaaata aaatgttttt atgatcagtt tagttctggt gaattattta actactgcca
     5701 gtttttagat aattcttcta atcaaaagat aaatacacga ggaacggtgt atcaatatat
     5761 tatttatgtt ttaacaggag acctttatct acaaaaagac atagatgaaa atttagaatt
     5821 tatccatcaa gcagaaaata atcccaatga agtttattca ggaggtggac aaggtttttg
     5881 ctgggatatt tctgctgaaa aagtagtttt ctatcacaac gaatttgatg aagaagacgg
     5941 ctggcctgat ttaagctgtt cgctgcatac ttttaaaact gctttaattg cttggaatgc
     6001 ttttttgcaa ttgcctaaaa gtattcattc ggtggtggag actgtgattg aggaataagc
     6061 ataattagct taatgaatag aatcagcgat atagattgga ctgcaaatcc acgcttatac
     6121 gctgtgccat gattaacagg aagagagcaa cttggatggg ctagtaggga tggtaagcat
     6181 ttaaatatat caattgatgg aaagattaca cacaaatgac taatttaaaa ttagatttct
     6241 actctgaagt tattataaaa gattcttgcc ctaatgattt gttagaaaat ggtgagacta
     6301 ttaaaggaaa aaaaggagtg gtattaggta taagcgaaga agatggtata atctatggct
     6361 atactatttt actttttgat ataaaatatt gtatatatat agataagaaa tatattatac
     6421 cgactggaaa aaaattctct cgcgatgatt tttattagct aatttaagta atttaagcaa
     6481 gttttttaga tactgaaact ggtaattatg tttatgtaag cataaggagt tcagattgaa
     6541 ggattctaca gggcaaatga ggctggcaac caaggatttg gcggaagcca ttaaacgagg
     6601 agaagtacgt agttctgctt ttacaacaaa gcaactaaag gcaatcgaaa aaggagaatc
     6661 taaaataccg gattatactt ggcatcatca tcaggatact ggacgtatgc agcttgtacc
     6721 tgaatgggaa cattctaaaa ccggtcacat aggagggacg gcaatggata aaggtaaatg
     6781 attatgtgga aaatttataa agaaaatagc accgacttaa attttgccat aggcagtata
     6841 tattgccaag caatcaatct taccgaattt aaaatgtggg tcgaaaaaat cataagggaa
     6901 gtggatttag atgaaattcc aaattatttt tttgatttga tagattttca atcactatac
     6961 gatctgattg atattatagg atttgttccc gaaaataatt tatcaaaaaa tcaggataat
     7021 gcattaacag gtattgcttt cttaagaggg atagatgtct atgaccctcc cgtttcaaaa
     7081 gaaaaagcat taaaagcctt aaagaaacat cctgaaattt atcagaggtt tcagcatttc
     7141 ttcccatttg tagaactgcc cccgctttaa acagtcaaaa tgccgtctga aacgatattc
     7201 ggcgttcaga cggcattttt tgatacaaag caggtaacca aaagagtgtt tgacggcaaa
     7261 ggaagataat tatgtggaaa atcataaaag aagatagtga tgatttagaa tttgcaatta
     7321 aatgcttatt ctctcaatct attgatttaa atgaattcaa gttatggatt gaacaagtaa
     7381 tacgagatat gcccatcgag gacatccctt tttatatttt tgacttggcg gattttgatg
     7441 ggggaattgc cgatattgac aatattgtag gttttgtttc gagttacagc ctatcaaaat
     7501 cgaaaaaaaa tgccttgaca ggcattgcct tcttaagggg gatatatgtc tatgatccgc
     7561 ctatttcaaa agaaaaagca ttaaaagcct taaagaaaca tcctgaaatt tatcagaagt
     7621 ttcagcattt ctttccgttt gtagaactgc ccccgcttta aacagtcaaa atgccgtctg
     7681 aaatgatatt cggctttcag acggcatttt tgatataaag cggataacta aaagagcgtt
     7741 tgacagcaaa ggaacatctg aaataccgga ttacacttgg catcatcatc aagatacagg
     7801 atgtattggt tggaaagcga tgaacaaaag aagataatta tgtggaaaat ttataaagaa
     7861 aatagcactg acttaaattt ttccctaggt agtatatatt gccaagcaat caatattacc
     7921 gaatttaaaa tgtgggtcga aaaaatcata agggaaatgg acttagatga aattccaaat
     7981 tatttttttg atctaacaga tcttcaatca ctattccatc taattgatat tataggattt
     8041 gttcccgaaa ataacttatc aaaaaatcag gataatgcat taacaggcat tgccttctta
     8101 agggggatag atgtctatga ttcgcctatc tcaaaagaaa aagcattaaa agccttaaag
     8161 aaacatcctg aaatttatca gaggtttcag catttctttc cgtttgtaga actgcccccg
     8221 ctttaaacag tcaaaatacc gtctgaaagc catttccgcc gctcagacgg cattttttcc
     8281 tctttcgttt acaaaccctt aaaatccatt tacactcaaa ctcgttcaaa acaaaacaaa
     8341 ccctgccatg aaaaccctgc tcctcctcat ccccctcgtc ctaac
//
LOCUS       DAWHRS010000087         8301 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWHRS010000087 DAWHRS010000000
VERSION     DAWHRS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.087"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WIB82_001398"
     CDS             complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WIB82_001398"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ3842687.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1275..1880)
                     /locus_tag="WIB82_001399"
     CDS             complement(1275..1880)
                     /locus_tag="WIB82_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842688.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WIB82_001400"
     CDS             complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WIB82_001400"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ3842689.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAEWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2865..3161
                     /locus_tag="WIB82_001401"
     CDS             2865..3161
                     /locus_tag="WIB82_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842690.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3175..3843
                     /locus_tag="WIB82_001402"
     CDS             3175..3843
                     /locus_tag="WIB82_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ3842691.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3916..6039)
                     /gene="pnp"
                     /locus_tag="WIB82_001403"
     CDS             complement(3916..6039)
                     /gene="pnp"
                     /locus_tag="WIB82_001403"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ3842692.1"
                     /translation="MMFDKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVA
                     VTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNG
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWS
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTS
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKI
                     REVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6273..7136)
                     /locus_tag="WIB82_001404"
     CDS             complement(6273..7136)
                     /locus_tag="WIB82_001404"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ3842693.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDENGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7336..8199
                     /gene="rfbD"
                     /locus_tag="WIB82_001405"
     CDS             7336..8199
                     /gene="rfbD"
                     /locus_tag="WIB82_001405"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ3842694.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALSAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FVQHIFQAASQQQTSFPIPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1997 a         2394 c         1920 g         1990 t
ORIGIN      
        1 atatttccct caagcaatgc ggcggcacca atacctgatt aaaacacaac cggtgttttc
       61 ggcatatatg caaaaacgca ttttccggca cggcgttaca ccgataccga aaaatgcgtc
      121 tgaatgtttc gacaatccga ttaaagcatt atgccaaatc tgcaaaaagt ggcgtagaga
      181 gatagcgttc gccataagaa ggcagcagca cgactatcag cttgccttcg ttttcaggct
      241 gtttggcaag ctgcaacgca ctccaaaccg ccgcaccgga agaaataccc accaaaatgc
      301 cttctttttc cgctattgcg cgggcggttt caaaagccgc ttcgttcgac actttggtaa
      361 tgctgtcgta aattttggta ttcaaaacgg tcggaataaa gcctgcgccg atgccttgaa
      421 tcgggtgcgg gcctttttcg ccgccgctca atacgggtga agcttcaggc tcgacggcaa
      481 ccactttaac ttcgggtttg tattttttca acacttcgcc cacgccggta atcgtaccgc
      541 ccgtgccgac gccggcaacg aagacatcga ctttgccgtc cgtatcccgc caaatttcct
      601 cggcggttgt tttgcggtgg acttcggggt ttgcctcatt gtcgaactgg cgcggcataa
      661 aataagtgtc gggatgcgcg tccaccaagg atttcgcttt ggcaatcgcg cccgccatac
      721 cttcggcggc aggggtcaga atcagctccg caccaaacgc gcgcaacagc attttgcgct
      781 ccttactcat gctttccggc atggtaatcg ccagcttgta gccgcgtgcg gcacatacca
      841 ttgccaaacc gacacccgta ttgccgctgg ttgcttcgac aatgacggtg tttttgttga
      901 ttttgcccgc tttttcggca ccctcgatca ttgcttcggc aatgcggtct ttgacgctgc
      961 tgcctggatt gaaaaattcc agtttcacgg caacctccgc cttcaaacct tcggtcagac
     1021 ggttcagttt gaccaaaggc gtgttgccga ttagttcggt gatgctgttt gcaattttca
     1081 tcttctgctc ctgaataata aggtttcgac agtaaggcgc aatccccata ggcatcctgt
     1141 ccgattgcag gggatttgcg ccgaaacata aacaaatttc ggatatggct ttatcctaaa
     1201 cctatgccgc ctaaaaaacc aatatccaaa atttctttct ttataacttt ttgttccgat
     1261 acggccgcac cgtattatct tccaatttct gaaagcagca gcaccagtac cgagacctgc
     1321 atcaacgctg ccgtcaaccc gccgagtcgc agctgcgagc cgccgaacgt tgacggcggc
     1381 tgccggtcta taccgcagcg gtggacatag gcaaccgcca tcagcacaaa ctgcaacacg
     1441 aaccaataaa ccggcttcag cgcataaagt ttgagcagtg ccgcaaacac aaacacttga
     1501 accgttcccg aaaagcaata atgcaccaac gcacatattc cgacaaaagc aagcgtcatc
     1561 gatacattac tgacggcaaa aagcccgagc agcggatgtt cggggtctgc ctgccatccg
     1621 tttccatctg ttttccggtt ccaatgcccg atgaaaaaaa atatgctgcc caaagtcagg
     1681 taaaaatggg ggatgaacaa gggcgcggcg cgggtcattc cccatatgcc gggcatcagc
     1741 tttgccccca aaaccgatat gcccagccac agcataatac tcgcccaaaa ccattgcaac
     1801 attcctgcgc ggaatatcag aaacagaaaa accgccgcat acagtaaggc agacaaaaaa
     1861 tccaaatgca gcaaaatcat gaatacgcca actcaccttc aaacaccgct tccgcagggc
     1921 cggtcatcat cacatcgccg ccgcaggccc attcgatata taaagtcccg cccggcaaaa
     1981 ccacctctac cgttttccct tcatccaaca gccccagacg gatacccgcc accacagccg
     2041 cacacgcgcc cgtaccgcaa gcttgggttt cgcccacgcc gcgctcgaac acgcgcaaac
     2101 gaatcgcggt tcggccgaca acctgcataa agccgacatt gacgcgttcg ggaaactgcc
     2161 tgtgcggttc gataagcgaa ccggtttcgc gcaccggcgc gcattccacg tcatcgacca
     2221 caatcaccgc atgggggttg cccatattga cgcagctgac aggctgaatg ccggattcga
     2281 gatgcacccc gtaaatacag gcatcatccc cctcgcccga ttcggggaca aacggtattt
     2341 cagacggcat aaacttcggt ttgcccatat tgaccgtaac cataccgtta tcggacaatt
     2401 tcggaaaaat aacgccattt gccgtttcaa cacaaatttc tttcttatcg gtcaaaccct
     2461 tgtctgcaac aaaacgggca aaacaacgcg ctccgttgcc gcattgcccg acctcgctgc
     2521 cgtcggcatt gaaaatacgg taacggaaat ccacgccttc ggtttccgaa cgcccgacca
     2581 ccaaaagctg gtcgaagccc acgccccgga agcggtccgc ccattccgca atcggcgcgt
     2641 cctcgggggt aaaatcctga ctgaccgcgt caatcaccat aaaatcgttg cccaaaccgt
     2701 gcattttggt aaatttcaaa gtgttcatat tgttccgaaa tcccgtatcg tctgttgctt
     2761 ccgaatccga aatcccccgc accgaaggga aatgcttcca tctcccgccg cctgaggatt
     2821 aaaatcagcc attatactgt ttgttacccc ttacaagaaa caccatgttc cgcatattac
     2881 tgttgctttt cccttgtgca atccttgccg cctgcacccg aaacataccg gcaaacccgg
     2941 ccgtttcaaa ttgcgcgcat cttcaaaacc gcaccatcct gtattttgac gcacacggca
     3001 aacccgccga cggcgcagta aagggcggat tttacaaaat tatcgaaata cagggtgcaa
     3061 actgcatcct tattcaagac tttaacagca cgggcaatgc cctcggcgga tcctatgtgc
     3121 cggactgccg caaatcatcc cttttcccga aaaatatttg acttcaaact gcctatgaac
     3181 cacgacatca ctttcctcac cctgttccta ctcggtttct tcggcggaac gcactgcatc
     3241 ggtatgtgcg gcggattaag cagcgcgttt gcgctccaac tccccccgca tatcaaccgc
     3301 ttttggctga tcctgctgct taacacagga cgggtaagca gctatacggc aatcggcctg
     3361 atactcggat taatcggaca ggtcggcgtt tcactcgacc aaacccgcgt cctgcagaat
     3421 attttataca cggccgccaa cctcctgctg ctctttttag gcttatactt gagcggtatt
     3481 tcttccttgg cggcaaaaat cgagaaaatc ggcaaaccga tatggcggaa cctgaacccg
     3541 atactcaacc ggctgttacc cataaaatcc atacccgcct gccttgcggt cggaatatta
     3601 tggggctggc tgccgtgcgg actggtttac agcgcgtcgc tttacgcgct gggaagcggt
     3661 agtgcggcaa cgggcgggtt atatatgctt gcctttgcac tgggtacgct gcccaatctt
     3721 ttagcaatcg gcattttttc cctgcaactg aaaaaaatca tgcaaaaccg atatatccgc
     3781 ctgtgtacgg gattatccgt atcattatgg gcattatgga aacttgccgt cctgtggctg
     3841 taagccccat tcattcccga tacccgtaaa aaataccgtc tgaaacctgt tcagacggca
     3901 ctttcaccct aagcgttact cggcggcatt ttcctcacgg gcaggcgcgt ccagcagggc
     3961 tttgatggac agacggacac ggcctctgtc gtccacttcc aatgctttca cgttcaccac
     4021 ctgaccgact tgcaggtagt cgccgacatt gcgtacgcgc tcgtgggcga tttggctgat
     4081 gtgtaccaaa ccgtctttgc ccggcatcac gctgacaatc gcgccgacat tgttgtcgag
     4141 gattttcacc acagtgcctt cgtacacttt gcccacttcc acttcggcag taatctgctc
     4201 gatgcgtttt ttcgccgcat cgccggcttc ttgagtggtt gcggcaatgg taatcgtacc
     4261 gtcttcggca atattgattt ccgtaccggt ttcagcggta atcgaacgga tggtttcacc
     4321 gcccttaccg ataacttcgc ggattttgtc ttggttgatt ttcatcgtga acaagcgtgg
     4381 cgcgtgtgcg gacagctctt gcgggcccgc aacggcggct ttcatctgat ccaagatgtg
     4441 cagacgcgct tctttggcct gtgccaaagc gatttgcata atttctttgg taatgccttg
     4501 gattttgatg tccatttgca gcgcggtaac gccttcggtc gtaccggcca ctttaaagtc
     4561 catatcgccc aagtggtctt cgtcgcccaa aatgtcggtc aggacggcaa atttgttgcc
     4621 ttccagaatc agacccatcg cgataccggc aacgtgtgct ttcaaaggca cgccggcaga
     4681 cagcaggctc aggcagccgc cgcagacgga agccatagag gaagagccgt tggattcggt
     4741 aatttcggag accacgcgca tggtgtagct gaaatcttca ggtttcggca atacggccaa
     4801 caatgcacgt ttagccaaac ggccgtgacc gatttcacgg cgtttcggtg cgcccatgcg
     4861 gcccacttcg ccggtagagt acggcggaaa gttgtagtgc agcataaagc ggtcggtgta
     4921 ttcgccggac agcgcgtcga tgatttgctc gtcgcgcgaa gtacccaaag ttgcaacggc
     4981 caaagcttgg gtttcgccac gggtaaacaa tgcagaaccg tgcgtgcgcg gcaatacgct
     5041 ggtttggatg ttcagcggac ggacggtgcg ggtgtcgcgg ccgtcgatgc gcggttggcc
     5101 atccaaaatt tggctgcgga cgacatcggc ttccaagtgt ttgaaaatgc ctttgatttc
     5161 gttggctgcc aaagtgtcgg tttcttcggt aatcaaggct tcttttaccg cactccaagc
     5221 ttcgtccaat ttggcagaac gcgcttgttt ttgacggatt ttgaacgctt ctttaatggt
     5281 ttcgccggca atcccgcgga ctttggcaac cagttcctca ttggtttcag gtgctttcca
     5341 atcccaaagt tccggattga cttcgtcggc aaattcattg attgcattga tggcaacctg
     5401 catttgatcg tggccgtaaa ccaccgcgcc cagcatcacg tcttcgggca ggattttggc
     5461 ttcggattcc accatcaaca cggcttttga agtaccggcg accaccaagt ccaattgcga
     5521 tttcgccaat tcggctttag tcggattcaa aacgtacacg ccgtttacat aaccgacgcg
     5581 tgccgcgccg atcgggccgg caaacggtac gccgctcaac accagcgcgg cagatgcacc
     5641 caacattgca ggaatatcag aatcgatttc aggatcgacg gacacgacca tcgctacgat
     5701 ttggatgtcg tggtagaaac cttcagggaa cagcggacga atcggacggt cgatcagacg
     5761 gctggtcagg atttcttttt cgctttgttt gccttcgcgt ttgaagaaac cgccgggaat
     5821 tttgcctgcg gcgtaagtgc gttccaaata atcgacggtc agggggaaga agtcttgacc
     5881 ttctttcact tctttgttgg tggtaacggc aaccaaaaca acggtgtcgc ccatagagac
     5941 tttaacggca gcggcggctt ggcgggcaat ttcgccggtt tccaaagtaa cggtctgatt
     6001 accgtattgg aaggtcttaa cgtgtttgtc gaacatcatt gttcctttca aaataccgca
     6061 ctgctaaaac actaataatg cacactaaaa tccgaatgtg catagttagg gtttcagacc
     6121 gtgcggcagg ttataaacaa gctttcagac ggcatttcgg acgctgaaag caattgcgga
     6181 ttataaaggc aaccatcctt aaaatccagt attgatacaa taaaaaggcc gtctgaacca
     6241 tatttctttc agacggcctt caaacttaaa aatcaatcct gagtcagcaa agtcaatgct
     6301 tcccgatact tctcgacagt tttctgaatc acatcggcag gcactttcgg cgcaggggct
     6361 tttttgttcc aaccgctttg ttccagccag tcgcggacga attgtttgtc aaaagacggc
     6421 ggattggtgc cgactttgta ttggtcggca ggccaaaaac ggctcgaatc gggagtcaat
     6481 acctcatcca tcagcgtcag cgtaccgttt tcatccaaac cgaattcaaa tttggtatcg
     6541 caaataataa taccgcgcga tttggcatat tccgccgctt cggtgtaaag ccgaaccgcc
     6601 ttggcgcgca cttcttccgc caattctttg ccgataatgc gtccgcattc ttcaaagctg
     6661 atgttttcat cgtgatcgcc gactgcggct ttggttgagg gcgtaaaaat cacttcaggc
     6721 agttgttgcg cttcctgcat accttcaggc agttgaatac cgcaaaccga gccggttttt
     6781 tgataatctt tccaaccgct gcctgccaga taaccacgca caatcgcctc tactttcacc
     6841 ggagtgagct ttttagccac gacggcgcgt ttctctaaag ctttggcttc gttttcaggc
     6901 aaaacatcgt aaaccgtttg accggtaaag tggttgggca taatatgcgc cagtttttta
     6961 aaccaaaaat tggaaatctg cgtcagaatc tcccctttgc tcggaatcgg gtcgtccaaa
     7021 atcacatcaa acgcggacag gcggtcggaa gcgaccatca gcatacgttt atcgtcgatt
     7081 tcatataaat cgcgcacttt tccagaatag atctttacca aaccaatctc actcatttcg
     7141 cacccccctg aaaatatctt gaaaataccg acccgacacc cgacaggttt gaatcacaaa
     7201 ccgatattct aaccgaagtc ggcgcaaaac aatacccatg gcacaaaaag ccaacccgtc
     7261 aaccgtcggc aaagttttgg cactataata ccgacagcaa gtcctacaat acactttcac
     7321 caaaggaaat acctcatgag aatcctattg acaggctcga aaagccaact ggcacgctgc
     7381 ctgcgcgacc gtcttccgga agactgggaa accattgcga cggattccgc atccctagac
     7441 attaccgatg ccgatgccgt ctgcaacatg gtcaaaagtt tccaacccga cgccattgtc
     7501 aacacggctg cctatactgc cgtcgacaaa gcggaaggcg atgcggcagc ggcatttgcc
     7561 gtcaatgctt ccgccgttta caaccttgcc ttgtcagcac atcgcgccca tgcccgattc
     7621 atccacatct caaccgacta tatctttgac ggtaaaggga aaagacccta tcaggaaagc
     7681 gactttacca atccttccaa tgtatacgga caatccaaaa ccgcaggcga gctgctcgca
     7741 ctgtctgcca atcccgacag ccttatcctg cggacttctt ggctgtttag cgaatacggg
     7801 gacaactttg tccgcacgat gctgaacctt gcacgggaac gttccccgct gtccgccgtc
     7861 cacaaccaaa tcggctgccc gacctatgcc ggcgatttag ctgccgccat catccgcctt
     7921 ttgcagcaaa cgtcgcccgt gcgcggcatt taccactacg ccggcggcaa atccgtatct
     7981 tggtacgaat ttgtccaaca tattttccaa gcggcatcgc aacagcagac atccttcccc
     8041 attcccgaat tgactgccgt ttcagacaag gaatatccga ccgccgcccc caggcccgca
     8101 tacagcattt tggactgccg caaaatcgaa aacgacttcg gcatcaaacc gtcagactgg
     8161 caaaaagccc ttgcgcaggt cgtttccaag ctgctctgat gccgcccgcc tctccgtttc
     8221 cgccgtcaag caccgccttg gcggttttct tatatagtgg attaacaaaa accagtacgg
     8281 cgttgcctcg ccttgccgta c
//
LOCUS       DAWHRS010000088         8284 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWHRS010000088 DAWHRS010000000
VERSION     DAWHRS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.088"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(90..1346)
                     /locus_tag="WIB82_001406"
     CDS             complement(90..1346)
                     /locus_tag="WIB82_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ3842695.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEEIEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1383..2240)
                     /locus_tag="WIB82_001407"
     CDS             complement(1383..2240)
                     /locus_tag="WIB82_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ3842696.1"
                     /translation="MNYALDALWWKLAARPVRDLASLLTAPPLWQRSCELGVRELLGE
                     HGFRYLLALDADPTPLTDYLARRAPFGHRLGIYAENLLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVARLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(2304..3122)
                     /locus_tag="WIB82_001408"
     CDS             complement(2304..3122)
                     /locus_tag="WIB82_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ3842697.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFHPDDVQSFVLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEAQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDMPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRHMQSLFGRANTQTEDIDILRGFFNTLQQHLKDRQP"
     gene            3186..3356
                     /locus_tag="WIB82_001409"
     CDS             3186..3356
                     /locus_tag="WIB82_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842698.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            3366..4151
                     /locus_tag="WIB82_001410"
     CDS             3366..4151
                     /locus_tag="WIB82_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ3842699.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDSWLESGDIVAANPKVLAQMLKIISAHV"
     gene            4421..6516
                     /locus_tag="WIB82_001411"
                     /pseudo
     CDS             4421..6516
                     /locus_tag="WIB82_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            6640..8190
                     /locus_tag="WIB82_001412"
     CDS             6640..8190
                     /locus_tag="WIB82_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ3842700.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2007 a         2040 c         2315 g         1922 t
ORIGIN      
        1 cgtactgatt tttgttaatc cactataatt gatgaaatgc cgtctgaacg ctttgaaaag
       61 ggttcagacg gcattttgtt caaaccggtt tatttgcgtt gcaatacggc ggcgaagaag
      121 ccgtcggttt ggtgtcgggc ggagttgagg cgcaggtatt tgccggtatc caaatcgatt
      181 ttcaaaccgg caagcagttc ggcgcagttg acgggttcaa attcgggatg ttcggacagg
      241 aaacgttcga cttgcagctc gttttcttcg ggcaggatgc tgcaagtggc gtaaaccaag
      301 cggccttgcg gttttaccag tttggaggca gcatcgagga tgctgtgttg ctgttccaaa
      361 aggttggcca cggtctcggc agattggcgg tatttgaggt cgggattgcg gcgtaaagta
      421 cccaaaccgg agcagggcgc gtccaccaac acacggtcgg ctttgcctgc cagtcgggcg
      481 atacggctgt cgtgttcgct gctgatgcgt tcggggtgga tattggtcag tccggcgcgg
      541 gtcatacgcg gtttgaggtt ggcaaggcgt ttttcggcga tgtcgaaggc gtagattctg
      601 cctttattgg ccatttgcgc accgacagcc aaggttttgc cgccggcacc ggcacagaaa
      661 tcgacaatga tttcgccgcg ttttgcgccc accaataaag caagcagctg gctgccttcg
      721 tcttggactt ccagtgtgcc gtccaaaaac agctcgtgtt tgttaagcgc gattttgttt
      781 ttcaggcgga tgccccaagg cgaataaggc gttgcctctg catcggcact ttcggcttgc
      841 aacaatggca gcactttatc gcgtttgcct ttcaaggtat tgacgcgtat atcgagcggc
      901 gcagcttggt tgatgctgcg gccgaaggcg aggatttctt cttcgcgcca atgctgctgc
      961 agctgttcta ccagccattg cggcaattct gcggcggtat tcaggccgtc tgaaaactcg
     1021 gttttacggg ctttcaaatt gccgagaaac tctatttctt cttcatcaag caggtctttg
     1081 atttggctga tgttggtgct tctgccgaga accagtgcgg cgagtgcggc tttgcgcggc
     1141 tgcgcgtgcg gacggcgcag ggcggtactg attttttgat agtggcgcag cgcggcaaag
     1201 gcggtttcgg cgatttcgtg gcggtcttgg cggccgagct ttttatgttc gcggaaatag
     1261 gcggagagga cggcatcggc aggctgtttg aaagtcagca tttcagccaa aactttggcg
     1321 gtatggtcga gttgtgcggc gttcataatg taatgggttt ctcttaaagt tggtaaatgg
     1381 tttcagacgg ccttcataat gcgttcgatt tcgtgccaaa agccgtccgg ccgacattcc
     1441 aaaacggcaa tcaagccttg gtcgatacgg cggatttcgg ttgcgttcaa tgtttcggtt
     1501 tcggcgacac gcgcaggcgc gaggtaggcc atacggtcga gcagatggta gcggacttct
     1561 tggcgtttaa acccgtattc cgcccaatct tgaatataga tgccgcgcca accgtatgga
     1621 ttaagcggtg gctcaaaagc atggaaaccg tgtggaaaaa atccgatgcc gcgcacgatg
     1681 gaaacgggtt ttacgtcaag cggcagaccg tgttgccgca aggttcggat gccgtctgaa
     1741 gtgtgcggca gaccgagttg ggcggtcagt ttggcggctt ttcccaacag cgtgtctttg
     1801 gggtcgagtc cgcgcatgtc ttcgagcctg cccgtgccgc cgccgtaata tttgcaggtc
     1861 agctcgatat ggtagggttt gccgttaagc cttgccacaa aatccgccgc gccttgcgta
     1921 ttgccgtccg aaccggaaac cgtgaggttg tgcgcgagca gttcggcgtg tggcgcgttg
     1981 gcaaaccaaa aagccagcag attttcggcg taaatgccga gacggtgtcc gaacggggcg
     2041 cgtcgggcaa ggtaatccgt caatggcgtg ggatcggcat ccaatgccaa aagatagcgg
     2101 aaaccgtgtt cccccaatag ttcgcgcacg cccaattcgc aactgcgttg ccataaaggc
     2161 ggcgcagtca gcagcgaggc gaggtcgcgg acgggtcggg cggcgagttt ccaccataat
     2221 gcgtctaggg cgtaattcat tgtttttccg tattctgcct gaccggaaat gccgtctgaa
     2281 aaccggattc ggacggcgcg gatttaaggc tgcctgtcct tcagatgctg ttgcagcgta
     2341 ttgaaaaaac cgcgcaggat atcgatgtct tcggtttgcg tgttggcgcg tccgaacagg
     2401 ctctgcatat ggcgcatcag acgctcgccg ttgcggcggt tgaaaaagcc gatgtcgttc
     2461 atcacgcttt ccatgtgggc gaccatgcct ttgatttgct cgtgggtcgc ggcgtggtct
     2521 tcctgttgaa gatgggtcat gggcatatcg gtctggctga agatttcgta gcacacgacc
     2581 tgcacggctt gggcgaggtt gagcgagaaa tagtcgggat tgccgttgat ggtcatcagt
     2641 cggttgcagg cttgggcttc ttcgatgctc aagccgaaag tctcgttgcc gaaaaccagc
     2701 gccactttct cgccgcggtt ggcggcctgt agtaattcgg gcaccaaatc gcgcggcgtt
     2761 tgcagcggcg cggtgatttc gcggcggcgg ctggtcaggg cgcaggcgat ggtggtgtcg
     2821 gcaagggctt cgtccaaaga agcggcaatg gtggcatttt ccaaaacgtc cgccgcgccc
     2881 gaagcgagga tgaaactttc ttcgggcagt acaaaacttt gcacgtcatc gggatggaaa
     2941 acgggcgggt tttccgtcat cggcgtagcc atcagattgg gggcgacgat ggtcagtttg
     3001 tgcagaccca ttgttttcat cgcgcgcgcg gcagagccga tattggcggg atggctggtg
     3061 cgtgtgagga tgatgcggat gttgtccaga taagcgggca gggcgggttt gagagtagtc
     3121 atgttcggtt ttcggtcgga atggaatagg gtataatacg ccgtcttttt cgttcggacg
     3181 gtcaaatgcc gtctgaaatg ttctttaaca acgtcggaaa tttaacgttg cgcctgaagc
     3241 agacacgcgt cgggcggtat tttacccgat ttggcaggat ttaccccaac caaataaact
     3301 ccgattttgc cgctccgaag gactgcgcgt ccgaaccggc ggtatgccac ccttaaggaa
     3361 atctgatgaa tccgtttttg aatacagcct ttaaagccgc ccgccgtgcc ggtcagatga
     3421 tgatacgcgc cgcgggcaat ctcgatgccg tcaaaaccga cagcaaagcc ttcaacgatt
     3481 ttgtttccga tgttgaccgc aattccgaaa tcatcctggt tgaggctttg aaagaagcct
     3541 atccgcacca caaaatcact tgtgaagaaa gcggctccca cggcaaagcc gcagccgagt
     3601 acgaatggat tatcgatccg ctcgacggca cgaccaattt ccttcacggt catccccaat
     3661 acgccatctc tatggcgctg ctgcacaaag gcgtgttgca agaagctttg gtttacgccc
     3721 ccgaacgcaa cgacgtatat atggcttcgc gcggcaaagg cgcgttgctc aacgaccgcc
     3781 gcatccgcgt ttccaaccgc atcgaattga accgctgcct gatcggtacc ggcttccctg
     3841 ttgtcgatca aagcatgatg gacaagtatc tggcgatttt gaaagatttc ttggcaaaaa
     3901 ccgccggtgg ccgtcgtgaa ggcgcggctt ctttggattt gtgcgccgtt gcgactggtc
     3961 gttttgacgg ttttttcgag ttcaacctca aaccgtggga cattgccgca ggtgcattga
     4021 ttgttcagga agcgggcggt atcgtaaccg atatgacggg tgaagacagc tggctggaaa
     4081 gcggcgatat tgtggctgcc aatcccaaag tgctggcgca aatgttgaaa atcatttccg
     4141 cacacgttta aatttcagcc cgctcccggg aatgcgggaa aacgggcggg tatgccgtct
     4201 gaagtatcgc gccgatattt cagacggcat ttttactggc agcgggggca ggaaggtgga
     4261 aacggctaaa tgcccgaaac tgccgaagat ggaaacccat atctgattaa acggagaacc
     4321 ctgtacgatt tgtttatagt ggattaacaa aaaccagtac ggtgttgcct cgccttagct
     4381 caaagagaac gattctctaa ggtgctgaag caccaagtga atgggcaaag ccaacgcctt
     4441 taccgcaccg attaccgtcg tcaattacga cgaacaagcc ctcaataaca ccgaagcgcg
     4501 tactttggtg gatgccgtag cgaaaaaaga cgcttccgtc tggcagttcg gcggcgaaag
     4561 caacacgctg accggactgt atttcagagg ttatcagctt gactcgcgcc aattcagcgt
     4621 caacggtttg gcaggtatgt acggcacgca aggcacggcc agcgtgcaag ttggatcggc
     4681 acaactgatt aaaggcgcat ccactgccgt aaacggcatg gaccctgaag gtgcggtatc
     4741 cggttccgtc aatatcgaaa ccaaaaaagc cgccgatgaa ggcaaccgca aaatcggttt
     4801 gggctggttc agtaacaacc gcgcccaagg tacgttcgac ttgggccaac gtttcggcga
     4861 aaacaaagca ttcggcgtgc gcgccaacgg caaactgcgc cacggcgaca ccccgcgcca
     4921 cggttacagc gaagacaaca aagaatttgc ggtaaatgcc gattatcgcg gcgaaacact
     4981 gcgcgtagcg ttcgattcca tctacgcaaa acgcaaaacc aacggcggcc gcgcgcgtat
     5041 gcaggatatt caaaacgccg gcggccgctt gtttgacgcg ccagacggca aaatcaacct
     5101 gctgctttcg tggaactggc aaaacaccgt cggcgaaacc aatatgctga cctttgaatg
     5161 ggatgcgttt gacaatgccc aaattaccgg cggtatcggc tacaacaagg cgcgttatta
     5221 cggcacactg atttccccga ccgtttgcgg tacaagcggt gcgagcgacc aaaccgctac
     5281 ctgcacaagt gccaagcaat accacaccgg cacggcgagc ctgaccgacc aatatttccg
     5341 cacattgagt atgaacctga ccgcacgcgg cgaatttgaa accggcccgg taacacataa
     5401 ctggagtatg gctttcgacc gcgttatccg ccagcggaaa accataaacg gcagcgggaa
     5461 cggcaacagc aagatagaag tgaaggcaaa cgaaaatatt gcgaatcaat tggcatcgtt
     5521 tacagccgac tatcccaatt cttgggagaa ctctgccaat ttggatgcca atatcaaagt
     5581 caacagcctg gctttgtccg acaccttggg cttcgtggat aacaaatacc gcctgacttt
     5641 gggcggacgt ttccaaacag tcgaatatac cgacaaaaaa tcacagagcg gcgatgccaa
     5701 acgtttcagc ccgatgctga tggcggcgtg ggtgccgcaa cctgatttgg ttgtgtacgg
     5761 caactatatg gaagacttgg agcctgccga catcaaaacc gatgattccg gcgaaacgac
     5821 aatggcgaaa ccgcgtgtca gccgccagtt tgaagtgggc gtgcgtaaaa actggggcaa
     5881 tttcgtaacc acgttaaacg cgttccaaat caaacgcccg gctactggcg cggtcataca
     5941 actaccaaga caacaaaagg ggtaacaaca acgctgacct atggcaacaa ttccgatttc
     6001 gcccgctata aggcgcaagg cggcgtagcc ggcgacgagc aaggtatgga acgcaatcgc
     6061 ggtatcgagt tcaatgccta tgctaacctg ttaaacaaaa ccctgcgtcc gactttgggc
     6121 ctgatgtatc tgcaatcgac cgtgaaagac tacccaaatt cacgcgatat gctggttaac
     6181 ggcgtacaag tcgccaatcc gcgcgtaatt gccaaggcag gtttggaatg ggatacaccg
     6241 tttgccaaag gcttgacttt aaacagcaat gtttcgtatt tcggcaagtc ttaccaagac
     6301 acgcaaaaac aatacgcctt cccatcctat accttggttg atgtaggcgc gcgctacaaa
     6361 accaagctcg gcaaaaatac gttgaccgtc agcagctcgg tggaaaatct gttcaacaaa
     6421 aactactggc aggtacagcg cggccaatac gaccgcagct tcgccgtcgt cggtctgccg
     6481 cgtacttact ggctgaaggc ggaattggat ttctgatttc tccgcatctg gaaaatgccg
     6541 tccgaacagg tttcagacgg catttctttt aaagagatat ggcgggatac gcttaaataa
     6601 tgctattcta gcgtctttga aacctgcttg tttgaagtga tgaaaaaacc tttgatttcg
     6661 gttgcggcag cattgctcgg cgttgctttg ggcacgcctt attatttggg tgtcaaagcc
     6721 gaagaaagct tgacgcagca gcaaaaaata ttgcaggaaa cgggcttctt gaccgtcgaa
     6781 tcgcaccaat atgagcgcgg ctggtttacc tctatggaaa cgacggtcat ccgtctgaaa
     6841 cccgagttgc tgaataatgc ccgaaaatac ctgccggata acctgaaaac agtgttggaa
     6901 cagccggtta cgctggttaa ccatatcacg cacggccctt ttgccggcgg atttggcacg
     6961 caggcgcaca ttgaaaccga gttcaaatac gcgcctgaaa cggaaaaagt tttggaacgc
     7021 ttttttggga aacaagctcc ggtttccctt gccaataccg tttattttaa cggcagcggt
     7081 aaaatggaag tcagtgttcc cgccttcgat tatgaagagc tgtcgggcat caggctgcat
     7141 tgggaaggat tgacgggaaa aacggtttat caaaaaggtt tcaaaagcta ccgcaacagc
     7201 tatgatgcgc ccttgttcaa aatcaagctg gcagacaaag gcgatgccgc gtttgaaaaa
     7261 gcgcatttcg attcggaaac ttcagacggt atcaatccgc ttgctttggg cagcagcaat
     7321 ctgaccttgg aaaaattctc cctagaatgg aaagagggtg tcgattacaa cgtcaagtta
     7381 aacgaactgg tcaatcttgt taccgatttg cagattggcg cgtttatcaa tcccaacggc
     7441 agcatcgcac cttccaaaat cgaagtcggc aaactggctt tttcaaccaa gaccggggaa
     7501 tcaggcgcgt ttatcgacag tgaagggcag ttccgttttg gcacactggt ttacggcgat
     7561 gaaaaatacg gcccgctgga tattcatatc gctgccgaac acctcgatgc ttctgcctta
     7621 accgtattga aacgcaagtt tgcacaaatt tctgccaaaa aaatgactga ggaacaaatc
     7681 cgcaatgatt tgattgccgc cgtcaaaggc gaggcttctg gactgttcac caacaatccc
     7741 gtattggaca ttaaaacttt tcgtttcacg ctgccgtcag gaaaaatcga tgtgggcgga
     7801 aaaatcatgt ttaaagattt gaagaaggaa gatttgaacc aattgggttt gatgctgaag
     7861 aaaaccgagg cagacatcag aatgagtatt cctcaaaaaa tgttggaaga cttggcggtc
     7921 agtcaggcag gcaatatttt cagcgtcaat gccgaagatg aggcggaagg cagggcaagt
     7981 cttgacgaca tcaacgagac cttgcgcctg atggtggaca gtacggttca gagtatggca
     8041 agggaaaaat atctgacttt gaacggcgac cagattgata ctgccatttc tctgaaaaac
     8101 aatcagttga aattgaacgg taaaacgttg caaaacgaac cggagccgga ttttgatgaa
     8161 ggcggtatgg tttcagagcc gcagcagtaa tgggtcaaat gccgtctgaa atgttcaggc
     8221 ggcattttgt tgtattggaa actgccgttt tcaaccattc ctttagataa gccgtgtcgg
     8281 attc
//
LOCUS       DAWHRS010000089         8202 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWHRS010000089 DAWHRS010000000
VERSION     DAWHRS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.089"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..563
                     /gene="rsmA"
                     /locus_tag="WIB82_001413"
     CDS             <1..563
                     /gene="rsmA"
                     /locus_tag="WIB82_001413"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ3842701.1"
                     /translation="PFADKLVIHEGDVLQFDFNGIAGKKKIVGNLPYNISTPLLFKLA
                     EVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDMEMLIDVPPESFDPAP
                     KVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNNLKELAGDDDLQAVGI
                     NPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            736..1464
                     /locus_tag="WIB82_001414"
     CDS             736..1464
                     /locus_tag="WIB82_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ3842702.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVLFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            1537..1896
                     /locus_tag="WIB82_001415"
     CDS             1537..1896
                     /locus_tag="WIB82_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842703.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            1901..2341
                     /locus_tag="WIB82_001416"
     CDS             1901..2341
                     /locus_tag="WIB82_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ3842704.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKKSYRRLKVWILWRQPKKTAETDTPS"
     gene            2373..3647
                     /gene="folC"
                     /locus_tag="WIB82_001417"
     CDS             2373..3647
                     /gene="folC"
                     /locus_tag="WIB82_001417"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ3842705.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPESLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACTLTVLECLNAKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            3660..4658
                     /gene="ftsN"
                     /locus_tag="WIB82_001418"
     CDS             3660..4658
                     /gene="ftsN"
                     /locus_tag="WIB82_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ3842706.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEYSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAVGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            4651..5148
                     /locus_tag="WIB82_001419"
     CDS             4651..5148
                     /locus_tag="WIB82_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ3842707.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWQQSYTIPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            5356..6900
                     /gene="purF"
                     /locus_tag="WIB82_001420"
     CDS             5356..6900
                     /gene="purF"
                     /locus_tag="WIB82_001420"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ3842708.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTIIARQCSDKAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            7000..7491
                     /gene="greB"
                     /locus_tag="WIB82_001421"
     CDS             7000..7491
                     /gene="greB"
                     /locus_tag="WIB82_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ3842709.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            7550..8176
                     /locus_tag="WIB82_001422"
     CDS             7550..8176
                     /locus_tag="WIB82_001422"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ3842710.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFHRPYIK
                     AIRVQTTSDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDAAKVAAFIATANRLFR"
BASE COUNT         2101 a         2364 c         2128 g         1609 t
ORIGIN      
        1 tgccgtttgc ggataaactg gtgattcacg aaggcgatgt attgcagttt gatttcaacg
       61 gcatcgcagg caaaaagaaa atcgtcggca acctgcccta caacatttcc acgccgcttt
      121 tgttcaagct ggcggaggtg gcggacgatg tcgtcgatat gcactttatg ctgcaaaaag
      181 aagtggtcga gcgtatggtt gccgcgccga aaagcaacga ctacggccgc ttgggcgtga
      241 tgctgcaata ttttttcgat atggaaatgc tgattgacgt accgcccgaa tcgttcgacc
      301 ctgcgccgaa agtggattcc gccgtcgtcc gtatgattcc ggtgaaacac cgcatcggca
      361 aggcggacga tttcgagcat ttcgccaaac tggtgaaact cgccttccac caacgccgca
      421 aaaccatacg caacaacctg aaagagcttg caggcgacga tgatttgcag gctgtcggca
      481 tcaatccgca agaccgtgcc gaacacatcg cgccggaaaa atacgtagcg ttgagcaatt
      541 atctggcggg caaggccgtc tgaaaaggga gtaagccgat gaagaaatac ctgcttttac
      601 ccctgatact gccgcttgca gcctgcctga ccaatccgaa cgaccgtttt tttgacggca
      661 gacgctacac gcttcatcat atggagctcc cgtaacgaca acctgcaacg gcaaatgccg
      721 tctgaaagaa aagacatgat caaattcaaa aacgtacaca aacatttcaa agacctgcac
      781 gtcatcaacg gcgtgaactt ggaaatcaaa aaaggcgaag tggtcgtcgt ctgcgggcct
      841 tcgggcagcg gcaaatccac actcatccgc accgtcaacc agcttgaaag catcgaaagc
      901 ggcgagattt gggtggacgg tgtcaacgtt gccgacccaa aaaccgattt gaacaaaatc
      961 cgcgaagaag tcggctttgt gtttcaaagt ttcaacctct acccgcacct gaccgtgttg
     1021 gaaaacatta ccctcgcacc gatgaaggtt aaggggcaaa atgcggaaca ggcggagaaa
     1081 aaggcaatgg agcttttgga acgcgtcgga ctggcacaca aaaaagacgc cttcccgtcc
     1141 caactttccg gcggccagca gcaacgcgtg gcgattgcgc gcggtttggc gatggaaccg
     1201 cgcgtaatgt tgtttgacga accgacctct gcactcgacc ccgaaatggt cggtgaagtg
     1261 ttgaaagtga tgaaagactt ggcggaaagc ggcatgacga tgatgtgcgt aacccacgaa
     1321 atgggatttg cccgcgaagt tgccgaccgg gtactttttg tcgataaagg gcaaatcctc
     1381 gaagacgaaa cgcccgaagc gttttttacc aacccaaaac acgaacgcgc caagcagttc
     1441 ctgcagcagg tcatgaccca ctgatgctat gccgtctgaa aaccgaacgt tcagacggca
     1501 tagcccgaca ccgcaagatg attgagaaag aaaaccatgt attttgttga ccgcaccgcc
     1561 gtcgtcctca agccgaccgc gcgctttttg gaatggctca aaagcaccga cgaaaatatg
     1621 cccgacctga ccatagaaca gcttcgtgcc aactgttccg tcttcctcgt tccgcagttc
     1681 gacgaaccgg aagccgtcgt ttcctatttc gacgaacgct accgccagat tttcgaggcg
     1741 gagctggcag gttgggacat cgataaggat aaatggccgc aagatatggg gctgaaggcg
     1801 ttttgggaat ttttcgacat cgaaatccac gatatggtgt tggacatgga agaagcggaa
     1861 ttgaacatta cccccgtgtt cgacaacatg atgtaaggct gtgcgcgcct ttcagacggc
     1921 attgaggccg tcccgcatcc tgaacatcct gactgtatcg cttcacgccg cctcccttgc
     1981 cgtctgcctg acttggtttt acggctggat gatgtggttc ggtttggcgg ctttggtggc
     2041 aagttacgca tactcgctca ggataacgaa tctgaaacac cgccatgcca taaccgccat
     2101 taccatagac cgcgacggac aggcggaaat cgtatccggc aaagacaaaa cagcggcaac
     2161 acttgcgggc agcagtatgg ttacgcctta tgccttattc ttacaatggg acacaggcgg
     2221 cacaaccgtc agccaatgca tcatacccga catgacggac aaaaaatcct accggcggct
     2281 gaaagtctgg atactttggc ggcagccgaa aaaaaccgcc gaaaccgata caccaagctg
     2341 atttcagacg gcatttgaac acggaacact caatgaaaac actgcaagac tggctctcgc
     2401 atttggaaac cgcccacagc ggcggcttga tcgatatggg cttggaacgc gtaggcgaag
     2461 tgaaaaaacg gatgaagctc acgccgcaat gccccgtcgt cgttgtcgcg ggaacaaacg
     2521 gcaaaggttc ggtctgcgcc tacctgacac agatttacaa acaggcgggc tacaaaaccg
     2581 gcacactgac cagcccgcat ttattgcgtt acaacgaacg catcgccatc aacgccgaac
     2641 ccgtttcgga cgatacgatt attgcctctt tcgagcgcat cgaagcggcg cgcagcgaaa
     2701 tatcgctgac ttattttgaa ttcaatacgt tggcggccgt cgacatcttc atgcgcgaac
     2761 aggttgacgt gatgatattg gaagtcggct tgggcggacg cttggacgcg gtcaacgcat
     2821 ttgacggcga ttgcgcggtg gttactagtg tagatttgga ccatcaggcg tttttgggcg
     2881 atacggtcga gcaggtcggc tttgaaaaag caggcgtgtt ccgcagcggc aaacccgcaa
     2941 tctgcggtca aaaccccgcg cccgaatcgt tgctttcaca cgccgaagcc ataggcgcga
     3001 aactgctgat ggtgcagcgc gatttcgagt ttcacgcgat ggaaaacatc caatggaact
     3061 accgcttccg gccgcaacat tcagacgacc ccgcgcgcaa ccgcaatgcc ctgcccttcc
     3121 ccgcattgcg cggcacatac caactttcca acgccgcctg cacgttgacc gtattggaat
     3181 gcttgaacgc caaattgccg gtggacatcg gcgccatcaa acgcggattg ctgctggttg
     3241 aaaatcccgg acgcttccaa gtcctgcccg gccgtccgct gaccgttttg gatgtcggac
     3301 acaatcccca cgctgcccgc gccctgcgcc gcagcctgat taatttggcg tttgcgcaaa
     3361 aacgcaccgc cgtattcagt atgctgtccg acaaagacat agacggcgtg ttggaaatcg
     3421 ttaaagacca gtttgacgag tggtatatcg caccgttgga tgtaccgcgc ggcatgacgc
     3481 tcgagagcct gcaaaacaaa ctgcacgaac acagcatcga aaacatacag acattcgccg
     3541 ccgtccgcga agcataccgg gcggcagcct caaaagcggg cgagaacgac agaatcgtcg
     3601 ttttcggctc atttcatacc gttgccgatg tcatgtcggt actttaaaag gaaacttcta
     3661 tgtccgaaaa caaacaaaac gaagtcctga gcggttacga acaactcaaa cggcgcaacc
     3721 gccgccgcct cgtaacggca agttgcctgg ttgccgcctc ctgcatcctg ctggcagccg
     3781 ccctcagttc cggccctgcc gaacagactg ccggcgaaac aagcggcgta gaaaacaaag
     3841 cggcaggtgc ggcacaaacc cctgccttga aatccgccgc cgacaaaccg caggacttgg
     3901 caggcgaaga caagccttct gccgccgaca gcgaaatcag cgagcctgaa aacgtaggcg
     3961 cgccgctggt gctgattaac gaccgcctcg aatacagcaa catcaaaggt ttggaagcat
     4021 ccgagaaact gcaacaggca gaaaccgcca aaaccgcacc gaagcaggca aaacaacgcg
     4081 ctgccgaaaa agtgccggca actgccgaca gtacggatac ggtagcggtt gaaaaaccga
     4141 aacgcactgc cgaaacaaaa ccgcaaaaag cggaacgcac tgccaaagcc aagcccaaag
     4201 ccaaagaaac caaaaccgcc gaaaaagttg ccgacaaacc gaaaactgcc gccgaaaaaa
     4261 ccaaaccgga tacggcaaaa tccgacagcg cggtaaaaga agcgaaaaaa gccgacaagg
     4321 ctgaaagcaa aaaaacagcc gaaaaagacc gttcggacgg caaaaaacac gaaacggcac
     4381 aaaaaaccga caaagcggac aagaccaaaa ccgccgagaa ggaaaaatcc ggtaaaaaag
     4441 ccgccattca ggcaggttat gccgaaaaag aacgcgcctt aagcctccag cgcaaaatga
     4501 aggcggtggg tatcgattcg accatcaccg aaattatgac cgacaacggc aaagtttacc
     4561 gcgtcaaatc aagcaactat aaaaacgcaa gggatgccga acgcgatttg aacaaattgc
     4621 gtgtacacgg tatcgccggt caggtaacga atgaataacc tccccgttgc cgacctcctc
     4681 gtcttcgcca tcatcgccgc ctgcatcgtg ctatccgcga tgcgcggcgt gattgcggag
     4741 gcaggttcga tggcggcatg ggtggtttcc ttcttttttg ccaaactctt tgccgcaccc
     4801 ttcgccgacc tcgcctttgc ctcgttccaa ccccgcctgt ttgcattggc tctgtcgttc
     4861 atttccctgt tcgtcattgc ctgtctgatc cagaaaatgc tccgttcgct gctgaccggc
     4921 gcagtttcgg cggtcggttt gggctttgcc aaccgcattt tgggcggcgt attcggtgca
     4981 ttgaaaggcg ttttgattgt taccctgctg gtcatgcttg cttcaaaaac cgacctgccc
     5041 gataccgaag agtggcagca gtcctatacc ataccgtttt tcgtatcgct ttccgaagcc
     5101 gtgttgaacc atagcggcgg cacggcggaa actccggaag acgattgatt gccatacgga
     5161 tatttcagac ggcattctct tttatgccgt ctgaaatgtt ttttcctttc ctcagccata
     5221 tcacagatta aatcttatcc tccataaacc cacctatctg tccttcgctc aaatattgtc
     5281 aacaattcgg gcaaaaagcc gccgtttgct ttataattcc gttttaccct taaacctcga
     5341 atcggagaag acgatatgtg cggcgtatta ggtttggtca gtcatgaacc cgtgaaccag
     5401 cttctgtacg acggtttgca gatgttgcag cacagggggc aggatgcggc gggcattgca
     5461 acggcggaag gcggcacctt ccatatgcac aaaggcaaag ggatggtgcg cgaagtgttc
     5521 cgcacgcgca atatgcgcga tttggtcggc aacgccggca tcgcccacgt ccgttatccc
     5581 acggcgggca acgccggcag cagcgcggag gcgcagcctt tctacgtcag ctcgccgttc
     5641 ggcatcgttt tggcgcacaa cggcaacctc accaacactg ccgaactgta tgaaaacgtg
     5701 tgcaacaaac acctgcgcca cgtcaacacc agctccgatt ccgaagtctt gctcaacgta
     5761 ttcgcgcacg aattacgccg cgaagtctct aaaaacgccg acccgcaccg gctcaatgcc
     5821 gacaatattt tcaacgccgt tgccgaagtc caccgcctgg tgcgcggcgc atacggcgtg
     5881 gttgccatga ttgcgggcta cggtatgctt gccttccgcg acccttacgg catccgcccg
     5941 ctggtattgg gttcgcaaac cgacagcgaa ggcaggaaat cctatgccgt cgcctccgaa
     6001 tccgtcgcct tcaacgcgct tgcctacgat ttggaacgcg atatccggcc gggcgaagcg
     6061 gtatttgtcg gctttgacgg cacaatcatc gcccgacaat gcagcgataa ggccaaactc
     6121 agcccctgcc tttttgaata cgtctatttt gcccgtcccg actccgtgat tgacggcgtg
     6181 tccgtttacc agtcgcgctt ggatatgggc gtgtccttgg cggaaaaaat caaacgcgag
     6241 ctgcccgtgg acggcatcga tgtcgtgatg cccatccccg acaccagccg ccccagcgcg
     6301 atggagcttg ctgtccacct caaaaagcct taccgcgaag gtttgattaa aaaccgctat
     6361 atcggccgca cctttattat gcccggccag gcgacgcgca aaaaatccgt gcgccaaaaa
     6421 ctcagcccga tggaaaccga atttgcaggc aaaagcgtgt tgctggtgga cgactccatc
     6481 gtgcgcggta cgaccagccg tgaaatcgtc gaaatggtac gcgcggctgg cgcgcgcaaa
     6541 gtctatatcg cctccgccgc gcccgaagtg cgctatccca acgtatacgg catcgatatg
     6601 cccacgcgcg aagagttgat tgccaatggg cgcagcgcgg cggaaatcgc cgccgaaatc
     6661 ggcgcggacg gcatcgtgtt tcaagacttg ggcgatttgg aagccgtcgt caaagcactc
     6721 aacccgaaaa tcgaatcctt cgattcgtcc tgtttcaacg gcatttatca gaccggcgac
     6781 atcgatgatg cctacctcga ccgcctgtct gccgagaaat ccggctgcgg cggtctgaaa
     6841 atccacccga gcaggatgga acacagcatc agcatcagcg atgcgggcga cgaagaataa
     6901 ggcttcccaa accgcgatgc cgtctgaaat ccgaacgccg ggttttcaga cggcatttcc
     6961 catatcggca tcacatttcc cgtttcatcc gcacacaata tgagtaccga aaccaaaaac
     7021 tacattacgc ccgccggatg gcaggcattg aaagacgagc tttatcaact ggtcaacaaa
     7081 gaacgccccg aaatcgtcca aatcgtcaac tgggcggcag gcaacggcga ccgcagcgaa
     7141 aacggcgact atctttacgg caaacgccgt atgcgcgaaa tcgaccgccg catccgtttc
     7201 ctgaccaagc gtttggaagc cgccgtcgtc gttgatcccg aattgcgcga agcgaccgac
     7261 caagtgtttt tcggcgcgac cgtcggtctg ttgcgtggcg acgggcgcga gcaaaccgtc
     7321 aaaatcgtcg gcatcgatga aatcgatacc gcgcaaaaca aaatttcttg gatttcgccg
     7381 ctggctcgtt gtctgattaa agcgcgcgaa ggcgacgaag tggttttgaa cacgccggaa
     7441 gggcgtgagg aaattgaaat cctttctgtc gaatacatca aaatcgactg atcgggaatg
     7501 ccgtctgaaa cggtgcaagc gcgccgtttc ccaaataaag gaataccgaa tgaggaaaat
     7561 ccgcaccaaa atctgcggca tcaccacacc ggaagacgca tcggccgccg tagcggcagg
     7621 tgcagatgcc gtcgggctgg tctttttcca aggcagcagc cgggccgtcg atattgcccg
     7681 cgccaaaaaa atcaccgccg cactgccgcc gtttgtcagc gttgtcgccc ttttcgtcaa
     7741 cgaaagcgcg caaaacatcc gccgcatcct tgccgaagtg ccgatacaca tcatccaatt
     7801 ccacggcgac gaagacgaca cattctgccg tcagttccac cgcccctata tcaaggccat
     7861 tcgtgttcag acgacatcag acatccgaaa cgccgccgac cgcttccccg acgctcaggc
     7921 actgcttttc gatgcctacc atccttcgga atacggcggc accggacacc gcttcgactg
     7981 gacgctgctg gcggaatatt cgggcaaacc gtgggtactt gccggcgggc tgacccctga
     8041 aaacgtcggc gaagccgtcc gcatcaccgg agcggaagcg gtcgatgtat ccggcggcgt
     8101 ggaagcatcg aaaggaaaaa aagatgccgc caaagttgcc gcctttatcg caaccgccaa
     8161 ccgcctattc cgttaaagca acaaaaattg ccgctggaat ga
//
LOCUS       DAWHRS010000090         8150 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWHRS010000090 DAWHRS010000000
VERSION     DAWHRS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.090"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            230..442
                     /locus_tag="WIB82_001423"
     CDS             230..442
                     /locus_tag="WIB82_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ3842711.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            590..2173
                     /locus_tag="WIB82_001424"
     CDS             590..2173
                     /locus_tag="WIB82_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ3842712.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICWNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2255..3352
                     /locus_tag="WIB82_001425"
     CDS             2255..3352
                     /locus_tag="WIB82_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ3842713.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            3466..4581
                     /locus_tag="WIB82_001426"
     CDS             3466..4581
                     /locus_tag="WIB82_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ3842714.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     VCGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4706..5161
                     /locus_tag="WIB82_001427"
     CDS             4706..5161
                     /locus_tag="WIB82_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ3842715.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5232..5708
                     /locus_tag="WIB82_001428"
     CDS             5232..5708
                     /locus_tag="WIB82_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ3842716.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            5753..6046
                     /locus_tag="WIB82_001429"
     CDS             5753..6046
                     /locus_tag="WIB82_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEZ3842717.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            6043..6315
                     /locus_tag="WIB82_001430"
     CDS             6043..6315
                     /locus_tag="WIB82_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEZ3842718.1"
                     /translation="MKMQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVV
                     FNLFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            6498..7415
                     /locus_tag="WIB82_001431"
     CDS             6498..7415
                     /locus_tag="WIB82_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ3842719.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKTKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLVASKTDLCT
                     PEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPAGRDFDGEKLTNFFNDLPNMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            7480..8037
                     /locus_tag="WIB82_001432"
     CDS             7480..8037
                     /locus_tag="WIB82_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3842720.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TAGTIAMARTTAPDSATSQFFINLADNASLDYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         2167 a         1951 c         2106 g         1926 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataaat gcgccgattg ccaaggcaat gatgctcaac
       61 cagggttttt gcgaagcgtt cattgttgtt gtcctgatgg gaattgccgc cattatgcgc
      121 aagcgtccgt attttttcaa accgccgcac cgtttccgcg tttgcccaag ttcacgcccc
      181 gacttacaat aacgcccgtc agaatgcagt accaacgaaa ggaaaaatca tggaaaccct
      241 tatcctcgac atcggcggca tgagctgcgg cggctgcgtc aaaagcgtta cccggatatt
      301 ggaaggcgta aaaggcgtgg caagcgtcga agtcagcctt gaaaacaaaa gtgcgaccgt
      361 cggatacgac cccgcgcaaa ccgatgcagg ggcgttgatt gaagccgttg aagacggcgg
      421 ctatgatgcc gcgttgaaat aaagcggcaa aaatgccgtc tgaagccttc gcgcctccca
      481 gatgccgtct gaagtacgct ccgcgccttc agatggcatt tgatttagtt gaacgggtag
      541 gatgaattgg ttaaacttgc gggcaatagt gccttcagac aaacaaaata tgccgaacaa
      601 gaagaaaaaa gccgcctttg cgcgtgctgc aacggagaac ggtgtgccgg acgtgtccaa
      661 catcaaaaat tcatatgatg atttgatgta cgaatccggc gcattctctc aaaccgccat
      721 caataatttg gaggcgcgcg cccgcctgat gggtttgcag cccgcgcccg ccgccaatgc
      781 caaggtcttg gagctgggct gttcgatggg cggaaacatc atcacgcagg cactttatta
      841 cccggatgcg gaatttgtcg gtatcgacct gtccggcagg caggttgcac agggcaacgc
      901 catcattgaa aaaatgggct tgaaaaatgt gcgtctggaa gaaaaagata ttttgaccat
      961 cgatgagtca ttcgggaagt ttgactatat catcgtccac ggcatttggt cgtgggtgcc
     1021 tgacgcagtt aaagacaaaa ttttttcgat ttgctggaac aacctgacca aacacggcat
     1081 tgcctatatt tcatacaatg tttaccccgg ctggaaacgg caggagcagt tgcgcgaaat
     1141 tatgtacttt gccggcaggg atgtgcttga agaacccttg gaagcgcgga cgcggaaagg
     1201 cttggacgcg ctcaaggcgc tggcggaaat tttggaaaac gacaagggct tggacggcgg
     1261 cggcaaactt ccggcgattc aaaaaatatt gaatcataat ttttattaca tcgcacacga
     1321 atatatggag gcgtttaacg acccgattta cgtcaacggc ttcatcgaat gggctaaccg
     1381 ccacaggctc gcatatatcg gggataccaa tttgcacgtg tcttttgttt cttggatggc
     1441 ggagcatacg cgggagcgga ttctggcatt ggcaggggac gattatattg ccaaagaatt
     1501 ttacagtgat attttatccg accgccaatt ccgccgttcg cttttatgcc gtgaggaagt
     1561 cggggatact gtcaggcgtg atgagtcggt tgccgtcgaa gtgatagaaa gtttgaattt
     1621 ccgcccggca agaggggaaa caatcaactt tgatgaaaac gatatcctgc tttcgggcat
     1681 acgcgatgtg atgaaaaccg gagaggcgtt taaaacggaa gatgttgcgg aaaatctcgc
     1741 ccgccgattt cccggtttgg aatttgaccg catgaaaatc aattcccagc ttttattgca
     1801 aaccattctc gggcgttttt ctgtttcatc agacaatgcg ggcaaaccat tttttgaaga
     1861 ccataaaacc tatgtgccgg cgcgctttac aaactatgcc gccgcctttg tggaacacgg
     1921 cgcggaagcg tttgtccggc ctgccaaccg ttacaacgaa agcaccccct cattcggata
     1981 cgggcatttg tacattatgc gccaattgtc gcggccgacg agcaaacagg cattgattga
     2041 aacggttgcc gaaaacttga acatcgtcag caccacgccc gacggtttga cattccatcc
     2101 gcctgccgaa gtgtatgtgg aagaaatatt ggcagacttg gcagacaggc attttctcgt
     2161 ttcggcggat tgacgggcaa gtcccggcgg caatccgtcc gatatatttt cagacaggaa
     2221 agacacacac tgcgaaaagc ggtgcacgac acatatgaac cagacattta ccttgcccga
     2281 tacgcgcccg tatccccaaa atccgattaa aaaccacctg ctgctcaatg cctaccagtt
     2341 ggcgcataat tcttcccagg cttcgcgcaa actctcgtcc ggccaacttc aaaccgaaat
     2401 caggggaatg cttgagcaaa accactatat caacctttcc ctcgcgctga cgatgtcgcc
     2461 cgatgccgga acttatgccg cgctgctttc cagtgtgaac gcggtactcg attgcgagaa
     2521 agaaggcgaa gtgcagtggt tcgccctgcc ggtcgtgctg gtgtccggct gcaaaaaaga
     2581 acgtgcaatc gagatgaagc tgccgacgga ggcattgttt gcctgcctgc aaaactatcc
     2641 gcacctgcgc gcgttgacgc aagagacgca atggctgcct tatcttgtgc attcttccga
     2701 tttgagcgcg gtcgcgccgg atgagtggtg gcgtgccaaa caaaataccg aagcggcggc
     2761 gcaacacttg cgccgtttcg ccccgcgccc tttgctgttg cccgaagggc agtccgtcca
     2821 cgtcgtttac gcgctgggtt tcggcagcgg caaagttcag acggcattgg gtcagaacct
     2881 tcttcaggca ggtctgcccc tgatgcaggt atggcaggaa aatcttgcat cggaaggcgt
     2941 tacgctgttt gccaatccgc tttccccgga ttctccggta cgcgcgcttt cagacggcag
     3001 ccacacgcgc caacgtatgg cgatggatgt gtttgcggca aacgcgatac gcgccgtccg
     3061 tatgcagagt ccgcgcgtcg gcgtggtcgc tgcggcaaag gcgggcggac agattttatt
     3121 cgggtttaat gcgaccgacg gcgcgtttga agtcgtgccg caggtgtttt cgtggcagct
     3181 ttcctttacc gacaacatcg ccgtcatcca gcaaaatttc ctcgacctga tggcggagtg
     3241 ccgcgtggaa cacgtttacc tgttgcacaa tcccttgggc gaacaggaaa gcatcccgag
     3301 ctatgcggaa gcattgaaac gggaagggca caatccgttt ttcagcgcat agtgatttgt
     3361 agtagtgata ggctttctca tttatataat aaatcaaaat aaagagcaca acactttttc
     3421 attctgtgtt gtgccttgag tgaaacgaaa ggatgaatta taaatatgaa gattgtaatt
     3481 gcaccggatt cgtttaagga aagcttgaca gctcaacagg tagctgaagc aataaaaaga
     3541 ggcttccaac aatcgatagc agatgtggaa tgtctcctct gtcctgtcgg tgatggggga
     3601 gaaggcactg tagatgctat ccgacattct cttgacctag aagaaaaatg tctccaagtg
     3661 acaggacctt ttggacaaaa agaagtcatg cgctattttc aaaaagaaca actagcccta
     3721 ttcgaagttg ctgatttggt tggtcttgga aaaatcccgc tagagaaacg aaatccatta
     3781 caaatccaaa ctcgtggtat tggagaattg attcgccacc tcattagtca agaaattaaa
     3841 gaaatctata ttggcgttgg cggtacggcc agtaatgatg gaggtattgg gattgctgct
     3901 ggtttaggtt atcaatttta tgatgaggat ggaaatgcct tacccgtttg cggtcaatcc
     3961 ttactaaacc tagcttctgt ttcaacagaa aatcgctatg aaattcctga agatgttcac
     4021 attcgtattt tagcagatgt tgtgagtccc ttatgtggac accaaggtgc gacctatacg
     4081 tttggcaagc aaaaagggtt ggattctact atgtttgagg ccgtagatca ggcaatacaa
     4141 gatttttatg aaaaagtctc ccctgcaaca ttaaaactta aaggagcagg agctggtgga
     4201 ggcatcgctg gtggtttgtg cgcctttgct caggcaagta tcgtatctgg aattgacacc
     4261 tgcctggact tgattgactt tgataagaaa gtgtcagatg ttgacttggt tatcgttggt
     4321 gaaggaagac tagatcgtca aagtttagca gggaaagcgc ctattggtgt agcaaaaaga
     4381 acccctgtcg gagttcctgt tgtcgctatt tgtggcagtc tcgttgaaga tttgccttcc
     4441 ctgccatttg aaaatatcca agctgccttt tctattttgg agaaaagtga acctttagaa
     4501 gatagtttga aaaatgccag cctctatttg gagcatacgg cttctaatat tggacactta
     4561 ttaaatatgc ctaagattta gccaaaccat ttcttccaga tggatgtttt ggctggttct
     4621 gccttatgcg gttacgatta accgaccgat gccgtctgaa agggtttccg cttcagacgg
     4681 catcgcgccg gaacagggag gggatatgaa aaaaccgaaa atcctttttg tctgcctcgg
     4741 caacatctgc cgttcgccga tggcggaata cattttgcgc cgccgcgccg ccgaagcggg
     4801 cattcccctt gaagcggaca gcgcggggac atcgggctgg cacgacggcg aagatatgca
     4861 ccgcgagacg gcaaagatat tgaaaaaaca cggtatcgat gcttcaggct ttaccagccg
     4921 caaaatccgc caaagcgatg cggcggcgtt tgactgcatc atcgcgatgg acggcaagaa
     4981 tttgtccgaa ttggaaagaa ccttcggcag gcggccggaa aaaatattca agctgaccga
     5041 cctgataccc gaaagcggtt acgaccatgt ccccgatccg tggtatacgg gcgattttga
     5101 agaaaccttc aggcttgcgg atgcgggctg tcgggcattg ttggaaaaga tttccaaata
     5161 aagcaattga atacaaatat aaaaccgcct cctgccgtat cggttgttca gacggcataa
     5221 acagagaatt tatgaaaaca aatttcaaac agaaaattat cgaacaggca cgcagcgaag
     5281 gcttgcaggt aaccgctttg cgcgagcagg tattagatat tgtcttgcag caaagcggcg
     5341 tgattaaagc ctacaacgtc ttgtcgcaga tgcagcagca aagcgagggc gtgcttgcac
     5401 cgcctaccgc ctaccgcgcc cttgattttt gggcggagca gggcgttttg cacaaagtgg
     5461 cggcggtcaa cggctatatt ttgtgcagcc acgcgcagca cgagtgcgac gaccattgcc
     5521 acgaccacga agaagccgaa gcgcaccaca gcgcgtttat tttggtctgc accgaatgtg
     5581 gcacggcgga cgagcaaacc ctgagccacg agtgggcggc actgcgcgca ggcgttgccg
     5641 aaagcggctt tgcgctgaaa gaagaacacg ttgttttaac cgggatttgt aaaaaatgtc
     5701 agcagtaatc ggtggtttgc attgataata agcctttcga aaggagcaga taatgaaacc
     5761 gtctcttttg ttgcaaagca gaaagaaaga aatattggca gttttcggga aatatccatt
     5821 gatttgcaat ccaagggtat tcggttcggt ttctcgcgga gatgatacgg aaaacagcga
     5881 cattgatttg ttggtggatg caaaaacagg gacaacatta ttggatttgg gcgggctgca
     5941 agaggaattg cagaaactct tgggcataaa ggtcgatttg ctgacacccg atgacatctc
     6001 ggcccacttt agagataagg tgttgactga ggctgtcgca ttatgaaaat gcagaaagaa
     6061 ttgtcggtct atttgaaaca gatattgcag gcagcgcaat atatccggct atataccgac
     6121 aaaatggatt atgggcaatt ttctgccgac acaaagactg tgcaggcagt cgtttttaat
     6181 ttgttcctta tcggtgaaaa cgcaacccat atccttaaat cgtatccgga atttgccgag
     6241 gaaaccaaat atttgaattg gatagggatg agggggtatt gcgcaatagg attgcccatg
     6301 gctactttga aatgaacctg tctgtcgttt gggagacggt tttgaacgtc ataccggcga
     6361 tgtcagccga tatatcgaat ttgctggaac aattgtcaat tgatgaagaa catgataaca
     6421 gctgccatca cttttaacat tatttaatca tttaaaacac cctatcttca aattttaaag
     6481 tgggacatca catagaaatg aaaaaaacca aagtccacct gatttcaggt tttctgggaa
     6541 caggcaaaac caccgcgctc aaaagcctga tggcgcaaaa agacccgaac gaaaaatggg
     6601 tcatcatcgt caacgagttc ggcgaaatcg gcattgacgg cgcggtattg agcgacaacg
     6661 gcatccctgt ggcagaaatc gccggcggct gtttgtgttg caccgccggc ccgcaaatgg
     6721 gcgtaaccgt gcagaaaatg ctgcgcgacg ccaagcccga ccgcctgatg attgaagcaa
     6781 gcggactggc gcacgccgcc agtgtcattg atgaactgaa aaccaaaccg ctggacagcc
     6841 ttttggaaat cggcgcagtc tttaccgtcg tcgatccgcg ccagttcatc aaccccgatt
     6901 acgcgcagca ggcattgtat aaagaccaaa tcggcatctg cgacgtattg gttgccagca
     6961 aaaccgattt atgcaccccc gaacagcttg ccgaatttca cgacaaagcc gcaaaactgt
     7021 tcccgcccaa ggctaaagtg gtcgaagttc aaaacgcaca actcgatatc caatggcttg
     7081 acatccccgt catcgaaaaa tcacgctacc gcctcaaagc cctgccggac aacacgatgg
     7141 gcttccagtc gcaaggtttc acattccccg ccggacgcga tttcgacggt gaaaaattga
     7201 ccaacttctt caatgatttg cccaatatga ccgaaggact cgtccgtgcc aaaggcgtgt
     7261 tccaagtatt gggaacgtgg gtgtggctca actgggtgga cgggcaatgg ggtgcgaacc
     7321 aagtgtcttg gcggcgcgac tcgcgttttg aattgattgc caaatcgttt gatgcggatt
     7381 taatcgaaca aaaactcaaa gacgcattgg aatagcgtta acgagccttc agacggcata
     7441 ggaaatgaaa atgccgtctg aaggtctgag gaggacggga tgaaacccaa attcaaaacc
     7501 gttttaaccg cgctgctttt ggcggtttcc ctgccgtcta tggcggcaac ccatgttttg
     7561 atggaaaccg atatgggcaa tatccgtttg gttttggacg aatccaaagc ccccaaaacc
     7621 gttgctaatt tcgtgcgcta tgcccgaaaa ggcttttacg acgacaccgt ttttcaccgc
     7681 gttatcgacg gttttgttat ccagggcggt ggattgaccg aggacttggc acaaaaggca
     7741 agcgataagg ccgttgccaa cgaatccggc aacggcttga aaaacaccgc cggcaccatc
     7801 gccatggcgc ggacgacagc ccccgattcc gccaccagcc aattctttat caatctggcg
     7861 gacaacgctt cgctcgacta caaaaacgga caatacggct ataccgtttt cggcagggtc
     7921 gaaagcggca tgaacaccgt ttccaaaatc gcccgcgtca aaaccgccac gcgcggcttt
     7981 tatcaaaacg tacccgtaca gcccgtcaaa atccgtcgcg ttgttgtcgg gcagtaacac
     8041 gcagacagac gttcagacgg cgtcgcccgt ttcccaaaaa acgccgttta aagtaaaaaa
     8101 atattttaaa acagacagtt gatattgaca aattcaaacc gaggatttta
//
LOCUS       DAWHRS010000091         8126 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWHRS010000091 DAWHRS010000000
VERSION     DAWHRS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.091"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(138..1271)
                     /locus_tag="WIB82_001433"
     CDS             complement(138..1271)
                     /locus_tag="WIB82_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842721.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGDKRGLSY"
     gene            complement(1391..1885)
                     /locus_tag="WIB82_001434"
     CDS             complement(1391..1885)
                     /locus_tag="WIB82_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842722.1"
                     /translation="MKNRFAKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWRELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEIFKMSVLEDWERSGKPLDWKKWQRQYRSEAFELVKKLNR
                     SKQE"
     gene            complement(2034..2369)
                     /locus_tag="WIB82_001435"
     CDS             complement(2034..2369)
                     /locus_tag="WIB82_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842723.1"
                     /translation="MMADKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLNIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKHP"
     gene            complement(2381..2740)
                     /locus_tag="WIB82_001436"
     CDS             complement(2381..2740)
                     /locus_tag="WIB82_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842724.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWIFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVNWKINNGNLIQTTN"
     gene            complement(2961..3416)
                     /locus_tag="WIB82_001437"
     CDS             complement(2961..3416)
                     /locus_tag="WIB82_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842725.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVEKNKIISIFCYQELYFMGVNIIGLNFEIFKQLFHYPKLNDID
                     KCYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSIEPYYDE"
     gene            complement(3397..3996)
                     /locus_tag="WIB82_001438"
     CDS             complement(3397..3996)
                     /locus_tag="WIB82_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ3842726.1"
                     /translation="MAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQETVYIEISDG
                     IGNNQTLISNRIHPFYSGGKWIKAEDLKAGSRLFAESGKTQTVRNIVVKPTPLKAYNL
                     TVADWHTYFVKGNQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQISAESKIAELTN
                     KIIPGMSKNERLKIKQKIKNIAKNANRKTKGEEHGRRGR"
     gene            complement(4162..4705)
                     /locus_tag="WIB82_001439"
                     /pseudo
     CDS             complement(4162..4705)
                     /locus_tag="WIB82_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
     gene            complement(4832..5059)
                     /locus_tag="WIB82_001440"
     CDS             complement(4832..5059)
                     /locus_tag="WIB82_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842727.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            complement(5118..5441)
                     /locus_tag="WIB82_001441"
     CDS             complement(5118..5441)
                     /locus_tag="WIB82_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ3842728.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQNEFGEMWTCYCSVNIVLSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            complement(5431..5784)
                     /locus_tag="WIB82_001442"
     CDS             complement(5431..5784)
                     /locus_tag="WIB82_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842729.1"
                     /translation="MGIKNEQIIICKKYNTEIYPISDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            complement(5768..6358)
                     /locus_tag="WIB82_001443"
     CDS             complement(5768..6358)
                     /locus_tag="WIB82_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842730.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDETSGVTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGKTQTVRNIIVKPTPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDC
                     PPYKRPNNATTKAQRESVQGKPCVEYGKLAEKMIADHKKPLVVEYYETGTIDKSKMRA
                     IESVQPQCPTCSAKQGGRLSQYSKEQKRKLSNGNKK"
     gene            complement(6494..7039)
                     /locus_tag="WIB82_001444"
     CDS             complement(6494..7039)
                     /locus_tag="WIB82_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ3842731.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYTGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRKPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNTVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMNRKPFVW"
     gene            complement(7036..>8126)
                     /gene="mafB"
                     /locus_tag="WIB82_001445"
     CDS             complement(7036..>8126)
                     /gene="mafB"
                     /locus_tag="WIB82_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ3842732.1"
                     /translation="GIGDILYGTRYAIDKAAMRNIAPLPAEGKFAAIGGLGSVAGFEK
                     NTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDFSKSYTCSF
                     HGSTLVKTTDGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQETVYIEVSDG
                     LGKIQTLVSNRIHPFYSDGKWIKAEDLKAGNRLLSESGKTQTVRNIVVKPTPLKAYNL
                     TVADWHTYFVKGDKAETEGVWVHNDCPYGSSGAKGKSGSERNTAKRGTFRQETLKNAW
                     DLAADGSQPNSKKCPTCGKDVYGNPHNKEKRNTKDGWDGSHNPSWSNRQHSSDRKEQL
                     NDYNKGVSLECRHCNRSGGNKDERFKKK"
BASE COUNT         2181 a         1824 c         1510 g         2611 t
ORIGIN      
        1 acccgtttaa tgtgtatttc cgttttttgg attgtggttt tcaatttgta gcgaatcaga
       61 ttcggtatat acggcattgc aaaaagcgtt tgactctcca atgccgtctg aaaaccggtt
      121 tcagacggca tttgcgttca gtaagaaagg ccgcgcttgt cgcccgaacg tctcgccgca
      181 gcctctgcat aacggctcac ctctttttcc aaattttcca agttcaaagg aaaatcaggc
      241 agtttgtctc cctgtttctc ttcgcggata atccgcccgc catccaaata ccacgtctgt
      301 tgcgcatgat aggtctgtat atccgccgtt acgccatccg ctttcaatgc taccgtcgaa
      361 gattgtgcaa taaaaagatt tccgtttttc aaataatatt cgaaactctg gcgttttttc
      421 ccattgtcga aactccaata gactttttgc ggcagaccgt ccgcatcata gccgaccaca
      481 agactgttcg ccttcatccc ccgtggcatc aattcccgca tattctgata aaacacagaa
      541 tcgcgcgagt ccgacgcaat ccggttgctc tcttcgcgga agtcccaaac cttctgctcg
      601 tcattcgcga catcccggta tttcgccaaa tatacctggg ccatctgata acacccgagg
      661 caatgctcat aaacatcttc cccgattttc ccgcgccccg ccgcatcaaa taccgaaccg
      721 tccggttgcc aaacaacccg atattctcct gtcgtttcat aattttcccc gtgagccgtt
      781 ccgccgtaca catttacaga aaacggacga tcgttccgat acagatattc ggcattaaca
      841 aacgcttccg gcgagcgttg cgaaagcgaa accgcaacca aaccgccctc gccgatatgg
      901 taatccagcc aaacctcttt cccatgttcc tgctccgtta cgtgaaacca tttcgccttt
      961 tctttcaaac gactgagccg gatagcgagc gcgagataat ccttctccga ctgcaacgga
     1021 ccgtcatcca cagctccggc aagactttcc tccgtcctca tcgattcctt cacgatgaca
     1081 accgccctgt cggcatttcg gaacaggcgg gcaagtttcg ccacaaaagc attcggattt
     1141 tcaggtattt cagttgctgt atcgctcaaa aaccaacgcg gattaatctc ataggcaata
     1201 cccgttccca gccaaaaggc aaatacaagt gcaaaaaatg acaacagtac cggtttgaat
     1261 tttttaaaca tatttatttt tcgtttaaca gaatatatcg attatatcag acgagctttg
     1321 attgtttgat ttttaattta atctacggtt gctgccgtac tttaaaaacc cttatgcagg
     1381 ctgcggcttg ttactcttgt ttgcttcggt tcaatttttt aactaattca aatgcttcag
     1441 atcgatattg cctttgccat tttttccaat ctaacggttt tcccgagcgt tcccaatctt
     1501 ctaaaaccga cattttgaaa atttccattt cccaccaata ttcggcatat aaacgtgatt
     1561 gattgggaat gtcggcttct gcccaatcgg caaaatgcaa aaaatgagaa atatcgcaaa
     1621 ctatattatt ttcaggaact tcttcggcaa atagatgctt gactgactgt aacgcttcgg
     1681 tctgatattt ttctgtccat ttctcccatt tttcaggctt tcccgatatt tcccaatctt
     1741 ccaatgcgga agcattcaag atttccaatt cccgccagca atcgtaataa cgagaggatt
     1801 ggttaggaat tttattttcc gcttcggaaa caaattgggt aaaactaaaa atatccttaa
     1861 cgatttttgc aaatctattt ttcataggtt gtcgccccct ccaaattgcg tgccaacaac
     1921 ttcggcaaaa tcaagccggc aagcccctgc atcctgtgcc aatcggagaa aatgccgtct
     1981 gaaagctgaa taccgcttca gacggcattt gccagatttg accttgccgg tgcttacgga
     2041 tgctttttaa atataaagaa ggaaatatcg tctaacgcat cttcggcaaa accttgaaaa
     2101 tccggattgc cttgttttga gcgcaataaa gcaatcactt tatcgcaatc gccgatattt
     2161 tcatgcatcc tagcgagatg tccgaaacag gttaatgaca atctggcaat attcaagtct
     2221 ttatgcttcg acaactccgt caatttctca gaaatccaag ggatttcatc ctcctctgaa
     2281 ctgaaggtca taccgagcat accgtcaata acactctgtt tattttcaga ataaatcatc
     2341 ttttctactt gttccttatc agccatcatt ttttgtccat ttaattagtc gtttgtatta
     2401 aattgccatt atttattttc caatttactt tataactatc ttcataataa tctaattcaa
     2461 aaaaacctga tatttcaata tccaattcca ttattgtttt aatacatttt tcaaaataaa
     2521 taatgaaata agattttacg catgcaccaa aaaaaatata gctgctccaa ttaaaactat
     2581 ttgtcgggaa aatccacccg cttttatata tttttgcaga ttctttctct tcgatattga
     2641 agggacaatt attccaaaaa ttattgatat tttcttggat gatttttata tcatcgtcag
     2701 agcattcaat ccatcctttt atggaaacat atgatgccat gtttaatctc ctaaacctgt
     2761 tttaacaatg ccgccttttg attcaatata tgacttaact tgtgaatgaa caccgtgcct
     2821 gttccgtaca gcaagcacag gttggattaa gattcgacaa tctgtttgga ttgacagaaa
     2881 ggtcgtctga aaaatcgatt acggtttcag acgacctttt gttgttgagt tttcaaccca
     2941 acctatgcct gccattaatg ttattcatcg tagtaaggtt ctatcgaata attgtctttg
     3001 cccccggcaa tgatagtaac aattttccct tttgcttccc aagcttgtac tcctatttca
     3061 tcaaactcat agacatatgt cggataagat tcattcgata aataacattt atcaatatcg
     3121 tttaacttgg ggtaatggaa aagttgttta aaaatttcaa aatttagacc tattatatta
     3181 acgcccataa aatataactc ctgataacaa aatatcgaaa taattttgtt tttttcgacg
     3241 gaaatgagta aatttgagtc gggagattca taatattctg ttccaaactc atcaggaggt
     3301 tcatacataa aagtttccag tatgtttttg tactgtgtta tatcagcacc aaaacggaat
     3361 attcctacag aagtaaaagg taaaaattcg ggagttttaa cgaccgcgtc gaccatgctc
     3421 ttctcctttt gtttttcgat tggcattttt ggcaatattt ttgatttttt gcttaatctt
     3481 taagcgttca tttttggaca ttccgggaat aattttattt gttaattcag caatttttga
     3541 ttccgctgat atttgacttc gaccgccatc tccatgtttt tcattcttgg agcttcctgt
     3601 tcttttaggc ggacaagaat tatgaaccca caccccttcc gtttccgcct gattgccctt
     3661 gacgaagtag gtatgccaat cggcaacggt cagattgtag gctttgagcg gtgttggttt
     3721 gacaacgatg ttgcggacgg tttgggtttt gccgctttcg gcaaacagcc tgcttcccgc
     3781 tttcaaatct tccgctttaa tccatttgcc gcccgaataa aacggatgga tgcggttgga
     3841 aatcagggtt tggttgttgc cgatgccgtc tgaaatttca atgtaaacgg tttcctggta
     3901 cggattgccg tatcgggcgg taacaggttt gtatccagtt tttccactgg tttcatcttt
     3961 ggcgaagacg cggtcgccgg cttgaatatg ggcaatggct ttgtagccgt ctgccgtttt
     4021 gactaaagca ccgacgtata agggacggag acaggatttt tgaagaactt agttcccaat
     4081 atcctgtttc aaatgaagga aaggtcgcct gaaaattaaa cacggtttca gacggcactt
     4141 tgattaaatt taagaaaatc cttaccaagt aaacggtttc cgattcatat ccgaaaggtt
     4201 gccaacttca ttatccaaca agaactgctc aaaagcattc caacctttct tttccaccaa
     4261 ttcggcttcc tgtttataca aggggacaag caaagggaaa acgatattgt agtgttcgcc
     4321 ataacagacc tgaaaatcat catcgaaata aaatggggcg gaaacataca gtgcatccat
     4381 ttttgttgaa tcaacaatgc ttctgggcaa ataaacaacc tgccccccga agaaggattt
     4441 ttttattctc aatgatggtt tctgccagcc atcttaaaaa tgggaaaatc tgattttcat
     4501 catattttaa agaacataca aaaagtattt caaatctgct tttataattc agatcatgtt
     4561 tgcttaaccc taaagtaata taagttttta aatccctgtt aggggaagag ggaatagctg
     4621 cgatatttaa tttgtactga cttcccgaat cgcttttcga gccatgagaa atagtgccta
     4681 tatatttttc aatatgatta atcatatttt tcttagcttc ccatcactgt atcgaataat
     4741 cataaaccgg attgagacaa aacgccttgt tcccattgat agaaagtccg tctgaaaagt
     4801 aaaagcgttt cagacggact acgtttgccg actatccgac ccactttatt atccaatgag
     4861 attcttgcag gtattctttt aatatttcta agttttcgac ccagtcatcg taactgtcaa
     4921 atgatgagaa tttgtcattt gaagataaca gtatcagaaa tcccccggta ttttcctgat
     4981 catcaataat ttttatgaat ttttcttcag gattgacccc attcaggata acccccaaag
     5041 tattgaattt tatgtccata gacacaccct aatctttaat gggaatatgg ttttttcaac
     5101 ccaacctaca ctttttgtta gttcccatac gaaccaaaac cttcataatt acaatcattt
     5161 ttttgtgcaa taccaaataa cgtagtttca atagctaaaa tgttttcaag ggaaaggacg
     5221 atattgacgg aacaatagca agtccacatt tctccgaatt cattttgctc aatactgatt
     5281 tgaaaatctt gccgataaga aatgagttgg ctttctattg attgcagatt tttttgagaa
     5341 cctcctataa aaaactctat tatcataggt tttgtcaaat caaaccctag ttcttgatat
     5401 tgtttcagta catctgaagt agatttttcg tcaattatca tttaatagca actcatcaaa
     5461 ccatacgtct tcataaccgt tttccccaat taaaaaacgg taaccgggcg gaagtgtcaa
     5521 aaaggggatg atttcgggtc gccatatttg caaatggaaa gtatgcaagg gcaggaaaaa
     5581 atttttatca taggagaagt tttcaccggc ccagatatac caaccgttag tatcaccttt
     5641 gggacggtgc cttaacccgt taatcggata caggtttgcc tgtttgacat tttcagcaac
     5701 accgattttt gaaacatcgg aaataggata tatttctgta ttatattttt tacaaattat
     5761 tatttgttca ttttttattc ccattactca gtttcctttt ctgttctttt gaatattggc
     5821 ttaatctccc cccttgtttt gcagaacaag tcggacattg cggttgtact gattcaattg
     5881 ctctcatctt agatttgtca attgtccctg tttcataata ctcgacaact aatggttttt
     5941 tatggtcggc aatcattttt tcggctaatt taccgtattc aacgcagggt tttccttgta
     6001 cactttctcg ttgtgctttt gtcgttgcgt tatttggtct cttatagggc ggacaatcat
     6061 tatgaaccca cactccttcc gtttccgcct gattgccctt gacaaagtaa gtatgccagt
     6121 cggcaacggt cagattgtag gctttgagcg gtgttggttt gacaatgatg ttgcggacag
     6181 tttgggtttt gccgatgccg tctgaaactt taatgtaaac ggtttcttgg tacggattgc
     6241 cgtatcgggc ggtaacgggt ttgtatcccg ttaccccgct tgtttcgtcc ttggcaaaga
     6301 cgcggtcgcc gactcggata cgggcgatgg ctttgtagcc gtctgccgtt ttgaccaagg
     6361 tgctgccgtg gaaggagcag gtgtaggatt tttgaagaac tcagttctca atattctgtt
     6421 tcaaatgaag gaaaggtcgt ctgaaaaaca agcttaattt cagacgacct ttaatcaaat
     6481 tcaagaaaat cctttaccaa acaaacggtt tcctattcat atccgaaagg ttgtcaactt
     6541 cattatccag caaaaactgc tcaaaagcat tccaaccctt tttttccacc aattccgctt
     6601 cctgtttata caacgggaca agcaaaggga aaacggtatt gtagtgttcg ccataacaga
     6661 cctgaaaatc atcatcgaaa taaaatgggg cggaaacata cagtgcatcc attttgctac
     6721 cttcgataat taccctgggt aattgaataa tttcacctct taaaataggt tttctattgt
     6781 caatcaaata ttcggctact cctgatagaa aagctgcaat attttcttta ttccattctt
     6841 cagaacaggt aaaaattaat tcaaacctac tcttatagtt caagtcatat cgattcaagc
     6901 ctaaagtaga aaaagtagtt acccctgtat atggttgtga tgggaatacg gaaacgctga
     6961 gatgataacg tcgatccaag cattttatac cgctttcgat ttcacccaaa taatgctcta
     7021 aatgttcaac tataatcatt ttttcttaaa cctttcatcc ttgttgccac cggatcgatt
     7081 gcaatgtctg cattctaaac ttactccttt attataatca tttagttgct ccttacgatc
     7141 agaactatgt tgacggttac tccatgatgg attatgactt ccatcccaac catcttttgt
     7201 attacgtttc tccttgttat ggggattacc atatacatct ttaccacatg taggacattt
     7261 ttttgaatta ggctggctac catctgctgc caaatcccat gcatttttta gcgtttcttg
     7321 tctgaaggtt ccacgtttag cagtatttct ctcacttcca cttttacctt tcgcgccaga
     7381 actaccatac ggacaatcat tatgaaccca aaccccttcc gtctccgcct tatcaccctt
     7441 gacgaagtaa gtatgccagt cggcaacggt cagattgtag gctttgagcg gtgttggttt
     7501 gacaacgatg ttgcggacag tttgggtttt gccgctttcg gataacagcc tgtttcccgc
     7561 tttcaaatct tccgctttaa tccattttcc gtccgaataa aatgggtgga tgcggttgga
     7621 aacaagggtt tgtattttac cgaggccgtc tgaaacttca atgtaaacgg tttcctggta
     7681 cggattgccg tatcgggcgg taacaggttt gtatcccgtt tttccactgg tttcatcttt
     7741 ggcgaagacg cggtcgccgg cttgaatatg ggcaatggct ttgtagccgt ctgtcgtttt
     7801 gaccaaggtg ctgccgtgga aggagcaggt gtaggatttt gagaaatccc cgctaaccgc
     7861 agctttcccc ggtttcgccg cctttgccag cttcgcgact ttggctgctg cggcaacgtt
     7921 gaagacggct tcgacggttt cggcggcatt ggggttttcc tgtatccacc ggtcaacggc
     7981 ttcacgcgta ttcttttcaa agcccgccac gctgcccaag ccgccgatgg cggcgaattt
     8041 gccctcggcg ggtaaggggg cgatgttgcg cattgcggct ttgtctatgg catagcgcgt
     8101 tccgtacagt atgtcgccta tgccca
//
LOCUS       DAWHRS010000092         7917 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWHRS010000092 DAWHRS010000000
VERSION     DAWHRS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.092"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            332..1645
                     /locus_tag="WIB82_001446"
     CDS             332..1645
                     /locus_tag="WIB82_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ3842733.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1863..2038
                     /locus_tag="WIB82_001447"
                     /pseudo
     CDS             1863..2038
                     /locus_tag="WIB82_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2034..3278
                     /locus_tag="WIB82_001448"
     CDS             2034..3278
                     /locus_tag="WIB82_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ3842734.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGPGLSIADTLAKKYGGHLEL
                     TDSRRFGHGLLIRALLDKETLK"
     gene            complement(3314..4807)
                     /gene="rng"
                     /locus_tag="WIB82_001449"
     CDS             complement(3314..4807)
                     /gene="rng"
                     /locus_tag="WIB82_001449"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ3842735.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            5020..5277
                     /gene="grxC"
                     /locus_tag="WIB82_001450"
     CDS             5020..5277
                     /gene="grxC"
                     /locus_tag="WIB82_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ3842736.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5298..5741
                     /gene="secB"
                     /locus_tag="WIB82_001451"
     CDS             5298..5741
                     /gene="secB"
                     /locus_tag="WIB82_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ3842737.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVEMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSSTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5827..7869
                     /gene="recG"
                     /locus_tag="WIB82_001452"
     CDS             5827..7869
                     /gene="recG"
                     /locus_tag="WIB82_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ3842738.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLVVQIADGSGSVLFLRFIHFYAGHQKQ
                     MAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGSAAALRGDGTLTQALRHALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDKLPPGRTPIKTRL
                     VNNVRRTEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLSELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1665 a         2054 c         2324 g         1874 t
ORIGIN      
        1 ccgccttgtc ctgatttttg ttaatccact ataaaatccc gcgtgtacgg cacgccccgc
       61 gctccgcaat gataggggcg gtttcgtttg aatcggcgca ccgggatgaa tgcccccgcg
      121 ccgtcccgat accggtttgc cggttgttcc gtatcggttg ccgccgtgaa atgacggacg
      181 gatacaggaa tggcggcagg cgtaaatggg aggaaatgac cgattgactt gtttcaaaat
      241 aaaaaagtag aatgcaaggc ttatcccgcg tctgtgggcg gttttgcctg cacggtcggt
      301 tttctgttta aaataaaata aaggagaaat gatgctgacg tttatcggac tgctgattat
      361 cggggtcatc gtatggttgc tgctgacgga aaaagtgtcg cccatcatcg cattaatctt
      421 ggtgccgctg tttggggcgt tgctggcggg gtttgatgta tcccaattaa aagaatttta
      481 ttcgggcggc accaaatcgg tgatgcagat tgtgattatg tttatgtttt ccattttgtt
      541 ttttggaatc atgaacgatg tggggctgtt ccgtccgatg ataggcggtt tgattaagct
      601 gactcggggt aatatcgtgg cagtgagtgt ggggacggtc ttggtgtcgg tggtggcgca
      661 gttggacggg gcgggtgcga cgacgttttt attggtcgtc cccgcccttt tgccgcttta
      721 caagcgtctg catatgaatc cttacctgct gtttttgctg ctaacttcca gtgcgggatt
      781 gattaacctt ctgccgtggg gcgggccgac tgggcgggtt gcaagcgtgt tgggcgcaga
      841 tgtgggcgaa ttgtataaac ctttgttgac ggtgcaaatt atcggtgtgg tgtttatcct
      901 tgcgctgtcc ctgcttttgg gtgtgcgtga aaaaaggcgg attgtccggg agttgggcgc
      961 gttgcccgcc gtggcggatt tgataaagcc ggtgcctttg tcggaagaag aacaaaaatt
     1021 ggcgcgtccg aaactgtttt ggtggaatgt cctgctgttt ttggcggcga tgagcctgct
     1081 tttttcgggc atcttcccgc cgggttatgt atttatgctg gctgcaacgg cggcgttgct
     1141 tttgaattac cgcagcccgc aggaacagat ggagcggatt tatgcccacg ccggcggcgc
     1201 ggtgatgatg gcgtccatta ttttggcggc aggtacgttt ttggggattt tgaagggtgc
     1261 ggggatgttg gacgcgattt ccaaagacat tgtgcatatc ctgccggacg cgctgctgcc
     1321 ttatctgcat attgccatcg gtgtgttggg cattccgctt gagttggttt tgagtacgga
     1381 cgcttattat ttcggactgt ttccgattgt ggagcagatt acctcgcagg cgggcgtggc
     1441 gcccgaagca gcaggttatg cgatgttgat cggcagtatc gtcggcactt ttgttacgcc
     1501 gctttcgccg gctttgtgga tgggcttggg tttggcgaaa ttgtcgatgg gcaaacacat
     1561 ccgttattcg tttttctggg cgtggggttt gtcgctggcg atattggcca gttcgatagc
     1621 ggcaggaatc gtgcctctgc cgtaaacggc gaagccgtct gaagcctgaa tcgttcctgt
     1681 ggtctgtcgc aggctctgcc tgcccgtgtg ttgcaatcgc gccccaatgc cgtctgaagg
     1741 cagttgccgg cgcggttttt tgtgccgcag ttttgaaaaa taccggcaag cgtaaaggcc
     1801 gctaaattgc ggctaatttt ggggtgttag aataggcacg gtttattttg aagggaaagt
     1861 tgatgcgtgt tttgctggtg gaagacgatg cgatgattgc ggaggcggtg tcggcaagtt
     1921 tgaaagacgg cggctatgcg gtggattggg tcaaaaacgg cgcgcggctg ccgctgccca
     1981 gccttatgac ttgatgctgc tggatttggg tttgctgggg cgggacggtt tggatgtttg
     2041 ttccgctggc aatgcttgca ggtatgtttt cctactacga aaccttccac gaaacggaag
     2101 cgttgcagga cgacctgctc cgtcaggcgg cattgtacgt cgcacccgat tccaaacccg
     2161 aaactttgcc cgagggcgac ggcgatacgc gcattttcgt gcagatgccg cagcaggaag
     2221 accctgttgt cagcctgcct gcgcatctgg cggacggtct gcacacgctt caggcggacg
     2281 gggacgacga ttattaccgc gtctatatcc gcacgaccga acagggacgg attgccgtta
     2341 tgcaggaaaa cgaataccgt gaagatttgg cggcggatgc ggcacggcaa agcgtgttgc
     2401 ccctgctggc ggcactgccg ctgatgatac tgctgaccgt gtggattacg cacaaagcca
     2461 tgcgccccgt ccgcaaattg tcgcaaagtc tcgaacaacg ccgaatcaat gacctgtctg
     2521 ctttgagtgt ggacaatatt cccagtgaaa tcagagggtt cgtaaccgcc atcaacctgc
     2581 ttttgaaacg tgcggatgaa gacatccgcc accgtcagcg gtttgtcgcc gacgcggcac
     2641 acgaattgcg tacgccgatg acagcccttt cccttcaggc ggaacggctc aacaatatgt
     2701 cgctcccacc cgatgcagcg cggcagcctg ccgttttgca gcagagcatc aggcgcaaca
     2761 aacacctgct cgaacagctt ttggcactgg cgcgttcgca gtcggatgaa acccctctga
     2821 cgaaaacgac attcggtctg caaagccgtt tccgccaagt gttgcaggaa ctgatgccgc
     2881 ttgccttgga aaaacgtcag gacattggtg tggcggtcgg aggtgatgtc gaaatatttg
     2941 ccgatgaaac tgaaatctat accttggtta aaacgtttgt tgataacgca atacgttata
     3001 ccccgtccga aggcaggata gatttgggtt tcacagacga agggaaatat cttgccgtgt
     3061 gggtggaaga taacgggaac ggcattccgg aatccgaacg cgcccgcgtc ctcgatccgt
     3121 tttaccgtat tttgggaacg gagcagcagg ggccggggct gtccatcgcc gacacgcttg
     3181 ccaaaaaata cggcggacat ttggaattga ccgacagccg acgttttgga cacggtctgt
     3241 tgatacgggc gttgttggac aaggaaaccc tgaaatagac agatgccgtc tgaagccttc
     3301 agacggcatt tttttacatc agcactatat cgtattgttc ctgtgtgtaa gcggtttcga
     3361 ccgccagaga aatcggtttg ccgatgaaat ctatcagcat tgccaaggat tgcgattctt
     3421 cgtccaaaaa caaatcgatg acgttggggg cggcgaggat gcggaaactt tcggcatcgt
     3481 aacggcgcgc ttcgcggacg atttcgcgct ggatttcgta gcatacggtt tgcggcgttt
     3541 tcagacggcc tctgccttgg caggaagggc agggttcgca gaggacttgg tttaagtttt
     3601 cgcgcgagcg tttgcgcgtc agctcgacca gccctaggct ggtaaaaccg tgcagggtaa
     3661 cgcgggtacg gtcgaaggcg agggctttgg caagctcctg caacacggct tcgcggtggc
     3721 tttcctgtgc catatcgatg aagtcgatga tgatgatgcc gccgaggttg cgtagcctca
     3781 attcgcgggc gatggtgtgg caggcttcta agttggtgcg gaagatggtt tcgtcgaagt
     3841 tgcgtgcacc gacaaagccg ccggtgttca cgtctatcgt ggtcatggct tcggtggatt
     3901 cgataatcag gtagctgccg aagttgaggt tgacgcgcgg ttgcagggcg cggctgattt
     3961 cctgctcgac gttgtgggtt tcaaacagcg ggcgttcgcc tttgaacaat tctatcctgc
     4021 ccaatgcgcc gtggacgtat tgttcggcaa aacgcgtcat ccgtccgtgg tttacggtgg
     4081 aatcgacgag gattttctgc gtgtcgcagc cgaccatatc gcgcaacacg cgcaggctta
     4141 aaggcaaatc ctgataaagc agggtttcgg gcggccggat tttcgcctgt tcttggatgt
     4201 gttcccacac tttggtcagg tagtcgatgt cggactggag ctgttcgtcg gtggcgtttt
     4261 cggcgttggt gcggatgatg tagcctcggc aggcattttc cggcaggagc ttgtcgaggc
     4321 gttcgcgcag gctgctgcgt tcggcatcgt cttcgatgcg ttgggacacg ccgatgtggt
     4381 cttcttgcgg aagatggacg aggaaacgcc ccgccagcga gatttgggtg gaaagccgcg
     4441 cgcctttggt gttgatcggg tctttgatga cctgcaccaa aacagactgc ccttcaaaca
     4501 gcatatgttc gatgcgctgg gtttcttcgg ggttgcggcg ttgttcgagg acatcgacga
     4561 tgtgtaaaaa cgccgcgcgt tccaagccga tgtcgataaa cgcgctctgc atcccaggca
     4621 gcacgcggcg caccacgccc agatagatat tgccgaccag gctgtgttcg ctgttgcgct
     4681 cgatgtgcag ctcgcagata ttgttttcct ccaacaccgc tacgcgcgtt tcctgcggcg
     4741 tgatgttgac caatatcgtt tcgggcgggc gcgcgatgtc tttggggatg gggagtcctg
     4801 acaacatggt ttttcctgaa aatgtaataa aaatattttg catccgttcc gccgcgcagc
     4861 gcgggtgcaa aaatattttt cacaatgtct atcatacttt aaaaaagaaa ctttgacaca
     4921 ttccgcacat gccgtttgaa cgtgcatctg ttccgcactt gccaaacagg ggaaatgccc
     4981 ctatattgat tatcattgca aaactttcgg aaacccaata tgcagaccgt taccatgtac
     5041 acaggtccgt tttgtcccta ctgcgcgatg gcgaaaaggc tgctgcacgc ggcaggtgtc
     5101 ggacatatcg acgaaatccg tgtcgatgca agccccgaag cctttgccga aatgcagcag
     5161 ctttcgggac agcgcagcgt gccgcagatt ttcatcggcg aaacgcacgt cggcggattt
     5221 accgacctct accgcctcca gcaggaaggc gggctggacg gactgctgaa cccttaaccc
     5281 ccaactagga aaacaaaatg agcgaagaac tgcaacccgt attcagcatc gagcgactgt
     5341 atgtcaaaga cttgtctttg gaagtgccgc acgcgccgca aatctttttg gaacagggcg
     5401 agcccgaagt ggagatgcgc gtttccaccg gcagccaaaa gctggaagac ggctactaca
     5461 acgtggatgt aaccgttacc gtaaccgcca aattggataa cgagcgcacg atgtttttga
     5521 acgaagtaac ccaaagcggc attttccgtt tggaaaacat ccccgaagaa gatgtgcagc
     5581 tgctgttggg cgtggcgtgt ccgaacatcc tcttccctta cgcgcgcgaa gccgtttcca
     5641 gtacggtaac gcgcgccggc ttcccgcccg tcctgctcgc gccgattaat tttgaggcga
     5701 tttaccaaca acagcaggaa gccgaagccg ccggggcttg attcctgcct gtcgatgccg
     5761 tctgaatccg tttcagacgg catttttgtt ttcggataaa ataggcgcgt ccaaaggaat
     5821 ctgcctatga tgtcgcccga aacccgaaaa cagctcaaaa tcaccgatgt ttccgccaag
     5881 aagctcgaca aactcaacct tcataccgcg tgggatttgg ttttgcatct gccgctgcgt
     5941 tacgaggacg agacgcacat tatgccgatt aaggacgcgc cgatcggcgt gccgtgtcag
     6001 gtcgaggggg aggttatcca tcaggaagta acgttcaaac cgcgcaagca acttgtcgtc
     6061 caaatcgccg acggctccgg cagcgtcctt tttctgcgct tcatccactt ttacgccggc
     6121 catcaaaaac aaatggcggt cggcaaacgc atccgcgccg tgggcgaaat caaacacggc
     6181 ttttacggcg acgagatgat tcatcccaaa atccgcgatg ccgagggcgg cggtttggcg
     6241 gaaagcctca cgccggttta cccgaccgta aacggtttga accagcccac tttgcgccgt
     6301 attattcaga cggcgttgga cgttacgccg ctgcacgaca cgctgcccga tgccctgctg
     6361 tgccgtctga agctgccaca cctcgccgaa agcctgcgtc ttttgcattc gccgccgccg
     6421 agtttcacga ttcatcagct ttcagacggc acgttgcctg catggcaacg gctcaaattc
     6481 gacgaacttt tggcgcaaca gctttcgatg cggctggcgc ggcagaagcg tatcggcggc
     6541 tcggcggcgg cattgcgcgg cgacggcacg ttgacccaag ccctgcgtca cgctttgccg
     6601 tttgccctga ccgatgcaca agaaaaagtt gtttccgaaa tctgccgcga tatggcgcaa
     6661 acctacccga tgcaccgcct gctgcagggc gatgtcggca gcggcaaaac cattgtcgcc
     6721 gccttgtccg cgctgaccgc catcgaatcc ggtgcgcagg tggctgtaat ggcgcccact
     6781 gaaatccttg ccgaacagca ttttattaaa tttaaacaat ggctcgaacc tttgggcatt
     6841 gaagttgtcc gccttttcgg cagtttgcgt aaaaaagcca aagacgaagc caaagccaaa
     6901 ctggctgacg gcagcgtcaa aatcgccgtc ggcacgcacg ccctgttttc agacggcgtg
     6961 gcgtttcaca atttaggctt gaccattgtg gacgaacagc accgtttcgg cgttgcccaa
     7021 cgcctcgccc tcaaaaacaa agggcgcgaa gtccatcagc tgatgatgtc cgccacgccc
     7081 atcccgcgca cgctcgccat gagttttttc gccgatttgg acgtgtccgt catcgacaaa
     7141 ctgccgcccg ggcgcacccc gattaaaacg cgcctcgtca acaacgtccg ccgcaccgaa
     7201 gtcgaaggct tcgtcctcgg cacttgccga aaagggcggc aggcgtattg ggtctgccca
     7261 ttgattgaag aaagcgaaac cctgcaactg caaaccgccg ccgaaaccct cgcccggctt
     7321 cagacggcat tgcccgaact caatatcgga ctggtacacg ggcgcatgaa ggccgccgaa
     7381 aaggccgaag tgatggcgcg gttttcttcg ggggggctga acgtcttggt cgccaccacc
     7441 gtcatcgaag tcggcgtaga tgtgcccaac gccgccctga tggtgatcga acacgccgag
     7501 cgcatgggct tggcgcagct gcaccaatta cgcggtcggg tagggcgcgg cgcggcagaa
     7561 agcgtgtgcg ttctcctgtt tgccgagcca ttgagcgaac tcgccaaagc gcggttgaaa
     7621 gtcatctacg aacacaccga cggctttgaa atcgcccgcc aagacctcaa catccgcggc
     7681 cccggcgaat ttctcggcgc gcgtcaaagc ggcgtgccta tgctgcgttt cgccaagctc
     7741 gaagaagact tacacctttt ggaacaagcg cgcgaaatcg ccccgatact gattgagcaa
     7801 aaccccgaaa tcgtcgaagc gcatttggca agatggcttt ccggcaggga agggtatttg
     7861 ggcgtgtgag caaaatgccg tctgaaatat tagaaaaaaa agtgtaacgg aaatctg
//
LOCUS       DAWHRS010000093         7889 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWHRS010000093 DAWHRS010000000
VERSION     DAWHRS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.093"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..140)
                     /locus_tag="WIB82_001453"
     CDS             complement(<1..140)
                     /locus_tag="WIB82_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ3842739.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LWT"
     gene            382..1536
                     /gene="obgE"
                     /locus_tag="WIB82_001454"
     CDS             382..1536
                     /gene="obgE"
                     /locus_tag="WIB82_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ3842740.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAACPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLIEKKRIEAEKAEAERAATNVEIAEQQPKTDTGVFKPE"
     gene            1619..2668
                     /locus_tag="WIB82_001455"
     CDS             1619..2668
                     /locus_tag="WIB82_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3842741.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3356..4777
                     /locus_tag="WIB82_001456"
     CDS             3356..4777
                     /locus_tag="WIB82_001456"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ3842742.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAAFDLSENANDYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4860..5639
                     /gene="xth"
                     /locus_tag="WIB82_001457"
     CDS             4860..5639
                     /gene="xth"
                     /locus_tag="WIB82_001457"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ3842743.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5626..5970
                     /locus_tag="WIB82_001458"
     CDS             5626..5970
                     /locus_tag="WIB82_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842744.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICMPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            5980..6510
                     /locus_tag="WIB82_001459"
     CDS             5980..6510
                     /locus_tag="WIB82_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ3842745.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6739..7854)
                     /gene="asd"
                     /locus_tag="WIB82_001460"
     CDS             complement(6739..7854)
                     /gene="asd"
                     /locus_tag="WIB82_001460"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ3842746.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGAETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2069 a         1983 c         2033 g         1804 t
ORIGIN      
        1 gtccacagga caatggtggt cagaatgccg atgaagccgg caacggtcag ccagtgcagc
       61 gcgcgtgtca acgggtcgta aacggcaggt tggtttgtgt tcataaaaaa cttattatat
      121 gtaacggttt gaacggacat tccgtcgggt cggaatgtca aaaaggcgcg attgtaccaa
      181 agaagttgga cataattttg tttgcaggct gaagatttgc ttaaaaattc attaagatgg
      241 gcggaacaaa tagtttgggt acggcgtgtt gaaataccgc ttatccctta taatagcgtc
      301 cgaaattctg ttcggacggc atcaaaacac acggtaatcc gtctgaaccc cccatttgaa
      361 accaacaaac aagagcattg aatgaaattc atcgacgaag caaaaatcga agtcgccgca
      421 ggcaaaggcg gtaatggcgc aaccagtttc cgccgcgaaa aattcgtacc gcgcggcggc
      481 ccggacggcg gcgacggcgg caaaggcggt agcgtctggg cggaagccga cgaaaacacc
      541 aacaccctcg tcgaataccg cttcgttaaa cgctaccaag ccaaaaacgg cgaaaaaggc
      601 cacggttccg accgttacgg cgcaggtgcg gacgacatcg tcctcaaaat gcccgtcggt
      661 accctcatcc gcgacctcga caccgacgaa atcgttgccg acctcactta ccacggacag
      721 cgcgtctgcc tagccaaagg cggcaaaggc ggcttgggca acatccactt caaatcctcc
      781 gtcaaccgcg ccccaaaaca atccacgccc ggcgaagaag gcgaaacccg ttccctgcaa
      841 ctcgaactca aagtcctcgc cgatgtcggc ctgttaggca tgcccaacgc cggcaaatcc
      901 accctgatta ccgccgtctc cgccgcatgc cccaaaatcg ccaactaccc ctttaccacc
      961 ctgcatccaa acttaggcgt ggtacgcatc gacgaaaacc acagcttcgt gatggccgac
     1021 atccccggcc tgattgaagg cgcggcagaa ggcgcaggcc tcggccatcg tttcctcaaa
     1081 cacttatcac gtaccggcct gctgctgcac gtcgtcgatt tggccccgtt tgacgaaacc
     1141 gtcaaccccg ccgaagaagc cctcgccatc atcaacgaat tgcgcaaata cgacgaagaa
     1201 ctctacggca aaccacgctg gctggtgctg aacaaactcg acatgctcga tgaagaagaa
     1261 gcccgggcgc gaacagccgc cttcctcgaa gccgtcggct gggactaccc gaaaccagac
     1321 gaccgcttcc aattcgacat ggaaaccccg cgcctcttcc aaatcagcgc gctgacccac
     1381 caaggcacgc aggaattggt acaccaaatc aaccaatacc tgatcgagaa aaaacgtatc
     1441 gaagctgaaa aagcggaggc ggagcgggca gcgacaaatg ttgagattgc cgagcaacag
     1501 cctaaaacgg atacgggcgt gtttaagccg gagtgaaaaa agaaagggtt tcagacgata
     1561 tttgttctac aaaaggttgt ttgaaacaat tttaaagcaa gattgaaatg gattaaaaat
     1621 gtcatctaac caatatatcc agtcgcttga gttgacaaac tttaccgttc ttccaaacga
     1681 tacttttgaa ttttctgaaa atctcaatgt tattgttgcc gaaaacggtt gtggcaaaac
     1741 acatttgtta aaaattttat acagcctgct agaagtaaca tctaacacca aaaacagatt
     1801 actgaaaaca gaattgcaaa aaagttttgc cgataaatta ctgaatgttt tccgtcccga
     1861 ttccttgggc aggttgagca aacgtttgca ggggcgcggg cgtacggaaa tcgttttaaa
     1921 actgcaaaac ggcactacgc atagtagtct gaatttttcc agtaactcat catcgcaagt
     1981 caatgttcaa agcatcggat tgaaagaaaa tgaatatact ccgacaccta tcttcttacc
     2041 cagccgcgaa ttaattacac tttgcccttg gttcacttct ttgtatcaaa accaaagcat
     2101 ccctttcgaa gaaacttggt ttgatacctg tatgcaatta aatcacccgc ttgccaaagg
     2161 ccccagagaa acaaaaattc gagaattgct cgaacctatt gagaacgcga tgggcggaaa
     2221 agtatctgaa gaacaaggaa ggttttatct atcgctgaca aatacgggcg gtaagattga
     2281 agcacctttg gtagcagagg gtttgcggaa atttgtcatg attgcccggt taatcgcaac
     2341 aggggcattg ctggataaag gctatctatt ttgggatgaa ccggaagcta atctgaatcc
     2401 taaattgatc aaagtggctg cccgtattat ttggtcttta tctcaacagg gtattcaggt
     2461 ttttattgca acacactctc tattcttatt gagagaattg gaacttttga agcttgaaaa
     2521 taacacggat ttacctgtac gttttttctc gttgattgct tccgatgatg gaacaaaagt
     2581 ggagcaaggg aacagtatta atgatttgaa cacattgata ttgttagatg aaaacattat
     2641 gcaggcagac aggtatctga atatttgagg ttaagctatg actacaatca cagaaaagag
     2701 attaaccttt gcttttcctg aagattatta cgttaccaag tatgatgaat gggaacatta
     2761 taaaattttt cagaacagtt gtaatttacg taataaaatt gatacaaatg aaaagggaaa
     2821 gaacgggata aatcaaagtg tagatgacga taatggcagc tcaggtgtag atataatagc
     2881 ccttcatgaa tcgacacttt ggctaatcga aattaaagat tattaccgac ttgggcttga
     2941 gccaaatgcc caatcaatag atgaaaaact ttctgaccta ccatatttaa tcgcaagaaa
     3001 aattcgagat tcattggcag gattggtttc tgctaaattt aaagcagaaa aacaggaaga
     3061 aaaagatttt tctcgcttgg ctttagattg taacgaaata aaaatagtcc ttcatattga
     3121 aatgcctagt ataagaagta aactttatcc ctcgtcatca gatttagcaa acttattaaa
     3181 agataagttt aaattatcag aatttacaaa aaattttgca aactgttatg cagaaccaat
     3241 tttcacaaat atcagccata ttaataatcc gcaactacgt aatgttcctt ggtccgtatc
     3301 tacaggaaca gaacagaaat tatcatccga gcaacaaaga tcaatccata gtcccatgac
     3361 caccatctac aacaccctta cccgccaaaa agaacccttt gcccccatcg accctgaaaa
     3421 cgtgcgtatg tacgtttgcg gcatgactgt ttacgactat tgccacttag gccatgcccg
     3481 tgtgatggtt gtgttcgaca tgattgcccg ttggttgcgc gagtgcggct atccgctcac
     3541 ttatgtacgc aacatcaccg acatcgacga taaaatcatt gcccgcgcgg ctgaaaacgg
     3601 cgagaccatc ggcgagctga ctgcgcgttt cattcaggct atgcacgaag atgccgatgc
     3661 tttgggtgtg ttgcgtcccg atatcgagcc gaaggcgacg gaaaacatcc cacaaatgat
     3721 tgccatgatt gaaaccctga ttcaaaacgg caaggcatat cctgccgcaa atggcgacgt
     3781 ttactacgcc gtgcgcgagt ttgccgctta cggccaattg tcgggcaaat cgttggacga
     3841 cctgcgcgcg ggcgaacgtg tggaagtgga cggtttcaaa cgcgatccgc ttgattttgt
     3901 attgtggaaa gcggcgaaag caggcgagcc ggcatgggaa agcccgtggg gcaacggccg
     3961 tccgggttgg cacattgaat gctctgccat gagcgaaaac ctgttcggcg ataccttcga
     4021 catccacggc ggcggcgcgg atttgcagtt ccctcatcat gaaaatgaaa tcgcccaaag
     4081 cgtcggcgcg acggggcata cctgcggtca tcatcacgcg caaacccacc acgggcaaag
     4141 catcgccagc cacgtcaaat attggctgca caacggcttt atccgcgtgg acggcgaaaa
     4201 aatgtccaaa tcactgggca acttcttcac catccgcgaa gtgttgaaac aatacgaccc
     4261 cgaagtcgtg cgcttcttca tcctgcgcgc ccactaccgc agcccgttga actattccga
     4321 cgcgcatttg gacgatgcaa aaggtgcgtt gacccgtcta tacaccacct tgaaaaacac
     4381 gcctgccgcc gcgtttgatt tgtctgaaaa cgccaacgac tacacccgcc gcttctacgc
     4441 cgccatgaat gacgatttcg gcacggttga agccgttgcc gtgctgttcg aactggcagg
     4501 cgaagtgaac aaaaccaatg atgcgcacct cgccggctgc ctgaaagcct tgggtggcat
     4561 catcggactg ctgcaacgcg atccgaccga gttcctgcaa ggcggcgcgg cttcagacgg
     4621 cctctccaac gaagaaatcg aagacttaat cgcacggcgc aaacaagcgc gcgcggataa
     4681 aaactgggca gagtccgacc gcatccgcga ccttctgaac gaacacaaaa tcattctgga
     4741 agacaacgcc ggcggtacga cttggcggcg cggttaaaat ccatcgaaaa gggtaaatgt
     4801 gccgcatttt gccctttttt gttcagacgg catttttatt gtgagaactt atttttatta
     4861 tgcttaaaat catttccgcc aacgtcaacg gcatccgctc cgcctacaaa aaaggttttt
     4921 acgaatacat cgccgcatcg ggcgcggaca ttgtctgcgt gcaggaactc aaagcgcagg
     4981 aggctgattt gtctgccgat atgaaaaatc cgcatgggat gcacggctat tggcattgcg
     5041 ccgagaagcg cggttacagc ggcgtggcgg tgtacagcaa acgcaaaccc gacaatgtgc
     5101 aaatcggtat gggcattgaa gaattcgaca gggaagggcg gtttgtgcgt tgcgatttcg
     5161 acaggttgag cgttatttcg ctttacttgc ccagcggcag cagcgcggaa gaacgccagc
     5221 aggtgaaata ccgtttcctt gatgcgtttt accctatgct cgaagcgatg aaaaacgaag
     5281 gacgcgacat tgtcgtttgc ggcgactgga acatcgccca ccaaaacatc gacctgaaaa
     5341 actggaaagg caatcagaaa aattcgggct tcctaccgga agaacgcgag tggataggca
     5401 aggttatcca caagctcggt tggacggata tgtggcgcac gctttatccc gatgtgccgg
     5461 gctacacgtg gtggagcaac cgcgggcagg cgtatgcgaa agatgtcggg tggcgcatcg
     5521 attatcagat ggttacgccc gaacttgccg ccaaagccgt gtccgcacac gtttataaag
     5581 atgaaaaatt ttccgaccat gcgccgctgg tcatggagta tgactatgct gccgaataaa
     5641 gttttgggta aatatgattg gaatgtggac ggaaaaacag gaataggcgc agcatgggtt
     5701 gtcgctgcgt ttatcctgcc catattggtg tgggcgatat ttatgttgtc gcggatgcaa
     5761 ggctggcttg cgccgaccaa ggcaaacccg atatgggcgt tggtgtggct gctgatctgc
     5821 atgccctgcc tgctgattgc gtccaaatgt ttgggttgga aaggctggcg gctgttggca
     5881 aacgttcttg cctgcctcgc cgtgtgcgct ctgttgagtg tgcctgccgc gctgctgatt
     5941 gcgttcacgc tgcaagacct gctcaaatag gcgcggacaa tggaaagttt cgagcttgag
     6001 gatttgacgc tgtggctggt acgcgatgcg gatgaagcgg aaatgtggat agaccgctgg
     6061 gcgatcagtt atcccgtcgt gcagatgtcc gaagcgtcgg ccggtcaaag cataggggaa
     6121 tggcaggcag ggcttcagac ggcatttgaa cgcatacgcg gcaaatacgt cgccgttgtc
     6181 gcacacggtg cgggcgcggc cgcatttttg gcgtggctgt atcaggcaga catcctgaca
     6241 cggaagaaaa ttgccaacat catccttgta ccgcagcgtc ccgatatttt tcccgacgat
     6301 gcggaacaca ctttccaacg cgtccgctgt ccctgccgtg ccgcattggt tgtacccgaa
     6361 cacggcggcg tgccgcacgg ttgggcgcaa aaacaggcgg atttgtggaa cgcccgcctg
     6421 ttggtttccc cgcattcggg cagtttgaac ggtatgctcg gcggctggca gtggggtatg
     6481 aagctgatgc aggaaatgtt gctggcgtga acgcttgcct tatcggaatt tcggctgtag
     6541 caggaagtcc gcatgtcggg ctgttttcat tgacgtgcag gcgcatcagg ttcggataaa
     6601 gtcgggctgc cgcataccgc cggaaatgga atcctttatt ttttacgtcg gcagtgccgg
     6661 cagggcgtta tccggcaggg ttgcaaggaa aatgccgtct gaaacaggct tcagacggca
     6721 tttccattca aacagggctc acaggctgcc caacacgata cgcaatacgc ggcgcagcgg
     6781 ttcggccgcg ccccacaaga gctggtcgcc gacggtgaac gcgctgatgt attcgccgcc
     6841 catgcccagt ttgcggatgc gtccgacagg gacggacagc gtgccggtaa ctttggcagg
     6901 agtcagctcg cggatgctgg cttctttttc attggggatg actttcaccc agtcattcgc
     6961 gcctgccaaa atcgtttcga tttcggaaac aggcaggtct tttttcaact tcagagtgat
     7021 ggcttggctg tggcagcgca tcgcgccgac gcggacgcac aggccgtcaa tcacggttgg
     7081 attgtcgctg cggccgagga ttttgttggt ttccgcgccg cctttccatt cttctttgga
     7141 ctggccgttg cccaaatcca cgtcaatcca cggaatcagg ctgccggcga gcggtacgcc
     7201 gaagttggct ttcggatagt cttcgctgcg caggaaatcg gatactttgc ggtcgatgtc
     7261 gagaatcgag ccggcaggat cggcaagttc gtccgccact tgggcgtgaa ccgcgcccat
     7321 accgctgatg agttcgcgca tgtttttcgc acctgcgccg gaagcggctt ggtaggtcat
     7381 gctggttgcc cattcgacca aatcgttttg gaacaggccg cccaaagcca tcagcatcag
     7441 ggagacggtg cagttgccgc cgatgtagtt tttcacgccg tttttgaggc cgttgtcgat
     7501 aacgttgcgg ttgacagggt cgaggacgat aatcgcgtcg tctttcatgc gcagagaaga
     7561 tgccgcgtca acccagtagc cgttccagcc gctgtcgcgc agggcttgga acacggattt
     7621 ggtgtaatcg ccgccctgac aggtaacgat gatgtccatt tttgccaatt cggcaacatc
     7681 gttggcatcc aacaatgttt tggctgcctg accgaaatca ggggctgcgc cgccgacgtt
     7741 ggaagtggta aagaaaaacg cttcaggaat gtgggcgaag tcgttttctt ctttcatacg
     7801 ctgcatcaaa accgaaccga ccataccgcg ccagccgacg aaacctactt tcattgtgtt
     7861 gctccttgag gaaaaaatcg ttaatatag
//
LOCUS       DAWHRS010000094         7888 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWHRS010000094 DAWHRS010000000
VERSION     DAWHRS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.094"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            370..594
                     /locus_tag="WIB82_001461"
     CDS             370..594
                     /locus_tag="WIB82_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3842747.1"
                     /translation="MKVISVLNQKGGSGKTTVATHLARALQLAGNSILLVDSDPQGSS
                     RDWAAVHEEQPVPVIGIDSPTIDRDLKSVV"
     gene            607..1014
                     /locus_tag="WIB82_001462"
     CDS             607..1014
                     /locus_tag="WIB82_001462"
                     /inference="COORDINATES: protein motif:HMM:NF025001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEZ3842748.1"
                     /translation="MIDGAPQTADLAISAIKASDLVLIPVQPSPYDIWATADLVNLIK
                     QRIKLIDGNLKAAFLINRTIKGTKIGGEIRAALEEYELPVLSTVIVQRIIYPTSAITG
                     STVLDKEPNGEAAKEIQSLANEIINLINIDNHI"
     gene            1057..1260
                     /locus_tag="WIB82_001463"
     CDS             1057..1260
                     /locus_tag="WIB82_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEZ3842749.1"
                     /translation="MNGLKADRPSANKANLSIDDVIKQEEIKRISFDMSESLHRELKK
                     YAAENGVTIRVVLNKLVSDLLNK"
     gene            1436..2599
                     /locus_tag="WIB82_001464"
     CDS             1436..2599
                     /locus_tag="WIB82_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ3842750.1"
                     /translation="MHINFSMEYKEFNENYLYKKPFIFKNALDVSSISWKEINELYQR
                     ADPTDWQFKFRKGEIIPKEAYVESFNDVGRIRHRFNKTAVYQYLQDGATMVYNRIDNE
                     PFVDSIAKQIAQFAQAQTVVSGYLAFGSSPSYRNHWDTRDVFAVQLIGKKHWTVSAPN
                     FDMPLYMQQAKDMPHITPPTTVDMEVILEAGDILYIPRGWWHNPMPMNCETFHLAIGT
                     FPPNGYNYMEWLMKKIPDIQSIRQNFIDWEHDQKNIDNAAQAVTEMMKNQENYQAFIQ
                     DFLGNQRVNTAFNMQIFGNLDNDRLPENSTIKLNSLDNRTIKQGYIIANGIKTNLDND
                     SQTILQWIADKHSVKLTQLYEFCQNQNINLEKVEKLVFDLTMIDVLECLTDER"
     gene            2606..2812
                     /locus_tag="WIB82_001465"
     CDS             2606..2812
                     /locus_tag="WIB82_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842751.1"
                     /translation="MFTFLNKNIFIKTIVLGCFYSFLFFSFFVSGKSFGYSFEIFNMD
                     TSALAFLSVFMGFFISVAVHIYDI"
     gene            2836..3171
                     /locus_tag="WIB82_001466"
     CDS             2836..3171
                     /locus_tag="WIB82_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ3842752.1"
                     /translation="MKELTINDLTLVSGGFEFGRDYIEIAPVVGTAGGVASSVRAASM
                     TGEAGAILTRSGVTGLRAGVVAIPLLAYNGTVWVNRNTKLQDNLGRLIDKVTGLDQSG
                     NDYGDGTSY"
     gene            3206..5302
                     /locus_tag="WIB82_001467"
     CDS             3206..5302
                     /locus_tag="WIB82_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ3842753.1"
                     /translation="MSIYLSRLSFGFSRRLLVVLQTEAAECGLACLVSVLGFHGFYTD
                     LRHLRARFSLSLKGATLADLVRFANSMNLTARAVRLDLDELVNLRLPCILHWDLNHFV
                     VLHEVHRNHIVIMNPASGWQKVRMEEVSRCFTGVALELFPNTQFEEKHETRKIRVLPM
                     LRGVVGLKRSLFQLLLLAAVLQVFALASPFFMQWVIDHAIVSADRDLLMTLALCFGLL
                     MIVRQLVSLLQTWAGMYLSTSLNIQWKANVLRRLMDLPVSYFAKRHLGDVVSRFGSVD
                     SIQSTLTSTFFVLVLNSIMAVFTLGLMYLYSPSLTTVVLVVLLIYIGIRWVAYYPLRH
                     ATQENIVHAAKQSSYFMETIRGIQTVKLFDKNAQRHAAWLNLFIDTINTGLMTQKLSA
                     LFGFTNGLLFGIANILIVYFGAVSVLNGGFTVGALMAFMSYKSQFESKAGSLIDQFVQ
                     IKMLGLHAERLADIVLEETENEQNNDFSLPKNLEHFDIQIENVSFSYTENEPYILQNF
                     SLIIKQGTAIAFAGHSGCGKSTLIQILTGSLKPESGHVLLGGHDIHALPPAFIRTWSA
                     SVMQNDVLFTGSIAENISFFDDTPNMEKIAFCAQMANIHHEIVAMPMAYETLIGDMGS
                     ALSGGQKQRIVLARALYREPKILFLDEASSHLDINNERVINDNLRQLNITKIMVAHRQ
                     ETLNTADSVVYLDKVA"
     gene            5304..6566
                     /locus_tag="WIB82_001468"
     CDS             5304..6566
                     /locus_tag="WIB82_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ3842754.1"
                     /translation="MTLFRPEVFQAKKNRWTGQIVLVRPFSLQFLTFFAVSLAAILVA
                     FLIFGSYTNKTTVTGQLLPTTGVVRVYSQDMGVIAHQHVMNGDFVKKGDVLFTLSTSR
                     NDNNGSIQARLLAEAELKKSLSEQEIIMKKRVHAAEKTAQENTVHRFQNQMQHVRNQI
                     IMQEKRIAISEKMLEKQRYLAKMDAISELEKNSYEIALLELKAGLAAYQREADNLARE
                     ITVQQSNLKNLPEQQATEISQLERAVSVYQQEILDYQQRNEQTIRATISGYVSSINTE
                     IGQQVDTNKLLMSIVPKESELLANLYVPSRAIGFVKPNDKVILRYQAYPYQKFGHAEG
                     HVISIAQTALGAQEWTNLGNIFTQTAQVNEPVYLIKVKLDNQHIRIYGTEKKLQIGMI
                     LEADILHENKRLYEWILDPLYQVMGKIS"
     gene            complement(6836..7675)
                     /locus_tag="WIB82_001469"
     CDS             complement(6836..7675)
                     /locus_tag="WIB82_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842755.1"
                     /translation="MGNFAKVSNCEFWDKINYNRAAQAAEQRAKQAQETPKEPPKQEE
                     HFIAKLPNDKLMTIYRLFQKQLDDMRGLERDIERGDWYINARYRMNENRNKIRALEDD
                     NKRLHHDYWAVQDQGENRFFGMGKIGAKLVKENEKQQHINERHGANVREIRRLKTENE
                     KLEGQYSDKLAADVVKVRDRFNEELDNVRREIGKRPMEEFRNVFLQAAQEAQTTIGQK
                     AVQIELEKFPLNGSPSAKRIAFYFGAQRIREVEQKEREDQRKEQERQARRMERDDGHG
                     IGM"
     gene            7716..>7888
                     /locus_tag="WIB82_001470"
     CDS             7716..>7888
                     /locus_tag="WIB82_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ3842756.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPL"
BASE COUNT         2373 a         1433 c         1617 g         2465 t
ORIGIN      
        1 taagtgcgcc cgctgccgcc taaaaggcgg cccggatgcc tgattatggg gcgtccgggg
       61 aggattaagg gggcatttgg gtaaaatcag gagtaattag gagcggaaat agacgaaaac
      121 ctgtgtttgg gtttcggctg ttgggggaag ggcttttttg caaaggtctc aatttaaact
      181 ttaacgcaat atcaactcat tttctgccac atatctcaaa aaagtctagt ttgatacagt
      241 aatctcttta acagcttttc ttgctaaaat ttaaaacaaa atgtaaatat tcttttattt
      301 atttaaataa tagattaaaa tattatttaa ttaaaagcat aatgagcgta gatattcaaa
      361 ggagctaaca tgaaagttat ttccgtatta aatcaaaagg gtggaagtgg taaaaccaca
      421 gtcgcaactc acttggcacg cgccttgcaa ttagcaggaa atagcatctt attggttgat
      481 agcgatccac aaggaagcag tagggactgg gctgctgttc acgaagagca acctgttcct
      541 gtgattggta ttgacagccc gacaattgat cgtgatttaa aaagcgttgt ttaaaaagat
      601 tttgttatta ttgatggcgc accacaaaca gccgatttag ccatatcagc gattaaagcc
      661 tctgatttag ttttaatccc tgttcaaccg tcgccatacg atatttgggc aaccgctgat
      721 ttggttaatt taatcaagca gcggataaaa ctcatagatg gaaatttaaa ggctgctttt
      781 ttaattaacc gaactattaa aggaacaaag attggcggag aaattagagc agcgttagaa
      841 gaatatgagt tacctgtttt atcaaccgtg attgtgcaac gtattattta tccaacatct
      901 gctatcacag gtagcactgt actggataaa gaaccaaatg gagaagcagc taaggaaatt
      961 caatcacttg cgaatgagat aataaatttg ataaatatag ataatcatat ttaatgaaat
     1021 attattttaa atttttaaat attgtaggaa ataattatga acggattaaa agcagatcgt
     1081 cctagtgcaa acaaagcaaa cttatctatt gatgatgtga tcaagcaaga agaaatcaag
     1141 agaatcagtt ttgatatgtc tgaatctctg catagagaat tgaagaaata cgcggctgag
     1201 aatggtgtaa ctattcgcgt cgtgctcaat aaactcgttt cagatttatt gaataaataa
     1261 taacacacgg cagaatgtat ttgaccataa tgtcaaagca atatccaact ttatctaaaa
     1321 ataacaaaat accttcttcc aaaaacagaa cgtcttttca ttgcaaaact tgacatataa
     1381 acactagtta agtaaactta attttcatta ttaataatga aaataaggat tatttttgca
     1441 tattaacttt tcaatggaat ataaagaatt taatgaaaat tatttatata aaaagccatt
     1501 tatttttaaa aatgctttag atgttagttc tatctcatgg aaagaaatta atgagttgta
     1561 tcaacgtgca gaccctactg attggcaatt taaatttcgc aaaggggaaa taatccccaa
     1621 agaagcctat gttgaatcat tcaatgatgt gggtagaata cgtcatcggt ttaataaaac
     1681 agccgtatat cagtatctgc aagatggtgc aacaatggtt tataaccgta ttgataatga
     1741 gccatttgtt gacagtattg ctaaacaaat tgcccagttt gctcaagcac aaactgttgt
     1801 tagtggttat ttggcgtttg gtagttctcc atcatatcgt aatcattggg atactcgtga
     1861 tgtgtttgcg gttcaattga ttggcaaaaa acactggaca gtttctgctc caaattttga
     1921 tatgccattg tatatgcagc aagcaaaaga tatgccgcac attactccgc ctacaacagt
     1981 ggatatggaa gtcattttag aagcaggaga tattttatat atcccgcgtg gttggtggca
     2041 taatcctatg cctatgaatt gtgaaacatt ccatttagct attggaactt ttccgccaaa
     2101 tggttataat tatatggaat ggttgatgaa aaaaatacct gatatccaga gtattcgtca
     2161 aaatttcata gactgggaac acgaccaaaa aaatatagat aatgcggctc aggcagttac
     2221 tgaaatgatg aagaaccaag aaaattatca agcattcata caagattttt tgggaaatca
     2281 acgcgtaaat actgcattta atatgcaaat ttttggaaat ctggataatg acagattacc
     2341 tgaaaatagt acaattaaac tcaattcgtt ggataaccgt acaattaaac aaggatatat
     2401 catagcgaat ggcatcaaaa ccaacttgga caatgatagc caaacgattt tgcaatggat
     2461 tgctgacaaa catagtgtta agttaactca actgtatgaa ttttgccaaa atcaaaacat
     2521 taatttggaa aaagtggaaa aattagtgtt tgatttgaca atgatcgatg tattggaatg
     2581 tttaactgat gaaagataaa ttattatgtt tactttttta aataaaaata tatttatcaa
     2641 aacaattgta cttggctgtt tttattcctt tttatttttt agtttttttg tatctggtaa
     2701 gtcatttggt tattcatttg aaatttttaa tatggataca agtgcgcttg cttttttaag
     2761 tgtatttatg ggctttttta taagcgtggc tgtgcatatt tacgatattt aatacaattg
     2821 aaaagataaa taattatgaa agaattaact attaatgatt taaccctagt ttcgggtgga
     2881 tttgaatttg gtcgtgatta tattgaaata gctccagttg taggtacagc aggtggcgtt
     2941 gcctcttctg tgagagcagc ttcaatgact ggagaagctg gagcaattct tactcgttca
     3001 ggagtaacag gattacgtgc tggagtggta gctattccat tgttagccta caatggtact
     3061 gtatgggtta atagaaatac caaattacaa gataatttag gtagattaat agataaagta
     3121 actggcctgg atcaatcagg caatgattat ggcgatggga caagttatta atctcataat
     3181 actttaaaat caaaaaagaa caccaatgtc aatatatcta tctcgcctat cattcggctt
     3241 ttctcgccgt ttactagttg tcttgcaaac ggaagcagca gaatgtggat tggcgtgttt
     3301 ggtatcggta ttggggtttc atggttttta tacggatttg cgccatttaa gggcgcgttt
     3361 ttctttgtct cttaaaggcg caacattggc agatttggta cggtttgcta atagcatgaa
     3421 tttaactgct cgagctgttc ggttggattt agatgaattg gtaaatttgc gcttaccctg
     3481 tattttacat tgggatttaa atcattttgt ggtgttgcat gaagttcatc gtaatcacat
     3541 tgtgattatg aaccctgctt cgggttggca aaaagtgcgt atggaagaag tctcgcgctg
     3601 ttttacaggt gtagcgttgg aactatttcc taacacgcaa tttgaagaga aacacgaaac
     3661 gcgtaaaatt cgcgtactac cgatgttgcg cggagtggtg ggattaaaaa ggtctttatt
     3721 tcagcttctg ctgctggcgg cagtattaca ggtgtttgct ttggcaagcc ctttttttat
     3781 gcaatgggta atagaccatg ccattgtatc ggcagaccgt gatttattga tgacattggc
     3841 attgtgtttt ggcttgttga tgattgtgcg gcagttagtg tcattgttgc aaacttgggc
     3901 gggtatgtac ttatctacct ctttgaatat tcaatggaaa gctaatgtat tacgccgctt
     3961 aatggatttg cctgtttctt atttcgctaa acgacatttg ggcgatgtgg tttcacgttt
     4021 tggctcagtg gacagtattc aaagtacgct cacttctact ttctttgtgt tggtgctcaa
     4081 cagcattatg gcggtattta cattagggtt gatgtatttg tatagcccaa gtttaacgac
     4141 agttgtactg gttgttttgc tgatttatat tggtattcgt tgggtggcat attatccatt
     4201 acgccatgca actcaagaaa atattgttca tgctgccaag cagagcagtt atttcatgga
     4261 aaccatacgt ggtattcaaa ctgttaaatt atttgataaa aatgcacaac gccatgcggc
     4321 ttggctgaac ttgtttattg acacgattaa cacaggttta atgacacaaa aactttccgc
     4381 gctgttcggt tttacgaatg gccttttgtt tggtatcgct aatattttaa ttgtgtattt
     4441 tggtgcggtt tctgtattga acggtggttt taccgttggg gcgttaatgg cgtttatgtc
     4501 ctacaaaagt cagtttgaga gtaaagcagg ttcattgatt gaccagtttg tacaaatcaa
     4561 aatgctagga ttacacgcgg aacgcttggc ggatattgtg ctagaagaaa cggaaaatga
     4621 acaaaacaac gatttcagtc tgcctaaaaa tttagaacat tttgatattc aaatagaaaa
     4681 tgtatcattt agctatactg aaaatgaacc atacattttg caaaatttca gtttaatcat
     4741 caaacaagga acagccattg cgtttgctgg acattcaggc tgtgggaaat ctacgctcat
     4801 tcaaattctg acaggcagtc tgaaacctga aagcggtcat gtgttattag gtgggcatga
     4861 cattcatgcg ttaccacctg catttatccg cacatggagc gcaagtgtga tgcaaaatga
     4921 tgtgttattt actggctcaa tcgcagaaaa catcagtttt tttgatgata cgcccaatat
     4981 ggaaaaaatc gcattttgcg ctcaaatggc gaatattcat catgaaattg tcgctatgcc
     5041 tatggcgtat gaaacattga ttggcgacat ggggagtgca ttgagtggtg ggcaaaagca
     5101 gcgcatcgta ctagctcgtg ctttatatcg tgaaccaaaa attttgtttt tagatgaagc
     5161 cagtagccat ttggacatca acaatgaacg tgtgattaac gataatctac gccaactcaa
     5221 cattaccaaa atcatggtcg cgcatcgtca ggaaacttta aatactgctg atagcgtagt
     5281 ttatttagac aaggtagcct gaaatgacat tatttcgccc cgaagtattc caagctaaaa
     5341 aaaatcgctg gacagggcaa attgttttag tgcgtccttt ttccttgcag tttttaacat
     5401 ttttcgcggt ctcattagcg gctattttag ttgctttttt aatttttggg agctatacca
     5461 ataaaaccac cgtaacagga caacttttgc cgacaacagg ggtagtacgc gtatattcgc
     5521 aggatatggg ggttattgct catcaacacg ttatgaatgg cgattttgtg aaaaaaggcg
     5581 atgtgttatt tacattatct acttcacgaa atgacaataa tggcagcata caagcacgat
     5641 tactcgccga agctgaatta aaaaaatcac tatcagaaca agaaattatt atgaaaaaac
     5701 gtgttcatgc agcagaaaaa accgcacaag aaaacacggt acaccgtttt cagaaccaaa
     5761 tgcaacacgt tagaaatcaa attatcatgc aagaaaaacg tattgcgatt tctgaaaaaa
     5821 tgttagaaaa acagcgttat ttagccaaaa tggacgcaat ttctgaatta gaaaaaaata
     5881 gttatgaaat tgctttattg gagctgaaag caggtttagc cgcttaccaa cgagaagcag
     5941 ataatcttgc tcgggaaata actgtacagc aaagcaatct aaaaaatctg cctgaacagc
     6001 aagctactga aatcagtcaa ttagaacgcg cagtatctgt ctatcaacaa gaaatattgg
     6061 attatcaaca acgtaacgaa caaactattc gcgcaaccat atcaggctat gtcagttcta
     6121 ttaacacgga aattggtcag caggtagata ctaacaaact attgatgagt atcgtgccga
     6181 aagaaagtga attactggct aatttatatg tccctagtcg cgcaatcggt tttgtaaaac
     6241 caaatgacaa ggtaattttg cgttatcaag catatcctta tcaaaaattc ggacacgcag
     6301 aaggacacgt catttctatt gctcaaacgg cactaggggc gcaagaatgg acaaatttag
     6361 gcaatatttt tactcaaaca gcacaagtga atgagcctgt ttatttaatc aaagtaaaac
     6421 tggataatca gcatattcgt atatatggca cggagaagaa attgcaaatt ggtatgatac
     6481 tagaagccga tattttgcat gagaataaac ggttatatga atggatactt gaccctttgt
     6541 atcaagtaat gggaaaaata tcatagctga attgctcaat acattggagc gtatgagtgc
     6601 ccctaattat caagcaaaac caatgtccga tgacttccaa tttgttgtta agcacaaata
     6661 cacagtgttt tatgtaataa tgaagacaga gaagtgatac aatttttcgc tccatttttg
     6721 cttcctgaat tgctgcaatt gtatctgcgt tgtatgcgag aagtaaatat tttcgatgca
     6781 tttgctgact ttgtaccata atacgtcttc caaccagtat gataggctgg tttttctaca
     6841 tgccgatgcc gtggccgtcg tcccgctcca tacgccgcgc ctgccgctcc tgctctttgc
     6901 gctggtcttc ccgctccttc tgctctactt cccttatccg ctgcgcgccg aaataaaacg
     6961 cgattcgctt ggccgaaggg ctgccgttca gggggaattt ttccagttcg atttgaaccg
     7021 ccttctgccc tattgtcgtc tgtgcttctt gggctgcctg caaaaatacg ttcctgaatt
     7081 cttccatcgg ccgtttgccg atttccctgc ggacattgtc cagttcttcg ttaaacctgt
     7141 cgcgtacttt gaccacgtcg gcggccaact tgtcgctata ctgcccttcc agtttttcgt
     7201 tttctgtttt cagccgccgg atttctcgca cgttcgctcc atgccgttcg ttgatgtgtt
     7261 gctgcttctc gttttcctta accagttttg cgccgatttt acccatgccg aaaaagcggt
     7321 tttcgccttg gtcttggact gcccaatagt cgtgatgcag ccgtttgttg tcgtcttcca
     7381 gcgcccttat tttgtttcgg ttttcgttca tgcggtatcg cgcattgatg taccaatctc
     7441 cgcgttctat atcgcgctcc aagccgcgca tgtcgtccag ttgtttttga aacagcctgt
     7501 atatcgtcat tagcttgtcg ttcggcagct tggcgataaa gtgctcttcc tgtttcggcg
     7561 gctctttcgg tgtttcctgc gcctgctttg cccgctgctc ggcggcttgg gcagcccgat
     7621 tataattaat tttatcccag aattcacaat ttgagacctt tgcaaaattc cccaaaatcc
     7681 cctaaattcc caccaagaca tttaggggat gttccatgag caccttcttc caacaaaccg
     7741 cccaagccat gattgccaaa cacatcgacc gtttcccgct attgaagttg gaccaggtga
     7801 ttgattggca gccgatcgaa cagtacctga accgtcaaaa aacccgttac cttagagatc
     7861 accgcggccg tcccgcctac ccgctgtt
//
LOCUS       DAWHRS010000095         7863 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWHRS010000095 DAWHRS010000000
VERSION     DAWHRS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.095"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1337)
                     /locus_tag="WIB82_001471"
     CDS             complement(<1..1337)
                     /locus_tag="WIB82_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ3842757.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLETHTDTRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTYRPETSRTERAGYVEKLQIRLSG"
     gene            complement(1399..2748)
                     /locus_tag="WIB82_001472"
     CDS             complement(1399..2748)
                     /locus_tag="WIB82_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ3842758.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVVAATFAFFAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAMLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVVEGRLNGNIADARAWSGLLVGSIACYGILPRFLAWAVCKILLKTSK
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKTNDRT"
     gene            2891..3517
                     /locus_tag="WIB82_001473"
     CDS             2891..3517
                     /locus_tag="WIB82_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ3842759.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3581..4999)
                     /locus_tag="WIB82_001474"
     CDS             complement(3581..4999)
                     /locus_tag="WIB82_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ3842760.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            5326..6054
                     /locus_tag="WIB82_001475"
     CDS             5326..6054
                     /locus_tag="WIB82_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ3842761.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6113..6601)
                     /locus_tag="WIB82_001476"
     CDS             complement(6113..6601)
                     /locus_tag="WIB82_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ3842762.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRGRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVALTVWWAWAR
                     AK"
     gene            complement(7359..7823)
                     /locus_tag="WIB82_001477"
     CDS             complement(7359..7823)
                     /locus_tag="WIB82_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ3842763.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2034 a         2120 c         1974 g         1735 t
ORIGIN      
        1 tttcccgaca ggcggatttg aagtttctcc acatatccgg cgcgttcggt acggctggtt
       61 tcggggcggt acgtattgag cgacgaccat ttccaatgac tgcgggcttt gttgagttcg
      121 ggcgggagtc tggcggcgtc ccacggggct ttgcggctgt gcagctcgat atccgactgt
      181 gccgcgtgtc cgcgcgtttg caggacgtgg agcaaatcga gggcgcgggc ggcgagcagg
      241 gtcaaggttt cagggtcggt gtgcaaggtt tggcggccgg agagtttgtc ggaaatggtg
      301 cgggtattgg gcaggatgcc gcccaaaaag ccgccgattg ccgtacccaa tccgagcgag
      361 ccgccgagtg tggcgatgtc cagccccaag ccgatgagcg cgccggttgc cgcgcccgtg
      421 ccggtgcgga tgccgtattg tttgagcaat tcgctgtcga acgggtcttg gcggaaggct
      481 tgcggcatcc agtcgccgcc gtcgatttcg ctgtggtaga aacggtagag ggcaaacagc
      541 cgctgctgca tctgccgttc gagttggcgt atttccgcct gcatggtttg cagcacggtg
      601 gcggtatcct cgttttcgtc cacttcctgc ctgaaggcgg cggcatcgag caggaaatcg
      661 gcgatttcgc ggcgcgcttc gccgtccagc cgctgccatt cgcgccggcg catggctgtc
      721 aggcggtcaa gtgtgctgcg ttcgggcaac atggtggcga ggttttccca caggcgtagt
      781 tcgccttcaa aatcaaaggc gacggtgtcg aaccctgcga aaacgtgcag gtttctcctc
      841 gccagcatgg ttgtccacga ttcgggaagc tgtccgccgg taaagttgaa cacgggcata
      901 accggtttgg cacaccatga aaggatggtc aattcatccc gatatttgtc gaggacgggt
      961 tcgcgcgcgt cgatgacgta cattgccata tcgctttgca agacttgccg taagactttg
     1021 gcttcctgat tgaaatcatg gtgcgcaccg tggctgccga gaaactgttg cagccgctcg
     1081 atgccgtctg aacgcgtatc cgtatgggtt tccagccatt ccaaaacacc gcccgcgtct
     1141 tcgagtccgg gcgtgtcata caggaaaacc agcatgtccg cgccgtcgct gatggcggct
     1201 tcttcgacat ggcgcgtagt ggacggggca tttttgactt cgccgaagcc gctgtcgcgc
     1261 aatagggtgc gcaggagcga ggttttgccg gtattggtat gcccgacgac ggcgagggaa
     1321 aggggttgtt tgttcatgat gtttttgaag aatggatttt cagacgacct tttaaggatg
     1381 gcggctttat ggggcgtttc aagtgcggtc gttggttttc agacggcctt cctgcgccgc
     1441 cctgtcaggc tcaagccacg ccgcgccgca ttcggtcagc gcgttacgcc aatgttccag
     1501 cttttccgaa aggtcgtctg aaagcccctg ttccgccaaa agctgcacca ccgcgccgcc
     1561 ctgcgccgct tccgaaagtc ggacgatctg ccgcaacacg ccgcggtcgg gcacagtttg
     1621 ggcgcgcacg ccgataagca gttgcgccgg tttctgcttc agctctgtct ccagcgcggc
     1681 aacctgttcc cgattggcgg caacgccctt atccagccat tcctgcgcca gcctgccctc
     1741 gaaccattcg ccgtcctgcc attcggtctc cagcatgacc gcccatttcg gcgcatcgtt
     1801 caagacgatt ttcggcgaaa cggcggacac ggtttcccga cgcgtatccg catcggtgat
     1861 tttgttctgc cagcggcgga tgaccgcctg ataatagggt ttttccaaat ccaagccgtt
     1921 tttgcttgtt ttcaaaagga ttttgcatac cgcccaagcc aagaagcgcg gcaggatgcc
     1981 gtagcaggcg atactgccga ccagcagccc cgaccaagcc cgcgcatcgg caatattgcc
     2041 gttcagacga ccttcaacta ccgcccgcgc atcggggaca gggaaaccga gtttcgacgg
     2101 cagccatgcc aacatttcca ccgcgcgtac cgaagcggca ttgctcaaca gcgtgctttc
     2161 ccagttgaac gtatattggc gcaccaaaag cagcaacaat accgacacca gcattccgag
     2221 cagcgtgcag agccacaggc tgtgcgacgt tgcgcctatt ttccaacgta ccgaaggttg
     2281 ccgccactcg tccgcataca gccgcaacat cgcctgattg acagggtctt tgccccgaaa
     2341 ccacgtcgcc ggactgctga aaaaacgccc cactttcacg cgcaggaaca acattgccaa
     2401 ccatactgcc agcatcagcg tattcatgcc caacacgccc gccaaaacca aaaagaaatt
     2461 cagcccctga ttgtccatta gaagataagt gcctgaaaaa ccggcaaaaa acgcaaacgt
     2521 cgccgccacc acccacaacc agaacgaccc cgcacgcaca cgttccaacg tctcccgcag
     2581 catacggttc ctgtcaatca tctccgcccg acggatgatt ttttcctccg tactgccgtc
     2641 cacgcggcgc aaagcctccg tcgcctgcac gggatcgccg ctgaaaataa agccgccttc
     2701 ttccaaaata cggaccagct caaccagttt tcgggatgga ttcaacataa aatgccgtct
     2761 gaaaataaaa aacagatttt aacacacgca ttttcaagaa tattcacagt gtaggcaaag
     2821 agtaaatccc acacagaggc aaaagtatcg gcgtaaactg actgcctcta ctttcccgaa
     2881 agattgtgcg atgtatacag gcgaacgctt caatacttac agccatttga gcggtttgat
     2941 tctggcggcg gcaggtttgg tgctgatgct gctgaaaacc ataggacacg gggacggcta
     3001 ccgtatcttc agcgtatcgg tttacggcat cagccttctt ctgctctatt tgagttcctc
     3061 gctgtaccac ggaattgcag ccggaaaact gaaaagcatt ttgaaaaaaa ccgaccactg
     3121 catgatttat gtcctgattg ccggaagcta cacaccgttt gcactggttt ctttgagaaa
     3181 cgggccgggc tggacggtat tttcactgtc ctggctgctg gcggctgcag gaatcgcaca
     3241 agaactcacc atcggacgga aaagcgaaaa acgtctgctg tctattgtga tttatgtcgt
     3301 catgggttgg atggtcttgg cggtaatgaa atccctgaca gcctcactcc cgtcggcagg
     3361 actggcttgg ctggcggcag gcggtatgct gtacagcgtc ggcatttact ggtttgtaaa
     3421 cgatgaaaaa atccgacacg ggcacggaat ctggcatctg ttcgtattgg gcggcagcat
     3481 cacccaattt gtcagcgtgt acggttacgt aatctgaatg ccgtctgaaa agcaaaacct
     3541 cccgatcctg aagattggga ggttttctgt ttgccggaca tcagcccttg tcgtggaact
     3601 cgtgaaattc atactgatag gacaaatccc gacccgcttt tttctgtgcc aaataatcat
     3661 cataaatggc acggatttcc ttacgcaaca aaaacagggc tatcaagttc gggataacca
     3721 taaagccgtt gaacatatcg gacaggctcc aaaccaaatc gactttgccg agcgtaccca
     3781 aaacgatggc aagcagaacc aatgcgcgat agatgcccaa gtgtcttccc ctgaaaagaa
     3841 aacggatgtt ggactcaccg aaataatacc agccgataat ggtcgtcaag gcgaaaaagg
     3901 tcagacacac ggcaagcaat tgcgaaccga agcccggaaa tgccttgtta aaggcaaatt
     3961 gagtaaccgc cgcgccctgt tcgcccgaaa ggttggcatc agtcagcagg ataatcaatg
     4021 ccgtagccgt acataccaaa atcgtatcga taaacacacc gacaaatgcc gccatacctt
     4081 gctgcacagg atgcttcaca tcagcggttg catgtgcatg cggggtagaa cccatacctg
     4141 cttcgttgga aaacagaccg cgcgccacgc cgaaacgtat cgcttcacgc ataccgatac
     4201 ccgcagcacc gcccaaaacg gcttcaggat tgaaggcggc ggtaaagatg tggttgaaca
     4261 tcggcacaat atggtcggaa aattcaaaca ggataacgac ggcgcacaaa atataaacaa
     4321 ccgccataaa cggcacgaca aactgggcaa tattggcaat acggttcacg ccgccaatca
     4381 caaccatgcc cgcaaggacg gcaagcacaa taccgactgc caaagaaggc acatcaaatg
     4441 caatggtaac ggcagaagca atggagtttg cctgtgtcgc attaccgata aagcccaatg
     4501 cgataatcaa cgcaatggag aaaaagcccg acaggaaacg cgccgcgccc ctgccgattt
     4561 tcggagtcag accgtgcgta atataaaacg ccggcccacc gatgtatttg ccgtggctga
     4621 cgacgcggta tttctgcgcc agcagtgcct ccgcaaaaat cgtggacatc cccaaaacgg
     4681 cagaaaccca catccaaaaa atcgcgcccg gcccgcctgc ggtgatggcg gtcgccacgc
     4741 cggcgacgtt gcccgtaccg atttgcgcag atatggcaac cgccaacgcc tgaaactgcg
     4801 ataaagactt gtcgtcttta tcgcctttgg caaacaagcc gccgaatacg gatttgaatc
     4861 ccgcgcccag cttggtaatc tgcggcgcac cgagatacag cgtaaaaaac aggccgatac
     4921 ccaaaagcgc gtaaatcagc aggtagtccc aaaggaaccg attgactgta cccaccagaa
     4981 cagacaatat attttccata aaataaacct tatcttacaa ttaaaatgac tgccttccaa
     5041 aagacattcc aataaggaaa cacggcgagc agaccgtatt tgccgcaaca gatgccttaa
     5101 attgtcaaca atcggggaga agctgcgcca tcataccgta aaatatcgtt aattaaaaca
     5161 tttctttatt tttaagcgga aagcggagga aatcgctttc agacggcatc gacaacggca
     5221 cggcataaaa caggatattt tgggtacttg caacttatgt taaaatgccg accgtaaaaa
     5281 atctgacaaa aacagattaa ttatttgaaa taagaaagga aatttatggc aggccatagc
     5341 aagtgggcga atatccagca taaaaaagcc cgtcaggatg ccaaacgcgg caaaatcttc
     5401 acccgtttaa tcaaagaaat caccgttgcg gcgcgtatgg gcggcggcga tcccggcgca
     5461 aatccgcgcc tgcgcctggc tttggaaaaa gcagccgaaa acaatatgcc caaagacaat
     5521 gtgcaacgcg ccatcgacaa aggtacgggc aacttggaag gcgtggaata catcgaattg
     5581 cgctacgaag gctacggcat cggcggcgcg gctttgatgg tggactgcct gaccgacaac
     5641 aaaacccgca ccgttgcgga cgtacgccac gcgtttacca aaaacggcgg caacttgggt
     5701 accgacggct gcgtggcgtt caacttcgtg catcagggct atttggtatt cgaacccggc
     5761 gttgacgaag acgcgctgat ggaagcggct ttggaagccg gtgcggaaga cgtggttacc
     5821 aacgacgacg gttccatcga agtcattacc gcgccaaacg attgggcggg cgtaaaatcc
     5881 gctttggagg cggcaggtta caaatccgtt gacggcgacg ttacgatgcg cgcccaaaac
     5941 gaaaccgaac tctccggcga cgatgccgtc aaaatgcaaa aactgattga cgcactggaa
     6001 gatttggacg acgtgcaaga cgtttacact tccgccgtat tgaatttgga ctgatacgca
     6061 gcacagcaga catacaataa aatgccgtct gaacctttca gacggcattg atttatttag
     6121 cccttgccca cgcccaccaa accgtcaggg caaccgccag aaaatacgcc acactccaaa
     6181 caggcaaagc aacccccagc agataatccg gttcggcaca atttccgaac ccgcgcacga
     6241 caggctcgaa ccaatcaaac aaaggccagc ccttcaatcg aaacgtccac ggcgcaccgc
     6301 acgaaggagc cgtacccggc ggcagcgact gcagccacaa ctgatatgcc gcaacagaaa
     6361 tacccgtaac ggcaggaatg ctgataaaga cagcaccgaa caaaccgcct gcccttcctc
     6421 ttggtctgca catcaggaca attgccgtac acaatgcggt tgccaaaacg cataaccgct
     6481 gactgataca caaaacgcaa ggctccatac ccaaaacata ctgtgccgcc aaagaaccgg
     6541 caaatgcaca gaccgaaacg gcaaacagca gccaaacggc ttttctaaat aacggggtca
     6601 ttttctcaac acaccaatca aaataccgat atgccgattt tgctggatat atcccgagat
     6661 ggcaagggac gaatcgacac cggcgcaggt gcgggcgatt tcggttttcc cgatatgcac
     6721 aaaacctgac aagggacaaa acggcgattt gcccgcacaa tgcccacaaa aaaatacatc
     6781 cggaggattt gaatttcagc aaatttagcg aatcaaaagt ttatttcaat gaaatcatat
     6841 gatttttttg aataagcgga ttgatgggtt tttgaaggaa tttgttaccg gatagccatc
     6901 gggcaagttt tttgcaaaat ttgaatcggt cgggtaaatt ttcaaaaaat aattgacagc
     6961 ggataagaaa cggcggataa ttcccgccgt cgagttgctt gatgcagctt gatttttctc
     7021 ctctatttct cctttgtaga cttggcacac attcaactgg atgtgtgcat ttttttatct
     7081 ccgctttttt tgaaatttaa ttacttttat tgtttggaat tccattcttt aagaaattta
     7141 acaagaaaaa cgcttgcttt ttgaccggaa aagctgcata attcattccg ttagctggtt
     7201 cgagtagtca gttaatagtt tctcctctat ttctcctttg tagacttggc acacattcta
     7261 ttgaatgtgt gcattttttt atctgaagca acaagcctct gtgcgtgatg ttgttatgtt
     7321 tcatttaggt gtcaaaccgc atatccggtc tgaaatattc aatccaaatc caaaaccgga
     7381 ttttctttga cctcctccat cacaacataa ctcctactct ccgaagcggc aggcagttgc
     7441 aataagatat tgcctagcat atcccgatag gcagacatat cgggcaaacg tactttaatc
     7501 agatagtcgt attcgcccga caccaagtgg cattccataa tttgcggaat tttcagcact
     7561 tcttttttga aatcttcgaa aatattgccc gatttggatt gcagcttcag ctcgacaaaa
     7621 accaatagcg gtttgcccaa cagatgggga ttgaggtggg cgtgatagcc ggaaatataa
     7681 tgttcccgct ccaaacggcg caccctctct gtaacgggcg tggtggacaa gcctaccttc
     7741 tcggcaagct ccgtcatcgg gatgcgggca ttctgttgga ggatcttaag aatgcggaaa
     7801 tcgattttat ctagttcttt catttggatt gccttgtatt tattattgat tttaacaaat
     7861 aga
//
LOCUS       DAWHRS010000096         7858 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWHRS010000096 DAWHRS010000000
VERSION     DAWHRS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.096"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            51..1220
                     /gene="prpF"
                     /locus_tag="WIB82_001478"
     CDS             51..1220
                     /gene="prpF"
                     /locus_tag="WIB82_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ3842764.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1634..2833
                     /locus_tag="WIB82_001479"
     CDS             1634..2833
                     /locus_tag="WIB82_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ3842765.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            2941..3561
                     /locus_tag="WIB82_001480"
     CDS             2941..3561
                     /locus_tag="WIB82_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ3842766.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(3688..4131)
                     /locus_tag="WIB82_001481"
     CDS             complement(3688..4131)
                     /locus_tag="WIB82_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ3842767.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4305..4673
                     /locus_tag="WIB82_001482"
     CDS             4305..4673
                     /locus_tag="WIB82_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ3842768.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4718..5872
                     /locus_tag="WIB82_001483"
     CDS             4718..5872
                     /locus_tag="WIB82_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ3842769.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(5929..6801)
                     /locus_tag="WIB82_001484"
     CDS             complement(5929..6801)
                     /locus_tag="WIB82_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ3842770.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            6886..7698
                     /locus_tag="WIB82_001485"
     CDS             6886..7698
                     /locus_tag="WIB82_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ3842771.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSSEAGKVMNTLL
                     VYGRDGVRTGLYHKMHLFGFSGLGERYAEADTISAGRAVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRTRAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1692 a         2164 c         2287 g         1715 t
ORIGIN      
        1 cactataaaa atcgtgggca gagcccacgc tacataagga gaatctagaa atgccgcaaa
       61 ttaaaattcc cgccgtttac taccgtggcg gtacatcaaa aggcgtgttt ttcaaacgtt
      121 ccgacctgcc cgaggcggcg cgggaagcgg gaagcgcacg cgacaaaatc ctcttgcgcg
      181 tactcggcag cccggacccc tacggcaagc agatagacgg tttgggcaac gccagctcgt
      241 ccaccagcaa ggcggtgatt ttggacaagt ccgaacgcgc cgatcacgat gtcgattacc
      301 ttttcgggca agtttccatc gacaaacctt ttgtcgattg gagtggcaac tgcggcaacc
      361 tcaccgccgc cgtgggcgca tttgccatcg agcaaggctt ggtcgataaa ggcaagattc
      421 cttcagacgg catctgcacg gtcaaaatct ggcagaaaaa catcggcaaa accattattg
      481 cccatgtacc gatgcaaaac ggcgcagttt tggaaacagg cgattttgag ctcgacggcg
      541 taacgttccc ggcagccgaa gtacaaatcg aatttcttga tccagccgac ggtgaaggca
      601 gtatgttccc aaccggcaat ttggtcgatg aaattgatgt gccgaatata ggccgtttga
      661 aagccacgct catcaacgcg ggcattccga ccgttttcct gaatgccgcc gacttgggct
      721 acacgggcaa agagttgcaa gacgacatca acaacgatgc cgcggctttg gaaaaattcg
      781 agaaaatccg cgcttacggt gcgctgaaaa tgggtctgat cagcgacgta tccgaagctg
      841 ccgctcgcgc gcacacgccg aaagtcgcct tcgtcgcgcc cgccgccgat tacaccgcct
      901 ccagtggcaa aaccgtgaac gccgccgaca tcgatttgct ggtacgcgcc ctgagcatgg
      961 gcaaattgca ccacgcgatg atgggtaccg cctctgttgc cattgcgacc gccgccgccg
     1021 tgcccggtac gctggtcaac cttgccgcag gcggcggaac gcgtaaagaa gtgcgcttcg
     1081 ggcatccttc cggcacattg cgcgtcggtg cagccgccga atgtcaggac ggacaatgga
     1141 cggccaccaa agcggttatg agccgcagcg cacgcgtgat gatggaaggt tgggtcaggg
     1201 tgccggaaga ttgtttttaa attgacgtag catgggtttg cccgcgagcc ataaacaggt
     1261 cgtctgaaaa acaagtaaac atcaaatcac tgaccattcc tttcccttgc cctgtggcgg
     1321 aaggcggcaa atcacaagga agaacacgga aaccccgata aaagacagct tcccgtatta
     1381 ccgtcattcc cgcgcaggcg ggaatctaga cctgtcaata tggaggattg gtaggggaaa
     1441 acaggtttcg tgagttctac attctggatt cccgccacag cctgtcctcg cgtaggcggg
     1501 gacggaataa cgatagaaaa tgcggcatac gctttgccca aagaggccgt ctgaaacacc
     1561 ttgcgcctga tgtctgcctt tttcagacga ccccacacca aaaaaacaac cacaaactac
     1621 aaggagaaac atcatgtccg accaactcat cctcgttctg aactgcggca gttcatcgct
     1681 caaaggcgcc gttatcgacc gaaaaagcgg cagcgtcgtc ctaagctgcc tcggcgaacg
     1741 cctgaccacg cccgaagccg tcattacgtt caacaaagac ggcaacaaac gccaagttcc
     1801 cctgagcggc cgaaattgcc acgccggcgc ggtgggtatg cttttgaacg aactggaaaa
     1861 acacggtctg cacgaccgca tcaaagccat cggccaccgc atcgcccacg gcggcgaaaa
     1921 atacagcgag tctgttttga tcgaccaggc cgtaatggac gaactcaatg cctgcattcc
     1981 gcttgcgccg ctgcacaacc ccgccaacat cagcggcatc cttgccgcac aggaacattt
     2041 ccccggtctg cccaatgtcg gcgtgatgga tacttcgttc caccaaacca tgccggagcg
     2101 tgcctacact tatgccgtgc cgcgcgagtt gcgtaaaaaa tacgctttcc gccgctacgg
     2161 tttccacggc accagtatgc gttacgttgc ccctgaagcc gcacgcatct tgggcaaacc
     2221 tctggaagac atccgcatga ttattgccca cttaggcaac ggcgcatcca ttaccgccat
     2281 caaaaacggc aaatccgtcg ataccagtat gggtttcacg ccgatcgaag gtttggtaat
     2341 gggtacacgt tgcggcgaca tcgatccggg cgtatacagc tatctgactt cccacgccgg
     2401 gatggatgtt gcccaagtgg atgaaatgct gaacaaaaaa tcaggtttgc tcggtatttc
     2461 cgaactttcc aacgactgcc gcaccctcga aatcgccgcc gacgaaggcc acgaaggcgc
     2521 gcgcctcgcc ctcgaagtca tgacctaccg cctcgccaaa tacatcgctt cgatggctgt
     2581 ggcctgcggc ggcgttgacg cactcgtgtt caccggcggt atcggcgaaa actcgcgtaa
     2641 tatccgtgcc aaaaccgttt cctatcttga tttcttgggt ctgcacatcg acaccaaagc
     2701 caatatggaa aaacgctacg gcaattcggg cattatcagc ccgaccgatt cttctccggc
     2761 tgttttggtt gtcccgacca atgaagaact gatgattgcc tgcgacactg ccgaacttgc
     2821 cggcatcttg tagccaaaaa agggacgagt ccgcaaaaat gccgtctgaa attttcagac
     2881 ggcattttgt ttggattttg ggcgtacaat acggaaaaca tgacgataag gacacaaacc
     2941 atggcacagt ttttcgctat tcatcccgac aatccccaag aacgcctcat caagcaggcg
     3001 gttgaaatcg tcaatcaagg cggcgtggtg gtttatccga ccgattcgtg ttacgccttg
     3061 ggttgcaaac tcggcgataa ggcggcgatg gaacgcatac tctccatccg caaaatcgat
     3121 ttgaaacacc acctgaccct gatgtgcgcc gatttgagcg agttgggcac gtacgccaaa
     3181 attgacaacg tacagtttcg tcagcttaaa gccgccacgc ccgggcctta tacctttatc
     3241 ttgcaggcga cgaaggaagt gccggcgcgc acgctgcacc cgaaacgcaa aaccatcggg
     3301 ctgcgtattc ccgataatgc cattgcacaa gccctgctgg gggaattggg cgagccgctt
     3361 ttaagctgca ccctgatgct gcccgaagac ggcgaaccat tgaccgatcc ttatgaaatc
     3421 cgcgagcgtt tggaacacgc cgtcgatttg gtgattgacg gcggctggtg cggaaccgag
     3481 ccgaccaccg tcgtcgatat gaccgacggc acggaactgg tgcgccgggg ttgtggcgat
     3541 acggcggtgt tcggtttgta gggaaaccga tgccgtctga agcatcggct gttcagacgg
     3601 cattgcgcgc cttgccggcg gcagtccgaa atgccggcgc gtatcgcgct cggtcggaat
     3661 atccgtttga aacggcattt tgatgcatta ctgcaccgca atcggaattc tcggttcgta
     3721 gagcaggtcg taggtcggct tgttgagcag gtcttggagc gtgaaaccgt ccagatacgt
     3781 gaaaaacgac ttcattgcac cgccgagtat gcctgtcaga cggcaggacg gtgtaatcag
     3841 gcattcgttg ttcgaaccca tacattcgac cagctgcatc ggttcgaggt ggcggacgac
     3901 cgagccgatg ttgatgcggt cgggcggtgt ggcaagccgc agaccgccgc cttttccgcg
     3961 cacactgtgg aggaagccgc ctttgaccag cgcggtaacg accttcatca gatggctttt
     4021 ggaaatgccg taggttacgg cgatggtact gatgttgacc agcgcatcgt cgttgatggc
     4081 agtgtagata aggacgcgca gcccgtagtc cgtatgttgt gtcaaataca tgattttctc
     4141 ggtatggatt gttattctta tcggtacggt ttaaggttca cggacaatac cttaatggtt
     4201 gaaaccctgt ccgtcggggc ggtagaatgc agcctgtctg cggcggtatg ccgtctgaaa
     4261 catccgcgct accgtttgag aatttgttat tgtaactcaa aatcatgaaa ccgttgaaac
     4321 gacatcccgc ccttatcggg ctttcgcgtg accaccacca ttcgctttcc ttgtgcgtgc
     4381 gtctgttgcg gacgccggaa gaaaggcatc gggacgaact cgaaccgcat ttttccgaat
     4441 tggaaaccca ttttcgcgaa gaagaaacca agtttgcccc aatttggcag aatgtcgccc
     4501 ccgaattgaa acaacgtttc gagaaagacc acgcccgact gcggcagatg atggcaagcc
     4561 ccgaatacgg taacgcggcg tggaataccg cttttgccac aaccctgcgc gaccacgcgc
     4621 gctttgaaga acgcgagctg tttcccgccg ccgaaccgtt tttgccggca tgattccgtt
     4681 ttgcggcaaa tatattaatg ataaacaagg aacacacatg aaatttacca agcaccccgt
     4741 ctgggcaatg gcgttccgcc cgttttattc gctggcggct ctgtacggcg cattgtccgt
     4801 attgctgtgg ggtttcggct acacgggaac gcacgagctg tccggtttct attggcacgc
     4861 gcatgagatg atttggggtt atgccggact ggtcgtcgtc gccttcctgc tgaccgccgt
     4921 cgccacttgg acggggcagc cgcccacgcg gggcggcgtt ctggtcggct tgactatctt
     4981 ttggctggct gcgcggattg ccgcctttat cccgggttgg ggtgcgtcgg caagcggcat
     5041 actcggtacg ctgtttttct ggtacggcgc ggtgtgcatg gctttgcccg ttatccgttc
     5101 acagaatcaa cgcaactatg ttgccgtgtt cgcgctgttc gtcttgggcg gcacgcatgc
     5161 ggcgttccac gtccagctgc acaacggcaa cctaggcgga ctcttgagcg gattacagtc
     5221 gggcttggtg atggtgtcgg gttttatcgg tctgattggt acgcggatta tttcgttttt
     5281 tacgtccaaa cggttgaatg tgccgcagat tcccagtccg aaatgggtgg cgcaggcttc
     5341 gctgtggctg cccatgctga ccgccatgct gatggcgcac ggcgtgatgc cttggctgtc
     5401 ggcggctttc gcgtttgcgg caggcgtgat ctttaccgtg caggtgtacc gctggtggta
     5461 taagcctgtg ttgaaagagc cgatgctgtg gattctgttt gccggctatc tgtttaccgg
     5521 attggggctg attgcggtcg gcgcgtctta tttcaaaccc gctttcctca atctgggtgt
     5581 gcatctgatc ggggtcggcg gtatcggcgt gctgactttg ggcatgatgg cgcgtaccgc
     5641 gctcggtcat acgggcaatc cgatttatcc gccgcccaaa gccgttcccg ttgcgttttg
     5701 gctgatgatg gcggcaaccg ccgtccgtat ggttgccgta ttttcttccg gcactgccta
     5761 cacgcacagc atacgcacct cttcggtttt gtttgcactc gcgcttttgg tgtatgcgtg
     5821 gaagtatatt ccttggctga ttcgtccgcg ttcggacggc aggcccggtt gagacaaacc
     5881 gccgcagatt tcgggtctgg gcggtttgct tttcagacgg cagggcggtc agttgccgtc
     5941 cagccagcgg tcgcgtgtgg ttttggcctc ttcaaaatag cggtacaggg cttcgcggtc
     6001 gtcggtggtc aggatgtttg ccaaaacgtc caactgtttg cccaagcctt gaaccagttg
     6061 cagcaggctg tctttgttgg caaggcagat gtccgcccac acggcgggat ggccggaggc
     6121 gatgcgggtg aagtcccgaa agcccgtggc ggcgaatttc agatattcct gtccgtcggg
     6181 gtggtcgaga atctggtgga cataggcgaa ggcggtcagg tggggcatat gggagacggc
     6241 ggcgaaaacc gcgtcgtggc gttgcgcgtc catcgtataa atttccgcac cgaccgcgtg
     6301 ccacaggttt tctatcaagg caatgccgtc tgaatgttcg ccgccgtgtg gcgtgatgat
     6361 gagttttctg tggcggaaca gcccgaactg cgcggcttgc gcaccgcttc tgtccgaacc
     6421 ggcaattggg tgggcggcga tgcagtggtg caggcggtcg ggcagacagc ggcggaaggc
     6481 ttcgatgacc gaagatttgg tgctgccgac atcggaaatc caagtgtgtt ccggcaaaac
     6541 ggggcgcagc gcggtcaaaa tggcgggaac ggtggcgacg ggcgtggcaa tcagtaccaa
     6601 gtccgcaccg ccgatgctgt ccgcgtcgat ggcaacggaa gcccggtcaa tcacgccgcg
     6661 ttccaatgca cgttcgaggt tgtcgcggtc ggtgtcgata ccggtaacgg tgcggacgag
     6721 tccctgcctt ttgaggtcga gaacgaacga accgccgatc agccctacac cgatgagggc
     6781 aatatggttc aaaatgggca tttgtgtaaa cggttttcgc aaagtaccgt catggtagcc
     6841 tatcggcgga atatgccgca aggtcggcag gaaaaaggag aagaaatgga caaaatcaga
     6901 gttgccgccg tgcagatggt gtcgggcgtg tcgccggaaa ccaacgtcgc tgccatgaaa
     6961 cgcctggtcg cacgggcggc ggagcagggg gcggattggg tgctgctgcc cgaatattgg
     7021 gtgctgatgg gcgcaaacga taccgacaaa ctcgcgcttg ccgagccttt gggcggcgga
     7081 cgctttcaga cggcattgag cgaaacggcg aaagaatgcg gcgtggtgct gtttggcgga
     7141 actgtgccgc tgcaaagcag tgaggcaggc aaggtgatga atacactgtt ggtgtacgga
     7201 cgggacggcg taagaacggg tctgtaccac aaaatgcacc tcttcggctt ttccggtttg
     7261 ggcgaacgct atgccgaagc cgataccatc agcgcagggc gggcagtgcc gcacttgtcg
     7321 gcagagggcg tgccggtggc ggcaggtatt tgttacgatg tccgctttcc cgaatttttc
     7381 cgccgccagc tgccgtttga cgtattgatg cttccggcgg catttaccca tacgacgggc
     7441 aaggcgcact gggagctgct tttgcgtacg cgtgccgtcg aaaaccaatg ttatgttgtg
     7501 gcggcggcac agggcggatt gcacgaaaac ggcaggcgca cgttcggaca cagtatgatt
     7561 gtcgatccgt ggggcgacgt gttggacgta ttgcccgaag gcgaaggcat cgttacggca
     7621 gacattgatg ccaaccgcct gaacagcgtc cgcaaccgcc tgcccgccct gaaataccgg
     7681 gttttggatg ccgtctgaag gttcagacgg catcggtgcc ggggaatcag cgggctgtag
     7741 gatgggcttc agcctgccat ttccatcaat cccacatttc taccgttttc atcgaatcca
     7801 tcgaatccgc ccctttcgac gacccagtcc tacgcaactg aaccgtcatt cccacgga
//
LOCUS       DAWHRS010000097         7798 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWHRS010000097 DAWHRS010000000
VERSION     DAWHRS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.097"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(452..2488)
                     /locus_tag="WIB82_001486"
     CDS             complement(452..2488)
                     /locus_tag="WIB82_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ3842772.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTDWANTVERLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAV"
     gene            complement(2576..3448)
                     /locus_tag="WIB82_001487"
     CDS             complement(2576..3448)
                     /locus_tag="WIB82_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ3842773.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYVEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3718..6108
                     /gene="gyrB"
                     /locus_tag="WIB82_001488"
     CDS             3718..6108
                     /gene="gyrB"
                     /locus_tag="WIB82_001488"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ3842774.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIETNEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            6205..7590
                     /locus_tag="WIB82_001489"
     CDS             6205..7590
                     /locus_tag="WIB82_001489"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ3842775.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPSGLNVRRRVPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7661..7759)
                     /locus_tag="WIB82_001490"
     CDS             complement(7661..7759)
                     /locus_tag="WIB82_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ3842776.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         1770 a         2155 c         2204 g         1669 t
ORIGIN      
        1 cgtctgaagc gttgtttgac cgtttcagac ggcattttta tcaaattcaa aaatattccg
       61 tcattcccgc gaaagcggga atctagaacg taaaatctaa agaaaccgtt ttatccgata
      121 agtttccgca ccgacagacc tggattcccg cctgcgcggg aatgatggga tttctgtttt
      181 tgattttttt gtttttgggg aatgacggga tttgagattg cgggcattta tcgggtaaaa
      241 cggaaattaa acgttgcgaa aatttatccg gaatcacagc aacttttccg cgtcattccc
      301 acgaaagtgg gaatctagaa cgtaaaatct aaagaaaccg tttttcccga taagtttctg
      361 caccgaccgg tctggattcc cgcctgcgcg ggaatgacgg tgcggatgtt tttctatcga
      421 atccgccata ttttttactt caaccctgcc gtcagaccgc attgtcgaaa ccgctgtggc
      481 gcaagagtgc gtctatgctc ggttcgcggc cgcggaaggc tttgaaggat tctgccgcgc
      541 tgcgcgatcc gccgacggcg aggatttcct gccaaaagcg tttgcctgtg gcggcgacat
      601 cgtcgctttc ttcaaaggcg gcgtatgcgt ccgcgctcaa tacttccgcc cacgcgtagc
      661 tgtaatagcc tgcggaatag ccgcctgcga agatgtggcc gaagctcaag gcgaagcggt
      721 tgtattcggg cggctggatg acggcgactt ttttgcgcac gctgtctaaa acctgttgcc
      781 agtttttcag acggccttcg tcgtcttcgc tgtaaatcat catatcaaag agggcgaact
      841 ccatttgccg gacgaggaac atgccgcgtt ggaagttttt ggcggcgagc attttgtcga
      901 agagttcttt cggcagggga acgccggttt cttcgtgggc ggacatttgt gccaagacat
      961 tgtattccca aacgaaattt tccataaact ggctgggcag ttcgaccgcg tcccattcta
     1021 cgccgttgat gccggatacg cccagttcgt ccacttgggt aagcaggtgg tgcagcccgt
     1081 gtccggtttc gtggaagagg atgaggattt cgtcgtggct caggcgggct tccctgccgc
     1141 cgacgggtgg ggcgaagttg cagacgaggt aggcggtggg cagttgcagc gtgccgtctg
     1201 aaaaacggcg gcggcctttg tagtcgttca tccacgcgcc gccgcgtttg ccttcgcgtg
     1261 cgtacaaatc catataaacg ccgcctatgg tttcgccgtt ttgttgcaat tcaaaatagc
     1321 gcacgtcttt gtgccagacg gggacggttt tttcggtaaa tccgatgccg tagagttttt
     1381 tgatttgggc gaacagtccg tttaatactt tgccgacggg gaagtatttt ttgacttcgg
     1441 tttcgctgaa cgcgtatttg gcttcgcgca gtttttcgct ggcgtagccc aagtcccacg
     1501 gttgcaaatc ggcgaggttc aggctttcgc gggcgaaggc tttgacttcg gcgaggtctt
     1561 tttcggcgta gggtttggcg cggcgggcga ggtcgtgcag gaagtttaaa acttgttcgg
     1621 gcgtgtccgc cattttggtt gccagcgaca attcggcgta gtttttgaag ccgagcagtt
     1681 tggcggtttg cagggcgttt gcgagcgtgc ggtcgatgtt ggcggtgttg tcgaatttgc
     1741 cgtcgtctga aagttcgctg gcgcgggtaa cgtaggcgcg gtagatttgt tcgcgcagtt
     1801 cgcggttgtc ggcgtattgg atgacggcga ggtagtgtgg aatctgcaag ccgattttgt
     1861 agcctgtttt gctttcgctt tgcgcggcgg cggcaaacat ggcgagcgcg tcttcgggaa
     1921 tgccggcaag cggtgcggca tcgtcaaagt aaatgccgaa cgcgtcggtc gcgtctagga
     1981 cgttttggga gaatttggcg gaaagttgcg cgccttcggt ttgcagtttt gccagttctg
     2041 cctgctgttc gggcggcagt tccgcgccgc tgaggacgaa atcgcgcaga tcgtggttga
     2101 gtttggtttt ttgtgcgggg gagagggtgt cgaattcggg ggaatttttg atggttttga
     2161 agcggttgta cagctcgatg tcttgtccga tttcggtgaa gaagacggtg atttcgggca
     2221 tcagttcgtt atagacggcg cgcagttcgg gcgtgtcggc gacggagttg aggtgcgaca
     2281 ccacgcccca aatcctgccg acgcgctcgg tgatgccggt cagacgctcg acggtgttcg
     2341 cccagtcggt gtgtgtttgt gctttgatgg cggcgatttg ttcgcgcgct tcggcaatgg
     2401 cggtttgcag ggcgggtttg atgtcttcgg ttttgatttg atcaaaacgg ggttcttcgc
     2461 ccaaatggag cagtgcgttg tcagtcataa gatgggtttc ctttgcgtgt tgtgcttcag
     2521 acagcatatc ggcaatatgc cgtctgaagc cggaaatggg gtcggtcggg gaaagttaaa
     2581 gtgtttcgga gccggcgggg taatttaaca ggcggtattg tacgcgcaaa aattcgtcca
     2641 taaccgcctg ttggatttcc agccgctttg aaacggggga ggcgaagcgg acgatgatgc
     2701 ggtatgcctt gtcatcgtac ggcacgcggg taacgcgcgg tctggcggcg ggcgtgataa
     2761 acagtttttc cgcctgcacg ttttccaaat gccgttggat ggcggggatg tagggcgcgc
     2821 acaagggctc gagtacggct ttcagacggc atacggcttc atccgaatcc aaatggatgg
     2881 gaacggggat ttcgaccgta tggatgacat agtcgcccaa aatattgtcg cggcgcacgg
     2941 ggtggctcaa caacaggctg ttggggaaag aaacggtggt tcccgcaagc tgtccgacca
     3001 aggggttcgg accgacctgc atcatcagcg tgttcaacag gttgatgtcg accacgcgcc
     3061 cgcgcaggcc gttgatttcg acatagtcgc cgaccgagta ttgctgggtg gcagacctta
     3121 aaatactgcc cgacagacac ataatcagtt ccttcgtcgc cacgacgacc gccgccgcca
     3181 ccgcaaacat cgacaaagcc agcgtttgga tttgcgccga ccagataaat gccagcgaaa
     3241 acagcaccaa aagcagcgtt atattgcggc tggcaaccaa aaaccgccgc ttgctttcga
     3301 tgccgaaatc cggatgccgt ttgaagtgga tattcaacag aagggcgcgc gccagcagca
     3361 aagccgcaac cgccgccacg gattcgaccg cctccgcacg tatcgggacg gcaccgagcc
     3421 aagtgtccaa catattccat atttccattt ccgcgcccct gtccgaaaag tatagggtgt
     3481 agattttagt ggcaaaaaaa cgcttttgcc actttacggg acaatccccg acctgatgcc
     3541 ggaggcaaac gtaccgtttc atcttttccc ggcggcacac tgatgcggat aacgctgaat
     3601 taggcggcaa aatcggctat aatacacaat tatatatgtc gtcccgcccg ctttttctac
     3661 aaggtgcgga cttgttttgg cggcatcgca aaatcttttc aaaatccggc aaaaaatatg
     3721 actgaacaaa aacacgaaga atacggcgcc gacagcattc aggtgctcga aggcttggaa
     3781 gcggtacgca aacgccccgg catgtacatc ggcgacacgc aggacggcag cggtctgcac
     3841 cacatggtgt tcgaagtatt ggacaacgcc attgacgaag cactcgccgg acattgcgac
     3901 aaaatcacgg taacgataca cgccgaccat tccgtcagcg tcgccgacaa cgggcgcggt
     3961 atgcccaccg gcatccaccc gaaagaagga cgctccgccg ccgaagtcat catgaccgta
     4021 ttgcacgcgg gcggtaaatt cgacaacaac agctacaaaa tctccggcgg cctgcacggc
     4081 gtgggcgtgt ccgtcgtcaa cgcgctgtcc gactgggtaa cgctgaccat ctaccgcgac
     4141 ggcaaagaac acttcgtccg cttcgtgcgc ggcgaaaccg aagagccgct gaacgttgtc
     4201 ggcgattccg ataaaaaagg cacgaccgtg cgcttcctcg ccagtacgga aaccttcggc
     4261 aacgtcgaat acagcttcga catccttgcc aaacgcatcc gcgaactttc gttcctgaac
     4321 aacggcgtgg acatcgaatt gaccgacgag cgcgacggca aacacgaaag cttcgccctt
     4381 tccggcggcg tggctggatt cgtgcaatac atgaaccgca aaaaaacgcc cttgcacgaa
     4441 aaaatcttct acgcgttcgg cgagaaagac ggcatgagcg tcgaatgcgc gatgcaatgg
     4501 aacgacagct atcaggaaag cgtgcagtgc ttcaccaaca acatccctca gcgcgacggc
     4561 ggtacgcacc tgaccgcgtt gcgccaagtg atgacgcgca ccatcaacag ctacatcgaa
     4621 accaacgaag tcgccaaaaa agccaaagtg gaaaccgccg gcgacgatat gcgcgaaggt
     4681 ttgacctgcg tgttgtccgt caaactgccc gaccccaaat tttcgtccca aaccaaagac
     4741 aaactggttt ccggcgaaat cggtcccgtc gtcaacgaag tcatcagcca agccctgacc
     4801 gacttcctcg aagaaaaccc tgccgaagcc aaaatcatca ccggcaaaat cgtcgatgcc
     4861 gcccgcgcgc gcgaagccgc ccgcaaagcc cgcgaaatca cccgccgcaa aggcgtgatg
     4921 gacggtttgg gcctgccggg caaactcgcc gactgccaag aaaaagaccc cgcattgtca
     4981 gaactttatc tggtcgaggg cgattctgca ggcggttccg ccatgcaggg gcgcgaccgc
     5041 aaattccaag cgattttgcc gcttaagggc aagattttga acgtcgaaaa agcgcgcttt
     5101 gaaaaaatgc tcgccagtca ggaagtcgcc gctttgatta ccgcgttggg cgcgggcatc
     5161 ggcaaagaag aattcaatgc cgagaagctg cgttaccacc gcatcattat catgaccgat
     5221 gccgatgtgg acggtgcgca tatccgcacc ttgcttttga ccttcttcta ccgccaaatg
     5281 cccgaactgg tcgagcgcgg ctacatctat atcgcccagc cgcctttgta taaagcgaaa
     5341 tacggcaaac aggaacgtta cctcaaggac gagttggaaa aagaccaatg gctgctcggt
     5401 cttgccttgg aaaaagccaa aatcatttca gacggccgca ccatcgaagg cgcagaactt
     5461 gccgacaccg ccaaacaatt cctgttggca aaaaccgtca tcgaacagga aagccgcttc
     5521 gtagacgaac tcgtcctgcg cgccatgcta cacgcgtcgc ccattgattt gacgtcgtct
     5581 gaaaacgccg ataaagccgt tgccgaactt tccggtctgc ttgacgaaaa agaagtcgcc
     5641 ctcgaacgca tcgaaggtca tgaaggacac cggttcatca aaatcacgcg caagctgcac
     5701 ggcaacgtca tggtcagcta catcgaaccc aagttcctca acagcaaagc ctaccaaacc
     5761 ctcacccaaa ccgccgccgc gctcaaaggc atggtcggcg agggtgccaa gctttacaaa
     5821 ggcgaaaacg ggtacgacgc ggacagcttt gaaaccgctt tggacatctt gatgagcgtt
     5881 gcccaaaaag gtatgtccat ccaacgatac aaaggcttgg gcgagatgaa ccccgagcag
     5941 ctgtgggaaa ccacgatgga tcccgccgtg cgccgcctgt tgaaagtgcg catcgaagat
     6001 gccattgccg ccgacgaagt gttcgttacg ctgatgggcg acgaggtcga gccgcgccgt
     6061 gcctttatcg aaaacaacgc gctgattgcc caaaatatcg acgcataagt gccgttttaa
     6121 aaaaggagac gggcatcgtg ccgcgtctcc tttttggttg gtcaaacgga acctgtgccg
     6181 tctgaaaaac cgtcggagca aaatatgatc agcattttcg atattttcaa aatcggtatc
     6241 gggccttcca gttcgcatac ggtcggcccg atgaaggcag ccgccgcctt tgcggcaggt
     6301 ttggatgcac aggctgttcg catcgtcatc gacatttacg gctcgctcgc actgaccgga
     6361 tacggacacg gtacatttga cgcgctgatg ctcggtttgg aaggcagcct gccgcacgac
     6421 atcccgcttg ccggcattcc cgaacgcctc gaacgcatcc gcacgcagca catcctccgg
     6481 ctcaacgggc aagaaatccg cttcatcccc gaccgcgacc tgaacatact cggcaatcaa
     6541 gtgctgccca aacaccccaa cagcctgcgt tttaccgcct atgcttcaga cggcacggta
     6601 ttgaatgaac aggtttatta ttcggtcggc ggcggctttg tcgttaccga agaagatttt
     6661 gaccggcagg cggaaacgga aaaagccgtt ccctatccct ataccagttg cgccgaactg
     6721 cttgcccgat gccgtctgaa ccggctcgac atctccgaag tcgtgttggc aaacgaagcc
     6781 gcgcttgccg gatgcggcga agccgaaatc cgccgccgcg ccgctgccgt tgccgaggtt
     6841 atggaaggct gcatcaaacg cggcttgggt gcggacggcg aactgcccag cggattgaac
     6901 gtccgccgcc gcgtcccgca gcttgccgcc aagctcaaag tcctgcgcga aaccgaaatc
     6961 gtcaacaccc agctctggcc gatggtgtac gccatggcgg tcaacgaaga aaacgccgcc
     7021 ggcggacgcg tcgttaccgc accgaccaac ggcgcggcag gcatcattcc cgccgtattg
     7081 cactatttcc gcaagttcaa tccgcacgcc acacaggaac gcgtcgaaaa cttcctgctc
     7141 accgcaggcg caatcggcat cctctacaag accaacgcct ccatttccgg tgcggatgtc
     7201 ggctgtcagg gcgaagtcgg cgtagcgtgt tcgatggcgg cgggcgcata cgccgaagtc
     7261 atcggcggca cgcccaaaca agtggaaaac gccgccgaaa tggcgatgga acaccatttg
     7321 gggctgactt gcgaccccgt cggcggactg gtgcaaatcc cctgcatcga gcgcaacggc
     7381 atcgccgccg aaaaagccct caaactcggc acgctcgcgc ttttggaaga cggcacggac
     7441 aaaaaagtct cgctcgacga agtcatccga accatgctgc aaaccggccg ggatatgaag
     7501 gcgacctaca aagaaacctc gcttgccgga ctcgccgcca cgctccggaa aaaagccgtc
     7561 cccgtatccg tgcgcgtggt cgagtgctga ggatggacag aaacgaaaat gccgtctgaa
     7621 aacagttttt tagacggcat ttcatttatt tcttgcggct ttaccgtttc agtgccagcg
     7681 tcaacacgcc cgccgtaacc agccccaagc ctatccattc ctgcgtgttc gggcgttcgt
     7741 ctaagaaaac caccgccatc aggaatgcaa accggcgttg tttgcagcat ccggcaag
//
LOCUS       DAWHRS010000098         7782 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWHRS010000098 DAWHRS010000000
VERSION     DAWHRS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.098"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(31..447)
                     /gene="dksA"
                     /locus_tag="WIB82_001491"
     CDS             complement(31..447)
                     /gene="dksA"
                     /locus_tag="WIB82_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ3842777.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(594..1385)
                     /locus_tag="WIB82_001492"
     CDS             complement(594..1385)
                     /locus_tag="WIB82_001492"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ3842778.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIYIANRGAEKRERLEKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNICTNGALVLSVAAGLSVDTLSRYLGG
                     SRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDCKIADRIMKSVGLTVWLEDEVQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1434..1841)
                     /locus_tag="WIB82_001493"
     CDS             complement(1434..1841)
                     /locus_tag="WIB82_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ3842779.1"
                     /translation="MKIRAIKYTAMAALLAFTVAGCQLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1841..2527)
                     /locus_tag="WIB82_001494"
     CDS             complement(1841..2527)
                     /locus_tag="WIB82_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ3842780.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYASGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2647..3690
                     /locus_tag="WIB82_001495"
     CDS             2647..3690
                     /locus_tag="WIB82_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ3842781.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3853..5079
                     /locus_tag="WIB82_001496"
     CDS             3853..5079
                     /locus_tag="WIB82_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ3842782.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5365..7515
                     /gene="yccS"
                     /locus_tag="WIB82_001497"
     CDS             5365..7515
                     /gene="yccS"
                     /locus_tag="WIB82_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ3842783.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQENVANAYEALGSYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETSSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVETLNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKIAERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1939 a         2586 c         1676 g         1581 t
ORIGIN      
        1 gggactcaga cggcattttg tttgccgcca tcagtcggca aactgttttt tcatcctctc
       61 tcggcgttct tgggactcaa cagataaagt ggctgtcggg cgtgccagca gccgcttcag
      121 accgataggc tctcccgtat cggcacagaa tccataatcc ccttcatcaa tattgcggat
      181 ggtcgcctgt attttactga gaagttttcg ttcccgatcg cgggtacgga gttccaatgc
      241 gtactcttct tcctgtgtgg cacggtcggc aggatcgggg gctgattcgt gttcttggag
      301 atgccctgtc gtagcggaag cattttcgat gagttcgtct tgcattttta ccagcaattc
      361 gcggaaaaaa gccaaatggt cgtcattcat ataatcgtct tccggcccgc tccaattcaa
      421 aatatcttgt tctgtcagct ttgccataat gtatatcctc tcggttcacc gaaaaaaaca
      481 aagcaccaca aagtggcttt attcctttga tttttataag gcttattgcc taaggcgttt
      541 ttgaaaacgc gcatcataac acgttttgga ttggtttttt tattttcttt acattattga
      601 tattgccgtt ccatttcctg cgaacggcgc acacaggcac aaacgccctc acttatggct
      661 tcggcgacac gatgccgcct gaaggcttcc acggcttcgt gggttgtccc gccttttgac
      721 gttacatttt tttgaagctt ctcgaaatct tcacccgtct gctcggcaag ggcaaccgct
      781 cctttaaacg ttgccagact gagcgcgcgt gcttctgcca tatcaaaccc ttgtcggatg
      841 gcggcatttt gcaaagcatc cagcagataa aacacataag ccggcccgct gccgctgatg
      901 ccggtgatgc tgtgcatttg cacttcatct tccaaccaaa cggtcaaacc gacagatttc
      961 atgattcgat cggcaatctt gcagtctgtt tccgatactt ccgcttcggc atacataccg
     1021 gagacaccca gtcctatttt cccgggtgta ttcggcataa cccggacaat gcggcggctt
     1081 cccccgaggt aacggctgag cgtatcaacc gacaatccag ctgcaacaga aagcaccaat
     1141 gcgccgttgg tgcagatatt tttgcacgcg gcttccatat cctgcggttt gacggcaagg
     1201 attaaaacat cgtcggaatg aagctccggc agggttgccg aagtttcgac ccccaactct
     1261 ttttccaaac gttcgcgttt ttccgcaccc cgattggcta tatagatgcg gtaaccgcct
     1321 tgtttgacca atccgcccgc aacggcagcc gccatattgc cgccgccgag aaaataaaca
     1381 ttcattattt ctaccctatt caaaattacc tgactaaatt cattcccaat tttttaccat
     1441 cggacgcgct cggacagacc gttgcgccgc aacccctgct tttccaagca gtcaaatcct
     1501 tcctgtttga acgtctcaat caaaggtttg ccgtcacgcg tacgccaatc caacccgcag
     1561 gcttcaaact tccctatttt cctgtaaaag taagattgtt gtgcggattc atattcgttt
     1621 gccctgacgg aacgattgcc gtctgaaagc ggtatctcgt agtcctctaa agacggcgga
     1681 ctctcgccgc caatgtccca aaaatcgatg gcagccggtt ttctcggctt acaccagccg
     1741 gacagggacg aacactcata ccaacccgcc aactggcagc ctgcaaccgt aaatgcaagc
     1801 aacgcagcca ttgccgtata ttttattgcc cgaattttca tcagcccctt ttcccgaaaa
     1861 tcgcgctgcc gatacggacg tgtgtcgcac cgcactcaat ggcggcaggc atatcgtccg
     1921 acatccccat agacagcacg tctgccttaa cgccagccgc attgaggtcg gcaagcagtt
     1981 tccgcatcgt ttgaaattgc accttcaact ccgtttcact gctgttggct ttggcaacac
     2041 acatcagtcc gcgtacgacg atattcggca gcttcgccac ttccacagca agcgcgactg
     2101 cttcttcggg cgcgacaccg tgcttcaccg cctcgcccgc aatgttcacc tcaatacaca
     2161 cctgcaaagg cggcattgag gaaggacgtt gcccgctcag ccggacggcg gtctttagac
     2221 ggcatacggt atgcacccaa tgcgcgcgtt cggcgacaaa cttggttttg ttggactgca
     2281 catcgccgat gacgtgccac acgatgtcgg tcaaatccgc caactcttcc gttttgccgt
     2341 accactcctg aatatagttc tcgccgaaat cacgctgtcc tgaggcgtaa acttcgcgga
     2401 tgccgtctga agggaaagtc ttaccgacgg caatcaggct gacggaatgc ggcttccttc
     2461 ccgcctgcag aaccaatttt ccgatacggt cggacacctc ccgataacgt tcttgcaaca
     2521 ccgtcataga ttatccccta attaaaaatg attaaacagt tgaaacccct ccagtcaggg
     2581 cggtacaatc aaggttgtta gaaccattcc aaccaatcga aacattatac taaacagagc
     2641 cgcattatgc agattaccga cttactcgcc ttcggcgcca aaaacaaagc atccgacctt
     2701 cacctgagtt cgggcatatc ccctatgatt cgagttcacg gcgatatgcg gcgcatcaac
     2761 cttcccgaaa tgagcgcgga agaggtcggt aatatggtaa cttcggtaat gaacgaccac
     2821 cagcggaaaa tctaccagca aaacttggaa gtcgacttct cgttcgaact gcccaacgtc
     2881 gcccgattcc gcgtcaacgc cttcaacacc ggccgcggtc ccgccgccgt attccgcacc
     2941 attcccagca ccgtcttatc gctggaagaa ttgaaagccc cgagcatttt ccaaaaaatc
     3001 gcagaatcgc cgcgcggcat ggtattggtt accggcccta ccggttcggg caaatcgacc
     3061 acgcttgccg cgatgatcaa ctacatcaac gaaacccagc cggcacacat cctgaccatc
     3121 gaagacccga tcgaattcgt ccaccaaagc aaaaaatccc tgattaacca gcgcgagctg
     3181 caccagcaca ccctcagctt cgccaacgcg ctgagttccg cattgcgcga agaccccgac
     3241 gttatccttg tcggcgagat gcgcgacccc gaaaccatcg gcttggcact gaccgccgcc
     3301 gaaaccggac acttggtttt cggcacgctg cacacgaccg gcgcagcaaa aaccgtcgac
     3361 cgtatcgtgg acgtattccc ggcgggagaa aaagaaatgg tgcgctctat gctgtccgaa
     3421 tcgctgaccg ccgtcatctc ccaaaacctg ctgaaaacgc acgacggcaa cggccgtgtc
     3481 gcctcgcacg aaatcctgat tgccaacccc gccgtccgca acctcatccg cgaaaacaaa
     3541 atcacgcaga ttaactccgt cctgcaaacc gggcaggcga gcggtatgca gacaatggac
     3601 caatcgctgc aatcgctggt gcgccaaggg ctgatcgcac cggaagtcgc acgcagacgc
     3661 gcgcaaaaca gcgaaagtat gagtttctga cacacaaccg ccttccggcc ataccggcgg
     3721 gaaaacaagg cgcaaacacg cgaggcggga cgcagcatcc cgcccggcta cctttccgaa
     3781 caagaagcgt ccgccttcct gttgaaacct gccgccgcaa actgcaaggc ttaaaccgaa
     3841 aagaagctaa cgatgaatac cgataacctg cacgacatct tggacgaaat ggttcaagtg
     3901 tattctcaaa aaaaacaaag ccgatccgaa accccggccg aaatcggcgc acacttccac
     3961 ccgctgctcg accgcctgtg cgaaaccgca gaagcacaaa acgcgtccga catccttatc
     4021 agcaaaggat tcccgccctc gttgaaaatc aacagcgcat taaccccgca gccgcaaaag
     4081 gcgctgacag gcgaggaaac cgccgccatc gccgcatcga cgatgaacgc cgaacaatcg
     4141 gaaatattcc ggcgcgacgg cgaaatcaac tactccgtcc agtcgcgcag cggcacgcgc
     4201 taccgcgcca acgcctacca cagccaaggc agcgcaggtt tggttttgcg gcgcatcaac
     4261 cacgtcatcc cgcaaatgca ggaattgggc ctgcccgaaa aactcaaaga cctcgccgtc
     4321 gcaccgcgcg ggctgctgat tatcgtcggg cctaccggtt cgggcaaatc caccacgatg
     4381 gcgactatgc tcgaacaccg caacaaaacc ctgcccagcc atatcgttac catcgaagac
     4441 ccgattgagt ttatctacaa accgcgccgc tgcatcttta cccagcgcga aatcggcgtc
     4501 gacaccataa actggcagac ggcggtacaa aacgctatgc gccaatcccc cgacgtggtc
     4561 tgcatcggcg aagtccgcag cagggaaagt atggaatacg cgatgcagct cgcccaaacc
     4621 ggccacctgt gcatttttac gctccacgcc aacaccgcgc cgcagtcgct cgaacgcata
     4681 ctcaacttct accccaaaga acagcacaac caaatactga tcgacatcgc cctcaacctg
     4741 accggcatca tctgccaacg cctcgccctc aaacaagaca aaacgggcag gacggcggtt
     4801 gtcgacttgc tcatcaacac gcccgccatc caagacttca tcctgaaggg cgacctgatg
     4861 aacatcagta aaatcatgga aaccgccaaa accgacggaa tgcagacgat ggatcaaaac
     4921 cttttcgaac tgtaccgtca cggcatcatc agttacgaag aagccctgcg ccagtccgtt
     4981 tccgccaaca acctgcgatt gcacatccaa ctgcacaaag aaggcaaaac gcccgaactc
     5041 ctttacgaca gggtcaacgg tctcaacctc atttcctgat ccgcaaaacc caatgccgtc
     5101 tgaaaaccgc atccccgttt tcagacggca tgattttatc cgccccattc atgtgctaca
     5161 ctttatagtg gattaaattt atagtggatt aacaaaaatc aggacaaggc gacgaagccg
     5221 cagacagcac aaatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt
     5281 tctctttcag ctaaggcgag gcaacgccgt actggttttt gttaatccac tatattcact
     5341 tcataacaat aaaccggtaa aaccatgaaa accccactcc tcaagcctct gctcattacc
     5401 tcgcttcccg ttttcgccag tgtttttacc gccgcctcca tcgtctggca gctaggcgaa
     5461 cccaagctcg ccatgccctt cgtactcggc atcatcgccg gcggccttgt cgatttggac
     5521 aaccgcctga ccggacggct gaaaaacatc atcgccaccg tcgccctgtt taccctctcc
     5581 tcgctcacgg cacaaagtac cctcggcaca gggctgccct tcatcctcgc catgaccctg
     5641 atgaccttcg gctttaccat tttaggcgcg gtcgggctga aataccgcac cttcgccttc
     5701 ggcgcactcg ccgtcgccac ttacaccaca cttacctaca cccccgaaac ctactggctg
     5761 accaacccct tcatgatttt atgcggcacc gtactgtaca gcaccgccat catccttttc
     5821 caaatcatcc tgccccaccg ccccgttcaa gaaaacgtcg ccaacgccta cgaagcgctc
     5881 ggcagctacc tcgaagctaa agccgacttt ttcgatcccg acgaagccga atggataggc
     5941 aaccgccaca tcgacctcgc catgagcaac accggcgtca tcaccgcctt caaccaatgc
     6001 cgttccgccc tgttttaccg ccttcgcggc aaacaccgcc acccgcgcac cgccaaaatg
     6061 ctgcgttact actttgccgc ccaagacata cacgaacgca tcagctccgc ccacgtcgat
     6121 tatcaggaaa tgtccgaaaa attcaaaaac accgacatca tcttccgcat ccaccgcctg
     6181 ctcgaaatgc agggacaagc ctgccgcaac accgcccaag ccctgcgcgc aagcaaagac
     6241 tacgtttaca gcaaacgcct cggccgcgcc atcgaaggct gccgccaatc gctgcgcctc
     6301 ctttcagaca gcaacgacag tcccgacatc cgccacctgc gccgccttct cgacaacctc
     6361 ggcagcgtcg accagcagtt ccgccaactc cagcacaacg gcctgcaggc agaaaacgac
     6421 cgcatgggcg acacccgcat cgccgccctc gaaaccagca gcctcaaaaa cacctggcag
     6481 gcaatccgtc cgcagctaaa cctcgaatca ggcgtattcc gccatgccgt ccgcctgtcc
     6541 ctcgtcgttg ccgccgcctg caccatcgtc gaaaccctca acctcaacct cggctactgg
     6601 atactgctga ccgccctttt cgtctgccag cctaactaca ccgccaccaa aagccgcgtc
     6661 cgccagcgca ttgccggtac cgtactcggc gtaatcgtcg gctcgctcgt cccctacttc
     6721 accccgtctg tcgaaaccaa actctggatt gtcatcgcca gtaccaccct ctttttcatg
     6781 acccgcacct acaaatacag tttctccacc ttcttcatta ccattcaagc cctgaccagc
     6841 ctctccctcg caggtttgga cgtatacgcc gccatgcccg tacgcatcat cgacaccatt
     6901 atcggcgcat cccttgcctg ggcggcagtc agctacctat ggccagactg gaaatacctc
     6961 acgctcgaac gcaccgccgc ccttgccgta tgcagcaacg gcgcctatct cgaaaaaatc
     7021 gccgaacgcc tcaaaagcgg cgaaaccggc gacgacgtcg aataccgcgc cacccgccgc
     7081 cgcgcccacg aacacaccgc cgccctcagc agcacccttt ccgacatgag cagcgaaccc
     7141 gcaaaattcg ccgacagcct gcaacccggc tttaccctgc tcaaaaccgg ctacgccctg
     7201 accggctaca tctccgccct cggcgcatac cgcagcgaaa tgcacgaaga atgcagcccc
     7261 gactttaccg cacagttcca cctcgccgcc gaacacaccg cccacatctt ccaacacctg
     7321 cccgaaaccg aacccgacga ctttcagaca gcactggata cactgcgcgg cgaactcgac
     7381 accctccgca cccacagcag cggaacacaa agccacatcc tcctccaaca gctccaactc
     7441 atcgctcggc agctcgaacc ctactaccgc gcctaccgac aaattccgca caggcagccc
     7501 caaaacgcag cctgaaaaag tttcggcatt ttgtaagaga gggcagattg tcagacaggt
     7561 tgcaagatag tggataaagc tttgcgcagg gtaaatgcgt agcaactgaa ccgtcattcc
     7621 cacgaatcta cattccgtca ttcccacgaa aacagaaaac caaaaacaga aacctaaaat
     7681 cccgtcattc ccacgaacct acattccgtc attcccacga aaacagaaaa ccaaaaacag
     7741 aaacctaaaa tcccgtcatt cccacgaacc tacattccgt ca
//
LOCUS       DAWHRS010000099         7730 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWHRS010000099 DAWHRS010000000
VERSION     DAWHRS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.099"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(16..771)
                     /locus_tag="WIB82_001498"
     CDS             complement(16..771)
                     /locus_tag="WIB82_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ3842784.1"
                     /translation="MKYIRLFFLCTALISTNAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYILHNLGFRCTSR"
     gene            complement(898..2058)
                     /gene="nirK"
                     /locus_tag="WIB82_001499"
     CDS             complement(898..2058)
                     /gene="nirK"
                     /locus_tag="WIB82_001499"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ3842785.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAASAEAASSAAQAAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMTMEDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAIAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2429..4575
                     /locus_tag="WIB82_001500"
                     /pseudo
     CDS             2429..4575
                     /locus_tag="WIB82_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
     gene            4754..5287
                     /locus_tag="WIB82_001501"
     CDS             4754..5287
                     /locus_tag="WIB82_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ3842786.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5350..6534)
                     /locus_tag="WIB82_001502"
     CDS             complement(5350..6534)
                     /locus_tag="WIB82_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ3842787.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNGGYAVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6574..6978)
                     /locus_tag="WIB82_001503"
     CDS             complement(6574..6978)
                     /locus_tag="WIB82_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ3842788.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEIVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            complement(7585..>7730)
                     /gene="rnhA"
                     /locus_tag="WIB82_001504"
     CDS             complement(7585..>7730)
                     /gene="rnhA"
                     /locus_tag="WIB82_001504"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="An endonuclease that specifically degrades the RNA
                     strand of RNA-DNA hybrids; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ3842789.1"
                     /translation="KNDDLWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAA
                     QFS"
BASE COUNT         1701 a         1889 c         2146 g         1994 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agatatattt
       61 ggattgcagg ctggtacgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccgata gacgcgccac tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagaccg tgcatatcat aaacacccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacattggt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcagac cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagctgccgc
      721 attggtactg atgagtgccg tacagagaaa aaataaccgg atgtacttca tatcatgcct
      781 cctgacaggc agttaaaatc aatcttccga aaggaaagat tggttgttaa aaaaccactg
      841 ccgtgcgtaa tgaagtacgg cggcagtggt tcgtttgcct aaaaatgggt gttcaattta
      901 gtaatcgctt tttgcaggag cagaggctgc aggagcagaa gctgcgggag cggaagcagc
      961 aggagctgca ccgttaccgg cgtaagcggt atcactcaat ttttgagtca tgatttcagg
     1021 gttttctgca ccctctactt tcaattgacc cagtgcgcct ttgttgaatg cgcggaagat
     1081 agagtggtca accaaagtgt agctgcccgg gatgtcgact ttgaattcga cgatggcaga
     1141 gccgccggca ggaacgatgg tgctttgtac gttttcgtta atcagtttgc cgccttccac
     1201 ataaactttg tcgaagattt caccgattac gtggaaggaa gacaccaagt taggaccacc
     1261 gttaccaacg tacatacgta cagtttcgcc tgctttggct ttcagggcgt tgtcgccggc
     1321 gatagcacct acgtgaccgt taaatacaac gtattcaggt tgttcggcga tggctttgtc
     1381 catatcgaac ggttgcaggc cttgcgcgcc ttttttgcct ttggtgtaga agtcgccttg
     1441 gacgatgtag aactctttat ccactttcgg cagaccttct ttaggctcga ccaaaatcag
     1501 accgtacata ccgttggcga tgtgcatacc gaccggcgcg acggcgcagt ggtagatgta
     1561 caggccaggt tgcagggctt tgaagctgaa tgtggaagta cggcccggag cggtaaaggt
     1621 tgcagccgcg ccgccgccct gaccggtagc cgcgtggaag tcgacgttgt gcggaacggt
     1681 agaagaagga ttgttggaga attccacttc aaccgtatcg ccttcgcgta cgcggatcat
     1741 gcggcccgga acatcgccgt caaatgtcca gtagcggtat tccacaccgt cttccatggt
     1801 catggttttt tcgacggttt ccattttcac gcggactttg gcagggtagt cgcggtcgat
     1861 tgcgggaggt acttcaggag cgtgggtggt aactgcatcg atgacgggca gttcgcctgc
     1921 aggcgtttcg gcggcagctt gtgcggcgga acttgcggct tctgcggaag cggcaggggc
     1981 ttgcgcggca ggttcgccgc cgcaggcggc taatgcgaat aaggaagcaa tcattgcagc
     2041 taaggcttgg cgtttcataa tattttcctt ttgtaagaaa agtagaggga ttggttaaga
     2101 acttggtgtg attatgcgtt tgccccattg ggggcatttt gatttaagtc aaataattca
     2161 taaaatatga acttaaaatg atacttctaa tactttatag ctactaaaaa ttacaataaa
     2221 taggctgatt tgcttaaggg cttcggcaaa tcaggcaaag gcagggtagt tgccgactaa
     2281 agaataatga tagttattat catatatgta tttgttttat atgtgagttt ctttgaacaa
     2341 atgtttattt gcgcggtaaa ccgtgctaca atctttaaca ttcatatttt gtgaatttta
     2401 atccactata ttaaaaggag ctctcaaaat gggacagtac aagaagctgt ggtacttgct
     2461 gtttgccgtt ctggcggtat gctttaccat tcttggctat atgggcagcg aggtttataa
     2521 gaaagccccg ccttaccccg aacaggtcgt ttccgcatca ggcaaagtgc tgatgacgaa
     2581 agacgatatt ttggcaggtc agtctgcgtg gcagagtacc ggcggtatgg aagtcggttc
     2641 gattctggga cacggcgcat atcaggctcc ggactggacg gccgactggc tgacgaatac
     2701 cgcaaccaaa gccgtgtgaa agaagacggc agcgtcgtca tcagcgatac gcgcgtaaaa
     2761 gctatcgaaa gcatcctgcc ttactaccac ggtgtttatg gcgacgatcc caagctgcaa
     2821 accactcgtg aacactttgc aatgaaaaac aatacattgc caagtcaaga ggcgcgtgaa
     2881 aaactgttca acttcttctt ctggacttct tggtctgctt cgaccaaccg tcctgacgag
     2941 aattttactt acaccaacaa ctggccgcac gaacctttga tcaacaacgt accgaccact
     3001 gaaaactata tgtggtcatt caccagcgtt gttcttctgc tgatgggcat cggtttgttg
     3061 atgtggggtt attccttcct gaccaaacac gaggaagtgg aagtgccgtc tgaagacccg
     3121 atttccaaaa tccaactgac cccttcgcaa aaagcattgg gcaaatacgt cttcctgacg
     3181 gtcgccctgt ttgtggtaca agtattgctg ggcggtctga ccgcgcacta caccgtcgaa
     3241 ggtcagggtt tctacggcat cgatgaggcg ttgggctttg aaatgtccga ctggttcccc
     3301 tatgccctga cccgtacttg gcacatccaa tccgccatct tctggattgc aaccggcttt
     3361 ttgacggcgg gtttgttcct cgcgccgatt gtcaacggcg gcaaagatcc caagttccaa
     3421 cgtgcaggcg tgaacttcct ctatatcgcc ctcttcatcg tggtcggcgg ttcttacgcg
     3481 ggcaacttct tcgcgttgac gcacatcctt ccccccgaat tgaatttctg gttcggacac
     3541 caaggttacg aatacctcga tttgggccgt ttctggcaac tccttttgat ggtcggtctg
     3601 ctgttatggc tgttcctgat gttgcgttgc acggtttccg ccttcaaaga aaaaggcgtg
     3661 gacaaaaacc tgttggctat cttcgtagct tctatggtcg gcgtgggcgt gttctacgcg
     3721 ccgggtctgt tctacggtga aaaatccccg attgccgtta tggaatactg gcgttggtgg
     3781 gtgtttcacc tgtgggtaga aggcttcttc gaagtatttg ccactgccgc ctttgccttt
     3841 gtcttctaca atatgggctt tgtccgccgc agtaccgcca ctgcctccac tttggccgct
     3901 gccgccatct ttatgttggg cggcgttccg ggcacgctgc accacctgta cttctccggc
     3961 tctacctccg cctctatggc aatcggtgcc tgcttctccg ctttggaagt cgtgccgctg
     4021 gtgttgctgg gccgtgaagc atacgaacac tggtcttacc aacacctgtc cgactgggcg
     4081 aaacgcctgc gttggccgct gatgtgcttc gtggcagtcg ccttttggaa tatgatcggt
     4141 gccggcgtat tcggcttcct gatcaatccg ccgatttccc tgttctatat ccaaggtttg
     4201 aacacttctg ccgttcacgc gcacgccgcc ttgtttggtg tgtacggttt cttggcattg
     4261 ggcttcgtat tgctggttgc ccgttatttg aaaccgaacg cgcagtttga cgacaaactg
     4321 atgacttggg gcttctggct gctcaacggc ggcttggtcg gtatgattgc catcagcctg
     4381 ctgcctgtcg gcgcgattca ggcatacgcc tccatcacgc acggtctgtg gtatgcccgc
     4441 agcgaagagt tcctgcaaat ggaaatcctc gataccttgc gttgggtacg tacggcagct
     4501 gacttggtct ttatcggcgg tgcaatctgc gttgccatcc aagctactaa aatcgtattc
     4561 ggccgcgaca aataagtttt gcggtatgaa atgagcaact gcacccgttt tcgggtgcag
     4621 ttttttattg cggattggga cgggaatgga aaaggatgtg gattttttat tttaatcggc
     4681 tatacaattg ctgctttcat gccgtctgaa gcacgtttca gatggcatcg gtttacatca
     4741 gataaggata aaaatgggta tttacgattt tcaaatgaaa gatgcggaag gcaatgcggt
     4801 tgatttgtca ggctatcgcg gcaaggttct gctgattgtc aacacggcaa cgcgttgcgg
     4861 tctgaccccg caatacgagg ctttgcagaa gctgtatgca caatataccg cagaaggctt
     4921 ggagattttg gattttccgt gcaaccagtt ccgcgaacag gctcccgaaa gcagcggaga
     4981 aattgcccaa gtgtgtatga tgaagtttgg cacgaagttt aagattttcg acaaaatcga
     5041 agtcaatgga gcaaataccg cgcccttgta tgcctacctg aaatccgtca aaccgcaaga
     5101 taaaggcaat catctgttta aagatttcgt gttgaagctc gcagctttgg gagagaagcg
     5161 cgacgaaggc gacatcaaat ggaactttac caaattcctc gtcaaccgcg acggcgaagt
     5221 ggtcgagcgt tttgcgccta gtgttacgcc cgaagaaatc gaagccgata ttcgggcatt
     5281 gctgtaatct gccgccgtct tgggaaatgc cgtctgaagg cttcagacgg catttgtccg
     5341 tttgcgggtt tattccgaaa tatcggcaac accgctttcg gggaagatag gcaaatccgg
     5401 cggcgggagc agggtatcca aagtcagggc ggacgcatcg agtgtggggt agccttcaaa
     5461 cgcgaccgca taaccgccgt ttccattcat acggattcgg gcgtgcgcgc ctagagggaa
     5521 agtgattttg tcggcaatgt gtccgaacgg gaagcccgtc agcacgggga ttttcgccgt
     5581 gcgcgaaaca tggttggcaa cggcagaaaa atcataagac ggatcataga catctcgaat
     5641 tttttccata cggaaattgc cgaacacgat ggcgcgctgt ttcttcaaaa tacccgaaag
     5701 atacagcgta ttgagcatac gttcgatgcg gtagggctgt tcgccgacat cttcgaggaa
     5761 caaaatgccg ccgtcgatgt cgggcatata aggcgtgccg gcgagcgagg cgaggacgct
     5821 taagttgccg ccccacaata tgccttcggt ttcgacatcg gcgcgttgga tataaggaac
     5881 atcaacggtc aggcggtttt gggttgcacc cttgataaag gcatccatcg taaacgcacc
     5941 gggggcgggt ttgccaaaat cgctataagc catcgggccg gcaaaactca tcatattgcc
     6001 ttttgccaac aatgccagct ggacggcgca tacgtcgctg aatccgaaaa acagcgtgcc
     6061 gtgttcgcgc atccttgcgc cgagcgaagc aaaatcgata tgcggcagaa tccgcgccgc
     6121 accgtaaccg ccgcgcaagc ccatcagcac tttaggcgtg gcgacgcggc cggaagcgac
     6181 ctcttggaaa tcggcgacac gttgtgcgtc cgtaccagca aaccgttgga agcggcggct
     6241 gcccgcctgt tggttggtta cggtaaaacc gacattgtaa aggcgggtta aggctgtgtt
     6301 gacgcggttg gtgtcttcgg caaatcctga cgaagcgaca acgcgcagaa ggttgccgtc
     6361 cgaactttga cggcgtggcg tttggagagg cacggttcgg gctttcacaa cggaatggga
     6421 agagggaagg ggcggaacgg atgtggcgga tgtgccgcaa gcctgaagca gccccgcgcc
     6481 ggcagcggcg gtgcaggttt tcagaaaacg gcggcgggag gtcggttcgg tcataatttc
     6541 cctttcatac ggtttcagac ggcatcggcg ggcttaaagc agctttttaa gctgttccgt
     6601 ccaatcttgc ggcagggtca taccgtgttt ccggacgggg tttgcccaaa tcggcgcggg
     6661 gaaggacgca tcgttttcaa agcgggcgat aatatgccag tgcaggtgcg gcacgacatt
     6721 gcccaagctg gcgaggttga tttttgccgg ccggaacact tggcgcatag cggcttcgac
     6781 tttgtacact atttccatca attcgccgcg ttccgctgcc gaaaggtcgg tcatttcggc
     6841 aatatgctta cgccaaatga cgcggcagaa tgcaggcgaa ccgctgtcgt tatggacggc
     6901 gatgacgcgc agcttggggg tttgcagcaa aatatcttca tcttgtgccg tgcagatggg
     6961 gcagggttgg gcggtcattt ttccttattc ctgaaagtgt ttaaaaaacg gtagggtaat
     7021 ttttaatatt tcaatctgtc tgtccgcatt gccgttaatt gtaccacttc atagggcggt
     7081 gctctatgtg tcgtcctgtg tgttgaaaca tatcggacgc gtttgaacag ttgtgcggca
     7141 aactgtttta gcggcaggca agagatgccg tctgaagccc attgtccgtc attcccacac
     7201 aggcagttgc tacggttgct gaagcccctt agccgtcgcg caggcggttg ctacggttac
     7261 taaaatcccg tcattcccgc gcaggcggga atccagtccg ttcggtttca gttgtttctg
     7321 ataaatcttg cagcgttggg tttttagatt cccgctttcg cgggaatgac ggggcggtcg
     7381 gatgccgcct gaaactccgt cattcttgca acttttcgtc attcccgtga aaacgggaat
     7441 ctagaacttc aaactttcag ataatctttg aatattgccg ttgcccgacg gtttggattc
     7501 ccgcttgcgc gggaatgacg gtggaggacg atgccgtctg aagcccatta gcggtttcag
     7561 acggcatttt tgccggagcg gcagtcagga aaactgcgct gcgccacggt ttgccaaatc
     7621 gtcggcgcgt tcgttttcgg cgtgtcccgc gtgtcctttc acccaagtcc aactgacttg
     7681 atgccgtccg actagagcgt cgagttcttt ccacaagtcg tcgtttttga
//
LOCUS       DAWHRS010000100         7685 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWHRS010000100 DAWHRS010000000
VERSION     DAWHRS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.100"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(213..284)
                     /locus_tag="WIB82_001505"
     tRNA            complement(213..284)
                     /locus_tag="WIB82_001505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Arg,seq:cct)
     gene            complement(304..750)
                     /locus_tag="WIB82_001506"
     CDS             complement(304..750)
                     /locus_tag="WIB82_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ3842790.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(754..1728)
                     /gene="ispB"
                     /locus_tag="WIB82_001507"
     CDS             complement(754..1728)
                     /gene="ispB"
                     /locus_tag="WIB82_001507"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ3842791.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1744..1932)
                     /locus_tag="WIB82_001508"
     CDS             complement(1744..1932)
                     /locus_tag="WIB82_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842792.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            1952..2260
                     /gene="rplU"
                     /locus_tag="WIB82_001509"
     CDS             1952..2260
                     /gene="rplU"
                     /locus_tag="WIB82_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ3842793.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2285..2557
                     /gene="rpmA"
                     /locus_tag="WIB82_001510"
     CDS             2285..2557
                     /gene="rpmA"
                     /locus_tag="WIB82_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ3842794.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            2896..4080
                     /gene="ubiM"
                     /locus_tag="WIB82_001511"
     CDS             2896..4080
                     /gene="ubiM"
                     /locus_tag="WIB82_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ3842795.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRASNNFPPLKKLITKQL
                     TG"
     gene            complement(4269..4424)
                     /gene="rpmG"
                     /locus_tag="WIB82_001512"
     CDS             complement(4269..4424)
                     /gene="rpmG"
                     /locus_tag="WIB82_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ3842796.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(4452..4685)
                     /gene="rpmB"
                     /locus_tag="WIB82_001513"
     CDS             complement(4452..4685)
                     /gene="rpmB"
                     /locus_tag="WIB82_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ3842797.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(4935..6461)
                     /gene="farB"
                     /locus_tag="WIB82_001514"
     CDS             complement(4935..6461)
                     /gene="farB"
                     /locus_tag="WIB82_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ3842798.1"
                     /translation="MDYPPLKGAALAWITLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVIFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSFVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTAGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHSGGGGGH
                     "
     gene            complement(6485..7657)
                     /gene="farA"
                     /locus_tag="WIB82_001515"
     CDS             complement(6485..7657)
                     /gene="farA"
                     /locus_tag="WIB82_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ3842799.1"
                     /translation="MATHADETTIQNRQEANSAKAKRKRCLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         2065 a         2133 c         1783 g         1704 t
ORIGIN      
        1 aatttaatcc actatatcat aaaacaacag atttccaagc cggaacatct tttacggagc
       61 ctgaaaatca tttcatgatt ctaccgtcct aaaggtcggt ttttcaagca ggaagaaaaa
      121 gttttcagat ggcaaaaaag ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc
      181 aacacgggaa acagacaaat gaaatcgtca aacctcgcca acaggaatcg aacctgtatt
      241 ttacgcttag gaggcatacg ttctatccgt tgaactatgg cgagccgaaa tgaaaaggag
      301 attttaaccc tttccgacga caaaagacaa gatgccggcc gcaatcagcg gcccgacagg
      361 gataccgccc ataaatgcca ccccgataac cgtcccgatt aacagccctg taattaaaac
      421 aggctgctgt cccatcaaag gcacgccgcg tcccgccagc caagccacga aaataccgat
      481 aaaaacggcg gatatcattt taaaattcaa aaattcggca acgggaggaa cctgcgcctt
      541 tcctgaaacc aacggactca aaacccctat ggtcaaaaga atgataccga gattcaaccc
      601 gtgcttctcg accaacggga caaactgtac caatgccgtc tgctgcatca gcagcaatat
      661 ggttgccgag atggtaatcg aattgttgtt gctgaccacc cccaacagaa tcagcgtaac
      721 caaaaacagg ggaacaaaac tgaaattcat cggttacgac accctgacca aagattcctt
      781 cgccagctga atcatggcat ccttcacttc gctgtcgggc agggcatcca aggcggcaac
      841 ggcacaatcg actgctttgc gcgcctcgcc tattgaatat gccaacgcat ccgaacggac
      901 gacataatcg tggattttct caaaatagct gcgatctgca ttttccaaag cagtacgcac
      961 atcgttcgca acctgttcgg aaccctgacg catcagataa atcaaaggca aagtcggttt
     1021 tccttccgcc aaatcgtcgc cgacgttttt gccggtttct tcggtttcgc cagaatagtc
     1081 cagcacatcg tcaataattt ggaatgccgt accgacatac ataccgtagt ctttcaacgc
     1141 ccgttcgtgt tcgggggaag ccttgcccaa aattgcgccg acttgagcgg cagcttcaaa
     1201 caattttgcc gttttatatt ggatgacttg gatatattgt tcttcggtaa tgtccgtatt
     1261 gccgatgttc atcagctgca tgacttcgcc ctcggcaata atgttggttg catccgccat
     1321 cacttccaaa acgcgcatac tgcccgaggc aaccatcagt tgaaaggcgc gcgtgtataa
     1381 aaagtcgcca accaacacag ccgccgcatt gccgaacaga ttgtttgccg ttgcccgccc
     1441 acggcgcaaa tcgctttcat cgacgacatc gtcgtgcagg agggtggaag tgtggataaa
     1501 ctcgaccatc gccgccagcg aatacagttt ctcgtcatca taaccgaccg ccttacccgc
     1561 caaaatcgtc ataatcggac gcaggcgttt gccgcccgcg ctgatgatat atgtaccgat
     1621 ttgcgaaatc agtgcgacat cggattgcac cgcacggttg atgacttcat tgactttggc
     1681 aaggtcttca ggcagatgtc gctggaaata gggcagattc tcgagcatgg cagactctca
     1741 ggtttacgtt tccgtcaaac ggcagatacc gcttgaaagc tgattcaaat agcgggcaat
     1801 tatatcagca aagccatcag ggtacatctt ttcagacggc atatgattgt ccgaaccgcg
     1861 caaactttga caaaacaggc aaataacaat acaatgccga gttccgcaca atggtgtgcg
     1921 gatattaacc ataaccctta tggagttgag tatgtacgcg gtcgtaaaaa ccggcggcaa
     1981 acagtataaa gtttccgtcg gcgaaaaatt gaaagtagaa cagataccag ccgaactcga
     2041 cagccaaatc gaactgaccg aagttttgat gattgctgac ggcgaatctg taaaagttgg
     2101 cgcacccttt atcgaaggtg caaaagtaac ggctaaagta gtggcacacg gtcgtggcga
     2161 aaaagtccgc atcttcaaaa tgcgccgccg caaacactac caaaaacgcc aaggccaccg
     2221 ccaaaatttc acccaaatcg aaatcgtggc aatcgcctaa ccttaaaact taggagcatt
     2281 acaaatggca agtaaaaaag caggcggcag cacccgcaac ggtcgcgatt cagaagccaa
     2341 acgcttgggc gttaaagcct acggcaacga gctgattccc gcaggttcca tcatcgtacg
     2401 ccaacgcggt accaaattcc acgcaggcga caacgtaggt atgggcaaag accacacttt
     2461 gttcgccaaa gttgacggtt atgtcgaatt caaaaccaaa ggcgcgctga accgtaaaac
     2521 tgtcagcatc cgtccttaca ccggttctga agaataatcc aaccgatacc tgaagccgca
     2581 tcttttcacg atgcggcttt ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
     2641 cgcagacagt acaaatagta cggcaaggcg aggcaacgcc gtactggttt aaatttaatc
     2701 cactatacat accccacacc cgatttgata cacattaagc tgaaagtaaa aatccgcata
     2761 caccctcccc tgcatatttc ttcaacagtg ggttttgata taatcgccta tctgttacag
     2821 atagttcaaa cggcattcaa acccttacaa ataccgtctg aaatcatcgg catccgtcta
     2881 tgcaaaggat attttatgag tttacacagc gacatcctcg tcgtcggcgc aggcccggcg
     2941 ggtttaagtt ttgcagccga acttgccggc agcggtttga aagttaccct gatcgaacgc
     3001 agcccactga ctgttctgca aaatcctcca tatgacggac gcgaaatcgc cctgacccat
     3061 ttttcccgcg aaatcatgca gcgtttgggt atgtgggaca aaattcccga aaacgaaatc
     3121 taccctttgc gcgatgccaa agtgctgaac ggacgttccg actaccagct ccactttccc
     3181 caaccgactg aagcgcgcgg cgaacctgcc gactgtttgg gctatctgat ttccaaccac
     3241 aatatccgcc gcgccgccta tgaagtcgta tcacaactcg acaatgtcag cattctgacc
     3301 gatacggttg tcaaagaagt caaaacatct gacaacgaag cacaagtcat tttggaaaac
     3361 ggcaaaatac tgactgcacg cctgcttttg gcggcagaca gccgcttctc gcaaacccgt
     3421 cgacaactcg gtatctcctc cgatatgcac gattacagcc gaaccatgtt cgtctgccgt
     3481 atgaaacata ccctttccaa ccaacatacc gcttacgaat gcttccatta cggacgcacc
     3541 atcgcactgc tgccgctgga agagcacctg accaataccg tcattaccgt cgataccgat
     3601 aaaatcaaca gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt aaaagaacag
     3661 ctcaagggca ggctcggcga tatggaattg gtcagcagca tccaccatta cccccttgtc
     3721 ggcatgattg ccaaacgttt ctacggcaaa cgcagcgcgc tgattggcga tgccgccgtc
     3781 ggtatgcacc ccgttacagc acacggtttc aacttggggc tttccagcgc agatattttg
     3841 gcaaaactga tactcgaagc cgaacagcgc ggtcaagaca tcggtgcatc ttcattattg
     3901 gaaaaataca gcagcaaaca tatgctgcac gcccatccgc tttatcacgg aaccaatatg
     3961 atgctgaaac tgtttaccaa tgagaccgca ccggcaaaac tgttgcgcgg cttggtattg
     4021 cgtgccagca acaatttccc accgctgaag aaactgatca ccaaacaact gaccggttga
     4081 aaactgccga aatgccgtct gaaacgtttc agacggcatt tttgtgttat gaaccgtttt
     4141 gcggttttca tatccttggc agacggtttg cagcagattc gcatacggca gatgtttcaa
     4201 gcagaaagga aacacgataa aaataaaaaa gcctccgaac agtcggaggc tttgctttca
     4261 aactgaaatt atttcagttt ggtttctttg tacactacgt gtttgcgggc aaccggatcg
     4321 aattttttga tttccagttt gccgggcata gtgcgtttgt ttttggtagt ggtgtagaag
     4381 tgaccagtac ctgcactgga ttccagtttg attttatcgc gcattgcagt aatccttaat
     4441 aagggtttaa attaagcttc accgcgagca cgcaaatcag ccaatacgac atcaatgcct
     4501 actttgtcga tggtacgcag agcggcgtta gaaacgcgca ggcgaaccca gcggttttca
     4561 ctttctaccc aaaaacgacg tgattgcaag ttaggcaaaa aacgacgttt ggttttgttg
     4621 ttggcgtgtg atacgttgtt gccagacatc gggcgcttac cggtcacttt gcaaactcgt
     4681 gccatggtca gtctccaaac ttctaaaata gtaaaacgtg atttatacca cgaaaaacag
     4741 tgttagttca agtgttttag agaaaaacgg ctgttttacg cgaattatcc tgccgattgt
     4801 tgcattcggg aaacgtttgc cgatacgaaa cagacggaat gcgattatag cgggtttcac
     4861 agatgcctgc attctaaata ctgtccgaac atatttccag tattttcaga cggcatttca
     4921 agttttcaaa tccctcaatg tccaccaccg ccgccgctgt ggaacggcgg ttttgccagc
     4981 catatgacag gaatcatgat gatgaacagc aggctgcccg ccataaagat ttcgttcgag
     5041 ccgataatga aaccctgctg ggtaatggta ttgttgataa tacctgcggt ttgaatgtcg
     5101 gaaacgccgt gctgggacaa atgggcggcc gtttcatgca atgtcgcgga atatggcgtg
     5161 atgtgttcgg caaagcgtgt atggtgcaac gcttcacgcc gttcccacag ggtactaacg
     5221 aatgatacgc cgacaccgcc cataagcacg cgcaagaagt tcgacaagct gccggcggcg
     5281 gcaatctgcc ctcccttcat gtgcgacagt gtgatggtgg tcagcggcag gaagaacatg
     5341 gcaacgcctg ccccctgcca aaactgcggc cagatgacgt tgccgatatc catgtcggca
     5401 taaaaatcgg tgcgccaata gaaagtgaag gcaaaggtca gaaagctggc ggttacgagc
     5461 aggcgcatat cgattttatt gccgaacctg ccgattaacg gagacaggaa gacagggagg
     5521 atgccgacgg gcgcggcggc aagccccgcc cacgtggagg tatagcccag gttggtctgc
     5581 aacactaacg gcagcagggt cagcgtcccc atatacacca taaaacccaa tgacgtggca
     5641 attacgccga cggtaaaatt ccgatcttta aacagcgata aatcgacaat cggatatttt
     5701 tctcccaatt cccaaacaat aaaatacgac aaacacacca gtgcgactac gcccaaggta
     5761 atgatttctc cagaggcgaa ccagtcgagt tccttacccc tgtccagcat catctgtaac
     5821 gcgccgatac cgactaccat caatgtaagc ccgacatagt cggtcggcat tttaacggtt
     5881 tccgtttccc gatatttcaa atgtttccat gtaatccatg ccgatatgat accgataggg
     5941 atattaatga agaaaatcca accccaatgc cagtttccgg aaatccaacc gccgagtatc
     6001 ggcccgagaa caggggcgac aacgacggtc attgcccaca atgccagtgc cagcgtccgt
     6061 tttgcgggcg gataggatgc cattaacagg ctttgcgaca ggggaatcag cggcccggcg
     6121 ataaagccct gcaagatgcg gaaaattacc aaagcctgaa ggttgggcgc gatgccgcac
     6181 agccacgatg tgatgacgaa accgacagcg gcggcggtaa acaatttgac ctcgccgata
     6241 cgttttgcca aaaagcccgt taacggcacg gaaacggcgt ttgccacagc aaaagaagta
     6301 atgacccacg tcccctgagt ggttgccgca ccgaggttgc cggcgataac gggaacggcg
     6361 acattggcga tagtcgtatc taaaacttcc ataaatacgg caagccccaa agacagcgta
     6421 atccatgcca atgccgcacc cttaagcggt ggataatcca ttgtgtcctc cgtttcagac
     6481 ggcatcaggg cgcggattgc ccgaggattt catcgaccgt ccgatcgact tccgaccagt
     6541 cggtactttc catttccggc aatgccgcac ccggcgtttt tgaaacaggc gcgcctgcgg
     6601 cggaagtatc cactttaacc gtcatcgaca aaccgatacg caacggatgc ctgtccacat
     6661 cttcgcggtt cagcacgata cggacgggga cgcgctgcac cactttaatc cagttgcccg
     6721 ttgcgttttg cgccggaatc agcgaaaacg cgctgcccgt acctgccgaa aaacctgcca
     6781 ccctgccgcg ataaacaatt tgtttgccgt acaaatcgga caccagctcg gcaggctgtc
     6841 cgattttcat atgccgcaac tgcgtctctt taaaattagc atccacccac acatccgaca
     6901 gcggcaccac cgccatcagc ggcgcgcctg ccgccacctg ctgcccgacc tgcaccgaac
     6961 gcttcgccac ctgaccgtcc gccggcgcgc ggatttgcgt ccgctgaagg ttcaaccacg
     7021 catctttcaa cctgccgatt gccgtctgaa ccgccggctg ttcgcgcaaa gaaacctgac
     7081 cgcccaaagc cgcacgcgcc gaagattctt ccgccaaagc cgccttaact gccgcctgag
     7141 cctgagacac tgccgcacgg gcatgtgcca gctcttcggc ggacaccgcg cccgattccg
     7201 ccaaagcaga ccggcggcgc aaatcatcct gcgcgcgtgc caaatccgcc cggcgcaagg
     7261 caacctgcgc ccccgcctgc gaagtggcgg cattttgccg gcggttttgc cgcaccgcct
     7321 gaatcagctc gttttttgcc cgctcgtaag ccagcacatc attatcgtcg tccaataccg
     7381 ccagcacgtc gccttttttc acggcatccg tatcgtcgtg caaaaccttc cgcaccgtac
     7441 cgcccttttg cggcgtaacc tgaaccacgc gtccggcaac ataagcgtct tccgtttcct
     7501 cttcgtgctg ccaccataaa aaaaacgccg acccggcggc tgcggcggca agcgcgaaca
     7561 gcagcgtcaa tgccgtcagg cagcgtttgc gtttggcttt tgcgctattg gcttcttgtc
     7621 tgttttgaat tgtcgtttca tctgcatgtg ttgccattat tccaattcca aatacttcta
     7681 atact
//
LOCUS       DAWHRS010000101         7548 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWHRS010000101 DAWHRS010000000
VERSION     DAWHRS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.101"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            15..420
                     /locus_tag="WIB82_001516"
                     /pseudo
     CDS             15..420
                     /locus_tag="WIB82_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WIB82_001517"
     CDS             complement(455..1330)
                     /gene="rsmI"
                     /locus_tag="WIB82_001517"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ3842800.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            1376..1723
                     /locus_tag="WIB82_001518"
     CDS             1376..1723
                     /locus_tag="WIB82_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ3842801.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQLNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="WIB82_001519"
     CDS             1728..2321
                     /locus_tag="WIB82_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ3842802.1"
                     /translation="MTTLQERVATHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="WIB82_001520"
     CDS             2385..2993
                     /locus_tag="WIB82_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ3842803.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGGAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLHQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3651
                     /locus_tag="WIB82_001521"
     CDS             3001..3651
                     /locus_tag="WIB82_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842804.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPRPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKLHKEILDNLF"
     gene            3691..4470
                     /gene="map"
                     /locus_tag="WIB82_001522"
     CDS             3691..4470
                     /gene="map"
                     /locus_tag="WIB82_001522"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ3842805.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4625..4924
                     /locus_tag="WIB82_001523"
     CDS             4625..4924
                     /locus_tag="WIB82_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ3842806.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5162..5536
                     /locus_tag="WIB82_001524"
     CDS             5162..5536
                     /locus_tag="WIB82_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ3842807.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5724..7190)
                     /locus_tag="WIB82_001525"
     CDS             complement(5724..7190)
                     /locus_tag="WIB82_001525"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ3842808.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSTPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1940 a         2274 c         1848 g         1486 t
ORIGIN      
        1 aagaggcgga atgggtgggc agcctgttcg gcctgcacaa atctttcggt ttccttacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa
      121 gcgactgcaa ggctgcggcg gcaggccacg gcattctgta tctgctcatg cttgctgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaagtgat gcaagggttc gccggaaaaa atcgagtgga tggcaaactt gggcaacacg
      301 ttccacggca atttgggctt gctgctgttt gccgccgtcg ccggacacgt cgccatggtc
      361 gtcgcccacc gtgttcaggg tagagatgtt ctgtgccgca tgacgggtcg tgtccgctga
      421 ttccgttcac actatggtgc cggctcgtcc ggcactattt gtttttccaa gacagagcca
      481 gatcgtacaa agctttcttt ccctcgcccg tgattttggc agcaagctcc gccgcctgtt
      541 tggtcggcag ctcggctgtg aggattttca tgatgttttg cgcggactcg gacaagcctt
      601 cgtgtttttc atcctgcgcc ggataaagca ccaacaccat ctcgccgcgc gattggttgc
      661 cgtcggcaga caatgccgtc tgaatttccc caaccgtgcc gcttaagaac gtttcaaacg
      721 ttttcgtaat ttcgcgcgcc agcattaatc ggcgttcggg gaacagttcc gccatatcgg
      781 caagcgtcgc accgatgcgg tgcggcgttt caaacatgac gataggaaac gccgcccgca
      841 cccatttggc aaacagtttc ctgcgttctc ccgatttcgg cggtacaaaa ccgttgaaat
      901 aaaaatcgga tccttccaca ccggccacgc tcaaagccgc catcaccgcg cttgcgccca
      961 cgacgggaac gactttaaac ccggcctcac gcacgcggcg ggcgagtttc gcgcccgggt
     1021 cgcacacggc cggcgtaccc gcatcggaaa cctgtgccac aaccatgccg tctgaaagat
     1081 agccgacaat cttgtccgcc atctgccgtt cgttgtgttc gcgcacactg acgagtttgc
     1141 cctgaatgcc gtacgcgctc aaaagctgtg cggtaacgcg cgtgtcttcg gcacagatga
     1201 tgtccgcctt ttgcaatacc gccaaagcgc gcagggtaat gtccgccaaa ttgccgatgg
     1261 gcgtggcaac cacgtataat gtccctccga cgacgctgtc ggaggctttc tgcaaatgtt
     1321 tctgaaacat aagaatgccg tctgaaaaac aaacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcacg ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgtcgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa ctgaacaggc tgacaaacgt accgtgccgc ctcgatgcgg tacttatcga
     1681 aggaaaccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccacccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaacgacg
     1861 gcaagattct ggcgtgcggc aacggcggtt cggccgccga cgcgcagcac ttcgccgccg
     1921 aaatgaccgg gcgttttgaa aaagaacgta tggaactcgc cgccgtcgcc ctgaccacag
     1981 acacctccgc gctgaccgcc atcggcaatg actacggctt cgaccacgtc ttcagcaaac
     2041 aagtacgcgc cctcggacgc gccggcgacg ttctcgtcgg catttccact tccggcaatt
     2101 ccgccaacgt catcgaagcc gtcaaagccg cacacgaacg cgatatgcac gtcatcgccc
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttttactca
     2221 acgttcccta tcctcgaact gcccgtattc aggaaaacca catcctgctg atacacgcca
     2281 tgtgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagcc cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg cggggcggcg gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgt tcctacctgc
     2581 accaaaacaa ccaaaccaaa ggctacacgc cccaaatctc cgtcgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgagaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtgt acaactacat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgccgtatta
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcgaca aaaccctcca aaccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcgccctg ccgacacagc tcaaaccgac
     3421 agacagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cctacaaacg tcccccgtcc cgaacctcga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacac ccaaagaaaa agaaacgccc aaagaaaacc ataccaaacc ggacaccccg
     3601 aaaaacacgc cgcctaaact ccataaagaa attctcgaca acctcttctg acccggcacg
     3661 gcaggcacac ccgcaatcca aggaagcatt atgaacggca tcatcatcaa aacccccgaa
     3721 gaaatcgaaa aaatgcgcga gctgggcaaa ctcgtcgccg aagccctcga ctacatcgga
     3781 caattcgtca aacccggcgt aaccaccgac gaaatcgaca aactcgttta cgactaccac
     3841 gtcaacgtcc aaggcggtta tcccgccccc ctgcactacg gcaacccgcc ctaccccaaa
     3901 tcctgctgca cctccgtcaa ccacgtcatc tgccacggca ttcccgacga caagccgctc
     3961 aaagaaggtg acattatcaa catcgacctc accatcaaaa aagacggctt ccacggcgac
     4021 tccagccgta tgtttaccgt cggcaaagtc tcccccatcg cccaacggct gatcgacgta
     4081 acccacgcct ccatgatggc gggcatagaa gccgtcaaac ccggcgcgac actgggcgac
     4141 gtaggctacg cctgccaaca ggttgccgaa aacgccggct attccgtcgt acaggaattc
     4201 tgcggacacg gcatcgggcg cggtttccac gaagccccgc aagtgttgca ctacggaaaa
     4261 aaagggcagg gccccgttct aaaaccgggt atgattttta ccgtcgaacc gatgatcaac
     4321 caaggcaaac gccacctgcg tatcctcaac gacggctgga cggtggttac caaagaccgc
     4381 tccctctccg cccaatggga acacgaagtt ttggtgaccg aaaccggcta cgaaatcctc
     4441 accgtcagcc ccgcctccgg caaaccctga aaccggacgt atccgcccca taaaaacaaa
     4501 caatgccgtc tgaaagaaac ggcagatatg atatataata taaaaacagg cttgacccgg
     4561 cacattacga aaacaaagca aatcggaatt tgccccgcaa ccagacaaac ttaaaggaag
     4621 ttttatgaaa atatttgaaa atatagaaga tgttaaagcc atccgtaaaa agaccgggct
     4681 gaaccagata gacttctggg gcaaggtcgg cgttacccag tccggaggat cgcgctacga
     4741 aaccggccgc aaaatgccca aacccgtacg cgaactgctc cgcctcgtcc atatcgaatg
     4801 catcgatttg gcgaaagtca acaaaaaaga tatggaaatc gccgccctgt tgaaaaaaca
     4861 ccatcccgac ctgtatgccg aattgtccaa acagaccaag tccgaaagaa aaaaacaaag
     4921 ttaaaccgca acctccggat gcccgacagt ttttcatttc cgaaaaacgc aaacaatgcc
     4981 gtctgaaaca ccggacaggc cgccgtatcc cgcctgccgc ccctgcctca aaccgccgaa
     5041 ccgcccgaac ccgccttttt acaaacttta tccaatttcc tgtttatttc aggatacgcc
     5101 gacattagaa tgtcaaacag ctcgaaacgg gcaaactcca catccatcca aaggaataaa
     5161 aatgaaactt ctgaccaccg caatcctgtc ttccgcaatc gcgctcagca gtatggctgc
     5221 tgctgccggc acgaacaacc ccaccgttgc caaaaaaacc gtcagctacg tctgccagca
     5281 aggtaaaaaa gtcaaagtaa cctacggctt caacaaacag ggtctgacca catacgcttc
     5341 cgccgtcatc aacggcaaac gtgtgcaaat gcctgtcaat ttggataaat ccgacaatgt
     5401 ggaaacattc tacggcaaag aaggcggtta tgttttgggt accggcgtga tggatggcaa
     5461 atcttaccgc aaacagccca ttatgattac cgcacctgac aaccaaatcg tcttcaaaga
     5521 ctgttcccca cgttaatcag gcaacaaaaa acagcgtttt cagaaatgaa aacgctgttt
     5581 ttttgaccgt tccattattc gcaaaaggga aaaaacgatt acctgccccg tgtatcaaaa
     5641 cctgccctgc cggatgaagg gcataaccgg cagggacggc atcaacacca tatgggggta
     5701 cggcttttct tgaaagattc ggcttaaata tccaacactt tcgcggtata ggcgataatt
     5761 tcatccgccc tttcggggtt ttcgttcaac ttgatgccgt aacccggtac cagctctttc
     5821 agacggcctt cccaagacgg ggcgcgctcg gggaagcatt ggtgcatcaa ccggatcatc
     5881 agcggcacag cagtcgatgc gcccggcgac gcgcccagca atgcggcgag tgagccgtcg
     5941 gcgtgggcga caatctccgt accaaactgg agcacgccgc ctttttcgga gtctttttta
     6001 atgatttgga cgcgttgccc tgcggtgatg agttcccagt cgtcggggtt tgcctcgggg
     6061 tagtattcca gcagggaggc gaagcgttct tctttggttt tacgcaattc gcccagcagg
     6121 tatttggtca gcggcatatt cgcccagccg gcgcacagca taggatagag gttgtccata
     6181 tggatggaca gcggcaaatc cataagcgag ccttgcttga ggaagttgga acggaagcct
     6241 gcgtaagggc cgaacataag gtggcgtttg ccgtccacgt tgcgtgtgtc gaggtgcggg
     6301 acggacatcg gcggcgcgcc gacggaagcc tgcccgtaca ctttggcgtt gtgttgttcg
     6361 gcggtttcag gggtgctgtt gcggaagaac aggccggaca cggggaagcc gccgtagcct
     6421 ttgccttcgg ggatgccgga tttttgcagc agggtcagcg cgccgccgcc cgcgccgagg
     6481 aagaggaagc gggtacggag ggtgagctgc ccgtcggggt tgcgggtatc ggcggttttg
     6541 agcacccacg cgccgtcgga ttcgcgtttg atgtcttcga cgtggcggtt gaactcggtt
     6601 tttacgccct tgccctgcaa atatttcacc atttggcgcg tcagccgtcc gaaatcgaca
     6661 tccgtgcctt cggcggagta gttggcggcg acgggttggt tttcgtcccg gccgcgcatc
     6721 atcagcggag cccaatcgga aattttgttc cgatcggtgg aaaattccat attttcaaaa
     6781 agtttttggg ttttaaacgc gtcataacgt ttttgaagat aagaacaatg gtcttcattc
     6841 atcaccaaag acatatgcgg cacggcattg atgaaggaat tgtcttccaa cttgccttcc
     6901 gcgaccaacg tcgcccaaaa ctggcggctg acatgaaact gttcggcaat attgagggcg
     6961 cgcgccggat cgataatccc atttgcaccc aacggcgcat agttcaattc gcacagcgcg
     7021 gaatgccccg tgccggcgtt gttccacgcg tttgacgatt ccaacgccac atcttccaag
     7081 cgttcaatca gggtgatttc ccaagacggt tcgagttctt tgagcaaaac gcccaaagtc
     7141 gcgctcataa tgccgccgcc caccaagaca acgtctgtcg cttcagccat ggtttactcc
     7201 taaaaaacag gcatcttctg cccttatggt tatttgccgt actacaaacg cctgaatcgc
     7261 aaaagcaggg aaaaccggca atggtgtgtg tccgagtatg ctgtttcggg gttggaatgc
     7321 gttgcaagta tggcttccga caccgcttca ggggcttgta atatgttatc gtgaatgtag
     7381 tggattttac tgggaaatgc aaagtttttc tgtcgcccgc caagcccggg aactgcgaaa
     7441 tgaaaaataa aaatagttat ttatctatat atatcaaatt tttaatagat aaaaaaccaa
     7501 aattgtttat atattaattt ttaaaagatt gtcagcatat tgcgttaa
//
LOCUS       DAWHRS010000102         7499 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWHRS010000102 DAWHRS010000000
VERSION     DAWHRS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.102"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            385..1830
                     /gene="zwf"
                     /locus_tag="WIB82_001526"
     CDS             385..1830
                     /gene="zwf"
                     /locus_tag="WIB82_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ3842809.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2110..2805
                     /gene="pgl"
                     /locus_tag="WIB82_001527"
     CDS             2110..2805
                     /gene="pgl"
                     /locus_tag="WIB82_001527"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ3842810.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            2786..3772
                     /locus_tag="WIB82_001528"
     CDS             2786..3772
                     /locus_tag="WIB82_001528"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ3842811.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGTSPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            3822..4670
                     /gene="hexR"
                     /locus_tag="WIB82_001529"
     CDS             3822..4670
                     /gene="hexR"
                     /locus_tag="WIB82_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ3842812.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            4791..6437
                     /gene="pgi"
                     /locus_tag="WIB82_001530"
     CDS             4791..6437
                     /gene="pgi"
                     /locus_tag="WIB82_001530"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ3842813.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEV
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            6451..7002
                     /locus_tag="WIB82_001531"
     CDS             6451..7002
                     /locus_tag="WIB82_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ3842814.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSELACKTVETEARLEELY
                     NRKKALIDEMVREADKKELPKRL"
     gene            7047..7187
                     /locus_tag="WIB82_001532"
     CDS             7047..7187
                     /locus_tag="WIB82_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842815.1"
                     /translation="MPEYRNWQNGLNAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
BASE COUNT         1935 a         1969 c         1971 g         1624 t
ORIGIN      
        1 tatctgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actatatttt
       61 aaaaggtatg ccgtctgaaa gtgggaagcg ggtgttatta tagtacaaaa tttgtaattt
      121 tgttacaaaa aaatcaggca ttgaaaagaa atttgattta aatcgcagaa tctttcgatt
      181 gttgtaacgg aataggggaa acctgcccga tttttggact aggtcaaata aattgtaaaa
      241 atgggaatag acaaaaagtg tagcggtgtg tagtattact actcattatg ccgcctgcct
      301 ttgtttccga tattgcgatg cagcgtgatg ggggaaacag gtttcagacg gcatcgaaaa
      361 attctttcct gacgagataa tacgatgagt acacaaacaa attttgattt ggtgttgttc
      421 ggtgcgaccg gcgatttggc gatgcgcaag ctgttgccct gtctgtacca ggcgcatgtt
      481 gccggtctgc ttcatcccga agggcgtatt ttgggggtca gccgcagcga gttggatacc
      541 gaaggttttc tggcgaaggt ggaaaccagt tccaaaatcc atgtgaaaga aaatttttcc
      601 gatgaggctt gggcatcgtt tgtcgaacgt ttcgcatatc tcaaagtcga tgttacgcag
      661 ccggacgatt ttgccgcttt gggtgatttg gtcaaagcgc gcaaagaaac cgacaatgtc
      721 gtcatctatc tttccaccgc gccgaaattc ttcgcgcaag cctgtgaaaa ccttgctgcg
      781 atcggtttga atgccgacaa tgtgcgcgta gttttggaaa aaccgttggg tacggatttg
      841 gcatcttccc aacaaatcaa taccgatgtt gcgcgttatt tcaaagaagg gcaaatctac
      901 cgtatcgacc attatttagg taaagaaagc ctgcaaaacc tgctggcctt gcgttttgcc
      961 aatgtgatgt tcgagccgct ttggaacaat aaatacatcg aaagcgtgca gctgaccatc
     1021 gccgagcagt tgggcgtgga agaacgcggc gagttttacg acattaccgg cgcgttgcgc
     1081 gatatggtgc aaaaccactt gatgcaaatg ctgtgcatga ctgcgatgga agcccccgcc
     1141 agcttggatg ccgacgcggt gcgcgatgaa aaagtcaaag tcatcaagtc attgaagccg
     1201 ctgaccgtcg aatctgtcaa tgaaaatgtc gtgcgcggac aatataccgc cgccaaaggc
     1261 atgaacggct atcttgaaga aatcaacgtt ccgcaagaca gctttaccga aacctacgtc
     1321 gccattaaag ccgaaatcga aaacgaacgc tggaagggcg ttcccttcta cctgcgtacc
     1381 ggcaagcgca tggcgggcaa agtggcggaa atcgttttga acttcaaaga tttgaacagc
     1441 catatttttg aaggcagtcg caccgcgccc aaccggctcg ttatcgagtt gcaaccatat
     1501 gaatccgtgc gcctctatac gcagatgaaa accccggggg caggaaataa ggtcgaaacc
     1561 gtgccgctgg caaccgattt gggcaaagca ttggaaagcc gccgcgcgga agcttacgag
     1621 cgcctgctgc tggatgtgat taacggcaaa ctcgctttgt ttaaccgccg cgacgaactt
     1681 gaagccgcgt gggaatatgt gatgccgatt ttggaaaact ggacaaataa caccacgccg
     1741 ccgcacggct atggcgcaca ctcgtggggg cctgaagccg cgcgcgaact attggcgcgc
     1801 gacggacaca agtggcacga agagcagtaa tacaataatg cgttcagacg gcatggggtt
     1861 tgaaatgccg tctgaacgta agtaaagcag tatagtggat taacaaaaat caggacaagg
     1921 cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgctg tactggttta
     1981 aatttaatcc actataaaaa gcagtcccga tatttggtat caaaagcata aacaactgtt
     2041 cgaccggcat attgctcaac ccgtttccat aacggaatat gccgtctgaa ataaaaaaag
     2101 gacacaaata tgtttgtttg gcacgaatac gaaaatgcgg cagaagcggc gcagtctttg
     2161 gctgacgcag tggcggatgc tttgcagggc gcactggacg agaagggcgg tgcggtgttg
     2221 gcagtttccg gcggacgttc gccgattgca tttttcaacg ccctgtcgca aaaagatttg
     2281 gattggaaaa acgtcggcat caccttggca gatgaacgca tcgtgccgac cgtccacgcc
     2341 gacagcaata ccggtttggt gcgcgaatac ctgttgaaga acaaagcgga agcggcaatg
     2401 tggattccta tggtggaaga cggaaaaact gaaaccgaat tacatcccga tgctgttgtc
     2461 gattatgcac tgaaacatta caaacagccc gatgttttgg ttttgggtat gggaaacgac
     2521 gggcatacgg cttcgatttt cccgaaagct ccacagtttc agacggcaat cgacggttcg
     2581 gcaggtgtcg cgctggtgca taccacgccc gttaccgcgc cgcacgagcg cgtcagtatg
     2641 accttggatg cgattgccca tacggggcat gtgtttttgg cgatacgggg cgaagagaaa
     2701 aaagccgtgt tcgaccaagc cgcacaaggc gaaaaccgcg aatatccgat caacctcgtt
     2761 ttgaaccatc aaggagtgaa ctgccatgtc ttctacgccg aataaacaag ccggatatcc
     2821 ccgactggtc gccgatatcg gcgggacgaa tgcacgcttt gcgctggaaa ccgcgccgcg
     2881 cgtcattgaa aaagccgccg tgcttccgtg taaagactac gatacggtta ccgatgcggt
     2941 gcgtgcctat ctgaatcaaa gcggtgcaac agccgtacgg cacgcggcat ttgccatcgc
     3001 caacccgatt ttgggcgact gggtgcagat gaccaaccac cattgggcgt tttccatcga
     3061 aaccacccgt cagactttgg ggctggacac cctcatcctt ttgaacgact ttaccgcgca
     3121 ggcattggcg gtaacgcaga cttcaagcaa agacctgatg caggtaggcg ggcaaaagcc
     3181 tgtcgaattt gcccccaaag ccgttatcgg ccccggtacc ggcctgggcg tgagcggatt
     3241 ggtgcacagc cacgcaggct gggtggcttt ggcgggcgag ggcgggcata ccagtttccc
     3301 gccgtttgac gatatggaag tgctgatttg gcagtacgcc aaaaacaaat acggccatgt
     3361 ttccgccgaa cgctttttga gcggcgcggg cttgagcttg gtttacgagg ctttggctgc
     3421 aaaacagaaa gccaaacccg ccaaactgat gccgtctgaa atcacggaaa aggctttgag
     3481 cggcacgtcg cctttgtgcc gtcagacttt ggacatcttc tgcgccatgc tcggcacggt
     3541 tgcttccaac ctcgccctga cgctgggcgc gcgcggcggc gtgtacctgt gcggcggcat
     3601 tattccccgc gtgttggaat acttcaaaac ttccccgttc cgcagccgtt tcgagaacaa
     3661 gggcaggttt gaagcatatc ttgccgcgat tcccgtgtat gtcgtcttga gcgagtttcc
     3721 cggaatttcc ggtgcggctg cggctcttga caaccatttg agaaacgttt aaccacagcg
     3781 gctccttgca gcggggctgc attatcgaag ggcatatcat tatgttaagc aaaatcagcg
     3841 aatcactggc aaacctttcc ggtgcggaac gcaaagtcgc cgaatgtgca ttggcggaac
     3901 ccaaatggtt cgtccatgcc gccgttgccg aaattgccga acgtgcgtcc gtcagccagc
     3961 cgaccgtcat ccgattctgc cgcagcttgg gttataaagg gctgcccgag ttcaagctcg
     4021 ccttgtccgc cagcatcggt catgagggta tgccctatgt ccacgaagaa ctcaacgccg
     4081 acgacgatat ggcaagcgtg gtcgagaaag tgttgggcaa tgccgccgcc tcgctgttgg
     4141 gcgaacgccg cttcctgaaa gagtcggagc tggaaaacgc cattgccacg ctgatgcacg
     4201 cccgtcgcgt cgagttttac ggtgtcggca attccggcat tgtggcacag gacgcgcagc
     4261 ataaattttt ccgtttcggc atgtccaccg tcgcctatgt cgatacgcac acgcagctga
     4321 tggcggcatc tgttttgagc gatcaggatg ttttggttgc catttccaac acgggttcgt
     4381 ctatcgaact tttggatgcg gtcagcatcg ccaaagaaaa cggcgcgtct gtcatcgcac
     4441 tgacccgcaa cgattcgcct ctggcgcaac ttgccgactg cgtgttgagc gttgccacac
     4501 aggaaaatgc cgaactctac acgcccatgg tttcccgcct cttgcagctt gccgtcatcg
     4561 acattctcgc catcggactt gccctgcgct tgggcgatgc tgccagcctg caattgcaga
     4621 aaagcaaaaa aagcatacac aacaagcaca tcgattacga caaagattga ccttcagacg
     4681 gcatcccaca aatgccgtct gaaatgccga acaacggtcg tcggcggctt gcggcagttt
     4741 ccggcagcct tttcatccca caacaaaaaa cctcattcag gagcatatag atgaaacacc
     4801 ttcacgactt acccgcatgg tcgaaattgt ggaatcactt tgacgacagc aaaacattgc
     4861 atatgcgcga aatgttcgag caagacccgc agcgtgcgga acgctactgg ctgcaagtcg
     4921 gcggactgac gctggactac tccaaaaacc gcatcaacga cgaaaccatg tcgcttttgt
     4981 tcgagcttgc ccgagaagca ggcgtgccgg agcggatgcg gcagatgttc cacggcgaaa
     5041 aaatcaatac caccgaaaac cgcgccgtcc tgcatgtcgc ccttcgcaac cgcaccaatt
     5101 cgccgattgt ggttgacggt gaagatgtga tgcccaaagt caaccgcgtt ttgcaacgta
     5161 tgggcgaatt tgcacacgaa gtccgcagcg gaagctggct gggctatacc aaccaagtca
     5221 ttaccgacgt tgtcaacatc ggcatcggcg gatcggattt gggtccgctg atgatgtgta
     5281 ccgcgctcaa acctttcggt catccgcgcc tcaatatgca cttcgtctcc aacgtggacg
     5341 gctcgcaact gcgcgacgta ttgtccaaag tccaccccga aaccacgttg ttcatcatcg
     5401 cctccaaaac atttaccacg caggaaacgc tgaccaacgc gctgaccgcg cgcgaatggt
     5461 ttttgaatca tgcgggcgac gaagaagccg ttgccaaaca cttcgccgcc gtttccacca
     5521 atcaaaaagc cgtcgccgaa ttcggcatcg acaccgccaa tatgtttgaa ttttgggatt
     5581 gggtcggcgg tcggtacagc ctgtggtccg ccatcggatt gccgattatg ctgtatctcg
     5641 gcgaagaaaa cttcattgaa atgctcaacg gcgcgcacct gatggaccaa cacttcatca
     5701 acacaccgct cgagcgcaac ctgcccgtca ttctcgccct catcggcatc tggtatatca
     5761 actactacgg cggcggcagc cacgtcatcg cgccttacga ccaacatttg caccgcctgc
     5821 ccaaattcat ccagcagctc gatatggaaa gtaacggcaa acaggttacg ttggacggca
     5881 aagcagtcgg acacgaaacc tcgccgatta tctggggcga aacgggcatt aacggccagc
     5941 acgccttttt ccaactgctg caccaaggca cgcacattac ccccatcgac ctgattgcct
     6001 cgcttgaaaa acgcagcaac ctgcccggac accacgaaat cctgcttgcc aacgtcttcg
     6061 cccaagcaga agcctttatg cgcggcaaaa cccccgacga agtccgcgcc gaactcaaag
     6121 cgcagggtat ggatgaggtg cgcatcgaag agctggtccc gcacaaaacc ttctccggca
     6181 accgcccgac caacctcatt ctcatggaca aggtcaaccc gcgcaatatg ggcagcctga
     6241 ttgccatgta cgaacacaaa accttcgtac aaggcatcat ttggggcatc aacagcttcg
     6301 accagtgggg cgtggaactc ggcaaacaac tggctaaaac cattttgggc gaactgaccg
     6361 gcgaaaccgg gccgcaaaag cacgacagtt cgaccgaacg cctgatcaac ctctacctgc
     6421 agaccaaccg caaataaaac ctgcggaaaa atgccgtctg aacgccgacc gtttcaaacg
     6481 gcatttttat cgaacaggaa aaccgtcggt aaacttgcag agcgtgtgca atcccgatat
     6541 gatggtttgc ataaatttaa acatatatgt tccgcagcta tggcactgat taaagagccg
     6601 ttggacaaag cgaaacaaag gaacgaagaa cttgaagcgg cagaagaagc ggcggcgcag
     6661 gaggcattgg gtcgggagca ggaagccgcc cgcgtatccg aatgggaaga acgctacaag
     6721 ttgtcgcgca gcgagttcga gcagttctgg aaaggattgc cgcaaactgt acagaataaa
     6781 ctgcaagctt cgcagaaaac atggaaaagc ggtatggaca aaatctgtgc caacaatgcg
     6841 aaagccgaag gtgaaacgcc aaacggcata aaagtcagcg aactggcatg caaaacggtg
     6901 gaaaccgaag cacgcttgga agagctgtac aaccgtaaaa aagcccttat cgacgaaatg
     6961 gtcagggaag cggacaagaa agaactgcca aagcggctct gaacagcgcg gttcaggcac
     7021 tgcccgccga tattgccgaa accgtcatgc ccgagtaccg caactggcaa aacggtctga
     7081 atgccaaatg cgctgacggc gacgaatacg gcgtggcaca atccgactgc cgtaccagag
     7141 aaatcaatgc gaaaaccaaa gaaatccaag gttatctgat tgactgaaac ttggatgcgg
     7201 gaatgtcggc ggcttttgcg tttttgtcgt ttttatagtg aattaaattt aaaccgatac
     7261 atcgttggct cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga
     7321 atcggtccgt actatttgta ctgtctgcgg cttcgccgcc ttgtcctgat ttttgttaat
     7381 ccactatatc tttttccact tatctgcgct ttcactattt gccctttcag gctgcgggca
     7441 tagggacgga acaggtagcg gtcaaatcct gtttcatcca aataaacacg ttggtagtc
//
LOCUS       DAWHRS010000103         7388 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWHRS010000103 DAWHRS010000000
VERSION     DAWHRS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.103"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(68..427)
                     /gene="rnpB"
                     /locus_tag="WIB82_001533"
     ncRNA           complement(68..427)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WIB82_001533"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(506..1609)
                     /gene="prfB"
                     /locus_tag="WIB82_001534"
     CDS             complement(506..1609)
                     /gene="prfB"
                     /locus_tag="WIB82_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ3842816.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1712..2605)
                     /locus_tag="WIB82_001535"
     CDS             complement(1712..2605)
                     /locus_tag="WIB82_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ3842817.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2947..3396
                     /locus_tag="WIB82_001536"
     CDS             2947..3396
                     /locus_tag="WIB82_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ3842818.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3470..3778
                     /locus_tag="WIB82_001537"
     CDS             3470..3778
                     /locus_tag="WIB82_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842819.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAADKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3895..4737
                     /locus_tag="WIB82_001538"
     CDS             3895..4737
                     /locus_tag="WIB82_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ3842820.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFIEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            4727..5473
                     /locus_tag="WIB82_001539"
     CDS             4727..5473
                     /locus_tag="WIB82_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ3842821.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5495..6082
                     /locus_tag="WIB82_001540"
     CDS             5495..6082
                     /locus_tag="WIB82_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ3842822.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6088..6267
                     /locus_tag="WIB82_001541"
     CDS             6088..6267
                     /locus_tag="WIB82_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ3842823.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6499..6939
                     /locus_tag="WIB82_001542"
     CDS             6499..6939
                     /locus_tag="WIB82_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842824.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1908 a         1960 c         1672 g         1848 t
ORIGIN      
        1 cacaacctaa ataaaaatgc cgtctgaacc atatttcagg tttcagacga catttgcgtg
       61 tcggatgcac accggacagg cggtaagccg ggttctgtct cggacagtca ttcctctagg
      121 cataccgtta ccggtatgct caagcaacct acccgaacgc tcggcgggca gcgtcattgc
      181 gttctgtttg gtcttgctcc gaatggggtt tggcctgccg catattgtta ccaaatgcgc
      241 ggtgcgccct taccgcacct tttcaccctt gcctgtgctg ccaaagcagc catcggcggt
      301 tttgctttct gttccacttt ccgtcgcgtt accgcgcccg gccgttaacc ggcattctac
      361 cctgcggagc ccggactttc ctccccgtat gccttacgcg atacgcggcg actgtctgct
      421 cgtcccgtgt gcggcgcgga ttataacacg aaacgcaaaa atgccgtctg aaacggtaca
      481 ggtttcagac ggcatacagc ctaaactaca cgccctgttt caggctggct tcgataaagc
      541 cgtccaaatc gccatccaat acggctttgg tgttgccgac ttcgtagcct gtacgcaagt
      601 ctttgatacg tgaggaatcc aaaacatacg aacggatttg gctaccccaa cctacatcgg
      661 atttaccttc ttccaacgcc tgtttctctt cattgcgttt gcgcatttcc aattcataca
      721 gtttggactt caacatttcc atcgcggcgg ctttgttggc gtgttgcgaa cggtcgtttt
      781 gacattgcac cacaatcccc gtcggctcgt gggtaatacg cacggcggag tcggttttgt
      841 tgatgtgctg accgcccgcg cccgatgcgc gataggtgtc gatacgcaaa tcggcggggt
      901 tgatttcgat ttcgatggaa tcatcgattt cagggtaaac gaacacggag gcaaacgaag
      961 tatggcgttt gttattcgag tcaaacggtg aatagcgcac caagcggtga acgccagttt
     1021 cggtacgcag caaaccatag gcgtattcgc cttccactcg gatagtcgcg cggttgatgc
     1081 ccgcgatttc gccgtcgtct tcttcgagga tttcgatttt gaagcctttg cgctcggcgt
     1141 agcggctgta catgcggaac agcatacccg cccagtcttc cgcttccgta ccgcccgcgc
     1201 ctgcggtgat gtcgataaag cagttgttcg ggtcggcggg ctggttgaac atccgtttga
     1261 actccaaatc cgccatctgt ttttccagcc ccgccacatc ttcctgcacg gcggcaaaac
     1321 cttcttcgtc gttttcttcg acggtcattt caatcagcat gcggttgtct tcgatgcccg
     1381 aagcgatgtt gtcgagcgtc aacacgatgc cttcgaggat tttgcgctct ttgccgattt
     1441 cttgggcgcg tttcgggtcg ttccaaagtt cggggtcttc ggaaagaccg acaacttctt
     1501 ccaatcggtc tttcttaccc tgataatcca tataaacgcg gatgtcttcg ctgcgctttt
     1561 ccaaatcatt cagggtattg ttgagctggt tgattacttc ggcttccatg attcttttgt
     1621 tctttcaaaa ttttaggggc gtattgtacg ggattcgggt atttttttct atggataaag
     1681 ccttctagaa acacgttcgg acggcatagc gtcaataacg gtatgccgcc agtttgcgtt
     1741 tgatttcagg caatgcggca cgtgctgcct cctcacccaa ctggatggca cgttttttct
     1801 gatcgaatcc gccgactgca cccaaatcca aaacctgcgg tttgataacc acatccgcct
     1861 gccccaactc attttgcaac gcggaaacgc tcattacgtt cagcgtctga tcgagataag
     1921 agaagaagcc ttggctgatg tttttgcccg gacgggcgga aatatcgacg gcaatcacga
     1981 aattcgcccc ctgccgccgg gcggcactga cgggcacggg ctgcgacaga ccgccgtcaa
     2041 catatgtatg cctgccgatg ataacgggtt ggaacacatt gggaatggcg gcggaagcgc
     2101 gcacagcctg cccggcattc ccttgattga aagcgacggc cttgccggtt tcaaaatcag
     2161 tagcaacagc ggcaaatttg atgggaaact gctgaatctg cctgccgccg acttttcggt
     2221 tgatgtaatt ttgcagcttt tcgcctttga taaaaccact ggtggacaag gttaaatcga
     2281 ctaaatcggt tttgcctaaa atttcggctt ccaattcgag gcggtcgggc gacatacccg
     2341 atgcaaaaag gctgccgaca atcgaacctg ccgatgtgcc ggtaaccacc ttcacaggaa
     2401 taccgttttc tttcaaaacc ttaataatac ctacatgggc aaatccttta gatgcgccgc
     2461 caccgagtgc caaaccgacc actgcggcgg gtttggcggt ttgcaccggc ttgcgggcgg
     2521 cattgtttcc cgccgtgccg caggcggcaa gcaacgcggc ggcggcgatt gccaaaagcg
     2581 gtctgatttt tgaaaacgtt accatatttt ccattccttt atatatcgca ccccgtcaaa
     2641 aagagggatt gcttttctta acaaccccct ttgacagcca agcaaatggg ggctttgtta
     2701 agtcatcatc aaaattaata tttctttttt tcctttacgg aaattatatt tgaaggcata
     2761 ctatccaagg cgggaattat ctcacaacac cgccgttatc caaatatccc gcctttttcc
     2821 tttcctttcc atcaaaatac tttcttttta tattcattaa cttgttaaat cattggctgc
     2881 cgggtgtcag tttttccgac aaaatccgtc taatggggta tcaacagaac caaaacagga
     2941 acacttatga aaatcggaac aacttggcag acggcatccg ctatgctggt tttgcgtctg
     3001 tttgccgcat atgaattttt ggaatcgggt ttgcaaaaat ggaacgggga gaattggttt
     3061 tctgaaatca acgatcaatt cccattcccg ttcaacttgc tgccggacgc gttaaactgg
     3121 aatctcgcta tgtatgcgga gcttttactg cccgtactgt tgcttttggg tttggcaacg
     3181 cgtctgtcgg cattagggct gatggtcgtt accgccgtcg cttgggctgc ggttcacgcc
     3241 ggttcgggtt acaatgtctg cgacaacggt tataaaatgg ctttaattta tatcgtggta
     3301 ttaatcccgc tgcttttcca gggtgcgggc ggatggtcgc tggatacgct gctgaaaaaa
     3361 cggttttgcc cccaatgccg tctgaagcaa gattgattca gtcgtggaat ctgactttaa
     3421 acattccaac cttatctcgt taacttgata ttctgaaaag gaaatagaaa tgaacaaaaa
     3481 cattgctgcc gcactcgccg gtgctttatc cctgtctttg gccgccggtg cagttgccgc
     3541 cgacaaaccg gcaagcaacg caacaggcgt tcataaatcc gcccatggct cttgcggcgc
     3601 gtccaaatct gccgaaggtt cgtgcggcgc ggctggttct aaagcaggcg aaggcaaatg
     3661 cggcgagggc aaatgcggtg cgaccgtaaa aaaaacccac aaacacacca aagcatctaa
     3721 agccaaggcc aaatctgccg aaggcaaatg cggcgaaggc aaatgcggtt ctaaataatc
     3781 ccaccccttc aaaccaagcc gcgtttttca gtaaaatgcg gcttttttta acggcaaaca
     3841 aagatttttt aacaagcaca tcattctttt gtgccatccg aaccgggtaa aaatatgatt
     3901 caacacgcag gcttgggcta ccgccgcgac ttggcggaag actttctctc gctttccgaa
     3961 aacagcccga tatgctttat cgaagccgca ccggaaaact ggctgaaaat gggcggctgg
     4021 gcgcgcaaac agtttgaccg tgtggcggaa cggctgccgc tggcgttgca cggattatct
     4081 atgtcgctgg gcgggcaagc accgctggat actgatttga tagacggcat caaagaaatg
     4141 atgcgccgtt acgattgcac gtttttctcc gaccatttga gctactgcca cgacggcggt
     4201 catctttacg atttgttgcc gctgcccttt atcgaggaaa tggtgcatca tacggcgcgg
     4261 cgtatccgcg aagtgcaaga ccgtttgggc tgccgcatcg ccgtggaaaa cacgtcctac
     4321 tatctgcatt ccccgcttgc cgagatgaac gaggtcgagt tcctcaacgc ggttgcacgt
     4381 gaggccgatt gcggcattca tctggacgtg aacaatatct acgtcaacgc cgtcaatcac
     4441 ggcctgttgt cgccggaggc ttttttgaaa aacgttgacg cagatcgcgt gtgctatatc
     4501 catattgccg gacatgacgt ggaaacgccg gaattgttga ttgatacaca tggcgcggca
     4561 gttttgccga ctgtttggga cttgctcgaa cttgcctatg ccaaactgcc gacgattccg
     4621 cccaccctgt tggaacgcga ttttaatttc ccgccttttg ccgaactcga agccgaagtc
     4681 gccaaaatcg ccgattatca aacgcgtgcc ggaaaggaat gccgccgtgc agcctgaaac
     4741 ctccgcccaa taccagcacc gtttcgccca agccatacgc gggggcgaag ccgcagacgg
     4801 tctgccgcaa gaccgactga acgtctatat ccgcctgata cgcaacaata tccacagctt
     4861 tatcgaccgc tgctataccg aaacgcggca atactttgac agcgaagaat ggagccgtct
     4921 gaaagaaggt ttcgtccgcg acgcgcacgc ccaaacgccc tattttcaag aaatccccgg
     4981 cgagttccta caatattgcc aaagcctgcc gctttcagac ggcattttgg cactgatgga
     5041 ttttgaatat acccaattgc tggcagaagt tgctcaaatt ccggatattc ccgacattca
     5101 ttattcaaat gacagcaaat acacaccctc ccctgcggcc tttatccggc aatatcgata
     5161 tgatgttacc gatgatttgc atgaagcgga aacagccttg ttaatatggc gaaacgccga
     5221 agatgatgtg atgtaccaaa cattggacgg cttcgatatg atgctgctag aaataatggg
     5281 ttcctccgcg ctttcgtttg acaccctcgc ccaaaccctt gtcgaattta tgcctgagga
     5341 cgataattgg aaaaatattt tgcttgggaa atggtcaggc tggactgaac aaaggattat
     5401 catcccctcc ttgtccgcca tatccgaaaa tatggaaggc aattccccgg gccaaaacca
     5461 tctatccgca taaaattacc ttgttcccga tactatgccg ctacccgacc tgaccgatgc
     5521 cgaattaata gagtcgcgta aactgcttct gcattttgcg cggcttcagt tgcccgacca
     5581 ccctgatttg gctgaagatt tagtgcagga aacattgctg tccgcataca gcgcaggcga
     5641 cagttttcaa ggcagggcac ttgtcaacag ctggcttttt gccatattga aaaacaaaat
     5701 tattgacgca ttacgtcaaa tcggaaggca gaggaaagtc tttaccacac tggatgacga
     5761 gctactggat gaagcatttg aaagccattt ttcccaaaac gggcattgga cgccggaagg
     5821 gcagccgcaa cattggaaca ctccggaaaa atcattaaac aacaacgaat tccaaaaaat
     5881 tctgcaaagc tgcctataca acctgcctga aaacaccgca cgggtattta ccctgaagga
     5941 aatactcggt ttttcatccg acgaaataca acaaatgtgc ggtatcagca cgtccaacta
     6001 ccacaccatt atgcaccgcg cccgagaatc attgcgccaa tgcctgcaaa tcaaatggtt
     6061 caaccaagaa aacccgaagt aaacgttatg aaaaaatgcc gcgatatcgc cctgcttctt
     6121 tccaaacatc aggaccggga aaccaccccg ggcgagaaga tttccatata cacacacctg
     6181 ctgttctgtc cgtattgccg tgaatataaa agacaacttc aaaccatcaa aagatcactg
     6241 gcaaaaacaa ccagaacttc aaaataaatg ccgtctgaaa aggcttcaga cggcataagc
     6301 tgacggaaac aaatcaaacc gatttactgt tatcggcagt tcatccataa tacacgcttc
     6361 aaaagcagca tattcctcca tacggaatat ataaatacgc aaaacacgaa ggctgcatca
     6421 atttgcttta tttgccttat ttcacagacg gcgctacccc tcccgcccaa cccgttcttt
     6481 ctgaatgagc agatttcaat gattaaggaa accctaatgc gcccaatctt cctatctttc
     6541 gttttactcc ctattttgat aaccgcctgc agcacaccgg acaagtctgc ccgatgggaa
     6601 aatatcggca caatctcaaa cggcaatatt catacatata tcaataaaga cagcgtgaga
     6661 aaaaacggaa atctgatgat tttccaagat aaaaaagttg ttaccaatct aaaacaagaa
     6721 cgttttgcca acacccccgc atacaagact gccattgccg agtgggaaat ccactgcaac
     6781 aacaaaacat accgcttaag ttcgctacag ttgtttgata caaaaaacac ggaaatttcc
     6841 acacaaaact acacagcctc ttccctccgc ccgatgagca tcctgtccgg gacattaacc
     6901 gaaaaacaat atgaaaccgt atgcggaaaa aaactctgat tgcaacttat acacaaactt
     6961 acccacaaac cttatcataa aaatgccgtc tgaaatactg aaatatcagc atttcagacg
     7021 gcattttgcc attccctgaa aattatccac aaagttatcc acattatttt ttaaaaccgg
     7081 cttccatccg aaatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
     7141 gtacaaatag tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa tccactatat
     7201 aaactcgcta tacaatttca ctatccaaac gtaaattgtt ccattgatac acaaaactgc
     7261 ttacccccat aattttgata aagcatttct tacattcccg gctccgtccc gtaaccaaca
     7321 cagcggcgga ttcgcatttg aagtgcaact ttccctaaca gaaaaaggcc agtatgcggt
     7381 agcatacg
//
LOCUS       DAWHRS010000104         7337 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWHRS010000104 DAWHRS010000000
VERSION     DAWHRS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.104"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            66..260
                     /locus_tag="WIB82_001543"
     CDS             66..260
                     /locus_tag="WIB82_001543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842825.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            430..954
                     /gene="nspA"
                     /locus_tag="WIB82_001544"
     CDS             430..954
                     /gene="nspA"
                     /locus_tag="WIB82_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ3842826.1"
                     /translation="MKKALAALIALALPAAALAEGASSFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGMRVKF"
     gene            1090..1479
                     /locus_tag="WIB82_001545"
     CDS             1090..1479
                     /locus_tag="WIB82_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ3842827.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1556..2386
                     /locus_tag="WIB82_001546"
     CDS             1556..2386
                     /locus_tag="WIB82_001546"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ3842828.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2401..3858
                     /locus_tag="WIB82_001547"
     CDS             2401..3858
                     /locus_tag="WIB82_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ3842829.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3899..4288)
                     /locus_tag="WIB82_001548"
     CDS             complement(3899..4288)
                     /locus_tag="WIB82_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842830.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(4470..4871)
                     /locus_tag="WIB82_001549"
     CDS             complement(4470..4871)
                     /locus_tag="WIB82_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842831.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(5288..5617)
                     /locus_tag="WIB82_001550"
     CDS             complement(5288..5617)
                     /locus_tag="WIB82_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842832.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVIPLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(5643..6350)
                     /locus_tag="WIB82_001551"
     CDS             complement(5643..6350)
                     /locus_tag="WIB82_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ3842833.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSV
                     KSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLNKTQTFNNGSAEYHGHVRSW
                     QDLHTDQKNALKKAGLD"
     gene            complement(6485..6802)
                     /locus_tag="WIB82_001552"
     CDS             complement(6485..6802)
                     /locus_tag="WIB82_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842834.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(6805..>7337)
                     /gene="mafB"
                     /locus_tag="WIB82_001553"
     CDS             complement(6805..>7337)
                     /gene="mafB"
                     /locus_tag="WIB82_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ3842835.1"
                     /translation="DQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDF
                     SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKST
                     LNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKN
                     GVHIVPANPKTIKKVQ"
BASE COUNT         1810 a         2028 c         1521 g         1978 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaacc gtatgcctgc acctgcaaca
      181 atccgacaga taccgctgtt ttttccaaac cgtttgcaag tttcacccat ccgccgcgtg
      241 atgccgccgc caccatttaa aggcaacgcg cgggttaacg gctttgccgt cggcaaagca
      301 gccggatacc gctacgtatc ttgaagtatt gaaaatatta cgatgcaaaa gaaaatttaa
      361 gtataataca gcaggattct ttaacggatt cttaacaatt tttctaactg accataaagg
      421 aaccaaaata tgaaaaaagc acttgccgca ctgattgccc tcgctctccc ggccgccgca
      481 ctggcggaag gcgcatccag cttttacgtc caagccgatg ccgcacacgc aaaagcctca
      541 agctctttag gttctgccaa aggcttcagc ccgcgcatct ccgcaggcta ccgcatcaac
      601 gacctccgct tcgccgtcga ttacacgcgc tacaaaaact ataaagcccc atccaccgat
      661 ttcaaacttt acagcatcgg cgcgtccgcc atttacgact tcgacaccca atcgcccgtc
      721 aaaccgtatc tcggcgcgcg cttgagcctc aaccgcgcct ccgtcgactt gggcggcagc
      781 gacagcttca gccaaacctc caccggcctc ggcgtattgg cgggcgtaag ctatgccgtt
      841 accccgaatg tcgatttgga tgccggctac cgctacaact acatcggcaa agtcaacact
      901 gtcaaaaacg tccgttccgg cgaactgtcc gccggtatgc gcgtcaaatt ctgatatgcg
      961 ccttattctg caaaccgccg agccttcggc ggttttgttt tctgccaccg caactacaca
     1021 agccggcggt tttgtacgat aatcccgaat gctgcggctt ctgccgccct attttttgag
     1081 gaatccgaaa tgtccaaaac catcatccac accgacaaag cccccgccgc catcggcgcg
     1141 tacagccaag ccgtccgagc aggcgacacc gtttacatga gcggtcaaat ccccctcgat
     1201 cccgccacga tgaccgtcgt cggcaacggc gatttccgcg ccgaagcgcg ccaagtgttc
     1261 caaaacctgc aagccgtcgc cgaagcggca ggcggcacgc tggccgacat cgtcaaactc
     1321 aacgcctacc tgaccgactt gggcaatttt gccgtcttca acgaagtcat ggcggaattt
     1381 atcgccgagc ccttccccgc ccgcgccgcc gtcggcgttg cctcgctgcc caaaggcgtg
     1441 caggtcgaag ccgaagccgt cctcgttttg aacgcataat tccacgttcg gacggcataa
     1501 gatttccaac cgtgccgtcc gaaccgcaac actttcccac ttcacaacat ctgcaatggc
     1561 acattacgca atcggcgaca tacaaggctg tttcgacgaa ctgaccgcgc tgctcggcaa
     1621 aatcggtttc aaccacggca cggacaccct ctggctgacg ggcgacatcg tcaaccgcgg
     1681 cccgaaatcc ctcgaaacgc tgcaattctg catccggcac gaaaacagcg tgcaaatcgt
     1741 cctcggcaac cacgacctgc acctgctcgc tgtcggctgc ggcgaaggcg cgcccaaacg
     1801 cagcgacaca atcgaaccca tactcaaaca ccccgacgga aaaaaaatgc tcgactggct
     1861 gcgtgcgcaa ccgctgttga tacgcgaggg cagccgcgtg atggtacacg ccggcatcct
     1921 gccgcaatgg cgcatcgcca aagccgaatc gctcgctaga gaagccgaag ccgaactgcg
     1981 cggcaaaaaa tacgtcaaat tcttctccaa aatgtacggc aacaaaccgg ccgcgtggga
     2041 cgaaggtttg gaaggctatg cccgcctgcg cttcatcgtc aacgccttca cgcggatgcg
     2101 cgccctgacc tttaaaaacg aactggattt cgactacaaa tccacagtga aaaaaatgcc
     2161 gccttacctg cgcccgtggt tcaaagcccc cgaccggcaa aacctcgacc gcaccatcat
     2221 cttcggacac tggtcctcgc tgggctacac gaatgccgac aacgtcatct cgctggacac
     2281 cggcgcgctg tggggcggac agctgaccgc cgtcaacctc gaaacggaag aaattaccca
     2341 agtccaagcc gccggcggca tagactggaa aagcttcgca aaataacgga ccgccccact
     2401 atgcacaaaa tcctgcccat cgcccacgtc ctctcccgac tgggtatgct gttttccttt
     2461 atcctgctga tacccgccgc cctctcctac gccttttcgg acggcgcgta caccgccttc
     2521 gccaccaccg cgaccgttac cctttccggc tcgtacatcg tccggctcgc caccctccgg
     2581 ttcaggcgcg aactgcgccc gcgcgacggc ttcaccctcg tcctgatgtt gtggctggcg
     2641 tttgccgcta tggcggcgat gccgatgtac ctgtatttcc cgaatatggg ctttaccgac
     2701 gcattttttg aatcgatgtc gggactgacc accaccggcg cgaccgtcat cccccacgtc
     2761 gacgggctcg ccccctccgt caacttttgg cggcatatgc tcaactggct gggcgggatg
     2821 ggcatcatcg tccttgccgt cgccatcctg cctatgctcg gcgtaggcgg cacgcagtta
     2881 ttcaaagccg aaatccccgg cattgacaaa gaaagcaaaa tgtcgccgcg catttcccaa
     2941 gtggcgaaaa aactttggtt cggctacacc ctgatcacca tcctcgcggc agcctgcctg
     3001 cattttgccg ggatgggctg gttcgatgcc gtctgtcacg cgatggcgac cctctcgctg
     3061 ggcgggtttt ccacccacga tgccagcata gcttattaca actcccccct catcgaagcg
     3121 gtcatcatcg ttttcaccat tgtcggcggc atcaattttg ccaaccattt cgccgccctc
     3181 aacagccgca gcctcaaaac ctattggaaa gacgaagaat gccggacgat gctgctgctg
     3241 ctctccggca gcatccttgc cgccgccctg tacctgtggc acaccggcta ttacgccggt
     3301 tttaccgaat ccctgcgcta caccgccttc aacttcgtct ccatcggatt ggcaaacggg
     3361 ctggccaaca ccgacttcgc acaatggccg ctcctgattt ccctgtggat gtttttcctc
     3421 gccaacatcc tcgccaactc cggctcgacc ggcggcggca tcaaaaccat acgcgccctc
     3481 gtcctgttca aattcagcct gcgcgaaatg atggtgctgc tgcaccccaa agccgtccgc
     3541 accgtcaaaa tcagcggcaa ggccataccc gaccgcctcg cgctgaccgt tatgtccttc
     3601 atcttcatct actttatgac cgtcgtcctc ttcagcttcc tgctgatggc gagcggtatg
     3661 gaatttacca ccgccttcac cgccgtcatc gcctgcatca ccaacgccgg ccccggcctg
     3721 ggcgaagtcg ggcccgccgg caattacgcc ggtttggacg tgatgcagaa atggatctgc
     3781 gttaccgcca tgctcttggg caggctggaa atcttcaccg tcttcatcct cttcaccccc
     3841 gcctactgga agaaataaaa aatgccgtct gaaaactttt cagacggcat attgtctttt
     3901 aaaaaagcgg aagctctaca aaaggaaaga aatgctgaaa cctctgataa atttcaggat
     3961 atttctctaa ggcttttaat gctttttctt ttgaaatagg cggatcatag acatctatcc
     4021 cccttaagaa ggcaatgccc gttaatgcat ttttttgatc atttgaaatg taaccataat
     4081 taacgactcc aattacatca taaatttccc ctatactctt atcaaaatca atcaaatcaa
     4141 aaatatacaa tggaattgta tcaataggag tatctctgac aactatatcc aaccataatt
     4201 taaattcaga aagattaata gcatcataga acatacagac tattgcaaat cctaaatctt
     4261 cacaattttc tttactaatt ttccacataa ttatttacct ttatccattg cttcccaacc
     4321 gatacatccg gtattttagc ttctcctttt ccgtcaaaca ctcctttggt tacctgcttt
     4381 gtgtcaaaaa taccgtctga aagccgaata ttgtttcaga cggcattttg attgttgggt
     4441 ttttaatcag agctttgttt gataatttgt tattttgaat aaaatacagg ttctgtcgag
     4501 ctgataactt ctattacaga atcgctaaaa aagaaaatat aagccttata atatttcttt
     4561 tcaaaaaaat caaaactttc ttctttcaac cagtccatat aactgtcatc tattatctcg
     4621 aaaaaataat ttatagcagg atttctatcc caatcatttt tttctttatt ttcaaataaa
     4681 tattctgaaa attttacttt ataagattca tctgttattt gaaaatgaat tatattgtca
     4741 ataaatattt caatatttaa ttcataattt cggtcggatg ccaaaatgtt cacatcttgt
     4801 tcggtaacac aaattttgtc tacgtaaaat ctgtgattat tgttagaaaa ttgtaatggt
     4861 ttaatcttca ctatcgtttt ccatatctga tttttgtttt cttattgccc tgccggataa
     4921 agcaggtctg cctccgtact gtctatcctc acatttactt tcctgccatc ggcttttatc
     4981 tccccagttt taccgttttt gccaacttcg cggctttggc ggcggcaatg ttgaaggcgg
     5041 cttcgacggt ttcggcggca ttggggttga cccgtactca tccgcaaacg gcaacacgac
     5101 ggacggtgtt tgcttttcag ccgtctgccg ttttgaccaa ggtgctgccg tggaaggagc
     5161 aagtgtagga tttttgaaga actcagttct caatatccta tttcaaatga aagaaatgcc
     5221 gtctgaaaat taaacacggt ttcagacggc attttgattg ttgggttttc aacccgacct
     5281 acggttccta atgattttca gataaaaata aagaaatatc ttctaacgca tcttcagccc
     5341 taccttgaag ctctggatca tcttgctttg aatgtagtaa aggaataact ttatcgtaat
     5401 caccgatatt tgaatgtagc ctggcgagat gtccgaaaca ggttagcgat aatccggcaa
     5461 tatccaagtc tttatttttc gataattcca tcaatttctc agaaatccac ggtacttcat
     5521 atgctttgga attaaatacc atatcaagaa tgccatctat aacactttgt ttatcatcag
     5581 aagaaatcaa cttttctgtt ttacttttat cagtcatttt ttaatttttc cttttgaatt
     5641 aactaatcca atcctgcttt ttttaaagca tttttctgat cggtatgcaa atcttgccaa
     5701 cttctgacat gcccgtgata ttccgcagaa ccgttattaa aagtttgagt tttgtttaat
     5761 acaacgattt cattattggc tttatcaacc ccaactcttc gaggagaagt tgatttaact
     5821 tgaacggaat tatcaagagc tgcttgtccg tttgttggtg ctctactttt cacagaatta
     5881 tcatttttgc catgataaga agcgtcttta tatcgttggt tgcctttatc atacggacaa
     5941 tcattatgaa cccaaacccc ttccgtttcc gcctgactgc ccttgacgaa gtaggtatgc
     6001 caatcggcga cggtcagatt gtaggctttg agcggctgct gtttgaaggt aatgttttga
     6061 accgtctgtt ttgcgccgct ttcggaaagc agggtgtcgc cttttttcag acgacctgcc
     6121 tgtatccatt ttccttgact gtaaaacggg tggattttat tggaaatcag ggtttggttg
     6181 ttgccgatgc cgtctgaaat ttcaatgtaa acggtttctt gatacggatt gccgtatcgg
     6241 gcggtaacgg gtttgtatcc cgtttttccg cttgcctcgt ccttggcgaa gacgcggtcg
     6301 ccggttcgga tacgggcaat ggctttgtag ccgtctgccg ttttgaccaa ggtgctgccg
     6361 tggaaggagc aggtgtagga tttttgaaga actcagttct caatatccta tttcaaatga
     6421 aggaaatgcc gtctgaaaat taaacacggt ttcagaagac attgtgattg ttggtttttt
     6481 taacctacgc ttgcgaaatt aattcctttc tcctgaatac tgcaccattt atttggttta
     6541 actccataga agaatgatta gaataaatac attcaaagtc agcttcatca aaatattctc
     6601 cacacataga ccataactct gtaatcaaat attccatatc ttcatggtct tctgtgatgg
     6661 cttctcttga taaataattt ttgattttta aaaaccttct tccttcctcg taatctacgg
     6721 taattaacct gatatctgag tgtatcatgc ccagcattgc tatattcata ccgactcgca
     6781 gccagcttgg taatatattc ataactattg caccttttta atggttttag ggttagccgg
     6841 aacaatgtga actccatttt tacctgaatg aatcgtacca aaattggtac ttaatcctga
     6901 tttcccatcg ctgcctatag gatacccaaa atcaacaaca ggatttcctc ttgctccttt
     6961 agaaataact ggataagcac ctgaatgtat tccgttcaac aattcttgag gattaatgtt
     7021 ttgatttaaa gtactcttac cttcaatgta gtttctatgt cctgaaatat gtttcccttg
     7081 agctccatca tgaattttag tcccaataga ttttgcttta aaatcaacat tgcgggtttg
     7141 aaacatttct ccatctacag actgagataa tcttgaagca gtgttatgct tgtaggagtc
     7201 tgagaaatcc ccactaaccg cagccttccc cggttttgcc gcctttgtca ggtttttgac
     7261 tttggcaaac ggcaggacgt tgaccagggc ttcgacggtt tcggcggcat tggggttttc
     7321 ctgcatccac tggtcaa
//
LOCUS       DAWHRS010000105         7335 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWHRS010000105 DAWHRS010000000
VERSION     DAWHRS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.105"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1063
                     /gene="dprA"
                     /locus_tag="WIB82_001554"
     CDS             <1..1063
                     /gene="dprA"
                     /locus_tag="WIB82_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ3842836.1"
                     /translation="QVAALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLML
                     LQDEDFPEMLAQGLTAPPVLFLRGNVRLLHKPSAAIVGSRYATPQAMRIAKDFGRALG
                     GKGIPVVSGMASGIDTAAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLI
                     VSEFPIGTRPYAGNFPRRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVP
                     GSIDNPHSKGCHKLIKDGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAV
                     QTAYAPPPAAKMPSEAAAGGTAHGGILDKMGFDPVHPDVLAEQLAMPAADLYAALLEL
                     ELDGSVAAMPGGRYQRIRT"
     gene            1089..1550
                     /locus_tag="WIB82_001555"
     CDS             1089..1550
                     /locus_tag="WIB82_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ3842837.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            1622..3928
                     /gene="topA"
                     /locus_tag="WIB82_001556"
     CDS             1622..3928
                     /gene="topA"
                     /locus_tag="WIB82_001556"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ3842838.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(4001..4696)
                     /locus_tag="WIB82_001557"
     CDS             complement(4001..4696)
                     /locus_tag="WIB82_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842839.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            complement(4716..5267)
                     /locus_tag="WIB82_001558"
     CDS             complement(4716..5267)
                     /locus_tag="WIB82_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842840.1"
                     /translation="MEFLSLYPWWSFFIILTIILSYIGFCAHLHIQNRAVFFYSYRDV
                     TIIFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIITYRSNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            5385..5762
                     /locus_tag="WIB82_001559"
     CDS             5385..5762
                     /locus_tag="WIB82_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3842841.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            5851..6420
                     /gene="rsmD"
                     /locus_tag="WIB82_001560"
     CDS             5851..6420
                     /gene="rsmD"
                     /locus_tag="WIB82_001560"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ3842842.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRHAKRIVIADNNRQTVQTLEKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            6543..6794
                     /locus_tag="WIB82_001561"
     CDS             6543..6794
                     /locus_tag="WIB82_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ3842843.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            6891..6974
                     /locus_tag="WIB82_001562"
     tRNA            6891..6974
                     /locus_tag="WIB82_001562"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6925..6927,aa:Tyr,seq:gta)
     gene            7005..7078
                     /locus_tag="WIB82_001563"
     tRNA            7005..7078
                     /locus_tag="WIB82_001563"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7037..7039,aa:Gly,seq:tcc)
     gene            7088..7162
                     /locus_tag="WIB82_001564"
     tRNA            7088..7162
                     /locus_tag="WIB82_001564"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7120..7122,aa:Thr,seq:ggt)
     gene            7211..>7335
                     /locus_tag="WIB82_001565"
     CDS             7211..>7335
                     /locus_tag="WIB82_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ3842844.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGG"
BASE COUNT         2051 a         1861 c         1812 g         1611 t
ORIGIN      
        1 acaggtggcg gcactgatac ggcacaaaca ggcgcttgag gcttggcgca atgcggaaaa
       61 acgcgccttg gcgcggcagg cggcagaagc ggcgttggaa tgggaaatgc gggacggatg
      121 ccgtctgatg ctgcttcagg atgaagattt ccccgaaatg ctggcgcagg ggctgaccgc
      181 gccgccagtt ttgtttttgc gcggcaacgt gcggctgctg cacaagcctt ccgccgccat
      241 cgtcggcagc cgctatgcca cgccgcaggc gatgcggatt gccaaagatt tcggcagggc
      301 gttgggtggg aaaggcattc ccgtcgtgtc gggtatggct tcgggcatcg acaccgccgc
      361 ccatcagggc gcgttgcagg cagaaggcgg caccatcgcc gtgtggggga cgggcataga
      421 ccgcatttat ccgccgtcca ataaaaacct tgcctatgaa atcgccgaaa aagggctgat
      481 tgtcagcgag ttccccatcg gtacgcggcc gtatgccggc aactttccgc gccgcaaccg
      541 cctgattgcc gcgctgtcgc aggttacgct ggtggttgaa gctgcgttgg aatccggttc
      601 gctgattacc gccggcttgg cggcagaaat ggggcgcgaa gtgatggcgg tacccggttc
      661 gatagacaat ccgcacagca aaggctgcca caaactgatt aaagacggcg cgaaattggt
      721 ggaatgcctg gacgacatcc tgaacgaatg cccggggcta ttgcaaaata cgggtgcttc
      781 atcatattct ataaataagg atacgcccga cacgggacgc cgcgccgttc agacggcata
      841 cgccccgccg cctgccgcaa aaatgccgtc tgaagcggcg gcaggcggca cagcccacgg
      901 cggcatactg gataaaatgg gtttcgaccc cgtccatccc gacgtgcttg ccgagcagct
      961 tgctatgcct gccgcagatt tgtatgccgc actgttggaa ttggaattgg acggcagcgt
     1021 tgccgcaatg cccggcggca gataccagcg tatccgaact tgaacgcact ttatattaag
     1081 gaacacgaat gaccgaagtc atcgcctacc tcatcgaaca tttccaagat ttcgatacct
     1141 gtccgccgcc cgaagacttg ggtatgctgc ttgaagaagc gggtttcgat accatggaaa
     1201 tcggcaacac cctgatgatg atggaagtat tgctcaacag ctccgaattt tccgccgaac
     1261 ctgccgacag cggcgcattg cgcgtgtaca gcaaagaaga aaccgacaac ctgccgcagg
     1321 aagtgatggg gctgatgcag tatctgattg aagaaaaagc cgtcagctgc gaacagcggg
     1381 aaatcatcat ccacgcgctg atgcacatcc ccggcgacga aattaccgta gataccgcca
     1441 aagtgctgac cctgctgctt ttatgggcaa acaagagcga gctgcccgtg ttggtcggcg
     1501 acgagctgat gagcgcgctt ttactcgaca acaaacccac gatgaactga agcggcttca
     1561 gacggcccgc ccgaggccgt ctgaaacgcc ggcatcaaaa ccaccatcca gagaacgaca
     1621 aatggcgaaa aacctattaa tcgtcgaatc cccgtccaaa gccaaaaccc tgaaaaaata
     1681 tttgggcggc gattttgaaa tccttgcatc ctacggacac gtccgcgacc tcgtccccaa
     1741 aagcggcgcg gtcgatcccg acaacggctt tgcgatgaaa taccaactca tcagccgcaa
     1801 cggcaaacac gtcgatgcca tcgtcgccgg tgccaaagaa gctgaaaaca tctacctcgc
     1861 caccgacccg gatagggaag gcgaagccat ttcctggcat cttttggaaa tcctcaaatc
     1921 caaacgcggc ttgaaaaaca tcaagccgca gcgtgtcgtg ttccacgaaa tcaccaaaaa
     1981 cgccgtgctc gatgccgttg cccatccgcg cgaaatcgaa atggacttgg tcgatgcgca
     2041 acaagcccgc cgtgctttgg actatttggt cggctttaac ctttcgccat tgttatggaa
     2101 aaaaatccgc cgcggtttga gcgcgggtcg tgtgcaaagt cccgctttgc gcctgatttg
     2161 cgaacgcgaa aacgaaatcc gcgcgtttga agcgcaggaa tattggacgg tgcatctcga
     2221 cagccacaaa ggccgcagca agttcaccgc caaactcgcc caatacaacg gcgcgaaact
     2281 cgaacaattc gacctgccga acgaagccgc gcaagccgac gtattgaaag aactcgaagg
     2341 caaagaagcc gtcgttaccg ccattgaaaa gaaaaagcgc agccgcaatc ctgccgcgcc
     2401 gttcaccact tctaccatgc agcaggatgc cgtgcgcaaa ctcggcttca ccaccgaccg
     2461 caccatgcgt accgcccaac agctttatga aggtatagac gtagggcagg gcgcgatcgg
     2521 tctgattacc tatatgcgta ccgacagcgt gaatttggcg gatgaagcgt taaccgaaat
     2581 ccgccattac attgaaaaca aaatcggcaa agaatatctg ccgagtgccg ccaaacaata
     2641 caaaaccaaa tccaaaaacg cccaagaagc ccacgaagcc atccgtccga cttccgtgta
     2701 ccgcacgccc gaaagcgtca aacccttcct gagcgcagac cagttcaaac tctatcaaat
     2761 gatttggcag cgcaccgtcg cctgtcagat gacgcctgcc aaattcgacc aaaccaccgt
     2821 cgatattacc gtcggcaaag gcgtattccg cgtaaccgga caagtgcaaa ccttcgcagg
     2881 ctttttgagc gtgtatgaag aaagcagcga tgacgaagaa ggcgaagaca gcaaaaaact
     2941 gcccgaaatg agcgaaggcg ataaattgcc cgtggacaaa ctctacggcg aacaacactt
     3001 taccactccg ccgccgcgct acaacgaagc cacgctggtt aaagccctcg aagaatacgg
     3061 catcggccgc ccctcgacct acgccagcat catctccacg ctcaaagacc gcgaatacgt
     3121 tacccttgag caaaaacgct tcatgcccac cgacacaggc gacatcgtca acaaattcct
     3181 gaccgaacac ttcgcccaat acgtcgatta ccacttcacc gccaaactcg aagaccagct
     3241 tgacgaaatt gccgacggca aacgccaatg gattcccttg atggacaaat tctggaaacc
     3301 gttcatcaaa caagtggaag aaaaagaagg aatcgaacgc gccaaattta ccacgcagga
     3361 acttgatgaa acctgcccga aatgcggcga acacaaactg caaatcaaat tcggcaaaat
     3421 gggtcgtttt gttgcgtgtg ccggttatcc cgagtgcagc tacacgcgca atgtcaacga
     3481 aaccgccgaa gaggccgccg agcgcatcgc caaagccgaa gccgaacagg ccgaactcga
     3541 cggacgcgag tgcccgaaat gtggcggtcg cctagtgtac aaatacagcc gcaccggcag
     3601 caaattcatc ggctgcgcca actatccgaa atgcaaacac gtcgagccgc tggaaaaacc
     3661 gaaagatacc ggcgtccagt gtccgcaatg caaaaaaggc aacctcgtcg agcgcaaatc
     3721 ccgctacggc aaactgtttt acagttgcag cacctatccc gactgcaact acgccacttg
     3781 gaacccgccc gttgccgaag aatgcccgaa ctgccattgg ccggtcttga ccatcaaaac
     3841 caccaaacgc tggggcgtcg aaaaagtctg cccgcaaaaa gaatgcggct ggaaagaaca
     3901 gattgaaccg cccgcgccga aggagtaaga ttaggttggt ttgaaagaga aaaggtcgtc
     3961 tgaaaaattt tcagacgacc tttgcttttc tgtgattggt ttatttgaat ccgcgtgttg
     4021 ttttaaagtc cgataaaatc cggttcattt caggcgcaaa caaggcgatg taatcgtaag
     4081 atagaccgcg actggcactg ggatggggaa agcagacgac ttcgcaatct tcaaacgatt
     4141 ggaatttgac attgaaacgt gtaccgtcaa attctttttg caccgtctcc agcggtttgg
     4201 tctgcttacc gaccaactgc tcgaagcgtg gcagtacatt ttggttgttc agaaaatccg
     4261 ccaacctgct gcccatgaag aggatgactt tcggacgcag tttttcgatg tggtagagaa
     4321 aattatcgat gtgctcgggt tgtgtgaact tgtcgggatt gtcgatagtg ttgccctgtg
     4381 tagcagccca gttggtttga accagggatt tttcaaatgc accgcccaat ccattttcgt
     4441 ctaaggggtg tccccacatt tcaaaccaat tttttatcgt attgtcgtaa cgccacttct
     4501 ttgcctgctc tccgaaatag agggatttgt ttgcaaatgt atggtcgatt ttgttttcag
     4561 ggagtttgta ttcacctgct acataagcag cctcatcggc tttactccaa ccccattcat
     4621 acccacaaat cattaaacca tgtttgtcgt tgtagccttt gaacagactg ttgctcaaat
     4681 tcaaatcctt catcatgaac tcttcctttt aaaatttaag agcgattgac ttcaatgttt
     4741 ttagatgggg tggaaaaatc cttgtgtagg caacataaat tcaataaatt tcttgataat
     4801 tcgaaaccta ctaatagcgc acctataaaa gctttttcat tacgttcagc atgacggtca
     4861 cgtcgttcat attttttacg cttgctgttc cctgttatta cagctaagcc aagtgatatg
     4921 gcgagaattg cccaaacaat agtacttaat aacaattttc cccatactat caataaggaa
     4981 agaaaaaacc ttttagtatt agatcgatag gttataatcc atgcccatga aaatgcaata
     5041 agagttatac ataagataca agctgacagg atttctttat ttttaattaa ataacttaga
     5101 gcgatgagga tgacaggtgt caagaaaata atagttacat cccgatagct ataaaagaaa
     5161 actgccctat tttgaatgtg gagatgtgca cagaatccaa tatagctaag gataatagtt
     5221 aatataataa aaaaagacca ccaagggtaa agagatagga attccatgtt ttcctttttt
     5281 tgtaaaaagg aaaaaatttt atctaagagt taattgccta accagccaga agaagtttaa
     5341 aatctatccc aataattcaa ccatctatac agaaagttca gcttatggaa acccacgaaa
     5401 aaatccgcct gatgcgcgaa ttgaataaat ggtcccagga ggatatggcg gaaaagctgg
     5461 cgatgtcggc aggcgggtat gccaaaatcg aacggggcga aacgcagtta aatatcccgc
     5521 gtttggagca gttggctcag attttcaaaa tcgatatgtg ggacttgctc aaatcgggcg
     5581 gtggtgggat ggtgtttcag attaatgaag gtgatagtgg tggcgatatt gcgttgtatg
     5641 cgtcgggtga tgtttcgatg aaaatagaat ttttaaaaat ggagttgaaa cactgcaaag
     5701 aaatgttgga acaaaaagac aaagaaatcg agctgctccg caagctgacc gaaaccgttt
     5761 aaacagatat gccgtctgaa aaaagttttc agacggcata ttctttgaca ggtcttgtat
     5821 aataccgttt gaacttacag gtttttgatt atggcggcag gcaaacatac caaacacagc
     5881 aaccgggtac gcattatcgg cgggcaatgc cggggcagga aattgagttt cacatccgcc
     5941 gacggactgc gtccgacacc cgacagcgtg cgtgaaaagc tgtttaactg gctgggacag
     6001 gatttgacgg gtaaaacggt tttggatctc ttcggaggca gcggcgcact cggtatggag
     6061 gcggcatcgc gtcatgcaaa acggatcgtg attgcagaca acaaccgtca aacggtacaa
     6121 acccttgaga aaaacagccg cgaactgggt ttggggcagg tgcaaatcgt ctgttcagac
     6181 ggcatcgcat atttgaagac cgtatccgaa cagtttgatg ttgtctttct cgacccgccg
     6241 tttgcatggc aggactggca aatcctgttt gatgccttga agccgtgcct gaacccccga
     6301 gcattcgtct atctcgaggc gggtacgctg ccggatattc ccgattggct gacggaatat
     6361 agagaaggga aatcggggca gagtacattc gaattaaggg ttttccaagt ggctgaataa
     6421 tatgcgcttt gataatcatt tccgagttgt aaacattcgt ttgcaaccgt ccggttcaaa
     6481 aaaaccttgt gctataatcc gcgcccgccc ggttttgata atttagtgga aaaggaaaag
     6541 aaatgtcgct ttttattacc gacgagtgca tcaactgcga cgtatgcgaa cccgaatgcc
     6601 ccaatgatgc catttcccaa ggcgaggaaa tttacgaaat caaccccaac ctctgcacgc
     6661 agtgcgtcgg acactacgat gagccgcagt gccagcaggt ttgcccggtg gactgcatcc
     6721 tgattgacga agaacatccc gaaacccatg acgagttgat ggcgaaatac gaaaagatta
     6781 tccagtttaa ataaattctt tttaaaacat caaattatgt ctgttttgaa ataaaatcaa
     6841 aaaaaaactt gacggaaaag caagccgcta ataaactaac gttctctttt ggagggattc
     6901 ccgagcggtc aaagggggca gactgtaaat ctgttgcgaa agcttcgaag gttcgaatcc
     6961 ttctccctcc accaaaattc ttacttgggg cagtagcgag taatgcgggt gtagctcaat
     7021 ggtagagcag aagccttcca agcttacggt gagggttcga ttcccttcac ccgctccaaa
     7081 caattaggcc catgtagctc aggggtagag cactcccttg gtaagggaga ggccggcagt
     7141 tcaaatctgc ccatgggcac catctctcga ttattcattt ctttaaggct tagatatata
     7201 ggatattgcc atggctaagg aaaaattcga acgtagcaaa ccgcacgtaa acgttggcac
     7261 catcggtcac gttgaccatg gtaaaaccac cctgactgcc gctttgacta ctattttggc
     7321 taaaaaattc ggtgg
//
LOCUS       DAWHRS010000106         7264 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWHRS010000106 DAWHRS010000000
VERSION     DAWHRS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.106"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(77..1423)
                     /gene="mnmE"
                     /locus_tag="WIB82_001566"
     CDS             complement(77..1423)
                     /gene="mnmE"
                     /locus_tag="WIB82_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ3842845.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RTATYADFTDTDGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1556..1711
                     /locus_tag="WIB82_001567"
     CDS             1556..1711
                     /locus_tag="WIB82_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842846.1"
                     /translation="MKKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1708..2139
                     /locus_tag="WIB82_001568"
     CDS             1708..2139
                     /locus_tag="WIB82_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ3842847.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARCFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISAILIL
                     LFAVFLVWSGYPATAASLAGGTVFALAGAFVIGRSRDQGKN"
     gene            2273..6646
                     /gene="app"
                     /locus_tag="WIB82_001569"
     CDS             2273..6646
                     /gene="app"
                     /locus_tag="WIB82_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ3842848.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFTYKIVKRNNYKAGTKGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFND
                     DNNGTGKINAKHEHNSLPNRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTKTDISGNVDLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     NLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFR
                     FMSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFT
                     LQNEHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALI
                     AAGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATT
                     AFPRARRARRDLPQLQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFA
                     EDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENT
                     FDDGIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGGKIRRRVLHYGIQ
                     ARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPA
                     QHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAA
                     AAKGPQLEAQHSAGIKLGYRW"
     gene            complement(6962..7123)
                     /locus_tag="WIB82_001570"
                     /pseudo
     CDS             complement(6962..7123)
                     /locus_tag="WIB82_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1929 a         2087 c         1757 g         1491 t
ORIGIN      
        1 ccaaacggat cggattcccg tttttatggg ataacggaat gttcagccgg acaccaccac
       61 gatgctttcg atccgtttat tttccgatac aaaacctcga aaaaatcacg ccgagcaggt
      121 cgtccgccgt aaactcgccc gtgatttcgc cgcatgcgac ctgcgccaag cgcaagtgtt
      181 cggcaaacag ctcgatttga tggttgccgc acaatgccgc cagcgacaat tcttcctgcg
      241 ctgctttgag tgcgttgacg tgccgcgtcc gcgccaaaaa caacccttcg ctttcgccct
      301 gccaaccggc ctcgcgcaac aacgtccgtt tcagcgcgtc caagccgtcg ccggttttcg
      361 ccgacaacgc gatgacggtt tccgcgcccg taccgaaccc gcctgccgcg tgtgcgtgca
      421 aatcggattt gctgtggatt tcgatgcgtt tcaactccgg cggcaacgcg tccaaaatcg
      481 cccgtgtctt ttcattcaaa ccctcgcgcg gatcgaccaa caccagcgcg acatcggctt
      541 cggatacggc tttgcggctg cgttcgatgc cgatacgctc gaccacgtcg tccgtctcgc
      601 gcaaacctgc cgtatcgaca atatgcaccg gcacgccgtc aatcaggata cgttccctga
      661 ccgcgtcgcg cgtcgttccg gcaatatcgg taacaatcgc cacttcgtcg cccgccaacg
      721 cgttcagcag gctggacttg cccacattcg gcgcgccgac caatacgaca ttcagacctt
      781 cgcgcaaaat cgcgccctgc tgcgcgttgg caagcacatc atccacggcg cggcgcaagc
      841 cgtccagttt gccgcgtgcg tctgccgctt cgagaaaatc aatgtcttcc tcgggaaaat
      901 ctaacgtcgc ttcgaccagc atccgcaagg taatcaagtc ttcgaccaga ccgtgtatcc
      961 gccgcgaaaa atcgcccttg agcgagcgca aagccagacg cgccgccgaa cggctggatg
     1021 cgtcaatcaa atccgccacg ccttccgcct gtgccaagtc cagtttgtcg ttcaaaaacg
     1081 cacgcttcgt aaactcgccc ggttcggcaa ggcgcgcgcc caattccaaa cagcggttca
     1141 gcagcatatc catcaccacc ggcccgccgt gtccctgaag ctcgatgaca tcttcacccg
     1201 taaaacttgc cggtgcggca aaaaacagca aaagcccgct gtcgattgcc tgtccgtccg
     1261 tgtccgtaaa atcagcatag gttgcggtac gcggcttggg cgttttcccg cacaaagcct
     1321 gcgccatcgg cagcaggttt ttccccgata tgcgtatcac gcccacgccg ccgcgccctg
     1381 gtgcggtagc gactgccgca atcgttggaa cgttatccga cataaaaccc ccgaaaattc
     1441 aaaacagccg cgattatagc aaatgccgtc tgaagtccga cggtttggct ttcagacggt
     1501 ataaaaccgc aaaaatgctt gataaatccg tccgcctgac ctaatatata accatatgaa
     1561 aaaacgaaac acatacgccc tcctgctcgg tataggctcg ctgctgggtc tgttccatcc
     1621 cgcaaaaacc gccatccgcc ccaatcccgc cgacgatctc aaaaacatcg gcggcgattt
     1681 tcaacgcgcc atagagaaag cgcgaaaatg accgaaaacg cacaggacaa ggcgcggcag
     1741 gctgtcgaaa ccgtcgtcaa atccccggag cttgtcgagc aaatcctgtc cgacgagtac
     1801 gtgcaaataa tgatagcccg gtgtttccat tcgggaccgt tgccgccgcc gtccgacttg
     1861 gcgcaataca acgacattat cagcaacggg gcagaccgca ttatggcaat ggcggaaaaa
     1921 gaacaagccg tccggcacga aaccatacgg caagaccaaa ccttcaacag gcgcgggcaa
     1981 ctgtacggct tcatcagcgc catcctgata ctgctttttg ccgtcttcct cgtatggagc
     2041 ggctaccccg caaccgccgc ctcccttgcc ggcggcacag tgtttgcctt ggcgggtgct
     2101 ttcgtgattg gaagaagccg agaccaaggc aaaaattaat gacaaatcct agggcgtgct
     2161 tcatatccgc ccgaacgccg aaccgcacat ataggcacat cccgcgcgcc gcccggaagc
     2221 ggaagccgcg ccctcccaaa caaacccgaa tcccgtcaga taaggaaaaa taatgaaaac
     2281 aaccgacaaa cggacaaccg aaacacaccg caaagccccg aaaaccggcc gcatccgctt
     2341 ctcgcctgct tacttagcca tatgcctgtc gttcggcatt cttccccaag cctgggcggg
     2401 acacacttat ttcggcatca actaccaata ctatcgcgac tttgccgaaa ataaaggcaa
     2461 gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa aaaggggagt tggtcggcaa
     2521 atcaatgaca aaagccccga tgattgattt ttctgtggtg tcgcgtaacg gcgtggcggc
     2581 attggtgggc gatcaatata ttgtgagcgt ggcacataac ggcggctata acaacgttga
     2641 ttttggtgcg gaaggaagaa atcccgatca acatcgtttt acttataaaa ttgtgaaacg
     2701 gaataattat aaagcaggga ctaaaggcca tccttatggc ggcgattatc atatgccgcg
     2761 tttgcataaa tttgtcacag atgcagaacc tgttgaaatg accagttata tggatgggcg
     2821 gaaatatatc gatcaaaata attaccctga ccgtgttcgt attggggcag gcaggcaata
     2881 ttggcgatct gatgaagatg agcccaataa ccgcgaaagt tcatatcata ttgcaagtgc
     2941 gtattcttgg ctcgttggtg gcaatacctt tgcacaaaat ggatcaggtg gtggcacagt
     3001 caacttaggt agtgaaaaaa ttaaacatag cccatatggt tttttaccaa caggaggctc
     3061 atttggcgac agtggctcac caatgtttat ctatgatgcc caaaagcaaa agtggttaat
     3121 taatggggta ttgcaaacgg gcaaccccta tataggaaaa agcaatggct tccagctggt
     3181 tcgtaaagat tggttctatg atgaaatctt tgctggagat acccattcag tattctacga
     3241 accacgtcaa aatgggaaat actcttttaa cgacgataat aatggcacag gaaaaatcaa
     3301 tgccaaacat gaacacaatt ctctgcctaa tagattaaaa acacgaaccg ttcaattgtt
     3361 taatgtttct ttatccgaga cagcaagaga acctgtttat catgctgcag gtggtgtcaa
     3421 cagttatcga cccagactga ataatggaga aaatatttcc tttattgacg aaggaaaagg
     3481 cgaattgata cttaccagca acatcaatca aggtgctgga ggattatatt tccaaggaga
     3541 ttttacggtc tcgcctgaaa ataacgaaac ttggcaaggc gcgggcgttc atatcagtga
     3601 agacagtacc gttacttgga aagtaaacgg cgtggcaaac gaccgcctgt ccaaaatcgg
     3661 caaaggcacg ctgcacgttc aagccaaagg ggaaaaccaa ggctcgatca gcgtgggcga
     3721 cggtacagtc attttggatc agcaggcaga cgataaaggc aaaaaacaag cctttagtga
     3781 aatcggcttg gtcagcggca ggggtacggt gcaactgaat gccgataatc agttcaaccc
     3841 cgacaaactc tatttcggct ttcgcggcgg acgtttggat ttaaacgggc attcgctttc
     3901 gttccaccgt attcaaaata ccgatgaagg ggcgatgatt gtcaaccaca atcaagacaa
     3961 agaatccacc gttaccatta caggcaataa agatattgct acaaccggca ataacaacag
     4021 cttggatagc aaaaaagaaa ttgcctacaa cggttggttt ggcgagaaag atacgaccaa
     4081 aacgaacggg cggctcaacc ttgtttacca gcccgccgca gaagaccgca ccctgctgct
     4141 ttccggcgga acaaatttaa acggcaacat cacgcaaaca aacggcaaac tgtttttcag
     4201 cggcagacca acaccgcacg cctacaatca tttaaacgac cattggtcgc aaaaagaggg
     4261 cattcctcgc ggggaaatcg tgtgggacaa cgactggatc aaccgcacat ttaaagcgga
     4321 aaacttccaa attaaaggcg gacaggcggt ggtttcccgc aatgttgcca aagtgaaagg
     4381 cgattggcat ttgagcaatc acgcccaagc agtttttggt gtcgcaccgc atcaaagcca
     4441 cacaatctgt acacgttcgg actggacggg tctgacaaat tgtgtcgaaa aaaccattac
     4501 cgacgataaa gtgattgctt cattgactaa gaccgacatc agcggcaatg tcgatcttgc
     4561 cgatcacgct catttaaatc tcacagggct tgccacactc aacggcaatc ttagtgcaaa
     4621 tggcgataca cgttatacag tcagccacaa cgccacccaa aacggcaacc ttagcctcgt
     4681 gggcaatgcc caagcaacat ttaatcaagc cacattaaac ggcaacacat cggcttcggg
     4741 caatgcttca tttaatctaa gcgaccacgc cgtacaaaac ggcagtctga cgctttccgg
     4801 caacgctaag gcaaacgtaa gccattccgc actcaacggt aatgtctccc tagccgataa
     4861 ggcagtattc cattttgaaa gcagccgctt taccggacaa atcagcggcg gcaaggatac
     4921 ggcattacac ttaaaagaca gcgaatggac gctgccgtca ggcacggaat taggcaattt
     4981 aaaccttgac aacgccacca ttacactcaa ttccgcctat cgccacgatg cggcaggggc
     5041 gcaaaccggc agtgcgacag atgcgccgcg ccgccgttcg cgccgttcgc gccgttccct
     5101 attatccgtt acaccgccaa cttcggtaga atcccgtttc aacacgctga cggtaaacgg
     5161 caaattgaac ggtcagggaa cattccgctt tatgtcggaa ctcttcggct accgcagcga
     5221 caaattgaag ctggcggaaa gttccgaagg cacttacacc ttggcggtca acaataccgg
     5281 caacgaacct gcaagcctcg aacaattgac ggtagtggaa ggaaaagaca acaaaccgct
     5341 gtccgaaaac cttaatttca ccctgcaaaa cgaacacgtc gatgccggcg cgtggcgtta
     5401 ccaactcatc cgcaaagacg gcgagttccg cctgcataat ccggtcaaag aacaagagct
     5461 ttccgacaaa ctcggcaagg cagaagccaa aaaacaggcg gaaaaagaca acgcgcaaag
     5521 ccttgacgcg ctgattgcgg ccgggcgcga tgccgtcgaa aagacagaaa gcgttgccga
     5581 accggcccgg caggcaggcg gggaaaatgt cggcattatg caggcggagg aagagaaaaa
     5641 acgggtgcag gcggataaag acaccgcctt ggcgaaacag cgcgaagcgg aaacccggcc
     5701 ggctaccacc gccttccccc gcgcccgccg cgcccgccgg gatttgccgc aactgcaacc
     5761 ccaaccgcag ccccaaccgc agcgcgacct gatcagccgt tatgccaata gcggtttgag
     5821 tgaattttcc gccacgctca acagcgtttt cgccgtacag gacgaattag accgcgtatt
     5881 tgccgaagac cgccgcaacg ccgtttggac aagcggcatc cgggacacca aacactaccg
     5941 ttcgcaagat ttccgcgcct accgccaaca aaccgacctg cgccaaatcg gtatgcagaa
     6001 aaacctcggc agcgggcgcg tcggcatcct gttttcgcac aaccggaccg aaaacacctt
     6061 cgacgacggc atcggcaact cggcacggct tgcccacggc gccgttttcg ggcaatacgg
     6121 catcgacagg ttctacatcg gcatcagcgc gggcgcgggt tttagcagcg gcagcctttc
     6181 agacggcatc ggaggcaaaa tccgccgccg cgtgctgcat tacggcattc aggcacgata
     6241 ccgcgccggt ttcggcggat tcggcatcga accgcacatc ggcgcaacgc gctatttcgt
     6301 ccaaaaagcg gattaccgct acgaaaacgt caatatcgcc acccccggcc ttgcattcaa
     6361 ccgctaccgc gcgggcatta aggcagatta ttcattcaaa ccggcgcaac acatttccat
     6421 cacgccttat ttgagcctgt cctataccga tgccgcttcg ggcaaagtcc gaacacgcgt
     6481 caataccgcc gtattggctc aggatttcgg caaaacccgc agtgcggaat ggggcgtaaa
     6541 cgccgaaatc aaaggtttca cgctgtccct ccacgctgcc gccgccaaag gcccgcaact
     6601 ggaagcgcaa cacagcgcgg gcatcaaatt aggctaccgc tggtaaccgc cggatatgcc
     6661 gaaaggggtc tgacgatgcc accgtgcgct gtcaaaccct ttttctgccg ccgccttgtg
     6721 cctgctccat tgtctgataa ccgtcaaatt gatttttagc ccatgtttgg tgggtcgggg
     6781 aaatctatat cttcgtccgt gccgaaatag tctgagacct ttgcaaaatt cctttccctc
     6841 ccgacagccg aaacccaaac acaggttttc gtctattttc gccccaaata cctcctaatt
     6901 ctacccaaat acccccttaa tcctccacgg acacccgata atcaggcatc cgggctgctt
     6961 tttaggcggc agcgggcgca cttagcctgt tggccgcttt caaaaggttc aaacacatcg
     7021 ccttcagatg gctttgcgca ctcactttaa tcagtccgaa ataggctgcc cgagcgtagc
     7081 ggaatttacg gtgcagcgta ccgaagctct gttcgaccac atatagtgga ttaaatttaa
     7141 accagtacgg cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct
     7201 tgtcctgatt taaatttaat ccactataac gggtcttcga caaataccgg ttgcgtttgg
     7261 tttg
//
LOCUS       DAWHRS010000107         7235 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWHRS010000107 DAWHRS010000000
VERSION     DAWHRS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.107"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(125..1072)
                     /locus_tag="WIB82_001571"
     CDS             complement(125..1072)
                     /locus_tag="WIB82_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ3842849.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(1087..1494)
                     /locus_tag="WIB82_001572"
     CDS             complement(1087..1494)
                     /locus_tag="WIB82_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ3842850.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVRKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            1575..2234
                     /gene="ung"
                     /locus_tag="WIB82_001573"
     CDS             1575..2234
                     /gene="ung"
                     /locus_tag="WIB82_001573"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ3842851.1"
                     /translation="MDTWHDALGNEKHQPYFQKILNAVRQERLSGQIIYPPETDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDINGFSI
                     PDHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLAAHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            2337..2984
                     /locus_tag="WIB82_001574"
     CDS             2337..2984
                     /locus_tag="WIB82_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ3842852.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            3057..3530
                     /locus_tag="WIB82_001575"
     CDS             3057..3530
                     /locus_tag="WIB82_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ3842853.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            3518..3832
                     /locus_tag="WIB82_001576"
     CDS             3518..3832
                     /locus_tag="WIB82_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEZ3842854.1"
                     /translation="MVHLSEIAAQDYNLNLPRYIDSGEVEDLQNLAAYLYGGIPAHDM
                     DALEAYWQVLGRMKNELFAEHDGRFTTIQRNRLQIFPTLNSLKSCGIGVEFRANLVQP
                     QI"
     gene            3874..4818
                     /locus_tag="WIB82_001577"
     CDS             3874..4818
                     /locus_tag="WIB82_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ3842855.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            4890..5093
                     /locus_tag="WIB82_001578"
     CDS             4890..5093
                     /locus_tag="WIB82_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ3842856.1"
                     /translation="MPTKTFRGSRFGCPHRLIRRVHELTHLLERHRNARFMAHMDNFL
                     PNWQSIKQQLNALELFAQIYNLT"
     gene            complement(5383..5898)
                     /locus_tag="WIB82_001579"
     CDS             complement(5383..5898)
                     /locus_tag="WIB82_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842857.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(6364..>7235)
                     /locus_tag="WIB82_001580"
     CDS             complement(6364..>7235)
                     /locus_tag="WIB82_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3842858.1"
                     /translation="KSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQNP
                     VWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPNLL
                     LLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNFDG
                     DLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAEKE
                     MAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLAKVKTQLAQIEEVWLACQEELE
                     RIETEIEKQFAER"
BASE COUNT         1796 a         1772 c         1823 g         1844 t
ORIGIN      
        1 gcaacgccgt actggttttt gttaatccac tatatttgaa gcggaataca accttgcccg
       61 ggtttaaata aaaatgccgt ctgaaccccg aaaactggac ttcagacggc attttcatca
      121 cggcttattt ggcggttttg ctgctgtcga taattttcat accggcagaa atcaggctgc
      181 cgatgtcggc aacattggcg ggcataatca gcgtattgct ttctttggca agattgttga
      241 acgcagcgac gtattgttcc gcaatcttca gattgaccgc atccgcaccg ccttgggttt
      301 gaagggcggc ggcaatttga cggatggctt cggcattggc ttcggcaaca aggcgcaagg
      361 attccgcttc acctttggcg cggttgatgc gggcgatttt ctcggcattt gacgcattga
      421 ccgcagcctg agcctcgcct tcggattgtt ggatttcggc ttcgcgctga ccactggcaa
      481 ggttgatttg ttcgatttta cgaccttcgg attcggcgat acgggcgcgt ttttcgcgtt
      541 cggcagtaat ttgcgcctgc attgagcgaa ggatttcttg cggcggaacc aagtctttaa
      601 tctcataacg caaaaccttc acaccccaag ccccggccgc ctcgtccaaa gccgcaacaa
      661 cagtactgtt gatttcgtcg cgttcttcaa acgttttgtc caactccata cgcccgataa
      721 cggaacgcag cgtcgtttgg gcaagctggg taatcgccat aatgtagttg ctcgaaccgt
      781 atgaggcgag tttggggtcg gttacttgga aatagatgat gccgtcaaca gtcagctgcg
      841 tattgtcgcg cgtgatgcag acctggctgg gtacgtctaa agggatttct ttcagcgaat
      901 ggcggtaggc gacgcggtcg ataaagggaa tcaaaatatt caaaccggcc gtcagggcgc
      961 gatggaaacg ccccagcctt tcgacaacgt ggacttcctg ttgtgggatg acaacaaagg
     1021 atttgaaacc gaaaacggcg acggctacca acaagataat gaaaaattcc ataattcctc
     1081 cgagtgttaa gggtgtgtga taataagaag gttgccttcc ttgcggacaa tgagggcgcg
     1141 agttcctggt tcaagctctt cttgccccgt attttgagcc tgccagtgcg taccgcgata
     1201 aaaaacttcg taacggttgc cgcctgtgtg tcggaggatt tcgacatatt gtccggcatc
     1261 caaatcctga tatgaatccg tttcaacttt tctaacggcg gttttggcgt gtacgaacca
     1321 aatacccagc gcggaaagca gagcggcggt caagacggcg gcaggcgtac tgccggtcag
     1381 cccgtaagca atgcccgaac ccgccaaagc cgcgctgaca accaaaagat aaaccgttcc
     1441 cgtcaataat tcgatgatta agacggcaac agcggcaaca aaccatacag tcatacattt
     1501 ccccacaaag tgcgtcgttt gacaaaatca cgcgatatca gcagtatagc cgaatttgaa
     1561 aggatagggc agatatggat acttggcacg atgcactggg caacgaaaaa catcaaccgt
     1621 attttcagaa aattttaaat gcggtcaggc aggaacgttt gtcgggacaa atcatctatc
     1681 cgccggagac tgatgtgttc aacgcattcc gcctgacagc gttcgaccgg gtcaaagtcg
     1741 ttattctcgg acaagatccg tatcacgggg cagggcaggc gcacggtttg gcattttccg
     1801 tccggcaggg tgtccgcata ccgccgtctt tactcaatat ctacaaagaa ttggaaaccg
     1861 acatcaacgg tttttccatt cccgatcacg gctgcctgac ggcttgggcg gagcagggcg
     1921 tattgcttct gaacacggtt ttgacggtgc gtgcaggaca ggcgcattcg cacgcccttt
     1981 taggctggga acgctttacc gataccgtca tcaggcagct tgcggcgcac cgcaagcacc
     2041 ttgtcttcat gttgtggggc gggtatgcgc aacaaaaaag gaaactgata gacagtcaaa
     2101 atcatttgat actgaccgca ccgcatccgt ctcctctgtc ggcatatcgc ggttttttcg
     2161 gctgccgcca tttttcacag gcaaacagct atttgagccg gcacggtatc gatccgataa
     2221 actggaagct gtgaatgccg atatagccgt tgccgccggc gtgttaaaat cgcgtttgat
     2281 ttgtaatttc catttattag gcaaaacctt atggcaatca aaaaaaaatc agctttatgc
     2341 atcactttgg gcgagctgcg acgagttgcg cggcggtatg gatgcgagcc tttataaaga
     2401 actatgtgct tacgctattg tttttaaaat atgtttctga taagcataag tacggcggcg
     2461 gcatgattga gctgcacgcc ggtacgactt ttgacgacat cgtcaaactc aaaaacaccg
     2521 ccgacatcgg cgaccgcctg aataagatta tcgcccaaat tgccgaagcc aacgacttaa
     2581 aaggcgtgat cgacgttacc gacttcaacg acgaagacaa actgggtaaa ggtaaggaga
     2641 tgatcgaccg tttgagcagg cttgtcggca tttttaaaaa gctcaacctt tcttccaacc
     2701 aagccgaaga cgacgatttg ttaggtgatg cctacgaata cctgatgcgc cattttgcga
     2761 ccgagtcagg caaatccaaa gggcagtttt acacgcctgc cgaagtctcc cgcattatgg
     2821 cgaagattat cggaatcagc gcagattgcc gtccagcacc agcgtttatg acccgacctg
     2881 cggctcgggt tcgctgttgc tcaaagccgc cgcccaagcc ggcagccaaa tcagccttta
     2941 cggtcaggaa aaagatgtgg caaccgcgtc ccttgcccgt atgaatatga ttttgcacaa
     3001 caacgaaacc gccgaaatca acaccgggaa caccttgtcc gattcgtctt tccgtgatga
     3061 aaacgacggg cttaagacct tcgattttgc cgttgccaat ccgccttatc ccgcccgaaa
     3121 aaaacggcga ttacgccttt ttgctgcatc tgctcaaaag cctgaaacca agcggcaaag
     3181 gtgcgattat tcttccgcac ggtgtgctgt ttcgcggcaa tgccgaagcg cgtattcgca
     3241 cggaattgct taaccttgac cttattaaag gcattatcgg gctgcctgcc aacctgtttt
     3301 acggcacggg cattcctgcc tgcatcatcg tcatcgacaa agaacacgcc caaaccgccc
     3361 aatttgccga agagggaaca aaccaagtta tcagtggcgg cagcgtgttt atgattgacg
     3421 catcgcgcgg cttcattaaa gacggcaaca aaaaccgtct gcgtgagcaa gacattcaca
     3481 aaatcatcga cactttcaca aacctcgtta cagccgtatg gtgcatttaa gcgaaatcgc
     3541 agcacaagat tacaacctta atctgcctcg ctatatcgac agcggagaag ttgaagacct
     3601 gcaaaatctc gccgcctatc tttatggcgg catacctgcg cacgatatgg acgcattgga
     3661 agcctattgg caagttttag gccgtatgaa aaacgagttg tttgccgaac acgatggccg
     3721 ctttaccact atacaacgga atcgattgca aatctttccc actctcaaca gcttaaaatc
     3781 ctgcgggatt ggtgtggaat ttagggctaa tctagtacag ccccaaattt aatccactat
     3841 aaaatcgaaa gcagccaaat caaagcccat atattggcgc accccgatta cgccgccttc
     3901 aaagccggac acctggcaaa gtttgccgcg tggcacactc aaaacgacct tgccgccatc
     3961 caaccgggca ggcttatccg gaaatggagc gaaagcctgc tggacgcgtt caaacccggc
     4021 agcctgattg aagaatacga tttctaccaa atcctgacgg actactgggc ggaaaccctg
     4081 caagacgatg tttatctcat cgcccaagat ggctggaagg cggttaaaaa cctggccgaa
     4141 atcaccaaag aaagcgatga agacgcgaac ctgaccgtcg tctttgagga aaccgaaacc
     4201 ggcaaaaaag gcaaagccaa aaccaagcgc atcagcaaaa aataccgcag cgaagtcatc
     4261 gcccccgagc tggttgcccg ccgctacttt tcagacggca tcgccaagct ggaagaaaaa
     4321 caaagcgagc tggaacgcct aagccaagaa ttggaaaacc acatagaaga acacggcggc
     4381 gaagagggtg cgctgaacga cgtattggat gcaaaaggca aactttccgc caaacttctg
     4441 aaaaccgcat tggaagaaag cggcatagaa gaaggcgaac gggctgtttt acaaaccacc
     4501 caaacactga tgacgcagga aaaagccgcg aaagacgcag tcaaaaccca aatcgaagcc
     4561 ctgaaccttg ccgtattcaa acaattcggc cgactttccg aagccgaaat caagcagctt
     4621 gccgttcaag acaaatggct tgccgattta caaagccgaa tcgaaaatcg cttggaaaac
     4681 agtattcagc agcttatcag ccgcttgaac acgctggaag accgctaccg cagcccgatg
     4741 gccgagcttg cccgagaagt ggaaaagtgg caaagcaaag tcaatgccca ccttgaaaat
     4801 atgggttttg gaggctgaaa tggcggcaca gacaggctat aaggcgagcg ggttttgaga
     4861 cctttgcaaa attccccaaa atcccctaaa ttcccaccaa gacatttagg ggatcgcggt
     4921 tcgggtgtcc gcaccgctta atacgtcgtg tccacgaact aacccatttg ctcgaacgcc
     4981 atcgcaacgc ccgttttatg gcgcatatgg acaactttct cccaaactgg caaagcatca
     5041 aacaacagct taatgccttg gagttatttg cacaaatata taatttaaca taatatacat
     5101 tatgcgaact atcggaaaca gttgtacgtg tccctgtggt ctttccaagt aggaaaatta
     5161 aagtatggcc aatgcggctg aatgtatagc ccggagcatc cgcgtatccg aaggtactga
     5221 acgacatgcc ctgcatccct ttcgacgctt ccatccagcc ggtattggga aatacgttga
     5281 ccttcaatat aatatacagg cagccgacac acaggatatg ccgctgcttt ttcatcattt
     5341 cttctgtcaa atccttggaa cggtcgattt gaaaacacgg ctttacatac gccccagttc
     5401 cctttgcagg gcttgaatat tttgctgcct gtccaataca ttgctttgta atgcatttat
     5461 ttcttgatgg ttgatgttgc cgccctttgc cagacgtgct tgtgataaca ttttttgggc
     5521 ttcaaccaat gctttgcgtt cgttgctcaa ttctgtttcg agaatggagc gtctgctgtt
     5581 gtttgagggt gcttgttgcg gcggcggcgt attggatttt gccggcttgg atactgtttt
     5641 gaccggggct ttatatttga caacctgtcc gccgtttgac ggtgatgata ccggttcggg
     5701 cgtttggggc gggatatagc gttcgctgct gtagttgccg attgggggca aatcggttga
     5761 gtggcagctt ttggacggct tggtggtgta aacggtttct ccgttgattg tgcaggtgta
     5821 gattttggcc gcattcgcac ccaatgggct tgaaatcagg gaaaagttga ttaggattaa
     5881 ggggagtttt gatttcataa tgtgcttgat gctcggataa ttatttgatt ttttggtatt
     5941 ttttataatg ctttcttggc gttttacggg cttttctttt cggttgtttg cttttgattt
     6001 tctttcgggg ctttggcagc ctgcttgttc tttttcccgg caggtttttt gtttttcttt
     6061 ttactttcca tgtgtatttt ggcttcttaa ctgagttttt taattttcag gcggtatccg
     6121 cctccctgat ggctgctgat tttaggtaaa tccgcatcgg cgcacgatcc tgctggggct
     6181 gatgtatata cgcttttgcc atttgagttg caatggtata cggaggcttg cgccgccgcg
     6241 ccggaaaggg acaggagacc caaggcggca aaaagtctga tgtttatacc ggtaaaaggc
     6301 ggtgataacg cccgaattat accgttattg gcaggcaaag ataagcaccc tgtccgcgct
     6361 tctttatcgc tcggcaaact gtttctcgat ttcagtttca atccgttcca attcttcttg
     6421 acaagccagc caaacctctt cgatttgggc aagttgtgtt ttgacttttg ccagcaggga
     6481 taaggtattt tgcaattttt ctttgtttgc ttcaaggtaa gcgtcttctt gtgctaaaaa
     6541 tgcttcacat gccgtctgaa tttcggaaag ctgcgccatt tctttttcgg cacggtctat
     6601 tttctgctgt atcggcttgc cgcgtcgggc tttttcctga cggatttgcg cttcgatgcg
     6661 cttggtgtct ttgcggcttt ggctttgtgc ggatgctgct ggtgcggcgg cgttttcctg
     6721 tgccaaacgc cattggcggt aatcgttcaa atcgccgtcg aagttcttca gacggccttt
     6781 gtcgatcagg aggaagctgt cggtagtcgc ttcaagcagg ctgcggtcgt gcgatacgat
     6841 gattaaggcg ccttggaaac tttgcaacgc gagcgttaag gcgtggcgca tatccaagtc
     6901 caaatggttg gtcggttcgt caagcagcaa caggttcggc ttttgccaga taatcatggc
     6961 aagggcaagg cgggcttttt ctccgccgga aaaaggttcg gttttctgta acgccatatc
     7021 gccaacaaaa ttgaagccgc cgaggaaatt tcggatttct tgttcgcgta cttcgggaga
     7081 aagctgctga atatgccaaa cagggttttg gtcggcgcgg atggtgtcga gttggtgttg
     7141 ggcaaaatag ccgatattga gtttttcgga acggatgatg ctgccggaaa gcaaatcgat
     7201 tttgcctgcc aaggctttga taaaggtgga tttgc
//
LOCUS       DAWHRS010000108         7202 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWHRS010000108 DAWHRS010000000
VERSION     DAWHRS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.108"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(62..146)
                     /locus_tag="WIB82_001581"
     tRNA            complement(62..146)
                     /locus_tag="WIB82_001581"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Leu,seq:caa)
     gene            422..1075
                     /locus_tag="WIB82_001582"
     CDS             422..1075
                     /locus_tag="WIB82_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ3842859.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1154..1807
                     /locus_tag="WIB82_001583"
     CDS             1154..1807
                     /locus_tag="WIB82_001583"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ3842860.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1911..2810
                     /locus_tag="WIB82_001584"
     CDS             1911..2810
                     /locus_tag="WIB82_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ3842861.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLDEINSNELILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2807..4096
                     /gene="hisD"
                     /locus_tag="WIB82_001585"
     CDS             2807..4096
                     /gene="hisD"
                     /locus_tag="WIB82_001585"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ3842862.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4142..5239
                     /locus_tag="WIB82_001586"
     CDS             4142..5239
                     /locus_tag="WIB82_001586"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ3842863.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALRHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5236..6153
                     /gene="hisB"
                     /locus_tag="WIB82_001587"
     CDS             5236..6153
                     /gene="hisB"
                     /locus_tag="WIB82_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ3842864.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6248..7117
                     /locus_tag="WIB82_001588"
     CDS             6248..7117
                     /locus_tag="WIB82_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ3842865.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1881 a         2118 c         1724 g         1479 t
ORIGIN      
        1 tagtcttatc tatttcatta aacagcaata gacaaaaaaa taaccgctcc aaaagcggtt
       61 gtggtgccca gggtcggact cgaaccgaca caccttgcgg cgggggattt tgagtcccct
      121 gcgtctacca atttcgccac ctgggctggt gaagaagtcg tcattataat ggcttttgaa
      181 attctgtaaa cctttttttg aaattatttt atctgttttt attttatttt tgattttaaa
      241 tagaattttt attattttaa tcttactgtt ctttccgttc caaagattct gtatgattcg
      301 gcaattcctg ccgtgcagac aacgtaaaaa aatactacat taaatctgcc aaacgcgtta
      361 agatggaaat attcaaattc cgtacgaatc aggttttgct atttattctt gggagattgt
      421 catgttttcc gtaccgcgtt cctttttgcc gggcgttttc gtacttgccg cgcttgccgc
      481 ctgcaaacct caagacaaca gtgcggcgca agccgcttct tcaagtgcat ccgcgtcggc
      541 tgcggaaaat gcggcaaagc cgcaaacgcg cggtacggat atgcgtaagg aagacatcgg
      601 cggcgatttc acgctgaccg acggcgaagg caagcctttc agcctgagcg atttgaaagg
      661 caaggtcgtg attctgtctt tcggctttac gcactgtccc gatgtctgcc cgacagagct
      721 tttgacgtac agcgacacgt tgaagcagtt gggcgggcag gctaaggacg tgaaagtggt
      781 gttcgtcagc atcgatccgg aacgcgacac gcctgaaatc atcggcaagt atgccaaaca
      841 gttcaatccg gactttatcg gtctgacggc aacgggcggc caaaacctgc cggtcatcaa
      901 gcagcaatac cgcgtggttt ctgccaaagt caatcaaaaa gacgacagcg aaaactattt
      961 ggtcgaccac tcttccggtg cgtatctcat cgacaaaaac ggtgaggttg ccattttctc
     1021 gccttacgga agcgagccgg aaacgattgc tgccgatgta aggaccctgc tctgataaaa
     1081 ccgtatgccg tctgcaccgt cggcgcctgt tcagacggca ttattgtttc aaccgacaaa
     1141 ggacatccac accatgcagg ataatgcttt gaccatcgcc ttatccaagg ggcgcatttt
     1201 tgaggagacg ctgccgctgc ttgccgctgc cggcattgtt ccgactgaag agcctgaaaa
     1261 atcgcgcaag ctgattatcg ggacgaacca tgaaaacatc cgccttgtca ttgtccgcgc
     1321 aaccgatgtg ccgacttatg tccgctacgg cgcggcggac ttcggcattg cgggcaaaga
     1381 cgtgctgatc gaacacggcg gcacggggct ttaccggcct ttggatttgg agattgccaa
     1441 gtgccgcatg atggttgctg tgcgtaaagg gtttgattac gaagcagctt cgcaacccgg
     1501 atgccgtctg aagattgcca cgaagtatcc tgaaatcgcg gcatctcatt ttgccggcaa
     1561 gggtgtccat gtggacatta tcaaactgta cggctcgatg gaacttgcgc cgctggtcgg
     1621 cttgagcgat gcgattgtgg acttggtttc gacgggcaac accttgaagg caaacggctt
     1681 ggaagcagtc gaacacatcg tcgacatttc cagccgcctg gtggtcaaca aggctgcttt
     1741 gaaaacgaaa tacgcgctgc tggagccgat tattcaggcg ttcggcggcg cagtgaaggc
     1801 gaagtaagca ttcatttgaa taaagatgcg ttttcagacg accctatccg ttcccgccga
     1861 caggtcgtct gaaaatatca ccggcagtaa actgtatagg agaagttaaa atggttgcaa
     1921 aaataaaaaa attctcagat tcaacccttt ccgttttgaa taacggcgag cgtcggtttt
     1981 atgtctattg tctgaccgac ctgaaaaaag acaaaatcct ctacatcggc aaaggctgcg
     2041 gtaatcgtat cttcgagcat gaatgggttg ctagtcgttc acaagaccca gtctccggcg
     2101 agattatcga tcggaaactc aaagccatct ccaaatgcaa gaaactcggt cgctatatca
     2161 tcagctatca tctgactgaa gtcgaagcgc tcgccgccga atctgcctta attcattttg
     2221 ttaaatctgt cttgggtaaa aaactcaaaa ataaaattgc cgggcatggt ccgggtggta
     2281 ttagcgtaga agaactagat cgccgctttg gattctcttc tctcccactt gatgagatca
     2341 actccaacga gctaattctc gccatcaaaa tccacaatgc tttcgattta gatactgacg
     2401 aagaattaga ctaccttttc gacaaccaag acgatgccaa cctcaaatcg cgtacgttgg
     2461 gcaactgggt tatcggtaaa gatgttgctt caaaagtgaa atacgttatc ggcgttcaca
     2521 ccggtctgca aaacgctgtt gtcagtgcat acgaagtgga cggttttgaa acaatggttg
     2581 aggaaaccaa aaacggtaga aaacaatccc gttaccgttt ccgcactacc tctcgtagcg
     2641 aagaggtatt agccaaactc ggtctgcaac aaaaatgcct gcccgaattg aagtttggta
     2701 gcgggggaga aaaagcgtat atcagaccca aaacagagac agaaactgaa caagagaata
     2761 ttcagacgac ccccaatcca aaaataaaaa aggaaaaaac caaatcatga aaaaactcaa
     2821 cacccaatcg cccgatttcc aagccggact caaagccctg ctggcttttg aaaccgcgca
     2881 aaaccccgaa accgaacgca tcgtcgccga catttgcgcc gacgtgcaaa agcgcggcga
     2941 tgcggctttg attgaataca ccaacaaatt cgatcagaca aacgctaaaa gcatcgatga
     3001 tttaatactc acgcaagccg atttgaacgc ggcgttcgag cgcattccga acgacgttca
     3061 gacggcattg cagaccgccg cccgccgtgt cgaaagctac caccaacgcc aaaaaatgga
     3121 atcgtggagc tacaccgatg aagacggcac gctgttggga caacaaatca caccgcttga
     3181 ccgcgtcggc atttacgtcc ccggcggcaa ggcggcgtat ccgagttccg tcatcatgaa
     3241 cgccatgccc gcccacgtcg caggtgtgaa agaaatcatc atggtcgtgc cgacaccaaa
     3301 aggcgaacgc aacgacatcg tacttgccgc cgcatacgtc gccggcgtaa ccaaagtctt
     3361 caccgtcggc ggcgcgcagg cggttgccgc cctcgcctac ggcacggaaa ccatccccca
     3421 agtcgataaa atcaccggtc cgggcaacgc cttcgtcgcc gccgccaaac gccgcgtgtt
     3481 cggcgtggtc ggcatcgaca tggtggcggg gccgtctgaa atcctggtca tcgccgacgg
     3541 cacgacacct gccgattggg tggcgatgga tttgttcagc caggccgaac acgacgaaat
     3601 tgcccaagcc atcctcatcg gcacgtcgca agcgtatctc gacgaagtag aagccgctat
     3661 ggaccgcctg atcgaaacta tgccgcgccg cgacatcatc gaagcctcgc tcggcaacag
     3721 gggcgcgatg atactcgcca aagacttgga cgaagcctgc gaaatcgcca actacatttc
     3781 ccccgaacac ttggaactgt cagtcgaaaa cccgcaggaa tgggcgaaaa aaatccgcca
     3841 cgccggtgcg attttcatgg gacgctacac cggcgaaagc ctcggcgact actgcgccgg
     3901 tccaaaccat gtgttgccca ccagccgaac cgcccgcttt tcctcgcctt tggggacata
     3961 tgatttccaa aaacgctcca gcctgattca ggtttcggaa cagggcgcgc aaaaattagg
     4021 cgaaaccgcc agcgtgctgg cacacggcga aagcctgacc gcccacgccc gcgcggcaga
     4081 gttccgtatg aaataatgcc gaaacggcgt acaggcatat tccaaccatt aaggaaacac
     4141 gatgaaatcc gtccgctcct tcatccgcga cgacatacaa gctatgtcgg catatcagat
     4201 tgccgacgtt ccgcccggct ttgccaaact cgattcgatg gaaagtcccg tccacccttt
     4261 tgccggacat gaaacgctgt tgcaggaatg gcaggcacgg cttgccgccg cgcccatcca
     4321 tctttacccc aatccctccg gcagcggttt acaggaagca ttacgttcgg cgttcgacat
     4381 tcccgactgc gccgacatcg cgctgggcaa cggttcggac gaactgatac agttcatcac
     4441 gatgctgacc gccaaaccgg gcgcggcaat gttggcagcc gaacccagtt tcgtcatgta
     4501 ccgccacaac gccgcgctgt acggcatgga ttatgtcggc gttccactga acggagattt
     4561 caccctcaac ctgcccgccg tcctcgaagc cgtcaggaaa caccgccctg ccctgacctt
     4621 tatcgcctac cccaacaacc ccaccggcgt atgcttcacg cgtgccgaaa tcgaagccgt
     4681 catcgaagct tcagacggca tcgtcgtcgt cgatgaagcc tacggcgcat tcaacggcga
     4741 cagcttcctg ccgcaggcag gcaggattcc caacctgata gtcttacgca ccctcagcaa
     4801 aatcggtttt gccggactgc gtatcggtta tgcggcaggc tgccccgaag tcatcggcga
     4861 actgcaaaaa atcctgccgc cctacaatat gaaccaattg agcctgacca ctgccaaact
     4921 cgccctgcgg cactacggca ttatctctgc caacatcgac agcctgaaaa acgaacgcga
     4981 acggatgttc gccgaattgg gcaaaatatg ccgtctgaac acctttccaa gtcaggcaaa
     5041 cttcattacc atacgcgtac ccgatgccga tttgttgttt gacacgctca aacaaaaccg
     5101 catcttggtt aaaaaactgc atggcgcgca cccgcttttg gaacactgcc tgcgcattac
     5161 cgtaggcagc cccgcacaaa acgatgccgt tctcaacatc attcgccaac tttactgcca
     5221 accaacggat ttcctatgaa tttgactaaa acacaacgcc aactgcacaa ctttctgacc
     5281 ctcgcacaag aagcaggttc gctgtccaag ctcgccaaac tctgcggcta ccgtaccccc
     5341 gtcgcactct acaaactcaa acaacgcctt gaaaagcagg cagaagaccc agatgcacgc
     5401 ggcatccgtc ccagcctgat ggcaaaactc gaaaaacaca ccggcaaacc caaaggctgg
     5461 ctcgacagaa aacaccgcga acgcactgtc cccgaaaccg ccgcagaaag caccggaact
     5521 gccgaaaccc aaattgccga aaccgcatct gctgccggct gccgcagcgt taccgtcaac
     5581 cgcaatacct gcgaaaccca aatcaccgtc tccatcaacc tcgacggcag cggcaaaagc
     5641 aggctggata ccggcgtacc cttcctcgaa cacatgatcg atcaaatcgc ccgccacggc
     5701 atgattgaca tcgacattag ctgcaaaggc gacctgcaca tcgacgacca ccacaccgcc
     5761 gaagacatcg gcatcacact cggacaagca atccggcagg cactcggcga caaaaaaggc
     5821 atccgccgtt acggacattc ctacgtcccg ctcgacgaag ccctcagccg tgtcgtcatc
     5881 gacctttccg gccgccccgg actcgtgtac aacatcgaat ttacccgcgc actaatcgga
     5941 cgtttcgatg tcgatttgtt tgaagaattt ttccacggca tcgtcaacca cagtatgatg
     6001 accctgcaca tcgacaacct cagcggcaaa aacgcccacc atcaggcgga aaccgtattc
     6061 aaagccttcg ggcgcgccct gcgtatggca gtcgaacacg acccgcgcat ggcaggacag
     6121 accccctcga ccaaaggcac gctgaccgca taaaaaacca taccgtctga aacacccgca
     6181 ggcttttcag acggtatcgg aacagataag attacactac actacaaaca gaaaaggagt
     6241 aaacatcatg tccgcaaacg aatacgcaca aatcggctgg ataggcttag ggcaaatggg
     6301 tctgcctatg gtaacgcggc tcttggacgg cggcatcgaa gtcggcgtat acaaccgctc
     6361 gcccgacaaa actgccccca tctccgccaa aggcgcaaaa gtttacggca acaccgccga
     6421 actcgtccgc gactatcccg tcattttcct gatggtttcc gactatgccg ccgtgtgcga
     6481 catcctgaac ggagtccgcg acggattggc cggcaaaatc atcgtcaaca tgagcaccat
     6541 ctccccgacc gaaaacctcg ccgtcaaagc acttgtcgaa gccgcaggcg gacagtttgc
     6601 cgaagcaccc gtttccggat cggtcgggcc cgccaccaac ggcacgctgc tgattctgtt
     6661 cggcggcagc gaagccgttt taaacccgct gcaaaaaata ttttccctcg tcggcaaaaa
     6721 aaccttccat ttcggcgatg tcggcaaagg ttcgggcgcg aaactcgtct tgaactcgct
     6781 cttgggcatt ttcggcgaag cgtacagcga agcgatgctg atggcgcggc agttcggcat
     6841 cgataccgac accatcgtcg aagccatcgg cggctcggca atggactcgc ccatgttcca
     6901 aaccaaaaaa tccctgtggg caaaccgcga attcccgccc gccttcgccc tcaaacacgc
     6961 ctccaaagac ctcaacctcg ccgtcaaaga gcttgaacag gcaggcaaca ccctgcccgc
     7021 cgtcgaaacc gttgctgcca gctaccgcaa agcagtcgaa gccggctacg gcgaacagga
     7081 cgtttccggc gtttacctga aactggcaga acactgattg ccttttccaa acacaatgcc
     7141 gtctgaacat atttcagacg gcatttttat caccccacgc ttaaaatcag tcccgattat
     7201 ga
//
LOCUS       DAWHRS010000109         7149 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWHRS010000109 DAWHRS010000000
VERSION     DAWHRS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.109"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            169..1092
                     /locus_tag="WIB82_001589"
     CDS             169..1092
                     /locus_tag="WIB82_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3842866.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="WIB82_001590"
     CDS             complement(1157..2545)
                     /gene="fumC"
                     /locus_tag="WIB82_001590"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ3842867.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYGNAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(2693..4672)
                     /gene="tkt"
                     /locus_tag="WIB82_001591"
     CDS             complement(2693..4672)
                     /gene="tkt"
                     /locus_tag="WIB82_001591"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ3842868.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            5199..6695
                     /gene="ccoG"
                     /locus_tag="WIB82_001592"
     CDS             5199..6695
                     /gene="ccoG"
                     /locus_tag="WIB82_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ3842869.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            6698..>7149
                     /locus_tag="WIB82_001593"
     CDS             6698..>7149
                     /locus_tag="WIB82_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ3842870.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAIRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAG"
BASE COUNT         1501 a         1742 c         2143 g         1763 t
ORIGIN      
        1 aaaattcagg cggtgttttc aaatagcgcg aagcaatagc cgagcttcag ccggtttgtt
       61 tgaaatcaaa gggttggaat caaacgccaa acgcttgaac catcattgcc cgatgcggca
      121 aaccggcatc gtccggcaat acggttgttt aaaaatagga aaatcaggat ggaaaaccaa
      181 aggccgctcc taggcttcgc gttggcactt ttggcggcga tgacgtgggg aacgctgccg
      241 attgccgtgc ggcaggtatt gaagtttgtc gatgcgccga cgctggtgtg ggtgcgtttt
      301 accgtggcgg cggcggtatt gtttgttttg ctggcactgg gcgggcggct gccgaagcgg
      361 cgggattttt cttggtgctc attcaggctg ctgctgctcg gcgtggcggg catttcggca
      421 aactttgtgc tgattgccca agggctgcat tatatttcgc cgaccacgac gcaggttttg
      481 tggcagattt cgccgtttac gatgattgtt gtcggtgtgt tggtgtttaa agaccggatg
      541 actgccgctc agaaaatcgg cttggttttg ctgcttgccg gtttgcttat gttttttaac
      601 gataaattcg gcgagttgtc gggtttgggc gcgtatgcga agggcgtgtt gctgtgtgcg
      661 gcaggcagta tggcatgggt gtgttatgcc gtggcgcaaa agctgctgtc ggcgcaattc
      721 ggaccgcaac agattctgct gttgatttat gcggcaagtg ccgccgtgtt cctgccgttt
      781 gccaaaccgg cacacatcgg aagtttggac ggtacgttgg cgtgggtttg ttttgcgtat
      841 tgctgcttga atacgttaat cggttacggc tcgttcggcg aggcgttgaa acattgggag
      901 gcttccaaag tcagcgcggt aacaaccttg ctccccgtgt ttaccgtaat attttctttg
      961 ctcgggcatt atgtgatgcc tgatactttt gccgcgccgg atatgaacgg tttgggttat
     1021 gccggcgcac tggtcgtggt cgggggtgcg gttacggcgg cggtggggga caggctgttc
     1081 aaacgccgct agttcgcagg caacggaaaa tgccgtccga acgaggtttc agacggcatt
     1141 ttatttgagg gaaagattag cgcggatgaa ccatatcggc aggaacgacc agttcgtcaa
     1201 attcttcgcc cgtcagcaag cccaactcaa cggcggtttc gcgcaacgat ttgttgtttt
     1261 tgtaggcggt tttggcgact ttggcggcgt ttccgtaacc gattttacgg tttaacgcgg
     1321 taacgagcat cagggaatgg tgcaggaaat agtcgatttt ttccggtacg ggttcgatgc
     1381 cgatggcgca gtgttcgttg aagctgttgc acgcgtcgcc caagaggcgg atggattgca
     1441 agaggttgta ggcgataacg ggcatataga cgttcagctc gaaattgccc gacgcgcccg
     1501 ccatgccgat ggtaacgtcg ttgccgaaca cttggcagca caccatggtc atcgcttcgc
     1561 attgggtcgg gttgactttg cccggcatga tggacgaacc cggctcgttt tcggggattt
     1621 tgatttcgcc caaaccgcag cgcgggccgc ttgccagcca gcggatgtcg ttggcgattt
     1681 tattcaggct tgccgccagc gttttcaatg cgcccgaagc ggcaacggcg gcatcgcgtc
     1741 cgcccaaggc ttcaaatttg ttcggcgcgc tgacaaacgg caagccggac aattcggcga
     1801 gtttggcggc ggctttttcg gcgtattcgg gatggctgtt caaacccgtg ccgaccgccg
     1861 taccgcccaa agcaagttca tacaagtctt taagcgcgtc gttcagacgg cctaagccgt
     1921 ggtcgagctg ggaaacgtag ccggaaaatt cttggcctaa agtcagcggc gtcgcgtcct
     1981 gcaagtgggt gcggccgatt ttgacgatag gggcgaaagc ttgggctttt ttgtccaagg
     2041 tatcgcgcag ggcttttacg gcggggatga ggtggcggtt gatttcaatc gcggcggcaa
     2101 cgtggatagc ggtcgggaat gcgtcgttgg tcgattgcgc gtggttcaca tggtcgttgg
     2161 gatggacggg ctgataagcc gccaaacccg taccggcgat ttcgttggcg cggtttgcca
     2221 gcacttcgtt catgttcata ttggactgcg tgccggagcc ggtctgccaa accactaaag
     2281 gaaactgtcc gtcaagcctg ccgttcaaca catcatccgc cgcctgcgta atcaaatccg
     2341 cctgttccgg cttaatcctg ccgagggaaa cattggtcgc ggcggcggct tttttcacca
     2401 gcgccaaagc ataaatcagc ggctgcggca gggtttcgcc gccgattttg aaattgttgc
     2461 ggctgcgctg ggtctgcgcg ccccaatagg cttcggacgg gacttcgaca ttgcccatgg
     2521 tgtcgtgttc ggtacgggtg ctcatgcgtt tctccttttg aaatgtgaat aagagtgatt
     2581 cgcaaatatt ataatggaga ttggaggaat gaggaagtat caaggagaga gggcaaacaa
     2641 atgccgtctg aagccctaat tggtttcaga cggcatttgt ttaggtgtgg ggttacagca
     2701 cggatttcac cgtatcaacc acattgtcca cggtaaagcc gaatgctttg aagagtaaat
     2761 cggcaggggc ggattcgccg aagcggttga tgccgacgac tgcgccgttc agtccgacat
     2821 atttgtacca gccgtcggcg tggccggctt ctacggcgat gcgcggcagg ctttcgggca
     2881 ggacggcggc ttgataggcg gcgtcttggc ggtcgaatac gttggtggac ggcatggaaa
     2941 cgacgcgcac ggcgatgttt tgcgcggcga gggctttttg cgcttccaaa gccagctcga
     3001 cttctgagcc ggtggcaatg atgacggctt gggcgttgcc ttgggcttcg ctgatgatgt
     3061 agccgccgcg tttgatgtcg tccagttgtt gttcgctgcg cgcttggaat ttcaggtttt
     3121 gacggctgaa aatcaggcag gacgggtgat cggcggcttt gacggcttct gcccaagcga
     3181 ccaaggattc ggcggtgtcg cacggccgcc atacgtccat attcggaatc aggcgcaggg
     3241 tggcggtttg ctcgatgggt tggtgggtcg gaccgtcttc gcccaaaccg atggaatcgt
     3301 gggtgaacac aaatacaggg ttgattttca tcagtgccgc catacgcagg gcgttgcgct
     3361 cgtattcgct gaacatcagg aaggtcgcac cgaatggttt gatgccgccg tgcagggcaa
     3421 gaccgttcat aatcgcaccc ataccgaact cgcgcacgcc gtagtggatg tagttgccgc
     3481 ctttgtcgcg ggtaacggag acgctgtttg accagtcggt caggttggac ggggtcaggt
     3541 cggccgaacc gcccaccaat tcaggcaact cttttgccaa gatttcgatg ctgttttggc
     3601 tggctttgcg ggtagcgatg gtttcggctt tggcgcacac ttctttcaat gccgtctgaa
     3661 cgtattcatc gaaattgtcc ggcagctttt tatccatacg gcgcacaaat tctgcggctt
     3721 cggcaggata tttggcttga tattgcgcga acagttcgtt ccagtcggct tccagtttcg
     3781 cgccttgttc tttggcattc cacgcatcgt aaatttcttg cgggatttca aaggcggggt
     3841 aagtccagcc caaatgtttg cgcgtggctt cgatttcgtc cgcgcccaaa ggtgcgccgt
     3901 gggttttgtg gctgccttct ttgttggcac tgcctttgcc gattaaggtt ttgcagcaga
     3961 tgatggacgg tttgccggtt tcggcacgtg cggcttcgat ggcggcttga atggcggcgg
     4021 tgtcatgacc gtttacgtta ggaacgacgt gccagccgta gctttcaaag cgttgcggga
     4081 tgttttcggt aaaccagccg tccactttac catcaataga aatattgtta tcgtcataca
     4141 aaacaatcag tttgcccaag cctaaagtgc cggcgagcga acaggcttcg tgcgatacgc
     4201 cttccatcag acagccgtcg cccatgaaga cgtaggtgta atgatcgacg atgttcaaac
     4261 cgtctttatt aaattcggcg gcaaggattt tttctgccaa tgccataccc accgcgttgg
     4321 caatcccttg ccccaacggg ccggtcgtgg tttccacgcc gtcggtgtag ccgtattcgg
     4381 ggtggccggg ggttttgctg tgcagctggc ggaagttttt caagtcttca atgctcagat
     4441 tgtagccggt caggtgcagc aggctgtaca acagcataga cgcgtggccg ttggagagga
     4501 cgaagcggtc gcggttgtag aatttggggt tggcggggtt gtgattgagg aatttcgtcc
     4561 acaatgtttc cgccatttcc gccataccca taggcgcgcc ggggtggccg gaattggctt
     4621 tttgaacggc atcggccgag aggaagcgga ttgcgtttgc cagttgagac attttgtatt
     4681 ttccttgctg gtgtttcaga taagtggata atcggaaggc gttgattatc gcccgattcg
     4741 cttatgcttt caagaaaagg gcggacgcgg gggaaggcgg cggcagggga ccggtgggga
     4801 aggattttcg atttgcgggc gaagcctgcc attattcctt ttgaaataaa atgttataga
     4861 ttgtgtgccg gattgtcgat agcgtttgtt tatgagcttg cgccgtcggt tctgccgata
     4921 tgggggtgtc ggttttttta gtcttttttt taaccgtatt cggattttgt tcgggcggta
     4981 aggtaaaatc caggcgtttt gatgcggatg ggaaatgtat ccgccccata catccagacg
     5041 gcgcataaag ttgtacaata gcggaaatat attttgggaa aaacggattt tctcaaaact
     5101 tgaaacacaa cgccttaaaa aacaagaaaa atgacgtctg aaaagcaaac ggcggcggta
     5161 acgggcaaac catcaatcaa aaatttaagg aatgcagaat gaccacggaa aaccaagccg
     5221 gcagtccggc atccggaatc ggcacgtccg aacaaacaaa agccgcgccg aaagcaaaaa
     5281 aaacattcga tccgcgagcc agcgtgattc aaatccatcc cgaaggcgaa cgcatccatc
     5341 ccaaaaaggc ggaaggacgg ttcgccaaac tgcgtatcgc cgccgtattg gcgacgcagt
     5401 ttgtgtttta cgtcattccg tggttcaact ggagcggcag gcaggctgtc gttttcaata
     5461 tccccgaacg gcatttcttc attttcggat tgtcgttggg ggtgggcgat ttgatttacc
     5521 ttgccttgct gctgatgatt tgcgccttcg ggctgttttg gtggacgacg attgccgggc
     5581 gactgtggtg cggctattcc tgcccgcaaa cggtttacac cgaaattatg ctgtggatcg
     5641 acaacctggt cgaaggcgat agaaacaaac ggctgaagct ggaaaaatcg ccgtggaatt
     5701 tcactaaaat ccgcatcaaa gccaccaaat acctgttgat tttccttgtc tgcgcgtgga
     5761 cgggcatcac gtttgcaggc tggtttgtcc ctatccgcca gttcgttccc gatttattca
     5821 ctggagcagc aggcggcggc gcgatgtttg ccacagcgtt ttacggcttt atgaccttct
     5881 tcttcgccca cattatgcgc gaaaaagtat gcctgcatat gtgtccgtat gcacgtttcc
     5941 aaagcgcgat gttcgacaag gacacgctga ttgtttctta tgacgcggaa cgcggcgaac
     6001 cgcgcggcgc gcgcaagaaa acggtcaaca aggaagaggc gggtttgggc gactgcatca
     6061 actgtgcgat gtgcgtccaa gtctgccctg tcggcatcga catccgcaac ggtctgcagt
     6121 accaatgtat cggctgcgcc gcctgtatcg acgcgtgtga tgagattatg gacaaaatgg
     6181 gctatccgcg cggattaatc cgttatacga ccgaaagcgc gctggaacac gaatattctg
     6241 aaaaagacat taaaaaacgg ctgctcagac cgcgtgtggc aggttacggc gcggtgttgg
     6301 cgttagttat cattgccttc ctggccggtt tgtccacgcg caaaatggtc gaggtcgata
     6361 ttttgaaaga ccgtggcgta ctggtgcgcg aaaacgccaa aggctggctg gaaaacgcat
     6421 acagcctgcg tatcatcaac aaaagtgaaa aagaacagct cattaccgca agtgtcaaag
     6481 gctttgacga aatcgccctg accgggctgc ccgaaggcgg tatcaaggtt gccccgcgcg
     6541 aaacggtaac ccttcccgtc caagtgtcca ccattccgga atacgcggac aaaggcagcc
     6601 accctatcga atttaccttc caataccgcg aaagcggcgc gcccgacggc aagccggtcg
     6661 tcttggaaga agatgcaacc tttatcggag aataaccgtg tctcaaaaca ctccaatcaa
     6721 accttggtac aaacacgtct ggccgtggat cttgatggcg gggccgattt ttgtcgtcat
     6781 cgccagcgtc gctatgtttt ttgtcgcgca gcagcacgcg acagatttgg ttacggacga
     6841 ttattataaa gacggcaaac atatcgacat ccagcttcat cgggatgaag aagccatcag
     6901 acggcatatc ggggtgcagg ttctcatttc ccccgatatg aatgcggcaa aagtgtttgt
     6961 cggcggcgag tttgacggca aacagccttt gaacctgctg ctgatgcacc cgacccgcaa
     7021 ggcggacgat caaaccgtcg ccctcaagcc cgtcggcagc gcgcagaacg gcagggcgga
     7081 atatgaggcg gtgttcaaaa ccctttcgcc gaccaaccac tggtatgtgc gcgtggagga
     7141 cgcggcagg
//
LOCUS       DAWHRS010000110         7048 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWHRS010000110 DAWHRS010000000
VERSION     DAWHRS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7048
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.110"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            142..576
                     /locus_tag="WIB82_001594"
     CDS             142..576
                     /locus_tag="WIB82_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ3842871.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(624..1967)
                     /locus_tag="WIB82_001595"
     CDS             complement(624..1967)
                     /locus_tag="WIB82_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ3842872.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1972..2625)
                     /locus_tag="WIB82_001596"
     CDS             complement(1972..2625)
                     /locus_tag="WIB82_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ3842873.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2776..3066
                     /gene="gatC"
                     /locus_tag="WIB82_001597"
     CDS             2776..3066
                     /gene="gatC"
                     /locus_tag="WIB82_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ3842874.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            3129..4574
                     /gene="gatA"
                     /locus_tag="WIB82_001598"
     CDS             3129..4574
                     /gene="gatA"
                     /locus_tag="WIB82_001598"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ3842875.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4571..5503
                     /locus_tag="WIB82_001599"
     CDS             4571..5503
                     /locus_tag="WIB82_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ3842876.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDSLKTEARGISDGIQTEKPPRRTA"
     gene            5546..6976
                     /gene="gatB"
                     /locus_tag="WIB82_001600"
     CDS             5546..6976
                     /gene="gatB"
                     /locus_tag="WIB82_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ3842877.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKLTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGEIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
BASE COUNT         1834 a         2119 c         1759 g         1336 t
ORIGIN      
        1 ttttgggaat ctgttttacc ccaatatata aagcaccata ttaaggcgga gtgtcttccc
       61 cactttgacc cgaacccgga aaagacaccg cccaagccaa tcctgatgct gccccgacag
      121 ccaaccatta aggaaatcct aatgaacttt gctttatccg tcattatgtt gaccctcgcc
      181 tctttcctgc ccgtcccgcc tgccggagcc gccgtcttta cttggaagga cggcggcggc
      241 aacagctatt cggatgtacc gaaacagctt catcccgacc aaagccaaat cttaaacctg
      301 cggacgcgcc aaaccaaacc ggcggtcaaa cccgcccaag ccgacgcagg gaagcgcaca
      361 gacggcgcgg cacaggaaaa caatcccgac actgccgaga aaaaccggca gcttgaggaa
      421 gaaaaaaaaa gaattgccga aaccgaacgg cagaacaaag aagaaaactg ccggatttca
      481 aaaatgaacc tgaaggcggt gggaaattca aatgcaaaaa acaaggatga tttgattcgg
      541 aaatacaata acgccgtaaa caaatactgc cgttaatcgg ctctagcgca aacccgatgc
      601 cgtctgaagc ggcacggggt ttgtcatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaat cgctgctggt atggcggttg ataaacaccg atcccgcctg tattttttcg
      781 gcaaaccgcc aagcgcgttc ggtatcggcg gtataaatgc aggcaccgag cccgaacggg
      841 gaatcattgg caagggcgat ggcatgttct tcgttttcgg cgcgcaaaat cagggcggcc
      901 ggcccgaata cttcttctct ccagacgcgg caggcaggat ttaccctgtc taaaaccgtc
      961 gcgggataaa accagcctcg cccttgtggg atttttccgc cggtcaggca taccgcgccg
     1021 tttgaaacgg catcttcaac ctgcccgtga accctgtccc gcaaatcttc gcggtgcagc
     1081 ggtgcaagcg tagtatcggg atgtttgggg tcgcccattt tcaatttagc gcattcggca
     1141 agaaacagcg tgataaaacg atcggctgcg gcttcggtta cgatgatgcg cttggcggcg
     1201 ttacacgatt gccccgcatc gcggaaacgg gaataacagg cttctgcggc ggcacgctcc
     1261 aaatcagcat cgggcatcac gataaaggcg ttgctaccgc cgagctccaa cacggttttc
     1321 ttaaggtttg cgcccgcgtg tgccgcaagg atgcgccccg tatgcgttga accggtaaac
     1381 gccattgcat cggtatcttc aaccgccttg agcgtgcccg cctcatccag ccacacgcct
     1441 gccagaggaa tgccgtctga agccaaatcg aacagtgcct gactgacgcg tgccacgctg
     1501 ggcgcgggtt tgacggcgca cgcgttgccc gcgcacatag cgggaacggc gaaacgcaat
     1561 acctgccaga cgggatagtt ccaaggcatg acggcaaaca ccacgcccaa aggctcgaag
     1621 cgcacctgac tcaaactcgc ctgcgtcgcg atggttttgt gggcaagcag ttcgggggca
     1681 aggcgggcgt aatagcgtat cagttcgata gacttgccga tttccgcacg gcattcgtgc
     1741 aagcagcgtc cgacttcctc acacaccatt tccgcaaaac gctctttctc cgcctccaaa
     1801 cggtcggcaa atttttgcag gcgcgcggca cgttcggtta cgcccagttg cgcgaacgcc
     1861 ccgccgcgca ttttcaaatc cgccagccgc cgttcaaact ccgcataatc ttgagcgggg
     1921 cggcggtaaa gcgtttcgcc cgtaaataca ttgacactgt gaaacatcga atcaacctgc
     1981 cagttgcggg aatatcgttt tcagtcccga cacaataatc tccaccgata ccgccgccag
     2041 catcataccc ataatgcggt ttaaaatcgt cagccccgtc gcgcccagca ggcggctgac
     2101 cttcccggca acgattaaaa tggcataaca aatcgcactg accaccaaac cggccgcgat
     2161 aatcaacgcg atgtcgccgt atgttttagc cgccgaagcg taaataatca cggtcgaaat
     2221 accgcccggg ccgatggtga tcggtatggc gatgggcacg acggcaatcg ctccggcatt
     2281 gcgggcgggg cgcgcctgcc ccgtttccgg ctgcgcgccg agattctgct tggcgggatt
     2341 gtcgttgccg ttcatcatcg aaatggcgat cagcagcacc aaaatcccgc cgccgacctg
     2401 aaacgaaccg acgctgatgc ccaaaacctt cagcagcgta ccgccgatca gcgcaaatac
     2461 cgcaatcacg gcaaacacgg caacggcggc cgtccgcgcg accttcctgc gctccttcgt
     2521 gctgtgcccg ttggtcaggt caaggtaaag cgacaacgcg ctaaacggat taatcagcac
     2581 caaaaaagcc acaatcagct tgccgatttc catgcccaat cccattattt ccccctcctt
     2641 caaacccgtg cggcaggcat ccgatgctgc aaattgccgc cgcaacggat ttttccgtta
     2701 taattaaaaa ttcaagcaat acgccccatc atacccgaac gacggtatct ttaccatcag
     2761 acaaggatgc ttttcatggc actgacactt gccgacgtag acaaaatcgc ccgactctcc
     2821 cgactgcacc tgactgcgga agaaaaagaa aaatcgcttc aagaattaaa cgacattttc
     2881 actatggtcg aacagatgca aaccattaac acagacggca tcgaaccgat ggcgcacccg
     2941 cacgaggccg ccctgcgcct gcgcgaagac gaagtaaccg aaaccgaccg cgccgccgaa
     3001 tatcaggcgg gtgctccgga agtacgcaac cgtctgtaca tcgtaccgca agttatcgaa
     3061 gaataatccg aatatgcttc agacggcatc agcaataccg cccgaagccc tttaaggatg
     3121 gaagatttat gacccaatac acattgaaac aggcaagcgt cctgttgcag tccaaacaga
     3181 tttccgccgt cgaactggca agcgcatacc ttgccgccat cgccgaaaaa aatcccgccc
     3241 tcaacggcta tatcaccatc gaccaagata aaacccttgc agaagcccgt gccgccgacg
     3301 aacgtatcgc gcagggcaac gcctccgcgc ttaccggcgt acccgtcgcc tacaaggata
     3361 ttttctgcca aaccggctgg cgcagcgcgt gcgcttccaa aatgctcgac aacttcatct
     3421 ccccctacac cgccaccgtc gtccaaaacc tgctcgacga aggtatggta acgctcggcc
     3481 gcaccaatat ggatgagttc gctatgggtt cgaccaatga aaactcattc tacggtgcag
     3541 ccaaaaaccc atggaatctt gagcacgtcc ccggcggttc gtcaggcggt tccgccgccg
     3601 tcgttgccgc gcgcctcgcc cctgccgcgc tcggttcgga caccggcggc tctatccgcc
     3661 aacccgcatc gcactgcggc attaccggca tcaaacccac atacggcacg gtttcccgct
     3721 tcggtatggt cgcctacgcc tccagcttcg atcaaaccgg cccgatggcg caaactgccg
     3781 aagactgcgc gattctgtta aacgcgatgg caggtttcga ccccaaagac tccaccagcc
     3841 tcgagcgcga aaaagaagac tacacccgcg atttgaacca accgctcaaa ggtttgaaaa
     3901 tcggcctgcc caaagaatat ttcggcgaag gcaacagcgc cgatgttctg acggcattgc
     3961 aaaacaccat tgatttgctg aaagcccaag gcgcggaatt gattgaagtt tccctgccgc
     4021 aaaccaagct gtccatcccc gcctactacg tcctcgcctc cgcagaagcc agcaccaacc
     4081 tttcacgtta cgacggcgta cgttacggac accgtgccgc ccaattcgcc gatttggaag
     4141 aaatgtacgg caaaacccgc gccgaaggtt tcggcagcga agtcaaacgc cgcatcatga
     4201 tcggcactta tgtactgtcg cacggctact acgatgccta ctatctcaaa gcccaaaaac
     4261 tgcgccgcct cgttgccgat gattttcaga cggcatttgc acggtgcgac ctcatcctcg
     4321 cgccgaccgc acccactgca gccccaaaaa tcggagcgga tgcttcgccg gttgaaacct
     4381 acttgagcga tatctacacc atcgccgtca acctcgccgg actgcccgca ttgaccctgc
     4441 ccgcaggctt cagcggcggc ggactgcccg tcggcgttca gcttgtcggc aactacttcg
     4501 ccgaagccaa aatcctcggt gcggcgcatc aaatccaact caacagcgat tggcacggca
     4561 aacgacccga atgaagcaga accgcacctt taccttcccc gattttcgca ccgtttacag
     4621 ctatgcgcct ttatatcggc tgcaacattt aaaatacaca ttgcgaaaat ttttcggaaa
     4681 aaaagaaatt tacgccttcg agcagtttgt caacgcctcc cctatccgtc aggggctgtt
     4741 cctccactgc ccgcaaaatg cctatccgct gctgcgcgaa tttgttgaca ggcgttttaa
     4801 ctgcaaacgc cgtttagatg cgatgacggc agattttctc atggcggaaa aactgttcgg
     4861 cacagacatc ctgcaccaaa tggaagacta ccgcttccat ttggtcttgg cgcacctttc
     4921 agacggcatc agcttgtggc tcaaccgcaa cgacaactgc gtcgaagaag gcgcgtggtc
     4981 tttatctttg cgcgacgaag caggcaaccg gctgtatatg gcgactttcg cctttgtcgg
     5041 cacacacctg ctgacagcct ccgtacaagg gccggcgggt gaagaagcca aagacaccgt
     5101 ccgccgcata accaaacaac tccacggctt gcgtccccaa caactgatgg taaccgccct
     5161 gcaatatttc gccgccgtac tcggcttgga cggcgcaatg ggcattgcac aaaaacatca
     5221 ggtcaaactg cgctggaaac ttaaaaagcg cgtcaaaatg aattacgacg cattctggca
     5281 ggaatacggc gcaagtttgg aacgggacgg ctactggcat ctcccccaaa cccccgcccg
     5341 caaagacctt gccgacatcg aaagcaaaaa gcgttcgatg taccgcaagc gttatgaaat
     5401 gctggacaat atggttgcag agatgaaaga cagtctgaaa acagaagcac gcggcatttc
     5461 agacggcatc caaacggaaa aaccgccccg ccggacagcc tgacgcgaag actatcgaat
     5521 tgatatttta gagaaagaag ctcttatgac ctgggaaacc gtaatcggct tggaaatcca
     5581 cgtccaattg aacaccaaat ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga
     5641 acccaacgcg cacgccagcg tagtggaatg cgcgctgccg ggcgttttgc ctgtgatgaa
     5701 ccgtgaagtc gttgaaaaag ccatcaaatt gggtttggct ttagatgcga aaatcaatca
     5761 gaaaaacgtg ttcgaccgca aaaactactt ctatcccgac ttaccaaaag gttatcaaat
     5821 cagccagttg gacttaccga ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga
     5881 tgtgaaaacc atcaacgtaa cccgtgcgca catggaagaa gacgcaggca agtccgtgca
     5941 tgaaggcttg aacggcgcaa ccggtatcga cctgaaccgc gccggcacgc cgctgttgga
     6001 agtggtatcc gaacctgaaa tgcgttccgc cgccgaagcc gttgcctacg ccaaggcctt
     6061 gcacagcttg gtaacctggc tggacatttg cgacggcaat atggcggaag gctcgttccg
     6121 cgtcgatgcc aacgtatccg tgcgcccgaa aggtcaagaa gagttcggca cgcgccgcga
     6181 gattaaaaac ctcaattcct tccgtttctt ggagcaggcg attaattacg aagcggaagc
     6241 gcaaatcgag attttggaag acggcggcaa agtacagcag gcaaccatgc tgtttgatcc
     6301 cgaaaaaggc gaaacccgcg taatgcgcct gaaagaagat gcgcacgact accgctactt
     6361 ccccgaccct gatttgctgc ccgttatcat ttcagacgcc caaatgcaaa aagccaaagc
     6421 agaaatgccc gagctgccga aagaaatggc agcgcgtttc gtggcggatt acggcgtgtc
     6481 cgaatacgac gcgcgcctgc tgaccgcaag ccgtgcgcag gctgcctatt ttgaagaagc
     6541 cgccaaagaa agcggacaag gcaagctgac tgccaactgg atgaacggcg aacttgccgc
     6601 cgcgctgaac aaagaaggca tggaacttgc cgacagcccg attaccgccc cgcgcctcgc
     6661 cgcgctggtt ggcaaaatcg ccgacggcac attaagcagc aagttagcga aaaaagcctt
     6721 tgaagccatg tgggcagaac ccgaagccac cattgccgaa atcattgaaa aacacggttt
     6781 gcaacagatg accgacaccg gcgagattga agccatggtg gacgaagtgc tggcaaacaa
     6841 cgccaaagcc gtggaacagt ttaaatccgg caacgaaaaa gccctgaatg cgattgtggg
     6901 acaagtgatg aaggccagca aaggcaaagc caaccccgcg caggttcaag agctgattaa
     6961 agccaaactg gcttaatccg ttatcacaca ggtcgtctga aagcaaagtt ccaacgaagg
     7021 taaaacagga aataagcttt cagacggc
//
LOCUS       DAWHRS010000111         7037 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWHRS010000111 DAWHRS010000000
VERSION     DAWHRS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.111"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..222)
                     /locus_tag="WIB82_001601"
     CDS             complement(<1..222)
                     /locus_tag="WIB82_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3842878.1"
                     /translation="MASSGVNNEIKDKKLSLWAKRQDGSVKWFCGQPVTRADTDTDTA
                     VTAASDKQIDPKHLPSTCRDESTAGCTNHP"
     gene            complement(251..580)
                     /locus_tag="WIB82_001602"
     CDS             complement(251..580)
                     /locus_tag="WIB82_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3842879.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(658..876)
                     /locus_tag="WIB82_001603"
     CDS             complement(658..876)
                     /locus_tag="WIB82_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ3842880.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            998..1951
                     /locus_tag="WIB82_001604"
     CDS             998..1951
                     /locus_tag="WIB82_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ3842881.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIIITNKVMISADIIAHAPQLELIAVSATGVNNVDIEAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLARYAQAFGMRVVFAEHKHAPVVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALVAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2026..4083)
                     /gene="metG"
                     /locus_tag="WIB82_001605"
     CDS             complement(2026..4083)
                     /gene="metG"
                     /locus_tag="WIB82_001605"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ3842882.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLDECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPILPKVAEN
                     AAKFLNLEAITWANTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAVPAA
                     EESQYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYLNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(4198..6036)
                     /gene="glmS"
                     /locus_tag="WIB82_001606"
     CDS             complement(4198..6036)
                     /gene="glmS"
                     /locus_tag="WIB82_001606"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ3842883.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GSFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            6318..6848
                     /locus_tag="WIB82_001607"
     CDS             6318..6848
                     /locus_tag="WIB82_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842884.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTVQAYRDARNALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPGHCCFSGG"
BASE COUNT         1474 a         1680 c         2113 g         1770 t
ORIGIN      
        1 cgggtggttt gtgcaaccgg cagttgattc atcgcggcag gttgacggca ggtgcttggg
       61 gtcgatctgc ttgtcgctgg cggcggtaac ggcggtgtcg gtgtcggtgt cggcgcgcgt
      121 aaccggctgt ccgcagaacc attttaccga accgtcttga cgcttggccc acagggagag
      181 ttttttgtct ttgatttcgt tgtttacgcc gcttgaagcc attatgtcag acggtattgc
      241 ccgggcagct ttattcgtac actttcagca gctcgacttc aaatatcaaa gtggcgtgcg
      301 ggggaatcac gccgcccgcg ccgtgtgcgc cgtagcccat ttccgaaggg atggtcagct
      361 tgcgtttgcc gccttccttc atgccgccga agccttcgtc ccagcctttg atgacttgtc
      421 cgacaccgag cgtgatggtc agcggctggc ggcggtcgag gctggagtcg aatttggttc
      481 cgttttccag ccaacctgtg taatgcacgg taatctcttt gcctttaact gcttcttttc
      541 cgaagccttc ttgcaagtct tcaataatca ggccgcccat atttgtcctt tcgttgcttg
      601 ttggtcaaaa cggcaagggt aacataccgt ccgtcgaagt caaatgccgc tcaaacgtca
      661 gctgcatcgg tgcagctgaa acggctgtgt ttgttggact gttttatttt tttcgtaaag
      721 gttccatgct ttttcatgga aatagaaaac gacggtgttg attaggggct cgaccagcgc
      781 gaccgccccc gatacgccta tactgcccgt cagtacatag gttacactga aggcggtgct
      841 gaaatgcagt gcggcaaaag tcagggtttt aagcatcatc ctctcccgaa ttggacattg
      901 acggaggaat gataaaggtt atcataaggt tgcgcggttt aaatttgcta tttgttgtta
      961 gtgtaaataa atcatttttc aaatggggat agaaattatg aatcataaaa agatcgttgt
     1021 tttggatgcg gatactttac ccggccgggt ttttcatttt gattttccgc acgagcttgc
     1081 ggtttacggt acgacaggtg cggatgaaac ggcagaacgg gtgcgcgacg cgcatattat
     1141 catcaccaat aaagtgatga tttcagcgga tatcattgca cacgctccgc aacttgaatt
     1201 gattgccgtc agtgcgaccg gtgtgaacaa tgtcgatatt gaggcggcga aggcggcggg
     1261 cgttgcggta tgcaatgtcc gcgcctacgg aaacgaatcg gttgcggaac acgcctttat
     1321 gctgatgatt gcgttaatgc ggaatttgcc cgcctatcag cgtgatgttg cggcaggatt
     1381 atgggaaaag tcgccgtttt tctgccatta tggcgcgccg attcgggatt tgaacggtaa
     1441 aacgctggcg gttttcggac gcggcaatat aggacggacg cttgcccgat atgcacaggc
     1501 attcggtatg agggtagtgt ttgccgaaca caaacacgcg cccgttgtgc gtgaaggcta
     1561 tgtttccttt gaagatgcgg tacagactgc cgacgtgttg tcgctgcact gtccgctgaa
     1621 cgcccaaact gaaaatatga taggcgaaaa cgaattacag cagatgaagc ccggtgcggt
     1681 tttgatcaat tgcgggcgcg gtgggctggt ggatgaaaat gcgctggttg ccgcactcaa
     1741 atacgggcag attggcgggg caggtgtcga tgttttgacg gaggaaccgc ctaggggcgg
     1801 caatcccttg ctgaatgcac gacttcccaa tctgattgtt acgccgcata ccgcgtgggc
     1861 aagtcgtgaa gcgttggaca ggctgttcga tatattgttg gcgaacattc acgcctttgt
     1921 gaaaggcgag gcgcaaaacc gcgtggtttg aacctgtcgg gattgcggaa aaaaatgccg
     1981 tctgaacgcc tcaagggttc agacggcatt ttttgagatt tctgcttaac cgactttgtc
     2041 gcccggttgc gcgcctgcat ccacatccaa gagcttcagt ttcccgtctg ccgtggcggc
     2101 agagagaatc atgccttcgg atacgccgaa ttttgccatt ttgcgcgggg cgaagttggc
     2161 gacggcgatg accatccttc cgtttaactc ggcagggttc aggtaagacg cggcgatgcc
     2221 ggagaagatg atgcgttttt caaaaccgaa atcgaggtcg aatttcaaaa gtttggtgct
     2281 gccttcgaca gcttcgcagt tcaatacttt ggcaacgcgc atatcgattt tcataaagtc
     2341 gtcgaagctt gcctgttcgg caactttttc gtattgactt tcttcggcgg caggcacggc
     2401 tgcggcggcg atgctttgtt tgttggcttc gattaaatcg tccacttgtt tttgctccac
     2461 tcgttgcatg agatgttcgt atttgttgat ggcgtgttcg cccaaggttt cgcgtgtgtt
     2521 tgcccaagtg atggcttcca aattcaggaa tttggcggcg ttttcggcca ctttcggcaa
     2581 gatgggggca aggtaggcgg tcagcatggt aaaggcgttg atgagttcgc tgcatacttc
     2641 gtgcaggcgt tcgtcttggc cttcttgttt cgccagttcc caaggtttgt tggcatcgac
     2701 gtattcgttg acaatgtctg ccaaggccat gatgtcgcgc agggcgcggg cgtattcgcg
     2761 gttttcgtat tgctcggcga tggtgtcgct ttcggctgca agttttgcca gcaatgcgct
     2821 gcctgaaacg tctttcagac ggccttcaaa gcgtttggcg atgaaacctg atgcgcgggc
     2881 ggcgatgttg acgtatttgc cgacgaggtc gctgtttacg cgcgaaataa agtcttgcag
     2941 gttcaaatcg atgtcttcga ttttgctgtt gagttttgcg gcgatgtagt agcgcatcca
     3001 ctcggggttc aggccttgtt ccaaataaga tttggcagtg atgaaggtgc cgcgcgattt
     3061 ggacattttt tgtccgtcta cggtcaaaaa gccgtgtgcg tacacgccgg tcggggcgcg
     3121 gtggccggag aaatgcagca tggcaggcca gaacagggcg tggaaataga ggatgtcttt
     3181 gccgatgaag tggtacatct cggtttggct gtcggctttg aagtattcgt caaaatcgac
     3241 gccgatgcgg tcgcacaggt ttttaaacga cgccatgtag ccgacgggcg cgtccagcca
     3301 gacgtagaag tatttgcccg gcgcgtcggg gatttcaaaa ccgaaatacg gtgcgtcgcg
     3361 ggaaatgtcc cagtcggaca gggtggtttc ttcgccttcg cccagccatt ctttcatttt
     3421 gttgagggct tcgggttgca gatggggctt gccgtcgtgc ggattgttgc cggaagtcca
     3481 tgctttgagg aagtcggcgc attcgtccag tttgaagaag aagtgttcgg attcgcgcaa
     3541 ttcgggtttg gcaccggaaa cggcggaata cggattaatc agttcggtcg gggaataggt
     3601 cgtgccgcag acttcgcagt tgtcgccgta ttggtcttgg gcgtggcatt tcgggcattc
     3661 gcctttaacg aaacggtcgg gcaggaacat ttgtttttca gggtcgaaaa gctgctcgat
     3721 gacgcggctt tcgattttgc cgttggcttt cagcgcgcgg taaatgtctt gggaaaactg
     3781 tttgttttca ggggaatggg tgctgtaata attgtcgtag ccgatgttga aaccggtaaa
     3841 gtcggcgagg tgctcttcgc gcactttggc aatcatgtct tcgggcgcga tgccttgttt
     3901 ttgcgcggca agcatcacgg gcgtgccgtg ggtgtcgtcg gcgcagcagt agtggcattc
     3961 gtggccgcgc agtttttgga agcgcaccca aacgtcggtt tggatgtgtt cgaccatgtg
     4021 gccgaggtgg atgctgccgt tggcataggg cagggcggag gtaactaaga ttttgcgtgt
     4081 catattgtgc tttgcaaaca atgggtaaag gcggattata ccgcaaatca aacggggaaa
     4141 tgccgtctga agcctgaaaa atcgggcttc agacggcatt ttgccaatcg gcgggattta
     4201 ttcgacggtt acggattttg ccagatttcg cggtttgtcc acatccgtgc ctcgggcgag
     4261 ggcggtgtgg taggcgagaa gctgcacggg gatagtatgc acgacggggg acagttcgcc
     4321 gacatggcgc ggcgcacgga taacgtgcac gccttcggtg gcattaaaat tgctgtcgag
     4381 gtcggcaaag acgaaaagtt cgccgccgcg cgcgccgact tcctgcatat tggctttgac
     4441 tttgtccaac aggctgtcgt tgggcgcgat gacgacgacg ggcatatttt cgtccaccag
     4501 ggcaagcggc ccgtgtttca gctcgccggc aggataggct tcggcgtgga tgtaggtgat
     4561 ttccttcagc ttcaacgcac cctcgagggc aatcgggtaa tggatgccgc gccctaaaaa
     4621 cagcgcgctg gttttcttgg caaactgttg cgcccaggcg gcaatttgcg gttcgaggtt
     4681 caaagcgtgc tgcacgctgc cgggaagctg gcggagttct tcggtataac gtgcttcatc
     4741 ttcgccggat accaagccgc gcactttcgc cagcgttacc gccaaaccga acagcgcgac
     4801 cagctgcgtg gtaaatgcct tggtcgaggc gacgccgatt tccgcaccgg cacgggtata
     4861 aagcacgagg ctgctttcgc gcggcagggc ggattccatc acgttgcaaa tggagaggct
     4921 gtggcggtgt cccaaagatt tggcgtattt caaagcctcc atcgtgtcca gcgtttcgcc
     4981 ggattgggaa atggtaatga ccagttggtc gggatcggca atcacgctgc ggtatcggta
     5041 ttcgctggcg atttcgacgt cagccgggat tttggcgatg gattcgagcc agtattttgc
     5101 ggtcagcgcg gcgtaatagg acgtgccgca ggcaaggatt ttgacgctgc ggatgctttc
     5161 aaacacgctt ttggcgtttt tgccgaagtt ttcggggatg aagctgccgt cgaggaaaac
     5221 ttccgccgta tccgcaatcg cgcgcggctg ctcgtggatt tccttttgca taaagtggct
     5281 gtacggtccc agttccaaag aggcgagcga gagttcggat accttgactt tgcgttcggc
     5341 aggcaggccg tttttatcgg tcagccgttt gatgccgtct gaagccagca gcgcgatgtc
     5401 gccgtcttca aggtacgcca cgcggcgcgt aaaggcgatg acggcggata cgtccgaagc
     5461 gataaaggtt tcatcgtcgc ccaaagcgac caaaagcggg cagcccatac gcgccacaac
     5521 taattcatca ggcttgtctt gggcaataac cgcgatggcg tatgcgccgt ggaaacgttt
     5581 gaccgcttcc tgcacggctt caaacagcct gccgccgttt tgcgcgtatt cgtgattgat
     5641 gctgtgtgcg atgacttcgg tatccgtttg cgattcaaaa cggtatccca aaccttccaa
     5701 gcgtttgcgt tcgctttcaa agttttcgat gatgccgttg tggacgaccg caatcatccc
     5761 gccgctgatg tgcgggtggg cgttcggctc ggtaacgccg ccgtgtgtcg cccagcgcgt
     5821 atgcccgatg ccgatgccgc cgctgatgcc tttttcgcgt gccgcgtctt ccataagctg
     5881 cacgcgtccg acgcggcgca cgcgtttgat tttgccgtcg gtgttgacgg caatgcctga
     5941 tgagtcataa ccccggtatt cgaggcgttt gaggccgtcg gtcagaaaat cgacgacgtt
     6001 gtgatgggcg cggatggcgc cgacgatacc gcacataact gttccttagt atccggttga
     6061 aaaaaaacag gcgcggacgg ctcccgtgcc gcaccttcct cttcggatta taaaccgcct
     6121 cccgcgccga aaaacagcaa aatgccgtct gaaggcttgg gcttgctcaa aaaagggagg
     6181 gatttccccg cctgaccggg atgggcgttc agacggcatt gcctgccgcc ggtttatagt
     6241 ttttacaaat caatattgac aagctgaaaa aaaaaaacaa tatactcgct cggtcttaat
     6301 gttaacggag tatagaaatg aaacaaatgc ttttagccgt cggcgtggtg gcggtgttgg
     6361 cgggctgcgg caaggatgcc ggcggttacg agggttattg gcgcgaaaag tcggacaaaa
     6421 aagagggcgt gattgccgtc aaaaaagaaa aaggcaatta cttccttaat aaaatcaacg
     6481 tatttacagg caaggaggag tctttgcttt tgtctgaaaa agacggcgcg ctttcgataa
     6541 acacagggat aggggaaatc ccgatcaaac tttccgacga cgggaaagag ctgtatgtcg
     6601 aacgcaggca gtatgtgaaa accgatgcgg cgatgaagga caaaatcatc gcccaccaga
     6661 aaaagtgcgg acaaacagta caggcatacc gcgacgcgcg aaatgcgttg ccgtcaaacc
     6721 aaacgtatca gcagcgtcag gcggcgatcg agcaattgaa acggcggttt gaagccgagt
     6781 tcgatgaatt ggaaaaagaa atcaaatgca acggcagacc cgggcattgt tgctttagcg
     6841 gaggataacc gggaggatgc cgccgtccga atcggacgtg cggtttccgt accggtacag
     6901 gcggacggga atgtccgcct ttttttgttc ggacgcgttt gaatatccgt ccgattccgt
     6961 ccgtttgcaa agggtatttt tgttcgggcg gaaattatag tggattaaca aaaaccagta
     7021 cggcgttgcc tcgcctt
//
LOCUS       DAWHRS010000112         7035 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWHRS010000112 DAWHRS010000000
VERSION     DAWHRS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.112"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..435)
                     /locus_tag="WIB82_001608"
     CDS             complement(<1..435)
                     /locus_tag="WIB82_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3842885.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRSALLDVYGEADGKPFFLPLNLLLGFMG"
     gene            686..3136
                     /gene="lon"
                     /locus_tag="WIB82_001609"
     CDS             686..3136
                     /gene="lon"
                     /locus_tag="WIB82_001609"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ3842886.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNILTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            3318..3587
                     /locus_tag="WIB82_001610"
     CDS             3318..3587
                     /locus_tag="WIB82_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ3842887.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(3646..4080)
                     /locus_tag="WIB82_001611"
     CDS             complement(3646..4080)
                     /locus_tag="WIB82_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842888.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(4073..5380)
                     /locus_tag="WIB82_001612"
     CDS             complement(4073..5380)
                     /locus_tag="WIB82_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ3842889.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            5553..5948
                     /locus_tag="WIB82_001613"
     CDS             5553..5948
                     /locus_tag="WIB82_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ3842890.1"
                     /translation="MMRQRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDVKPVDYPEILIIGTGAAQEFIHPKIIADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            6025..>7035
                     /locus_tag="WIB82_001614"
     CDS             6025..>7035
                     /locus_tag="WIB82_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3842891.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LES"
BASE COUNT         1763 a         1819 c         1859 g         1594 t
ORIGIN      
        1 acccataaac cccagcagca gattgagcgg caggaaaaac ggtttgccgt ccgcctctcc
       61 gtacacgtcg agcaaagcgg agcgtatatc gggcgcgcgt ttgttgtcca acacaaaacc
      121 ggggcggatt tcaacggcaa gcatattcag aatacggctc tgctgcgcct gcttcatacg
      181 gtgggcgtgg aaaagggcgg cgatatccgc atcggaacgg acggcggcag gtttgcggac
      241 cttcttcttc attgcggaaa gcacctgttt ggcattgtgg aaatcgccct gccagacagt
      301 tgcaatattt tgataggcag ccttcaaaat gccgtttgca ccactttcgc ggacataatg
      361 ccaacctttg ggcggctttt gcagactttc gttgcgccat tcgaacccgt tatcgggaaa
      421 aataaaagaa gacatggaat acctgcgtca tgttttgaaa atagggcggc agaaccgcaa
      481 accatacgga tggtacagca aggagcggca acacagaaca gttttttgtt cccgccttgt
      541 ctttccaagc ccatgccgtc tgaagccgga atgtttcaga cggcatcgca tcaaactcca
      601 taaataaacc acatatgctt gaaataatac cttcaacccc aatgtacgcg aaaatcggca
      661 atctgtcaga cacaagagag tacctatgac acaaaaagaa aagcattttg aggaatatgc
      721 cgccttggca acccttcctt tgcgggatgt cgtcgtttac ccgcatatgg ttctgccgct
      781 gtttgtcggc aggccgaaat ccatcgccgc actggaaaac gccattaccc gcgaggagcc
      841 ggttttcctg ttggcgcaaa ccgatgcggc gctagaagaa ccggttgccg ccgacctgta
      901 tcagaccggt acggtcgcac aagtcctgca agtgttgaaa ctacccgacg gcacggtaaa
      961 agtattggtc gaagggctgt atcgcggacg tgttctgacc attgaagaca cgggcggtct
     1021 gttcgtttcc catatagaga cggtcgtgga agaagacacg ggcggcaata ccgacctcga
     1081 agccgtgcgc cgcaccctgt tggcgcagtt tgaacaatac gccaaactca ataaaaaaat
     1141 ccccgccgaa attatcggca gcattaacgg cattgccgaa aacagccggc taaccgatac
     1201 ggtcgcagcg catttgcagt tgaaactggc gcaacgccaa cagattttgg aaattcccga
     1261 aatcggcaaa cggatggaat tcctgctggc acagctggaa tccgaactcg acattatgca
     1321 ggccgaaaaa cgcatacgcg gacgcgtcaa acgccaaatg gaaaaatccc agcgcgaata
     1381 ttatctgaac gaacagatta aagcgataca caaagaattg ggcgaagaag acgaaaacgg
     1441 cgaactggat gccttggaag caggcatcaa aaaggcgggg atgagcaaag aagcggaaga
     1501 aaaatgcctg tccgaactga aaaaactcaa aatgatgccg ccgatgtctg ccgaatccac
     1561 cgtcgtacgc aactacatcg acaccctgct cgaactgccg tggaagaaaa aatcccgcgt
     1621 cagcaaagac atcgccaaag ccggattggt gctggatacc gaccactacg gcctggaaaa
     1681 agtcaaagaa cggattttgg aatacctcgc cgtccaaaaa cgtatggaca aactcaaagg
     1741 cccgattctg tgcctggtcg gccctccagg cgtgggcaaa acctctttgg gcgaatccat
     1801 cgccaaagca acggggcgga aatatgtccg catggctttg ggcggcgtgc gcgacgaaag
     1861 cgaaatccgg ggacaccgcc gcacctatat cggctccatg cccggtaaga ttttgcagaa
     1921 tatggcaaaa gccggcgtaa aaaacccatt gttcctgctc gacgaaatcg acaaattggg
     1981 tagcgacttc cggggcgatc ccgccagcgc gttgctcgaa gtgcttgatc ccgagcaaaa
     2041 caacaagttc gccgatcatt atgccgaagt ggattacgat ttgagcgatg tgatgtttat
     2101 tgcgacttca aacagcctga atatcctgac accgctgctc gaccgtatgg aaatcatccg
     2161 tctgtccggc tataccgaag acgaaaaaat caatatcgcg atgcagtacc tcgtgccgaa
     2221 gcaaatgaag cgcaacggcg taaaagaagg ggaattggca atcgaagaaa gcgcggtgcg
     2281 cgatattatc cgttactaca cccgcgaggc gggcgtgcgt tcgctcgacc gcgagattgc
     2341 caaaatctgc cgcaaggtgg tgatgcagat taccttggac gaagactcaa aaaccaaaaa
     2401 aaccggaaaa gccaagccca gggcggttaa agtaaatgag aaaaatctgc acgactattt
     2461 gggtgtgcgc cgcttcgatt acggcgttgc cgaaagcgaa aaccgtatcg gacaagtaac
     2521 cggtttggcg tggacggaag tcggcggcga attgctgacc gtcgaagccg cagcattgcc
     2581 gggtaagggc gtgattcagt gcaccggcca gttgggcgat gtgatgaagg aatccgtgtc
     2641 cgcagcgtgg tcggttgtcc gctcccgcgc ggaatcagtg ggcttggctc ctgattttta
     2701 cgagaaaaaa gacatccatg tccatgttcc cgaaggtgcg acgccgaaag acggtccgag
     2761 tgcgggcatt gccatgactt tggcgatggt gtctgcattc accaaaatcc cggtgcgcgc
     2821 cgatgttgcc atgacgggcg aaattaccct gcgcggcgaa gtcctgccga ttggcggtct
     2881 gaaggaaaaa ctgttggctg cgcttcgcgg cggcatcaaa cacgttttaa ttccaaaaga
     2941 caacctcaaa gacttggaag aaatccctga aaacgtgaaa accggcctga ccatccatcc
     3001 ggtcaaatgg atagacgagg tattggctct gggtttggaa agccagcctg agccttgggc
     3061 agaaccttct ggtgcggaag cggcggcgga atccgcttca aaaccaaaac cccgcagcag
     3121 ggcaaccaaa cattgaaacg caggaaatgt gttgtaaaaa tgcggtttcg tcctgaaagc
     3181 ctgtcaaata gggtgattcc gtattttttg cttgacacgg caatttcaga attgctataa
     3241 agcgaaagtt gctcaagcag tacaaacccc gagtattcgg gcatttcatc attttttaag
     3301 acaggaaggg actgattgtg aacaagtctg aattgatcga agcgattgct caagaagccg
     3361 acatttccaa agccgccgca caaaaagctt tggatgccac taccaatgca gtaaccaccg
     3421 ccctgaaaca aggcgacacc gttactttgg tcggtttcgg tactttctac gtgggcgaac
     3481 gtgcggaacg ccaaggccgc aaccccaaaa ccggcgagcc tctgaccatt gccgccgcca
     3541 aaacgcctaa attccgtgca ggtaaagcat tgaaagatgc actgtaagcc gttttttatg
     3601 aaaaaagccg attctttaaa gaatcggctt ttttatcggt ccacattatt ctgatttcaa
     3661 atcggcaaca cactgcttgt cacgtgcttc aaaggcattt gcgccgccga gcaggtcaag
     3721 ctgttcttgt gcgccgagtt tgccgaagga tctaatctgt ttctcgctca atctgtccaa
     3781 aggctgctcc cacatacatt tgcagtagtc gacggcgagg cgggtattgt ttgaatctaa
     3841 accgcgggcc cgcaaatcgt tttgccattt ttcggcaaac ggaatattct tcacgcaaga
     3901 ctcgacaatt ttctgttttg cctgcggttt ggacatcgcg cattgggaga gcagggcggt
     3961 taaagcgagc aacgccaaaa tgacccacgc ccaaatgcgg atggtgcgga ttttggcttt
     4021 tgcttttttg cgcgcggcaa cctgctcttc cgtcggcatt tcgtgtttcg gctcagtcat
     4081 gcaggctttc catgcggatc atggtaatcg gtttttccac gcaatccagt gcttcgatgg
     4141 ctgcgattgc cgacttgatg tgtttttcga ccgtgctgtg ggtcagaatc acgatttcgg
     4201 cagtggtctg atcaatcacg cctttttgaa tcagtgcttc gatggacacg ttttcttgtg
     4261 ccaacagcgc ggcgatttgc cccagcgtgc ccggttcgtc tttggcttgg acgcgcaggt
     4321 agtagctgct ggtaatttcg tccataggca ggatggtttg cgcttggact tgcgcgggtt
     4381 ggaacgccag atgcggtacg cggtgggcgg tatcggcttc aaccaggcgg gcgatgtcga
     4441 tgatatcggc aaccacggcg gaagcggtcg gcaatgcgcc cgcgcccgcg ccgtaatata
     4501 aggtttcgcc aaccatatcg gcgttgacgc gcacggcgtt catcacgccg ttgacgtttg
     4561 ccaagaggcg gctttcggga atcagggtag ggtggacgcg cagctcgatg cctttgccgg
     4621 ttttgcgggt aatgcccaac agtttgatgc gatagccaag ttcttcggcg tatttgatgt
     4681 cgcggctgtc gagtttgctg atgccttcga ggtagcaggc ggaaaagttc atcggcgtgc
     4741 cgaatgccag tgcgctcatg atggtgattt tatggcccgc atcgttgcct tcgatgtcga
     4801 aggtcggatc ggcttcggca taacccaatg cctgcgcttc tttcagtaca tcggcaaacg
     4861 cgctgccttt ttcgcgcatt tcggagagga tgaagttgct ggtgccgtta ataatgccgg
     4921 cgatggattt aatcctgttt gccgccaaac cttcgcgcag ggctttgatg attgggatac
     4981 cgcccgctac tgccgcttca aattggacga tgacgttttg tttttccgcc agcgggaaga
     5041 tttcgttgcc gtattcggcg agcagttttt tgttggcggt aacgatgtgt ttgccgtttt
     5101 caatggcttt caacaccgca tctttggcaa tgccggtacc gccgaacaat tcgacgacga
     5161 catcgacgtc ttcacgtgcg accagttcga acggatcttt gacaaaggct gcggacgggc
     5221 aggtttgtcg ggctttttct tcactcaaat cgcacacggc agaaatacgg atttcgcgcc
     5281 ccaagcgacg ggaaatttcc tccgcgttgt cccgcaacac ggcagccgta ccgccgccga
     5341 ccgtacccaa acctaaaaga ccgatgttta ctggcttcat tgtgtctcct tgtaagccga
     5401 ctgaaatgta aatattgaaa gacgaaaata tccgctgccg atataattgt gccgcacttt
     5461 gaatcaaatg ccgtctgaaa tcggcaggcg ggtcagatga aatctgccaa tcctacatga
     5521 atttgtctga ttttgcatcc ctttcggtgt agatgatgcg gcaacggggt aaaaaaatgt
     5581 tgtttgaaga aaatccgata gacggacagt ttgcggaata tgaatgcggt gcgggcggaa
     5641 tccggctggc ggggcaaagt ttccataaac ccgtgcttgt acataaggat tcggtctgcc
     5701 tgtcgcaatg ccgaaccttg tccgatctga ctccggaaaa cctgttgtcc gacgtcaaac
     5761 ctgttgacta tccggaaata ttgattatcg ggacgggcgc ggctcaggag tttatccatc
     5821 ccaaaatcat agcggatttt tcccgaatcg gaatcagcgt ggaatgcatg aataccgatt
     5881 cggcattcag gacattggtt ttcctgcact cggaagggcg cagggcttgg gcttggcttc
     5941 agccgtaaat ttcccgtttc agacggcatc ggcactgact ttcaggtaaa atacgggctt
     6001 ttcccgcccg acgatgtttc cgttatgatt gaaatcaaaa acctcaccct gcaacgcggt
     6061 ttgaaagtcc tgctcgacaa agccaacgct accgtcaatc ccggtcagcg cgtcggtttg
     6121 atcggcaaaa acggaacggg caaatcgagc ctgtttgcct taatcaaggg tgaaatcact
     6181 caggacggcg gcgatgtctc gattccgaaa aactggcggc tcgcttccgt ttcccaagaa
     6241 acgcccgatt tggatatttc cgctttggat tacgttttgc agggcgatgc cgagttgcag
     6301 gcttttcaga cggcattgag gcaggcagaa gcgcaaaatg acggcatgaa gcaggcggaa
     6361 tatcatgcta aattggaaga aatcgacgct tataccgcgc cggcgcgtgc ggcaaaattg
     6421 ttgaacgggc tgggtttttc gcaagaagaa cacagccgcc ccgtcaaatc cttttccggc
     6481 ggctggcgta tgcgcctgaa tcttgcgcaa gccctgattt gccgcgccga tttgctcttg
     6541 cttgacgaac cgaccaacca cttggatttg gaaaccgtct tgtggctgga aaaccacctt
     6601 gcttctttac cctgcacgca aatcatcatt tcccatgacc gcgattttct caacgcggca
     6661 actacccaaa ccattgaatt atcgcagcaa aaactcacgc aatacggcgg caattacgat
     6721 ttttaccaaa acgaacgtgc gcagcgtctc gcgcaacaac aagctgccta tgtcaaacag
     6781 caggcgcaaa tcaaacattt gcaatccttt atcgaccgct tcaaagccaa agccaccaaa
     6841 gccgttcaag cgcaaagccg catgaaggct ttggcgaagc tcgaacgcat cgctcccgcg
     6901 catctggaca gcgagttttc ctttgagttt taccatcccg accatctgcc caatcctttg
     6961 ttaaagctag aacacgctga tttgggttac gaaggcaaaa ctgtcctgca cgacattacc
     7021 ctgtcgctgg aaagc
//
LOCUS       DAWHRS010000113         6977 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWHRS010000113 DAWHRS010000000
VERSION     DAWHRS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.113"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(91..1887)
                     /locus_tag="WIB82_001615"
     CDS             complement(91..1887)
                     /locus_tag="WIB82_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ3842892.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLSEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVCLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPSAEQKSDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2084..2284
                     /locus_tag="WIB82_001616"
     CDS             2084..2284
                     /locus_tag="WIB82_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842893.1"
                     /translation="MPFFGSNTENIVKFEHEVLIVCVGRYLDYVTIRAFIKISLPGGI
                     LFLKTVGFGFVWGQSRPNWSNR"
     gene            2345..3598
                     /locus_tag="WIB82_001617"
     CDS             2345..3598
                     /locus_tag="WIB82_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ3842894.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3753..5264
                     /gene="lysS"
                     /locus_tag="WIB82_001618"
     CDS             3753..5264
                     /gene="lysS"
                     /locus_tag="WIB82_001618"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ3842895.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDTEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5341..5529)
                     /locus_tag="WIB82_001619"
     CDS             complement(5341..5529)
                     /locus_tag="WIB82_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ3842896.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPKISRMLSSILAGYAWDTENPFVAK
                     SEQRLSALSEWVGQLETE"
     gene            complement(5533..6411)
                     /locus_tag="WIB82_001620"
     CDS             complement(5533..6411)
                     /locus_tag="WIB82_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3842897.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNRSVADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSVIFGVVLEVKKRF
                     MSAAQARRRVDSDYRP"
     gene            complement(6441..>6977)
                     /locus_tag="WIB82_001621"
     CDS             complement(6441..>6977)
                     /locus_tag="WIB82_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3842898.1"
                     /translation="PFTDGNGRTGRILNSLLLIDKGLLDLPILYLSRYIIENRADYYR
                     LLLGVTERQDWESWIIYILDGVADTADWTVAKIDAIRRLFEQTRQHIRTHAQGIYTHE
                     LVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELSDIGVLQEIAIGRDKLFIHPRLME
                     LLRGEGNSFTSFQSLVKA"
BASE COUNT         1521 a         1804 c         1966 g         1686 t
ORIGIN      
        1 tgcttgcggc aagatgaaaa cggtgcggga tgttttagga aaccaatgcc gtctgaaggc
       61 tttcagacgg cattttttgc gccgtgctgt ttaacgcgcc agcggttcgg tgcgtttgat
      121 cagccacgct tttgccgcgc cttcggtcag cggctcgagg cggcggcgga cttcggcgtg
      181 gtagcggttg acccagtcga tttcgccgtc ggtcatgagg gcggtgtcca tcaggcgtgt
      241 atcgatgggg cagagggtca gggtttcaaa acagaggaag ctgccgaatt cggtttcttg
      301 aggggcggcg acggcttggt tggcggcaag gttttcaatg cggatgcccc attttcccgg
      361 gcggtagagt ccgggttcga tggaggtaac catgcctttt ttcatggcgg tttcgggcgt
      421 ggcgggggcg gcgaaggcga tgcgctgcgg gccttcgtgg acgttgagga aatagcctac
      481 gccgtgtccg gtgccgtggc cgtagtcgca ttgcgcctgc cacaggggtt tgcggcaaat
      541 cgcatcaatc agcggcgagg ggatgttttc ggggaacacg gcttcggcaa gcgcgatatg
      601 ggctttgaga acgagggtgt tgtcgctttt ttgttcggca ctcggcgtgc cgacggggac
      661 gacgcgggtg atgtcggtcg tgccgccttt gtattgcgcg ccggagtcga tgagcaaaag
      721 cccgttgccg ctgatggtgc tgtggctttc gggtgtcgcg ctgtaatgcg gcagtgcgcc
      781 gttggcgttg aagcctgcga tggtgtcgaa actcaatgaa atgaagcctg ggcgcacgct
      841 gcggtggcga taaagcatgg tgtccacgtc gatttcggtc aggctgccgc cgttgccgat
      901 gatgtcttca aactcggcga agaaaccgca caacgccgcg ccgtcgtgtt ccatcgcttc
      961 gcggatgcgg gcgatgtcgg cttcggattt gcaggatttg aacagcgtgg acgggttgat
     1021 tccctcgata aggcacacgc tttcgggcag gcgcacaagc gtgctgacgg cggttttgtt
     1081 cggttcgata agcaacgcgc cgccgatttg cgcgagtttg tcggcaactt gggcgtaagg
     1141 ctcgaccgtg atgcctgcgg tttgcagcgc ggcggcggct tcggcattca gacggcatcg
     1201 gtcggtaaac aggacggcgt tgtctttgcc aatcaataga taggacacga aaacgggatt
     1261 gaaaggcacg tcgctgccgc gcaggttggt cagccaagcg atgtcgtcaa gcgaggaaac
     1321 caagtggtaa tccgcgcctt tttccgccat gacggcgcgc acgcgggcga gtttttcggc
     1381 ggcggtttcg gagacgtagg cggggtcgtg gatgaacacg gtttcggcgg ggatagcggg
     1441 acggcttgtc cacacttggt caagcagatc gtccgggtgt tcgatgcgga tgtttttggc
     1501 ggtcagggat tgcgccaaag tgcgtttgcc ggtgagcgag accatatcgg aagggatgcc
     1561 gacggcggcg ttttcgggca ggtttgccgc gagccattcg ttatacggcg gcacttgccc
     1621 gcttttttgc agttcgatgc cgctgcccgc aagctgcttg gcggcttgtt cccaatagcg
     1681 gctgtccacc catacgcccg cttcatcggt ggtcaggaca aacgtgccga ccgagccggt
     1741 aaagcccgat aattcgcggc gcgcctgcca atgctcggac aggtattcgg acaggtgggg
     1801 gtcggcggaa gggatgacga gtgcatccaa gccttgcgcc ttcatggctt cgcgtaatgc
     1861 ggacagataa ttcgatacgg tattcataac agttcctcca atcgggtttt gcggcttcag
     1921 acggcatgga cggaaatctg aaatgccgtc tgaaaaacat aaccatctta tcaaatcgcc
     1981 agcctgctgc aaaacggacg ggctgatcgg atggtgcgtg tttgcgcaaa aaaagcgatg
     2041 taaggttgtt tgttgtaaaa ttttaggcat aaaatgtcgt tttattcctt tttttggaag
     2101 taatacggaa aatatagtca agtttgagca tgaagttcta atagtgtgtg ttggcaggta
     2161 tttggattac gttacaatac gcgctttcat caaaatttcc ctaccgggcg gtatcctgtt
     2221 tttgaagacg gtaggatttg ggtttgtttg ggggcaatcc cgcccaaatt ggagcaatag
     2281 atagggctgt gtccgaatat gcggctctgt gtgttgaaac acagttaaaa atacggaaga
     2341 ctttatgtcc gaacaacata tttcgacttg gaaaagtaaa atcaacgcat tgggtccggg
     2401 gatcatgatg gcttcggcgg cggtcggcgg ttcgcacctg attgcctcga cgcaggcggg
     2461 tgcgctttac ggctggcaga tcgcgctcat catcatcctg accaacctct tcaaataccc
     2521 gtttttccgc ttcagcgcgc attacacgct ggacacgggc aagagcctga ttgaaggtta
     2581 tgccgagaaa agccgcgttt atttgtgggt attcctgatt ttgtgcatcc tctccgccac
     2641 gattaacgcg ggcgcggtcg ccattgtcac cgccgccatc gtcaaaatgg cgattccctc
     2701 gctgatgttt gatgccggca cggttgccgc cttgattatg gcatcctgcc tgattatttt
     2761 ggtgagcgga cgttaccgcg ctttggatcg cgtttccaaa atcatcatcg ttactttgag
     2821 tatcgccacg cttgccgccg ccggcatcgc tatgtcgcgc ggtatgcaga tgcagcccga
     2881 ttttatcgag ccgacaccgt ggacgcttgc cggtttgggc ttcctgatcg cgctgatggg
     2941 ctggatgccc gcgccgattg aaatttccgc catcaattct ttgtgggtaa ccgaaaaaca
     3001 acgcatcaat ccttccgaat accgcgacgg gatttttgat tttaacgtcg gttatatcgc
     3061 cagtgcggtt ttggctttgg ttttccttgc actgggtgcg tttgtgcaat acggcaacgg
     3121 cgaagcagtg cagatggcgg gcggcaaata tatcgggcaa ttgatcaata tgtacgccgt
     3181 taccatcggc ggctggtcgc gcccgctggt ggcgtttatc gcgtttgcct gtatgtacgg
     3241 cacgacgatt accgttgtgg acggctatgc ccgtgccatt gccgaacccg tgcgcctgct
     3301 gcgcggaaaa gacaaaacgg gcaacgccga attctttgcc tggaatattt gggtggcggg
     3361 cagcggtttg gcggtgattt tttggtttga cggcgtaatg gcgaatctgc tcaaatttgc
     3421 gatgattgct gcttttgtgt ccgcccctgt gtttgcctgg ctgaattacc gtttggtcaa
     3481 aggtgatgaa aaacacaaac tcacatcagg tatgaatgcc cttgcattgg caggcttgat
     3541 ttatctgacc ggttttaccg ttttgttctt attgaatttg gcgggaatgt tcaaatgata
     3601 acgatgccgt ctgaaaccgc aaaccgcttt cagacggcat tgccgcgttt atggaaaaaa
     3661 atgccgacat cgcgtaaaat atgcgcaaat tttgtgattc ggtcaggtcg tctgaaacag
     3721 attgcgcctg atttattttt tcggaaaacc ctatgagcga acaaaaccat ccgcaaaccg
     3781 agccgcagtt ggacgaaaac caaatcatcg ccctgcgccg cgaaaaactg cacaacatcc
     3841 gccaacagcg caacgcctat cccaacgact tcaaacgcga cagcttcgcc gccgatttgc
     3901 acgcccaata cggcgaaatc ggcaaagaag aactcgatcc gcaaggcatt cccgtcaaag
     3961 tggccggccg catgatgctg aagcgtcaaa tgggcaaggc gagctttgcc actattcaag
     4021 acgtgtccgg acaaatccag ctttatctga acaacaaagg cgtgagccaa gaagttttgg
     4081 acgacttcaa ccattgggat ttgggcgaca tcgtcggcgc ggaaggcact ttgttcaaaa
     4141 ccaaccacgg cgaactgacc gtacgcgtgt ccggcatccg cctgctgtcc aaatccctgc
     4201 gtccgctgcc cgacaaacac aaaggtttga gcgatcagga aaccaaatac cgccaacgct
     4261 atgtcgattt gattgccaac gaagaatcgc gcaatacctt tatcaaacgc agccaaatca
     4321 tccaatccgt gcgtaatttt atggtgggcg agcattatct cgaagtcgaa accccgatga
     4381 tgcacccgat tcccggcggc gcgacggcaa aacccttcgt tactcatcac aatgccttag
     4441 atattccgct ttacctgcgt atcgcgcctg agctgtattt gaaacgcctg gttgtcggcg
     4501 gcttggaacg cgtgtttgaa atcaaccgca gcttccgcaa cgaaggcatg tccgtgcgcc
     4561 acaaccccga atttacgatg attgaatttt acgaagcctt ctccgactac gaacgtatga
     4621 tgcagatggc ggaagacatc atccgcaacg catcgcgcac agtaaacggt acggcaaaca
     4681 tcacttacaa tggcaaagaa gtcgatttgg aaagcccgtt cgagcgtctg accatcctcg
     4741 aagccatcaa aaaatacaat ccgcactaca ccgacgagca gttgaacgat acggaatggc
     4801 tgaaaaaaga aatcgtcaaa cacggtgaaa gcctgccgcc gtccccgggc atcggcagcc
     4861 tgcaactcgc gctgtttgaa ggttgcgccg agggcaagct gtggaacccg acctttattg
     4921 tcgattaccc ggtcgaagtt tccccgttgg cacgcgcttc ggatacgaaa caaggtctga
     4981 ccgaacgctt cgagcttttc gttgtcggcc gcgaactggc aaacggctat tccgagttga
     5041 acgaccccga agaccaagcc gaacgcttca aagcgcaagt ggcgcaaaaa gacgcgggcg
     5101 acgacgaagc gatgcactac gatgccgact acatccgcgc gatggaattc ggtttgccgc
     5161 cgaccggcgg ttgcggcatc ggcatcgacc gcttagtgat gctgctgacc gattcgcaaa
     5221 ccatccgcga tgtgattctg tttccgcaga tgcgccccga gtaatcataa aaacagttga
     5281 aatagaaatg ccgtctgaac ccgattggat tcagacggca ttttgtatgg cggtacggat
     5341 ttattcggtt tccaactgac cgacccattc ggacaaggcc gacaggcgtt gttcggattt
     5401 cgccacgaac gggttttcgg tatcccacgc gtagcctgcc aaaatcgaag aaagcatacg
     5461 gctgattttc gggctgcggt cgggcgcgta cacgacgttt tggaagcgga aatcgtacca
     5521 accgtccaca tatcaggggc ggtaatccga atcaacacgc cttctcgcct gtgcggcgga
     5581 cataaaccgt tttttgacct ccagcaccac accgaaaatc acgctgcctt cgggcaggtc
     5641 aaaggcttca gacggcctgc ccgtctgata aatcagcatc ctgcccgcaa tatcgataac
     5701 aaggtgcgcg acgaccagcc gcttgtcgga cagttcaatc agcacgatgt cttcgtccgc
     5761 agggcgcata cgcggctcga ccaccagcaa atcccctttt tcgataaccg gcgacatgga
     5821 cgtgtcgggc atggcaacgg caaacgtata ttgtgaaggg gcgaccaccg atgcaaacaa
     5881 gtcgaaactg cccgtgagtt tgcctgtacg ggcgaaagcc gccgcctgac ggtctgacaa
     5941 caaaggacgg ggtgtgagcc tcatcgacag tgctttgccg tcggcaacgc tgcggttgtg
     6001 cgtatgcgta tgatggttgt tgatggtcgt atcgccttgg acatcgttaa ggactatgct
     6061 gtttgaacct gccacggatt tggtcatctc attgtaggct tgcgtcgtga tgcgctcttg
     6121 cttggtcata cctgttacag cctgtttcag catttctaac gctatgtgat tcttcctaag
     6181 ggtcgctata ccggcttttg cagcttgctc attaccacaa gaggggcaac cccagctact
     6241 tcctatgaac atattggagt acctcgaaca ggtttggttt ccatgtttag ggcagttgat
     6301 tacacacggc ttggctgtac cctcaaactc gaccaactta atgccacgat gaccgaaacg
     6361 ttcctcaacc tcagcagcag cttcttcaaa tgtttttgac cttcttgcca tattgacctc
     6421 cggattattg attattttgg ctatgcttta acgagggatt ggaatgaggt aaagctgttg
     6481 ccctctcccc gcaatagttc cattaggcgc ggatgaatga atagtttgtc cctgccgatg
     6541 gcgatttctt gcagcacacc tatgtctgag agctctttca gatacttaga agccgtctgc
     6601 cgtttggcta tccctgccgc ttctaggttg gcaatgcgtg tatatggctg ttcaaacaga
     6661 agatttacca gttcgtgcgt gtagattcct tgtgcgtgtg tccgtatgtg ttgccgtgtc
     6721 tgctcaaaca ggcggcgtat cgcatctatt ttcgctaccg tccaatcggc ggtatcggct
     6781 acgccgtcta agatgtagat tatccagctt tcccagtcct gccgttcggt tacgcctaaa
     6841 agcaggcggt aatagtccgc cctgttttcg ataatatagc ggctcaaata caaaataggc
     6901 aaatccaaaa gccctttatc aatcaatagc aggctgttca atatgcgccc cgtccgcccg
     6961 ttgccgtccg taaacgg
//
LOCUS       DAWHRS010000114         6924 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWHRS010000114 DAWHRS010000000
VERSION     DAWHRS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.114"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..353)
                     /locus_tag="WIB82_001622"
     CDS             complement(<1..353)
                     /locus_tag="WIB82_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ3842899.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICAPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDY"
     regulatory      568..612
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            617..1090
                     /gene="queF"
                     /locus_tag="WIB82_001623"
     CDS             617..1090
                     /gene="queF"
                     /locus_tag="WIB82_001623"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ3842900.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            1167..1853
                     /locus_tag="WIB82_001624"
     CDS             1167..1853
                     /locus_tag="WIB82_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ3842901.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(2356..3648)
                     /locus_tag="WIB82_001625"
     CDS             complement(2356..3648)
                     /locus_tag="WIB82_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ3842902.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAEGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            3725..4693
                     /locus_tag="WIB82_001626"
     CDS             3725..4693
                     /locus_tag="WIB82_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842903.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWGGTNGGTQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(4894..6360)
                     /locus_tag="WIB82_001627"
     CDS             complement(4894..6360)
                     /locus_tag="WIB82_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3842904.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(6602..>6924)
                     /locus_tag="WIB82_001628"
     CDS             complement(6602..>6924)
                     /locus_tag="WIB82_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ3842905.1"
                     /translation="TDSEGTAVGLLGSGQWQSRAGIRAKTRFALRNGVNLQPFAAFNV
                     LHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGWKGHMSARIGYGKRTDGDKEAALSL
                     QWLF"
BASE COUNT         1548 a         2093 c         1676 g         1607 t
ORIGIN      
        1 tgataatctt caaattccca cgaaaaaaaa cgaagtaaat aagtcaatga cttttcccaa
       61 gtttcttttg aacattcttt aagaattttc tcaatttccg atttaataac agaatgatta
      121 aattcattca taatcatcat acccgctccc catttaaccc tttgattttg gaaacaatta
      181 tgcaaaatcc atttaggagc gcatatgcga acagaaaata tatctgcagc atcactatca
      241 tcagttccta tgtctaaatc aattcccaca caaaaattgt ctttgatttc gggaacgaaa
      301 tcttcaaagg cacaatcgta aagattgatg gctttcaatt ctaggttaat cattttatat
      361 tcaaaataaa taaaaccaat taaagattgg ttaggattct ataccctggc aataactcaa
      421 tcaagttaat gatttagaat gaatattcaa ataaactgta tcagcccggc ctcacagaac
      481 tatttatatc cacacatcac cgagtaaagc aattacccat actaaccgaa gaaaactaca
      541 caaataccgc cacttatatt acaatcgccg cccgtggttc gaaaacctcc cacattaaaa
      601 aactaaggaa accctcatgt cccgcaacaa cgaagagctg caaggcatct ctcttttggg
      661 caatcaaaaa acccaatatc cgaccggcta cgcgcctgaa attctcgaag cattcgacaa
      721 caaacatccc gacaacgact atttcgtcaa attcgtctgt cccgagttca ccagcctctg
      781 ccccatgacc gggcagcccg acttcgccac catctacatc cgctacattc cgcacatcaa
      841 aatggtggaa agcaaatccc tgaaactcta cctcttcagc ttccgcaacc acggcgattt
      901 tcatgaagac tgcgtcaaca tcatcatgaa agacctcatt gccctgatgg atccgaaata
      961 catcgaagta ttcggcgagt tcacaccgcg cggcggcatc gccattcatc ctttcgccaa
     1021 ttacggcaaa gcaggcaccg agtttgaagc attggcgcgt aaacgcctgt ttgagcacga
     1081 cgcacaataa accatccttc ccatatttca gacggcattc cttcgatacg atatgccgtc
     1141 tgaacgcctt tccgaaaggt ttttttatgt acgcctttac cgacgcacag caacagaagg
     1201 cactcttccg gctggtgctt tttcatatcc tcatcatcgc cgccagcaac tatctggtgc
     1261 agttcccctt ccaaattttc ggcatccaca ccacttgggg cgcattttcc tttcccttca
     1321 tcttccttgc caccgacctg accgtccgca ttttcggttc gcacttggca cggcggatta
     1381 tcttttgggt gatgttcccc gcccttttgc tttcctacgt cttttccgtt ttgttccaca
     1441 acggcagttg gacaggcttg ggcgcgctgt ccgaattcaa cacctttgtc ggacgcatcg
     1501 ccttagccag ctttgccgcc tatgcgctcg gacaaatcct tgatattttt gtattcaaca
     1561 aattacgccg tctgaaagcg tggtggattg ccccaaccgc atcaaccgtc atcggcaacg
     1621 ccttggatac gttggtattt ttcgccgttg ccttctatgc aagcagcgat gaatttatgg
     1681 cggcaaactg gcagggcatc gcttttgtcg attacctgtt caaactcacc gtctgcggtc
     1741 tgtttttcct gcccgcctac ggcgtgattc tgaatctgct gacgaaaaaa ctgacgaccc
     1801 tgcaaaccaa acaggcgcaa gaccgccccg cgccctcgct gcaaaatccg taacacagcc
     1861 ctgccgggaa tcgggacggt tgggttttct tttatttgca aacactgtcg gaacggataa
     1921 atccggattc ctacctactt cgtcattccc gcgaaagtgg gaatccggtg cgttgggttt
     1981 ccatcatttc cgataaactc ctgccgcgtt gtatttccag attcccgctt tcgcgggaat
     2041 gacggcggat gggtttccgt accgtggtgt ccggattccc gacggtacgg gaatgacggc
     2101 agtgcttcct tggcttagtt ttattttaat cgactgtatg gtggattaac aaaaatcagg
     2161 acaaggcaac gaagccgcag acagtacaaa tagtacggaa ccgattcact tggtgcttca
     2221 gcatcttaga gaatcgttct ctttgagcta aggcgaggca acgccgtact gatttttgtt
     2281 aatccactat aaatgccgtc tgaaaacctg tttgcaggtt ttcagacggc attgttttcg
     2341 tcgccggttc aaacttcaat ccgattgcga cacggtaacc ggtatgccgc gcaagcgcgc
     2401 aagcagcgcg tccgccttct gtttctgcgc ggcaaacgcc gccttgtccg cctgcgtcaa
     2461 ttccggtgtc ggcaatgcga ccgaaacagg attgacgggc tgcccgttga tgcgcgcctc
     2521 gtaatgcagg tgcggcccgg tcgaacgccc ggtcgaaccg acaaaaccga tgacctcgcc
     2581 gccgcgcaca ttgccttccg cctgcgaaaa cgcgctcaag tgcgcgtaca gcgtttccac
     2641 accgttggcg tggcgtatca tcaccgcgtt gccgtatccg cccttccggc ctttaaaggt
     2701 aatcacgccg tcggcggaag ccctgaccgg cgttccctgc ggtgcggcat aatcgatgcc
     2761 tgtgtgcagc cgccatgtgt gcaggatggg gtgcatacgg tagccgaacg gcgaagaaat
     2821 gcgcgtatag accagcggct cgatgttgaa gccgcctttt tcctgcaaca ccctgccgtc
     2881 ttcatcataa taattgccgc cccctccgcc ttccttgtcc gaacggtaat agaatgcctg
     2941 atgccttgtg ccgcccttaa cgacttcagc cgccaaaata tcgcccgccg ccacctgctg
     3001 cccgtggaaa tacaggctgt cgtacatcag gcgcacggca tcgccttcct tcaaaccgtc
     3061 aaggctgaag cggccggcga aaatcccgct taaggattcg cggatttcga cgggcacttc
     3121 cgcccgcgcc agcgaaccgc gcgccgacgt tttgaccacg accgaacgca gcgtcggcaa
     3181 aaccttcata tccgcctcag aagccgaccg ccgccatatg ccgcctttct tttccaaagc
     3241 gaccagattg cgctcgccgt cttcgtcggt aaaaaactgt acttcgcgcg cgccgccgtc
     3301 gccgccgacc aaaacatgaa ccgactggtc ggcacgcaaa tgccgcaaat cggcttcgcc
     3361 gccatatttt tccgtgattc gggcaatctc gtcccgcgcc atacccgaac gcgccagcac
     3421 gtccgccagc gagtcgcccg gctgcaccgc ctcctgcacc caatatgccg tctgaacgcc
     3481 gctgccgccc caagacagcg gcggcagatt ttgttccacg cgctgcggtc tgacgcgttc
     3541 cgtcctctct gtcaaagcaa tgtatgccgc cgacaccaaa ataatcgaaa cggcaagcgc
     3601 gcgcagcgcg tatttccgat gttttgccga aagtgggaag acagccatat tgttcctttt
     3661 tgacggcgtt tgcgccgaat cctctataat atccgcccga tccgcatttg tcagacatca
     3721 aacgatgaaa ctcaccctcg ccctcccctc gctcaatttg gatgaagacg aaatccgaat
     3781 acctctatgc ctgcccgcct tcaacaaaat cctgcaatac ggctcgctgc accgccaaag
     3841 ctgcacggct tcggcatttt acgcgcgtta tttgtggtgc ggcagactgg cggaacgcgc
     3901 ggcgcaaagc ctgaatatgc cgtctgaaac cgtcgcgctc gccacgcccg tctggcaaaa
     3961 gatggggatg catcaggcaa atgtgctgac ggcggaatat ttggatgtcg gtacggatga
     4021 agcggaacgg ctctgccgcg atctgtcggc gttttacggc gatattccgt ggcgtttcgt
     4081 tcccgtcctg cccgaattat ggctggtttc ccttccccgc gcgtaccgct ggggcgcgaa
     4141 gcccgttttg gacttgggcg gattgttggg cgcggacgat cagccggacg gagaagatgc
     4201 gttggaatgg ctgcgcgtcc aaaccgaaat ccaaatgtgg ctggcggcgc accccgtcaa
     4261 ccataaccgc aagaaacgcg ggctgcccga gttgaacggc ttatggcttt ggggcggcac
     4321 aaacggcggc acgcaaggcg gcacgctttt cgccgatacg gtttggagcc gtttccaccc
     4381 cgaccgccgc gcattgcccg acaacttccg cgcctatgaa gaaacggcgg cacacctgcc
     4441 cgacacgcac catatcctgt ttatggacga tttgcgcctg accgccctga caggggacag
     4501 ggagcggtat gcggcaatat tgcagcagtg ggaagaacgc tggttcgcac cgctttacga
     4561 agccgtccgc atcggcaaaa tcaaacggct ggacatcgcc acggacggac aacacggcgg
     4621 tacgctgacc ttcaaacccg ccgacaggtg gaaattttgg cgcggcgcga aaacctttga
     4681 cggaatctgg tgaacccgcc cgccgcaacc gcgacaatgc cgtctgaagc ccgaatcggg
     4741 tttcagacgg cattttacct cgcatcgccg ttatgccgga aaaaccggaa tgcgtttccg
     4801 cacacgcggc ggcacgcaaa gcaccgcccg aaagccgccg atgcccgccg tgataccgcc
     4861 gccggcaggg tttcctccga aaaggaacag caatcagaac gttttattaa actcgacaaa
     4921 cgcccgattt ttctcgtatt cgttgaacac atcgttactc cgcgtttcgc ggtgcgacaa
     4981 cgtcaggcgc ggcgtgatgc ctttgaaatg caatgcccgg tgccaaaggc tcaaggatgt
     5041 gttcaattct ttatccctgc gcctttcccc tttaaaaccg ctgaaaaagc cgggtttttc
     5101 ataatgccgt ttcgccgcgc cgaggcgcaa cagcgaagac aggccgctgc cgccccattc
     5161 ctgcccccag gcaaagcgca ggccgtaacg gttgaaattg tcgccccggt cggcggggtt
     5221 gcgctcgcgg taaaaatcca aaccgcccat ccaatattgg cgcgcattcc ggtaaaacac
     5281 cagcgaattg gaaatttgca aatgggtatt gtcggaacgc gcccggcgcg tattcttcaa
     5341 acgcccccac tccgccgaag acaacgtttg ccatttcggg gtttgccaac ggttgaaata
     5401 aaggcgtgcg ccgttggtgt aagaataagc gtcgttgccg taggtgcggc gttcgtggaa
     5461 cactgccagc ccggcatctt tgcgccggtc ggcaaaaccg atgccgccgg aaacgcctgc
     5521 cgtcatatcg ttgaatttct tattccccgg ataaaccctg ccggacacgt cgccgcccgc
     5581 cgtcgtgtac cagccgtttt tcagcgacca ttttttctcc gcgccgagcc ggtaattgac
     5641 cgccgtgccg tccacctgtt tcgggaaagt ccatttgccg tactgctgcc gtttcggggc
     5701 ttggttgata ttgtgttcgc gggtgacgct gaagccgcca tttaccttcc acgcatcgcg
     5761 ttcgcgcaat gccttgcggt acagctcgac ctgctccatc agctgcggcg gcaggttttc
     5821 cgccttcagg cggtcgaact ggtctgccgc cgcctcgttc tgcctgtttt caaacaatgc
     5881 cgccgccaaa cgcatacgga cggcgggcgc gtcgggttgg gcggcaatca attcccggta
     5941 atgggaaatc gcctccttca ccctaccgtc tgcctgcgcc aaaatccctt gtgcataaag
     6001 tgccaacatc ttatcctgct gcgcctgttg taggtaaatc ggcaaaataa cgcggatacc
     6061 ggcaatattg tttgagacca ctgcggaata catcgcgcgg gacaacaatt cgggattttt
     6121 cagcagggtt tctccgtcaa tctgcaaaac ttttcctttt tcccgcacct gccccggtac
     6181 cttctccctg tcgattggtt tgacctccgc ttcatgaagc ctgaactcgg gacggcttct
     6241 caaatccggt tcgcgcggtg tttcttcggc atatgccgcg cttgccaata aaggcagcag
     6301 cagcatccat ctgtttcgtg caggcataaa tgtcttccca caaaaataaa aaataaccat
     6361 tatcaatcgg cggattgtat cagtttttta tgccgctgtt ccaaaccctg ccgcccgatg
     6421 ccgcgacaat gccgtctgaa gccagaatcg ggtttcaaac ggcggcaaaa aaccggcggt
     6481 tttccgccgg tttcatcatg atgccccgcc cttcggggcg gcagggttcg gattggtttg
     6541 cggggcggcg cgcggggccg tgccggtgtg ccgcctgtgc gtcaaaacat ttcccgacgc
     6601 atcaaaacag ccattggagc gacaatgcgg cttctttgtc gccgtccgtc cttttgccgt
     6661 atccgatgcg tgcggacata tggcctttcc aaccggcttc aatgccgaac cgcccttcga
     6721 gcgccgtcct gcctgccagc gtctgttttt cgccgtccat ttccacgccg aaagattttg
     6781 acctgtgcaa aacattaaaa gcggcaaaag gctgaagatt gacaccgtta cgcaaagcaa
     6841 aacgggtttt tgcccgaatg ccagcgcggc tttgccactg accgctgccg agcagtccga
     6901 ccgccgtccc ctcgctgtcg gtaa
//
LOCUS       DAWHRS010000115         6888 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWHRS010000115 DAWHRS010000000
VERSION     DAWHRS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.115"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            147..1007
                     /gene="cysW"
                     /locus_tag="WIB82_001629"
     CDS             147..1007
                     /gene="cysW"
                     /locus_tag="WIB82_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ3842906.1"
                     /translation="MKPYSANPNLTEPRWLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            1004..2077
                     /locus_tag="WIB82_001630"
     CDS             1004..2077
                     /locus_tag="WIB82_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ3842907.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(2134..3660)
                     /gene="ilvA"
                     /locus_tag="WIB82_001631"
     CDS             complement(2134..3660)
                     /gene="ilvA"
                     /locus_tag="WIB82_001631"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ3842908.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            3808..4977
                     /locus_tag="WIB82_001632"
     CDS             3808..4977
                     /locus_tag="WIB82_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ3842909.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVEKRSRWQRLWACLTGQ"
     gene            complement(5102..5674)
                     /locus_tag="WIB82_001633"
     CDS             complement(5102..5674)
                     /locus_tag="WIB82_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ3842910.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(5741..6724)
                     /locus_tag="WIB82_001634"
     CDS             complement(5741..6724)
                     /locus_tag="WIB82_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ3842911.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
BASE COUNT         1798 a         1927 c         1644 g         1519 t
ORIGIN      
        1 ccgcctgcgc gggaatgacg gtagctagac gtttttattc ccttaatcaa taaaaggttg
       61 tctgaaaacg aatccgcccc acaaaaaacg gtttttcaga cggcatccga acaccttaaa
      121 acaaccaacc ggggaacacc accgccatga aaccctattc cgccaatccc aacctgaccg
      181 aaccgcgctg gctgcgcgtg ttgctgattg ccgccgcgct gggctttctg ctgctgatgc
      241 tggtcgtgcc gctcgtcgcc gtgttttacg aagccttaaa aggcggttgg gatttgtacc
      301 tgaaatcctt aaacgatccc gaagcgtggt ctgccatcaa attgacgctg attaccgcgc
      361 tgattgtcgt ccccgtcaat gccgtcttgg gcgtggcgat ggcgtggctg ctgacccgtt
      421 ttgattttcg cggcaagcag ttgctgacta ccctgctcga tttgccgttt tccgtatcgc
      481 ccgtggtggc cggtttaatg ttcgtcttat tgttcggcgc gcacacggca ttgggtggct
      541 ggctcgaagc gcaaggcata cagattatct tcgccatccc cggcattatt ctggcgacgc
      601 tgttcgttac tttccccttt gtcgcacgcg aaatcatccc gctgatgcag gctcagggcg
      661 acagcgaaga acaggcggca ttgatactcg gcgcaagcgg ctggcagatg ttttggcgcg
      721 ttaccctgcc caacatcaaa tgggcgttac tctacggcat catcctcacc aacgcccgcg
      781 cgatgggcga gttcggcgcg gtcagcgtgg tatcgggaca catacgcggc gaaaccaaca
      841 ccgtcccgct tttggtcgaa atcttctaca acgaatacaa cttcaccggc gcattcgccc
      901 tctccggcgt attggcactt ttggcactgg cgacgctggc ggtgcagaac atcattacca
      961 aattacaaga caaaaaactc gccgccgccg aaaggaacgc agtatgagta tcaccatcca
     1021 aaacttaaac aaacacttcg gcaattttca cgcgctgaaa aacatcaacc tcaacgtccc
     1081 caccggcaaa ctcgtttccc tgctcggccc gtccggctgc ggcaaaacca cgcttttacg
     1141 cattatcgcc ggactggaaa acgccgacgg cggcaagatt ctgtttgatg ggcaagacgt
     1201 aaccgccaag cacgtgcgcg agcgcaaagt cggcttcgtg ttccaacact acgccctttt
     1261 ccgccatatg aacgtgtttg acaacgttgc cttcggtttg accgtattgc ccaagtccga
     1321 acgcccgtcc aaaggacaaa tccgcgccaa agtcgaagaa ttgctgaaac tcgtacaact
     1381 ctcgcatttg gcaaaatcct atccgcacca actctccggc gggcaacgcc agcgcatcgc
     1441 cctcgcccgt gcgcttgcgg tcgaaccgaa actcctgctt ttggacgaac ccttcggcgc
     1501 gttggatgcc aaagtacgca aagaattacg cacctggctg cgcgacatcc atcacaacct
     1561 gggtgtaacc agcattctgg ttacgcacga ccaagaagaa gccctcgaag tttccgacga
     1621 aatcgtcgtg atgaaccacg gcaaaatcga acaaaccggc agcgccgaag ctatttaccg
     1681 caaacccgaa aatgccttcg ttaccgagtt cctcggcgaa accgacgctt ttgaaggacg
     1741 catcgaaaaa ggcttctggc attataacgg cttcgcgtgg aaattggacg cacaatacaa
     1801 atggcaggaa caaaccgcca ccggctatat ccgcccgcac gaatggcaga tcgccgccga
     1861 acacgaaaca ccgatgattt gtgccgaaat cgaaaaagtc cacgccgtcg gggcattgac
     1921 gcatattttg gtgaaacacg gcaaacaaga cgtgcatatc acgctggctg gcagcgatgc
     1981 cgcgcgttac ccaatcgccg aaggcaaaga attgaagctg attccgaaac aggtttatgt
     2041 cttctctcaa aacgaactga ttgaatattc gatttaacca tgaaagcgca atgccgtctg
     2101 aaaggctttc agacggcatt gtgctttcaa gcgtcaggca agaaacagct tgtacgcggc
     2161 attttgcgtt tcctcgtgat agctgtatcc cagactttcc aagaaaccgt caaatgcggc
     2221 ggcatcgtgc ggcggcacat cgataccgac caaaatccgc ccgtaatccg caccgtggtt
     2281 gcggtaatgg aagagcgtaa tgttccaacc gccctgcata tggttcaaaa aacgcgccaa
     2341 cgcccccgga cgctccggaa actcaaaact gaccagacgc tcgttttcta ctttgtccgt
     2401 ccgccctccg accatatagc ggatatggat tttggcaatc tcatcatcgg tcaggtcgac
     2461 attgggcaat cccgcctcat ccaaccggct gccgataacc gccaaatcct gcgggcctgc
     2521 cgcttgaagt ccgacaaaga tatgcgcttt ttcatcgtcc ccgtagcggt agttgaactc
     2581 ggtaatattc ctatttccca atatattgac aaacttaagg aagctgccgc gttcttcagg
     2641 gatggtaacg gcaaaaatac cttcgttgcc ctcgcccaat tcgctccgtt ccgaaacgtg
     2701 gcgcaaacgg tgaaaattca tattcgcacc gctggtaacg gcaatcaggg tttggttttc
     2761 cgcgccttct cgggcgatat aggctttcag acccgccaac gccaacgcgc ccgccggctc
     2821 ggtaatgctg cgcgtgtcat cgaaaatatc cttgaccgcg ccgcaaaccg catcggtatc
     2881 gactgtaatg atttcatcca aaagttcttt gcagagacgg aaggtttcgt ttccgacgac
     2941 tttgaccgcc gtgccgtctg aaaacagccc gacatctttc aaatggacga tttcacccgc
     3001 ttcgaccgac tgcttcatac agcaggaatc gttggtctga acgccgataa ctttgatttc
     3061 gggacggacc tgcttgataa atgccgccac gcccgccgcc aaaccgccac cgcctatcgg
     3121 tacgaatacg gcgcggattg gatcgggatg ctggctgaca atttccatcc ctaccgtccc
     3181 ttgtcctgca atcacatcag gatcgtcaaa cggtgcgata taggtcagcc cttcctgttc
     3241 cgccagctcc atcgcataat cgtaagcatc gttgtatgaa acaccgcgca aaaccacctc
     3301 gccgccacgg ctcttaaccg catccacttt gattttcggc gtagtctccg gcataacgat
     3361 aacggcacgg cagcccaaac gctgtgcgga caatgccacg ccttgagcat gattgcccgc
     3421 gcttgccgca atcacgccgc aagcgagcgc atctttcggc aacttggaca ttttgttgta
     3481 cgcgccgcgt attttgaacg aaaaaaccgg ctgcaaatct tcgcgtttca aaaggatgtt
     3541 gtttttcaaa cgtacagaaa ggctgcgtgc cggttccaaa ggcgtttcga ccgcgacatc
     3601 atagacagat gccgtcagga tgcggatgag gtaattggaa taaggaaggg gcgtgttcat
     3661 aattcaatat gggataatcg gtttattaaa atcgcaaaac ccaaaaccat acgcccaaga
     3721 cggcgcgaaa gcaagaaaaa tccgcccgat cagacaccct aagcgtataa tcggcagact
     3781 gaaacacgca cacaattaga atatttcatg acagcacata aaatcctgcc cgtcctgctt
     3841 tccatcatct taggcgtttc tcacgcaacg gccgcatcgc ccgcgcccaa caaaccggcg
     3901 gtacacgccg cccccacgtt ccaaacaccc gaaaccctca cagcggcaca catcgttatc
     3961 gaccttcaaa gcaaacagat tttatccgcc aaaaacatca atacccctgt cgaaccggcg
     4021 gcactaaccc aactgatgac cgcatatctg gttttcaaaa acatgaaatc gggaaatatc
     4081 cgatctgaag aaaacttaaa aatacccgaa tccgcatggg cttcagaagg aagcagaatg
     4141 tttgtacgtc ccggcgatac ggtcagcacc gacaaactct taaaaggcat gattgcacta
     4201 tccgcaaacg atgccgccct aacccttgcc ggccggctgg gcaacggctc gattgaaaat
     4261 tttgtgcaac aaatgaacaa agaagcccga cgcttgggca tgaagaacac tgtattcaaa
     4321 aatccgacag gcttgagtag agaaggacag gtttccaccg ccaaagacct cgcccagctg
     4381 tctgaagcat tgatgcgcga ctttccggaa tattacccgc tgttttccat caaatctttc
     4441 aaattcaaaa atatagagca aaacaaccgc aatatccttt tatataggga caacaatgta
     4501 aacggtctga aagccggaca cacagaaagc ggcggctaca accttgccgt gtcatactcc
     4561 ggcaacggca ggcacatcct tgtcatcaca ttaggttcgg aatcggcgga aacacgcgcc
     4621 tcagacaaca gcaagctgct gaactgggca ttgcaagcct tcgatacgcc caaaatatat
     4681 ccgaaaggca aaaccgttgc ccaaatccaa atttccggag gcagcaaaaa aaccgtccgc
     4741 gcaggcttcc tcaaagaagc ctacatcact ctgccacata aggaagcgaa aatggcagaa
     4801 cagattctag aaaccataca gccgattccc gccccggtaa aaaaagggca gattttagga
     4861 aaaatcaaaa tcaggcaaaa cggatacacc attgccgaaa aagaaatcgt cgcactggaa
     4921 aatgtagaaa aaagaagccg gtggcaaagg ctttgggcgt gtctgacagg gcagtaatct
     4981 gccgtttcaa atatcccgtt tttccaacaa ataaagaaat gccgtctgaa acacggttca
     5041 gacggcataa aacaacaggg cggtacgtat tgcatacgca ccgccctgct gctgaaatca
     5101 attagcgttt cttaccggta acggtagcaa cagccagatt ttcgttacgt ttcagggaaa
     5161 cgctttctac accttcaggc agtttgatgt ctgacaagtg cagaatgtcg ccggcaacca
     5221 cttcggcaca atccaaatcc aagaaagcag ggatgttggc aggcaaagca actacttcaa
     5281 cagaagtgtt taacagagat acgcggccgc cttgcagttt gaccgcttgg gaattttcag
     5341 cgttaacgat gtgcagggga acgcggatgc gtacgggttg gtctgctttc acagcttgga
     5401 agtcgatgtg ttgaacttcg cggcggaacg ggtgcatttg gaaatcgcgg acgataacgt
     5461 ctttggtttc accgttcaga gacaacttaa tcaacgcagt atggaaagat tctttttcca
     5521 atgcgtagaa tacggttttg tgatccacag cgattgcaac aggctcttga ccttcaccgt
     5581 acagaatgcc ggggatttgg ccttcgcgac gcaggcggcg gctcgcacca gtgccttgtg
     5641 cttcacgaac agaggcttga atttcataag tcatgttaaa tactccaagt taggtaaaat
     5701 cgccgtcatc ggccgcgacc agcttaagac ggcttcgggc ttatggcagc aacatgctgc
     5761 ctgtcatcac ttcttcattg aaaagatatg agacggattc ttcattgcta atgcggcgga
     5821 cggtttcggc caacagaccg gcaatcgtta cctgacggat acggtcgcag tttttagccg
     5881 cttcagacaa aggaatggta tcggttacga ccacctggtc gatttcggat gaggcgatac
     5941 ggctgaccgc ctctccggag aatacggcgt ggctggcata ggctagaaca cgttcagccc
     6001 cccgctcttt cagggcgacg gcggctttgc acagcgtatt tgcagtgtca atcatatcgt
     6061 ccacaatcag acaggttcta ccttgaatat cgccgatgat gttcatgact tccgccacat
     6121 tggctttcgg gcggcgtttg tcgatgattg ccaagtcggc attcagggat tttgccacgg
     6181 cgcgggcgcg gacgacaccg ccgatgtccg ggctgacgac ggtcagattt tcaatccgct
     6241 gttgtttgat gtcgttcaac agaatcgggg tggcataaat attgtccacc ggaatatcga
     6301 agaaaccttg aatctggtcg gcatgcaaat cgacagtcaa aacacggtcg atccctgccg
     6361 aatacagcat atttgccacc agtttggcag aaatcggaac gcggacggaa cgcggacggc
     6421 ggtcttggcg cgcatagccg aaatacggaa tggctgtggt aatacgacct gccgaagcac
     6481 gcttcagtgc atccgccatc gtcaggattt ccatcaggtt gtcattggtc ggcgcacagg
     6541 tcggctgaag gatgaaaaca tcgcgcccgc gtacgttttc caacagttcg acggcaactt
     6601 cgccgtctga aaacttggat acggaagcat tgcccaaaga aatgtccaaa tgcctgacaa
     6661 cacgttgtgc caattcggga ttggcattgc ctgtaaatac catcaaactg tcgtacgcag
     6721 ccatattctc acctgatttt atgtttaact tccgctcaga aaacacaatg cttccctctg
     6781 cttgtaccaa gcccgacatt atacctgcaa catccgacac aaacaaaggc atttcaatat
     6841 ttttatttct atgaaataaa agcgtgtaag caggcttaca cgctttta
//
LOCUS       DAWHRS010000116         6782 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWHRS010000116 DAWHRS010000000
VERSION     DAWHRS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.116"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..368
                     /locus_tag="WIB82_001635"
     CDS             <1..368
                     /locus_tag="WIB82_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ3842912.1"
                     /translation="LLGMTSFWVGLAGILVYTVVQIYYMSRDGQSLGKKIMRIRVLKT
                     DGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLLAFLANFVLLFMVKR
                     DRRTLYDILADTVVVKLPK"
     gene            complement(430..1284)
                     /gene="folD"
                     /locus_tag="WIB82_001636"
     CDS             complement(430..1284)
                     /gene="folD"
                     /locus_tag="WIB82_001636"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ3842913.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1507..1584
                     /locus_tag="WIB82_001637"
     tRNA            1507..1584
                     /locus_tag="WIB82_001637"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1542..1544,aa:Pro,seq:tgg)
     gene            1609..1685
                     /locus_tag="WIB82_001638"
     tRNA            1609..1685
                     /locus_tag="WIB82_001638"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1643..1645,aa:Arg,seq:tct)
     gene            1713..1788
                     /locus_tag="WIB82_001639"
     tRNA            1713..1788
                     /locus_tag="WIB82_001639"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1746..1748,aa:His,seq:gtg)
     gene            2000..2506
                     /locus_tag="WIB82_001640"
     CDS             2000..2506
                     /locus_tag="WIB82_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ3842914.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2503..4281
                     /locus_tag="WIB82_001641"
     CDS             2503..4281
                     /locus_tag="WIB82_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ3842915.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4394..5218
                     /locus_tag="WIB82_001642"
     CDS             4394..5218
                     /locus_tag="WIB82_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ3842916.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(5981..6769)
                     /locus_tag="WIB82_001643"
     CDS             complement(5981..6769)
                     /locus_tag="WIB82_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ3842917.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPYTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         1431 a         1782 c         2039 g         1530 t
ORIGIN      
        1 tgcttttggg catgacttcc ttttgggtag gactggcggg tattttggtt tataccgtcg
       61 ttcaaatcta ctatatgagt cgggacgggc agtcattggg taagaaaatc atgagaatca
      121 gggtgttgaa aaccgacggc cgcaatcccg gttttgtcgg cacggttttg gtacgcgaaa
      181 tcgcatggtc ggttttggtt gccattattg ccgccgttat cggtcttgca gtaggtgaca
      241 acggagaaaa cgccatcaac ctgctggcat tccttgccaa ctttgtcctg ctctttatgg
      301 tcaaacgcga ccgccgcacg ctttacgaca tactggcgga tacggttgtc gtcaagctgc
      361 ccaaataaac ggacacggca aaatgccgtc tgaaagcctt tcagacggca tttttatctt
      421 cagaaccgct taagcatcgt gcagcgaagc cgcgtgcaaa gtgttttcca tcaatgtggc
      481 aatcgtcatc ggacccacgc cgccgggaac gggcgtaatc atcgccgccc gttcttttgc
      541 cgtttcaaat tccacgtcgc cgcacaggct gccatcgtcc aaacggttga tgcccacatc
      601 aataacgacc gcgccaggtt tgatccattc gcctttgaca aagttcggaa tgcctacgcc
      661 gaccaccaaa atatcggctc cggcaacctc gtctgtcaga ttttcggttg cgctgtggca
      721 gaccgttacc gttgcgcgcg ccagcagcag ttccaaagcc tgcgggcggc cgacgatatt
      781 cgacgcgccg accacgaccg cttttttccc cttcggatca atgccgtaag cttccaaaag
      841 cgtcatcacg cccttgggcg tacacgggcg catcagcggc attttgaccg ccagcctgcc
      901 gacattgtaa ggatggaagc cgtccacgtc cttatccggc gaaatacgtt ccaaaaccgc
      961 ctggctgtcg aggtgcttcg gcagcggtag ctgaaccaga ataccgtcca cttcggaatc
     1021 ggcattcagg cggtcgacca gtgccagcag ttcttcctgc gatgttgatt cgggcagctc
     1081 gtaagacagt gatttgatgc cgcatttttg gcaggcagtt ttcttgttgc ggacataaac
     1141 cgcgctggca gggtcgcctc cgaccaaaac cacggccagg caaggcatgt gcagattgtc
     1201 ctgttggcgt tgcgccaccg cttcggcaac cgcctgcagg cgtttttgcg aaacttcttt
     1261 accattgatc agttgtgccg acatatccgt ccttttgtta aacaggtaga aaatgctgaa
     1321 aattatcgca ttttccgttt gtttttacag caggatgccg aaaataaatt tgccctgaaa
     1381 taaaatacgg ctgtttggac ggattaaata ttttttcaaa tcaaaaaact atatttttca
     1441 tcttggtgtc ttgaaaataa atgttgacgc atctttccct gagttgtata gttcgctact
     1501 tcaagtcggg gcgtagcgca gcccggttag cgcatctgct ttgggagcag agggtcgtga
     1561 gttcgaatcc caccgccccg accaatcaat cagcacgaat cggcatgagc gcccgtagct
     1621 caaccggata gagcaccgac cttctaagtc gggggttaca ggttcgattc ctgtcgggcg
     1681 tgccaagaat ttgattgaga accgttttta tggtggctgt agctcagttg gtagagcccc
     1741 ggattgtgat tccggttgtc gtgggttcga gccccatcag ccaccccaga taaaggcccg
     1801 tacttttcgg tacgggtctt tttccgtttg cacgtactgt ccgcaccgtc gtatcggtgc
     1861 ggcggtatgc catctgaaag ctgggccggt catacggtaa ccgcttcaga cggcatttgc
     1921 gccttcggct ttgcctttcg catatgcttc tgtatcatgt gggtttttct gtcttttatg
     1981 gtttttgtcg gagactttga tggttgacgc ttggtctgtc cctgattttg aatcgaaaat
     2041 ctgtccgccc gaggcgttgg cggcgcgttt ggcgttgttg ccgcgcccgc tggtgtttac
     2101 caacggctgt ttcgacatcc tccacagggg gcacgttact tatctggcgc aggcgcgttc
     2161 gatggggggc gcgttggtgc tggcgttgaa taccgatgct tcggtgcggc gtttgggcaa
     2221 gggcggcgac cgcccgatta atcctttgga gaaccgtgcc gccgttgccg ccgctttgga
     2281 aagtgtggat ttggtaacgt ggtttgacgg ggatacgccg gcggcgttga ttgaggcggt
     2341 caaacctgag attttggtca agggcggcga ttgggctgcg gataagattg tcggtgcggc
     2401 agaaacgttg gcgcgcggcg gtcaggtgtt ttcaattccg tttctgcacc agacttcgac
     2461 aacgaagact ttggcaaaaa tccgtgcggc agagggcgga aaatgacggt tttgaagcct
     2521 tcgcactggc gggtgttggc ggagcttgcc gacggtttgc cgcaacacgt ctcgcaactg
     2581 gcgcgtatgg cggatatgaa gccgcagcag ctcaacggtt tttggcagca gatgccggcg
     2641 cacatacgcg ggctgttgcg ccaacacgac ggctattggc ggctggtgcg cccattggcg
     2701 gttttcgatg ccgaaggttt gcgcgagctg ggggaaaggt cgggttttca gacggcattg
     2761 aagcacgagt gcgcgtccag caacgacgag atactggaat tggcgcggat tgcgccggac
     2821 aaggcgcaca aaaccatatg tgtgacccac ctgcaaagta agggcagggg gcggcagggg
     2881 cggaagtggt cgcaccgttt gggcgagtgt ctgatgttca gttttggctg ggtgtttgac
     2941 cggccgcagt atgagttggg ttcgctgtcg cctgttgcgg cagtggcgtg ccggcgcgcc
     3001 ttgtcgcgtt taggtttgga tgtgcagatt aagtggccca atgatttggt tgtcggacgc
     3061 gacaaattgg gcggcattct gattgaaacg gtcaggacgg gcggcaaaac ggttgccgtg
     3121 gtcggtatcg gcatcaattt tgtcctgccc aaggaagtag aaaatgccgc ttccgtgcaa
     3181 tcgctgtttc agacggcatc gcggcggggc aatgccgatg ccgccgtgct gctggaaacg
     3241 ctgttggtgg aactggacgc ggtgttgttg caatatgcgc gggacggatt tgcgcctttt
     3301 gtggcggaat atcaggctgc caaccgcgac cacggcaagg cggtattgct gttgcgcgac
     3361 ggcgaaaccg tgttcgaagg cacggttaaa ggcgtggacg gacaaggcgt tttgcacttg
     3421 gaaacggcag agggcaaaca gacggtcgtc agcggcgaaa tcagcctgcg gtccgacgac
     3481 aggccggttt ccgtgccgaa gcggcgggat tcggaacgtt ttctgctgtt ggacggcggc
     3541 aacagccggc tcaagtgggc gtgggtggaa aacggcacgt tcgcaaccgt cggtagcgcg
     3601 ccgtaccgcg atttgtcgcc tttgggcgcg gagtgggcgg aaaaggcgga tggaaatgtc
     3661 cgcatcgtcg gttgcgccgt gtgcggagaa ttcaaaaagg cacaagtgca ggaacagctc
     3721 gcccgaaaaa tcgagtggct gccgtcttcc gcacaggctt tgggcatacg caaccactac
     3781 cgccaccccg aagaacacgg ttccgaccgc tggttcaacg ccttgggcag ccgccgcttc
     3841 agccgcaacg cctgcgtcgt cgtcagttgc ggcacggcgg taacggttga cgcgctcacc
     3901 gatgacggac attatctagg gggaaccatc atgcccggtt tccacctgat gaaagaatcg
     3961 ctcgccgtcc gaaccgccaa cctcaaccgg cacgccggta agcgttatcc tttcccgacc
     4021 acaacgggca atgccgtcgc cagcggcatg atggatgcgg tttgcggctc ggttatgatg
     4081 atgcacgggc gtttgaaaga aaaaaccggg gcgggcaagc ctgtcgatgt catcattacc
     4141 ggcggcggcg cggcaaaagt tgccgaagcc ctgccgcctg catttttggc ggaaaatacc
     4201 gtgcgcgtgg cggacaacct cgtcattcac gggctgctga acctgattgc cgccgaaggc
     4261 ggggaatcgg aacatactta aaaaatacac cctttttcaa cacggggcgg catccgtccg
     4321 aatccgtccg gattctgccg cccgacagac tgccgcccag gcgggcgggg tttcaaacat
     4381 tgaggaaatt ctaatgaaat ggctcttcac ccttttggcg gtactcaata ttgccgtatt
     4441 cggcggcacg gtgggctata aattggccgt taaagcgacg ggcggcgtgc cggaaaaccg
     4501 ggccgtcgaa aacacgcccc ccgcaacgcc cgccgccgac aatgcggcgg catccgtgga
     4561 ggatacggcg gcactgctca aaccgggcga catcctgacc gaagagcagg ccgagcagtt
     4621 gcgcttgaaa aaggaagcgg aacagaaaaa actgagggag aaaaaacagc gtgaagaaaa
     4681 agcccgccgc gaaaaactcg ccgcagaaaa ggcgcaggcg gaacgcgaaa acggcgcggc
     4741 ggatgcctta tgcgccgcgc aggcaagcct cacgatggac gaagatgact accaccgcat
     4801 caaaggactt ttgggcaaat ggtcgcacgt tgccagcagg agcgtcgaaa aacgcaccgc
     4861 caaagccaaa cctgccgaca aaacctaccg cgtcgtcctg cccgtttccg ccgatgccga
     4921 aaatcaggcg gcggagctgt ctgccaaagg tttcaacccc ataccgtttg acggagcatt
     4981 gagtttgggt gtcggcaaca gccgggaaaa cgcccaagcc ctgcaaaacc ggcttgccgg
     5041 cgccggattc ggcggggcgc atattgtcga acactttgcc gaagccgaca ggcaggacga
     5101 ttctttgagc gtgtcgcgta tgacggtttt gtttaccggc gtgaatgccg ccgatgcgga
     5161 cgaaatccgt aaaatcacgt ccctatacgg caaactgaac ctcaagtctt gcaaataggc
     5221 gcgaaagccg gacgataaac gccaaaccca ccgcgaaggt gggtttggcg tttcggtttt
     5281 gagtatctga tatataaaaa gggaatgtat gtctcgtata tgccgactcc aagtgtgaaa
     5341 gtgatgatgg ggaaatatgg tgtttaatgg ttcggaacgg acggcttccg agtggttttg
     5401 attatacgct aataataata attatcaata aaaatttaaa aataaatgca gattttttgt
     5461 aatacgatga aaaataaaca aattaacctg tccgccgatg tttgtgattc gggtcggcaa
     5521 actttcattg tgtcggacaa atcggggtcg gcagtcatag ctgagggctt cgtgcctgac
     5581 ggaaataagg ttgcccgtgt ttgaacatcc gtccgattgg gcgcggacag ggttgtcatt
     5641 cgggggtgtt tgagcgtgga ttcggggatg cgaaagcggt aaaaccgttg cggatgaagg
     5701 cttacggacg gctttaggta tttttaagtg tattcaaaca acaacaaaac gcttcttcta
     5761 ttgccgtatt tagggcttgc gcggcagcct gcacaccgtg gaggccgagt gtttcgttgg
     5821 ttgcggcatc gggacgggcg gcaaggtttt ccggccggtt tttccatttt ttccgacaaa
     5881 gccgtgatgc cggggaatgg cggtttgtcc gtttcggacc gccgccgttt tgagccgcga
     5941 tgccgtctga agccttcaga cggcattttt gccgcctttt tcaatattcc gccgaatgcc
     6001 aaaacggctg tccgactgtc ggcgtgctgg cttgcgcttt gtcgcgtgct tcgaccggtc
     6061 cggcttcaaa tgccagccgt ccggattcga cggcgagtgc gaaggcgcgt gccatattga
     6121 ctggatcgcc gctgcgggaa acggcggtgt tcaaaagcac gccgtcaaag ccccattcca
     6181 tcacttgtgc cgcctgtgag ggcaaaccca agcccgcgtc gataatcagc ggcgtgtagg
     6241 gcaggcgttc gcgcaggacg ttcaacgcgt aggcgtgaac cgcgcccaaa cccgtgccga
     6301 tcggggccgc ccacggcatc aacgcctgac agcccacatc gagcaggcgg cggcaggcaa
     6361 tcaggtcttc ggtgcaataa ggcagcactt tgaagccgtc tttaatcagg atttccgccg
     6421 cttcgacaag ttggaacaca tccggctgca aggtgtcgtc gtcgccgatg agttcgagtt
     6481 taatccaatc ggtttcaaac acttcgcgcg ccatttgcgc cgtcgttacc gcttcctgca
     6541 cgctttggca gcctgccgtg ttcggcagga cgggaacgcc ggtttcttga agcagcgacc
     6601 aaaacccctg accgtgtgcc tccccaccgc ttcccgcgcg gcgcagcgag acggtaatca
     6661 tcgcaggccg ggcggttcgg acggattgtt tgaggatttc aggggtcggg taggcagccg
     6721 tgccgagcag cagccgcgag gggaaagttt ctccgtatag ggtgagcata atgggttcct
     6781 tt
//
LOCUS       DAWHRS010000117         6633 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWHRS010000117 DAWHRS010000000
VERSION     DAWHRS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.117"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1005
                     /locus_tag="WIB82_001644"
     CDS             <1..1005
                     /locus_tag="WIB82_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3842918.1"
                     /translation="PHVPVSIPTATPLTGEVKLSSDNSKIENINTANTETHPPRRTRR
                     SLYASPQNTSSGISIQQREVEKIYFGVKSPEKSFIFQTPGGAQYALSSYADPIVPSYS
                     SPDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIGPNGEIDLFA
                     GGFPVGKTPPPAFSYGNSTPETALSKGKITYQVWGIRVKDGQFVTSSYTPPKGSSFTG
                     YTNTPVLSFITANFNSNKLAGEIRGNSDYGPSVKIENATISGPSFSGNATSGGKTGNL
                     EGKFFGKFNGSYGNTETSIGGKITFKDDRSLDTVFGGVSYVKKLDETANRDTEHLTKQ
                     "
     gene            1033..3459
                     /gene="hupB"
                     /locus_tag="WIB82_001645"
     CDS             1033..3459
                     /gene="hupB"
                     /locus_tag="WIB82_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ3842919.1"
                     /translation="MSIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVKIENKGYVFSPTQSFSKYQTYVATGVARSQPDPQEWVNKSTLFKLGY
                     NFNDRNRIGWIFEDSRTDRFTNELSNLWTGTIYSPATGDYRHRQDVSYRRRSGVEYKN
                     ELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINSEVYHSFRHIRQNTAQWTAD
                     FEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFVKLYDPKIFTSNQAKITMLIESRSK
                     YKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHAG
                     FSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAETAKNF
                     ELGLAGSGKAGNFKFSGFQTRYRNFIELTYMGVSSDKPNSPNYAPISDGTALVSSPVW
                     QNQNRSSAWVKGLEFNGTWNLDSIGLPQGTHAGVNVSYIKGKAKQTNGQETPINALSP
                     WSAVYNLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFDL
                     SAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPGR
                     SYNFTIEAKF"
     gene            complement(4067..5701)
                     /gene="groL"
                     /locus_tag="WIB82_001646"
     CDS             complement(4067..5701)
                     /gene="groL"
                     /locus_tag="WIB82_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ3842920.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAIESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(5795..6082)
                     /locus_tag="WIB82_001647"
     CDS             complement(5795..6082)
                     /locus_tag="WIB82_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ3842921.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVE"
     gene            6311..>6633
                     /locus_tag="WIB82_001648"
     CDS             6311..>6633
                     /locus_tag="WIB82_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3842922.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEDPVEPDESDFGGWRKGRCGRGAAGKVVVFDILKRNGRV
                     YTVVV"
BASE COUNT         1612 a         1932 c         1599 g         1490 t
ORIGIN      
        1 ccgcacgtcc ccgtgtccat ccccaccgcc acgccgctga ccggtgaggt aaagttatca
       61 agcgataaca gcaaaatcga aaacatcaac accgccaaca ctgaaacaca tccgccgcgc
      121 cgcaccagac gctccctcta tgcctccccg caaaacacat cctccggtat ttccattcag
      181 cagcgggaag tagaaaaaat ctatttcggt gttaaatcac cagaaaaatc attcatcttc
      241 caaacacccg gcggcgcgca atacgccctt tcatcctacg ccgaccccat cgtccccagc
      301 tactcctccc ccgatttcaa aattcccgac cgccacgcag ggcagcggct tgccgacggc
      361 agccgcatct ttatctgctg cagcgactcc ggggctactt cgtatgcgga aattaccaaa
      421 caagattata tgaaattcgg ggcgtggata ggtcccaacg gcgaaatcga cctcttcgcc
      481 ggcggcttcc ccgtcggcaa aacgccacct cctgcgttct cctacggcaa cagcacgccc
      541 gaaaccgcat tgtcgaaggg caaaatcact tatcaggttt ggggtatccg cgtcaaagac
      601 gggcaatttg ttacctcctc ttatacgccg cccaaaggca gctcttttac cggctacacc
      661 aacacccccg tcctttcctt tatcaccgcc aatttcaaca gcaacaaact ggcaggcgaa
      721 atccgcggca acagcgatta cggaccgagt gtgaaaattg aaaacgcaac gatcagcggt
      781 ccgagctttt ccggcaatgc cacctccggc ggcaaaaccg gcaacttgga aggcaagttt
      841 tttggaaaat tcaacggcag ctacggcaac actgaaacca gcataggcgg caaaatcact
      901 tttaaagacg accgctccct cgataccgta ttcggcggtg tgagttatgt aaaaaaactt
      961 gatgaaaccg ccaatcgaga taccgaacac cttactaaac aataaaccat ctcatcccta
     1021 caaaggtttc ttatgtccat ccccttcaaa cccgtattgg ctgccgccgc catcgcccaa
     1081 gcgtttcccg cctttgcggc agaccccgcg ccgcagtccg cccaaacgct gaacgaaatc
     1141 accgttaccg gcacgcacaa aacccaaaaa ctcggcgaag aaaaaatccg ccgcaaaacc
     1201 ttagacaagc tcttggtcaa cgacgaacac gacctggtgc gctacgaccc cggcatttcc
     1261 gtcgtcgaag gcggcagggc gggttctaac ggctttacca tacgcggcgt ggacaaagac
     1321 cgcgtcgcca tcaacgttga cgggctggcg caggcggaaa gccgctcttc cgaagccttc
     1381 caagaattgt tcggcgcgta cggcaacttc aacgccaacc gcaacacttc cgagccggaa
     1441 aacttttccg aagtaaccat caccaaaggc gcggactcgc tcaaatccgg cagcggcgca
     1501 ttgggcggcg cagtcaatta ccaaaccaaa tccgcaagcg attatgtttc cgaagacaag
     1561 ccctaccatt tggggataaa gggcggcagc gtcggcaaaa acagccaaaa gttcagcagc
     1621 atcaccgccg ccggcaggct ctttggtttg gatgccttat tggtttatac ccgccgcttc
     1681 ggcaaagaaa ccaaaaaccg ctcgaccgag ggcgatgtca aaattgaaaa caagggatat
     1741 gttttcagcc caacccaatc attcagcaag taccagacct acgtagccac aggggttgcg
     1801 cgctcccaac ccgacccgca agaatgggta aacaaaagca ccttgttcaa gctgggctac
     1861 aatttcaacg accgcaaccg catcggctgg atttttgaag actcccgcac cgaccgtttt
     1921 accaacgagc tgtctaattt gtggacgggt acaatttact ctcccgcaac gggcgactac
     1981 cgccaccgcc aagacgtgag ctaccgccgc cgctccggcg tcgaatacaa aaacgaattg
     2041 gaacacggcc cgtgggacag cctcaagctg cgctacgaca agcagcgcat cgatatgaac
     2101 acttggactt gggacatccc gaaaaattac gatttgcgcg gcatcaacag tgaagtttac
     2161 cattcgttcc ggcatatccg ccaaaacacc gcgcaatgga ctgccgattt tgaaaaacaa
     2221 ctcgactttt ccaaagccgt ttgggcggcg caatacggct tgggcggcgg cagaggggac
     2281 aatgccaact cggattacag ctatttcgta aaactgtacg accccaaaat cttcacttcc
     2341 aaccaagcca aaatcacgat gctgatcgaa agccggtcga aatataaatt tgcctattgg
     2401 aacaatgcgt ttcacttggg cggcaacgac cgcttccgcc tgaatgcggg catacgctac
     2461 gacaaaaaca gcagcagcgc gaaagacgat ccgaaataca ccaccgccat ccgggggcag
     2521 attccccatt tgggttcgga acgcgcgcac gcgggcttca gctacggcac ggggttcgac
     2581 tggcggttta ccaagcatct gcacttgttg gcaaaataca gcaccggctt ccgcgcaccg
     2641 acttcggacg aaacctggct attgttcccg catcctgact tttaccttaa agcaaacccc
     2701 aacctgaagg cagaaaccgc caaaaacttc gagctgggac ttgccggcag cggcaaagca
     2761 ggaaacttta aattctccgg tttccaaacc cgctaccgta attttatcga actgacctac
     2821 atgggcgtat cctcagacaa gcctaacagc cctaattatg cgccgatttc cgacggtact
     2881 gcattggtca gctcgcccgt ttggcaaaac caaaaccgct cctcggcatg ggtgaaaggt
     2941 ttggaattta acggcacatg gaacctcgac agcatcggtt tgccgcaagg cacgcacgca
     3001 ggtgtcaatg tcagctacat caaaggcaag gccaaacaaa ccaacggaca ggaaacccct
     3061 attaacgccc tttcgccatg gagtgcggtt tacaatctgg gctacgacgc tccttccaaa
     3121 cgttggggca tcaacgccta cgccacgcgc accgccgcca aaaagccgtc cgacaccgtc
     3181 cacagcaacg acgacttgaa caacccgtgg ccttatgcca aacacagcaa ggcctatacg
     3241 ctgttcgacc tttccgccta cctcaacatc ggcaaacagg ttacgctccg cgccgccgcg
     3301 tacaacatta ccaacaagca gtactacact tgggaatctt tacgcagcat ccgcgagttc
     3361 ggcacggtca accgcgttga caacaaaacc cacgccggca tccaacgctt tacctcgccg
     3421 ggcaggagct acaatttcac catcgaagcg aagttctaag ctcctgatgt gccaatgccg
     3481 tctgaaaccc gatgatttgg gtttcagacg gcatttttta cggtcgcacc gccgccccct
     3541 ttcaggcttg taccaagccc aatcccgcaa ctctccgtca ttcccgcaac tctccgtcat
     3601 tcccgcgact ctccgtcatt cccacggaag tgggaatcta gaaacgcaaa gctgcaagaa
     3661 tttatcggaa atgactgaaa ctcaacgaac ctggattccc gcctgcgcgg gaatgacgaa
     3721 gctatccgca cagaaacctg caccacgtca ttcccacgaa agtgggaatc cagaacgtaa
     3781 aatctgaaga aaccgtttta tccgatacgt ttccgcaccg acagacctag attcccgctt
     3841 tcgcgggaat gacggcggaa aggttgctgt ttttccgata aattcctgcc gctcttcgtt
     3901 tttgggatgg cgggaaataa aacaaaagcg cgcgtatcaa aaaacaaaaa tgcaaagaac
     3961 ggcgttggca attttaaggt ttgcgcctta gccgcactta ttcgcaaaaa aaaccgcacg
     4021 gcgttgaccg tgcggcttgt tgtctgaaag cttcagacgg catttcttac atcatgccgc
     4081 ccataccgcc catgccgccc atgtcaggca tagcaggttt ttcttcaggg atttcagcga
     4141 tcatgcagtc tgtggtcagc atcagaccgg cgatagatgc ggcgtgttgc agcgcggaac
     4201 gggttacttt ggcaggatcg agtacgccca tttcaatcat atcgccgtat tcgccgctgc
     4261 cagcgttgta accgtagttg cctttgcctt ccaacacttt gttcacgact acgctgggtt
     4321 cgccgcctgc gttggcaacg atttggcgca gcggagactc aatggcgcgc aagacgattt
     4381 gtacgcctgc gtcttgatcg gcattgccgg tgtgcaggtt ttccagagcg gcacgggcgc
     4441 gcaacagggc tacgccgccg cctgcaacca cgccttcttc aacggctgcg cgggtagcat
     4501 gcagcgcgtc ttctacgcgg tcttttttct ctttcatttc gacttcggta gcggcaccga
     4561 ctttgattac tgccacgccg cctgccagtt tggccacgcg ctcttgcagt ttttctttgt
     4621 cgtaatcgct ggttgcggtt tcgatttgtt ggcggatttc ggcaacacgc gcttcgattt
     4681 gggctgcgtc gccgaagccg tcgatgatgg tggtgttttc tttaccgatt tcgatgcgtt
     4741 tggcttgacc caagtcgtcc aaagtcgctt tttccagaga caggccgact tcttcggaaa
     4801 tcaccgtgcc gccggtcagg atggcgatgt cttgcagcat cgctttgcgg cggtcgccaa
     4861 agcccggagc tttaacggca acggttttca ggatgccgcg aatgttgttt accaccaaag
     4921 tcgccaaggc ttcgccttct acgtcttcag cgataatcaa gagcggacgg ctggcttttg
     4981 ccacttgttc caaaacaggc aacaagtcgc ggatgttgct gatttttttg tcgaacagca
     5041 atacaaacgg attgtccaag ccggcgattt gtttttccgc atcgttgatg aagtaaggag
     5101 acaggtagcc gcggtcgaat tgcatacctt caaccacgtc cagctcgttt tccaaagatt
     5161 tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct tcggcaataa
     5221 tcgcgccgac ttgttcgtcg gagttggcgg aaatcgagcc gacttgggcg atttctttag
     5281 aagtgtcgca aggtttggcg atgtttttca gctcttcaac caaagcggcg acggctttgt
     5341 cgataccgcg tttcaggtcg gtcgggttca taccggcggt aacgtatttc ataccttcgg
     5401 caacgatgga ttgcgccagt acggtggcgg tagtcgtacc gtcgcccgcc acgtcgttgg
     5461 ttttggacgc gacttctttt accatttgcg cgcccatatt ttcaaacttg tctttcagtt
     5521 cgatttcttt agcgacggtt acgccgtctt tggtaatgtg cgggccgccg aaagcgcggt
     5581 caaccaccac gttgcggcct ttgggaccca gggttacgcg cacggcgttt gccagaatgt
     5641 tcacgccgtt taccattttt tgacggactt cattgccgaa ttgtacgtct tttgctgcca
     5701 tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc gtctgaagac
     5761 ggtttgccgt ttcagacggc atcatcattc gcacttattc aacgatgccg aaaatatctt
     5821 cttcgcgcat taccaacagc tcttcgccgt cggcttttac ggtttggccg ctgtatttgc
     5881 cgaaaatgat tttgtcgccg actttgacat ccagcggacg gcgcgcgccg tctttaccga
     5941 ttttgcccgc gcccacggcg atgacttcgc ccatatcggg tttttcggcg gccgcacccg
     6001 gcaaaacgat gcccgatgcg gttttctctt cagcttccaa gcgtttgacg acgacgcggt
     6061 cgtgtaaagg acggatggtc atatctatgc tccgataaaa tggtttgaaa acaatcatct
     6121 gcccgaacgg ttcggacaga ttaaggatga aaaccggaca gccgtcaggc ggctgcccgt
     6181 ataagtcggc aaaattaggg tgtgtgcggg caaattcaag tgaggcggaa aaaatttatt
     6241 tccgccgttt tttatagtgg actaaattta aggggctgta ctagattagc agatatgtta
     6301 ccctcgaaat atgaagataa cgcactgcaa attaaagaaa aaagtacaga aagagctgct
     6361 ccgttttttt gtactcgaag ttaccgcccg ttctgccgcc gatattttgg gtatccatcc
     6421 caattcggca gcactgttct accgtaaaat ccgcacggtt atcaaccatc atttggccct
     6481 ggctgccgat gaggtttttg aggatcctgt cgagccggac gaaagcgatt tcggcgggtg
     6541 gcgtaaaggc agatgtggtc gtggtgcggc aggaaaagtg gttgtcttcg acattctgaa
     6601 acgcaacgga cgggtctata ccgttgtggt gga
//
LOCUS       DAWHRS010000118         6629 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWHRS010000118 DAWHRS010000000
VERSION     DAWHRS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.118"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(58..1851)
                     /locus_tag="WIB82_001649"
     CDS             complement(58..1851)
                     /locus_tag="WIB82_001649"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ3842923.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKLKAQGSGTI"
     gene            2096..3205
                     /gene="proB"
                     /locus_tag="WIB82_001650"
     CDS             2096..3205
                     /gene="proB"
                     /locus_tag="WIB82_001650"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ3842924.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3218..4480
                     /locus_tag="WIB82_001651"
     CDS             3218..4480
                     /locus_tag="WIB82_001651"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ3842925.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDEKRIGAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(4705..>6629)
                     /locus_tag="WIB82_001652"
     CDS             complement(4705..>6629)
                     /locus_tag="WIB82_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ3842926.1"
                     /translation="VYDEAADIYIEEPAAPDAWVVEPPEVPKVPMPAIDIPPPPPVSE
                     IYNRTYEPPSGFEQAQRSRIAETDHLADDVLNGGWQEETAAIADDGSEGAAERSNGQY
                     LSETEAFGHDSQAICPFEDVPSERPSCRVSDTEADEGAFQSEETDAVYEHLPTTDLLL
                     PPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVVDSYSGPVITRYEIEPDVGVRGN
                     SVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPNPKRQMIRLSEIFNSPEFAESKS
                     KLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKSVGVNAMILSMLFKAAPEDVRMI
                     MIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWCVNEMEKRYRLMSFMGVRNLAGF
                     NQKIAEAAARGEKIGNPFSLTPDNPEPLEKLPFIVVVVDEFADLMMTAGKKIEELIAR
                     LAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAFQVSSKIDSRTILDQMGAENL
                     LGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYLKQFGEPDYVDDILSGGGSEELP
                     GIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRALRIGYNRAARLIDQMEAEGIVSA
                     PEHNGNRTILVPLDNA"
BASE COUNT         1469 a         1828 c         1828 g         1504 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcagttt ggcggcacta aattccagtt tgctcaaggc
      121 ggaaaggtag gctttggcgg tagcgaccaa aacgtcggta tccgcgccct gaccgttgac
      181 aacgcggttg ccgcgcgcca gacggacgct ggtttcgccc tggctttccg taccttgcgt
      241 gacggcgttg acggaataaa tctgcaaagc cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatc gcatccacgg ggccggagcc tgtggcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga
      421 gatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc
      661 ggacataatc tcgtaagttt cgcggtgttt cagcacccca tcctgatgga tgcccgattc
      721 atgcgaaaag gcattggcac cgacaatggc tttattgggc tgcacgggat agcccgtaat
      781 agtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgc gcgccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttgg caatcagttc gcggaaaaat tcttcggttt tatacgggat
     1081 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa tcaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 gcgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgactgctc
     1561 gccgtcgcgc agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgcttc
     1681 cgccaacgcg gtcagctttt gcagttgcgc cagaggcagc gagccgcgcg tgcgccattt
     1741 atagatagcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaatctcaat aagccgccat agattttatt aattttatac
     1981 attttgtcca ttttattgca gacgcggcgt gattgttgac aattgcggca gaacagggca
     2041 aattcggcgg cacaaggtgg caacgagaca ggacaaacca acaaggacgg aaacaatgaa
     2101 atacaaaaga atcgtattta aagtcggcac atcttcgatt acccattcgg acggcagtct
     2161 ctcgcgcggc aaaatccaaa ccatcacctg ccagcttgcc gcattgcatc atgcgggaca
     2221 cgagctggtc ttggtgtctt ccggcgcggt tgcggcaggg ttcggtgcgc tgggtttcaa
     2281 aaaacgtccg gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct
     2341 gatggaagaa tatacggcaa acctgtcttc agacggcatc gtgtccgcgc aaatcctgct
     2401 cagccgcgcc gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt
     2461 gctgctgcaa cgccgcgccg tccccatcat caatgaaaac gatacggttt cggttgagga
     2521 attgaaaatc ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga
     2581 cctcttggtg ctgctgaccg acatagacgg tctttacacc ggcaacccga acagcaatcc
     2641 cgatgccgta cggctggaca aaatcgaaca catcaaccat gaaatcatcg aaatggcggg
     2701 cggctcgggt tcggcaaacg gcacaggcgg tatgctgact aaaatcaaag cggcgacgat
     2761 tgcgaccgag tccggcgtac cggtctatat ctgttcctcg ctcaaacccg atgcacttgc
     2821 cgaagcggca gataatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg
     2881 gacgcagaag caatggctgg cgttctattc cgaaagcagg ggcggcgttt atgtggacga
     2941 aggtgcggaa cacgctttgt ccgaacaggg aaaaagcctg ctgatgtcgg gcattgccgg
     3001 aatcgaaggg catttttccc gtatggacac cgtaaccgtg tacagcaagg caaccaaaca
     3061 gcccctaggc aaagggcgcg tcctgttcgg ttctgccgcc gccgaagacc tgctcaaatc
     3121 gcgtaaggcg aaaggcgtgt tcatccatcg ggacgactgg atttccatca cgcccgaaat
     3181 acgcctgctt ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct
     3241 tgcccttgcc aaagcggcaa aaaaatccgt caacacggcg gatacggaag aaaaaaaccg
     3301 cgcgctgctt gctatggcgg acagcttgga agcggcggcg gcagatattt tggcggcaaa
     3361 ccgtcaagac ttggaagccg cggcaggcaa cattcccgaa agcatgaccg accgcctttt
     3421 gttggacgaa aaacgcattg gcgcgatggc ggacggcatc agggcggttg ccgcgctgcc
     3481 cgaccccgtg ggcgaaatac tggaaacctc gactttgccc aacggcttgg aaatcgtcaa
     3541 aaagcgcgtg gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacttc
     3601 cgatgcggcg gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga
     3661 tgcattccaa tccgcacgcg ccatcgttgc cgccctgaaa acggggttgg cacaaacccg
     3721 catcgacccc gacgcgctcc agttgattga agacaccggg cgcgaaagca gttacgaaat
     3781 gatgagggcg aaagattatc tagatctgct gattccgcgc ggcggggcgg ggctgatacg
     3841 ggcggtggtt gagaatgccg tcgtgccggt cattgaaacg ggaacgggca ttgtccacat
     3901 ttatatcgac aaggacgctg attgggataa ggcactccgt atcgtgtaca acgccaaaac
     3961 cagccgtccg tccgtgtgca actcgatgga agtgctgctg gtgcatgaag acattgctgc
     4021 cgacttcctg cccaagctcg aacgactgtt ggttcgcgac cgcatagaag ccggactgcc
     4081 gcccgtccgc ttccgtttgg atccgcaggc ggcgcggcat atcggcggcg aagcggcggg
     4141 tgcagacgat ttcgataccg agtttttaga ctacatcctc gccgtgaaaa ccgtcgcatc
     4201 ggtcgaagag gcggtcgggc acatcgaagc ccacggcaca caccattcag acggcatcgt
     4261 taccgaaaac cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta
     4321 tgtcaacgcg tccacgcgct ttaccgacgg cggcgaattc ggcttgggtt gcgaaatggg
     4381 catctccacg caaaaactcc acgcccgggg cccgatgggt ttgaaagagc tgacgagtta
     4441 caaatacatc gtacaaggca cgggacaggt cagggaataa tccgaattca aatgtctgat
     4501 atagtggatt aactttaaac cggtacggcg ttgcctcgcc ttagctcaaa gagaacgatt
     4561 ctctaaggtg ctgaggcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt
     4621 cgtcgccttg tcctgatttt tgttaatcca ctataaaaat gccgtctgaa acagtcttca
     4681 gacggcattt ccatttgcaa aaaatcaagc attgtccaag gggacgagaa tcgtacggtt
     4741 gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc atctggtcaa tcagacgcgc
     4801 ggcgcggttg tagccgatac gcaaggcgcg ctgtacgccc gaaatgctgg ctttgcgcgt
     4861 tttcaggaca acggatacgg cctcgtcgta catcggatcg gtttcgtcgt cgccgctgcg
     4921 cccgatgccg ggcagctctt cgctgccgcc gccgctcaaa atatcgtcaa catagtccgg
     4981 ttcgccaaac tgcttcagat attcgaccac gcggtgcacc tcttcatccg aggcaaacgc
     5041 gccgtgaacg cgctgcggat aggccgtacc cggcggcagg aacagcatat cgccctgacc
     5101 gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg ctgtcgattt tgctggatac
     5161 ttgaaacgcg atacgcgtcg ggatgtttgc cttaatcaga cccgtgatga catcgacgct
     5221 ggggcgttgc gtggcaagaa tcaaatggat gcctgccgcg cgggcttttt gggcgaggcg
     5281 ggcaatcagt tcttcgattt tcttgcctgc cgtcatcatc aggtcggcaa actcatcaac
     5341 cacgaccacg ataaacggca atttttccaa aggttcggga ttgtcgggcg tgaggctgaa
     5401 cgggttgccg attttctccc cccttgctgc ggcttcggcg attttttgat tgaaacccgc
     5461 aagattgcgc acgcccataa agctcatcag gcggtagcgt ttttccattt cgttaacaca
     5521 ccagttcagc gcgtttgccg ccagcttcat atcggtaacg acaggggcga gcaggtgcgg
     5581 gatgccttcg taaatgctca attccagcat tttcggatcg atcataatca tacgcacgtc
     5641 ttccggcgcg gctttgaaaa gcatagacag aatcatcgcg ttgacaccca ccgatttgcc
     5701 cgaaccggtc gtaccggcaa ccaataaatg cggtgctttg cccaagtcgg ttacgacggg
     5761 ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg gatttggatt cggcaaactc
     5821 gggcgaattg aagatttcgc tcaggcgtat catttggcgt ttcgggttcg gaagttccaa
     5881 acccatacag gttttgccgg ggatggtttc gacaacgcgg atggaagcca cgccgagcga
     5941 acgcgccaag tctttttcca gatttagaac ggaattgccg cgcacgccga catcgggttc
     6001 gatttcataa cgcgtaatca cggggccgga ataagaatcg acaaccttga ccttgacttt
     6061 gaactccgcc aatttttctt cgatggtgat gctgttttcc aacagttctt cttcggtttg
     6121 cgtcgcctcg ggattgaaca gcggaggcag aagcaggtcg gttgtcggca ggtgttcgta
     6181 taccgcatcg gtttcttcag attggaacgc cccttcatcc gcttccgtat ccgatacccg
     6241 gcaggacggg cgttcagacg gcacatcttc aaacggacaa atcgcctgac tgtcatgccc
     6301 gaacgcttcg gtttccgaca gatattgccc gtttgaccgc tctgccgcac cttcactgcc
     6361 gtcatctgca atagcggcgg tttcctcctg ccaacctcca ttcaaaacat catcagcaag
     6421 atggtcggtt tcagcaatgc ggctgcgttg cgcctgctcg aatcctgacg gcggttcata
     6481 ggtacggttg tagatttccg atacgggagg cggcggcgga atatcgattg cgggcatggg
     6541 aactttcggc acttcgggtg gttcgaccac ccaagcatcg ggcgcagcag gttcttcaat
     6601 atagatatcg gcagcttcat cataaaccg
//
LOCUS       DAWHRS010000119         6389 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWHRS010000119 DAWHRS010000000
VERSION     DAWHRS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.119"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1041)
                     /gene="murA"
                     /locus_tag="WIB82_001653"
     CDS             complement(<1..1041)
                     /gene="murA"
                     /locus_tag="WIB82_001653"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ3842927.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTE"
     gene            complement(1261..1336)
                     /locus_tag="WIB82_001654"
     tRNA            complement(1261..1336)
                     /locus_tag="WIB82_001654"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1301..1303),aa:Lys,seq:ttt)
     gene            complement(1405..2379)
                     /locus_tag="WIB82_001655"
     CDS             complement(1405..2379)
                     /locus_tag="WIB82_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ3842928.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEGDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLSDVEERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            complement(2412..2861)
                     /locus_tag="WIB82_001656"
     CDS             complement(2412..2861)
                     /locus_tag="WIB82_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ3842929.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VENAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            complement(2902..4173)
                     /gene="waaA"
                     /locus_tag="WIB82_001657"
     CDS             complement(2902..4173)
                     /gene="waaA"
                     /locus_tag="WIB82_001657"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ3842930.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMTECRKSGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACWHALASGAAVQVESADAWREAVEKTLSSKGGGMQMQ
                     VRVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            complement(4236..5684)
                     /gene="gnd"
                     /locus_tag="WIB82_001658"
     CDS             complement(4236..5684)
                     /gene="gnd"
                     /locus_tag="WIB82_001658"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ3842931.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDERAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPTWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
BASE COUNT         1534 a         1828 c         1544 g         1483 t
ORIGIN      
        1 ctcggttgta atgttcgccc ccattcgatt caactcgggg acgtgcataa agcggttttc
       61 aaaaatcgtt tccaccacgc ggcagcttcc ctccgccacg gcattcaatg ccataaactg
      121 cgcctgcata tccgtgggga agccggggtg gacgaccgtg cggatgtcca ccgccttcgg
      181 acgctgccgc atatcgatgg cgatccaatc gtcgcccgct tcaatcaccg cacctgcctc
      241 aaccagtttg tccaacacca cttccatcgt tttcggcgcg gcattccgca aaaccaccct
      301 gccgccggtt atcgccaccg cgcacaggaa cgtccccgcc tcaatccggt cggggacgac
      361 gctgtgttcg cagccgtgca gctcgtccgc accttccaca atcattgtgg acgtaccgat
      421 gccgctgatt ttcgcgccca ttttgaccag gcattccgcc aaatcgacca cttcaggctc
      481 aatggcgcag ttttccaaaa ccgtcgtacc ttccgccagc gtcgccgcca tcagcaggtt
      541 ttccgtgccg ccgacggtaa cgacatccat cgccacgcgc gtacctttga gtttgccttt
      601 ggctttgacg taaccgtgtt cgataacaat ctcagcaccc atcgcttcca agcctttcaa
      661 atgctgatcg acggggcgcg aaccgatggc gcagccgccc ggcaggctga cttgcgcctc
      721 gccgaaacgc gccagcgtcg gccccagcac caaaatcgag gcccgcatcg tccgaaccaa
      781 ttcgtaaggg gcgcaggtgt tgtttaccgt accaccgttg atttcaaatt cactgatatt
      841 gtcggtcagg acgcgcgcgc ccatcccctg aagcagcttt tgtgtggttt tcacatctgc
      901 cagcataggc acatttttca ggcgcaacgt acccgatgtc agcaaacccg cgcacatcag
      961 cggcaatgcc gcgtttttcg cgcccgagac cgttatttcc ccgttgagcg ggccgtttgc
     1021 ggagattttc agtttgtcca cgtttgttct ttcctggtgg gtacttgtaa tatttcaata
     1081 ctcgggacaa cgcataaagc atcacccgat gaaggttgca gaggcggaat tataagggat
     1141 tttcgggaaa aatacggaag ccgcaccaaa gaatttgacg aaatgccgcg ctttccgaac
     1201 aaggattgtc ggaagacaaa aaaagccgag ttttgaaaac tcagcttttt ttgttttatc
     1261 tggtgggtcg tgagcgattc gaacgctcga ccaacggatt aaaagtccgc tgctctaccg
     1321 actgagctaa cgacccgata agctcgaaat tctatacatc aagcctgatg ctgtcaacca
     1381 ttttgtcggc gttcagacgg cattttattt atcaggctgt tttttcgtat aaattaacga
     1441 ggtcagtatc gatgcaccca acgcgccgaa cacgaccgac agcgaaacgg aaatcgggat
     1501 atgcacccaa tgcattacca gcattttcac accgataaaa ctcaacacga atgccaagcc
     1561 gtatttcagg aagataaagc gttcctccac atccgacagc aggaaataca tcgcccgcaa
     1621 acccaaaatg gcgaaaatat tggaggtcag cacgataaac ggatcggtgg taacggcaaa
     1681 gacggcgggg atgctgtcca cggcaaacac gacatcgctc aattcaatca tgaccagcac
     1741 caaaaacagc ggcgtggcga tttttttgcc gttttcgacg gtaaaaaatt tctcgccgtg
     1801 aaattccgtg ccgaccggaa cgactttctt gacggcattc agcagcctgc tgtttgccaa
     1861 atccccttcc tcatcgcctt cgggcttcat catgtgtatg ccggtataga gcaggaacgc
     1921 gccgaacaga tacagaatcc actcgaactg ccgaaccagt gccgcgccga cgaaaatcat
     1981 gacggtgcgc aataccaatg cgcccaatac gccgtacagc agcacgcggt gctgaaactg
     2041 cggtgcgact ttgaagtagc cgaatatcat caggaacacg aaaatattat cgactgccaa
     2101 cgatttttcc aaaatgtagc cggtaaagaa ttccaatact ttttcttttg cgactgccgc
     2161 gccgtagccg ggattgccgg cgagttcgaa atacagccag cccgcgaaca ggcaggatac
     2221 ggcaacccac aagccgctcc atgccaaggc ttctttgatg ccgactttat ggctgccgtt
     2281 tttcttcagc gaaaacatat ccaaggcaat catgaccagc actgccgcaa aaaaaacgcc
     2341 gtaaaacaac ggcgacccga tgccgggata ttctgtcatg gttcaatctc ctgatttgaa
     2401 atgtaattgt gttaccagct gatataaaac atcgcttttg ccaaaaagac aatcagcagc
     2461 atatgggtaa agacgacggt gtgtatgtat ttcgaccagc cgacggtcag tgtggaacgc
     2521 gccattttga cgacggcgat ggcgaagtgc gccaacacgc tgaacgccaa caggattttc
     2581 agcgtcagca tcgtaccgaa ggaagtggca aacggttcgc ccaatataga aagatagcgg
     2641 tttgccgcca tcacgatgcc gctggcgaac agcagtccga ccacaaacgg catcaccctg
     2701 acggcgcggt aagacattgc attttccact tcgcgccgcg cctcgcacga cacccgtccc
     2761 gtatgcagga cggacaaaac cagcacttca aaaaacacgc cgccgacaaa ggcaatagcg
     2821 caatacagat gaacgatgtg cgcgacggca taaatactca tacgatgctc caaacggaaa
     2881 actcggatac ggattgtatc actatcgccc ccgatgtccg cataccgctt cccgcaccgc
     2941 ctcggcgatt ctcgcgcccg ctccgcgatg ttgtgcgata aagccgtcca cgcgcacctg
     3001 catctgcatt ccgcccccct tggacgataa ggttttttca acggcttcac gccacgcatc
     3061 cgccgattcg acttgaaccg ccgcccccga tgccaaggcg tgccagcagg cttcggaaaa
     3121 attgtaggtt gaaaagccga atatcgtcgg aacgccgcag gaaagcggtt cgataatgtt
     3181 ctgacagccc gaatcgacca gactgccgcc gacaaaagcg acatcggcgc acaggtaata
     3241 cgcatacagc tcgcccatac tgtcgcctat ccacacctgc gtatcaggtt cgaccggcaa
     3301 accgtcgctg cgccgctgaa ccttaaaccc gaagcgtttt gccgtttcaa ataccgtctg
     3361 aaaatgctcg ggatggcgcg gcacgacgac cagcagcgca tcgccgcgat attgttgcca
     3421 cgccgccagc agtttttccg cctcgtcttc accccgataa acgcgcgtgc tgccgcacac
     3481 ggcaacctgc cggtctccga tgcgtttttc aaactgcccc gccagcgttt tcatctgttc
     3541 cgacggcatc aggtcgtatt tggtattgcc gcacacctgc acggatgccg cgcccaattt
     3601 cgccaaccgc gccgcatccg cctccgtctg cgccagacaa cccgtcagcg aagcggcggc
     3661 aggacggatc aggcggcgga ctttcagata accgtttagc gatttttccg acagccgcgc
     3721 attcgccaaa aacagcggaa cgcccgattt ccggcactcc gtcatcaggt tgggccagat
     3781 ttcggtttcc atcaaaatgc cgaacatcgg gcggtgttcg cgcaaaaact gccgtaccca
     3841 cgtttttttg tcatacggaa gataacggca ttgtgcatca ggaaacagaa cttgcgcggt
     3901 ttcccgtccc gtcggggtca tctgcgtcat cagcagcggc gcatcgggaa aacgctgccg
     3961 caactcgcgt atcaagggct gggcggcacg cgtttctccg accgaaacgg cgtgtatcca
     4021 aaccgcgccg gtaacgggat tcggatacgg cttgccgaaa cgctcgtccc gatgcgcccg
     4081 atatgccggg gcacttccgg agcgtttgtc caaataacgc cgtatccata tcggcgcaag
     4141 cagccacaat acatcataaa gccattggaa catctttcta tttcctgcaa aacaaatgcc
     4201 gtccgaacgg ttcggacggc atttcggcaa cggaatcaaa tatcgtaggt tgtcgaagcg
     4261 gtatctccgc ccttgcccgt ccagttggta tggaaaaact cgccgcgcgg tttgtcggtg
     4321 cgttcgtaag tgtgcgcgcc gaagtagtcg cgctgtgcct gcaagaggtt ggcaggcaga
     4381 cgttcggtcg tgtagccgtc caagaacgta atcgccgaag ccatgcaagg catagggatg
     4441 ccgcattcga ccgccttggc aaccaccttg cgccacgtcg gcaggcagtt ttccaaaata
     4501 tttttgaaat acggatccgc acccaagaac accaaatcgg gattgttttc atacgcgtcg
     4561 cggatattgc ttaagaatgc gctgcggatg atgcacccct cgcgccacag cagcgcagtg
     4621 ttgccgtagt ccaaatccca gccgtagctt tcgcccgctt cgcggatcag catgaagcct
     4681 tgtgcgtagg aaatgatttt agatgcaagc agggcctgtc tcaacgcctc aacccattct
     4741 tgtttgccgc cttcgacggg cgtaacggtt cgggcgaata gcttgccggt ctgcacgcgc
     4801 tgttctttga acgacgaaac gcagcgggcg aataccgctt cggaaatcag cgtcaacgga
     4861 atacccaaat ccaaagcatt gatgcccgtc catttgcccg tgcctttttg ccctgccgta
     4921 tcgaggattt tctcgaccag cggttcgccg ccttcgtcct tatagcccaa aattgccgct
     4981 gtgatttcaa tcagataaga atccagctcg gttttgttcc actcggcaaa cacgcggtgc
     5041 atttcgtcgt aagacagccc caagccgtct ttcatgaact ggtacgcttc gcaaatcaac
     5101 tgcatatcgc cgtattcgat gccgttatgc accattttga caaaatgccc cgcgccgtcc
     5161 ctgccgaccc agtcgcaaca cggttcgccc tgcggcgttt tggcggcaat cgcctgaaaa
     5221 atcggtttga cggcttccca agcgcgctca tctccgcccg gcatgataga cgggccgtgc
     5281 cgcgcgcctt cttcgccgcc ggatacgccc gcgccgacaa acaaaatccc tttttcggca
     5341 aggtaatgtg tccgccgtgt cgtgtcgggg taattggcat tgccgccgtc gataatgatg
     5401 tcgccttctt ccaacaacgg aagcagttgt tcgataaagt cgtcaaccac cgaacctgcg
     5461 cgaaccatca tcatgatttt tctcggtttt gccagcttat cgaccaagtc ttgcagggaa
     5521 tacgcgccga caatatccgt tcccttagcc gcgccgttta aaaattcgtc caccttaccg
     5581 attgttcggt tgtaggcgac aaccttaaat ccgcaatcgt tcatattcaa aatcaggttt
     5641 tgccccataa ccgccaaacc gattacacca atatcgcctt tcattgcagg aagctccgtt
     5701 atagatttaa tttatcgacc gcaactctac ctgatttaca cttgtttaac aatccttaac
     5761 tctttaattt tttgaaaaga tgcctttacg ctttaccgtg cgtttccctg aaggcttata
     5821 aataaaatat aaaaattaaa taaaaaaacg atgattatat tgataggaga aattttctgt
     5881 gggtaacttt tttttatttt aaaaatcatc aggattacct ttttttaggg tgtcggtaag
     5941 gcggattccc ttttgtgcat acctgtggat tgttttttat ggggaataag ttttgtggat
     6001 agtttgcttg ttgtgcagat ggcatcctac ttttctttac cgaatggctg ctggcgcctt
     6061 taagaacggg aatactgtgg agatttaagc cggaagcgtg tttggaactt gtggaaatgg
     6121 tcaggtgtcg gcacgaatgt cttatttctg catatcggca gagtgtgcat ccggatttgt
     6181 gtataagtgg tggaaaaaat gagattttcg ggtaaatctc acaatatttc agtcagataa
     6241 ctttggattg cttgtgtata agtaaacttt cggatgggga tacgtaacgg aaacctgtac
     6301 cgcgtcattc ccacgaacct acattccgtc attcccacga aagtgggaat gatgaaattt
     6361 tgagttttag gaatttaccg gaaaaaaca
//
LOCUS       DAWHRS010000120         6321 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWHRS010000120 DAWHRS010000000
VERSION     DAWHRS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.120"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(259..2187)
                     /gene="dnaK"
                     /locus_tag="WIB82_001659"
     CDS             complement(259..2187)
                     /gene="dnaK"
                     /locus_tag="WIB82_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ3842932.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2395..2667)
                     /locus_tag="WIB82_001660"
     CDS             complement(2395..2667)
                     /locus_tag="WIB82_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842933.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVALSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2853..3539
                     /locus_tag="WIB82_001661"
     CDS             2853..3539
                     /locus_tag="WIB82_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3842934.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3671..4009
                     /gene="erpA"
                     /locus_tag="WIB82_001662"
     CDS             3671..4009
                     /gene="erpA"
                     /locus_tag="WIB82_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ3842935.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            4026..4172
                     /locus_tag="WIB82_001663"
     CDS             4026..4172
                     /locus_tag="WIB82_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842936.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4169..4633
                     /locus_tag="WIB82_001664"
     CDS             4169..4633
                     /locus_tag="WIB82_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ3842937.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPTRNIKPLVFGLLIAFLGYLLGKIF"
     gene            4668..6179
                     /gene="ubiB"
                     /locus_tag="WIB82_001665"
     CDS             4668..6179
                     /gene="ubiB"
                     /locus_tag="WIB82_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ3842938.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1431 a         1395 c         1689 g         1806 t
ORIGIN      
        1 agatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt tctctttgag
       61 ctaaggcgag gcaacgctgt actggtttaa atttaatcca ctatataaaa aatttccaga
      121 gaaccgatac aacagttgga acttgggttt gggaatatta cggtagatga acttggaacc
      181 tctgttatgc tatggtcttt tatctcaatt gaaaaaagcg cgaatcaaac ggttcgcgct
      241 tttttcagac ggtattaatt attttttgtc gtcttttact tcttcaaagt cggcatctac
      301 gacatcatcg tctttctttg cagaagcatt ggcttgttcg ctttcgcctg cttgggcttc
      361 agcttgtgct tgagcgtaaa ccatttcccc cagtttttgg ctggctgtgc ccagcgcctc
      421 ggttttggca tcgatagcgg ctttgtcgtc gcctttaact gcttcttcgg cttctttcag
      481 cgcggcttcg attttttctt tctcggctgc gtcgagtttg tcgccgtagt cggccaaaga
      541 ttttttcaca gagtgaatca gggcttcagc ttggttgcgg gaagcgacca attcagtcag
      601 ttttttatct tcctcggcat tggcttcggc atctttcacc atgcgttcga tttcttcttc
      661 gctcaaacct gaagaacctt ggatggtgat gttggctgct ttgccagtgc ctttgtcttt
      721 ggcggaaacg tgcaggatac cgttggcgtc aatatcgaag gtgacttcga tttgcggcat
      781 accgcgcggt gcgggtgcga tgtcgcccaa gttgaactga cccaaagatt tgttggcaga
      841 agcgcgttcg cgttcgcctt gcagtacgtg gatggttact gcgctttggt tgtcttcggc
      901 ggtagagaac acttgcgacg ctttagtcgg gatagttgtg ttcttttgaa tcagcttggt
      961 catcacgccg cccatggttt cgatacccaa ggacagaggg gttacgtcca gcagcaatac
     1021 gtcgctgcgg ccgccgctta acacttcgcc ttgaatcgct gcgcctacgg cgacagcttc
     1081 gtcagggttc acgtctttgc gcggctcttt gccgaagaag tctttaacgg cttcttgtac
     1141 tttcggcata cggctttgac caccgaccaa aatcacgtcg tcgatgtcgc cagtgctcag
     1201 accggcatct ttcaatgcgg tgcggcaagg ctcgatagag cgggcaatca ggtcttcaac
     1261 caggctttcg aatttggcgc gggtaatttt catcgccaag tgtttcgggc cggttgcgtc
     1321 catggtgatg tacggcaggt tgatttcggt ttgctggccg ctggacaatt cgattttggc
     1381 tttttcggca gcttctttca gacgttgcag agccatgacg tcttgtttca aatcaatgcc
     1441 ttgttctttt ttaaactcgt caatgatgta gtcgatcaag cgttggtcga agtcttcacc
     1501 gcccaagaaa gtatcgccgt tggtagccaa tacttcaaat tgtttgtcgc cgtcgaggtt
     1561 ggcgatttcg atgatggaaa tatcgaaagt accgccgccc aagtcatata cggctacttt
     1621 gcggtctttg ttgtcgcctt tgtccatacc gaatgccaaa gcggctgcgg tcggctcgtt
     1681 gatgatgcgt tttacgtcca aaccggcgat acggcctgcg tctttggtgg cttgacgttg
     1741 gctgtcgttg aagtaggccg ggacggtaat cacggcttcg gttacttttt cgcccaagta
     1801 agcttcggcg gcttctttca ttttacgcag gacttctgcg gaaatttgag gaggagacag
     1861 ctctttgcct tgtgctttta cccatgcgtc gccgttgttg gctttgatga tttcgaaagg
     1921 catagattcg atgtcgcgtt ggacttcttt gtcttcaaat ttgtggccga tcaaacgttt
     1981 ggcggcgtaa atagtgtttt tggcgttggt caccgcttgg cgtttggcag gcgcgccgac
     2041 gaggatttcg ccgccgtcca aataagcgat aacggacggc gtggtgcgtg cgccttctgc
     2101 gttttcgata actttggttt gaccgttttc ggaaatggcc aaacaagagt tggttgtacc
     2161 taagtcgata ccgattactt ttgccatgtg gataatccta tttgattttg cttattttga
     2221 gaaatatgtt ggaacatttt gtcccgatgg gctgtaaata gggcgggcgg cgggctgttt
     2281 caagctacag catggctata agtatataac tttatgaata tattggtttt atatttgatt
     2341 taatacattt ggctccaatg cattcaagca taatgtttca aatggcaggc aggtttattc
     2401 atagacgatg ccggcgagca tttcctgttc gttcaagttg ccgtactctt tttcccagtc
     2461 gtgtgaagac tcgatgatgt cgcattcttt ggaaagggcg acttgttctg catccatatc
     2521 tttggcgttc agtatgttga attgttcgca cagggatgcg gataaagtga tgtcaggctg
     2581 tttggcttca gaacggtttt cttggaaggc aaagcagaat gcggtaaatg ccgcagcata
     2641 gataagatat ttgccggttt tcttcatttt ctatcctttt tctgtcaatt caggattaaa
     2701 cctatggaaa atcttaaaaa ttatgtattg agtaagaaaa atcataattt aaatttagtt
     2761 tatcatagtt gttccgtttt tgggatagct aaggtaaaat atatttcatg tttactttag
     2821 atgattgaat gaaggggagc ggaaggatat ttatggaaac ctttaaagac agactggttt
     2881 ttttatggaa aagcgaagcg aagcaggcaa aaatcgcatc cgatattgaa atgacgattg
     2941 cgggcttcag caggatatgg aatgaaggcg gtttgccgaa gtctgaaaca ttaaaaaaaa
     3001 tcaagcagtt gaaggggtgt agtatcgatt ggctgctgac cggggagggt aatccgtttc
     3061 cggatgaagc cccaaaaaaa tcccttgctt acgatacttt gggcaatgaa gtcgatacgg
     3121 acgagtttgt cttcgtgccg agatatgata ttcgggcggc tgcgggatac gggcagtttg
     3181 tcgatcatga ggaaccggta tttacaatgg cgttcagacg gcattggatt gagaattatg
     3241 ttacccgcga tacgaaaaac ctgtctgtaa tttccgtcaa gggggattcg atggaggggg
     3301 ttttgaatga cggcgattcg attttggtca atcatggtga aaatacgccg agggacggtc
     3361 tgtatgtgtt gcggattaat gaaaatctgc tggttaaacg tttacagatt gtaccgggcg
     3421 ggattatcaa tgtgatttct gcaaacgagg cttatcctgc ttttgaaatc aatttgaacg
     3481 atttgaccga tgatgtggag attatcgggc gtgtcgagtg gttcggcagg acgatttgag
     3541 tttggggctt gaaattgcag gcggtcaaac ttatctattg gaacaattcc tttttcaaag
     3601 gcgaagcctg cttgcctttg aagggggttt gagagagaat gcagaaaata ttatattaag
     3661 gaataacacc atgtcggatg aaagccctat tatttttact gacagctgct gtgccaaagt
     3721 tgccgatttg attgccgaag aaaacaatcc cgatttgaaa ttgcgggttt ttgtcaatgg
     3781 cggcggctgt tcgggtttcc agtacggatt tacttttgac gaaatcaaaa acgacgacga
     3841 ttttgaaatt gagaaaaacg gtttggtctt tttggtcgat ccgatgagct atcaatatct
     3901 ggtcggtgcg gaaatcgact atacggaaag tttgcagggt tcgcaattcg tcatccgcaa
     3961 tccgaatgcg gaaacaacct gcggttgcgg atcgtcgttt tccgtatgac cgcttggttt
     4021 gtgtgatgcc gtctgaacgt tcagacggca tttttacttt tagaaaatat attatcggga
     4081 tgaattcaca tataatccga ttgtttgaag atgaatcggg tttcccgaaa ggaacgggcg
     4141 gaacggtatc aggcgtattt gttcccttat gattgagatg agtaaagatt accgaaacga
     4201 tttgtacgat gtatatgttt cttacccgcc ccaagtggat cgcgggctta tccgggagtg
     4261 ccttaaggag aatctcggcg aggaaaaggc ggaaggattg atcgaatcgc tcgattccaa
     4321 acctcaagtg ctggttgagg aaaaatgcac ttgggcgaaa cgggaagagt tgcatgatta
     4381 tttcagctat ttgggtttgg atattattac ccggagatat atggagttgg aaacggtcgt
     4441 gccgccggag gaaggtgagg gcgaaggaga gggcgcggat ggggaaatgc ccgaatatct
     4501 tgaacttcat ggcgggcggg aagatgatat ttccgcacct tcgcaacccg aaccgccgac
     4561 ccgcaatatc aaaccgctgg ttttcgggct gctgattgcc tttttgggct atctgctcgg
     4621 taagattttt tgattgtccg ataaatgctg tattcgggat tttatatatg aaatggttga
     4681 aacgcctgac ggtcattgtc gggacttttt accgctatcg gctggcaggt ctgtgtgctt
     4741 cgctgatggg tagcggttgg atatgcgctc tgctgaaaat gatgccgcag tcgtccaaat
     4801 tgaaaaacga accgcctgct gtccgtctgc gccttgcctt ggaaagcctg gggccgattt
     4861 tcatcaagtt cgggcaggta ttatctacgc gccccgattt gattccgcac gattacgccg
     4921 tcgaactggc aaagctgcaa gacaaagtgc cgccttttga cgcgcggctt tcgcgtgaac
     4981 aaatcgaaaa atcgttgggt cagtccatcg aaaagctgta tgcggaattt gaaaccgagc
     5041 ccatcgccag cgcgtccatc gcccaagtac acaaagcccg cctgcattcg ggcgaacgag
     5101 tggcggttaa agttttgcgc cccaaccttt tgccccttat cgaacaggat ttgtcgctga
     5161 tgcgtttcgg cgcggcctgg gtcgaacgct tgttttcaga cggcaggcgt ctgaagccgc
     5221 tcgaagtggt ggcggagttc gacaaatacc tgcacgacga gttggatttg atgcgcgaag
     5281 ccgccaatgc cggacagctc gggcgcaatt tccaaaacag caatatgctg attgtgccca
     5341 aggtgtttta cgactactgc accagcgacg tgctgacaat cgaatggatg gacggcacgc
     5401 cggttgccga catcgccaaa ctcaaagcag acggcatcga tttgcacaaa ctcgccgatt
     5461 acggcgtgga aatctttttc acgcaggtct tccgcgacgg ctttttccac gcggatatgc
     5521 accccggcaa tattttggtt gccgccgaca accgctacat cgccctcgat ttcggcatcg
     5581 tcggcacgct gaccgattac gacaaacgtt atctcgccat caatttcctc gcctttttca
     5641 accgcgatta ccggcgcgtc gccaccgccc acatcgaatc gggctgggtg cctgccgaca
     5701 cgcgtgcgga agaattggaa gccgccgtcc gcgccgtgtg cgaaccggtg ttcaacaaac
     5761 cgatttcgca gatttccttc ggcttggtgc tgatgcgcct gtttgaagtc agccgccgct
     5821 tcaatgtcga aatccaaccg cagcttgtgc tgctgcaaaa aacgctgctc aacatcgaag
     5881 gcttgggccg ccagctcgat cccgatttgg acttgtggaa aaccgccaaa ccgtttttgg
     5941 tgaaatggat gaacgggcag gttggcccta aagccctttg gcgcaacctc aaaaacgaag
     6001 cccccgactg ggcgcaaatc attccttcat tgccgcgcaa aatcaatgcc ttggtcgatg
     6061 aggcgcgccg gcaggaaatg cgcgatgcct atattcattt ggttaaagtg cagcagcggc
     6121 aaagcctgtg gctggctgtg attgcggttg ttttgctgct gattttgctt ttgaaatagg
     6181 ctttgtccga attatcgccc gactccgccc gtttacaagg aaaccggtta tttccggttg
     6241 cgtgggaatc gggtgtatcg aataaaaggc attttgtccg actggcaagt gccgacatcg
     6301 gcggcatatc aaggcgcagg c
//
LOCUS       DAWHRS010000121         6246 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWHRS010000121 DAWHRS010000000
VERSION     DAWHRS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.121"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WIB82_001666"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WIB82_001666"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ3842939.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1297..2652)
                     /locus_tag="WIB82_001667"
     CDS             complement(1297..2652)
                     /locus_tag="WIB82_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ3842940.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAASKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(2667..2939)
                     /locus_tag="WIB82_001668"
     CDS             complement(2667..2939)
                     /locus_tag="WIB82_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ3842941.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3065..3802)
                     /locus_tag="WIB82_001669"
     CDS             complement(3065..3802)
                     /locus_tag="WIB82_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ3842942.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WIB82_001670"
     CDS             complement(4083..4982)
                     /gene="prmB"
                     /locus_tag="WIB82_001670"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ3842943.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5127..5720
                     /locus_tag="WIB82_001671"
     CDS             5127..5720
                     /locus_tag="WIB82_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842944.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHATSKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5832..>6246
                     /locus_tag="WIB82_001672"
     CDS             5832..>6246
                     /locus_tag="WIB82_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ3842945.1"
                     /translation="MTKLYAEIAKMETQDDGTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAV"
BASE COUNT         1680 a         1577 c         1455 g         1534 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgtttgg taaggaatga atttatgcgc
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcggat tttaaaggaa
      241 atgcacggag gcaagctgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcag agtacgaagc ggtcgaacaa tactcgctct ggccggcagt cggaaaccaa
      481 tggcaacttg aggctttgct ttcccgccat tggaaaaaac ctgtcaaggt ctggttgaaa
      541 atggattcgg gaatgcaccg taccggattt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaaac agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat gacggaaata cagatggaag cattcgattt gggtacggaa
      721 gggctggaag gcgaagaaag ccttgccaat tcggcggcta ttttgaatgt tcccgaagca
      781 cgcagggatt gggggcgcgc gggcttggcg ttatacggca tttccccgtt cggaggcagc
      841 gatgacaggc tgaaacccgt gatgaggctt tcaacccgta tttttggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgcgtc
      961 ggcctgattg catgcggtta tgcggacggt tatccgcgcc gcgccccaag caattccccc
     1021 gtcgccgtcg acggtaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg atgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca atactgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taatcgagtc caaacaaaaa tgccgtctga
     1261 agcctttcag acggcatttc cccatcaaaa ccgcaatcag tttttcatcg attggaccgg
     1321 agcgggaatt ctgccgcccc gtttgacgaa cacttcgcac gaaccttctt ttaccggcat
     1381 cacaggtgcg tagcccaaca ggccgccaaa ctcgacgctg tcgccgacgg ttttaccggt
     1441 taccggaata atgcgcacgg cagtggtttt actgttaatc ataccgatgg cggcttcgtc
     1501 ggcgataatg ccggaaatgg tgtgcgcggg tgtgtcgccg ggaacggcaa tcatatccaa
     1561 gccgaccgaa caaacggcgg tcatcgcttc gagtttatcc aacgtcagca cgcctgcttc
     1621 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccac tcaaaccgcc
     1681 aaccgcgctc gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     1741 tgctgttgtg ccgtgcgtac cgcagacgct caaacccatt tcttcaagaa tgcgcgccac
     1801 cgagtcgccg acggcagggg tcggtgccag cgacaagtcg agaataccaa acgggatatt
     1861 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtaattt tgaaagcagt
     1921 tttcttcact acttccgcaa cttcggtcaa tgtcgttgca tctgaatttt ccaacgcggc
     1981 ttttacgaca cctgggccgg atacgccgac attgataacg gcatccgctt cgcccgaacc
     2041 atgaaacgcg cccgccataa acgggttgtc ttccaccgca ttgcagaaca cgacgatttt
     2101 cgcacagccg aaaccttcgg gcgtaatttc agccgtgcgt ttgatggttt cgcctgccag
     2161 cttgaccgca tccatattga taccggcacg cgtactgccg acattgatgg agctgcacac
     2221 gatgtcggtg gtcttcatcg cttcgggaat ggaacggatt aacacctcgt ctgaaggcga
     2281 catgcctttt tgcaccagcg cggaaaagcc gccgataaag gacacgccga tggctttggc
     2341 agccttgtcc aaagtctgcg ccacgctgac gtaagaatcg gctttgctcg ccgccgcgat
     2401 ttgggcaatc ggcgtgacag aaatgcgctg attcacaatc ggcactccat atttggcaga
     2461 cagatatttt gccgtcgcca ccaagtcttt gccgaccgtg gtgattttgt tgtaaatgtt
     2521 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     2581 ggtgcggaca tcgaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     2641 ttggatactc atcacattcc tccggctcaa atgcggtgca tcgcttggaa gatttcttcg
     2701 ttttgcatac ggatgtcgag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaacc
     2761 tcctgccgcg atttgctgca ttttgaagtg tccaccaaga taatcatggt aaaaaaatca
     2821 tccatcagct gttggctgat attgaggatg ttgattcggt tttccgccaa aattttggaa
     2881 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     2941 ggcttactcc ttgcagatat ccgttaaagt ccgaaattat accaccgttg gattttgaag
     3001 aaatattgtc aacaatatat acatacaaaa tgccgtctga aactatttca gacagcatca
     3061 agattcaggg ttcgattaaa taaccatcct tatcccactg ggttttcctg accaacttgt
     3121 catcctgata aacagcctcg ctctttttag aaccatcttc ataccactcc aaaaccaccc
     3181 cgttgcgttg atggtggcgg atagacagtt ccgagagtaa tcggccgctt tcatcccaag
     3241 tcagaatttt ggcaggctca tcgttgacca taaccatttc cgtcttgata ctgccgtcgg
     3301 cataccattg cttccatacg ccgtttgcct tattttgctt aaactggatt tcgctttcct
     3361 tgccgccgtt acggtaatag cggtaccccg taccttcact caaaccattt ttataaggca
     3421 taacggcaga ttttttaccg ttcggatacc agttgaccca ctccccgtcc ggcttaccct
     3481 tgctgaagcc ccccgccatt tttttctgac cattaaaatg ccacaaaatc aacataccgt
     3541 tttgcagggt aggcacaaaa gatttgattt gcgttgaagc aacgatataa ggttcggaat
     3601 atttcttcat cgacggataa taaaaatcct gcgcgtgcgc aataccctcc gccacactat
     3661 attgcctgat ataagcggca gaagacatcg tcgccgtcag tttcccgttc tgattaaaat
     3721 aaacagaata ggtctgcgcc ggcaaagcgg ccgaaaaacc caacaggaca gttgaaaata
     3781 caatccgaga taattttttc attgcaatag cgatataaaa acaaggctgt gttttagtaa
     3841 tcttttgatt tcaattattt gcaagggaaa agacaattat tttccggtta ggaataaacc
     3901 tattctattg aatatattga agccaagtac gcctatcaac actatattaa aacactgcca
     3961 aaaacaatta acttataaac aatatggtaa ggatttctct gccaagcatc aaacccgaga
     4021 caacgtatcg taaaaatgcc gtctgaaaac aaatcgtctt cagacggcat ttccccttca
     4081 actcactctt cacccaataa ctgctcgcgc gtcaagagga aaacaaaacc gtcgcccccg
     4141 ctggtttcca accaagtaaa aggcaactcc ggatacgctg cttccaatac atccctgtta
     4201 tgcccgattt ccaccagcaa tacacctttg ggattcagaa actttgccgc attcagaaga
     4261 atctgcctgg tggcatccaa cccgtccgcc ccgctgccca atgccaattc cggttcgtgc
     4321 aaatactctt caggcaataa ctcaaccgat tccgcatcca cataaggcgg attggaaaca
     4381 atcaaatcat aagtgccttc caatccttca aacaaatccg tatgaataag ccggatgcgt
     4441 tcttccaaac cataatcttc gacattaatc cctgccactt ccaaagcatc caagctcaca
     4501 tcaaccgcat caatttgggc atcaggataa tgatgcgcca tctgaatggc aaggcaaccg
     4561 cttccggtgc aaagatccaa agcattatgc accaactcat cgtattctat ccaaggacga
     4621 agtccgtcac ccaacaattc ataaataaaa gaacgaggta tgattacgcg ctcatccaca
     4681 tagaaatcaa actctccctg ccatgcctgg tgtgtcaaat aagcggctgg aatgtgttcg
     4741 acagcacgac gctcaataac cgtcagcact tcctcttttt cagcttccaa gagttttgca
     4801 tcaagatatg gggcaagcat atccaaaggc aaattcaaag tatgcagaat caaataagct
     4861 gcttcatcat gcgcattatc cgttccatga ccaaaaaaga gccctgcctc attaaaacgg
     4921 ctgactgcaa aacgtaaaac atcgcggata gtcgtcaatt cttgtgctgc ctgattaaac
     4981 ataatatgaa ccattctgcg tatagatact tttaattata acagaaacaa caagcaaacc
     5041 ttttcatatc gccaaataac cacccaatct acccatacaa ctacataaat gcccgcgcga
     5101 aaaccatcgc ccgaacggaa acgacaatgg ccgacggtat gggcaacctg attggctggg
     5161 aaaaaacggg gcttgttgcc ggtaagcagt ggataaccgc aaaagacgac aaggtgtccg
     5221 atgtctgcaa tgccaacggc gagatgggcg taatcgggct ttacgagcct ttctcacacg
     5281 gcgcattgac gatacccggt catccgaact gccgatgcga ggttgtttcc gtatcgggtg
     5341 gcgaattggg ggaatttgcc gaaaaaaagg agcttcgtaa agcggctatg cagtatgcgc
     5401 gggataactt tatcggcaaa agctatgtca atgaaaacag cgggcatgaa ctgaaggtaa
     5461 cttggcaagg tgtgaaacac gctacgtcaa aggcaaatca ggcggaatta tccatcatga
     5521 caaaacttga tgacttattg cgctacgcaa aatatgaggg ttcttattcg gataggaaag
     5581 gtcatcctaa tattattgca gcacataagt atcgtgccgt tgccaaggtt gggaatgagt
     5641 ctttaaatat cggtgtgatt gtaagggaat ttccagacga ccataaacat tacgaccatt
     5701 tcatcttgaa ggatgaataa agcccttttg cagtgtcgtt ctggagcgga tagcgttaag
     5761 gcaagtacac ttccagcctt gaaaaagggc tttaaattca gcatgccatt tatacaggca
     5821 ggagtaaacc catgacaaag ttatacgcag aaatcgccaa gatggagacg caggacgacg
     5881 gcacggtcaa ggtttggggt tacgcttcaa gcgaggagat cgattcggac ggcgaagtca
     5941 tcgcggcggc agctatgaag gcggcgattc ccgattatat gaagtttgac gcggggcgcg
     6001 agatgcacgg ctcaaacgct gcgggaacgg caattgaaat caacgttgaa gacgacggca
     6061 taaccttttt cggtgctcat atcatcgacc ccgttgtcgt gtcgaaagtc aaaacaggcg
     6121 tttacaaagg cttttccatc ggcggcagcg ttaccgcccg cgatgatttg aacaagtcgc
     6181 aaatcacggg cttgaagctg acggaaatca gccttgtcga gcgccctgcc aatcccgatg
     6241 cggtgt
//
LOCUS       DAWHRS010000122         6143 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWHRS010000122 DAWHRS010000000
VERSION     DAWHRS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.122"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..152
                     /locus_tag="WIB82_001673"
     CDS             <1..152
                     /locus_tag="WIB82_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842946.1"
                     /translation="DVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHL
                     EKVFG"
     gene            complement(360..1745)
                     /gene="pntB"
                     /locus_tag="WIB82_001674"
     CDS             complement(360..1745)
                     /gene="pntB"
                     /locus_tag="WIB82_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ3842947.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFIGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSS
                     AASFAGQEVSEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1811..2194)
                     /locus_tag="WIB82_001675"
     CDS             complement(1811..2194)
                     /locus_tag="WIB82_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ3842948.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GAAARAHAAQQNGFEVFAPFAAAVLTAHATGNAAQSTINTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWVGGFVCTVGLFVVAA"
     gene            complement(2198..3739)
                     /locus_tag="WIB82_001676"
     CDS             complement(2198..3739)
                     /locus_tag="WIB82_001676"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ3842949.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKDVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            4029..4862
                     /gene="serB"
                     /locus_tag="WIB82_001677"
     CDS             4029..4862
                     /gene="serB"
                     /locus_tag="WIB82_001677"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ3842950.1"
                     /translation="MPHALVLQFPSAETLPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLSDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKVQAAADACINFGGLERVRGLF
                     G"
     gene            4926..>6143
                     /locus_tag="WIB82_001678"
     CDS             4926..>6143
                     /locus_tag="WIB82_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ3842951.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGTDGVS
                     ISFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGF"
BASE COUNT         1315 a         1603 c         1785 g         1440 t
ORIGIN      
        1 ttgatgtttt gtatatgcac ttccacaaag ccaacgagtt tttgggtatg acccgcctgc
       61 cgacattctt atgtaacgat gtggttaaaa atccgcaagt ggaaaaatac ttggcagatt
      121 atcaggcaca cttggaaaaa gtgttcggct aattaaaaat ccatcttcaa cacggagatg
      181 gattttgttt gtttcgttga ttttgtgtca gtttcagatg taggtgctta ttcggacggg
      241 ccgtctgaaa atgtttgccc caatgcaaaa aaatcactgc aaaccttcat aaacggggtt
      301 tgcagtgatt ttttcaaatc aaacagattg aaaacctgcg ccgaattgtt cagacggcat
      361 tattttttca gttcggacag aatgtcatcc acggttttct tcgcatctcc gaaacacatt
      421 acgctgtttt cgttgaagaa cagcgggttt tgtacacctg cgtagccggt attcatcgag
      481 cgtttgaaga cgacgacttc ttttgccttc catacttcca acacgggcat acccgcaatc
      541 gggctgttcg ggtcggtttg ggcggcagga ttgacggtat cgttcgcacc gatgaccaag
      601 accacatcgg tttcggggaa atcgtcgttg atttcgtcca tttccaacac gatgtcgtag
      661 gggactttgg cttcggcaag cagtacgttc atatgaccgg gcaggcggcc ggcgacgggg
      721 tggatgccga agcgtacttc tgtgccgttt ttacgcaaaa gctcggtgat ttcggcaacg
      781 gggtattgcg cctgtgcgac tgccatgccg tagcccgggg tgatgatgac gctgtttgcg
      841 cctttcagca tttcggcaac atcggcggct ttgacttcgc ggtattcgct gacttcttga
      901 ccggcaaaag atgccgcgct gctgtcgctg ccgaaaccac cggcaattac cgagacaaac
      961 gagcggttca tggctttgca cataatgtag gacagaattg cgccgcttga gccgaccagt
     1021 gcgccggtaa cgatgagcag gtcgttggag agcataaagc ctgccgctgc ggccgcccaa
     1081 ccggagtagg agttgagcat agatacgacc acgggcatat ctgcgccgcc gatggaggca
     1141 accaagtgcc agccgaatgc gagggcaatc agggtcataa tcagcaggat gaagccgctg
     1201 ccgtcaatgc cgataaatac gagcagcaac acaaacgata cggcgagcgc cagtgcgttg
     1261 agcttgtgtt tggcgggcag ttgcagcggg ctgctgctga ttttgccgtt gagtttgccg
     1321 aatgcgacca gcgagccggt aaaggttacc gcgccgatga aaatgcccaa atacacttcg
     1381 accagatgga tggtgtgcat atcgtgcgaa acgttgcccg gctcgatgta gctgttgaag
     1441 ccgaccaaaa ccgccgccaa gccgacgaag ctgtgcagca gggcaatcag ttcgggcatt
     1501 tcggtcattt ccaccttttt ggctttgtag atgccgattg ccgcgccgat gagcatggcg
     1561 atgatgatcc agcccagtcc gtgggtattg tcggaaaaaa cagttacaaa aagggcgacc
     1621 gccataccgg cgataccgga atagcagccc tgtttggcgg tttcctgttt ggacagcccc
     1681 gccagtgaga agatgaataa aattgcggca acgatatacg ccgctgttac gagtcctgaa
     1741 gacatggaaa ttctccgatt ttcgatgatt tgttttcaat gccgtccgaa ctgttcagac
     1801 ggtatctgtt tcaagcagcc acgacaaaca gcccgacggt gcagacaaat ccgcccaccc
     1861 acatcagcga gcgcaatgcg gctttgtcgg cgatatagca ccagataaag gcgaggcgga
     1921 acaggatgaa caggcccgca agcgtgttga tggtcgattg cgccgcattg ccggttgcgt
     1981 gtgccgtcaa aacggcggcg gcaaacggtg caaagacttc aaaaccgttt tgctgcgcgg
     2041 cgtgggcacg ggcggctgcg ccttgcgtat gcgccagaaa atcgcgcgga ttgtggttgt
     2101 ctttaaaccg gaatccgccc gcttttttgg catacgccgc acaaaaaagc ggcaataggc
     2161 aggcaatcag aatacaccaa taggcgaaag tcatggctta ccctttctta aacatattca
     2221 gcatacgccg tgttaccgca aagccgccga agatattgat gctggcaatc aggatggcga
     2281 tgaaggcaag cagggtaacg aaaccgttgc cttggctgat ttgcagcagc gcgccgacga
     2341 cgatgatgcc ggagatggcg ttggttaccg acatcagcgg tgtgtgcagc gagtggctga
     2401 cgttccaaac gacatagtag ccgatgacgc aggcgaggac gaagacgata aagtggttca
     2461 ggaatgctgc gggtgcgacc gcgccgaccc acagtaccaa cacggcggcg atgacggcgg
     2521 gcgcgagttt tttccacagg ggaacgggtt tcggttcggg cttggcggca ggcgcggctt
     2581 tttcagacgg cgtttgctgc ggttgggcgg aaacttgaat cggcggaggc gggaaggtga
     2641 tttcgccgtc gcgggtaacg gtcatgttgc ggataatcac gtcttcaaaa tccagcgtga
     2701 tttcgccgtc tttgttcggg cttaacaact tggtcaggtt gaccaggttg gtggcgtaaa
     2761 gctgggaaga ctgtccggca aggcggtttg ccatgtcggt gtagccgatg attttcacgc
     2821 cgttgccggt tacggacaat tcgcccggtc gggtgagttc gcagttgccg cccgtcgccg
     2881 ccgccaaatc gacgatgacg gagccggatt tcatgctttc caccatttct ttggtaatca
     2941 gcttgggcgc gggtttgccc ggaatggcgg cggtggtgat gatgatgtcc acgtctttcg
     3001 cctgctcggc aaagagcttc atctcggcgg cgataaattc gtcgctcatc actttggcat
     3061 agccgtcgcc gctgccgccc gattcttgcg ggaagtcgag tttcaaaaac ttgccgccca
     3121 tcgattcgat ttgttccgcc acttccaagc gggtatcgaa cgcgcgcacc actgcgccga
     3181 gcgagtttgc cgtaccgatc gccgccaaac ctgccacacc tgcgccaatc accaaaacct
     3241 gcgcgggcgg cactttgccg gcggcggtaa tttgaccggt gaagaaacgg ccgaaggcgt
     3301 tggcggcttc aattacggcg cggtagccgc tgatgtttgc catcgaagac aaagcgtcca
     3361 aagcctgcgc gcgcgaaatg cggggcacca tatccatcgc cagcgcgttc actttcttgg
     3421 cgcgcaaggc ttcgaccaaa gcctcgtttt ggcgcggcca caggaagctg acgatggttt
     3481 gaccttcgtt caaaagcggc agttcctgtt cggacggcgc gttgacctta taaatcaaag
     3541 ggcagaccca aaccgccgct ttgtcggcaa cggttgcgcc tgctgtttgg taagcggcat
     3601 cgtccaaact tgccgccaaa cctgcaccgc tttcgacaac ggtttcaaag cccagtttgc
     3661 ccagcagggc gacggtggcg ggcgtgcagg cgacgcgggt ttcgccggat aatgactcgc
     3721 gtgggatacc gattttcatc tctgaatcct ttttcgggta gtttatatgt atcgtgggtt
     3781 aaatttaaat cggggcaggg cggagcaacg ccgtaccggt ttaaaaccga ttcacttcaa
     3841 atgttaatat attttagata atccccttat aacgaatttt catcaggctg gcaatagttg
     3901 cggcattttc ccgtgttgtc cgacacatac tgccgtttgc gtttgaagtc attttccgat
     3961 tgcagtcccg cccgccgtaa cattctttgc cgtttcacta tataatccgc attttttgag
     4021 ccgccgttat gccgcacgcc ctcgtcctcc aatttccctc cgccgaaacc ctgccttccg
     4081 acttcccctc acgcctgccc gaacctgatt atgctgatga aaagcgtatg cgttttatcg
     4141 ttgaagaagg gttttcttta agcgaaaaag acgcggcgtt acttggcagc cgtcaaatcg
     4201 accacgccgt gttgtcggat atggattttg gcgaactcgg tttgattgtc agcgatatgg
     4261 attcgacgct gattaccatc gaatgcgtcg atgaaattgc ggcaggcgtg ggtttaaaag
     4321 acaaggtagc ggaaattacc gagcgttcga tgcgcggaga gttggatttc gagcagtcgt
     4381 tgcgcagccg cgttgcgctg ttggcgggat tggacgaaca gattttggcg gacgtttatg
     4441 aaaacgtttt gaagctctcg cccggtgcgg aattcttgtt ggacgaatgc aaaaggcacg
     4501 atgtgaaatt cctgctggtg tccggcgggt tcacattttt taccgaaagg ctgcaacaac
     4561 gactcggctt cgaataccaa cacgccaatg ttttggaaat tgaaaacggc aggttgaccg
     4621 gtcgtctgaa aggcagaatc atcgacgcgc aggcaaaggc ggatttattg cgcgaatacc
     4681 gcagccgcct cggattgcag ccgcatcagg tgttggcgat gggcgacggt gcgaacgata
     4741 ttccgatgct caaagaagcg ggcataggcg tggcttaccg tgccaaaccg aaagtgcagg
     4801 cagccgccga tgcctgtatc aacttcggcg gtttggagcg tgtacgcggc ctgttcggat
     4861 aggcggatag gaaacggatg ccgtctgaaa ggttttcaga cggcatttga acggcaggaa
     4921 cgacagtggg acgcagaaaa ctttggtttg ccctggcagc agcgggcgtt atcggcggtt
     4981 tggtcggcat tgtgctgacg gaactgatgc acttcataca acatacggca tacggttatg
     5041 gcacggacgg cgtgtccatc tcgttccgcg aaggtgtggc acaggcttcc ggtatgcggc
     5101 gcgttgccgt gctgacgctg tgcggcgcgg tcgcaggcag cggctggtgg ttgctgaaac
     5161 gtttcggcaa gccgcaaatc gaaatcaaag ccgccttgaa acagccgttg caggggctgc
     5221 cgtttctgac gacggttttc catgttctgc tgcaaatcat aacggtcgga ctcggttcac
     5281 cgctcggacg ggaagtcgcc ccgcgtgaaa tgactgccgc gttcgctttt gccggcggca
     5341 ggcggttggg tttggatgaa gacgaaatgc ggctgctgat tgcttgcgct tcgggtgcgg
     5401 gtttggcggc cgtgtataac gtgccgctcg cctccacact ttttatcctc gaagccatgc
     5461 tgggcgtgtg gacgcagcaa gccgtcgccg ccgcattgct gacttccgtc atcgccaccg
     5521 ccgtcgcgcg catcggctta ggcgacgtgc agcaatatca tcccgccaac cttgccgtaa
     5581 acacttcatt actttggttt tccgccgtca tcggcccgat actgggcgca accgccgtct
     5641 ttttccagcg caccgcccaa aaattcccct tcatcaagcg cgacaacatc aaaatcattc
     5701 ccttggcagt ctgcatgttt gcgctcatcg gcgtgattgc cgtttggttt ccagaaattt
     5761 tgggcaacgg caaagcaggc aaccaactga cctttggcgg tttgaccggt tggcaacaca
     5821 gccttgagct gaccgccgtc aaatggctgg ttgtcttgat ggcgttggcg gcaggcgcat
     5881 acggcggact gattacgccg tctatgatgc tcggcagcac catttctttt gcagccgccg
     5941 ccgcgtggaa cagcgttttc cccgaaatgc cgtctggaag cgcggcagtc gtcggagcgg
     6001 cggtgtttct cggcgtatcg ctcaaaatgc cgctgaccgc cattgtgttc gtattggaac
     6061 tgacctacgc gccgctgtcc ctgctgctgc ctttgtgcat cggcatggcg ggggcattgg
     6121 gaacatcggg aaaaatgggc ttc
//
LOCUS       DAWHRS010000123         6141 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWHRS010000123 DAWHRS010000000
VERSION     DAWHRS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.123"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(275..709)
                     /locus_tag="WIB82_001679"
     CDS             complement(275..709)
                     /locus_tag="WIB82_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ3842952.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(712..1374)
                     /gene="exbB"
                     /locus_tag="WIB82_001680"
     CDS             complement(712..1374)
                     /gene="exbB"
                     /locus_tag="WIB82_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ3842953.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1440..2282)
                     /locus_tag="WIB82_001681"
     CDS             complement(1440..2282)
                     /locus_tag="WIB82_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ3842954.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2537..3175
                     /locus_tag="WIB82_001682"
     CDS             2537..3175
                     /locus_tag="WIB82_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ3842955.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3626..4426)
                     /locus_tag="WIB82_001683"
     CDS             complement(3626..4426)
                     /locus_tag="WIB82_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ3842956.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMVVFVIVLFGARNILKLAK"
     gene            complement(4562..5065)
                     /locus_tag="WIB82_001684"
     CDS             complement(4562..5065)
                     /locus_tag="WIB82_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842957.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5402..6043)
                     /locus_tag="WIB82_001685"
     CDS             complement(5402..6043)
                     /locus_tag="WIB82_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ3842958.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
BASE COUNT         1631 a         1544 c         1278 g         1688 t
ORIGIN      
        1 cggcttcgtt gccttgtcct gatttttgtt aatccactat aaaaattaga aatgcacatt
       61 ttcattattc tcgcgcaggc aggactccag acttacccat ttcagtaatg tttgaaaata
      121 aaagaaaaat cagatgtttg tattcccgcc tgcgcagaaa tggagacggt gctctgtcgt
      181 ctcatttttg ttttaatcaa ctatatatag ctgattaaac ataagaaatg ccgtctgaaa
      241 gactttcaga cggcattcgt tcaagcgtcg aactttattg cgccttggtt tcggttacaa
      301 aaccgatttt ggtgattcct gcctgacggg cggcttctaa agctttgttt acataatcgt
      361 attccaccgc cttgtctgcc gcaatcgcca caatcacgtt ttcattctgc tccttggcgg
      421 ctttcagacg gctttccact tccccgattt ccactttgct tgcagaatcc ccgccgacat
      481 aatagccgcc gttcgcatca atcgtcaggc gcagggggtc tttaggctgt ttgtcctgct
      541 tgtttgtctg ctcggacgcg gtcggcagtt ccaaagggat ggaatgcgtc agcaccggca
      601 tagtaatcat aaacacaatc agcaacacca gcatcacgtc caccaacggc gtaacgttga
      661 tgtcggacat cggagaatcg tcgccggaat tcatcgaacc aaatgccata atcagctatc
      721 cttttgatta agcaggcgga cgtgcaaatc gtgcgccatc gcatccaaat cctgggtcag
      781 tatttttgtg ccgcgattga ggaagttgta tgccaacacc gccggaatcg ccacgaacaa
      841 acccgccgcc gtcgccacca gtgcctcgcc aatcgggccg gcaaccgccg caatactcat
      901 ctgcccgctt tgcccgatat tgatcagggc gtggtaaatc ccccaaaccg tgccgaacag
      961 cccgataaac ggcgcggtcg cgccgatgga ggcaagcgcg gtcatcccgt aatcaaaccg
     1021 gcgcataatc tgcgccatac tgttgcggat ttgaatgacc aaatactcgt tcaacggcaa
     1081 agcctgcgcc agttcggacg cttcgtttcg gcggtagttg cggtaagact gcaatgcctc
     1141 ttgcgccagt ttggacaaag gcgcatcgac ggcgcgcact ttttcgaccg cgtcgttcag
     1201 cgacaaagta tcgcgcatat gccgtttgac ggcggcattc cctttgcgcg cccgatacag
     1261 cttgatgcag cgcaagacaa ccaaacacca cgttacgata ctcatcaaca gcatcaacac
     1321 aaacacacca atcaggacgg gatcgcccga ttcaaacact aatttcaaat tcataatgat
     1381 tccaacactg aaaaaaccaa tcaaacatcc aagctgccgc aaaccgctgc ggcaaccgcc
     1441 taattcaatt caaacttgac ggggacttta aactccgtcc aggcattggc ttgaaaatgc
     1501 ccgttttgcg ccgccttgcg tgccgcattg tccaaccggg aaaaaccact gcttttcacg
     1561 attttaacgg actcaacatg accgcccgga gaaaccaaaa cgctcaaaac aaccgtaccc
     1621 tgctcgtcat tctccataga aagcgtggga taagccgggc gcggaatgct gccgttggcg
     1681 cgtaaaggat tgcctttgct gctgccggct ccttccccgt gttcgccttt gacaccgccg
     1741 ctacctttac cgctgccttc tccgcgcccc gttccgtctc ctttggtacc agttccctta
     1801 tcttccccat tgccctgctc gctgtctgct ttggcagaag cattgccggg atgttcggca
     1861 ggtttttcag acggcttctc gaccggtttt tccgccggtt tcgggacagg cttcgcttcc
     1921 ggcttaggct ctggtttcgg tttttcttcg ggtttcggct tttcttcagg tttcggctct
     1981 tccttaggct gctgaatatc cgcatccgcc tttttcgtaa ccaccggctt caaaaccggc
     2041 ttgggcggct cgacaggttt gggcggctcg ggcacgggtt gcggttcggg cgcagcaggc
     2101 gcgcctgcac cttcgggggc gccgtcccct ccgccaaaat cgcccaaatc gacaaattca
     2161 ataacattgc ctgactctat cacgggcagc ttgtgcgcct gccagagcaa tgccaccatt
     2221 gccaaatgca gcagtgcgac ggaaaacacg actgcggggg ttaaaattcg ttctttatcc
     2281 ataattcggg cataataata gcaacaattc ctatttgcaa cctattttta caatttttgg
     2341 tcatatgaat gtctgttccg ttcacaggca aaccgtgttt aaacgctgta ttacagcaaa
     2401 tcatcagata acgggccggc agaaaaaatg attccgtctg atttcttatt ccaataaaat
     2461 caggttagat gatatattgc cgcttctgtc tgtcagccgt ttcgggctgc acaccacatc
     2521 tgttcaaagg aaaaccatgt ttcaaaattt tgatttgggc gtgtttctgc ttgccgtcct
     2581 gcccgtgctg ctcgccatta ccgtcaggga ggtggcgcgc ggctatacgg cgcgctactg
     2641 gggagacaac actgccgaac aatacggcag gctgacactg aaccccctgc cccatatcga
     2701 tttggtcggc acaatcatcg taccgctgct tactttgatg ttcacgccct tcctgttcgg
     2761 ctgggcgcgt ccgattccta tcgattcgcg caacttccgc aacccgcgcc ttgcctggcg
     2821 ttgcgttgcc gcgtccggcc cgctgtcgaa tctagcgatg gctgttctgt ggggcgtggt
     2881 tttggtgctg actccgtatg tcggcggggc gtatcagatg ccgttggctc aaatggcaaa
     2941 ctacggtatt ctgatcaatg cgattctgtt cgcgctcaac atcatcccca tcctgccttg
     3001 ggacggcggc attttcatcg acaccttcct gtcggcgaaa tattcgcaag cgttccgcaa
     3061 aatcgaacct tatgggacgt ggattatcct actgctgatg ctgaccgggg ttttgggtgc
     3121 gtttattgca ccgattgtgc ggctggtgat tgcgtttgtg cagatgttcg tctgactggc
     3181 tttcagacgg cataaacgct ccagaaaacg cggcaggaca tattgccctg ccgcgttttc
     3241 ctgtagtgta atcttatttt ttcatcatta ttagaaccag gttgcatgat aatacctttc
     3301 attaactgaa acactgatta agaaactcca gtctgtctaa tgatgaggtt ttcacatcgc
     3361 caaaacttgc caatcaaatg ctggatttat tgccgtctga gatttggtca aatccaaagg
     3421 cgacattctt agaccctgtg tgtaaatcag gggtattttt gcgtgaaatc gtcaaacgct
     3481 tggatgaagg cttgaccaat caaataccag ataaacaaac tcgcattaac cacattttaa
     3541 aaaatcaagt ttttggaagt actgccacgt atgtaggtag ctttgaccga tatttgcata
     3601 aaaactcctt tgctggtgaa aggaattatt ttgccaattt taaaatattt ctggcaccaa
     3661 atagtacaat gacaaagaca accatgccaa tgattaaatc aggatagcta gaatgagtca
     3721 ataacgtcaa tgctcccgcc gctatcacac cgatattgat gataatgtca ttggatgtaa
     3781 aaatcatgct ggctttgata tggatttctt tattttgatt tttgctcagt agatataagc
     3841 acagccagtt tgcaatcaat gccaaaaatg ccgtgccaat catcagttga taattgggca
     3901 gctgctcagc accgataaaa cgcctaatca cttctatcac cccaaataac gccaatatta
     3961 tctgcgttat ccccgccaaa aatgccacac gttttttata cgccagcgtc ataccaatgg
     4021 ctgatagcgc caatatatag acaaagctgt ccgccagcat atctagacta tcagcaatca
     4081 gccccataga attagcaaaa ataccaaccg aacactctat gataaaaaac acaaagttaa
     4141 tcatgagcac ttgatataat aatctttttt ctaagtgctc atcaggcttg ttaaacacta
     4201 tcttatcaac aatcacttcg gtggaaatga tatgactatc aaaattaagc ggttcaagta
     4261 cttgtaaaat cgttgtatct tgattatcgt gatagacggt taagcaccgc ccagcaatat
     4321 caaactgtaa ttcataaata tcagacacat cttttaaacg catgcgaatg agctgttctt
     4381 cggacgggca gtccattttg gtaatgttaa aaatggtctt tttcatctat ttagttcctt
     4441 gttttgatca ggttggctca aataaatctg tgtttatatt gctgcttggt aatttttgga
     4501 tggtttgagt aaattgatta ggttaaaatt tacctttgga agtaccgcca cgcataatag
     4561 tttagatatg tttataatct ctggataaaa aaacgtaata agtgcttact ggataacaaa
     4621 gtccaaacca atagcaggca aaataaggca tccacccccc ttcttcatta aggatatata
     4681 ttgagaaaca aatcgcaact aaacagaaaa aacttgggag ataaagccat ttcattcccc
     4741 tattcaagaa tctagccaag ataggtattt tgtattctac aaaaaagaaa ggcatttcca
     4801 agggaaacat gtcagataaa aacttttgtt tattttttac tatagataga accttgcttc
     4861 tcaagagaaa gccattaata ataccgatga cagctattaa tatatagaga atagtataag
     4921 tatgaataat cttcattaga caaaaagaag aaatggcaga taaattacat acgatatatt
     4981 ggaatataaa atatttacgg tctaaacctt gttcagttgc aattttttta aaattgcctt
     5041 gcataaaaaa atcaaaggcg tccattaaac tatctttcac attagaaatt taaaagctaa
     5101 ataatacgac aaacaatgtg aagtactatt catggtttat ttaaaaaata atactattct
     5161 gaacattatt tagatacaga aattaacaaa ttagaactaa acaagctttt aaatacttta
     5221 attttattgg aaagctataa aaggaactat aactttacac actagtcact tctttttaag
     5281 aggcaaaagg gattgggaag gtcgtcttgg agataagcac tggtatttcg gccaatggta
     5341 aatagagttt acctcaaata gggtagaacc tccttcatct gtcagttaat aacagccact
     5401 tttacaatgc cctgtcaaaa taaagcggca cgcccgattt ttcactcatc gtcatcaaat
     5461 aacccatcac cttttggggc cattcgatgc cgcgcaccac ggtcagattc ctcaaaacgg
     5521 ggaaaaccaa aatatcctcc ataccgattc cgccgttgat gccgtctgaa gcaccgtcca
     5581 tcaaattttc caactcttgc aaatctgcgt ttatccgttc gaggtattgg gcggttttat
     5641 tcaaattggc ggaaaagctg ccgatgcttt tctctttttt gtctgtaaaa tatttcaccg
     5701 cttccggcgt tgcaaattca ggcagcccga ttttgatcac gcgcggctgc accagtttgt
     5761 cgttgtatcc gcccaccttg tccagccacg cccgtatctc ggggcggact tcgtctttca
     5821 gacggtcttc gcggtcgaaa tgccgcacaa tgtccaaact ctcgcccata aacgaaccgt
     5881 cttctttttg caggacgggc acttgtttcg caccgatcat accgatcggc gttgcctcgt
     5941 cgtcgtttgc cagcacggct tcttcaacgt ccgcgccaaa cagcccggca gccatccgcg
     6001 cacgcacgca aaacgggcaa tggtcgtaaa tatacagttt catcaaaata ttcctcgtca
     6061 acctgtcggt accggctacc ttaacacccg gcgccgcccg aaacaagttt atcttcccgc
     6121 ctatgcaccg taaataaata a
//
LOCUS       DAWHRS010000124         6136 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWHRS010000124 DAWHRS010000000
VERSION     DAWHRS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.124"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            141..1538
                     /gene="aspA"
                     /locus_tag="WIB82_001686"
     CDS             141..1538
                     /gene="aspA"
                     /locus_tag="WIB82_001686"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ3842959.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1610..2485)
                     /locus_tag="WIB82_001687"
     CDS             complement(1610..2485)
                     /locus_tag="WIB82_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3842960.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2487..3209)
                     /locus_tag="WIB82_001688"
     CDS             complement(2487..3209)
                     /locus_tag="WIB82_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ3842961.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3232..3711)
                     /gene="ybaK"
                     /locus_tag="WIB82_001689"
     CDS             complement(3232..3711)
                     /gene="ybaK"
                     /locus_tag="WIB82_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ3842962.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3786..4148
                     /locus_tag="WIB82_001690"
     CDS             3786..4148
                     /locus_tag="WIB82_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ3842963.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            4183..5046
                     /locus_tag="WIB82_001691"
     CDS             4183..5046
                     /locus_tag="WIB82_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ3842964.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINHLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPSLAFTAECGSDGICSVVLPHRGILHIAPKNALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKTLTVPNGGIFKA
                     VHLFFKTENAE"
     gene            5139..6098
                     /gene="ttcA"
                     /locus_tag="WIB82_001692"
     CDS             5139..6098
                     /gene="ttcA"
                     /locus_tag="WIB82_001692"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ3842965.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGS"
BASE COUNT         1559 a         1782 c         1484 g         1311 t
ORIGIN      
        1 ccgaaaacaa cgtttcccgt tttcagacgg catacgatat ggacaatttc atccggccgg
       61 tatagtgttc gtcctgcggc ggcatctttc cgatggcgca ttttttcaat attaattatc
      121 cgacttacaa aggtaaaaac atgactgtcc gtatcgaaca cgatttattg ggcgaccgcg
      181 agattcccgc cgaagtgtat tggggcatcc acaccctgcg cgccattgaa aactttaaaa
      241 tctccacaca aaaaatttct gacgtgccgc agtttgtccg cagtatggtg atggtgaaaa
      301 aggcgacggc acaggcaaac ggtaagttgg gcgcggtcaa gcccgaaatc gccgccgcca
      361 ttgaaaaggc ttgcgacgaa gttctgctga acaaccgctg cctcgaccaa ttcccgtccg
      421 atgtgtatca gggcggtgcg ggtacttcgg tcaatatgaa caccaacgaa gtcatcgcca
      481 acctcgcatt ggaagtcttg ggatacgaaa aaggccgcta cgatatcgtc aatccgatgg
      541 atcatgtcaa cgccagccaa tccaccaacg atgcctaccc taccggcttc cgccttgccg
      601 tgtattacag catcggcgaa ttgctcgaca aactggccgt cctgaaaaac gcctttgccg
      661 ccaaagccga agagtttaaa gacgttttga aaatgggccg cacccagctt caagatgccg
      721 tgccaatgac tacaggacaa gaattccaat ctttccaagt gttgttggaa gaagaaatcc
      781 tcaacctcga ccgcacgcgc caactgctgt tggaagtcaa tttgggcgca acggcaatcg
      841 gcacgggcgt gaacacgccc aaaggctacg ccgaactggt ggtcaaaaaa ctctccgaag
      901 tcagcggctt gccttgcaaa ctgaccgaaa acctgattga ggcgacctcc gactgcggcg
      961 catatgtgat ggtacacggc gcattgaagc gcacggcggt caaactctcc aaaatctgca
     1021 acgacttgcg cctgctctct tccggcccgc gtgccggctt gaaagaaatc aacctgcccg
     1081 aattacaggc cggttcttcc attatgcctg ccaaagtcaa tcccgtgatt cccgaagtgg
     1141 tcaaccaagt ctgcttcaaa gtcatcggca acgatacgac gattaccttc gccgccgaag
     1201 ccgggcaact gcaattaaac gtgatggagc cggtcatcgc ccaatgtatg tttgaaacca
     1261 tttccctctt gggcaatgca gcaatcaacc tgtccgacaa atgcgtcaaa ggcattacgg
     1321 tcaaccgcga aatctgcgaa cgttacgttt tcaactccat cggtttggtc acttatctga
     1381 atccgtacat cggccaccac aacggcgact tggtcggcaa aatctgcgcc cagaccggca
     1441 aaggcgtgcg cgaggttgtc ttggaacgcg ggctgttgag cgaagaagaa ctcaaccgca
     1501 tcctctctcc tgaaaacctg atgaaccccc atctgtaacg ttgcagacaa atgccgtctg
     1561 aagcagggat gctgcttcag acggcatttt gttatatcaa tcagccggat tatatgccga
     1621 acagtacctt gacttccccc caataaaaca ggcaggcgat agaacacagt gccaacacga
     1681 ttcccgccgt attgattgtg ttgatttttt ccttaaagaa caatgcgccc gaaagcgtac
     1741 ccaaaacaat cacgccgata ttcataccgg caaaaaccaa ggtcggatta tccttcatca
     1801 tttggtgcgc ggtgatgtag gttacgatat tcataaaatt caaaccgccc aagaatatgc
     1861 cgccaaccac actctcaaca cgccatctga ccgatttggc aaacaggcag gcaaacatca
     1921 gcacacccgc cagcacaaat gcaaccagca ggttgcccgc aaatgccgtt ccgcttttgg
     1981 caagctgttt gaacaggata tcgataatgc cgtaacctgc ccacacgccc agcagcaatg
     2041 ccgcctgccg ccacgcgctt cctgattttt tgccaccgct gtgtttccaa agcaggcaga
     2101 acagtgcggc aaatgcgagg cacagcccga ttagtctgcc ttcgctgagt ttttcgccaa
     2161 acagcgtcaa ggcggcaaca atcggcaaaa acagcgacaa acgctgcgcc gcgtcggatt
     2221 tgacgatacc ggcggattct acggatttgc ccataatgac aaatacggac ggcagcagca
     2281 cgcccaatgc ggcaaacagc caccatgtcg gcaaaaatgc gccgatattg ccgacatccg
     2341 gcttcaatac cagcagggtc agtatgaccg cgaccacata attgacggcg accgcctgcg
     2401 cgatgtcgat ttttttcttc cttgccattt tcagcaaaat ggaaaccgac acgctgaaca
     2461 cgatgctgat taacaggtaa gccatactat atccatttct cgggtgcaac cgccctgccg
     2521 ccgttgaagc ggataaagcc ggcgatgatg cggtagaaat accagacgtt cgttgcaata
     2581 ataatcagca cgccgatacc caaaagggca gtcagcgcac ccaaaatata aagccaaaat
     2641 gtgcgccaaa aggttttaat caggtattcg gtatgtgcgg catagacaat gctgtccaaa
     2701 ctgttccgtt tcatataggc gagaatcacg ccgactatgg gcatataggc gaaaatcacg
     2761 ccgactatgg gcatgaacag catgattgca cccaaaccga acaggatgta ggcggcgagg
     2821 acatagttgc gcccgcttcc gccgccggac ggttcgtaag tatggcacga acgtgatgcc
     2881 tgccgttcaa acgcctgtct gaacgcctgt tcgcggcgca tctgttcgcg gtaaaactgc
     2941 tccctgcgcg cctgttcgcg gcggaatgcc tcttcctgcc gcccgcgttc ctcatgccgt
     3001 ctgaaggacg cgtcgtattg catccgtttc gacaggtcgg aaagcgtatc gtaagcccgt
     3061 tggatttctt tgaacttttc ttccgccttc ggattgcccg gattgcggtc gggatgatat
     3121 ttcatcgcca gcttgcggta ggcgcgtttg atttcgtccg ctcccgcctg cggcgacacg
     3181 cccaatacgg catacaaatc tttgtccata ttgttccttt atactttccc gtcaaaccgc
     3241 cgcctgtatt gttttcgggt tcaaaatatt taaatctccg ggacggatgc cgataatgaa
     3301 cccgcgtttt ccgccgttga tatagatggt ttccaaatcc atcaccgact gttcgacgta
     3361 aatatccaac tttgtccgga tgccgaacgg cgttgtgccg ccgaccagat agcccgtcca
     3421 cttgtttgcc tgtacgggcg tggcgggttc aatgtgtttc gcacccaaat gccgcgccag
     3481 attgcgggtt gaaatctgct tgtcgccgtg catcaagaca atcagcccct taccgttttc
     3541 atcttgcaaa acaatggttt taatgaccaa gtgttcgtct ttgccgaata gtcgggcaaa
     3601 ctgcgccgtg ccgccgtgtt cctcataggc ataggtaaaa ggttcaaatt cgatgccgtt
     3661 ttcacgcaaa acgcgcacgg caggtgttat cggataagtg tgtttactca tttttcgtca
     3721 tgcaaaacat ggaaatcggt caagcgcaat gttaccatat atccccttta tcgaaggaaa
     3781 ccgatatgga tatccgatat ttcggcacaa cgccccgcta ttccgaagcc gtcggcgcaa
     3841 acggcttgat tttcctctcc ggcatggttc ccgaaaacgg tgaaacggct gccgaacaga
     3901 ctgccgacgt gcttgcccaa atcgaccgct ggctggcgga atgcggctcg gacaaagcgc
     3961 acgttttgga tgcggtcatc tatctgcgcg atatgggcga ctatgcggaa atgaacggcg
     4021 tgtgggacgc ttgggttgcc gccggcagaa cgcccgcccg cgcctgcgtg gaagcacggc
     4081 tcgcccgtcc ggagtggcgc gtcgaaatca aaataaccgc cgtgaagaga gacgcggcaa
     4141 ctgcataaac agatttcctt tatatttcag agtacgcaaa tgatgaaaac cttactgccc
     4201 tttttcggcg tggtgcgcgt cagcggcgaa gaccgccaaa ccttcctgca cggacagctt
     4261 tccaacgaca tcaaccacct tcagaccggg caggcgtgtt atgcgactta caacacgccc
     4321 aaaggccgcg tcatcgccaa tatgattgtc gtcaaccgag gcggcgattt gctgctgatt
     4381 atggcgcaag acctgcttga agcaacggtc aaacgtttgc ggatgtttgt gttacgcgcc
     4441 aaagccgttt ttgaaatcct tgaagattac gccgtcggcg cggaattaga agcatccgcc
     4501 gaaccgcttg ccgcgcaaga acccagcctc gcctttaccg ccgaatgcgg ttcagacggc
     4561 atctgttccg tcgtcctgcc acatcggggc attttgcaca tcgcccccaa aaacgccttg
     4621 ccgccttatg atgccgccgc cgaaaacgcg tggcggctgc acgaaatccg tagcggttat
     4681 ccgtggatat gcgcggctac caaagaaacc gccgtcgccc aaatgctcaa ccagcacatc
     4741 atcggcggcg tacacttcaa aaaaggctgc tatccgggac aggaaatcat tgcccgtgcc
     4801 cagtatcggg ggcaggtcaa acgcggcttg gcggtattgt cgggaaattc ggccgtcgaa
     4861 gcaggaaccc tgctgacggc ggacggcgaa gaggccggca tcgtactcga cagcgttcaa
     4921 gattcggaaa actttaccgc ccttaccgtg attaaattct ccgccgcaca aaaaacactg
     4981 accgtcccaa acggcggtat cttcaaagcg gttcatctct tctttaaaac cgaaaatgca
     5041 gaataagccg ccgatgccgt ctgaagccct tcagacggca ttgccgcccc gccaatcggt
     5101 tacaatgccg cctttctaat tttcaaaccc aaaccgccat gtccaaaaaa accaaacaag
     5161 aactcgaaaa caacaaactc agcaaacgcc tgcgccacgc cgtcggcgac gcgattaacg
     5221 atttcaatat gatcgagccg gacgacaaaa tcatggtctg cctctccggc ggcaaagaca
     5281 gctatgccct gttagacatc ctgcgccagc tccaagccag cgcgccgatt gatttccaac
     5341 tggttgccgt caatctcgac caaaagcagc cgggcttccc cgaagaagta ttgccgacct
     5401 atctcgaaag catcggcgtt ccctacaaaa tcgttgaaga agacacctac tccaccgtca
     5461 aacgcgtatt agacgaaggc aaaacgactt gttcgctgtg cagccgcctg cgccgcggca
     5521 tcctctaccg cactgcaaaa gaattgggct gcaccaaaat cgccttggga caccaccgcg
     5581 acgacatcct cgccaccttg tttttaaata tgttttacgg cggcaaactc aaagccatgc
     5641 cgcccaagct ggtgagcgac aacggcgaac acatcgtcat ccgcccgctg gcgtatgtga
     5701 aagaaaaaga cttaatcaaa tacgccgaat taaaacaatt ccccatcatc ccgtgcaacc
     5761 tctgcggctc gcaacccaac ctgcaacgcc aagtcatcgg cgatatgctg cgcgattggg
     5821 acaaacgctt ccccggccgc atcgaatcga tgttctccgc cctgcaaaac gtcgtccctt
     5881 cccaccttgc cgataccgaa ctcttcgact ttgtcggatt ggaacgcggt caaagcctga
     5941 aacacggcgg cgatttggcg tttgacagcg aaaaaatgcc cgaacgtttt tcagacggca
     6001 gcgaagaaga cgagagcgaa atcaaaatcg agccgcaaaa agccgaacgc aaagtcatca
     6061 atattctggc gaacaagccg aaaacctgcg gctcatagac ctccgccgtc aaaaatgccg
     6121 tctgaagccc gtttgg
//
LOCUS       DAWHRS010000125         6084 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWHRS010000125 DAWHRS010000000
VERSION     DAWHRS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.125"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            486..5933
                     /gene="iga"
                     /locus_tag="WIB82_001693"
     CDS             486..5933
                     /gene="iga"
                     /locus_tag="WIB82_001693"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ3842966.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGANN
                     DITWLGAGIDVADSKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTSHASTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRRP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISSFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGANLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSAQKDAHFSKNNEVVFEDDWINRTFKATEITVNQSASFSSGRNVS
                     NITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSHLSVSLANHHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQ
                     QAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQK
                     VEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQS
                     QPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELL
                     AKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQAL
                     AARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAEL
                     ARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDY
                     MAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDEN
                     VSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHE
                     KVETVSLQPRAAQPRAQAATQPQAQAAAQADAVSTNTNSALSDAMASTQSILLDTGAS
                     LTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQI
                     GGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKA
                     NFNRASIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLT
                     AGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALLY
                     RNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         2045 a         1468 c         1359 g         1212 t
ORIGIN      
        1 agaatctcag actttcagat aatctttgaa tattgctgtt gttctaaggt ctagattccc
       61 gcctgcgcgg gaatgacgaa tccatccgta cggaaacctg caccgcgtca ttcccacgaa
      121 cccccatctc gtcattacca cgaaagcggg aatctaggac gcagggttaa gaaaacctac
      181 atcccgtcat tccctcaaaa acagaaaacc aaaatcagaa acctaaaatc ccgtcattcc
      241 cgcacaagcg ggaatccaaa aacaaaaagc cgcagaaatt taacggaaga accgaacccc
      301 gaaaaccggc ttcccgcctt cgcttgaata acagaacaaa ggaagcggaa ataaaaacat
      361 cgggattctt tttttaaatt ggcaataaaa caccaaaatg aatgatttac acaacaaaaa
      421 ttagtcaaca ccgccaaacc ggcgtaaaat acaaaacatt atcaccctta aaacggtaaa
      481 accttatgaa aaccaaacgt tttaaaatta acgccatatc cttatccatc tttcttgcct
      541 atgcccttac gccatactca gaagcagcat tggtcagaga cgatgtcgat tatcaaatat
      601 tccgtgactt tgcagaaaac aaaggcaaat tttttgtcgg cgcaaccgat ttatcagtga
      661 aaaacaaaca aggtcaaaac atcggcaacg cattatccaa cgtaccgatg attgatttta
      721 gcgttgcaga tgtcaacaga cgtacattaa ccgtcatcga tccccagtat gccgtcagcg
      781 tcaaacatgt aaaaggagac gaaatctctt attacgggca tcacaatggg cacttagatg
      841 ttagcaatga tgaaaacgaa tatcgttcag ttgcacaaaa tgactacgaa ccaaataaaa
      901 attggcatca cggtaatcaa ggtcgtcttg aagactacaa catggcacgc cttaataaat
      961 tcgtaacaga agtcgcacct attgcaccaa ccagtgcagg cggcggcgtt gaaacctata
     1021 aagataaaaa ccgtttttct gagtttgtgc gagttggagc aggtacgcaa tttgaatata
     1081 acagccgcta caatatgaca gagctttcac gagcttatcg ttatgccatt gcaggtacgc
     1141 cttatcaaga cgtgaatgtc acatcgaatc taaatcaaga aggcttgatt ggcttcggtg
     1201 ataattcaaa acatcattcc ccagaaaaac tcaaagaagt tttatctcaa aacgcattga
     1261 ctaactatgc tgtgttaggc gatagcggtt caccattatt tgcttatgat aaacaagaaa
     1321 aacgctgggt ctttttaggt gcttacgatt attgggcagg ctatcaaaaa aactcttggc
     1381 aagaatggaa tatctataaa aaagaatttg cagataaaat caaacaacgc gataacgccg
     1441 gcaccatcaa aggttatggc gaacatcatt ggaaaaccac gggcacaaac agccatatcg
     1501 gttcgacagc ggtaaggctt gccggcaatg aaaggggtgc aaacaacgga cagaatgtta
     1561 cctttgaaaa caacggcact ttggtattgg atcaaaacat caaccaaggc gcgggcggtc
     1621 tgtttttcaa aggcgattac acagtcaaag gtgcaaataa tgacatcact tggctaggtg
     1681 cggggattga tgttgccgac agcaaaaaag tcgtttggca agtcaaaaat ccgaatggcg
     1741 acagattggc aaaaatcggc aaaggcacat tagaaataaa cggcacaggc gttaaccaag
     1801 ggcaattaaa agtcggcgac ggtacggtta ttctgaatca acaagccgat gccgacaaaa
     1861 aagtccaggc tttctcccaa gtcggcattg tgagcggacg cggtacattg gtattaaata
     1921 gttcaaatca gattaatccc gataacttat atttcggttt ccgtggcggt cgtttggatg
     1981 ccaatggcaa tgacttgact tttgaacaca tccgcaacgt ggatgaaggc gcgcgcattg
     2041 tcaaccacaa cacaagccac gcctccacaa tcacgctaac gggtaaatct ttaattacca
     2101 atccgaacag cctttcggta catagcatac aaaatgacta tgacgaagat gattatagtt
     2161 attactaccg accaaggcgc ccgattccgc aaggcaaaga cctttattac aaaaactatc
     2221 gttattacgc cctaaaatcc ggcggcagcg tgaacgcacc gatgcccgaa aacggacaaa
     2281 cggaaaataa cgactggatt ttaatgggca gcacacaaga agaagccaag aaaaacgcga
     2341 tgaaccacaa aaacaatcag cgtattagca gttttagcgg tttctttggc gaagaaaacg
     2401 gaaaaggaca taacggcgca ttaaacctta atttcaatgg caaaagcgcg caaaaccgtt
     2461 tcttgttaac aggcggtgcc aatttaaacg gaaaaataag cgtaacccaa ggcaatgtct
     2521 tgttatcagg tcgtccaaca ccacacgcaa gagattttgt gaacaaatct tcagcccaaa
     2581 aagacgcaca tttctccaaa aacaatgaag tcgtatttga agacgactgg ataaaccgca
     2641 cattcaaagc cacagaaatt acggttaatc aatccgcatc attctcttcc ggcagaaatg
     2701 tatccaacat taccgccaac atcaccgcaa cagacaacgc caaagtaaat ttgggttaca
     2761 aaaacggcga tgaggtttgc gtgcgctcgg actataccgg ttatgttacc tgcaacacag
     2821 gcaacttatc cgataaggct ttaaacagct ttggagcgac acagattaac ggcaatgtga
     2881 atctgaacca gaatgcggca ttggtcttgg gcaaggctgc attatggggg caaattcaag
     2941 ggcaaggaaa cagccgtgtc agcctaaacc aacatagcaa atggcatttg actggcgaca
     3001 gccaagtaca caatctgtca ttggcggata gccatattca tttgaacaat gcctccgatg
     3061 cgcaaagtgc aaatcaatac cacacgctca aaatcaatca tttatccggt aacgggcatt
     3121 ttcattatct gacgcacttg gcggaaaatc ttggggataa agtgcttgtg aaggaatccg
     3181 catccggtca ttatcagctc catgttcaag ataaaacagg cgaacccaat caggaagggc
     3241 tgaatctctt tgatgcatca tccgtacgag accgttctca cctttccgtt tccttggcga
     3301 atcatcacgt cgatttaggc gcattgcgtt atacaatcaa aacagaaaat ggcattaccc
     3361 gattgtacaa tccttatgcc gagaaccgcc gccgagtcaa accggccccg tctcctgcca
     3421 caaacacggc ttctcaagca caaaaggcaa cacaaacgga cggtgcacaa attgccaagc
     3481 ctcaaaatat cgtcgtcgca ccgcctagcc cgcaggcaaa tcaagccgaa gaagcaaaac
     3541 gccagcaagc aaaagcggag caagtgaagc gtcagcaagc agaagcagag cgtaaatcgg
     3601 cagagttagc aaaacaaaaa gcagaagccg aacgcgaagc aagagagctg gcgacccgcc
     3661 aaaaagcaga acaagaacgc agcagcgccg aacttgcacg tcgacatgaa aaagaacgcg
     3721 aagcggcaga gttgtcggca aaacaaaaag tcgaagccga acgtgaggct caagcattgg
     3781 cggtacgccg aaaagcagaa gccgaagagg caaaacgcca agctgcagag cttgcacgcc
     3841 ggcatgaaaa agaacgcgaa gcggcagagt tgtcggcaaa acaaagggtc ggggaagagg
     3901 aacgccgtca aacagcccaa tcccagccgc aacgccgcaa acgccgtgcc gcaccgcagg
     3961 attatatggc agcttcccaa gaccgtccta aacgccgcgg acaccgttcg gtacagcaaa
     4021 acaatgtgga aattgcccaa gcccaagcag aacttgcgcg ccgacagcag gaagaacgca
     4081 aagcggcaga gttgttggca aaacaaaggg cagaagccga acgtgaagct caagcattgg
     4141 ctgcgcgccg aaaagcagaa gccgaagagg caaaacgcca agctgccgaa cttgcacatc
     4201 gccaggaagc agaacgcaaa gcggcagaat tgtcggcaaa ccaaaaagcc gcagctgaag
     4261 cccaagcatt ggcggcacgc caacaaaaag cccttgcgcg tcaacaggaa gaagcacgaa
     4321 aagctgcaga gttagccgtc aaacaaaaag cggaaaccga acgcaaaacc gccgaacttg
     4381 ccaagcaaag ggcagcagcc gaagcagcaa aacgccagca agaagctcgc cagaccgccg
     4441 aacttgcacg tcgtcaagaa gcggaacgcc aagcagcaga gttgtcggca aaacaaaaag
     4501 ccgaaacaga tcgagaagcg gcagaatcgg cgaaacgaaa agctgaggaa gaagagcacc
     4561 gtcaagcagc ccaatcccag ccgcaacgcc gaaaacgccg tgccgcacct caggattata
     4621 tggcagcttc ccaaaatcgt ccgaaacgtc gcggacgcag atctactctg ccggcaccgc
     4681 cctcgccatc atttgattcg tcagcttacg cagcacccag ggccttgcat aatccggact
     4741 ggtatgaaaa tgactatgaa gaaatcccct tggatgcgtt ggaagatgaa aatgtatctg
     4801 aatcagttga cacatcagac aaacagcctc aagacaatac ggaacttcat gaaaaatatg
     4861 aaaatgatta tgaagaaatc cccttggatg cgttggaaga tgaagatgta tccgaatcgg
     4921 ttgacacatc agacaaacag cctcaagaca atacggaact tcatgaaaaa gttgagacag
     4981 tgagtttgca accaagagcc gcgcagccgc gagcccaagc cgccactcag ccgcaagccc
     5041 aagccgccgc gcaagccgat gcagtcagca ccaataccaa ctcggcttta tctgacgcaa
     5101 tggcaagcac gcaatctatc ttgttggata caggtgcttc attaacacgg cacattgcac
     5161 aaaaatcacg cgctgatgcc gaaaaaaaca gtgtttggat gtcgaacacc ggttatggtc
     5221 gtgattatgc ttccgcacaa tatcgccggt ttagttcgaa acgcacgcaa acacaaatcg
     5281 gcattgaccg cagcttgtcc gaaaatatgc agataggcgg cgtattgact tactctgaca
     5341 gtcagcatac ttttgatcag gcgagcggca aaaatacttt tgtacaagcc aacctttatg
     5401 gtaagtatta tttaaatgat gcttggtatg tggccggcga tattggtgca ggcagcttga
     5461 gaagccggtt acaaacgcag caaaaagcaa actttaaccg agcaagcatc caaaccggcc
     5521 ttactttggg caatacgctg aaaatcaatc aattcgagat tgtccccagt gcggggatcc
     5581 gttacagccg cctgtcatct gcagattaca agttgggtaa cgacagtgtt aaagtaagtt
     5641 ctatgtcagt gaaaacacta acggccggac tggattttgc ttatcggttt aaagtcggca
     5701 accttaccgt aaaacccttg ttatctgcag cttactttgc caattatggc aaaggcggcg
     5761 tgaatgtggg cggtaattcc ttcgtctata aagcagataa tcaacagcaa tattcagcag
     5821 gtgccgcgtt actgtaccgt aatgttacat taaacgtaaa tggcagtatt acaaaaggaa
     5881 aacaattgga aaaacaaaaa tccggacaaa ttaaaataca gattcgtttc taaaatacta
     5941 aattcatagc aaaataaaat gccgtctgaa ctcaggcttc agacggcatt tttatagtgg
     6001 attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt acggcaaggc
     6061 gaggcaacgc cgtactggtt taaa
//
LOCUS       DAWHRS010000126         6059 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWHRS010000126 DAWHRS010000000
VERSION     DAWHRS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.126"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..388)
                     /gene="mafB"
                     /locus_tag="WIB82_001694"
     CDS             complement(<1..388)
                     /gene="mafB"
                     /locus_tag="WIB82_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ3842967.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVSPVLPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVY"
     gene            complement(428..1390)
                     /gene="mafA"
                     /locus_tag="WIB82_001695"
     CDS             complement(428..1390)
                     /gene="mafA"
                     /locus_tag="WIB82_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ3842968.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(1574..2293)
                     /locus_tag="WIB82_001696"
     CDS             complement(1574..2293)
                     /locus_tag="WIB82_001696"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ3842969.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVITGEDEGTLVHC"
     gene            2386..2493
                     /locus_tag="WIB82_001697"
                     /pseudo
     CDS             2386..2493
                     /locus_tag="WIB82_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2615..3469)
                     /locus_tag="WIB82_001698"
     CDS             complement(2615..3469)
                     /locus_tag="WIB82_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ3842970.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(3598..4326)
                     /gene="rpsB"
                     /locus_tag="WIB82_001699"
     CDS             complement(3598..4326)
                     /gene="rpsB"
                     /locus_tag="WIB82_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ3842971.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(4503..4736)
                     /locus_tag="WIB82_001700"
     CDS             complement(4503..4736)
                     /locus_tag="WIB82_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3842972.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            4814..5395
                     /locus_tag="WIB82_001701"
     CDS             4814..5395
                     /locus_tag="WIB82_001701"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ3842973.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            5456..6046
                     /locus_tag="WIB82_001702"
     CDS             5456..6046
                     /locus_tag="WIB82_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3842974.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHEMGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1363 a         1403 c         1673 g         1620 t
ORIGIN      
        1 ggtaaacagt aaaaccgccg tctacaccgc cgctgaaatc agaagtgctt tttgaatcat
       61 gatgatcgaa cggactgtgt acttcatgtc cgtgccctga aaaatgggtt tcataaccga
      121 taacaccttc aaaccctgtc cgttcgtgtg taataggcag tacaggactg accgcagttg
      181 catcaaatgt ctggacggcg taaacccgat ttttaacact gccgcgacca tcgccgaaca
      241 gatggtattt gccacccggt tcgtagtgtt ttgcctgcat atcatcgcgc aaacgggcat
      301 ccaaaccgtt cgcatgacta atggggattt gcagcaacga tattgctgct gcaaacagca
      361 tcatgaattt ttgaataggc aaattcattt tgttttcctt ggtagcaagc ggttatggta
      421 gtgtgaatca aggttgccct tgtctatgtt gtcgcactac ttcatcgctg tatccatacc
      481 cctcatgact gttatcagcc tctacggatg gggcagagtt acccgtatga ttgccgtatg
      541 gtcggatatc ggagaaatcg accattaatc cttccgtcgg tttaattcct ttgcttactt
      601 tatacggccc catccacaat gcgtaatttt ctttataggc agcttcaaac gcattggttt
      661 tgggtttgat gagcaatttt ttattggttc tgtctactgc gaaatattcc agttttgttt
      721 gggctttcag tgtttcggca ttgtataggt gcatttcggt tctgttgcgt atcgttccga
      781 atacgtcgat gttaataaac acatccgtat cggcattggc aggagaaaca acgtctatgc
      841 cgcgcaggaa aaatacggtc tgtaccaagt gggaaagaaa ggcagtgtcg cgcgggttag
      901 tcgtcaaggt ttcatttcga taatccccca tcccgccaat atttaagccc agactgcttt
      961 tacttccgct accgtctgat tgggtgcgcg agagtgcagg ggcattaagt gtagataaag
     1021 aagtggttaa acctgtcaaa ccgcctgatg ttgtttcagc ggtggtttcg taacgtggat
     1081 aggtgtaatc ggtacggacg gcagggctgt ttatgtattc gccacgaatc agtgcatcaa
     1141 tggagtagcg accccctgtc aaactgcctg aaccttggtc gcccatagtt gcaatgtaca
     1201 atgcaacttt tcgtccgtgt aatgcctgta aatccatgtc tttaacggca gctctggcag
     1261 aagcggccac aagttcttgt tcgaccgcaa agcgtttacc tccgccatgc gatggaatac
     1321 ctgtcagtgt cccgcaggcg gataaaataa aaactgaaaa aagaataggt atcagcagcc
     1381 gtgcttgcat agattttctc ctttgatgaa aaacaaattg tatcaaaatt gtaaatatag
     1441 tggattaaat ttaaaccagt acggcaaggc gaggcaacgc cgtactggtt taaatttaat
     1501 ccactataat ctccatataa taaatggctg tctgaagccc atatgcgact atatctgccg
     1561 acactatggt caatcagcag tgaaccagcg ttccctcgtc ttcgccggta atgacgcgtt
     1621 tgagcgagcc ttctttggcg atgccgaaga cgacgatatt gagcttgcgt tcgcggcaga
     1681 gggcgaaagc ggtcgcgtcc atgactttga ggtttttcaa caaggcttcg tcaaaagtaa
     1741 tggtttcgta gcgcgtggcg gacgggtctt ttttcgggtc tgcggtgtac acaccgtcga
     1801 cgttggtggc tttgagcatc acgtcgcagt tcatttccgc accgcgcaat gcggcggcag
     1861 tgtcggtcgt gaagaacggg ttaccggtac cggcggcaaa aatcacgact ttgccttctt
     1921 ccaaatattg gatggctttg gggcgggcgt aggtttcagc gatttgctgc atagacagtg
     1981 cggattgtac gcgcgctttg atgcctaaag tttcaaatgc gtctttgagt gccaacgcgt
     2041 tcatcacggt cgccatcatg cccatgtagt cggcggtggc gcgatccatg ctgcctgctt
     2101 gggcggatac gccccggaaa atattgccgc cgccgacaac aataccgact tgcacgccca
     2161 ttttaacgac ttcggcaatt tcgccgacag tttgaacgat ggtatcgtga ttgatgccga
     2221 acggatcgga acccatcagg gattcgccgg agagtttcag taatacgcgt ttgtatttga
     2281 tttgctgtgt catggatacc ttgctttctt gaatgtcgtc ggtcggagtt gggtcgtctg
     2341 aatttcaaac ggtcggaatt acaatgatat ggcgtgtcgt tctgttatag tggattaaat
     2401 ttaaattagg acaaggcgac gaagccgcag acagtacaaa tagtacggca aggcgaggca
     2461 acgctgtact ggtttaaatt taatccacta tattgttttg ttttcagacg gcctttatat
     2521 tcggtcgtct taaaataggt accgtaaacg gttttctcaa aaaaaagcac cctgattcgt
     2581 ttggaatcca ggtgctttct ttttcataag tgccttacac tttggcggct gcggctactt
     2641 cggctgcgta gtcgacaacg gctttctcga taccgtcgcc tactttgtag cggacgaagc
     2701 tgaccacttc agtgccgttt tctttggcga attgggcaac agtttgatcg gggttcatca
     2761 cgaatgcttg gccgttcaga gtgatttcag ccaagaattt gcggatacgg ccttcaacca
     2821 ttttagcggc gatgtcggca ggtttgccgg aagcgatggc ttgttcggtg tagatgtggc
     2881 gttctttttc aacggtttcg gcatctactt cggcttcgct tacgcattgt ggtttagcgg
     2941 caacaatgtg cataccgatt ttgcgtgcta cgtcttcaga gcctttgtac tcaaccaata
     3001 cgccttcggt cgccaatgcg ccgtggatgt aggcaaccag ttggttggca gtgtcgatca
     3061 cttggaagcg gcggacagac atgttctcgc ccaatttggc gatgatggct ttgcgttctg
     3121 cttcaaccag ttcgctcagt tcttcaacag aagccggttt tttctcggca gcagttttcg
     3181 caacgaagtt ggcaaattct acgaagcccg cgtctttagc aacgaagtcg gtttcgcagt
     3241 ttacttcgac caatgcgccg acattgccgt tgatcgcgta agccaatacg ccttcggcag
     3301 cggtacggcc ggccagttta ccggctttcg caccggattt gatacgcagg atttcttcgg
     3361 ctttgtcgaa gttgccttcg gcttcaacca aggctttttt gcattccatc atgcccaggc
     3421 cggtagcggc gcgcaggtcg gcaaccattt ttgcagtaat ttctgccatt ttgaatctcc
     3481 tagatttttg ggaaatacgg aaaggatttt ccgcattcgg gaatcgtgtg ccgcgaacgg
     3541 acgttcagac ggcatatttg agaaaagggg cataacgccc ctcttcggtt tgccggatta
     3601 ctcggcagcg gcttcttggg cagcggctac ggtttcttgc agcgcttggt ttttgccttc
     3661 caaaactgcg tcagcgatgc cgcggcagta caggcggatg gctttggcgg agtcgtcgtt
     3721 gccggggatg acgtatttca cgccgtcagg gctgttgttg gtatcgacta cggcgataac
     3781 ggggatgccc agtttttcag cctcgaccag agtacctttt tggtagccgg tatcgataac
     3841 gaaaatcgcg tcgggcaggc ctttcatgtt tttgataccg cccaaagaac gttccagttt
     3901 ttcaacatcg cgttgcattt ccagaatttc ttttttgctg aaaccgcttt cggcagcatt
     3961 ttccaaggct gcggtttttt cttccaggcg tttgatggat tgcttaacgg ttttgtagtt
     4021 ggtcagcata ccgcccaacc agcggtaatc gacgaaaggc ataccggcgc gggtcgcttc
     4081 ttcgcggatg atgtcgcgtg cctggcgttt ggtacctacg aacaatactg tacctttgtt
     4141 ggcaaccaaa cgacgtacgg cttcttgcgc gtcttggaac atcggcaggg ttttttccag
     4201 gttgacgata tggattttgt tgcgcgcacc gaaaatgtat tgagccattt tcgggttcca
     4261 gaaacgggtt tggtggccga agtgaacgcc ggcttcaatc atctgacgca tagtaatttg
     4321 agacattttg atttccttga aagggttaag ggcacacatt caatcgcata gaacttgata
     4381 ggcgatgcct attgtcaagc acccttcggc gaaagtgggc ataagtttta aaaaataaaa
     4441 gagtgtttcc gaaatggaaa actgacggat tatagcggat aagaggccgt ctgaaaaggg
     4501 attcagggtt ttgtgccgct gtctttttgc cgttgtgcac gctgctgttc gaattctgcc
     4561 tgttcctgtt gcttcatcaa gcggttgcgg cggcggacgg ttacgacgaa gaccgtgaac
     4621 acggtgggca gcaccgccca aaaaaccaaa taaatcaagg cgcgcgcaat actcggctgc
     4681 gcggcggaat acatcacggc aacaaaaaga tagccgatgg cgacaatgtg ccacattgtg
     4741 tgcgtcctgt ttgaatgtta acaaaatcgt tataatcgca acattctacc gcaatcctga
     4801 aaggccgtct gaaatgagga acgaggaaaa acacgccctg cgccgcgaat tacgcgggcg
     4861 gcgttcgcaa atggggtggg acgtgcgggc ggcggcaacg gtaaaaatca accgcctgct
     4921 caaacgttat atcaagcacg gtcggaaaat cggcgtgtat tggccgatgg gcaaggaatt
     4981 gcgtttggac ggctttgtcc gcgcggcgca aaaacgcggc gcaaaacttt atctgcctta
     5041 tatcgaaccg cgttcgcggc ggatgtggtt tacgccgtat cctgaaagcg gaatggaacg
     5101 ggagcgcata cggggcaggg cgaagttgaa cgtgccgcag tttgcagggc gcaaaatccg
     5161 cgtgcacggt ttgtcggtat tgctcgtccc gcttgtcggc atagaccgcg aaggctaccg
     5221 cttggggcag gcaggcggct attacgatgc gacgcttgca gcgatgaaat accgtttgca
     5281 ggcaaaaacc gtgggcgtgg gctttgcctg ccaactggtc ggcacgctgc cgcgcgaggc
     5341 gcacgacctg ccgctggacg gttttgtgtc ggaggcgggg atattgtgtt tttagccgtt
     5401 cagacggcat tccgggcgga aatgccgtct gaaggcggaa tattgggaga aaaggatgtc
     5461 ggaaaggata atactgccgg tcttgtcgtt gggcggcgtt cgtctcgaac cgttggatgt
     5521 gcatcatgaa atgggtttgc gcgaggcggt ttgcgatggg gaagtttgga agctgggagt
     5581 aacgtccgcg ccccatccgg atagggtggc agactatatc gggacagcat tggcaacgcg
     5641 tttggcgttt gcggttgtcg atgaagaggc gggcagggtg gtcgggacaa cggcgtatta
     5701 tcattttgaa ccgcagattc cgcgtttgga tatcggattc acatggtatg cggcatcggc
     5761 acggcggaca cggataaaca cctgttgcaa aatcatgctg ttggattacg catttgatgt
     5821 tttggcttgc cgttgcgtgg gatggcgcac ggatattctg aaccttgcct cgcaacgtgc
     5881 catcgaacgg ctgggtgcgg aaaaagacgg cgtgctgcgt atgcatatgc tccgtaaaga
     5941 cggcagtgtg cgcgatacgg tcgtgtacag tatgctgagg gaagattggt gcaaaaacag
     6001 ggaaatcctg accggcaggt tggcagggta tggcgtgcag gtgtgaggca gtattttca
//
LOCUS       DAWHRS010000127         6023 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWHRS010000127 DAWHRS010000000
VERSION     DAWHRS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.127"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(56..1459)
                     /gene="mtrE"
                     /locus_tag="WIB82_001703"
     CDS             complement(56..1459)
                     /gene="mtrE"
                     /locus_tag="WIB82_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ3842975.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAALNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1514..4717)
                     /gene="mtrD"
                     /locus_tag="WIB82_001704"
     CDS             complement(1514..4717)
                     /gene="mtrD"
                     /locus_tag="WIB82_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ3842976.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4729..5967)
                     /gene="mtrC"
                     /locus_tag="WIB82_001705"
     CDS             complement(4729..5967)
                     /gene="mtrC"
                     /locus_tag="WIB82_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ3842977.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAEQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPVVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIASITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1352 a         1709 c         1622 g         1340 t
ORIGIN      
        1 aagaaatgcc gtctgaaacc ttatcgtttc agacggcatt ttattgcccg accggttatt
       61 tgtcggtttg ggtatcccgt ttcaatccgc cgccgagtgc cttgtacaaa tcggcaaggt
      121 tttcggcgcg ggtcagttgt gccgacaaag ccgcaccctc cgccgcatag ctgctgcgtt
      181 ccgcatcgag caaatcgagc gcgccggata cgccgtgctt gtaacgcagg ccgaccaagc
      241 gcaacgcttc tttagaggcg cggctttgtt tgcttaaagc gtcataggct ttatccagct
      301 gctcgcgcgc cgccaatgcg tttgccacgt cttgaaatgc ggattggacg gcggattcat
      361 aggcaacgat ttgtgcctgt tggcgcagct tggctacatc aaggttcgcc ttgttcgtac
      421 cccaggtaaa aatcggcagg gtaatagacg gcgcgaacga ccaaacgccc gtgccgcttt
      481 tgaacaaccc acccaattcg gcagaacccg taccgacggt tccggtcagg cggatggatg
      541 ggaaaaaggc ggcgcgtgcc gcaccgatat tggcgtttgc ctgtttgagc gcgtgttcgg
      601 cagcacggat atcgggacgg tcgagcaata cttcggaact caaaccggcc ggcagttttt
      661 caacaaaaaa ctgcttgtcc agcggcaaac cggcaggcag gtcttcgggt atcggttggt
      721 taatcaaggt tgccaaggca ttgcgcgcct gttcgcggct gcgcgcggca tgggcataat
      781 cggctttggc agattcgatc agggcttcct gctgacgtag ggcgacggcg gaaatcacgc
      841 ctgccttgta acgtaattcg gacagcttgt aggtttcctc gcgcgttttc aaaacacgtt
      901 gcgccaaaga catcgcttct tcggcgtaac gttcgttgaa ataggctttg gcaacggtgg
      961 caatcaggct caaatgtgcc gcatcgcggt tggcggtgct ggcgaaatag ccttgcagtg
     1021 ccgcctcgct gctgctgcgt acacgcccga acagatcgag ttcgtaagat gccgcaccca
     1081 gtccgacttt gtagctgctg cttacattgc cgccgctcaa gctgccttgg cgcgagtcgt
     1141 tcgcattggc ggcaagcgtg ggcaggaggt tgttgcgctc aatcatgtat tgtttgcggt
     1201 agatttcgct gttcaatgcg gcggtacgca aactggtatt gcgctcgagt gcgatgtcga
     1261 tcagcttttg caggcgcggg tcggcaaaat agtcatgcca acctaaatcg accgcacgga
     1321 tgccgctgtc ggcggtatcg tttttgaacg tttcggcaac ttcgactttg ggctgctcgt
     1381 attggggaat catggtgcag gcggataatg cgaaggctgc tgcaacagaa gtcaaggtgg
     1441 ttttcaatgt agtatccata aaaaagtcct gatgccgtct gaaaacccgt gggcgttcag
     1501 acggcatggt tgcttaatgt tggctgtcgt ccgaaccggt gatgcccgct tcggcggcgt
     1561 gttttactgc catttcgtgt tcgtgcgcgg tttctttgaa gaatttgcgc accaccacat
     1621 agaaaagcgg aacaaggaac acggacaaga gcgtgccgat gagcatcccc cagaatacgg
     1681 tcgtgccgat ggcgcgctgg ctggcagaac ttgcaccacc ggcaatatac aggggaacca
     1741 cgcccaaaat aaaggcgaac gaggtcatga taatcggacg gaaacgcagg cgggcggctt
     1801 ccaaagcggc ttcgaccgcg cttttccctt gcgcttgaag gtctttggca aactcgataa
     1861 tcaaaatcgc gtttttcgca ctcaaaccca tcacggtaac gaaaccgact tgaaagtaga
     1921 tgtcgttggc gaacgaggga acgctgccca acagtccttc aaacaggttg cgcccggtta
     1981 cgcccgcagc cgcaccgatc aaacccaacg gaatcacaag gatgaccgcc agcggaatcg
     2041 accagctttc ataaagcgcg gcaagtacca aaaatacggc tgcaaccgcc aaaccgtaca
     2101 aaatcagggt ttgcgagccg ccttttgcct cttcgcgcga ctgtccgccc cattcgaagc
     2161 tgtaaccgcc gcccaattcg tcaaccattt tttgaaccgc cgccatagcc tgcccggtgg
     2221 aaacgccggt tgcaggcgaa gcggacagct tcatcgaagg ataaccgttg aagcgtacgc
     2281 tctgttccgt accgttttcc caagaaacag tagcaatggt ggaaagcggt acggcgacgc
     2341 cggatttgtt cggcacggtc aggttcaaaa tatcggcagg ctgcatacgg gcatcctcgt
     2401 cggcctgcac catcacgcgt tgcagacggc cttggttcgg gaagtcgctg acataagacg
     2461 aactcagcgc acttgccaat gcggtgcgga tgtcggcaaa cgaaatgcct tgcgccgccg
     2521 cggcggcacg gttgatgtcg attttcaact gcggcgagtc ttccaaaccg ccagcacgga
     2581 cggtgctggg gtcaaacaaa ccgctggcac gcattttctg aatcaactcg ttgcgcttcg
     2641 ccagcaatgc ggtatggccg gtattattgc ggtcttgcag gttgatgctc aggcccgaac
     2701 cattgcccaa ctccagaatc ggaggcggga cgacggcgat gccaaaaccg tctttaagcg
     2761 tccccatcat catacccgtc agcttgccgg caatcgcaac ggcatcgctg ccgggcgcgg
     2821 tacgctcgtt ccaatctttc aatatggcaa aacccatcgc catattctga ccgctgcccg
     2881 aaaagctgaa gccggaaacg gtaatgatgt tttctatttc aggaatgctt ttcgccagtt
     2941 gggtaacttg cgccaaagtc gcattggtgc gctcttgggt cgctcctgca ggcagttgca
     3001 cgctgaccat gacgaagcct tggtcttcgg tcggcaggaa tgaagtcggc aggcgcataa
     3061 acaggaacac gcccacaacc gccaagccga tatagacaac catcatgcgg aaagtcttac
     3121 gcagcacttt ggcaacccgg ccttcgtaac cgtgcgtcca actgttgaat ttcttgttaa
     3181 accagccgaa gaaacctttt ttctcttcgt gatgcccttt cgggattgtc ttcaacattg
     3241 tggcacacaa agcaggggta agggtcagcg caaggaaggc ggagaatgcg attgatgacg
     3301 ccatcgtcag ggcaaactgt ttgtaaatat tgcccgtcgc cccgctgaac atcgccaacg
     3361 gtacgaacac ggaaatcaga acggcggtaa taccgatgac cgcgcccgaa atctgaccca
     3421 tcgctttttt ggtcgcttct ttgggcggca agccttcacc cgccataatg cgctcgacgt
     3481 tttcaaccac cacaatcgcg tcatcgacca cgatgccgat gaccaaaacc atcgcaaaca
     3541 tggtcagtac gttaatcgac atgcccatat aagagatgaa ggcgaaaccg cccaacagcg
     3601 aaatcggtac gacgatggtc ggaatcagcg tataacggat gttttgcagg aagagataca
     3661 ttacgacaaa caccagcacc atcgcttcga ttaaagtgtg aatcactttt tcaatcgaaa
     3721 tttcgacgaa tttggaagta tcgtaagggg ttttccagct cataccctga ggaaagtatt
     3781 tttccaacgt cgccatgcgt tctttaaccg cctttgccgt cgccatcgca ttgccgctgt
     3841 tggacagcat caccgccata ccggtggtat ttacaccgtt cagacgggtt gaggaagaat
     3901 agtcttccat acccagtccg acccttgcca catccttcag gtaaacatta gaaccgtcgg
     3961 tattggcgcg gaggatgacg ttgccgaatt cttctgccgt acccaactgc ccttgcgccg
     4021 ttacggtagc cgtaaccgtc tgtccgcgaa cggcgggaag cgaaccgata gaacccgctg
     4081 aaatctggac gttctgggcg gacagcgcgc tgccgatatc ggcaaacgac aaattgtagt
     4141 tttgcagttt cttaggatca acccaaatcc gcatcgcgcg ttgcgcgccg aacaggcgta
     4201 cctgccccac gccttcgata cgctgcaact cgggaacgat attacgctgc gcgtagtcgt
     4261 tcatctcttc ggttgactgc acatccgacg aaagcatcac aatcatcagg aaattggaac
     4321 gcgccttgga tacggttacg ccgtattgct ggacggttgc cggcagcgtg ctcaatactt
     4381 cggaaagctt gttctgcact tccacctgcg ccagattctc gtcggtatcg ggcgtaaagg
     4441 tcaggctcac gctgccgctg ccgctcgaat cggcggaagt ggacatataa tccaaacctt
     4501 ccacgccgtt catattccgc tcgatcacgg aaagcacgct gtcttccatt acctgcgcgg
     4561 acgcacccgg ataagtggcg tgcaaagtaa tggtcggggc ggcgacagac ggatattgcg
     4621 aaacaggcag gcttttgatg ccgaaaatac ccgccgcaat aatgaaaatc gaaataaccc
     4681 acgcaaaaat agggcggtcg ataaaaaatt tagccatcga tgccttcctt attccgcttc
     4741 agaagcagtt ttggcttcag atgccgtctg aacgccggat tgaggcgcgg cggcttggtt
     4801 ttcagacggc gcccattctt tgggcgttac ctttttcgca cccgttatac tggcgatact
     4861 gatgccttcc acaaccacct tgtccccgtc cttcagaccc gacgtaacaa tccaattcgt
     4921 accctgctgt tgcgcaaccg ttacctcgcg gggttccata ccgccttggg cattcacaat
     4981 catcacggta tctttcgcac cgcgcgttac cgcctgctgc ggcacaacaa atgcgttatc
     5041 caccgccact tggtccatca gcacgcgcac atacagaccg ggcatcagga tattctgatc
     5101 gttcggtacg gcggcgcgca gggtaatctg accggtcgat tcgttgacga ccggatcggc
     5161 aaacagcagg cggccttttt cagggtaaac tgtgccgtcg tcaaatttga tgccgaccgc
     5221 aatcacacca tccgccgcca gcagtttgcc ttcggctatc tgacggcgca atttcatcac
     5281 ttcggatgca gactgggtaa cgttcacata catcggattg gtttggcgga tggttgccag
     5341 cacggtcgta tcgcccgcat tcaacagcgt accttcggaa actttagact gaccgataaa
     5401 gccggaaatc ggcgcggtaa tgcgcgaacg gttcagattg atgccggcag atttgatcgc
     5461 cgcctgtgct gctttgacac ctgcctcggc agaacgtttc gccgttaccg cagcatcgta
     5521 ttcctgccgg ctgacggctt cggcggcaac caaaggcttg tatcgcgcca aatccgcatc
     5581 cgctttggca agcgttgcct gagccgttgc cagttgcgcg cgcgcgcttt ccagacctgc
     5641 ttcataagtg gaactgtcga tctgatacag cggctgttcg gcacggacat aactgccttc
     5701 ttggaacagg cgtttttgga tgatgccgcc gacttgggcg cggacatcgg cggtacgcag
     5761 cgattccaaa cgccccggca actcgacggt caacgcgacg gtttgcggat ggacggttac
     5821 gacaccgacg acgggcgcag gggcttcccg accagcaggc tgcccgccct gcgccgcgtc
     5881 tccgccttta ccgcaagacg acagtaccaa tgcaacggcg gcagccaacg cggccgcacg
     5941 catcgcctta aaagcataaa aagccattat ttatcctatc tgtctggttt gatgtaaagg
     6001 gttttgccaa tcaacaggca ttc
//
LOCUS       DAWHRS010000128         5911 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWHRS010000128 DAWHRS010000000
VERSION     DAWHRS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.128"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1018)
                     /locus_tag="WIB82_001706"
     CDS             complement(<1..1018)
                     /locus_tag="WIB82_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ3842978.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAAPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSHMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEA
                     RISRL"
     gene            1183..1863
                     /locus_tag="WIB82_001707"
     CDS             1183..1863
                     /locus_tag="WIB82_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ3842979.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            1892..2251
                     /gene="crcB"
                     /locus_tag="WIB82_001708"
     CDS             1892..2251
                     /gene="crcB"
                     /locus_tag="WIB82_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ3842980.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASLSPATGNLFANWIGA
                     FLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQLNRPASALSNIFLHTV
                     GSLLLTWLGLKIGAAVK"
     gene            complement(2918..3454)
                     /locus_tag="WIB82_001709"
     CDS             complement(2918..3454)
                     /locus_tag="WIB82_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ3842981.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(3488..3844)
                     /gene="folB"
                     /locus_tag="WIB82_001710"
     CDS             complement(3488..3844)
                     /gene="folB"
                     /locus_tag="WIB82_001710"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ3842982.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            3900..4502
                     /gene="plsY"
                     /locus_tag="WIB82_001711"
     CDS             3900..4502
                     /gene="plsY"
                     /locus_tag="WIB82_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ3842983.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPVAASFF
                     MPHVSWVWATVAIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            4579..5424
                     /locus_tag="WIB82_001712"
     CDS             4579..5424
                     /locus_tag="WIB82_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ3842984.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKELISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            5670..>5911
                     /locus_tag="WIB82_001713"
     CDS             5670..>5911
                     /locus_tag="WIB82_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ3842985.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPA"
BASE COUNT         1358 a         1791 c         1321 g         1441 t
ORIGIN      
        1 tcaggcgcga aatccgcgcc tccgcagaaa ccgttgcctt gttcggttct ttcaaattga
       61 cggaaacgcg gcggcaggca tcgcggacgg ctgtgccgtc tgaaacggac tgcccttggg
      121 aaacatccgg ggattttgcc tccgcctgcc ctttcccctg ccctgcatga cggcggaacg
      181 gcgcattccg tgcattttcg gctttgactg cagaaaaggc agacggatgc tgcttctcaa
      241 aatacggctc aaatccgtaa tccgcagact cggaaaacgc ttctttgtcc gcatcgaaca
      301 tatgggaata cgtgcgttga agcacagggt cgtccaaacc gataatctgc agcccttcca
      361 tcggggtatc ggacacgcgg actttggggg ccgctgtttc gtttttctca aatgcatcga
      421 tatagcgttt tttcgtttcc cttaaagccg cgctgtttaa ggcacgcgtt gcttcttcca
      481 aagtgatttc tttaaaaacc ggacggacgg gcgaaatttc gctttcagac ggcatcaacc
      541 cttcagcaat actccggtcg aatgggatgc ggcggttgtc gattacggca gttgcaatgt
      601 cttccgtatc ggcagcctct tcctccgcag cttctgctgc ttccgtttcc gcttcttccg
      661 tcccgtttcc actgtctgaa tatccgtcgg atgcgttcgg aacatccgca cggtttgcag
      721 aagcaggcgg aacaggcctg tccggttcgg attcgtgcgc caaatagttt cggcagaaac
      781 ggacaacacc gacaaacagc caatacactg ccgttttgac ggcatggaaa agcatcaggg
      841 caagttcggg aaaatcgggc ataccgtctt tgatttcagg cagctccgcc tgtttttccc
      901 ctttcttttc ctgccacgca gaaacctcgc gcatccactc gcgttcggat tgtgcgcgga
      961 caaaaaacaa gccggcaagc gcaagcaaca aaataacgat caaaactatc caaaacatat
     1021 gccgtttccc gcaagacaaa cgattaaacg ggtttatttt aacgtgtttc catcccaaaa
     1081 aaaacaaccg gtcggcaatt ctgccgaaac tgcttcggct ttcggtttgc ccccgccgac
     1141 agtataatag ccgtcctttc ccttaatctt tgcggcattg ccatgatttt ccaaacagtc
     1201 tggttttcag atatggtact gtcggtttcg tggattgtcc ttatcctgat actggcagct
     1261 tccgcgccgt ccgcattccg ctcgcttgcc cgctaccgtt ctgcccttcc cctgtgtacc
     1321 gtaatttttt ccgcagcatg gtgtctgaac gcctctgccg gcggcggaca gcttgcccag
     1381 atgaactacc acctgctcgc cgtcaatctg gttgcactga tggtgggcac ttccgccgcc
     1441 ttttggcttg ccgcgctgct gatgctgccc tactgcctgc tgtttgccgg ttctgccggc
     1501 gcatatccgc cgaacgcttt agtgctgatt ctgcccgcgc ttgccgtcaa ccgcctgtcg
     1561 cgtatgctgg tcaaccgcct gccgcccaat attttcatct ttattttcgt caacggcttt
     1621 ctcgcttccg ccgccggcat tctgctgacc gggctggtgc tgaccggtat tttggatgcc
     1681 gcaaacgctt ttccgtccga aatattgtgg acgaccgccc tgcccgtctt tattctgctg
     1741 gcgtgggcgg aagcttttct cagcggcatt tcaactgcca tatttgttgc actgaagccc
     1801 cattggatca acactttcga cgacaaccgc tatctgaagt ccgaccgcgg catatggcgg
     1861 tagccttcag acggcataga caggcaaaac catgctttcc aacatcatcc ccctttccat
     1921 cggtgcggca ctcggtgcaa cggcgcgttg gctgctcaac ctcgccgttc ccgcatcgtt
     1981 gtctcccgcc acaggcaacc tgtttgccaa ctggataggc gcttttctta tcggaatctt
     2041 tgccgaaacc gtcaaccacc cgcaatggaa gctgctgctg attaccggtt ttctcggcag
     2101 cctgaccacg ctttccggat tttcactgga aaccgtaacc ctgctccaat tgaatcgtcc
     2161 cgcttcagca ctttccaata ttttcctgca tacggtgggt tcgctgctgc tgacttggct
     2221 cgggctgaag ataggggcgg cagtcaaata accgcaacaa attgcctccc tttgttttga
     2281 cataatctat aaattagctt cgcttgttta aatactggct gtgccaatta tagtagatta
     2341 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca
     2401 cttggtactt cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta
     2461 ctggtttttg ttaatccact ataccgttct caaattcaaa ttgggctttt aggtctataa
     2521 caaagaaaaa gccttggtag gataaaccta ccaaggcttt ttcttaaaaa aacgctctat
     2581 taccgtgcct aaaaatgcaa aaaaagtaac catcataacc cttttgccaa aattaatatc
     2641 atctattaat tcatcatgcc atgcattaat agatggtata taagaaagag ccaataaact
     2701 aataaaccaa taacaaaaat tagtaacaaa gatattcatt attaaccatt ttaaaaatta
     2761 tcatgttcgt ttgggtaatt atcataccct tttccctctt attactaaag cacgaatgac
     2821 tttgaaggac gcgcccagag acaagccgaa aaggcatatc tcatccgcca aacaaaatgc
     2881 ccgccgatct actccggcgg gcaattccaa catcctgtca atctttcatc agccagtatt
     2941 gtaaaccgat taatgtttta ccatctttaa tttcatcgtt tgccaatgcc tgacggactt
     3001 cttctttcga catcaatacg gtttccgtaa tctcgtcttc gtcattggca agcgtactgc
     3061 ccaaacgcac gccttccgct tcgaacagat acattttttc gttgcaaaaa ccgaccgccg
     3121 tataaaaact gtaaagcagg cgcacgctgt cggcggtata aggtgtttcc tccgccaatt
     3181 ctcgcagcgc acacgctgcc atatcctcgc ccgccacatc cagcttgccc gcaggaagtt
     3241 ccaatgtcgc ctgatttgcc gcataacgcc actgccgcac caaaaccact ttcccctcgt
     3301 ccgtaaccgc caacacgcat gccgcacccg gatggcggat gacgatacgc tgcccttcat
     3361 tgccgttggg caacctgacc ttatccctgc tgatactgac gaaaccgccc tcgtaaatgg
     3421 tttcgccgcc taattttact tccctcaaat ccacttcata tctcctttcc gtttcctatt
     3481 ctgccgttca atcttcacgc ttgccgcgct cgatttccac gccaacctcg cgcacgcctt
     3541 ccaaaatacc cggcttgacg attttcacgc gcacccacac cgcgccgaaa tatcccaaaa
     3601 ccaaatcggc aatatattcc gccaacgctt ccaaaagcag gaaatcctgt tctttcagat
     3661 gtcggcgcag cgtttcgcat acctcggcat aatgcaccgt attggcaata tcgtcgtccg
     3721 aacccgcttt ctcgggaaca ccgatgtcca aatcgacaat cagggtctgc aaccgttcgc
     3781 gttcccagcc gtacacgccg ataagcgtat ccgccttgat gccgtgcaaa aagattttat
     3841 ccataatccg ccccgttttt tgctaaaata accgcaccat tctaagtaaa gtaccgcaaa
     3901 tgttcaacat accggctgtt gctgtttcct atctgatcgg ctcactttct ttcgccgtca
     3961 tcgtgtccaa gtattacggc atggacgacc cgcgcaccta cggatcgggc aatccggggg
     4021 caaccaatgt tttacgcagc ggcaaaaaaa aggcggccgc gctgacgctc ttgggcgatg
     4081 ccgccaaagg tttagttgcc gttttgcttg cacgcgtgct tcaagaaccg ctcggtttat
     4141 ccgacagcgc aatcgcggcc gtcgcactcg ccgcgctggt cgggcatatg tggccggtgt
     4201 ttttcggatt taaaggcggc aaaggcgtgg caacggcatt gggcgtgctt ctggcactct
     4261 ctcccgcaac tgccttggtc tgcgcgttga tttggcttgt tatggcattc ggcttcaagg
     4321 tgtcctccct tgccgcatta accgccacaa tcgccgcacc ggtcgccgca tccttcttta
     4381 tgccgcacgt ctcgtgggtt tgggcgaccg tcgccattgc tttgctggtg ttgttccgcc
     4441 acaaaagtaa tatcgtcaag ctgctcgaag gcagagaaag caaaatcggc ggcagccgct
     4501 gatttcccat ccatccctgt ttatgccgtc tgaaccccgc ttcagacggc atccgaatat
     4561 cccgccgttt taccgactat gctcagttac agacacgcat tccacgccgg caaccacgcc
     4621 gatatgctca aacatttcac cttgttttta gtgctgcaat atttcaaccg caaagacaag
     4681 ccctactggt acatcgacac gcacggcggc gcgggtgtgt acaatctcga aggcagcgag
     4741 gcgcagaaag tcggcgaata ccgacaaggc atcgccctgc tccgacaggc gcaaaacctg
     4801 cctgccgaac tctctgactt tgccgcgcac atacaaaaaa tcctgccctc gcccgaactt
     4861 tactgcggtt ctccgtggct ggcgcaatcg ctgacccgcg tcggcgacaa attacgcctg
     4921 tttgagctgc accccaccga ctttgtccat ctgcaaaaca atatgggcga agcaggattg
     4981 ggcaaacgcg gacaagtgtt gcgcgaagac ggttacaaag agctgatttc cctgctgccg
     5041 cccccgccgc gccgcgccac cgtcctcatc gacccgccct atgaggaaaa acaggactac
     5101 cggcgtgtaa cggaaacgct gaaagccgct ttaaaacggt ttgagtccgg ctgttatctc
     5161 atttggtatc cctgcctcag ccgcgaagaa agccgcaagc tgcccgaaga attgaaaaaa
     5221 ctcctgcccg acaactacct tcacgccgaa cttcacgtcc acgcgccgaa agccgacggt
     5281 ttcggtatgc acggcagcgg aatgttcgtc atcaatccgc cctatctgtt ggcggaacaa
     5341 ttggcggcaa acctccccgc cctgacgcgg ctgctggcgc aagacgaagg cgcgcgctac
     5401 cttttggaca gcaaaatccg ctgaccccga tgccgtctga aacccttcag acaacatctc
     5461 ccgtcaaata aacaaaaccg gacgatatac cggcagtcgg cacttttaag aataaatatc
     5521 ccctcatccg cctgccgcgt tccgacttgc cccgcgccgt ttttttccac tacaatccca
     5581 cgcgatatct ggcggaacgc accaaaggaa cgccccgcag caatctgccg cctttcagac
     5641 ggcacaaacc tttccgaaac gagcaaacta tggacacact gacccgattc ctccccgaac
     5701 acctgcaaca aaaccagctg cccgaagcac tcggcggcgt tttactgtcc gttgtttccg
     5761 cctgcaccga aatcaacgcc aaagtccgcc tcggcgcact ggccggcgta ttgggtatgg
     5821 cgggcacagg caatattcag ggcgaagacc agaaaaaact ggatgttatc gccaacaaca
     5881 ttatgattga cacactcaaa gccaaccctg c
//
LOCUS       DAWHRS010000129         5906 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWHRS010000129 DAWHRS010000000
VERSION     DAWHRS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.129"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(98..1015)
                     /gene="thrB"
                     /locus_tag="WIB82_001714"
     CDS             complement(98..1015)
                     /gene="thrB"
                     /locus_tag="WIB82_001714"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ3842986.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="WIB82_001715"
     CDS             complement(1052..1780)
                     /gene="ubiG"
                     /locus_tag="WIB82_001715"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ3842987.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1932..3173
                     /locus_tag="WIB82_001716"
     CDS             1932..3173
                     /locus_tag="WIB82_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ3842988.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFAGSLIVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3306..3869
                     /gene="gmhB"
                     /locus_tag="WIB82_001717"
     CDS             3306..3869
                     /gene="gmhB"
                     /locus_tag="WIB82_001717"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ3842989.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            3898..4641
                     /locus_tag="WIB82_001718"
     CDS             3898..4641
                     /locus_tag="WIB82_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ3842990.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            4638..5330
                     /locus_tag="WIB82_001719"
     CDS             4638..5330
                     /locus_tag="WIB82_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ3842991.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
BASE COUNT         1393 a         1906 c         1398 g         1209 t
ORIGIN      
        1 aaaatacccg gctatcgtct cattttcgtt ttaatcagcc ataaaaatgc cgtctgaaac
       61 cctttcagac ggcatttctg tcaaacgccg gacgcactca atccaaactc aacagcaggt
      121 tgcggaacgc gttcgggtct ttgataaacg tcatctcgcc cgcctgcgga aaatgaaagt
      181 ccaacaggcg cgacacccaa aaacggatgc agccggcacg ttgggcggtc gggaaatacg
      241 ccttttcttc ggcactcaag gggcgcacgc cctcataacc gccgataaac acctttttca
      301 acgcctcatc caacttattg tccgccgtcc ttgcccaatc gttgaccgca atcgccaagt
      361 catacataaa attgccccgg caggcataat agaaatcgat gaaacccgat acctgaccgc
      421 cgtcaagcaa cacattgtct ttaaacaaat cggcatggat gatgcccgaa ggcagatgat
      481 tgccgagatt gtcctttaac gcatcgattt cggaacacag cagcgcggca tcgtcttgcg
      541 acaggacggg cagcagccgg gcgcacgcct ccgtccacca cgcattgtaa cgcgggtttt
      601 ccatttccaa agggaaatcg gcggcggcaa ggtgcatttt cgccaacatc gcgccggtat
      661 ggaaacactg ctcagccgtc ggcagcgcgg tatccgaacc tttcaggcag gcaaccaaac
      721 aagccggctt gcccgccaaa acggaatcaa gccggccgtc tttgcgcgca accggcgcgg
      781 caaccgccac gcccttcata ctcaaatgcc ggttaagctc cagaaaaaac ggcagctctt
      841 cctgtttcaa cacttcaaac acggtcagca cataacgtcc cgaagtcgtc gtcagaaaat
      901 aattgctgtt ggtaatcccc tgcgcgatgc cctgcaggga aacaaattca cccaaatcgt
      961 aaccgctcag gaagccgcgc atttcatcat cggaaacact ggtatagaca gacatatcaa
     1021 ctcacttgct caaaaacggg caaacccgcc gtcagaacgc cggacggcag gcaaacatat
     1081 aattcacatc ggtcgaatcg cacagggcat aagtttgcga caacacatgg taagtcatac
     1141 ccttcgtatc cgccacatcc aagcctgcct gacggcacat ccgcgccagc tcggcaggcg
     1201 cgatgaattt tttccagtcg tgcgtgcctt tggggacaaa cttcaacaga tattccgccg
     1261 ccacaatcag atgcaggtac gatttcgggt ttttattgat ggtggaaaaa aacaccatgc
     1321 cgtccggttt gaccagattg gcacaagcac gcacgatggc ggcgggatcg gggacgtgtt
     1381 ccatcatttc catgcacgtt accacatcga acgagtgcgg ttccgcctcg gcaaggtctt
     1441 ccacgcggat acattcgtat tcgatatcgg cgacattgtt caaagccgcg tgcaggcggg
     1501 cggtttccag cgactgctcc gccatgtcga taccctttac aaacgccgcg ccgcgccgtg
     1561 ccatactttc cgccaagatg ccgccgccgc agcccacgtc caaaacccgt ttgccgcaca
     1621 aatccgcgtg tccgtcgata taatccagcc gcagcggatt gatgtcgtgc aaggttttga
     1681 actcgcccga cttgtcccac catttgtcgg caatccggct gaatttggcg atttccccct
     1741 catcgacatt atattttttg tcggacattt tccctcccat ctgacgaacc gcccactcca
     1801 aaacccaaga ttatactatg gaacacaatg ccgtctgaaa gccttttcgg gcgacgaggt
     1861 gcaaatacct tacaaatcct tacactttac ggcataatgg cggcacgctt tttttggcag
     1921 aaagacaaaa tatgcccaac aaaacccctt ccctgttcgg cggcgcgatg attatcgccg
     1981 gtacggttat cggcgcaggc atgcttgcca acccgaccgc cacatccggc gtatggtttg
     2041 ccggctcgct gattgtattg ctctacactt ggttttctat gctctccagc ggtctgatga
     2101 ttttggaagt caacacccac tacccccacg gcgcaagttt cgacacgatg gtcaaagacc
     2161 tgctcggacg cggctggaac atcatcaacg gcatcgccgt cgccttcgtt ttatacctgc
     2221 tcacctacgc ctacatcttc gtcggcggcg acctgaccgc caaaggctta ggcagcgcgg
     2281 caggcggcaa tgtttcactc accgtcggac aactcgtctt cttcggcatt ctcgcctttt
     2341 gcgtatgggc atccgcacgc ttggtcgacc gattcaccag cgtcctcatc ggcggcatgg
     2401 tattaacctt tatttgggca accggcggcc tgattgccga tgccaaactg cccgtcctct
     2461 tcgacaccca agcccctacc ggcaccaact actggattta tgtcgccacc gccctgcccg
     2521 tctgccttgc gtcattcggt ttccacggca acgtctccag cctgctcaaa tactttaaag
     2581 gcgacgcgcc caaagtggct aaatccatct ggacgggcac actgattgcg ctggtaattt
     2641 acgtcctctg gcaaaccgcc atccaaggca acctgccgcg caacgagttc gcccccgtga
     2701 ttgccgccga agggcaagtc tccgtcctga ttgaaaccct gtccaaattc gcccaaaccg
     2761 gcaatatgga caaaatattg tccctgtttt cctatatggc gatcgccacc tcgtttttag
     2821 gcgtaacgct cggactcttc gactacatcg ccgacatctt caaatggaac gacagcgtgt
     2881 ccggccgcac caaaaccgcc gcgctgacct tcctgccgcc tctaatttcc tgcctgctct
     2941 tccccaccgg ctttgttacc gccatcggct acgtcggcct ggcggcaacc gtctggacag
     3001 gcatcatccc cgccatgctg ctctaccgtt cgcgcaaaaa attcggcgca ggcaaaacct
     3061 ataaagttta cggcggcttg tggctgatgg tttgggtctt ccttttcggc atcgtcaaca
     3121 tcgccgcaca ggtattgagc caaatggaac tcgtccccgt atttaaagga taaaggcaaa
     3181 atgccgtctg aagcccgccg gcggcttcag acggcattgc ctcgttatca cgcggcacag
     3241 gcgcatcact gccgatattt cagtataatg ccacccccga cctgccccaa tccaaaggaa
     3301 acgcgatgaa actcatcatt ctcgaccgcg acggcgtcat caatcaggac cgcgatgact
     3361 tcgtcaaatc cgttgacgag tggatacctg tcgaaggcag catggatgcg gtggcatttc
     3421 tgacgcaggc aggctacacc gtcgccgttg ccaccaacca atccggcatc gggcgcaaat
     3481 attttaccgt tcaaaacctt accgaaatgc acgccaaaat gcaccgcctc gtccgtcagg
     3541 caggcggcga aatcaacggc atctggttct gcccgcacac cgatgccgac aactgcaact
     3601 gccgcaagcc caaaccgggt atgattgaag acatcatcgg acgcttcaac gcccaagcct
     3661 cggaaacctg gctggtcggc gacagcctgc gcgatttgca ggcaatcgat gccgtcggcg
     3721 gaaaacccgc gctggttctg accggaaaag gcaaaaaaac gctctcccaa cacggacacg
     3781 aattacccga acacacacag gttttcgata ccttgctcga tttctcacaa tacatcatgc
     3841 aggaaaacgc cgcaccgcaa gccgactgaa cataccgcat tccgacaagg caaaaccatg
     3901 ctcatcatcc gcaacctgat ttactggctg atactctgtt ccaccctgat tttcctcttt
     3961 ccctttatgc tgctcgcctc gcctttccga gacggggcgc acaagatggc gcgggtctgg
     4021 gtcaaaatcc tcaacctctc gctcaaacac atcgtcgggc tcaaataccg catcatcggc
     4081 gcggaaaaca tccccgaccg ccccgccgtc atctgcgcca aacaccaaag cggctgggaa
     4141 acgctcgccc ttcaggacat ttttccgccg caggtttacg ttgccaaacg cgagttgttc
     4201 aaaatcccct ttttcggctg gggcttgaaa ctggtcaaaa ccataggcat agaccgcaac
     4261 aaccgccgcg aagccaacga gcagctcata aaacaggggt tggcgcgcaa aaacgaaggc
     4321 tattggatta ccattttccc cgaaggcaca cgccttgcgc ccggaaaacg cggcaaatac
     4381 aaactcggcg gcgcgcgcat ggcgaaaatg tttgagatgg acatcgtccc cgtcgccctc
     4441 aacagcggcg aattttggcc gaaaaactcc tttctgaaat atccggggga aatcaccgtc
     4501 gtcatctgtc cgaccatccc gcacgcaagc ggcagcgaag ccgaattgat gggaaaatgc
     4561 gaacacctca tcgaaacgca gcagccgctc atttccggcg caggcccgtt tgccgccaaa
     4621 atgccgtctg aaaccgcatg accgcctttg tccacaccct ttcagacggc atggaactga
     4681 ccgtcgaaat caagcgccgt gccaagaaaa acctgattat ccgccccgcc ggcacacata
     4741 ccgtccgcat cagcgtccca ccctgcttct ccgtctccgc tctaaaccgc tggctgtatg
     4801 aaaacgaagc cgtcctgcgg caaacactgg cgaaaacacc gccgccgcaa actgccgaaa
     4861 accggctgcc cgaatccatc ctcttccacg gcagacagct tgccctcacc gcccatcaag
     4921 acacgcaaat cctgctgatg ccgtctgaaa tccgtgttcc cgaaggcgca cccgaaaaac
     4981 agcttgcgct gctgcgggac tttttggaac ggcaggcgca cagttacctg attccccgcc
     5041 tcgaacgcca cgcccgcacc acacaactgt tccccgcccc ctcctcgctg acctctgcca
     5101 aaaccttctg gggcgtgtgc cgcaaaacca caggcatacg cttcaactgg cggctggtcg
     5161 gcgcaccgga atacgttgcc gactatgtct gcatacacga actctgccac ctcgcccacg
     5221 ccgaccacag ccgcgcgttc tgggcactga cccgccgctt cgcaccctac acgcccgaag
     5281 cgaaacagtg gctcaaaatc cacggcaggg aacttttcgc cttaggctga cgcggaccgg
     5341 accgacccgc cgctttcaga cggcatccgt gccggaacag gcacgcgccc gcccgattca
     5401 aaccgcgatg acgctttgcc gccggttcgg ggcaggatgg cggcacacac gccgtctgcc
     5461 gcgtttcatt tcacaccact cttccgaaac ccgaaacccg cccggtccga cgtgcggtat
     5521 gaaacgctta agctgacgca aagtctttta ctgatttgcc cgcgaaaatg ccgtctgaaa
     5581 ggttttcgga cggcattttt ttgcgtttcc caggatggcg gcggattcgt aaaaggcggt
     5641 cagggtggat tgtaggatgg gttgagacct gccgaatccg ccgcatctgc caaatctacc
     5701 gccgtcattc ctacgaaagt gggaatctag aacgcggggt taagaaaacc tgcaccgcgt
     5761 cattcccacg aacctacatc ccgtcattcc cacgaaagtg ggaatccaga acgtaaaatc
     5821 tgaagaaacc gttttacccg ataagtttcc gcaccgacag acctagattc ccgcctgcgc
     5881 gggaatgacg ggatttgagg tttctg
//
LOCUS       DAWHRS010000130         5889 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWHRS010000130 DAWHRS010000000
VERSION     DAWHRS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.130"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            132..566
                     /locus_tag="WIB82_001720"
     CDS             132..566
                     /locus_tag="WIB82_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ3842992.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(579..1442)
                     /locus_tag="WIB82_001721"
     CDS             complement(579..1442)
                     /locus_tag="WIB82_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ3842993.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYANKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1450..2067)
                     /locus_tag="WIB82_001722"
     CDS             complement(1450..2067)
                     /locus_tag="WIB82_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ3842994.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKSNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2230..2844)
                     /locus_tag="WIB82_001723"
     CDS             complement(2230..2844)
                     /locus_tag="WIB82_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ3842995.1"
                     /translation="MKTSNFSKRTSAMKTFALILAGGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQTDLQEG"
     gene            3050..4822
                     /locus_tag="WIB82_001724"
     CDS             3050..4822
                     /locus_tag="WIB82_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ3842996.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQPQQGTTVSLTGASEESLK"
     gene            4819..5475
                     /locus_tag="WIB82_001725"
     CDS             4819..5475
                     /locus_tag="WIB82_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ3842997.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1445 a         1661 c         1366 g         1417 t
ORIGIN      
        1 gttccgattc cgccgttatg tctgcctgcc ggacatacgt acgcattatt aacaaaagtt
       61 aaccgcgata ataccatctt tcacacgtca atctagtata tttcctaaaa tttccaacaa
      121 gaggaaaagc cgtgccactg cctgctccct gccgttttgc caaacctgcc gcctcttttt
      181 taagtatggc tttgctttcc tgccagcttt cccacgccgc cacggcttat atccccctga
      241 acgattttca accgaactgc gacatacgcc ggctcggact gacacagggt cagcacaatg
      301 aactgcgtaa aatccgcgcc gccttcaaaa tggcgggcga cagggcgcgt ttgaaggtta
      361 tgcattccga acacagccgc cgccggtctg tcgtcgaaat catttcctcg gatgttttta
      421 atcggaacga ggcgcgcgat tatgtcgaaa gccgctattt gtccggtatg gattttgcgg
      481 tggacgaatt ggaaatccaa caccggttct tccatatcct cacaccgcaa cagcagcaaa
      541 tgtggctttc ttcctgcctc aaataatccc cgaaacgctc acaacgcccg ttgtttcggc
      601 agcctgcccg cccagtcgca ggcaaactgc cacgcggaac gtcccgaacg gctgccccgc
      661 gtctgcgccc actgcaatgc cgccatctgt gcggtttcat catacggcac gccgaaatct
      721 tccagccaat tttgcaccgc cgccagataa tcgttttgat cgaacggata aaaactgagc
      781 cacaatccga atcggtcgga cagggatatt ttttcttcca ccgcttcttt ctgatggatt
      841 tccccccgca tccccgtcgt accggcattc tcgtcaaaat attcgggcat cagatgccgt
      901 ctgttggaag tcgcgtaaac caaaacgttg gcgcaacgtt gagacagacc gccgtctaac
      961 gcggttttca atgccttata ggtttcatcg ccgctttcaa acgacaaatc gtcgcaaaat
     1021 acgataaatt tttccggacg ttccttcaaa agcgtcaaca ggtaaggcag gccgattaaa
     1081 tcgcttttat cgacttcgat caggcgcaat cccttattcg catattcgtg tagcagggct
     1141 ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca tcaatacgtt attcgcgggt
     1201 ctgccgacaa tgaactgttc ggtattacgc accagcaatt cggtttgcct gccgactccc
     1261 gccagcctta ccaagggaaa ggtgtgcgga tcgggcaagt gttccaaaaa accttttttg
     1321 cccgcactct gccagcggaa ggcaagcgcg ttccaatccg tatgcccggg ttcgggcgga
     1381 agcacggcat ccaaacgccg caaaacggca taggctttat cgagaaattc gttcaattcc
     1441 atctctacct cactttgcat atctttgcgc catcagccgt tcgacggtgt cgacgattgc
     1501 ctgcgtattc ggatcgattt cgatgttgat cctgtcgccg acctttctgc tgccgaacag
     1561 cgtccgttcc aaagtttcgg gaatcagatg gacattgaaa cggccgtctt cgactttgcc
     1621 tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg gtcaggatat agggtttgag
     1681 ttcatgcggg agcgaaaacc aaaccgtgcg gttgaacccg tcccgttcga tttcgacaat
     1741 aggcacggtt gccataatgt gtccgctcat gacgtgtccg ccgatttcgt ctccgaaacg
     1801 cgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc agccccaaat tgctttttgc
     1861 caaagtttcc gccattaaat cgaagctgac gcggtttcct tcgatttcgg taatcgtcag
     1921 gcagcagccg ttattggcaa ccgatgcgcc gcgttgcaga ttgtccgccg cctcttgcgg
     1981 aagctcgacg acataagtct gaaatgcctc cgacgggcgg tggattgccg tcagttttcc
     2041 caatccttga acaatgcctg taaacataat cctgtttccc tgtgtcggta aaaatggtgc
     2101 aaattgtagc atttccccgc gaaaaatgcc gtctgaaatg ccttcagaca gcattatgcc
     2161 tccgattcgg gcaaaaaccg cccggtatgg cttgaccttt cctttccacg ccggtcggcg
     2221 gtcttgccct tatccctcct gcaaatcggt ttgcgtgttc aagtcggcaa aatgcccgtc
     2281 aaactcgaat ctgaccgacc gcgccctttg ctgctgcaac cagcttctta atgttttcat
     2341 acccgaaaac agatagggaa tcgcgctttg cagaatctgc gggcggatat acataatgtt
     2401 gtagtgcatc gttatcggcg tttccacata atacgcattg cacaacggtg tgcgtttcga
     2461 cacggtttca aacctcgcca ccaaatcgtc cggcagatac ggcatatcgc acggcacaac
     2521 caaaagccag tcagcagtcg ccaactgcaa atcgttggct gcggtacaca atgccgaaag
     2581 cgggccgaaa tgctgccact gccgcgcatc gggaaaaata tgcggacttc ttcgagcata
     2641 ttcttccaaa ttccggttgg tgctgatggc gatatggctg acctgcggcc tgaccctgtc
     2701 gatgacatgg tctatcagtg ccttaccccc caaaagagca agccccttgt cctcgcctcc
     2761 catacggctc gcctgaccgc cggccagtat cagggcaaaa gttttcattg cggatgttct
     2821 cttggaaaag ttcgaggttt tcatgattgc agtctgccgt tcccaatgac tcaaaatgcc
     2881 gtctgaagca gacggcaaat aaattcatat tatctgaatt ttatcataac atgatttaac
     2941 actgaagcgg tgcggattaa gttttcctta accaattctt tctgagcagt tgtatctaat
     3001 tccaaagaat gatattgttt gcattattta gaacaatttt tcgccgagca tgatactgcc
     3061 agcccgtttt tcagacggca tcagcctttc cctgcgcctg aaactcctga ccggactgtg
     3121 ggtcgggttg gcggcattgt ctgtcgtttt gacactgctg ctctctttgc gtctggaaaa
     3181 cgcggcctcc gtcatcgaag aggcgggcaa cttgagaatg caggcatacc gtctggcata
     3241 catggcgggt gaaggctcgc cccgtgcgca aattgacaat caggttgccg aatttgaaaa
     3301 aagtttaaaa cgcattgccc aaagcgatgc catccatccg ctgattcctt cggacacccc
     3361 tcttgcttat gatttgatac aatccatgct gattatagat tggcaggcac acatcctccc
     3421 cccgctccag tcctaccggc gaccgactca ggtcgatctc taccgctttg ccggaaacat
     3481 cgaactgttt ttgcaggcat tggaaaatgc caacgaaaaa aacacatggt ggctcaggcg
     3541 ttttcaatgg gcaattatgt tgatgacgct ggtgtcgtct gtactgatgc tgttttggca
     3601 ccagatttgg gttatccggc cgctgcaggc gttaagggaa ggtgcggaac gcatcggacg
     3661 gaggtgtttc gatattccgg ttcccgaagg cggtacgccg gaattcaaac aggtcgggcg
     3721 ttgtttcaat caaatgggcg gcaggttgaa aattttatat gatgatttgg aaggacaagt
     3781 cgccgagcag acacgcagtc tcgaaaaaca aaatcaaaac ctgaccctgc tgtaccaaac
     3841 tacacgggac ctgcaccaat cctacatacc gcaacaggct gcagaacatt ttctaaaccg
     3901 tatcctgccc gccgtaggag cagattccgg cagagtttgt ttggacggcg gatccgatgt
     3961 ttatgtttcc attcatcatg cggattgcgg cacagcagct tcggatttgg ggaagtacca
     4021 tgaggaaatc ttccccattg agtaccagaa cgaaacattg ggcaggctgt tgctcagctt
     4081 tccaaacggc atttctcttg atgaagacga ccgcatcctg cttcaaacac taggcaggca
     4141 attgggcgta tcgcttgccg gcgcaaaaca ggaggaagaa aaacgcctgc ttgcagtatt
     4201 gcaggaacgc aacctgattg cgcaaggatt acatgacagc atcgcacaag cattaacgtt
     4261 cctaaaccta caggtacaga tgctggaaac cgcctttgcc gaaaacaaac gggaggaagc
     4321 cgcagaaaac atcggcttca tcaaaacagg cgtgcaggaa tgttatgaag atgtccgcga
     4381 actgctgctc aacttccgta ccaaaatcag caataaagaa tttcccgaag ccgttgccga
     4441 cctattcgcc cgctttacgc aacaaaccgg gataacggtc gaaaccgcct gggaaaacgg
     4501 ttcgttcctg ccgcctcagg aagcgcagct ccaaatgatt tttatcctgc aggaaagcct
     4561 gtccaacatc cgcaaacacg cccgcgccac ccatgtaaaa ttcacccttt ccgaacacgg
     4621 cggacgcttt accatgacca tccaagacaa cggacaaggt ttcgacacgg agaaaatagg
     4681 agaacctacg ggcagccatg tcggactgca catcatgcag gagcgtgcca agcgcatcca
     4741 tgccgtttta gaaatccgtt cccaacccca acagggaacc accgtctcat tgacgggtgc
     4801 atctgaagaa agcttgaaat gaccattaaa attattctga tagacgacca caccctcttc
     4861 cgcagcggca ttaaagccct tttgtcgcgc caacacggtt ttgaagtcat cggcgaagcc
     4921 gcagacggcc tctcgggcat caaaatgatt agtcggctgc aacccgatgt cgtcctgctc
     4981 gatttggata tgcccggcat gaacggacgc gaagcactct cccaaatcat cagcatcaat
     5041 ccgcagcagg cagtgattat gctgaccgtt tccgaagaca gcgacgactt gaccgaatgt
     5101 attcgcatcg gcgcgcgcgg ttacctgctg aaaaacatca acgctgactt tctgctcgaa
     5161 agcatacgca aagccgccga aggcgataat gtattctcgc ccgagatgac cgccaaactc
     5221 gttaaaagcc tgatttcccc ccaacctgcc caagggacac aggcactctc ctcacttacc
     5281 cctcgcgaac tggaaatctt gggctatctc gccgcagggc acagcaacaa aatcatcgcc
     5341 cgccacctcg atcttgccga atccaccgtc aaagtccacg ttcaaaacct gctccgcaaa
     5401 ctcaacctca gcagccgggt gcaggccgcc gtttacgcca tccggcacaa cgtcccccaa
     5461 cccgtgctgg aataaggcgg gttcagacgg catattaggg gttttaatcc ccgtacggtc
     5521 attcggataa cagaccacgc atgtaagctt atgcccccat aagtacgctt ggcatagcag
     5581 taatattgtt cggtttagtg ttttccgttt gcccctatct gatactgcaa tatcagctat
     5641 gccgtctgaa aacgcatcat catgatattt tcagacggca taataaaaag cggaaatact
     5701 aatgcagggt aaaatgttcc attccgaatc ccataaatat acaatggctt atatcgttta
     5761 agcatgtgta acccaccctc atatcaatca atatatagtg gattaacaaa aaccagtacg
     5821 gcgttgcctc gccttgccgt actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     5881 ttttgttaa
//
LOCUS       DAWHRS010000131         5869 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWHRS010000131 DAWHRS010000000
VERSION     DAWHRS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.131"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..761
                     /locus_tag="WIB82_001726"
     CDS             <1..761
                     /locus_tag="WIB82_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ3842998.1"
                     /translation="AVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDF
                     RAWNGMTDNMLSIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPVQSAP
                     QPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAG
                     NAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQ
                     QVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
     gene            complement(1122..1604)
                     /locus_tag="WIB82_001727"
     CDS             complement(1122..1604)
                     /locus_tag="WIB82_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ3842999.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(1757..1918)
                     /locus_tag="WIB82_001728"
     CDS             complement(1757..1918)
                     /locus_tag="WIB82_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843000.1"
                     /translation="MSGQPEKHHTSPIEDKRKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(1992..2453)
                     /gene="recX"
                     /locus_tag="WIB82_001729"
     CDS             complement(1992..2453)
                     /gene="recX"
                     /locus_tag="WIB82_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ3843001.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(2526..3236)
                     /locus_tag="WIB82_001730"
     CDS             complement(2526..3236)
                     /locus_tag="WIB82_001730"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ3843002.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            3643..4911
                     /locus_tag="WIB82_001731"
     CDS             3643..4911
                     /locus_tag="WIB82_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ3843003.1"
                     /translation="MSSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRV
                     LEVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCC
                     VAGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIM
                     SWMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGA
                     TYAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITN
                     EAGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGSTTPEADVI
                     LRQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNL
                     AQEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(4969..5775)
                     /locus_tag="WIB82_001732"
     CDS             complement(4969..5775)
                     /locus_tag="WIB82_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ3843004.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1279 a         1377 c         1714 g         1499 t
ORIGIN      
        1 ccgccgtacc ggctgccgcg caaccgcccg tacagtccgc acccgtcgac atcaacgcgg
       61 cgacgcatac tattgtgcgc ggcgacacgg tgtacaacat ttccaaacgc taccatatct
      121 ctcaagacga tttccgtgcg tggaacggca tgaccgacaa tatgttgagc atcggtcaga
      181 ttgttaaagt caaaccggca ggatatgccg caccgaaaac ctcagccgta gaaagcaggc
      241 ccgccgtacc ggctgccgtg caaacccctg tgaaacccgc cgcgcaaccg cctgtgcagt
      301 ccgcgccgca acctgccgcg cccgctgcgg aaaataaagc ggttcccgcg cccgccccgc
      361 aatctcctgc cgcttcgcct tccggcacgc gttcggtcgg cggcattgtt tggcagcgtc
      421 cgacgcaagg taaagtggtt gccgatttcg gcggcaacaa caagggtgtc gatattgccg
      481 gtaatgcggg acagcccgtt ttggcggcgg ctgacggcaa agtggtttat gcaggttccg
      541 gtttgagggg atacggcaat ctggtcatca tccagcataa ttcttccttc ctgaccgcat
      601 acgggcacaa ccaaaaattg ctggtcggcg aaggccagca ggtcaaacgc gggcagcagg
      661 tcgctttgat gggcaatacc gacgcttcca gaacgcagct tcatttcgag gtgcggcaaa
      721 acggcaaacc ggttaatccg aacagctata tcgcgttctg actttctgcc tgaaatcaag
      781 ttggaaatgc cgtctgaaag gttttcagac ggcatttttg tttttggcgg ctgtgttttc
      841 aaatcattcg gaagggatgg ggcatataaa tttgtttgac tgtgtttggg tatagtggat
      901 taacaaaaat caggacaagg cgacgaagcc gcagacagta cagatagtac ggaaccgatt
      961 cacttggtgc ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgccg
     1021 tactggttta aatttaatcc actatatttg cttgatttca tccgtttgca atggaaacgg
     1081 atggcgcgat gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggacatg
     1141 tcttcagagg cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt
     1201 tggcggtcgg tcaggatttc cagcggaggg acagggacgg gtttgccgtc tttgaccgca
     1261 accatcgtga agtagcagct gttggtatgg cggatttcgc cggtgcggat gttttgtgct
     1321 tcgacacgga tgccgatttc catcgaggtg cgtcccgtgt agttgacagc ggcgtaaaag
     1381 gtaaccaaat cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgacagggta
     1441 acgcaataat tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc
     1501 aggagttcgc cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg
     1561 gacataatga gttcgtgcga aggcagttgg cgttgttgtg tcatggtgta cctgttttaa
     1621 gaaagggtgt tttgaaatgg gtgcggggaa attttaggga atggggaagt ggaattcaag
     1681 ctaaaaaaag ctatggcggg atagtctgca gaaatgggcc gccgatgccg tctgaagcct
     1741 tcagacggca ttttgtttag gaaagccaaa aacggaagac caggaaaaac acgagtgcca
     1801 atacggcgac ccaaacgacg agcatgaatc cggcaactgc ccgtcccata cggtaaaccg
     1861 ggtttttgcg tttgtcttcg atgggggagg tgtggtgttt ttcgggttgt ccggacattt
     1921 tgttctcaat cggaaaagtt ggaaacgggc gtaaggttat gttgccgtgc aaaaagattc
     1981 aagaaggaag gtcagcagcc gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc
     2041 tgaacggtat cggcatcgaa tccgcgatag gcgaggaaac gcgcctgttt ttgtttttct
     2101 ttgaggtcgg cggcggggtg tttgaatttt ttacgcaaca cggctatggc ggcctgtttt
     2161 tcgcttgagc ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc
     2221 aaagcctgtt tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg
     2281 gcatagcgca aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac
     2341 tcctcttcgc tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgacttct
     2401 tggcgcgaga ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg
     2461 gtttcggcac ggaaagattc agacggcatg ggaaacggat tccgatgccg tctgaacaga
     2521 ttgttttatt cttcgtcttt atttttctgc ggttgcaggt tttcgtaaat ttcgggcagc
     2581 gagccgataa tccagtcggg gcgggtcgca tcgtcttgcg agagcagcgt catatcgccg
     2641 taaccgaagg taacgccgac gctcaggcag ccggcggctt tggcggcgat gatgtcgttg
     2701 cgcgagtcgc cgaccataac catgtttgca acatcgatac ccaaaacttc ggcggcgtgc
     2761 cgcagcggca gggggctggg ttttttctcg ggcaggctgt cgccgccgag tatcaggctg
     2821 aagtagtcgg caagtccgag ctgtttcaac agttcgacgg caaggatttc gtttttattg
     2881 gtaatgactg ccagcgggat gcccaaagat ttgagcaatg ccagcccggt tgcggtttcg
     2941 ggataggggc gggtgaagac gctcaaatgg tcgcggtagt atttcatata ggatacgaaa
     3001 cctttttccc acagttcgga atcggcttcg cggtcgcggt cgttggagag gacgcggtga
     3061 accagtttgc cgatgccgtc gcccacatag ctttcgacca ctttggcggg cagcggtttc
     3121 ataccgagtt gttccaacat cgcttctgcg gcggcggcaa ggtcggggac ggaatcgcac
     3181 agtgtgccgt ccaaatcgaa ggcgacggct tggacgtgtt cgatggctgc attcataatc
     3241 tgatttcctg tcgggttgaa agggtttatt ttaacatcgt ttgcacaggg cttctaatgt
     3301 atagattgtt aacaatcgca acaggttttg caggcggtgt tttcggattt cggggcggaa
     3361 tcgggcaagg gtttgaaatc ccagcgatgt tccaattaaa tttttaaaaa ataaaaggat
     3421 gcctatatct ttatttaatt tgacatttat ttttcggacg cgcgcgctgc tttttcaggc
     3481 ggagtccggg ctgattccgt gcggcttgaa aaataaaatc atatatagat taatcaaatc
     3541 gggcttatgc cgactacacg ctattacatt ttgtcgggaa attggttata gtaatgagcg
     3601 aaatgcacgc ccctgaaaat ctttttgtga aaaggaagca aaatgtcgtc tgaagccctt
     3661 gcaaaagaaa ccctcaatcc gtttgaaatc gcgcgcaaac aggtcaaaac cgcctgcgac
     3721 cggctgaaaa ccgatccggc cgtttatgaa atcctgaaga gtccgacccg cgtgttggaa
     3781 gtcaatttcc ccgtcaaact cgacgacggc acggtcaaaa ccttcacggg ctaccgttcg
     3841 caacacaaca acgccgtcgg cccctataaa ggcggcgtgc gcttccatcc cggagtgaat
     3901 ctggacgaag tcaaagccct gtcgatttgg atgaccatca aatgctgcgt tgcgggtatt
     3961 ccttacggcg gcggcaaagg cggtattacc ttggatccgc gcgattattc cgaggcggaa
     4021 ctggaacgca tcgctcgcgc ttattccgaa gccatttctc cgctgattgg cgagaaaatc
     4081 gatattcccg cccccgatgt gaataccaac ggcaaaatca tgtcttggat ggtggatgct
     4141 tatgaaaacg tggtgaaaca ttccgcgccg ggcgtattta ccggaaaacc ggttgagttc
     4201 ggcggctctt tggcgcgtac cgaggctacc ggttacggcg tgaacctcgc cgccgtccaa
     4261 gctttggaaa aactgggcaa ggacgtgaag ggtgccactt acgccattca aggtttcggt
     4321 aatgtgggtt accacacagg ttattacgcg catcaatccg gcgcgaaagt tgttgcggtt
     4381 tctactgtcg atgttgccat ttacaacgaa aacggcttgg atatggaagc tttgtttaaa
     4441 gagttccaag aaaaaggctt catcaccaac gaagccggtt acggcaaaga aattaccaac
     4501 gccgaacttt tggctttgga tgtggacgta ctcgcccctt gcgcgctgga aaaccaactg
     4561 acttccgaaa acgccggtaa agtccgcgcg aaaatcgtgg tcgaaggcgc gaacggctcg
     4621 actacgcccg aagccgacgt tatcctgcgt caaaacggcg tattggtcgt gcctgatatt
     4681 ttggcgaact gcggcggcgt ggtggtatct tattttgaat gggtacaaaa cctgcaaggc
     4741 tattattggg agtttgacga agttcaagag aaagaaaccg tcgtcctgcg acgcgcgttc
     4801 cgcgatatat ggaacttggc tcaagaatac gatgtcgatt tgcgaaccgc gtcttacatg
     4861 atgagtatcc gccgtgttga aaaagcgatg aagctgcgcg gttggtattg atgtttgaac
     4921 aaaaaatgcc gtctgaagcc ttcagacggc atttttgtgc gggcgggatt aatggtgcga
     4981 atggccgatt tggaaggtta aagtcgagta gttcgcctgt ttttggcaca cgctttgatc
     5041 ggggaagtcg gctttgtgtt cgacattggc tttccagaag ccttggcgca aggggatgat
     5101 gtccacttcg cctttgtcgt ctgtgctgtc ggagaaagcc tgtgcttcgg ttttgtgcgt
     5161 tttgctgcgg tcgctggtgt cgaagccgtc aaaggtggcg gtaacggtgg cattgggcag
     5221 cggttcgcca cggaacagaa cgcggacttt gaagcgttcg cctacgtgaa tgttggcggg
     5281 attgtccagc gggacgattt ccaagttttg tccgaccggt ttggtgatga tggcggtgtc
     5341 cgcgctttcg tgtccgacgt tgacgatgtt tttgccgaac attcgggttt gttcgcaata
     5401 gcttgcgtca ggcatttctt tgatgcccgc ctgtttccag cctgctttgt tttttgacca
     5461 gaaagtaggc tgatattcgg cgatgacgag gtaactgccg tccttaacgg gacggttgct
     5521 tcggtactgg tagttgtatg tgccgcgttg aatcatgttt tccttgcctt tttcggtaac
     5581 cagctgcatc ggtttgctga agatgtgcag gcggtctttg gcgatgggtt cgagttcggg
     5641 aaattcgccg tagcccaagt cggctttaag gtattcgccg ccgtgcgtgt gggcggtttc
     5701 gacccagacg cggtgggcgt gggcggatgt ggcaaatagg gcggaaacgg cgagcagtgt
     5761 caatgttttc ttcatggtgc agcctttgtc tcgtgtgggt taaagatttt gttatgttat
     5821 aacaaaatag gtatcgtgtg aagatggatt tgctgtctga aaactgtct
//
LOCUS       DAWHRS010000132         5860 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWHRS010000132 DAWHRS010000000
VERSION     DAWHRS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.132"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            304..1737
                     /locus_tag="WIB82_001733"
     CDS             304..1737
                     /locus_tag="WIB82_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ3843005.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFLIKENKEALARLMTMEQGKGLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEVEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1847..2635
                     /locus_tag="WIB82_001734"
     CDS             1847..2635
                     /locus_tag="WIB82_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ3843006.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3003..4559
                     /locus_tag="WIB82_001735"
     CDS             3003..4559
                     /locus_tag="WIB82_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ3843007.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4576..5322
                     /gene="surE"
                     /locus_tag="WIB82_001736"
     CDS             4576..5322
                     /gene="surE"
                     /locus_tag="WIB82_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ3843008.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
     gene            5578..>5860
                     /locus_tag="WIB82_001737"
     CDS             5578..>5860
                     /locus_tag="WIB82_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ3843009.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPS"
BASE COUNT         1352 a         1506 c         1651 g         1351 t
ORIGIN      
        1 gttcggattt ttgccatact ctaccacacg ttgcaactgc aatctttgct ccttatatat
       61 tgcaccccat caaagggctg cattactttt ctttctaaag attactatcg tataaaaaat
      121 atttttctta aacgacaggg attgcgcccg cgccatattc agacaccgcc cggatgttgc
      181 gctgcccgat cggatgcttc agacggcatc ggaagggttt gcagttttgg aatatgaaga
      241 tgataatgtc cgcgagattg acggcatttg aaagcggtta ttccgcatac cggaggatac
      301 gaaatgaacg aatattccca attaatcaag catcccgata tttcccttcc cccggtttca
      361 gacggcatca aagtcgataa tccggcaacg ggcgagactt tggcgtttgt ccgcaagacg
      421 gattcggaca agctgaaaaa cctgattcaa aaggcggcgg cagcgcaaaa attatgggcg
      481 gcaaaaaccg cgttggaacg cgccgatgtg ttgtggcgtt ggtatttttt gataaaggaa
      541 aacaaagaag cactggcgcg tctcatgacg atggagcagg gcaaaggcct gaccgaggcg
      601 cgcggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgccgaaga ggcgcggcgg
      661 attgacggcg atgtgctgac gagtgtgaaa gcgtcgcaaa aactggtcgt gttgaaacag
      721 cctgtcggtg ttaccgccgc gattacgccg tggaactttc cgtccgcgat gattgcgcgc
      781 aaggctgcgc cagctttggc agtgggttgc gcgatgatag tcaaacccgc gtcgctcacg
      841 cctttgagcg cgtatgcgtt ggccgtgttg gcttacgaag cgggcgtgcc gcaggatttg
      901 ctgcctgttg tcagcggcag tgcttcggaa atcggccgtg aatttgccac aaatccgatt
      961 atccgcaaaa tcagtttcac cggctctacc gaagtcggcg cgaaaatttt tgccgacagc
     1021 gcggcagaca ttaaaaaact cagcttggaa ctgggcggca acgcgccgtt tatcgtgttt
     1081 gacgatgccg atttggacaa agccgtcgaa ggtgcgctcg ccagcaagtt ccgcaacagc
     1141 ggtcagacct gcgtctgcac caaccgcgtt tacgcccaat cgggcattta cgacgcattt
     1201 tgccgcaaat tgagtgaaaa agcagccgcg ctcaaattgg gcaacggctt ggaggatggc
     1261 gtcaaccaag ggccgctgat tgaggaaaaa gcggtagaaa aagtcgagca gcacatcgcc
     1321 gacgcgctct caaaaggcgc ggtctgtctg accggcggca aacgcagcgc attgggcgga
     1381 acgttttttg agccgaccgt cttaagcggc gtaacggcgc aaatggcggt ggctcgcgaa
     1441 gaaaccttcg ggcctttgtg tccggtattc cgttttgaaa ccgaagtcga ggtcatcgag
     1501 gctgcgaaca atacggaata cggtttggcg gcctaccttt tcaccgccga caccgcccgc
     1561 caatggcgcg tcggcgaagc cttggaatac ggtatggtcg gcatcaatac gggcttaatc
     1621 agcaatgaag cggcaccgtt cggcggcgtg aaacgcagcg gtttgggacg tgaaggcagc
     1681 aaatacggtg cggacgaata tctggaattg aaatatctgt gtatggatgt cgggtgacgg
     1741 atgccgtctg aacggcaggg tttcagacgg tgccgcattt taagcagtct ctatctgttg
     1801 tacaatgcgc cctgttttta cggttatttt tgatgtgaat taagatatga tggaaaacgg
     1861 aaaaacagtc ccgagatggc gttttgcctt gaaaagtgcg ggctggcacc tcttaatcag
     1921 cctgtcggtt gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta
     1981 tgccgagctg acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg
     2041 tggtccgctg ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt
     2101 cgatttttcc atggtcggca tcatccagct ggcggctttg gtgtacggtc tgcacagcgt
     2161 ttctttggcg cgtcccgtgg tggaggcgtt tgaaaaagac aggattaatg ttgtgaccgc
     2221 cgccgaagtg gcggttgaag acctgcacaa agcgccggaa gggctgcaaa gcctgtcgtg
     2281 gttcggcatc cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac
     2341 gctggatttg tccctgaaag gtatcgagcc gagtatgcgt cccgaccggt ggctgccgta
     2401 ttccgacaag gaagcagaag aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga
     2461 tgcgagaaaa acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga
     2521 gctgtattac ctgccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa
     2581 agagggcaac accaaaggct acgcgccgat agacggcttc atcatcaccc cttaagcgtt
     2641 gggactccgt ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga
     2701 gaggggcgca acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg
     2761 tgttgaaaca tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcat
     2821 tttgccattg ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc
     2881 acatgaaaga ccccgagcag agcagcaagc ccgcccgccg ttttttgtgc gtaccgtctg
     2941 ttcaggcagc ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa
     3001 tcatggattt cagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg
     3061 tgttggaagt cgtattgggg atagacaacc ttgtctttgt ggcgattttg gcaaacaagg
     3121 tccagcccgc acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc
     3181 gcatcattat gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa
     3241 tcggcggcct cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc
     3301 tttacaaagc caccaccgaa ctgcatgaac gcctcgaagg gcacaaccgt tttaccgttg
     3361 ccgacaacca aaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg
     3421 atgccgtgtt ttccatcgat tcggtcatta ctgcggtggc gatggtcgat catatcgtcg
     3481 tggcgatggg tgcggtcgtc gtcgcaatgg ctgtaatgat ttctgccagc aaactcttga
     3541 ccgaatttgt cgacagacac cctaccgtcg tgatgctctg ccttggtttt ttgttgatga
     3601 tcggtttcag cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg
     3661 ccgccatcgg cttctcgatt ttaatcgaac tgtttaacca gatttcccag cgcaacagcc
     3721 gcaaaaacga ctacatcggc agctcgtggc gcaagcgcac cgccgaaaac gttttgggta
     3781 tgatgggcat acgcgaaagc gtgcttgccg atgcgggcgg cgaatccggg gacgacgcgc
     3841 attttgaaga aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga
     3901 ttatgggggt gatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg
     3961 aagaacagtg tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggtaagg
     4021 cgggcgtgga cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg
     4081 aaacaggcgg tctcgacatt cagacggcat tgcgccagcc actcgtcctg cccgacagca
     4141 ccaccgcgct gggcgcaatc gaactcttcc gccaaagcag cgcggattat gctttggtgg
     4201 tggacgagtt cggcgcggta ttgggcatgg taaccatgaa agacctgctc gaaaccatcg
     4261 caggcgagtt ccccgaagaa tttgagcgcg aagaagaacc agccgttcag gggaatcccg
     4321 atgaaagcct gacggtggaa ggcgcgttgg aatatgtgga actcgcaccg caactcaacc
     4381 tgccgcagca ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat
     4441 tgcaaaccat ccccgatgtc ggcgattttg ccgatttcca cggctggcgg tttgaagtgg
     4501 tcgaaaaaga agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag
     4561 tattcaggat agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggc
     4621 atcgccgttt tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt cgcgcccgaa
     4681 cgcgacagga gcggggtcag caattcgctg acactggaac gccctttgca gttgaaacag
     4741 gcgcaaaacg gcttttacta tgtcaacggc acgccgaccg actgtatcca catcgggcag
     4801 tctgtatttt cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat
     4861 atgggggacg acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg
     4921 ggcatacccg ctgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcaaccgca
     4981 gaaaaggcac tgtggacatt gttggcgcat tttttcaaaa accccccgca gtcccctatt
     5041 ttgtggaaca tcaatatccc cgccgttgcg ccggaagatg tgcggggcat taaaatcgcc
     5101 cgtttgggca ggaggcatca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa
     5161 cagatttatt ggataggacc ggtcggcgaa gtttccgatc gggaagaggg aacggatttc
     5221 ggtgaatgcg gcgcaggttt cattaccgta acgccgctgc aaatcgacct gaccgcctat
     5281 ccggacatgg cggaaacagc ggcgttctgg catgcggact gaccgtttca tcaaatatag
     5341 tggattaaca aaaaccagta cggcgttgcc tcgccttagc tcaaagagaa cgattctcta
     5401 aggtgctgaa gcaccaagtg aatcggttcc gtactatttg tactgtctgc ggcttcgtcg
     5461 ccttgtcctg atttttgtta atccactata ccgtcgggct ttgcgcttga gaagaacgcg
     5521 catttttatt atgatatacc gcattcaggc tatatatcgg aacatgaagg ttatcttatg
     5581 ttgaaacaaa cgacactttt ggcggcttgc gcctcctttg ccgcactgtt gggcggttgt
     5641 gctacccagc agcctgcccc cgtcattgca ggcaattcag gtatgcagga cgcgccgtct
     5701 tcggcagttt acaacaaccc ctatggagca acgccgtaca gcccggctcc tgccggcgat
     5761 gcgccttatg tgccgccggt gcaaagcgcg ccggtttata cgcctcctgc ttatgttccg
     5821 ccgtctgcac ctgccgtttc gggtacatac gttccttctt
//
LOCUS       DAWHRS010000133         5789 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWHRS010000133 DAWHRS010000000
VERSION     DAWHRS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.133"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            405..1945
                     /locus_tag="WIB82_001738"
     rRNA            405..1945
                     /locus_tag="WIB82_001738"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2047..2123
                     /locus_tag="WIB82_001739"
     tRNA            2047..2123
                     /locus_tag="WIB82_001739"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2081..2083,aa:Ile,seq:gat)
     gene            2129..2204
                     /locus_tag="WIB82_001740"
     tRNA            2129..2204
                     /locus_tag="WIB82_001740"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2162..2164,aa:Ala,seq:tgc)
     gene            2610..5501
                     /locus_tag="WIB82_001741"
     rRNA            2610..5501
                     /locus_tag="WIB82_001741"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5598..5711
                     /gene="rrf"
                     /locus_tag="WIB82_001742"
     rRNA            5598..5711
                     /gene="rrf"
                     /locus_tag="WIB82_001742"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1619 a         1237 c         1689 g         1244 t
ORIGIN      
        1 caaaggcgca ccggtcagag gagggggagg aagggattgt tggaggcggc gcagcattta
       61 gcagaaataa aaaaccttat ccgacagcga catgacgaat ttccccaaaa aaatcccact
      121 gaaagcattg accgtttttc cctgtgggcg tatagttcgg ttcttcgctg ctgcagaagc
      181 ggcgggcgaa ctgaaaagta tagcacagaa tgttggggat atcgagagat atcttgacag
      241 gcggaagaaa tactttataa ttcaaacgct ctttaacaaa acagattacc gataagtgtg
      301 agtgccttga gcctcacact gtttgaaaga cagacaagat aatgttttga acattgtcct
      361 gttggtttct ttgaagcaga ccagaagtta aaaagttaga gattgaacat aagagtttga
      421 tcctggctca gattgaacgc tggcggcatg ctttacacat gcaagtcgga cggcagcaca
      481 gagaagcttg cttctcgggt ggcgagtggc gaacgggtga gtaacatatc ggaacgtacc
      541 gagtagtggg ggataactga tcgaaagatc agctaatacc gcatacgtct tgagagagaa
      601 agcaggggac ctttgggcct tgcgctattc gagcggccga tatctgatta gctggttggt
      661 ggggtaaagg cctaccaagg cgacgatcag tagcgggtct gagaggatga tccgccacac
      721 tgggactgag acacggccca gactcctacg ggaggcagca gtggggaatt ttggacaatg
      781 ggcgcaagcc tgatccagcc atgccgcgtg tctgaagaag gccttcgggt tgtaaaggac
      841 ttttgtcagg gaagaaaagg ctgttgctaa tatcagcggc tgatgacggt acctgaagaa
      901 taagcaccgg ctaactacgt gccagcagcc gcggtaatac gtagggtgcg agcgttaatc
      961 ggaattactg ggcgtaaagc gggcgcagac ggttacttaa gcaggatgtg aaatccccgg
     1021 gctcaacccg ggaactgcgt tctgaactgg gtgactcgag tgtgtcagag ggaggtagaa
     1081 ttccacgtgt agcagtgaaa tgcgtagaga tgtggaggaa taccgatggc gaaggcagcc
     1141 tcctgggata acactgacgt tcatgcccga aagcgtgggt agcaaacagg attagatacc
     1201 ctggtagtcc acgccctaaa cgatgtcaat tagctgttgg gcaacttgat tgcttagtag
     1261 cgtagctaac gcgtgaaatt gaccgcctgg ggagtacggt cgcaagatta aaactcaaag
     1321 gaattgacgg ggacccgcac aagcggtgga tgatgtggat taattcgatg caacgcgaag
     1381 aaccttacct ggtcttgaca tgtacggaat cctccggaga cggaggagtg ccttcgggag
     1441 ccgtaacaca ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt gggttaagtc
     1501 ccgcaacgag cgcaaccctt gtcattagtt gccatcattc agttgggcac tctaatgaga
     1561 ctgccggtga caagccggag gaaggtgggg atgacgtcaa gtcctcatgg cccttatgac
     1621 cagggcttca cacgtcatac aatggtcggt acagagggta gccaagccgc gaggcggagc
     1681 caatctcaca aaaccgatcg tagtccggat tgcactctgc aactcgagtg catgaagtcg
     1741 gaatcgctag taatcgcagg tcagcatact gcggtgaata cgttcccggg tcttgtacac
     1801 accgcccgtc acaccatggg agtgggggat accagaagta ggtaggataa ccgcaaggag
     1861 tccgcttacc acggtatgct tcatgactgg ggtgaagtcg taacaaggta gccgtagggg
     1921 aacctgcggc tggatcacct cctttctaga gaaagaagag gctttaggca ttcacactta
     1981 tcggtaaact gaaaaagatg cggaagaagc ttgagtgaag gcaagattcg cttaagaaga
     2041 gaatccgggt ttgtagctca gctggttaga gcacacgctt gataagcgtg gggtcggagg
     2101 ttcaagtcct cccagaccca ccaagaacgg gggcatagct cagttggtag agcacctgct
     2161 ttgcaagcag ggggtcatcg gttcgatccc gtttgcctcc accaatactg tacaaatcaa
     2221 aacggaagaa tggaacagaa tccattcagg gcgacgtcac acttgaccaa gaacaaaatg
     2281 ctgatataat aatcagctcg ttttgatttg cacagtagat agcaatatcg aacgcatcga
     2341 tctttaacaa attggaaagc cgaaatcaac aaacaaagac aaagcgtttg ttttgatttt
     2401 ttattctttg caaaggataa aaatctctcg cgagagaaaa gaaaacaaac acagtatttg
     2461 ggtgatgatt gtatcgactt aatcctgaaa cacaaaaggc aggattaaga cacaacaaag
     2521 cagtaagctt tatcaaagta ggaaattcaa gtctgatgtt ctagtcaacg gaatgttagg
     2581 caaagtcaaa gaagttcttg aaatgataga gtcaagtgaa taagtgcatc aggtggatgc
     2641 cttggcgatg ataggcgacg aaggacgtgt aagcctgcga aaagcgcggg ggagctggca
     2701 ataaagcaat gatcccgcga tgtccgaatg gggaaaccca ctgcattctg tgcagtatcc
     2761 taagttgaat acatagactt agagaagcga acccggagaa ctgaaccatc taagtacccg
     2821 gaggaaaaga aatcaaccga gattccgcaa gtagtggcga gcgaacgcgg aggagcctgt
     2881 acgtaataac tgtcgagata gaagaacaag ctgggaagct tgaccatagt gggtgacagt
     2941 cccgtattcg aaatctcaac agcggtacta agcgtacgaa aagtagggcg ggacacgtga
     3001 aatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcatca tcgaccgata
     3061 gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg gaggggagtg aaacagaacc
     3121 tgaaacctga tgcatacaaa cagtgggagc gccctagtgg tgtgactgcg taccttttgt
     3181 ataatgggtc aacgacttac attcagtagc gagcttaacc gaatagggga ggcgtaggga
     3241 aaccgagtct taatagggcg atgagttgct gggtgtagac ccgaaaccga gtgatctatc
     3301 catggccagg ttgaaggtgc cgtaacaggt actggaggac cgaacccacg catgttgcaa
     3361 aatgcgggga tgagctgtgg ataggggtga aaggctaaac aaactcggag atagctggtt
     3421 ctccccgaaa actatttagg tagtgcctcg agcaagacac tgatgggggt aaagcactgt
     3481 tatggctagg gggttattgc aacttaccaa cccatggcaa actcagaata ccatcaagtg
     3541 gttcctcggg agacagacag cgggtgctaa cgtccgttgt caagagggaa acaacccaga
     3601 ccgccagcta aggtccccaa tgatagatta agtggtaaac gaagtgggaa ggcccagaca
     3661 gccaggatgt tggcttagaa gcagccatca tttaaagaaa gcgtaatagc tcactggtcg
     3721 agtcgtcctg cgcggaagat gtaacggggc tcaaatctat aaccgaagct gcggatgccg
     3781 gtttaccggc atggtagggg agcgttctgt aggccgatga aggtgcattg taaagtgtgc
     3841 tggaggtatc agaagtgcga atgttgacat gagtagcgat aaagcgggtg aaaagcccgc
     3901 tcgccgaaag cccaaggttt cctgcgcaac gttcatcggc gtagggtgag tcggccccta
     3961 aggcgaggca gaaatgcgta gtcgatggga aacaggttaa tattcctgta cttgattcaa
     4021 atgcgatgtg gggacggaga aggttaggtt ggcaagctgt tggaatagct tgtttaagcc
     4081 ggtaggtgga agacttaggc aaatccgggt cttcttaaca ccgagaagtg acgacgagtg
     4141 tctacggaca cgaagtaacc gataccacgc ttccaggaaa agccactaag cttcagtttg
     4201 aatcgaaccg taccgcaaac cgacacaggt gggcaggatg agaattctaa ggcgcttgag
     4261 agaactcagg agaaggaact cggcaaattg ataccgtaac ttcgggagaa ggtatgccct
     4321 ctaaggttaa ggacttgctc cgtaagccca ggagggtcgc agagaatagg tggctgcgac
     4381 tgtttattaa aaacacagca ctctgctaac acgaaagtgg acgtataggg tgtgacgcct
     4441 gcccggtgct ggaaggttaa ttgaagatgt gagagcatcg gatcgaagcc ccagtaaacg
     4501 gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc
     4561 gcacgaatgg cgtaacgatg gccacactgt ctcctcctga gactcagcga agttgaagtg
     4621 gttgtgaaga tgcaatctac ccgctgctag acggaaagac cccgtgaacc tttactgtag
     4681 ctttgcattg gactttgaag tcacttgtgt aggataggtg ggaggcttag aagcagagac
     4741 gccagtctct gtggagccgt ccttgaaata ccaccctggt gtctttgagg ttctaaccca
     4801 gacccgtcat ccgggtcggg gaccgtgcat ggtaggcagt ttgactgggg cggtctcctc
     4861 ccaaagcgta acggaggagt tcgaaggtta cctaagtccg gtcggaaatc ggactgatag
     4921 tgcaatggca aaaggtagct taactgcgag accgacaagt cgagcaggtg cgaaagcagg
     4981 acatagtgat ccggtggttc tgtatggaag ggccatcgct caacggataa aaggtactcc
     5041 ggggataaca ggctgattcc gcccaagagt tcatatcgac ggcggagttt ggcacctcga
     5101 tgtcggctca tcacatcctg gggctgtagt cggtcccaag ggtatggctg ttcgccattt
     5161 aaagtggtac gtgagctggg tttaaaacgt cgtgagacag tttggtccct atctgcagtg
     5221 ggcgttggaa gtttgacggg ggctgctcct agtacgagag gaccggagtg gacgaacctc
     5281 tggtgtaccg gttgtaacgc cagttgcata gccgggtagc taagttcgga agagataagc
     5341 gctgaaagca tctaagcgcg aaactcgcct gaagatgaga cttcccttgc ggtttaaccg
     5401 cactaaagag tcgttcgaga ccaggacgtt gataggtggg gtgtggaagc gcggtaacgc
     5461 gtgaagctaa cccatactaa ttgctcgtga ggcttgactc tatcatttga agaacttcaa
     5521 gagataaaag cttactgact gattcagtca ttaccgaata tattgattaa ggctttaccg
     5581 atttgtaaca gtttaagttt ggcggccata gcgagttggt cccacgcctt cccatcccga
     5641 acaggaccgt gaaacgactc agcgccgatg atagtgtggt tcttccatgc gaaagtaggt
     5701 cactgccaaa cacccattca gaaaaccccc gattattcgg gggtttttgc tttgcccgga
     5761 aaaaatgtcg gggatggcgg gacagtatc
//
LOCUS       DAWHRS010000134         5579 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWHRS010000134 DAWHRS010000000
VERSION     DAWHRS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.134"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..934)
                     /gene="murJ"
                     /locus_tag="WIB82_001743"
     CDS             complement(<1..934)
                     /gene="murJ"
                     /locus_tag="WIB82_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ3843010.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEAAEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAQDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGL"
     gene            1152..1850
                     /locus_tag="WIB82_001744"
     CDS             1152..1850
                     /locus_tag="WIB82_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ3843011.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1907..2911)
                     /locus_tag="WIB82_001745"
     CDS             complement(1907..2911)
                     /locus_tag="WIB82_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ3843012.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLHRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2981..5389
                     /locus_tag="WIB82_001746"
     CDS             2981..5389
                     /locus_tag="WIB82_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ3843013.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDVGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
BASE COUNT         1393 a         1690 c         1451 g         1045 t
ORIGIN      
        1 gcaaacccca gtcgagcagg gcggaaaact gttccgtatc ttggtttgcc gagtgtttgg
       61 acaaagtcgg cagcaaaatc gtaccgagtg ccgcccccag cacgccgctg ggcagctcca
      121 tcatgcggtc ggcgtaatac atccatgaaa cgctgcccga ttgcagataa gacgcgaaaa
      181 ttgtgttgat caccaaagaa acctgcgcca cgctcacgcc caaaatcgca ggcgccatct
      241 gtttcatcac gcggttgacc gccgcatctt tgaaactcag tttgggcagt ttcaaaaagc
      301 ccagtttcgc cagccagggc agttggaagc cgagttgcaa aatgccgccg acaaagaccg
      361 cccacgccag cgcggtaacg ggcggatcga aatacggcac gaaaaacagc gcgaatacga
      421 taaacgacac gttcagaaac gtgggcgtaa acgccggaat gccgaactta tgataagaat
      481 tgagtaccga gccgacaaat gaagacaggg aaatcaataa tatataagga aacgtaatcc
      541 gcagcaaatc gatggagagc tgaaatttgt cggcatcttg ggcaaaaccg ggtgcggaaa
      601 cataaatcac ccaaggcgcg gcaagtatgc ccagcgcggt aacgataacc agtacaaacg
      661 acagcatccc cgccacatgg cggataaaag cctccgccgc ctcttttgaa cgcgtttcct
      721 tgtattccgc caaaatcggc acaaacgctt gggcaaacgc cccctccgca aacacgcggc
      781 gaagcaggtt gggcagtttg aacgcgacaa aaaacgcatc cgtcgccata cccgcgccga
      841 atgcccgcgc aatgaccgta tcgcgcacaa atcccaaaac gcgcgacacc atcgtcaggc
      901 tgccgacttt tgccaaagct cccagcatat tcatcattgt tcctcaacag tcgtacccgt
      961 ctggggcaac ggcgcgtatt gtacgacaga aaccgcttca gacggcatcg ggtttgatgc
     1021 cgtctgaagc ggtttcctga aacgaaaacg tccttttccg gcggcaaact gtatcaatac
     1081 gcggaaatgc aataaaatag ccggattccg attgatttcc aacatctgtt tccaacatca
     1141 cggagaaccg tatgaaatcc agacaccttg ccctcggcgt tgccgccctg ttcgcccttg
     1201 ccgcgtgcga cagcaaagtc caaaccagcg tccccgccga cagcgcgcct gccgcttcgg
     1261 cagccgccgc cccggcaggg ctggtcgaag ggcaaaacta taccgtcctt gccaacccga
     1321 ttccccaaca gcaggcaggc aaagtcgaag tccttgagtt tttcggctat ttctgtccgc
     1381 actgcgccca cctcgaacct gttttaagca aacacgccaa gtcttttaaa gacgatatgt
     1441 acctgcgtac cgaacacgtc gtctggcaga aagaaatgct gacgctggca cgcctcgccg
     1501 ccgccgtcga tatggctgcc gccgacagca aagatgtggc gaacagccat attttcgatg
     1561 cgatggttaa tcagaaaatt aagctgcaag agccggaagt cctcaaaaaa tggctgggcg
     1621 aacaaaccgc ctttgacggc aaaaaagtcc ttgccgctta cgaatctccc gaaagccagg
     1681 cgcgcgccga caaaatgcag gagctgaccg aaaccttcca aatcgacggt acgcccacgg
     1741 ttatcgtcgg cggtaaatat aaagtcgaat ttgccgactg ggagtccggt atgaacacca
     1801 tcgacctttt ggcggacaaa gtacgcgaag aacaaaaagc cgcgcagtaa gcccgtttga
     1861 aaaatgccgt ctgaaacttg gttttcagac ggcattttga ttgggtttaa aacgtaaagc
     1921 ccgtttccag ttcttcatcg ccgaccagtt cgaccaagag cgcgtagagc ggggcgagtt
     1981 cggcataacg gcgcgatacg cggtgcagat agtttaagaa acgcgggatt tccggacggt
     2041 atttgtcttt gccgtcgcgg tagtacaggc gtgcgaagat gcctgcaacc ttcaagtgcc
     2101 gctgcacgcc catccattcg aaccagcggt aaaactcgtc aaacgcttcg gggacgggca
     2161 ggccggcagc ccgcgccttt tcccagtagc ggataaccaa gtccaagaca aattcttctt
     2221 cccattcgat aaaggcatcg cgcaacagcg acaccaaatc gtaggaaatc gggccgtaaa
     2281 gcgcgtcttg gaagtctaaa acacccggcc tgccgcgcgt cagcatcagg ttgcggacga
     2341 taaagtcgcg gtgcacatag actttgggct gcgccaacag gggcggcagc agcgtatcga
     2401 cggtctgctg ccaaagttgg cgttgtttga atgttaattc gcgccccaat tcttttgcga
     2461 caaaccattc cgggaacagg ttgatttcgc gcagcatcgt ttcacggtca tattcgggca
     2521 aaaccccttc acggcttgcc ttctgcaatt cgaccagctc gccgattgcc tccaaaagca
     2581 gggctttgtg cgccgtttcg ccctgttcct gaagcatcgc ggtcaaaaac gtcgtattgc
     2641 ccaagtcgtt caataccaca aaccccagat ccgtgtccgc gtgcaatacc tgcggcacat
     2701 tgaccatgtc aaacagtttc tgcactttca aataaggtgc gacactcatc ttgtcgggcg
     2761 gtgcatccat gcagacgaca ctgctgccgt ctgaaaacgt tgcacggaaa tagcggcgga
     2821 aatcagcatc cgccgccgca aaagtcagat cgaagtcccg ttcgggataa acggtctgaa
     2881 gccaattttt cagtttgatt tgtcgttgca taacagtacg aaagcatttc aggttacaat
     2941 aaacgctatt ctaactggca aaccgacttg aggggcgatt ttggctcgtt tattttcact
     3001 caaaccactg gtgctggcat tgggcctctg cttcggcacg cattgcgccg ccgccgatgc
     3061 cgttgcggcg gaggaaacgg acaatccgac cgccggagga agcgttcgga gcgtgtccga
     3121 acccatacag cctaccagcc tgagcctcgg ttcgacctgc ctgttttgca gtaacgaaag
     3181 cggcagcccc gagagaaccg aagccgccgt ccgaggcagc ggcgaagcat ccatccccga
     3241 agactatacg cgcattgttg ccgacaaggt ggaaggacag tcgcaggtgc aggtgcgtgc
     3301 cgaaggcaac gtcgtcgtcg aacgcaaccg gacgaccctc aataccgatt gggcggatta
     3361 cgaccagtcg ggcgacaccg ttaccgcagg cgaccggttc gccctccaac aggacggtac
     3421 gctgattcgg ggcgaaaccc tgacctacaa tctcgagcag cagaccgggg aagcgcacaa
     3481 cgtccgtatg gaaaccgaac acggcggacg gcggctgcaa agcgtcagcc gcaccgccga
     3541 aatgttgggc gaagggcatt acaaactgac ggaaacccaa ttcaacacct gttccgccgg
     3601 cgatgtcggc tggtatgtca aggccgcttc cgtcgaagcc gatcgggaaa aaggcatagg
     3661 cgttgccaaa cacgccgcct tcgtgttcgg cggcgttccc attttctaca ccccttgggc
     3721 ggacttcccg cttgacggca accgcaaaag cggcctgctt gttccctcac tgtccgccgg
     3781 ttcggacggc gtttcccttt ccgttcccta ttatttcaac cttgccccca atctcgatgc
     3841 cacgttcgcg cccagcgtga tcggcgaacg cggcgcggtc tttgacgggc aggtacgcta
     3901 cctgcggccg gattatgccg gccagtccga cctgacctgg ctgccgcacg acaagaaaag
     3961 cggcaggaat aaccgctatc aggcgaaatg gcagcatcgg cacgacattt ccgacacgct
     4021 tcaggcgggt gtcgatttca accaagtctc cgacagcggc tactaccgcg acttttacgg
     4081 caacaaagaa atcgccggca acgtcaacct caaccgccgt gtatggctgg attatggcgg
     4141 cagggcggcg ggcggcagcc tgaatgccgg cctttcggtt ctgaaatacc agacgctggc
     4201 aaaccaaagc ggctacaaag acaaaccgta tgccctgatg ccgcgccttt ccgccgattg
     4261 gcgcaaaaac accggcaggg cgcaaatcgg cgtgtccgca caatttaccc gcttcagcca
     4321 cgacagccgc caagacggca gccgcctcgt cgtctatccc gacatcaaat gggatttcag
     4381 caacagctgg ggttacgtcc gtcccaaact cggactgcac gccacctatt acagcctcaa
     4441 ccgcttcggc agccaagaag cccgacgcgt cagccgcact ctgcccattg tcaacatcga
     4501 cagcggcgca acttttgagc ggaatacgcg gatgttcggc ggcggagtcc tgcaaaccct
     4561 cgagccgcgc ctgttctaca actatattcc tgccaaatcc caaaacgacc tgcccaattt
     4621 cgattcgtcg gaaagcagct tcggctacgg gcagctcttt cgcgaaaacc tctattacgg
     4681 caacgacagg attaacaccg caaacagcct ttccgccgcc gtgcaaagcc gtattttgga
     4741 cggcgcgacg ggggaagagc gtttccgcgc cggcatcggt cagaaattct atttcaagga
     4801 tgatgcggtg atgcttgacg gcagcgtcgg caaaaaaccg cgcaaccgtt ccgactgggt
     4861 ggcatttgcc tccggcagca tcggcagccg cttcatcctc gacagcagca tccactacaa
     4921 ccaaaacgac aaacgcgccg agaactacgc cgtcggtgca agctaccgtc ccgcacaggg
     4981 caaagtgctg aacgcccgct acaaatacgg gcgcaacgaa aaaatctacc tgaagtccga
     5041 cggttcctat ttttacgaca aactcagcca gctcgacctg tccgcacaat ggccgctgac
     5101 gcgcaacctg tcggccgtcg tccgttacaa ctacggtttt gaagccaaaa aaccgataga
     5161 ggtgctggcg ggtgcggaat acaaaagcag ttgcggctgc tggggcgcgg gcgtgtacgc
     5221 ccaacgctac gttaccggcg aaaacaccta caaaaacgct gtctttttct cacttcagtt
     5281 gaaagacctc agcagtgtcg gcagaaaccc cgcagacagg atggatgtcg ccgttcccgg
     5341 ctacatcccc gcccactctc tttccgccgg acgcaacaaa cgaccctgac cgtcggaaac
     5401 ccggcaggag caccgttccc gcacaagacg gcattccacc gacaacccca aacccgccat
     5461 caaaggtagg attcaaacga taaggaaaga atgatgaaaa tcaaagccct gatgattgcc
     5521 gccgcattgc tggcagcagc cgatgtccac gccgcaccgc aaaaggcaaa aaccgcatc
//
LOCUS       DAWHRS010000135         5426 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWHRS010000135 DAWHRS010000000
VERSION     DAWHRS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.135"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(97..2928)
                     /gene="lbpA"
                     /locus_tag="WIB82_001747"
                     /gene_synonym="tbpA"
     CDS             complement(97..2928)
                     /gene="lbpA"
                     /locus_tag="WIB82_001747"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ3843014.1"
                     /translation="MNRRHTFPLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIKRKTREPFFSVEGERESK
                     PVAKLAGYGKYLNNQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGNDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2925..5096)
                     /locus_tag="WIB82_001748"
     CDS             complement(2925..5096)
                     /locus_tag="WIB82_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3843015.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGPSVETTPVNRPAVGAAMRLPRRNTAFHREDGTVIPDSKQAEEQLSFKEG
                     DVLFLYGSKENKLQQLKSEIHKRDPDVEIRTSENENKKYDYKFVDAGYVYVKGKDEIK
                     WTSDHKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYFGNWQYMTDVKRHRAGKAVGI
                     DNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKILKGELIKNQYVRDRNGP
                     YNPLTIYDITATLDGNRFTGSAKVNTKLKTSHADKEHLFFHDDADQRLEGGFFGDNGE
                     ELAGRFISNDKSVFGVFAGKQKTETANASDTNPALPSGKHTKILDSLKISVDEATDSN
                     ARKFAISSMPDFGHPDKLLVEGREISLVKDTQTIDLADGRKMTVSACCDFLTYVKLGR
                     IKTDRPASKPKAEDENSEDEIGESEENEEDLVAEEENTEDEVVEDEDREEDEVSEDGN
                     SEDEEEIAEEDDDEAEEEEVEETEEESPVEESGGDSGSILPTPEAPKGRDIDLFLKGI
                     RTAEADIPKTGTAHYTGTWEARIGVPDKKDDQLYGTTFIQKDSYANQGAKAQFTVDFG
                     KKSISGTLTEKNDTHPAVYIEKGVIDGNGFHATARTRDNGIDLSGQGSTNPQRFEANN
                     LLVTGGFYGPQAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1168 a         1450 c         1258 g         1550 t
ORIGIN      
        1 cttgtttata tcgaatcaaa aaaacctgca ccttaatcag ttggcggttt agtccgactt
       61 ttggggtgca gatcaagctt tcagacggta tttcctttaa aacttcattt cgagcgcgag
      121 gctgaagttc ctgcctggtg cggcatacct tccatagttg ctgtcgccgc cgtgccggtt
      181 tgccgtgctt tccgcggtct ggcgcaagga ttcccaagta acgtagcggt agttgccgat
      241 attgtagata gccgccctca aggtcagccg ttttttcaga ttcagatagg cggaaacgtc
      301 tgccgtcgac caagaagacg acgctctttt tgtcgaatat cgtttttgat cgcctgccag
      361 ataagcaagc tcgtcagggt ttttcccttt ggaataggtc agcataatgt ttgcgcccca
      421 tttcccctct ggctggtcgt atccgaaccc caaaacataa cgcgacggct gtaccgcatc
      481 caaagcatag ctgcggaggg acagtcccgg ccggttggat accgatttcg gtttgatgcg
      541 gttgtacgcc aaagtcgtgt acaaaccttc gggcagtttg ccatacacgc cgttccagtc
      601 gatttttccc aatatattaa cgccttgaag cgacatattt tgggcattgt aataatcgcg
      661 tatatcaatc tctgtcaatt gtcctgcctg attcggcaat ttggttttgt gatcggcaac
      721 ggcaatcata tcggtataac ggttgcggaa gctgctgatt tccaaaaagc cgaaatcgcc
      781 cttccactgc aaaccgattt cgcggttggc tgccttttcc gatttcaggg cgggacgctg
      841 ccagcctttc ggataatcgt gataaatgtc tatcccgaaa agttcttgga atgagggcgt
      901 tctgaagccg ctggaggcgc ggtaagacaa agaaaaatgc cggttcggtt tgaacacgat
      961 gccgctgttc cacgaacggt caacataccg cccgctgcgg acgagttctt ccgacgtggt
     1021 gaagtttttc cggtcatacc tgccacccaa gctgaaatca aaatatttgc cgattgaaaa
     1081 acggtcgttc aaagaaatat ggatattgct gccgttgatt tttcttggca cgcatttgcg
     1141 ggaacgcagg gtttcgatgt agccgcagac cgacccttcg acgacttcgg gcttacccaa
     1201 acggtactta tcttgattct tttcatcgaa tcccgtggat tccgaaatcc ttgccgcatt
     1261 gtgggaaagc tgttcagggc gggagatcgc gttggaagca tcgtaaccga agcccaaagt
     1321 cagatggtgt ttcgtccatt tgtttttcag cgacttctca aacgaggcat tcaaaacatt
     1381 gtgctgttcg cggtagtgga aacggtcgct gctgtcgtag gaatacggtt tgtccgccga
     1441 cgcgcggcag gatttgtcca cagcaggata cacggcacaa ttcagcttca gcgtgttgtt
     1501 gtcggttgcc acgccctgtt tgtcaaacga caacaccgcc ttgtccgccc aattgtcaga
     1561 atacgcttcg ttttcataac ggtacagcaa acccatacgg cggcggcggt gatgttcgtc
     1621 aataaatttg gtgcgggaat atttcaaacc tatgcccctg accaactttt tatcgccctt
     1681 ccactcttct atattcggca caaaatacaa gccgtcgcgg aaatcgttgc cgtcgtacac
     1741 cccgctcttg tctctaaact tttccgcctc gtccgtaccg taatactgtt tttccgtcat
     1801 atcgcggatg tcgtaacgct gtttggtatc ctcaaacaca ccgccgacat aatgcctgcc
     1861 gccgaagcgg tagcccagct tggcaagcca cgaaccgctg cggtaatcca tcggatcggg
     1921 caatatcctg ccgccgcccg tgtaagcttg ggcggacaga ttttcgtggc gcgcctgcgc
     1981 ctcccgcacc tgcgcctctt cttcagcact taaaggctga ttttgttcaa tacgttcttt
     2041 tacccagcgg ttgagctggt tgttcaaata tttcccgtag cccgccaatt ttgccacggg
     2101 cttggattca cgctcgccct ctactgagaa aaatggctct cttgtcttgc gtttaatatc
     2161 gtatgtctgg cggaacgcgt ccaaacggtt tatgccgtat gccaccccgt ccgcaatatc
     2221 gccgtgcggg cgcgtttccc gcccttggcg ttcggtacgg attagcagcc cttcccaacc
     2281 gtctttgctg aaccccgcgc cgagcgactt cataaattgg cggtttttac tgccgtaggc
     2341 ggtttttgcc tgtatccccc agcttttgcc gtctgaaatc aggtctgccg cctctttggt
     2401 gcggaaggcg accgcgccgc cgagtgcgcc gctgccgtga tcggacgaac cggcaccttt
     2461 gtcgatttcc accgtgctga tgttttcata ttcgatttcg ttgattgcac cgctgccgcc
     2521 gcgtccgccg tatccgctca acgatccctg cacggtaaac gcctgtattt gggcaacgcc
     2581 gtcaaccgaa accgccacac ggtttttatc cacgccgcgt atcgagtagc cgccgctcgc
     2641 gccgttgccc tgttcgacaa ccgccacgcc cggatcgtag cgcgtcaggt cgcggatacc
     2701 gagtacctgt tctttgttca acgtttccga cgttttgacg attttgccca aaccggtcgc
     2761 ctctttcgat cgccgtccca ctttggcggc acggacggta acctctttca gggattgggt
     2821 ctgcgccgca tcaggtgccg ccgtttcagg gtttgccgca tatgacggga aggcggcggc
     2881 aacagccaag gcagtcaggg taagtgggaa tgtatgcctt ctgttcatcg tgttgcctcc
     2941 ttgtcatctt tcttcgcacc gaataccgcg ccgaatttcc ggtcgctgtc gataatatta
     3001 ccgcccaatt ccgccgcctg cggtccataa aagccgcctg ttacaagaag attgttggct
     3061 tcgaagcgct gcgggttggt cgaaccctgc ccggaaaggt caataccgtt atcccgagtg
     3121 cgtgctgtcg cgtggaaacc gttgccatca atcacacctt tttcaatata aacagcgggg
     3181 tgtgtatcat ttttttctgt caacgttccg gaaatcgatt tcttgccgaa atcaacggta
     3241 aattgtgctt ttgccccttg atttgcatag ctatcctttt gaatgaacgt agtgccatat
     3301 aactgatcgt ctttcttatc cggtacaccg atacgcgctt cccaagtgcc ggtataatgc
     3361 gccgttccgg tttttggaat gtcggcttcc gccgtgcgga tacctttcag gaaaaggtcg
     3421 atgtccctgc ctttaggggc ttccggagtg ggcaggatgc tgcctgaatc gccaccgctt
     3481 tcttccaccg gcgattcttc ttcggtttct tcaacttcct cctcttcggc ttcatcatca
     3541 tcttcttcgg cgatttcttc ttcgtcttca ctgttaccat cttcggaaac ttcgtcttct
     3601 tctctgtctt catcttctac gacttcgtct tccgtgtttt cttcttcagc gaccaaatcc
     3661 tcttcatttt cctcgctttc accaatttca tcttcggaat tttcatcttc cgcctttggt
     3721 ttacttgccg ggcggtcggt ttttatccgt ccgagtttca cataggtcaa aaagtcgcaa
     3781 caagcactga cggtcatttt cctgccgtcg gcaagatcaa tggtttgagt atctttgacc
     3841 aaggagattt cacgcccttc gacaagaagc ttgtcgggat gaccaaaatc gggcatagag
     3901 gaaatggcaa acttacgggc attgctatca gtcgcctcgt caacggaaat ttttagagaa
     3961 tccaagattt tggtgtgttt tccagacggc agggcaggat ttgtatctga tgcgtttgct
     4021 gtctctgttt tttgtttgcc tgcgaatacg ccgaatacgc ttttgtcgtt gctgataaac
     4081 cgtccggcaa gctcttctcc gttatcaccg aaaaaaccgc cctcaagccg ctgatcggca
     4141 tcgtcatgga aaaacaaatg ctctttatca gcgtggctcg tcttcaactt ggtgttaact
     4201 ttggcactgc cggtaaagcg gttgccgtcc aatgttgcag taatgtcgta aatggtcagc
     4261 ggattatagg gaccatttct atctcgcaca tactgatttt taatcagctc gccctttagg
     4321 atttttttgt cgaaatcaac cgtatatttg gcaggatgct tttccctttc gtcgacatcc
     4381 ttagccgcat aagaagttgc accaacatcg ttaccataaa atgtgatata acccaaattg
     4441 tcaatgccaa ccgccttacc tgctcgatga cgtttgacat cggtcatata ttgccagtta
     4501 ccaaaatatt ccaccgttcc cgcactcggt aaagattggg aaggacgttc tccggaataa
     4561 tatacaaaac cgtcataacc taagcggttg gaaaactgct tgtgatctga agtccactta
     4621 atttcatctt ttccctttac atatacataa cctgcatcta caaatttata atcatatttt
     4681 ttattttcat tttctgatgt cctaatttct acatcgggat cacgtttatg aatttcgctt
     4741 ttaagttgtt gaagtttatt ttcttttgaa ccgtataaaa acagaacatc accttcttta
     4801 aacgacagct gttcttctgc ttgtttgcta tcgggaatta ccgtgccatc ttcacgatga
     4861 aaagcagtat tccgccttgg cagccgcatt gccgcaccga cggcgggccg gttgaccggc
     4921 gtggtttcta ccgaaggccc ggcagggggg ggagtgggaa cgtccttaga cttgaaagtg
     4981 acggggtacg cggtcggcgt tgattcgaca acaggctgca cgccgaaatt gccgccgata
     5041 caagatgcta aaagtaaggg caacaagaca atgccgccat aattcggttt acacatccct
     5101 acttttcctc ctatttgatt aataataatt atcattatat taatatgtac agataatatc
     5161 aagccgtttt tatagtgaat taacaaaaat ccggacaagg cgacgagccg cagacagtac
     5221 agatacattc cgtcattccc acgaacctac atcccgtcat tcccacgaac ctacattccg
     5281 tcattcccac gaaagtggga atccagttcg ttcggtttcg cttgttttaa gtttcgggta
     5341 acttccactt cgtcattccc gcgcaggcgg gaatccagtg tgttgagttt cagctattta
     5401 gaataaattt tgaaactcta atcgcg
//
LOCUS       DAWHRS010000136         5401 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWHRS010000136 DAWHRS010000000
VERSION     DAWHRS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.136"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            102..2246
                     /locus_tag="WIB82_001749"
     CDS             102..2246
                     /locus_tag="WIB82_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3843016.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVSSEKPQAQKDQGGYGFAMRFKRRNWHPQANPKEDEIKLSENDWEATGLPGNPKN
                     LPERQKSVIEKVKTGSDSNIYSSPYLTQSNHQNGSANQPKNEVTDYKEFKYVYSGWFY
                     KHAKLEIIKENNLIKGAKSGDDGYIFYHGEKPSRQLPVSGEVTYKGVWHFVTDTKQRQ
                     KFNDILGTSKKQGDRYSGFPGDDGEEYSNKNEATLQGSQEGYGFTSNLKVDFNKKKLT
                     GELIRNNRVTNATANDKYTTQYYSLEAQVTGNRFNGTATATDKPKKDGETKEHPFVSD
                     SSSLSGGFFGPKGEELGFRFLSDDKKVAVVGSAKTKDKDANGNTEAASGGTGAAASGG
                     AAAMPSENGKLTTVLDAVELTHGGKAIKNLDNFSNAAQLVVDGIMIPLLPEASESGNT
                     NQGTNGGTAFTRKFDYTPKSDEKDTQAGTAANGDQAASNTAGDTNGKTKTYEVEVCCS
                     NLNYLKYGLLTRKTAGNTGEGGNGSPTAAAQTAQGAQSMFLQGERTDEKEIPSEQNVV
                     YRGSWYGHIASSTSWSGNASDKEGGNRAEFTVNFGEKKITGTLTAENRQAATFTIDGK
                     IEGNGFSGTAKTAELGFDLDQKNTTRTPKAYITNAKVQGGFYGPKAEELGGWFAYSDD
                     KQTKNATDASGNGNSASSATVVFGAKRQQPVQ"
     gene            2333..5098
                     /gene="tbpA"
                     /locus_tag="WIB82_001750"
     CDS             2333..5098
                     /gene="tbpA"
                     /locus_tag="WIB82_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ3843017.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDDNQYAHFVVEEECESGSYEKCKSKP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDANKKQAGSLRGNGKYAGNHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYYQHANRAYSSNTPPKNGNGGSNANPNANPNGGKDKPYWVSIGGGNVVTGQ
                     ICRLGNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVST
                     GTHRTLSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSF
                     NKEAGIVFKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARIT
                     GINILGKIDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYV
                     VGLGYDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDV
                     SGYYTIKKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYT
                     FSLEMKF"
BASE COUNT         1568 a         1367 c         1362 g         1104 t
ORIGIN      
        1 gattttggtg tattccctaa aaaaataaaa aagtatttgc aaatttgtta aaaataaata
       61 aaataataat ccttatcatt ctttaattga attgggttta tatgaacaat ccattggtaa
      121 atcaggctgc tatggtactg cctgtgtttt tgttgagtgc ttgtttgggc ggaggcggcg
      181 gcagtttcga tcttgattct gtcgataccg aagccccgcg tcccgcgcca aagtatcaag
      241 atgtttcttc cgaaaaaccg caagcccaaa aagaccaagg cggatacggt tttgcaatgc
      301 gcttcaagcg gcggaattgg catccgcagg caaatcctaa agaagatgag ataaaacttt
      361 ctgaaaatga ttgggaggcg acaggattgc caggcaatcc caaaaactta cctgagcgac
      421 agaaatcggt tattgaaaaa gtaaaaacag gcagcgacag caatatttat tcttccccct
      481 atctcacgca atcaaaccat caaaacggca gtgcaaacca accaaaaaac gaagtaacag
      541 attataaaga gttcaaatat gtttattccg gttggtttta caaacacgct aaactcgaaa
      601 tcataaaaga aaacaactta attaagggtg caaagagcgg cgacgacggt tatatctttt
      661 atcacggtga aaaaccttcc cgacaacttc ccgtttctgg agaagttacc tacaaaggcg
      721 tatggcattt tgtaaccgat acgaaacaga gacaaaaatt taacgatatt cttggaacct
      781 caaaaaaaca aggcgacagg tatagcggat ttccgggtga tgacggcgaa gaatattcca
      841 ataaaaatga agcgacttta caaggcagtc aagagggtta tggttttacc tcaaatttaa
      901 aagtggattt caataagaaa aaattgacgg gtgaattgat acgcaataat agagttacaa
      961 acgctactgc taacgataaa tacaccacgc aatattacag ccttgaggct caagtaacag
     1021 gcaaccgctt caacggcacg gcaacggcaa ccgacaaacc caaaaaagac ggcgaaacca
     1081 aggaacatcc ctttgtttcc gattcgtctt ctttgagcgg cggctttttc ggcccgaagg
     1141 gtgaggaatt gggtttccgc tttttgagcg acgataaaaa agttgccgtt gtcggcagcg
     1201 cgaaaaccaa agacaaagac gcaaatggca atactgaggc ggcttcaggc ggcacaggtg
     1261 cggcagcatc gggcggtgcg gcagctatgc cgtctgaaaa cggtaagctg accacggttt
     1321 tggatgcggt cgagctgacg cacggcggca aagcaatcaa aaatctcgac aacttcagca
     1381 atgccgccca actggttgtc gacggcatta tgattccgct cctgcccgag gcttccgaaa
     1441 gcgggaatac caatcaaggt acaaacggcg gaacagcctt tacccgcaaa tttgattaca
     1501 cgccgaaaag cgatgaaaaa gacacccaag caggtacggc ggcgaatggc gatcaagccg
     1561 cttcaaatac ggcaggtgat accaatggca aaacaaaaac ctatgaagtc gaagtctgct
     1621 gttccaacct caattatctg aaatacgggt tgctgacgcg caagactgcc ggcaatacgg
     1681 gggaaggcgg caacggcagc ccaaccgccg ccgcccaaac ggcacagggt gcacaaagta
     1741 tgttcctcca aggcgagcgc accgatgaaa aagagattcc aagcgagcaa aacgtcgttt
     1801 atcgggggtc ttggtacggg catattgcca gcagcacaag ctggagcggc aatgcttctg
     1861 ataaagaggg cggcaacagg gcggaattta ctgtgaattt tggcgagaaa aaaattaccg
     1921 gcacgttaac cgctgaaaac aggcaggcgg caacctttac cattgatggt aagattgagg
     1981 gcaacggttt ttccggtacg gcaaaaactg ctgaattagg ttttgatctc gatcaaaaaa
     2041 ataccacccg cacgcctaag gcatatatca caaacgccaa ggtgcagggc ggtttttacg
     2101 ggcccaaagc cgaagagttg ggcggatggt ttgcctattc ggacgataaa caaacgaaaa
     2161 atgcaacaga tgcatccggc aatggaaatt cagcaagcag tgcaactgtc gtattcggtg
     2221 cgaaacgcca acagcctgtg caataagcac ggttgccgaa caatcaagaa taaggcttca
     2281 gacggcatcg ctccttccga tgccgtctga aagcgaagat tagggaaaca ctatgcaaca
     2341 gcaacatttg ttccgattaa atattttatg cctgtcttta atgaccgcgc tgcccgttta
     2401 tgcagaaaat gtgcaagccg gacaagcaca ggaaaaacag ttggatacca tacaggtaaa
     2461 agccaaaaaa cagaaaaccc gccgcgataa cgaagtaacc ggcctgggca aattggtcaa
     2521 aaccgccgac accctcagca aggaacaggt actcgatatc cgcgacctga cgcgttacga
     2581 ccccggcatc gccgttgtcg aacaggggcg cggcgcaagc tctggctact cgatacgcgg
     2641 tatggacaaa aaccgcgttt ccttgacggt ggacggcttg gcgcaaatac agtcctacac
     2701 cgcgcaggcg gcattgggcg ggacgaggac ggcgggcagc agcggcgcaa tcaatgaaat
     2761 cgagtatgaa aacgtcaagg ctgtcgaaat cagcaaaggc tcaaactcgg tcgaacaagg
     2821 cagcggcgca ttggcgggtt cggtcgcatt tcaaaccaaa accgccgacg atgttatcgg
     2881 ggaaggcagg cagtggggca ttcagagtaa aaccgcctat tccggcaaaa accgggggct
     2941 tacccaatcc atcgcgctgg cggggcgcat cggcggtgcg gaggctttgc tgatccacac
     3001 cgggcggcgc gcgggggaaa tccgcgccca cgaagatgcc ggacgcggcg ttcagagctt
     3061 taacaggctg gtgccggttg aggacgacaa ccaatacgct catttcgtgg tcgaagaaga
     3121 atgcgaaagc gggagttatg aaaagtgtaa aagcaaaccg aaaaaagatg ttgtcggcaa
     3181 agacgaacgt caaacggttt ccacccgaga ctacacgggc cccaaccgct tcctcgccga
     3241 tccgctttca tacgaaagcc ggtcgtggct gttccgcccg ggttttcgtt ttgaaaacaa
     3301 acggcactac atcggcggca tactcgaaca cacgcaacaa actttcgaca cgcgcgatat
     3361 gacggttccg gcattcctga ccaaggcggt ttttgatgca aataaaaaac aggcgggttc
     3421 tttgcgcggc aacggcaaat acgcgggcaa ccacaaatac ggcggactgt ttaccaacgg
     3481 cgaaaacggt gcgctggtgg gcgcggaata cggtacgggc gtgttttacg acgagacgca
     3541 caccaaaagc cgctacggtt tggaatatgt ctataccaat gccgataaag acacttgggc
     3601 ggattatgcc cgcctctctt acgaccggca gggcatcggt ttggacaatc attttcagca
     3661 gacgcactgt tctgccgacg gttcggacaa atattgccgc ccgagtgccg acaagccgtt
     3721 ttcctattac aaatccgatc gcgtgattta cggggaaagc cacaggctct tgcaggcggc
     3781 attcaaaaaa tccttcgata ccgccaaaat ccgccacaac ctgagcgtga atctcgggtt
     3841 tgaccgcttt ggctctaatc tccgccatca ggattattat tatcaacatg ccaaccgcgc
     3901 ctattcgtcg aacacgcctc ctaaaaacgg caacggcggc tcaaacgcca accccaacgc
     3961 caaccccaac ggcggcaagg acaaacccta ttgggtcagc ataggcgggg gaaatgtggt
     4021 tacggggcaa atctgccgct tgggcaacaa tacttatacg gactgcacgc cgcgcagcat
     4081 taacggcaaa agctattacg cggcagttcg ggacaatgtc cgtttgggca ggtgggcgga
     4141 tgtcggcgcg gggttgcgct acgactaccg cagcacgcat tcggacgacg gcagcgtttc
     4201 caccggcacg caccgcaccc tgtcctggaa cgcaggcatc gtcctcaaac ctgccgactg
     4261 gctggatttg acttaccgca cttcaaccgg cttccgcctg ccctcgtttg cggaaatgta
     4321 cggctggcgg tcgggcgata aaataaaagc cgtcaaaatc gatccggaaa aatcgttcaa
     4381 caaagaagcc ggcatcgtgt ttaaaggcga tttcggcaac ttggaggcaa gttggttcaa
     4441 caatgcctac cgcgatttga ttgtccgggg ttatgaagcg caaattcaaa acggcaaaga
     4501 aacagccaaa ggcgacccgg cttacctcaa tgcccaaagc gcgcggatta ccggcatcaa
     4561 tattttgggc aaaatcgatt ggaacggcgt atgggataaa ttgcccgaag gttggtattc
     4621 tacatttgcc tataatcgtg tccatgtccg cgacatcaaa aaacgcgcag accgcaccga
     4681 tattcaatca catctgtttg atgccatcca accttcgcgc tatgtcgtcg gcttgggcta
     4741 tgaccaaccg gaaggcaaat ggggtgtgaa cggtatgctg acttattcca aagccaagga
     4801 aatcacagag ttgttgggca gccgtgcttt gctcaacggc aacagccgca atacaaaagc
     4861 caccgcgcgc cgtacccgcc cttggtatat tgtggatgtg tccggttatt acacgattaa
     4921 aaaacacttc accctccgtg cgggcgtgta caacctcctc aactaccgct atgttacttg
     4981 ggaaaatgtg cggcaaactg ccggcggcgc agtcaaccaa cacaaaaatg tcggcgttta
     5041 caaccgatat gccgcccccg gtcgcaacta cacatttagc ttggaaatga agttttaaac
     5101 gtccaaacgc cgcaaatgcc gtctgaaagg cttcagacgg cattttttac acaattccca
     5161 ccgtttccca tcatccccga tacaccgtaa tcctgaaatc cgtcattccc gagcaggcgg
     5221 gaatccagtc cgttcggttt aggttttttt gaggtttcgg gtaactctaa accgtcattc
     5281 ctacgaaaac agaaaatcaa aaacagaaac ctcaaatccc gtcattcccg cgcaggcggg
     5341 aatctagggc gtaaaatcta aagaaaccgt tttatccgat aagtttccgc accgacagac
     5401 t
//
LOCUS       DAWHRS010000137         5391 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWHRS010000137 DAWHRS010000000
VERSION     DAWHRS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.137"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(40..996)
                     /locus_tag="WIB82_001751"
     CDS             complement(40..996)
                     /locus_tag="WIB82_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3843018.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGDLADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            1232..2197
                     /locus_tag="WIB82_001752"
     CDS             1232..2197
                     /locus_tag="WIB82_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3843019.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAGKE"
     gene            2742..3065
                     /locus_tag="WIB82_001753"
     CDS             2742..3065
                     /locus_tag="WIB82_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ3843020.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            3179..5356
                     /locus_tag="WIB82_001754"
     CDS             3179..5356
                     /locus_tag="WIB82_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ3843021.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQKSTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         1229 a         1692 c         1515 g          955 t
ORIGIN      
        1 aggttgtttc aaatcaaaat atctatgccg ccggccccat cagcacttct tcacatccga
       61 aggcaaaaat ccgtaatgcc gtctgaacgc ttcgttgaac cgtcccgcgt ggcggtagcc
      121 gcaaaagtgc atggcggctt ggacggtgct gccggattcg atgagggcga gcgcgtgttc
      181 cagccgcagg cggcgcaggc atccggcgac ggtttcgccg gtttgcgctt tgaaatagcg
      241 tttcaggtag cattcgttca gtccgacgcg gcgggcgatt tcggcgatgg tcagcggacg
      301 ggcgaattcg tgttgcagga tgtcggcggc ttcgtctatg cgccgacggc ggtaaccgtt
      361 gtcgtggcgg cggaaggtga agcgcaataa tcgggcggag agttccagcg cggcggcttc
      421 gtcggcaagc aggccgaagc cgtccgattc gaacgggcgt tgcagcagtg ggcaggccgc
      481 cgccgtcagt gccgctgcgt tttgcgccag ccgttgcagg gcgaatcggc ctattgtttg
      541 cggcgaaaac aggcgttcgt ccagcaagcc ttcgtcgtgc cagcggcgca gtttttccag
      601 cgaaaaatcc aaatgcagcg cgcacatgcc gctgttgtcg ggcagcaggg tttcggatac
      661 gtccgccaaa tcgccgcgta ccagccagat ttcgccggca gatgggcggt attccctgcc
      721 gcccatttgt aaccggttct gccccgacac catgacgaac aaggcgcagt tgtggctgaa
      781 attgtggatt tcggtgggaa acgcgcccgt tccgccgccg cgcatccgcg acaaggtgat
      841 gcccgagtcg aagcggttga tgcacatttc cagatgcaaa ccgggctgtt ttgcctgcgc
      901 aatgagcgcg ctgtcggaac agccgtccaa cgcccagccg gatttatcgg agcggacata
      961 ggtttggtac tggcggtaga tggcggcggt gttcatgatt ggataggaac gagttgtcta
     1021 acaaatgaat taaataggaa ttattaccaa taatcaagcg cagggattgg ttgaaacgga
     1081 aaaggtcgtc tgaaagggtg tttcagacga ccttttccgt atcgggaatt tgttttgccg
     1141 tatcgggaat tttgcgtttt gcggcgtggt ttctgcaggt tgtttgctta ataataaaca
     1201 ttcttattcg tatgcaaagg aaccgcacac cgtgaaaccg cgtttttatt gggcagcctg
     1261 cgccgtcctg ctgaccgcct gttcgcccga acctgccgcc gaaaaaactg tatccgccgc
     1321 atccgcatct gccgccacac tgaccgtgcc gaccgcgcgg ggcgatgccg ttgtgccgaa
     1381 gaatcccgaa cgcgtcgccg tgtacgactg ggcggcgttg gatacgctga ccgaattggg
     1441 cgtgaatgtg ggcgcaacca ccgcgccggt gcgcgtggat tatttgcagc ctgcatttga
     1501 caaggcggca acggtgggga cgctgttcga gcccgattac gaagccctgc accgctacaa
     1561 tcctcagctt gtcattaccg gcgggccggg cgcggaagca tatgaacagt tggcgaaaaa
     1621 cgcgaccacc atagatctga cggtggacaa cggcaatatc cgcaccagcg gcgaaaagca
     1681 gatggagacc ttggcgcgga ttttcggcaa ggaagcgcgc gcggcggaat tgaaggcgca
     1741 gattgacgcg ctgttcgccc aaacgcgcga agccgccaaa ggcaaaggac gcgggctggt
     1801 gctgtcggtt acgggcaaca aggtgtccgc cttcggcacg cagtcgcggt tggcaagttg
     1861 gatacacggc gacatcggcc taccgcctgt agacgaatct ttacgcaacg aggggcacgg
     1921 gcagcctgtt tccttcgaat acatcaaaga gaaaaacccc gattggattt tcatcatcga
     1981 ccgtaccgcc gccatcgggc aggaagggcc ggcggctgtc gaagtattgg ataacgcgct
     2041 ggtacgcggc acgaacgctt ggaagcgcaa gcaaatcatc gtcatgcctg ccgcgaacta
     2101 cattgtcgcg ggcggcgcgc ggcagttgat tcaggcggcg gagcagttga aggcggcgtt
     2161 tgaaaaggca gaacccgttg cggcggggaa agagtaggga gttgtctgaa aacggagctt
     2221 ccgaaggaag cgggggggtt tctgcgaagc taaagtacgg tttcaacgaa ttgaaaagca
     2281 gcctgtatgt tgaaaatacc gctcaagcaa acctgcggtt tgccgccctc tccctagccc
     2341 tctcccacag ggagagggga ttggggtgca ggctgtcttt aagcttcagg cgatttttaa
     2401 ctcttgttga agctgcgatt tcagaagctc cgttttagct tcgcagaaac tccgcttcct
     2461 tcgaaagctc cgttttcaga cgaccttttg gaacaccaca ggcacacgca tcgaacggct
     2521 gaatcaaagt tcagaccgat ggcagcccgc acccgagttt atgcggcaaa cagcgaggct
     2581 acggcaaccc gtcccctctc cctgtgggag agggttaggg agagggcggt aagccgcagg
     2641 cttgcatcaa agtcgataac gctttcgtta caactccgcc cactgaacga agccaaaacg
     2701 acaaaccgca tcgtaaacca cccaacccat aggagaaccc catgcaaaac gaaaccatca
     2761 acctgaaaca gcaccttgcc gccatcaaag aatactggca gcccgaaatc atcaaccgcc
     2821 acgggttcca attccgtttg gtcaaacttt tgggcgatta cggctggcat acgcacggat
     2881 acagcgacaa agtgctgttt gccgtggagg gcgacatggc ggtggacttc gccgacggcg
     2941 gcagcatgac gatacgcgag ggcgagatgg cggtcgtgcc gaagtcggtg tcgcaccgcc
     3001 cgcgttcgga aaacggctgc tcgttggtgc tgattgagtt gtccgacccg tccgaggccg
     3061 tctgaaaacg aagtttccga aggaagctga gtttctgcga agctaaaagc agcctgcacc
     3121 ttcaatcaat atgccgaaaa tacaacccca ccgcacacca acacacaaag gaaatcccat
     3181 gacacgcttc aaatattccc tgctgtttgc cgccctgttg cccgtgtacg cgcaggccga
     3241 tgtttctgtt tcagacgacc ccaaaccgca gaaaagcact gaattgccga ccatcaccgt
     3301 taccgccgac cgcaccgcga gttccaacga cggctacact gtttccggca cgcacacccc
     3361 gctcgggctg cccatgaccc tgcgcgaaat cccgcagagc gtcagcgtca tcacatcgca
     3421 acaaatgcgc gaccaaaaca tcaaaacgct cgaccgcgcc ctgttgcagg cgaccggcac
     3481 cagccgccag atttacggct ccgaccgcgc gggctacaac tacctgttcg cgcgcggcag
     3541 ccgcatcgcc aactaccaaa tcaacggcat ccccgttgcc gacgcgctgg ccgatacggg
     3601 caatgccaac accgccgcct atgagcgcgt agaagtcgtg cgcggcgtgg cggggctgct
     3661 ggacggcacg ggcgagcctt ccgccaccgt caatctggtg cgcaaacgcc cgacccgcaa
     3721 gccattgttt gaagtccgcg ccgaagcggg caaccgcaaa catttcgggc tgggcgcgga
     3781 cgtatcgggc agcctgaaca ccgaaggcac gctgcgcggc cgcctggttt ccaccttcgg
     3841 acgcggcgac tcgtggcggc agcgcgaacg tagccgcgat gccgaactct acggcatttt
     3901 ggaatacgac atcgcaccgc aaacccgcgt ccacgcaggc atggactacc agcaggcgaa
     3961 agaaaccgcc gacgcgccgc tcagctacgc cgtgtacgac agccaaggtt atgccaccgc
     4021 cttcggcccg aaagacaacc ccgccacaaa ttgggcgaac agccgccacc gtgcgctcaa
     4081 cctgttcgcc ggcatcgaac accgcttcaa ccaagactgg aaactcaaag ccgaatacga
     4141 ctacacccgc agccgcttcc gccagcccta cggcgtagca ggcgtgcttt ccatcgacca
     4201 caacaccgcc gccaccgacc tgattcccgg ttattggcac gccgacccgc gcacccacag
     4261 cgccagcgtg tcattgatcg gcaaataccg cctgttcggc cgcgaacacg atttaatcgc
     4321 gggtatcaac ggttacaaat acgccagcaa caaatacggc gaacgcagca tcatccccaa
     4381 cgccattccc aacgcctacg aattttcccg cacgggtgcc tacccgcagc ctgcatcgtt
     4441 tgcccaaacc atcccgcaat acggcaccag gcggcaaatc ggcggctatc tcgccacccg
     4501 tttccgcgcc gccgacaacc tttcgctgat actcggcggc agatacagcc gttaccgcac
     4561 cggcagctac gacagccgca cacaaggcat gacctatgtg tccgccaacc gtttcacccc
     4621 ctacacaggc atcgtgttcg acctgaccgg caacctgtct ctttacggct cgtacagcag
     4681 cctgttcgtc ccgcaatcgc aaaaagacga acacggcagc tacctgaaac ccgtaaccgg
     4741 caacaatctg gaagccggca tcaaaggcga atggcttgaa ggccgtctga acgcatccgc
     4801 cgccgtgtac cgcgcccgta aaaacaacct cgccaccgca gcaggacgcg acccgagcgg
     4861 caacacctac taccgcgccg ccaaccaagc caaaacccac ggctgggaaa tcgaagtcgg
     4921 cggccgcatc acgcccgaat ggcagataca ggcaggttac agccaaagca aaacccgcga
     4981 ccaagacggc agccgcctga accccgacag cgtacccgaa cgcagcttca aactcttcac
     5041 tgcctaccac tttgcccccg aagcccccag cggctggacc atcggcgcag gcgtgcgctg
     5101 gcagagcgaa acccacaccg accctgccac gctccgcatc cccaaccccg ccgccaaagc
     5161 ccgcgccgcc gacaacagcc gccaaaaagc ctacgccgtc gccgacatca tggcgcgtta
     5221 ccgcttcaat ccgcgcgccg aactgtcgct gaacgtggac aatctgttca acaaacacta
     5281 ccgcacccag cccgaccgcc acagctacgg cgcactgcgg acagtgaacg cggcgtttac
     5341 ctatcggttt aaataagatc gtctgaaaac ggagtttctg cgaagctata g
//
LOCUS       DAWHRS010000138         5306 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWHRS010000138 DAWHRS010000000
VERSION     DAWHRS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.138"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(63..623)
                     /locus_tag="WIB82_001755"
     CDS             complement(63..623)
                     /locus_tag="WIB82_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ3843022.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WIB82_001756"
     CDS             complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WIB82_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ3843023.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2243..3174
                     /locus_tag="WIB82_001757"
                     /pseudo
     CDS             2243..3174
                     /locus_tag="WIB82_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3332..3970)
                     /locus_tag="WIB82_001758"
     CDS             complement(3332..3970)
                     /locus_tag="WIB82_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ3843024.1"
                     /translation="MSKLTPREILSAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAS
                     HNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4151..>5306)
                     /locus_tag="WIB82_001759"
     CDS             complement(4151..>5306)
                     /locus_tag="WIB82_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ3843025.1"
                     /translation="TFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHL
                     ARGIKNGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFD
                     EISSIIPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYT
                     KEPFLIDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREG
                     CRIIEAPAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQ
                     DRGFKVALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVL
                     INETEWNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1212 a         1272 c         1462 g         1360 t
ORIGIN      
        1 ctaaatatct agcaaaaaag tgcggtcaaa attcaccgca cttttcgttt cgaacaatcg
       61 aattatttaa tcacgccaca tgccatacgt gggccgccac cgccaagtgg agctggatga
      121 tcggagtgat tatcaccacc cgtgtggatc ataatagagt gaccgcgaac atcatctaaa
      181 tgtttaagac gtggtgctaa aacaggattt gttgctgtgc catcatgcaa tacagttaat
      241 gcaggtaaat cacctaagtg tgcatcatct tgccatgggt aaccatgttg ttttgcacct
      301 ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca atttaccttc tttttctttt
      361 ggctcacagc ttgggttttc atggatgtgg aaaccatgta agccttcgct taatccttgt
      421 aaatcagggg taaacacaag accatagtta gattcagtaa tagtcactgt acccacatct
      481 ttgttaccgt ttactggatc aagttgttgc actttcactt caatagaagc acctttaggt
      541 atcgtattat gctcgtgtgc ttgcgcaaca ccaactgaac atactgcact aaccgctagt
      601 gctaataagg ttttcatatt cattgcaatc tcctttattg cagtaaaaac tcgcgactat
      661 tttagtcaat tttgttttaa aagccgataa atttttacga aaattttaca ttaattgtta
      721 tttgcagaaa attagaaccc gttatcacgt cttggataaa tgccaaaacc attgtgattc
      781 agagaattaa aaagaaaaat ttcggaatcc tcatcctttt ctgaaacctg attgcggtat
      841 ccatggcttt gtctttgtca tttaagtttg tgttcatgtt tttgaccttc tttgcttttt
      901 aaatcctttt taaattgccg acttcttccg ttccaaatgt cccatttgaa cattatcccg
      961 ccgcctaaaa tacgacccaa attcatcggt tgttcttgct gcgatacctg caaacaacag
     1021 ttcaatgggt ttattttgtt tattctaacc tattaccgtt taaataaatt tccaaaggct
     1081 tgggcaggcc gtaatctttc aaatgcgccg gctttatcca aatgccgtct gaagggcttt
     1141 cagacggcat ttgtccttca aacggcgtaa tcatcagcag ccggtgcgtc agacggtggg
     1201 tcagggcggt ttgttcgtcc atatctgcca tggtcaggga gaatttggcg gcaaagtcgg
     1261 aaagcccgtt caaactttca aaacacggca cgcaatacag cccgccccaa atgcctttgg
     1321 cggggcgttt ttccagcaaa atcgcgccgt cccggttgcg gacaatcagc cagtaaagcg
     1381 gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag ctcggcggtg cggttttgct
     1441 tttttgcttc gcagatgtcc gccatcgggc attggtggca caagggtttc gtccgtttgc
     1501 acacggtcgc gcccaaatcc atcaaacctt gtgtataggc aggcatatcg gcgttttcag
     1561 acggcagcag gctttcggca agtgtccaga gcgagttttc aaattttttg tcctgcggat
     1621 tgccgtcgcg ggcgaacacg cggcagagta cgcgtttgac gttgccgtcc aaaatggttt
     1681 cgcggcggtt gaaggagaag gcgcaaatgg cggcggcggt gcttctgcct acgccgcaga
     1741 gggtttccaa gtctttgcgc tccgacggaa acgtgccgcc gaattgcctg acgacttgtt
     1801 gcgcggcttt gtgcaggttg cgcgcgcggc tgtaatagcc caagcccgcc cacaacgaca
     1861 acacttcgtc ttgcggcgcg gcggcaagcg tctgaacggt cgggaatttt tctaagaagc
     1921 gcggatagta gtccaacacg gtggcgactt gcgtttgctg gagcatgatt tcggaaagcc
     1981 agacgcaata agggtttttg acctgccaag ggaggtggtg gcgaccgtgt tgtttttgcc
     2041 agcggatgag ccgttcggag aaggggatgg gtgtgttcat taatatcaat cggtggtttt
     2101 atttatattt aaaacggtat gttattgtat aacattgtga aaataattct tattgactta
     2161 ttttttgtag gggcatataa ctcatataaa gaaactttat tgcggtattg aaattattta
     2221 tcaacaagca aggagtatca gcatgaaagc aatggtttat cacggcgcaa acgacatccg
     2281 ttttgaagaa aaaccccgtc cgcagattat cgatccgacc gatgcggtgg tgaaaatcgt
     2341 caaaaccacg atttgcggta ccgatttggg tatttggaaa ggcaaaaaaa acccgaagtc
     2401 gccgacggcc gtattctcgg tcatgaaggc atcggtattg tagaagaagt cggcgaggct
     2461 gtaaaaaaca tcaaagtcgg cgataaagtc attatttcat gcgtcagcaa atgctgtact
     2521 tgcgacaact gcaaaatcca actttattca cactgccgta acggcggctg gattttgggc
     2581 tatatgattg acggtacgca ggccgaatac gtccgtaaca gcctcgtgcc gctgcccgac
     2641 aacgtcaacg aagaagtcgc cctgttgttg agcgacgcct tgccgaccgc ccaactgtac
     2701 agccctgtcg ccatcatcgt ttgcgatatg gacgaaaacc gtttgaaact ggcgaaagag
     2761 ttgggcgcga cccataccat cagccccgct tccggcgacg tctccaaaca agttttcgcc
     2821 atcgtcggcg aagacggcgt agattgtgcc atcgaagcgg tcggtatccc tgcaacttgg
     2881 aatatgtgcc aagacatcgt gaaacccgac ggccatatcg ccgtcgtcgg cgtacacgtt
     2941 caatccgttg atttcaaact ggaaaaactg tggattaaaa atcttgccat cactaccggc
     3001 ctggtaaacg ccaataccac cgaaatgctg atgaaggcaa tttccagcag ctccgtcgat
     3061 tacaccaaaa tgttgaccca ccgtttcaaa ttcagcgaat tggaaaaagc ctacgacgtg
     3121 ttcaaacacg ccgccgaaaa tcaagcgatg aaagtggttt tggaagcgga ttaatcggcg
     3181 ttgaatcagt tgttttttaa gcggatgccg tctgaatgcg tttcagacgg cattttttca
     3241 gctgcgtaaa acgggctgtc cggctgctgt cgaatgaact gaacgttatt tttattatgc
     3301 ggaaggcgga tgggcatttt tacgatgcgg ttcaggcttt gggttttaac gccgccgctt
     3361 ctttggcgag gcgggtgatg gtgtcccagt ctttgttttt cacagcttct ttcggggtca
     3421 gccaagagcc gccgacgcac aagacgttgg gcagtgccag gtaatcgggt gcggtggcga
     3481 ggctgatgcc gccggtcggg cagaagcgca cgtcggcata aggaccgtaa agggctttga
     3541 gcatggcttt gccgccgacg acttcggcgg ggaaaagttt gagggtgtcg atgccgtgtt
     3601 ccaaagccag ttggacttcg cccggagtgg caacgccggg aatcaggggg atgccgctgt
     3661 tgtggctggc tttggcgagg gattcgtgca aacccgggct gatggcgaaa accgcgcctg
     3721 cgtcttcgac ggctttgagc tgttcggggt tggtaaccgt acctgcgccg acgatggcgt
     3781 tgggtacttc tttggcaatc aggcggatgg cgtcgaggcc gacgggggtg cgcagggtaa
     3841 tttcgagggt ggggatgccg ccttcgacaa gggcgtggga caaatcgaca gcggtgctca
     3901 agtcgtcaat cgccatcaca ggcacaactg cgcctgcgct caggatttcg cggggggtca
     3961 gtttggacat ttcggttctc cgggtggaat ggggtatttt attaagatgg gacaggtcgt
     4021 aggtcggatt caagaaaccg acatcttgga cggctgtgtc ggattcgaga atccgaccta
     4081 cgtctatctt aaaacacttg aataattttt cagacggcct tcggtctgaa aaggccgtct
     4141 gaaacgcgca tcaggcaaat tcgccgccga aactcatggc accggtttcc gcgctgctgg
     4201 tcatgctgcg gaagttggcg aagagttcgc ggccgcagcc ttgttggttc gcgcccaagt
     4261 cgatgctttc gacttcgcgg gcattccatt cggtttcgtt aatcaggacg ttgagttcgc
     4321 cgctaacgga gtcgaagcgg atcaggtcgc cggtacggat tttggcgatg ttgccgccca
     4381 tcagggcttc gggtgtcatg tggatggagg ccggaacttt gccagatgcg ccggacatac
     4441 gaccgtcggt cagcagtgcc actttgaagc cgcggtcttg caggatgccc aaaggcgggg
     4501 tcagtttgtg caattcgggc ataccgttgg cacgcgggcc ttggtagcgg acgacgcaga
     4561 cgaaatcgcg ttccaactcg ccgcgttcaa atgccgccaa cacttcgcgt tggtcgttga
     4621 acacgatggc aggcgcttca ataatgcggc agccttcgcg cacggcggac actttaatca
     4681 cgccgcgtcc gatgttgcct ttcatcaggc gcagaccgcc gtcgggggag aacgggttgc
     4741 cggctttgcg caggatgtcg tcgttgccgc tggtttcggg ggcttcgcgc cattcgagtt
     4801 tgccgtcgat aaggaaaggc tctttggtgt agtggcgcat accgtgtccg acaacggtat
     4861 cgacatcgtc gtgcaacagg cctgcgtcca gcaattcgcg gataacgaaa ggtagaccgc
     4921 cggcagcggt aaagtggttt acgtcggctt tgccgttggg gtacacacgg atcagcaatg
     4981 ggatgatgga agaaatttcg tcaaagtcgt cccagttcaa aatcacgccg gcggcgcgtg
     5041 ccatggcaac gaggtgcatg gtgtggttgg ttgagccgcc ggttgccatc aggccaatca
     5101 aggcgttgat aaaggatttt tcggtcaaca tttcgcccaa aggtttaatc gtgccgtttt
     5161 tgatgccgcg cgcgaggtgt ccggcggcgt agcgggtcag cgcttcacgc aggtcggtgt
     5221 aagggtggac gaaagcggcg gcagggaggt gcacgcccat catttccatc atcatttggt
     5281 tggagtttgc cgtgccgtag aaagtg
//
LOCUS       DAWHRS010000139         5112 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWHRS010000139 DAWHRS010000000
VERSION     DAWHRS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.139"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            93..3926
                     /locus_tag="WIB82_001760"
     CDS             93..3926
                     /locus_tag="WIB82_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ3843026.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKTRWEWLLANLDTPLADYKARYGAA
                     VHAAPEAKNNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSNLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLIEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQIGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKNVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKYILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            4107..5108
                     /locus_tag="WIB82_001761"
     CDS             4107..5108
                     /locus_tag="WIB82_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ3843027.1"
                     /translation="MIDRQTNEPKQKNRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRERLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEAYEPPAHWEWIRKIRDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
BASE COUNT         1346 a         1498 c         1290 g          978 t
ORIGIN      
        1 aaaaattgaa ctacattatc gccttttcaa actcgcctga aaccgacttt tcagacggca
       61 ttcaaataaa aactgccaaa cacggacaca ccatgaccac gactaccgcc cctcagcgta
      121 ttcgggaaat cccctacaac tatacttcct acaccgaccg cgaaatcgtc atccgattat
      181 tgggcgacga agcgtggcaa atcctgcaag acctgcgcgg tcaacgcaaa accgggcgtt
      241 cggcgcggat gctgtttgaa gtgttgggtg atatttgggt ggtcgtgcgc aatccgtatc
      301 tggtcgatga cttgctggag cacccaaaac gccgcgccgc gctggtacgt gaaatgcgcc
      361 accgcttaaa tgaaatccgc aaacgccgcg acgataatcg gcaagtggat gtgttggttg
      421 ccgcagcaga aaaagcagtc gagcgttttg atagcagttt tgatgaaacc agccaaaaac
      481 ggcggcagat tttggagcgt ttgagcaaaa tcaccaagcc gcacaatatt atgttcgacg
      541 ggctggcgcg ggtaacgcac gttaccgatg caaccgactg gcgcgtggag tatccgtttg
      601 tcgtcgtcaa tcccgacacg gaggctgaaa tcgcgccttt ggtgcgcgcc ttaatcgagc
      661 tggatttggt cattattccg cgcggcggcg gcacgggtta taccggcggc gcgattcctt
      721 tggacgcaaa cagcgcagtc atcaataccg aaaaactcga caagcatcgt ggtgttgaat
      781 acgttgagct ggcaggcttg gacggcaagc atccgattat ccggtgcggc gcgggcgtgg
      841 ttacgcggcg ggtggaagaa accgcgcatc aggcaggttt ggtgttcgcc gtcgatccga
      901 cttctgccga cgcgtcatgc gtgggcggta atgtggcgat gaacgcgggc ggcaaaaaag
      961 ccgtgctgtg ggggacggcg ttggacaacc tcgcctactg gaacatggtt aaccctcaag
     1021 gcgaatggct gcgtatcgag cgcgtgcgcc acaatttcgg caaaatccac gacgaagaaa
     1081 ccgccgtgtt cgacgttcac acgctggatt cagacggcat caatatcgtt aaaaccgaac
     1141 gcttggaaat ccccggccac aaattccgca aagtcggttt gggcaaagac gttaccgaca
     1201 aattcttgag cggcctgccc ggcgtgcaaa aagaaggtac agacggcatc atcaccagcg
     1261 ttgccttcgt gttgcataaa atgccgaaat acacgcgcac cgtgtgtatg gagtttttcg
     1321 gtacggtcgc caccgccacg ccatctattg tcgaaatccg cgactttttg cttgcccatg
     1381 aaagcgtgcg gctggcgggt ttggaacatt tggactggcg ttatgtccgc gccgtcggct
     1441 acgccaccaa agcggcgggc aagggacgac cgaaaatggt tttgctggca gacgtggttt
     1501 cagacgacga agccgccgta gaggcagccg ccgaacacat ctgtgaactc gcacgcgccc
     1561 gcgacggcga aggctttatc gccgtatcgc ccgaagcccg caaaaccttc tggctcgacc
     1621 gcagccgcac cgccgccatc gccaaacata ccaacgcctt taaaatcaac gaagacgtgg
     1681 tcatcccgct cgaaaggctc ggcgagtatt cggacggcat cgaacgcatc aacattgagc
     1741 tttccatcca aaacaagctc aaactctgtg ccgccttgga gcaatatctt tcgggcaaac
     1801 tccccatcga caaaatgggc actgacctgc cgaccgccga actgttgggc gaacgcggca
     1861 aacacgccct ggcccacgtt tccgccgtca aaacgcgttg ggaatggctg ctcgccaatc
     1921 ttgacacgcc gcttgccgac tacaaagccc gctacggcgc agccgtccac gccgcacccg
     1981 aagccaaaaa caatgaaagc tgctttattg ccttccgcga tttccgcctg cgcgtgtctg
     2041 tcaaagcgga cgtaatgaaa ccgctttctg aaatcttcag cggcaaaacc gacaccaaaa
     2101 ttatccaagg cttgggaaaa atccacgcaa aaaccgtacg cagccgcgtc tttgtcgccc
     2161 tgcatatgca cgccggcgac ggtaacgttc acaccaatat tccggttaac tcagacgatg
     2221 ccgaaatgct tcagacggca taccgctcag tcgaacgcat tatgaaaatc gcccgttcgc
     2281 ttaacggcgt gatttccggc gaacacggca tcggcattac caagctcgaa tttttaagcg
     2341 acgaagaaat gcagccgttt tgggactaca aaaaccaagt cgatccgaaa cacaccttca
     2401 accgtcacaa actgatgaaa ggctcggact tacgcaacgc ctacacgccg tccttcgagc
     2461 tgttgggcgc ggaatcgctg attatggaaa aatcaaacct cggcacgatt gccgattccg
     2521 tcaaagactg cctgcgctgc ggcaaatgca aacccgtctg ctctactcac gttccgcgtg
     2581 ccaacctgct gtacagcccc cgcaacaaaa tcctcggcgt gggcttattg atcgaagcct
     2641 tcttatacga agaacaaacc cgccgcggcg tttccatcaa acactttgaa gaactcatgg
     2701 acatcggcga ccactgcacc gtgtgccacc gctgcgtcaa accctgcccc gtcaacatcg
     2761 acttcggcga cgttaccgta gccgtccgca actatcttgc cgattccggc cacaaacgat
     2821 ttgcgcctgc cgcagctatg ggtatggcgt ttttgaacgc caccggcccg aaaaccatca
     2881 aagcccttcg cgccgccatg atacagatcg gcttcccagc gcagaatttc gcctacaaaa
     2941 tcggcaaact tcttccaatc ggcacgaaaa agcaaaaagc cgaacccaag gcaaccgtcg
     3001 gcaaagcccc gattaaagaa caggttatcc atttcatcaa ccgcccactg cccaaaaacg
     3061 tacccgccaa aacaccgcgc tccttattgg gcatcgaaga cggcaaaagc atccccatca
     3121 tccgcaaccc cgccgcgccc gaagatgccg aagccgtgtt ctacttcccg ggttgcggct
     3181 ctgagcgtct gttcagccaa atcggacttg ccgttcaagc catgctctgg cacgtcggcg
     3241 tacaaaccgt cctgccgccc ggctatatgt gttgcggcta tccgcaagac gcaggcggca
     3301 ataaggcaaa agccgaagaa atgagcacca acaaccgcgt ggctttccac cgtatggcga
     3361 acaccctcaa ctacctcgac atcaaaaccg tcgtcgtcag ttgcggcact tgttacgacc
     3421 agctcgaaaa ataccgcttt gaagaaatct tccccggctg ccgaatcatc gacatccacg
     3481 aatacctgct cgaaaaaggc gtgaaactcg acggcgtaaa aggtcagcaa tacctctacc
     3541 acgacccctg ccataccccc atcaaaacca tgaacgccac ccaaatggcc agcagcctga
     3601 tggggcagaa agtcgtttta agcgaccgct gctgcggcga atccggtatg tttgccgtca
     3661 aacggccaga catcgccact caggtcaagt tccgcaaaca agaggaaatc gagaaaaacc
     3721 tcaaagagct gccgcagggc gaacccgtca aaatgctgac ctcctgcccc gcctgcctgc
     3781 aaggcttgag ccgctacgcc gacgacaaca atatgcctgc cgactacatc gtcatcgaaa
     3841 tggcgaaata catcctcggc gaaaactggc tggatgagtt tgtaaaaaaa gccaacaacg
     3901 gcggtgtaga gaaagtgttg ctgtaacaac ggacacggaa atgccgtctg aacgccgaaa
     3961 gccttcagac ggcattgttt gaaccaaata tagtggatta acaaaaatca gaacaaggcg
     4021 acgaagccgc agacagtaca aatagtacgg caaggcgagg caacgctgta ctggtttaaa
     4081 tttaatccac tatacctacc caaatcatga ttgacagaca aacaaacgaa cccaaacaaa
     4141 aaaaccgaat catccttgcc cctatgcagg gtctggtcga tgacgtgatg cgcgacctgc
     4201 tgacgcgtat tggcggctac gacgaatgcg tcagcgaatt tgtacgcatt acccataccg
     4261 tgcattcccg atccatatgg ttaaaatatg tccccgaaat cgccaacgga aacaaaacgt
     4321 tttccggcac gccctgcacc gtccaacttt tgggcagcga tgcggacaat atggcggcga
     4381 atgcgctgga agccgtccgc ttcggtgcga acaaaatcga tttgaacttc ggctgccccg
     4441 cgcccaccgt caacaaacac aaaggcggcg caatcctttt aaaagagccg gaactgatat
     4501 tccacatcgt caaaacgctg cgcgaacgtt tgcccgcaca tattccgctc accgcaaaaa
     4561 tgcggctcgg ttacgaagac aaaagccggg ctttggaatg cgcctgtgcg attgccgaag
     4621 ggggcgcatg cggactgacc gtacacgcgc gtaccaaagc cgaggcttac gaaccgccgg
     4681 cgcattggga atggatacgc aaaatccgcg ataccgtcaa tattcccgtt accgccaacg
     4741 gcgacgtttt cagcctgcag gactatatcg gcatcaaaac aatcagcggc tgcaacagcg
     4801 tgatgctcgg tcgcggcgcg gtcatccgcc ccgatttggc gcggcaaatc aagcaatacg
     4861 agaacggcgg gccggtcaaa gacacggatt ttgccgaagt ttccaaatgg atacggcagt
     4921 ttttcgagct gtgcctgaca aaagaggcaa acaacaaata tccgctggcg cggctgaaac
     4981 agtggctggg tatgatgaag aaagaatttg cagcagcaca aaatctgttc gaccgcgtcc
     5041 gaacggttaa ggatgcggac gaagttcgga acatcttggc tgaatttgag cgagaaatga
     5101 atacttgaat at
//
LOCUS       DAWHRS010000140         5037 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWHRS010000140 DAWHRS010000000
VERSION     DAWHRS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.140"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(190..2484)
                     /locus_tag="WIB82_001762"
     CDS             complement(190..2484)
                     /locus_tag="WIB82_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3843028.1"
                     /translation="MRREAKMEQTTLKPIVLSILLTNTPLLAQAHETEQSVDLETVSV
                     VGKSRPRATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPV
                     IRGQTGRRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAG
                     LVDVADGKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKS
                     GDYAVPRYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYD
                     DCHADIIWQKSLINKRYLQLYPHLLTEEDVDYDNPGLSCGFHDDDDAHAHAHNGKPWI
                     DLRNKRYELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNQTQNARIELR
                     HQPIGRLKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTL
                     EGGVRVEKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLS
                     LTASHQERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLA
                     LYRNRFGNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYR
                     IGVSGDYVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRI
                     DANLDYYRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVY
                     AHSSFLSDTPQMGRSFTGGVNVKF"
     gene            2888..3478
                     /locus_tag="WIB82_001763"
     CDS             2888..3478
                     /locus_tag="WIB82_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ3843029.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFRGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            3542..4600
                     /gene="trpD"
                     /locus_tag="WIB82_001764"
     CDS             3542..4600
                     /gene="trpD"
                     /locus_tag="WIB82_001764"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ3843030.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(4752..4967)
                     /locus_tag="WIB82_001765"
                     /pseudo
     CDS             complement(4752..4967)
                     /locus_tag="WIB82_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1149 a         1154 c         1429 g         1305 t
ORIGIN      
        1 ttctgctttt gatttttctg cttttgcgag aatgacggcg tgaaagcaag aatgatgaaa
       61 caaagaaaat gggaatgatg gcatagtggt ttgttctttg tctttgccat atttcctaac
      121 aaattgatta aaaagaaaaa aggttttcag aatgccgtct gaaaaccttt tttgtttgcc
      181 tgtccgattt taaaacttca cgttcacgcc gccggtaaag ctgcggccca tttgcggcgt
      241 atcagagaga aagctgctgt gggcgtaaac ggattggttg agcaggttgt cggctttgac
      301 gtaccaattc cactcgccat agcgcgtatt gcggcggtag tttgcgccga ggttgagcat
      361 atggtgtccg ggcgtgcgtg tttcgtagcg ggcgagtttg ttttgggcga acacgcggta
      421 gtagtctaaa ttggcatcga tgcggtcggt cagcgaggct ttcaggtgga cgccgaggcg
      481 cgcagccgga acgcgagggg cattttggtc gtcctgtgcg atgaaaggac ggttgccgta
      541 ggcatcttcc ctgccgggta gggacggcag gtttttcaga cggcctcgta catagtcgcc
      601 ggaaacgccg atgcggtagc gcggtgtcgg tttgaagtag atttcgcctt ctgcgccgta
      661 gaagtccgca ccggattggt tgtagcgcac gagcttcatt tcgctgtcgt cttcgatgga
      721 tttggggccg cgtccgtcgt ttaaggtttg ggcgtaaatg tagttgccga agcggttgcg
      781 gtagagtgcc agattgtatt gccagcggtc gccttcgtag cccagcgcga gttcgatatt
      841 gttggaacgc tctttgttga ggtgtttgtt gccgacttca aaggtgttgg tggcgacgtg
      901 tttgccgtgt gcgtacagct cttgcgttga cggcaggcgt tcctgatggg aggcggtcag
      961 gctgagtttg tgttgtggcg tgaaatacca gttgcccgaa agtgcgaatg agcgggcggt
     1021 ttggcggtgc gcgccgaggt cgggcagggg atggttgtag tagttttccc gatcaatcaa
     1081 tgctttgtcg tagcggatgg aggctttttg tttttccacg cgtacgccgc cttcaagcgt
     1141 gaagttgtcc cagtttgcct gttctacacc gaaaaagctg taatgttgca ctttattgtc
     1201 aagcagcatc ggttgtttga ccgcttcgga tgtggcagat aaagcactgg atttttgttg
     1261 taaatattgc acgccccagc tgcctttcag acggcctatg ggttggtggc gcaactcgat
     1321 gcgggcgttt tgcgtttggt tgttaaaaaa gttttcgact gcatcgcctg ctttttcgtc
     1381 gtggtggtag tcgttgcggt tcaggtgtac gcgcagggct tcaaaaccgg ggaatggctg
     1441 cttccattcg gcgcggagtt cgtagcgttt gttgcgcagg tctatccaag gtttgccgtt
     1501 gtgggcatgg gcgtgtgcat catcgtcgtc gtggaagccg cagctcaagc ccggattgtc
     1561 gtaatcgacg tcttcttcgg tcaacaggtg cggataaagc tgtaaatagc gtttgttaat
     1621 caagctcttt tgccagatga tgtcggcgtg gcaatcatcg tattcgtggc tgtgggcagg
     1681 cagaccatat tggtcgcgac ggtcgctgta cgctacgccg ataaaacctt tttcgccaac
     1741 ccaagacagc ccgatgctgc ccgtttgcga atcggcgtgg ctgtcgggta ggcgtttcag
     1801 attgcggtaa cgcggtacgg cgtaatcgcc cgatttgcgg tacaagcctt cggtatgcaa
     1861 tacaaagttt ttgcccaagc cgatattgat gccgccggac gtgagttttt ccagattgcc
     1921 gctgctcaaa cgcagcccta gttcgcccga tacgccgttt tcaggcattt tttcggggat
     1981 tttgccatcg gcaacatcca ccagccccgc cacattgccc gagctgtaca agagcgtaac
     2041 cggcccgcgc aggatttcga cctgttgcga caaggcggta tctaccataa tggcgtgatc
     2101 gggcgaaaaa tccgccatat cgcctgtttc gccgtgatgg ttcaacactt taatccgcct
     2161 gcctgtttga ccgcgaataa cgggagcgga cgcgccgccg ccgtattgcg aggcgtgaat
     2221 gcccggcacg ccgtccaaag catcgcccaa gttgacggct ttttgtcgca aggtgtcgcc
     2281 gctgatgatt ttgtcggagg cggtcgaagt gtgcagcagc ccggatgtgg cgcgcggacg
     2341 gcttttgccg acgacgctga ccgtttccaa atccaccgat tgctcagttt catgcgcttg
     2401 ggcgaggagg ggtgtgttgg ttaaaagaat tgataaaaca atgggcttga gagtggtttg
     2461 ttccattttg gcttctcgtc gcatttcaaa agtttgttat tatataacat tacatttttt
     2521 atatcataag attttgagaa cactcagagg gcataggcaa aagtttttca aatgaaacgg
     2581 ttgcggcatc gggcggtgtc catttgtatc cgccgtcctt cgggggcgcg gttgatgttg
     2641 acgcagattc cgctgtgttt gcccattatg ttcggctgcg ggataaccaa aatttatgag
     2701 tgcataaaaa cggcacgttc ccgaacggtc gaaaaggtgg caaaatggcg tacttgtcag
     2761 aacgcgaggc tctgcgccag gttcacgagg gcgcatcggg cacgctaaga tataagaggg
     2821 tatggatggg ggtatcggaa atgcagttaa taacaaacaa attataaatc aataggttaa
     2881 tcacaaaatg cttttgttta tcgacaatta cgacagtttt acttacaaca tcgtccagta
     2941 tttcgcagaa ttggggcagg aagttgccgt gcgccgcaac gatgatatta cgttggagga
     3001 aatcgaggca ttgaatccgc aatatctcgt tatcggcccc ggtccgtgtt cgcccaaaga
     3061 agcggggatt tccgtggcgg cgatgcgcca tttcgccggc cggctgccga ttatgggcgt
     3121 gtgcctcggg catcagacga taggcgaggc gttcggcggc aggatagtcc gcgccaaaac
     3181 gctgatgcac ggtaaggtgt cgcccgtgtc ccattcgggc aagggtatgt ttagggggtt
     3241 gcccaatccg gttacctgta cgcgttatca cagcctcgtt atcgatcgga atacgatgcc
     3301 cgaatgtttg gaagtaacgg cttggactga ggacggcgag attatgggtg tgcgccataa
     3361 ggaatatgcc gtcgagggcg tgcagttcca ccccgaagcc ctcttgaccg aacacggaca
     3421 tgatatgtta aacaattttt tagttgaatt tcaaaacttc aaaccgcaaa aaatctgacg
     3481 tgatgccgtc tgaagccctt cagacggcat tttcgtccga atattgaacg gaggacaaaa
     3541 aatgattaca ccgcaacagg ccatcgaacg attaatcagc aataacgagt tgttttacga
     3601 tgaaatgacc gacttgatgc gtcagattat gagaggacag gttctgccgg agcagatagc
     3661 ggccattttg acaggattgc gtatcaaggt tgaaaccgtt tccgaaatta ccgcagctgc
     3721 agccgtcatg cgcgagtttg cgacaaaagt gccgctggag aatgcagagg ggctggtcga
     3781 tatcgtcggt acgggcgggg atggcgcgaa aaccttcaat atttcgacga cttcgatgtt
     3841 tgttgccgca gcggcaggcg cgaaggttgc caaacacgga ggccggtcgg tctcttcctc
     3901 cagcggtgcg gctgacgtgg tggagcagat gggcgcaaac ctcaacctga ctcccgaaca
     3961 ggttgcccaa agtatcaggc agaccggcat cgggtttatg ttcgcgccca atcaccacag
     4021 tgccatgcgc catgtcgccc ctgtacgccg ttcgctcggt ttccgaagta ttttcaacat
     4081 attgggtccg ttaacgaatc ctgcgggcgc gccgaaccag cttttgggcg tgttccacac
     4141 cgatttgtgc ggcattttgt cgcgggtctt gcaacaactt ggttcaaaac acgttttggt
     4201 tgtttgcggg gagggcggtt tggatgaaat tacactgacg ggcaaaacac gcgttgccga
     4261 gctcaaagac ggaaaaatca gcgaatacga catccgccca gaagatttcg gtatcgaaac
     4321 ccgccgcaat ttggatgaaa tcaaagttgc caatactcag gaatctttgt tgaaaatgaa
     4381 tgaggtgctg gaaggaagag aaggggctgc gcgcgatatc gtattgctca acaccgccgc
     4441 cgccctgtat gccggaaatg tcgctgcttc gctttcagac ggcatatctg ccgcacggga
     4501 agccatcgat tcaggcaggg ccaaatcgaa aaaagaggag tttgtcggtt ttcaaccaca
     4561 acaaagatgc cattttcttg gaaagatgga gcttgggtga tgccgccatg attatggaac
     4621 ttttgtggca aaacataagc acttcacgaa gagaacttac caaactgttt ttatataaaa
     4681 acttggggct gtactagata accagaccaa attcccatta actaattgtc ttaaaatctg
     4741 aatttgagat tctatttaaa atgccattgg catttcttta aatgcagccc caaatgctct
     4801 ttgggaatgc cgttaaactt acgtaaatgg ctaaattcac taatatcaag cacatcataa
     4861 ctacgaaagg tatccgtgta tacaatgcca tcaggcttaa ctttctttcg gataattggc
     4921 aacaatgtcg ctgattgcgc attaggaaca acgacggtat aaaccttata tattgcgtcc
     4981 ctaagaaggg acgattaaca aaaattaacg tcctttactt tctacaagta acagggc
//
LOCUS       DAWHRS010000141         4992 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWHRS010000141 DAWHRS010000000
VERSION     DAWHRS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.141"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1444)
                     /gene="tldD"
                     /locus_tag="WIB82_001766"
     CDS             complement(<1..1444)
                     /gene="tldD"
                     /locus_tag="WIB82_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ3843031.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTL"
     regulatory      1622..1725
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1792..3015
                     /gene="cytX"
                     /locus_tag="WIB82_001767"
     CDS             1792..3015
                     /gene="cytX"
                     /locus_tag="WIB82_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ3843032.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIESFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            3012..4112
                     /locus_tag="WIB82_001768"
     CDS             3012..4112
                     /locus_tag="WIB82_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ3843033.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIVLFDKGCRRGENAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQTQYDWLIDCRGYGAKTAWNQAPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            4134..4751
                     /gene="thiE"
                     /locus_tag="WIB82_001769"
     CDS             4134..4751
                     /gene="thiE"
                     /locus_tag="WIB82_001769"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ3843034.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDE
                     LKREIARCVAACQGSRTQLFINDHWHEAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELNRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          983 a         1520 c         1425 g         1064 t
ORIGIN      
        1 gcagggtcgg ttgccccacg ccgacgggga cgctctgccc ttctttgccg cacacgccga
       61 caccgctgtc gagcgcggta tcgttgccta tcatcgaaac gtgtttcagc acttcgggac
      121 cgttgccgat aatggtcgcg cctttgacgg gatattgcag cctgccgcct tccacccacc
      181 atgcttcgga cgcgctgaac acgaatttgc cgctggtaat gtccacctgt ccgccgccga
      241 agttgacggc gtaaatgcct ttgtcgatgg acgcgatgat ttcttccggc tcatagctgc
      301 cgttttccat aaaggtattg gtcatgcgcg gcataggggc ggaagcgtaa ctttcgcggc
      361 ggccgttgcc ggtggattgc gtacccgtca ggcgggcatt ggtttcgtcc tgcatatagc
      421 cgactaaaat gccgtcttca atcaatacgg tgcggcgggt ttcgttgcct tcgtcgtcga
      481 tgttgagcga accgcgccgg ccggcaatat cgccctgatc gacgacggta acgcctttgg
      541 cggcgacgcg ctcgcctatt ctgccggaaa agacgctggt ccccttgcgg ttgaaatcgc
      601 cttccaaacc gtgtccgacc gcttcgtgca gcaacacgcc cggccagccg ttgcccaaaa
      661 cgacggtcat ttcgccggcg ggcgcggggc gggattcgag gttggtgagt gcctgtttga
      721 cggcggcatc gacaaactgc cgcaccaagt tttcatcgaa ataagccaag tcgtagcgtc
      781 cgccgccgcc cgcgctgccc tgttcgcggc gttcgccctg tttggcgatg acggtaacgt
      841 tcaggcgcac catcgggcgg atgtcggcgg cgtgtttgcc gtccagacgg gcgaggtaaa
      901 ccatatcgta ttcgcaggtc aaaccggcca tcacttgcac gatgcgcgga tcggcggctt
      961 tggcgattgc ttccactttg ttcaacagcg cgactttggc ggcggaatcg aggccggcaa
     1021 tggggtcgga cgcggaacaa accggcttgc cgcgcgtttc agacggcatt ttggcggaca
     1081 ccttgccgcc tgccgcccca atcgcgcgga cggcgcgggc ggaacggttt atcgaatcga
     1141 tgcacaggct gtcggcgtag gcaaaggcgg ttttgtcgcc cgaaacggcg cgcacgccca
     1201 cgccctgatc gatttggaag ctgcccgatt tgacgatgcc ctcttccaaa tgccagcttt
     1261 cataggcggt gcgctggcag tagatgtcgg cgtaatcgac gtggtgcgcg ccgatgatgc
     1321 acaggctttt ggcgagcagt tcgggggaaa ggcggttggc ttcgagcagc cgcgcctgta
     1381 cggcggaata ggtcggatgc atagtgtcgg cgcataaaaa atcaggggct tgattatacg
     1441 gcatttgtta tatagtggat taacaaaaaa cagtacggtg ttgcctcgcc ttgccgtact
     1501 atttgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatagaaat
     1561 gcgccgtgcc gccgaaaatg taagaatttt gccaacgcac gttgctttgt gtaaacttaa
     1621 agctttcttg tcggagtgcc gccgccgggc ggctgagatt gcgaaagcag aatccgtaga
     1681 acctgtcggg gtaatgcctg cgtaggaaac aaaccgtcaa atgccttatc aggcttccgt
     1741 tcccttttcc gcacttcccc gcccaccttc cttcttttta aaggacgtca tatgtcgggc
     1801 aatgcctcct ctccttcatc ttccgccgcc atcgggctga tttggttcgg cgcggcggta
     1861 tcgattgccg aaatcagcac gggtacgctg cttgcgcctt tgggctggca acgcggtctg
     1921 gcggctctac ttttgggtca tgccgtcggc ggcgcgctgt tttttgcggc ggcgtatatc
     1981 ggcgcactga ccggacgcag ctcgatggaa agcgtgcgcc tgtcgttcgg caaacgcggt
     2041 tcagtgctgt tttccgtggc gaatatgctg caactggccg gctggacggc ggtgatgatt
     2101 tacgccggcg caacggtcag ctccgctttg ggcaaagtgt tgtgggacgg cgaatctttt
     2161 gtctggtggg cattggcaaa cggcgcgctg attgtgctgt ggctggtttt cggcgcacgc
     2221 aaaacaggcg ggctgaaaac cgtttcgatg ctgctgatgc tgttggccgt tctgtggctg
     2281 agtgccgaag tcttttccac ggcaggcagc accgccgcac aggtttcaga cggcatgagt
     2341 ttcggaacgg cagtcgagct gtccgccgtg atgccgcttt cctggctgcc gctggccgcc
     2401 gactacacgc gccaagcacg ccgcccgttt gcggcaaccc tgacggcaac gctcgcctac
     2461 acgctgaccg gctgctggat gtatgccttg ggtttggcag cggcgttgtt caccggagaa
     2521 accgacgtgg caaaaatcct gctgggcgca ggtttgggtg cggcaggcat tttggcggtc
     2581 gtcctctcca ccgttaccac cacttttctc gatgcctatt ccgccggcgc gagtgcgaac
     2641 aacatttccg cgcgttttgc ggaaacaccc gtcgctgtcg gcgttaccct gatcggcacg
     2701 gtgcttgccg tcatgctgcc cgttaccgaa tatgaaaact tcctgctgct tatcggctcg
     2761 gtatttgcgc cgatggcggc ggttttgatt gccgactttt tcgtcttgaa acggcgtgag
     2821 gagattgaaa gctttgactt tgccggactg gttctatggc tggcgggctt catcctctac
     2881 cgcttcctgc tctcgtccgg ctgggaaagc agcatcggtc tgaccgcccc cgtaatgtct
     2941 gccgttgcca ttgccaccgt atcggtacgc cttttcttta aaaaaaccca atctttacaa
     3001 aggaacccgt catgacccgt atcgccatcc tcggcggcgg cctctcggga aggctgaccg
     3061 cactgcagct tgcagaacaa ggttatcaga ttgtactttt cgataaaggc tgccgccggg
     3121 gcgaaaacgc cgccgcttat gttgccgccg cgatgctcgc gcctgcggcg gaagcggtcg
     3181 aagccacgcc tgaagtcatc aggctgggca ggcagagcat tccgctttgg cgcggcatcc
     3241 gatgccgtct gaacacgcac acgatgatgc aggaaaacgg cagcctgatt gtgtggcacg
     3301 ggcaggacaa gccattatcc agcgagttcg tccgccatct caaacgcggc ggcgtggcgg
     3361 atgacgaaat cgtccgttgg cgcgcagatg aaatcgccga acgcgaaccg caactcggcg
     3421 gacgtttttc agacggcatc tacctgccga ccgaaggcca gctcgacggg cggcaaatat
     3481 tgtctgcact tgccgacgct ttggacgaac tgaacgtccc ttgccattgg gaacacgaat
     3541 gtgcccccga agacctgcaa acccaatacg actggctgat cgactgccgc ggctacggcg
     3601 caaaaaccgc ttggaaccaa gcccccgagc acaccagcac cttgcgcggc atacgcggcg
     3661 aagtggcgcg ggtttacaca cccgaaatca cgctcaaccg ccccgtgcgt ctgctccatc
     3721 cgcgttatcc gctctacatc gccccgaaag aaaaccacgt cttcgtcatc ggcgcgaccc
     3781 aaatcgaaag cgaaagccaa gcccccgcca gcgtgcgttc agggttggaa ctcttgtccg
     3841 cactctatgc cgtccacccc gccttcggcg aagccgacat cctcgaaatc gccaccggcc
     3901 tgcgccccac gctcaaccac cacaaccccg aaatccgtta caaccgcgcc cgacgcctga
     3961 ttgaaatcaa cggccttttc cgccacggtt tcatgatctc ccccgccgta accgccgccg
     4021 ccgtcagatt ggcagtggca ctgtttgacg gaaaagacgc gcccgaacgc gatgaagaaa
     4081 gcggtttggc gtatatccga agacaagatt aaagccgccc gaaaggacac cttatgacct
     4141 tcccgcccct aaaatccccg ctcaaattct acgccgtcgt ccccaccgcc gattgggtgg
     4201 ggcgcatggt caaagcaggt gccgacacgg tgcaactgcg ctgcaagacc ctgcacggcg
     4261 atgaattgaa acgcgaaatc gcccgctgcg tcgccgcctg tcaaggcagc cgcacacagc
     4321 ttttcatcaa cgaccactgg cacgaggcaa tcgaagcggg cgcatacggc gtacatctcg
     4381 ggcaggaaga catggacacc gccgaccttg ccgccatcgc cgccgccggt ttgcgtttag
     4441 gtttgagtac gcactccgtc gccgaactca accgcgccct gtccgtacat cccagctacg
     4501 tcgccagcgg cgcgattttc ccgaccacga ccaaacaaat gcccaccgcc ccgcaaggct
     4561 tggacaaact gtgcgagtat gtgaaacagg caggcggcac gcccgtcgtc gccatcggcg
     4621 gtatcgactt gaacaacgcc cgagccgtac tcgccaccgg cgtttcctca ctcgccgccg
     4681 tccgcgccgt aaccgaagcg gcaaatcccg aagcggtggt taaagcgttt caggctttgt
     4741 gggatggata aaaccgaaag aagaaaattc aattgccgtg taggcaaaac ttagcccgtt
     4801 atcgcaaaca tacttaactt taaatgtggc atatcatcaa attccgtcat tcccgcgcaa
     4861 gcgggaatcc gccttaaaac ttgagaaacc atcatttgaa aaacagtttc cgaatttcaa
     4921 aaatggattc ccgcctgcgt gggaatgacg gcaaccggcc ggttgcgtat caaaaaataa
     4981 agtaattcgg ct
//
LOCUS       DAWHRS010000142         4871 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWHRS010000142 DAWHRS010000000
VERSION     DAWHRS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.142"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            86..1561
                     /locus_tag="WIB82_001770"
     CDS             86..1561
                     /locus_tag="WIB82_001770"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ3843035.1"
                     /translation="MISKQEYQAQAAQDYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSNPESEWQETQNKARAVIHAAQMVQEGLDK"
     gene            1558..2103
                     /locus_tag="WIB82_001771"
     CDS             1558..2103
                     /locus_tag="WIB82_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ3843036.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKNREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            2100..3236
                     /locus_tag="WIB82_001772"
     CDS             2100..3236
                     /locus_tag="WIB82_001772"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ3843037.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGKPDWFPLQS
                     RPDARLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            3283..3765
                     /locus_tag="WIB82_001773"
     CDS             3283..3765
                     /locus_tag="WIB82_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3843038.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            3871..>4871
                     /locus_tag="WIB82_001774"
     CDS             3871..>4871
                     /locus_tag="WIB82_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3843039.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALLDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISTESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNI"
BASE COUNT         1381 a         1483 c         1046 g          961 t
ORIGIN      
        1 cggttagact gtcaacaatt ccgataaaat gctatactcg cgcccattgc cgcgccgtcc
       61 gaaaccgccc tttgggaaag cagaaatgat cagcaaacaa gaataccaag cccaagccgc
      121 ccaagactac aaccgcatcc cgctcgtgca agaacttctt gccgacttgg atacgccgct
      181 ttccctctat ctcaaactcg ccaaccgccc ctatacctac ctgctcgaat ccgttgtcgg
      241 tggcgaacgt ttcggccgct attcctttat cggcctgcct tgcagccact atctcaaagc
      301 cggcggcaaa cacgtcgatg tttatcaaaa cggcgaaatt gtcgaacaac acgacggcaa
      361 tccgctgccc tttatcgaag ccttccacaa ccgcttcaaa acacccgaaa tcccaagcct
      421 gccgcgcttt accggcggac tggtcggcta cttcggttac gaaaccatct acaattttga
      481 acacttcgcc caccgcctga aaaacaccac caaagccgac ccgctcggca cgcccgacat
      541 cttgctgatg ctgtcgcaag aattggcggt gatcgacaat ttgagcggca aaatccatct
      601 cgtcgtttac gccgatccgt cgcagcccga cggctacgaa cgcgcccgcg aacgcctcga
      661 agacatccgc acccagttgc gccaaagctg cgccatcccg ctctcgctcg gcagcaaaca
      721 caccgaagcc gtcagcgaat tcggcgaaga gccgttcaaa gcctgcgtta acaaaatcaa
      781 agactacatc ttcgcaggcg actgcatgca ggttgtcccc agccagcgca tgagcatgga
      841 attcaccgac agcccgctcg ccctctaccg cgccttgcgc acgcttaacc cttcgcctta
      901 tctcttttac tacgatttcg gcgatttcca catcgtcggc tcctcgcccg aaatccttgt
      961 ccgccgcgaa cgcgacgatg tcatcgtccg ccccatcgcc ggcacgcgcc tgcgcggcaa
     1021 aacccccgcc gaagaccttg ccaacgaaca agatttgtta agcgatgcca aagaaatcgc
     1081 cgaacacgtc atgttgattg acttaggacg caacgacgtc ggccgcatca gtaaaaccgg
     1141 cgaagtcaaa gttaccgaca aaatggtgat tgaaaaatac tcccatgtaa tgcacatcgt
     1201 ttccaacgtc gaaggccgtc tgaaagacgg catgaccaac atggacatcc tcgccgccac
     1261 gttccctgcc ggcacacttt ccggcgcacc caaagtccgc gccatggaaa tcatcgaaga
     1321 agtcgaacca agcaaacgcg gcatctacgg cggcgccgtc ggcgtatggg gtttcaacaa
     1381 cgacatggat ttggcaatcg ccatccgcac cgccgtagta aaaaacaaca cgctatacgt
     1441 ccaaagcggc gcaggcgtag ttgccgattc caatcctgaa tcagaatggc aggaaacgca
     1501 gaacaaagcc cgtgccgtca tacacgccgc gcaaatggta caggaagggt tggataaatg
     1561 acttatgaaa aactttatga gcttcgccaa acactcagac cgactaccaa agacggtctc
     1621 tatacagata atgaaaaaaa tcgagaaatc ctaacggtta gatggagcgg caacacagaa
     1681 aatcctaaaa atttctcagc tgtcgctata ggaattaatc caagtaaagc aaatgatgaa
     1741 agaagcgaca aaacccttac ccaacttgca cgtttcttgg atatgtacgg ttttaccaac
     1801 ttcaaaatgt taaatatttt ttccagttac tctacacagc aaacaggaat tagggcaaac
     1861 acccaaaccg atttttccaa atttaaagga tgtttggaag atgctgatat gattattctt
     1921 gcatggggta cagaccgcgg tgcttataag gatgaaaaaa acagaatact ggaatttcta
     1981 aaagcagaaa aatttatgga aaaagtattc tgtatttctg aaactggtaa ttccagcgat
     2041 acaagacatc cgtcaagaat tagttatagc tatcaacttg tacaatttga agagtcagca
     2101 tgaaaaacat atctctttct ccgcccgcca tgcttggcat cctcggcggc ggacaattag
     2161 gcagaatgtt taccgttgcc gccaaaacca tgggctacaa agtaaccgtt ctcgaccccg
     2221 atccggacgc gccggcagca gaatttgccg actgtcattt atgcgcgccg tttaacgacc
     2281 aagctgcttt ggacgaattg gcaaaatgcg cggcggtgac cactgaattt gaaaacgtca
     2341 atgccgatgc gatgcgtttt ctcgccaaac acaccaatgt ttcccccggc agcgactgcg
     2401 ttgccatcgc gcaaaaccgc attcaggaaa aggcatggat acgcaaagca ggattgcaaa
     2461 ccgcgccgta tcaagtggtt tgtaaggctg aagacattac tgaagcaagt gcgcaattct
     2521 tgcccggcat cctgaaaacg gccactttgg gctacgacgg caaaggtcaa atccgcgtca
     2581 aaacggtgga tgaactcaaa gctgcgtttg ccgaacacgg cggcgtggat tgcgttttgg
     2641 aaaaaatggt ggatttgcgc agtgaaattt ccgtaatcgt atgccgtttg aacaatgaca
     2701 acgtgcaaac tttcgaccct gccgaaaaca tccacgaaaa cggcatcttg gcttattcca
     2761 tcgtccccgc gcgactgagt gccgacgtgc agcaacaggc gcggcagatg gcgcaacgct
     2821 tggcggacga attggattat gtcggcgtat tggcggtaga aatgtttgtt gtcggtgaca
     2881 cacatgaatt ggtcgtcaac gaaatcgcgc cgcgtccgca caactccggc caccatacca
     2941 tcgatgcctg cgcggcagac caattccagc aacaggtccg cctgatgtgc aacctgccac
     3001 ctgccgatac caaattgctg agttcttgct gtatggcgaa tattttgggc gacgtttggc
     3061 aggaagacgg cggcaaaccg gattggtttc ccctgcaaag ccggccggac gcgcgcctgc
     3121 acctttacgg caaaaaaacc gcgcacaaag ggcggaaaat gggacacttt accgttttaa
     3181 ccaccgattc ggacaccgca tttcaagaag caaaaaaact gcatcagtcc ctataatccg
     3241 accaacggca cagatgccgt ctgaagccgc cgacatactg ccatgaacct gctgacactg
     3301 ttacgccccg ccaccgtgca agactgcaaa gacatcttca aagtccacct gcactccgtc
     3361 caatacacct gcatcctcag ctacaacgaa cacgccctaa aagtatggga aggcttgtta
     3421 aacacagaaa gctacctgcc gaccatttcc gatcccgaca aagcactttg ggttgccgaa
     3481 tacaaaggca acatacaggg atttttccaa atcgactgcc aagaagcaca actcgatgca
     3541 ctttacgtcc accccctgtt ccacaacctc ggactgggca ctgccctact tcatcaggcg
     3601 gaaaccatcg cccacaaatc cggtttgagc tttctgaaac tctacgcatc gctcaactcc
     3661 gtcccgtttt accttctcaa ccgctacgaa tcgctcggtt cggcagtatt gcagcttgac
     3721 cccagcatca aaatcaaatg cgaactgatg cggaaacacc tctgatccgt ccttataacc
     3781 aaaactgata atcttcaaat caaacattat ttccacaagc gcagacagcc gtataaagtt
     3841 gccccatcta ccaaccgaca ggacaagcat atgaaaacct acgcaccggc actctatacc
     3901 gcagccctgc tcaccgcctg cagccccgca gccgattcaa accatccgtc cggacaaaat
     3961 gccccggcca ataccgaatc cgacggaaaa aacattaccc tgctcaacgc ctcatacgat
     4021 gtggcacggg atttttacaa agaatacaac cccttattta tcaaaacata ccaatccgaa
     4081 caccccggca catccgtcag catccaacag tcccacggcg gctccagcaa acaggcatta
     4141 tccgtagcca acggccttca agccgatgtc gtaaccatga accaatcctc cgacatcgac
     4201 ctgctcgaaa aaaaaggact ggtagaaaaa ggctggcaac aagccctcct cgaccacgcc
     4261 gcgccctaca ccagcactat ggttttcctt gtccgaaaaa acaaccccaa acagatccgc
     4321 gattggaacg accttgccaa agacggcgtt aacatcgtca tcgccaatcc caaaacctcg
     4381 ggcaacggac gctacgcctt cctcggcgca tacggttacg gtctgaaaac caccaacggc
     4441 aacgaacagg aagcccaaaa actcgtcgca tccatcctca aaaacacccc cgtttttgaa
     4501 aacggcggac gcgccgccac caccaccttc acacaacgca acatcggcga cgtactcatc
     4561 acttttgaaa acgaagccaa ctacgtcagc aaaaaactga cccaaggaca gtttgaaatc
     4621 gtctatccga gctacaccat ttccaccgaa agccccgtcg ccgtcgtcaa cagcgtcgtc
     4681 gcgaaaaaag gcacgcaaaa aaccgcccgc gcctatctcg aatacctttg gagcgaaccg
     4741 gcgcaagaac tcgccgcctc actctacctg cgcccccgca accccgaagt attggcaaga
     4801 cacaaagccg acttcccaga cttagactca ttctctcccg aagaaaaatt cggcggatgg
     4861 gacaacatca t
//
LOCUS       DAWHRS010000143         4855 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWHRS010000143 DAWHRS010000000
VERSION     DAWHRS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.143"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..134
                     /locus_tag="WIB82_001775"
     CDS             <1..134
                     /locus_tag="WIB82_001775"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ3843040.1"
                     /translation="DAYLHEIAKHFDCTAATVCYALKQMGITRKKRPPLTKNKTRPK"
     gene            143..631
                     /locus_tag="WIB82_001776"
     CDS             143..631
                     /locus_tag="WIB82_001776"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ3843041.1"
                     /translation="MTQPAEFSDYQRVYLDETGFDRHLFRPYARSLKGQIVKAQISGK
                     RYRRLSLVSAQVGNRLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHR
                     MGVLREMAEKLGHKVLPLAPYSPELNPIEKVWANIKRYLRTVLSDYARFNDALLSYFD
                     FN"
     gene            complement(795..2198)
                     /locus_tag="WIB82_001777"
     CDS             complement(795..2198)
                     /locus_tag="WIB82_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ3843042.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(2267..3694)
                     /locus_tag="WIB82_001778"
     CDS             complement(2267..3694)
                     /locus_tag="WIB82_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ3843043.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3930..4283
                     /locus_tag="WIB82_001779"
     CDS             3930..4283
                     /locus_tag="WIB82_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843044.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            4274..4501
                     /locus_tag="WIB82_001780"
     CDS             4274..4501
                     /locus_tag="WIB82_001780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843045.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFSDKSNT"
     gene            4572..4817
                     /locus_tag="WIB82_001781"
     CDS             4572..4817
                     /locus_tag="WIB82_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843046.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
BASE COUNT         1181 a         1263 c         1174 g         1237 t
ORIGIN      
        1 aggatgccta tctgcatgaa atcgccaaac attttgattg tacggcagcc accgtttgct
       61 atgcactcaa acagatgggg ataacgcgca aaaaaagacc accacttaca aagaacaaga
      121 cccggccaaa gtaacgcatt atttgacaca gccggccgaa ttttctgact accaacgcgt
      181 ttatttggat gaaacaggat ttgaccgcca cctgttccgt ccctatgccc gcagcctgaa
      241 agggcaaata gtgaaagcgc agataagtgg aaaaagatac cgacgcttat ctctggtgtc
      301 cgcacaagtc ggcaaccggc tgattgctcc gatggtttat caaaatacga tgaccggagt
      361 cttttttgaa gcgtggtttc agcaatgcct actgcccgca ttgactcaaa aatcggtgat
      421 tattttagat aatgcacgat ttcaccgtat gggtgtctta cgggaaatgg cggaaaaatt
      481 gggacataag gtattgcctc ttgcacctta ttcacctgag ctcaacccga ttgagaaggt
      541 ttgggcgaat attaagcggt atctgcgaac cgtattgtct gattacgccc gatttaacga
      601 tgcactactg tcctattttg attttaattg aatatacatt tgaattaatt cgcacttaat
      661 ttaaatgtgt ttttaactgt gctttattta aaggcaatga gaatgtgaaa atatcggatc
      721 aatcccaaag cagcctgcac tttcgaaacg gggtgcaggc tgctttggga atttcataac
      781 cgtttcagcc tgctttattc cgcaaatacc gtttccaacc ctaacccgct ctctttcacc
      841 aagcgcaaat aagccagcat gaatttatac cgtgcttgag ccagtttctg ttctgcttgg
      901 gcgacttcct gccgcgcccg tattacttcc agccggttgc ggatgccgta ttgttggccg
      961 gtttcggtcg atttcagttt caaacggctg ctttccaaaa cccgttcttg cgccatgatt
     1021 tggtaacgcg ccgcaccgct ttcggtataa gcctggcgta cggcgagttt gatgtgccgc
     1081 tcggttgcgg tcagctgtgc ttcggcggcc ccgtattgcg cttcggcttc atggattttg
     1141 cccgacaatt ctccgccggt ataaagcggc aaattcaact gtacgccgac gctcatccct
     1201 ttgccccgat agtggtagtc attattctgc gcagatgaag tgtagaggtt attctgatag
     1261 ccgacatggg cagaaacggt gggatagcgg ctgttctgtg ctgcccgaag cgcctgtccg
     1321 ctgctttgca gggcaagctg ctgcatccgg tattcatgat tgttggataa ggcaatgcgc
     1381 tgccattcat ccagactgta acgttccagc ttgggcagat agcgtgccaa caggttggcg
     1441 gtatctatgg cctcgatttg tttgctatcc aggtcggtgt agtcgttcaa ctggttttca
     1501 taggtttgtt tctcagccaa tacggcgatt tcttgggcca gggcattgtc gtaaccggct
     1561 ttggcttcgt gaatatccag cgcggtggca gcacctttat tgaataaagc ctgcgcctgc
     1621 cttacctgct gggcataagc ctctttttcc gccgcatggg cggcaacggt gtctcggctg
     1681 agtaaaacgt tgaaataact ttcggcaact ttcaacagca attcttcgcg tgccgcatcg
     1741 aaacgctgtt ctgcagcctg cgtatcgaac ctgctttggc ggtattgtgc aaatttggca
     1801 gcgtcaaata aggtttgtcc cacctgcacg ctccatccct gtgtttcgcg ggtggaagaa
     1861 atcgatggcg gctggcgctg gtagctggca ttggcggata catggggaag gaatgcggcc
     1921 ttggcttgtt gttgccgtgc gcgcactgca tcacgctggt aatgggacgc ttgaaaatca
     1981 gccgaatgtt gctgcgccgc ccgccatgct tcaggcagcg taaaagccga aacggatggg
     2041 gaaagggata gtggcaaggt aaaaagtgaa acgggtagga tatatttgga aaaataggat
     2101 ttcatagccg aaaatagttc atgttgcaaa tagggcgtca gtgtcaggca aacggaaata
     2161 ccgtaatctt gcattatcat tagattgagc aatgtcatcc gggcaatggt ttcaggcagt
     2221 ctgcatgtcc gaaccggcgg ataacaaatg cccagtacgg atccgcctat cgctccctaa
     2281 agctttcgtc caatttggtt tgcagcgggc ttaacagata atccagcacc cgccgtttac
     2341 ccgttttaat ctccgccgtg acattcatgc ccgccgtcag attcactgct ttgccgtcaa
     2401 tattcaaggt atgtttgtcc agcgacacca ccgccgtata aaccaagccc aactgttcgt
     2461 ggcttaccgc atcatggctg acacttttca ccttgcccgt cagataaccg tagcgcgtat
     2521 agggaaagct ctcaatcttc accaccgcat cctgtccctg ttccacaaaa ccgatgtctt
     2581 tgttcaatac caaaacttcc acgtccattt tgtcgtcatc gggcgcaatc accatcattt
     2641 tttgggcagc ctgcaccaca ccgcccaccg tataggtagc caattcctgc accgtgccgt
     2701 ccgcaggcga ctgtattgtc atcagctgct gccgctgctt tgccttatcc gtttggccgc
     2761 ggtattggtc aatctgttcg tttgcctggc gcagcgcatc cagcgtatcg cgtttcaggt
     2821 tctgcgtatt cagcacccga ttctgctccg cctgtgcaat ggccgcctga atctgcctca
     2881 tctgaccgcg cgtactttcc aaatcgttcc aattgctgac cgatttgctc tgctgctcca
     2941 aaaacgcatg ttccgaaata aaattgtcgg cccgcaaacg gcggtagtct gctgttttct
     3001 gctgctcgat cgcccccacc gaaaccagct tctgctcctg cgccttggcc gactgcaatt
     3061 ccgcctgatg gccgcgcaaa gccgactgca attgcgcatc ctgcgccgcc catgcctgat
     3121 actggtgctg cgccaacacc tgcgccgatt gcacatcggc atcggagaga cctaaagacc
     3181 gtgcttgcgc catatcgata tgcggcacgg tacggctttc caatgccgcc aataccgctt
     3241 cataacgcag tttggacaat tgggcagcct gcaaagcctg ctccgactgc accacatcgc
     3301 tgtctgttcc cacagcctcc agttccgcca gcgtttctcc ctgtttcaca tgctgcccgt
     3361 cgcgcacatg taccgcctta accaccgccg tttccagcgg ctggatggtt ttgctgcgcc
     3421 cgcccgacac cgttttgccc gaagccgccg ccacaatatc gattttgccg aaccaggacc
     3481 acaacaaagc caaaagcgca aacgccataa taaaacgcgc cgcccatttc ggagcggcag
     3541 agaccggcgt atcggtcagt tccaaatgcg cgggcaaaaa cgcctgttct tccgccgtgc
     3601 gtttgggcgg tttcaactgg tcgcgcaccg cccaaacatt gcgccataca gtaatgtatc
     3661 gagaaagaaa ggatttcagg gcggagaaaa acataacggg tataaccttg gcaatataga
     3721 aacaggaaac aatataaata tgtaaaggaa ttttaacgga aagcgcggca gctgttaagg
     3781 gaaaggcggg aatattgaca aaaaataccc aagtcgttac aaatattcat tattttactg
     3841 cgtaacgcaa cgctgaagcg caggctgctt ttgagatgcg gcaaggttcg gcaaaaagca
     3901 gcctgcacat ttaaccacag gaacaaccca tgtttaccac aaacgattta cgccatttcc
     3961 tagaaggttt ggccatccta ttctcaatcg gctattgggg caccatgctg ctgttgcttt
     4021 ggtttctcgt ccgctttgcc tataaaaagc ccaaacggaa ccccggcaaa atcctttcag
     4081 gcagcctgac cgccgccgct atcctgatgc tgtttgtttg gattattccc aaacaattcg
     4141 gcccgatcaa agaagaaata caggcacaag aagagtggga cagaaaatac aaagaagccg
     4201 aagccgtgtt taacgaacaa tgcaaaacgg cgggggaaag atttaccaga cggcggacaa
     4261 tgtggaaggg attatgctgt tgaaggtagt acctgagcgt accgtttcgg cagatgcaaa
     4321 aaccagagac ccgatgtggg acaatgcggc tttacagacc agcgaaggcg taaattttat
     4381 tgctcgtttc ctaggatttt ttagcgatgg ggaataccgc tatgtggatg tcctgcaacc
     4441 caaccattcc gatattattc ggtattcagg taaagatttt tccgataaat caaatactta
     4501 atcatataca ccccgcccgt tatgcggtaa cgttcgaaaa caatgtcgat tccaagctgc
     4561 gcaggcactg ggtggcaggt gcgaccatac ggattatcga ccgccaaact gacgaagtga
     4621 ttgccaagaa aaccatctat gtctttgaaa aaggcttgga cggcacgggt ggggcgagaa
     4681 tgccgtggaa gtttgctatc ttgtgcaata aagaaagact tacttcttca gagccgttat
     4741 cggattttgt tcttagcgtt ttaaaacctt atatattgcg tcccttatat attgcgtccc
     4801 taagaaggga cgattaacaa aaattaacgt cctttacttt ctacaagtaa caggg
//
LOCUS       DAWHRS010000144         4822 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWHRS010000144 DAWHRS010000000
VERSION     DAWHRS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.144"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..487
                     /locus_tag="WIB82_001782"
     CDS             <1..487
                     /locus_tag="WIB82_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEZ3843047.1"
                     /translation="HALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLICTQ
                     LMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLLSLA
                     VMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFKRVE
                     N"
     gene            619..1575
                     /locus_tag="WIB82_001783"
     CDS             619..1575
                     /locus_tag="WIB82_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEZ3843048.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHAGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            1626..2408
                     /gene="trpC"
                     /locus_tag="WIB82_001784"
     CDS             1626..2408
                     /gene="trpC"
                     /locus_tag="WIB82_001784"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ3843049.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHNETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADDIEAEVGKLF"
     gene            2473..4773
                     /gene="recQ"
                     /locus_tag="WIB82_001785"
     CDS             2473..4773
                     /gene="recQ"
                     /locus_tag="WIB82_001785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ3843050.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADEAREIAD
                     RLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNILRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGWDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLGQNAV"
BASE COUNT         1108 a         1502 c         1284 g          928 t
ORIGIN      
        1 acacgcgctg attgcctatt ctttcggttt aatcggctta atcatgatta aagtgttggc
       61 acccggcttc tatgcgcggc aaaacatcaa aacgcccgtc aaaatcgcca tcttcacgct
      121 catctgcacg cagttgatga accttgcctt tatcggccca ctgaaacacg tcggactttc
      181 gcttgccatc ggtctgggcg cgtgtatcaa tgccggattg ttgttttacc tgttgcgcag
      241 acacggtatt taccaacctg gcaagggttg ggcagcgttc ttagcaaaaa tgctgctctc
      301 gctcgccgtg atgggaggcg gcctgtatgc cgcccaaatc tggctgccgt tcgactgggc
      361 acacgccggt ggaatgcaaa aggccgcccg gctcttcatc ctgattgccg tcggcggcgg
      421 actgtatttc gcatcactgg cggctttggg cttccgtccg cgccatttca aacgcgtgga
      481 aaactgaccg atgccgatat cctttttcgg caaacgcttt tccacgccta tattcgggcg
      541 cgcatccccc acgtccgcac cagccggacg ggctgccgca tcatcaatgc cgtctgaaaa
      601 caagaaaacc gataccttat gattttaaca ccgccggaca cgcccttttt cctccgcaac
      661 ggcaatgccg acacgattgc cgccaaattc ctgcaacgcc ccgcacccac ataccgccgc
      721 gagctgcttc ccgacagcac gggtaaaacc aaagtcgcct acgatttttc agacggcatt
      781 tcgcccgatg cgccgctggt cgtgctgttt cacggtttgg aaggaagcag ccgcagccat
      841 tacgcggtcg aactgatgct cgcggtacgc agccgcggtt ggcacggcgc agtcgtccat
      901 ttccgcagct gcggcggcat tgccaacacc gctccggtgt tctaccactt gggcgatacc
      961 gccgaaatcg cctttacttt ggacacgctt gccgcgcgtt accgtgaaat atacgccgtc
     1021 ggcgtatcgc tgggcggcaa cgcgctggca aaatatttgg gcgaacgggg cgaaaacgcg
     1081 ctgccgcaag ccgctgccgt catctccgca cccgtcgatg cagaggcggc aggcagacgc
     1141 ttcgacagcg gcatcacgcg gctgctctac acgcgctact tcctccgcac cctgataccc
     1201 aaagcacggt cgctccacgg ttttcagacg gcatttgccg cagggtgcaa aacactgggc
     1261 gagtttgacg accgcttcac cgcaccgctg cacggctttg ccgaccggca cgactactac
     1321 cgccaaactt cctgcaaacc gctgctcaaa cacgttgcca aaccgctgct cctgctcaat
     1381 gccgccaacg accccttcct gccgcccgaa gcgctgccga caccggacga agtgtccgaa
     1441 gccgttaccc tgttccagcc ggcacacggc ggacacgccg gctttgtcgg cagcaccggc
     1501 ggcaggctgc acctgcaatg gctgccgcag accgtcctgt cctatttcga cagcttccgc
     1561 acaaacaggc ggtaacggtt tgatgctaat attccccctt tccccggaca aatacggaac
     1621 acgacatgac cgacatcctc aataaaatcc ttaccgccaa ggcacaggaa gttgccgctc
     1681 aaaaagccgc cgtcaacgcc gaacacatcc gcgcacttgc cgcagaagcc gcgcccgtcc
     1741 gcagcttcat cggttcgata cgcggcaaac accgcctaaa cctgcccgcc gtcattgccg
     1801 aaatcaaaaa ggcaagcccg agcaaaggcc tgatccgtcc ggacttccga ccggccgaga
     1861 ttgcacgcgc ctatgaaaac gccggagcgg cgtgtttgtc cgtactgacc gacgaaccct
     1921 atttccaagg ttcgcccgaa tacctcaaac aggcgcgcga agccgtatcg ctgcccgtgc
     1981 tgcgcaaaga cttcatcatc gacgaatatc aggtttatca ggcgcgcgca tggggggcgg
     2041 atgccgtcct gctgattgcc gcagcactgg aacaggaaca attggaacgc tttgaagcgg
     2101 tggcgcacga attgggcatg accgtcctgc tcgagctgca caatgaaacc gaattggaaa
     2161 aatgccgcaa cctgaccacg ccgttgtggg gcgtaaacaa ccgcaacctg aggacttttg
     2221 aagtctccct cgaccaaacc ctgtcgctgc tgcccgcact ggaaggcaaa accgtcgtta
     2281 ccgaaagcgg cattacgggt aaggcggatg tggaatttat gcaatcgcgc ggcgtgcaca
     2341 ccttcctaat cggcgaaacg ttcatgcgtg ccgacgatat tgaggcggaa gtgggcaaac
     2401 tcttctaaat cccgatttca gacggcatat tgccgccaac cgaccaacac ccttaccgag
     2461 cacggaacca tcatgcaccg accgaccgcc aaacagattc tgcacgaagt atttggttat
     2521 cccgaatttc gcggcaggca ggaggctgtc atcaatactt tggcaggcgg cgggagtttg
     2581 acggtgctga tgccgacggg cggtggcaag tctttgtgtt accagattcc cgcgctgatg
     2641 cgcgaaggcg tggcggttgt cgtatcgccg ctgattgcgc tgatgaacga ccaagtggca
     2701 aacctgcacg ccgccggcat cgaagcggcg gcagtcaaca gcggcacatc ggcagacgag
     2761 gcgcgcgaga ttgccgaccg gcttgcccaa ggccgtctga agctgcttta tgtcgcgccg
     2821 gaacgcttgg ttaccgaccg ctttttgcgt tttctcgacc aacaaaccgt cagtctgttt
     2881 gccattgatg aggcgcattg cgtcagccaa tggggacacg atttccgccc tgaatatcaa
     2941 cagctcggca tgcttgccga acgctatccg aacgtcccgc gcatcgctct gaccgctacc
     3001 gccgatgccg ccacgcgcgc cgacatcaag cattatctgc acttggacga tgcgcccgaa
     3061 tttgtctcca gctttgaccg tccgaatatt tattatcagg ttatcgaaaa aaacaacggc
     3121 aaaaaacaat tgctggattt catccgcaaa gaaatgacgg ggcaaagcgg cattgtgtat
     3181 tgcctaagcc gcaaaaaggt ggaagatgtg gcgcagtttt tgcgtgaaaa cggattaaac
     3241 gcgattccgt atcatgctgg tttgagcatg gacgtacgcg aggaaaacca acgccgcttt
     3301 acgcatgaag acaatattat cgtggtggcg accgtggcgt tcggcatggg catagacaaa
     3361 cccgacgtgc gctttgtcgc ccatctcgat atgccccaga gtgtcgaaca tttctatcaa
     3421 gaatcggggc gcgccggacg ggacgggctg ccggccgcaa gctggctgtg ttacggtttg
     3481 aacgattggg tgttgctgcg cgaacggatt gccgaaggca acagcgacga ggtgcaaaag
     3541 caaatcgaaa tgcaaaaact cgatgccatg cttgccgtct gcgaaaccgc cgcctgccgc
     3601 cgcgtactgc tgctcaaaca tttcggcgaa gcatccgaac cctgcggcca ttgcgacaac
     3661 tgcctgcatc cgcccgtacg gtttgacggc acggtgttgg tgcaaaaatt actcagctgc
     3721 gtgtaccgcg ccggacaacg ttttgccgcc ggttacatca ccaacatttt acgaggtaaa
     3781 agcgacgatt ggatacgcgg caaccggcac gaacaactgt ccacattcgg catcggtacg
     3841 gagttgtccg acaaagaatg gcgcagcgtc atccgccagt gtatcagcct cggctacctc
     3901 accgtcaaca ttacccgata tcaggcattg caactgaccg aagccgccaa aaaagtcctc
     3961 aaaggcgaaa ccgaagtgat gctgcgtccg ctcaagcgcg acaagcccgc cgcccgcacc
     4021 ctcaaagaca actggctgcg taccgaacgc gaagaacgcc tgtggcaggc attgcgcgtt
     4081 tggcgtatga aacaggcaga agccgaaggc atccccgcct atatgatttt cggcgacaaa
     4141 accctgcgcg accttgtcga aaaaatgccg caaaacctca acgggctgca cgacatctac
     4201 ggcttgggcg aagccaaaac cgaacgtttc ggacacggca tactcgaagt ctgccgcaac
     4261 gccgccggct tcagccgcga cgcggtcatc cgtccgcaaa ccgaacgcga acaacaactg
     4321 cgtcaaaaac tcgaggcctg gcggtatgaa caggcaaggg cggaaaactg cgccctgcat
     4381 gccgtcctct ccgacgaaag ccttgccgat atgcttgccg ccacgcccga aaccgaaacc
     4441 gacctcgaag gcgtacacgg cttgggcagc gtacgcgccg ccaaatacgg atgggacatc
     4501 ctcgccgtct gccgtccgtt ttcagacggc atcgatgaaa ccgccaaaca caaacgccgc
     4561 ctgatgcgcg ccctgattca atggtgcaac gaaaccgcaa aacacgaaca gtccgaaccc
     4621 taccgcattc tcagcaaagc cgccctacgc gccattgccg ccaaacagcc ggaaggtttg
     4681 gcggagcttg ccgccgtata tggcgtaggc gaagaaaaag ccgcacgtta cggtgcggcg
     4741 gtgttggcgg tgttgggaca aaatgccgtc tgaaaaccgt taccaagttt cagacggcat
     4801 tgcctctatt taaaaattcc tg
//
LOCUS       DAWHRS010000145         4811 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWHRS010000145 DAWHRS010000000
VERSION     DAWHRS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.145"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            26..1363
                     /locus_tag="WIB82_001786"
     CDS             26..1363
                     /locus_tag="WIB82_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ3843051.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
     gene            complement(1598..2194)
                     /locus_tag="WIB82_001787"
                     /pseudo
     CDS             complement(1598..2194)
                     /locus_tag="WIB82_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(2205..2999)
                     /locus_tag="WIB82_001788"
     CDS             complement(2205..2999)
                     /locus_tag="WIB82_001788"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ3843052.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQTAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            3383..4717
                     /gene="gdhA"
                     /locus_tag="WIB82_001789"
     CDS             3383..4717
                     /gene="gdhA"
                     /locus_tag="WIB82_001789"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ3843053.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT         1149 a         1282 c         1198 g         1182 t
ORIGIN      
        1 atcttcacat ttcttaataa attttatgag taaccatact tcttggtcgt ccaaaatcgg
       61 tttcgtactt gctgcggcag gttcggccat cggtttgggc gcgatttgga aattccctta
      121 tacggcgggc accaacggcg gcgcggtgtt tttcctgctg tttttgatat ttactatctt
      181 ggtcgccctg cccgttcagc ttgccgaatt ttatatcggg cgcacgggcg gtaaaaatgc
      241 cgtcgattcc ttcagggctc tgcgtccgag tacgcaatgg ctttgggtcg ggcgcatggg
      301 cgtttccgcc tgctttattt tgctgtcgtt ttacagcgtg gtcggcggat gggtattaaa
      361 ttatgtcgtc cacagtttca cgggggcggt tcataccggc gcggactttg aagccttgtt
      421 cggcgcgacg atttccaatc cggcaggttc gctgtcctat caggcactgt ttatgctgat
      481 tacggtttgg gtggtcaaag gcggcatttc agacggcatt gaaaaggcaa accgttatct
      541 gatgccgggg ctgtttatcc tctttattgc gctggcaatc cgttcgctga cgctgccggg
      601 tgcaatggag ggcgtgtctt tcctgctcaa accgaattgg tcgtacttta aagccgatac
      661 gatgattacg gctttaggtc aggcgttttt tgccttgagc atcggcgttt ccgctatgat
      721 tacctatgct tcgtatttgg gaaaagatca ggatatgttc cgctcggggc atacgattat
      781 gtggatgaac ctcttggttt cgctgttggc aggtttggtg atttttccgg cggtgttcgc
      841 cttcggtttt gaaccgagcc aggggccggg attgattttt atcgtattgc cggcggtgtt
      901 tatgaaaatg ccgttcggta cggttttgtt tgcggtattt atgctgctgg tcgttttcgc
      961 cacgctgact tcggcatttt cgatgttgga aacggtcatt gcctcaacca tccgccaaga
     1021 cgagcgcaaa cgcaaaaaac atacttggct tatcggcacg gctattttta ttgtcggcat
     1081 cccgtccgcg ctgtctttcg gcgtatgggg tgagtttaag gttttcggca aaaccatttt
     1141 tgatttgtgg gactatgtta tttccgccgt cattatgcct gtcggcgcgt tgggcgttgc
     1201 cgtatttacc gcctggattc aggacaagca gtctgtgtta aaagatgccg gcgcgggcag
     1261 caccgtacca cgggcagtgc tgctgctgtg gctgaatacc ttgcgctacc ttgccccgat
     1321 tgccattatt attgttttcg tcaattcttt ggacatcctt taaaagccat ccaaacagca
     1381 aaaatgccgt ctgaaagcct ttcagacggc atttttgctt caggttcagc ctatttcgtt
     1441 caaagtatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat
     1501 agtacagaac cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagctaa
     1561 ggcgaggcaa cgccgtactg gtttttgtta atccactata gacttgcgcg atgccgttca
     1621 aggacaaacc catacccttt tcggcaaaac ggatttcacg gtcgtcaaac gagactttgc
     1681 cgaagccgac ccgtttcagg gcttccttca cgctgttttg aggcggcggc gtttccgcat
     1741 cgggatgagc ggcaaaataa tcggcataca gtttccacaa aacctgaacc gtcggatcga
     1801 atgccccttc cgtcagcgcg tgaatatcgc ggcacaggct caacagttcc aaaaaatccg
     1861 ccggcggcga agtcagataa ccgtcacggt tcagacggct gatcaggctg tcttcacggt
     1921 aaaggctgaa cattttttcc aaacgcgcca cttccgccaa aaccttgttg accaaatccg
     1981 ccgcacgcct gtcgtccaca ccgaacagac ggagctccgc accggaaccc agtgcgacac
     2041 ctttccagaa aaacacattt tcattgcgtt tttcatcccc gttgcgtttt tcatcatcgg
     2101 cggcaaaagg attcggcagg aaagaaaccg ccgcgcccgc cgccgcaacg gcggcaaccg
     2161 tcagaaaacg cctgcgcccg aaatgcctgt ccatataccc ctcctcagac cgacactgcc
     2221 gccttgatgt gcggatgggg gtcgtagcct tccaactcga aatcttcaaa cttgaaggaa
     2281 aacaaatctt tgacttcagg attgattttc atcactggca aggcgcgcgg ttcgcgttcc
     2341 aactgcaatg cggtctgctc gaaatggttg cggtacaaat gcgcgtcgcc aaacgtatgg
     2401 acaaactcgc ccgcctccag cccgcacact tgcgccatca tcatggtcaa caatgcgtag
     2461 ctggcaatat taaacggcac accgaggaaa atatccgcgc tgcgctggta aagctggcag
     2521 gacagtttgc cgtcggcaac gtaaaactga aacagcgcgt ggcagggcgg caaggccatt
     2581 tcatcgacca aagccggatt ccacgccgat acaatcaggc ggcgcgagtc ggggtttttc
     2641 ttaatttgtt ccaccacatt ggcgatttgg tcgatatgcc tgccgtcggg cgcgggccag
     2701 ttacgccact ggtagccgta aaccgggcct aagtcgccgt tttcgtccgc ccactcgtcc
     2761 caaatggaaa cattgttgtc ctttaggtat ttgatattgg tatcgccttt gagaaaccaa
     2821 agcagctcgt ggataatcga gcgcaaatgc agctttttgg tcgtcagcag cggaaaacct
     2881 ttgcccaagt caaaacgcat ctgataaccg aatacggagc gcgtgcccgt accggtgcgg
     2941 tctgatttgt ccgtaccgtt gtcgaggacg tggcgcatca agtccaaata ggctttcata
     3001 gcagtctttc atcaaattaa acggcgcata ttgtaacatt tccggataat gcccaaaaca
     3061 cggatacagg caggcaggat tgttggcaat ttcagtcctt ttccacagta aaacccagtg
     3121 ggaaaacaaa attaccttga ttggaatcaa aaaatctagt ttaattactt agaataaaat
     3181 ttcaataata tttttattta cgaaattaat ttatgattat tgattaatta tcggcaacaa
     3241 acatcaaaca tcgaaaacat ggaaaaaata atgtcaacaa tttttgccaa atcgggcttg
     3301 gcatcagaaa aaaataggtt tatattccca cctacaaatt tgttttccca ttagtacact
     3361 atcaaccaaa aggagtatcc gaatgactga cctgaacacc ctttttgcca acctcaaaca
     3421 acgcaatccc aatcaggagc cgttccatca ggcggttgaa gaagtcttca tgagtctcga
     3481 tccgtttttg gcaaaaaatc cgaaatacac ccagcaaagc ctgctggaac gcatcgtcga
     3541 acccgaacgc gtcgtgatgt tccgcgtaac ctggcaggac gataaagggc aagtccaagt
     3601 caaccggggc taccgcgtgc aaatgagttc cgccatcggt ccttacaaag gcggcctgcg
     3661 cttccatccg accgtcgatt tgggcgtatt gaaattcctc gcttttgaac aagtgttcaa
     3721 aaacgccttg accaccctgc ctatgggcgg cggcaaaggc ggttccgact tcgaccccaa
     3781 aggaaaatcc gatgccgaag taatgcgctt ctgccaagcc tttatgaccg aactctaccg
     3841 ccacatcggc gcggacaccg atgttccggc cggcgacatc ggcgtaggcg ggcgcgaaat
     3901 cggctacctg ttcggacaat acaaaaaaat ccgcaacgag ttttcttccg tcctgaccgg
     3961 caaaggtttg gaatggggcg gcagcctcat ccgtcccgaa gcgaccggct acggctgcgt
     4021 ctatttcgcc caagcgatgc tgcaaacccg caacgatagt tttgaaggca aacgcgtcct
     4081 gatttccggc tccggcaacg tggcgcaata cgccgccgaa aaagccatcc aactgggtgc
     4141 gaaagtactg accgtttccg actccaacgg cttcgtcctc ttccccgaca gcggtatgac
     4201 cgaagcgcaa ctcgccgcct tgatcgaatt gaaagaagtc cgccgcgaac gcgttgccac
     4261 ctacgccaaa gagcaaggtc tgcaatactt tgaaaaccaa aaaccgtggg gcgtcgccgc
     4321 cgaaatcgcc ctgccctgcg cgacccagaa cgaattggac gaagaagccg ccaaaaccct
     4381 gttggcaaac ggctgctacg tcgttgccga aggtgcgaat atgccgtcga ctttgggcgc
     4441 ggtcgagcaa tttatcaaag ccggcatcct ctacgccccg ggaaaagcct ccaatgccgg
     4501 cggcgtggca acttcaggtt tggaaatgag ccaaaacgcc atccgcctgt cttggactcg
     4561 tgaagaagtc gaccaacgcc tgttcggcat catgcaaagc atccacgaat cctgtctgaa
     4621 atacggcaaa gtcggcgaca cagtaaacta cgtcaatggt gcgaacattg ccggtttcgt
     4681 caaagttgcc gatgcgatgc tggcgcaagg cttctaagca aacgccgccg ctccgcaaac
     4741 aaaatgccgt ccgaaccgca aatgctgttc agacggcatt tcattatccg cccgttcgaa
     4801 tcgggtgata c
//
LOCUS       DAWHRS010000146         4708 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWHRS010000146 DAWHRS010000000
VERSION     DAWHRS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.146"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            85..181
                     /gene="ffs"
                     /locus_tag="WIB82_001790"
     ncRNA           85..181
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WIB82_001790"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            348..2039
                     /gene="dld"
                     /locus_tag="WIB82_001791"
     CDS             348..2039
                     /gene="dld"
                     /locus_tag="WIB82_001791"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ3843054.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKVMQFVSKFLPDHLPKSMRDYRNKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            2171..2278
                     /locus_tag="WIB82_001792"
                     /pseudo
     CDS             2171..2278
                     /locus_tag="WIB82_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2334..2705
                     /locus_tag="WIB82_001793"
     CDS             2334..2705
                     /locus_tag="WIB82_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ3843055.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            2860..3408
                     /locus_tag="WIB82_001794"
     CDS             2860..3408
                     /locus_tag="WIB82_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ3843056.1"
                     /translation="MARLTVHTLETAPEAAKACVEAVLQNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            3544..4635
                     /locus_tag="WIB82_001795"
     CDS             3544..4635
                     /locus_tag="WIB82_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ3843057.1"
                     /translation="MNAQTLLTNVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGKVLAGTGMSNTVKHLVGIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNSYLDDGYDSLKARLD
                     GAWAETERLADLAWDGAPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT         1224 a         1425 c         1188 g          871 t
ORIGIN      
        1 aaattttagg tggtgtttcc ggcgtatgac cgtatcaaaa cggttgagtt tttcagaaaa
       61 cccgttataa tgccgtttct ctgaggcggg tctccccgca tggcaaatcg gaacaccggg
      121 tcaggggcgg aagccagcag cccactccaa tgcgccagtg ccgggggttt ggcccgccgc
      181 cctatttgaa acgccggagc ttcgatgttc cggcgttccc gtatcgcacc gatgccgtct
      241 gaaagccgtt cagacggcat tttctttact ttgccataac gtataactac aatgtgaaaa
      301 caattccaat gtgcaatgca cctgccgaca attttaagga aactgccatg agcgcatcac
      361 aattactgtc aagactgacc caaaccgtag gcgaaaaata catcatcacc gaccccgcga
      421 aaaccgaaca ataccgccaa ggttaccgct tcggcgaggg taaggcgttg gcggtggttc
      481 gccccggaag cattctggaa atgtggaaaa ttctgcaggc gtgcgtcgaa gcagacgtga
      541 ttgtgattac gcaggcggcg aataccggtt tgacgggcgg ctcgaccccc gacggcaacg
      601 attacgaccg cgacatcgtg attgtgaaca ccatgcggat gaacatcatc caaaccatca
      661 acaacaacga acaagtcgtc tgcctgcccg gctcgaccct gaaccagctt gaattgctgc
      721 tgaaaccttt ggggcgcgaa ccgcattcgg tcatcggctc atcctgtatc ggcgcgtccg
      781 tcttgggcgg cgtgtgcaac aactctggcg gcgcgttggt acagcgcggc ccggcctaca
      841 ccgaaatggc gttgttcgcc caaatcaacg aagaaggcag gttggaactg gtcaaccact
      901 tgggcataga cttgggcgat acgccggaag aaatcctgac caacctgcaa ggtcatcatt
      961 atcaaaacaa agacatcaaa caagacgcgg gcaaaggaca cgaccacgcc tattgcgaac
     1021 acgtccgcca agtggacgaa ccgaccgccg cgcgtttcaa cgccgacccc gcgcgccatt
     1081 acgaagcctc gggctgcgcg ggcaaactga tggtgttcgc cgtccgtttg gacaccttcc
     1141 cgcaagaaaa acaaaccgcc gtgttctaca tcggcaccaa cgacatcaac gagctgaccg
     1201 acatccgccg cgccgccttg ggcgaatttg aaagcctgcc cgtttccggc gaatacatcc
     1261 accgccacgc tttcgatatt gccgacgtgt acggcaaaga cacgttttac gtcatcaaaa
     1321 aattcggtac gcaccaactg ccgaaattat ttgatttgaa ggcacgcgtg gacaggttcg
     1381 gcaaaaaagc cagcttcctg cccaagcatt tttccgacaa ggtcatgcag ttcgtcagca
     1441 aattcctgcc cgaccacctg cccaaatcca tgcgcgatta ccgcaataaa tacgaacacc
     1501 acctgattct gaaaatgggc gggaaaggcg tggatgaggc gcgcgcattc ttaaaagaat
     1561 atttttcaca ccacggcggc gcgtttttcg aatgcaacgc cgaagaaacc caagccgcga
     1621 tgctgcaccg tttcgccgtc gcctccgccg ccatccgcta ccgcgccgtg catgatgacg
     1681 aagtggaaga cttggtcgcg ctcgacatcg ccctgcgccg cgacgatcgc gactggtttg
     1741 aaaaactgcc gtcggaaatc gacaataaaa tcatccataa actctactat ggacatttca
     1801 tgtgccacgt tttccatcag gattacatca tcaaaaaagg caacgactgc atggcgttgg
     1861 aacacgaaat gctgcatctt ttagaccaac gcggcgcgca atatcccgcc gaacacaacg
     1921 tcggtcattt gtatgaagcc aaacccgcgc tcaaacagtt ttaccgcaaa cttgacccga
     1981 ccaacagctt caacccgggc gtaggcaaaa ccagcaaaaa gaaaaactgg gcggaataag
     2041 cgcgtccgct ttgaaggcag gcaatgccgt ctgaaggcaa aacgccgttc agacggcatt
     2101 gtcgggtttg aagttccccg tcgggtaatc gctttcaaac cgtatgccga cacacgggcg
     2161 cgcccctccc aaatatagtg gattaaattt aaatcaggac aaggcaacga agccgcagac
     2221 agtacagata gtacggcaag gcgaggtaac gccgtactgg tttaaattta atccactata
     2281 aagtctgatc ctaccttccc aaagggcagg gtttcaaccg aaaaggaaac acgatgaaac
     2341 catacaaaat ctacacccat cccgccctgc cgccccaagc cgtcaaacaa ggctggtcgt
     2401 ggccgggcct tttgttcggc acgctgtggg cgtgtttcaa aagaatgtgg ggtttggggc
     2461 tggggctgac gggcgcgata ttcgtgctgg ctgtgtttgc ccaactggtt tacggcgaca
     2521 cgcccgccac agactcggcg tttaacgtat tgggcttggc ggtttccgtc tggttcggcg
     2581 caaagggcaa cagcctttat gcacgccacc ttttatcgcg cggctatacc gaattgcccg
     2641 aaacggttga ggcagccaat ccccaagccg cattggcgca atatttcggg cgcgggggcc
     2701 gataagcgcg ttttcttttc catcatgccg tctgaaaaat gttcagacgg catttcttat
     2761 aaccttttgt ttgcagcaca taaccaatca gtctttccta ttccgttccg aaatgcatac
     2821 catgcgcccg tccccaatat ttgcgaacaa ggaaagaaaa tggcacgttt aaccgtacac
     2881 acccttgaaa ccgcccccga agccgccaaa gcgtgcgtag aggcggtact tcaaaacaac
     2941 ggcttcatcc ccaaccttat cggcgtattg gcaaacgccc ccgaagcctt ggcgttttac
     3001 caagaagtcg gcaagctcaa cgccgccaac agcctgactg ccggcgaagt cgaagtcatc
     3061 cagatcatcg ccgcccgcac caaccaatgc ggcttctgcg tggcagggca taccaaactg
     3121 gcaaccctga aaaaactcct ttccgaacaa tccgtcaaag ccgcgcgcgc tttggcggca
     3181 ggcgaatttg acgatgccaa actcggcgcg ctcgccgcct tcacccaagc cgtgatggcg
     3241 aaaaaaggcg cggtatccga cgaggaactc aaagcatttt ttgatgcagg ctacaatcag
     3301 cagcaggcgg tcgaagtcgt gatgggcgta gccttggcaa ccctgtgcaa ctacgtcaac
     3361 aacctcgccc aaaccgaaat caaccccgaa ttgcaggcat acgcctaagc aaacaaaacc
     3421 gcctgaatgc aggcggtttt tcaaaaacac cccctcaaac aaaaacaagc cgcccaagcg
     3481 gcaggggaag cagccgcgca tcaggcgcgc ccccgattcc ctcgcaaacc ttaaggaaca
     3541 aagatgaacg cccaaaccct acttaccaac gttgccgaac tcgtcaaaac caagctcaaa
     3601 cccatagtgg acgacatcga ccgcaaagga tactaccccg aagcatttat gcgcgaactc
     3661 ggcgcaatcg gcggattcgg cgcagtcggt acagaagccg aaggcggcaa cggcttgggt
     3721 ttggcaacgc aaatcgctgt tttgcgcgaa atcggcaaag aatgcggcgc aacctccttc
     3781 agcgcgtggt gtcaggcggc ttgcgcgtgg tatctgcacc aaacgcccaa ccaagccgtc
     3841 aaagacaaat acctcgccga catcctgcaa ggcaaagtat tggcaggcac tggaatgtcc
     3901 aataccgtca aacaccttgt cggcattgaa aaacacaacc ttcaagccga gcgcgtggag
     3961 ggcggctaca cagtcaacgg cgcgttgccg tgggtgtcca acatcggcga agaccacatc
     4021 tgggcgaata ccgcccaaat cggcggcggc tacgttatgt tcatcacagg cgggcaaagg
     4081 gaaggcgtaa gcctgcaaaa ctgccccgaa ttttgcgccc tcgaaggcac gcgcaccttc
     4141 agcctgaact tcaaagacgt atttatcccc gacgaagaca tcatcgccgc gcccgaacag
     4201 tttgccgact acatccaaag catcaaagca ggcttcatct tgctgcaaat cggcatcggc
     4261 gcgggcgtga ttgacggcag cctcggcatc atccgcctcg ccaacgtcgt caacgccgaa
     4321 gtcaacagct atctcgacga cggctacgac agcctcaaag caaggttgga cggcgcgtgg
     4381 gcagaaaccg aacggctcgc cgacttggcg tgggacggcg cgcccgataa cctcgccacc
     4441 ctcaagctgc gcgaagccgc cgccgtactg gcacttgccg ccgcccaatc cgccgccctg
     4501 cattccggcg caaaaggcta cctgatgcgc agtcccgccc aaaggcgcgt ccgcgaagca
     4561 atgtttgtcg ccatcgtaac ccccgcgatc aaacacctgc gcaaagaaat agcggcaatc
     4621 gaagccgcaa aataaacagg cggaaaaaac agaaaccccc accgcgtcat tcccgcgaaa
     4681 gcgggaatcc agcccccaac gcaacagg
//
LOCUS       DAWHRS010000147         4616 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWHRS010000147 DAWHRS010000000
VERSION     DAWHRS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.147"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(66..209)
                     /locus_tag="WIB82_001796"
     CDS             complement(66..209)
                     /locus_tag="WIB82_001796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843058.1"
                     /translation="MPSENLSDGIFLPKQPYFFIRAAKFYPKQQQSFHRHSRAGGNLE
                     RKI"
     gene            complement(227..1165)
                     /locus_tag="WIB82_001797"
     CDS             complement(227..1165)
                     /locus_tag="WIB82_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3843059.1"
                     /translation="MLELNGLCKRFGDKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NIIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAEHLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRNGGIPAVLVTHSPEEACTTADEIAVMHKGRILQYGTPET
                     LVKTPSCVQVARLMGLPNTDDNRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRHAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1174..2049)
                     /locus_tag="WIB82_001798"
     CDS             complement(1174..2049)
                     /locus_tag="WIB82_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ3843060.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSPEWAERVQKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAALPETFLPENQDGEVACFEKMDIGSLVAAMLSENMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2231..2307)
                     /locus_tag="WIB82_001799"
     tRNA            complement(2231..2307)
                     /locus_tag="WIB82_001799"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2271..2273),aa:Met,seq:cat)
     regulatory      2457..2556
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2648..4549
                     /gene="thiC"
                     /locus_tag="WIB82_001800"
     CDS             2648..4549
                     /gene="thiC"
                     /locus_tag="WIB82_001800"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ3843061.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRG
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHINLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGTERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1114 a         1319 c         1213 g          970 t
ORIGIN      
        1 tcattcccgc gcaggcggga atctaggtct gtcggcacgg aaacttatcg ggaaaaacgg
       61 tttctttaga ttttacgttc tagattcccg cctgcgcggg aatgacgatg aaaagattgt
      121 tgttgtttcg gataaaattt tgcagccctg ataaaaaaat atggctgctt tggtaaaaaa
      181 atgccgtctg aaaggttttc agacggcatt ttgtttttaa gaagcatcag cggaagcgga
      241 cgatttcccg ttcttcgata tggatgcgta ccgtatcctt gcccgatacc gccccggcgt
      301 gccgcatatc gaggttcagc cacaggatgc cgtgttccgg atggaggacg gacaggctga
      361 acgattcggg caaacaggta cgggataata cgcggcactc catgccgtct tggtcgaaac
      421 gcaccgcatg ttgcggaata tggcggttat cgtcggtatt gggcaaaccc atcagtcggg
      481 cgacctgcac gcaggatggt gttttgacca atgtttcggg cgtaccgtat tgtagaatcc
      541 tccctttatg catcacggcg atttcgtctg ccgtcgtaca ggcttcttcg ggcgaatgcg
      601 ttaccaaaac ggcagggatg ccgccgtttc ggatacgttc ggcagtcata cggcgcagcg
      661 tgccgcgcaa atgcgtgtcc aaactggaaa acgattcgtc caacagcagc agggaagggc
      721 ggacaaccaa agcgcgcgcc aacgccagcc gttgcttctc gcctccggaa agtttttcag
      781 gcttgcggtg cgcctcgttt tccagtccga cttcggcaag tgccgccatg gcgaggtgtt
      841 cggcttcggc tttcggcatt ttttgcattt tcaaaccgaa tgccgcattt tccagcgcac
      901 tcatatgggg aaacagcgcg taatcttgaa acatcagcga gatacggcgt ttttcgggcg
      961 gcatacgggt aatgttttct ccgttcagcc atatttcccc gccgtccggc cggacaatcc
     1021 ccgcaattat attcagcagg gtggattttc cgcagcccga ccgccccaaa acggcgagta
     1081 ttttgccgcg cccgacagtc aggcagatgt tgtcggcgac ggttttatcg ccgaagcgtt
     1141 tgcagagtcc gttcagttca agcatagcgc atcctataaa cgtatgccgt ccagccactc
     1201 ggacagcgga tgggcggcat caatcagacc gtaacggcaa aacgcgtcca gcgtaaccag
     1261 ttgcgcgtcg tgcatcatgt tttccgacaa catggcggca accaggctgc cgatgtccat
     1321 tttctcaaaa cacgcaacct cgccatcctg attttcaggc aggaaggttt cgggcagagc
     1381 ggcatcgaat acatacagga tttcattgtg gacgccgcgg ctgacggggc gcaggctgtg
     1441 cagccgggat accggacgga taaacggaaa cagcgtttta tccaaaccgg cttcttcgct
     1501 gctttcgcgg cacacggctt cagacggcat ttcgccgccg gaaacaccgc cggcggcaat
     1561 attgtcgagt ttgtcgggat cgactgcttt gtgcggactg cgcttgccta tccagaaatg
     1621 ccatcggccg tccgattcgg tcagtccgtt gagatggacg gcgcggctga gcagtccgaa
     1681 cggacggaaa gcggcgcgtt cgagcgtgaa caaggggttg ccgccgccgt cggtcaggtc
     1741 gaaacactcg ttgcgccatc cgtgaagcag ccccgccttg ttccatgtgc gggcgaggtg
     1801 ctgcaagcgg ccgcccatat ctgaccagcc gtccgcattc agaaaaatgc cgtctgaaga
     1861 ctccgagcag cctgcctccc agtctttctg aacgcgctcc gcccattccg gcgacaggtt
     1921 tcccaaaggc aggccgttca gatacagcgt tttccagcaa ctttccgcac cgtaacttgc
     1981 ctttgcccac tcgaataggg catcaaggtc gtgtttgctg acggattcgg taaaacggac
     2041 ggtcggcata gtcggctttc ttgaacataa tgcggaagat tgtaatcaaa ccgcctgaat
     2101 ttgcaactgc aaacagacgg cgggctgccg ttccgatatt gcggcaggaa ggaaaatccg
     2161 gcaaaaaacg gaggcggcag gaaacaggca aaacaaaacc cctcggcatt gaaccgaggg
     2221 gtttgatatt tggttgcggg agcaggattc gaacctacga ccttcgggtt atgagcccga
     2281 cgagctacca tgctgctcca tcccgcgtca gaagatgaga ctatacggca gattttttat
     2341 gttgtcaaac attacttccg cgaaaaatca taaaattttg ccgtccggcg gtttatgcct
     2401 gatttgacat attattttct ttacaaaagt tcatgttcgt gatttaattt tggttaacat
     2461 cgaaacaggg gtgctgcctg atgttcaggc ggctgagaaa taccctttac acccgatcgg
     2521 gataatacct gcgtggggag ttttcacgga ttctgtcttt cagacggcat tggttttcaa
     2581 atgccgtctg aaaacgcaaa acgctcctgt ttctttattc taaacgagaa aacaggagca
     2641 tttttttatg actacgccaa aaaaaaccgc caaaacttcc ggcaacgaag cgcgcgagct
     2701 tgccgacttg agcgaagaca tcggcatccg cttcaaatat ccgaactcgg aacgcgtgta
     2761 tctgcaaggc agccgtggcg acatccgcgt gcctttgcgc gaaatccgtc aggacgacac
     2821 ctatacggcg caaggtacgg aagccaatcc gccgattccc gtctatgaca ccagcggcgt
     2881 gtacggcgat ccggcggcac acatcaattt aaaacaaggt ctgccccaca tccgcacggc
     2941 gtggctggac gaacgcggcg ataccgaaat cctgcccaaa ctctccagcg aatacggcac
     3001 cgaacgcgca cacgatccga aaaccgccca tctgcgtttc aaccaaatca cccgcccgcg
     3061 ccgcgcgaaa agcggcagca acgtaaccca gcttcactac gcgcgccaag gcattatcac
     3121 gcccgaaatg gagtttgtcg ccatacgcga acgtttaaaa ttagacgaat tgtcccaaaa
     3181 atcggaatac gccaaactct tgaaacaaca cgcagggcaa agtttcggcg cgaacatccc
     3241 aacccgtccc gaccaaatca cgcccgaatt cgtgcgccaa gaaatcgccg ccggacgcgc
     3301 gattatcccc gccaacatca accaccccga actcgaaccg atgattatcg gccgcaactt
     3361 ccgtgtcaaa atcaacggca acttgggcaa ttccgccgtc acttccagcc tgaccgaaga
     3421 agtcgaaaaa atggtgtggt cgctgcgttg gggcgcggac acaattatgg acttatccac
     3481 aggcgcgcac atccatgaaa cgcgcgaatg gattatccgc aacgcgcccg tccccatcgg
     3541 caccgtgccc atctatcagg ctttggaaaa aaccggcggc attgccgaag atttgacttg
     3601 ggatttgttc cgcgacacct taatcgagca ggcggagcaa ggcgtggact atttcaccat
     3661 acacgcgggc gtgttgctgc gttatgtgcc gatgaccgcc aaccgcctca ccggcatcgt
     3721 ctcgcgcggc ggttcgatta tggcgaaatg gtgtttggca catcatcggg aaaatttcct
     3781 ctacacgcat ttcgacgaaa tctgcgaaat tatgaaagcg tatgacgtat cgttcagcct
     3841 cggcgacggc ctgcgccccg gctgcattgc cgatgccaac gacgaatccc aattcgccga
     3901 actgcacacc ttgggcgaat tgaccgataa agcgtggaaa catgacgtac aagtcatgat
     3961 cgaaggcccc ggccatgtgc cgctgcaacg cgtcaaagaa aacatgaccg aagagctgca
     4021 acactgcttt gaagcacctt tttacacgct cggcccgctc gttaccgaca tcgcacccgg
     4081 ctacgaccac atcacctcgg gcataggcgc ggccaatatc ggctggtacg gcacggcgat
     4141 gctttgttac gttaccccca aagaacattt ggggctgccc gacaaagaag acgtgcgtac
     4201 cggcatcatc acctacaaac tcgccgccca cgccgccgat ctcgccaaag gctggccggg
     4261 cgcacaatta cgtgacaacg ccctgagcaa agcgcgtttc gagttccgct ggcgcgacca
     4321 attccgcctc agcctcgacc ctgaacgtgc cgagagcttc cacgacgaaa ccctgcctgc
     4381 cgaaggcgcg aaaatcgccc acttctgctc aatgtgcggc cccaaattct gctcgatgaa
     4441 aatcacgcag gaagtgcgcg actacgccga caagcaaaaa gcccaacggc agggcatgga
     4501 ggaaaaagcg gtcgagttcg tcaaaaaagg cgcgaagatt tacagttgaa acgtcaagca
     4561 aaaaatgccg tctgaaaccc ggaaaaaagg cttcagacgg tattctttcg cttgtg
//
LOCUS       DAWHRS010000148         4577 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWHRS010000148 DAWHRS010000000
VERSION     DAWHRS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.148"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(279..386)
                     /locus_tag="WIB82_001801"
                     /pseudo
     CDS             complement(279..386)
                     /locus_tag="WIB82_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(432..1160)
                     /gene="pdxJ"
                     /locus_tag="WIB82_001802"
     CDS             complement(432..1160)
                     /gene="pdxJ"
                     /locus_tag="WIB82_001802"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ3843062.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1184..1975)
                     /gene="recO"
                     /locus_tag="WIB82_001803"
     CDS             complement(1184..1975)
                     /gene="recO"
                     /locus_tag="WIB82_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ3843063.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLWVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSVSWYGSQELKTLHRAEWVGGWRQPQGRALFGGLYVNELVLK
                     LTAREDPVPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(2012..3139)
                     /gene="pheA"
                     /locus_tag="WIB82_001804"
     CDS             complement(2012..3139)
                     /gene="pheA"
                     /locus_tag="WIB82_001804"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ3843064.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEIVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3178..4410)
                     /locus_tag="WIB82_001805"
     CDS             complement(3178..4410)
                     /locus_tag="WIB82_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ3843065.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFVSSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         1021 a         1306 c         1263 g          987 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat tacataatca tcgcctcaat
       61 atttaatatc ccaaaatata gatttaaaac catcgtcttt gtggtttgtc agcattccta
      121 attgagatag aaagtcaaat aatttcttgt ttctccggct gtcatcaaga tggtttagat
      181 taagaccgcg aacaatacaa tacccaatac tctgtactgt attgtgaatt tcttctatca
      241 aactattatt gtttgcagat aaaatatact tttgattcat atagtgaatt aaatttaaac
      301 cagtacggcg ttggcttgcc ttgccgtact atttgtactg tctgcggctt cgccgccttg
      361 tcctgattta aatttaatcc actataacat atccctgtcg tgaaatgctg tctgaaaggg
      421 tttgcctgcc cttacggcag cagccttgcc ctgaacatcg cctccttcat ttggcgcacg
      481 gcttcgggca gtccgaggaa gagggcttgg gcaatcagcg aatgcccgat gttcagttcg
      541 cggatggcga ggatttgggc gatgggggta acgttgtgta tggtcagtcc gtgtccggcg
      601 ttgacgacca agcccaaatc gccggcaaaa tgcgcgccgt tttgcagacg ctcgaattgc
      661 ttgagttgtt cggcgtggct gcgcgcgtcg gcatacgcgc ctgtgtgcag ctcgacaacg
      721 ggcgcgccga catcataggc ggcttggatt tgcctgtcgt cggcatcgat aaagagagag
      781 actcggatgc ctgcgtcggt caggattttg gtgaacccgg cgattttttc ctgttgcgcc
      841 aatacgtcca agccgccttc ggtcgtgatt tcctgacgtt tttcaggcac gatgcacacg
      901 tcttccggca tcactttaag cgcgttttcg agcatttctt ccgtcaacgc catttcaagg
      961 ttcaggcgcg tgcggatggc gtttttgacg gcaaacacgt ccgcgtcttt gatgtggcgg
     1021 cggtcttcgc gcaggtgcat ggtaatcaaa tccgcaccgt gcgtttcggc aaccagtgcc
     1081 gcctccacgg ggctgggata agtcgtaccg cgcgcattgc ggacggtggc gatgtggtcg
     1141 atgttgacac ctaaaagcat aatctttcct tttatttctg ccttcagacg gcatttgaag
     1201 ccgtgccgtc cgaagtcggg acggtttccc gggcggtttc tttgcggtca aactgccgta
     1261 tctgttccaa cacctgccgc gatttcagcc cctcgggcaa caggtggcgg ataaaaagcc
     1321 gtgtgatttt caatgcctgt tgcaggcttt cggcagtgcg gaaactgcct tcgcgcaaat
     1381 cgatcaggct ctgcccgggg gcgacaacgc cggcggcgtg cggcggtacg gcaaatcctt
     1441 ttccgacggg gaagacggct gtttccgggc ggacaaggta tgtgccgcct gccgcaatcg
     1501 tcccgccgtc cccgtcgcgg ttcaaatcgg gggcaacgcc caacaggttc agcagccgcc
     1561 actcgaaacg gcgcaagtcg tcgatataag cggctttgca gcacaccgcc tccatcactt
     1621 ccgccaacgc gtcgtataac tcgggcaccg ggtcttcgcg ggcggtcagt ttcaacacca
     1681 actcgttcac atacaatccg ccgaacaacg ccctgccctg aggctgccgc caaccgccga
     1741 cccattcggc gcggtgtagg gttttgagtt cctgactgcc gtaccacgac acgctgacgg
     1801 gcacgaacgg caccaatacg ccgcgcagct cgctctgcct tttgcgcgcg ctgcgcgcca
     1861 gcaaagccac acgcccgtaa cggcggctga atgcttcaac ccacaggctg ctttcgcgcc
     1921 agggcgaaga tgccagcata aaaacgggtt catggttgac tcggtattcg gacataatcg
     1981 ttggggaaat gccgtctgaa cgctgccgcc gctacaaaac ggcggtcggg tacgaaccga
     2041 tgactttgac gaacgaagcg cgttcgccca agcgttccaa tgccgtctga atctgcgcgt
     2101 cccggcggtg tccttcgatg tcgatgaaga acaggtattc ccacaaaacg gatttgctcg
     2161 gacggctctc aaacttggtc atggaaatac ccgattcggt cagcggttgc agcagcgagg
     2221 caaccgcgcc tgcccggttg ggcgcggaaa cggccagcga agtcttgtcg ctgccgcttg
     2281 caccggtttc gtgatgtccc atcaccaaga agcgcgtggt gttgttcggt tcgtcttcga
     2341 tgcactcggc aaccatatcg agtccgtaga tttccgccgc cgtgcgtccg gcgatggcgg
     2401 caaccgtacc gtcgtccgat tcggcaacca gccttgcggc ttcggcattg ctggacacgg
     2461 caatccgttc ggcgttgggc aggtgtctgc ccaaccagtc gttgcactgc gccaacgcct
     2521 gcgcgtggga aaagactttg gcaatgcctt cggtgctgcc gttgttttta cgcaaaaggt
     2581 tgtggtggat gcgcaaaacg atttcgccgc acgcctgcaa cgcggtaacg gcaagcaggt
     2641 ctaacgtgcg accgaccgag ccttcggtcg aattttccac gggggcgacc agataatccg
     2701 cctgacgcgt ttcaacctgc ttgaagcagt cgtctatggt cggacacgcc atggtgtgcg
     2761 cggcgtgtcc gaaatgtttg attgccgcct gctgggtaaa cgtgccctgc ggccccagat
     2821 aggcgatggt cagcgggcgt tcgacggcga ggcactcgct catcacttcc cgaaacaggc
     2881 gtgctaccga ttcgtcgggc agcgggcctt tgttcaaatc ctgaatgcgg cgcaacacgg
     2941 cgacttcgcg ttcggggcgg tacactgcgc ccgtgccttt cagctcgccg atggcgtggg
     3001 cgtgttgcgc acgttcgttg agcaggcgca ggatttcggc atcgatggtg tcgatggcgt
     3061 tgcggtgggg aaggaggagt tcgtcgatag tttgggacat aatttcgcct gaacagtaat
     3121 ttatcgtgca ttctaacatc aaatgccgtc tgaaacggtt tcagacggca ttttccgtta
     3181 aaggtattcg ctttgcccgt tttctttcca cgcacgatgc gagcagagcc acagaagcgc
     3241 aatgccgcaa gaggtggacg cggtcatcgt tgccgccatc acggttgccg aaccgtcgtg
     3301 caagaaggtc gccgccatac ccacccccgc gccgattaaa gattggaata cacccaatac
     3361 ggcgtttgcg ctgccgccct cttctttgaa ataggacata aaacacgcct gcgtgtttgc
     3421 accgaccaag ccctgcgtac cgacggaaaa catcacgcac gcgaccagca gccaaaacgg
     3481 gggcaacccg aaaaacagca cggcggcgag ttgggacagg ttggcggcaa actggacgac
     3541 aatcccccac agcaggatgc tttgcggatg cacgccggtt ttgagccgcc acgcggtaac
     3601 gcggttgaaa aacatcatcg tgatgatgtt gagtgcaaac gcccaagcgt attgatgagg
     3661 cgtaacacgg tagagctgct ggtacacgaa ggaagattcg gtcagaaagg cgaacatcga
     3721 accgaagctg aatgcctgaa aaaacagata acccatcgca gcacgggttt tcaatacgcg
     3781 cttgaaccgc cccgccacca gcccgaacac gtcccgtccg attttgccgc cgacggcggg
     3841 cttgggcagg aaatactgta ccaaaccgag cagcaccagc gaatacgccg ccagaaaaac
     3901 aaaaatcgcc tgccagccac ccaagccctg caacaatgcg ccgaccatgg gtgcgaccag
     3961 cggcacaacc atcaaaatga tgccgataag ggcaaacatc tgggcggctt tgcgtccgga
     4021 ataataatcg cgcaccattg cgccgacgat gaccacagtc atgcccgcac cgaatgcctg
     4081 cacgacgcgc aggttgagga gctgttcggc actcgaaaca aatacgatgg cggcaacggc
     4141 aaggcaatat acaatcaaac cggtcagggc gacgggtttg cgccctttga tgtcggacac
     4201 cgaaccgccg accacctgtc cgaacgccgt gccgaacata aacaaactca aactctgttc
     4261 gatgcggtga acatccgcgt tcagcgattg cgccatttcg ggaatcgcgg gcaggtaggc
     4321 atcgatggaa aacggcatca gcgtaaccag catcgccatc aaaaccgcca tcagtttttc
     4381 gctcatttca ggataatggg cagaaggcat aaattctcgt ccgattcgca aacaaaccgc
     4441 ccggcggcgg aaaacacaat acgcggaaac ggaaaatttt aacacaccgg cacaaacagg
     4501 cggcaacaaa agccccggca aaaccggacg aaccggaacg atatagtgga ttaacaaaaa
     4561 ccagtacagc gttgcct
//
LOCUS       DAWHRS010000149         4570 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWHRS010000149 DAWHRS010000000
VERSION     DAWHRS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.149"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(17..93)
                     /locus_tag="WIB82_001806"
     tRNA            complement(17..93)
                     /locus_tag="WIB82_001806"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Asp,seq:gtc)
     gene            complement(126..201)
                     /locus_tag="WIB82_001807"
     tRNA            complement(126..201)
                     /locus_tag="WIB82_001807"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Val,seq:tac)
     gene            complement(271..2247)
                     /locus_tag="WIB82_001808"
     CDS             complement(271..2247)
                     /locus_tag="WIB82_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ3843066.1"
                     /translation="MNEFSLAPIVIVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGITMLSVM
                     GILMLTGVPFNWAFSVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2396..3235
                     /locus_tag="WIB82_001809"
     CDS             2396..3235
                     /locus_tag="WIB82_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ3843067.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3435..4466
                     /gene="gap"
                     /locus_tag="WIB82_001810"
     CDS             3435..4466
                     /gene="gap"
                     /locus_tag="WIB82_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ3843068.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGKLDGLAIRVPTINVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1161 a         1521 c         1066 g          822 t
ORIGIN      
        1 tcagtatttt gaaacttggc gcggcggacg gggctcgaac ccgcgacccc cggcgtgaca
       61 ggccggtact ctaaccaact gagctaccac cgcgcatcca ttgtttgcaa caatgaaaga
      121 aaacttggtg ggtgatgacg gagtcgaacc gccgacattc tgcttgtaag gcagacgctc
      181 taccaactga gctaatcacc cttgcgattt gcgaaagcgt tattaaacca aataaccgga
      241 aatggcgcaa gccttatttc ggcatttcat ttatattccc tgcaaactcc agttttcaaa
      301 agagataatt tcttcttttt tgcctgcgac caccaacacg tcaccgcctt ccaatgtaaa
      361 cgaggcatcc gggttttcaa tccggccggt gcggcggcgg acgaacaaga gtttgatgcc
      421 gtaagccgcc atcggaagcg tgccgactgt tttgccgacg gcgtatgctt ctgcagccaa
      481 aggaaaggcg tgacggacgg tttcgccgtt ttcgccgaat cctgcctcat catcactacc
      541 gacaaacagt ccctccaacg cggcataacg gctgtggcgg atatttgcca tcgtctgata
      601 gacgtgccga tacgacgcgc cgttgcctaa cattgcatag cctgccagca cgagtccggt
      661 ttctttggtg tccgacacgg cttcttccgc acctatatcg gtaaacgttt tcacataatc
      721 gtcgttggtg gcgcgcacat atacgggcat attgggatac atggacagca cattgtctaa
      781 aacgtgttgc gtttcgtgca tattgttgag cgtaaccacc accattttcg cccgtcccag
      841 accggcggct tccaatactt ccctgcgttt cgcatcgccg aacgacaccg gttcgcccgc
      901 acttctggca acctgcaccc gcgcaatgtc caagtcgagc gcgaaatacg gaatatcctc
      961 ttgggcaagg acgcgtccga ccgtctgccc gcccctgccg aagccgacaa tcagcacatg
     1021 gtcggacttg ctcatggttt ctaccagcat actgtgcaga tcgagcgact tcatgtccca
     1081 gcttgacttg accaaacgcc cgaccagcgc atcgctgccg cccaagagga agggcgcgat
     1141 aatcatcgac agcagaaccg ccgccgtcgc cgcctgttcc cattctggcg aaaccatatc
     1201 aagctgcccg gcaatggcca gcatcacgaa gccgaactcg ccgccctgcg cgagatacaa
     1261 agccgttttg aggctgtcgc cgaccgaatg tttcattttg aaggcaatgg caaacacaac
     1321 cagtgccttc aacaccagca gcattgccaa cagcatcaat acctgccgcc agccgccgat
     1381 caatgcctga atgtccagct tcatgccgac cgtgataaag aaaaagccga gcaaaatatc
     1441 gcggaacggg cggatgtcgt cttcgacttg gaaacggtat tccgtttccg aaagcagcat
     1501 gccggcaacg aatgcgccca acgccataga caaaccttcc agctcagtca gataagccac
     1561 acccaaggtt accagcagca cattgatcat aaagagttcg gacgatttgc gttttgccac
     1621 catcctgaac catcgcgaca taattttgct gccgacgaaa aacagcagcc ccagcgtcag
     1681 cagcattttt gcaaacgcca aacccaaggc cgcccaaata tttccgtccc ctccgcccgc
     1741 cagcgcggga atcagaatca tcagcggcac gacggcgatg tcctgcatca gcagcacgcc
     1801 catcgccatc tgaccgtgcg gctgccccaa ttccgtcttt tccgacaaaa tccggctcac
     1861 aatcgccgtg gacgacatcg ccaacgcgcc cgacacggaa aacgcccaat tgaacggcac
     1921 gcccgtcagc atcagtatgc ccattaccga cagcatcgta atgccgacct gcaaaccgcc
     1981 cagaccgaac accagccgcc tcatcgccct caacttgggc agcgagaact ccaaaccgat
     2041 gctgaacatc aggaacacaa tcccgatttc gcccaaataa tccgtcgcat ggcttttcgg
     2101 aatcaggctg agcataccgg gccccgccaa aaagcccacc agcaggtagc ccagcatgga
     2161 gggaatgttg aacttgcggc acaggatcac cgtaatgacc gacaccagca aaacaatcac
     2221 aataggggca agcgaaaatt cgttcataga ccgtccgaac aggaaaatac agaaaaatgc
     2281 cgtctgaaac ggcatacgcc gccgtattat aacaaaacac cgcgcaccat ccgaaacggg
     2341 cgcggcatac aattctgcta aaatacgccc tttcgatttt gagccgcaca ccgacatgac
     2401 cgccacagcc gccgccatag accgcctgct tccccaaacc caatgccgcg aatgcggcta
     2461 cgacggctgc ctgccctacg cacaggcagt cgcaacaggc gaagcgtaca acctctgcgc
     2521 cccgggcgga gaagccgtca ttcgggacat ttccgccctg ctcggcaaac cccttgtcgc
     2581 acctgccaaa acccaagcca aagcgctcgc ccggatagac gaaaccgcct gtatcggctg
     2641 caccgcctgc atccgcgcct gccctgccga tgccattatg ggtgcgggca aacttatgca
     2701 caccgtcatc gccgacgaat gcaccggctg cggactctgc gtcgccccct gccccgtcga
     2761 ctgcatccat atgcagcccg ttgccgacac cgtcctgccc cgcgcgcgcc gcttcagcct
     2821 gtccgccgac agccgttttg ccgccgccga acacgcgcgt gcgcgctacc tcaaacgcaa
     2881 cgaacgcaaa cagcgcgaag ccgacgaacg caaggccatg cttgccgaac gcgaagccgc
     2941 cgtccgcaac gcgcgtccgc aaacacccga cacaccgaaa aaaccggcgt ttaaccctgc
     3001 cgacctcatc gccaaagcca tggcaaaagc gcaaacccaa caagaccgcc tcgccgccgc
     3061 cgacaaccgc aaagactatc aggcgaaaca gatagccgaa gcccgcgaac gcgccgagtt
     3121 gcgccgcgcc caacgcgata tgaaatacgg cagcgacagc gaaaaagccg ccgccctcga
     3181 atacctcaaa caatacaaag ccaaacagga agccgcacag aataccgcct cctgaccctt
     3241 ccctgatatg ccgtctgaag ccgcttcaga cggcatttta tcaagctctc cgtccgccgc
     3301 acccgtgccg tccgcatctt accgcccacc cttccggccg cgccgttttc aataaaatat
     3361 taattacacg ccactacaaa tttgctataa tccgccccga aaatctaccc aacccctcaa
     3421 caaaggaacg aaccatgggc atcaaagtcg ccatcaacgg ctacggacgc atcggccgcc
     3481 aggttttgcg cgccatctac gattatcaga ttcaagacca actccaaatc gtcgccgtca
     3541 acgccagcgg cagccttgaa accaacgccc atctgaccaa attcgacacc gtacacggac
     3601 gctttgaagc cgacgtatcc cacgacggcg gcaacctcat cgtcaacggc gacaaaatcc
     3661 ccttcttctc gacccgcaac cctgccgaac tgccgtggaa agaactcggt gtcgatttgg
     3721 tcatggaatg caccggcgcg ttcaccagca aagaaaaagc caaagttcat ctggaaagcg
     3781 gcgcaaaaaa agtcctcatt tccgcaccgg gcggcgacga tgtcgatgca accgtcgtgt
     3841 acggcgtgaa cgacagcgtc ctgaccgccg acatgaccgt cgtttccaac gcttcctgca
     3901 ccaccaactg cctctcgccg gttgccaaag tgttgagcga aagcgtcggc atcgtcaaag
     3961 gcgcgatgac caccatccac gcgctgacca acgaccaaac cgttaccgac gtgcgccaca
     4021 aagacctgcg ccgcgcccgc agcggcgtgg aaaacatgat tccgaccaaa accggcgcgg
     4081 caaaagccgt cggactggta ctgcccgaat tgaaaggcaa actcgacgga ctcgccatcc
     4141 gcgtgccgac catcaacgta tcattggtag atttgagctt ccaagccgcg cgcgacacca
     4201 cagtcgaaga aatcaacgca ctgatgaaag ccgcctcgga agcgggtgcg ctcaaaggcg
     4261 ttttaggcta caacaccctg ccgcttgttt ctatggactt caaccacacc accgaagcca
     4321 gccacttcga cgccacactg accaaagtcg ttgacggcaa catgattaaa gtcttcgcat
     4381 ggtatgacaa cgaatggggc ttcagctgcc aaatgctgaa caccgcacgc cgcatgttcg
     4441 gacttgaagt gcgcccgctc aaataagcaa caaaccgtca aacaaaatgc cgtctgaaac
     4501 ccgatgtttt caagtttcag acggcatttt tcaccgtgat tttacccggc tgtcgtcatt
     4561 tccaatttta
//
LOCUS       DAWHRS010000150         4446 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWHRS010000150 DAWHRS010000000
VERSION     DAWHRS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.150"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(64..1206)
                     /locus_tag="WIB82_001811"
     CDS             complement(64..1206)
                     /locus_tag="WIB82_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ3843069.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLHLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLLAGYDGTQPFQDPFCGSGTIAVEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWTDLRRRAEAQIRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTSDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVEGSNR"
     gene            complement(1255..1677)
                     /locus_tag="WIB82_001812"
     CDS             complement(1255..1677)
                     /locus_tag="WIB82_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843070.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1670..2479)
                     /locus_tag="WIB82_001813"
     CDS             complement(1670..2479)
                     /locus_tag="WIB82_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ3843071.1"
                     /translation="MIQDTRPLIRVVAGILLNRDGDYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHAHVCLKFLWVNPDQWTGEPQ
                     SREGQAWSWQKAGDFTIAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAEESGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2561..2938)
                     /locus_tag="WIB82_001814"
     CDS             complement(2561..2938)
                     /locus_tag="WIB82_001814"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ3843072.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3138..3905)
                     /locus_tag="WIB82_001815"
     CDS             complement(3138..3905)
                     /locus_tag="WIB82_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ3843073.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3915..>4446)
                     /locus_tag="WIB82_001816"
     CDS             complement(3915..>4446)
                     /locus_tag="WIB82_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ3843074.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPTSFDKKQGVIWGNILSLNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1088 a         1101 c         1091 g         1166 t
ORIGIN      
        1 atcggccgga tattaaaaaa agccgtctga acatttttca gacggctttt ttgtacactt
       61 tcctcaacgg ttcgatcctt ccaccatatc aatcaggaac aggcggcaat cgaggttgcc
      121 gttataaagc gggattttgc gttttggcga caggcgcatg aatttgggca tttccctgtc
      181 gctggtaaac atgccgaccc gccagccggc atagtgtttt ttcaaccacg tccccagctg
      241 cggataaaga gcctgcaaag cgcggacttc ctcaaggcgc acaccgtagg gcggattgga
      301 caccatgatg ccgttttcgc cgttcggacg gacggattgc gcgtcggcaa cgctgaagga
      361 aacgatgtcg tccacttcgg cactgcgtgc gttgtccaat gccgtctgaa cgatgcggcg
      421 gtcgctgtcg ctgcctgcaa tcggggcgcg gacggggcgg atttgcgctt cggcgcggcg
      481 gcgcaaatct gtccacagcg ttttatcgaa attttgcagt ttctcaaaac cgaaacggcg
      541 catcataccc ggcgcgcggc ctgtggcaat ccaagcggcc tcgacggcaa tcgtgccgct
      601 gccgcaaaac gggtcttgaa acggctgcgt gccgtcgtag cctgccaaga gcagcagtcc
      661 ggcagcaagg ttttcgcgca gcggggcttc gccggtatcc aggcggtagc cgcgtttaaa
      721 cagggcttcg cccgaagtgt cgataaagat ttcgacattg cgttcgttca aaaaggcgtg
      781 gatgcggaca tcgggcgcgg ctttgtccac gctcggacgt gcgtcgtaaa tgtcgcggaa
      841 agcgtcgcag acggcatctt tgacggtcag tccgacaaat tggatgctct taacgttggc
      901 acgctttgcc tcgactttga ctttgaacgt ctgctgcaag gtaaaccagt tgaaccagtt
      961 gatgtttttg gcaagtttgt aaatgtcgcg ctcggttcgg tatgtgcctt tggtcaggtg
     1021 cagcaggata cggccggcgg tgcgcgagtg caggttggcg gcgtaggcct gttccaatcc
     1081 gccgcggcag gaaacgccgc cgtcaaacgc ttgaacatcg gtacagccga ggcttccgag
     1141 ttcttgagat aaaacggttt ccaagccgcg cgggcaggtg gcgaaaagtg tatataaagt
     1201 attcataacg tgtcctttcc ggacggatgt tccggcaatg gcggattgaa aggtttattc
     1261 ggcggtaaat gccgtctgaa atgtccggct gccgattgta atgtctgccg taaaatcgcg
     1321 cctgccttcg acacagatgg gcaggcggat gcgtactgcc tgccaagtcc ccgacggttg
     1381 ccgctcgaac atatagcggt tgaaacccat atccatattt ttcatactga agctgatgct
     1441 gacctgttcc gtgccgacgg gcgcgtgttc gatataaata tcaaacggtt ttttggttga
     1501 aacggcggcg gcgcggacgc ggcttccgtc cggcagcgtg caaccctcgg tcaaatcgca
     1561 ttgcgccgcc acggcttgcg gctgctgcgc ctgccaccat tgcaacaaaa cgagcttgac
     1621 ggcggcaaag gcaatcagca gcagtgcggc aagcagtaat ttacggtttt tattcatgat
     1681 tcttccctat tgtttcatcc cttaccacca cgtgcgcccc caatccgagc catagttttc
     1741 cataacgtgc aaccgtctgt ccgtttgctg caagtacgag cggcacggat acgccctgcc
     1801 gcagggcttc tgccgcctct tgtgcctgaa catcatcgca cacgcgccaa acgaccgcat
     1861 ccgcgccctt ttcctgcgcc tgccgccatt gttcgcgggt ctgcaccatc atccacacgc
     1921 tgtcggcgtg ttcgggtctg tcctgccatt gcgccgcctc catcaaaacg ttcgcaccgc
     1981 tttcctctgc cgaacccaaa ggcaggacgc ggtacgcgcc catactgttt tctccgtaca
     2041 aacctgtttt caggctgccg tacaaacggc gcggaacgga caacgaacgc aaaagcgcgc
     2101 cgttggcggg cagcatgggg gcaatggtaa aatcacccgc cttctgccac gaccatgcct
     2161 gtccttcgcg cgattgcggt tcgcccgtcc attggtcggg gtttacccat aggaatttca
     2221 ggcagacatg cgcgtgttcg taggaatgga ttttggtcag ccaaggcgtg gcggcgagga
     2281 tgcggatgcc gagttcttct tcaaactcgc gttgcaaggc ttggaagtcg gtttcgcccg
     2341 cttcgacctt gccgccggca aattcccaat atccggcata gggtttacct tcggggcggg
     2401 aactgagcag gtagtcgccg tctcggttga gcaggatgcc ggcaacgacg cggataaggg
     2461 gtcgggtgtc ttgaatcatg gcgggtacgc ggttggtcgg tcggagtgca aatgagggac
     2521 gggcaatgcc gtcaacgggt ttgccgcatt tcagacggca ttatttttcg gcaacgacaa
     2581 acgccaatac ggtgtcttct tcgtcgctca tgctgaggct gacgcggctg atgccttgtt
     2641 cctccagcca tttggacagg gcggggccgt agaaaaattc gggcttgccc aatgcgtcat
     2701 gcccgatgcc gatgttgcgg aaggaaaccg cgccgcgtat gcccgtgccg acggctttgg
     2761 caaaggcttc tttggcggca aagcgtttgg caaggtagtt gacgggtttg cccgcctgag
     2821 gaaattcaag cagctcttcc ggagtgagga tgcgcccggc aaacgcctgt ccgaattttt
     2881 tgtttaagcg gatgatgcgc ttgagggaaa caatgtctgt gccgatgccg taaatcatat
     2941 ttgcgctcct tcgcccttgg ttgcgggtag tgatgaatgt gatgatgaat gccgtcaatt
     3001 tcttggggcg ttaaaatggc gtgaatatcc actttcgggg ttttgtcggt aacaattttt
     3061 attgtaacct tttgcatttg aaattctaaa acatcgtgcc aattatttaa taaggagtta
     3121 aggcatttaa taatgaatta aagtttgatt aaacccgaat gcccgatttt cagagccaat
     3181 accaataaaa caataaaaat taacatttta aaaaaatttg ggctaatctt agtccttaac
     3241 cgaattccaa catacaatcc aataacagac aatacggaca gtaaaaatat taaaccgtat
     3301 tcactcttat ttaataacca atactggtct cttagcatat atatttgaac aattttcgcc
     3361 aaaagatagc atagattgct tgattttacg atacgatttt tattttctgt ttcgctaagc
     3421 aaaaatatta acaatatggg agacatggca ttggttgaac cgccgatgat gcctgccaaa
     3481 aacccaaaaa gaaccatatt cttattattg gcaactactt gaatattttt tgcttttgca
     3541 catacattta aaataccatt gacagaataa tacaatgtaa tgattgccat cagtaaaagc
     3601 agccaagaca ctggaagtat caaaagcaac ttcaccccca aaatgctgcc aacgacgctg
     3661 ccgatagcaa gcaatttata ggtttttaaa taataaacaa tctcttgcca aaaacccttt
     3721 ttgttattgc tgcatagaac caacaagctc attaacaggc ttggtaatgc caccaaggca
     3781 acaaccttag acaatggcat gataaaagcc aatgcggttg taccgagcat cggaaatccc
     3841 atgcctgtaa ttccgtgcag tattgcggca gcaacaaaaa cgatagattg cattatttct
     3901 tgcataaccg tcctttatac agtaattctt gctctaatat aatgcctgtt tttatccgta
     3961 taatcagttc tggcatgcaa gccattgaca ttgtcaataa taatcaaacc atcatcttgt
     4021 gcagaaaatt cttgaatatc actcgcattt tttataacat ttacaagata atcaagtgca
     4081 agtaccattt cctttgatac tttatttcta ttttcttcaa tacctttata gatacaatca
     4141 cttctaaatc taatcatttg tgtattgagc gataagatat taccccatat cacaccttgt
     4201 tttttatcaa atgatgttgg tgttttaaat ggatataaat tgcccgttaa tgtttttaag
     4261 tgttttttac cggtttctgt tttagataac tgattgacaa tatctgatga acttaaaaat
     4321 agggaattac ctccatcatt ggctgatttt actacataca taaccaaata actttccggg
     4381 ttttcactaa aagatgaatc actatgaagt ggacattctc caacatcttc cgagaaagtt
     4441 atatca
//
LOCUS       DAWHRS010000151         4213 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWHRS010000151 DAWHRS010000000
VERSION     DAWHRS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.151"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(84..1130)
                     /gene="recA"
                     /locus_tag="WIB82_001817"
     CDS             complement(84..1130)
                     /gene="recA"
                     /locus_tag="WIB82_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ3843075.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1384..2148
                     /locus_tag="WIB82_001818"
     CDS             1384..2148
                     /locus_tag="WIB82_001818"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ3843076.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGAFPCSDDYYFKLL
                     DALIESRLPDIIDIELFAGETAVRRAVADAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            2168..4183
                     /gene="rep"
                     /locus_tag="WIB82_001819"
     CDS             2168..4183
                     /gene="rep"
                     /locus_tag="WIB82_001819"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ3843077.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRHKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHKHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT          991 a         1156 c         1163 g          903 t
ORIGIN      
        1 caaaactcaa atcaagccgt tcggaggcgg ctcaaaaaaa cggtacttcg cagcagaagt
       61 accgtttatc gggatttcag gttttattct tcggggcgtt cgccgtcggt ttcgtcctgc
      121 gtcccttcgg tgatgtgcat ttctacgccg ttgagggcgc ggatttttgc gtcgatttca
      181 ttggcgactt cgggattttc cttcagccag acgcggacgt tgtctttgcc ctgaccgatt
      241 ttcgcgccgt tgtagctgta ccacgcgccg gatttgttga tgatgtcgtt tttcacgccg
      301 atgtcgatca attcgccttc ccaactgatg ccttctccgt agaggatgtc aaactctgcc
      361 tgacggaacg ggggggcgac tttgtttttg atgactttga cgcgggtttc gttgcccaat
      421 acttcttcgc cttttttgat ggatccggtg cggcggatgt cgaggcggac ggaagaatag
      481 aatttcagcg cgttgccgcc ggtggtggtc tcggggctgc cgaacattac gccgatcttc
      541 atccggattt ggttgatgaa cacaaccagc gtgttggttt ttttgatgtg tccggtcagt
      601 ttgcgcaaag cctgactcat caggcgcgcc tgcagtccga catggctgtc ccccatatcg
      661 ccttcgattt cggctttggg gacgagtgcg gctacggaat cgacgactac catatctatg
      721 ccgcccgaac ggacgagtgt gtcgcagatt tccaaagcct gttcgccggt atcgggctgg
      781 gacaggtaaa gctcttcgac ttttacgccg agtttgcggg cgtaaacggg atcaaaggcg
      841 tgttcggcat cgacaaaggc gcacacgccg ccgtttttct ggcattgggc gacggcttcg
      901 aggcagaggg tggttttgcc ggaggattcg gggccgaaga tttcgacgat gcgcccgcgc
      961 ggcagaccgc cgactccgag ggcgaggtct aatccgagcg atccggtgga aatgacttcg
     1021 aggttttctt cctgctggct gccgtccatt ttcatgatgg cgcctttgcc gaaacttttt
     1081 tcgatttgcg ccagtgcggc ggcaagggct ttgcttttgt cgtctgacat tggggttact
     1141 ccggaacaaa tgcggtatgt aggatgcggc gcaacacggg ctgcggcgcg ggatgtgtat
     1201 cgttttcccg atgtgcgggc tatcggtaat gctgcttcac gaggttgcca ttatcgcata
     1261 tttccttgct tgccgatatg cggcaggacg cggcggcttg tgccggaatg gaatctggat
     1321 gccgtctaaa aggcggccgg ctttgttata atggcggctg ttttttctgt gtgtgcctgt
     1381 tttatgtgtt cctgccttgt tgtcaaaaat accgttatcg gaagcggacg caccaaaatc
     1441 gccgtgccgc ttgtcgcccg cgatgccgcc gaactttccg ccgtacttga ccaaatcaaa
     1501 aacctgccct tcgacatcgt cgagttccgc gccgattttt tggaatgcgc gggcagcatc
     1561 ggcgaagtat tgcgccacac gcagaccgtc cgcgacgcgc tgcccgacaa gccgctgctg
     1621 tttacgttca gacggcatgg cgaaggcggc gcgttcccgt gttcggacga ttattatttc
     1681 aaactgctcg acgcgctgat cgaaagccgc ctgcccgaca tcatcgacat cgagctgttt
     1741 gccggcgaaa ccgccgtccg gcgcgccgtg gcggatgctc aaaaaaacgg catcgccgcc
     1801 ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag aagaaatcgt atgccgtctg
     1861 aaacagatgg aggactgcgg cgcggacatc tgcaaaattg cggtgatgcc gcaaagcgcg
     1921 gaagatgtgc tgactttgct ttccgccacg ctcgaagcga aacggcttgc cgccaaaccg
     1981 gttgttacga tgtcgatggg gcagacgggt gcggtcagcc gccttgccgg acaggtgttc
     2041 ggctcaagca tcacgttcgg ttcgggaacg caaaactccg cgccggggca aatcggcgta
     2101 tccgccctcc gtgcggcact cgactgtctc gaaagcggcg cagactgatt tcagacggca
     2161 tcaaaacatg atgaaactca atccccaaca gctcgaagcc gtccgttacc tcggcggtcc
     2221 cctgctcgtc ctcgccggcg ctggcagcgg caaaaccggc gtgattacgc aaaaaatcaa
     2281 gtatttgatt gtcaatgtcg gctatctgcc gcataccgtt gccgcgatta cctttaccaa
     2341 taaagccgcc gcggaaatgc aggagcgcgt cgccaaaatg ctgcccaagt cgcaaacgcg
     2401 cgggctgacg atttgcacgt tccattcttt gggcatgaag attttgcgcg aagaggcgaa
     2461 ccatatcggt tacaaaaaaa acttctccat tctcgattct accgacagcg cgaaaatcat
     2521 cggcgaactc ttaggcggta cgggcaaaga agccgtattc aaggcgcagc accagatttc
     2581 cttgtggaaa aacgatttaa aaacgcctga agatgtcgtt cagacggcat cgaacatttg
     2641 ggaacaacaa accgcacgcg tgtatgcgag ctatcaggaa accttacaaa gctatcaggc
     2701 agtggacttt gacgacttaa tccgcctgcc tgccgtgctg ttgcagcaaa acagcgaagt
     2761 gcgccacaaa tggcagcggc ggctgcgtta tctgctggtt gacgaatgcc aagatacgaa
     2821 tacctgccaa tttacgttga tgaaacttct gaccggcgcg gaaggtatgt ttaccgccgt
     2881 cggcgacgac gaccagtcca tctacgcatg gcgcggtgcg aacatggaaa acctgcgtaa
     2941 aatgcaggaa aactatccgc agatgaaggt catcaaactg gagcaaaact accgctccac
     3001 cgcgcggatt ctcaaaatcg ccaacaaagt catcgaaaac aaccccaagc tgtttaccaa
     3061 aaaactttgg tcgcaattgg gcgaaggcga gccggtcaaa gtcgttgcct gccaaaacga
     3121 gcaacacgaa gccgactggg tcgtcagcca aatcgtcaaa caaaaactca tcggcggcga
     3181 caaaacccaa tatgccgatt tcgccgtgtt gtaccgggga aagcatcagg cgaggatttt
     3241 cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc tccggcggac aaagcttttt
     3301 cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg ctgcttgcca accccaacga
     3361 cgatcccgcc tttctgcgtg ccgttaccac gcccaaacgc ggcatcggcg atgtaacgct
     3421 gggcaagctc aacacttacg cgcacaaaca cgaatgcagc ctgtatgaag ccgcgcaaaa
     3481 cgaagaagcc ctcgccacgc tgaacaatac caaccgccaa cacctgcaag cctttatgga
     3541 tatgttcggc agttaccgtg ccaaagccga aaccggcgaa gcgggcgagt tcatcaacag
     3601 cctgctcgaa gaaatcgact atgaaaacca cctgcttgcc agtgaagaag gcaaagctgg
     3661 cgaaatcaaa tggcgcaacg tcggcgattt ggtatcatgg tttgcgcgaa aaggcgggga
     3721 agacggcaaa aacatcatcg aactcgccca aaccgtcgcc ttgatgacgc ttttggaagg
     3781 aaaagacgaa gaagaaaccg atgccgtctc gctatccacg ctacacgccg ccaaaggttt
     3841 ggagtatccg tatgttttcc ttgtcggttg cgaagaaggc gttttgccgc acaacgacag
     3901 catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg tacgtcggca tcacccgcgc
     3961 caaacgccaa ctcacactga cccactgcgt caaacgcaaa aaacaaggca catggcagtt
     4021 ccccgaaccc agccgattca tagacgaaat gccgcaggaa gatttgaaaa tcctgggtcg
     4081 caaaggcggc gaaccgattg tcagcaaaga agaaggcaga cgcaaccttg ccgatataat
     4141 cggaaggctc gacagcatga aaaaaggcgg tccggaggat taaaccaaga tagggaaacg
     4201 aagccggaga caa
//
LOCUS       DAWHRS010000152         4206 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWHRS010000152 DAWHRS010000000
VERSION     DAWHRS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.152"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(1..143)
                     /locus_tag="WIB82_001820"
                     /pseudo
     CDS             complement(1..143)
                     /locus_tag="WIB82_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(271..1521)
                     /locus_tag="WIB82_001821"
     CDS             complement(271..1521)
                     /locus_tag="WIB82_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ3843078.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVRKQVTALCNKYPVYGA"
     gene            complement(1588..1783)
                     /locus_tag="WIB82_001822"
                     /pseudo
     CDS             complement(1588..1783)
                     /locus_tag="WIB82_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2002..3777)
                     /gene="ggt"
                     /locus_tag="WIB82_001823"
     CDS             complement(2002..3777)
                     /gene="ggt"
                     /locus_tag="WIB82_001823"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ3843079.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3945..4181)
                     /locus_tag="WIB82_001824"
     CDS             complement(3945..4181)
                     /locus_tag="WIB82_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ3843080.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIASRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
BASE COUNT         1043 a          989 c         1007 g         1167 t
ORIGIN      
        1 atctctgagg taacgggttt ttgacggttc aggtatggtt cgatcggctg ccaatcaatc
       61 acccggtcca acttcaatag cgggaaacgg tcgatgtgtt tggcaattat ggcttgtgcg
      121 gtttgccgga agaaggtgct catgagaaat cccctaaatg tcttggtggg aatttagggg
      181 attttgggga tttttgcaaa ggtttccgcc tgaaacatta tgagatttca ggcggcattg
      241 gattgcttgg cggaatattt ttaaaaaggc ttacgcgccg taaacggggt atttattgca
      301 caaagcagtt acttgtttgc ggactttggc gaggttggct tcgtcttcgg ggttagacaa
      361 tacgtcggca accaagttcg ccaatacgcg tgcgtcggct tcgttaaaac cgcgtgtggt
      421 catggcagcg gagccgatgc ggatgccgga ggtaacgaag ggtttttccg gatcgttcgg
      481 aatggcgttt ttgttgacgg tgatgtgcgc tttgcccaaa gcggcttcgg cggctttgcc
      541 ggtaattttc atcggttgca ggtcgacgag gaaaacgtgg ctttcggtgc ggccggaaac
      601 gatgcgcaaa ccgcgtttga ccagttcttc cgccatggcg gcagcattga ttttcacttg
      661 ttttgcgtat tgtttgaact cgggttgcaa cgcttcttta aacgctacgg ctttggcggc
      721 gatgacgtgc atcagcggac cgccttgcag gcttgggaag atggaagagt tcagtgcttt
      781 ttcgtgggta ttgtcgcggc acaaaattac gccgccgcga ggaccgcgta gggttttgtg
      841 ggtggtggtg gtcacgaagt cgcagaatgg cacggggttg ggatattcgc cgccggcaac
      901 cagaccggca tagtgcgcca tatcgacaaa gaggtatgcg ccgactttat cggcgatttc
      961 gcggaatttt gcccagtcga tttgcaacgc gtaggcagac gcacccgcca caatcatttt
     1021 gggtttgtgt tcgagagcca agcgttccac ttcggcataa tcgagcactt cgttttcatc
     1081 caaaccataa gtaacggcat tgtagagttt gccggaaata ttaacgctcg cgccgtgggt
     1141 caggtggccg ccgtgcgcca aagacatacc caaaatggtg tcgcccggtt ttaaaacgga
     1201 agcgtacacg gcttggttgg cttgcgagcc ggagtgcggt tgaacgttgg cgtattgcgc
     1261 gccgaacagt tttttgacgc ggtcgattgc caattgttcg acaatatcga cgtattcgca
     1321 gccgccgtag tagcgtttgc cggggtagcc ttcggcgtat ttgttggtca gctgggaacc
     1381 ttgcgcttcc attacggcgc agctgacgta gttttcagag gcaatcagct cgacgtggtc
     1441 ttgctggcgt tggtcttctt gggcaatggc tgctgccaaa tcggggtcat attgtgcgag
     1501 ggtaacgctt tttgaaaaca tgttctcggc tcctttgtgt aatcagggta tcatgagtgt
     1561 tttttgtata aaaaaatact tcaaaaccta aggcagatag tccataatgc gtaaattttc
     1621 tttggcatta tcaggtaatt tatttaacat gctggttttt agcgtctcat tacctttatg
     1681 ttagtgcaag gctacccaaa ggcaatgcat caaatggtaa attttcggca gtttgtgcaa
     1741 aatcaatcaa ataaccagca ccaaccacat ctttatcagc cataacccgc tgatacacca
     1801 cttgccaaga caactctcct gccccaccat gtcttaaaat cagatgaatg gcttgtaatg
     1861 ctgtgtttgg cgtctcacaa attgcgaaga tctgctcaga agcttgcttg atggcttgta
     1921 tttttgtttg attactcaca atttccccct attttaatat taacttaatg cggtcaaatt
     1981 cacaaaatac aagcttcacc tctaatcacc catcactcga ccttctcggc gtggatcggc
     2041 accaccaacc agcctgcttg gctcgataat aatggcttga acacctgaat ttagctcacg
     2101 cacgtcagtc ttatagccca aatcatttaa tgcttgttgc cactggaggg cggttgtacc
     2161 cgtttctagt tcatagctac caaagcgatt taataaattg ggtgcactga tggcatcttg
     2221 gatatccata ttccagtcac tatgtgccac aatcgtctta gcgacatagc caatgatacg
     2281 gctaccacct ggggagccga ttgccatata aggcttgcct gctttaaata cgatggttgg
     2341 tgccattgag gagcgtggtc tcttgccggg ctcgacacga ttggcgacct gtttgccctg
     2401 ctttattggc tcaaaactaa agtctgtcag ctcattattc agcaggtagc catttgccat
     2461 caaagttgag ccaaacgcat tttcaatgga agtcgtcatt gatagcacat tgcccgcctt
     2521 atccacaatt gatatatgac tggtagaagg taactcaatc gcttgtgagg acacccactc
     2581 atgaataaaa tcgcctgcag atacgctagg caatgcctta tccgactgct caagcagctg
     2641 gctgcgatgt tttaggtagt ctttagaaat caactggcga atgggtactg gtacaaaatc
     2701 agggtcgccc aaatatacat cacgatccgc aaacgcaagc ctagaagcgt cgcccaagag
     2761 acgtaaacct tcagcatcat accccacctg attgggtgaa aattcattta aaatccccaa
     2821 aatctgaccc acagcaatcc cacctgagct tggtgcaccc ataccgcata cttcataaat
     2881 acgataagtc acacaaacag gcgggcgttc caccacttga taatcagata aatcttgtaa
     2941 ggataattga ccggggttat ccttagcatt ttggacaact gaaacgatat tttgggcata
     3001 tttaccagta tgcagagctt ttgcaccttg agctgctaac gcctgaacac tgtcagcaaa
     3061 ttctaaattt ttcagcaagc tgcctgcttg tagcggcaca ccattcggca aaaaataagc
     3121 ggctgttttt ggatagcgtg ccaaatgctg ctgattttgc tcaaccgaga tggcaagcct
     3181 tggcgacacc tcaaagcctt gttttgccaa gcggatcggt gtatcaaata attttcccca
     3241 aggcaataca ccgtatcgct gatgtattgt ctccatcagt ttagggatag caggcgtacc
     3301 caccgagcga ccaccgacca ccgcttccat aaatttcaat ggttgaccat ctttatccaa
     3361 aaataattcc ggcgtcgcac gcatcggtgc cgtctcacgc ccatcaaatg tggtcaatgt
     3421 tttggcggta ttatcccaat acaacacaaa tgcaccaccg cccaagcctg acgactgtgg
     3481 ctctaccaag cttagtgtcg tctgcaccgc caccatcgca tctgcagcgc taccgccttg
     3541 ctttaagata tcatagccag cttgtgttgc taatggattg gctgacgcta ccataaaatc
     3601 acttgcaatc acctgctttt gttcggtcag tcccgttgca tgttcaggcg tgtgagcgtc
     3661 tgcacctgtg atgacagcag aatgagtatt aaccttacct tgattggcat ggatgacttg
     3721 acatccagag attgtcatag acattatcaa tgcagtcaat aaatatgttt tagccacaag
     3781 cactccttcg cctgagtttg attgatgatt catacaaggc atgctgatta ttgtatttaa
     3841 tatggctaaa taattcaatc caaactatca atcttgacca tcaaaaaaag accgctaatg
     3901 tcatcagcag tcttttttga tatttatttt aagatattaa gtaatcagac ctttgggcta
     3961 tgctcttcaa tgagtggttt tagctcacct gattggtaca tttgtaggat aatatcacta
     4021 ccaccgatta actcaccatt aacccaaagc tgtggaaagg ttggccgact ggcgatgagt
     4081 ggtagagtac tgcgaatttc tgggttttct aggatattaa caaaagcaaa gggtctgcca
     4141 attgggtcag cacctctact gcacgcgctg aaaatccaca ttggggaaac tggggcgtgc
     4201 ctttca
//
LOCUS       DAWHRS010000153         4180 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWHRS010000153 DAWHRS010000000
VERSION     DAWHRS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.153"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            329..1450
                     /gene="dnaJ"
                     /locus_tag="WIB82_001825"
     CDS             329..1450
                     /gene="dnaJ"
                     /locus_tag="WIB82_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ3843081.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGTHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1646..3664
                     /locus_tag="WIB82_001826"
     CDS             1646..3664
                     /locus_tag="WIB82_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ3843082.1"
                     /translation="MNKSLSSSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(3708..>4180)
                     /gene="rfbC"
                     /locus_tag="WIB82_001827"
     CDS             complement(3708..>4180)
                     /gene="rfbC"
                     /locus_tag="WIB82_001827"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ3843083.1"
                     /translation="FRDGWFKTQVCERTFVQENHSKSGKGVLRGLHYQTENTQGKLVR
                     VVVGEVFDVAVDMRKDSPTFGKWAGATLSAQNQCQLWIPEGFAHGFYVLSDTTELTYK
                     CTDYYKPEAEQVLIWNDPAIGIGWPLQTAPLLSPKDLAGKAWAQAEKLRLTLSR"
BASE COUNT          886 a          962 c         1238 g         1094 t
ORIGIN      
        1 gtcggcgcgc ccgcaccaaa agccccagcc aaaccggtgc tgcccgcgga taaaaaataa
       61 aataagggga agtctgcagc cgcatcaaat gccgtctgaa catgcgttcg ggcggcgttt
      121 ttataacaaa aacacttcat ggcggttggt tttatgcctg tctaagtttt tgtgtcgtgc
      181 atacctgaag atttcagacg gcatcggttt atgctgtctg aaaagtgtat tccgtttcag
      241 tttgtaagct atggcagtct gtttgtcttg tgttttgcgc aattgccctt attttgagcc
      301 gtgattttat tttgaattag atgaaaaaat gagtaatcaa gatttttatg cgacgctggg
      361 tgtggcaaga acagctaccg atgatgagat taaaaaagcc taccggaaat tggcgatgaa
      421 ataccatccc gaccgcaatc ctgacaataa agaggcggaa gagaagttta aagaagtaca
      481 aaaggcgtat gaaactttgt ccgacaagga aaagcgcgct atgtacgacc agtatggtca
      541 tgcggcgttt gaaggcggcg gacagggggg cttcggaggg tttggcggat ttggcggtgc
      601 gcagggtttt gactttgggg atattttcag ccaaatgttt ggaggcggtt cggggcgcgc
      661 ccagcctgat tatcagggtg aggacgttca agtcggtatc gaaatcacgc ttgaagaagc
      721 cgcaaaaggt gtgaagaaac gcatcaatat tccgacttat gaagcgtgtg atgtctgtaa
      781 cggcagtggc gcgaaaccgg ggacatcccc ggaaacctgc ccgacttgca aaggttcggg
      841 tacggtgcac atccagcagg cgattttccg tatgcagcag acttgtccga cctgccacgg
      901 tgcgggcaaa cacattaaag aaccttgcgt caaatgccgt ggcgcggggc ggaataaggc
      961 ggtcaagacg gtggaagtca atattcccgc cggtatcgat gacgggcagc gtatccgttt
     1021 gagcggcgaa ggcgggccgg gtacgcacgg tgcgcctgcc ggcgacctgt atgtaaccgt
     1081 ccgcattcgg gcgcataaga ttttccaacg cgacggtctg gatttgcatt gcgaactgcc
     1141 gatcagtttt gccacggcgg ccctgggtgg tgagttggaa gtgccgacct tggacggcaa
     1201 agtcaagctc accgtcccca aagaaaccca aaccggcagg aggatgcgcg tgaagggtaa
     1261 gggtgtcaaa tctttacgta gcagcgcgac cggcgatttg tactgccata ttgttgtcga
     1321 aacgcctgtc aatttgaccg accgtcaaaa agagcttttg gaagaatttg agcggatttc
     1381 taccggcttg gaaaaccaaa caccgcgcaa gaaatcgttt ttagacaagc tgcgcgattt
     1441 gtttgattga ttttaaggtt cggaaacaag cagccgtatc ggggaatctc cttgatacgg
     1501 ctgtttttat ttgtttaaaa atagttttta ttttcaatgg ggtatgaggc agggtgggat
     1561 aactgttttt aactgttctt tttaaaactt gacatcatgg cgtgatgcca acaatatgtg
     1621 aacgtctgtt gtcaaaggaa gaataatgaa taaatcttta tccagttcgg tagaagaata
     1681 ccgcgagctg acgctccgag gcatgatact cggtgcattg atcactgtaa tttttactgc
     1741 gtccaatgtt tacctcggtt tgaaagtcgg gctgaccttt gcctcgtcga ttccggcggc
     1801 ggtgatttcg atggcggttt taaagttttt caaaggcagc aatattttgg aaaacaacat
     1861 ggtgcagacc caagcctcgg ctgcgggtac gctttcaacc atcatcttcg tcctgcccgg
     1921 tttgctgatg gcgggctact ggagcggttt cccgttctgg cagacgacgc ttttatgtat
     1981 tgccggcggg attttggggg tgattttcac cattccgctg cgttacgcga tggtggtgaa
     2041 aagcgatttg ccttatccgg aaggtgtggc ggctgctgaa attttgaaag tgggcggtca
     2101 tgaagaaggg gataaccgtc agggcggcag cggcatcaaa gagctggcgg ccggcggtgc
     2161 gttggcggga ttgatgagct tttgcgccgg aggtctgcgt gtgattgccg atagcgcgag
     2221 ttattggttt aaaagcggta cggcaatttt ccaactgccg atgggctttt cactggcatt
     2281 gttgggcgcg ggctatttgg tcggactgac gggcggtatc gccattctgt tgggcatttc
     2341 gattgcttgg ggcattgccg tgccgtattt ttcatcgcac atcccgcagc cttccgatat
     2401 agaaatggcg gcgtttgcga tgaagctgtg gaaggaaaaa gtacgcttca tcggcgcggg
     2461 aaccataggt atcgcggcgg tttggacgct tttgatgctg ctcaagccaa tggtggaagg
     2521 catgaagatg tcgttcaaga gttttggcgg cggtgcgccc gcaacagaac gcgctgaaca
     2581 ggatttgtcg cctaaagcca tgattttctg ggtattgtct atgatgttta ttctaggcgt
     2641 gtcgttttac cactttatcg gcgattcgca cattacgggc ggcatggctt ggcttttggt
     2701 ggtcgtttgc acgcttttgg cttccgtcat cggctttttg gtcgccgccg cctgcggtta
     2761 tatggcgggt ttggtcggct cgtcttccag cccgatttcc ggcgtgggca tcgtttccat
     2821 cgtcgtgatt tcgctggttt tgctgctggt gggcgaatcc ggcggtttga tggcggatga
     2881 ggcaaaccgc aaatttttgc tggcactgac tttattttgc ggctcggcag taatctgcgt
     2941 ggcttcgatc tccaatgaca acctgcaaga cctgaaaacc ggctacctgc tcaaagccac
     3001 gccttggcgg cagcaagtcg ccctgattat cggctgtatc gtcggtgcgc tggttatttc
     3061 acccgtgttg gaactgcttt acgaagccta cggctttacc ggcgcaatgc cgcgcgaagg
     3121 tatggacgcg gcgcaggctt tggcagcccc tcaagcgact ttgatgacga ccatcgcgtc
     3181 gggcattttc gcccacaacc ttgaatgggt ctatatcttt accggcatcg cgattggcgc
     3241 ggtgttgatt gtcgccgatt tggtgttgaa aaaatcatca ggcggcaaac ttgccctgcc
     3301 cgtcctcgcg gtcggtatgg gcatttatct gccgccgtcc gtcaatatgc ccatcgtggc
     3361 aggcgcggtg ttggcggcgg tgttgaaaca catcatcggt aaaaaagcgg aaaaccgtga
     3421 aggccgtctg aaaaacgccg agcgcatcgg aaccttgttc tccgccggcc tgattgtcgg
     3481 tgaaagcctg attggtgtga ttatggcgtt tattattgcc ttctccgtga ccaacggcgg
     3541 ctcggatgcg ccgcttgcgt tgaatctgca aaactgggat gccgccgcct catggttggg
     3601 cttggcgttc ttcgttaccg ggatgttttt ctttgcacag cgcgtactga aggcgggtag
     3661 gtagcctgtc ggaaaaaatg ccgtctgaaa cgttcagacg gcatttttta tcgggaaagc
     3721 gtaaggcgga gcttttcggc ttgcgcccac gctttaccgg caagatcttt gggcgacagc
     3781 agcggcgcgg tttgaagcgg ccagcctatg ccgattgccg ggtcgttcca tattaaaacc
     3841 tgttcggctt caggcttgta atagtccgtg catttgtagg taagttcggt cgtgtcgctc
     3901 aaaacgtaaa acccgtgcgc gaagccttcg ggtatccaaa gctggcattg gttttgcgcg
     3961 gacagggttg cgcccgccca tttgccgaaa gtgggggagt ctttacgcat atcgacggcc
     4021 acgtcgaata cttcgccgac aactacgcgt acgagtttgc cttgtgtgtt ttcggtttgg
     4081 tagtgcaggc cgcgcaatac gcctttgccg gatttggagt ggttttcctg cacgaaggtg
     4141 cgttcgcaga cttgggtttt aaaccacccg tcgcggaagg
//
LOCUS       DAWHRS010000154         4138 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWHRS010000154 DAWHRS010000000
VERSION     DAWHRS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.154"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            324..1118
                     /locus_tag="WIB82_001828"
     CDS             324..1118
                     /locus_tag="WIB82_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ3843084.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFAQAWK"
     gene            1330..3933
                     /gene="pepN"
                     /locus_tag="WIB82_001829"
     CDS             1330..3933
                     /gene="pepN"
                     /locus_tag="WIB82_001829"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ3843085.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSTKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPARYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRVAMADANGINLDQFALWYSQAGTPVLDAQGRL
                     KDGAFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQTFPLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKVLLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGLSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQRIRAQEGLSK
                     DVGEIVGKILD"
BASE COUNT         1009 a         1110 c         1130 g          889 t
ORIGIN      
        1 ttaaaaaata cttggcaagg tgtgaaaaaa ggcgtacatt ccgctacaca gaattacaga
       61 cgcagcggag caatgccgtc tgaaaggatt ttccggtcaa tcttgcgatt ggtcggggtt
      121 tcatcggaaa cggtgaaaca aaagtttgcc ggcgtagagt tgagctacgc gggcaaagcc
      181 gcaggcgaaa gcctgtattg tttgtgaagc gtaaatctct gatttgaggt attggggcaa
      241 tcctgtgggg ggttgcctct ttttttatcc gccttttaat gacacaatag gcgcaagtct
      301 gtttttgaat gcaaggtact gccatgaata cgattttggc tttcgatatt gaaaccgtac
      361 ccgatgtgca gggtatccga acattgtacg agctgccgtc ctcgctgccc gacgatgaag
      421 tggtgctgtt tgcccagcaa aaacgccgtg ctcagacggg tggggatttt atgcagcatc
      481 atcttcatca gattgtggcg atttcgtgct gcatgcgttg ggggcaggat aaagtccatg
      541 tcggcaccat cggcgagatg gacgatggcg aagaagtcgt gattgccaaa tttttcgagt
      601 tgattgaaaa acatacgccg caactggtca gttggaacgg cggcggtttc gacctgccgg
      661 ttctgcatta ccgttcgctg ctacacggca tcaacgccgc gcgctattgg gatatgggcg
      721 acggcgattt cggcgacagc cgcgatttca agtggaacaa ctacatcagc cgctaccacc
      781 aacgtcattg cgatttgatg gatttgctcg cgctttacca gccgagggca aacgtgccgc
      841 tggacgatat ggcgaaactg tgcggttttc cgggcaagct gggtatggac ggcagcaagg
      901 tgtgggatgc gttccacgcg ggcaggctga aggaaatccg caattattgc gaaaccgatg
      961 ccgtgaatac gtatttgatg tatctgcgtt tctgtctggt cagcggcaga ttcgacgcgg
     1021 acgaatacga aatggaaatc aagcggatca gaaactatct ttccgcccaa acagaagata
     1081 aaccgcattg gtcagaattt gcccaagcgt ggaaatagaa aacctgctgt ccgaaggcgg
     1141 tacgcttgtg ccgccgtttc ggtttcagac ggcatatggt ggattaaatt caaaccggta
     1201 cagcccgtac tatctgtact gtctgtggct tcgtcgcctt gtcctgattt ttgttaatcc
     1261 gctttaaaag ctgtatctgt gctgtctgaa gccttttcca gtcaaaccaa acaatattga
     1321 aagaaaaaaa tgagcaaaac cgtccgttat ctgaaagatt accaaacgcc tgcctaccgc
     1381 atccttgaaa ccgacctgca ttttgatatt gccgaaccgc aaactgtcgt gaaatcccgt
     1441 ttgacggtcg agccgcagag ggtgggggag ccgctggtgt tggacggttc gacgaaactc
     1501 ttgtccgtca aaatcaacgg cgtggcggcg gattatgtgt tggaaggcga gactctgacg
     1561 attgcagacg tgccgtccga acgcttcacc gtcgaagtgg aaaccgagat cctgccggcg
     1621 gaaaacaaat cgctgatggg gctgtatgct tccggtggca atctgtttac ccaatgcgag
     1681 ccggagggct tccgcaaaat cacgttctac atcgaccgtc cggatgtgat gtccaaattc
     1741 acgaccacca tcgttgcgga caaaaaacgc tatcccgttt tgctctccaa cggcaacaaa
     1801 atcgacggcg gcgagtgttc agacggccgc cattgggtga aatgggaaga cccgtttgcc
     1861 aaaccgagct atctgtttgc tttggtcgcg ggcgatttgg cggtcacgga agactgtttc
     1921 accacgatgg gcggcagaaa cgtcaaaatc gagttttaca ctgccgaggc ggacaagcct
     1981 aaggtcggct ttgccgtgga atccttgaaa aacgcgatga agtgggacga aacgcgcttc
     2041 ggtttggaat acgacttgga tattttcatg gtcgtcgccg tgggcgattt caatatgggt
     2101 gcgatggaaa acaaggggct gaacattttt aataccaagt tcgtccttgc cgacagccgt
     2161 accgccaccg ataccgattt tgaaggcatc gaatccgtgg tcggacacga gtatttccac
     2221 aactggacag gcaaccgcgt tacctgccgc gattggttcc agctttcgct gaaagaaggg
     2281 ttgaccgtgt tccgcgacca agaattttcc ggcgaccgcg ccagccgcgc cgtgcgccgt
     2341 atcgaaaaca tccgcctgct gcgccagcac cagttccccg aagacgcagg tccgaccgca
     2401 catccggtgc gccccgcccg atatgaggag atgaacaatt tttacaccat gaccgtttat
     2461 gaaaaaggcg cggaagtggt gcggatgtat cacaccttgc tcggcgaaga gggcttccaa
     2521 aaaggtatga agctctattt ccaacgccac gacggacagg ctgttacctg cgacgatttc
     2581 cgcgtggcga tggcggacgc gaacggcatc aatctcgacc agttcgcctt gtggtacagc
     2641 caggcgggta cgccggtttt agatgctcaa ggccgtctga aagatggagc gtttgaattg
     2701 accattaaac aaaccgtgcc gcccacgccc gatatggcgg acaaacagcc gatgatgatt
     2761 cccgtcaaaa tcgggctgct gaaccgcaac ggtgaagcgg cggcattcga ttatcagggc
     2821 aaacgcgcga ccgaagccgt attgctgctg accgaagccg aacagacctt cccgctcgaa
     2881 ggtgtaaccg aagccgtcgt tccctcgctg ctgcgcgggt tcagtgcgcc ggtgcatctg
     2941 aactatccgt acagcgacga cgacctgctg ctcctgcttg cccacgacag cgacgctttc
     3001 acgcgctggg aagccgccca aacgctctac cgccgcgccg tcgccgccaa ccttgccgcg
     3061 ctttcagacg gcatcgagtt gccgaaacac gaaaaactgc ttgccgccgt cgaaaaagtc
     3121 atttcagacg acctcttgga caacgccttc aaagtcctgc ttttgggtgt gccgtctgaa
     3181 gccgaactgt gggacggcgc agaaaacatc gacccgctgc gctaccatca ggcgcgcgaa
     3241 gccttgttgg atacgcttgc cgtccgcttc ctgccgaaat ggcacgaatt gaaccgtcag
     3301 gcggcgaagc aggaaaacca aagctacgag tacagccccg aagctgccgg ctggcgcacg
     3361 ctgcgcaacg tctgccgcgc cttcgtcctg cgcgccgacc ccgcgcacat cgaaaccgtt
     3421 gccgaaaaat acggcgaaat ggcgcaaaac atgacccacg aatggggcat cctgtccgcc
     3481 gtcaacggca acgaaagcga tacgcgcaac cgcctgctgg ctcagtttgc cgacaagttt
     3541 tcagacgacg cgctggtgat ggacaaatat ttcgccctcg tcggcttaag ccgccgcagc
     3601 gacaccctgc aacaggtcca aaccgccttg cagcatccga aattcagcct cgaaaacccc
     3661 aacaaagccc gttcgctcat cggcagcttc agccgcaacg tcccgcattt ccacgcagaa
     3721 gatggcagcg gctaccgctt catcgccgac aaagtcatcg aaatcgaccg ctttaacccg
     3781 caggtcgccg cccgtttggt gcaagcgttc aacctctgca acaagctcga gccgcaccgc
     3841 aaaaacttgg tgaaacagga attgcagcgc attcgggcgc aggaaggatt gtcgaaagac
     3901 gtgggcgaaa tcgtcggcaa aattttggat tgaggccgtc aaacagaaaa acaatgccgt
     3961 ctgatatagt tacacacacg ttttatcatc acttccccat cgttttatta cgcaatggca
     4021 aaacggccgc aaagtcatat taattatagt ggattaacaa aaatcaggac aaggcgacga
     4081 agccgcagac agtacagata gtacggtaag gcgaggcaac gctgtactgg tttttgtg
//
LOCUS       DAWHRS010000155         4117 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWHRS010000155 DAWHRS010000000
VERSION     DAWHRS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.155"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            41..673
                     /locus_tag="WIB82_001830"
     CDS             41..673
                     /locus_tag="WIB82_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ3843086.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            745..2115
                     /gene="purB"
                     /locus_tag="WIB82_001831"
     CDS             745..2115
                     /gene="purB"
                     /locus_tag="WIB82_001831"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ3843087.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSGVPEVAAASEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTGMAHEL
                     AAVPMMSRTHGQPATPSTLGKEIANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2195..4063
                     /locus_tag="WIB82_001832"
     CDS             2195..4063
                     /locus_tag="WIB82_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ3843088.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
BASE COUNT          941 a         1096 c         1067 g         1013 t
ORIGIN      
        1 tttgacggtg tacaatcgct gtttttgaac ggaggatgga atggagaata caaaccgtgt
       61 gccggagcag gcacgttatg atgccgaacg caggcaggca gacgaagcat tggcgggcgt
      121 gtttccggca gtcagtatct tcggcagcgc gcgcacgccg cagaatcatg cggattatgc
      181 gttcgcctgc cgtctggcgc ggcggctgtc ggattcgggc attgccgtca tttcgggcgg
      241 cgggccgggg attatggagg cggcaaacaa gggcgcgttt gcagggaagt cggtttcggt
      301 ggggctgaac atcgttttgc cgcacgagca gaaaccgaat ccgtatcagg acatcgcctt
      361 gcggttttcc cgttttgccg aacgcaaggc ggtgtttttc cgctattccc aagcatatgt
      421 cgtgatgccg ggcggcttcg ggacgctgga cgaattgttt gaaatcctga ccttggtgca
      481 gacgggcaaa gtgccgccgc gtccgattgt tttggtcgga aaggcgtttt ggtcgggctt
      541 ggcggagtgg ataaacgcgc agcttttggc gcgcggtctg atttccgaag gggcggtctc
      601 tttgtttgcc atatcggacg atgaagacga aatcgttgcg tatctgtcgg aacacgggct
      661 tcagacggca tagcgtcctg agagtgatgt ataattgcaa acaatttaac aatttttgat
      721 gtctttcccg aacaggatgc cgaaatgatc aaccccatcg cctcgctttc ccctttagac
      781 ggccgttatg cccaatccgt tgaagcattg cgcccgattt tttccgaata cggcctgatg
      841 aaagcgcgcg tcaaagtcga attaaactgg ctcaaagccc tcgccgccga gccgaagatt
      901 gccgaagtgc cgcccttcag tgccgaaacg cttgccgaaa tcgacacggt gattgaaaac
      961 ttttcattgg aagacgcggc cgccgtcaaa gccatcgaag ccacgaccaa tcatgatgtc
     1021 aaagccattg aatattggct taaagaacgc ttctccggcg tgcctgaagt ggcagctgcc
     1081 agcgagttca tccactttgc ctgcaccagc gaagacatca acaacctgtc ccacgcttta
     1141 atgctgcaag aagcgcgtga ggctgttttg ctgccgaaac tggccgaaat catcgaaaaa
     1201 ctgaccggca tggcgcatga gttggctgcc gtcccgatga tgagccgcac ccacggccag
     1261 cctgctacac caagcacact gggtaaagaa atcgccaatg tcgtgtaccg cctgcaacgc
     1321 cagtttaaaa acctgcaagc gcaagagttc ctcggcaaaa tcaatggcgc agtcggcaac
     1381 tacaatgccc atatggtcgc ttatcccgat gtggattggg aaacccattg ccgcaacttc
     1441 gttgaaatca gcctcggcct gaccttcaac ccctacacca ttcaaatcga gccgcacgac
     1501 tatatggcgg aattcttcca agccatcagc cgtgtcaaca cgattctgat cgacttcaac
     1561 cgcgatgttt ggggctatat ttctttgggt tacttcaaac aaaaagtcaa agcaggcgaa
     1621 gtcggttctt ccaccatgcc gcacaaagtc aacccgatcg actttgaaaa ctccgaaggc
     1681 aacctcggta tggcaaacgc cgtattgggc ttcttatccg aaaaactgcc gatttcccgt
     1741 tggcagcgcg acctgaccga cagcaccgta ttgcgcaata tgggcgtagg cgtgggctat
     1801 gccgtgttgg gtttcgccgc ccatctgcgc ggtctgaaca agctcgaacc caaccctgcc
     1861 gcgcttgccg ccgatttgga cgctacttgg gagctgctcg ccgagccgat tcaaaccgta
     1921 atgcgtcgtt acggcgtagc caatccttat gagaagctga aagacctgac gcgcggcaaa
     1981 gacggcatta cgcctgaagt gttgaaactc tttatcgagt cgttggaaat tccggcagaa
     2041 gccaaagcca aattgcttga gctgaccccc gcactgtatg tgggtaaggc tgaagcgttg
     2101 gcgaaacgga tttgagcgtt tactgaaacc gatgccgtct gaacgcgcgt tcagacggca
     2161 tttttaagat aacgggacat acggggcgat atttatgcaa gctgtccgat acaggcctga
     2221 aattgacgga ttgcgggccg ttgccgtgct atccgtcatt attttccacc tgaataaccg
     2281 ctggctgccc ggagggtttt tgggggtgga cattttcttt gtcatctcgg gattcctcat
     2341 taccggcatc attctttctg aaatacagaa cggttctttt tctttccggg atttttatac
     2401 ccgcaggatt aagcggattt accctgcctt tattgcggcc gtgtcgctgg cttcggtgat
     2461 tgcctctcaa atcttccttt acgaagattt caaccaaatg cggaaaaccg tggagctttc
     2521 tgcggttttc ttgtccaata tttatctggg gtttcagcag gggtatttcg atttgagtgc
     2581 cgacgagaac cccgtactgc atatctggtc tttggcagta gaggaacagt attacctcct
     2641 gtatcctctt ttgctgatat tttgctgcaa aaaaacaaaa tcgctacggg tgctgcgtaa
     2701 catcagcatc atcctatttc tgattttgac tgccacatcg tttttgccaa gcgggtttta
     2761 taccgatatt ctcaaccaac ccaatactta ttacctttcg acactgaggt ttcccgagct
     2821 gttggcaggt tcgctgctgg cggtttacgg gcaaacgcaa aacggcagac ggcaaacagc
     2881 aaatggaaaa cggcagttgc tttcatcact ctgcttcggc gcattgcttg cctgcctgtt
     2941 cgtgattgac aaacacaatc cgtttatccc gggaatgacc ctgctccttc cctgcctgct
     3001 gacggcactg cttatccgga gtatgcaata cgggacactt ccgacccgca tcctgtcggc
     3061 aagccccatc gtatttgtcg gcaaaatctc ttattcccta tacctgtacc attggatttt
     3121 tattgctttc gcccattaca ttacaggcga caaacagctc ggactgcctg ccgtatcggc
     3181 ggttgccgcg ttgacggccg gattttccct gttgagttat tatttgattg aacagccgct
     3241 tagaaaacgg aagatgacct tcaaaaaggc atttttctgc ctctatctcg ccccgtccct
     3301 gatacttgtc ggttacaacc tgtacgcaag ggggatattg aaacaggaac acctccgccc
     3361 gttgcccggc gcgccccttg ctgcggaaaa tcattttccg gaaaccgtcc tgaccctcgg
     3421 cgactcgcac gccggacacc tgcgggggtt tctggattat gtcggcagcc gggaagggtg
     3481 gaaagccaaa atcctgtccc tcgattcgga gtgtttggtt tgggtagatg agaagctggc
     3541 agacaacccg ttatgtcgaa aataccggga tgaagttgaa aaagccgaag ccgttttcat
     3601 tgcccaattc tatgatttga ggatgggcgg ccagcccgtg ccgagatttg aagcgcaatc
     3661 cttcctaata cccgggttcc aagcccgatt cagggaaacc gtcaaaagga tagccgccgt
     3721 caaacccgtc tatgtttttg caaacaacac atcaatcagc cgttcgcccc tgagggagga
     3781 aaaattgaaa agatttgccg caaaccaata tctccgcccc attcaggcta tgggcgacat
     3841 cggcaagagc aatcaggcgg tctttgattt ggttaaagat atccccaatg tgcattgggt
     3901 ggacgcacag aaatacctgc ctaaaaacac ggtcgaaata cacggccgct atctttacgg
     3961 cgaccaagac cacctgacct atttcggttc ttattatatg gggcgggaat ttcacaaaca
     4021 cgaacgcctg cttaaatctt ctcgcgacgg cgcattgcag tagcctgcct tgccgtccga
     4081 tatcgtttgt gccgccgttt gcctttcggg gcggcgg
//
LOCUS       DAWHRS010000156         4102 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWHRS010000156 DAWHRS010000000
VERSION     DAWHRS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.156"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1107)
                     /locus_tag="WIB82_001833"
     CDS             complement(<1..1107)
                     /locus_tag="WIB82_001833"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HEZ3843089.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVHEQGQKLQTALLDLCRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPS"
     gene            1511..2011
                     /locus_tag="WIB82_001834"
     CDS             1511..2011
                     /locus_tag="WIB82_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843090.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            2096..2650
                     /locus_tag="WIB82_001835"
     CDS             2096..2650
                     /locus_tag="WIB82_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843091.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            2664..2864
                     /locus_tag="WIB82_001836"
     CDS             2664..2864
                     /locus_tag="WIB82_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843092.1"
                     /translation="MPSEELSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(3139..>4102)
                     /locus_tag="WIB82_001837"
     CDS             complement(3139..>4102)
                     /locus_tag="WIB82_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ3843093.1"
                     /translation="SGKVDFERLEVLILDEADRMLDMGFIDDIETIVEATPSDRQTLL
                     FSATWDGAVGKLARKLTKDPEIIEVERVDDQGKIEEQLLYCDDMRHKNRLLDHILRDA
                     NIDQCVIFTSTKAMTEVIADELYEKGFAANCLHGDMPQGWRNRTLMDLRKGRCKILVA
                     TDVAARGIDVPTITHVINYDLPKQAEDYVHRIGRTGRAGRTGIAITFAEVNEYVKVHK
                     IEKYINRKLPELTIEGMEPTRKRKSAGGKPKGKGGWGDRKSGGWRGDHKPSKEGFGGK
                     TRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDSFAGKGERRYKDR"
BASE COUNT          942 a         1160 c         1057 g          943 t
ORIGIN      
        1 cgaaggcgcg aaacgcaata cgtccgcacc cgcaaccaaa atcatcacgc cgtgtttcaa
       61 ggcggcggcg gtgatttcgg atgcgcgtcc gcgataggct tcgtccaaca cgcagccgag
      121 tagcagcccc atcccgcgaa cttgtgagaa caagcccgtt ttcctgcaca aatccagcaa
      181 tgccgtctga agtttctgcc cctgttcatg gacatggttt aaagtttcgg gcgtattgat
      241 gatgtcgaat gcgcggctgc cgaccgcaca cgccatcggg ttgccgccga aagtcgagcc
      301 gtgcgtgccc ggttggaagg cggcggcaat cttttctgtc gccagcatcg cgccgatcgg
      361 aaagccgcag cccaaggctt ttgccgaact caaaatatcg ggaacaatgc cgtaatgttc
      421 ataggcaaac agcctgcccg tatgccccat cccggtttga acttcgtcca aaatcaacaa
      481 cgcattgtgc cggtcgcaca gacggcgcgc ggtttgcaaa tattccgcag tggcgggcag
      541 gatgccgctt tcgccctgta tcggctcgat gatgaccgcg caggtctgtt cgccgacggc
      601 agcttccagc gcggcaatat cgttgaacgg aacgtgcgta atgccttgcg gcaggggtgc
      661 ataatccttg ctgtatttcg gctgaccgcc gacggacacg gtaaacagcg tgcgtccgtg
      721 aaaactgttg atacaggcga cgatttcgct ttttcctccg ccgaaacggt cgcgggcgta
      781 tttcctcgcc agcttcaacg ccgcctcatt cgcttccgag cccgagttgc agaaaaaaac
      841 cttgtcggca aaactgtttg caaccaattt ttgcgccaat tcctgcgctg gacgcgtcgt
      901 atagatattg gaaatgtgcc acagcttgtg catctgcgcg tttaaagcat cgacaagggc
      961 agggtggcag tgtcccagcg cattgacggc gataccgcct gaaaaatcaa tatattcacg
     1021 ccctttcgta tcccaaacgc ggctgcctga agcgcgttcg ggaatcatcg gggcaaaggc
     1081 gaaattgggg gtcagatagt tttgcataat tgctcctgat tgcggttttg ctcgggatta
     1141 tgcaccgttt ttcaaagcag ataaagggaa attgccgaca atatcgggca gcggtaaaaa
     1201 taaaccgaaa tattttcaat cggatagatg aaaaatcaaa aaaatttcga taaaatgctt
     1261 gaggcgcggc agcgttttga tttagaatct ccccaatatt tttatcttca gacggcattt
     1321 catcatgcaa ggcaaaaaat ttacaaccgc ttattgctat tggtaccaca cagacgtgcg
     1381 gtactttttt gcgcttgctt gaaatccatc ccatacgcaa aacaggccgc tcgaaaacgg
     1441 ggcggctttt ttgttacctt cacacaggtc gcccgattcg gcaacacacg acagaatcag
     1501 gaaaatgacc atgaatgcaa tgccccaaac cgccgcacaa aacgaaaccc tgcacacttg
     1561 tagcattatt gtcgatgatg cacaggattt ttcccccatg cttctcgatg cagggcggga
     1621 cgacacactt atcagcgaat cgatgattcc gcaaatccgc accgttgccg ccctgatagc
     1681 cgccgaacgg cacgacttca gccgcagcag ccccgccgag tttaccgatg ccgccgattt
     1741 ttttgccgca cggattctcg tattgggcgt gcgacgcttc catctggacg tatcgctgct
     1801 tcagattttg aaaaccgcca acagccgcgc ccgccgcttt gccgaaaaac accgcctgtt
     1861 tttcacaccc gcacaagccg aattgagcct gaacaaacac aaaaaccgcc gcctgctgac
     1921 cgtagaaacc gaacacgaag tcgaaaacaa aggcaaccca gtcgccaaca gcctcgcatt
     1981 tgtccgcaaa ttgcacaccc tgcccctgta aacatcacga tttgccggca gaatgcttgc
     2041 caaaattccc gacggcgata caataaaacc gattttccac ccaaacagga cacacatgaa
     2101 aaaaatcatc gcctccgcgc ttatcgcaac attcgcactc gccgcctgcc aagacgacac
     2161 gcaggcgcgg ctcgaacagc agcagaaaca gattgaagcc ctgcaacagc agctcgcaca
     2221 gcaggcagac gatacggttt accaactgac tcccgaagca gtcaaagaca ccattcctgc
     2281 cgaagcacag gcaaacggca acaacgggca acccgttacc ggtaaagacg ggcagcagta
     2341 tatttacgac caatcgacag gaagctggct gctgcaaagc ctggtcggcg cggcggcagg
     2401 cgcgtttatc ggcaacgcgc tggcaaacaa attcacacgg gcaggcaacc aagacagtcc
     2461 cgtcgcccgg cgcgcgcgtg cagcctacca tcagtccgca cgccccaatg cgcgcaccag
     2521 cagggatttg aacacgcgca gcctccgtgc aaaacaacag gcggcgcagg cgcagcgtta
     2581 ccgcccgaca acgcgcccgc ccgccaatta ccgccgcccc gctatgcgcg gtttcggcag
     2641 gaggcggtaa acccggcgcg ccaatgccgt ctgaagagct ttcagacggc atttttgcat
     2701 ttgttaggga cattgttatg ttgccgtttg attttcagac ggcattttgt ttccaagcgt
     2761 ttgatgtcgg gatggcaatt ctgatttacg tgcgttggca gggtggcatt gtcatgccgc
     2821 cgttgggttt tcagacggca tcatatcgaa cagtcaagcc gtagggcgag cattcttgcc
     2881 caccgttgca agggcgggat tgggtgggca ggaatgccca ccctgcatct gaaacccttc
     2941 cgtgtcattc ccgtgcagga aggaatctag atatttgaat ttacggaaat tttcaatcgt
     3001 tgccaaagcg gaaaagggtt tttattacag cctaatcctt tgggtatcaa agccgggcgg
     3061 tgctgaaaca ttgcaaaaat gccgtctgaa actcgaaatc ggtttcagac ggcattggtt
     3121 tatgcggcag gttggggctt agcggtcttt gtaacggcgt tcgcctttgc ctgcaaaaga
     3181 atcgccggct ttgcgtttgc ctttgaagcc ttcgccggtt tttttgaaac cgtctctctt
     3241 aaagccttct ttcttgaaac cttcgccgcg cgttttgccg ccgaagcctt ctttgctcgg
     3301 tttatgatcg ccgcgccaac cgccggattt acgatcgccc cagccgcctt tgcctttcgg
     3361 cttgccgcct gcggatttgc gtttgcgggt cggttccatg ccttcgatgg tcagttcggg
     3421 cagttttcgg ttaatgtatt tttcgatttt gtggactttg acgtattcgt tcacttcggc
     3481 aaacgtaatc gcaatacccg tgcggcctgc gcggccggtg cgcccgatgc ggtggacgta
     3541 gtcttccgcc tgtttcggca ggtcgtagtt gataacgtgg gtaatggtcg gtacgtcgat
     3601 accgcgtgcg gcaacatcgg tggcaaccaa aattttgcag cggcctttac gcaaatccat
     3661 cagcgtgcgg ttgcgccagc cttgcggcat atcgccgtgc aggcagtttg cggcgaaacc
     3721 tttttcgtac agttcatccg caatgacttc ggtcatggct ttggtggacg tgaaaatcac
     3781 gcattgatcg atattggcat cgcgcaagat atgatcgagc aggcggtttt tgtggcgcat
     3841 atcgtcgcag tacagcagtt gttcttcgat tttgccttga tcgtccacgc gttcgacttc
     3901 gatgatttca gggtctttgg tcagtttgcg cgccagtttg ccgaccgcgc cgtcccaagt
     3961 ggcggagaac aacaaagtct gacggtcgct cggcgttgct tccacgatgg tttcgatgtc
     4021 gtcgataaag cccatatcca acatacggtc ggcttcgtcc aaaatcagca cttccaaacg
     4081 ttcaaaatca actttgccgc tt
//
LOCUS       DAWHRS010000157         4098 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWHRS010000157 DAWHRS010000000
VERSION     DAWHRS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.157"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(403..1215)
                     /locus_tag="WIB82_001838"
     CDS             complement(403..1215)
                     /locus_tag="WIB82_001838"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ3843094.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1405..1866
                     /gene="tsaE"
                     /locus_tag="WIB82_001839"
     CDS             1405..1866
                     /gene="tsaE"
                     /locus_tag="WIB82_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ3843095.1"
                     /translation="MPDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLEPFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            1863..3113
                     /locus_tag="WIB82_001840"
     CDS             1863..3113
                     /locus_tag="WIB82_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ3843096.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     PNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMLDPGHGGEDPGAISPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKQG"
     gene            3193..4011
                     /locus_tag="WIB82_001841"
     CDS             3193..4011
                     /locus_tag="WIB82_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3843097.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
BASE COUNT          995 a         1205 c          999 g          899 t
ORIGIN      
        1 gggaatgacg gtttagaagt tacccgaaac ctcaaaaaaa ccgaaaccga acggtctgga
       61 ttcccgcctg cgcgggaatg acgggtttcg agattacagt gtatcgagaa ggatgggaaa
      121 cggtgggaat tgtgtaaaaa atgccgtctg aagcctttca gacggcattt gcggcattcg
      181 gacatttaaa aatcccatca ttcccgcgca ggagggaatc cgaatacatc tgcacagaaa
      241 cctgcatccc gtcattcccg cgaaagcggg aatctagaac gtaaaatcta aagaaaccgt
      301 tttatccgat aagtttccgc accgacaggt ctagattccc actttcgtgg gaatgacggg
      361 attttaggtt tctgtaatct cgggcagctt tccgtcatcg ttttaaccca aagacaccat
      421 ttcaatctgc tccatcgtcc tgcccagaaa acgctcgccg atggttctga atttcaaagg
      481 aatatcgctg acataaaaac ggtagtcggg attgttgttg tcggtattga gcaatccttc
      541 ctgcgcaagg acgcgtgcgg tttcttcggc agtcgtaatc gcggaatcga ccagcgcgac
      601 attgtgcgcc tccctgccga ttaagggttt gagcaaggga aagtgcgtgc agcccaacac
      661 cagcgtatcg atgccgtctg caagcaatgg tttgaggtat tcgcatacgg tcaggcgggt
      721 aacttcgtgt tccaaccaac cttcctccac caaagggacg agcagcggcg cggcctgcgt
      781 gcggacaagc gtgtcggggt tgtccctatg gatggcgcgc gcataagcat tgctgttaac
      841 tgtggtattg gtggcgataa tgccgatttt gttgttgcgc gtcgttgcca acgcggcttt
      901 cgcgccggcg gaaatcacgt ccaaaaccgg catattgccc gtcttttgac ggattttctg
      961 ccccgccacc gccgcaatcg tattacacgc gataaccatc gccttgacat cgtgttccaa
     1021 taaaaaatcg acaatctgca tcgagaaatt ttcgatggtc gccttagatt tcgtcccgta
     1081 aggcacgcgc gccgtatcgc cgaaataaat gatgttctcc atcggcagcc gctccatcag
     1141 tgcgcgcaca ttggtcaaac cgccgattcc cgagtcaaaa acgccgatgg gtcgctgcct
     1201 gccgatattt tccatccttt ttccaatccg tcctaaacat acaatccgcg tattgtacac
     1261 gcgccgcttt ttcttgacag ccgttgccgt ctgaaagcag gatgcggatt tggctgttat
     1321 aatgtacggc aatgctgccg ccgggcgcac ggtttgccga ccgcctgccc ctccgatccg
     1381 ccacataaaa gttgtaaagc ccctatgcct gattttcccc ccgtttcccg attccttgcc
     1441 gacgaagccg ccacactcga tttgggcgcg gcgtggtctt cccgtttaaa cgcaccgctg
     1501 gtcatttatc tcgaagggga tttgggtgcg ggcaaaacca cgctgacacg cggcatcctg
     1561 cgcggattgg gtcatcaggg cgcagtcaaa agcccgacct acgccatcgt cgaatcttat
     1621 ccgctggaac cctttaccct gcaccatttc gacctctacc gcttctcgtt ccccgaagaa
     1681 tgggaagacg cggggcttga cgaactgttt gccgcaaaca gcgtctgcct gatcgaatgg
     1741 ccgcaacagg gaggcgaatt taccccgccc gccgacatca ccgcaacatt gacacacgac
     1801 ggcggcggca gaaaatgcct gctgaccgcc cataccgaac gaggacgcga aagcctgccg
     1861 ctatgaccaa actgacacga agacaaatca tccgccgcac cgccggcaca ctgttcgccc
     1921 taagccccat cgcatccgcc gttgccaaaa cggtacgcgc cccgcagttt accgccgcac
     1981 ggatatggcc gtcgcacacc tacacccgcc taacgctgga aagcaccgcc gcgctcaaat
     2041 accagcactt cacgctcgac aacccgggca ggctggtcgt cgatatacaa aacgcgaaca
     2101 tcaataccgt attgcacgga ctgtctcaga aggttatggc ggacgacccc tttatccgca
     2161 gcatacgcgc gggtcagaac acaccgacca ccgtccgcct cgtcatcgat ttgaaacagc
     2221 ccacccacgc acaagtcttc gcgcttccgc ccgtcggcgg ctttaaaaac cgcctcgtcg
     2281 tcgatctcta tccgcaaggg atggatgccg acgatccgat gatggcgctg cttaacggca
     2341 gcccgaacaa aacccggcgc ggctctccgg aagccgacct cgctcaaaac actcccgcac
     2401 aaaccgaacg cggcaaaaac gggcgcagac ccgtcatcat gctcgatcca ggacacggcg
     2461 gtgaagaccc cggcgccatc agcccgggcg gtttgcagga aaaacacgtc gtcctctcca
     2521 tcgcccgcga aaccaaaaaa caattggaag ccttgggtta caacgtcttt atgacgcgca
     2581 acgaagacgt gttcatccca ttgggcgtgc gtgtcgccaa agggcgagca cggcgggcgg
     2641 acgtattcgt ctccatccac gccgatgcct tcaccagccc ctccgcgcgc ggcacggggg
     2701 tttatatgtt aaacaccaaa ggcgcaacca gctctgccgc caaattcttg gaacaaaccc
     2761 aaaataatgc cgacgcggtc ggcggcgtac cgaccagcgg caaccgcaat gtcgataccg
     2821 ccctgctcga tatgacccaa accgccacgc tgcgcgacag ccgcaaactc ggcaaactgg
     2881 tgcttgaaga attgggcagg ctcaaccact tgcacaaagg cagggtggac gaagccaatt
     2941 tcgccgtttt gcgcgcaccc gatatgccgt ctatcctggt cgaaaccgcc ttcctgtcca
     3001 accctgccga agagaagctg ctgggcagcg aatccttccg ccgacagtgc gcccaatcca
     3061 ttgcctcggg tgtccaacgc tacatcaaca cttccgtatt gaagcagggt tgattgaaaa
     3121 ggcggtgttt caatatagtt gtcggatgaa ggcaggcata aacctacttc cgccccaaac
     3181 cgcgttttta tgatgaaagg tatggataaa ttgagatacc aacaggattt tttaaatatc
     3241 cgccccattt ttacagccgg cgaacaagaa tacctgaccg aattgtccga tcggcttcct
     3301 ttatctgttt taacggactc cgtgcgtaat attgaagaaa tcggtatcga ttttgtgtac
     3361 agttctgcca aattggaagg aaacacttat aatcaatacg atacgcaggc attgcttaaa
     3421 ctcgggcaaa ccgcaggcgg gaagttatat tcggatgccg taatgttaat caacctgagg
     3481 gaaagttacc ggcacctgct gtccggtttg gacagccccg atccgtttga ctggttggat
     3541 ttcttaaaaa ccacccacag cctgatttcg gaaaacttac tggaaaaagg ctccggcggc
     3601 gtagtccgcc gcgacagcgt tactataagc gggacagact acacgccttt atccaacccg
     3661 caaagtttgg atacggaatt aaaatggttg cttcaagaag cccccaaaat agaaaatcct
     3721 ttcgaccgcg ccgtttatct ccataataat ttggcgtatc ttcaatattt taaagattgc
     3781 aataaacgta ctgccagaaa ctgcatgacc ctatcgctga tgcgctccgg tttttttcct
     3841 tgcgtattcg cccccgacag ttatcccgcc tatgccgagg ctgtcgtagc ctattacgaa
     3901 accggcgatt acggtttatt caagaaatat tttatttcgg catacgaaaa taccgtaaat
     3961 aagtacggcc cacagcctga tatggatatt tcccgcaact tctcccttta attccaaggg
     4021 gttgttatcc gtatcttccg cacgcggcaa atgcggcggc acagtggaca cacccctccc
     4081 ttaccttcgg ttatagtg
//
LOCUS       DAWHRS010000158         4047 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWHRS010000158 DAWHRS010000000
VERSION     DAWHRS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.158"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(99..1460)
                     /gene="accC"
                     /locus_tag="WIB82_001842"
     CDS             complement(99..1460)
                     /gene="accC"
                     /locus_tag="WIB82_001842"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ3843098.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(1574..2026)
                     /locus_tag="WIB82_001843"
     CDS             complement(1574..2026)
                     /locus_tag="WIB82_001843"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ3843099.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQQ
                     VKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(2008..2265)
                     /locus_tag="WIB82_001844"
     CDS             complement(2008..2265)
                     /locus_tag="WIB82_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ3843100.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            2382..3422
                     /gene="queA"
                     /locus_tag="WIB82_001845"
     CDS             2382..3422
                     /gene="queA"
                     /locus_tag="WIB82_001845"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ3843101.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
     gene            complement(3896..>4047)
                     /locus_tag="WIB82_001846"
     CDS             complement(3896..>4047)
                     /locus_tag="WIB82_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843102.1"
                     /translation="DVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHL
                     EKVFG"
BASE COUNT          870 a          983 c         1210 g          984 t
ORIGIN      
        1 ttaatccact atatcgaaat cggtttcaga cggcgcgggg caagggaaat gccgtctgaa
       61 cgcggacggg cggcttcaga cggcatatcg gcggcggttt acttgtcctg tttggctttg
      121 cgatcttcca gccagtgttc caaatagtgg atgctgacgc cgcctttttg gaaacccgca
      181 tcggcgaaca ggtcgcggtg aagcggcgta ttggttttga tgccggttac cgccagctcg
      241 gcgagtgcga cgcgcatttt cgccattgcc tgttcacgcg ttttgccgtg tacgcagatt
      301 ttgccgatca ggctgtcgta gtacggtggg atgcggtagc cttggtaaat gtggctgtcc
      361 acgcggatac cgaagccgcc gggcaggtgg cagctttcaa tcgggcccgg gctggggatg
      421 aagttgtacg ggtcttcggc attgatgcgg cactcaaacg cgtggccttc gacttgaata
      481 tccttttgtt tgtattgcaa aggcaggccg gcggcgatgc ggagttgctc ctgcacgatg
      541 tccacgccgg taatcagctc ggtaaccgga tgctcaacct gaacgcgcgt attcatctcg
      601 ataaagaaaa attcgccatc ttcgtacagg aattcaaacg tacctgcgcc acgatagccg
      661 atgcgtttgc aggcgtcggc acaggcgttg ccgattttgg cgcgttcttt ttcagtgatg
      721 aacggagccg gtgcttcctc gatgactttt tggtggcggc gttgcagcga acagtcgcgc
      781 tcggcaaggt agatggcgtt gccgtgttcg tcggcaatca cttggatttc gacgtggcgc
      841 ggacgttgca gatagcgttc catgtaaacc atcgggttgc cgaatgccgc gcctgcttct
      901 gccttggtca tttcgacaga ttggaggagg tcttcttttt tctcgaccac gcgcataccg
      961 cgaccgccgc cgccgccaga ggctttgata atgacgggat aaccgacttt atcggcgatt
     1021 ttgaggattt cttcgccgtc gtcgggcaat gcgccgtcag aaccggggac gcagggtacg
     1081 cctgccgcta tcatcgcgtg tttggcggag actttgtcgc ccatcaggcg gatggtgtcg
     1141 ggtttcgggc cgataaaggt aaagccggac tgctcgacct gttcggcgaa atcggcgttt
     1201 tcggcaagga aaccgtagcc cggatggaca gcgtccgcgc agcttacttc ggcggcggcg
     1261 ataatggcgg ggacgttaag gtagctttgc gcggaagcgg cagggccgat gcacacggat
     1321 tcgtcggcga gtttgacgtg caggctgtct ttgtcggcct cggaatgcac ggcgacggtg
     1381 gcaatgccca tttcgcggca ggcacggagt acgcgtaatg cgatttcgcc tcggttggcg
     1441 attaaaactt ttttcagcat gatgactgtt cctgtggttc cggcaacccg tccgtaaaaa
     1501 aaaaggtgcg ggaacgccga agggggaaag catttcagac ggcatcggaa gtttatgccg
     1561 tctgaaaaca ggattatccg atgatgaaga gcggttcgcc gaattcgacg ggcgtaccgt
     1621 tttcgaccag aatttctttg accgtgccgg atttttcggc ttcgatttcg ttcatcagct
     1681 tcatcgcttc gatgatgcac agcgtgtcgc cggctttaac ctgttggccg acttcgacaa
     1741 aaggcgcggc attcgggccg ggtgcgcggt agaacgtgcc gaccataggc gatttttgcg
     1801 cgtcggacaa atcgcgggcg gcaggtgcgg cgggcgcgga tgccgcaacg ggtgcggcgg
     1861 caggcgttac ggccggcgcg gcggcaggta cgggcgcggc gtaaacgggt gcggcggcga
     1921 tggttcgggt gatgcggact ttttcctcgc cttcggttac ttcgatttcg gcgatacccg
     1981 attcttcaac caaatcaatc agttttttta atttgcgcaa atccatttct gttcctttaa
     2041 aggctgccgg tgttccggcg ggttgttgcg ttggtttttc agaaaacagc cggcttgcgg
     2101 caacggcgca aaccgggcgg aatggtgttc ggatacggtt ttcctgtggt ggaaaacaac
     2161 cgtgagaatg tgtttatttt cccgaagttg tcggtaaatg tccagtaaaa tatcaaaaaa
     2221 cggcggtttt tgggggaaat gtgcaggaag cttgattttg cgcacaagat gccccgggtc
     2281 aagagtgttt attctaatct gttggttttt cggacaaaga tgccgtctga aaaagggcta
     2341 aagtgcgtat aatggcggct tgcccgaacg agagtgtaaa aatggatatt tcagattttg
     2401 attttaccct gcccgaaaag ctgattgccc agcatccgcc cgaagtgcgc ggcagcagcc
     2461 gccttttggt tgcgttgccc gatatgccgc tgcaagaccg ggtgttcggc gatttgccgg
     2521 attatgtcga ggcgggcgac gttttggtgt tcaacaacac caaagtcatg aaggcgcggc
     2581 tgtttgggca gaaagacagc ggcggcagga tcgaagccct gattgagcgc gtgttggaca
     2641 accataccgc actggcgcac atccgttcgt ccaagtcccc caagcccggt atggggctgg
     2701 tgtttgaagg cggcatccgt gccgtgatgg tcgggcgtga gggcgaactg ttctgcctgc
     2761 gttttgaagg cggtcaaacc gtttacgaac ttttggaaca gaacggccat ttgcctctgc
     2821 cgccttatat cgaacgtgcc gccgacgcgg acgacgacag ccgttatcaa accgtttatg
     2881 ccaaatatca gggcgcggtc gccgcgccga cggcgggcct gcattttacg gaagaacttt
     2941 tgcaccgtct gaaagacaaa ggcgcggtaa ccgcagaagt aactctgcac gtcggtgcgg
     3001 ggacattcca acccgtgcgt gtggaaaaaa tcgaagaaca caaaatgcac agcgaatggt
     3061 ttgaagtgcc gtctgaaacc gccgccgccg ttgaggcggc aaaagcccgg gggaacaaag
     3121 tctgggcggt cggcacgact tccatgcgcg ccctcgaatc tgccgcgcgt gcgacgggtc
     3181 gtctgaaagc ggggcagggc gacaccgata ttttcatcac gccgggctac cgttttaatg
     3241 ttgccgacag gctggttacc aatttccatc tgccgaaatc gacgctgctg atgcttgtca
     3301 gcgcgttttc gggtatgggt catatccgcg ccgcgtatcg ttatgcggtc gaacgcgaat
     3361 accgtttctt cagctacggc gatgcgatgg ttttggggcg gaacgaaggg gtggtacgtt
     3421 agaacagcct caagtctatt gggggaagac aagtgccgtc tgaacggatg ttcggacggc
     3481 attcttttgt tgatttgaat gaaaggaact gcatgacata tagtggatta aatttaaatc
     3541 aggacaaggc aacgaagccg tagacagtac aaatagtacg gcaaggcgag gcaacgctgt
     3601 actggttttt gttaatccac tatagattca ggcgcgagag gcgagaaact cagtcattgt
     3661 tgatgacgcg caatacgtta aaattcattc cgttttggtc gtataaacat attacatacg
     3721 ccattttttt gtattcaagg ctgcctgaaa acagtgggca agaatatcta acccaacgaa
     3781 aagctgaaaa agcaacaaaa aataatctgc accttagtcg gttggttagt ccaaccttag
     3841 gggtgcagac cattcaatct ttcaggctgc ctttgtttgt ttataagata aatttttagc
     3901 cgaacacttt ttccaagtgt gcctgataat ctgccaagta tttttccact tgtggatttt
     3961 taaccacatc gttacataag aatgtcggca ggcgggtcat acccaaaaac tcgttggctt
     4021 tgtggaagtg catatacaaa acatcaa
//
LOCUS       DAWHRS010000159         4019 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWHRS010000159 DAWHRS010000000
VERSION     DAWHRS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.159"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(88..282)
                     /locus_tag="WIB82_001847"
     CDS             complement(88..282)
                     /locus_tag="WIB82_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ3843103.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(272..2353)
                     /locus_tag="WIB82_001848"
     CDS             complement(272..2353)
                     /locus_tag="WIB82_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ3843104.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPDRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVVLWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2830..3210
                     /locus_tag="WIB82_001849"
     CDS             2830..3210
                     /locus_tag="WIB82_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ3843105.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3292..3479)
                     /locus_tag="WIB82_001850"
                     /pseudo
     CDS             complement(3292..3479)
                     /locus_tag="WIB82_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3492..3985)
                     /locus_tag="WIB82_001851"
                     /pseudo
     CDS             complement(3492..3985)
                     /locus_tag="WIB82_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
BASE COUNT          933 a         1016 c         1133 g          937 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatattataa aaattccgtc tgaagcggcg
       61 ttcagacgga atttgtttca agcaggctta acagcagcgt ccgccgtttg cgccgcagcg
      121 gcgtttgcgg caatagtctt gaaactgcag cttggtcatc acaggggcgt tgggattatg
      181 tttgcgctgc tgtgcaacgt agttttcata atcgggcacg cctgccatca agtttgccgt
      241 cagcttgatg gttttccacc aagacgcgag cttatgcttc actttgtgcc tccggctgtt
      301 tgccgtcgcg gtacaccgcc gggatttctt tggcggtcgg ccagccgact ttgcgtgctt
      361 tgagggcggt acgcaaaccg tacgcggcga caatcacgac aaccgacaag aagaggatgg
      421 tcagaccggc attaatcttg tcgttgaaga tgatttgcgc catttcgccg atgtctttgg
      481 caggcgcaag gacttcgttt ttagccaatg cgtcgctgta tttgccggcg tgggcaagga
      541 agctgatgcg cgggtcgctg tggaacagtt tttgcaggcc ggcgtagcag gttacgaaca
      601 gtacgccgac ggcgggaacg agtaccaccc agacataacg gtcgcgtttc atcttaatca
      661 gcaccacggc gcacataatc aaggccacgc ctgccagcat ttggttggcg atgccgaaca
      721 aaggccagag cgagttgatg ccgcccagcg ggtcggttac gccggtgtag aggaagtagc
      781 cccacaatac cacggcgaag aaggtcgcaa tcaggttggc ggggatggag tcggtgttgc
      841 cgaaaggttt gtagaagatg ctgcccaagt cttgaatcat aaaacgtgcg acgcgcgtac
      901 cggcatcgac ggcggtcagg atgaacaagg cttcaaacaa cagggcgaag tgataccaga
      961 acgccatcat cgcctcgccc ggaatcaggc ggctcataat gtgcgccata ccgactgcga
     1021 gggtgggcgc accgcctgca cgggaaagga tggtgttttc gcccacttcc ttagcggtgt
     1081 gcaacagggt tgccgcatcg acagggaatt gcagcttggt ggtaatcact tcggcggcgg
     1141 tattggcatc cgtaccgatc agggcggcag ggctgttcat ggcgaagtac acgccgggat
     1201 caagcgatgc ggcggcggca agcgccataa tggctacgaa actttccatc aacataccgc
     1261 cgtaaccaat catacggacg tgggtttcgt tttccagcat tttcggcgtg gtgccggagg
     1321 aaatcagcgc gtggaagccc gaaaccgcac cgcaggcgat ggtgatgaag aggaacggga
     1381 acaatgcgcc tgagaatacc ggacccgaac cgtcgataaa gtgggttacg gcaggcattt
     1441 gcaaagtggg attgacgatg acgatgccca aagccaaggc cgcaatcgta ccgattttca
     1501 ggaaggtgga gagatagtcg cgcggagtga gcagcaacca tacgggcaat acggaggcga
     1561 caaagccgta aatcataatc gcccaagtga gctggatgcc gtcaaggtcg aaccaatgtc
     1621 cgatggaact tttcgccaca tcttcgccgt aaatcacggc caacatcagc aaaataaagc
     1681 cgacgatgga aatctcgcca attttgcccg gacggatata acgcgtgtag atacccataa
     1741 acagcgcaat cggcatcgtt gcggcaatgg tgaacgtacc ccaagggctg tgaaccaatg
     1801 cttttacgac aatcaacgcc aacaccgcca taatgatgac cataatcatc aaaataccga
     1861 tggaggcaat cacgccgggg acagtgccga gttcctgttt cacaatatcg cccaaagact
     1921 taccgtcgcg gcgcatagag acgaacaaga ccatcatatc ctgtaccgcg ccggcaaata
     1981 ccacgccgaa gataatccac aaagtaccgg gcagataacc catttgcgcc gccaaaaccg
     2041 gaccaaccaa agggcccgcg ccggcaattg cggcaaagtg gtgtccgaac aatacgcctt
     2101 tgtgcgtcgg aacgtagtcc aagccgtcgt tgtggcgttc tgccggagtc aggcggtcag
     2161 gatcgagccg cattacgcgg ttggcgatgt agaggctgta aaaacggtag gcgatgcagt
     2221 aaacggatat ggcggcggtg accatccata ccgcgctgac ctgttcgcct cggctgaggg
     2281 ccagagtggt aaaggatgct aagccgacca gtaccactat gccccaaatg aggaaggttt
     2341 tgagtgattt catcgaataa atccttatct cacactgtcg gaatatgcct gaacggcggg
     2401 aaacggccga accatctgcc ggcacgggca caaaccggag tctgaatgat tgacgaagta
     2461 tgaaacagtg cgcttgtcgg gatttgtgcc ttatgcccgc atcaaacagt gcagggatgc
     2521 gggcgcacaa aacgttaaac gccgaatagg attttaacgc aaattagcac accgatagcg
     2581 gtttttactt ggaatttgga aaaaatttac attcctccgg gcgggcaggc aggttcagac
     2641 ggcatcgtca ggcaaaaggc ggcatcggaa gaggggtaaa gaaggggcgc gcaatccgga
     2701 ttattgattc atcgcaggta aattccggtt atcgggcttg tgtgtttgcg cgtccgttta
     2761 tagtatggcg ttgccgcagc ttggaatcag ggcggttgtt tcatatctta tttttattgg
     2821 ggagctttta tgaatatcag gtttttcgcg ctgaccgtac cggttttgtc tttggcggcc
     2881 tgtgccgtgc cggaggcgta tgatgacggc ggacgcgggc atatgccgcc cgttcaaaac
     2941 caagccggca cggacgattt tcgggcgttt tcctgcgaga acggtttgtc tgtgcgcgtc
     3001 cgccatttgg acagcggcaa agtcgcgttg cggctggacg gcaggcgtgc cgtcctctct
     3061 tccgacgttg ccgcatccgg cgaacgctat accgccgaac acggtttgtt cggaaacgca
     3121 accgagtggc accagaaagg cggcgaagcc tttttcggct ttaccgatgc ctacggcaat
     3181 tcggtcgaaa cttcctgccg cgcccgttaa acggggcagc ctgtttgaac cgccgtccag
     3241 atttttcggg cggttttttg tgtcggacgg acggtttttt gtgtcggatt tgttttgcag
     3301 ttcggcctcc ggcagggttc gggctgcctg ataccgtccg taacttcggg tattgccgag
     3361 ttcgtcggca acggccgctg cggcttgggc ggcgttttgc cgaacgcctg cgttacttcc
     3421 ctttgtaggt cgatctcttt ggcaaccgcg tctttgtcaa agctgttttt cagacggcct
     3481 tttgtcaaat attatcggca gtggctcaat gccaacttta aacctgctcc gatttcttca
     3541 gggctgttat ccaatgataa aatgacatcg tctgcatcaa tggcattcca cgcttccagc
     3601 ttgacatggc ggctcgggct gattttcagg cagccgttgt gcagccaaat atccacgctc
     3661 atcatgtttt taaatagggc gcgtctggtt ttatagccca agttcccgca tagcttggca
     3721 acccaatcct catagcgttg ccgaattttt tcggtatcaa aaaaatcttg gtcttctgga
     3781 ctgtcataaa cgaaagtcct gctgtttgcc aacgcttgca agactgtcgt gcctaaagtt
     3841 tcattgtcgg tatccaatgg caggatatgg ggggatatag gtggtctatt gccgttaacc
     3901 ccaaacctga acatgtttga acaatcagag tttctttatt agcatcaaaa actgcccaat
     3961 aattttgatt ctgtttaaaa atcattattt gatctccgta attttgactg tatagtgga
//
LOCUS       DAWHRS010000160         3956 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWHRS010000160 DAWHRS010000000
VERSION     DAWHRS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.160"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..406)
                     /locus_tag="WIB82_001852"
     CDS             complement(<1..406)
                     /locus_tag="WIB82_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ3843106.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKA"
     gene            complement(613..1989)
                     /gene="mpl"
                     /locus_tag="WIB82_001853"
     CDS             complement(613..1989)
                     /gene="mpl"
                     /locus_tag="WIB82_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ3843107.1"
                     /translation="MKHIHIIGIGGTFMGGLAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIDVYEGFDAAQLDEFKADVYVIGNVAKRGMDVVEAILNLGLPYISGPQWLSENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWDVAEALAPLGGRLNVGKDFDAFVAEIVKNAEVGDHILVMSNGGFGGIH
                     GKLLEALR"
     gene            complement(2051..2356)
                     /locus_tag="WIB82_001854"
     CDS             complement(2051..2356)
                     /locus_tag="WIB82_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843108.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSAAGIKPH"
     gene            complement(2368..2712)
                     /locus_tag="WIB82_001855"
     CDS             complement(2368..2712)
                     /locus_tag="WIB82_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843109.1"
                     /translation="MRIFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLSCYY
                     DEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKELDEV
                     LLKYIPFFEEER"
     gene            complement(2902..3054)
                     /locus_tag="WIB82_001856"
     CDS             complement(2902..3054)
                     /locus_tag="WIB82_001856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843110.1"
                     /translation="METHLIVQCRGGGGFGLGVVEMVLTDIAQNRANGIRTNKEFGKK
                     FVKSTN"
     gene            complement(3039..3179)
                     /locus_tag="WIB82_001857"
     CDS             complement(3039..3179)
                     /locus_tag="WIB82_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843111.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LI"
     gene            complement(3189..>3956)
                     /locus_tag="WIB82_001858"
     CDS             complement(3189..>3956)
                     /locus_tag="WIB82_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ3843112.1"
                     /translation="TNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQSGGMPV
                     GVYIPEAKMPSEEVRKLTAVLPEDGFFSVSDGILKKISSLTCADDVLALIDIPDAGAL
                     PAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVILGKGCADAWSPKVLRAGMGAHFLS
                     EIYPQADLEIWLVRYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGVGKAVLD
                     RADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
BASE COUNT          879 a         1134 c          972 g          971 t
ORIGIN      
        1 cggctttgat gattgcggaa accgtctcca cgtctttggg tttagggccg cgccacgccg
       61 cgcccataca aaaccggctt gcgccgcgcg atttggcgat tttggctttt tcgacgattt
      121 catccacatc catcatctgc tctttgccca gattggtatt gtggtgcgcc gattgcggac
      181 aataggcgca gtcttcggga caaccgccgg ttttgatgga caacagcgtg gaaagctgga
      241 tttcgcgcgg gttgaaattt tggcggtgga tttcggcggc ttggtaaatg aggtcgagga
      301 agggaagtcc gaacagggct tcgacatcgc attttttcca atagcgcgcg gtgggatgag
      361 gcttgcactc ggtcttacgg cgcaaggcga cgggggatac ggtcataatg tgttctttcg
      421 tatttacagc ggcgcaatga cgctgacagg caaaccgccg gcgcaatgac ggcggttgcg
      481 ggtaatcaaa tggctgaaaa cagcggcggc aagtgtaacg cgtttgaaaa tacggggcaa
      541 acggtttttc cgcctttgtg ccgcgcagcc gggcgatgcc gtctgaaggg ctttcagacg
      601 gcatcgcccg ggctatctca aagcttccag cagctttccg tgtattccgc cgaaaccgcc
      661 gttgctcatc accaaaatat ggtcgcctac ttcggcgttt ttcacgattt cggcaacgaa
      721 ggcatcgaag tctttgccga cgttcagcct gccgcccaaa ggcgcgaggg cttcggcgac
      781 gtcccagtcc acgccgccgg cgtagcagaa cacttggtcg gcttctttga ggcttacagg
      841 cagggcggac ttcatcgtgc ccagcttcat cgtgttggaa cgcggttcga ggacggcgag
      901 gatgcgcgcg ccgccgacgc gttggcgcaa accttgaatc gtggtttcga tggcggtcgg
      961 gtggtgggcg aagtcgtcgt aaacggtgat gccgtttgcc gtgcctttga tttccatccg
     1021 gcgtttgacg tttttaaacg cgcccaaggc ttcgcaggcg gtctgaatat cgacaccgac
     1081 atgacgcgcg gcggcaatga cggcgagcgc gttcatgcgg ttgtgcctgc ccatcaaatc
     1141 ccatttgacg cgtccggcgg ttttgccgtc gagcaacacg tcgaacgagc cgtcggcatt
     1201 ggcttcgccg gcctgccagc cgtgttccgt gccgaatttt tccaccggcg tccagcagcc
     1261 tttgtccaaa gtatcttgca ggctttgctg ccgtccgttg cagacgatta agccttcaga
     1321 cggcacggta cgcacgaggt agtggaactg ggtctgtatc gcgcccaagt cggcaaagat
     1381 gtcggcgtgg tcgaattcca gattgttcaa cacggcggta cgcggacgat aatgcacgaa
     1441 tttagaacgt ttgtcgaaaa aggcggtgtc gtattcgtcg gcttcgatga cgaaaaacgg
     1501 cgattggctg ttcgggtctt ggcgcggcgt ttgcggcagg cgggcggaaa cgccgaaatt
     1561 ttccggtacg ccgccaataa ggaagcccgg cgcgaggccg gcatattcca agacccatgc
     1621 gagcatggag gcggtggtcg ttttgccgtg cgtccccgcc acaccgagta cccaatgatg
     1681 gtgcagcacg ttttccgaca gccattgcgg gccggaaata taaggcaggc cgaggttcaa
     1741 aatcgcttca accacatcca tcccgcgctt ggcgacattg ccgataacgt aaacgtcggc
     1801 tttaaattcg tccaactgag cggcatcgaa gccttcatac acgtctatac ccaaggcttc
     1861 gagctgggtg ctcatcggcg gatacatctt cgcgtcgcaa ccgctgactt caaatcccgc
     1921 ttctttggca atggcggcaa gcccgcccat aaacgtgccg ccgataccga taatatggat
     1981 gtgtttcatc aaagttcccc aatcgttcaa attttgccgt ccgacagcgc aagggctgat
     2041 tcggcttcaa tcagtgcggc ttgatgccgg cagccgaacc gtctttctgt gcatttcctt
     2101 tcggattttg gacgtgttcg tccctcaccg tgtccgaata atcgccgacc cctgcgtctt
     2161 ccgccctgtc ttttactgcc ggtctaaccg aactgtacac ccggtcccgc cccaaacccg
     2221 cgttggtaca atgttttccc ggagtttggc tgtaactggt tttaccatta tggcggcacg
     2281 cgtaaacatt tgccgaaaat acggtctgaa ccgctgtaac aagcaacata cttccaatta
     2341 ttttagatac gcgcatatca acctccttta tcgttcttct tcaaaaaagg gaatgtattt
     2401 caatagcact tcatctaatt ctttcattcc ctgctcataa ttgaacgggt attcccaacc
     2461 atcccctact ttgatttcag aaatatcttt aaacttccgc ttaagggcgg acactttgtc
     2521 catatcgtca tcaatttcct gtttgccgaa cggtattttc cgcttttcca aaacctcgtc
     2581 ataataacaa gataaattaa gcaatagccc ccaagcacta tttgcaatta cgactaatct
     2641 atgtttttcc ctagttaagt aatctgcacc gctatcatag attgcccttg tcgggtcata
     2701 aaaaattcgc aatttttcat agcgtcccca cactttaacc gattcgcaat tttatatccg
     2761 cgattttgca agctgcccat aaaaaccgat tgcctgtcaa gacggcacta cttgttaaag
     2821 atgcccgata atgccgcaac tcttccgtat ttaccgacaa acgccctatt tgcttagagc
     2881 tgttttgttt tccacaaaac tttaattcgt tgatttaaca aattttttac caaattcttt
     2941 atttgttcgg attccattag cacgattttg agcaatatct gtcaaaacca tttccaccac
     3001 acccaagcca aaaccgccgc cgccacggca ttggacaatc agatgagttt ccactgcccg
     3061 tccttccgca atgccgcctt cttgggcttc aaagcacccg cataaaaaaa cggcgacagc
     3121 agcatagaag cgatacataa gaaaatcaat acgccgtaat agattttttc agacggcatt
     3181 tctcttcctc aatacgccgc ccgttggcgc atttgttcaa acaggcagat tgtcgccgcc
     3241 atcgcgacat ttaaagactc ggttgcatcg tgcatcggta tcctgacaca cttgtccgcc
     3301 ctatctaaaa ctgctttacc gacccccgcg ccttcgttgc caaacaccca ggctgtcggt
     3361 tcgcacaaat cttcgccgta caaaaccgcc tgcttttcct cgcgcaaggc ggtggcaaac
     3421 acacggcctt tatagcgcac caaccatatt tccaaatccg cctgcggata aatctccgac
     3481 aagaaatgcg cgcccattcc ggctcgcagc actttgggcg accacgcgtc cgcacaacct
     3541 ttgcccaaaa tgaccgcgcc gattcccgcc gccgccgcgc ttcgcaacac cgtgccgaca
     3601 ttgcccgggt cttgcacgcc gtccaaaacc acgcaatcgc cgccggccgg caaagcaccc
     3661 gcatctggaa tatcaatcag cgcaagcaca tcatccgcac aagtcaggct gctgattttc
     3721 ttcaatatgc cgtctgaaac ggaaaaaaac ccgtcttccg gcaaaaccgc cgtcaattta
     3781 cggacttctt cagacggcat tttcgcttcg ggaatatata ccccgaccgg catcccgccg
     3841 gattgcagga aaacctgaag caggtgcacg ccctccaaaa cggtttgggc gtattgccgt
     3901 ctgaactttc cttgcgacaa caggcggtgc aggtgtctga tgtgttcatt attggt
//
LOCUS       DAWHRS010000161         3916 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWHRS010000161 DAWHRS010000000
VERSION     DAWHRS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.161"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..498
                     /locus_tag="WIB82_001859"
     CDS             <1..498
                     /locus_tag="WIB82_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ3843113.1"
                     /translation="VELNPTKWDWVKNTDYKTPAARPMQTLDGEMAGGNKPPKSITSG
                     GKANAATYPQLVNQLTGQNLKNIAAQDPRLSLALHKSEKNFPIGTATYEEADRLGKIW
                     VSEGARQTSGGGWLSIDGTRQYRPPTEKNSQFATTGIQANFETYTIDSNGKRNKIKNG
                     HLNIR"
     gene            503..862
                     /locus_tag="WIB82_001860"
     CDS             503..862
                     /locus_tag="WIB82_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ3843114.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            939..1151
                     /locus_tag="WIB82_001861"
     CDS             939..1151
                     /locus_tag="WIB82_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843115.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            1248..2210
                     /gene="mafB"
                     /locus_tag="WIB82_001862"
                     /pseudo
     CDS             1248..2210
                     /gene="mafB"
                     /locus_tag="WIB82_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            2207..2578
                     /locus_tag="WIB82_001863"
     CDS             2207..2578
                     /locus_tag="WIB82_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ3843116.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            2684..3433
                     /gene="mafB"
                     /locus_tag="WIB82_001864"
                     /pseudo
     CDS             2684..3433
                     /gene="mafB"
                     /locus_tag="WIB82_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            3399..3605
                     /locus_tag="WIB82_001865"
     CDS             3399..3605
                     /locus_tag="WIB82_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843117.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            3626..3895
                     /locus_tag="WIB82_001866"
     CDS             3626..3895
                     /locus_tag="WIB82_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843118.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
BASE COUNT         1392 a          652 c          798 g         1074 t
ORIGIN      
        1 gtagaactta acccgactaa atgggattgg gttaaaaata ccgattataa aacacctgct
       61 gcccgaccta tgcagacttt agatggggaa atggccggtg ggaataaacc gcctaaatct
      121 ataacgtctg gaggaaaagc caatgctgca acttatcctc aattagttaa tcaattaact
      181 gggcaaaact taaaaaacat tgcggctcaa gatccaagat tgagtctagc tcttcataag
      241 agtgaaaaaa attttccaat aggaactgca acttatgaag aggcagatag gctaggtaaa
      301 atttgggtta gtgagggtgc aagacaaact agtggaggcg gatggttaag tatagatggc
      361 actcgacaat atcggccacc aacagaaaaa aattcacaat ttgcaactac aggtattcaa
      421 gcaaattttg aaacttatac tattgattca aatggaaaaa ggaataaaat taaaaatgga
      481 catttaaata ttaggtaatt atatgagatt aaaattaaaa aattatgatt gttctgactt
      541 tgatttggaa gatttcccac aagacaagtt agaaaatttt tgtatacttt tgacattatc
      601 aataggtttt gatgaaagta atggtgctga ttatttttat gtttatatat atagcactga
      661 atggttactt tcaaatatac atagaccgat gtcattaaaa aactcaattg ttacaaaccg
      721 ttttaatatt gaacacatat taaaattgat aaatgatata ttggaaatat gtaattcaac
      781 gtctgaagat aagtctatat caaatttggc aaaatatttt gactgggaat ttgacgatta
      841 taatctaaat atccaagact aaagatggtg ggacagcatt tagaccaaaa acaggcaaac
      901 ggtattttga tgacgaatta aaataaaagg aattattcat gaaattaatt aattttgaag
      961 agagttcagt taaaattagt ctcgataaag atgagttgta tattattcaa gcaattgtag
     1021 gagaaattta ttcaggcgta tgcgtggatt gtagagattt tgagataatt catggtgttg
     1081 aaaaaataaa gttctttctt tggataaaga acttaaaaaa atatatgata cttgggataa
     1141 atgttagcta ggtaaggtgg gcacttgttg cccacctttt gccgtatatt aaaaatcctt
     1201 ttcaggctgc ctgaaactgg gtgggcatca atgccaatcc taactgtgct gatgaagcag
     1261 gaaaactgat atgggaaaac gaccccaata aaaattggtg ggctaaccgt atggatgata
     1321 ttcgcggcat cgtccaaggt gcggttaatc cttttttaat gggttttcaa ggagtaggga
     1381 ttggggcaat tacagacagt gcggtaagcc ctgtcacaga tacagccgct cagcagactc
     1441 tacaaggtat taatgattta ggaaatttaa gtccggaagc acaacttgct gccgcgagcc
     1501 tattacagga cagtgctttt gcggtaaaag acggcatcaa ttccgccaga caatgggctg
     1561 atgcccatcc gaatataaca gcaacagccc aaactgcccc tgccgtagca gaggccgcag
     1621 gtacggtttg gcgcggtaaa aaagtagaac ttaacccgac taaatgggat tgggttaaaa
     1681 ataccggcta tgaaacaact gttacccgaa agactttaga tgggcaaata gctggcaaga
     1741 acaagcctat taaaccttct tcaggagatt atgtcttgcc ggtaggcaga caaatcatag
     1801 atcctactcg tacatctttt tcacaagcta cagttagcta tcagaagcgt ggtgcaaatt
     1861 acaactatga ttctcttgta gctgcaatga acgaaaaaaa atcatgggtt ggcgacaggg
     1921 ttgatgttgt taatatgcct gatggagcac ctactagtat ggataacacg cgtattatgg
     1981 cagcacgtga agcaggagta aaagtggaag cgaatgttca taattttaat gaccgattat
     2041 catcaaaaga gagaatcagg tttaagcatg atggtattga gcctcaaact tggggagaag
     2101 ctatccagct acgaattaga aagcaagaaa cacaaaaagg agttccagaa gggtggagca
     2161 aaagatttcc taacggaagt atttatgatg taaaggtact taggaaatga taaaacaaaa
     2221 tagttttgtt ccgtatcctg aagcaatgct tcctaaagga tttaaatatc cgcaaagtta
     2281 tttaaaatta gctcaatcca ctcatgccat taactacgat gaacaatatt cttttccttg
     2341 gtggtttgaa aatgcagaaa gcaatatatc agaagtaatt gacatttatt ttgaaataac
     2401 tggcattcca aacctattac cttttgctag aaaccaagag tgggctgcct gttttgatat
     2461 ttcagataaa tcaggtaatc ctaaaattat agtagttaat ttagataata caaaatatta
     2521 cgagactttt gaaaattttg atacttggct aaaagaagct gaaaatgatg gttggtagca
     2581 gccgtaggtc ggattctcga atccgacatt ttccaacagc ggcatttcgg aaacgataga
     2641 cgcgtcaaat atttttgtcg gatacaaata tccgacctac atctctgcgc agcaaacttt
     2701 acaagatatt aatgaattag gaaatttaag tccggaagca caacttgctg ccgcgagcct
     2761 attacaggac agtgcttttg cggtaaaaga cggcattaat tccgccagac aatgggctga
     2821 tgcccatccg aatataactg caacagccca aactgccctt tccgtagcag aagccgcaac
     2881 tacggtttgg ggcggtaaaa aagtaaacct taacccgacc aaatgggatt gggttaaaaa
     2941 taccggctat aaaacacctg ctgcccgccc tatgcagact ttagatgggg agatggcagg
     3001 tgggaataag ccaccaaaac caagtacgca gcaacaccct acacactctg ataacaatat
     3061 cggcttacct gcctcatatg ttaaacctga tacatctatt tctccgacag gaacaattca
     3121 agaccgcatc agatggacaa agtccaagtt tcctactgag aaatctttaa atggacattt
     3181 caaagctcat ggaaaagaat ttggcgatat aaccattgaa gactaccaaa aaatggcgtc
     3241 tgatttgtta tcaaaacaga catcggacaa gatattaggt tatcagacgg aacatagacg
     3301 agtgcgctat gatatcaata acaatatcta tgttttggcc aatccaaaaa cattcaaaat
     3361 caaaacaatg tttaaaccaa acttaggaaa gaagtattat gatggagaat tcaaaaaaga
     3421 catgggaaat tgacggagaa atatggctac attgtcctgt ttgcggaact gaagttatgg
     3481 actatgatat ctgtgacgtt tgtcagtggc aaaatacagg agaaactaat atagatggtg
     3541 gccctaatga aatgacactt gcggaggcga aagaagctta cgcaaaaggc ttaccaatca
     3601 gataaataag cacctagaga aatcaatgat gacggaatcc catggttacc tatcaaataa
     3661 tttacctgtt aaaatcataa atgatattat ttatgcaacc cagttagtcg aagatttggt
     3721 tttaggaaaa ataaaaattg ttgatttttt aaaatcatat aacaattttt attgttggct
     3781 tggttttgat gagttgcctc aatctgagaa aataaaattc ctaagctatc ttaatatatt
     3841 aagtattcat aaagaaatac aagatgaaac tgtgaatagg gtttataccg attgaaaaat
     3901 agtagataga gattaa
//
LOCUS       DAWHRS010000162         3887 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWHRS010000162 DAWHRS010000000
VERSION     DAWHRS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.162"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            148..1665
                     /locus_tag="WIB82_001867"
     CDS             148..1665
                     /locus_tag="WIB82_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3843119.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRLVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1724..3604)
                     /locus_tag="WIB82_001868"
     CDS             complement(1724..3604)
                     /locus_tag="WIB82_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ3843120.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFGPEELDALDGALQKGWQ
                     KGLHSVLFADYEFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDDLPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGIFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRAENGRCTLLDRHLCRLKTSAQALN
                     LPLPDGCENQIKQYIADLPDGAFRVKALLASDGISLSRAVLNRLTDKQRVIISPTILP
                     AQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKYQGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
BASE COUNT          802 a          882 c         1287 g          916 t
ORIGIN      
        1 gtgaaatccg ccctttgaaa atcgggcggc ggttttttgc ctgcccgctt tcagacggca
       61 aagccggttt tcacgggttt ctgcctgttt ttcggatggt ttgacgtgct tcggcggcgt
      121 gtttgccaga aaggtaaatg acagggtatg ttgtatttca gatacggctt tttggttgtt
      181 tggtgtgcgg caggtgtttc tgccgcctat ggggcggatg cgcccgcgat tttggatgac
      241 aaggcattgt tgcaggtgca acggttggtg tcggataagt gggcggaatc ggattggaaa
      301 gttgaaaatg atgccccgcg cgtggttgac ggggattttt tgttggcgca tccgaaaatg
      361 ttggaacata gtttgcgcga cgcgctcaac ggcaatcagg cggatttaat cgcttcgttg
      421 gcggatttgt atgccaagct gccggattat gacgcggttt tgtacggcag ggcgcgggct
      481 ttgctggcga aattggcggg aaggccggcg gaggcggtgg cgcggtatcg ggaactgcac
      541 ggggaaaatg cggcagacga gcggattttg ctggatttgg cggcggcgga gtttgacgat
      601 ttccgactga agtcggcaga aaggcatttt gcggaggcgg caaaattgga tttgccggca
      661 ccggttttgg aaaatgtggg gcgttttcgg aaaaaaacgg aggggctgac gggctggcgt
      721 ttttcgggcg gcatcagtcc ggcggtcaat agaaatgcca ataatgccgc gccgcagtat
      781 tgccggcaaa acggaggccg gcagatatgc agtgtcagcc gggcggagcg ggcggcaggc
      841 ttgaattatg aaatcgaggc ggaaaaactg acggcgttgg cagataatca ttatttgttg
      901 ttccgttcca atatcggcgg cacgagctat tatttcagta aaaaatcagc ttatgacgac
      961 gggttcggca gagcgtattt gggttggcag tataaaaatg cacggcagac ggcggggatt
     1021 ttgccgtttt atcaggtgca gttgtcgggc agcgacggct ttgatgcgaa aacaaaacgg
     1081 gtaaacaacc gccgcctgcc gccgtatatg ctggcgcacg gagtcggcgt gcagttgtcc
     1141 catacttacc gcccaaaccc gggatggcaa ttttcggtcg cgctggaaca ttaccgccaa
     1201 cgctaccgcg aacaggatag ggcggaatac aataacggca ggcaggacgg gttttatgtt
     1261 tcgtcggcaa aacgtttggg cgaatcggca actgtgttcg gcggctggca gtttgtgcgg
     1321 tttgtgccga aacgcgaaac ggtgggcggc gcggtcaata atgccgccta ccggcgcaac
     1381 ggtgtttatg ccggttgggc gcaggagtgg cggcagttgg gcggtttgaa cagtcgggtt
     1441 tctgcgtctt atgcccgccg caactataag ggcattgcgg ctttctcgac agaggcgcaa
     1501 cgcaaccgcg aatggaatgt ctcgctggct ttgagccacg acaagttgtc gtacaaaggt
     1561 atcgtgccgg cgttgaatta tcgtttcggc aggacggaaa gtaatgtgcc gtatgcgaaa
     1621 cgccgcaaca gcgaggtgtt tgtgtcggcg gattggcggt tttgaatggt gggataatgc
     1681 cgtccgaact ttggaaacag gttcggacgg catttttgcg cgttcaggca agggcggcgg
     1741 caaatacgcc gcgcagggcg ttggagaggc ggatttcttc ggcttcttgc agtgtttttt
     1801 gtgtgatgtg tgtttcgatt acttgatttg tttgcaaata tttttgcggt tcgtccaata
     1861 cggcttggcg cattatgccg tttaaaatgt ctaaatctaa agagggcgta agccattgtc
     1921 cttggtattt gacgaacacg ttgcttctgc cgccttcgag caggatgccg tctgaattga
     1981 aaaacaggct gtcgaacgcg ccttgtgttt cggcggtttg ccacgcttgg tcgaagacgg
     2041 tgcggcaggt ggttttgaag cggcgcaggt agttttgtgc gggcaggatg gtcggcgaaa
     2101 tgatgacgcg ttgtttgtcg gtcagacggt ttaaaacggc gcgggacagg ctgatgccgt
     2161 ctgaagcgag cagggctttg acgcggaacg cgccatcggg caagtcggca atgtattgtt
     2221 tgatttgatt ttcgcagccg tcgggcaggg gcaggttgag ggcttgggcg gaggttttca
     2281 gacggcatag gtggcggtcg agcagggtgc agcgtccgtt ttccgcgcgc agggtttcaa
     2341 aaatgccgaa gtcggggcgc aattcgttga ggaaacgggc tttccagccg cattcgcgat
     2401 attcggcggc ggggtcgctg tcgatgacga tgccggaacc gacaccgtac acgccccgat
     2461 acggcggatt ggagtcaaac ggatgcggcg ttgccccgcc ttgccctgcc gttcgggctt
     2521 gcgcgtttga atccgggccg ccgatgcctg aaacaatgcc gtctgaagcc gaaacgggtt
     2581 tgagcgacaa ggtgcggatg acgacgttga atatgccttc aaaccccaac ccgcccgcgc
     2641 aaggtttcag gtagccgatg ctgcccgtat aaagcccacg cgcttcggtt tcgagcgatt
     2701 cgataatctg catactcatt tttttgggcg cgccggtgat gctgccgcag gggaaggcgg
     2761 cgcggaggat gtcggcgaac gaggtgtttg gcagggcttg ggcttggatg gtgctggtca
     2821 tctgccaaac gctgccgaaa cgcgacactt taaacggctc gggcacgcat actttgccgg
     2881 tttgggcgat tttgccgagg tcgttgcgca gcaaatcgac aatcatcacg ttttcggcgc
     2941 ggtttttcgg gtcggcttgc aattcggcgg cgcggcgttc gtcttgtccg tcgcccaaaa
     3001 tcggcgcggt gcctttcatc ggttcggtgc tgatggtgcc gtctgaagcg atgttgagga
     3061 agagttcggg cgagaaacac agcgtccacg cggattgccc ctccgcatcg ggcaggtggg
     3121 acaagacggc ataggggacg ggctggcgca ggcggcggta gaggctgacg ggattgccgt
     3181 aggcttgcag gtgcaggcgg gtggtgtagt tgatttgata ggtgtcgccg cgccggatgg
     3241 cttcgtggat ttggcggatg cggtcgaggt aatcggtttc ggatacggag gattgcggcg
     3301 tggaaatgcc ggcgggaagg tcgtctgagt gtcgggcaag ccagcttgcg gcatcggtgt
     3361 cggcgcagtc ggcaaaccag tgcagggcaa ggttgccgcc gcgctcggac tcaacccccg
     3421 tcagcggcaa accgaattcg tagtctgcaa acaacaccga atgcaaccct ttttgccagc
     3481 ccttttgcag cgcgccgtcc aaagcatcga gttcttcggg accgaaaaaa cggctttcta
     3541 catgattttg atagcgtttt gcgcggccgc ttacggcatc gtcaaacagg gcgaaatagg
     3601 gcatggcaat tcggggcaaa tgttttgatt atacgctctt tttacacata ttttcagacg
     3661 attcggcaaa tatcggcaaa atgtaatttt atgtagagaa agtggggggc gaaggtgtaa
     3721 aattgtgaaa acaaattccg atttccaacc tgaaatacaa taaggagacc tttatgacag
     3781 accaccagct gcaaccgttt gaaaacgtag aattaggcga aaagcaagac cagctccaag
     3841 tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttagctc
//
LOCUS       DAWHRS010000163         3885 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWHRS010000163 DAWHRS010000000
VERSION     DAWHRS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.163"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            101..1006
                     /gene="glyQ"
                     /locus_tag="WIB82_001869"
     CDS             101..1006
                     /gene="glyQ"
                     /locus_tag="WIB82_001869"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ3843121.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1082..1492
                     /locus_tag="WIB82_001870"
     CDS             1082..1492
                     /locus_tag="WIB82_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ3843122.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQALIFGVRGSLHQAA
                     DDAEAFDRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            1513..3576
                     /locus_tag="WIB82_001871"
     CDS             1513..3576
                     /locus_tag="WIB82_001871"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ3843123.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGEVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3593..>3885
                     /locus_tag="WIB82_001872"
     CDS             3593..>3885
                     /locus_tag="WIB82_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ3843124.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWCNLDILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDE"
BASE COUNT          981 a         1128 c          998 g          778 t
ORIGIN      
        1 tgtatgccgt gttttcaaat cagtctgtct ttgagataaa cctatccaaa cagcaatgga
       61 aacagtaaaa taaacccttt attttatcga atcataagcc atgcttacct tccaacaaat
      121 catcttcaaa ctgcaaacat tctgggcaga caaaggctgc accgtcatcc aacctttcga
      181 catggaagtc ggcgcaggta cttcccaccc cgccacctgc ctgcgcgcac tcggccccga
      241 gccttggttt gccgcctacg tccaacccag ccgccgcccc aaagacggcc gctacggcga
      301 caaccccaac cgcctgcaac actattacca attccaagtc gcccttaaac ccgcccccgc
      361 caatatccaa gacctctatc tcgactccct gcgcgaattg ggcatcgacc ccaaagtcca
      421 cgacatccgc tttgtcgaag acgactggga aaaccccacc ctcggtgcat ggggtttggg
      481 ctgggaagtc tggctcaacg gcatggaagt aacccagttt acctatttcc aacaagtcgg
      541 cggcattgac tgcacgcctg tactcggcga aattacctac ggcatcgaac gcttggcgat
      601 gtacctgcaa ggcgtggaaa acgtctatga cctcgtttgg gcaaaaacgc cggacggcaa
      661 caccgtaagc tacggcgacg tgtaccacca aaacgaagtc gaacaatcca cctacaactt
      721 cgaatacagc gatgccgatt ggctgctgcg ccagttcaac gactacgaag cgcaagccaa
      781 acgcctgctt gccgaagaaa acgccgccct cgccctgcct gcctacgagc tggtcctcaa
      841 agcggggcat acgttcaacc tcttggacgc gcgcggcgcg atttccgtaa ccgaacgcgc
      901 cacctacatc gggcgcatcc gcgcgttgag ccgcgccgtg gcgcaaaaat atgtcgaaag
      961 ccgcgaaaaa ctgggctttc ccttaatgaa ggcaaatgcc gcctagggga gcgacgattt
     1021 ttacgttcgg gcggcacgat atggcagccg tccggaagcc attatatttt ttaggaaaat
     1081 tatgcctgtc tatttccgca ccccgagcag gaaacgcctg cacaaaatga accgccgcca
     1141 gcataaaaag ctgcatttgg gcgagtttca agcattgatt ttcggcgttc gaggcagttt
     1201 gcaccaagct gcggacgatg ctgaagcgtt cgaccgcttt atggacgagc tgattgcgta
     1261 tcttgaggca aatggcttgg aaatggcggg cgggggcgat tgcggcgatt tttcgtgcct
     1321 gtttcaaaaa tccggtgccc gaagcttggg cgaaaccgag aaaaaagcca ttatctcttg
     1381 gctggcaggc cgcgaagatg tcgccacttt gtacgccacc ccgctggttg atggttttta
     1441 cggggatgac ggctgggaaa attacagcga aacctataaa caggcagatt aaccaaattc
     1501 aaagaaagcc gaatgatgac ccaaaccctt ttaatcgaac tccttaccga agaactcccg
     1561 cccaaagccc tgaataatct gggcaaccat tttgccgccg ccgttgccga aggcttggaa
     1621 aaagcgcaac tggttgacgg tgcagccgaa tttacggctt atgcctcgcc gcgccgtttg
     1681 gcggttcaag tcaaaaacgt caaagccgtt caggccgacc aaaaaatcgt gaaaaaaggc
     1741 cctgccgtgg cgaatgccgt aaaagacggt gcgccgacca aggctttgga aggtttcgca
     1801 cgcggtgcag gcgcgaaaat cgaagacttg accatcgtcc acgacggcaa gcaggatgtg
     1861 tacgcctacg aatacgtcca aaccggcaag ccgctgggcg gacttttgga agacattatc
     1921 aatcaagtgg ttaagaaact gccgattccc aaagtgatgc gttggggcag cagcacattt
     1981 accttcgtgc gccctgttca cgggctgatt gtgctgcacg gcggcgacat cgtaaacgtc
     2041 agcgttttgg gtctgcaaag cagcaatcaa accttgggac accgcttctt gtccaacggc
     2101 gaaatcacca ttgaaaacgc cgacagctac gccgaacaaa tgcgcgggca aggcaaagtc
     2161 gtcgcttcgt ttgccgagcg caaagccgcg attcagacgg cattggaagg gcaggcgcgc
     2221 agtctgaacg ccgccgtcgc cgccgatgaa gcattgttgg acgaagtaac cgcgctggtc
     2281 gaatggcctg ttgttttgga agccggtttt gaggaacatt tcctcgccgt gccgcaggaa
     2341 tgcttgattt taacgatgca gcaaaaccaa aaatactttc cgctgctcga ccaaaacggc
     2401 aagctgatga accgcttcct gctggtttcc aacctgcaaa ccgaagaccc gtcgcacatc
     2461 atccgaggca acgaacgcgt tttgcgcgcg cgcctgtctg atgccgagtt cttctacaaa
     2521 caagaccaaa aagcgacttt ggaaagccgc ctgccgaaat tgtccagcgt ggtgtaccac
     2581 aacaaaatcg gctcgcaagc cgaacgcatc gaacgcctgc aaagcattgc cgcccacatc
     2641 gccaaagcat tgggcgcgga tgccgccgct gccgaacgcg ccgcgcgtct tgccaaagcc
     2701 gacttggtaa ccgaaatggt cggcgagttt cccgaactgc aaggcacgat gggcaaatac
     2761 tatgcccgct tggacggcga aaccgaagaa atcgccgaag ccgtcgagca acattaccaa
     2821 ccgcgttttg ccggcgacaa gctgcccgaa agcaaaattg ccactgccgt tgcacttgcc
     2881 gacaagctgg aaaccctggt cggcatttgg ggcatcggtc tgattccgac cggcgacaaa
     2941 gacccctacg ccctgcgccg cgctgccttg ggtattttgc gtatgctgat gcagtatggt
     3001 ttggacgtga acgaactgat tcagacggca ttcgacagct tccccaaagg tttgctcaac
     3061 gaaaaaacgc cgtctgaaac cgccgacttt atgcaggcgc gtttggccgt gttgctgcaa
     3121 aacgattatc cgcaagacat cgttgccgcc gtattggcga aacagccgcg ccgtttggac
     3181 gatttgaccg ccaaactgca agccgttgcc gcgttcaaac agctgcccga agccgccgcg
     3241 ctcgccgccg ccaacaaacg cgtgcaaaac ctgctgaaaa aagccgatgc cgagttgggc
     3301 gaagtcaatg aaagcctgct gcaacaggac gaagaaaaag ccctctttgc cgccgcgcaa
     3361 ggcttgcagc cgaaaatcgc cgccgccgtc gccgaaggca atttccaaac cgccttgtcc
     3421 gaactggctt ccgtcaaacc gcaagtcgat gccttctttg acggcgtgat ggtaatggcg
     3481 gaagatgccg ccgtaaaaca aaaccgcctg aacctgctga accgcttggc agagcaaatg
     3541 aacgcggtag ccgacatcgc gcttttgggc gagtaaccgt tgtacagtcc aaatgccgtc
     3601 tgaagccttc agacggcatc gtgcctatcg ggagaataaa ttgcagcctt tagtcagcgt
     3661 attgatttgc gcctacaacg tagaaaaata tttcgcccaa tcattagccg ccgtcgtgaa
     3721 tcaaacttgg tgcaacttgg atattttgat tgtcgatgac ggctcgacag acggtacgct
     3781 tgccattgcc aaggattttc aaaagcggga cagccgtatc aaaatccttg cacaagctca
     3841 aaattccggc ctgattccct ctttaaacat cgggctggac gaatt
//
LOCUS       DAWHRS010000164         3876 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWHRS010000164 DAWHRS010000000
VERSION     DAWHRS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.164"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(320..565)
                     /locus_tag="WIB82_001873"
     CDS             complement(320..565)
                     /locus_tag="WIB82_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843125.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            677..1105
                     /locus_tag="WIB82_001874"
     CDS             677..1105
                     /locus_tag="WIB82_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ3843126.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1117..1593
                     /locus_tag="WIB82_001875"
     CDS             1117..1593
                     /locus_tag="WIB82_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ3843127.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1943..2431)
                     /locus_tag="WIB82_001876"
     CDS             complement(1943..2431)
                     /locus_tag="WIB82_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ3843128.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2499..3554)
                     /gene="aroG"
                     /locus_tag="WIB82_001877"
     CDS             complement(2499..3554)
                     /gene="aroG"
                     /locus_tag="WIB82_001877"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ3843129.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAEHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAS"
BASE COUNT          897 a         1013 c          910 g         1056 t
ORIGIN      
        1 accctccgcc gtcattcccg cgaaagcggg aatctagaat tttaatgcgg caaaaattta
       61 tcggaaaaaa ccgaagttta aagacctaga ttcccgcctg cgcgggaatg acggtttaga
      121 aatcacccga aacgagaaaa agaaccgaaa ccgaacaagc cggattaccg cctgcgcggg
      181 aatgacggct caaaagttac gaaacgaaaa ataaccgaaa ccaaacaagt cggattcccg
      241 cgcgggcggg aatgacgata agcgtaattt caatctgcca tattgtcttt tcacttgcct
      301 gaaaacagat ttcagccacc tatccctgca tccggcggct gatgtagtcg gcaatcaaat
      361 cccgcttttc ccaaaaaaac ttctgcattt tttgttcgga atacagtttg cggaccagag
      421 tgggcaaacc gctgtggacg atggcggcga tttctttgtt gcctttttct tcctgcattg
      481 ccgtctgaaa atccataccc ctgcctttca gatattctgc aaggcggttg cggatgtggg
      541 aattgagttc gatggatttg agcattttgc ctccgttttg cggtaaggat ttattcgatt
      601 tgtttatccg cctgatttta tcacgtttcc caagcggttt gcctgtacaa taccgctttt
      661 cggcagggtg tgggcgatga tgttttcttg gttcaagctg tttcacttgt tttttgtcat
      721 ttcgtggttt gcagggctgt tttacctgcc gaggattttc gtcaatatgg cgatgattga
      781 tgtgccgcgc ggcaatcccg agtatgtgcg tctgtcgggc atggcggtgc ggctgtaccg
      841 ttttatgtcg ccgttgggct tcggcgcggt cgtgttcggc gcggcgatac cgtttgccgc
      901 cggctggtgg ggcagcggct gggtacacgt caaactgtgt ttgggcttga tgctcttggc
      961 ttaccagttg tattgcggcg tgctgctgcg ccgttttcag gattacagca atgctttttc
     1021 acaccgctgg taccgcgtgt tcaacgaaat ccccgtgctg ctgatggttg ccgcgctgta
     1081 tctggtcgtg ttcaaaccgt tttgacggag catcttatgc cgatagaaat cgaacgccgt
     1141 tttttgattg gaaacgacaa ctggcggcaa tacgccgacg aaccgctgct gttgaggcag
     1201 ggttatctgt ctgttgaaaa agaacgcacc gtccgtgtcc gcattgccgg aaaacaggcg
     1261 tggctgacgc tgaaaggcta tatttcggaa atcagccgca gtgagtttga atacgaaatc
     1321 ccgcttgccg atgcggaaaa gatgatggaa acgatgtgcc cgtttaaaat ggaaaaatgg
     1381 cgttatccgg tcagatgggg cggcagcctg tttgaaatcg atgtttttct tggcgataat
     1441 gcgcctttgg tcgtcgcgga aatcgagttg cccgacgaga atgccgattt tgaccgtccg
     1501 gactggctgg gacgggaaat cactgccgac ggtatgttta ccaatgctta tttgagcagg
     1561 catccgttct caagctggaa aaatgccgtc tgaaacggct tcagacggca ttctccgaca
     1621 accatcgcaa atccaaagac agcctctata tccttatttc tgctttcttt gttccattat
     1681 tcccaataaa tccccataac ctaaaatctc gtcattcccg cgcaggcggg aatccggttc
     1741 tttaaacttc ggttttttcc gataaattgc tttagctttt cgtttccgga ttcccgcttt
     1801 tgcgggaatg acgatggaag attgttgtta tttcggataa attaccctaa ctttaaaatc
     1861 ccggattccc atcttcgggg gaataacaat attttctatt tcagcccatc atttgcgggg
     1921 gattttatag agagtttata tttcaatatc tcaaataatg cacaaaagca tagcgcgtgc
     1981 ctttgctgct gacacggcgt tccgtccgct ctgcttcttt ccaatgcgtc cggtctattg
     2041 cggggaaaaa tgcatctcct tccacagaca aatccacttc ggttatccgc aaatcggtcg
     2101 ccaatggcat cgcttgtccg tatatctgcg cgccgcccat aatgacggct tcttccgcgc
     2161 ctgcgcacaa tgccaatgcc gcctccaaac ttgccgccgt ttccgcgcct gccgcgcaat
     2221 aatccgcctg ccggctgatg acgatgttcc tccgtccggg caggggtttg acgggcaggg
     2281 attcccacgt tttccgcccc ataatgacgg gtttgcccaa ggtataggct ttgaaaaatg
     2341 cgaaatcttc ggggatgtgc caaggcatag cattgcccgc cccgatgcac aggttttccg
     2401 cacacgccgc aattagggtt attttgagca tatttcctcc acaaaaatgc cgtctgaacg
     2461 cttcagacga cattttatga cattctgcgt caaaaatctc aactggcgcg cgccatacgt
     2521 tttttgtttg cacctgccaa caatgccaac agttcttcag tcgcgcccca accgatacac
     2581 gcatcggtaa tgctcttgcc gtacacttcc ggcttgtcct gtctgccttc gaccaaatgg
     2641 ctttccacca tcacgcccat gatattgccg ccgtcctgtt ccaattgggc ggcaatgtct
     2701 tgtgccactt ccatctgccg agtgtaatcc ttgcggctgt tggcgtggct gcaatctatc
     2761 atcagcttgt cggttacccc tgccgcacgc agttgttccg ccgcctcgct gacgtgttcc
     2821 gcatcataat tcggctcttt gccgccgcgc aaaatgacat gacagtcggg attgccgccg
     2881 gtatggacaa tggcggaatg cccggccttg gttacagaca ggaaatgatg cgaatggctc
     2941 gccgcaccga ttgcgtcgat ggcaatcttc aaattgccgt ccgtaccgtt tttaaagccg
     3001 acggggcagg acagcccgct tgccaattcg cggtgaactt ggctttcggt cgtccgcgca
     3061 ccgattgccc cccaagaaat caagtccgca taatattgcg gcgtaatcat atccaaaaac
     3121 tcggtagagg caggcatacc catattgttc agcgacaaca acaggctgcg cgcctgacgc
     3181 aaaccgaaat tgatgtcaaa cgtaccgtcc aaatgcgggt cgttaatcaa acctttccaa
     3241 cccaccgtcg tcctcggctt ctcgaaataa acgcgcatca caatcaaaag ctcgttttca
     3301 tactgcttgc ggagtttcaa caaacgctcc gcatattcca acgccgcttt cggatcgtga
     3361 atcgaacacg gcccgataat aaccaacagc cgcttgtccc tgccgtgaac caaatcggaa
     3421 atttcctgac gggtgcggtg aaccaagccc gaagcctctt tggaaatcgg cagctcgtaa
     3481 agatgggcta tcggcggcaa caactcttta acttctttaa tcttaatatc gtctgtgggg
     3541 taatggtgtg tcatcttgca tctttcctga tgggttttta tacgcggctt aggttacatc
     3601 gtttaccttg cacaaacaag tgcaacatat tactacaaag ctattttatt tcaaaataac
     3661 tgatatatct ttttttatag attttaaatt tcatattttt attattttag aaattttatt
     3721 tcaaataaaa agaaacagat tgataaaaac aatacccgcc attcatttgg acaaacctgc
     3781 acggaacatc cgttttccca tttccacgac aatccggtac agaacacccc cgctgccgat
     3841 tccgccttca catccggcaa aatttcatac acaagg
//
LOCUS       DAWHRS010000165         3859 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWHRS010000165 DAWHRS010000000
VERSION     DAWHRS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.165"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WIB82_001878"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WIB82_001878"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ3843130.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGIAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDDGGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WIB82_001879"
     CDS             1439..1924
                     /locus_tag="WIB82_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ3843131.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIB82_001880"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIB82_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ3843132.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          794 a          944 c         1069 g         1052 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcatcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaagttttag atgacggcgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgcccaa
     2041 acggcgtttc ttatcggctt cgtattcctg atagttgccg tcgaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccaac ggtcgtgaga
     2161 aatcaccatc acgctgccgg caaattccaa caatgcgtct tccagcgcgc gcagggtttc
     2221 cacatcgagg tcattagacg gttcatccag cagcaataca ttgccgccgc tcaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaagcga cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc agcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacccgccg caataatcgc ttccaaacgc ccctgctctt ccgccaacgc
     3301 gtcaaaatcc gcatcgggat tggcgtactc ggcatacact tcctccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcacgc acggtttttt ccggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aataccgccc atcggcacgg cttcgccctc
     3481 aaattcttta tccacgcccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcaaa ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg caccggcaac
     3841 catttcggat tttccaaag
//
LOCUS       DAWHRS010000166         3795 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWHRS010000166 DAWHRS010000000
VERSION     DAWHRS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.166"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            346..693
                     /locus_tag="WIB82_001881"
     CDS             346..693
                     /locus_tag="WIB82_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ3843133.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPRICI
                     PETLQTQMTHTRI"
     gene            690..3551
                     /locus_tag="WIB82_001882"
     CDS             690..3551
                     /locus_tag="WIB82_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEZ3843134.1"
                     /translation="MINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGY
                     NENYKNYTAYMRKEAPEDGDGKDIEASFDDEVVIETEAKPTDIRHVKEIGHIDVVSHI
                     IGGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFG
                     TTSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNML
                     FIFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLS
                     APYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIG
                     AVGVDSKFGWGLLDTGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKG
                     GSQLQLHGNNTYTGKTVIEGGSLVLYGNNKSDMHVETKGALIYNGAASGGSLNSDGIV
                     YLADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAG
                     YLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRG
                     NAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMP
                     GIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLD
                     HNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTW
                     SENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGT
                     LMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGL
                     AGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVA
                     GLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1026 a         1027 c         1010 g          732 t
ORIGIN      
        1 gaaccgattc acttgttaaa gaatcgttct ctttgagcta aggcgaggca acgccgtact
       61 ggtttttgtt catccactat aactagggaa attcaaatta agttagaatt atccctatga
      121 gaaaaagccg tctaagccgg tataaacaaa ataaactcat tgagctattt gtcgaaagtt
      181 caaatttcca ttttaaaaca attagtaaaa tcgagtttat cctagttgtc caagacaacc
      241 cctataataa tataattcaa aatataaaaa cgggttacat ctaaacatta cggaattttt
      301 attccctcgc ctgaattcta ttgtcagatt caaggagacc tcatcatgcg aacgacccca
      361 accttcccta caaaaacttt caaaccggct gccatggcgt tagctgttgc aacaacactt
      421 tctgcctgct taggcggcgg cggaggcggc acttctgcgc ccgacttcaa tgcaggcggt
      481 accggtatcg gcagcaacag cagggcaaca acagcgaaat cagcagcagt atcttacgcc
      541 ggtatcaaga acgaaatgtg caaagacaga agcatgctct gtgccggtcg ggatgacgtt
      601 gcggttacag acagggatgc caaaatcaat gccccccccc gaatctgcat accggagact
      661 ttacaaaccc aaatgacgca tacaagaatt tgatcaacct caaacctgca attgaagcag
      721 gctatacagg acgcggggta gaggtaggta tcgtcgacac aggcgaatcc gtcggcagca
      781 tatcctttcc cgaactgtat ggcagaaaag aacacggcta taacgaaaat tacaaaaact
      841 atacggcgta tatgcggaag gaagcgcctg aagacggaga cggtaaagac attgaagctt
      901 ctttcgacga tgaggtcgtt atagagactg aagcaaagcc gacggatatc cgccacgtaa
      961 aagaaatcgg acacatcgat gtggtctccc atattattgg cgggcgttcc gtggacggca
     1021 gacctgcagg cggtattgcg cccgatgcga cgctacacat aatgaatacg catgatggaa
     1081 ccaagaacga aataatgtct gcagccatcc gcaatgcatg ggtcaagctg ggcgaacgtg
     1141 gcgtgcgcat cgtcaataac agttttggaa caacatcgag ggcaggcact gccgaccatt
     1201 tccaaatagc caattcggag gagcagtacc gccaagcgtt gctcgcctat tccggcggtg
     1261 ataaaacaga cgagggtatc cgcctgatgc aacagagcga ttacggcaac ttgtcctacc
     1321 acatccgtaa taaaaacatg cttttcattt tttcggcaag caatgacgca caagctcagc
     1381 ccaacacact gaccctattg ccattttatg aaaaagacgc tcaaaaaggc attatcacag
     1441 ttgcaggcgt agaccgcagt ggagaaaagt tcaatggctc caaccattgc ggaattactg
     1501 ccatgtggtg cctgtcggca ccctatgaag caagcgtccg tttcacccgt acaaacccga
     1561 ttcaaattgc cggaacatcc ttttccgcac ccatcgtaac cggcacggcg gctctgctgc
     1621 tgcagaaata cccgtggatg agcaacgaca acctgcgtac cacgctgctg acaacggctc
     1681 aggacatcgg tgcagtcggc gtggacagca agttcggctg gggactgctg gatacgggta
     1741 aggccatgaa cggacccgcg tcctttccgt tcggcgactt taccgccgat acgaaaggta
     1801 catccgatat tgcctactcc ttccgtaacg acatttcagg cacgggcggc ctgatcaaaa
     1861 aaggcggcag ccaactgcaa ctgcacggca acaacaccta tacgggcaaa accgttatcg
     1921 aaggcggttc gctggtgttg tacggcaaca acaaatcgga tatgcacgtc gaaaccaaag
     1981 gtgcgctgat ttataacggg gcggcatccg gcggtagcct gaacagcgac ggcattgtct
     2041 atctggcaga taccgaccga tccggcgcaa acgaaaccgt gcacatcaaa ggcgatctgc
     2101 agctgggcgg cgaaggtacg ctgtacacac gtttgggcaa actgctgaaa gtggacggta
     2161 cggcgatgac cggcggcaag ctgtacatgt cggcacgcgg caaaggggca ggctatctca
     2221 accgtaccgg acaacgtgtt cccttcctga gtgccgccaa aatcgggcgg gattattctt
     2281 tcttcacaaa catcgaaacc gacggtggtc tgctggcttc cctcgacagc gtcgaaaaaa
     2341 cagcgggcag tgaaggcgac acgctgtcct attatgtccg tcgcggcaat gcggcacgga
     2401 ctgcttcggc agcggcacat tccgcgcccg ccggtctgaa acacgccgta gaacagggcg
     2461 gcagcaatct ggaaaacctg atggtcgaac tggatgcctc cgaatcatcc gcaacacccg
     2521 agacggttga aactgcggcc gccgaccgca cagatatgcc gggcatccgc ccctacggcg
     2581 caactttccg cgcagcggca gccgtacagc atgcgaatgc cgccgacggt gtacgcatct
     2641 tcaacagtct cgccgctacc gtctatgccg acagtaccgc cgcccatgcc gatatgcagg
     2701 gacgccggct gaaagccgta tcggacgggt tggaccacaa cgctacgggt ctgcgcgtca
     2761 tcgcgcaaac ccaacaggac ggtggaacgt gggaacaggg cggtgttgaa ggcaaaatgc
     2821 gcggcagtac ccaaaccgtc ggcattgccg cgaaaaccgg cgaaaatacg acagcagccg
     2881 ccacactggg catgggacac agcacatgga gcgaaaacag tgcaaatgca aaaaccgaca
     2941 gcattagtct gtttgcaggc atacggcacg atgcgggcga tatcggctat ctcaaaggcc
     3001 tgttctccta cggacgctac aaaaacagca tcagccgcag caccggtgcg gacgaacatg
     3061 cggaaggcag cgtcaacggc acgctgatgc agctgggcgc actgggcggt gtcaacgttc
     3121 cgtttgccgc aacgggagat ttgacggtcg aaggcggtct gcgctacgac ctgctcaaac
     3181 aggatgcatt cgccgaaaaa ggcagtgctt tgggctggag cggcaacagc ctcactgaag
     3241 gcacactggt cggactcgcg ggtctgaagc tgtcgcaacc cttgagcgat aaagccgtcc
     3301 tgtttgcaac ggcgggcgtg gaacgcgacc tgaacggacg cgactacacg gtaacgggcg
     3361 gctttaccgg cgcgactgca gcaaccggca agacgggggc acgcaatatg ccgcacaccc
     3421 gcctggttgc cggtctgggc gcggatgtcg aattcggcaa cggctggaac ggcttggcac
     3481 gttacagcta cgccggttcc aaacagtacg gcaaccacag cggacgagtc ggcgtaggct
     3541 accggttctg acggacagga agcagacagc cgcaaagatc acggtctttg cggctgtttc
     3601 ttatgaaaag aaaaccctat tccaattgcc tgcttctatt gtttcaagac ttcttccaaa
     3661 gattcggcat taatcagatg tatagcggat taacaaaaat caggacaagg cggcgaagcc
     3721 gcggacagta caaatagtac ggaaccgatt cactcggtgc ttgagcacct tagagaatcg
     3781 tttctttgag ctaag
//
LOCUS       DAWHRS010000167         3786 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWHRS010000167 DAWHRS010000000
VERSION     DAWHRS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.167"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..217
                     /locus_tag="WIB82_001883"
     CDS             <1..217
                     /locus_tag="WIB82_001883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843135.1"
                     /translation="SLHIENAAQANLQFARLVLALSHGERGLGCRLPLRFRQILTSLI
                     LRFSFAEAALSDDLLEHHRYTHLRNAV"
     gene            complement(228..551)
                     /locus_tag="WIB82_001884"
     CDS             complement(228..551)
                     /locus_tag="WIB82_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ3843136.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(631..1287)
                     /locus_tag="WIB82_001885"
     CDS             complement(631..1287)
                     /locus_tag="WIB82_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ3843137.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1390..1917)
                     /locus_tag="WIB82_001886"
     CDS             complement(1390..1917)
                     /locus_tag="WIB82_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ3843138.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADEADWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGET
                     VVVKDVNAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAV"
     gene            2072..2845
                     /locus_tag="WIB82_001887"
     CDS             2072..2845
                     /locus_tag="WIB82_001887"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ3843139.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRIHPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2852..3202
                     /gene="secG"
                     /locus_tag="WIB82_001888"
     CDS             2852..3202
                     /gene="secG"
                     /locus_tag="WIB82_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ3843140.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3216..3301
                     /locus_tag="WIB82_001889"
     tRNA            3216..3301
                     /locus_tag="WIB82_001889"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3250..3252,aa:Leu,seq:gag)
BASE COUNT         1039 a         1183 c          809 g          755 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaaccttcag tttgcccgcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg atactgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcaggc tgcggatacc gtgatggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgctttca attccgcggg cccaagttgc
      541 acaatatcca tcgcaccccc caaaaaaaac caagcaaaat gccgtctgaa gccccaaacc
      601 cgctttcaga cggcatgacc tgtcaacatc ttaaaaatcg aaaccgccaa acggatcggc
      661 atccttatca tccaaatgcg ccaccaaggt atcgaacagc accttctctt caaacacatc
      721 gcccctgcgc gtaatcaaaa gcgcgcgttg aacaggcttg cgacctacga taaccaccat
      781 gcgtccgcca tctttcaact gttctttcaa cacttcaggc acaaggttta ccgcaccgcc
      841 gacataaacc gcatcaaacg gcgcacctgc ggaaagttcg gtcaacccgt tgttttgcac
      901 ataatcgata ttgtccaaac ccaagccgtc caacaccgct ttggcgcggt tttgctgttc
      961 ggcatcgatg tcgtccgaca ccacacgacc agccaatttt gccaacagcg cggtcgcata
     1021 gcccgaaccc gtgccgattt ccaaaaccgt atcgtttttc gtcagcttca agccctgcgc
     1081 cagccgcgcc acgactttcg gctcgagcat cttatgaccg ttggcaagcg gcagcgccat
     1141 atccgcatac gccaaaccct gcaagtcctc atcgacaaaa agctcgcgcg gaatctccgc
     1201 caaagcgtcc aacacatcaa aatccaatac atcccacgga cggatttgct gttcgaccat
     1261 attgaaccgc gctttttcaa aatccattta gtgctccgtc aaataattgt tccaacaatg
     1321 gcaggcatta taaaaccaca ctcccgccgc gccaacttcg gcacgcgccg acagcccgtt
     1381 ttgtcagtct caaaccgcct gacgcgaagc ctcaaaccgc ttctccaaaa tcttcgccag
     1441 ttcgcccaaa tacaaagcat ccgtctcatc aaactgcgcc aaatgttcgc tgtccgcgtc
     1501 caacacgccg atacagcggc cgtctgaaaa cagcggcacg acaatctccg aacgtgacaa
     1561 agacgaacag gcaatatggt cgggatgcgc gttgacatcc ttaacaacca ccgtttcacc
     1621 cttcgcccaa gcctgaccgc acaccccgcg accgaacgga atccgcgtac acgccaaagg
     1681 cccctgaaac ggtgccaaaa ccaattcgtc cgaacgcgta tcgaccaaat aaaaacccac
     1741 ccaaaaccaa ccgaacgcct ccttcaaaac cgccgccgtg ttcgccaaat tcgccaccca
     1801 atccgcctcg tcagccacca cagactcaat ctgcggcaac acctcccgat aaagcgcggc
     1861 cttgtccgaa gccgaaaaat gaagcgcgtg catcacatct cctatagttg catacatatc
     1921 aggcggccat tataaaacag cctgcccgaa acaacattcc aaaccgcccg gccggccgct
     1981 tcaagttgcg aacccgccgc atatccacta aacttcacgt tgcaccgcgc cacacgcggc
     2041 agacaaaaaa acacgacacg gagcaaaaaa gatgtatcgc caaatcggaa tgtgggatca
     2101 aaaatgggtc atcggcaact ggaaaatgaa cggccggctc caaaacaaca acgcactgat
     2161 gcaccgcttc cgcatccacc ccaccgccga acgcgtcctc atcggactcg ccgccccgac
     2221 cgtttacctg ctgcaactgc acaacgccat gcaaatcgtt ttaaacaacc gcatcctcac
     2281 ctgcgcccaa gacgtgagcc gcttccccga caacggcgcg tacaccggcg aagtgtccgc
     2341 cgaaatgctc gccgacaccg gcacagacat cgtcctcatc ggacactccg aacgcagcct
     2401 ttatttcggc gaaaaaaacg aaatccaacg ccgcaaaatg gaaaacgtcc tcaacgtcgg
     2461 actcatcccg ttattgtgcg tcggcgaaag cctcgaagag cgcgaagccg gcaaagaaca
     2521 cgaagtcatc gcccatcagc tttccatcct gcaagggctg gataccaaaa acatcgccgt
     2581 cgcctacgaa cccgtctggg cgatcggcac cggcaaagtc gccaccgtcg aacagattgc
     2641 cgatatgcac gcattcatct acaaagaaat cttgtctttg tgcggaagcg atgttaaaat
     2701 ccgcgtcctt tacggcggaa gtgtgaaagc ggacaacgcg gccgacatct tcgcagtacc
     2761 ttatgtggac ggcgcactcg tcggcggcgc gtcattgtcg tacgactcct ttaccgccat
     2821 catcagtgcc gcacaaaatg cgtagaaaaa tatggaagcc ttcaaaaccc taatttggat
     2881 tgttaatata atttccgctt tggccgtcat cgtgttagta ttgctccaac acggcaaagg
     2941 cgcggatgcc ggcgcgactt tcggatcggg aagcggcagc gcgcaaggcg tattcggctc
     3001 tgccggcaac gctaacttcc tcagccgctc gaccgccgtt gcagcaacat ttttctttgc
     3061 aacctgcatg gctatggtgt atattcacac ccacacgaca aaacacggtt tggacttcag
     3121 caacgtacaa caaactcagc aagcacccaa acccgtaagc aataccgaac cttctgcccc
     3181 tgttcctcag cagcagaaat aacagttttt caaatgccga catggtgaaa ttggtagaca
     3241 cgctatcttg agggggtagt ggccgtaggc tgtgcgagtt caaatctcgc tgtcggcacc
     3301 aacacacaaa aacgcctgaa aatttcaggc gttttttgtt caatcccatc caacgctgtc
     3361 aaccaattcc aatcaataca aggatttcag gctgattgtt atcctgccgt cccccttcct
     3421 gacagtgcaa tcccgtccaa tccgccctaa ttgaagtaac ctaaaattta cggtatcttt
     3481 tgcggtatct gaaaaatacc tcgaaaaaat accgcaaaaa taaagctgaa cgaccgccaa
     3541 atcaggaatg ccaagcggaa aagagcttgc ggggaatact gccaagacgt agggaacaag
     3601 ggggaaaccg tccaagatgc agggcggttt tttttgggtt tttggaaaaa acctatacta
     3661 ggaagcgata cccttagttg ttaccttgtt accggggaaa agttagataa ataagcatat
     3721 gaaatatagt gaattaaatt taaatcagga caaggcggcg agccgcagac agtacaaata
     3781 gtacgg
//
LOCUS       DAWHRS010000168         3705 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWHRS010000168 DAWHRS010000000
VERSION     DAWHRS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.168"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            42..1250
                     /locus_tag="WIB82_001890"
     CDS             42..1250
                     /locus_tag="WIB82_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ3843141.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
     gene            1271..1582
                     /locus_tag="WIB82_001891"
     CDS             1271..1582
                     /locus_tag="WIB82_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843142.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            1586..1786
                     /locus_tag="WIB82_001892"
     CDS             1586..1786
                     /locus_tag="WIB82_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843143.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            2013..2243
                     /locus_tag="WIB82_001893"
     CDS             2013..2243
                     /locus_tag="WIB82_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843144.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDASAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVR"
     gene            2271..2570
                     /locus_tag="WIB82_001894"
     CDS             2271..2570
                     /locus_tag="WIB82_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843145.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGEKW
                     ILNGKPVTLSYPKCSNYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
     gene            2689..3003
                     /locus_tag="WIB82_001895"
     CDS             2689..3003
                     /locus_tag="WIB82_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ3843146.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYYGKTMAYTFASELLDYSKVNKF
                     IIHEEIQCFLNRRISNNIWKIYFSDESVAYIKILELQDDSSRGIEIKTFDFNPNVGDV
                     FG"
     gene            2981..>3705
                     /locus_tag="WIB82_001896"
     CDS             2981..>3705
                     /locus_tag="WIB82_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3843147.1"
                     /translation="MLGMFSVNSYAERSKYHIGNSNVRLDIDHTKSVVTDFRVDGQRF
                     SGRIIEPSIIEHVPTGARSLEKVPVKFTASVSRAAVLSGVGKLVRQGAKFSTRAVPYV
                     GTALLAHDVYETFKEDIQARGYQYDTETDKFVKGYEYSNCLWYEDERRINRTYGCYGV
                     DSSIMRLMSDYSRFPEVKELMESRMERLARPYWEKLRNRPDMYYFKNYNFKRCYFGLN
                     GGDCLVAKGDDGRTFISFSLQGNS"
BASE COUNT         1155 a          628 c          838 g         1084 t
ORIGIN      
        1 ggggagcgaa ataaaacaaa tccctaaagg tactgaacaa aatgagtgaa gcagaatatt
       61 tttcccattt catatctaac ggcaatggga agttattaga aattccgcaa cgtaggggca
      121 ggcaggacgg cgtttttata gattggttgt cattcacact gcacgaagat tccttgctga
      181 aagtttccgg atgcccctta gtttccgatg ccgaatatat gtttgtttta agcaaaaaat
      241 tggaggaaat attagggttt ggcatcacga gcagatgcaa atcgaagggc aataaatttt
      301 acgattcgat gtttaggttg ggatcggaag aagttgacta cggcgaagtc cattacggag
      361 gtcagcgaaa tacggtttta atcgaattga aaggtgtagg ttgcaacatt gcaaatccag
      421 gttgggaatt gaggcttaag cagtttttgg aagattcatt gaggccgagg ataacgcggg
      481 tagatttggc acttgatttt tttgatgggg agtacacgcc ggaacaggca cttttggatc
      541 acgataacgg ttttttcgat aacagtaaca tgaggccgaa atctgaaatg gttggaacgg
      601 cttggcggag agaggacggg agcggcaaga cattttatgt aggtcgcaag aaaaattctc
      661 gttttgtgcg tgtttatgag aaaggcaggc agctaggcga taaagaaagc aagtgggtaa
      721 ggttcgaaat tcagtttaat catggagata tggaaatacc tttggatatt ctgataaatc
      781 aaggttctta cttttcaggc gctttcccga tttgtcagaa atttaaaaat atgccgaatc
      841 cggaaaggtt cgattaccgt aaaaaagtgg ctaatttaac ttttcagcat aaattgagat
      901 acgcaaaaaa cgcggtcggc aaactgatta atttcatgtt tgatatgggt tttgatagtg
      961 atgaaattgt cagatatctg aaggcagatt tggggtatcc caaagggcta gaacctgaaa
     1021 aatattcgtt ggccggattg aaggaatctt tgaaattcgg ctttatccac gaacaaccgg
     1081 atgtagattt agaggttgaa ttggaagaac tcggaattat caaatttaag caatcagata
     1141 aattcgatcc ggataaaagg cttttcgatc cacatcacga tgtagaaagt gagaggcaat
     1201 atcagcttta tctcgacaga atgtatgatc ttcatgcaaa tcaaaattaa cctaaaaagg
     1261 aaaaattaat atgtttaatc aaactcaaac tgtaacttat cccgcaactt ttttaggagc
     1321 taaaaaattc aaaggcgaaa ttgatggctc taatatcgac acttgttccg tattggttgc
     1381 aacacctttg ccggcacagt cgggaaatgc tgttggattc acggcagcac aaatgaagtt
     1441 cggggacagt aagaatttct caaaattaga gaatctcaaa tacccgtgcg aagttatggt
     1501 aacggttgaa atgacttcga caggtaaggg catggttcct tcattaattg attttcaggt
     1561 ggcagaaaag ccgaaaggtt gatttatgaa atttgaagaa cgtttcatag ttcaagattt
     1621 agaaacgcat gactttattt atcccgatcc gttcggtgac gtggggttta ctcaaaatat
     1681 taaatcagca ggtcaatttg aaagttacga agatgcattg aactcaggca taaatgaaat
     1741 aggcggagga ttccaaatat ttcagttctt cgtgaaatcg gaataaaagt aaaacaggct
     1801 cggcgggcgg tctgtcaacc tttcacaaag cccgcaaaaa ggattaaaaa tgaataaagc
     1861 aactttaatt atcggatttt ttgtttgcct cattcttttc attttctcaa ttctctattt
     1921 cataaattaa aaacaaaatt cgggagcagg cttcgcccgg atttaaacct aagaagccgt
     1981 attactttaa atatcaaaaa aggaaaaaaa cgatgaacat cgttaaaaaa tacgctgtaa
     2041 aagcagcctt ggcagccggt atcttcacac cggccattgt tatggcagat acctttgatg
     2101 catccgcgat tggtacgcaa gtagcgaatg taatcatggg tttcgtgtca atggtttccg
     2161 ccgtgggtat ggcggccatt accgtgattc ttgcaatcca aggcttcaaa atggcttgga
     2221 gcatgattaa atctgtcaga taaacagagt gaagaaaaag gggcgcataa atgggctatc
     2281 gtgtcggcat aaattgtttt gatacaagat tgcaggcaga cgactattta ttgtcgtccc
     2341 ttcctcctac tgttacccag gacggaaaaa tcatcaggcc ggaaagggtg ggcgaaaaat
     2401 ggattttgaa cggaaagccg gtcacgctgt cttatccgaa atgttcgaat tacgaacaag
     2461 ttaaatccgg agcttatctc gggtctatgg ttttaattct gtttgtcgtt atttacggct
     2521 tcaggctgct gattaatttc ttaaaagaca taggcaaagt aggggcgtga tgatgtttgt
     2581 cgatttttgg tttttgctag gtttcttctt ggctttgtct gtcgctttga tatttaaatg
     2641 acgtgtttta aaatcaggct ttcaaaacaa cctttgaaag gcagaacaat gaacaaaccg
     2701 tttatcactc aggcgcagtt ggcactttat aaatatcagc cgtctagcaa gtattatggt
     2761 aaaacaatgg catatacttt cgctagtgag cttttggatt attcaaaagt taataaattt
     2821 ataattcatg aagaaatcca atgtttttta aatagaagga tttctaataa tatttggaaa
     2881 atttattttt ctgatgagtc tgttgcgtat ataaaaattt tagaattaca ggatgattct
     2941 agtcgtggaa ttgaaattaa aacgtttgat tttaatccta atgttgggga tgttttcggt
     3001 taattcttat gccgaacgtt ctaagtatca cattggaaat tcaaatgtta gattggatat
     3061 tgatcataca aaatctgtag ttaccgattt tcgtgttgat ggtcagcgtt tttcaggtcg
     3121 aattatcgaa ccttcaataa tagaacacgt gccaacaggt gcacgctctc ttgaaaaagt
     3181 ccccgttaaa tttaccgcat cagtttcccg cgccgccgtc ttgtcaggag tcggcaaact
     3241 tgtccgccaa ggcgcgaaat tcagcacaag ggcagtccct tatgtaggga cagccctttt
     3301 agcccacgac gtatacgaaa ctttcaaaga agacatacag gcacgaggct accaatacga
     3361 caccgaaacc gacaaatttg taaaaggcta cgaatatagt aattgccttt ggtacgaaga
     3421 cgaaagacgt attaatagaa cctatggctg ctacggcgtt gacagttcca ttatgcgcct
     3481 tatgtccgat tacagcagat tccccgaagt caaagaattg atggaaagcc gaatggaaag
     3541 gcttgcccgt ccgtattggg aaaagttaag gaatcgtcct gatatgtatt attttaaaaa
     3601 ctacaatttt aaacgttgtt atttcggatt gaatggcgga gattgtttag ttgctaaagg
     3661 tgatgatggt agaactttta tcagtttctc acttcaagga aattc
//
LOCUS       DAWHRS010000169         3705 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWHRS010000169 DAWHRS010000000
VERSION     DAWHRS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.169"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..707)
                     /gene="argB"
                     /locus_tag="WIB82_001897"
     CDS             complement(<1..707)
                     /gene="argB"
                     /locus_tag="WIB82_001897"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ3843148.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRI"
     gene            877..2007
                     /locus_tag="WIB82_001898"
     CDS             877..2007
                     /locus_tag="WIB82_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ3843149.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2078..2929
                     /locus_tag="WIB82_001899"
     CDS             2078..2929
                     /locus_tag="WIB82_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ3843150.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3012..3674)
                     /locus_tag="WIB82_001900"
     CDS             complement(3012..3674)
                     /locus_tag="WIB82_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ3843151.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          797 a         1083 c          913 g          912 t
ORIGIN      
        1 tcaatccgtt tcggcgtgag tttggtcagc agattgcccg ttttgtccat cacaccggcg
       61 atattcgtca tcatcaagag tttttcggcg ttcaattctt ccgccaattt gcctgctacc
      121 aaatcggcgt tgatgttgaa cgcttcgcct ttttcaccta cgccgacggg ggcgacgacg
      181 ggaatgcagc cacgttctat cagcccttta accaaacctg tatcgatgct ttccaccgta
      241 ccgacctgtc cgatgtctac gccattctgt tcgggcgtat cgatcaaaag tttcttcgcc
      301 ttaatgaaat ggtcgtcgcg tccgctcacg ccgaccgcgt gcccgccata tgtgttaatc
      361 atcgacacga tttctttatt gacgtgcccg cccaacacca tttcgacaat atccatcgcc
      421 tctttgtcgg taacgcgcat tccttggaca aactcgccct ttttgccgac tttttcaagc
      481 atcgcattga tctgcggccc gccgccatga acgatgacgg gatgaatgcc gaccagcttc
      541 agcagcacga catcgcgggc aaacccttct ttcaaggcag gttcggtcat cgcgttgccg
      601 ccgtatttga tgacggcgac cgaaccggaa aaccggcgga tgtaaggcag cgcttcggca
      661 aggatacgcg ctttgtcggc ggcggaaata atgttttcag actccataac gactccctat
      721 tgttgacaat ttcggcttat gttaacgcca aacgccgtct gaaacaatct tcagacggca
      781 tccggcagac cttcgggcag gttgaagcct tgcgatacgg gcggtaaaat cgcggtttat
      841 ccggttcgag aaaccggcga cagcaaggac tttcccatga ttaaaatcag cacccgcttc
      901 gatgccggct cggtcgtcgt caaagacctg accgaccctt ccaacatccg cctcgccctg
      961 cgtcccgaca acgcctccga ttttgcacaa tggttctact tccggctgca aggcgcggca
     1021 tatcaaaact gcatcatgca ttttgaaaac gcggcagaag ccgcctaccc gaaaggctgg
     1081 gaaggctatc aggcgtgtgc ctcatacgac cgccgcaact ggttccgcgt accgacttcc
     1141 tacgaaaacg gcgtactgac cgtcaatcat acgccgctgt ccaacagcgt gtattacgcc
     1201 tatttcgaac cttactccga agaacagcat ttaaacctcc tcggcgacgc gcaaggcagc
     1261 ggcctgtgcc gcatcgacga cttgggcagc accgtgcaag ggcgcgacat caatctgctg
     1321 accatcggca atcaggtcga aagcgatctg aaaatctgga ttaccgcccg ccagcatccg
     1381 ggcgaaacca tggcggaatg gtttgtcgag gggctgctcg gcaggctgct cgatccgcaa
     1441 gacccgacag cgcgcaccct gctcgaccgc gccacgttct atatcgtccc caatatgaac
     1501 cccgacggtt cggcactggg caaccttcgt accaacgccg ccggtgcgaa cctcaaccgc
     1561 gaatgggaaa atccgacttt ggaaaaaagc cccgaagtgt tcttcgtgcg tgaaagaatg
     1621 ctggaaaccg gcgtggattt gtttttggat atccacggcg acgaagggtt gccctttgtc
     1681 tttgttgcag gaactgaagg cgtgccgaac tacaatccgc gcatttccgc gttggaaaca
     1741 cagttcaaaa ccgccctttt gaacgccagc cccgatttcc aagatgaata cggttacgaa
     1801 aaagatgcgc ccggtcaggc aaacgtgacc ttggcaacaa actgggtcgg caaccgtttc
     1861 aactgccttg cctatacgtt ggaaatgccg ttcaaagaca atgccaacct gcccgacgac
     1921 gacttcgggt ggaacggtca acgttcgttg cgcctgggcg aagccgcgtt gtccgccatc
     1981 ctcaacgtca tcggcgattt gcgctgaaac ccttattgca gaaatgccgt ctgaagcatc
     2041 atgcctttca gacggcattt gaaaacgaaa gcctgttatg attatccatc cccaattcga
     2101 tcccgtcctc atcagtatcg gcccgcttgc cgtccgctgg tatgccctaa gctacatcct
     2161 cggatttatt ctttttacct ttctcggcag aaggcgcatc gcgcaaggct tgtccgtttt
     2221 taccaaagaa tcgcttgacg acttcctgac atggggcatt ttgggcgtaa tttttggtgg
     2281 gcgtttgggt tacgtcctgt tttacaagtt ttccgactac ctcgcccatc cgcttgatat
     2341 tttcaaggta tgggaaggcg gaatgtcgtt ccacggcggc tttttgggtg tagttattgc
     2401 catatggttg ttcggccgca aacacggcat cggcttcctc aaactgatgg acacggtcgc
     2461 accgctcgtt ccgctgggtc tcgcttcggg acgtatcggc aacttcatca acggcgaact
     2521 ttggggacgc gttaccgaca tcaacgcatt ttgggcaatg ggcttcccgc aggcgcgtta
     2581 cgaagacctc gaagccgccg cgcacaatcc gctttgggca gaatggctgc aacaatacgg
     2641 tatgctgccg cgtcatccct cgcagcttta tcagtttgca cttgaaggca tctgcctgtt
     2701 cgccgtcgtt tggctgttct ctaaaaaaca gcggccgacc ggacaagtcg cctcgctctt
     2761 cctcggcggc tacggcatat tccgcttcat tgccgaattt gcacgccaac ccgacgacta
     2821 tctcgggctg ctgaccttgg ggctgtcgat ggggcaatgg ttgagcgtcc cgatgattgt
     2881 tttgggtatc gtcggctttg tccggttcgg catgaaaaaa cagcactgac cgcctgtgaa
     2941 aaataccgtc tgaaacatgg aaatccggtt tcagacggca tttttacgtt tggttcatgg
     3001 aaacaaacgc ttcaattctt accgcaacac gccttgtatt ttctgccgct gccgcacgga
     3061 cagggatcgt tcctgcctgt tttttcgcct tccctgcgga cggtttgcgg cttgttgatg
     3121 accgcctgcc agtagcggta gatatccgcc aatgcataag gcaactcgga ttccagttcc
     3181 gccagctcgc cttctgtgaa ttgcagacgg atagtgccgt tgtcctcttc gtcgtaaata
     3241 ccgcccaatg ccatgatggg ataaaacaac tcttcaaacg cttcatcatc gacggcttca
     3301 aaccaatcgg tcggcacaat atccaaaccg taaagataag cattgcacca tgtgtaaaaa
     3361 tcgctgccgc cgtcttcgtt ttcatacagc cacaaatcgg gcagtttttt atccgacatc
     3421 gcggcggttg tttccatcgc cattgccaaa accagccgtt cgatttcgga acgttcggcg
     3481 gcggtaaatt gcgattcgtc gcccaacact tcgggcagcc agtcgagcgg tgtcaatttg
     3541 tccggcccgc tcaacagcgc cgtcataaaa ccttgaacct cgtcgcaacg catcgtgttg
     3601 ccttgttcgc ttttggcatc caacaattcg ctcaaccgcc gtttggatgc ttcggtaaat
     3661 ttttgggaat ccatcatttt ccttttcaaa tgggttttgc gccct
//
LOCUS       DAWHRS010000170         3671 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWHRS010000170 DAWHRS010000000
VERSION     DAWHRS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.170"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            155..328
                     /locus_tag="WIB82_001901"
     CDS             155..328
                     /locus_tag="WIB82_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ3843152.1"
                     /translation="MKSERRHWEWCVDYDEEGVVRDYRFTHKVGKDERSVIRDTVDVI
                     RKEAGKSLSQPEK"
     gene            392..1675
                     /gene="hemL"
                     /locus_tag="WIB82_001902"
     CDS             392..1675
                     /gene="hemL"
                     /locus_tag="WIB82_001902"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ3843153.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIAIAEQIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTALTSRLADG
                     IAAAAKAHGIEFTADSVGGMFGLYFASHTPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELINETVAVAVEVFKAMAE"
     gene            complement(1891..2163)
                     /locus_tag="WIB82_001903"
     CDS             complement(1891..2163)
                     /locus_tag="WIB82_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ3843154.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2174..2737)
                     /gene="orn"
                     /locus_tag="WIB82_001904"
     CDS             complement(2174..2737)
                     /gene="orn"
                     /locus_tag="WIB82_001904"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ3843155.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2755..3642)
                     /gene="prmA"
                     /locus_tag="WIB82_001905"
     CDS             complement(2755..3642)
                     /gene="prmA"
                     /locus_tag="WIB82_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ3843156.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGTKR"
BASE COUNT          770 a          962 c         1047 g          892 t
ORIGIN      
        1 gacggaagag ggaatcgcac aaacggttgt taaaggcaaa acgacccggg cggaggtaga
       61 ggcacgtttc gggcggcaaa atccgttcgg ttgttatgcc tatcatgaag tcagcctgcc
      121 tatttataat tttttaccga ccaatttcat ttatataaaa tcagaacgcc gacattggga
      181 gtggtgtgtg gattacgacg aggagggagt tgtccgggac taccgcttta cacataaagt
      241 gggaaaagac gagcgttccg tcatccggga tacggttgac gtcatccgca aagaagcggg
      301 caaatccttg tcgcaacctg aaaaatgata aaatggggct ttctgcttcc aagcccgaaa
      361 cctgccgttc agacggcatt tgaggataaa tatgaaccgt aacgaaattt tattcgaccg
      421 cgccaaagcc atcatccccg gcggcgtgaa ttcccccgtc cgcgcattcg gcagcgtcgg
      481 cggtgtgccg cgctttatca aaaaagccga aggcgcatat gtttgggacg aaaacggcac
      541 gcgctacacc gattacgtcg gctcttgggg tcctgcgatt gtcggacacg cacaccccga
      601 agtcatcgaa gccgtgcgcg aagctgcctt gggcggtttg tcgttcggcg cgcccaccga
      661 gggcgaaatc gccattgccg aacaaatcgc caaaatcatg ccgtccgtcg aacggctgcg
      721 cctcgtcagc tccggtaccg aagcgacgat gaccgccatc cgtttggcac gcggttttac
      781 cggtcgcgac aaaatcatca agtttgaagg ctgctaccac gggcattccg acagcctgct
      841 ggtgaaagcc ggcagcggcc tgctgacgtt cggcaatcct tcttccgccg gtgtgcctgc
      901 cgactttacc aaacacactt tagtccttga gtacaacaac atcgcccaat tggaagaagc
      961 ctttgctcaa agcggcaatg acattgcctg tgtgattttg gagccttttg tcggcaatat
     1021 gaaccttgtc cgcccgaccg aggcctttgt caaagccttg cgcggattga ccgaaaaaca
     1081 cggcgcggtg ttgatttacg acgaagtgat gaccggcttc cgcgtcgcgc tcggcggcgc
     1141 gcaatcgctg cacggcatca cgcccgacct gaccacgatg ggcaaagtca tcggcggcgg
     1201 tatgccgctt gccgcgttcg gcggacgcaa agacatcatg gaatgtattt ccccgctggg
     1261 cggcgtgtat caggcaggca cattatcagg caacccgatt gccgtcgccg ccggcttgaa
     1321 aacgctggaa atcatccggc gcgaaggctt ctatgaaaac ctgactgccc tgaccagccg
     1381 cctcgccgac ggtattgccg ccgccgccaa agcgcacggt atcgaattca ccgccgacag
     1441 cgtgggcggt atgttcggtc tgtattttgc cagccatacg ccgcgaaact atgccgatat
     1501 ggcgcgatcc aatatcgacg ctttcaaacg cttcttccac ggcatgctcg accgcggcat
     1561 tgcctttggc ccgtccgctt atgaagcagg tttcgtttcc gccgcgcata cgcccgagct
     1621 gattaacgaa acggttgcgg ttgcggttga agtgttcaag gcgatggctg aatgatgttt
     1681 tgacggacag agtttctctg ttcgatttgt ttggcagatt gaagtaagaa tgcacaccgc
     1741 cgtcattccc gcgcaggcgg gaatccagac ctttcagttt ctgtaatgat tgaaaataac
     1801 gtgccgaaat cagccgcacc cggcaggaag ccgccgccac ctgaaacaat gccgtctgaa
     1861 gccttcagac ggcattttat cggaagcgca tcagaaaaaa cggatagcga catatgcggt
     1921 aaaagcaagg gcaattacat agataaaccc ctttataaat tcaaaagtct catctatatc
     1981 gctatatttt tctttcagtt cgtcagattt tcgataacgt ttcattttca agaccacttc
     2041 atgaccaaca agcccgacct tccggattcc cgcctgcgcg ggaatgacgg gcgtgtatat
     2101 ttttgatttc aatctgctgt aaaaatgccg tctgaaatgc aggatttgcg cttcagacgg
     2161 cattgtttgt ttcttattgc gcttcggctc tcggggcgga aatcagaaag tgttcgcggt
     2221 agtggcgcat ttcttcgatg ctctccaaaa tgtcgtccaa tgccttgtgc gaaccgcgtt
     2281 tgaccacgct tttggcaacg ggcggattcc agcgtttggc aagctctttg agcgtggaaa
     2341 cgtcgaggtt gcggtagtgg aagtagtttt ccagtttcgg catatatttg accataaaac
     2401 gccggtcttg gtggatggag ttgccgcaca tcggcgtggc gcgttcgggt acccattccg
     2461 acataaagtc cagcagtttc tgttcgactt cggcttcggt atgcgacgat tcgcgtacgc
     2521 gctgcgtcag ccccgtccta ccgtgtgtgg cggtgttcca ctcgtccata ttgtcgagca
     2581 gctcgtcgct ttggtggatg gcgtaaactt cggattgcgc caatacattc aaatccgagt
     2641 cggtaataat catcgcgact tcgataatgc ggtcggtttc gggattaagc cccgtcattt
     2701 ccatatcgag ccagcatagg ttgtttttat cctgcatgat gtttcctttg cttgtcagcg
     2761 ttttgtcccg cttaatcgcg cccatccttc ttcggtttcc gcaggatcga gatcgaacca
     2821 ttggctgtaa atgccgctga gttcttcgat ttgttcgtcc aacaaaccgg ataacacgat
     2881 gcggccgcct tgtttggtgc gggcggcaag catttcgccg agcatacgta aagggttggc
     2941 gaggatgttg gcaacaacta cgtcgaattg cccttgaggc aggccgtcgg gcaggaagaa
     3001 ttgtgcatcg acgttgttct gttcggcgtt gtccttgccg gcgcggacgg cttgttcgtc
     3061 aatatcgacg ccgacggcag aacctgcgcc gagtttgagg gcagcgatgg tcaggatgcc
     3121 cgaaccgcag ccgtagtcga ggacgctttc gccgtttttg agttgcgtat ccagccattt
     3181 gaggcagagg cgcgtggttg ggtggctgcc agtgccgaag gcgaggccgg gatcgaggcg
     3241 taggttgacg gcagtgcctt cgggggcttc gtgccaagag ggggtaatcc acaggcggtc
     3301 ggaaattcgg atggggtcga attgcgattg cgtgagacgc acccagtctt ggtcttcgat
     3361 ggtttcgccg gtgtatgcca agtcttttaa cccgcattct tgtgcggcga tttggatgat
     3421 ggcggcggct tcgtcgtgtt cgccgaacag ggcgatgact ttgctctgct gccagatttg
     3481 ttcggcgggc ataccgggtt cgccgaaaat cgcctgttcg ttttgcgtgc cggcgtaggc
     3541 atcttcgatg gcggcggaga gtgcgccgtg ttccatcagc gcgtcggcga ggcgttcggc
     3601 gacggcatcg ttgacgttga cggtgatttg ttggtagggc ataacgggct ttcttggaaa
     3661 tatagtggat t
//
LOCUS       DAWHRS010000171         3632 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWHRS010000171 DAWHRS010000000
VERSION     DAWHRS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.171"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..205)
                     /locus_tag="WIB82_001906"
     CDS             complement(<1..205)
                     /locus_tag="WIB82_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ3843157.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYSGLN"
     gene            153..511
                     /locus_tag="WIB82_001907"
                     /pseudo
     CDS             153..511
                     /locus_tag="WIB82_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            542..3394
                     /gene="gcvP"
                     /locus_tag="WIB82_001908"
     CDS             542..3394
                     /gene="gcvP"
                     /locus_tag="WIB82_001908"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ3843158.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAQAEEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGSK
                     EQADQVFAAALESGYNLRRVSDTQVAAAFHETSTREDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPETQTAGYRELLTDMENSLKAITGFDAISLQPNSGAQGE
                     YTGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDTLSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHVVTDMHNASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVERGEWPKEDNPLVNAPHTASDVTGEWTHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
BASE COUNT          858 a         1045 c          930 g          799 t
ORIGIN      
        1 aatttaatcc actataatca tgaaccgact ggaacaccaa actgcttcgg gacgcatatg
       61 ccgtctgaag tgcaaaacct acgccataca gccgcatgaa gttgcagcgg tatggcgttt
      121 tttgaaaaag acggcctgcc ggttcagacg gcatgaccga ccgtccgagc ctgctgtgga
      181 taaagcccgg acaggctgaa atcatggaat attgcgaacc tgaagaagca tccgacccgt
      241 acgcaacata caggcgtgcc aacctgatgg cgaggctgcc gctgtttgtc gtgattttgg
      301 ttctgctcaa tattgttttc cgcttccggc gcatccctta gcttggctgg tgcctgcagg
      361 tttcatggtt ttggtcggcg gctttccctt atcgctgccg cttgtggcgc tgcttgtcct
      421 gacctgctgc attctggcgc gttgtccgcc attatcccgt cttttgtgct acccttgccc
      481 gaatcatccg atgtctaaaa attctgcctg atggcagccc tacaaacccg aaggagtaga
      541 aatgaaactg tccgaactgt tcaaccccga cgaatttgcc gcgcggcatt tgagtttcgg
      601 cgacgaagcg gcgttgcttg ccgctgtcgg cgagaaaagt atggacgatt ttgtcggcaa
      661 caccgtgccg caaagcatcc gtatgccgtc tgaactcgat ttgcccgatg ccctgaccga
      721 agcggacgcg ttggcaaaat tgaaaggcat tgcgtcgaaa aacgtgatca acaagtccta
      781 tatcggttta ggctattacc cgacccgcgt gccgaatgtg attttgcgca acgtattgga
      841 aaatccgggc tggtataccg cctacacgcc gtatcaggcg gaaattgccc agggtcgttt
      901 ggaagctttg ttgaacttcc aacaagtgtg tatcgatttg accggtttcc ctgtggcggg
      961 cgcgtctttg ctggacgaag cgaccgccgc cgccgaagcg atggcgatgg cgcaccgcgt
     1021 gggcaaggta aaatccgagc gtttctttgt ggacgagcgc gtgtatccgc aaactttgga
     1081 cgtgatgaaa acccgcgcca aatatttcgg ctttgaattg gtggtcggtg attttgccca
     1141 agcggaggaa ggcgaatact tcggtgcgct gttccaatac gtcggcaaag acggcgatgt
     1201 gcaagacttg caggacgtta tcggccgtct gaaagccaaa ggcacaatcg ttgccgttgc
     1261 agccgacatt atgagcttgg ttttgctgaa gtctccggct gaactaggtg cggatattgc
     1321 tttgggcaac acgcaacgct tcggcgtgcc tatgggcttt ggtggtccgc acgccgctta
     1381 tttcgcgttt aaagacgagt tcaaacgttc cgccccgggc cgcatcatcg gcgtatccaa
     1441 agacgcatca ggcaaacctg ccttgcgcat ggcgttgtcc acccgcgagc aacacatccg
     1501 ccgcgaaaaa gcgacatcca atatttgtac cgcgcaggca ttgctggcga acttggcggg
     1561 tatgtacgcc gtttaccacg gccctgaagg tgtgaaacgc atcgccaacc gcattcacgc
     1621 gctggcttct gcctttgccg atgcgctggt ttcagacggc ctgaatgtgg ttcataaggt
     1681 cttcttcgat accgttaccg tcgatttcgg cagcaaagag caagcagacc aagtgtttgc
     1741 cgctgctttg gaatcgggct acaacctgcg ccgcgtcagc gatactcaag ttgcggcagc
     1801 cttccatgaa acttcgacac gcgaagattt ggtcgatttg taccgcgcgt ttaccggcaa
     1861 ggatacgttc gcatttgccg atgatgtcaa aggccgtctg aatgccgaat tactgcgtca
     1921 agacgacatt ctgcaacatc ccgtgttcaa ccgttaccac accgaacacg aaatgttgcg
     1981 ctatctgaaa aaactcgaag accgcgactt ggcgatgaac cgcagtatga tttcgctggg
     2041 cagttgcacc atgaaactca atgccactgc ggaaatgttg ccgattactt gggcggagtt
     2101 ctccgacatc cacccctacg cccccgaaac ccaaaccgca ggctaccgcg aactcttaac
     2161 agacatggaa aacagcctga aagccatcac cggctttgac gcgatttcct tgcaacccaa
     2221 ctccggcgca caaggtgaat acaccggtat gctctccatc cgccgttatc aagaagccaa
     2281 cggcgaggga caccgcaaca tctgtttgat tcccaaatcc gcccacggta ccaaccctgc
     2341 gactgcggcc atgctgggct tgaaagtcgt cgttgtcgat accgacgaac acggcaacgt
     2401 caacatcgac gatttgaaag ccaaagccga gcaacaccgc gacactttgt ccgccatcat
     2461 gattacctat ccgtctaccc acggcgtgta cgaagaaggc atccgcgaca tctgccgcat
     2521 catccatgaa aacggcggac aggtttacat ggacggcgcc aacctcaacg cccaaatcgg
     2581 catcatgcag cctgccgaag tcggcgcgga cgtgttgcac atgaacctgc acaaaacctt
     2641 ctgtatccct cacggcggcg gcggtccggg catgggtccg attggcttga aagcccatct
     2701 ggctccgttc gcaccaggcc atgtggtaac cgatatgcac aacgccagtg ccgatcaaac
     2761 tgccgtggct gccgcagctt tcggttctgc gtccatcctg ccgattactt ggatgtacct
     2821 gaccatgatg ggcaaacaag gcatggagca ggcaacacgc tgggcattgc tcaacgccaa
     2881 ctacgttgcc aaacgcttga gcgaagacta tccgattctg tatacaggca aaaacggccg
     2941 cgtcgcgcac gaatgtatcg tcgacttgcg tccgctcaaa gccgaaagcg gcattaccga
     3001 aaccgacatc gccaaacgcc tgatggacta cggcttccac gccccgaccg tctccttccc
     3061 cgttgccggc acgctgatga tcgagccgac cgaaagcgag agcaaagccg aactcgaccg
     3121 cttcatcgcc gccctgaaac aaatcaaaca ggaagtgttg aaagtcgagc gcggcgaatg
     3181 gccgaaagag gacaacccgt tggtgaacgc tccgcatacc gcatccgacg taactggcga
     3241 atggacgcat ccgtattccc gcgaagaagc cgtcttcccg ttgccgttcg tccgcgaaca
     3301 caagttttgg cccttcgtca accgcgtgga cgacgtgtac ggcgaccgca atctcgtgtg
     3361 cagctgccca ccgatggaaa attatgaaga ctgaccgttg aaaccccgga gacaaatgcc
     3421 gtctgaaaca ctttcagacg gcattttcgt cgacggcaga ctggttgcac caatacacgt
     3481 atctcgacta taactttaaa acaaatgagt taaaccagta tccatacatc agctttttta
     3541 tcatcctact ttttattcat ccgatcgtgc aaacagattt caaagatgaa aagcctattc
     3601 acaccctttg atgtcatttc cacacggaca aa
//
LOCUS       DAWHRS010000172         3508 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWHRS010000172 DAWHRS010000000
VERSION     DAWHRS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.172"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            43..708
                     /locus_tag="WIB82_001909"
     CDS             43..708
                     /locus_tag="WIB82_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ3843159.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTANNKCDSGKKEQGMLAFGDKNGNK
                     EYNDTADVLLRSVVLNDDINNKRINYAFNHIAFGHTQPTADRVVWTFNQNGTFGYTKD
                     RHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRNDRRTVC
                     QYK"
     gene            738..1352
                     /gene="pilV"
                     /locus_tag="WIB82_001910"
     CDS             738..1352
                     /gene="pilV"
                     /locus_tag="WIB82_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ3843160.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYIGNHHTLSAVDGDFAVDA
                     VKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGVAPTLSAGSTFSSNCDGSA
                     NGDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            1349..2347
                     /locus_tag="WIB82_001911"
     CDS             1349..2347
                     /locus_tag="WIB82_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ3843161.1"
                     /translation="MRRKMLNVPKGNYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLSAQQDLRNAATLIVRDARMAGGFGCFNMSEHTKNDIIVDPSKQTQH
                     VPVKPGAKQENPLFSLEWANTNNTNNNTAKLIPIAESTDIKYPGFAQARPALIFQYGI
                     DDLDASAETVVVSSCSKIAKPGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYA
                     VGRIADEEGLFRFQLDDKGKWGNPQLLAKKIRHMKVQYIYVSDCPEDDDAGKEEKFKY
                     MGTFDSSTNAVTPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2326..2919
                     /locus_tag="WIB82_001912"
     CDS             2326..2919
                     /locus_tag="WIB82_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ3843162.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNNNEEAFDNIVVQGKPTVEAVKRSCPANSTDLCIDKKGIEYKKGTRSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVGNNDE"
     gene            2924..3397
                     /locus_tag="WIB82_001913"
     CDS             2924..3397
                     /locus_tag="WIB82_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ3843163.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASAQAHLETLSSDVGCEAFSNRKK"
BASE COUNT         1020 a          702 c          875 g          911 t
ORIGIN      
        1 aatttaatcc actatacatt accagttaac gtcctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcatgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacggcca acaataaatg tgactccggc
      301 aagaaggagc agggaatgtt ggctttcggc gacaaaaacg gcaataagga atataatgat
      361 acggcggatg ttcttctccg cagtgtggta ttgaatgatg atatcaataa taagcggatt
      421 aattatgcct tcaaccatat cgctttcggt cacacgcaac cgaccgccga ccgcgttgtt
      481 tggacgttca atcaaaacgg gacgttcggt tatacgaaag accggcatct tacaaaacaa
      541 tccagctttt tttactccga cggttatatc caaatcgtgc tgacagatgc gagagcagtt
      601 tcagatgccg ataagaaatt ccgttcggcg gtggttttga ttgacagcag cggcagggtt
      661 gaggtttgtc ctagaaacga taggcgtacc gtatgccaat ataaatgatt gttttagtcg
      721 gtttgggaaa taaaaaaatg aagaataatg attgcttccg cctgaaaaac ccccagtccg
      781 gtatggcgct gatagaagtc ttggtcgcta tgctcgttct gaccatcggt attttggcac
      841 tattgtctgt tcagttgcgg acagtcgctt ccgtcaggga ggcagagacg caaaccatcg
      901 tcagtcaaat cacgcaaaac ctgatggagg gaatgttgat gaatccgacc attgattcgg
      961 acagcaacaa gaaaaactat aatctttaca taggaaacca tcatacacta tcagctgtgg
     1021 atggcgattt tgcggttgat gccgtgaaaa ctaagacgca gttggcagag gcacaattga
     1081 agagatttag ttatgagctg aaaaatgcct tgccggatgc ggcagccatc cattacgccg
     1141 tctgcaagga ttcgtcgggt gttgcgccga cattgtccgc cggcagtact ttttcttcaa
     1201 attgcgatgg tagtgcaaat ggggatactt tgattaaagt attgtgggta aatgattcgg
     1261 caggggattc ggatatcgcc cgtacgaatc ttgagacgaa cggcaacaat atcgtatata
     1321 cctatcaggc aagggtcgga ggtcgggaat gagacgtaaa atgctaaacg taccaaaagg
     1381 caattatgat ggtatgaagg gttttaccat tattgaattt ttggttgcgg gcatgctcag
     1441 tatgattgtc ctgatggcgg tcggatcgag ttacttcaca tcccggaaat taaatgatgc
     1501 ggcaaacgag cgtctttccg cgcaacagga tttgcggaat gcggcaacat tgattgtccg
     1561 cgatgcaaga atggcagggg gcttcggttg tttcaatatg tccgagcata ctaaaaatga
     1621 tattattgtt gatccaagta agcaaactca acatgtccct gtaaaacccg gtgccaaaca
     1681 agaaaatccc cttttttctt tagagtgggc taatactaat aatactaata ataatacagc
     1741 taaattgatt cctattgctg aatccacaga tattaaatat ccgggttttg cccaggctcg
     1801 tccggcattg attttccaat acggcatcga tgatcttgat gcgagtgctg agactgttgt
     1861 agtcagcagc tgttccaaaa tagcaaaacc gggtaagaaa atatctacct tgcaagaagc
     1921 aaagagtgca ttacagatta ctaatgatga taaacaaaat ggaaatatca cccgtcaaag
     1981 gcatgtggtc aatgcctatg cggtcggcag gattgccgat gaggaaggtt tgttccgctt
     2041 ccaattggat gataagggca agtggggtaa tcctcagttg ctcgcgaaaa agattagaca
     2101 tatgaaagtg cagtatatct atgtttccga ctgtcctgaa gatgacgatg ccggcaaaga
     2161 ggaaaaattc aaatatatgg gtacattcga cagctccaca aatgctgtta cgcccgccgg
     2221 ggtggaggtt ttattgagta gcggtactga taccaagatt gccgcttctt cagacaatca
     2281 tatttatgct taccgtatcg atgcgacaat acgcggggga aatgtatgcg caaacagaac
     2341 actttgacgg gaatcccgac ttctgacgga cagagggggt ttgcactgtt tatcgtgctg
     2401 atggtgatga tcgtcgtggc ttttttggtt gtaactgccg cgcagtctta caataccgag
     2461 cagcggatca gtgccaacga atcagacagg aaattggctt tgtctttggc cgaggcggct
     2521 ttgcgggaag gcgaacttca ggttttggat ttggaatatg atacggacag taaggttaca
     2581 tttagcgaaa actgtggaaa aggtctgtgt accgcagtga atgtgcggac aaataataat
     2641 aatgaagagg cttttgacaa tatcgtggtg caaggcaagc ccaccgttga ggcggtgaag
     2701 cgttcttgcc ctgcaaattc taccgacctg tgcattgaca agaaagggat agaatataag
     2761 aaaggcacga gaagcgtcag caaaatgcca cgttatatta tcgaatattt gggcgtgaag
     2821 aacggagaaa atgtttatcg ggttactgcc aaggcttggg gtaagaatgc caataccgtg
     2881 gtcgtcctgc aatcttatgt aggcaataat gatgagtaat aaaatggaac aaaaagggtt
     2941 tacattgatt gagatgatga tagtcgtcgc gatactcggc atcatcagcg tcattgccat
     3001 accttcttat caaagttata ttgaaaaagg ctatcagtcc cagctttata cggagatggt
     3061 cggtatcaac aatatttcca aacagtttat tttgaaaaat cccctggacg ataatcagac
     3121 catcaagagc aaactggaaa tatttgtctc aggctataag atgaatccga aaattgccga
     3181 aaaatataat gtttcggtgc attttgtcaa taaggaaaaa ccaagggcat acagcttggt
     3241 cggcgttcca aagacgggga cgggttatac tttgtcggta tggatgaaca gcgtgggcga
     3301 cggatacaaa tgccgtgatg ccgcttctgc ccaagcccat ttggagacct tgtcctcaga
     3361 tgtcggctgt gaagccttct ctaatcgtaa aaaatagggc agttttgccg atgccgtctg
     3421 aaaatcaatg ttcagacggc atttttatgg gtatagtgga ttaacaaaaa tcgggacaag
     3481 gcgacgaagc cgaagacagt acaaatag
//
LOCUS       DAWHRS010000173         3465 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWHRS010000173 DAWHRS010000000
VERSION     DAWHRS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.173"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            11..1353
                     /locus_tag="WIB82_001914"
                     /pseudo
     CDS             11..1353
                     /locus_tag="WIB82_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            1398..1688
                     /locus_tag="WIB82_001915"
     CDS             1398..1688
                     /locus_tag="WIB82_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843164.1"
                     /translation="MNIQLQGHIVGVKKINGQIEGKSFDYCCLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1693..1890
                     /locus_tag="WIB82_001916"
     CDS             1693..1890
                     /locus_tag="WIB82_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843165.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            1898..2182
                     /locus_tag="WIB82_001917"
     CDS             1898..2182
                     /locus_tag="WIB82_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843166.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGIFAALFVFSIVKRVMK"
     gene            2189..2467
                     /locus_tag="WIB82_001918"
     CDS             2189..2467
                     /locus_tag="WIB82_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843167.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            2595..2912
                     /locus_tag="WIB82_001919"
     CDS             2595..2912
                     /locus_tag="WIB82_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ3843168.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            2896..3291
                     /locus_tag="WIB82_001920"
     CDS             2896..3291
                     /locus_tag="WIB82_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843169.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
BASE COUNT         1045 a          757 c          811 g          852 t
ORIGIN      
        1 gattcgcacc atggaagata agcaagggat gacaaaggcg gttgccggcg tgatgacgga
       61 cgcgctagcg gacggcagga agccgacaac cgcttcaaat cttcccccct tatctaacag
      121 ggggggacag aaaccgaaac ggcaggcagg gttcaggaag tcttcgaatg ttacgaaacg
      181 tacataacgg acggtaaagg aaacctgtta ggcgttcctc ttcggcgcgg tgtatcagat
      241 tcggctttca ttgatcaaat tagcttttca tttcatgaaa aaaccttttt cgataaatac
      301 ggcgttcgtg taagtctttt ggaagacgaa gattttattc gcgccgcgtc catgctcgcc
      361 gaagaagttt tcggtttcgg tatctacaaa gaatccaaag gctcgggcgg tcgtttctat
      421 gagcgctgtt ggttgatggg ttcggaagac gccctatacg gtcgcgtcca ttttggcggc
      481 caacaaaata ccattctttt cgaactgacc ggcaccggtt gcggcgtcgc aaaagaaggc
      541 tgggaatcac gacttttcgc attcctgact aatgcaatcc gcccaaaaat cacacgcgtt
      601 gacatcgcaa aagacttttt caacggcgaa tacagcccga accaagcccg tgaagaccga
      661 aataaaggta tgtttacctg tcatcacgtc aaaccaaaag gcgaatgttt ggggtcagat
      721 tgggaagaag acgatgaagc caaaatgacc aaaggcaaga cctatggtat cggctcccgt
      781 gaatcgtcca aatatgtccg cgtctatgaa aaaggcaagc agttgggcga taaaacaagc
      841 acatggacgc gatttgaaat tgaattcaaa gcaaaagaca tcgttatccc tttcgaagtt
      901 ttgcagaatc cgggcgaata tttcggcggc gcatatccga tttgcgaacg attcgcccaa
      961 aaggcaacgc gcatacacgc ggttaaggaa gataaggtca tttcagccga ccgctacctt
     1021 gaatgggtaa aaaaacagtt cggacgtgcg gcaaacggtc tgaaattcat ttttcccgaa
     1081 ttggacaaag ccaaactgtt tgaactgatt gagccgagtc atcacaagct gcccaagtct
     1141 ttggctcccg aagcctacga ctgcgccttt ttgaaagctc aagccattca tgaacagccc
     1201 gcattcaaac cgtacaaaga cccttactat atgtacgaat attacgagaa tcttgaaaaa
     1261 cagcttgaac agcaaaaaca cgtcaacaat gaagaaagct ataacaactt catttacgac
     1321 aaattcgcaa gactaccgat ttcatgggct taaagtgtct gcccgaaaga cgtttaatca
     1381 cacaaggaaa ccaaaaaatg aacatccaac ttcaaggcca catcgtcggc gttaaaaaaa
     1441 tcaacggaca aatcgaaggc aagagcttcg actattgctg cctgattgtc gccacaccct
     1501 tagacagctc ccaaggcaac gcattgggca gctctactac tgaatacgat ttcggcggct
     1561 ctgccaattt cgagcagttc cgaaacgccc aatttccgat cgaagcaaac ctgaacgtag
     1621 aaatcgtcac tacgggcaaa acccaaaaac tgaaagtcat cggttttcaa ctcgtgaaga
     1681 aaggctgatt gaatgcagaa agtctatgtt gtccagtccg tatcaacagg ggactttctg
     1741 tatctctctc ctgaaacggg cgacatcgga cataccaaat taatcaccaa tgccgattat
     1801 ttctacgact tcgaagaagc gattaacgca ggtttggaag aaatcggcaa ccaatacgaa
     1861 tttgtcgtat tcggattttt gaaagactga ttttcggatg ttcggcggtc gtctgaaaaa
     1921 cgctccatcc attaccgcca aacacttttt gaaggaaaat atcatgaaat ttattaacac
     1981 ctgccgtaaa tacggcgcaa aactggctgt tgtaacagct gctcccctgg ctttggccgc
     2041 acatgcaaat gcaacgttgc ccgatacggc aaaaaacgct ttggaagccg caaaagcgga
     2101 cggtatggaa gccggttgga ttgtagtggg cattttcgcc gcgctttttg tattttccat
     2161 cgttaagaga gtgatgaagt aagacggcat gtactaccaa gtcggaaata aatgtcttga
     2221 gaagcaccag gctgaaaacc tttatttcag cttggtagta ccaagaatca aagaaaacgg
     2281 acagattgtc aggccggaat ataacggcag cctgtggaag atgtcggacg gtcagccgct
     2341 aaggctttta ttggcggaat gcagtccgaa agacaacctg caaagcggtc ttgaaacagg
     2401 ctggatagta ttcggcatcc tcgcgtccgt ttactttgtt tccctgctga aaaaggtttt
     2461 gaaatgatgg atttttattt ttatctcggc gtttccgtac ccgtattaat cggggcggtt
     2521 ctgtttaaga attgagcgca tgaagttatg gtgtcaaaat caggctttca aaacaacctt
     2581 tgaaaggcag aaccatgaac aagccgttta tcactcaggc gcagttggca ctttataaat
     2641 atcagccgtc aagcaagtat tttgggcaat cgatggcggt tatagcgcaa tctgaatttg
     2701 ttgaatttgc gaagattaat aagtctgaaa atgttattga ttgtttctct tttttctgga
     2761 atagaagaat taaacatgat atttggctaa tctcattttc tgataattca gaaatggtaa
     2821 ttaaagaatc tctgaaagat ggtcataaaa tatacaaatt tgaattttgc gaaattgtcg
     2881 ataattgcaa ttttgatgat gtattcgttt gaagcgaatg caaatgcagt aaaaatatct
     2941 gaaactgttt cagttgatac cggacaaggt gcgaaaattc ataagtttgt acctaaaaat
     3001 agtaaaactt attcatctga tttaataaaa acggtagatt taacacacat ccctacgggc
     3061 gcaaaagccc gaatcaacgc caaaataacc gccagcgtat cccgcgccgg cgtattggcg
     3121 ggggtcggca aacttgcccg cttaggcgcg aaattcagca caagggcggt tccctatgtc
     3181 ggaacagccc ttttagccca cgacgtatac gaaactttca aagaagacat acaggcacga
     3241 ggctaccaat acgaccccga aaccgacaaa tttgcaaagg tctcaggcta agtgcgcctg
     3301 ttgccgccta aaaggtaccc cggatgcctg attatcgggt atccggggag gattaagggg
     3361 gtatttgggt agaattaggg agtgattggt agcggaaata gacgaaaacc tgtgtttggg
     3421 tttcggctgt cgggagggaa aggaattttg caaaggtctc aatta
//
LOCUS       DAWHRS010000174         3462 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWHRS010000174 DAWHRS010000000
VERSION     DAWHRS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.174"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            82..255
                     /locus_tag="WIB82_001921"
     CDS             82..255
                     /locus_tag="WIB82_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843170.1"
                     /translation="MRIRRALKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATK
                     KNGKTVYLPGLHP"
     gene            267..776
                     /locus_tag="WIB82_001922"
     CDS             267..776
                     /locus_tag="WIB82_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843171.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRSRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAISSQRAE"
     gene            complement(829..1176)
                     /locus_tag="WIB82_001923"
     CDS             complement(829..1176)
                     /locus_tag="WIB82_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ3843172.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1178..1414)
                     /locus_tag="WIB82_001924"
     CDS             complement(1178..1414)
                     /locus_tag="WIB82_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ3843173.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
     gene            complement(1773..2264)
                     /locus_tag="WIB82_001925"
     CDS             complement(1773..2264)
                     /locus_tag="WIB82_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ3843174.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(2516..2686)
                     /locus_tag="WIB82_001926"
     CDS             complement(2516..2686)
                     /locus_tag="WIB82_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843175.1"
                     /translation="MPSEIQTAFLLKGFPSTALHKGGFFMPSESLSDGIGLHGRSPPA
                     FKQERMSRMPDF"
     gene            complement(2702..3070)
                     /locus_tag="WIB82_001927"
     CDS             complement(2702..3070)
                     /locus_tag="WIB82_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ3843176.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSQIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
BASE COUNT          866 a          843 c          803 g          950 t
ORIGIN      
        1 gctcatggca atctttcttg caggaaaggc cgtatgctac cgcatactgg cctttttctg
       61 ttagggaaag ttgcacttca aatgcgaatc cgccgtgcgc tgaaattcga ccagttgata
      121 ctcgaatttc ctgagcgcgg ggacggcgca tgggtacaca tcggtttccg acgcaacagc
      181 ccgcaacgca accagatact gaccgcaacc aagaaaaacg gcaaaaccgt gtatctgccc
      241 gggctgcatc cttgaggtcg tctgaaatgg atattttatt gaaatactgg aagccggtag
      301 gtgtattgct gctaatcgtc ctgattttta ccgcatggca tttcgaccgt gccgaaaaat
      361 accgcatggg acgggaggct gctgctgccg aaatctcgaa tcgtctgaaa gacggctata
      421 tcgagcaggc aaagcaggcg cgttctgccg agcagaaggc cgctgccgcg tttgccgaac
      481 gacaaaccaa attagaagag gaaaaacaaa atgctgaaaa aactgttgcc gctatgcgtc
      541 ttgagcttaa ccgcctgcgc cactacgccg ccgcccaaaa tcgcagcaga aacctgcccg
      601 caaccactac cgccgccacc gcatctgatg gcgcgtcaga ttcccaaggc tggctattat
      661 tcggacagtg cgctgaaaaa tatgcaggac tggcagaaac agccgacgga catgcggccg
      721 atttaaggga atggcaggca tatggtcatg caataagcag tcagcgtgcc gaataagcaa
      781 ccgccggaac ctgtaatctt ttcttacagg ttcgggcggt ttcggcattt aatcgaataa
      841 gacggcgccg atgcgcgcca gcacatcgtc caatacataa tcgggtacag tttctttaaa
      901 agaagccttg cggatttgcc agtccagaga tttgagctgg tggcagtgga cattgccctg
      961 cgtttccgtt cctgcaccga gtaaggttga aatcatgccg ctgcttcgtg cagccgctgc
     1021 attcccctgt gaaatggggc aggcaaaaac caatcccgtt gcgcggttga atgcttttgg
     1081 agacagagcc agcgcaaacc gcccgccctt gatttccttg ccgctggaag ggtcgaaatt
     1141 caaatggaaa atatcgcctt tgtcgggaat atacatttca gacgacctcg ttgccgacat
     1201 catccaagat ttcccagcct tctacgcgcg gcggggtttc ttccatttcg gcaagcaagt
     1261 ctgccaagcg gaaacgtcgg gcagcacgca cacggagttc gccgttatgt acttccgcta
     1321 ccaaagcgtc gccgatttta aaatccaatt gtttcagcat gtcggcaggc agtcggacgg
     1381 ccgccgagtt cccccatttt tggacacgca acataatctt cacctttatt gtatctacaa
     1441 agtagataca tattaccata aaatttcagt tgttcaaata cttgtgcaga atacgccaaa
     1501 agccgtccga actgtttcgg acggcttttg tactgtattt gcgccttcag gcaatatttt
     1561 gttatccatt ttcaagatgc aaaagctttc taattgcttg atgtcggatt tcggttgttt
     1621 agggatacaa aaccaagtaa actaaaactg tttgattgga aaatgctccg caaggagaaa
     1681 attatgttca aaaaatcact ttataaggct gctttggcgt atttcggcga ttgcgtggct
     1741 gcccatattt cagaacagtt ttgactgttt attcacaaat cagatgcctt tagggggttt
     1801 gctttccata atgcaaccaa aatttccaac tctctaaata ttgtgtcttt gcgttcttct
     1861 tcgcgtattt tcataacaag aggcgaaact gcattccaca attttatgaa gttggtgcaa
     1921 tgcagccgtt taaacaaatc ctcatccaat actcccgagt tgattgcgca cgcataaaac
     1981 tcgtgccgat tgattacggt aagtaaatgc cctctctctt tttcccattg tccgccctta
     2041 ttttgataaa tttcatacag gtcgatccgc gcgttgttgt cgtcaactgc cgtgccgcga
     2101 atataaggcg aaatatgatt gtcggcttct acaaattgtg catcttggta atcattcaaa
     2161 ataacatcta atgtcgcctt ttgctttgaa gttttcttat tgatgaatat tgtccccagt
     2221 gcaatgacgg cggttactga aacaacggtc agttgccaaa acatcagcca ttcggccgtc
     2281 cctcataggt taaactgtac gctgtgcctg aatatgtaaa ataaagtgaa tacagataca
     2341 aacaaggagg ttagggcaat tactgacaac caacctcctt gttttatttg tttccgcacc
     2401 ttaccagcca ttaaaaccct catttttgta tttcatcgta tttccttaat ggttttaacc
     2461 ttgcttgtcg atatctgtat attaatgccg tgcgcccgtt ctttcaaggg gtgatttaaa
     2521 aatcaggcat ccttgacatc ctctcctgct taaaggcggg gggactcctg ccgtgtaaac
     2581 caatgccgtc tgaaaggctt tcagacggca taaaaaaacc gcctttgtgt aaagcggttg
     2641 aaggaaagcc tttcaataaa aatgccgtct gaatttcaga cggcattgtt gtcggatatg
     2701 cctattcctt atccagccgg cgcagggttt cggcgagctg tttgaagtcg gtttctcccg
     2761 ccgccaaact gacaatcaag tcgtcaagcc cttggtcggc ggcaatgctg atgccctgca
     2821 aatccagata ggtcaacatt gttaaaagcg cggtgcgctt gttgccgtcg ggaaaggcgt
     2881 gggctttggc tatggcttgt gcatagaggg cggcgatttc gtagatgtcc tcaaggtttt
     2941 catactgccg ccagttggca atctgcgaca atgcgccgtc caagcgcgcc atatccgccc
     3001 gccctttcaa acccgcttca tccgccaata cggtttgatg gataagcgcg accagttcgc
     3061 cgtctatcat ttgtcggcaa gtgccttgac ggctttttga tgggttttgg caatgcggcg
     3121 ggcggcggca agcagtacgc gtttgcctgc ttcgccttta agctcgattt tgacggggcg
     3181 tgtattttgg ttttgcatcg ctgctccttg tctgtttgcg ggcattttag cttttttccg
     3241 gcagcttggg aaatgccgtc cgaaaacact tcagacggca ttcttctaat agtgtaatgc
     3301 aatagttact ccaagatttt tgtatatggc tgtttttatg gtaaattaaa ttttcataag
     3361 aaataaatat atttaaatca atgaaataaa atttagtcga atcccaccgt ttccgccaca
     3421 aaacaaaacc gccctgattc ggggcggttc ttttttgttc aa
//
LOCUS       DAWHRS010000175         3422 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWHRS010000175 DAWHRS010000000
VERSION     DAWHRS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.175"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(69..302)
                     /locus_tag="WIB82_001928"
     CDS             complement(69..302)
                     /locus_tag="WIB82_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843177.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFLSFSHFR"
     gene            complement(332..3097)
                     /locus_tag="WIB82_001929"
     CDS             complement(332..3097)
                     /locus_tag="WIB82_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3843178.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHYSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQDGQLKFNSDSGKWER
                     DLQRQQWKYPWYKKYENPQELQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTRDVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
BASE COUNT          788 a          834 c          852 g          948 t
ORIGIN      
        1 ccgtacggaa acctgcacca cgtcattctc acgaaagtgg gaatccggaa agctaaagcc
       61 acaggaattt atcggaaatg gctgaaactt aaaaaaactg attcccgctt ttgcgggaat
      121 gacgactcat aaattacccg aaacaaccaa aaataaccga aatcaaaaac cggcttccct
      181 gcgtgggaat gaaatggatt tttctttgag ttgccgatgc gataaaacgg attggcatag
      241 gcttgcggca ggatgatgcg tttggggaaa caaatgccgt ctgaagggct ttcagacggc
      301 attgcggttt tccacaaaac gcgggctgcc tttaaaactt gtagctcatc gttatcaaaa
      361 aggtgcgtcc gcgtgcaaaa ttggtcaata cgcttttgct tgtgccgccg tatttgccgt
      421 tgcacaagcc atcagcatta cacgttacgt cttcgtcctt gtctttcggg tcgaacgagc
      481 tgtaataacg ctgcgttgcc gcatcattgc ccgcatcgag cggatcgata taacgcctgt
      541 cgaacagatt tttgacttcg gcgcggaaaa taaggttttt cttcggctcg taagcggcgt
      601 aaaaatcaaa aatcaaaggc tggcgggcaa gggtttcggt ttgtttgatg gaacgcttgc
      661 ccagttgccg gacatcgcgg gtatttcccc cgttggtgcc gtcgatatag cgttcttcag
      721 ccgtcgcgcg gatgctcttg ccgaaatagc gcatcgcgcc gcccaaagtc agcttgttgc
      781 ccaaccagcg cgtaccgact tccaaacgtc cgtaatctcg cggcagggcg gaaaccctgc
      841 tcaacccata accttgtttg agttggtctt ctttggacgc attgttgggc gattcgctcg
      901 catcgctgaa gttggtcggt tgcgtgcttt tttgataggc gtaagaaagg ttggtgaaaa
      961 aacgcccata atcgtaattc agctccaact caaaaccgtg tttgtgcact ttgtctttga
     1021 aattgcgatg ttggatggtg taggcaagcc cggtgctgct gacccagctc ggaatatccc
     1081 cgttcaaatc ccaccatttc ccgtaaacgt tgtggatgta gttgtcgatg cggctgcggt
     1141 agccgaccag ttttaatcct aatgtatcat cttgttttaa cagtcctttt ttataggtat
     1201 tgaagccaaa ttgccaagtg tttgcgcgct ctggttttaa ggcggtgtga acgccggagt
     1261 cgccgatttg ggaaaaatac atttcttgga tgttgggcat acggtgtgtg cgcgaatagc
     1321 tggcgaacgg catgaaataa tcgccgaagt ccgcactaat gctgaccgaa tggttgttgg
     1381 cgcgcttttt gccgtatttt ttcaatacgg gttcataaag cccgcagctc gggttgcaat
     1441 gtttcttgta tgtcggcgag ttttctccga atgcccgctt aaattcgcct tccgagctgt
     1501 aatagcccgt atattcgccg ccgaaacggt agccgacggt attggtgctg tagtttaagc
     1561 ggtaaatgtc ttttttgagc gcggcatcga agtagaacgt gttgaaatat tggctgccgg
     1621 ccggttggac aatggttgat ttttggggca gcagcccttt atcgccctta aaccgcccca
     1681 aataggaata aagcccgttg tcctgatcag gaccgtcgaa aaacagcccc aattcttcag
     1741 gaaagcggtt tttgccgtat tcgttgtgga aataattgaa gcccaaagtg gtttgcaact
     1801 cggtttcgcg gggcagccgg aaggtggcgg tgttgttgag gtcgaggatt tttgcgttgt
     1861 tgtaggtttc aaaatccttt aaaagccccc agcctgtaaa cttcgaccct ttcggatatt
     1921 tctgcctgcc cgaattgtag gctgcggtca gattgaggtt ggtatacgag ttcaaagaca
     1981 aaccgtaatt gaattgataa ttgcggttga tgattttgcg gctgccgatt ttggtgttta
     2041 aatcgcgaaa ttgcgccgtg tatttattga atacgccgtc gtattccaat ttaaacagat
     2101 tacctgccga ctgctgcttc aggctggacg gatcgatggg ggtgatgtcg tattgcggcg
     2161 ccaggttttc ccgccagctt ttgtcatgct cttcgatgta tttttgcagt tcttgggggt
     2221 tttcgtattt tttataccac gggtatttcc actgttgtct ttgcaagtcc cgctcccatt
     2281 ttccgctgtc ggaattgaat ttcaactggc cgtcttgtac aaaatatcgt tgcttgcgcc
     2341 gttccagata ttccgcgcca aaatttccga tgtgctgccc gccgccgccc acgcggtaat
     2401 tttgcgccac gctgcgcctg ctgtgcccgt aaagcacacc gacagatgct ccgctttcca
     2461 gccatttgcg cgcacctatc gccgccatcg cattaccttt ggttgaattg gtgccggtca
     2521 gaccttttag cagcaggccg taggtattat tgccctgaac gacgtcatcc acgcctaaag
     2581 tccgcagatt cgccgaaccg gcaaggctgt tgatgcctgc cgagccgctg aagctgcctt
     2641 tgacgacatc cagtccggca ataaaattgc tgtcgacaga tgcaccgaat tgagatgaac
     2701 cgcctgccct gcccgcatcg gtagaagtcg aataaaaggt ctgcgtgatg ccgtccacca
     2761 tcgtattgac ccgcccgaac ccgctgtcgc cgcgaatatt caaagacaca atgcccgagc
     2821 ttttatcttg ctgtgtaaac gcaccgggga tgctgcgtac gatgttgtcg aggttttcgc
     2881 tggatttgaa tatatcctga cgggtcgata cggcacgcgc atcggtaaac acttttttgt
     2941 ctttcggtac gcgcttcgcc ttgacgtgca catcttccaa aacctgtatc tgcgcctcgc
     3001 tgcccgcgcg ccctgcatct tcggcataac tataatgata tagcataaca cccataaggt
     3061 aaaaacaaat cggcttcaac cggaaagaag atctcatatt ttcctcaaca ataaacagtc
     3121 agacaattag gaaatatact agcatttttt tcaggcggca taaacattaa aaaagtgtaa
     3181 atttgatata ccgtctgaag atttcaattg gatatttata gtggattaac aaaaaccagt
     3241 acagcgttgt ctcgctttag ctcaaagaga acgattctct aaggtgctga agcaccaagt
     3301 gaatcggttc cgtactgttt gtactgtctg tggctcgccg ccttgtcctg atttttatta
     3361 atccgctata aagaccgtcg ggcatctgca gccgtcattc ccgcgcaggc gggaatctag
     3421 tc
//
LOCUS       DAWHRS010000176         3421 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWHRS010000176 DAWHRS010000000
VERSION     DAWHRS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.176"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(156..2288)
                     /locus_tag="WIB82_001930"
     CDS             complement(156..2288)
                     /locus_tag="WIB82_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ3843179.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(3070..>3421)
                     /locus_tag="WIB82_001931"
     CDS             complement(3070..>3421)
                     /locus_tag="WIB82_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3843180.1"
                     /translation="VYTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLHVSGF
                     IHHRINHSKEFADRQNHINGIENFWNQAKRALRKYNEIDRKSFPLFLKECKFRFNFGT
                     PSRQLKILRDWCGI"
BASE COUNT          979 a          917 c          761 g          764 t
ORIGIN      
        1 gagtgaaaaa gaagtgcagg ctgcctaaaa agacagaaaa agtctttccg gcagcctgca
       61 ctttggtttc atttcagtca gtaaacccag taaacgacgg tctaaaaacg cagaacgtta
      121 cgaaaaaagc agcctacacg cccatccccc gccttctacc cgttctgtaa atcatacaga
      181 tagcggtaat atccgttcgg cttcgccagc aattcctgct gtgttcccgc ttccacaatc
      241 ctgcctttat ccatggcaat gatccggtgt gccgttttaa cggtggacag acggtgggcg
      301 ataatcagca ccgtccggtt ggcgcaaatg gcctgcatgt tctgcataat cgctcgttca
      361 ctttcataat ccagcgcgct ggtggcttcg tcaaaaatca gaatgcgcgg attggtgatt
      421 aacgcgcggg caatcgcaat acgctgccgc tgtccgcccg acaagccggc cccttgttcg
      481 cccaccacgg tgccgtagcc ttccggcagc tccataataa actcgtgtgc gcccgccagt
      541 ttggctgctt cgataatgcg ttccagcggc atacccgtat ccgtcagcgc gatattgtcg
      601 cgtatgctgc ggttgagcag cacattctcc tgcaagacca cgccgacctg ccgccgcagc
      661 caggcaggag cggccaaagc caaatcgttg ccgtccacca acacccgtcc ctgctccggt
      721 acatacagac gctgcaccaa tttggtgagt gtggatttgc ccgaccccga acgtcccaca
      781 atccccagca cttcccccgc ccgaatccgc aggttcaaat cctgcaaaat cagcctgccg
      841 tccgccttat agcggaaatc gacatgttcg aacgtaatct ccccccggat atcgggcaaa
      901 gccaaatgcg aagacgcatt ctcggtcggc gcattcagaa tatcccccaa acgcgccacc
      961 gaaatcccca cctgctggaa atcctgccac aactgcgcca aacggataac aggcgccgcc
     1021 acctgtcccg agagcatatt aaacgcaatc agctgcccca ccgtcagctt gccctcaatt
     1081 accagccgtg cgccaatcca caacgtcgcc accgtcacca gcttctgaat cagctgcacc
     1141 ccctgctggc cgaccaccgc caacttcgtt acccgaaatc ccgaagccac ataagccgcc
     1201 aactgattgt cccaacgctg cgtcatctgc ggctccaccg ccatcgcctt taccgtaccc
     1261 accgcagtga tgctttctac taaaaacgac tggttgtctg cattgcgcgc gaacttatcg
     1321 ttcagacgcg tccgcagtat cggactgata aatgccgacc aaaacgcata ggcaggcaac
     1381 gaagccaata ccacccaagt cagagtggag ctgtaatacc acatcaccgc cagaaagata
     1441 aacgaaaacg ccaaatccaa caccgaagtc agcgcctgac cggtcaagaa attgcgaatc
     1501 tgctccaatt cccgcacccg agccaccgta tcacccactc gtctgtgctc gaaataggat
     1561 aaaggcaggg aaagcagatg ccggaacaaa cgcgcgccca attccacatc aatacgtgaa
     1621 gtcgtatgtg caaacagata cgtccgcaaa ccgcccaaca caatctcaaa cagcgacacc
     1681 accaacaaag ccaccgacac cacatccaaa gtagagaatc cccgatgtac cagcaccttg
     1741 tccatcacca cttggaaaaa cagaggcgta atcagcgcaa acagctgcaa caccaccgac
     1801 accaccaata cttcaaaaaa caaccggcgg tatttgatta ccgccggaat aaaccaggta
     1861 aagtcaaact ttgccaaact gcccaatacc gaagcgcggg aagcaaccaa tatcagtttg
     1921 cccgaatatc tgttagaaaa ttcggcaaaa gacaataccg cagacttatt cgtaaccaaa
     1981 tcctgtatca aaaattgggc atgctcaccc tcaccgtctg ttttggccaa aatgaaatgg
     2041 ttgccgtcat cacaccatac caatgcgggt aaagtcgcca tagccaaacg tttaataggc
     2101 tggcggacta cctttgcctt caatcccaaa gatttggcgg ctaacagcca ttgcgtttca
     2161 tttaaatcgc tctgtgcgga agtacaaaat tcatgctgta tatcggcagg attggcggca
     2221 atgccgtggt aatgggcgag gatgatgagg gcggaaaggg cggggagcgg tgcggatacg
     2281 atagacataa ataaaatata gttagattgg atgtggataa cggctggctg gaaaaggaat
     2341 atattaagta gaaagaaata tataaataaa acagcagaac gcattgtaag gatatatatg
     2401 ggaattgtaa agagaaagta tggaaaagtt ctcgtttcag gaaggtaaaa cggcttagga
     2461 atcgagttag atgaggatgc ctcgcacctc tcgtgcctcc tgcataccgt taaggaacag
     2521 ggttaaggtg caggctgctc cgaactctgt tgcggtcgga taatgttatt ttttgtgttt
     2581 cagtcagcct gaaatatctg tatatttttg ttttaaatag attttaaaga ttgataactg
     2641 ttcttgacga tttttcaaga aaggagtaaa gtgacttatt atcaatgaca agcaacgcgc
     2701 gaagtgacaa ggaaaactat ctacttaaat tctaaggagg cttcgaatat cataaaccaa
     2761 tcagaaacat agagataaaa attatgtaca aatataatcc tcttatacaa tttattgcac
     2821 agttgattat gtcttatgga gcaagcatag ggtgggcact tgctgcccac gcgtttcata
     2881 tttcaaggca gcctgaaact gtgtgggcat aatgcctacc ctacatccca aaaaaacaag
     2941 cgcagcctgc gtgtgtaggg tgcgaacttt cggcaggcag acacgcggtt ttatagtttc
     3001 aagctgagtt gggatgctta agataaagta caaaacctta aaaaataagg gggctgtact
     3061 agattagccc taaattccac accaatcccg caaaattttt agctgtcggg acggtgtgcc
     3121 gaagttaaac cgaaatttgc attctttcaa gaacagcggg aaagatttac gatcaatttc
     3181 gttgtatttg cgcaaggcgc gtttcgcctg attccaaaag ttttcaatgc cgttaatgtg
     3241 gttctgacgg tctgcaaact ccttagaatg gttgatgcgg tgatggataa aaccgcttac
     3301 gtgcagcttg tcatagctgc tcagactatc ggtataaaca atactgtccg gcatgatttt
     3361 cttcttgatg acagggagta acgtttcaga cttggcatta tccaccacaa cggtatagac
     3421 c
//
LOCUS       DAWHRS010000177         3400 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWHRS010000177 DAWHRS010000000
VERSION     DAWHRS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.177"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(5..823)
                     /gene="cysE"
                     /locus_tag="WIB82_001932"
     CDS             complement(5..823)
                     /gene="cysE"
                     /locus_tag="WIB82_001932"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ3843181.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            1007..1585
                     /gene="grpE"
                     /locus_tag="WIB82_001933"
     CDS             1007..1585
                     /gene="grpE"
                     /locus_tag="WIB82_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ3843182.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(1709..1924)
                     /gene="nqrM"
                     /locus_tag="WIB82_001934"
     CDS             complement(1709..1924)
                     /gene="nqrM"
                     /locus_tag="WIB82_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ3843183.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(1950..3005)
                     /locus_tag="WIB82_001935"
     CDS             complement(1950..3005)
                     /locus_tag="WIB82_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ3843184.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDSASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(3299..>3400)
                     /locus_tag="WIB82_001936"
     CDS             complement(3299..>3400)
                     /locus_tag="WIB82_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3843185.1"
                     /translation="YFGLSKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          760 a          796 c          986 g          858 t
ORIGIN      
        1 atattcaaat cataaagtcg atttcggtaa actggatatt ttgatccata tccgccgacg
       61 gtgttttgag cgatcgcgcc acgggtttgg cgggtacgcc gacaaccgtg atggacggcg
      121 gcacgtctga aaccacgacg ctgcccgccc cgattttggc attgctgccg atgcggatat
      181 tgcccaatat cgaggcgttt gcgccgatca tcacgccgtc gccgatttta gggtggcggt
      241 cgccgccttc tttgcccgaa ccgccgagcg ttacgccgtg caaaatcgaa atattgttgc
      301 ccaacacggc ggtttcgccg gcaacaaagc cggtggcgtg gtcgagcatc agcccgtatc
      361 cgaaacgggc ggcgggatgg atgtccacgc cgaatacttc ggacatacgg ttttgcagga
      421 aatacgccag cgttttgcgc ccgtcgagat acagccggtg gttgatgcgg tgtgcctgaa
      481 tcgcgtggaa gcctttgaaa tataaaagcg gcagcgaata ttcgtcgcag gcgggatcgc
      541 gttcgtagat ggcttttaag tctgcttcga cgcatttgcc gatttgggtg tcgctgccca
      601 acgcctgctg gtagatttca aacagcgcgc gcacgtccat aatcgggctg ccgagtttgc
      661 tggaaaggtg gtaggcaagg acggagccga gggactcgtg gcgcaacacg gtttggtgca
      721 aaaaacttgc cagcatcggt tcggcggaga ccgcggccgc ggtttcttcg cggatggtgt
      781 gccagaggtc gaaaccggtt gtgtttaaat ggtctttttt catgagtgat gacgtttgaa
      841 aatcgatatg gtcggcagta tcttaccgtc tatattattt tttcggtagg ggatttgaaa
      901 atgaatttga aattctctgc ttttgcttga agtttcttga aaatgtcctt atcttgcgcg
      961 ggtaataact ggattttgat ttccaatttg ttttaaggga tacgatatga gcgaacagac
     1021 acagcagcaa aacagtgaag aagcggttga aaatgtggag gcggtggaaa ccgtcgagac
     1081 agtaggaaat gcggacggtg tgcaggaaca ggctgccgca gagccggctt atgaggattt
     1141 gcaggcgcgg attgccgagc tggaagcgca gttgaaagac gagcagctgc gcgctttggc
     1201 aaacgagcaa aacctgcgcc gccgccacca gcaggaaatt gcggatacgc acaagttcgc
     1261 cggacagaag tttgccgtgg aaatgctgcc ggtcaaggat tacctggaaa tggcgctttt
     1321 ggatcagagc ggcaatttcg atgcgctgaa aatgggcgtg cagatgactt tgaacgagtt
     1381 gcagaaagca tttgatgcta cgcaaatcaa ggaaatcaat cctaaagcgg gcgataagct
     1441 cgatccgaat atccatcagg cgatgcaggc ggtggcaagc gaacaggaac ccaataccgt
     1501 ggtgggcgtg atgaagaagg gttatacgct gtccgaccgc gtgttgcgcc cggctatggt
     1561 tacggtggca cagaaggaag cctgaaggcg tctggggaat aatctgattt atttcctgaa
     1621 gcgcgttttg cgtataaacc gatcgaagta aagcggcaat gccgtctgaa cccgcctgtc
     1681 gggcttcaga cggcattttg tttcggcgtt aacggtcaac ccgtatgccg cctgcctgtt
     1741 tttcttcatc cagtttcttt tgcagggtgt cgcaaggtgt gtcgcagtcg cacatttttt
     1801 tcatacccaa ggcagtaatg ccgccgcaac tgcctttgat gctgcgtttg gagaaaatat
     1861 agccgaccgc cataccgatg atgacggtca ggaagatgcc gaaggtaagg agcagggttt
     1921 tcatggtgtt tcctaatcgg tttgtatgtt tagcggagca gtttttcaaa ttcggaagac
     1981 atcgcggtgc ggtagccgcc tttatccctg acaatcagga aaacagcgag tttttcgcgc
     2041 tctgccagct ttaaggcttc ggtttcgccc aatacgaata atcctgtgga caagccgtcc
     2101 gccgtcatcg cactgtctgc gaccacgctg atggaggcga ggttatggct gatgggtcgt
     2161 ttgttgttcg gattaatgat atgggagagg cgtttgccgc ttttatcgac gtggaaaata
     2221 cggtaatcgc cggaagtggc aagcgaacgg ttgttcagcg ggacgataat ctgcgtattg
     2281 ccgccttgga tgatattggg ttgctcgata ccgatgcgcc acggttcgcc gcgcgcgttt
     2341 ttgcctttgc cgtgcaactc gccgccgatt tcgaccagat aattttgaat gccgtatttt
     2401 tccagttcgc ccgcaacttt atcaacgccg aagcctttgg caatcgaaga taaatccaaa
     2461 taggctttgg ggtgggtttt gctcaaggag gcggaatctt tgccttgttt caaaatgatt
     2521 ttgtctatgc ccgtataaga tgccgcctgt ttgatttgtt ccggcgacgg ttcacgggta
     2581 acggatttgt cgggcccgaa tccccaaagg ttgaccaaag ggccgacggt tacgtccagc
     2641 gcgccgtgtg tcaggcggtt caggcggaca gcttcggcag taacgtgtgc gaagtcgctt
     2701 gaaatgcgga ggggtttgcc ggccgtggtt tggttgaacc ggctgatttc ggaattaggc
     2761 tgataggtgg acatctgccg gttgacttct ttaagcgcgt catcgatgcg cttttgtatt
     2821 tcggcaggtg aggggagttt gtcccgatta tttgaaaggt atttgacggt ataggtcgtg
     2881 cccatcgttt cgccttgcag ggtaacggtt tgcgcggttt gttccgaaca ggcgttcagg
     2941 aagatgaaac ccagggcaaa tatcaagacg cggataaagt tcggcaggcg tgtttcagac
     3001 ggcatagtgt ttgacggttt tggcaaatgg tttgaattat atcgcaaaac ggccgatatg
     3061 tttctatgcc gatgccgtct gaagggcatc aggggcatcg cccgctgttt gggggcggta
     3121 ataaaaaaac aaaccggtgt tttacaaacc gagacctttg caaaattcct ttccctcccg
     3181 acagccgaaa cccaaacaca ggttttcggc tgttttcgcc ccaaataccg cctaatttta
     3241 cccaaatacc cccttaatcc tccccggata cccgataatc aggcatccgg gctgcttttt
     3301 aggcggcagc gggcgcactt agcctgttgg ccgctttcaa aaggttcaaa cacatcgcct
     3361 tcagatggct ttgtgcactc actttgctca gcccgaaata
//
LOCUS       DAWHRS010000178         3311 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWHRS010000178 DAWHRS010000000
VERSION     DAWHRS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.178"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..331
                     /locus_tag="WIB82_001937"
     CDS             <1..331
                     /locus_tag="WIB82_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3843186.1"
                     /translation="LPKGTTVYADKGYDSAENRQHLKEHQLQDGIMRKACRNRPLTET
                     QTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANR
                     LSAPAAA"
     gene            complement(799..1752)
                     /locus_tag="WIB82_001938"
     CDS             complement(799..1752)
                     /locus_tag="WIB82_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843187.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            2136..3155
                     /gene="galE"
                     /locus_tag="WIB82_001939"
     CDS             2136..3155
                     /gene="galE"
                     /locus_tag="WIB82_001939"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ3843188.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMQPGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNNP
                     NGYDD"
BASE COUNT          968 a          668 c          686 g          989 t
ORIGIN      
        1 gttacccaaa ggtacgaccg tctatgccga caaaggctat gacagtgcgg aaaaccggca
       61 acatctgaaa gaacatcagt tgcaggacgg cattatgcgc aaagcctgcc gcaaccgtcc
      121 gctgacggaa acgcaaacca aacgcaaccg gtatttgtcg aagacccgtt atgtggtcga
      181 acaaagcttc ggtacgctgc accgtaaatt ccgctatgcg cgggcagcct atttcgggct
      241 gattaaagtg agtgcgcaaa gccatctgaa ggcgatgtgt ttgaacctgt tgaaagccgc
      301 caacaggcta agtgcgcccg ctgccgccta aaaggtgccc cggatgcctg attatcgggt
      361 atccggggag gattaagggg gtatttgggt aaaattagga ggtatttggg gcgaaaacag
      421 ctgaaaacct gtgtttgggt ttcggctgtc gggagggaaa ggaattttgc aaaggtctca
      481 ggctgcattt ctcaatgtaa aacgcgatat tttcacccat tattagggat aagtagccag
      541 tggtttacac tggctacttt catagttatg aagtactggg tctgagtgaa ttcaattatt
      601 tccgtataaa acacagtaga cattttacta atcggcaaaa ttatattaga agaattgaga
      661 actactgaag tcaggtaaga taccattttg cacaaattta acggcatccc taaaaagcat
      721 tttgggtggt atttaaagga gtacaaaatg gttttaacta cagtatgctt agaaatctat
      781 agccttacgc gctttatatt aaatcacctt tctcaaccat ttagaatcat aaaaaaactt
      841 tttaggatcc ctaattaact ttttaaattt tcttaaaaaa atagttttag ttgattctat
      901 actattcacc gcagataaat attcttggta atcatgttgc aatggcacaa cctgctttaa
      961 tagcttatta aactcatcag ttgtaatcac ctttttagta gcatagcaat caagccaatc
     1021 cggatctgct atttttgggg aagtgaaatc tatttgatca aaataaacta aaaccccact
     1081 acctaaaaaa tgctgatttg cataggattt agaaaagcct aagtctgcaa caacaccaac
     1141 agggataccc ttacattcag cttcaaaaag catagtagat gaataagata aacaataccc
     1201 catttcttgg aaggcttgcg cggtactttt tcgctctatt gtcaaattac tcggcaactg
     1261 aaactgcttt gccagctcta tatacgaatg tttatcctga tgcacagtaa tatctttatc
     1321 tgcaaccctt agcaaaatag taaattcttt ctcagggtat ttttcagcca aggcaatcaa
     1381 tttttttaat gtataaattc tttcttcttt tttaaatggg attttaactt ggtcaataaa
     1441 gtaaattttc tctccatgac gaccttttga tgcgtggcga aaaattggat aaccataaag
     1501 tatattttga caacttaatt tatattgttt cttatattcc tctgcaattc taaaatcatg
     1561 cttattatta tataaaacaa tatcagcccc catacgagat agaatacttg cctgatcacc
     1621 gaatactaca cctggaaaca aggtaataat caatggcctt gaagcaatat ttaattgcgc
     1681 attctgctta aagaaccttt ttaacaaccc atttccaacc gataaaatta ctgcgtcata
     1741 atattgatgc atattcttaa taaagtaatc atcaatatga aaaaaaaaaa ttgcttggtc
     1801 tttatctatc cctgattcta ataactgtcg atttgaaaga atgttttctc gacttttgtg
     1861 gatataaata tcaatttgag catcttttat ctcttttgca acagcatagc ccgagttaag
     1921 gaacgagtca taactggcaa ttagtaatac ttttttcact attttccctt aattctacac
     1981 catatgaata aaattatagt attttacctt agatatcaaa tatacttaac caaaaaaatc
     2041 tgaagaaatc tactgctact tcagggataa ttatttcatt aagattttac aaccattatt
     2101 atcaatctat caaagtattt ttagtatttt attctatgaa aaaaattctc gttaccggcg
     2161 gcaccggttt tatcggctcg cataccgttg tttctttgct gaaaagcggc catcaagtcg
     2221 tgattttgga taacctatgc aattccagca tcaatatcct gccacgcttg aaaacgatta
     2281 ccggccaaga gattccgttt tatcaaggcg atatccgcga tcgtgagatt ttgcgccgta
     2341 tttttgcgga aaaccgcatt gattcggtga ttcattttgc cggcttgaaa gcggtgggtg
     2401 aaagtgtggc cgagccgatg aaatattatg ataataatgt ttccggcagc ttggtgttgg
     2461 cggaagaaat ggcgcgtgcg ggcgtgttta gcattgtgtt cagttcttcg gcgacggttt
     2521 atggcgatcc gggcaaagtg ccttatacgg aagatatgca accgggggat acgaccagcc
     2581 cttacggcac atctaagtct atggtggagc gcattttgac ggatatccaa aaggccgatc
     2641 cgcgttggag cgtgattttg ttgcgctatt tcaacccgat tggggcgcac gaaagtggtt
     2701 tgattggcga gcagccaaac ggcatcccga ataatttgtt gccttatatc tgccaagtgg
     2761 cggcaggcaa actgccgcaa ttggcggtat ttggcgatga ctaccctacc cccgacggca
     2821 cggggatgcg tgactatatt catgtgatgg atttggcaga aggccatgtc gcggctatgc
     2881 aggcaaaaag taatgtagca ggcacgcatt tgctgaactt aggctccggc cgcgcttctt
     2941 cggtgttgga aatcatccgc gcatttgaag cagcttcggg tttgacgatt ccgtatgaag
     3001 tcaaaccgcg ccgtgccggt gatttggcgt gcttctatgc cgacccttcc tatacaaagg
     3061 cgcaaatcgg ctggcaaacc cagcgtgatt taacccaaat gatggaagac tcatggcgct
     3121 gggtgagtaa taatccgaat ggctacgacg attaagttga cctgatacag gccgtctgaa
     3181 agagatgttt tcagacggcc tctttatctg aaaaacacac attctgtctg ctataatctg
     3241 tttatatttt ttggctatcc tctgaaattt atgagaaaaa tccttgttac cggcggtgcg
     3301 ggctttatcg g
//
LOCUS       DAWHRS010000179         3296 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWHRS010000179 DAWHRS010000000
VERSION     DAWHRS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.179"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(75..668)
                     /gene="ribA"
                     /locus_tag="WIB82_001940"
     CDS             complement(75..668)
                     /gene="ribA"
                     /locus_tag="WIB82_001940"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ3843189.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(661..1335)
                     /locus_tag="WIB82_001941"
     CDS             complement(661..1335)
                     /locus_tag="WIB82_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843190.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1489..1573
                     /locus_tag="WIB82_001942"
     tRNA            1489..1573
                     /locus_tag="WIB82_001942"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1523..1525,aa:Leu,seq:tag)
     gene            2093..3127
                     /gene="purM"
                     /locus_tag="WIB82_001943"
     CDS             2093..3127
                     /gene="purM"
                     /locus_tag="WIB82_001943"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ3843191.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGVGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT          842 a          861 c          818 g          775 t
ORIGIN      
        1 ttattgcttt atcaaggtat ggaaacctgt ttcccgaaag gcggcgcagg atgcccgttc
       61 cctgcaactt tgccttattc cgacatcaga tgtcccagct tgtctgcttt ggtttggagg
      121 tagcgtttgt tttcaaggtt ttccccgacg tgcaggggga tgcgttcgac cacgttaatc
      181 cccgcatctt tcagggtttg gattttttcg gggttgttgg tcagcagttt gaccgagcgg
      241 atgcccagat attcgtagat agattgcgcc aaacggaaat cgcgggcatc gacgggcagc
      301 ccgagtgcca aattggcttc aaccgtatcc ataccttggt cttgcagatg ataggcgcgg
      361 attttgttaa tcagcccgat gccgcgtcct tcctgacgca gatagacgat gatgccgcgc
      421 ccctctgtct gtaccgccct catggccgct tcaagttgag gtccgcagtc gcattttctc
      481 gagaacagcg catcgcccgt caggcattcg gagtggatgc gcgtcagaac cggattgccg
      541 tctgaaaaat taccgacggt cagcgcgacg tgttcctgcc cgtttgcctc ttcaaagccg
      601 tgcatcgtaa atacgcccca ttcggtcggc aggcggcagg aagcaacata gtttaataat
      661 tcagacattt tcatctcctt ctgcggcagt gatgcccaaa agctgcttca aaggttcgga
      721 caaagccaag gcaagagcca cccattccac ctgtgctgcc gtacccgcac actctgcgca
      781 ttcaaattcc acatgaacca cgcccaacac accgccactt tccgtgcaga ccggaatgga
      841 aatttgcgcc gccgaagcat gattgcgttc tcccgaaagc tctcccaaat ccaaccaacg
      901 gcgtacatcc gaggcaacat tcatccagcc gctttgcgcg caacggcagg caagtgaaac
      961 atcggcaaca ttttcatcta gattccatat gttttccaaa ccttctccct gttgggacag
     1021 gcaaaccagc cgaaaggcgg cattttcaga cggcatcagg gcataaacgg ctgcggcgcg
     1081 cacgccttta cagcgcgaaa ataccgaatc cagagccatg aaaagccgtt tcagaccttc
     1141 ctcattcccg ctgttttgct ccaaataatc ggcaagcctc caatcctcat gctcaagcca
     1201 caaaaccgaa cggtctatcg aagccgtccc catatccatt accgtctgcg ccgtcagata
     1261 cgccgcccga acctcgtcaa gcggcagctt caaaccctga gtcagcagaa aatccttaat
     1321 caaagcagca ggcataccca aattccgtca ataaaaacaa aaaaccgccc gattccgatg
     1381 tcaggcggac gaaaaacaga ttttaccgcc ccaaacccca agccatcaac agccccgccg
     1441 aaaaccttgc acaaaaaaac aaaatctgta taatcgtcca attcccatgc ggacgtggcg
     1501 aaattggtag acgcaccaga tttaggttct ggcgccgaga ggtgtgagag ttcgagtctc
     1561 tccgtccgca ccaataatga taaataaatt atatagttaa tgcttatcgg gacaaattag
     1621 tgccacaaaa cataagtcgt ctgcttttca gcgggcggct tttttatctg cctatatgtt
     1681 tcaacacgca gaacggtaca taaagtgccg ccatttgttc cgcaccgaat cggaaacggg
     1741 tgcttccgca cgcaatcaga ttcaccaccc ctaatgccgg ggtttcaacc ggaaagaaga
     1801 aaatattgat tttgatgatg gcatcaaaaa accccttccg ctcgtctgcc aaaaggaatg
     1861 gttaagaaaa gcaatgcgtc ttttgatgga acgcgatata taaggaattg aggggaaggg
     1921 caggcgggtt tctgcccgtt aatcctgcgg acggtcccgc caaacccgac acgcgcgggc
     1981 atatggcgga ctgtttacaa tattgtctaa aaatctgaac ctgcaaacgt gaaataaggt
     2041 agaatacgcc ccgttatttt accgtccaat ccccatttac caaggaaaaa cgatgagtac
     2101 ttcattgagt taccgcgatg caggtgtcga tatcgacgca ggcgaccaac tggtcgaaaa
     2161 catcaaaccg tttgccaaac gcacgatgcg cccggaagta ttgggggatt tgggcggttt
     2221 tggcgcattg gtcgaaatcg gcaagaaata tcaaaatccc gtattggtca gcggtacgga
     2281 cggcgtgggt accaagctca agcttgcctt tgattgggat aaacatgata cggtcggcat
     2341 cgaccttgtt gcaatgagtg tcaacgatat tttggttcaa ggggctgagc ccttgttttt
     2401 cttggactat tttgcctgcg gtaaattgga tgttccgcgc gcgaccgatg ttattaaagg
     2461 cattgcccaa ggttgcgaag aatccggttg cgccctgatt ggcggggaaa ctgccgaaat
     2521 gccgggtatg tatcccgtcg gagaatacga tttggcgggt tttgccgtcg gcgtggtgga
     2581 aaaagagaat gtcattaccg gccgcagcat cggcgtaggc gatgtggtat tgggtttggc
     2641 ttccaacggc gcacattcaa acggctattc ccttatccgt aaaatcatcg aacgcgacaa
     2701 tcccgatctg gatgccgagt ttgataatgg caaaaccttg cgggaggctg ttattgcgcc
     2761 gacccgtctg tatgtgaaac cgattcttgc cgctttggaa aaatttacca ttaaaggtat
     2821 ggcacacatt accggcggcg gcattaccga aaacgtgccg cgcgtgttgc ctgaaaacac
     2881 ggttgcacaa atcgatgcta aatcgtggga attgcccaag ctcttccaat ggcttcaaaa
     2941 ggcgggcaat gtggaaaccc aagaaatgta ccgaaccttt aactgcggca tcggcatggt
     3001 cgttattgtt gctgccgaag atgccgatgc ggttcagggt ctcttgggtg aacaaggtga
     3061 aacagtttac cgtttaggtt tgatccgtga gcgtcaggga gacgagcatc aaacccaggt
     3121 tgcctgattg cttctatagc gaaatgccgt ctcaaacatg ggtttggacg gcatttttat
     3181 agaggatggc ggattcgcat ttgaagtgca actttcccta acagaaaaag gccagtatgc
     3241 ggtagcatac ggcctttcct gcaagaaaga ttgccatgag ctacacgcaa ctgacc
//
LOCUS       DAWHRS010000180         3252 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWHRS010000180 DAWHRS010000000
VERSION     DAWHRS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.180"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            142..1285
                     /locus_tag="WIB82_001944"
                     /pseudo
     CDS             142..1285
                     /locus_tag="WIB82_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(1395..1604)
                     /locus_tag="WIB82_001945"
     CDS             complement(1395..1604)
                     /locus_tag="WIB82_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843192.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1601..1897)
                     /locus_tag="WIB82_001946"
     CDS             complement(1601..1897)
                     /locus_tag="WIB82_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ3843193.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRRARL
                     AQKPKTRWKILYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1876..2481)
                     /locus_tag="WIB82_001947"
     CDS             complement(1876..2481)
                     /locus_tag="WIB82_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843194.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSDYHEWDGKKWKISKAAAAARFAKQKTALAFRLAEKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTLMLAQIAAARGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            2561..2848
                     /locus_tag="WIB82_001948"
     CDS             2561..2848
                     /locus_tag="WIB82_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ3843195.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2820..3122
                     /locus_tag="WIB82_001949"
     CDS             2820..3122
                     /locus_tag="WIB82_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ3843196.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYIAQLK"
BASE COUNT          713 a          858 c          858 g          823 t
ORIGIN      
        1 gcaacgctgt actggtttaa atttaatcca ctataaatta acacgcaatg taccctgacc
       61 ggcaaaactt ccccaaactt catcaaaaaa tcaactgtac ccgtcttttt cagacggcat
      121 tgataccgta agaaatttcc catgcagaaa tggcaaaccg agctttattc cacaccgctt
      181 tggctttgtc tgacaccgaa aagcgcggtc aaagtcttgc tgctgattac ggcgatgatt
      241 acgctggtgt taaccgaaat gcggctgaat gtgttgagta cctttatgtc caaagggctt
      301 tacgactcga tgcaggattt gaaatgcttc ggcattttgg atgtttgctg caatgaacgc
      361 gggcgtggtg ctgatacggg cgtttaacaa cgtcgtcaac gacttcctcg atcaaggctt
      421 ggcgattaaa tggtcggagc ggctcaacga agtgctgaca gcacgctggc ttgccgacaa
      481 aaactactac cgcctgcaaa tgcgccgcca cgcgccggac aacatcaacc agcgtatcca
      541 acaggacgcg caggaattta tcgcttcgac catcgaattt gtgcgcggca tggtcaattc
      601 ggtcgttact tctctggaat ttgccgttgt tttgcggggc ttggctggca tcctgaccgt
      661 gttcggcttc gacattccgc acggcatcgt ttggtttgtc tatatatttg tgattttggc
      721 gacctttatc gccatgtgga taggcaaccc tttgattcgt tacaattatg aaaacgaaaa
      781 actcaacggc gactaccgtt attccctcat ccgcgtgcgc gaccacgccg aaagcgtggc
      841 gttttacagc ggcgaaaaac acgaacacga ccagctttct gaccgtttta aagccattat
      901 ccgcaaccgc tggcgtattg cgcgacaaag cgtctgcctg agcggcttta atgatatgtt
      961 caccaacggc atcaagctct tccccattat tttgcaagcg ccgcgcctgt ttgccggaca
     1021 aatcaaaatc ggcgacatcc agcagaccgt ccaagccttc gcgcgactgc aaaacgcgct
     1081 ttctttcttc cggatgttct acaacaaatt caccgcctac cgcgcccgat tggagcgtct
     1141 gtacggcttt ttgctgagta ccgaagaaca acacagcgcg cggcaacccg agattagcga
     1201 agtttcagac ggcattgcac tggaatgtaa ggcggcggca gcttttgcac gaattggcgg
     1261 cagcttttgc accgccgaag cgtaggaaat tgtattagct tttgcacaaa tttatccccg
     1321 tttaatggtc tgttaaacgg ggattatctt atgcctttaa aagtagtacg ctacttgcgg
     1381 tcagaactta taaatcaggt ggtcgtcgta gcttgaattg ctgttgatgt gctgctcaca
     1441 gtcaaggttt ggctgcctgc aaaacgctcc catccggtca ggcgcagttc tgccacgagg
     1501 tcaagataag caggcagttc gctgcgggtg ccggagaaga agacaaatgg cggccgcacc
     1561 attgccatca ggcgcagaaa ctctaccatg ccgaagtagt tcatcgcgtc cccttttccc
     1621 cggcggcgcg gctttggttg aaccgcgcgt cgtgctgcct gcctttcagt tcgctgatat
     1681 acgcctgccg gcagtggtgg cggtcgaaaa acacgccgtt gatcagggta tataaaatct
     1741 tccagcgggt tttgggcttt tgggcgagcc gcgcgcggcg ataggtgcgg cttgagaggg
     1801 tttcgtccgc cgcgccgccg gttagggcgt tgaacagttg gtcgatggct accaacaggt
     1861 ggtaaacgta ttttttcagc cgatgtttag cgtccattct tcgatttcct tttccagcgc
     1921 gtccaatccg ggcgcggttt cgatggtgtt caatttgtct tcgagctgct gacgctttcc
     1981 gataatcgcg ccggcggcaa cagccaggcg ggcggatttt tcgataactt tttcaatcaa
     2041 aacgtccaat tccacgcccc ttgcggcggc gatttgcgcc agcatcaggg tcggggcgtt
     2101 gttgtccgcc tgccgcgcga gggcttcttt ttcctgcctg taaaagctgt cgatttccac
     2161 ttggggatag cccgccaaga ggctgttttt gagttcgtcc gccttttccg cgaggcggaa
     2221 tgccaaggcg gttttttgtt tggcgaaacg ggcggcggcg gcggctttgc tgattttcca
     2281 ttttttgccg tcccattcgt ggtaatcgga cgggcgcggc ggggttaaaa cggggcggcc
     2341 gtcggaatct gcggcaatct gcccgccctg cgcctgtcct gccaaaaggg cggcgtattc
     2401 ttcggcgcgg acggcaaccg cgccttcggg gatgccgccc aatgtgtcgt cgtaaaagcc
     2461 gtttttgaaa tagatggtca ttttggggtt tccttttttg tatttacatt ttggtttttt
     2521 gccgcttata atgtaacgac aaaatacgct gcaagctgca atgaagattg aatttgactc
     2581 cgaaaaaaat caacgcaata tcgaagagcg gaatttgccc tttgaatcag tcgggcaaat
     2641 acgctggaca acggccgtta ttgtgcccga tgtccgcttt gattaccccg aaccgcgtta
     2701 tgttgccgct gcttatctag gcgatacgca acgcctgcac attgtctgtt ttacgccgat
     2761 aaaagacggc atccgtgtca tcagttttcg caaagcgaat aaaagagagg ttaaaaaata
     2821 tgcaacgtca atccttaaca aacgctgacg gcgaagtgcg ggaacttgcc gccgaagatt
     2881 ttgcgcttgc ccgccccatt gccgaagccc tgcccgaaga cttggcacag gtgctttttt
     2941 cccatcaaaa acaattggaa gaaaagggcg taatgcaaaa acgaaacaca ggcaaaaccc
     3001 cgaaacaact cgttaccata cgcctgtccg ccgatgtggt cgaaaaattc cgcgccggcg
     3061 gcaagggctg gcagacgcgc atcaacgagg ttttgcgcca atatatcgcc caattgaaat
     3121 aacgggggcg gccgctgctt atcgggcgcg cccgactatc ctttcccgcg cggtttcggc
     3181 cgtgcggttt tttgttgccg gtgccgtccg aagggggcag cggctttttg cgcggtcatt
     3241 cccgcctttc gt
//
LOCUS       DAWHRS010000181         3236 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWHRS010000181 DAWHRS010000000
VERSION     DAWHRS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.181"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            21..482
                     /locus_tag="WIB82_001950"
     CDS             21..482
                     /locus_tag="WIB82_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843197.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAAFWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVASIGTFAGKNKERKAASAADGTKNDV"
     gene            472..1293
                     /gene="prmC"
                     /locus_tag="WIB82_001951"
     CDS             472..1293
                     /gene="prmC"
                     /locus_tag="WIB82_001951"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ3843198.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            1379..2767
                     /locus_tag="WIB82_001952"
     CDS             1379..2767
                     /locus_tag="WIB82_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ3843199.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLTGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            2768..2992
                     /locus_tag="WIB82_001953"
     CDS             2768..2992
                     /locus_tag="WIB82_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ3843200.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDTQEPYSLRDEIPPHY"
BASE COUNT          674 a          786 c          981 g          795 t
ORIGIN      
        1 ttttacatta ggataaaacc atgcaggaac agaatcggaa atcaagtttt cccatagtga
       61 tgttgctggt gtcggttgcc ctgtggatag cgtctttatc caatgttgca ttttatttgg
      121 gcaatcatgg aagcatggag ggtttgaccg ttttgatttt ggggtcgata tttgcttctt
      181 tggatatcag gtattgtgcg gtctatgcga attatgtttg gttggcggcc attgttttgc
      241 tggcgttgcg gaagaaggtc gtgcctgtcc atgcggcatt ttggggcttg gcgttggtgg
      301 ctttcagtgt gaaagccgta tacgtcgatg aagcagggaa tacatcggat attgtgcgct
      361 acggtgcagg attttatttg tggtatgccg catttgcggt tgcctccatc ggtacgtttg
      421 ctggaaagaa taaggaaaga aaagccgcat cggcggcaga cgggacgaaa aatgacgttt
      481 gataaatggt tgggcttgtc aaaactgcct aaaaatgaag caagaatgct gctgcaatat
      541 gtttcggaat atacgcgcgt gcagttgttg acgcggggcg gggaagaaat gccggacgaa
      601 gtccgacagc gggcggacag gctggcgcaa cgccgtctga acggcgagcc ggttgcctat
      661 attttaggtg tgcgcgaatt ttatggcaga cgctttacag tcaatccgag cgtgctgatt
      721 ccgcgccccg aaaccgaaca tttggtcgaa gccgtattgg cgcgcctgcc cgaaaacggg
      781 cgcgtgtggg atttggggac gggcagcggc gcggttgccg taaccgtcgc gctcgaacgc
      841 cccgatgcgt ttgtgcgcgc atccgacatc agcccgcccg cccttgaaac ggcgcggaaa
      901 aatgcggcgg atttgggcgc gcgggtcgaa tttgcacacg gttcgtggtt cgacaccgat
      961 atgccgtctg aagggaaatg ggacatcatc gtgtccaacc cgccctatat cgaaaacggc
     1021 gataaacatt tgttgcaagg cgatttgcgg tttgagccgc aaatcgcgct gaccgacttt
     1081 tcagacggcc taagctgcat ccgcaccttg gcgcaaggcg cgcccgaccg tttggcggaa
     1141 ggcggttttt tgttgttgga acacggtttc gatcagggcg cggcggtgcg cggcgtgttg
     1201 gcggagaatg gtttttcagg agtggaaacc ctgccggact tggcgggttt ggacagggtt
     1261 acgctgggga agtatatgaa gcatttgaaa taattgtttg caaaattggc gggttttcca
     1321 ttattgggtt atgatgtgtt tcgatatgta aacatttata ggtttggagc gataaacaat
     1381 gaatgcagtc gtagttgccg taatcgttat gctggtgctg tcgctgtcgc gcgtgcacgt
     1441 ggtattgagc ctgacggtcg gcgcgtttgt cggcggcgcg gtggcgggta tgccgctgca
     1501 aaacattgcc gatgcggcgg gacaggtcag tcaggcgggg attatccccg tgttcaacaa
     1561 aggtttggaa ggcggtgcga agattgcgct ttcttatgcg atgctcggcg cgtttgcaat
     1621 ggcgattacc cattccggcc tgccgcagca gcttgccggc gcggtcgtcc gcaagctgaa
     1681 ccggggcggt atgcccgaca gcgtgcgttc gggcgagggc gcggtcaaat ggctgctgct
     1741 ttccatcatc cttgtgatgg gcattatgag tcagaacatc atacctatcc acatcgcctt
     1801 tatcccgatg attgttccgc cgctgctttt ggtgttcaac cgcctgaaaa tcgaccgccg
     1861 tctgattgcg tgcgtcatta ctttcgggct ggttacgacc tatatgttcc tgccttacgg
     1921 cttcggcgcg atttttttga acgaaatcct gttgggcaac atccattccg ccgcgccgca
     1981 gcttgatgtg aaaaacatca acgtaatggc ggcaatggcg attcccgcgt tgggaatgct
     2041 gaccggactc ctgctggcgt ttgtccatta ccgcaaaccg cgcctgtacc aaagcaacaa
     2101 tgccgatacg gcgggcaacg ccgatgcggc aaaccgtccg cagccgtccg cctaccgcag
     2161 cctggccgcc gccgccgcca ttgccgtatg ttttgccatc cagttgatgt atgaagactc
     2221 gctggtgttg ggcgcgatgc tcggtttcgc cgtatttatg atgttggggg tcatcaaccg
     2281 cgacaaggca aacgacgtat tcggcgaagg tatcaagatg atggcgatgg tcggcttcat
     2341 tatgattgcc gcgcagggtt ttgccgccgt gatgaatgcg accgggcata tccagccgct
     2401 ggtggaaagc agtatggcga tattcggcaa cagcaaaggt atggcggcat tggcgatgct
     2461 ggtggtgggg cttttggtaa cgatgggcat cggttcgtcc ttttccactt tgccgattat
     2521 tgccgcgatt tatgtgcctt tgtgtgtcgg tttgggtttt tcgccgcttg ccaccgtcgc
     2581 cattgtcggc acggcggggg cgttgggcga tgccggttcg cctgcgtccg attccacgct
     2641 gggcccgacg atggggctga acgccgacgg gcagcacgac cacatccgcg attccgttat
     2701 cccgaccttc atccactaca atatcccgct gctgattgcc ggctggattg ccgcgatggt
     2761 gctgtaaatg gacgcggttc aagagttgga acaccgcatc acagagctgg aaatccaatc
     2821 cgcgcttcag gaggacgtaa tcgccggcct gaacgcgatg gtggcggaat tgcggcagac
     2881 gctggatttg cagcaggctc agttgaggct gctgtatcaa aaaatgcagg acaggaatcc
     2941 cgacacgcaa gagccgtatt ccctgcgcga cgagattccg ccgcattatt gatgtgccgc
     3001 cgtatccgga tttccttaaa aaaggctgtg tttgaatatt ttgccgatgc aaacctaaga
     3061 tgtaacttta atcagtcgga caaaatgcct tttatccgat ggaaccgttt tccgcccgaa
     3121 cggaaataag cggttttccc ctgtaaacag ataaaaccaa ccgggtattc atacacagcc
     3181 aaaaaaaagc cgtccgaacc caaaggggac agacggccaa acacattacg gggaaa
//
LOCUS       DAWHRS010000182         3227 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWHRS010000182 DAWHRS010000000
VERSION     DAWHRS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.182"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(120..1034)
                     /locus_tag="WIB82_001954"
     CDS             complement(120..1034)
                     /locus_tag="WIB82_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ3843201.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WIB82_001955"
     CDS             1290..2759
                     /locus_tag="WIB82_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ3843202.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..>3227
                     /locus_tag="WIB82_001956"
     CDS             3011..>3227
                     /locus_tag="WIB82_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843203.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEW"
BASE COUNT          682 a          726 c          933 g          886 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggttg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtggg
//
LOCUS       DAWHRS010000183         3162 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWHRS010000183 DAWHRS010000000
VERSION     DAWHRS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.183"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            147..1157
                     /gene="dusB"
                     /locus_tag="WIB82_001957"
     CDS             147..1157
                     /gene="dusB"
                     /locus_tag="WIB82_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ3843204.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFVDEGGIVAVQIAGSNPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVSAAGVPVTLKTRLGWHDDHKNLPVVARIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRPN
                     AF"
     gene            1187..1426
                     /locus_tag="WIB82_001958"
     CDS             1187..1426
                     /locus_tag="WIB82_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ3843205.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            1429..1965
                     /gene="ruvC"
                     /locus_tag="WIB82_001959"
     CDS             1429..1965
                     /gene="ruvC"
                     /locus_tag="WIB82_001959"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ3843206.1"
                     /translation="MSATVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LASRLNPSGMQVKRGRFQ"
     gene            2016..2885
                     /locus_tag="WIB82_001960"
     CDS             2016..2885
                     /locus_tag="WIB82_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ3843207.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKGSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREEDNTVTLHFYPAWESFPSEDAQADAQRMNRFIEDRVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
BASE COUNT          759 a          821 c          860 g          722 t
ORIGIN      
        1 tgcccgcctg tgagtggcgg ggcagggaat ctgtgtgcgg attatgccat aaaacggtgt
       61 ccgacccaat cgcgggcgcg tccggagatt ggaaatcctg cttaaaaaat gtacaatggc
      121 gtactttttt gaaacgcggg cccattatgc acatcggcgg ctattttatc gacaacccca
      181 tcgcacttgc accgatggcg ggcattaccg acaaaccctt ccgccgcctc tgccgcgatt
      241 tcggcgcggg ttgggcggtg tgcgaaatgc tgaccagcga cccgacgctc agaaatacta
      301 gaaaaacctt gcaccgcagt gattttgtcg atgaaggcgg catcgttgcc gtgcagattg
      361 ccggcagcaa tccgcagcag atggcggatg ccgcgcgtta taacgtcagc ctcggagcgc
      421 aggtcatcga catcaacatg ggctgtcccg ctaaaaaagt ctgcaatgtc caagccggta
      481 gcgcgctgat gcaggacgag ccgctggttg ccgccatttt ggaagccgtc gtcagtgcgg
      541 caggcgtacc cgttactctc aaaacccgtt tgggttggca cgacgaccac aaaaacctgc
      601 ctgtcgtcgc ccgcatcgcc gaagattgcg gcattgccgc ccttgccgtc cacggacgca
      661 cgcgcacgca aatgtacaaa ggcgaagcgg cttacgacct gattgccgaa accaaatgcc
      721 gtctgaacat cccggtttgg gtcaacggcg acattacttc gccgcaaaaa gccgccgccg
      781 tcctcaaaca aaccgccgcc gacggcatca tgatagggcg cggcgcgcaa ggcagaccgt
      841 ggttcttccg cgatttgaaa cattacgccg aacacggtgt tttaccgcct gccttgagtt
      901 tggcagaatg cagtgccgcc attttgaacc acgtccgcgc catgcacgcg ttttatggtg
      961 agaccgccgg tgtgcgcatt gcacgcaaac acataggctg gtacatcgac gaaatgcccg
     1021 acggcgaaca gacacgtcgt gaaatcaacc gcttggacag tgcggcggcg caatacgaca
     1081 tgcttgcagg ttatcttgaa agacttgccg gaaaaaccga ccgttgggcg tgcgcctacc
     1141 gcccaaatgc cttctgaaca cttgattatc ctttgaaagt gcaatcatgc cccataccct
     1201 tcccgatatt tcccaatgta tcagacaaaa tttggaacaa tatttcaaag acctgaacgg
     1261 taccgaacct tgcggcgtgt acgatatggt attgcatcag gtggaaaaac cgctgctggt
     1321 gtgcgtgatg gaacaatgcg gcggcaacca gtccaaagcc tccgtcatgt tgggactgaa
     1381 ccgcaatacc ctgcgtaaga agctgattca acacggtttg ctgtgaatat gtcggcaacc
     1441 gtccgtatct tgggtattga cccgggcagt cgcgtaacgg gtttcggtgt catcgatgtc
     1501 agggggcgcg atcattttta cgtcgcctcc ggctgcatca aaacgcctgc cgatgcgcct
     1561 ctggcagaca ggattgccgt gattgtgcgg cacatcggcg aagtcgttgc cgtttacaag
     1621 ccgcagcagg cggcggtgga acaggtgttc gtcaacgtca atcctgcatc gacgctgatg
     1681 ctcggtcagg cgcggggcgc ggcattggcg gcattggtca gccataagct gcccgtttcg
     1741 gaatacacgg ccttgcaggt caaacaggcg gtagtcggca aaggcaaggc ggcaaaagaa
     1801 caggtgcagc atatggtggt gcagatgctg gggctttcgg gaacgccgca ggcggatgcg
     1861 gcggacggtc ttgccgtcgc gctgacccac gccttacgca accatgggct tgcgtcccgg
     1921 ctcaacccgt cggggatgca ggtcaagcgc ggaaggtttc aatagtttca gacggcattt
     1981 gtattttgcc gtctgaaaag aaaatgtgta tcgagatgaa atttatattt tttgtactgt
     2041 atgttttgca gtttctgccg tttgcgctgc tgcacaagat tgccgacctg acgggtttgc
     2101 ttgcctacct tctggtcaaa ccgcgccgcc gtatcggcga aatcaatttg gcgaaatgtt
     2161 ttcccgaatg gagtgaggaa aagcgtaaaa ccgtgttgaa acagcatttc aaacatatgg
     2221 cgaaactgat gctcgaatac ggcttatatt ggtacgcgcc cgccgggcgt ttgaaatcgc
     2281 tggtgcgcta ccgcaataag cattatttgg acgacgcgct ggcggcgggg gaaaaagtca
     2341 tcatcctgta tccgcacttt accgcgttcg agatggcggt gtacgcgctt aatcaggatg
     2401 ttccgctaat tagtatgtat tcccaccaaa aaaacaagat attggacgaa cagattttga
     2461 aaggccgcaa ccgttatcac aacgttttcc ttatcgggcg caccgaaggg ctgcgcgccc
     2521 tcgtcaaaca gttccgcaaa ggcagcgcgc cgtttctgta tctgcccgat caggatttcg
     2581 gacgcaacga ttcggttttt gtggattttt tcggtattca gacggcaacg attaccggat
     2641 tgagccgcat tgccgcgctt gcaaatgcaa aagtgatacc cgccattccc gtcagggagg
     2701 aggacaatac ggttacattg catttctacc ctgcttggga atcctttccg agtgaagatg
     2761 cgcaagccga cgcgcagcgc atgaaccgtt ttatcgaaga cagggtgcgc gaacatcccg
     2821 agcagtattt ttggctgcac aagcgtttta aaacccgtcc ggaaggcagc cccgattttt
     2881 actgactacg taaaattaca aaacatatca ggcgtttcaa gtcgaaattc ctgattgttt
     2941 ttggaaattt gaaatccggg tcgtgcaaac aggatttgcc ggacggtttt aacggttcag
     3001 ttgtttgtaa aaacaatgct ttttaagatt gacaaaaaac cagaatcagg ttttagggct
     3061 tattccgcga acaaaggggg cggatgccga aaatccgatt aatatattaa aattaaattt
     3121 gtttaaaaac ataaagttgt aaacaagtat ctcatataag cc
//
LOCUS       DAWHRS010000184         3029 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWHRS010000184 DAWHRS010000000
VERSION     DAWHRS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.184"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..155
                     /locus_tag="WIB82_001961"
     CDS             <1..155
                     /locus_tag="WIB82_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MutL dimerization domain protein"
                     /protein_id="HEZ3843208.1"
                     /translation="RAGRRLTLPEMNALLRDMENTPRSNQCNHGRPTWVKLTLKELDA
                     LFLRGQ"
     gene            250..2364
                     /gene="dnaX"
                     /locus_tag="WIB82_001962"
     CDS             250..2364
                     /gene="dnaX"
                     /locus_tag="WIB82_001962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ3843209.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPAALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELKSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAEPVTNQENNDIPPWEDAPDETAAGTAQA
                     SAKSIQTASEAGTPPKNQVSKNEAADNETDAPLSEVPSENPIQATPNNEALETEAFAH
                     EAPAKPFNGYSFPNDDYLVEDGAEIPPPDWEHAAPADAEEENNADESSNNEDHTPYAP
                     PPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATADKKR
                     LDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADPAAQ
                     KILQAFGAQWQPESLELAANRP"
     misc_feature    1439..1478
                     /gene="dnaX"
                     /locus_tag="WIB82_001962"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            2444..2779
                     /locus_tag="WIB82_001963"
     CDS             2444..2779
                     /locus_tag="WIB82_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ3843210.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
BASE COUNT          862 a          983 c          728 g          456 t
ORIGIN      
        1 tccgcgccgg ccgccggctc accctgcccg aaatgaacgc ccttctgcgc gatatggaaa
       61 atacgccgcg cagcaaccag tgcaaccacg gcaggccgac ttgggtcaaa ctgactttga
      121 aagaattgga cgcactgttc ttgcgcggac agtaagccga aagtgctaga atacgccgcc
      181 cgagaccgcc gttcagacgg cattccgacg caccgacaga aacatcacga ccgaaaccaa
      241 gagaaaaaca tggcctatca agttctcgcc cgaaaatggc ggcccaaaac ctttgccgac
      301 ttagtcggtc aggaacacgt cgtcaaagcc ctgcaaaacg ccctggacga aggcaggctg
      361 caccacgcct acctgctgac cggcacgcgc ggcgtaggta aaaccaccat cgcccgcatc
      421 cttgccaaaa gcctcaactg cgaaaacgcg caacacggcg aaccttgcgg cgtatgtgaa
      481 agctgtacgc agatcgatgc cggacgctac gtcgacctgc tggaaatcga cgccgcctcc
      541 aacacaggca tcgacaacat ccgcgaagtc ttggaaaacg cccaatatgc accgaccgcc
      601 ggaaaataca aagtctatat catcgacgaa gtgcatatgc tttccaaaag cgcgttcaac
      661 gctatgctca aaacgctgga agagccgccc gaacacgtca aattcatcct cgccaccacc
      721 gatccgcaca aagttcccgt taccgtcttg agccgctgcc tgcaattcgt cttacgcaat
      781 atgaccgcgc aacaggttgc cgaccacctc gcccacgtcc tcgacagcga aaaaatcgcc
      841 tacgaacccg ccgccctgca acttttggga cgtgccgccg ccggatcgat gcgcgatgcc
      901 ttgagcctgc tcgaccaagc catcgcccta ggttcgggca aagttgccga aaacgatgtc
      961 cgccaaatga tcggcgcggt tgacaaacaa tacctttacg aactgctgac aggcatcatc
     1021 aaccaagacg gcgcagccct gaccgccaaa gcgcaggaaa tggcggcgtg tgccgtcggc
     1081 tttgacaacg ccttgggcga acttgccata ctgctgcaac acctcgccct gatacaggca
     1141 gtgccgaatg ccttggcgca cgacgacccc gattccgata ttttgcaccg cctcgcccaa
     1201 accataagcg gcgaacaaat ccagctttac taccaaatcg ccgtccacgg caaacgcgac
     1261 ctcagcctcg cccccgacga atacgccggc tttatgatga ccctgctgcg tatgctggcg
     1321 tttgcgccct tggcggcagc atcgtgtgat gcaaatgccg tgattgaaaa taccgaacta
     1381 aaatccccat cggcacaaac cgccgaaaag gaaaccgccg caaaaaagcc ccaaccgcgc
     1441 cctgaagcgg aaaccgccca aacacccgtt cagacggcat ccgcagcagc aatgccgtct
     1501 gaaggcaaaa ctgccgaacc cgttaccaat caagaaaaca acgatattcc gccttgggaa
     1561 gacgcgccgg acgaaaccgc agccggcacg gcgcaagcat cggcaaaaag cattcagacg
     1621 gcatccgaag ccggaacgcc gcccaaaaac caagtttcca agaacgaagc agccgacaac
     1681 gaaaccgatg cccccttgtc cgaagtgccg tctgaaaacc ccattcaggc aacaccgaat
     1741 aatgaagccc ttgaaacaga agcatttgca cacgaagctc ctgcaaaacc tttcaacggt
     1801 tacagctttc cgaatgatga ctacctcgta gaagacggcg cagaaatccc accgcccgat
     1861 tgggaacacg ccgcccctgc cgatgcggaa gaagaaaaca acgccgacga aagcagcaac
     1921 aacgaagacc acacgccata cgccccgccg cccgaatttt ccaccgaaaa ctgggcagcc
     1981 atcgtccggc acttcgcccg caaactcggc gcggcgcaaa tgccggcgca acactccgcg
     2041 tggacggaat accatcccga caccggtctg atggttttgg caatgaccgc cgaagcacgc
     2101 gccaccgccg acaaaaaacg cctcgacaaa atccgcgaca cccttgccca agcctacggg
     2161 ctgcaactca ccctgcaaac ccaagactgg cgtgacgaag ccggccggga aacccccgcg
     2221 atgcaggaca agcgcgtcca agccgaagac aggcaaaaag cacaagcatt gctcgaagcc
     2281 gaccccgccg cacaaaaaat cctccaagca ttcggcgcgc aatggcagcc cgaatcactg
     2341 gaattggcgg caaaccggcc ataaacagat ataatgccgc ccgaaccctt cggacggcat
     2401 tgccgtttcc cttattcaat caaaacagac aggagtattc agtatgttcg gaaaagccgg
     2461 attaggcggc ctgatgaaac aggcgcagca aatgcaggaa aatatgaaaa aagcgcaagc
     2521 caaactcgcc gaaaccgaaa tcgaaggcga agcaggcaac ggcctggtca aaatcacaat
     2581 gacctgcgcg cacgaagtac gcaaaatcga catcagcccc gatttgattc aagaagccgc
     2641 cgacgacaaa gaaatgcttg aagacctcat cctcgccgcc ctcaaatccg cccgaggcaa
     2701 agccgaagaa accgcaaaca aaacaatggg cgcattcacg caaggtctac cccccggagt
     2761 gggcgacttc ttccgctgat ccccgaccgt cattcccacg caggcgggaa tctagaacgt
     2821 agaatctaag aaaccgtttt actcgataaa tttccgtgcc gaggggtctg gattcccgcc
     2881 ttcgcgggaa tgacggcatc agtttgcaag attcggcgtg aacggtaaaa acagtgagaa
     2941 tgataagaac gcaaaaacgg caagaatagc gggaatcggc aggctgaagc ccaccctacc
     3001 attatttaca catccgtacc gcttaaatg
//
LOCUS       DAWHRS010000185         2915 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWHRS010000185 DAWHRS010000000
VERSION     DAWHRS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.185"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(140..1132)
                     /gene="pdxA"
                     /locus_tag="WIB82_001964"
     CDS             complement(140..1132)
                     /gene="pdxA"
                     /locus_tag="WIB82_001964"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ3843211.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGASEEGVLEVLHIPAAEAVEAGKLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLNKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1292..2719
                     /locus_tag="WIB82_001965"
     CDS             1292..2719
                     /locus_tag="WIB82_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ3843212.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          580 a          794 c          834 g          707 t
ORIGIN      
        1 gaatattgcc gctgtccaat ggtctggatt cccgcctgcg cgggaatgac gatttggaaa
       61 ttacccgaaa cccaaaaaca actgaaaccg aacagaccgg attcccgcct gcgcggaaat
      121 gacgggtctt ttatcatctt taaaggctgc cgcgcgccat ctcgacggcg gtctccacgg
      181 cagttatcag gctgccggaa tccgccctgc cggttgccgc caaatcaagc gcggtgccgt
      241 gatcgacgga ggtgcggata aagggcaggc cgagcgtgat gttcacgccc tgtccgaagc
      301 tgtggtattt caacacgggc agcccttggt cgtggtacat cgccaatacg gcatccgcac
      361 cttcgagcat aaacggttgg aacaaggtat ccgccggata cgggccggcg aggtttatcc
      421 cttcgcggcg caggttttcg agcgcgggga taatggtgtc ggtttcttcg tgtccgaggt
      481 gtccgccttc gccggcgtgg gggttgagtc cggcgactag gattttggga tttttgatgc
      541 cgaatttgtg ttttaagtcg tgatgcaaaa tgcgtgcgac gctttcaatc agcggttgcg
      601 tgatggcggc ggcaacgtct ttcagcggca ggtgggtcgt tacgagggcg acgcgcaggc
      661 ctttgccggc aagcatcatc acaacctgcc ccgtgccgct tttttccgcc agatattcgg
      721 tgtgtccgct gaaaaaacct gtgcttgcgc gcgcgtcgtt gatgatgcct ttgttcagcg
      781 gcgcggtaac gatgccgtcg aaaatgccgt ctgaaatgcc tgcgagcgcg gtgtctagaa
      841 gttgcagcac ataggcggcg ttggcgggat tgagtttgcc cgcctcaacc gcttcggcgg
      901 cagggatgtg cagcacttcc agcacgcctt cttcggacgc gccgcctgat tccggatcga
      961 agtcgcgcag gacgacgctt ttgcccaagg cttcggcgcg cgcgcgcaac aggtttttgt
     1021 cgcccaatac cgcgcagcgg cagggcaggc gtgcaaatgc caagtccaaa caaatatcgg
     1081 ggccgatgcc ggcaggctcg ccggaagtaa cggcaaaaac aggctgtttc atcgtgtttg
     1141 ctccaaacaa aatgcgattc taacgccgca gccgcgcggc gatgtaaatt tttctgattt
     1201 tgttgacaat ctgctagaat gggcgtttac aaaatttaaa ccctgcttgc ataccgccaa
     1261 tatgtgcgag tttcaacttt aaggaagcga tatgaacgag aactttaccg aatggctgca
     1321 cggctgggtc ggcgccatca acgatccgat gtggtcatac ttggtttata tgcttttggg
     1381 tacggggctt ttcttcaccg taaccacggg ctttgtccaa ttccgcctgt tcgggcgcag
     1441 catcaaagaa atgctcggcg gccgcaaaca gggggacgac cctcacggca tcacgccgtt
     1501 tcaggcattt gtaaccggcc ttgccagccg cgtgggcgtg ggcaatatcg cgggcgtggc
     1561 catcgccatc aaagtcggcg gaccgggcgc ggtgttttgg atgtgggtaa ccgccttaat
     1621 cggtatgagt tcggcgtttg tcgaatcttc gctggcgcag ctctttaaag tccgcgacta
     1681 cgacaaccac catttccggg gcggccctgc ctactacatc actcaagggc tggggcagaa
     1741 atggctgggc gtgttgttcg ccctgagcct gattttctgt ttcggctttg tgtttgaagc
     1801 ggttcagacc aataccatcg ccgataccgt caaagcggca tggggttggg agcctcatta
     1861 tgtcggcgtc gccctggtga ttttaaccgc gccgattatc ttcggcggca tcaggcgcat
     1921 atctaaagcg gcggaaatcg tcgtccccct gatggcggtt ttgtacctct ttatcgcgct
     1981 tttcatcatt ttgaccaata ttccgatgat tccggacgtg ttcggtcaga ttttttcggg
     2041 cgcgttcaaa ttcgacgcgg cagcaggcgg cttactcggc ggtctgattt cgcaaacgat
     2101 gatgatgggc atcaaacgcg gcctgtattc caacgaggcg ggtatgggtt ccgcgccgaa
     2161 cgccgccgcc gccgccgaag tgaaacaccc tgtttcgcaa ggtatgattc aaatgctggg
     2221 cgtgtttgtc gataccatca tcgtttgttc ttgcaccgcc ttcatcatct tgatttacca
     2281 acagccttac ggcgatttga gcggtgcggc gctgacgcag gcggcgattg tcagccaagt
     2341 ggggcaatgg ggcgcgggct tcctcgccgt catcctgttt atgtttgcct tttccaccgt
     2401 tatcggcaac tatgcctatg ccgagtccaa cgtccaattc atcaaaagcc attggctgat
     2461 taccgccgtt ttccgtatgc tggttttggc gtgggtctat ttcggcgcgg ttgccaatgt
     2521 gcctttggtc tgggatatgg cggatatggc gatgggcatt atggcgtgga tcaaccttgt
     2581 cgccatcctg ctgctctcgc ccttggcgtt tatgctgctg cgcgattaca ccgccaagct
     2641 gaaaatgggc aaagaccccg agttcaaact ttccgaacat ccgggcctga aacgccgtat
     2701 caaatccgac gtttggtaaa tcccgccctt accggagccg cttccccgcg aagcggcttt
     2761 tccctttccg cacactgtaa aaacagggcg aacaagcgta caatcccaac cctttacttt
     2821 tgaatccatt tcgtttttca gacggcatat tgaatatagt ggtttaacaa aaatcaggac
     2881 aaggcgacga agccgcagac agtacaagca gtacg
//
LOCUS       DAWHRS010000186         2893 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWHRS010000186 DAWHRS010000000
VERSION     DAWHRS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.186"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(96..572)
                     /gene="greA"
                     /locus_tag="WIB82_001966"
     CDS             complement(96..572)
                     /gene="greA"
                     /locus_tag="WIB82_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ3843213.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VIYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            968..2269
                     /gene="aroA"
                     /locus_tag="WIB82_001967"
     CDS             968..2269
                     /gene="aroA"
                     /locus_tag="WIB82_001967"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ3843214.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2312..2845)
                     /locus_tag="WIB82_001968"
     CDS             complement(2312..2845)
                     /locus_tag="WIB82_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ3843215.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          602 a          793 c          786 g          712 t
ORIGIN      
        1 tatctgtact gtcttcggct tcgtcgcctt gtcctgattt ttattaatcc actataattt
       61 cctgcgtgtg tcgggtgtat cgaaatcaag ccgaatcaaa tatatcggac ttcgataatg
      121 tcgtattcgc gcacgccgcc cggggcttgg acttccgccg tatccccctc ttccttgccg
      181 attaaggcgc gggcgatggg tgagccgaca tagattttgc cctgtttgat gtcggcttcg
      241 tcttcgccga caatttgata gataacgtgt tcttccgttt ccaaatcttc cagcgtaacc
      301 gtcgtaccga acacgatttt gccttcggcg tggatttcgg tcggattgat gatgtgggca
      361 acggaaagtt tgtgttccag ctcggaaatg cggccctcga taaagccttg gcgttctttg
      421 gcggcttcgt attcggcgtt ttcggacaaa tcgccgtgcg aacgggcttc ggcaatcgct
      481 tcgatcactt cgggacgcgc cacgcttttg agctgctgca attcctgttt cagcaattcc
      541 gcaccgcgta cggtcagggg gattttttgc atcggtcttt tttctccata ttccggcaca
      601 ccggtttgcg gcagcaagca taccgcgtac cgtcttgttt tgtgcgtccg gatattaaaa
      661 taaaaataca agccgcccgg aaaatcggcg gcttgtctgt cgttgaacag cggctattct
      721 accaaattct atgaaattgg caatcgtgcc gtgccgccgg caaacgcgcc atgtccgcaa
      781 caaaagctga aaatatgccg tcaaagaaat tttagaaaca aaaaatttaa aaataatcaa
      841 ttttcagcat aaaaaaccac atttacggac tttaaaaccg aaaatgccaa gcctgagatt
      901 tttcatacag catttgcacc agtataatgc aggctgtttt tatctttaat aatattgacg
      961 ttttgccatg accgaatccg tccgcctccc cgtcgcccgt ctcaaacctt ccaccgtcgc
     1021 cctgcccggc tccaaaagca tcagcaaccg caccctgctg cttgccgcct tgtccgacaa
     1081 tgcttgcgaa atccattccc tgctcaaatc cgacgatacc gaccgtatgc tcgaagcact
     1141 cgataaactc ggcgttcaaa tcgaatatct tgccgaagac cgtctgaaag tgcacggcac
     1201 aggcggacgc ttccccaacc gcactgccga tttgtttttg ggcaacgcgg gcacggcgtt
     1261 ccgcccgtta accgccgctc tggccgtttt gggcggcgat tatcatctgc acggcgtgcc
     1321 tcgtatgcac gaacgtccta tcggcgattt ggtcgatgcg ttgcggattg ccggggccga
     1381 tgtcgaatat ctcggcaagg aacactatcc gccgcttcat atcggcgaac gccaagacaa
     1441 cggcgagcgc gtgattccga ttaaaggcaa tgtgtccagc cagtttctga ccgccctttt
     1501 aatggcgttg ccgctgaccg ggcaggcgtt tgaaatccgt atggtcggcg aattgatttc
     1561 caagccctat atcgacatta ctttaaaact gatggcgcaa ttcggcgtac aggttatcaa
     1621 tgaaggctac cgcgtcttca aaattcccgc cgatgcgcac taccacgcgc ccgaacactt
     1681 gcacgtcgaa ggcgatgcct ccagcgcgtc ctacttcctc gcagccggtt tgattgccgc
     1741 cacgcccgtc cgcgttaccg gtatcggcgc aaacagcata cagggcgatg tcgcctttgc
     1801 ccgcgagctg gaaaaaatcg gggcggacgt ggtttggggc gaaaacttcg tcgaagtttc
     1861 acgcccgaag gaacgtgccg tccaatcctt tgatttggat gcgaaccata tccccgatgc
     1921 cgccatgacc ctcgccatcg tcgcgcttgc tacaaggcaa acctgcacgc tgcgcaacat
     1981 cggttcgtgg cgcgtcaaag aaaccgaccg catcgccgca atggcaaacg agttgcgcaa
     2041 actcggggca aaagtcgtcg aagaagccga agcaattcac atcaccccgc ccgaaacgct
     2101 gacacccgac gccgtcatcg acacgtacga cgaccaccgc atggcgatgt gtttctcgct
     2161 ggtttcgctg ttgggcgtac ccgtcgtcat caacgatccg aaatgcaccc acaaaacctt
     2221 cccgacttat ttcgacgtgt tctcatcgct gaccgaaaca gcggaataag gcggcatttt
     2281 gccgcgattc cggcgcggcg cggcgggcgg ctcattctgt aaaaaaagta tgtgcgccga
     2341 ggtagttttt ggcgtaaaac ggcgtggaga gtttttcggt tttgatggtt ttgccgctgc
     2401 tgggggcatg gatgaattcg ccgttgccga tgtagagtcc gacgtgtgag tagcggtgtg
     2461 cgccgccggt gttgaagaat acgaggtcgc cggccttgag gcggctgtcg ggaattttgc
     2521 ggcttgccgc cgccatgtca cgggcggtgc gcggcagctt gacgttgagg gcgtttttgt
     2581 aaacgaattg aatcatgccg ctgcaatcga agccggttgc ggtgctgctg ccgccccatt
     2641 tgtagggcgt gccgatgagt ccgaggctgt ggagcatgag ttcctgcgag ccttgtgtgc
     2701 ggtcgatgtg gctgatgcgg acggcttgga tttgccggac tgtctgtttg ggtttcggtt
     2761 ggcggtgttt gccggaggtc gtgccgcatg aggcgaggag cagtgcgctg agacagagga
     2821 aaagggtttt gtcgggggga aacatggttt ttcctttgcg ggttcggata tccgtctgaa
     2881 ggcgtttcag acg
//
LOCUS       DAWHRS010000187         2863 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWHRS010000187 DAWHRS010000000
VERSION     DAWHRS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.187"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..328
                     /locus_tag="WIB82_001969"
     CDS             <1..328
                     /locus_tag="WIB82_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ3843216.1"
                     /translation="IATTVPAQQAFLDAAELMQWSIETEGLGLNVISHKILGKDHAQV
                     EFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGK
                     YLKPVA"
     gene            complement(547..1341)
                     /locus_tag="WIB82_001970"
     CDS             complement(547..1341)
                     /locus_tag="WIB82_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ3843217.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1445..2170
                     /locus_tag="WIB82_001971"
     CDS             1445..2170
                     /locus_tag="WIB82_001971"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ3843218.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISLGDRMDFTLQKSVELGIT
                     AIQPIISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYRKALDKM
                     PSESTKLIMSINRACKLADIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          779 a          865 c          635 g          584 t
ORIGIN      
        1 catcgctact accgttccgg cgcaacaggc attcctcgat gccgccgaac tgatgcaatg
       61 gagtatagaa accgaagggc tgggcttgaa cgtcatctcg cacaagatac tcggcaaaga
      121 ccacgcccaa gtcgaatttg aagcctactt ccgagacgga caacaccgat ccgcgcatca
      181 cgaactgtcc ggcttcgtca acatcggcgg acaatggtat tttatcgatc ccaccgttcc
      241 gcatcctgcg atgaaacaac cctgcatttg cggatcaggc aaaaaattca aagcctgctg
      301 cggcaaatat ctgaaacctg tcgcataaaa atgccgtctg aacgttcaga cggcattttc
      361 aacgtgcaaa aaaaaaccat tcataccaag ggtaagtatg aatggtcaat acattgcggg
      421 aaaacgtctt acttgctgca ctgccgaaaa gggagaaacg gcagcggtaa tcagcggaaa
      481 ggattgtacc cgaattaata ttaagaaacg ttaatcgcga aaatatatta acaaacctgt
      541 tgaaacctat tggttttccc gtatccaccc gacccagcgt tcaaacagct tcggttcgag
      601 cgcggcaacg accgagcgtt tgaacacgtg ttcaccactc caaaacccgt cgccttccaa
      661 agtcgtcagc ctgccgcccg cctcctcgaa aatcaacgcg ccggcggcat aatcccacag
      721 cttctgcccg ccgtgaacat aaacatcata acgcccgcac gccagataac accaatccaa
      781 cgtactgctg cccatactcc gtatcgttcc aaaaggcgcg agcgtactca tacggctgga
      841 aagtttgccc gaacgcagat atttgatttc cacgcccgca atcgcctcat tgagtttttt
      901 atccacgagg cgcaggggca gacgcgtccc gtttaaaaac gccccctgcc cgcgttcggc
      961 ataaaaacat tcgccgctga ctgggttgta gattacgccc aactcggcgc gcccgttgcg
     1021 gacaaacgcc accgataccg caaaatgcgg cagcccgttg acaaaattgt tcgtcccgtc
     1081 tatcggatcg acaatccaca gccccttttc ccccgaatat tgttcccaca aagccgactg
     1141 ttcctgccgc gacatttcct cacccaacat cggactgtcg attaaaagcg gcaacgcggc
     1201 ggcaaaagcc gtctgcgcgg caatgtccgc ctcgctcaac atcgaaccgt cttccttgcg
     1261 gtgagacggc gtattcaaaa aacgcggcat aatttcggtt tgcgcgatat ggcgcacgac
     1321 tttctgcaaa cggtgtaaca cttcctactg tcctcatatt ttgaacttgc ggcgcgcgaa
     1381 cgtataatgt ccgcttccat cacgccgctg cgacggatta taaccgtccg aaccgccaaa
     1441 aactatgccc cgattccacc tgcccgaaaa cctttccgtc ggacaaaccg tcgccctgcc
     1501 cgacaacatc gtccgccacc tcaacgtcct gcgcgtccgc cccaacgaaa acatcaccct
     1561 cttcgacggc aaaggcaagg cacacgccgc acggctgacc gttttggaaa aacgccgcgc
     1621 cgaagccgaa atcctgcacg aagacacaac cgacaacgag tccccgctca acatcacgct
     1681 catccaatcc atttccttag gcgatcgcat ggatttcacc ctgcaaaaaa gcgtcgaact
     1741 cggcataacc gccatacagc ccatcatcag cgaacgctgc atcgtccgcc tcgacggaga
     1801 acgcgccgcc aaacgcctcg cacgctggca ggaaatcgtc atctccgcgt gcgaacagag
     1861 cggcaggaac accgttcccc ccgtactgcc tatcatcggc taccgtaaag cactcgacaa
     1921 aatgccgtct gaaagcacca agctgattat gagcatcaac cgcgcctgca aacttgccga
     1981 catccgccac ccgtccggcg caatcgtctt tatggtcggc cccgaaggcg gctggacaga
     2041 acaggaagaa caacaggcat ttgaagctgg ctttcaggcg gttacactcg gcaaacggat
     2101 tttacgcaca gaaaccgccc cactcgccgc cctcgccgcc atgcagacgc tttggggcga
     2161 tttcgcataa acagaaatgc cgtctgaaac ccgttcagac ggcattttgc agccgattaa
     2221 gatagtaggt tcaaataaga tttcccgtgt cgtcattccc gcgaaagcgg gaatctagaa
     2281 acgaaaaact acagagattt atccgaaaca acaaccctct ccgccgtcat tcccgcaaaa
     2341 gcgggaatct agaaacgaaa aactacaggg atttatccga aacaacaaac cctctccgcc
     2401 gtcattcccg cgcaggcggg aatctagaaa cgaaaaacta cagggattta tccgaaacaa
     2461 caaaccctct ccgccgtcat tcccgcgcag gcgggaatct agaaatttaa cgttgcggtg
     2521 atttatcgga aatgactgaa actcaacgga ctggattccc gcctgcgcgg gaatgacgaa
     2581 ttttaggttg ctgttttttg gttttctgtt tttgcgggaa tgacgaattt taggtttctg
     2641 tttttggttt tctgttctcg cgggaataac ggaactttaa gttttaggaa tttgtcggaa
     2701 aaacagaaat ccccccgccg tcattcccgc aaaagcggga atctagaaac gaaaaactac
     2761 agggatttat ccgaaacaac aaactctctc cgacgcccac aaaagcggga atctagaaat
     2821 ttaacgttgc ggtgatttat cggaaatgac tgaaactcaa cgg
//
LOCUS       DAWHRS010000188         2862 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWHRS010000188 DAWHRS010000000
VERSION     DAWHRS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.188"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            348..1862
                     /gene="katA"
                     /locus_tag="WIB82_001972"
     CDS             348..1862
                     /gene="katA"
                     /locus_tag="WIB82_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ3843219.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            1938..2231
                     /locus_tag="WIB82_001973"
     CDS             1938..2231
                     /locus_tag="WIB82_001973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843220.1"
                     /translation="MPSETSFDRFRRHFHTPPPPFQWAFFIKRKISRHSHESGNQGLG
                     VGETVLSDKFPHRQLWIPACAGMTGFLFLIFCFCGNDGMRVFVRHFCIFLLLL"
     gene            2259..2825
                     /locus_tag="WIB82_001974"
     CDS             2259..2825
                     /locus_tag="WIB82_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ3843221.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWVAFVLLGGLGLKMMREGLSGEAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTTGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          703 a          775 c          755 g          629 t
ORIGIN      
        1 gataatttgg ggaaaattgt tattttcagc ctgtcggcgc aaaaaatgcc gcaaagcgtc
       61 attgcattat aaatggtttt aatgagcggg ttcggattcc gttcgataac aaaaaacaaa
      121 cgaaaatcaa aaaccgattg cttataataa tattaaatcg atttcatagt tttaatagcg
      181 aaaatcttgg cgtatagtcg catccatagt ttttacaaaa gggaaataaa atgtcgattc
      241 aagaaattta ttgcaatcaa gaaaccggtt acgaatacgc tttccgccaa atcgtactgt
      301 aagccgttta cccccatttg accaacctga gaaaaggaac aagagcgatg actacctcca
      361 aatgccctgt aacccatctg accatgaaca acggcgcgcc tgttgccgac aatcaaaaca
      421 gcctgaccgc cggtcctcgc ggccctctgc tggcgcagga tttgtggctg aatgaaaaac
      481 tcgccgactt cgtgcgcgaa gtcatccccg aacgccgtat gcacgccaaa ggttcgggcg
      541 cgttcggtac gtttaccgta acgcacgaca tcaccaaata cacccgcgcc aaaatcttca
      601 gcgaagtcgg caaaaaaacc gagatgttcg cccgtttcac caccgtggca ggcgaacgcg
      661 gcgcagccga tgcagaacgc gacatccgcg gttttgcctt gaaattttat accgaagaag
      721 gcaactggga tgtggtcggc aacaacacgc ccgtgttctt cctgcgcgac ccgcgtaagt
      781 tccccgacct gaacaaagcc gtcaaacgcg acccgcgcac caatatgcgc tctgccacaa
      841 acaactggga cttctggacg ctgctgcccg aagcactgca ccaagttacc atcgtgatga
      901 gcgaccgcgg catccccgcc ggctaccgcc atatgcacgg cttcggttcg catacctaca
      961 gcttctggaa cgaagcaggc gagcgttttt gggtgaaatt ccatttccgc acccaacaag
     1021 gcattaaaaa cctgaccaac gaagaagccg ccaaaatcat cgccgacgac cgcgaaagcc
     1081 atcagcgcga cttatacgaa gccatcgaac gcggcgagtt tccgaaatgg acgatgtaca
     1141 tccaagtcat gcctgaagca gacgcggaaa aagtacctta tcatccgttt gacttgacca
     1201 aagtttggcc gaaaaaagac tatccgctga ttgaagtggg cgaattcgag ttgaaccgca
     1261 atcccgaaaa cttcttcgcc gatgtggaac aatccgcctt cgcaccgagc aacctcgttc
     1321 ccggtgtcgg cgccagccca gataaaatgc tgcaagcgcg tttgttcaat tacgccgacg
     1381 cacaacgcta ccgtttgggc gtaaacttcc gccaaattcc cgtcaatcgt ccgcgttgcc
     1441 ctgttcacag caaccagcgc gacgggcaag gccgcgccga cggcaactac ggcagcctgc
     1501 cgcactacga acccaacagc ttcggccaat ggcagcaaca acccgacttc gccgaaccgc
     1561 ctttgaaaat caacggcgac gcggcacact gggactaccg ccaagacgat gacgactatt
     1621 tcagccaacc gcgcgccctg ttcaacctga tgaacgacgc gcagaaacag gcattgttcg
     1681 gcaacaccgc cgccgcaatg ggcgacgcgc ccgacttcat caaataccgc catatccgca
     1741 actgctaccg ttgcgacccg gcatacggcg aaggcgtggc aaaagccctc ggactgactg
     1801 tcgaagacgc acaagccgcc cgcgcgaccg accccgcact cggccagccc ggtttgctgt
     1861 aagggggcat tatgtggatg gaaattgaag aaatcctgtc ccgcgccgtc cgctaccgga
     1921 aataaccggg cataaaaatg ccgtctgaaa cgtcgtttga ccgcttcaga cggcatttcc
     1981 acaccccacc cccgccgttt cagtgggcgt tttttattaa acgcaaaata tcccgtcatt
     2041 cccacgaaag tgggaatcaa ggactcgggg ttggagaaac cgttttatcc gataagtttc
     2101 cgcaccgaca actctggatt cccgcctgcg cgggaatgac gggatttctg tttttgattt
     2161 tttgtttttg cgggaatgac gggatgcggg ttttcgtgcg gcatttttgc atttttttgc
     2221 ttttgctata atccgccctt tttgaggacg ggtgcggtat gggtttttat gctttgctct
     2281 tgattgctct ggggatgtcg atggatgcgt ttgccgtcgc attggcaaag ggtgcggcgg
     2341 tcagaatgcc tccgcgcaaa attgcggcaa cggctttggt gttcggcacg gttgaagcgc
     2401 tcacgccgct ggcaggctgg gtaggcggtt tttatgccaa gccgtttatc agcgaatggg
     2461 accattgggt ggctttcgtc ctgctgggcg ggctgggtct gaaaatgatg cgcgaagggc
     2521 tgtccggcga ggcggaagat gtgcgcgaaa gcaaacggga aagcctatgg atgacggttt
     2581 tgactgcttt tggaaccagt attgattcca tgatagtcgg ggtgggcttg gcgtttatgg
     2641 aggtaaacat cgcctttgcc gccgcaatca tcggtatggc gacgacggtg atggtggcgg
     2701 tcgggctgac gacgggaagg gctttgggcg tattgttcgg caggtgtgcg gaatttgccg
     2761 gaggtttggt gttgattgcc atcggcacat ggacgctctt gtcgcatttg ggtttgattc
     2821 aatgatgtcg gaaaatatag tggattaaca aaaaccagta cg
//
LOCUS       DAWHRS010000189         2775 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWHRS010000189 DAWHRS010000000
VERSION     DAWHRS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.189"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(66..2231)
                     /locus_tag="WIB82_001975"
                     /pseudo
     CDS             complement(66..2231)
                     /locus_tag="WIB82_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT          589 a          636 c          791 g          759 t
ORIGIN      
        1 atcaaattat caggacagat gccgtctgaa aggctttcag acggcatttt ttcgggatgt
       61 gcgttttaga acttgtagtt cacgcccaag cgtacatcac gtcccacgcc cggcagggta
      121 ttggtccagc gttggctgtg cggatagtag aacgtgttga acacgttgtt aaccgaaaga
      181 ttaacattga gcgtgtcttt gcccagcggt ttccagttgg cgaagacatc gttcacaccg
      241 aaacctttgc gtacaacgtt ttccaatttg ccgttgcggt ctttttgacc tgccaccaat
      301 atcgaaccca cggctttttg aacataacgg ccgcgccagc cgatttccag attcgggttt
      361 ttgaagcggt aggcaaggga ggctgtccaa gtgcggccga cttgtgcgcc aaattcagga
      421 ttcgcgctca acagcttgtc tttgtgcgta tcgtaaaagc gcggtttgct gtggcttacg
      481 ccgactttgg cagtcaggcc gccggtgcgg taggacgcgc ccaattcgta accgtggttt
      541 ttgatgtaac cggcattgac ggcttcacgg acagcggcag agacgtggcg gttttgcgga
      601 ttggcaagcg cgtctttgat ggtttgccag aagtagctgc cgttggcggc aaacgtgccg
      661 tcgttgtagt tgaagccgat ttcggtattg cgcgcgcgtt cggctttcgt gccgtcggca
      721 atcgagatga tgccgcgttt accgtgggtt tgcagcgcgt catacaggcg cgggctgcgg
      781 ctggcgtagt tgtggctcgc gctgaagctc cagtgttcgt gcggctgcca aatcacaccg
      841 aaactcgggt taaggttgct gcttgaaacg gttttgccgt catgggtttt caccttgaag
      901 cggtcgtaac gcagcccgcc ggtcagggta aagccgtcaa tctcgtgaat ggcttcgata
      961 tacgcgccgg tatcggtttt ggtcgggtta gacagtttgt aggcgtgaac aattttttca
     1021 ttggcacgat ttttttcttt ttgtgcatca tctacgtcga cttcttttcc gtcggctcct
     1081 tttttcttat cggagatctc aaatttcgag ttcaaaaacg cttgcggttt gatttcctga
     1141 tggcggtagt tgataccgta tttcagcagg gtttgttcgg caaggcggct gtcgaagttg
     1201 aagttcgcac cacgagtggt gattcgggta tggttggggc cttttacatt gcctgcgtag
     1261 ccggtgccgc tgtcatcggc ggaatagcgt tctttttcca acacataggc gttggcatcc
     1321 agtttttcga caaagcccag gtttttaccc gtgtacgcca aattggtgtt ggattgtgtg
     1381 gtttcgcggt aagcaggggc ttggcgttta atatttatcc gtgaatcttc gctgccgacg
     1441 gtaaattctt cacgcacagt gcggatgccc cggtgttggt ctttcatatg gctcaacacg
     1501 atgcggtggt cgccgtcgcc gaaggttgtt ccgattttgg cgaggtagct gcgtttgtcc
     1561 agcgcgctgt acggtacggt tttgccgccg ttgacattgc ggaaaccttt gccggcttcg
     1621 taatcttttt catcgttgcg gttgtaagag aacaagccgt cgaagttgcc ctcttttccg
     1681 aatacgctgg cgccgtagct tacgccttcg ttgctggcaa agccgctgtt gaggcgcacg
     1741 ccctagtttt tatccaagcc tttgagcagg tcttgggcat cgacggtttt ggcgataatc
     1801 gcgccgttgg tcgcgccgat accggcagag gcggaacccg cgcctttttg tacggaaacg
     1861 actttaacca aagcgggatc gacaataaat ctgccttggt ggtaaaggat ttggctgtcg
     1921 gaataggcgt tgtccacctt gatgtcgaca gagttctgac ccatgccgcg cagcgtcagg
     1981 aattgggacg tgccgttgcc gccgccgaaa tcgatggaag gctcttcttt taagagttcg
     2041 cgcatatcgg ttgcggtgct ttcgtctttt tgttgcagcg taacgatgtt ggtacggatt
     2101 ttgctgcctt ggcggtcgcc ttttacggta acggtatcca gtacgacctt ggcattattt
     2161 tctgccgcgt gggcaaaacc tgccgccagg gtaagcgaga gcaggctgag gcggaataat
     2221 ggggcgttca tttgttcgtc cttttgagtg tatgaaggga agtaaatcca aaccgttaag
     2281 gtttggcagg ataagaaaaa taataattat ttttgtttat attaacgaga ggggggattt
     2341 gtgtaaagct gattatcgtt tttatttgcg aagtgttgtt ttttgttgac aggttttgtc
     2401 ggaaatgtaa aaacggcgga aatatagtgg attaacaaat gcgggaatga cgaaaagcaa
     2461 cctttccgcg tcattcccac gaaagcggga atctagaacc cctaacgcgg caggaatcta
     2521 tcggaaatga ctgaaaccga acgaacctag attcccgcct gcgcgggaat gacgaagcct
     2581 gcgcgggaat gacgaaaagc aacctttccg cgtccttccc gcaaaagtgg gaatctagga
     2641 actcaaagct gcaagaattt atcagaaaca actggaactc aaaaaaacta gattcccgcc
     2701 tgcgcgggaa tgacgaaggg ttaaagaaat gacgagaaac ggcggtaatt tatcgggaat
     2761 aaccgaaaac gaacg
//
LOCUS       DAWHRS010000190         2759 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWHRS010000190 DAWHRS010000000
VERSION     DAWHRS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.190"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            79..1095
                     /locus_tag="WIB82_001976"
     CDS             79..1095
                     /locus_tag="WIB82_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3843222.1"
                     /translation="MYDFLHHRPYLQCGKYLRCCVDSVLAENFADYEMILVDDGSPDG
                     CGKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKN
                     ARGGILFDLQQLADKKVDLILHPSSFNYRDIPKGADFSDNDFVRHFETLVEGRYYIAN
                     AWTKIVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSIS
                     HNIKYKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSISVRLYFSKNI
                     IVVYREYFSFKEKCRKIFGAKAIRPIFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQK
                     FFSE"
     gene            1311..2648
                     /gene="ribB"
                     /locus_tag="WIB82_001977"
     CDS             1311..2648
                     /gene="ribB"
                     /locus_tag="WIB82_001977"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ3843223.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADEETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
BASE COUNT          740 a          783 c          631 g          605 t
ORIGIN      
        1 ttacagggtg cggatgcaaa cgcattgcga gcgcgggttt gaggcatacg cgcaaacatc
       61 ttaatataat ggattgatat ttatgatttt ctccatcatc gtccctattt acaatgtgga
      121 aaatacctcc gctgctgcgt ggattccgtg cttgccgaaa attttgccga ttatgaaatg
      181 attttggtcg atgacggttc gccggacggc tgcgggaaga tttgcgacga atatgcaggc
      241 aaatatccgc atataaaagt gattcatcaa gaaaacggcg ggctgtcgga tgcccgcaac
      301 gccggtatcc gggcggcaaa aggcgattac ctaatctttt tggacagcga cgactattgg
      361 gccgacacca atcgttcaaa aaacgcgcgg ggggggattc tctttgattt acaacaactt
      421 gcagacaaaa aggttgattt gatcctgcat ccctcgtcct tcaattaccg cgacatcccc
      481 aaaggggcgg acttttcgga taatgatttt gtccgccatt ttgaaacgct ggtggagggg
      541 cggtactata tcgccaacgc gtggacaaaa atcgtcaggc gggaaataat catcaaaaac
      601 aatctgtttt tcccaaaagg atacattcac gaggatttcc cgtacagttt gcaattggcg
      661 cgttttatca agacttttgc cttttacgat aacccttttt accagtaccg cgttctcggc
      721 ggctctatca gccacaacat caaatacaaa aatttcagcg atgtgctgac gcatctcgac
      781 cggggtgtgg attttttagt cgaaaacaaa aattccccca tctacggcgg tttgcaaaaa
      841 tttgtcttcg acaatatcgg ctatctgaga tctatatcgg taaggcttta tttttccaaa
      901 aacattatcg ttgtctaccg agaatatttt tcatttaaag agaaatgcag aaagatattc
      961 ggcgcgaagg caatccgtcc gatttttatc gggaagaccg cattcatcat aggattgccg
     1021 atattgcgcc tgctcgtacc gcctatgctg tacccggcaa tcaaggccgt ttatcagaaa
     1081 tttttttcgg aataagcttc cggcaaaccc cgaatcggaa gcgggcggga agaaacagcc
     1141 gccccgccgg cggggattgc ggcaatgccg tctgaagcca cgaatccggc ttcagacggc
     1201 atctgtttac caaaaagcaa ataattcggt ttggcgaaaa aaacagattt gctttttggt
     1261 aaatacgcga ttacaatccg ctacatccga tttctacaaa ggatgaaacg atgaccgaca
     1321 cagctggtct gcgccgccgc aacctgcggc aatggatagc agaacactac ggcggtttgc
     1381 aaacccgttt tgccgaagcc gttgccctca acacaggcga actctccgcc cttttgaaaa
     1441 acaaatcctt cggcgagaaa aaagcccgta aaatcgaaca ggcggcaaaa atgcccgcct
     1501 tctggctcga taccgaacac accgcccgcc cgcccgaaca cacaggaaaa cacaccatgt
     1561 cccatatttc ccccatcccc gaaatcctag ccgacatcaa agccggcaaa atggtcatca
     1621 tcaccgatgc cgaagaccga gaaaacgaag gcgacctgct gatggcggcg caattcgtca
     1681 cgcccgaagc catcaacttc atgatcaaac acgcgcgcgg cttggtctgc ctgccgatgg
     1741 acggcgaaat ggtcgaaaaa ctcgggctgc cgatgatgac ccaaaaaaac ggcgcgcaat
     1801 acggcaccaa ctttaccgtc tccatcgaag ccgcacacgg cattaccacc ggcatttccg
     1861 ccgccgaccg cgccctgact attcaaaccg ccgtttcccc gaccgccaaa cccgaagaca
     1921 tcgtccaacc cggtcatatc tttccgcttc gcgcccaaaa aggcggcgta ctcgtccgcg
     1981 ccggacacac cgaagccggc gtcgacctgg cgcaaatgaa cgggctgatt cctgccgccg
     2041 ttatttgcga aatcatcaac gacgacggca cgatggcgcg tatgcccgaa ctgatgaaat
     2101 tcgccgaaga acacaagctc aaaatcggca ccattaccga cctcatcgaa taccgcagcc
     2161 gtaccgaaag cctgcttgaa gacatgggca acgcacccgt acaaaccccg tggggcgagt
     2221 tccaacaaca cgtttacgtc gacaagctct ccggcgaaac ccacctcgcc ctcgtcaaag
     2281 gtacaccttc cgccgacgaa gaaaccctcg tccgcgtcca cgaacccttc agcgtcatgg
     2341 acttcatcca agccaacccg cgtcattcat ggtcgctgcc caaagcactc gagcgcatcc
     2401 aacaggccga aagcggcgta gtcatccttc tgcaccgcac cgaagacggc gcatccctgc
     2461 tcgaccgcac cctacccaaa ggcgcaaacc aagcctacaa atgggacagc aaaagctacg
     2521 gcatcggcgc acaaatcctc gtcggcctca acgtcaaaaa actgcgcgtc ctcgggcagc
     2581 cctcatcttt taccggcctg accggcttcg gtttggaagt cgtcggcttt gaagaagcgg
     2641 aaaaataata tagtaaattc aaatacttta tattttcttt atttattgca ttatttccgt
     2701 gcaaacgaaa acccggtctg ttgggttgga ttttgttttt gcaaatttcg ggtaacttc
//
LOCUS       DAWHRS010000191         2691 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWHRS010000191 DAWHRS010000000
VERSION     DAWHRS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.191"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            45..1940
                     /gene="mnmG"
                     /locus_tag="WIB82_001978"
     CDS             45..1940
                     /gene="mnmG"
                     /locus_tag="WIB82_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ3843224.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAATRMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLEHYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVSEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEGEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            2009..2593
                     /gene="rnhB"
                     /locus_tag="WIB82_001979"
     CDS             2009..2593
                     /gene="rnhB"
                     /locus_tag="WIB82_001979"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ3843225.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVEWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQALF"
BASE COUNT          723 a          874 c          664 g          430 t
ORIGIN      
        1 atccactata aatgccgtct gaaacaccga caggcaatac acacatgacc cacatgattt
       61 accccaaaac ctacgacgtt atcgtcgtcg gcggcggaca cgccggcacg gaagccgcgc
      121 ttgccgccac acgcatgggc gcgcagacgc ttttgctcac acacaatatt gaaacgctcg
      181 gacaaatgtc gtgcaacccc tccatcggcg gcatcggcaa agggcatttg gtacgcgaac
      241 tcgacgcgct cggtggcgca atggcgttgg caaccgacaa atcaggtatc caattccgcc
      301 gcctgaacgc cagcaaaggc gcggcagtgc gagccacgcg cgcacaggcc gaccgcattt
      361 tgtataaagc cgccatccgc gaaatgttgg aaaaccaaga aaacctcgac cttttccaac
      421 aagccgtcga agacgtaacg ctcgacggcg aacgcatcag cggcgtaatt accgcgatgg
      481 gcgtggagtt taaagcacgc gccgtcgtgt tgaccgcagg cacgtttttg tccggcaaaa
      541 tccacatcgg tttggaacac tacgaaggcg gacgcgccgg cgaccccgcc gccaaatcgc
      601 tgggcggacg tttgcgcgaa ttgaagctgc cgcaaggccg tctgaaaacc ggcacgccgc
      661 cgcgcatcga cggacgcacg attgacttct cccaactgac cgaacagccc ggcgacacgc
      721 ccgttcccgt catgtccgta cgcggcaacg ccgaaatgca cccgcgccaa gtgtcctgct
      781 ggattacgca taccaacacg caaacccacg acatcatccg ctcaggcttc gaccgcagcc
      841 cgatgtttac cggcaaaatc gaaggcgtgg gcccgcgtta ttgtccgtct atcgaagaca
      901 aaatcaaccg cttcgccgac aaagacagcc accagatttt cctcgaaccc gaaggcctga
      961 ccacgcacga atactacccc aacggtatct ccaccagcct gccgttcgac atccaaatcg
     1021 cgctcgtccg cagtatgaaa ggtttggaaa acgcccatat cctgcgcccc ggctacgcca
     1081 tcgaatacga ctacttcgat ccgcgcaacc tcaaagcaag cctcgaaacc aaaaccattg
     1141 ccggattgtt tttcgccggg caaatcaacg gtacgacagg ttatgaagaa gccgccgcgc
     1201 aaggactgct ggcaggcgcg aacgccgtgc aatacgtccg cgaacaagac ccgctcctgc
     1261 tgcgccgcga acaagcctat ctcggcgtat tggtggacga cctcatcacc aaaggcgtga
     1321 acgaacccta ccgaatgttc accagccgtg ccgaataccg cctgcaactc agggaagaca
     1381 acgccgacat gcgcctgacc gaagacggct acaaaatcgg cttggtgtcc gaagcgcaat
     1441 ggcgcatgtt caacgaaaaa cgcgaagccg tcgaacgcga aatccaacgt ttgaaaacaa
     1501 cgtggtacac gccgcaaaaa ctcgccgaag gcgaacaaat ccgtgtgttc ggacaaaaac
     1561 tcagccgcga agccaacctg cacgacctcc tgcgccgccc aaacctcgac tacgccgcgc
     1621 tgatgacgct cgaaggcgcg atgccgtctg aaaacctctc cgccgaagtc atcgaacaag
     1681 tcgaaatcca agtcaaatac caaggctata tcgaccgcca aaacgaagaa atcgacagcc
     1741 gccgcgacat cgaaacctta aaactgcccg acggcatcga ttacggcaaa gtcaaaggct
     1801 tgtcggcaga agtgcagcaa aagctcaacc agcacaaacc cgaaaccgtc ggacaagcca
     1861 gccgcatttc cggcgtaacc cctgcggcag tggcattgct gatggtgcat ttgaagcgcg
     1921 ggtttaaaga cgcgaaataa acacatcggc gcgatgccgt ctgaaaccct tttcagacgg
     1981 cattcccacc atcccgacag gaaacatcat gcacatactg accgccggcg tggacgaggc
     2041 aggacgcgga cctttagtcg gcagcgtgtt tgccgccgcc gtcatccttc cggaaacatt
     2101 cgacctgccc ggactgaccg actccaaaaa actcagcgag aaaaaacgcg acgcgcttgc
     2161 cgaaatgata aaaaatcagg cggttgagtg gcacgttgcc gccgcctcgc ccgaagaaat
     2221 cgccagcctc aacatcctgc acgccaccat gctcgcgatg aaacgcgccg ttgacggctt
     2281 ggctgtgcgt cccgaaaaaa tattcatcga cggcaaccgc attcctgaac atttgaacat
     2341 ccctgccgaa gccgtcgtca aaggcgacag caaaatcatc gaaatctccg ccgcatccgt
     2401 tttggcaaag accgcacgcg atgcggaaat gtacgcactg gcgcaacgcc atccccaata
     2461 cggtttcgac aaacacaaag gttacggcac gaagcagcat ttggaagccc tcgaaaaata
     2521 cggcgtgctg cccgaacacc gccgcgactt cgcccccgtc agaaacctgc tcgcgcagca
     2581 ggccttgttt taaaccggca caaaaatgcc gtctgaagcc ttcagacggc atttccggca
     2641 tcgcaactcc aaagagaaag aacccaaacc gtcattcccg cgaaaaatag a
//
LOCUS       DAWHRS010000192         2638 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWHRS010000192 DAWHRS010000000
VERSION     DAWHRS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.192"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            315..2540
                     /locus_tag="WIB82_001980"
     CDS             315..2540
                     /locus_tag="WIB82_001980"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ3843226.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFTRHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT          732 a          800 c          612 g          494 t
ORIGIN      
        1 aatcaggaca aggcgacgaa gccgcagaca gtacaagcag tacgacaagg cgaggcaacg
       61 ctgtactggt ttaaatttaa accactataa aatcaatttc accgaaaata tgccgccgca
      121 catcaggcac agcacctccg accgccacca aacgcgggaa aaccatttcc cccttccttt
      181 cacgctgccg cgttcaaaca cgccaagatc gaacaaagcg cgaacaaagc cgccaaaata
      241 tgccataata gccgcgcaca ttccccgcgc aatgtgttcc aaaagaaaaa accaacctac
      301 aagagaataa tcctatgact caaaaatcca ccattgttta tacccatacc gacgaagccc
      361 ccgcgctggc gacccaatcg ctgctgccga ttgtgcaggc ttttacccgc cacgccgata
      421 ttgatgtcaa aactgccgac atttccctgt ccggccgtat tttggcggca ttccccgaat
      481 atctgaccga agcgcaacgc gtacccgacg cgcttgccga attgggcgaa ctggtaaaac
      541 aacccgatgc aaacgtaatc aaactgccga acatcagcgc atccgtaccg caactgaccg
      601 ccgcgattaa agaattgcag tctaaaggct ttgccgttcc cgattatcct gccgacccgc
      661 aaaccgatga agaaaaagcc gtgcgcgaac gctacgaccg catcaaaggc agcgcggtaa
      721 accctgtcct gcgtgaaggc aactccgacc gtcgcgcccc caaagcggtg aaaaactttg
      781 ccaaaaaaaa tccgcacagc atgggcgcat ggacaaaaga ctccaaaacc cacgttgcca
      841 ccatgcaaag cggcgacttt ttccataacg aacaatccgt taccgtacct gaagcgactt
      901 ccgtatccat cgtgttcacc gacaaacaag gcaataaaaa agagctgcgc gcgcctgttg
      961 ccctgaaagc cggcgaaatc atcgacgcga ccgtgatgag taaaaaagcc ctgctcgcct
     1021 tccttgccga acaagtaaaa gatgcgaaag caaaaggcgt gttgttctcg ctgcacatga
     1081 aagccactat gatgaaagtg tccgacccga ttatcttcgg acacgccgtc aaagtattct
     1141 tcgcgcctgt ttttgaaaaa ttcggcgaca aactggctgc tgccggcgtc aacgttaaca
     1201 acggcttcgg caacctgctt gccaatctgg acaaactgga tgcggacacc cgcaccgccg
     1261 tcgaagccga aatcgccgcc gtttacgccg ccaaccccga tttggcgatg gtcgattccg
     1321 ataaaggcat taccaacctg cacgttccca gcgatgtcat cgttgatgct tctatgcctg
     1381 cgatgattcg taactccggc cgtatgtggg ataaagacgg caaagcgcaa gacaccaaag
     1441 ccgtgattcc cgacagcagc tacgccggcg tttaccaagc aaccatcgac ttctgccgcg
     1501 aacacggcgc atttgacccg acaaccatgg gtactgtgcc caacgtcgga ctgatggcgc
     1561 aagcggcgga agaatacggc tcgcacaaca aaacctttga aatcgaagcc gacggccaag
     1621 tccaagtcat tgatgcggca ggaaaagtcc taatgcagca cgacgttgaa gccggcgaca
     1681 tctggcgtat gtgccaaacc aaagacgctc cggttaaaga ctgggtacaa cttgccgtca
     1741 accgcgcccg tctgagcaac acgcctgccg tgttctggct cgacgaaaac cgtccgcacg
     1801 acaagagcct gctcgccaag gttaaagcct accttgccga actggatacc aatggcctcg
     1861 acatccgcgt cctcgctcct gaagaagccg ccaagttcag cttgggtcgt ctgaaaaacg
     1921 gcgaagacac catctccgta accggtaatg tcttgcgcga ctacctgacc gacttgttcc
     1981 caattttgga actcggcaca agcgcgaaaa tgctgtctat cgttccattg atgaacggcg
     2041 gcggtatgtt tgaaaccggc gcgggcggtt ctgcaccgaa acatgttcaa caattcctcg
     2101 aagaaaacca tttgcgctgg gactcgctgg gcgaattcct cgcactcgcc gtatcgtttg
     2161 aacatttggc gcaaaaaacc ggcaatgcca aagcccaagt cctcgccgac actttggatg
     2221 cagccaccga aaaactgctg ttgaacgaca aatcgcctaa acgtaaagcc ggcgaactcg
     2281 acaaccgcgg cagccatttc tacctcaccc tctactgggc gcaagaattg gcggcgcaag
     2341 acaaagatgc cgaactgaaa gccgcattta cgccattggc agccgctttg accgccgacg
     2401 aagcgaaaat cgttgccgag ctttccgccg tacaaggcaa agcggccgac atcggcggct
     2461 actacgccgc caatcctgaa aaagccgcac aagtgatgcg tccgagcgta acctttaatc
     2521 aggcactaaa cgccttataa gcacaaaggt aaaaatgccg tctgaacatt cgtcgttcag
     2581 acggcattag ttatccctat ctgcctgatt atgattaaac gccgcaccca ttggatat
//
LOCUS       DAWHRS010000193         2628 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWHRS010000193 DAWHRS010000000
VERSION     DAWHRS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.193"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            121..735
                     /locus_tag="WIB82_001981"
     CDS             121..735
                     /locus_tag="WIB82_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ3843227.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVLG"
     gene            896..2557
                     /locus_tag="WIB82_001982"
     CDS             896..2557
                     /locus_tag="WIB82_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ3843228.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPDE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADSYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDYHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          515 a          664 c          682 g          767 t
ORIGIN      
        1 aaggcgaggc aacgccgtac cggtttttgt taatccacta tattttgacg gggttgaagt
       61 tgacatctac aaggtttaat gtttgaatac attcaaaaat atatttgaat gagatgtttt
      121 atgattcaaa atgtcgttac ttcaataatc ctgtattctg ggacagccgt agacttactt
      181 attatcctaa tgttattttt tgccaaaaga aaaagcagaa aagacatcat taacatctat
      241 ttaggacaat ttctaggctc tgttagcctg atattgctaa gtttgctttt tgcatttgtc
      301 ttagattata ttcctagtaa agagatttta ggtttgctcg gcttgattcc aattttccta
      361 ggcatcaaag ttttgctgtt aggagattct gatggagagg ctattgccaa agagggtttg
      421 cgcaaagata ataaaaacct gatttttcta gtcgctatga ttacttttgc aagttgtggt
      481 gctgacaata ttggtgtctt tgtcccatat tttactacct taaattcagc gaatttgata
      541 gtggctttac ttacctttct agtcatgatt tatctcttgg ttttttctgc ccaaaaattg
      601 gcacaagtcc cttctgtcgg agaaactttg gaaaaatata gcagatggtt tgttgctgtt
      661 gtttatttag gattgggggt atatatcctg attgaaaaca acagttttga tatgctatgg
      721 actgtgttgg gctaggaaaa aatattatga aaaagatagt atctgcaaag actgccatgt
      781 gcagtctttt ttgttgccgg tctttttgtg tctgatgccg tctgaagcag tctctgcacg
      841 acctttgtgc gaatatttgc tacacttggc aatccgtcca ttttttctca cgcttatgct
      901 gacctatacc ccgcccgatg cccgcccgcc cgccaaaacc cacgaaaagc cgtggctgct
      961 gctgttgatg gcgtttgcct ggttgtggcc cggcgtgttt tcccacgatt tgtggaatcc
     1021 tgacgaacct gccgtctata ccgccgtcga agcactggca ggcagcccca cccccttggt
     1081 tgcccatctg ttcggtcaaa ccgatttcgg cataccgccc gtgtatcttt gggttgccgc
     1141 cgcgttcaaa catttgctgt cgccgtgggc tgccgactca tacgatgccg cacgctttgc
     1201 aggcgtattt tttgccgtta tcggactgac ttcctgcggc tttgccggtt tcaacttttt
     1261 gggcagacac cacgggcgca gcgtcgtcct gattctcatc ggctgtatcg ggctgattcc
     1321 agttgcccat ttcctcaacc ccgctgccgc cgcctttgcc gccgccggac tggtgctgca
     1381 cggttattct ttggctcgcc ggcgcgtgat tgccgcctct tttctgctcg gtacgggctg
     1441 gacgctgatg tcgttggcag cagcttatcc ggcagcattt gccctgatgc tgcccttgcc
     1501 cgtactgatg tttttccgtc cgtggcaaag caggcgtttg atgttgacgg cagtcgcctc
     1561 acttgccttt gccctgccgc ttatgaccgt ttacccgctg ctcttggcaa aaacgcagcc
     1621 cgcgctgttc gcgcaatggc tcgactatca cgttttcggt acgttcggcg gcgtgcggca
     1681 cgttcagacg gcattcagtt tgttttacta tctgaaaaac ctgctttggt ttgcattgcc
     1741 cgcgctgccg ctggcggttt ggacggtttg ccgcacgcgc ctgttttcga ccgactgggg
     1801 gattttgggc gtcgtctgga tgcttgccgt tttggtgctg cttgccgtca atccgcagcg
     1861 ttttcaggat aacctcgtct ggctgcttcc gccgcttgcc ctgttcggcg cggcgcaact
     1921 ggacagcctg aggcgcggcg cggcggcgtt tgtcaactgg ttcggcatta tggcgttcgg
     1981 actgtttgcc gtgttcctgt ggacgggctt tttcgccatg aattacggct ggcccgccaa
     2041 gcttgccgaa cgcgccgcct atttcagccc gtattatgtt cctgatatcg atcccattcc
     2101 gatggcggtt gccgtactgt tcacaccctt gtggctgtgg gcgattaccc ggaaaaacat
     2161 acgcggcagg caggcggtta ccaactgggc ggcaggcgtt accctgacct gggctttgct
     2221 gatgacgctg ttcctgccgt ggctggacgc ggcgaaaagc cacgcgccgg tcgtccggag
     2281 tatggaggca tcgctttccc cggaattgaa acgggagctt tcagacggca tcgagtgtat
     2341 cggcataggc ggcggcgacc tgcacacgcg gattgtttgg acgcagtacg gcacattgcc
     2401 gcaccgcgtc ggcgatgtac aatgccgcta ccgcatcgtc ctcctgcccc aaaatgcgga
     2461 tgcgccgcaa ggctggcaga cggtttggca gggtgcgcgt ccgcgcaaca aagacagtaa
     2521 gttcgcactg atacggaaaa tcggggaaaa tatataaaaa acaacagatt gagccgaatt
     2581 tctggattaa gtgccggaaa tcgcgtataa ttgcgcgatt aaaccttt
//
LOCUS       DAWHRS010000194         2623 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWHRS010000194 DAWHRS010000000
VERSION     DAWHRS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.194"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..408
                     /locus_tag="WIB82_001983"
     CDS             <1..408
                     /locus_tag="WIB82_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEZ3843229.1"
                     /translation="SAMSARLARFVPEEEERRRLLVNIQYESSRAGLDTQIVLGLIEV
                     ESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRYGCTILRHYRNLEKG
                     NIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            519..1901
                     /locus_tag="WIB82_001984"
     CDS             519..1901
                     /locus_tag="WIB82_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3843230.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            1905..2429
                     /locus_tag="WIB82_001985"
     CDS             1905..2429
                     /locus_tag="WIB82_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ3843231.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          533 a          713 c          784 g          593 t
ORIGIN      
        1 tctgccatgt cggcacgttt ggcaaggttc gtccccgagg aggaggagcg gcgcaggctg
       61 ctggtcaata tccagtacga aagcagccgg gccggtttgg atacgcagat tgtgttgggg
      121 ctgattgagg tggaaagcgc gttccgccag tatgcaatca gcggtgtcgg cgcgcgcggc
      181 ctgatgcagg ttatgccgtt ttggaaaaac tacatcggca aaccggcgca caacctgttc
      241 gacatccgca ccaacctgcg ttacggctgt accatcctgc gccattaccg gaatcttgaa
      301 aaaggcaaca tcgtccgcgc gcttgcccgc tttaacggca gcttgggcag caataaatat
      361 ccgaacgccg ttttgggcgc gtggcgcaac cgctggcagt ggcgttgatt ttgaacccgc
      421 gccgcaaccg aaatacggcg aatcctgtat aatccgaaaa tctgttcact ggaagttcag
      481 acggcattgc aactgttgat gccgtctgaa aaaatatgat ggcaagagac aaccgcatcc
      541 aaatgtttcc gcacgaatgg cgcgccagta cgacgctttc cggcgtgtac gcgctgcgta
      601 tgctgggtat gttcctcgtg ctgcccgtat tggcggtgta tgccgcctcg ctgcccggcg
      661 cggaaggcaa caaaacgctg gtcgggctgg caatgggcat ttacgggctg acacaggctc
      721 tgctgcaact gcctttgggc atcgcttccg acaagttcgg gcgcaagaaa accatttatg
      781 cgggactggt cgtgtttgcg gcgggcagct ttcttgccgc cgccgccgat acgctgccca
      841 tgctggtcgc cgcacgcgcc atacagggtg caggggcggt cagtgcggcg gttaccgcgc
      901 tgctggcgga tttgacgcgc gacggcgtac gtacccgcgc aatggcgatg atcggtttga
      961 gtatcggttt gacgttttcg gtcagcctcg tcgttgcccc cgtgattgcc gacgcggtcg
     1021 gcgttcgcgg actgtttatg ctgaccggca ttctgaccgt catcagcatc ggcgtggtgg
     1081 cgtggatgac tcccgatccc gaagtttcca agctgcacga agatacgcag gcgcagcctt
     1141 cgcgcatagg cgaagttttg aaaaaccgta ggctgctgac gcttgatttc ggcattttcg
     1201 ccctgcacgc cgcacaaatg gcattgttta ccgcgctgcc tttcgcgatg acccagctcg
     1261 gtttggaaaa aatacagcat tggaaagtct atctgccttc gaccattacg ggcttggtgg
     1321 tgatggttcc gctgattatc gtcggcgaga cgcgcaacaa gcttaagcag gtttttgttt
     1381 tgggtatcgt ctgtattgcg gcggcgcagc tcggtttgct gtccggtatg cgctcggtag
     1441 gcttgattac cgcttatttg gttgtttact ttatcggttt taatgtgttg gaagcgagcc
     1501 tgccgtctat ggtttccaaa atcgcgccgt ccgacctgaa gggtacggcg atgggcgtgt
     1561 acaacacgat gcagtcgctc ggactgtttg ccggcggcgc ggcaggcggt ttgctgtttc
     1621 aaaaatacgg cttttccggc gtgtttgcct tttgcagtat attgatgctg ctgtggctgg
     1681 taattgccgt tttatcgcct gcgcccaagc ccgtcaaaaa cctcagttac cctgtcggcg
     1741 gcgtgtggca gggcaatcag gaagggttat accgcgcctt gtcggagctt gagggtgtgg
     1801 aagacatcgg tttcagtttc gacgggcaga ccgtctatct caaagtgttg cagaagggtt
     1861 tcgatcaggc tgccgctgaa aaaatcatca caggagttta aaaaatgtca ttgaacaaag
     1921 tcatcctcat cggccgcctc ggacgcgatc ccgaagtgcg ctatatgccc aacggcgagg
     1981 cggtttgtaa tttcagcgtc gccaccagcg aaacttggaa cgaccgcaac ggccaacgtg
     2041 tagagcgtac tgagtggcac aacatcacca tgtaccgcaa actggcggaa attgccgggc
     2101 aatacctcaa aaaaggcggg ctggtttatt tggaaggcag aatccaaagc cgcaaatacc
     2161 aaggcaaaga cggcatcgaa cgcaccgctt acgatattgt cgccaacgaa atgaaaatgt
     2221 tgggcgggcg caatgaaaac agcggcggtg cgccttacga ggaaggttac ggtcagagtc
     2281 aggaggctta ccaacgcccc gcgcagcaaa gccggcagcc cgcctccgac gcgccgtccc
     2341 atccccaaga agcaccagcc gcgccgcgcc gccaacccgt gcctgccgcc gccccggtcg
     2401 aggacattga cgacgatatc ccgttctgaa ttttacggcc ggacatcctc gcggagggaa
     2461 atcataaagg acggagaaac cttaaacctt acggggcagg gtttctcttt tttgcatttg
     2521 ggccggcttg ttgccgccct ttgcgttggg cggaacgttg cgattgcgcc gttcgggcgt
     2581 tttgaggcag tgatgcctgc cgcacgtccc cgctgttttc aga
//
LOCUS       DAWHRS010000195         2577 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWHRS010000195 DAWHRS010000000
VERSION     DAWHRS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.195"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..260)
                     /gene="rnhA"
                     /locus_tag="WIB82_001986"
     CDS             complement(<1..260)
                     /gene="rnhA"
                     /locus_tag="WIB82_001986"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="An endonuclease that specifically degrades the RNA
                     strand of RNA-DNA hybrids; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ3843232.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKR"
     gene            374..1243
                     /gene="tehB"
                     /locus_tag="WIB82_001987"
     CDS             374..1243
                     /gene="tehB"
                     /locus_tag="WIB82_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ3843233.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSMYCTAADYFH
                     KKYGMSATHSAVVAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGSMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(1293..2099)
                     /gene="thiD"
                     /locus_tag="WIB82_001988"
     CDS             complement(1293..2099)
                     /gene="thiD"
                     /locus_tag="WIB82_001988"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ3843234.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            2160..2357
                     /locus_tag="WIB82_001989"
     CDS             2160..2357
                     /locus_tag="WIB82_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843235.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
BASE COUNT          566 a          624 c          759 g          628 t
ORIGIN      
        1 cgcttccaac cgtgtatcca gttttccatg ccatttttga cgtattgcga gtcggtgcag
       61 atgatgacgg tgcagcggcg tttgagcgat ttcagtcctt cgatgacggc agtcagttcc
      121 atgcggttgt tggtggtttg cgcttcgccg ccgaaaagtt ctttttcgtg gctaccgtag
      181 cgcattaaca cgccccagcc gcccgcgccg ggattgcctt tgcacgcgcc gtcggtgtaa
      241 aggtaaacgg tttggtccat tattgtgcct ttgtgtggtg tccagcgtga ttataagcgg
      301 tttgtgcgcc gtgccgtctg aaagattgcg gcggattaaa ataggcatat aatgtgcaac
      361 tttatttttg aatatgaagg agcggatagt gggtcaaagc ggcgagttgt tttgtttcgg
      421 acagatgccc gtatggaagg cggaaaacct gccggaaatt ttgttgtcgg gttattcgtc
      481 cgaaggaggg gaatgggtct gcctgaatgt gttgcagggc gatgtcgaag tccgcacgca
      541 ggacggggcg gcagaggttt ggtcggcgga aagcggcgat tgcgtgtttg cgccgcagca
      601 ggtgttttcg gtcaaaccga aaacggatga tgctgaaatc cgtttgtcga tgtattgcac
      661 ggcggcagac tattttcaca aaaaatacgg gatgagtgcc acgcattctg cggtcgtggc
      721 ggcacaggat accgtaccgg cgggcagggc gttggatatg ggctgcggac aggggcgcaa
      781 cgcgctgttc ctcggcttga aagggtttga cgtaaccgct gccgatcaca atccggccgc
      841 gttggcaaac gtggcggagc tggcagaggc ggagggtttg aacgtccgca cgctggaata
      901 tgatttgaac gccgccgcct tgcagggcga atttgattat attgtggcaa cagtggtgct
      961 gatgttcctg atgccgcagc gcgtgcctga cgtgattgcc gatatgcagg cgcatacggc
     1021 ggcgggagga tacaatttga tcgtatcggc aatggatacg gcggatttcc cctgtccgat
     1081 gccgttccct tttaaattta aagagggcga gctgaaggat tattatcggg attgggaact
     1141 ggtcgaatat aaagaagaat tgggttctat gcatgccaaa gacgaaaacg gcaatccgat
     1201 acggtttaaa tttgtaacga tgctggcgaa aaagcctgaa taatcctcaa aaatgccgtc
     1261 tgaacatttt gtttcagacg gcatttttac gtctagtccc gatacgccca atgattgacc
     1321 gggccgtgtc ccgcgccgat ttccaaaggg tttgagattg ccgccgtaat gtaggacttg
     1381 gcagtccgta cggctttgca aacgtccgag ccttttgcca actcggcggt gatgcaggcg
     1441 gaaaacgtgc agcccgtgcc gtgcgtgtgg gcggttggaa agcgcgggct gtccaattcc
     1501 agcgtttcat tttgcgtaaa cagccaatcc gtgcagcgtc cgcttgtgct gccgttcaaa
     1561 tgtccgcctt tgataacgac atttttgaca ccgtaatcaa gcaggatttt tgccgcacgt
     1621 tccgcatctt tacggttttc gatatgcacg ccggtcaggg cttccgcctc gggcaggttg
     1681 ggggtcaata catccgtatc gggaagcagc aggcgcgtca gtgccgcaac ggcggaatcc
     1741 tgcaacagcg gcgcaccgcc tttggcaatc atcacagggt cgagtacgcg cctgccaaag
     1801 ctgcagtgtt tcagcttgtc ggcaacgcat tcgatgattt ccgccgtgcc gagcataccg
     1861 attttgtagg cgcggatgtc gaagtcttcc ctgattgctt ggatttgtgc ggtgatggtt
     1921 tcggtcggga cgagatgaac cgccgacacg cccaaggtat tttgcgcggt aacggcggtg
     1981 atgacgcacg ttccgaacac gccgcgcatc tgaaatgttt tcaaatccgc ctgaatgccc
     2041 gcaccgccgc ccgaatccga accggcgatg gtaagcgttt gcacaaaaga gcttttcatt
     2101 tgttcctcct taatattgcg tctgataaaa agggctaatg cctgtaaaaa agcacggcga
     2161 tgccgtctga aaaccgcagc gcgggggttt gtggtgcatt atatgccgta ttgcaggaca
     2221 aggcacaggg cagggcggcg gggtgcatcg tagcaactgt atttttgccc gacggggtga
     2281 aaaatacagt tgctacaccc actgataatg aaaaaagaga gcaatgccgt ctgaaccgcg
     2341 tgttgcggcg agcataaacc ggcacgcttt attcgtcatt tccacaaaaa cagaaaccta
     2401 aaattccgtc attcctacga aagcgggaat ccagacctgt cggcacggaa acttatcgga
     2461 taaaacggtt tcttcaaccc tacgctctag attcccgctt ttgcgggaat gacgaagttt
     2521 cagacggcat caaatgcttt gtcaggcttc agacggcatc gtccaccctc tgccgtc
//
LOCUS       DAWHRS010000196         2555 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWHRS010000196 DAWHRS010000000
VERSION     DAWHRS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.196"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            544..2346
                     /locus_tag="WIB82_001990"
     CDS             544..2346
                     /locus_tag="WIB82_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ3843236.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANATDEEDDEELEPVVRTAPVLSFHSDKEGTGEKEEVGASSNLTVYFDKKGVLK
                     AGTITLKAGDNLKIKQNTNENTNENTNASSFTYSLKKDLTDLTSVGTEKLSFSANGNK
                     VNITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKK
                     RAASVKDVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGK
                     KTEVKIGAKTSVIKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKT
                     TTANGQTGQADKFETVTSGTNVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQ
                     LQNSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETININAGNNIEITRNGKNIDIATS
                     MTPQFSSVSLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQL
                     KGVAQNLNNRIDNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGEAGYAIG
                     YSSISDTGNWVIKGTASGNSRGHFGTSASVGYQW"
BASE COUNT          803 a          636 c          615 g          501 t
ORIGIN      
        1 acgcaacagg aatttatcgg aaaaaacaga aacccccacc gcgtcattcc cgcgcaggcg
       61 ggaatccaga aacacaacgc ggcaggactt tatcggaaaa aacagaaacc ccaccgccgt
      121 cattcccgca aaagcgggaa tccagacccg tcggcacgga aacttaccgg ataaaacagt
      181 ttccttagat tccacgtcct agattcccgc tttcgcggga atgacgagat tttagattat
      241 gggaatttat caggaatgat tgaatccata gaaaaaccac aggaatctat cagaaaaaac
      301 agaaacccca ccgccgtcat tcccgcaaaa gcgggaatcc agacccgtcg gcacggaaac
      361 ttaccgaata aaacagtttc cttagattcc acgtcccaga ttcccgcctt cgcgggaatg
      421 acgagatttt aagttggggg aatttatcag aaaaccccca accccaaaaa accgggcgga
      481 tgccgcacca tccgccccca aaccccgatt taaccattca aacaaaccaa aagaaaaaac
      541 aaaatgaaca aaatataccg catcatttgg aatagtgccc tcaatgcctg ggtcgtcgta
      601 tccgagctca cacgcaacca caccaaacgc gcctccgcaa ccgtggcgac cgccgtattg
      661 gcgacactgc tgtccgcaac ggttcaggcg aatgctaccg atgaagaaga cgatgaagag
      721 ttagaacccg tagtacgcac tgctcccgtg ttgagcttcc attccgataa agaaggcacg
      781 ggagaaaaag aagaagtagg agcaagttca aatttgacag tatatttcga caagaaagga
      841 gtactaaaag ccggaacaat caccctcaaa gcaggcgaca acctgaaaat caaacaaaac
      901 accaatgaaa acaccaatga aaacaccaat gccagtagct tcacctactc gctgaaaaaa
      961 gacctcacag atctgaccag tgttggaact gaaaaattat cgtttagcgc aaacggcaat
     1021 aaagtcaaca tcacaagcga caccaaaggc ttgaattttg cgaaagaaac ggctgggacg
     1081 aacggcgaca ccacggttca tctgaacggt attggttcga ctttgaccga tacgctgctg
     1141 aataccggag cgaccacaaa cgtaaccaac gacaacgtta ccgatgacga gaaaaaacgt
     1201 gcggcaagcg ttaaagacgt attaaacgca ggctggaaca ttaaaggcgt taaacccggt
     1261 acaacagctt ccgataacgt tgatttcgtc cgcacttacg acacagtcga gttcttgagc
     1321 gcagatacga aaacaacgac tgttaatgtg gaaagcaaag acaacggcaa gaaaaccgaa
     1381 gttaaaatcg gtgcgaagac ttctgttatt aaagaaaaag acggtaagtt ggttactggt
     1441 aaagacaaag gcgagaatgg ttcttctaca gacgaaggcg aaggcttagt gactgcaaaa
     1501 gaagtgattg atgcagtaaa caaggctggt tggagaatga aaacaacaac cgctaatggt
     1561 caaacaggtc aagctgacaa gtttgaaacc gttacatcag gcacaaatgt aacctttgct
     1621 agtggtaatg gtacaactgc gactgtaagt aaagatgatc aaggcaacat cactgttaag
     1681 tatgatgtaa atgtcggcga tgccctaaac gtcaatcagc tgcaaaacag cggttggaat
     1741 ttggattcca aagcggttgc aggttcttcg ggcaaagtca tcagcggcaa tgtttcgccg
     1801 agcaagggaa agatggatga aaccatcaac attaatgccg gcaacaacat tgagattacc
     1861 cgcaacggta aaaatatcga catcgccact tcgatgaccc cgcagttttc cagcgtttcg
     1921 ctcggtgcgg gggcggatgc gcccacttta agcgtggatg acgagggcgc gttgaatgtc
     1981 ggcagcaagg atgccaacaa acccgtccgc attaccaatg tcgccccggg cgttaaagag
     2041 ggggatgtta caaacgtcgc acaacttaaa ggtgtggcgc aaaacttgaa caaccgcatc
     2101 gacaatgtgg acggcaacgc gcgtgcgggc atcgcccaag cgattgcaac cgcaggtttg
     2161 gctcaggcgt atttgcccgg caagagtatg atggcgatcg gcggcggtac ttatcgcggc
     2221 gaagccggtt acgccatcgg ctactcgagc atttctgaca ctgggaattg ggttatcaag
     2281 ggcacggctt ccggcaattc gcgcggtcat ttcggtactt ccgcatctgt cggttatcag
     2341 tggtaagggc tttttcgcct gtctgctgtt ggggcaggcg gaaggtttga agggaagggt
     2401 ggcgatttgc cgcctgagac ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag
     2461 acatttaggg gattttccat gagcaccttc ttccaacaaa ccgcccaagc catgattgcc
     2521 aaacacatcg accgtttccc gctattgaag ttgga
//
LOCUS       DAWHRS010000197         2510 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWHRS010000197 DAWHRS010000000
VERSION     DAWHRS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.197"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..2030
                     /locus_tag="WIB82_001991"
     CDS             <1..2030
                     /locus_tag="WIB82_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3843237.1"
                     /translation="TYPDSSSSRIYLQNLKRKTDSNRPGRYSLADLSASDIQSKEPTF
                     TSRQTIIRLDGGVRHIQLDRNNEVTSFNGDNGTFGIVNEGSVVPESNEWKKVLLPWTV
                     RGFADDSKFKAFNKEKNNDNKPKYSQRYRIRENGNNGKRDLGDIVNSPIVAVGEYLAT
                     SANDGMVHIFKKGNGGDARDYSLKLSYIPGTMPRKDIQSQDSTLAKELRAFAEKGYVG
                     DRYGVDGGFVLRQVNNLNGQDRVFMFGAMGFGGRGAYALDLTKADGNDPTKASLFDVK
                     DNGNNGNNGNNRVELGYTVGTPQIGKTHNGKYAAFLASGYATKEITSGENKTALYVYD
                     LENNNGTPIAKIEVPNGKGGLSSPTLVDKDLDGTIDIAYAGDRGGKMYRFDLSSQSPD
                     QWTVRPIFEGTKPITSAPAISQLKDKRVVIFGTGSDLSEDDVDKMDEQYIYGIFDDDT
                     ETTGNVKVDLKGLGGGLLEQHLTQEDKTLFLTDYKRSDGSGNKGWVVKLKDGQRVTVK
                     PTVVLRTAFVTIHKYTGNDKCGAETAILGINTADGGKLTKKSARPIVPDANTKVAQYS
                     GDKKTSSGKSIPIGCMQKGNEIVCPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGS
                     GIDPAGKRSGKNNRCFSQKGVRTLLMNDLDSLDITGPTCGMKRISWREVFY"
BASE COUNT          711 a          614 c          654 g          531 t
ORIGIN      
        1 ccacctaccc cgacagttcg agcagccgca tctaccttca aaatctgaaa agaaaaaccg
       61 actccaacag acccggccgc tattccctcg cagacttgag tgcgtcggat attcaaagta
      121 aagagccgac tttcacaagt cggcaaacaa tcatccgctt ggatggtggc gtacggcata
      181 tccaactgga tagaaacaat gaggtcacta gttttaatgg cgacaacgga actttcggca
      241 ttgttaatga agggagcgtc gttcctgaaa gcaacgagtg gaaaaaagtg ctgctgcctt
      301 ggacggttcg gggttttgct gatgacagta aatttaaagc attcaacaaa gaaaaaaaca
      361 acgacaacaa gccaaaatac agccaaagat accgcatccg cgaaaacggc aacaacggca
      421 agcgcgattt gggcgacatc gtcaacagcc ccatcgtggc ggtcggcgag tatttggcta
      481 cttccgccaa cgacgggatg gtgcatatct tcaaaaaagg caacggcggg gacgcgcgcg
      541 actatagtct gaagctcagc tacatcccgg gcacgatgcc gcgcaaggat attcaaagtc
      601 aagactccac cctcgccaaa gagctgcgcg cctttgccga aaaaggctat gtgggcgacc
      661 gctacggcgt ggacggcggc tttgtcttgc gccaagttaa caacttaaat gggcaagacc
      721 gcgtgtttat gttcggcgcg atgggctttg gcggcagagg tgcgtatgcc ttggatttga
      781 ccaaagccga cggcaatgac ccgacaaaag cctctttgtt tgatgtaaaa gataacggca
      841 ataatggcaa taacggcaat aatcgcgtgg aattaggcta caccgtcggc acgccgcaaa
      901 tcggcaaaac ccacaacggc aaatacgccg ctttcctcgc ctccggttat gcgactaaag
      961 aaattaccag cggcgagaat aaaaccgcgc tgtatgtgta tgatttggaa aacaacaacg
     1021 gtacgccgat tgcaaaaatc gaagtaccca acggcaaggg cgggctttcg tcccccacgc
     1081 tggtggataa agatttggac ggcacaatcg atatcgccta tgccggcgat cgcggcggga
     1141 aaatgtaccg ctttgatttg agcagtcaaa gtcctgatca atggactgta cgccctattt
     1201 ttgaaggcac aaaaccgatt acttccgcgc ccgccatttc ccaactgaaa gacaaacgcg
     1261 tggtcatctt cggcacgggc agcgatttga gtgaggatga tgttgataaa atggacgaac
     1321 aatatattta cggtatcttc gacgacgata cggagacgac gggtaatgta aaggtagacc
     1381 ttaaaggttt gggaggcggg ctgctcgagc aacaccttac tcaggaagat aaaaccttat
     1441 tcctgaccga ttataagcga tccgacggct cgggcaataa gggctgggta gtgaaattga
     1501 aggacggaca gcgcgttacc gtcaaaccga ccgtggtatt gcgtaccgcc tttgtaacca
     1561 tccataaata tacgggtaat gacaaatgcg gcgcggaaac cgccattttg ggcatcaata
     1621 ccgccgacgg cggcaagctg accaagaaaa gcgcgcgccc gattgtgccg gacgccaata
     1681 cgaaggtcgc gcaatattcc ggcgataaga aaacttccag cggcaaatcc atccctatag
     1741 gttgtatgca aaaaggcaat gaaatcgtct gcccgaacgg atatgtttac gacaaaccgg
     1801 ttaatgtgcg ttatctggat gaaaagaaaa cagacggatt ttcaacaacg gcagacggcg
     1861 atgcgggcgg cagcggtata gaccccgccg gcaagcgttc cggcaaaaac aaccgctgct
     1921 tctcccaaaa aggggtgcgc accctgctga tgaacgattt ggacagcttg gacattaccg
     1981 gcccgacgtg cggtatgaaa cgaatcagct ggcgtgaagt cttctactga tttgcacgcg
     2041 aaaatgccgt ccgaaaggtt ttcggacggc attttttgcg tttttcggga ggggcgggtt
     2101 cgtaaaaggc gggctatagg gtaggcttca tctcgccaat ctcactgaat ccatcaattt
     2161 ccacaattca attaaatacc gtcaaaccga tgccgtcatt cccgtgcagg cgggaatcta
     2221 gaccttggga taacagcaat attcaaagtt tataaaagac ccgtcattcc cgctcaggcg
     2281 ggaatctaga ccttagaaca acagtaatat tcaaagatta tctgaaagtc cgggattttg
     2341 gattcccact ttcgtgggaa tgacggaatg caggtttccg tatggatgga ttcgtcattc
     2401 ccgcgcaggc gggaatctag accttagaac aacagtaata ttcaaagatt atctgaaagt
     2461 ccgggattct ggattcccac tttcgtggga atgacgtggt gcaggttcgt
//
LOCUS       DAWHRS010000198         2432 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWHRS010000198 DAWHRS010000000
VERSION     DAWHRS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.198"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            35..1390
                     /locus_tag="WIB82_001992"
     CDS             35..1390
                     /locus_tag="WIB82_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ3843238.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WIB82_001993"
     CDS             1394..2404
                     /locus_tag="WIB82_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ3843239.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     TYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          436 a          667 c          740 g          589 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcagatg agccgcatct tccagacgct
      181 cggtgccgat ccgcacagcc tcggctggtt ctttatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt tgggcggccg
      301 ccgtctgccg tatctgcttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcgaa tatggcgatg cagccgttta agatgatggt
      481 cggcgatatg gtcaacgagg agcagaaaag ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg ttcgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccgca gaccgtggtc gtggcgtttt atgtgggtgc
      661 ggcgttgctg gtgattacca gcgcgttcac gattttcaaa gtgaaggaat acgatccgga
      721 aacctacgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggat
      781 cgaactcttg aaaaccgcgc ctaaggcgtt ttggacggtt actttggtgc aattcttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccact gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttacctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgcat tggcccgagt tcggcaatat
     2341 ccgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg ac
//
LOCUS       DAWHRS010000199         2399 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWHRS010000199 DAWHRS010000000
VERSION     DAWHRS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.199"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            79..2292
                     /locus_tag="WIB82_001994"
     CDS             79..2292
                     /locus_tag="WIB82_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ3843240.1"
                     /translation="MTAISPIQDTQSATPQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QENYPADAATPYGEPLSDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          627 a          768 c          576 g          428 t
ORIGIN      
        1 attttagtaa cctatgttat tgcaaaggtc tcaagctgtt acaataaact cgtttttatc
       61 ggaacggaag accccatcat gaccgccatc agcccgattc aagacacgca aagcgcgacc
      121 ccgcaagaat tgcgcgaatg gttcgacagc tactgcgccg cgctgccgga caacgataaa
      181 aacctcatcg gtaccgcatg gtcgctggcg caggaaaact atcccgccga tgccgccacg
      241 ccgtatggcg agccgttgtc cgaccacttc ctcggtgcgg cgcaaatggt ccatgaactc
      301 gacctgctgc ccgatgccgt cgccgccacc ctgcttgccg acatcggacg ctacgtcccc
      361 gactggaacc tattggtttc cgaacgctgc aacagtaccg tcgccgagct ggtcaaaggt
      421 gtggacgaag tgcagaaact cacccacttc gcccgggtgg acagcctcgc cacgccagaa
      481 gaacgcgccc agcaggcaga aactatgcgg aaaatgctgc tggcgatggt taccgacatc
      541 cgcgtcgtgt taatcaaact ggcgatgcgt acgcgcaccc tgcaattttt aagcaacgcc
      601 cccgacagcc ccgaaaaacg cgccgtcgcc aaagaaaccc tcgacatctt cgccccgctc
      661 gccaaccgtt tgggcgtgtg gcagctcaaa tggcagctcg aagatttggg cttccgccat
      721 caagagcccg aaaaataccg cgaaattgcc ctgcttttgg acgaaaaacg taccgaacgc
      781 ctcgaataca tcgaaaactt ccttaatatc ctgcgtacgg aactcaaaaa atacaatatc
      841 cactttgaag tcgccggccg tccgaaacac atctactcca tttacaaaaa aatggtgaag
      901 aaaaaactca gcttcgacgg gttgttcgac atccgcgccg tgcggattct ggttgatacc
      961 gtccccgagt gttacaccac actgggcatt gtccacagcc tctggcagcc cattcccggc
     1021 gagttcgacg actacatcgc caacccgaaa ggcaacggct ataaaagttt gcacaccgtc
     1081 atcgtcggcc cggaagacaa aggcgtggaa gtgcaaatcc gcaccttcga tatgcaccaa
     1141 ttcaacgaat tcggtgtcgc cgcgcactgg cgttacaaag agggcggcaa aggcgattcc
     1201 gcctacgaac aaaaaatcgc ctggttacgc caacttttgg actggcgcga aaacatggcg
     1261 gaaagcggca aggaagacct cgccgccgcc ttcaaaaccg agcttttcaa cgacacgatt
     1321 tatgttttga ccccgcacgg caaagtcctc tccctgccca caggcgcgac ccccatcgac
     1381 ttcgcctacg ccctgcacag cagcatcggc gaccgttgcc gcggtgcgaa agtcgaaggg
     1441 cagattgtgc cgctgtccac cccgctcgaa aacggacagc gtgtcgaaat cattaccgcc
     1501 aaagaagggc atccttccgt caactggctt tacgaaggct gggtcaaatc caacaaggca
     1561 atcggcaaaa tccgcgccta catccgccag caaaacgccg acaccgtgcg cgaagaaggc
     1621 cgcgtccaac tcgacaaaca gcttgccaaa ctcacgccca aacccaacct gcaagagctt
     1681 gccgaaaatc tcggctacaa aaagccagaa gacctctaca ccgccgtcgg acaaggcgaa
     1741 atttccaacc gcgccatcca aaaagcctgc ggcacgctga acgaaccgcc gcccgtaccc
     1801 gtcagcgaaa ccaccatcgt caaacagtcc aaaatcaaaa aaggcggcaa aaacggcgtg
     1861 ctcatcgacg gcgaagacgg tctgatgacc acgcttgcca aatgctgcaa acccgcgccg
     1921 cccgacgaca ttgtcggctt cgttacccgc gatcgcggca tttcggtaca ccgcaaaacc
     1981 tgcccctctt tccgacacct cgccgaacac gcgcccgaaa aagtactgga cgcaagttgg
     2041 gcggcgttgc aggaaggaca agtgttcgcc gtcgatatcg aaatccgcgc ccaagaccgc
     2101 tccgggcttt tgcgcgacgt atccgacgcg ctcgcccgcc acaaactcaa cgttaccgcc
     2161 gtgcaaaccc agtcccgcga cttggaagcc agtatgcggt tcacgctcga agtcaaacag
     2221 gtcaacgacc tcccgcgcgt cctcgccagc ctcggcgacg tcaaaggcgt attgagcgtt
     2281 acccggcttt aaatacaaaa atgccgtctg aaagccgaat aacgcttcag acggcatttt
     2341 gattgccggg gtttgctatt ttttgttgca tagtcaatta aaaacaaaat agtacaata
//
LOCUS       DAWHRS010000200         2396 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWHRS010000200 DAWHRS010000000
VERSION     DAWHRS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.200"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..413)
                     /locus_tag="WIB82_001995"
     CDS             complement(<1..413)
                     /locus_tag="WIB82_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ3843241.1"
                     /translation="MTHISSPRNEFIRGIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAESLPILLIATVTFMINSRHILMGAALALHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
     gene            561..1013
                     /gene="dut"
                     /locus_tag="WIB82_001996"
     CDS             561..1013
                     /gene="dut"
                     /locus_tag="WIB82_001996"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ3843242.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1089..2276
                     /gene="dapC"
                     /locus_tag="WIB82_001997"
     CDS             1089..2276
                     /gene="dapC"
                     /locus_tag="WIB82_001997"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ3843243.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFEWVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
BASE COUNT          580 a          564 c          627 g          625 t
ORIGIN      
        1 ggcatattga atgcgggcaa accggctgct ttccgttttt ggatttcgga gaatgccatc
       61 gcccagcttt catcacacat aaaaaacagt gcgggcacgg cttttttcag cggtatttct
      121 ttcaggtgca gggcaagtgc cgcccccatc aggatatgcc gcgaattaat cataaaggtt
      181 acggtggcga taagcagtat cggcagagat tccgcccaca ggttgaccgt ggcaaactcg
      241 gagccgccgg cgaagttcat actggtcatc aacaacattt ccagccagct catgcctttt
      301 tgtccgccct gcataccgag tattaatgcc caaggcagaa gtccgataag tatgggcgaa
      361 ctttctttga tgccgcgtat aaattcgtta cggggcgagc ttatgtgtgt catattataa
      421 tattgatatg taagaaggtc gggcattata cacagacgga gtatttcaaa acaaaaccta
      481 tttgctgtgt ttcagtgtaa acacggcttg tgtataatcc accatcttta gacagaccga
      541 ccgtatgtgg gagcaaggca atgaatattg aagtagaaat gaaagtgttg gacgagagga
      601 tggcggattt tatccctacc tatgcgacag cgggttctgc cggtttggac ttgcgcgcct
      661 gtttggatga ggaagtcgtc ttgcagccgg gtgaaacgtt tcttgtaccg acaggtttgg
      721 caatttattt ggcgaatccc gcatatgccg ccgttttgct gccccgttcc ggattggggc
      781 ataaacacgg cattgttttg ggcaatttgg tcggcttgat tgactccgat tatcaagggg
      841 aattgaaggt ttcgttatgg aacagaagca gcgagccgtt tactgtcaaa ccgtttgagc
      901 gtatcgcgca gatggttatc gtgccaatcg tgcaggcgcg tttcaaacgt gtcgaggaat
      961 ttgtcggaag cagccggggt gagggcggct tcggcagtac gggtttgcat tgaatataaa
     1021 atgccgtctg aagggcacgt caggttcaga cggcatatct tccgatatac cgataaccgg
     1081 agaaaatcat gaatacctta ctcaaccaac tcaaacccta tccctttgcc cgactgcgtg
     1141 aagcgatgca gggcatttcc gcccccgaag gtctggaagc cgtcccactg catatcggcg
     1201 aaccgaaaca tccgacaccg aaagtcatta cggatgcgct gaccgcctca ttgcacgagt
     1261 tggaaaaata tccgctgacg gccggtttgc ctgaactgcg tcaggcgtgt gcgaactggt
     1321 taaaacgccg ttatgatggg ttgacgctga attccgacac cgaaattctg ccggttttgg
     1381 gaagtaggga ggcgttgttt tcttttgtcc aaaccgtgtt gaaccctgtt tcagacggcc
     1441 tcaaacccgt aatcgtcagc ccgaatccct tttatcagat ttatgaaggt gcgacacttt
     1501 tgggcggcgg tgaaatccat tttgccaatt gccccgcgcc gtctttcaac cccgattggc
     1561 gtagtatttc cgaagaggtt tggaaacgca ccaaactggt gttcgtctgc tcgcccaaca
     1621 accccagcgg cagcgtgctg gatttggacg gatggaaaga agtttttgat ttgcaggaca
     1681 aatatggttt cattattgcc tcggacgaat gctattccga aatctatttc gacggcaaca
     1741 agcctttggg ctgcctgcag gccgctgcac agttggggcg aagcaggcaa aaactgctta
     1801 tgttcaccag tttgtccaag cggtccaatg ttccaggcct gcgttccggt tttgtcgccg
     1861 gcgatgccga actgcttaaa aattttctgc tttacagaac ctatcacggc agcgcaatga
     1921 gcattcccgt acagcgtgcc agtattgccg cctggaatga cgaacagcac gttattgaca
     1981 accgccgcct gtatcaggaa aagtttgaat gggttattcc tatcctgcaa caggtatttg
     2041 acgtcaaatt accggatgcc tcgttttaca tctggttgaa agtccccgat ggcgacgatt
     2101 tggcatttgc acgcaattta tggcaaaaag ccgctatcca agtattaccc ggacgctttt
     2161 tggcacggga taccgaacag ggcaatcccg gggaaggtta tgtccgtatc gctttggttg
     2221 ccgatgtcgc aacttgtgtc aaagctgcgg aagacatagt ttcactgtat cggtaaggaa
     2281 tcggaaatgc cgtctgaact tttgttcaga cggcattttt caatattcta cggttgaatt
     2341 tgctgtaacg gtatttatag tggattaaca aaaatcagga caaggcgacg aagccg
//
LOCUS       DAWHRS010000201         2393 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWHRS010000201 DAWHRS010000000
VERSION     DAWHRS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.201"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(14..1216)
                     /gene="trpB"
                     /locus_tag="WIB82_001998"
     CDS             complement(14..1216)
                     /gene="trpB"
                     /locus_tag="WIB82_001998"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ3843244.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1299..1988)
                     /locus_tag="WIB82_001999"
     CDS             complement(1299..1988)
                     /locus_tag="WIB82_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ3843245.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            2185..>2393
                     /locus_tag="WIB82_002000"
     CDS             2185..>2393
                     /locus_tag="WIB82_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HEZ3843246.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRL"
BASE COUNT          533 a          688 c          625 g          547 t
ORIGIN      
        1 cagacggacg aggttacagt ttaatccctt tgagcttcgc gacggtattg atgtctttgt
       61 cgccacgacc tgagaggttg accaaaatca cttggtcttt acccattttc ggcgcatttt
      121 tcaccgccca agcaacggcg tggctggatt cgagcgcggg gatgatgccc tcgaagcggc
      181 agagcaagtc aaaggcttcg agtgcttcgt cgtctttggc aacagtgtat tcgacgcgct
      241 tgatgtcgtg cagatggctg tgttccgggc cgatgccggg gtaatccaag cctgcggaaa
      301 cagagtgcgt acccaaaacc tgaccgtttt cgtcctgcat cagatagctg cggaaaccgt
      361 gcaatacgcc aatcggtgcg cccgaagtaa tcggcgcggc gtgatcgggg gtgttcacgc
      421 ccaaaccgcc agcctccacg ccgacgaggc gcacgttttc ttcgccgata tacgggtgga
      481 acaaaccgat ggcgttcgat ccgccgccca cgcaggcaac ggcaacgtcg ggctgtctgc
      541 cgatggcttc ctgcatctgc gctttagctt cgttgccaat cacgcattgg aaatcgcgca
      601 ccatttcggg atacggcgcg gggccggcgg cggtaccgat gatgtagaac gtgtcgtcca
      661 cgcgggcgac ccattcgcgc atggcttcgt tcatcgcgtc tttcagcgtg cggctgccgc
      721 tttctacacc gaccacgttc gcacccaata atttcatacg gaacacgttg ggcatttggc
      781 gttggatgtc gtccgcgccc atatacacgt cgcaagtcat accgaagcgt gcggcaacgg
      841 tggcactcgc cacgccgtgc tgacccgcgc cggtttcggc gatgacgcgt tttttaccca
      901 tgcgttttgc caacagtgcc tgaccgatgg tgttgttgac tttgtgcgcg ccggtgtggt
      961 tcaagtcttc gcgcttcaac cagatttgcg cgccgcccag atgttcggac aaccgcgcgg
     1021 cgtggtaaac ggggctgggc ctgccgacat aatgtttcaa atcatggcgg aacgcttccc
     1081 aaaattcagg atcgtttttc gctgccttat aggcatccgc cagctcttgc aaggcgggaa
     1141 tcagggtttc ggagacataa agcccgccgt gttcgccgaa aaagcccttc tcgtcgggcg
     1201 cgtggtagtt cttcatcgga ttcttccttg tgcttcaaat tttcagacgg catttccgtt
     1261 tttgcaaaca tgccgtctga acgggaaatt gtcggcaatt ataactgttt ttcacacaag
     1321 cccgccattt caaaaaacgc ccgatacgcc gccgcttttt tcgccaaact caaaggatag
     1381 gcgcgcgaag tcgaaggcag gcgcgtcagc gtcaaatccc gtccggcaaa cggaaacggc
     1441 acggtttcgc ccgttttcgg cattttgata ccgccgccct gaatatcgag caggatttcc
     1501 gtcgccttgc cgcctgtcgt acaaatatgg cggcactcgg gtatttttgc caacaccgcc
     1561 gccaaatcga cggtttcaac tacctttaaa aacttgtcgg acgcattgcc gtgctgacgt
     1621 accgccttca aaacggtcgg acaggacgca atcccccgtt cgtgcaaaaa cgccttgatt
     1681 ttctcggcat caaacgcttt ttcagacaac ctttggaaat gcgccgcatc attaaaaaac
     1741 accagcccgt aaacgcgcca catatcgttt tggaaattcg gataatgaaa ctgcatcgcg
     1801 cgtttgtctt ccttgggcgg aaacgtcccc atcatcatga ccgcagcctt cggcggcaac
     1861 acggaatcga agggatgggt ttcgatttca agcagatttt cagacacgtt caatcctcca
     1921 acaggcaaaa cggcagacag ccggtttttg caccaaaccg ccaaacacgg caagccttca
     1981 aacagcatta tcacgccctg ccgttttcgg ttacaatgcc gacatcaaac ggatactgta
     2041 aacacattta tgtttcaaca cacaggacga cacataaagc accgccctat gtgccgtcct
     2101 gatttggaag gagttacgcc cctcccaaat agagtctgat tctaccgccc taaagggcgg
     2161 ggtttcaacc gaaaaggaaa cacgatgaaa gaacacaaag cccgcaagcg tttcgggcag
     2221 aattttttgc aggacacgcg gattatcagc gatattgtca acgccgtgcg cccgcaggcg
     2281 gatgatgtcg tgattgaaat cggtccgggt ttggcggcga ttaccgaacc tttggcgaaa
     2341 aagctgaacc gcctgcacgt tgtcgaaatc gaccgcgaca tcgtatgccg tct
//
LOCUS       DAWHRS010000202         2390 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWHRS010000202 DAWHRS010000000
VERSION     DAWHRS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.202"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(147..377)
                     /locus_tag="WIB82_002001"
     CDS             complement(147..377)
                     /locus_tag="WIB82_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843247.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(615..815)
                     /locus_tag="WIB82_002002"
     CDS             complement(615..815)
                     /locus_tag="WIB82_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843248.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            complement(819..1130)
                     /locus_tag="WIB82_002003"
     CDS             complement(819..1130)
                     /locus_tag="WIB82_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843249.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVMVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            complement(1142..2356)
                     /locus_tag="WIB82_002004"
     CDS             complement(1142..2356)
                     /locus_tag="WIB82_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ3843250.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKT
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLS
                     KVYHQNVDYDYF"
BASE COUNT          696 a          573 c          362 g          759 t
ORIGIN      
        1 accctttccg gcctgatgat ttttccgtcc tgggtaacag taggaggaag ggacgacaat
       61 aaatagtcgt ctgcctgcaa tcttgtatca aaacaattta tgccgacacg atagcccatt
      121 tatacgcccc tttttcttca ctctgtttat ttgacagatt tgatcatgct ccaagccatc
      181 ttgaagcctt ggattgccaa gatgaccgta atggccgcca tacccacggc ggaaaccatt
      241 gccacaaaac ccatgattac attcgccact tgcgtaccaa tcgcggatgc atcaaagcca
      301 tctgccataa caatagccgg tgtgaagata ccagctgcca acaatgcttt tacagcatat
      361 tttttaacga tgttcatcgt ttttttcctt tttttgatat ttaaaataag acgacttctt
      421 gacttgcttc atccggacga agtcttttcc gaatctcgtt tttagccgat aaaatagagg
      481 attgcgaaaa gaaaaagaaa catacagacc acccagccga taattagtgt tgcaaggttc
      541 attttcatga tatttttcct ttgttgcggg ctttgtgaaa ggttaacaga ccgcccgccg
      601 agcctgtttt tcttttattc cgattttacg aagaactgaa atatctggaa tcctccgccc
      661 atttcattta tgcctgaatt caacgcatct tcgtaacttt caaattgacc tgctgattta
      721 atattttgag taaaccccac atcaccgaac ggatcgggat aaataaagtc atgcgtttct
      781 aaatcttgaa ctatgaaacg ttcttcaaat ttcataaatc aacctttcgg cttttccgcc
      841 acctgaaaat caattaatga cggaaccatg cctttacctg tcgaagtcat ttcaaccatt
      901 accataactt cgcacggata tttgagattc tctaaccttg agaaattctt actatccccg
      961 aacttcattt gtgctgccgt aaatccaaca gcattacccg actgtgccgg taaaggtgtt
     1021 gcaaccaata cggaacaagt gtcaatgtta gagccatcaa tttcaccttt gaattttttg
     1081 gctcccaaaa aagttgcagg ataagttaca gtttgagttt gattaaacat tttgatttcc
     1141 tttaaaaata atcataatct acattttgat gataaacttt acttaaatat tcctgatatt
     1201 tcctttcttt ctcgacatca taatcaggac taaaaagcct tttttcccta tcgaatttgt
     1261 cagaattttt aaaagcaata acccccaatt catcaagttc aatttccaaa tcaatatccg
     1321 gctgttcatg aataaaaccg tgtttcaaac cgtcccttaa catttccaga gcatattttt
     1381 caggttctaa tcctttggga aatcccgaat ctgcctttaa agattccaca atttcgctat
     1441 tatcaaaacc catttcaatc atgaaattga ccagttttcc aaccgcgttt ttcgcgtaat
     1501 gcaatttatg ctcgaaagtt aaattaagcg ttttctttct ctgatcaaac ctttcgggaa
     1561 ccggcatatt tttaaatttt ctacaaattg gaaaagctcc acagaaatac gaaccctgat
     1621 ttattaaaat atccaagggt atttctatat ctccataatt aaactggatc tcgaacctta
     1681 cccatttgct ttctttatct ccaagctgcc tgcctttctc ataaacacga acaaaacgag
     1741 aatttttctt gcgacctaca taaaatgtct tgccgctccc gtcctcattc cgccaagccg
     1801 taccgatcgt ttcagatttc ggcctttgat tgctgttatc aaaaaaacca ttatcgtgat
     1861 ctaacaacgc ctgatccggc gtgtactctc catcaaaaaa atcaagtgct aggtcaattc
     1921 gcgttattct tgtccttatc gaatcatcga gaaactgctt tagcctcaac tcccaacccg
     1981 gacttgcaac gctgcaacca gtacctttca actcaactaa aacagtattg cgctgacctc
     2041 cgaaatgcac ctctccataa tcaacatcat ccgaacctaa cctatacatg gattcataga
     2101 atttgttgcc ccttgatttg catttgcgcg ttatgccaaa acctagaatt tcttccagct
     2161 ttctgcttaa tacatacatg tattcagcat cagaaaataa agggcaaccg gaaactttca
     2221 gtaaagtatc ttcatggaat gtgaatgaaa tccaatcaac aaaaaccccg tcttgcttac
     2281 ctcttcgctg cggaatttct aaaagcttcc cttttccgtc cgatataaag tgtgagaaat
     2341 attcaacttc actcattcgt tcactactct tggaattggt ttttaatttc
//
LOCUS       DAWHRS010000203         2375 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWHRS010000203 DAWHRS010000000
VERSION     DAWHRS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.203"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(120..1529)
                     /gene="dacB"
                     /locus_tag="WIB82_002005"
     CDS             complement(120..1529)
                     /gene="dacB"
                     /locus_tag="WIB82_002005"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ3843251.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTINDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLQITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMVVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1694..2260
                     /locus_tag="WIB82_002006"
     CDS             1694..2260
                     /locus_tag="WIB82_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3843252.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          552 a          574 c          674 g          575 t
ORIGIN      
        1 gacgttttaa caacagtgag ataaaagttc ttgttccatt ttaaaacaat tagtaaaatc
       61 aagtttgtcc tagttatcta ggacagcccc ttaaatttaa tccactatat tttcctgttt
      121 caggctcggc gttctttgca catcagtttc gcatccagcc agccatcgcc gccggagatg
      181 atgttgttgg caacgaagtt gtccaagtcg ggcagcaggg aaacggcgcg accgctgttg
      241 atgatgacga ccaccaccat cggtttgtcg cccagccagt agcctgccaa tgcgcggaca
      301 ttgttgagcg tgccggtttt caggcgcaga agcccaccgc tttgtttgaa gcggttgcgt
      361 aaagttccgt ctgtgccggc gatgggcagc gtgtcgatga aatcttgtgc aaacgggctg
      421 aaataagccg tttccaacat ttgcgccatc attctcgccg ttaccctttc ttttctagac
      481 agacccgaac cgttttccaa aaccaaatcc gcaacatcga tgcccgacac ggcaagttcg
      541 cgccggacgg cagacgctgc ctgttcggaa acggcgggca gtttgccgtc gccgccgagt
      601 ttgaggaaga cggaacgcgc aattagattg tccgaacgct tgttcatgtc cgtcaaaatt
      661 tccttcatcg gtttggcgtg tgcaacggca agcgtctgcg cgccttccgg cgtgtcggct
      721 atgccgatac cgtctgaaat ccgtccgccg ccgagcagcc agtggttggt aaaactttgc
      781 cggatcagtt cgtcaagcgc gaacatccgg acaccgacag gcttgcccaa acagctctcg
      841 ggaatattgc cgcgcaattt cagcgtattg tccgaaaaag atgcacgcat cagttttttg
      901 atcgaagggc aggcagcttg ggaggcggta atttgcaagt tgttttgggc gaaaatatgc
      961 ggcaaaggcg gatcggtgag gatgtcggta ctgtcggcgg cattgcgttc ggcgcgcacc
     1021 ataaccatac cggcagacag catagttgga ttggggggcg tcataaacgg cgaaccgctg
     1081 tcggcttcga aatcgtcggg gctgccgact tcgccccaca ggctgtggtc gagcatcagg
     1141 tgtcccgtga tattgcgtat gccttgttcg cgcaactggc gttggacggc aagcaggttt
     1201 tcctgattga aaacggggtc gccgctgccc gcccaataca ggtttccgtc aagcgtgccg
     1261 tcgtttatcg taccgttgct tttaaactcg gtcgcccagc ggtaattgct gccgaaggtt
     1321 ttgaaggcgg caaacgcggt aacgagtttc attgtggagg cggggttgac ggggacatcc
     1381 gagcggtggt caatgatgac ttttccgctg tcaagctctt ggacatatac ggcgatttcg
     1441 ttttgcggaa tgcgacccgt atcgagcgcg tgtgcggcga gggaggcgag aagcagcagc
     1501 agggaggccg ctgttttggg gaaattcata ggtaaatctg ttacataaaa aaagcaatta
     1561 taaggcaaag ccggataagt gggaacgctg tggcggcggg ccggcttgtt tgcaggggaa
     1621 atcacatata taataatcgc tatcattatg aaatgattga gacgcacaaa cttaatattc
     1681 aggaggaatg attatggcta agaaaatcag tattttggtg ggcagcctgc gccgtgcttc
     1741 ttttgcgcgc aaagtggcat tgaatgcggc ggagatgttc cccgaaggct ggcaggcgga
     1801 aatcgtcgaa atcggacatc tgccgcttta caatttcgat tatgacgacc ctgcggtgga
     1861 agatgtgccg ctgcccgaaa gctacacggt tttccgcgaa acgattaagg cttcggacgg
     1921 cattttgttc gttacgtccg aaaataaccg caccattccc gcctgtttga aaaatgcggt
     1981 ggacatcggc tcgaaaccga atgccgacgt ggcgtggaaa aacaaaccgg ccggcatcat
     2041 cagccattcc gtcggcaaga tgggcggtta cagttcgcaa aaaaacctgc gccttgcgct
     2101 gtcttatttc gatatgcccg taaccggaca gccggaagtg tttttgggca attcgccgac
     2161 actgtttgat gaaaacggca aattgatcga ctcggcaagg gattttgttc agtcatatat
     2221 taatcagttt gtcggtttga ttgaaagaaa tgccaaataa acaaacaagt cgaaaggccg
     2281 caaaaccggt tcgggttttg cggctttttt atatgggctg ggcagcgtca aggtttgccg
     2341 tttgccggat aatgcgcggc aaatgaccgg cgtgt
//
LOCUS       DAWHRS010000204         2352 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWHRS010000204 DAWHRS010000000
VERSION     DAWHRS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.204"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            125..2005
                     /locus_tag="WIB82_002007"
     CDS             125..2005
                     /locus_tag="WIB82_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ3843253.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPAGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          617 a          713 c          569 g          453 t
ORIGIN      
        1 tgttaatcca ctataacgca agcaccgcaa agacgcgcca accctctccc aacctttttt
       61 cagacggcat tttcggtaat ctgctaaaat cgcccgcttg agtttccaca gaaaaatccg
      121 aaaaatgaat attttttacg aagagtccgg ccaattcaaa atcgccgcca tcatccaaaa
      181 aaacgatgcc acctaccaag tcgataccca acacggcaag cgcaccaaag tgaaggcaaa
      241 caatgtcttt gccgagtttg acggcgatat ggcggcgttt ttggaaaacg cgcaggcaca
      301 ggcggcggac atcgacaccg atttattgtg ggaagtatgc ggcgaagagg aatttaccgc
      361 cgaagccatc gccgaagaat attacggcca tgcgccgacc aaaaccgagc tggcggcaac
      421 tttgattgcg ctttacgccg cgccgatgta tttctacaaa aaagccaaag gcgtgttcaa
      481 agccgcgccc gaagaaactt taaaacaagc acttgccgcc atcgaacgca aaaaacagca
      541 agacgcgcaa atcgacgctt gggcggaagc cttgaaacgc ggcgagatgc cgtctgaaat
      601 cgcggcggat ttgaaaacca tcctgcacgc gcccgacaag cagtcgctga cctacaaagc
      661 ctttaccaaa gccgccgacg cgctgaaaac ctctgcctac gaattggcga aaaaaacggg
      721 cggcattacg tccattcccc aatacctgca agacgggttt gaaatcaaat acttccccaa
      781 aggaacaggc tttcccgacc ttccccttcc cgaaatgccc gacctgccca aagccgatgt
      841 taccgccttt tccattgacg acgaatcgac caccgaagtg gacgacgctt taagcctgac
      901 cgacttggac aacggcacga agcgcgtcgg catccacatc gccgcgccgt cgcttgccat
      961 taaacaaggc gacaaaatgg aaaaaaacat catggaacgc ttgagcacgg tttatttccc
     1021 cggcggcaaa atcacgatgc tgcccgaaaa ctggattgcc gcgttcagcc ttgatgcagg
     1081 cgcacaccgc cctgccgtca gcatttattt cgatgtggac ggcgagttca acgtcggcgc
     1141 gccgacctgc aaaatcgaag cggtcaacat ctccgaaaac ctgcgtatcc aagccatcga
     1201 accgcatttc aacgccgaag cgggcttgga cgaagcgggc gaaatgatgt tcgcccacca
     1261 tcaagacctg atttggttct atcaattcgc caccgccctg caaaaagcgc gcggcaaata
     1321 cgaacccgac cgcgcgccgc aatacgatta cagcatcgaa ttggatgagg aaggcaaggt
     1381 atccgtcgtc cgccgcgaac gcggttcgcc catcgatacg ctggtcagcg agatgatgat
     1441 tcttgccaac agcacttggg cgcaaatgct ccatgacaac gacctgcccg gccttttccg
     1501 cgtccaaccg gcaggcaaag tgcgcatgag cactaaatcc gagccgcaca tcggcatggg
     1561 cgtgcagcat tacggctggt tcacttcgcc gctgcgccgc gccgccgact acatcaacca
     1621 aaagcagctg atcagcctga tagacgacac tgccgagccg ctgtatcaaa acagcgatgc
     1681 cgagcttttc gccgcgctgc gcgactttga tgccgcctat accgcctacg ccgacttcca
     1741 acgccaaatg gaagcctact ggagcctggt ttacctgcaa cagcaaggca tcagcgagct
     1801 gacggcgacg attctgaaag aagacctcgt ccgcatcgaa ggcctgccgc tggtcacgcg
     1861 cgcgaccggt attccgtttg acgcactgcc caaatcgcag gcattgttca aaatcaccga
     1921 attagatgcc gagaagcagt ttgtctcgct caactacatc aaggcagtcg cacccgcggg
     1981 tacaacggca ggcaatgccg tctgaagccc gatacggcaa actccggcaa aaacagaaac
     2041 ctgaatctca tcattcccac agtcgggaat ccggtttttt gggtttccgt tgtttttccg
     2101 tttcaatgaa cttccaagcc gccgttcccg ataaataccc gcaatctaaa atcccgtcat
     2161 tcccacgcag atgggaatct aggtctgtcc gcacgggaac ttatgcgccg tcattcccgc
     2221 gaaagcggaa atccagaccg ttcggcttca gttttttggt ttcgggcaac ttctgaatcg
     2281 tcattcccgc gcaggtggaa atataggtct gtccgcacgg gaacttatac gccgtcattc
     2341 ccgcgaaagc gg
//
LOCUS       DAWHRS010000205         2350 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWHRS010000205 DAWHRS010000000
VERSION     DAWHRS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.205"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..967)
                     /locus_tag="WIB82_002008"
     CDS             complement(<1..967)
                     /locus_tag="WIB82_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ3843254.1"
                     /translation="MAENGKYLDWAREVLHAEAEGLREIAAELDKNFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQCRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
     gene            1064..2119
                     /gene="tal"
                     /locus_tag="WIB82_002009"
     CDS             1064..2119
                     /gene="tal"
                     /locus_tag="WIB82_002009"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ3843255.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEIAALKQQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETKGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLA"
BASE COUNT          552 a          715 c          615 g          468 t
ORIGIN      
        1 tccgcgccgc caaaaggtcg tgcatattca gcgcgccaat cagcacgccg tctgcatcgg
       61 taaccagaag cccgttcaca tggtttgcct gcatgacttt cagggcttcg gtggcgagac
      121 gttcggcgga gatggtttta ggatgcgtat gcatgacttc gtctatcgaa agaccggtaa
      181 aattgtcgca ttcttgaaac aggcggcgca aatcgccgtc ggtgaatacg cctttcagac
      241 ggcattgccc gtccgttacc gccaacatgc ccagcccttt ttcactcatg ctgacgatgg
      301 cttctttcaa gggcgtgccg agtcggacgg caggcaggcc gccgcctttg tgcataatgt
      361 cggcaacgcg caaaagtagg cgtttgccga ggctgccggc aggatggctc aaggcgaaat
      421 cgtcgggcgt gaacgcgcgt gcgcgcagca ggacgaccgc caacgcatcg cccaaagcca
      481 tgacggcggt ggtgctggtg gtcggggcaa gccccagcgg gcaggcttct ttggaaaccg
      541 acgccgtgat gtggatgtcg gcatggcgcg ccatggttga atcggggcgg gcggtgatgc
      601 agacaagcgt gatgtctttg cgtttgagtg cggggatgat ggcggcgatt tcgtcgcttt
      661 cgccggaatt ggaaatcgcg acgaccacgt cgttgtccac aatcataccc aaatcgccgt
      721 gtgccgcttc cgcagggtgg acgaaaaacg caggcgtgcc ggtcgaggcc atagttgccg
      781 ccattttgcg cccgatatgt cccgacttgc ccatgcccgt gataacgacc ctgcccttgc
      841 agtgcaacaa cgcgtctgcc gcaaggacga agtttttgtc caattccgct gcaatttcgc
      901 gcaagccttc cgcttcggcg tgcaacactt cgcgtgccca gtcgagatat tttccgtttt
      961 ctgccattgc catccgcctt tgctgtaata aaactaccca aaatttccga atcggggtag
     1021 cataagccct caaaccccgc ctatccatcc aaaggaatca gagatgacca ttttatcgga
     1081 cgttaaagca ttaggacaac aaatctggct ggacaacctt tcccgctcgc tagtgcaaag
     1141 cggcgaattg gcgcaaatgt tgaaacaggg cgtgtgcggc gtaacttcca atcccgccat
     1201 cttccaaaaa gccttcgccg gcgacgcgct ttacgccgat gaaatcgccg ccctcaagca
     1261 gcaaaacctc agccccaaac aacgctacga aacaatggcg gttgccgatg tgcgtgccgc
     1321 ctgcgacgtt tgccttgccg aacacgaatc caccggcggc aaaaccggct tcgtcagcct
     1381 cgaagtttcg cccgaattgt ccaaagacgc gcaaggcacg gttgaagaag cgcgccgcct
     1441 ctacgccgcc atcgggtgca aaaacgcgat gattaaagtg cctgccaccg acgcaggcat
     1501 cgatgcgctc gaaaccctcg tttcagacgg catcagcgtg aacctgaccc tgctgttctc
     1561 acgcgcccaa accctcaaag cctacgccgc ctacgcgcgc ggcatcgcca aacgcttagc
     1621 agccggacaa agcgttgccc atatccaagt tgtcgccagc ttcttcatct cccgtgtgga
     1681 cggcgcgctg gacacaacac tgcccgacca cctcaaaggc aaaatcgcca tcgcccttgc
     1741 caaagccgcc taccaagact gggcgcaata cttcggcagc cccgaatttg ccgcgctgga
     1801 aaccaaaggc gcaaaccgcg tgcagctttt atgggcatct accggcgtga aaaacccggc
     1861 ctatcccgac acgctctacg ttgacagcct gatcggcgcg cacaccgtca acaccgttcc
     1921 cgatgccacg ctcaaagcct ttatcgacca cggcacagcc aaagccacgc tgaccgaagg
     1981 cgtagaagaa gcacaagcgc agctcgccga aaccgccgcg ctcggcatcg atgtcgaaac
     2041 cttggcaacg cgtttgcagg aagacggttt gaaacagttt gaagaagcgt ttgaaaaact
     2101 gctcgcgcct ttggcttagg cattttcccg atgccgtctg aaaggctttc agacggcatc
     2161 gcccgtttga ccggacacag tgcggcatat caaggattca ccctaaaaat attaatgggc
     2221 aagaagttaa tgcacgacat tacccactaa cacaaaagga aatagaatca tggcaaaagc
     2281 aatcgaaatc atttcaccca atgaaattta ttcagatctg atttttaagg atccggtacc
     2341 tccccatact
//
LOCUS       DAWHRS010000206         2262 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWHRS010000206 DAWHRS010000000
VERSION     DAWHRS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.206"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            413..>2262
                     /locus_tag="WIB82_002010"
     CDS             413..>2262
                     /locus_tag="WIB82_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein frpA"
                     /protein_id="HEZ3843256.1"
                     /translation="MADGYWALGLGIEAERIHNAQAVNNPNGSEWDNRKQLISALDKG
                     FDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIGGWGNGIINDLYKSVAKREWTGI
                     FEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKETKKRNGKYHVYDPLALDLDGDGI
                     ETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLA
                     DGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVWQDLNQDGISQTNELRTLEELGI
                     QSLDLAYKDVNKNLGNGNTLAQQGSYTKTNGTTAKMGDLLLAADNLHSRFKDKVELTA
                     EQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETD
                     SNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKAAIDAARDRIAV
                     LDAYTGQDSNTLYYMSEEDALNIVKVTNDTYDHLAKNIYQNLLFQTRLQPYLNQISFK
                     MENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEA
                     KKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYGNDG"
BASE COUNT          700 a          454 c          528 g          580 t
ORIGIN      
        1 gaatgagggt gaagttgttt taacaccaga acaaatccaa accttgcgtg gttatgcttt
       61 ccgtggcgat acctatggcg gttggcgtta tttggctaat ttgggtgacc gttatgcgga
      121 tgatgctgct gcaattgtcg gtaaggatgc aaacttaaat ggtttgaatt tatggatgaa
      181 aaaaggtgtg gaaaacctat gggatgatac ggtcggtaaa aagacccgtt tagagaaatt
      241 tgatcgggtt gcattgcaac atttcagcca atatgtagat ctaattaatg aaaataatgg
      301 tagattacct aacactagtg aaattgagag aagttactat aaagccgtta ccgaaaatgg
      361 tgtttcttct agtgcagcta ttgatttagt tattaatcgc tcacttccgg atatggcaga
      421 tggttattgg gcattaggtt tggggataga agccgaacgt atccacaatg cgcaagcagt
      481 aaataatccg aacggtagcg aatgggataa tagaaagcag ttaatatctg ctttagataa
      541 aggatttgat ggatctttta aagagaagca ttttactttt ttacaatctg tgataatgga
      601 tgtaacaaag ttaggtgttg aatatacaat agatggttgg caaaaaattg gaggttgggg
      661 taatgggata atcaatgatt tatataaaag tgttgcaaaa agagagtgga ctggaatatt
      721 tgagatcgtt aataataaca tcaagcaatt tagagatctg ttcccaaatc cggaaggctg
      781 gatcgatgat ggtcaccaat gtttcgctcc ttgggttaaa gaaactaaaa aacgcaatgg
      841 caaatatcat gtctacgacc ccctcgccct agatttggac ggagacggta tagaaaccgt
      901 tgccaccaaa ggctttgcag gcagcttatt tgatcacacc aacaacggta tccgcaccgc
      961 caccggttgg gtttctgccg atgacggttt actcgtccgc gatttgaacg gcaacggcat
     1021 catcgacaac ggtgcggaac tcttcggcga caacaccaaa ctggcagacg gttcttttgc
     1081 caaacacggc tacgcggctt tggccgaatt ggattcaaac ggcgacaaca tcatcaacgc
     1141 ggcagacgcc gcattccaaa ccctgcgtgt atggcaggat ctcaaccagg acggcatttc
     1201 ccaaaccaac gaactccgca cccttgaaga attgggtatt caatctttgg atctcgccta
     1261 taaagatgta aataaaaatc tcggtaacgg taacactttg gctcagcaag gtagctacac
     1321 caaaacaaac ggtacaaccg caaaaatggg ggatttactt ttagcagccg acaatctgca
     1381 cagccgcttc aaagacaaag tggaactcac tgccgaacag gcaaaagccg ccaatcttgc
     1441 gggcattggt cgtctgcgcg atttgcgcga agctgccgca ttgtccggtg atttggccaa
     1501 tatgctgaaa gcttattctg ccgccgaaac taaagaagca cagttggcat tgttagataa
     1561 tttgattcac aaatgggcgg aaaccgattc gaactggggc aaaaaatcgc caatgcgact
     1621 ttcaaccgat tggacgcaaa cggctaatga aggtattgca ctgacaccat cccaagtagc
     1681 acaactaaaa aagaacgctt tagtttccct ttctgataaa gctaaagcag ctattgacgc
     1741 cgcccgcgac cgcattgccg tgcttgatgc ctacacgggg caggattcca acacactcta
     1801 ttacatgagc gaggaagatg cgcttaatat cgtcaaagta accaacgata catacgacca
     1861 tctcgccaaa aacatctacc aaaacctgtt attccaaacc cgtttgcagc catatttgaa
     1921 tcaaatcagt ttcaaaatgg aaaatgatac gttcactttg gattttagtg gtcttgttca
     1981 agcatttaac catgtcaaag aaactaatcc gcaaaaagct tttgtggatt tggccgagat
     2041 gcttgcatat ggcgaacttc gttcttggta tgaaggccga agactaatgg ccgattatgt
     2101 ggaggaggca aaaaaagcag gtaaatttga agattaccag aaagtgttgg gtcaggagac
     2161 cgttgcatta ttagctaaaa catcgggtac gcaagcagat gatatcctgc aaaatgtagg
     2221 ctttggtcat aataaaaatg tttctttata tggtaatgac gg
//
LOCUS       DAWHRS010000207         2256 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWHRS010000207 DAWHRS010000000
VERSION     DAWHRS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.207"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..569)
                     /locus_tag="WIB82_002011"
     CDS             complement(<1..569)
                     /locus_tag="WIB82_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ3843257.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFSACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKR"
     gene            732..2138
                     /gene="dnaB"
                     /locus_tag="WIB82_002012"
     CDS             732..2138
                     /gene="dnaB"
                     /locus_tag="WIB82_002012"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ3843258.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAFNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          545 a          586 c          605 g          520 t
ORIGIN      
        1 cgtttggcga cttcgtccca gttgacgatt tcccaaaaac ctttcaggta gttggggcgg
       61 ctgttgcggt agtcgatgta gtaggcgtgt tcccacacgt cgcaggtcag cagcggcgtg
      121 ttttcagtgg tcagcggcgt agcggcgttg gaagtggaaa ccaaatccaa tccgccggca
      181 ggggttttca ccagccacgc ccaaccggag ccgaaagtac cggcagcgca agcggagaat
      241 gcttcttgga atttctcgaa gctgccccat ttcgcgtcga tggcggcggc cagttcgccg
      301 gaaggtttgc cttgaccttt gggcgtgaaa cccagccagt agaaagtgtg gttccaagtt
      361 tgggctgcgt tgttgaacac gccgcccgaa gattttttca caatctcttc caaaggcagg
      421 ttttcaaatt cggtgccttt gatttgattg ttcaggttgg tgatgtaggt ttgatggtgt
      481 ttgccgtagt ggaattccaa agtttctttg ctcagatgcg gggacaatgc gtccagttca
      541 taaggcagtt gcggcagctt atgttccatt ttgtgctcct aatattgttt taaatgttgt
      601 attttggcag tgttgctgca aatagctcag cagcgcacat attctacctg ttttgaggta
      661 cggaaaccaa ttaaacctgc tttacgctat aatagaagat tgcaatttcg gcacgacaga
      721 taggatgtac catgaacgat tacacagcta tgccgtctga agaccgtgag gtcggcgcat
      781 tgtcgctgcc tccacattcg atggaggcgg aacaatccgt tttgggcggg ttgatgctgg
      841 aaaatccggc ctgggacagg attgccgatg tggtttcggg agaggatttc taccgccacg
      901 aacaccgcct gattttccga tccattgcca aattaatcaa tgagagccgt cccgccgatg
      961 tgattacggt tcaggaagat ttgcagcgga acgaagaatt ggaagcggca ggcggattcg
     1021 aatacctgat tacgctggcg caaaacaccc cgtctgccgc caacatccgc cgctatgccg
     1081 aaatcgtgcg cgagcgttcc attatgcgcc aactcgccga agtggggacg gaaatcgccc
     1141 gcagcgcatt caatccgcaa ggcagggacg cggggcagct tttggacgag gcggaaaaca
     1201 aagtattcca aatcgccgaa agcacggcca aatccaagca gggctttttg gagatgcccg
     1261 atttgctgaa agaagtcgta cagcgcatcg atatgctcta ctcgcgcgac aatcccgatg
     1321 aagttaccgg cgtgccgaca gggtttatcg acctcgacaa aaaaacctcg ggccttcagc
     1381 ccggcgacct gattattgtt gccggtcgtc cgtctatggg taagaccgcc ttttccatca
     1441 atatcgccga acacgttgcc gtagaaggca agctgccggt tgccgtcttc tcaatggaga
     1501 tgggcggggc gcaactggtc atgcgtatgc tcggctcggt cggacgcttg gatcaaagcg
     1561 ttttgaaaac cggcaggctc gaagacgaac actggggtcg cctgaacgaa gcagtcgtca
     1621 aactctccga cgcgcccgtg tacatcgacg agaccccggg tctgaccgcg ctcgaactgc
     1681 gcgcccgtgc ccgccgtctc gcccgtcaat ttaacaataa gctgggatta atcgtcatcg
     1741 actacctgca actgatggca ggatccggcc gttccgacaa ccgagcttca gagctgggcg
     1801 aaatttcacg ttcgctcaaa gcgttggcga aagaattgca agtccccatc atcgccctgt
     1861 cgcaattgag ccgtaccgtc gaatcgcgta ccgacaaacg cccgatgatg tccgacctcc
     1921 gcgaatccgg cgcaatcgag caagatgccg acctgattat gttcatgtac cgcgacgaat
     1981 actacaacca ggactcaccc atgaaaggcc ttgccgaatg tatcatcggc aaacaccgca
     2041 acggtcccgt cggtaaaatc ttcctcacat ggacgggaca attcaccaaa ttcgacaatg
     2101 ctgcctatat tcccgaggag gcaaagatag aagattaaat ggctatataa aaatttatta
     2161 ggcgaaatca ggcaaaatcg tttaaaatca tgctgagaga ttgccctaaa aaataaaacg
     2221 cggtcttgag gcatttttgc attcagcccg catata
//
LOCUS       DAWHRS010000208         2249 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWHRS010000208 DAWHRS010000000
VERSION     DAWHRS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.208"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            109..1689
                     /gene="purH"
                     /locus_tag="WIB82_002013"
     CDS             109..1689
                     /gene="purH"
                     /locus_tag="WIB82_002013"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ3843259.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDILSGTPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLAAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRITARKAQDANFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT          575 a          679 c          588 g          407 t
ORIGIN      
        1 gtcagcaata tctgcaaaaa cctactgcca aaaaactaga aatagcggat aataccgccc
       61 cgaaaaagtt cccctttccc catccaactg tcagaaaagg aagccccaat gtccgtcatc
      121 aaacgcgccc tgatcagcct gtccgacaag gcaggcgcgg tcgaatttgc ccaaaccctg
      181 accaagctcg gtgtcgaaat cctctccacc ggcggcacgg cgaaactgct tgccgatgcg
      241 ggcgttcccg tgattgaagt cgccgactat accggcttcc ccgaaatgct cgacggccgc
      301 gtgaaaacgc tgcatccgaa aatccacggc ggtattttgg gtcggcgcga tttgggcgaa
      361 cacgtcgcca agatggaaga acacggcatc ggcaatatcg accttgtgtg cgtcaacctt
      421 tatcccttcg ccgccaccat cgccaaacca aactgcacac tggaagacgc gattgaaaac
      481 atcgacatcg gcggcccgac catggtgcgt tctgccgcaa aaaactggaa acacgtcgcc
      541 atcgttaccg acaccgccga cttccccgcc atcgccgccg aactcgaagc caacaacggc
      601 gcgttgagcg acaaaacccg cttcaacctg tcgcgcaaag cgttcagcca caccgcccaa
      661 tacgacggca tgatttccaa ctacctgacc tcgctttcag acgacatctt gagcggcacg
      721 cccgaaatcg gtgaattccc gagccagttc aaccaaagct ggattaaagt gcaagacatg
      781 cgctacggcg aaaacccgca tcagcgcgcc gcgttctacc gcgatattga ccccgccgca
      841 ggcagcctcg ctgcatacaa acaactgcaa ggcaaagaat tgtcttacaa caacatcgcc
      901 gatgccgatg ccgcatggga agccgtcaaa tcctttgacg cgccagcctg cgtgatcgtc
      961 aaacacgcca atccgtgcgg cgtcgccgtt gcagccgata cattgaccgc ctacaaactc
     1021 gcctacgtca ccgacaccac cagcgcgttc ggcggcatca tcgccttcaa ccgcgaagtg
     1081 gacggcgaaa ccgtcaaaca gattaccgac aaccagttta tggaagtctt gatggcgccg
     1141 aagtttaccg ccgaagccct cgaaatcgcc gccgccaaga aaaacgtgcg cgtattggaa
     1201 gtgccgcttg aggcaggcgc aaaccgcttc gaactcaaac gcgtcggcgg cggactgttg
     1261 gtacaaacgc ccgacatctg ccgcatcagc cgcgccgatt tgaaagtcgt ctccaaacgc
     1321 caaccgaccg agcaggaatg gaacgatttg ctgttcgtct ggaacgtcgc aaaatacgtc
     1381 aaatccaacg ccatcgtgtt cggcaaaggc ggacaaacct acggcatcgg tgcaggccaa
     1441 atgagccgcg tggacagcac ccgcatcacc gcccgcaaag cgcaggacgc caatttcgac
     1501 ctcaacggcg cgtgtgccgc ctccgatgcc ttcttcccct tccgcgacgg cgtggatgtg
     1561 attgccgaac agggcattaa agccatcatc catccggcag gttcgatgcg cgatcaagaa
     1621 gtcttcgacg cggcagacga acacggcatt gcgatggtgg taaccggcat ccgccacttc
     1681 cgccattaag gcagatgaac aaggcaatgc cgtctgaagg gctttcagac ggcattttgc
     1741 gctattttgt gaaggtaggg atgacggttc gggtattcct gacagggtgg attttcaagg
     1801 tgttgtatag ggtgtaggag gattcgtaaa aggtgggatg cagggtgtgc ttcagcccgc
     1861 tgcatcaaaa atttttggag aaccggcggg agtcggcggt tttggtttcg gcggggacgg
     1921 tggaaatggg taacattgac ggaatcgacg gaagcggtgg gctgaagccc acccttgtat
     1981 attggaatca ctgtatcata gcaacaaacc gcccggccgc cacccgcgcc cacccaaggc
     2041 acacaaccgt tgcgtagctc agggagcggc agggcaaccc atcgacacaa ccggacagtt
     2101 gccggacaac acaaccgaat gcaaggcagg ttgatgatga gtacccaata ccattacgca
     2161 ggtatagtga attaaatcta aaccagtaca gcgttggttc gccttagctc aaagagaacg
     2221 attctctaag gtgctgaagc accaagtga
//
LOCUS       DAWHRS010000209         2204 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWHRS010000209 DAWHRS010000000
VERSION     DAWHRS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.209"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1789)
                     /locus_tag="WIB82_002014"
     CDS             complement(<1..1789)
                     /locus_tag="WIB82_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ3843260.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQ"
     gene            complement(1802..>2204)
                     /locus_tag="WIB82_002015"
     CDS             complement(1802..>2204)
                     /locus_tag="WIB82_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ3843261.1"
                     /translation="TADNFRQHPDKDFSPKQPDLPLNDELSEEQLQTLLTTWTLYDGA
                     ALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSADLLAFIRKLDDDADFNPNRVIVFGS
                     NMASAMQHELDQAVRGYANKKEIELNVVIRV"
BASE COUNT          481 a          587 c          504 g          632 t
ORIGIN      
        1 tttgcaatat tttttgtttc agctcttcgg taaacttttt ggagatttct tcctgaaaag
       61 aattgtcgtt gatttcgccg acaagctgct tcacaaagtc tttttcgctg ctatcgacaa
      121 aataattcag tttgtacggt acatcgcgca cccgcgccat cagctcgttt accggcaggc
      181 gcaggccgcg tccgacttct tgcagcttgg aagtcgtgct gccgctggaa cgcagtttgc
      241 aaatctggaa aacgttggga ttgtcccagc cttcgcgcag cgtccatttg gaaaaaataa
      301 agcggcgcgg gttgtccaaa gacagcagtt tttccttatc gtgcaggatt tcattgattt
      361 cctgctcgat tctatcgtcg ctgtctgtat tgtctttgga aaaatagccg ccgtggcagg
      421 cggatacatc gtccaacgtc ttttgcaggt aatcgcggta aaacgggtcg ctttccgttt
      481 tcagacggcg tgccgcttcc gcgcgaatcc agctttcaaa tttatctttc aggctgcctg
      541 aaagctcgtt gccgctgcgg tagcccgcga tatcgtcaat aaaaaacagc gtcagcggct
      601 tgattttggg ctgtggcgcg cgttctgcca aaagcgcgcg ttccagcttg aaatgttcgg
      661 caaccgcccg ctgcatcatc gcatcctgca ccgtttgcga ataggaataa gggttgatga
      721 cggcacccgt tttcaactcc aagccgttgc ttaacaccac cacggtttta ttcattttgt
      781 cgattttcaa atccgaaata gccggatgga tttgcgccaa atcttcgcct tttgccagtt
      841 tgaacgtctg ctttttgtcc ttttcgttta attcaaattt cgcttctttg ccgtccgacg
      901 acaccagttt taccgccgca tccatgccgc cctgcatttc ttcctgaaac acgcgcacgc
      961 ctttgaccag cccgtcgtta aacgcgtcta ctgccgtcaa acggtaaagc aagttgtaat
     1021 attcatcgtt aaatgttgca ccgtagcgca aaatatattg cggttttaag cgtttgatat
     1081 tgccccacgt tttcgcgcta tctcgggtcg ggaatttatg cggttcgtcc acaatcataa
     1141 acgggcgcac ggcagccaat gcatcaacgg gattgtcaaa caaatccttc aatgccttgt
     1201 cgcccgtatc gttcatggac gacgaattaa ccatgcccgc gttaatcagc agcacatgaa
     1261 tttccttttt gttttccgct ttgacaaatt gctcaatcgt tatgggcgca ttggactttt
     1321 tgcccttatt ctttttcgcg ctttccacca cataggtttt caggcgtacg ccttcataat
     1381 cgccgccgaa atcctgttca aaatgctctg ccaaagcctt gctttgcaaa aactgctgtg
     1441 ttcccgcctt aatggacaaa gtcggcacga ccacgataaa tttgaacacg cccagccaac
     1501 ggtgcagctc gaacatggtt tgtgtgtagg tataggtttt gcccgtgccc gtttccatgg
     1561 aaatatcaag gatattttgg tcgtccgaac ggtcggggaa tcggccgtct ataccgtttt
     1621 ggctttgcac tttcaggata ttgtttgcgt attgttttgc agcaaacaaa agttcgggat
     1681 tttcgtctgc cgtccgatat ttgggcgttg ccccgtcaaa cacgcccaaa accgccgaaa
     1741 ccgcccgcat ttggtgcggc tggtttttct cgtaattaaa accgctcatg aattgcctcc
     1801 gtcaaaccct gataaccaca ttcaactcaa tctctttttt attggcataa ccgcgaaccg
     1861 cctggtcaag ttcgtgctgc atggcgcttg ccatattgct gccgaataca atcacgcggt
     1921 tgggattgaa atccgcatcg tcgtccagct tgcggataaa cgccaacaaa tcggcggaag
     1981 taaaaccggc attcatcaga tacagccgtt tttcgcacag atacgccgtg taagccccta
     2041 accgcacagg ctcaaccggc gtggtcagtg ccgccccgtc atacagcgtc caggtggtca
     2101 gaagcgtttg cagctgttct tcgcttaatt catcgttaag cggcaaatcc ggttgtttgg
     2161 gcgaaaaatc cttgtccgga tgctgcctga aattgtctgc cgtt
//
LOCUS       DAWHRS010000210         2186 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWHRS010000210 DAWHRS010000000
VERSION     DAWHRS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.210"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(30..836)
                     /locus_tag="WIB82_002016"
     CDS             complement(30..836)
                     /locus_tag="WIB82_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ3843262.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKRELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1056..>2186
                     /gene="ffh"
                     /locus_tag="WIB82_002017"
     CDS             1056..>2186
                     /gene="ffh"
                     /locus_tag="WIB82_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ3843263.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNNQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGIVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKV"
BASE COUNT          594 a          566 c          566 g          460 t
ORIGIN      
        1 gggactcaga cggcatctgt tggctcttct tatcttttca gaatgatttc caatacgaac
       61 ttgctgccca tataggcaat cataaggctg acaaatccga tgatggtcca cacggcggct
      121 tttttgccgc gccatgcggt catgctgtgc ttgagcagca gtccgccgta aatcagccat
      181 gacaatatgc cgaatacggt tttatgggta aaggtcatgg gtttgccgaa tacggcttcg
      241 gcaaaaaatg ttccgctgac gacggaatag gtcagcagga tgaaacctgc ccacatggcc
      301 tggaacatga gtttttccaa actgagcagc gacggcagga atcctgcgag cttggagaag
      361 ctcctgcggt gcagactccg attcagcagc agggtcaaaa cggacaataa tgttgcgatg
      421 ccgaacagcc cgtatgcgag cagcgaagtt ccgatatgca gcataaaggg aaagtcggta
      481 atttcatatc ccgagaattt tccaggaaaa accaaacctg acagcagcat cagtgcggcg
      541 caaggataca gcagcaactg cactccgcgc agcggataaa agaagctgcc ggcaaaataa
      601 ataaacagca tcatccaaac aatcaggctg ccggaatacc cgaagcccat aatgatgatt
      661 ttgtcttgaa tgaccggcat cagcagtgcc gcgccgtgga cggtcaatgc cgcgcccaaa
      721 accggcaatt ccctcttcca cgggtaatcc cgaccgcgcc cctgctgttg gcagtgccat
      781 gcaaatgcac ccaatcctgc gtaaaccgcc gtcaaaaaga tgaaaactgt cggcatggtg
      841 gactttctct atctatactg ttgcgccgtc tgcggccgct tatgaaatat tggaactttt
      901 aacgttggaa ttgtaaaatc cccgtttcgg gcaagccttg acggatttgc cgatatgctg
      961 tccggcacac aagccgcatc aaattatttt gattttattt taacaaagaa tgcccctgat
     1021 ggggcaagct attcttattc agaccaagga ccagtatgtt agacaattta accggccgct
     1081 tcagcaatgt cttcaaaaac atccgggggc aggccaaact gaccgaagac aatattaaag
     1141 aggccttgcg cgaagtccgc ctcgccctgc ttgaggcgga tgtcgccctg cctgtcgtca
     1201 aagagttcgt caacaacgtc aaagaaaagg ccctcggtca ggaagtggcg ggcagcctga
     1261 cgccggatca ggcatttatc ggcgtggtca acaaagccct gaccgaactg atgggcaggg
     1321 aaaacaaaac gctggatttg tcggttgcgc cgcccgccgt cgttttaatg gcaggtttgc
     1381 agggcgcggg caagacgacg accgtcggca aactcgcccg cctgctgaaa aacaatcaga
     1441 agaaaaaggt tttggtcgta tctgccgacg tttaccgccc tgccgcgatt gaacaactgc
     1501 gcctgttggc cgaacaggtc ggcgtggact ttttcccatc cgataccaac caaaaaccgg
     1561 ttgaaattgc aactgccgcc gtcgattacg ccaaaaaaca tttttacgat gttttgatgg
     1621 tcgataccgc cggtcgtctg gcaatcgatg aagagatgat gaacgaaatc aaagcccttc
     1681 acgcggcggt taacccggtg gaaactttgt tcgtcatcga tgcgatgctg ggtcaggatg
     1741 cggtgaacac tgctcaggca tttaatgaag ccctgccgct gaccggcatc gtgttgacca
     1801 agatggacgg cgattcgcgc ggcggtgcgg cattgtccgt acgccatgta accggcaaac
     1861 cgattaaatt tatcggcgtc ggcgaaaaaa tcaacggcct cgaacctttc caccccgacc
     1921 gtcttgccag ccgtatcctc ggcatgggcg acgtattgac cctgattgaa gacgtccaaa
     1981 aaggtattga tgaagaagcc gccgccaaaa tggcgaaaaa gctacaaaaa ggcaaaggct
     2041 tcgacctcaa cgactttaaa gaacaaatcc agcaaatgcg caatatgggc ggtttggaaa
     2101 acctgatgtc gaaaatgccg ggcgaactgg gtcaaatatc gaaacaaatc cccgaaggaa
     2161 cggctgaaaa agcgatgggc aaagtg
//
LOCUS       DAWHRS010000211         2185 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWHRS010000211 DAWHRS010000000
VERSION     DAWHRS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.211"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            476..1933
                     /locus_tag="WIB82_002018"
     CDS             476..1933
                     /locus_tag="WIB82_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3843264.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          514 a          635 c          506 g          530 t
ORIGIN      
        1 ctgcagatgc ccgacggtct ttatagcgga ttaacaaaaa tcaggacaag gcggcgaagc
       61 cgcagacagt acaaatagta cggaaccgat tcactcggtg cttgagcacc ttagagaatc
      121 gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actataatgc
      181 gcccttcggc gtggcggata tataaggaag tgattttcca tctaagtaaa aaccgcccta
      241 tcggataagc ccttaacaga aaaggcttta cccgcgccgt atcggaacac atcctctaaa
      301 atacaatccg ttgaattgaa aaaaatataa aaacatccgc ccgcgaaaaa cggcagcgcg
      361 tcgtttgaca aagaatgaaa atatcggtta aaaaccgatt ttcatacaaa aaacaccgcc
      421 gccgtccgca tccgtttcag acggtattga gagaaaatct tttaggagaa cctttatgtc
      481 ccggcatccc gcccccaccg gagaaaaaac attcttcggc caccccttcc agctttccac
      541 cctcttccat atcgaattgt gggaacgttt ttcattttac ggaatgcagg gcatcctgct
      601 gatttacctc tactacaccg ccgacaaagg cggcttgggc atagacaaaa ccctcgccgg
      661 cggcattgtc ggcgcataca gcggcagcgt gtacctgtcc accattttgg gggcgtggtt
      721 tgccgaccga gtatggggtg cggaaaaaac cctcttcctc tcgggcatcg tcgtgatgct
      781 cggacacatc gtccttgccg ccgccccggg cctgtacggc cttttaatcg ggctgatatt
      841 catcgcattg ggcagcggcg gcgtgaaatc tacggccagt tctatggtgg gcgcattata
      901 cgaacaggac gaaatgcgcc cgctgcgcga tgcgggattt tccattttct acatcgccat
      961 caacatcggc ggcttcctag gcccgctgct gaccggccta ctgcaggaaa acatcggttt
     1021 ccattatggt ttcggcgcgg cggcggtcgg tatggcattc ggcttgtggc gttattccct
     1081 gggacgtaaa aacctgcccc accccaccgt cccccatccg ctttcaaaag gacagggcaa
     1141 aactgcggcc gccgtcggca tcgccctcat cgccgcactt gcaaccgcca tcaaaaccgg
     1201 gcttgtcaac ctcgacaatt tctccggcat cctattatct accgtcatcc ttgccgtcat
     1261 cgcctatttc gcccgcctgc tgaccaaccc ccgcgtcagt tccgacaaca aacggcacat
     1321 catcgcctac atcccgcttt tcctgaccat ctgtatgttt tgggccgtct ggtttcagat
     1381 ttacaccgtg gcaaccgtct atttcgacga aaccgtcaac cgcaccatcg gttcgtttac
     1441 cgtgcccgtc gcttggaaag attctatgca aagcctgtgg gtcatcctgt tttccggact
     1501 gatggcggca atgtggacaa aaatggggcg caaacagccc aaaaccccgc tgaaattcgc
     1561 tatggcggta tttgttaccg gcgcgtcgtt tttgggattc gtccccttta tttcctccgg
     1621 tacgccgatg cctattgcgg ttttcgcact gatcgtcctc gccatcacga taggcgaact
     1681 gatgatttcc ccgattgcgc tgtccatctc caccaaaatc gcaccgcctt tattcaaaac
     1741 ccaaatggtc gcccttaatt tccttgcctt ttcattaggc ttcactttgg gcggcgtatt
     1801 gtttgaaaaa ggctatcagg cgggcgacga aatcggcttc tatcggctgc tgttctacat
     1861 cggcgcagcc acaggcttcc tgctgctcct gctcgtcccc aaattgaaca aaatgctcga
     1921 aggcacagac taagtcccgc cccgatgccg tctgaaccct tcagacggca tttttccgca
     1981 taatgaaacc aaaccgtttc cacccgacag gacaggctcc cgcccaaccg gaaggcagcc
     2041 tgccgattgt catttgaata acgcaaggga aagccgttga tttccgtttg tatggaaaca
     2101 gtttggtttc attggaaaaa ggcattttgt ccgactaaat tagtgctgca tcaacgaaat
     2161 atatagtgga ttaacaaaaa tcagg
//
LOCUS       DAWHRS010000212         2131 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWHRS010000212 DAWHRS010000000
VERSION     DAWHRS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.212"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..363)
                     /locus_tag="WIB82_002019"
     CDS             complement(<1..363)
                     /locus_tag="WIB82_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ3843265.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAA"
     gene            482..1303
                     /locus_tag="WIB82_002020"
     CDS             482..1303
                     /locus_tag="WIB82_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ3843266.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(1354..1800)
                     /gene="yciA"
                     /locus_tag="WIB82_002021"
     CDS             complement(1354..1800)
                     /gene="yciA"
                     /locus_tag="WIB82_002021"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ3843267.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
BASE COUNT          463 a          494 c          646 g          528 t
ORIGIN      
        1 tgcggcgttg agtttttcga cggcggcggc gaattttgtt ttttcggctt caaattttgc
       61 accatccgac caaacggcgg gcagggcgcg gccgttgcct tgcggatcgg actcaaaaag
      121 tgtgaacggt ttcttgctgc tttcggcaaa cgacgctgcc gcctgtttga atttttcgac
      181 atcgtaaggt tcttcgtctt tgaccatttt gcccatgcgt gtgaattcgg gcatcatgga
      241 tttgaacgcg gcggtgcggt tttcggaaat ttcgcctttg ggttgggaag gtattccgct
      301 gcctccgcag gcggaaagga gcagtgtgat ggcggcagca gcaaggctga tttgggtttt
      361 catattgaat gtgtcctgtc gtggtggtat ggttgttgtc attttcagtc ggcgcgatgc
      421 cgtctgaagg gtgttatgat acctgaaaca ggtttgggaa acgagaaaga aaggaaaaac
      481 aatggctgtt ttaattaccg gtgcttcggc aggattcggc gaagcgatgt gccgtgcgtt
      541 tgtcggggcg ggataccgcg ttatcggtgc ggcgcgccgt gcggacaggc ttcaggcctt
      601 ggcggatgaa ttgggtgctt tgttttaccc tttggaaatg gacgtgtcgc gacgcgagtc
      661 ggtggaaaac gccttaaacg gcatccccga tgaattttcc gacatcgact gcctcatcaa
      721 caatgccggg ctggctttgg gtctggacac ggcggacaag gcggattttg aagattggga
      781 aacgatgatt caaaccaatg ttttgggttt gacgttcctg acgcgcaaaa ttttgccgca
      841 aatggtggaa cgcggcggcg gttatgtgat gaatttgggt tcgattgcag gcaattatgc
      901 ttatgccggc agcaacgttt acggggcgac caaggcgttt gtgcgccagt tcagcctgaa
      961 tttgcgcgcg gagttggcgg ataagaacat ccgcgttacc aatatcgagc cgggtttgtg
     1021 cggcaatacg gagttttcca atgtgcgctt caaaggcgat gacgagaggg cggcgggcgt
     1081 gtatgagggt gtggaattta tccgccccga agatattgcg gaaaccgcat tgtggctgta
     1141 ccagcggccg gcgcatatga atgtgaacac gattgaaatt atgcccgtgg cgcagacttt
     1201 tgcaggaatg aaagtgataa aaaaagccgt gcccgaagtg cgggaagact ttgaaaaaca
     1261 gagtatgtcg ctgttttccc gcatcaggtc ctggttcaaa tgatgcaaaa tgccgtctga
     1321 agacagtttc agacggcatt tttacgggta tttttacgga gtaggcaata agcccgccaa
     1381 tttggggttg ccttctttcg gaatcgggcg cggattgcct tccgcatcga tggcaacata
     1441 agtgaacacg gcttcggtta cgaggtagcg gtcttcggta acgcaatcgt tcatcaaagt
     1501 tttcacccag acgtcgactt taagctggag ggaagtgttg cccacgcgga cgcaatgccc
     1561 gtagcagcag acgacgttgc cgaccttgac cgggcggatg aagttcattt cctgaacggc
     1621 gacggtaacg atgcgtcccc gcgcgatttc cgccgccaat atgccgccgc ccaaatccat
     1681 ctgcgacata atccagccgc cgaaaatgtc ttggttggga ttggtatcgc gcggcatagc
     1741 gacggtacgc aggagcagtt cgccttgagg gcgttggcgg ttgccttctt cgtgctgcat
     1801 aaagtttcct tgttttattg aaatataaat cgaacctgca cccctgcccg aaacgggaag
     1861 tcgggaagtg tgtagtttaa cccatttgag acatacaagg gcgggcgcgg aacagattcg
     1921 caaggcgtat tgtagggcgg ggctgtagag tgggcttcag tccgccaatc ccgccaaatc
     1981 ctaccctaag caactgaacc gtcattccca cgaaagtggg aatctagaat ctcgggggtt
     2041 cagtcatttc cgatagattc ccgccgcgtc agggggtctg gattcccgcc tgcgcgggaa
     2101 tgacgaatcc atccatacgg aaacctgcac c
//
LOCUS       DAWHRS010000213         2118 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWHRS010000213 DAWHRS010000000
VERSION     DAWHRS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.213"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(78..2093)
                     /locus_tag="WIB82_002022"
     CDS             complement(78..2093)
                     /locus_tag="WIB82_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ3843268.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEVNAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWNERQLTQSGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLRVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
BASE COUNT          442 a          638 c          582 g          456 t
ORIGIN      
        1 cattaaaaaa ggtcgtctga aaaagcagtt tcagacgacc ttttttcatt cgcggcggca
       61 gtgccgctcc cccgccctta gcccaaaaac tctttcaaaa acagcaagac gcaggcgagt
      121 tcgtcggcgg attcgcgttg ggtgccgttg ccggtatggc cgccgccgtc aggcgagtag
      181 agccaagatt gcgcggaggt ttcgcgcagt ttggcgtaga acttgagcgc gtgggcggga
      241 tggacgcgat cgtcggacag gctggtggta atgagcgcgg gcggataatc gatgccgtct
      301 gaaagattgt gatacggcga caattcgccc aaccggcgtt tgcagacttc gtatttttgc
      361 ggattgccgt attcgtctgt ccagcttgaa ccggcggaga gcagcggata acggatcatg
      421 tcggtcagcg gcacttcgca caccagcgcg ccgatgcttt gcggttcgcg cacgaaggcg
      481 gcggcagtaa tcagtccgcc gttgctgccg ccctgcaagc cgatgtgttc gggcgaactg
      541 ataccgcgtt cggacaaatc gcgcacgact gccaataaat catcaacgct tttatgtttg
      601 ctgattccct gcgccgcctg atgccagcgc gggccgaact cgccgccgcc gcggatgttc
      661 gccaatacaa aggcattgcc ctcttccagc caatatttgc caatgctgcc cagataatgc
      721 ggcaattcgg gaatgccgaa accgccgtag gcatagacca gcgtcggcat gtcgggcgcg
      781 gcgtttttgc cgacgtggaa ataaggaatg cgctcgccgt cagccgaagt cgtccaaaac
      841 tgctgcacgt taatgccgtc tgaatcaaac tgctgcggct ggcggcgcat gacggtcagt
      901 tccatcacgt tcaaatccag cgcaaacagc gtcagcggcg tggtgaaatc gctggcggca
      961 aggtaaacca cgtcgccgcc ccaaggttgg tcggtcattt ccaacgcgcc cgaaggcagg
     1021 cgcggcaatt cgacttcctg ccatttgccg tcggcaaacc gccatgcttt cagacggcct
     1081 tgtacgttct ccaacaggct cgccaccaca aaacgcttgg tcgtttccac gctttccaat
     1141 gcctgcgttt catcgggcgc aaacaaaagc tgcgccgccc cgagttcgcc ccgattcagc
     1201 ttcaccgcca ccagcgcgcc gctcggatag ctttggttcg cgcggttcca gtccttgcgc
     1261 agcgtcagca aaagatgccc cgccagatag ccgaccacgt cgcaatcgtt gggcaggttt
     1321 aacggtttcg cctcgccttc ggctgagacc cgcaaatagg ttttggtgta aaaaccgtcc
     1381 gacgcttcaa tcaaatcaat cggcgaaccc tgcggatcga gataacgcca cgcgttcacc
     1441 atcatgccgt cttcgccgat ttgatacaca ggcaggcttt cctcgaaact cttgccgcgt
     1501 tccaccagcc atacttcgcg cggatagccc gattgggtca actggcgttc gttccaagcc
     1561 ggacacaccc acacgctgtt ttcatcgcgc cacgacacat ggtttttgcc tgccggaaag
     1621 tgaaaaccgc cttctaccaa ttcccctgct tccaaatcca cttccagcgt gtacgccgta
     1681 tcaccgcccg acttgctcag tgttaacaac gcgcggttgg gcttttccac caagtgcgat
     1741 acgccgccca aatacacatc atcgccgagc aattcatcga aatccgccac tgaaaacagg
     1801 attttccact cgggatagcc ggaacggtaa gtcgccgccg tacacacgcg gtacacgccc
     1861 ttcggatatt ccgcgtcctg atggaaatgg tacatccgcg cgcggtgttc ctgacaaaac
     1921 ggaatctgcc gcgtgtcctg catttgattc aaaatgccgt ctgaaagcgc gcgcgccttg
     1981 tcgttgttta aaaaacgcgc gcgcgtttcg gcattcactt cagcagcgaa gttttgcgtt
     2041 tcggcggaat cgaggttttc aaaatggcgg taggggtcgg ggtaggattt catcggggtc
     2101 cttgagggtt gggcaggt
//
LOCUS       DAWHRS010000214         2089 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWHRS010000214 DAWHRS010000000
VERSION     DAWHRS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.214"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..193)
                     /locus_tag="WIB82_002023"
     CDS             complement(<1..193)
                     /locus_tag="WIB82_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3843269.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQN"
     gene            complement(212..856)
                     /locus_tag="WIB82_002024"
     CDS             complement(212..856)
                     /locus_tag="WIB82_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ3843270.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(1021..1839)
                     /gene="opc"
                     /locus_tag="WIB82_002025"
                     /gene_synonym="opcA"
     CDS             complement(1021..1839)
                     /gene="opc"
                     /locus_tag="WIB82_002025"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ3843271.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHISVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT          575 a          438 c          510 g          566 t
ORIGIN      
        1 tgttttgaac aataatctgc ccgtcgtcga gcttttgcaa accggcgagg atttttaaaa
       61 acgaagactt gcctgcgccg ttgcgtccga ttaagccgac tttttcgccg ctgtcgagtt
      121 gaaaagaagt tttgtcgagc aaggcaacgt ggccgatggc aaaagaagcg ttttctacag
      181 ataatatatt catgatacaa attctcaaca gttaccgttt ggattttacc gcaagtttgg
      241 cgcgggcaat ttcaaccgca cccggcagga cggaaacaat aatgatgccg cccatcacca
      301 agcccagatt gttttttacg acggggaagt tggcaaagaa atagcccgcg taagaaaaca
      361 ggataaccca caacaagcca ccgatgatgt tgtagcggat aaatttggca tagtgcattt
      421 tccccatacc ggcgacgaag ggggcgaagg tgcggacgat gggcataaaa cgggcaatga
      481 tgatggtttt gccgccgtgt ttttcgtaaa aacggtgggt tttatcgaga tattcacgtc
      541 ggaagatttt agaatcgggg ttggcgaaca gcctgccgcc gaaatatttg ccgacggtaa
      601 aattgagcgc gtcgccgagt atggcggcaa ggcttaataa tgcaaccatc aaatgaatat
      661 ccataccgcc cagcgcggca atcccgccgg cggcaaacag cagcgaatcg ccgggcagta
      721 agggcgtaac aatcaggccg gtttcgcaaa aaacaatcaa aaacagaatc gcataaatcc
      781 acacaccgta ttgcgccgac agcgcgagca ggtgttggtc gatatggagg atgaagtcga
      841 tggcggaggc aagcacggcg cgttcctaaa aaaaacaaac cgcgtatttt aaccgattgg
      901 aaaaatgccg tctgaaaagt ttcagacggc atcggctatt caaattcatt tcacgtaaaa
      961 accgcaaacc aaaatagttt gcggtttggc atttaaagtg acaatgatga tttcaaatca
     1021 tcagaatttt atgccgacgc gcaagccgta ttcacgaata ctggttttcg ggatggtgag
     1081 cgatacgtcg ccactcttgg ttgttacact aaactcgccg gattctttgt aagtgcgttg
     1141 tttgtagaac ggccccgcct cgatgctggc ggattcgccc agttttttac caatatttgc
     1201 acccaatcca aagccccagc cgttggtttt ttgattgatg tcttttttga gattggtaac
     1261 gccggtgttt aatttatagc gggaattgag gtcaaatttc acttcagacc aagggttgat
     1321 ataccagccg ttacccagtt gggaaagcaa atccgcgtga actttggcta accacgactg
     1381 acggctgctg tgaagcgtat gcttggtggt tttaatgctg tcttttgaag attcaaaacc
     1441 caagccggca cccacacgga aatttaaaga atcacttaac gtttgggtgt aggtgtagcc
     1501 tgtgtaaaga tcgatacggt tttcaggaac gccggtgggc agttttacat ttttagtctt
     1561 acccagcttg ttctcatctg tttccaaatt aataatattt tttttgctgc gcccgaaacc
     1621 ggcttccaag cggatgcctt ggttggcatc aaaaggaata tcagcacgca cgctgatgtg
     1681 tttggcagct ttgtgttttt ctttcaggaa agcacgagtt gaagaaatgg aagagaggtc
     1741 ggtgtggacg gtaaactcat tagcggtttg aagctcttgt gcagcggcgg cagtaccggt
     1801 cagggcaatc atggcacatg taaaaactgt ttttttcata gttaaaacct ctaaaatttg
     1861 gattgtagtc ggatatggta acataacgta aataatcgtt acgcttacaa ttatattctt
     1921 aagctttcgg gggggatttt acatatatta ataaaaatta acaaatagtt atttgtttac
     1981 aacgaattgt tattctcact tggttttctg ttttttatgg gaatgacgaa attttagttt
     2041 gtgtgtattt atcggaaaaa cagaaacccg ccgccgtcat tcccgcgca
//
LOCUS       DAWHRS010000215         2086 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWHRS010000215 DAWHRS010000000
VERSION     DAWHRS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.215"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(1..1761)
                     /locus_tag="WIB82_002026"
                     /pseudo
     CDS             complement(1..1761)
                     /locus_tag="WIB82_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          487 a          527 c          479 g          593 t
ORIGIN      
        1 ttgcaggtaa aaccgcacat tattgccttt tccgacaacg ccttccgcca caagcgcgtt
       61 gtagccgcct tcgacagaag ccgtccaacc tttggttttg tagcgttccg cacggttttc
      121 atcattgatg cgatgtttga aacgttggta ttgcaaccag ccgtccaaat acgcacccgt
      181 ttgtttatcg cgcaactgat gccacgcagc ataaacaccc ccgccataac catgcaaata
      241 actgcctgcc gcaccgcctt tgccgttgac tgatgcgtgc tggccggccc tgccgcccat
      301 cacgccgatt gccagtcggc tgccttcatt ttgccgtaca aacacctcgc cgccgatttg
      361 cacgcctttg cgccgcccgt ccgcagccgc gccgccccgt atattttgat gcgaccgacc
      421 gccgatgaag cgcagccaca atttttgacg ggacggatcg gcggcataca cgtcgtcgcc
      481 cctgtcggcg gcacgcagcc caaacaaagt atttgccgca taagcctgtt gggcatataa
      541 aaccgcttcc gcctcgggga tagccaaacg ctgcacgccc aagcaaaatc cgccttcgca
      601 ttgcttgagc aataattcgt acagtccctg ccggtaattt tgtttaaacg caaacgaatt
      661 agggtctgcc ttttccgccg caatcagttt gcgcccgtcg agcgttttca gattttccgg
      721 ccggcgttcc aaaaccacat tggattttcc gctgacgttt tgttcaaagg tcagggcggg
      781 ttcgtccgac tcccgcacgt cgtagccgaa caggaaatcc gcgccgccct gatacgcttt
      841 atggacggtc agtttttcat ctttcgtgtt caggcggatg cgggcattat cggaagtttc
      901 cagcgtatgg atgtcggaat ggcggcgcgg ttcccaaagg gaatctttca gcgtcatctt
      961 ggtcagttcc aacacctgtc cggcgacgtg ggatttgttt tcgattttga cgtgtaattt
     1021 tttattgaag atgttgttga gactagtctg tgatgtatta ctatgaggag tgagtacgga
     1081 atcttctacc aatttattaa ttaaatcggg catcgtctgt ttgagcgtac taaattttgt
     1141 tccaaactgt gctatatacg cctcctcagt ccgtttttta ttttctgccc aagcttccgg
     1201 tcttgttttg tataaatcct gtaattgact tttaaccgcc tgctttaaat cagccccata
     1261 cttctctgta atatattttt tgacaacggg catattatcg acggcgagga tgccgctatt
     1321 gtaattttta ccttggatat tgatttcgtc atacccatat tcggatgaat tttgatcggt
     1381 tttaccgctg gaaaaataat gggaagtgcc gggtttcggc ttgtctgaaa atgaaaaatc
     1441 ggcatcgtcg ccgattttgg tagaacgagt atttgaacca agctgagctc tgaacatacc
     1501 gaagcaatca aaagatatgc tgcacgggcc gcgccaaacg tgggaatttc tgtcttccgc
     1561 cgcttctaaa ataaatttgt ttttaatctc ggcttctttg acgggatcgt acgcatccga
     1621 acctcaaaca tatggcgtgc aaaacagcga taaaacagat aatgttaata gattaacccg
     1681 accgcttgct tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg
     1741 cttgcttgct ttcagtgtca tgagaaattt tcctttgtca agtgttaaaa ttataatgat
     1801 tatatactac attatactac atcgcaatga aaaatcagga aaagcaaaaa accctccgcc
     1861 gtcattcccg cgaaagcggg aatctagaat tttaatgccg caagaattta tcaaggatgg
     1921 tcggggttta aaggtctgga ttcccgcctg cgcgggaatg acggtttaga aatcacccga
     1981 aacaagaaaa agaaccgaaa ccgaacaagc cggattcccg cctgcgcggg aatgacggga
     2041 tttcaggtta ctgtttttga ttttctgttt tgtggaaata acggga
//
LOCUS       DAWHRS010000216         2056 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWHRS010000216 DAWHRS010000000
VERSION     DAWHRS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.216"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..968)
                     /locus_tag="WIB82_002027"
     CDS             complement(<1..968)
                     /locus_tag="WIB82_002027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ3843272.1"
                     /translation="MIGSGDTKQCKKFSADEFAKTIQSNFQSGIKYDGPTLATAGVSN
                     IIDEYLKDAYGGNGLKEAQRNLVAKEIADRIEAELHRQRANYQAQEAAKYSPAHGGVE
                     KSMYKHHADLQKTNTNIANRYSASANEYAAQAKSLGSLPKSVGYAALKNLGNALAALQ
                     MGEALYSNKANNITKTGSSILGGMLAVAAAGAIASLAGVTLPFWASIAVGASIGLGIS
                     AAWDAFGVGEKLGLDPNVPAFDPFFGWLYDKTGGWEGGIPDWAKEWLGLNRDGKYHQY
                     DPLTLDLDGDGIETVATKGFAGALFDHRNQGIRTATGWVAADDGLLV"
     gene            complement(1009..1443)
                     /locus_tag="WIB82_002028"
     CDS             complement(1009..1443)
                     /locus_tag="WIB82_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843273.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQPYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            1780..>2056
                     /locus_tag="WIB82_002029"
     CDS             1780..>2056
                     /locus_tag="WIB82_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3843274.1"
                     /translation="MKTTHCKLKKKVQKELLRFFVLEVTARSAADILGIQPDSAILFY
                     RTIRMVISHYPALAADEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVV"
BASE COUNT          629 a          445 c          336 g          646 t
ORIGIN      
        1 acaagcaaac catcatcggc agcaacccaa ccggtggcgg tgcggatgcc ttgattgcgg
       61 tggtcgaaca atgcacctgc aaagcctttg gtggcaacgg tttctatgcc gtctccgtct
      121 aagtctaggg ttagtgggtc gtattggtgg tatttgccat cgcggtttaa tcccaaccat
      181 tctttagccc aatcaggaat acctccttcc cagccgcctg ttttatcgta tagccaaccg
      241 aagaatggat caaaagctgg cacattaggg tctaatccta atttttcccc aacaccaaaa
      301 gcatcccatg ctgcactaat acctaaacca atagatgcac caactgcaat agaagcccaa
      361 aaaggcaatg taactcctgc taaactagct atagcacctg ctgctgcaac cgctaacata
      421 ccacctaaaa tagaagagcc tgttttggtt atattattag ctttatttga gtatagagct
      481 tctcccattt gcaacgccgc caaagcattt cccaaatttt ttagtgcagc atagccaact
      541 gattttggca aacttcctaa tgattttgct tgagccgcat attcattagc acttgcagaa
      601 taacgattag caatattagt atttgttttt tgaagatcag catgatgctt gtacatactt
      661 ttctccactc cgccatgcgc aggagaatat tttgcagctt cttgtgcttg ataattagca
      721 cgctgcctat gcaattctgc ctctattcta tccgcaatct ctttagctac aagattgcgt
      781 tgcgcttctt ttaacccatt tccgccataa gcatccttta agtattcatc tattatatta
      841 gatacgcctg ccgttgccaa agtcggccca tcatatttaa tacctgattg aaagttgctt
      901 tggattgttt ttgcaaactc atccgcagaa aattttttgc attgtttagt atcaccacta
      961 ccaatcattg ttattctcct tgagtttaag tcaaacaaaa aatgaaattc aaatactctc
     1021 tcttaactta ttgagatcaa tcatcaatat aaataaaaaa tataaactac cagaataacc
     1081 aaagccacga aaaatttttt ccaatatttc actatttaaa caaaaagcac cgcataccca
     1141 cgttgatgat tccgaaataa aacacatcca tatttccatt accgccaacc atgacactac
     1201 tatcctaatc aaaaatactt ttttatctgc ccaagcactt tttatattta acaatacaat
     1261 atgattttgt aatttaaaat ctaagctttg catttttcta accagtgcaa acaaacaacc
     1321 aagcgcaata aaaaaagatt ggtagggctg gtaaaaaggt agaaatagaa tatttaagac
     1381 caatggatag atccacatat acctatttgt taaattatta ataaattctt ttttgttatc
     1441 cataaaattt ccataaaata ttgcgatgat attgcactga gtttattagc aaacaacgta
     1501 aagaaaatat ttattatgat aagtgttttt taagtttcat tttcaaatga tggcgcgtaa
     1561 taaaaagttt cacaaatccg tttttcagac tatctaagta tctattttac aatctatttc
     1621 aataatcatt atgtcttaac aactcttctt ggcattatag atgctcactc atatcattta
     1681 atgatactct tgattaatct ttatgcgatt tagcgaaatt aaaatttctc ttttttgttt
     1741 gaggctgtac tagattagca gatatgttac cctcgaaata tgaagacaac acactgcaaa
     1801 ttaaagaaaa aagtacagaa agaactgctc cgcttttttg tacttgaagt taccgcccgt
     1861 tcagctgccg atattttggg catccagccc gactccgcta ttctgttcta ccgcacaatc
     1921 cgtatggtca tcagccatta tccggccttg gctgccgatg aggtttttga gggccctgtc
     1981 gagccggacg aaagcgattt cggcggacgg cgtaaaggta gacgtggtcg cggtgcagca
     2041 ggaaaagtgg ttgtct
//
LOCUS       DAWHRS010000217         1977 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWHRS010000217 DAWHRS010000000
VERSION     DAWHRS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.217"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(109..1419)
                     /locus_tag="WIB82_002030"
     CDS             complement(109..1419)
                     /locus_tag="WIB82_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ3843275.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYSA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1731..1868
                     /locus_tag="WIB82_002031"
                     /pseudo
     CDS             1731..1868
                     /locus_tag="WIB82_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          476 a          504 c          519 g          478 t
ORIGIN      
        1 ttgtctttat tggctttatc aataaacagg ataagacctg aaaaaaagcc gatacgcctt
       61 tttggtgtac cggctttgcc atactgttct gcttcagaca gcattgcttc attttgcctt
      121 taatacttct tcgtccagcg atttcaacca ttccagcttt tcgccgattt tgatttccaa
      181 cccgcgcggg acgggttggt agaagtccgg ttcgtccaag ccgtcgggca tatagctttc
      241 gccggcggag taggcgttcg gttcgtcgtg ggcgtagcgg tattcgcgtc cgtagcccaa
      301 ttccttcatc agcttggtcg gggcgttgcg caggtggacg ggcacttcgt cgctagcgtt
      361 ttctttgacg aagtggcgca tttggttgta tgccttgtag cccgcgttgg atttcgcggc
      421 ggcggcaaga tacaataccg cttgcgccaa agccagttcg ccttcgggcg agcctaagcg
      481 ttcgaaggtg gcggcggcat cgttggcgat ttggaaggcg cgcgggtcgg caagcccgat
      541 gtcttcccaa gcgatacgca cgatgcggcg ggcgaggtag cgcgggtcgg tgccgccgtc
      601 gagcatacgg cagaaccaat acagcgcggc gttcggatgc gaaccgcgca cggatttgtg
      661 cagggcggag atttggttgt agaaactctc gccgcctttg tcgaaacggc ggatttgcgc
      721 cccgagactg tcggcgagaa attcggcggt taagtttttc agacgacgtg tatcggcggc
      781 gcgtaaaagt tgttccaaca aattcaacaa tctgcgcgca tcaccgtcgg cggtattcac
      841 gagtaatttt tgcgcatccg tttcaatcgt aaactcttgg tattcaggca aagccaatac
      901 cttggcaatc agctttttca ggtcgtctga agacaagggt tgcaaaacat acacctgagc
      961 gcggctcaac agcgcgggat tgacttcaaa cgacggattt tccgtcgtcg caccaataaa
     1021 ggttagcaaa ccgctttcga catgcggcaa aaacgcgtcc tgctgcgcct tgttgaagcg
     1081 gtggacttca tcgacaaaca aaatcgtcgc gcgtccctgc tgcaaagcga tttcggcttt
     1141 atcgattgcc tcgcggatgt ccttcacgcc ggaaaatacg gcggaaacag gcaaaaactg
     1201 ggcgttgaaa ctctgcgcca aaatccgcgc caacgtcgtc ttgcccacgc ccggcggccc
     1261 ccacagcaac atagaatgcg gcttgccgcc ttctaccgcc acgcgcaaag gtttaccttc
     1321 gccgatgagg tgttcctgcc ccaccacgtc gtcaagcgta tgcggacgca atcgttcggc
     1381 aagcggcgcg tcgggttctc gggcaaacaa atcggtcata acggctccgt caacaggttt
     1441 tcaaacaata tgattatacg gcagggaacg gcggcgtgcc gcatacggat tccgcccctc
     1501 cgtttgcctt aagccgatat taggcgcata ctggaaaaga cgagagactt cacacaatat
     1561 atccggcacg gagaccgatt ccgcatcggc atgacaatac ccaaatcagc gtttcaatta
     1621 aacattaagg agactaaaat agaaaatttg ctttatctac cattgctttg ttgatttaat
     1681 cggcattatg ttttgaggcg gaagcccatg aatatactaa tattcaagag atggaatggg
     1741 tgtctttatt ttctgatccg caaagagacg atgatagtct tataaccctt aaagatgaaa
     1801 aaatcactgt aaaaaactat attgtgcctt ggtggaaaaa aggtgaaaac tttagaaaat
     1861 tagaacttgg cggattcgca tttgaagtgc aactttccct aacagaaaaa ggccagtatg
     1921 cggtagcata cggcctttcc tgcaagaaag attgccatga gctacacgca actgacc
//
LOCUS       DAWHRS010000218         1927 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWHRS010000218 DAWHRS010000000
VERSION     DAWHRS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.218"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..715
                     /locus_tag="WIB82_002032"
     CDS             <1..715
                     /locus_tag="WIB82_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ3843276.1"
                     /translation="QAMAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAV
                     FEDDVLLGEGAEKFLAEDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFP
                     LLESEHWGTAGYIISRKAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNP
                     ALCAQELHYAKFHDQNSALGSLIEHDRLLNRKQQRRDSPANTFKRRLIRALTKISRER
                     EKRRQRREQLIGKIIVPFQ"
     gene            727..822
                     /locus_tag="WIB82_002033"
     CDS             727..822
                     /locus_tag="WIB82_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ3843277.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            864..1706
                     /locus_tag="WIB82_002034"
     CDS             864..1706
                     /locus_tag="WIB82_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ3843278.1"
                     /translation="MQNHVISLASAAERRAHIAATFGRHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSSVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGEGAEKFLD
                     EDGWLKERFDPDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT          493 a          448 c          526 g          460 t
ORIGIN      
        1 acaggcaatg gcggaactcg tcccaggctt gtcggcgcac ccctatttga gcggagtgga
       61 aaaagcctgc tttatgagcc acgccgtatt gtggaaacag gcattggacg aaggtctgcc
      121 gtatatcgcc gtatttgagg acgacgtttt actcggcgaa ggcgcggaga aattccttgc
      181 cgaagacgct tggctgcaag aacgctttga ccccgattcc gcctttatcg tccgcttgga
      241 aacgatgttt atgcacgtcc tgacctcgcc ctccggcgtg gcggactact gcgggcgcgc
      301 ctttccgctg ttggaaagcg aacactgggg gacggcgggc tatatcattt cccgaaaagc
      361 gatgcggttt ttcctggaca ggtttgccgc cctgccgccc gaagggctgc accccgtcga
      421 tctgatgatg ttcagcgatt ttttcgacag ggaaggagtg ccggtttgcc agctcaatcc
      481 cgccttgtgc gcccaagagc tgcattatgc caagtttcac gaccaaaaca gcgcattggg
      541 cagcctgatc gaacacgacc gcctcctgaa ccgcaaacag caaaggcgcg attcccccgc
      601 caacacattc aaacgccgcc tgatccgcgc cttgaccaaa atcagcaggg aaagggaaaa
      661 acgtcggcaa aggcgcgaac agttaatcgg caagatcatt gtgcctttcc aataaaagga
      721 gaaaagatgg acatcgtatt tgcggcagac ggcaggatgc ggcggctgtt taccttgagg
      781 caatacttcg gcattttgca ccgattgctg aaaaaccgtt gaaaaacgcc gctttatcca
      841 acagacaaaa aacaggataa attatgcaaa accacgttat cagcttagct tccgccgcag
      901 aacgcagggc gcacattgcc gcaaccttcg gcaggcacgg catcccgttt cagtttttcg
      961 acgcactgat gccgtctgaa aggctggagc aggcaatggc ggaactcgta cccggcttgt
     1021 cggcgcatcc ttatttgagt agtgtggaaa aagcctgctt tatgagccac gtcgtattgt
     1081 ggaagcaggc gttggacgaa ggcgtaccgt atgttgccgt atttgaggat gatgtcttac
     1141 tcggcgaagg ggcggagaaa ttccttgacg aagacggctg gctaaaagag cggtttgacc
     1201 cggattccgc ctttatcgtc cgtttggaaa cgatgtttgc gaaagttatt gtcagaccgg
     1261 ataaagtcct gaattatgag aaccggtcat ttcctttgct ggagagcgaa cattggggga
     1321 cggctggcta tatcatttcg cgtgaggcga tgcggttttt cttggaaagg tttgccgttt
     1381 tgccggcaga gtggattaaa gcggtagatt ggatgatgtt tacttatttc tttgataagg
     1441 aggggatgcc tgtttatcag gttaatcccg ccttatgtac ccaagaattg cattatgcca
     1501 agttcctcag taaaaacagt atgttgggta gcgatttgga aaaagatagg gaacaagaaa
     1561 gaagacaccg ccgttcgttg aaggtaatgt ttgacttgaa gcgtgctttg ggtaaattcg
     1621 gtagagaaaa gaagaaaaga atggagcgtc aaaggcaggc ggagcttgag aaagcttacg
     1681 gcaggcgggt catatcgttc aaatagtttg tgtaaaatat agcggattaa aattagaaat
     1741 ggacacactg tcattcccgc gcaggcggga atccagtccg ttgagtttca gttatttccg
     1801 ataaatcacc gcaacgttaa atttctagat tcccgctttt gcgggaatga cggcggggga
     1861 tttctgtttt tccgacaaat tcctaaaact taaaattccg ttattcccgc gaaaacagaa
     1921 aaccaaa
//
LOCUS       DAWHRS010000219         1872 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWHRS010000219 DAWHRS010000000
VERSION     DAWHRS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.219"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..398)
                     /gene="mafB"
                     /locus_tag="WIB82_002035"
     CDS             complement(<1..398)
                     /gene="mafB"
                     /locus_tag="WIB82_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ3843279.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDNHDSRSTSDFSGGVDGGFTVYQLHR"
     gene            complement(438..563)
                     /locus_tag="WIB82_002036"
                     /pseudo
     CDS             complement(438..563)
                     /locus_tag="WIB82_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            complement(892..1302)
                     /locus_tag="WIB82_002037"
     CDS             complement(892..1302)
                     /locus_tag="WIB82_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843280.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIKGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(1306..>1872)
                     /locus_tag="WIB82_002038"
     CDS             complement(1306..>1872)
                     /locus_tag="WIB82_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ3843281.1"
                     /translation="AAGTVWRGKKVELNPTKWDWVKNTGYKKPAARPMQTLDGEMAGG
                     NKPAKQATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAITDGKYITDPLSMVRHK
                     PDASSLDKIDSKSLFKSDVDAEKATLDAARYADKHNLWDSQNKAKVPTNDIVGYTDGK
                     PTHIINIYRRKPNKDGFRPVHGTPGKER"
BASE COUNT          520 a          379 c          344 g          629 t
ORIGIN      
        1 cgatgaagtt ggtaaacagt aaaaccaccg tctacaccgc cgctgaaatc agaagtgctt
       61 cttgaatcat gattatcgaa cggactgtgc acttcatgtc catgtcctga aaaatgggtt
      121 tcatagccga taacaccttc aaatcctgtc cgttcgtgtg taataggcag tatggggccg
      181 accgcagttg catcaaatgt ttggacggcg taaacccgat ttttaacact gccgcgacca
      241 tcgccgaaca gatggtattt gccacccggt tcgtagtgtt ttgcctgcat atcatcgcgc
      301 aaacgggcat ccaaaccgtt cgcatgacta atggggattt gcagcaacga tattgctgct
      361 gcaaacagca tcatgaattt ttgaataggc aaattcattt tgttttcctt ggtagcaagc
      421 ggttatggta gtgtgaatca aggttgtcct tgtctatgtt gtcgcactgc ttcatcgctg
      481 tatccatacc cctcatgact gttatcagtc tctacggatg gggcggagtt acccgtatga
      541 ttgccgtatg gtcggatact tgtatccgac aaaaatattt gacgcgtcta tcgtttccga
      601 aatgccgctg ttgaaaaacg tcggattcga gaatccgacc tacggttgct gaacaatgtt
      661 ttgtagaact tgctcaaaat ccatgtttcg ccattttcct ttatatatac ctaatgcagc
      721 acgggttacg atttcgttta agtcataaat agccatttac gtcctctgct aattttcagc
      781 gagtagattg ggttaccaaa cccaacaaaa aagcagcctg aaaaatttca ggatgcttgt
      841 tttatgtttt ttatcagtga attaggtttt aatccagcct gcgcttgcta gttaaacagt
      901 atttacataa aaatcttgca ctgaacaaac aagcgtatca tgttcatcat gaaatgatgg
      961 gcataacgtt aatctacaaa tgtttacatt taaagatatt ttttcagttt caatattttt
     1021 aatttgtaag gttaatacat gaggatattt gtgatatagc tgatttttta attgttcgtt
     1081 gtctaatata gcaaaaggta attttgcaca caaataacca ataactttgt ttttatgaaa
     1141 aaaaactatc caattttcaa ttccttcatc ccaatcaaaa gtaaaatccg ttccttttat
     1201 gataaattta gcacaagata ttaaatctaa cttaacccaa gtggatttat acagactcaa
     1261 tgctgatgaa gttataatat tatctaaatt ataaattttc ataaatcaac gttccttacc
     1321 tggtgttcca tgcacaggtc taaacccatc tttattcggc tttctacgat aaatgttaat
     1381 tatatgtgtc ggttttccgt ctgtatagcc aactatgtcg ttcgttggaa cttttgcctt
     1441 attttgacta tcccataaat tatgtttatc tgcatatcta gcagcatcta atgtagcttt
     1501 ttccgcatca acatctgatt taaataaact tttgctatct atcttgtcta gactagatgc
     1561 atcaggtttg tgtcgtacca tactgagagg gtcagtaata tatttaccat ctgtaatagc
     1621 tgatccacca ttataggtag tatgcgtata atttttttgt ggaacgagac tattttgcaa
     1681 agattgttgt gtagaaacgc cagctttgcc attaggagta gcttgcttcg ctggcttatt
     1741 cccaccggcc atttccccat ctaaagtctg catagggcgg gcagcaggtt ttttataacc
     1801 ggtattttta acccaatccc atttagtcgg gttaagttct acttttttac ctctccaaac
     1861 cgtacctgcg gc
//
LOCUS       DAWHRS010000220         1848 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWHRS010000220 DAWHRS010000000
VERSION     DAWHRS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.220"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            208..1680
                     /gene="pyk"
                     /locus_tag="WIB82_002039"
     CDS             208..1680
                     /gene="pyk"
                     /locus_tag="WIB82_002039"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ3843282.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT          477 a          534 c          502 g          335 t
ORIGIN      
        1 aaataaattt agcctaagtc ataaaaccgt gtaattttgc gccaaaaaag gcggtttgca
       61 ggtaaaatat caaactatta atttaaatca tatttttaca gaatattccg ccgcgttcat
      121 caaatggaca tcaaaccggt tccaaatttc tgtaattttg taacaaaata ccgcaaaaca
      181 cccgattgag accaaaagga ctttcatatg aaccagacaa gccgcgatct gacccgcatc
      241 agccacaaca ctaaaatcgt cgccaccctt gggccgggca gcaacaacgt cgaactgttg
      301 gaagacatga tccgcgtcgg cggtctgaac gtcgtccgct tcaacttcag ccacggcacg
      361 cccgaattcc atcaggaaaa cgcccgcatt gtgcgcgagg cggcaaaacg cgccggacag
      421 gaaatcgcca tcattgccga cctgcaaggg ccgaaaatcc gcgtgggcaa aatcgccggc
      481 ggcggcatcg aattgaacaa aggcgaaacg ctggtactgg atgccgcgct cgaaggcgaa
      541 ggcacgcgcg aggcggtcgg tttggactac cgcgacctgc ccgacgacgt taccgcaggc
      601 gacgtattgt ggctggacga cggcctgctg accctgaccg tggaatccgt cgaaggcagc
      661 aggattatca caagggtgga aaacagccac gtcctgaaaa gcaacaaggg catcaacaaa
      721 cgcggcggcg gtctatctgc aggcgcgttg accgaaaaag acttccgcga cctgaaaacc
      781 gcgattgcca tcggttgcga ctacctcgcc atcagctttg tgaaatccgc cgaagatttg
      841 catatcgcgc gcgccaaagt cgaagaagaa atgaagggca gcactgccgt gcgccccggt
      901 ttggtttcca aaatcgaacg cgtagaagcg attgaaaact tggacgaaat catcctcgcc
      961 ggcgacggca ttatggttgc gcgcggcgac ttggcggtcg aagtcggaca cgccgccgtc
     1021 cccgccctgc aaaaacggat gatccgccgc gcccgcgagt tgcgccgctt cagcattacg
     1081 gcgacgcaaa tgatggaatc gatgattacc aaccccgtac cgacccgcgc ggaagtcagc
     1141 gatgtggcaa acgcggtatt ggacggtacc gatgcggtga tgtgttccgc cgaaaccgcc
     1201 gtcggcgcgt atccgtttga aaccgtcagc caaatggcga ttatctgcgc ggctgcggaa
     1261 aaagagcagg attcgctcaa cggcgttgcc gaacaggtcg agtatcccga agcggtcagc
     1321 accaacctgg cggttgccgg cggtgcggtc agcgtggcgc gcgcggttca cgccaaagcc
     1381 atcgtcgccc tgaccgaaag cggttcgacc gcctttgaaa tcagccgcca caacatcacc
     1441 ctgccgattt tcgcgctgac cccgagcgtt tccgcccaac gccgtatggc gatgtaccgg
     1501 ggcgtgcgcc cgctgatttt ggcaaccagt accgaccacg acacggcgtt aaacgaagtc
     1561 gaaacgatgc tggtggaaca caacatcctg cattccggcg accaatacat cattaccagc
     1621 ggttcgcaaa tgcgcgaatc cggttcgacc aacacactgg aagtgctgcg cgtcaaataa
     1681 tccgccctga gtggaaaatg ccgtctgaag ccgatgcccg aggcttcaga cggcattttt
     1741 tgcggcggcg cggcggttcg ggcaaactct gaaaaaattg tgcaaatccg gcaacatcgg
     1801 ataaaatcga gtacctatac taaagcgaaa caaggcattt ccgactgc
//
LOCUS       DAWHRS010000221         1804 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWHRS010000221 DAWHRS010000000
VERSION     DAWHRS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.221"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..79)
                     /locus_tag="WIB82_002040"
     CDS             complement(<1..79)
                     /locus_tag="WIB82_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ3843283.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WIB82_002041"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WIB82_002041"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ3843284.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMVGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
BASE COUNT          463 a          585 c          405 g          351 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccaaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatat agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgcccaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtcttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgcc ggacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaaccat ggtcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccagct ccaagcccgc accgccaaca
     1501 accgcgtcgt caactttacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa acccttacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgatgg
     1681 cgcggccagg cggcttaatt tgccgcatcc cgatccgaca accacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gtta
//
LOCUS       DAWHRS010000222         1796 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWHRS010000222 DAWHRS010000000
VERSION     DAWHRS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.222"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..334)
                     /locus_tag="WIB82_002042"
     CDS             complement(<1..334)
                     /locus_tag="WIB82_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3843285.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNRL"
     gene            complement(396..1067)
                     /locus_tag="WIB82_002043"
     CDS             complement(396..1067)
                     /locus_tag="WIB82_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3843286.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          426 a          390 c          460 g          520 t
ORIGIN      
        1 ccagccggtt gcggtagcgg cataaggtgc tgtaatcggg gatgctcagt tcgtcaaaac
       61 ggcaaaacag gttgaaatcg atgcgggtaa tgaggctgtg ttcgagttcg ggatcggaga
      121 ggctgtgcca ttgtccgagc aggacggctt tgaacatgga cagcagggga taggcgggac
      181 ggccgcggtg gtctctaagg taacgggttt tttgacggtt caggtactgc tcgatcggct
      241 gccaatcaat cacctggtcc aacttcaata gcgggaaacg gtcgatgtgt ctggcaatca
      301 tggcttgggc ggtttgctgg aagaaggtgc tcatggaaaa tcccctaaat gtcttggtgg
      361 gaatttaagg ggttttgggg aattttgcaa aggtctcagt ctatgcccga tatacaattt
      421 tgatacacaa acttggaaat atcggtatcg tcgccggagc gatagaatgc ggacagtttt
      481 tcattgaatt gcggcatctg catttcggag atttccaaga tgccgcagcc ccccgccatc
      541 atcagaccgt tggcaaataa ggttgccgtt cgtttgttgc cgtcccaaaa aacctgctgc
      601 cgcatacaat aaagcataaa acggacggct gcctccgtcg tcgaaccgct ttgcagtccg
      661 atattttgta acacgcgggc cacttcaatt tccttcactg gattcggggc atgacgggaa
      721 ccgtccaata gcgttacgcc gaccgaaccg gtacggaaat caccgggtgc caaagaatcg
      781 tccttggcaa caatgttgtt gattttttta aggagtgaaa tatcgaccgg ttcgccgttt
      841 gaaatatgcg aaatcacata ttgataggca cgtttcaggt tcaagatggt ttggatgtct
      901 tccagtgacg cggaggctac atttttgcca tggataattt gttcggtctg caacaaggtc
      961 gtctgacaat tctcaaaacg gctgaggttg tgaatttggg caaccaatac tttctttgcc
     1021 aaaaaaatat tttctttcaa actcaacttg tatttgtcgg gaaacacgat aattccttaa
     1081 ataattaaag cagtacgtta aatcatcatc ttatcatcac tttcggggtt tatgttgcgt
     1141 gcgtccgttt tctaatacgg tttcccatcc cgcgaaacga ggcggaggcc tgtgcaaaaa
     1201 acctgttcac gaaattttcc gcattaacga tacgctgaaa atgcgctaaa aatgactgtg
     1261 tttgaatatc ggttgatttc atccgtttgt gtaacctcgc gccggttttg tcatgatttt
     1321 gcattatagt gaattaaatt taaaccagta cagcgttgcc tcgccttacc gtactatctg
     1381 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta attcactata tttttgtcat
     1441 gcggatattt cacgggatga caaaacgggc gcaaaaaagc ccgattggaa aatccgaatc
     1501 gggctttttt gtgatgttct gttcgatttg cgtactaact gccggccgga gcgtccgcgc
     1561 ccggtttggc aggtcggatt ctcgaatccg acggctattt gagatggcag gcttttgtcg
     1621 gatacaagta tccgacctac ggcttgcttt tcgtcattcc cgcgaaagcg ggaatctaga
     1681 atctcggact ttcagataat ctttgaatat tgctgttgtt ctaaggtcta gattcccgcg
     1741 ttcgcgggaa tgacggttca gttgctacgg ttattgtcag gtttcggtta tgttgg
//
LOCUS       DAWHRS010000223         1783 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWHRS010000223 DAWHRS010000000
VERSION     DAWHRS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.223"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(99..422)
                     /locus_tag="WIB82_002044"
     CDS             complement(99..422)
                     /locus_tag="WIB82_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ3843287.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(410..649)
                     /locus_tag="WIB82_002045"
     CDS             complement(410..649)
                     /locus_tag="WIB82_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ3843288.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(772..1569)
                     /gene="truA"
                     /locus_tag="WIB82_002046"
     CDS             complement(772..1569)
                     /gene="truA"
                     /locus_tag="WIB82_002046"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ3843289.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALENALAQI
                     AGEAVSTIVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGPMRRAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          389 a          425 c          520 g          449 t
ORIGIN      
        1 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta
       61 atccactata atgaacacaa tccattcaga ctattcaatc aggcaaacat ctcctgcaat
      121 actgcaaaca gtttttcagc cgtactgttg tctaaatttc caagatgttt gaccaatccg
      181 gctttatcca cagccctaat ctgttcgggc aaaagcaaac cgtctttatc ctgaaagcgg
      241 acattgacgc ggaacggggc aggacggctt ccgctcgtca tgggaacgat cagcacagtc
      301 ttgagatagt tgtgtatttc aggaggagag actacgacac aaggacgtgt ctttttgatt
      361 tcgcttccta cggtcgggtc taaggagacc agatagattc cgccgcgtac taccatatcc
      421 attctttatc cgcttcgttt tcaatttcgg aaaaaaaatg ctcctgctcg gtttcgacaa
      481 gcattgcggc agcttctgcc catcccctgc gaacggtagg acagcttaaa atgatattgc
      541 ccttttcaac tgtaacagcc aagctgtcta ctgcccctat ttgacccaat aatgatttgg
      601 gcagaatcac gccttgcgag tttcccattt tgcgtatgtt gagaatcata tacgtaccct
      661 atttaccagt ttatgtaata acaatgttat taccttgatg aggtagtgtc aatattgaaa
      721 aagattgcct gacagtttgt ccgatttcaa aatctccgcg acaagaatgt tttaaagcca
      781 ttcggggatt tgggggcgga tgatgccgta tgcctcggga tagtcgacgc cggtcaggta
      841 aagtccgtcg ggcatgaagg tcggcggggc tttgaggcgg ctgcgttctt gaatcagtgc
      901 ggcgaagcct tcgacgctga gtctgccgct gccgacatag acgagcgcgc ccatgatgtt
      961 gcgtaccatg tggtgcaaaa aggcgttgcc gtgcaaatcg aggcggacga gtcctgcgct
     1021 ttgggtaagg tcggcgcggt agatggtttt gacgggggat tttgcctggc attcggcggc
     1081 gcggaagctg gagaagtctt gttcgccgat caataaggcg gcagcccggc gcatcggccc
     1141 gatgtcgagt ttgaggtgtg tccagcctgc cctgtttttg agcagggggg aacggacggg
     1201 ggcggattcg agcaggtagc ggtagtgccg tccgtatgcg tcgaagcggg cgtgaaattc
     1261 gggggcgacc tgtcgggcgt gcaaaacggc aatgccttcg ggcaggtggg catttacgcc
     1321 gcgcacccat gcctgttggg gacgggcggc agttgtgtcg aagtggacga cttgggcggt
     1381 ggcatgcacg ccggtgtcgg tcctgccggc aacaatggtg gaaaccgctt cccctgctat
     1441 ttgggcaagt gcgttttcca atgccgcctg aacggtcggt acgccgtcag cctgtttctg
     1501 ccagccgtaa aagcggctgc cgtcatagga tagggttatt gcccagcgtt gtttttgtgc
     1561 ggtatccatc ggatttggga ttcggataaa tgttcagact gcattgtatc gcagattttg
     1621 cagggaaacg gcaaacgccc ggggcgagcg gcgttgtttg gggagttggg gggggtggat
     1681 gcagttgcta cgaatcgcta tcctgtgaat tcaccctgtc aggagtgccc gaatcgtcat
     1741 tcccgcgcag gcgggaatct aggacgtaaa atctaaagaa acc
//
LOCUS       DAWHRS010000224         1720 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWHRS010000224 DAWHRS010000000
VERSION     DAWHRS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.224"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(55..834)
                     /locus_tag="WIB82_002047"
     CDS             complement(55..834)
                     /locus_tag="WIB82_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ3843290.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            1337..1642
                     /locus_tag="WIB82_002048"
     CDS             1337..1642
                     /locus_tag="WIB82_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ3843291.1"
                     /translation="METWVQNYTAIGGSLYLTAAAALLPIVFFFAALTVLKLKGYQAG
                     LYTLLIALAVAVFGFGMPAVWRFLPCRSSRTDPTPLRHAGFDRALQNRQIHPHRFGL"
BASE COUNT          433 a          518 c          351 g          418 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ctagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcgaccaaa acattcaatg ccgacctgac cgacgggcgc gaaacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttccg caatgcgctc
      781 ctccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg cagaaccctt ccgaaatata gtggattaac aaaaatcagg
      901 acaaggtgac gaagccgcag acagtacaaa tagtacagaa ccgattcact tggtgcttca
      961 gcaccttaaa gaatcgttct ctttgagcta aggcgaggca acgccgtact ggtttttgtt
     1021 catccactat acatcaaaca tcaaattgga ctgaccaatc agggcggatt ctaatgacac
     1081 gccgttcccg ccgtcaacgg catttacctc gcaccgcccc cgaaacacaa gaaaaaacta
     1141 caccaaacta caatttttgt tcatgcaaat atttgttttg acaggattta aacgaaagct
     1201 ccgattcaaa tctgccgaac cgcccaaaaa tatattgacc taaatattaa agtttcgtaa
     1261 agtaatgcaa cgttgcttta attggtttga ccactattgc cgacgattag aaaaatattt
     1321 tcggagatgt tcaattatgg aaacttgggt tcaaaactac acggcaatcg gcggcagcct
     1381 gtatctgact gccgccgccg cactcttacc catcgtcttt ttctttgccg cgctgaccgt
     1441 cctgaagctg aaaggctatc aggcggggct ttatacgctg ctgattgcgc ttgccgttgc
     1501 cgtattcggc ttcgggatgc cggcggtatg gcggtttctt ccctgccgca gcagccgcac
     1561 tgacccaacg cccctacgcc acgctggatt tgaccgcgca ttacaaaatc ggcaaatcca
     1621 cccgcatcgg tttggacttt gaaaacgtgt tcaacaaacg ctaccgccct atgcccgaca
     1681 ttcacgttta cggcacgccg cgcagcctga ccgcaaccgt
//
LOCUS       DAWHRS010000225         1716 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWHRS010000225 DAWHRS010000000
VERSION     DAWHRS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.225"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            342..1646
                     /locus_tag="WIB82_002049"
     CDS             342..1646
                     /locus_tag="WIB82_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ3843292.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGVVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          350 a          431 c          509 g          426 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattg gcggcacatc ggcaaaatcc
      241 ccgcgccgcc ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt
      301 tccgaaagac gggctcaacc attaaggaaa ctttagtcaa aatgaaaaaa cacatatggg
      361 cggcatcttt gctgccggca tccctatcgg cagaaccttt aaactggtgg aagccttatt
      421 ccgccgtcaa ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga
      481 tgacgcttcc cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct
      541 cgacgatgat gcacagcttt tccatcgcga cattggtggg catcctttgg gtcgccgtcg
      601 gctattcttt agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt
      661 taagcgggat gcagatagac gctaccgcac agatgctgac cgtgtcgccc aatgcgccga
      721 ctgttcccga accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca
      781 ttattaccgg cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca
      841 tatggttttt attggtttat gtgccgggcg cgcattgggt gtggggcggc ggctttatga
      901 gcaagggcgg cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg
      961 cgggactcgt cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc
     1021 ctccgcacaa tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg
     1081 gctttaacgc cggatcggcg cttgcggcag acgcggcggc gggtatggcg atggcggtaa
     1141 cgcaggtgtc ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac
     1201 acaaaccttc cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc
     1261 ctgccgccgg ctttaccggc ccgtcgggcg cggccgccat cggtatattg actgccgccg
     1321 cgtgctttgt gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg
     1381 ctttcggcat acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg
     1441 acaaccgcat tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag
     1501 acggggtcgt tatggcggca tacagcgggc taatgagttg ggcgattttg aaggtcgtgg
     1561 ggaaaatctg cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga
     1621 atatccacgg cgaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg
     1681 tttcagacgg catttttcac gtttgccgcc gatgga
//
LOCUS       DAWHRS010000226         1716 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWHRS010000226 DAWHRS010000000
VERSION     DAWHRS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.226"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1686
                     /locus_tag="WIB82_002050"
     CDS             <1..1686
                     /locus_tag="WIB82_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3843293.1"
                     /translation="FFDKDATVFDTVAEGLGEIRDLLRRYHHVSHELENGSSEALLKE
                     LNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGGQKKRVALAQAWVQKPDV
                     LLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDNIATRIVELDRGILRSYP
                     GSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEARRTRNEGRVRRLEELRR
                     QRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVIMDKFSAILQRGDKIGLI
                     GPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRSALNENDTVFYTLGQGND
                     YVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLLLAKLFTRPANILVLDEP
                     TNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSIVFEGQGRLKEYIGGYQD
                     YIDAKSREDKIQTASAPKASDAEPAKEKPKANRTVKLSYKEQRELDALPDEIAALEAE
                     QAEINTQLSYPEIFKDYEKAGALQNRAEEIEMLLLEKLERWELLETKRNGNAV"
BASE COUNT          488 a          442 c          430 g          356 t
ORIGIN      
        1 ttttttgata aggacgcaac cgtatttgat accgttgccg aaggtttggg cgaaattcgt
       61 gatttattgc gccgttatca tcatgtcagc catgagttgg aaaatggttc gagtgaggct
      121 ttgttgaaag aactcaacga attgcaactt gaaatcgaag cgaaggacgg ctggaaactg
      181 gatgcggcag tcaagcagac tttgggggaa ctcggtttgc cggaaaatga aaaaatcggc
      241 aacctttccg gcggtcagaa aaagcgcgtc gccttggctc aggcttgggt gcaaaagccc
      301 gacgtattgc tgctggacga gccgaccaac catttggata tcgacgcgat tatttggctg
      361 gaaaatctgc tcaaagcgtt tgaaggcagc ttggttgtga ttacccacga ccgccgtttt
      421 ttggacaata tcgccacgcg gattgtcgaa ctcgatcgcg gtattttgcg ttcctatccc
      481 ggctcgttct ctaaatacag cgagaaaaaa gcgcaagagt tggcagtcga agcggaacac
      541 aaccgcctct ttgacaaatt ccacgcacag gaagaagcat ggatacgcaa aggcatcgaa
      601 gcgcgccgta cccgcaacga aggccgcgtg cgccgtttgg aagagctgcg ccgccagcgt
      661 gcagaacgcc gcaacgtaca gggacaagtc aacttcaagc tcgatagcgg tgagaaaagt
      721 ggcaaaatca tcgccgaatt ggaacacgct tcgtttgcct atggcggcaa agtcattatg
      781 gacaaattct ccgctatctt gcagcgcggc gacaaaatcg gcctaatcgg ccccaacggc
      841 atcggcaaaa ccaccttcct taagctgatt ttgggcgaat tgcagccgac ctacggcaga
      901 atccgtatcg gcagtaagca ggaagtcgcc tatttcgacc agttccgcag cgcgttgaat
      961 gaaaacgaca ccgtgtttta caccctcgga cagggaaacg attacgttga agtcggcggt
     1021 aagaaaaaac acgtcatgag ctatttggaa gatttcctgt tccatccagc ccgtgcgcaa
     1081 agccctgttt catcgctctc cggcggagaa cgcaaccgcc ttttgctggc aaaactcttt
     1141 acccgtcctg ccaatatcct ggtcttggac gaaccgacca acgacttaga catcgacacc
     1201 caagagctgc tcgaagacct gttgcgcgat tatcaaggca cagtattcct tgtctcgcac
     1261 gaccgtatgt tccttgataa cgtaattacc caaagcattg ttttcgaagg acaaggccgt
     1321 ctgaaagaat acatcggcgg ctatcaggac tatatcgacg caaaatcgcg ggaagataaa
     1381 attcagacgg catccgcacc caaagcatcc gatgccgaac ccgccaaaga aaaacccaaa
     1441 gccaaccgca cggtcaaact gtcctacaaa gagcagcgcg aactcgatgc cctgcccgac
     1501 gaaatcgccg ctttggaagc cgagcaggct gaaatcaata cccaactttc ctatcctgaa
     1561 attttcaaag attacgaaaa agcaggtgca ttgcaaaacc gcgccgaaga aatcgaaatg
     1621 ctgcttttgg aaaagctgga acgctgggag ctgctggaaa ccaaacggaa cggcaatgcc
     1681 gtctgaaatc aaacaggaag aaaaaaatgt tcaaac
//
LOCUS       DAWHRS010000227         1685 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWHRS010000227 DAWHRS010000000
VERSION     DAWHRS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.227"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(195..1361)
                     /locus_tag="WIB82_002051"
     CDS             complement(195..1361)
                     /locus_tag="WIB82_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ3843294.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEQP
                     SRTQGQTSNQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGG
                     GATRWGNRESFVGLAGEFGTLRAGRVANQFDDASKAIDPWDSNNVVASQLGIFKRHDD
                     MPVSVRYDSPDFSGFSGSVQFVPAQNSKSAYKPAYVDEKKMVHAAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFNLFLLGRIGDDDEAKGTDPLKNHQVHRLTGG
                     YEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGK
                     KGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          439 a          431 c          384 g          431 t
ORIGIN      
        1 tatatgtgca aaccaagcca aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaag gcgagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgataaagtc gaaaccatgg gcatagctga tgcgtggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt gtcgccattt
      481 tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcctc atagccgccc
      541 gtcaggcggt gtacctgatg gtttttcaag ggatcggtac ctttggcttc atcatcatcg
      601 ccgatgcgcc caagcaagaa caaattaaaa gcatcacggc ccacattggc gtgtttcgca
      661 tatttaaagg catagttccc ggcaaaaccg ccatttttgt aattcagacc ggcataatac
      721 acatccgatc cgggcttgcc gacaacagcc gcatgaacca ttttcttctc atccacataa
      781 gccggcttat aggcggactt gctgttttga gccggaacga attggacgct gccgctgaaa
      841 ccggaaaagt ccggagagtc gtagcgtacg gaaaccggca tatcgtcgtg gcgtttgaaa
      901 atacccaatt gcgaagccac aacattattg ctgtcccaag gatcaatggc tttgctggca
      961 tcgtcaaact gattcgcaac gcggccggcg cgcagcgtgc cgaattcgcc tgccaagccg
     1021 acaaaggatt ccctgttgcc ccaacgggtc gcgccgccgc cggcaacgga tacgtcttgc
     1081 tcaagctgcc aaacagcctt cagaccttcg cccaaatcct cgctcccctt aaagccgata
     1141 aacgagccga aatcactgat tttcgtcctg atgcggcttt tggccttagt aacttttacc
     1201 tgattgctcg tttgaccttg agttcttgag ggttgttcag tcaattgcag ctggtagttc
     1261 ctgccttcca cgccggcttt gatttcgccg tacaggctga catcggcaac ggccgcaagc
     1321 ggcagtgcgg acaatacgag ggcggtaagt ttttttcgca tatcggcttc cttttgtaaa
     1381 tttgataaaa acctaaaaac atcgggcaaa cacccgatac gtcttcaatt ataccccccc
     1441 cccgcaaaaa accatttttc agaacaaata tctgataaat gccgcaacct ttattttaaa
     1501 aaatgattat attttgatat aaaacaatag cttatttttt caaaaaacgt tgtgtttcta
     1561 caacacaatt caagcgcaga cctcgtgcgg gccgatgcgc tactgcctag aagcagtccc
     1621 ggctttttaa aacgccataa aaaaacacac gcggcacttt atagtggatt aacaaaaacc
     1681 agtac
//
LOCUS       DAWHRS010000228         1645 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWHRS010000228 DAWHRS010000000
VERSION     DAWHRS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.228"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..244)
                     /locus_tag="WIB82_002052"
     CDS             complement(<1..244)
                     /locus_tag="WIB82_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3843295.1"
                     /translation="MKITHCKLKKKVQKELLRFFALEITARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGR"
     gene            851..>1645
                     /locus_tag="WIB82_002053"
     CDS             851..>1645
                     /locus_tag="WIB82_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ3843296.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNYHRYGENDY
                     STSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNN"
BASE COUNT          533 a          301 c          332 g          479 t
ORIGIN      
        1 gtctgccttt acgccgtccg ccgaaatcgc tttcgtccgg ctcgacaggg ccctcaaaaa
       61 cctcatcggc agccaaggcc aaatgatggt tgataaccgt gcggatttta cggtagaaca
      121 gtgctgccga attgggatgg atacccaaaa tatcggcggc agaacgggcg gtaatttcca
      181 gcgcaaaaaa acggagcagt tctttctgta cttttttctt taatttgcag tgtgttatct
      241 tcatatttcg agggtaacat atctgctaat ctagtacagc cccaaaaata taccaaaaac
      301 agcaaaacaa attgtaagga taggtatagg ctttgtaaag gtaaattgtg aaaaaagcag
      361 ttttttaaac gaatgaaacg gcttcgggct gaaatatatg ctgatgccct gtccttcccg
      421 tatatcttgt gtgttgtcaa agtgcaggct gctttgaaat tggcattaca gtcgatgaac
      481 caccattttg ctttatttca gcgggcttga gatgtgtata agaatattgt tttgaataaa
      541 tttaaagaaa atgataatcg ttattgacga tttttaaagg aaagcgtaga gtgccaattc
      601 tatgaagcaa tacggtaagt aacaatgaaa atatctactg cttgggtata gagcatattt
      661 cacaacccgt aactattctt gcggaaacag agaaaaaagt ttctcttcta tcttggataa
      721 atatatttac cctcagttta gttaagtatt ggaatttata cctaagtagt aaaagttagt
      781 aaattatttt taactaaaga gttagtatct accataatat attctttaac taatttctaa
      841 gcttgaaatt atgagaccat atgctactac catttatcaa ctttttattt tgtttattgg
      901 gagtgttttt actatgacct catgtgaacc tgtgaatgaa aagacagatc aaaaagcagt
      961 aagtgcgcaa caggctaaag aacaaaccag tttcaacaat cccgagccaa tgacaggatt
     1021 tgaacatacg gttacatttg attttcaggg caccaaaatg gttatcccct atggctatct
     1081 tgcacggtat acgcaagaca atgccacaaa atggctttcc gacacgcccg ggcaggatgc
     1141 ttactccatt aatttgatag agattagcgt ctattacaaa aaaaccgacc aaggctgggt
     1201 tcttgagcca tacaaccagc aaaacaaagc acactttatc caatttctac gcgacggttt
     1261 ggatagcgtg gacgatattg ttatccgaaa agatgcgtgt agtttaagca cgactatggg
     1321 agaaagattg cttacttacg gggttaaaaa aatgccatct gcctatcctg aatatgaagc
     1381 ttatgaagat aaaagacata ttcctgaaaa tccatatttt catgaatttt actatattaa
     1441 aaaaggagaa aatccggcga ttattactca tcggaactat cataggtatg gagagaacga
     1501 ttacagcact agcgtaggtt cctgtattaa cggtttcacg gtacggtatt acccgtttat
     1561 tcgggaaaag cagcagctca cacagcagga gttggtaggt tatcaccaac aagtagagca
     1621 attggtacag agttttgtaa acaat
//
LOCUS       DAWHRS010000229         1622 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWHRS010000229 DAWHRS010000000
VERSION     DAWHRS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.229"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1012
                     /gene="argH"
                     /locus_tag="WIB82_002054"
     CDS             <1..1012
                     /gene="argH"
                     /locus_tag="WIB82_002054"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ3843297.1"
                     /translation="RDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQVAQPVSF
                     GHHMLAYVEMLGRDNERMADCRCRVNRMPLGAAALAGTTYPIQREITAELLGFEQICQ
                     NSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCTGSSIMP
                     QKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTADTLIDTL
                     RIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQAVRHADQ
                     AGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQVKRWREM
                     LA"
     gene            1070..1453
                     /locus_tag="WIB82_002055"
     CDS             1070..1453
                     /locus_tag="WIB82_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ3843298.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGSILQFVCNWGFPAAEKTCSVLKKIGAVHSAMLIHRAADALDKEIRRLQS
                     EGKNLKEMWDITSRQQNRLTAEQSG"
BASE COUNT          412 a          471 c          420 g          319 t
ORIGIN      
        1 gcgcgaccag attaccgtta tacaaagcct gattcaaagc cttcagacgg cattgctgga
       61 tttggcggaa caaaacgccg aaaccgtcat gccaggcttt acccacctgc aagtcgccca
      121 gcccgtcagc ttcggacacc atatgctcgc ctacgtcgaa atgctcggac gcgataacga
      181 acggatggcg gactgccgct gccgcgtcaa ccgtatgccg ctcggcgcag ccgcccttgc
      241 cgggacgacc tacccgattc agcgcgaaat caccgccgag ctattgggct ttgaacaaat
      301 ctgccagaac tcgctcgatg ccgtatccga ccgcgatttc gccattgagt tcacagccgc
      361 cgcctcgctg gttatggttc acctgagccg cctgtctgaa gaattgattt tgtggatgag
      421 cccgcgtttc ggctttatcg acatcgccga ccgtttctgc acaggttcgt ccatcatgcc
      481 gcagaagaaa aaccccgacg tgcccgaact cgtgcgcggc aaatccggcc gcgtcatcgg
      541 acaccttatc ggtctgatta ccctgatgaa atcccaaccc ttggcgtaca acaaagacaa
      601 tcaggaagac aaagaaccct tgttcgacac cgccgacacg cttatcgaca cgttgcggat
      661 ttacgccgat atgatgcgcg gcgtaaccgt caaacccgac aatatgcgcg ccgccgtgat
      721 gcagggcttc gctaccgcca ccgacttggc ggattatctg gtcaaaaaag gcatgccttt
      781 ccgcgatgcc cacgaagtcg tcgcccaagc cgtgcgccac gccgaccaag cgggcgtcga
      841 tttgagcgaa ctgccgctcg aagtcttaca aggtttcagc gatttgattg ccgacgacgt
      901 ttacggcgtg ctgacacccg aaggcagctt aaacgcccgc aaccacttgg gcggtaccgc
      961 gccggaacaa gtccgcttcc aagtgaaacg ctggcgggaa atgttggctt aacccccaaa
     1021 tgccgtctga agaaatgttc agacggcatt tttaaaaggc aagaacacga tgaccgatac
     1081 ggatacccaa gccgaccgct tcgaacagat gatgtggcag gcggtggaca aactttttga
     1141 acagcatgac ggcaaactcg aaagcatgga cgggcgggaa caggagctgg ttttgatttg
     1201 gcggacagaa gccgatatcg gtaacggcag catactgcaa tttgtctgca actggggttt
     1261 ccccgccgcc gaaaagactt gttccgttct gaaaaaaatc ggcgcggtac acagcgcaat
     1321 gctgatccat cgggcggcag acgcattgga caaagaaatc cgccgcctcc aatcggaagg
     1381 taaaaacctg aaagaaatgt gggatataac atctcgacaa caaaaccgcc tcactgctga
     1441 acagtctgga tgaacaatat tggcaagacc ccgacaaact gtatctgctg ggatggcaat
     1501 actattccag caaccctgtt cagacggtgg cggattcgca tttgaagtgc aactttccct
     1561 aacagaaaaa ggccagtatg cggtagcata cggcctttcc tgcaagaaag attgccatga
     1621 gc
//
LOCUS       DAWHRS010000230         1605 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWHRS010000230 DAWHRS010000000
VERSION     DAWHRS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.230"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            60..683
                     /gene="rsmG"
                     /locus_tag="WIB82_002056"
     CDS             60..683
                     /gene="rsmG"
                     /locus_tag="WIB82_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ3843299.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLVYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            782..1555
                     /locus_tag="WIB82_002057"
     CDS             782..1555
                     /locus_tag="WIB82_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ3843300.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVEEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
BASE COUNT          336 a          382 c          537 g          350 t
ORIGIN      
        1 ggtttcagac ggcatgtttt tccgctaaca ttacgcctga atatggacag gaagcagata
       61 tggaacgcaa agaacgcctg cgtgcaggca ttgccgcgat ggggctggat atttcggaaa
      121 cggcgcagga caggcttttg gtctatgtgg atttgttgaa aaagtggaac aaaacctaca
      181 atctgaccgc cctgcgcgac gaggaaaaaa tgattgtcca tcatcttttg gacagcctta
      241 cgctgctgcc ccatatcgag ggtgtgcaaa cgatgctgga tgtcggttcg ggcggcggtc
      301 agcccggcat tccggcggcg gtgtgccgtc cggatgtgca aataaccctt ttggatgcga
      361 atacgaagaa aacggctttt ttacagcagg cggttatcga gttggggttg gacaatgtgc
      421 gcgtggtatc cggacgcgtg gaggcggttt cggacgtgcg tgccgatgtg gttaccagcc
      481 gtgcgtttgc agaactggcg gattttgtgt cgtggacggt gcatctgttg aaagacggcg
      541 gctactgggc ggcgatgaag ggcgtgtatc cgcaggaaga aatcggccgc ctgccgcagg
      601 atgtgtgcgt tgaaaaagtc caaaggctcg acgtgccggg cttggatgcg gaacgccata
      661 tcgtcatcct gagcaagcgt tgagcgcact tcagacggca tgaatacctt ttttgtgcgg
      721 ataaaggtaa aattccgcac tgtttttctt ttttcaacat cagacgggac acgggcggga
      781 catgagtgcg aacatccttg ccatcgccaa tcagaagggc ggtgtgggca aaacgacgac
      841 gacggtaaat ttggcggctt cgctggcatc gcgcggcaaa cgcgtgctgg tggtcgattt
      901 ggatccgcag ggcaatgcga cgacgggcag cggcatcgac aaggcgggtt tgcagtccgg
      961 cgtttatcag gtcttattgg gcgatgcgga cgtgcagtcg gcggcggtac gcagcaaaga
     1021 gggcggatac gctgtgttgg gtgcgaaccg cgcgctggcc ggcgcggaaa tcgaactggt
     1081 gcaggaaatc gcccgggaag tgcgtttgaa aaacgcgctc aaggcagtgg aagaagatta
     1141 cgactttatc ctgatcgact gcccgccttc gctgacgctg ttgacgctta acgggctggt
     1201 ggcggcgggc ggcgtgattg tgccgatgtt gtgcgaatat tacgcgctgg aagggatttc
     1261 cgatttgatt gcgaccgtgc gcaaaatccg tcaggcggtc aatcccgatt tggacatcac
     1321 gggcatcgtg cgcacgatgt acgacagccg cagcaggctg gttgccgaag tcagcgaaca
     1381 gttgcgcagc catttcgggg atttgctttt tgaaaccgtc atcccgcgca atatccgcct
     1441 tgcggaagcg ccgagccacg gtatgccggt gatggcttac gacgcgcagg caaagggtac
     1501 caaggcgtat cttgccttgg cggacgagct ggcggcgagg gtgtcgggga aataggtcaa
     1561 tccaaatcgg gctgcccgtg cctttatgct gtttggccgg gtgcg
//
LOCUS       DAWHRS010000231         1595 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWHRS010000231 DAWHRS010000000
VERSION     DAWHRS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.231"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..284)
                     /locus_tag="WIB82_002058"
     CDS             complement(<1..284)
                     /locus_tag="WIB82_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ3843301.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNA"
     gene            612..1559
                     /locus_tag="WIB82_002059"
     CDS             612..1559
                     /locus_tag="WIB82_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3843302.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
BASE COUNT          412 a          420 c          417 g          346 t
ORIGIN      
        1 gcgtttttct cgacttgagc cgccagttcg cgggtcgggg tcaacaccaa agcacgcggg
       61 cctttgcccg gtttttcgct gcgtttggtc agtttttgca aagtcggtaa cagaaaggcg
      121 gcggttttgc cggagccggt ttgcgccgaa gccatgatgt cgcggccttc caaagcaaac
      181 ggaatggctt gcgcctgaat cggcgtcggg ctttcgtaac cctcgctgct gacggcggac
      241 aaaatgtttt tatcaaggtt caaatcggca aatttaatag acatggctat ccttggaaga
      301 caacaacgcc cgcgtccgaa aggttcagac ggcctgaagc agggaagggt aacgatacgg
      361 ggaatgtgaa gttacagggt tgtcgcaagc ggcttgcttg tggtttacat cgacggcaac
      421 ctgcacagat atgctttgca aaatcaaagc aacagaagaa acgcgcccgt aagggcggtt
      481 taggttggaa ggcgatggct tcgtatgaca agataagcga cggatggtat agggtttgcc
      541 cgaaacggtc aagcaatcgg gcgttaatta cagtaaaatt gccgttttcc actttattcc
      601 gcctgaaacc aatgaccgac atcacaccgt ttgccaaccg cttgggcaaa aacatcaaac
      661 accttatgaa atgggcgaaa cgcaacggca tcgaagcctg gcgcatttac gaccgcgaca
      721 tcccccaatt cccctttgcc gccgatgttt acggcgatcg aatccacctt caggaatacg
      781 ataccggctg gctgatgcgg cccgaagaat acgaagcgtg gcttgccgaa gtcttggaag
      841 ccgtcgcttt cgttaccggt tttgcgcccg aacaaatccg cctcaaacgc cgcgaacgcc
      901 aaaaaggttt gcagcagtac gagaaaaccg gcaaggcggg cgacgatttc gtcatcaccg
      961 aaaacggccg caagttttgg gtcaaccttg ataagtattt ggatacgggg ctttttttgg
     1021 atcatcgaaa cacgcgcaaa aaagtcggcg aaacggcggc gggcaaacgt tttttaaacc
     1081 tgttttccta caccggcagc ttcaccgtct atgccgccac cggcggcgcg gcatccagcg
     1141 aaaccgtcga tttgtccaac acatatcttg attgggcgaa acgcaatttt gaattgaacg
     1201 gcatcgatac ggaacgacac aaaatcgtcc gcgccgacgt gttccaatat cttcagacgg
     1261 catatgggga aggcaggcgg ttcgacctca ttgtgatgga cccgcccagt ttttccaaca
     1321 gcaagaaaat gtccgacatc ctcgacatcc agcgcgacca caaaaagctg attgacggcg
     1381 cggtgaagct gctcgcttca gacggcattt tgtacttttc caacaacctg cgcagcttcg
     1441 tcttagacga cttggtatcg gaacaatacg ccgtcaaaga catttccaaa caatccgttc
     1501 ccgaagattt ccgcaacaaa aaaatccata ggtgctggga aatccgccac aaatcttaag
     1561 ggtgcgaaaa agccgccgga atcttccggc ggctt
//
LOCUS       DAWHRS010000232         1595 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWHRS010000232 DAWHRS010000000
VERSION     DAWHRS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.232"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            62..1435
                     /locus_tag="WIB82_002060"
     CDS             62..1435
                     /locus_tag="WIB82_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ3843303.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGREIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          415 a          524 c          386 g          270 t
ORIGIN      
        1 ttccccgtcc tatcggtttc ccgtttcaga cgacataagg tctgaaagaa agactacaat
       61 tatgagtaat ccattttcct ctttaggttt gggtacggaa ctcgtttccg cactgaccgc
      121 gcaaggttac gaaaacccga cgcccatcca agccgccgcc attcccaaag cactcgccgg
      181 tcatgatttg ctagccgccg cgcaaaccgg cacaggcaaa accgccgcct ttatgctgcc
      241 cagtctggaa cgcctcaaac gttacgccac cgccagcacc tcgcccgcga tgcaccccgt
      301 gcgtatgctc gtcctcaccc ccacgcgcga acttgccgac caaatcgacc aaaacgtgca
      361 gggctacatc aaaaacctgc cgctgcgcca caccgtcttg ttcggcggta tgaatatgga
      421 caaacagacc gccgacctgc gtgccggccg cgaaatcgtc gtcgccaccg tcggacggct
      481 gctcgaccac gtgaaacaga aaaacatcca tttgaacaaa gtcgaaatcg tcgttttgga
      541 cgaagccgac cgtatgctgg atatgggttt tatcgacgac atccgcaaaa tcatgcagat
      601 gctgccccgc caacgccaaa ccctgctctt ttccgccacc ttctccgccc cgatacgcaa
      661 actggcgcaa gacttcatga acgcgcccga aaccgtcgaa gtcgccgcgc aaaacaccac
      721 caacgccaac gtcgagcagc acatcatcgc cgtcgatacc attcagaagc gcaacctgct
      781 cgaacggctg attgtcgatt tgcatatgaa ccaggtcatc gtgttctgca aaaccaaaca
      841 aagcgtcgac cgcgtaacgc gcgaactggt gcgccgcaac ctgtccgcac aggcgataca
      901 cggcgaccgt tcccaacaaa gccggctcga aacactcaac gccttcaaag acggcaacct
      961 gcgcgtcctc gtcgccaccg acatcgccgc gcgcgggctg gacattgccg aactgccctt
     1021 cgtcatcaat tacgaaatgc ccgcccagcc cgaagactac gtccaccgca tcgggcgcac
     1081 ggggcgcgcg ggcgcggacg gcgtggcgat ttccctgatg gacgaatccg aacagaaaat
     1141 gtttgaatcc attaaagagc tgaccggcaa caagctgctc atcgagcgca tcgagggctt
     1201 cgagccgcaa tggtgggaac agggcggcgc aaaaccggaa aaacccgaaa tgcgcgaacc
     1261 gagacaacgc aaccgctacg aatccgccaa agcgcaacgc gaaaaaaaca cccggccgga
     1321 aaatgcggca aacgatgcgg gcgcggcttg cggaaaaatt gccggacgca gccgccgaag
     1381 ccgccgggaa caccggacgt gcgccctgct ccaaccgcgt tacggcgtaa aataaccctg
     1441 aaaatcaaat gccgtctgaa catttcccgt ttcagacggc atttttcaaa ccagactaac
     1501 gcattgggcg caaccgccca taccggataa atttctgccg caaacagttt cagacggcat
     1561 ttgctgcctg tacaatatag tggattaaca aaaat
//
LOCUS       DAWHRS010000233         1551 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWHRS010000233 DAWHRS010000000
VERSION     DAWHRS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.233"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(874..>1551)
                     /locus_tag="WIB82_002061"
     CDS             complement(874..>1551)
                     /locus_tag="WIB82_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3843304.1"
                     /translation="NMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSA
                     EAPNAQPLPEVSPAENPANNPNPNENPGTSPNPEPDPDLNPDANPDTDGQPGTRPDSP
                     AVPGRTNGRDGKDGKDGKDGGLLCKFFPDILACDRLPESNPAEDLNLPSETVNVEFQK
                     SGIFQDSAQCPAPVTFTVTVLDSSRQFAFSFENACTIAERLRYMLLALAWAVAAFFCI
                     RTVSREV"
BASE COUNT          266 a          468 c          462 g          355 t
ORIGIN      
        1 cgcattcgcg gcgcggcggc gctgtgggga cacccccctc gcgcttatcg ccaatccccc
       61 cgcgcttctc ggctatttgc gacattcgtc gcaaagtgcg ctgcttcgcc tgcctttcgg
      121 ccaaagtttc cgaagctgaa cgctttgcgg gggactagtc ccccacaccc cctagtctca
      181 cttgcgacgc cgcgggggca tggggacggc gcaaaaggcg cgcgccttac cacctgccct
      241 tgcggcagag tgtatttctt tgtccgagcg gcaaggggta tccataaaga atcataaagg
      301 cgaaaaaacc ccgcctttac gattctttac ggacatcctc cccctgcctg aacaaaggtt
      361 actgaagccc ggcggtgctt cgcctgcctt ttaccatggc ttccgcccag accggcggta
      421 cttcgcctgc ctttcggccg tggcttccgc ctaagcctag cggtgcttcg ccctgccttt
      481 cggccgtggc ttccgcccaa ccggcggtgc ttcgcctgcc tgtagcctgg cttccgccta
      541 gctcggcggt gctgcgcctg cctgtagcct ggcttccgct cagaccggca gtgctgcgcc
      601 tgcctttcgg ccgtggcttc cgaagctgaa aggctttgcg gggactagcc cccgcacccc
      661 cagtctcact tgcgacgccg cgggggcagg gggaacggcg caaaaggcgc gcgccttacc
      721 acctgccctt gcggcagaat gtgttctttt ggcggggcgg caaggggtat ccaaaaagat
      781 ttataaagac gataaagccg tctttacaaa tctttctgga cgtcctcccc ctgccttggt
      841 acaagttact gaagcccggc ggtgctgcgc ctgctagact tcacgagata ctgtgcggat
      901 acaaaaaaag gcggcaaccg cccaagcaag ggcgagaagc atgtacctta gccgttcggc
      961 tatggtacat gcgttctcaa agctgaacgc gaactgcctg cttgaatcaa gcacagtcac
     1021 tgtgaaagtg acaggtgcgg gacactgtgc ggaatcttga aagattcctg atttctgaaa
     1081 ctctacattg acggtttcag acggcagatt taaatcttct gccggattgg actcgggcag
     1141 cctgtcgcaa gcgagaatgt cggggaagaa tttgcacaaa aggccgccat ctttgccgtc
     1201 ctttccgtct ttgccgtccc tgccgtttgt gcgtcccgga acggcggggg aatcgggtct
     1261 tgtgccgggc tgtccgtccg tatcgggatt tgcatcggga ttcaaatcgg ggtcgggttc
     1321 gggattgggg ctcgtgccgg ggttctcatt ggggttcggg ttgtttgcgg ggttttcggc
     1381 gggcgatact tcgggcagcg gctgtgcgtt cggtgcttcc gcgcttccgg gggtcaagtc
     1441 gggacgcggg attacttgaa catccaccgt ggtgttgcct tgcgaatccc tgccgaatgt
     1501 tgcgacaacc tgaacgggat tcccgttcct gtccgtgacg ggacccatat t
//
LOCUS       DAWHRS010000234         1544 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWHRS010000234 DAWHRS010000000
VERSION     DAWHRS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.234"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(123..551)
                     /locus_tag="WIB82_002062"
     CDS             complement(123..551)
                     /locus_tag="WIB82_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ3843305.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(532..966)
                     /locus_tag="WIB82_002063"
     CDS             complement(532..966)
                     /locus_tag="WIB82_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ3843306.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
     gene            complement(1123..>1544)
                     /locus_tag="WIB82_002064"
     CDS             complement(1123..>1544)
                     /locus_tag="WIB82_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ3843307.1"
                     /translation="HTHTHFDGKDQVAEQIKGIGSITTATLMAMLPELGRLSHKRIAG
                     LAGIAPHPREGGETKFKSRCFGGRSAVRKALYMATVAATRFEPLIRDFHQRPLSEGKP
                     YKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT          382 a          460 c          311 g          391 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtccc gaacggtaga tgtttcaccc
       61 ctgcaaacca aaaaaaagac cgtctgaata cagacggcca aagcctggtc acacatcact
      121 gcctaaaata aagctgcctg ctgtgccgca ggtcggctca tttcattgat aatcgtatat
      181 cccgttcgtg aagagatgcc gtatttaggg catagcttcg tcatcgccat aagcccgctc
      241 tccttatcaa tatcgcgcaa tttgacaaac tcctgataaa acctatggtt tctcaactgt
      301 attaacgcct tgccgcaccg tgggacatac aattcctcgc caccatatac ctgcaacagc
      361 tcatgtgttt tcacttcgcc gatggcttcg accaaaattg ccaaacgctc ggtgtccacc
      421 ttgcccttac caaatttaaa ccgcgccccg ccaatcgcct tgaccagctg ttccgtcgct
      481 gccagtccga tgacatccac aatgtccaac acggtatccg gcaataaatg ttcaactttt
      541 tcgaacccca tcatccccac ccgctttttc cgttttcctg ttttccgcaa tctgcaacgc
      601 agcaaccagt ttatgtagct gcgtatcgtc taaatattcg accttatcct taccaaacat
      661 ccgccgcgcc attgcgtgtg catagttcca atgtttgccg ccgacggtca gcagggcttc
      721 gactttgtcc aacattgccg ctgatgatgt ccgacgcaga tgtggtttac cgtgtgggtt
      781 accttttgct ttaggcttaa atccgtgcga ccgcatatca gcgacaacag actcaagttc
      841 ggaaacatcc atatccgcac acgaccgctt gcccgtcacg cgctccaaca ccgcgcgata
      901 ggtaccgtcg tccaagccca gctccttttg ggcaatttta atttttgcaa tcaacgcccg
      961 gcgcatctca aacccataaa acacaatata ttgactaatt aacgcatatt ataccaataa
     1021 aatacaatat atagtattaa gccgatgttt tttgcgaaac ggacagacat gaaaaagcaa
     1081 ctgtattttt caccccgtcg ggcaaaaata ccaaaactca aatcaagccg tccggatacc
     1141 gttttcggcg gtatcgtttt cggcaaaata atcacgcatc cgggcattcg atatcgtcag
     1201 cagtttgcgc atacatgccg taacggcaac cttatacggc ttaccctcgg acagcgggcg
     1261 ttggtggaaa tcccgaataa gcggttcaaa acgtgtcgct gccacggtag ccatatacag
     1321 tgctttacgc accgcagacc ttccgccaaa gcagcggctt ttgaatttgg tttccccgcc
     1381 ctccctcggg tgcggggcaa tgccggccaa acccgctatc cgtttgtgcg acagccgccc
     1441 caattcgggc aacatcgcca tcagcgtagc cgtcgttatc gaaccgatgc ctttgatttg
     1501 ctccgccact tggtctttgc cgtcaaaatg cgtgtgggtg tggt
//
LOCUS       DAWHRS010000235         1542 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWHRS010000235 DAWHRS010000000
VERSION     DAWHRS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.235"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            197..1542
                     /locus_tag="WIB82_002065"
                     /pseudo
     CDS             197..1542
                     /locus_tag="WIB82_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575393.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          491 a          371 c          326 g          354 t
ORIGIN      
        1 ccctgatcta agcgacacag ccggggcgaa cactgaggca atctacactt cagacggcat
       61 taccgccaac tctacccaac tcgaacagct caaaaaactg tttcccgcct gttttgacgc
      121 agacggaaat ttccttatcg acagattaca agccgaaatc gcgccgcaga ccgacatcgg
      181 acgcgaattt tacgaaatga actggctggg caaatcatat gctcgcctgc ttcgcaacct
      241 gccgcccgaa accctgataa gtgaagacaa gacgcacaac gccaagcccg aaaatgcagg
      301 cagccaaaac ctgctgattc gtggcgacaa tctagaagtg ttgaaacact taaaaaacgc
      361 ctacacaaac agcgtgaaga tgatttacat cgacccgccc taaaacaccg gatcagacgg
      421 ctttgtctat caggacgacc gcaaattcac acccgctgaa cttgcccgcc tagccaatat
      481 tgatgaagac gaagccgcgc ggattttaga tttcaccgac aaaggctcaa actcgcacag
      541 cgcatggctc acctttatgt atccgcgcct gtatgtcgcc cgcgaactgt taaaggacga
      601 cggtgtgatt tttatctcga ttgacgataa cgaagcggcg cagttgaaat tgttgtgtga
      661 tgaagtgttt ggggaaggga attttgttgc acaattgcct tggcgaaaaa gaacagctaa
      721 atcagatgtg ccttttggta tttcgcagga ttatgaatgg atatttgtat tcgcaaaatc
      781 ttgccaattt attgcagcaa ctaaaggcaa ggaacgacgc tattatgaga ctgatgattt
      841 ccccgatcgt ccttggcgta cccacgactt aacgaaacaa acaactgcgg cggaaaggcc
      901 aaatagtttt ttcacaatgg ttgatcccaa gacaggaaaa aaatatccag caaatccaaa
      961 tgcaacttgg cgtgtaacca aagatacatt tcaagattat tataataaag gaaaaatcgt
     1021 tttccctgat gattatgatt tcttaaatat cagcaatcca gttatgcgtt actttaaaga
     1081 tgacgacatg aaaaaagctg gcgaggattt tggcaaagta gctgtaagca gtagattacc
     1141 tgaaaatgtt ggtacattag ccgatgcagt agccgaatat ttggctattt ttagtaggac
     1201 gctacctgaa aatatcggaa tgactaaaga aggcacaaaa gaaatcacag atttatttgg
     1261 tagcaaaata tttaccttcc ccaagcctag tcaattgatt aaatttttag tttcaataag
     1321 ttcaaagagt aatgacctaa tcctagactt cttcgcaggc agcggcacaa ccgcccacgc
     1381 cgtgatgcag cttaacgccg aaggacaaaa cggtaaccgc cgctatatct gtgtacagct
     1441 tcccgaaaaa accgctgaaa aatccgaagc ccgtaaagca ggctacccga ccatcttcga
     1501 catcaccaaa gcccgcatag aaaaagccgc cgccaaaatc cg
//
LOCUS       DAWHRS010000236         1506 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWHRS010000236 DAWHRS010000000
VERSION     DAWHRS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.236"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            3..1433
                     /locus_tag="WIB82_002066"
     CDS             3..1433
                     /locus_tag="WIB82_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ3843308.1"
                     /translation="MHRPPFYGNRNDPFRTEKTVLLLGTAFAAASVHASGYHFGTQSV
                     NAQSTANAAAAEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGL
                     PVQGSKSGKITKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKL
                     GLTSIAVEPVAAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNG
                     VAEAAKIQADGHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAA
                     DGAAAKAMWSTMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHS
                     RFDKAELVFEKEKTVVKGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKS
                     PVRNADYRMNSLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDV
                     DSKGASSARFKNHADIIGLQYTYKFK"
BASE COUNT          394 a          486 c          360 g          266 t
ORIGIN      
        1 ttatccaccg tccgcctttt tacggaaacc gaaatgaccc cttccgcact gaaaaaaccg
       61 tcctgctgct cggcactgcc tttgccgccg catccgtcca cgcatccggc taccacttcg
      121 gcacacagtc ggtcaacgcg caaagcacgg caaatgccgc cgccgcagaa gccgccgacg
      181 catcgaccat cttctacaac cctgccggcc tgaccaaact cgacagcagc cagatttccg
      241 tcaacgccaa catcgtgctg cccagcattc attatgaggc ggattccgcc accgacttta
      301 ccgggcttcc cgtccaaggt tcgaaaagcg gcaaaatcac caaaaccacg gtcgcgcccc
      361 acatctacgg cgcatacaaa gtcaacgaca atctgaccgt gggcttgggc gtgtacgtcc
      421 ccttcggctc tgccaccgaa tacgaaaaag attccgtgtt gcgccacaac atcaacaaac
      481 tcggtctgac cagcatcgcc gtcgaacctg tcgccgcgtg gaaactcaac gaccgccatt
      541 ccttcggcgc aggcatcatc gcccaacata cttccgccga actgcgcaaa tatgccgact
      601 gggggattaa gagtaaagca gagatattga cggcaaaacc gcccaaacct aacggtgtag
      661 ccgaagctgc aaaaattcag gccgacggac acgccgatgt caaaggcagc gattggggct
      721 tcggctacca actggcgtgg atgtgggaca tcaacgaccg tgcgcgcgtg ggcgtgaact
      781 accgttccaa agtctcgcac acgctcaaag gcgatgccga atgggcggca gacggcgcgg
      841 cggcgaaagc aatgtggagt acgatgcttg cagcaaacgg ctacacggcg aatgaaaaag
      901 cccgcgttaa aatcgttacg cctgagtctt tgtccgtaca cggtatgtac aaagtgtccg
      961 ataaagccga cctgttcggc gacgtaactt ggacgcgcca cagccgcttc gataaggcgg
     1021 aactggtttt tgaaaaagaa aaaaccgtcg tcaaaggcaa atccgaccgc accaccatca
     1081 cccccaactg gcgcaacacc tacaaagtcg gcttcggcgg ttcttatcaa atcagcgaac
     1141 cgctgcaact gcgcgccggc atcgcttttg acaaatcgcc cgtccgcaac gccgactacc
     1201 gcatgaacag cctacccgac ggcaaccgca tctggttctc cgccggtatg aaataccata
     1261 tcggtaaaaa ccacgtcgtc gatgccgcct acacccacat ccacatcaac gacaccagct
     1321 accgcacggc gaaggcaagc ggcaacgatg tggacagcaa aggcgcgtct tccgcacgtt
     1381 tcaaaaacca cgccgacatc atcggtctgc aatacaccta caaattcaaa taaacggccc
     1441 gccgtttgaa tgtaacaatg ccgtctgaaa cagatttccg tttcagacgg cattgtttta
     1501 ggctcg
//
LOCUS       DAWHRS010000237         1442 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWHRS010000237 DAWHRS010000000
VERSION     DAWHRS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.237"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..64
                     /locus_tag="WIB82_002067"
     CDS             <1..64
                     /locus_tag="WIB82_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ3843309.1"
                     /translation="AYGETGASCKSCHDSFRAPE"
     gene            complement(228..1343)
                     /gene="lst"
                     /locus_tag="WIB82_002068"
     CDS             complement(228..1343)
                     /gene="lst"
                     /locus_tag="WIB82_002068"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ3843310.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAEW
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
BASE COUNT          388 a          383 c          283 g          388 t
ORIGIN      
        1 cgcctacggc gaaaccggcg caagctgcaa atcctgccac gacagtttcc gcgcacccga
       61 ataacatccg aaacagaaca atgccgtctg aagccttcag acggcatttt ctttgccatt
      121 tcaccaagcg caaagcaatc aaaaattatc ttcaattcaa aaaatacgcc atatcctgat
      181 ttttgcctta cttactccgc cattttctat tttgatttgc tatgcgatta atctttatcg
      241 tcaaatgtca aaatcgggat gccggattgg gtaaacaggg catacaccgg cttgagccaa
      301 taatcttccg gaagggaagc cggtttcaat gcgtaaacgt gcacattggg aaaatccttc
      361 atcgtcaacg ccgcaccgct gaaaaaggta taaatttcat acctcgtatg cgggtttttc
      421 ttaatttcgc gcaaaatata gtcttcgatg acatagggcg aatttaatgt ggttacgccg
      481 gaaagcccgt aggtttggcg ggggtgcggt gcgacatatt gtatgttgaa gttttttgcc
      541 gccttttccg aaatttcctt catctccttg tcaggcgaac ccaaaagtat ccgcaccgtg
      601 ccgcccgttt cgtcccccgc cttcagttcg gacgcatcga acagcggcag gtaagtcatc
      661 ttgcggcgac cgtcgtccat aatgtttttc aaacccttga atatcgtgta atgctcgtcg
      721 gaagcattac gggttttggc gatgctccaa tctccgatca tcatccgggc aaaattccgc
      781 ttgatcgtcc cgtttacaga aaactcatca cccaaatagc tgctgctttg aattaaattg
      841 attgtcccgt cgtcaaaggt tttgatttcc gcatccgggt aagtgctcaa aaaggcggca
      901 atgctgactt tttccaaact tgccaaataa atccgcttga ccttcggcag cagcattgcc
      961 ttcaccttca gctccgccat cgtcggaatg aaattaaacg atttgttcaa accgtagggc
     1021 aggtggaaaa aatacgccca ctccgcctta tcctttatct gattgaaata ataatcgtat
     1081 ttttcattcc tgttttcaga catcagcacc acataaaacc gctcccccgg atgctgcgcc
     1141 ataatccttt ctgccacctt catctgcaat atggtatagc agaaaatcag attgaccggt
     1201 tccccctctt cattgaagag tttgtccttc agcagggaaa ccgcattcct ttccccctga
     1261 tttacccggt caaatgtata aaatatcccg aaacaaaaaa caatcaggca caacacggtc
     1321 aaacaagcct ttttcaagcc catatcccta aaactccatt ccgacaaatt gaacatacgc
     1381 cccgagtgtc ccgggacgga aaaatgcgga attttagcag atattccgac agttaccgaa
     1441 ta
//
LOCUS       DAWHRS010000238         1400 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWHRS010000238 DAWHRS010000000
VERSION     DAWHRS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.238"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(41..1030)
                     /gene="porB"
                     /locus_tag="WIB82_002069"
     CDS             complement(41..1030)
                     /gene="porB"
                     /locus_tag="WIB82_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ3843311.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFMQYGGAYKRHQNVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVKDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVSTAGGVGLRHKF"
BASE COUNT          319 a          318 c          368 g          395 t
ORIGIN      
        1 gtctggcgac aggctttttg ttgataccaa tctttgcaga ttagaatttg tggcgcagac
       61 cgacaccgcc ggcagtcgat acgaatttgt tttcgccttt accttcttgc aaccaaccgg
      121 cagaaaccaa ggcagaagtg cgtttggaga agtcgtattc cgcaccgaca accacttggt
      181 cgtaatcgtt ggtgtagtct gcatcatcaa acgagccttt gaagccgtgg gcgtaagaaa
      241 cgcggggcgt tacgttgccg aagcggtatg ccaaggtagc ggcaacttcg gtttgagagt
      301 tgtgcgaatt gtctttaacc agtttcgcgt cttgttgctg tacggctacg gaagcgtaca
      361 gggcatcatt gtcgtaaccg ctgaccaaac ggtgaatctg gtatttctca atcttcacgt
      421 tatccacatt ctgatgtctt ttataggcac cgccatattg catgaagaag ccgccgtttt
      481 tgtagttgaa gccggcgtgg taagattcgc tgttatgtct gcctgcattg tcgttaagcg
      541 cgtattgtac gctgccgctg aggccggcaa attcgggaga atcgtagcgt acggaaatga
      601 ggcgtgcttc gggttcggca attttgttta cacccaaata gtcgcttttg ctatcccaag
      661 gattgatgtc gccggtgtct ttcaggacgc tgttcaaacg gccgacgcgc aatttaccga
      721 agccaccttt caaaccgatg aaggattggc ggttgcccca accggagtca gtaccggcga
      781 tagatgcctt ttgctcaacc tgccaaatgg ctttcagacc gttaccgagg tcttcttggc
      841 ctttgaagcc gattttcgaa cccaaatcaa cgatgccggt accggtttca acgctagccg
      901 cctgagctcc attgtgagct acggagcggg aagtttctac gccggctttg atggtgccgt
      961 acagggtaac gtcagccatt gctgcaacag gaagggctgc caaagtcagg gcaatcaggg
     1021 attttttcat tgctgtattc cttttttggt taagaaattt aagcaggccg ggctttccaa
     1081 gccgcttagc tttgcattta ccgccgacgt tttcggcgat tccatgtctg aaactataga
     1141 tgttcctgtc tgaaaaaaca aacttctgcc tgtttttgtg tggaattcga tgtgtatttt
     1201 gaagggcgga tagagatatt gtgggtattt tttgttttat aacatatggt tatttaaatt
     1261 ttttaagatt tgcattttta caacacttac tcgggagggt attggagggc attgcaaacc
     1321 gggggttata aagacggcaa aaaacgcaca cgcggtttct ttcggaacgg gcggatgcaa
     1381 aacccgaccc tgcggcagga
//
LOCUS       DAWHRS010000239         1397 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWHRS010000239 DAWHRS010000000
VERSION     DAWHRS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.239"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            286..1209
                     /locus_tag="WIB82_002070"
     CDS             286..1209
                     /locus_tag="WIB82_002070"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ3843312.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          340 a          349 c          331 g          377 t
ORIGIN      
        1 tttaggttgc tgtttttggt tttctgtttt ggttttatgt ccttgtggga atgacggttt
       61 acgcgaaaat gccgtccgaa aggttttcgg acggcatttt ttgcgttttt caggaggggg
      121 gcggcttcgt cgccttgtct tgatttttgt taatccacta tacattttta tgcggcttac
      181 gtattgaaaa cggatacatt ggcaaatact atggaaaaac atagcttgtt tttccggttt
      241 tatcgtatta ttccaaaact taccccattg ataaggaagc ggtttatgct gcaaagaact
      301 ttggcgaaat ccatcagcgt taccggagtc gggctgcatt cgggcgaacg cgtcgcgctg
      361 accctgcacc ccgcgcctga aaacagcggg atttccttcc gccgtaccga tttggacggc
      421 gagatgggcg aacaaatcaa gctgacccct tatttgatca acgatacccg cctttcctcc
      481 accatcgtta ccgacaaagg cgtgcgcgtc ggcacgatcg aacacattat gtccgcgctg
      541 tccgcctacg gtatcgacaa cgcgctgatt gagctgaacg cgcccgaaat cccgattatg
      601 gacggctcca gcctgccgtt tatttacctt ttgcaagatg cgggcgtggt cgatcaaaag
      661 gcgcaaaagc gttttttgaa aatcctcaag cctgtcgaaa tcaaagaagc gggcaaatgg
      721 gtgcgcttta cgccgtatga cggctttaag gtaacgctga ccatcgaatt cgaccatccg
      781 gttttcaacc gcagctcgcc cacttttgaa attgatttcg ccggcaaatc ctacatcgac
      841 gaaatcgcgc gcgcgcgcac tttcggcttt atgcacgaag tggaaatgat gcgcgcccac
      901 aatctgggtt tgggcggcaa tttgaacaac gctatcgtga ttgacgacac ggatgtcctg
      961 aatcctgaag gcttgcgcta tcccgatgag tttgtgcgcc acaaaatcct tgatgccatc
     1021 ggtgatttgt atatcgtcgg acatccgatt gtcggcgcgt ttgaaggcta caaatcggga
     1081 cacgccatca acaacgcact cttgcgcgcg gttttggcag acgaaacggc ttacgaccgg
     1141 gtggaatttg ccgacagcga tgatttgccc gacgcatttc acgagctgaa catcagaact
     1201 tgtggataac gctttctatt ttttccggta aattcctaaa acttaaaatt tcatcattcc
     1261 tacaaggaca aaaaaccaaa acagaaacct aaaattcgtc attcccgcga acctacatcc
     1321 cgtcattccc acgaaagtgg gaatctagaa tcccggactt tcagataatc tttgaatatt
     1381 gctgttattc taaggtc
//
LOCUS       DAWHRS010000240         1395 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWHRS010000240 DAWHRS010000000
VERSION     DAWHRS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.240"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            128..1258
                     /locus_tag="WIB82_002071"
     CDS             128..1258
                     /locus_tag="WIB82_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ3843313.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGRGDLERLAARDIIRFPGFVNNVSEVIKEH
                     HIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAE
                     KMIYFIENREAIRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          381 a          330 c          323 g          361 t
ORIGIN      
        1 ggcaacgccg taccggtttt tgttaatcca ctataatacg caggcaaacc caagccttgc
       61 tgggttacag gatgaaaccc tgccgtagca gaccgcattt ccaatccata ttcaaggtgt
      121 ccgccttatg aaaatcgttt ttatcacaac agtcgcatcc agcatttacg gtttccgcgc
      181 ccccgtcatt aaaaaattaa tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt
      241 ttccgataat gaattggata ttatcaggga aatgggggtt acacccgtta cctaccgttc
      301 aaaccgcagc gggctgaacc cgttttcgga tataaaatcc accttcctca tctttaaaga
      361 actcaaaaaa atatcgccgg atttggtttt cccttatttc gcaaaacccg tgattttcgg
      421 cacttttgcc gcaaaactgg caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg
      481 attcgcattt accccgcagc cggaaggcat accgttaaaa acaaaaatca taaaggggat
      541 tttgattgcc ttataccgca ttgccctgcc gatgttggaa agcctgattg tattaaaccc
      601 cgacgacaaa gacgaactga cggacaaata cggcatcaaa ataaaaaaca tccatatttt
      661 gggcggaatc ggtctggatt tgcggcaata tccttattcc gaggcggata ttcccgatga
      721 aaaagaaccc gtaaaatttc tctttatcgg cagatttctg aaagaaaaag ggattgatga
      781 ttttattcgg gcggcggaac aggttaagga caaatacccc gatacggttt ttaccgcttt
      841 gggcgcaatc gacaaatcac gcggggggag gggcgatttg gaacggcttg ccgcccgcga
      901 tattatccgt ttccccggtt ttgtgaacaa tgtttccgaa gtgataaaag aacatcatat
      961 attcgtattg ccgtcttatt atagggaagg cgttccccga agcactcagg aggcaatggc
     1021 cgtcggcagg gcggtgatta cgacggatgt ccccggatgc agggaaacgg tcgccgacaa
     1081 ggtcaacggc ttcctgatcg aaccttggaa tccccgcatc ttggccgaaa aaatgattta
     1141 ttttatcgaa aacagggaag ccatccgcct gatggggaat gcaagttatg cgattgccaa
     1201 agataaattc gatgccgaaa aagtcgattt gaaattactc gatattttga aggcgtaaac
     1261 aaggctgccc gcttttgagt ttcggtcatt tctgataaac ccccgtcatt cccgccacac
     1321 ccccgtcatt ccctcgaaag cgggaattca ggttcgttta gtttcggtca tttccgataa
     1381 attcttgcaa ctttg
//
LOCUS       DAWHRS010000241         1355 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWHRS010000241 DAWHRS010000000
VERSION     DAWHRS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.241"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(63..239)
                     /locus_tag="WIB82_002072"
     CDS             complement(63..239)
                     /locus_tag="WIB82_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843314.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(242..>1355)
                     /locus_tag="WIB82_002073"
     CDS             complement(242..>1355)
                     /locus_tag="WIB82_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ3843315.1"
                     /translation="DTLTALQMLAKAWRENVNPFVFGITGSGGKTTVKEMLAAVLRRR
                     FGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAVIEMGMNHFGELAVLTQIAKPDAAL
                     VNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDGMALIPQEDANMAVFKTATLNLNTR
                     TFGIDSGDVHAENIVLKPLSCEFDLVCGNECAAVVLPVPGRHNVHNAAAAAALSLAAG
                     LSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDDTYNANPDSMKAAVDVLARMPAPRI
                     FVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAYFVGDNSVEAAEKFGADGLWFAAKD
                     PLIQVLRHDLPERATVLVKGSRFMQMEEVVEALEDK"
BASE COUNT          310 a          408 c          326 g          311 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac gacttttgca
       61 gattaacgtg cagagacttt cccaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccacccaagt ctaaacataa atcttcataa agatacgcct gcgtctgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg acttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gggcgtacgc gccgacttcg gcgtgcatgg
      481 cggcggcttc gtcctcaccc agttcgccca tatcgcccat cacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca accgcagctt tcatgctgtc agggttcgca ttataagtat
      601 cgtcaatcag ggttgcgccc ttgattccgg atttgacgtt cagacggcct ttgatattgc
      661 tgaagccttt caaaccttcc gccacatcgt tcaaactcaa acctgcagcc aaagacagcg
      721 cggcggcggc ggcggcgttg tggacattgt ggcggccggg aacgggcaga accacggctg
      781 cgcactcgtt gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag cgccatgccg tctgaacata
      961 agccttgata aatctcgctt ttcgctttgg caatatcgcc cactccgtcg aaaccgcagc
     1021 cgacatgggc gcgcatggcg ttgttgacca atgcggcatc gggtttggcg atttgtgtca
     1081 aaaccgccag ttcgccaaaa tggttcatac ccatttcaat cacggcatag cggtgttttt
     1141 cgtttaattt caacaaagtc aacggcaatc cgatgtggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc attatcgccg aaacggcggc gcaataccgc agccaacatt tccttcaccg
     1261 tcgtcttgcc gcccgagccg gtaataccga acacaaacgg gttcacattc tcgcgccacg
     1321 ccttcgccaa catttgcaac gcggtaagcg tgtca
//
LOCUS       DAWHRS010000242         1296 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWHRS010000242 DAWHRS010000000
VERSION     DAWHRS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.242"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            307..>1296
                     /locus_tag="WIB82_002074"
     CDS             307..>1296
                     /locus_tag="WIB82_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3843316.1"
                     /translation="MNNQKLPEVKWGNDYSSLTDKSNEREVTHTSKFVVAKKHISFSF
                     NNTDEVVAEKKDAVVFGAATYLPPYGKVSGFDTAKLAERKNALDQIGTTHPGLIGYNY
                     QDVTCASGNCPELSYRTQFTFNNGLKRNGNGGRLDIYEDKSRDSSPIYKLKDYPWLGV
                     SFNLGGESSFKPKRQGSLVSSFSEDVTQQNGTQDQYKSKNLVYTTDDYNSKGNKNHQD
                     KHHAVAFYLNAKLHLLDKKQIKNIAQGVTVDLGTLKTRIEPTDAWKNRQGSYFNNGTW
                     TYEEKGSVSVKLKLPEVKAGRCITTPNPNPKSKAPSPALTAPALWFGAGQDGKA"
BASE COUNT          391 a          305 c          324 g          276 t
ORIGIN      
        1 ccgttgggca tctgcagcgg tttgctaaaa aacgctttac cgtgatcagt gtgcaaagtt
       61 aaaatgggga agtaagcttt tcaatcagca attcggcggg cgcggaatcg ggcggtttac
      121 cgaaccccgg cgttcgcggc gcgccgcccc gtcccgtgaa ggcaaactca aggaataaaa
      181 gatgaataaa acttggaaac ggcaggtttt ccgccatacc gcgctttata ccgccatatt
      241 gatgttttcc cataccggcg gggggggggg caggcgcaag cgcaaacgca taaatacgct
      301 attgtaatga acaatcaaaa gctgcctgag gtaaagtggg ggaacgacta tagctcattg
      361 acggacaaaa gcaatgaacg cgaagttacc catacgagta aatttgttgt agcaaaaaag
      421 cacattagtt tctcattcaa taataccgat gaagttgttg ctgaaaaaaa agatgctgtc
      481 gttttcggcg cggcgactta tctgccgccc tacggcaagg tttccggttt tgataccgct
      541 aagctggccg agcgcaaaaa tgcccttgat cagattggta cgacccaccc tgggttgata
      601 ggctacaact accaagatgt cacttgcgcc agcggaaact gtcctgaact tagctataga
      661 acccaattta ccttcaataa cgggttgaaa agaaatggaa acggcggcag gctggatata
      721 tacgaagaca aaagccgcga cagttcgccc atttacaaat tgaaggatta tccttggttg
      781 ggcgtgtctt tcaatttggg cggcgagagc tccttcaaac caaagagaca aggttctttg
      841 gtatcttctt ttagcgagga tgtgacgcag caaaatggta cacaagacca atacaaaagc
      901 aaaaacctcg tttatacgac agacgattac aatagtaagg gtaataaaaa ccatcaggac
      961 aaacaccacg ccgtcgcctt ttatctgaac gccaagctgc atttgctgga taaaaaacaa
     1021 atcaaaaata tcgcgcaagg cgtaacagtt gatttgggta ccttgaaaac acgcatcgag
     1081 ccgacggacg cgtggaaaaa caggcagggg agctatttta ataatggtac ttggacgtat
     1141 gaagagaaag gatcagtcag cgtcaaactc aaattgccgg aagtcaaagc aggccgctgc
     1201 atcaccacac cgaaccccaa tcccaaatcc aaagcccctt cgccggcact gaccgccccc
     1261 gcgctgtggt tcggagctgg gcaagatggt aaggcg
//
LOCUS       DAWHRS010000243         1241 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWHRS010000243 DAWHRS010000000
VERSION     DAWHRS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.243"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            198..416
                     /locus_tag="WIB82_002075"
     CDS             198..416
                     /locus_tag="WIB82_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ3843317.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            complement(559..>1241)
                     /locus_tag="WIB82_002076"
     CDS             complement(559..>1241)
                     /locus_tag="WIB82_002076"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ3843318.1"
                     /translation="NGRYLVLFDPLDGSSNIDVNISVGTIFSILEKPDGELKTESFLQ
                     AGRHQLAAGYVLYGPQTQLVFTFGHGVYMFTLNAENEFVLTKENPKVPESTKEFAINM
                     SNRRHWLPPVQQYIDELLTGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKR
                     DPAKPGKLRLMYEANPMSLILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVA
                     YLDRLHAK"
BASE COUNT          300 a          259 c          346 g          336 t
ORIGIN      
        1 atccactata cagtaggaaa ggctgaaaat ttatgcgtaa agcgtgatat tgtcaacgtt
       61 tttatcaacc ggacggcggt gttaaaagaa aattttgccg tatccgataa aacactggat
      121 aaaaatatta tctttgttat aattaatgta aagattcaat ttgacttttt aaccgtaaac
      181 caagagagga aagcgatatg ttcccagaat accgtgattt gatttccaaa ttgaaacagg
      241 aaaattcccg cttcgcccgt ctgttcgacg aacacaacga gctggacgat aaaattaccg
      301 gtctggtcaa caatccggtt accagcggtg cggaaaccat cgatgagctg aaaaaagcca
      361 aattgaaact gaaagacgag ttgtacgcca tcctgcaaaa agcagcggga aaataattcg
      421 ggtttgagtt tttgaaatgc cgtctgaaat gtgttcagac ggcatttttg tcatttgacc
      481 ggaaggcttg tgctgtttga aataacggcg gcggtatcgg attgccgccg ccgtgtactt
      541 gtgtgaacgg ctgtctgtct attttgcgtg caggcggtcg agataggcga cttcttcgct
      601 gctgcccatg aagacggcga cgcgttggtg caggttttcg ggctgtatgt cgagcatggc
      661 ttgatatgcg ttgcttgccg atgcgcccgc ctgttcgagt atcaggctca tagggttggc
      721 ttcgtacatc aggcgcagtt tgccgggctt ggcggggtcg cgtttgtctt gcggatacat
      781 gaacacgccg ccgcgcatca ggatgcggtg gatttcggca accatgctgg ctacccagcg
      841 catattgtag tttttgccgc gcgtgccggt ttcgcccgtc aagagctcgt cgatgtattg
      901 ttggacgggg ggcagccagt ggcggcggtt ggacatattg atggcaaatt ctttggtact
      961 ttcgggtact ttcgggtttt ctttggtcag cacaaattcg ttttcggcat taagcgtgaa
     1021 catatatacg ccgtgtccga atgtgaatac gagctgggtt tgaggcccgt agaggacata
     1081 gcctgcggca agctggtgcc ggccggcttg caagaaggac tcggttttta attcgccatc
     1141 gggtttttca aggatggaga aaatcgtacc gacggaaatg ttgacatcaa tattggacga
     1201 tccgtctaaa gggtcgaata ggacgagata gcgtccgttt t
//
LOCUS       DAWHRS010000244         1232 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWHRS010000244 DAWHRS010000000
VERSION     DAWHRS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.244"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            40..372
                     /locus_tag="WIB82_002077"
     CDS             40..372
                     /locus_tag="WIB82_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ3843319.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDMPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            704..1213
                     /locus_tag="WIB82_002078"
     CDS             704..1213
                     /locus_tag="WIB82_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ3843320.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          329 a          231 c          293 g          379 t
ORIGIN      
        1 ccttgttttg acggaaaacc atcatataaa ggaacactta tgcagatttt atcttttcaa
       61 ccggacattg cggaacgtat gctggaaggt acggaaggcg agtcggtcaa cgaaaacgca
      121 caattcgtcc gtacggacaa cggctattgg attgcgtggc atgaaggcgt agcggcactg
      181 cttgcgcccg atatgccgcc gggcattccc tgtttttggg tggaaggcgc ggaaagcctt
      241 gaagagttgt gcgtcatggt ggaacgcggc gagtttgacg aagtggaaga gtttgacggc
      301 gatgacgacg aatggctcga aacggcacag ggttgcgggc accacggcga cgcttgcgcc
      361 tgcggacatt aaaggcattg caggcttgcc gcaagggggg caaggctttg ccgtttttta
      421 aataatcggt ggattgcatc tgtttgcaat gggctgaacg cttccctttg tattttattg
      481 aataaaaaaa cttatcttgg tattataatt aaggcagcat caattatttt gggatggcaa
      541 taaacgcaaa gcattgattt gcgccgattg cagacttatt atagcaggtt gcggcgcgga
      601 cttaatggga attgtttata gagcatgatg gtctgaaaca tcattaatta aaaataccat
      661 taatccgatt tatatttatt tcaatttcaa tggaaaaaca tcaatgacaa tgattttaag
      721 cattttaagc ctgtttttta tcatcagact gttattttta gccgtctcta ttaaacatga
      781 aaaagccttg attgccaaag gggcaaaaca atacggaaaa accaattcca cgctgcttgc
      841 ggcagttcat acgctttatt atttggcgtg ttttgtttgg gtatggcttt ctgacactgc
      901 ttttaatggc atatccttga ttggtacgct gacggtgatg gcttcgtttg tgatattgtc
      961 attgattatt aagcagttgg gggagatttg gacggttaaa atctatattt taccaaatca
     1021 tcaaattaat cgttcgtggt tgtttaaaac attccgccac cccaattatt ttttaaacat
     1081 catacccgaa ctgattggca tcgccttatt atgtcaagcg tggtatgttt tattgattgg
     1141 cctgcccatt tatttgctgg tcttatttaa gcgtatccga caagaagaac aggcgatggc
     1201 aacacttttt taacccgttt catcaattat ag
//
LOCUS       DAWHRS010000245         1197 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWHRS010000245 DAWHRS010000000
VERSION     DAWHRS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.245"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            21..689
                     /locus_tag="WIB82_002079"
     CDS             21..689
                     /locus_tag="WIB82_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ3843321.1"
                     /translation="MQADLAYAAERITHDYPKPTGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFGGWRIAADYASYRKWNNNKYSVNIKKGRETQHNREELKTENQENGTFHAASS
                     LGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHSVETKTTIVTSKPNGGPVPSSV
                     TKPPYHQSHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLKNTRFKTHEVSLGM
                     RYHF"
BASE COUNT          329 a          354 c          266 g          248 t
ORIGIN      
        1 gcagccgcag cccgtattat gtgcaggcgg atttagccta cgccgccgaa cgcattaccc
       61 acgattatcc gaaaccaacc ggtgcaaaca acacaagcac agtaagcgat tatttcagaa
      121 acatccgtgc gcattccatc caccctcggg tgtcggtcgg ctatgatttc ggcggctgga
      181 ggatagcggc agattatgcc agttacagaa aatggaacaa caataaatat tccgtcaaca
      241 taaaaaaggg gagagaaacc cagcacaaca gggaagaact gaagacggaa aatcaggaaa
      301 acggtacatt ccacgccgct tcttctctcg gcttatccgc catttacgat ttcaaactca
      361 acgataaatt cgataaattc aaaccctata tcggtgcgcg cgtcgcctac gggcacgtta
      421 aacatcaggt tcattcggtg gaaaccaaaa ccacgattgt tacctctaaa ccaaatggag
      481 gccctgtccc aagttcggtt accaaacctc cctatcacca aagccacagt atcagcagct
      541 tgggtcttgg tgtcatcgcc ggtgtcggtt tcgacatcac acccaagctg accttggaca
      601 ccggataccg ctaccacaac tggggacgct tgaaaaacac ccgcttcaaa acccacgaag
      661 tctcattggg catgcgctac cacttctgat tccccgatac cgatgccgtc tgaaccttca
      721 gacggcattt ttgattcacc tgccgttgac aaacgcgggg cgggcgtgga aatacccgaa
      781 ccgtcattcc cgacaacacc gtaatcttga aacccgtcat tcccgacaat accgcaatct
      841 tgaaacccgt cattcccgcg caggcgggaa tccagccccc tgacgcggcg ggaatctatc
      901 ggaaatgact gaaaccccgg gattctagat tcccactttc gtgggaatga cggttcagtt
      961 gcgttccgac aacaccgtaa tcttaaaacc cgtcattccc gcgcaggcgg gaatctagac
     1021 ctgtccgcac agaaacttat cggataaaaa cagttgccca aaccccgcgt tctagattcc
     1081 cactttcgtg ggaatgacga ttcagtttcg ttccgacaac accgtaatct cgaaattcgt
     1141 cattcccgcg caggcgggaa tccagacccc cgacgcggcg ggaatctatc ggaaatg
//
LOCUS       DAWHRS010000246         1130 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWHRS010000246 DAWHRS010000000
VERSION     DAWHRS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.246"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(43..>1130)
                     /locus_tag="WIB82_002080"
     CDS             complement(43..>1130)
                     /locus_tag="WIB82_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ3843322.1"
                     /translation="NENFAEDGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMRE
                     GKYEELKALWELIHHKAVLQYKIKDEAEFADLFTAYLHENAAKFPQAGIRTAVNEAYI
                     NNGLMLSRRIDSFEDEDFIRFNTMTYREFLEKLAQTAKIRMQTLHQAFYCIRNELNIG
                     DFLNMQTIAQIKNGFNRFLLHHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADL
                     GRFEDTEHRPAAGYLFGEIFYDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGT
                     YSPDFAYIVKTKSGEILNFVIEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVF
                     KTQFDGERIAELIGQNMPAGGHSENGR"
BASE COUNT          246 a          263 c          265 g          356 t
ORIGIN      
        1 aaaatcccga agcagacggc acgcgccccg aacattcgtt ctttaacgcc cgttttcaga
       61 atgcccgcct gcgggcatat tttgcccgat cagttcggct atcctctcgc cgtcaaactg
      121 cgttttgaac accaccttga tttctttgga aatctcggca aacagctttt cggcatgttt
      181 gattttccgc tcttcgcttt ttcgcaaatc ttccgccccg tcagtccctt tggcttcaat
      241 cacaaagttc aggatctcgc cgcttttggt tttcacgata taggcaaaat cgggcgaata
      301 cgtgccgccg ccggcaacag ggattttgat ggagtttctc ggtattttgg taaatacgat
      361 tacgccttca atttggttgt tggcgacatt ttcatgttct atatccgaat cgtagaaaat
      421 ctcgccgaag agatagccgg cggcaggccg gtgctccgta tcttcaaatc tgcccaaatc
      481 tgcttttttc accgcgcgcg gtttgccgtc tttattggta aatttggtcg gatggatttt
      541 gctgccgaca agccggtaat ccagttcgaa tttatggaag gaatgatgaa gcaaaaaccg
      601 gttgaagccg tttttgattt gggcgatggt ttgcatattc aaaaaatcgc caatgttcag
      661 ttcgttgcgg atgcagtaaa acgcctgatg caaagtctgc atacggattt ttgccgtttg
      721 tgccagtttt tccagaaact ctcggtaagt cattgtgttg aaacggataa aatcttcatc
      781 ttcaaaactg tctatgcggc gggaaagcat aagcccgttg ttgatataag cttcgtttac
      841 cgccgtgcgt atgcctgcct gtgggaattt ggcagcgttt tcatgcaaat aggcggtaaa
      901 taaatcggca aattcggctt catccttgat tttatactgc aaaacggctt tatggtgaat
      961 cagctcccac aaggctttga gttcttcata tttgccttcg cgcatgatga tggtgtcttt
     1021 gccttcgtct ttggcgttgc tgactttgcc tttgtccaaa cctttgggga aggcttcggg
     1081 ataggcggct tttaatttgt catagccgtc ttcggcaaag ttttcattgt
//
LOCUS       DAWHRS010000247         1129 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWHRS010000247 DAWHRS010000000
VERSION     DAWHRS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.247"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(232..1086)
                     /locus_tag="WIB82_002081"
     CDS             complement(232..1086)
                     /locus_tag="WIB82_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ3843323.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIAAFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVELLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          314 a          339 c          293 g          183 t
ORIGIN      
        1 atcccgtcat tcccgcgcag gcggaaatcc ggacatccaa cgctgcagca atttgtcgga
       61 aataaccgaa actcaaaaaa ccggatgccc gcctgcgcgg gaatgacgac gcaaagatgg
      121 tcgttatttc ggataaatat ccgtcttttc gttccggata cccgctttcg caggaatgga
      181 gatggaaaga tttcagccta ttcggcaatc atcaaatacc gcttcccaac tttatttttg
      241 atgcgacagc cggccggcaa ccaaggtggc ggcgatgacg acaaacacgc ccaaggcgga
      301 cacgggcgac aggtgttcgc ccaaaatcaa aaccgccagc agcacgccga cgacgggttc
      361 gagcgaaatc aacagtcccg aaacattggc aggaacacgg ctcatcccct tgttccacag
      421 ccaataggcg taccagccgc accccaaacc caaatacagc agcgacaata ccatcccgac
      481 gctccagtcc acggtataac tttgcgccaa agcaagcgaa aacggcaggc acatcaacga
      541 tgcggcggca atggaaacag atgtgaatgc cggtgcgccg atgcgtgcaa tcagcctttg
      601 cgtcggacgc atagcggcac aaaagcccgc gcccgccaac aacaccagca ggcagccgaa
      661 ccagccgact tcgccgccct cttccgcacc gcccgccatc agcagctcga caccggcaaa
      721 tgccgccgcg ccgcatatcc agtggtaggc acgcgctttg tcgttgaaga aaaagtgtcc
      781 gacaaacacc atcagcagtg gctcgagtcc gacaatgacc gatgcgctgg cggcggaagt
      841 gtatttcaac ccgacaaact gaagcagcag ggtcagcaca tagttgacaa acgccgcaat
      901 cagcaacggc ttccattcct cacgcggaat cttgccgaca tgacggcggc aggcgggcag
      961 tgcaggcagc gcggcaatta gcaggcgcac gccgaccatc aatgcgggat cgatgccgcc
     1021 atagacatat ttggcggcaa taaacgagct gctccagata atcagggcaa ggatttggta
     1081 aaacatagtg agtgataaag tcagatggtc atacaaaagt atagcggat
//
LOCUS       DAWHRS010000248         1127 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWHRS010000248 DAWHRS010000000
VERSION     DAWHRS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.248"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            82..1047
                     /locus_tag="WIB82_002082"
     CDS             82..1047
                     /locus_tag="WIB82_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3843324.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDSAPQAKEQAVSAAQTESASV
                     TVKTARGDAKVPQNPERIAVYDLGMLDTLNKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGLPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT          290 a          322 c          296 g          219 t
ORIGIN      
        1 ttatcattta tcctttctaa agccgttccg gtttgtccga ccgacggctt tgccccaata
       61 tccccatttt ggagacaccc tatgttgcgt ttgaccgcat tgaccctctg caccgtcctc
      121 gccttgggcg cgtgttcgcc gcaaaattcc gactctgccc cacaagccaa agaacaggcg
      181 gtttccgccg cacaaaccga aagcgcgtcc gttaccgtca aaaccgcgcg cggcgatgca
      241 aaagtgccgc aaaaccccga acgcatcgcc gtttacgatt tgggtatgct cgacaccttg
      301 aacaaactgg gcgtgaaaac cggtttgtcc gtcgataaaa accgcctgcc gtatttagag
      361 gaatatttca aaacgacaaa acctgccgga actttgttcg agccggatta cgaaacgctc
      421 aatgcctaca agccgcagct catcatcatc ggcagccgcg ccgccaaagc atttgacaaa
      481 ttgaacgaaa tcgcgccgac catcgaaatg accgccgata ccgccaacct caaagaaagt
      541 gccaaagagc gcatcgacgc gctggcgcaa atcttcggca aacaggcgga agccgacaag
      601 ctgaaggcgg aaatcgatgc ttcgtttgaa gcggcgaaaa ctgccgcaca aggcaaaggc
      661 aaaggtttgg tgattttggt caacggcggc aagatgtccg ccttcggccc gtcttcacga
      721 ctgggcggct ggctgcacaa agacatcggt ttgcccgctg ttgacgaagc catcaaagaa
      781 ggcagccacg gtcagcctat cagctttgaa tacctgaaag agaaaaatcc cgactggctg
      841 tttgtcctcg accgaagcgc ggccatcggc gaagagggtc aggcggcgaa agacgtgttg
      901 gataatccgc tggttgccga aacaaccgct tggaaaaaag gacaggtggt ttaccttgtt
      961 cctgaaactt atctggcggc cggcggcgcg caagagctgc tgaatgcaag caaacaggtt
     1021 gcggatgcgt tcaacgccgc gaaataatga aacggcggca ttcgatgccg tctgaaacac
     1081 ggatgcaaac cgcttcccgt gtttcagacg gcattgcccg atacggg
//
LOCUS       DAWHRS010000249         1120 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWHRS010000249 DAWHRS010000000
VERSION     DAWHRS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.249"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..890
                     /locus_tag="WIB82_002083"
     CDS             <1..890
                     /locus_tag="WIB82_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3843325.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKA
                     NLPKEAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
BASE COUNT          300 a          336 c          296 g          188 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttg
      121 ccgaagccaa agccaacctg cccaaagagg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgattgtaca ggcgggcaaa cgccgcaaca
      241 ttcaagcaag cgaagcggaa atcgatgccg tcgtcgcaaa aaattccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gattttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcagaacagc cgcgtgagcg aagctgaaat cgatgccttc ctcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaagccgac agcgaaaacg ccgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctagcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg aaagcgggaa tctagaaacg aaaagtaaca
     1021 ggaatttatc gggaatggct ggagtttaaa ggactggatt cccgccgtcg cgggaatgac
     1081 gggattttgg gttgtggtaa tttatcggaa aaacaaaaaa
//
LOCUS       DAWHRS010000250         1106 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWHRS010000250 DAWHRS010000000
VERSION     DAWHRS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.250"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..713)
                     /locus_tag="WIB82_002084"
     CDS             complement(<1..713)
                     /locus_tag="WIB82_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3843326.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKSKFFESPGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKLSTRAVPYVGTALLAH
                     DVYETFKEDIQAQGYQYDPETDKFVKGYEYSNCLWYEDERRINRTYGCYGVDSSIMRL
                     MSDYSRFPEVKELMESQMYRLARPFWNWHKEELNKLSSLDWNNFVLNRCTFDWNGGDC
                     SVNKGDDFRNGANFSLIRNP"
     gene            complement(697..1017)
                     /locus_tag="WIB82_002085"
     CDS             complement(697..1017)
                     /locus_tag="WIB82_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ3843327.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
BASE COUNT          318 a          232 c          198 g          358 t
ORIGIN      
        1 ggattgcgga taagggaaaa attagcccca tttctgaaat catcaccttt attgaccgaa
       61 caatctccgc cgttccaatc aaatgtgcaa cgatttaaaa caaaattatt ccaatccaaa
      121 gaacttaatt tattcagttc ttctttatgc caattccaaa acggacgtgc cagcctatac
      181 atttggcttt ccatcaattc tttgacttcg gggaatctgc tgtaatcgga cataaggcgc
      241 ataatcgaac tgtcaacgcc gtagcagcca taggttctat taatacgtct ttcgtcttcg
      301 taccaaaggc aattactata ttcgtagcct tttacaaatt tgtcggtttc ggggtcgtat
      361 tggtagcctt gtgcctgtat gtcttctttg aaagtttcgt atacgtcatg ggctaaaagg
      421 gctgttccga catagggaac cgcccttgtg cttaatttcg cgcctaagcg ggcaagtttg
      481 ccgactcctg ccaagactcc ggcgcgggat acgctggcgg ttatttttgc gttgattcgg
      541 gcttttgcgc ccgtggggat atgttcgaca tttgccgctt ctgtgaattt agaaaattca
      601 ttattgattt ttctatatcc tggagattca aaaaactttg atttagatgg cctaaatttt
      661 attattcgat catctattct tgctggttct gcaaaactaa gagaacaaca cattaaaatc
      721 ggtgttgcta tcagaaaatt ccgagtaaat aaattcatgc gaaatttttc cattttcttc
      781 tgacttccta ataaaaatat tagactcatc agaaaaataa attttccaaa tattatgcgt
      841 taccctctta tttaaaaaat aagagaaaca ttctattacg tcgtattctt ttacatttct
      901 tacaaactct tcaaattcct tagacgcaat taatgccatc gactgcccaa aatacttgct
      961 ggacggctga tatttataaa gtgccaactg cgcctgcgtg ataaacggct tgttcattgt
     1021 tctgcctttc aaaggttgtt ttgaaagcct gattttaaaa cacgtcattt aaatatcaaa
     1081 gcgacagaca aagccaagaa gaaacc
//
LOCUS       DAWHRS010000251         1102 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWHRS010000251 DAWHRS010000000
VERSION     DAWHRS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.251"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            143..1042
                     /locus_tag="WIB82_002086"
     CDS             143..1042
                     /locus_tag="WIB82_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3843328.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEVEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          183 a          252 c          355 g          312 t
ORIGIN      
        1 cggcaggacg gttttttgcc ccaacggaaa atagccgacc tgcccgtaaa atcagccgtt
       61 tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat ggcggcattc
      121 ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg gtaacggcaa
      181 aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg gcagtctgat
      241 tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc tgatttcggt
      301 gttcgtattt tggtttttag cacggttttt cgggcaaaaa ttcccaaaaa acaggaaaac
      361 cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt tgtggcacga
      421 aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc aaatcttttt
      481 cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga aaaaggcagg
      541 cttaatatcg gcagttgccg gtgtggcgat gattgccggt gcggaattcg gctacaacgg
      601 taatgcggtt tggggattcg tcagcggttt ggtgtccggg ctgatgcttg ccctgtcgat
      661 ggtgttcgtc cgcaagactc atgaagtcga gccggtggcg cttttccctt caatgatgat
      721 tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg atggcggcgt
      781 gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg tggtgatgca
      841 gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt cgctgacggg
      901 gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg ggttgggcaa
      961 accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa tttacctcgg
     1021 ttcgctgaaa cagcagtctt aatattgatt ccatcaaggc agtattggca gttttccgtg
     1081 caaaacctgc gttaccggac tg
//
LOCUS       DAWHRS010000252         1090 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWHRS010000252 DAWHRS010000000
VERSION     DAWHRS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.252"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>1090
                     /locus_tag="WIB82_002087"
     CDS             <1..>1090
                     /locus_tag="WIB82_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-regulated protein frpC"
                     /protein_id="HEZ3843329.1"
                     /translation="YATGRKDIIRFTDGITADMLTFTREGNHLLIKAKDGSGQVTVQS
                     YFQNDGSGAYRIDEIHFDNGKVLDVATVKKLVQQSTDGSDRLYAYQSGNTLNGGLGDD
                     YLYGADGDDLLNGDAGNDSIYSGNGNDTLDGGEGNDALYGYNGNDVLNGGEGNDHLNG
                     EDGNDTLIGGAGNDYLEGGSGSDTYVFGEGFGQDTVYNYDYATGRKDIIRFTDGITAD
                     MLTFTREGNHLLIKAKDDSGQVTVQSYFQNDGSGAYRIDEIHFDNGKVLDVATVKELV
                     QQSTDGSDRLYAYQSGNTLNGGLGDDYLYGADGDDLLNGDAGNDSIYSGNGNDTLNGG
                     EGNDALYGYNGNDVLNGGEGNDHLNGEDG"
BASE COUNT          302 a          246 c          298 g          244 t
ORIGIN      
        1 actacgctac cggacgcaaa gacatcatcc gctttaccga cggtattaca gccgatatgc
       61 tgacttttac ccgagagggc aaccatcttc ttatcaaggc aaaagacggc agtggacaag
      121 tgactgttca gtcctatttc cagaacgatg gctcaggtgc ttaccgtatc gacgagattc
      181 atttcgataa cggcaaagta ctggatgttg ccactgtcaa aaaactggta cagcaatcca
      241 ccgacggttc ggacagattg tatgcctacc aatccggaaa taccttaaat ggcggattgg
      301 gcgatgacta tctgtacggt gccgacgggg atgacctgct gaatggtgat gcaggcaacg
      361 acagtatcta cagtggcaat ggcaatgata cgctcgatgg aggagaaggc aacgacgccc
      421 tgtacggcta taatggtaac gatgtactga atggtggcga aggcaatgat catttgaacg
      481 gcgaagacgg taacgacact ctgatcggcg gtgccggtaa tgattacttg gagggcggca
      541 gcggttcgga tacttatgtc ttcggcgaag gcttcggtca ggatacggtc tataattacg
      601 actacgctac cggacgcaaa gacatcatcc gctttaccga cggtattaca gccgatatgc
      661 tgacttttac ccgagagggc aaccatcttc ttatcaaggc aaaagacgac agtggacaag
      721 tgactgttca gtcctatttc cagaacgatg gctcaggtgc ttaccgtatc gatgagattc
      781 atttcgataa cggcaaagta ctggatgttg ccactgtcaa agaactggta cagcaatcca
      841 ccgacggttc ggacagattg tatgcctacc aatccggaaa taccttaaat ggcggattgg
      901 gcgatgacta tctgtacggt gccgacgggg atgacctgct gaatggtgat gcaggcaacg
      961 acagtatcta cagtggcaat ggcaatgata cgctcaatgg aggagaaggc aacgacgccc
     1021 tgtacggcta taatggtaac gatgtactga atggtggcga aggcaatgat catttgaacg
     1081 gcgaagacgg
//
LOCUS       DAWHRS010000253         1058 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWHRS010000253 DAWHRS010000000
VERSION     DAWHRS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.253"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(15..206)
                     /locus_tag="WIB82_002088"
     CDS             complement(15..206)
                     /locus_tag="WIB82_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843330.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            335..895
                     /locus_tag="WIB82_002089"
     CDS             335..895
                     /locus_tag="WIB82_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ3843331.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          299 a          293 c          216 g          250 t
ORIGIN      
        1 cgtaccggtt tttgttaatc cgctatacca cgatgaatcc ttcgcaatat ctgtttatcg
       61 acctcaattt tgacaaaata ccggatacgc gcctttgttg cttttccatc ttccaaccaa
      121 ctgtaaatct caaacagccg gtacacgcca tgcttcagtt tcttttcctg tcggcggatt
      181 gtttcgacaa agaattgaaa atccatttca tgcaccttaa aatttaatct gcattcaaac
      241 cttttcactt tggaagcacc atttatcgga tgtcccttcg caataaacaa attttcccga
      301 taccgccgcc catttcaacc caaacccaaa acctatgaaa aacctcatcg ccttcaacaa
      361 accctatggc gttatctgcc aattttcacc gcacgaaaaa cacaaaagcc tcaaagactt
      421 tatcaatctt cccggcttct accccgccgg acggctcgac accgacagcg aggggctgct
      481 gctgctgacc gacgacggca ggcttcaggc acaaattacc gaccccaaat tcaaacaccc
      541 caaaacctac tgggcgcaac tggagggcgt acccgacgaa agccgattgg aaagcctaag
      601 aaaagggata gacttaggcg gtttcgttac ccgtccggca agcatccgca tcttgaaaca
      661 cggagaagca gattcgttat gggagcgcat cccgccgata cgcgtccgca aaaccgttcc
      721 cgatttttgg attgaaatta ccatttctga gggcaaaaac cgccaagtca ggcgaatgac
      781 cgccaaggcg ggctatccct gcctgcgtct gatcagagtg gcaagcggca ggctgaaact
      841 gtttgatttg gatttaaaac ccggggaatg ggcatacgcc ccgtttaaac cataatcacg
      901 tttatctcat catttccaca aaagtgggaa tccggaattt tatagtggat taacaaaaat
      961 caggacaagg cggcgaagcc gcagacagta caaatagtac ggaaccgatt cactcggtgc
     1021 ttcagcacct tagagaatcg ttctctttga gccaaggc
//
LOCUS       DAWHRS010000254         1034 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWHRS010000254 DAWHRS010000000
VERSION     DAWHRS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.254"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..456
                     /gene="mafB"
                     /locus_tag="WIB82_002090"
     CDS             <1..456
                     /gene="mafB"
                     /locus_tag="WIB82_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ3843332.1"
                     /translation="VELNPTKWDWVKNTGYETPAVRHMQTMDGEMAGGNRPPKSITSN
                     GKADASTQPSLQAQLIGEQISSGHAYNKHVIRQQEFTDLNINSPADFARHIENIVANP
                     SESKKLSNGRSAYWDDKSGTVVIRNPKDPDGGTAFRPKIGKQYFDDKLK"
     gene            469..678
                     /locus_tag="WIB82_002091"
     CDS             469..678
                     /locus_tag="WIB82_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843333.1"
                     /translation="MKLIGFEENLVKISLNKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLSLDKELKKIYDTWDKC"
     gene            787..1020
                     /locus_tag="WIB82_002092"
                     /pseudo
     CDS             787..1020
                     /locus_tag="WIB82_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          371 a          155 c          218 g          290 t
ORIGIN      
        1 gtagaactta acccgaccaa atgggattgg gttaaaaata ccggctatga aacacctgct
       61 gttcgccata tgcagactat ggatggggag atggcaggtg ggaatagacc gcctaaatct
      121 ataacgtcca acggcaaagc agatgcttcc acacaaccgt ctttacaagc gcaactaatt
      181 ggagaacaaa ttagtagtgg gcatgcttat aacaagcacg tcataagaca acaagaattt
      241 acggatttaa atatcaattc accagcagat tttgctcggc atattgaaaa tattgttgcc
      301 aatccttccg aaagcaaaaa attatccaat gggcgtagtg catattggga cgataagtct
      361 ggaacggttg taattagaaa tcccaaagac ccagatggtg ggacagcatt tagaccaaaa
      421 ataggtaaac agtattttga tgacaaatta aagtaaaagg aattattcat gaaattaatt
      481 ggttttgaag agaatttagt taaaattagt ctcaataaag atgagttgta tattattcaa
      541 gcaattgtag gagaaattta ttcaggcgta tgcgtggatt gtagagattt tgagataatt
      601 catggtgttg aaaaaaataa agttctttct ttggataaag aacttaaaaa aatatatgat
      661 acttgggata aatgttagct aggtagggtg ggcactcgtt gcccaccttt tgccgtatat
      721 taaaaatcct tttcaggctg gaactgagtg ggcatcaatg ccaaccctaa ttgtgctgat
      781 gaagcagtat taagacaagc atcgaatggt gattatcatg gttttccgca atcagtagat
      841 gctttttctg aaaatggaac agtaatacaa atagttggcg gagataatat tgtaaggcat
      901 aaactttata tccctggatc ttataaagga aaagatggaa attttgaata tatcagagaa
      961 gcagatggaa aaattaatca tagattattt gtccctaacc aacaacttcc tgaaaaatag
     1021 tagatagaga ttaa
//
LOCUS       DAWHRS010000255         1017 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWHRS010000255 DAWHRS010000000
VERSION     DAWHRS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.255"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>1017
                     /gene="tuf"
                     /locus_tag="WIB82_002093"
     CDS             <1..>1017
                     /gene="tuf"
                     /locus_tag="WIB82_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ3843334.1"
                     /translation="IDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMI
                     TGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVE
                     MEIRDLLSSYDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDK
                     PFLLPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLD
                     EGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFA
                     NYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRT
                     VGAGV"
BASE COUNT          263 a          281 c          269 g          204 t
ORIGIN      
        1 atcgacaacg ctcctgaaga aaaagctcgt ggtattacca ttaatacctc acacgtagaa
       61 tacgaaactg aaacccgtca ctacgcacac gtagactgcc cggggcacgc cgactacgtt
      121 aaaaacatga ttaccggcgc cgcacaaatg gacggtgcaa tcctggtatg ttccgcagcc
      181 gacggcccta tgccgcaaac ccgcgaacac atcctgctgg cccgccaagt aggcgtacct
      241 tacatcatcg tgttcatgaa caaatgcgac atggtcgacg atgccgagct gttggaactg
      301 gttgaaatgg aaatccgcga cctgctgtcc agctacgact tccccggcga tgactgcccg
      361 attgtacaag gttccgcact gaaagccttg gaaggcgatg ccgcttacga agaaaaaatc
      421 ttcgaactgg ctgccgcatt ggacagctac atcccgactc ccgagcgagc cgtggacaaa
      481 ccgttcctgc tgcctatcga agacgtgttc tccatttccg gccgcggtac agtagtaacc
      541 ggccgtgtag agcgcggtat catccacgtt ggtgacgaga ttgaaatcgt cggtctgaaa
      601 gaaacccaaa aaaccacttg taccggtgtt gaaatgttcc gcaaactgct ggacgaaggt
      661 caggcgggcg acaacgtagg cgtattgctg cgcggtacca aacgtgaaga cgtggaacgc
      721 ggtcaggtat tggctaaacc gggtactatc actcctcaca ccaaattcaa agcagaagta
      781 tacgtactga gcaaagaaga gggtggtcgt cacactccgt tcttcgccaa ctaccgtccg
      841 caattctact tccgtaccac cgacgtaacc ggcgcggtta ctttggaaga aggtgtggaa
      901 atggtaatgc cgggtgaaaa cgtaaccatc accgtagaac tgattgcgcc tatcgctatg
      961 gaagaaggcc tgcgctttgc gattcgcgaa ggcggccgta ccgtgggtgc cggcgtg
//
LOCUS       DAWHRS010000256         1012 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWHRS010000256 DAWHRS010000000
VERSION     DAWHRS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.256"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(137..769)
                     /gene="mtrR"
                     /locus_tag="WIB82_002094"
     CDS             complement(137..769)
                     /gene="mtrR"
                     /locus_tag="WIB82_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ3843335.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          244 a          218 c          260 g          290 t
ORIGIN      
        1 agattgagca taaaatttta gtaacctatg ttattgcaaa ggtctcaata tgtattttta
       61 gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt cagacggcat
      121 tgttaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag ttgtccatca
      181 ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag gaagagaacc
      241 accgccaaat cagcccgtcc aaggttgatt tgatgaagat gaccgctgtt tccttgtcca
      301 aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta attttctcgc
      361 gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc tccgtgtgtt
      421 cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt tgcagccgct
      481 cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg gcatcttcgg
      541 catcttgcgc gatgcagttt tcgatgtcgt cgcagatacg ttggaacagc gcgtcgaaca
      601 agtcttcctt atttttgaaa tgccaataga gcgcgccgcg cgttacgccg gcggcttggg
      661 cgatttcgtt gagcgaggtg cgcgcaatcc ctttgcggta aaaggtttcc aaggcggcaa
      721 gcatcaggtg ttctttggtt ttcaaggctt cggttttggt ttttctcata atggcgtttt
      781 cgtttcgggt cggtttgatg agggcggatt ataaaaaaga ctttgtaacc atgcaatcgt
      841 gtatgtataa tgaaacccat gaaattgaga ctacatctca actttgaaaa caggcataac
      901 ctgctttgca acccgttcga acattcgggt atcaaagcag gcggttttta tatcctgaaa
      961 tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta ca
//
LOCUS       DAWHRS010000257          994 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWHRS010000257 DAWHRS010000000
VERSION     DAWHRS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.257"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(8..844)
                     /gene="cysT"
                     /locus_tag="WIB82_002095"
     CDS             complement(8..844)
                     /gene="cysT"
                     /locus_tag="WIB82_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ3843336.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT          277 a          313 c          244 g          160 t
ORIGIN      
        1 gacgacctca acccttcgcg cccgaacgcc tgcccaacgc ccactgcatc acgttcagcg
       61 caaacagaat cacaaacgaa accagcagca taaacaacgc caccgccgac gcgccctgta
      121 cgtcgaactg ttccagcttg cccgtaataa tcagcggcag gatttcagaa accatcggaa
      181 tattgcccgc gataaaaatc accgagccgt attcccccgt tgcccgcgcg aacatcattc
      241 ccgcgccggt caagagtgcc ggcgtgattt ccggcagcag cacgcggcgg aacgtcgtaa
      301 agcggtttgc gcccaaagtc gccgccgctt cctcatattc gcccgataat tcctccaaca
      361 ccggctgcac ggcgcggacg ataaagggca ggctgacgac gaccagcgca atccaaatgc
      421 cgacgggcgt aaacgcgatt ttgatgccca aaggctcgaa aaaacggcct atccaaccgt
      481 taggcgcata cagggttgcc aacgcgatac ccgtaaccgc cgtcggcagc gcaaacggca
      541 aatcaaccag cgcgttcgcc aagcccttgc ccggaaattc atagcgcacc aatacccacg
      601 ccaccagcgt gccgaacacg acattggtca gcatcgcata aaacgacatc cgcaaactca
      661 gccacaccgc cgccaacacg ttcggctcgg caatcgtgtt ccaaaagccg ccccagccga
      721 tttccgccgc cttcgccgcc atcatcgcaa acggcaagac cacaagcagc gacaggcaca
      781 atacggtcag accaaggctg agtttgaagc cgggcagtac gccgggcgtt ttgagcggta
      841 acataaaaca atgctgaaaa taaggaaaag gaaggactac tttaacgatg ccgtccgaaa
      901 aacggaaaga atggaaagtt tggtgcaaag acgaatttgt tataaagcgg ttggcagttt
      961 ctcaagcggg cgcgatgttt taaaatatag tgga
//
LOCUS       DAWHRS010000258          982 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWHRS010000258 DAWHRS010000000
VERSION     DAWHRS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.258"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(464..>982)
                     /locus_tag="WIB82_002096"
     CDS             complement(464..>982)
                     /locus_tag="WIB82_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3843337.1"
                     /translation="PFTDGNGRTGRILNSLLLIEKGLLDLPILYLSRYIIENRADYYR
                     LLLGVTERQDWESWIIYILDGVADTADWTVSKIDAIRRLFEQTRQHIRTHAQGIYTHE
                     LVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELSDIGVLQEIAIGRDKLFIHPRLME
                     LLRGEGNSFTSF"
BASE COUNT          240 a          215 c          228 g          299 t
ORIGIN      
        1 taggtttcca tgcggacgcg ttcggattca cgactgcgcg gaaatgacgg gattttggtg
       61 tattccctaa aaaaataaaa aaacatttgc aactttgtta aaaataaagg ctgtgtttta
      121 acgatgtgtt gatatttaat tttagaaagg tagctattta atagttacct tttcttattt
      181 aaaaatagct ttctcaaatt ccatgaacgc ctcaatacga tatgcagatg ctctatcgaa
      241 attaagtttc aacattttgt ttattaaaca ttttatttta gccatttttc aatatacccc
      301 caaatatacc cccaatttgc acaagtcaaa attttaggca ggggttttgt tgctcccatg
      361 atacgcgggc ggatgccggc ttttcgcggg ggaaatacaa ggggtctcgg ttcgggtgtc
      421 aaaatccctg tttcgtgtta gtcatgtggg ggggaagagg gggttagaat gaagtaaagc
      481 tgttgccctc tccccgcaat agttccatta ggcgcggatg aatgaatagt ttgtccctgc
      541 cgatggcgat ttcttgcagc acacctatgt ctgaaagctc tttcaggtac ttagaggccg
      601 tctgccgttt ggctatccct gccgcttcta ggttggcaat gcgtgtatat ggctgctcaa
      661 acagaagatt taccagttcg tgcgtgtaga ttccttgtgc gtgtgtccgt atgtgttgcc
      721 gtgtctgctc gaacaggcgg cgtatcgcat ctattttcga taccgtccaa tcggcggtgt
      781 cagctacgcc gtctaagatg tagattatcc agctttccca gtcctgccgt tcggttacgc
      841 ctaaaagcag gcggtaatag tccgccctgt tttcgatgat gtagcggctc aaatacaaaa
      901 taggcaaatc caaaagccct ttttcaatca atagcaggct gttcaatatg cgccccgtcc
      961 gcccgttgcc gtccgtaaac gg
//
LOCUS       DAWHRS010000259          978 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWHRS010000259 DAWHRS010000000
VERSION     DAWHRS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.259"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            9..>978
                     /locus_tag="WIB82_002097"
     CDS             9..>978
                     /locus_tag="WIB82_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ3843338.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSAGVVGTPDKLVGESETVLKKF
                     VYECPMLNEILDKAVWETDFPFHSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSARLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFRTYSGLN"
BASE COUNT          230 a          290 c          252 g          206 t
ORIGIN      
        1 aagtctgcgt gttggaaaaa cagcactttc cgcgcttcgt catcggcgaa agcctgctgc
       61 cgcactgtat ggaaatgctg gaagaagccg gatttgccga tgccgtgcgc gccgagcccg
      121 gctttcagtt gaaaaacggc gcggcgtttt cgtggggcag tcgttatacc gaattcgatt
      181 tcaccgataa attttcagac ggccccggca cgacttatca agtacgccgc gccgtgttcg
      241 ataaaatcct gattgaagaa gccgccaaac aaggcgttga agtacgtttc gggcacggcg
      301 taaccgcgtt cgacaacagc ggcgattttg tccgcctgaa catcgaaacc gacaccggca
      361 agcgctatga actgaccgcg aaattcgtct tggacgcaag cggttacgga cgcgtgctgc
      421 cgcgcctgct aaacttggaa acgccctcgc acctgccgcc gcgccaagcg catttcacgc
      481 acatcgacga caacatcacc aacccgaaat tcgaccgcaa caaaatcctg attaccacct
      541 atccgcaaca ccgcgacgtg tggatttggc tgattccctt cggcgacaac cgctgttccg
      601 ccggcgtggt cggcacgccc gacaaacttg tcggcgaatc ggaaacagtg ttgaaaaaat
      661 ttgtttacga atgcccgatg ctgaacgaaa ttttggacaa agccgtttgg gaaaccgatt
      721 ttccgttcca ctccatccaa ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt
      781 tcgcgctatt gggcaatgcc gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca
      841 tcgcgctgca ctccgccaga cttgccgccg atctgttggc aaaacaactc aaaggcgaag
      901 ccgccgattg gcaaaccgaa tttgccgaac ccttgatgat cggcgtggac gcgttccgca
      961 cctatagtgg attaaatt
//
LOCUS       DAWHRS010000260          972 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWHRS010000260 DAWHRS010000000
VERSION     DAWHRS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.260"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            404..>972
                     /locus_tag="WIB82_002098"
     CDS             404..>972
                     /locus_tag="WIB82_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3843339.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAA"
BASE COUNT          301 a          234 c          205 g          232 t
ORIGIN      
        1 aggaatgacg gtttaaaagt tacccgaaac ctcaaaaaaa aaggctgtgt tttaatatag
       61 tgttgataga cgtacttggc ttccatgtat tcaatcgtgg aaatctatat cttcgtcctc
      121 gccgaaatag tctatgcccg atatacaatt ttgatacaca aacttggaaa tatgggtatc
      181 gtcgccggag cgatagaatg cggacagtta tatatatatg gtttttttag ggaagcgggc
      241 agatgaaggg cggattttga attaacccca tgcaaattga cttttgcggg cggttttacc
      301 tcccatatat ttacaaaagc caaattttta aacatatatc cttgatatat acacggcgta
      361 aatatatact ggaaacatct ttaaattttt tgaaatttta aatatgagca actggaaacc
      421 cgatattccc tataacgatt taccacccct gccgccaaaa caggatattg aaagcaaaac
      481 catcctgaaa cgttgtatag ccgcccgtgc atcccttgcc cgtttaaagc aggcggcaga
      541 attgataccg aatcaagcca tgctgattaa cacccttcct gttatggaag cccgtgcaag
      601 ttcggaaatt gaaaacatcg taaccaccac ggacaagctg tttcaatccc tgcaaatgga
      661 tacggaacgg caagaccctg ccacgaaaga agccctgcaa taccgcaccg ccctgtttgc
      721 aggctatgaa tcactgacga gccgcccttt atgcacacaa accgccatca tggtctgcaa
      781 cgccatcaag cacccctacg aaatggccat ccgcaaaaca ggcggcacag ccctaaaagg
      841 aggcaacagc ggaaatgttg tctatacccc gcccgaagga gaagaaacca tacgcggcaa
      901 gctggcaaat tgggagcggt ttattcacga aagcggcgat ttagacccgc ttatcatcat
      961 ggcggcggca ca
//
LOCUS       DAWHRS010000261          964 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWHRS010000261 DAWHRS010000000
VERSION     DAWHRS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.261"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            13..250
                     /locus_tag="WIB82_002099"
                     /pseudo
     CDS             13..250
                     /locus_tag="WIB82_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            581..>964
                     /locus_tag="WIB82_002100"
     CDS             581..>964
                     /locus_tag="WIB82_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ3843340.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQISEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTS"
BASE COUNT          300 a          159 c          221 g          284 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tatgctgatg ccggagacgt atattgcgtc
       61 tataacatca gactgaagca gtacactgcc tgccaggtta cccgagttga agaacacggt
      121 ggcaaaaaaa acacatgcga ccctgctggc tttgggctgg cagggcaaca aaccgcttgg
      181 ggcggaggag ctggcggatt tgaaaccgct ttacaaagac ttaaagaata atattggaaa
      241 tattgtatga acaaaaaatt aaactatatt tttatgttgg actgtttagg gttggtgata
      301 ttgtttactt gtataatagc tacttttgaa agagattatg gatttaaaat ttttactaat
      361 tctaagaggc ctgaatttta ttattggatt ggaatgtttt attatggaat tatttcttgc
      421 tggtttgatt atcaattaat ttcaacaaag gcgaattcgt ataaaagaaa agttaaacaa
      481 tataaaattt tttcagtaat attttcagtt ttgatattta tttctactat agtaaaactt
      541 taaattttgg agcaaaaatt tatgagcgat tcaattgaat atgtattggg aacgcggtct
      601 gcacatgtat aaggcaagtg ccgtcgtgcc gacgggatat gtacgggttg ggaataccgc
      661 gccgctggtc ggcgaagaca cgcaacggta tgcctctttt tggggcgacg gctacgacgt
      721 gtaccgtcag ttgagatggc agcagatatc cgaaaaacag agaaaggcat tcaaaaaagc
      781 cgccaaaagc aaaaagaccg tgatgtttgc cggacgggaa tacggcatat ccaaacaaaa
      841 tttgagcgat gtttgggacg attttgaaga cgcgatggaa ctgaaggcgt ttccctgcct
      901 gtcttcactg tttctgacca aatggcataa aaatctatat gatagtggat taacaaaaac
      961 cagt
//
LOCUS       DAWHRS010000262          910 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWHRS010000262 DAWHRS010000000
VERSION     DAWHRS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.262"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..820
                     /locus_tag="WIB82_002101"
     CDS             <1..820
                     /locus_tag="WIB82_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ3843341.1"
                     /translation="QQLNQRLQQSLRVLQMSGIELEREVENWLSDNPLLERKDPDEFS
                     DAGFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSDQEAACVHILID
                     FLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAAADLNESLILQI
                     ERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLEAALDLIASLNP
                     FPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAAHKRKTAE"
BASE COUNT          238 a          289 c          216 g          167 t
ORIGIN      
        1 ccagcagctc aaccagcggc tgcaacaatc tttgcgcgta ttgcagatgt cgggtatcga
       61 acttgaacgc gaggtcgaaa actggctgtc ggacaacccc ctgctcgaac gcaaagaccc
      121 agatgaattt tccgatgccg ggttttccca ttacactgcg cccgcgcgcc aaatcggcgg
      181 agacgaaggc gaagatatgc tgtccaacat cgccggcgag caggatttca agcaatacct
      241 gcacgcgcaa gtatgcgaac acccgctttc cgaccaagaa gccgcctgtg tccacatcct
      301 tatcgatttc cttgacgagc agggttatct gaccgacagc atcgaagaca tcctcgacca
      361 tacgccctta gagtggatgt tggatgaagc aatgctgcaa cacgcgctga ccgcattgaa
      421 aaaattcgac ccggcaggcg tggccgccgc cgatttgaac gaatcgctga tactgcagat
      481 agaaagattg ggcgaatgtg ctgccaaacc cgccgccctg catatcgtcc gaaacgccct
      541 cgacagcatt gacggcaacc gcagccaaac cctcgcacga ataaaaaaac acctgcccca
      601 aaccgacagc ggcacactcg aagccgcact cgacctcatt gcttcgctca atccctttcc
      661 cgccgccggt tttgcctcgt ccacgcccac gccgtattct gacgaggcgc tcgccaacct
      721 gctggctttc cgcggcatgg aggtttctcg ccgcaccatt gccaaataca gagaatcctt
      781 tgagattccg gcagcacaca aacgcaaaac cgcagaataa ttgccgaata atcttataaa
      841 gacaacaaac caaaagccgg catttctgcg aaagcgggaa tgccgaatcc gtccgcgcgg
      901 aaacctgcat
//
LOCUS       DAWHRS010000263          849 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWHRS010000263 DAWHRS010000000
VERSION     DAWHRS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.263"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..245)
                     /locus_tag="WIB82_002102"
     CDS             complement(<1..245)
                     /locus_tag="WIB82_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3843342.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTAHSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEPDESDFGGRRKGRR"
BASE COUNT          218 a          223 c          164 g          244 t
ORIGIN      
        1 cgtctacctt tacgccgtcc gccgaaatcg ctttcgtccg gctcgacagg gccctcaaaa
       61 acctcatcgg cagccaaggc caaatgatgg ttgataaccg tgcggatttt acggtagaac
      121 agtgctgccg aattgggatg gatacccaaa atatcggcgg cagaatgggc ggtaacttcg
      181 agtacaaaaa aacggagcag ttctttctgt acttttttct ttaatttgca gtgcgttatc
      241 ttcatatttc gagggtaaca tatctgctaa tctagtacag cccctagttt aaagctttgt
      301 ttcttaagtc cgcagagtat gccatggtta gaccttcaaa gttgagtatt gtactatttt
      361 gtttttaatt aactataaaa cgcttgtgtg aataggaaga ctgcagaata ttgctgcctt
      421 ttcctgtttg ctttaaataa acagaaaatg ccgtctgaaa ccgattttca gacggcattt
      481 gtcccttaaa acacgttttt tcaagcgcga ccataccaaa cacccacagg ttgccttcca
      541 cccttcgtac aacctttgct ccatcaaaca tccacttcct aaatactgat gtctgcttag
      601 gttgaatcgg cattcgtacg attacggcac accgcctaat ctgtacctgc gtctgttgcg
      661 cccaatacca aaggctacct gaaaatctcc caaacttttc aggtagcctt atcacgtttt
      721 cgactgtcac cacacgccca aagaccgcct gaaacccttt ttcagacgtt ccgctccgat
      781 acccccaaca aaacgccgcc cgaacgcatg ttcagacggc attttctttg cgtcgattca
      841 ttgcggtgt
//
LOCUS       DAWHRS010000264          828 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWHRS010000264 DAWHRS010000000
VERSION     DAWHRS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.264"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            50..334
                     /locus_tag="WIB82_002103"
     CDS             50..334
                     /locus_tag="WIB82_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ3843343.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     SSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            335..>828
                     /locus_tag="WIB82_002104"
     CDS             335..>828
                     /locus_tag="WIB82_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ3843344.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHHGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCA"
BASE COUNT          257 a          169 c          197 g          205 t
ORIGIN      
        1 aaacattttt ttaatgaggc aaccgtgcct tttaagaaag ggatagcaaa tgaaattgtt
       61 ggccgcattg attccgctct tgatgagcgt cgcaggccgt atattgactg cattaggctt
      121 gatggctgtt acttattcgg gtgtagatag attggtagcc cattttcagc aggcgataac
      181 cagtagcata acgggcgcgc ctcaagcgat gttgcagctc ttttatataa gcggtggcgg
      241 caccgttctt aatatcctgt ttggcgcgat cgcctttatt ctgtcattca aacaaatgac
      301 aaaactagca acctcaatcg ggaagaaaaa ataaatggca gagatctgtt tgataaccgg
      361 cacgcccggt tcagggaaaa cattaaaaat ggtttccatg atggcaaacg atgaaatgtt
      421 taagccggat gaaaacggca tacgccgtaa agtatttacg aacatcaaag gcttgaagat
      481 accgcacacc tacatagaaa cggacgcgaa aaagctgccg aaatcgacag atgagcagct
      541 ttcggcgcat gatatgtacg aatggataaa gaagcccgaa aatatcgggt ctattgtcat
      601 tgtagatgaa gctcaagacg tatggccggc acgctcggca ggttcaaaaa tccctgaaaa
      661 tgtccaatgg ctgaatacgc acagacatca tggcattgat atatttgtat tgacacaagg
      721 tcctaaactc ttagatcaga acttgcgaac attggttaaa agacattacc acattgcggc
      781 caacaaaatg ggtttgcgta ccctgcttga atggaaagta tgcgcgga
//
LOCUS       DAWHRS010000265          823 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWHRS010000265 DAWHRS010000000
VERSION     DAWHRS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.265"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(232..>823)
                     /locus_tag="WIB82_002105"
     CDS             complement(232..>823)
                     /locus_tag="WIB82_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3843345.1"
                     /translation="QGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNP
                     NENPGTSPNPEPDPDLNPDANPDTDGQPGTSPDSPAVPDRPNGRDGKDGGLLCKFFPD
                     ILACDRLPEPNLAEDLNLPSETVNVEFKKSGIFQDSAQCPAPVTFTITVLDSSKQFAF
                     SFENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
BASE COUNT          163 a          210 c          258 g          192 t
ORIGIN      
        1 ggactagccc ccacaccccc agtctcactt gcgacgctgc gggggcaggg ggacggcgca
       61 aaaagcgcgc gccttaccac ctgcccttgc agcagagtgt gttcttttgg cggggcggca
      121 aggggtatcc aaaaagattt ataaagacga taaagccgtc tttacaaatc tttctggacg
      181 tcctccccct gccttggcac aagttactga agcccggcgg tgctgcgcct gctagacttc
      241 acgggatact gtgcggatac aaaaaaaggc ggcaaccgcc caagcaaggg cgagaagcat
      301 gtaccttagc cgttcggcta tggtacatgc gttctcaaag ctgaacgcga actgcttgct
      361 tgaatcaagc acggttatag tgaacgtaac gggggcggga cactgtgcgg aatcttgaaa
      421 gattccggat ttcttaaact ctacattgac ggtttcagac ggcagattta aatcttctgc
      481 cagattgggc tcgggcagcc tgtcgcaagc tagaatgtcg gggaagaatt tgcacaaaag
      541 cccgccatct ttgccgtccc tgccgtttgg gcggtccgga acggccgggg aatcggggct
      601 tgttccgggc tgtccgtccg tatcgggatt tgcatcggga ttcaaatcgg ggtcgggttc
      661 gggattgggg ctcgtgccgg ggttctcatt ggggttcggg ttgtttgcgg ggttttcggc
      721 gggcgatact tcgggcagcg gctgtgcgtt cggtgcttcc gcacttccag gggttaaatc
      781 gggacgcggg attacttgaa catccactgt ggtgttgcct tgc
//
LOCUS       DAWHRS010000266          821 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWHRS010000266 DAWHRS010000000
VERSION     DAWHRS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.266"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..501)
                     /locus_tag="WIB82_002106"
     CDS             complement(<1..501)
                     /locus_tag="WIB82_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3843346.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKLSTRAVPYVGTALLAHDVYETFKEDIQARGYQYDTETDKFV
                     KGYEYSN"
BASE COUNT          284 a          175 c          117 g          245 t
ORIGIN      
        1 attactatat tcgtagcctt ttacaaattt gtcggtttcg gtgtcgtatt ggtagcctcg
       61 tgcctgtatg tcttctttga aagtttcgta tacgtcatgg gctaaaaggg ctgttccgac
      121 atagggaacc gcccttgtgc ttaatttcgc gcctaagcgg gcaagtttgc cgacccccgc
      181 caatacgcca gcgcgggaaa ctgatgaaga tacaaccgaa ctaactttac ccgtacttga
      241 tgctccagta gatacatgcc taacttttaa atcttgagct tctgaagtta aatatcttcc
      301 cgattctcta tcaaatacac ctttccaaat tcttcttctt ttgtcatctg aacctcgaat
      361 acctaaatat ctgccatcat cataaacttc aaccctatca aatttacccg gagcatttac
      421 tgatgccaat gcaaatttag atacaaaaaa actaaagcat aggataatct ttaaaaaatt
      481 tcgccctagg ataataaaca taacattttg cttcatctaa tttaattaat aatgtagaag
      541 aatcagggaa ttgaatttct ataaaatctc tatcataacg atgaaagaaa ttcctaaaaa
      601 tatcaaatga aaacgaagtt ttaaaaaccc cttttctaat caattcccta taataaatta
      661 acgcttgtgg taaatctcta taaatatgtg aatcagtttg aaatttaact aaatgatatt
      721 cagaaataaa cataattcgg accttttaaa acactctaaa aagccgattc taccaaccgt
      781 taccaaaaca gccaagcaac agacaaagcc aagaagaaac c
//
LOCUS       DAWHRS010000267          789 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWHRS010000267 DAWHRS010000000
VERSION     DAWHRS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.267"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(1..135)
                     /locus_tag="WIB82_002107"
                     /pseudo
     CDS             complement(1..135)
                     /locus_tag="WIB82_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(172..663)
                     /locus_tag="WIB82_002108"
     CDS             complement(172..663)
                     /locus_tag="WIB82_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3843347.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEH
                     TIRDEADFACHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT          209 a          188 c          175 g          217 t
ORIGIN      
        1 cttgtcctga tttaaattta atccactata acgggtcttc gataaatatc ggttacgttt
       61 ggtttgcgtt tccgtcagcg gacggttgcg gcaggctttg cgcataatgc cgtcctgcag
      121 ccgatgctct ttcagttttc cgtaggtcgg attctcgaat ccgacattac ttcaatcata
      181 tccaatagaa aagtccgcat tgccgccacc ccaattatgc ggataaatac cctgtttaac
      241 ataacggtga aacgtagaaa acccccaatc ggaaatttgt cctacatagc catgtttgac
      301 cggattgaaa tgcagataat caaaatggca ggcaaaatcg gcctcatcgc ggatagtatg
      361 ttcccaaaag cgtttttgcc aaagcctgag attgccgccg attaaatatt ggctgtgccg
      421 cttgatttgc cgccagcgtt ccgaataagc agaatcattg tccggcagcc gccatatggt
      481 atgcagatgg tcgggcatca acacccatgc caaaatttca aacggatacc gttcgcgcac
      541 cgccattacc gcctgccgta aagccaaacg caccgcatca tcggtcaaaa tcttctgccg
      601 tttattggtt acaaccgtaa aaaagtaagt gccgccattg cggtaaaaac gacggtattt
      661 catagtatta tgctcggaat gattttgtag gtcggattct tgaattcgac attttgggca
      721 ttgctgcaat ggattgcaat gatgggaatg ttaaaggttt tgtcggatac aagtatccga
      781 cctacgctt
//
LOCUS       DAWHRS010000268          789 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWHRS010000268 DAWHRS010000000
VERSION     DAWHRS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.268"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            5..237
                     /locus_tag="WIB82_002109"
                     /pseudo
     CDS             5..237
                     /locus_tag="WIB82_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            296..604
                     /locus_tag="WIB82_002110"
     CDS             296..604
                     /locus_tag="WIB82_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843348.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMGASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK
                     "
     gene            complement(586..789)
                     /locus_tag="WIB82_002111"
                     /pseudo
     CDS             complement(586..789)
                     /locus_tag="WIB82_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          241 a          192 c          176 g          180 t
ORIGIN      
        1 gaaaatgttg aaaacaaaaa gaactcattg cttgaaaaaa gatttaatgg gaatgcgggt
       61 atttcccaca ctttttccat ttatttatcc tcattatagg aagaaaaaca tgaaaaaacc
      121 ctgcttgcac tctcacttgc agccgtgtcc gccgttacgg tagccgatat tcccgagtct
      181 aacggcaaag gcaaagccat catcggcgat aaggaaaaac accgttacca agcatgaaaa
      241 ccttgctttg aaagtccatg gcgaaatccg ccaagatttg ggggacggat gatccctgac
      301 cccttgggcg gatgcatatg cagatttgcg cggcaaaacc aaagtgatga cgacccagat
      361 gggtgcttcc cgcgatgtca gcaaaagcgc caaaggttgg agtgtcggta tcggtctgaa
      421 tgtaggcaaa cagttgaccg acagcgtcgg tctcgagttt gatccatact accgtcacaa
      481 aacaatctac aaaccccgtg agattgtctt ggacggtgac aaaaccaaaa tgggccgctc
      541 caaatccaac gagtacggct tccgcgtagc cgcaacgttc tatagtcaat taaaatcaaa
      601 ataggacagt agtgcatcgt caaatcgggc gtaatcagac aaaacggttc gcagataccg
      661 cttaatattc gcccacactt tctcaatcgg gttgagctca ggtgaataag gtgcaagagg
      721 caatacctta tgtcccaatt tttccgccat ttcccgtaag acacccatac ggtgaaatca
      781 cgcattatc
//
LOCUS       DAWHRS010000269          781 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWHRS010000269 DAWHRS010000000
VERSION     DAWHRS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.269"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..305
                     /locus_tag="WIB82_002112"
     CDS             <1..305
                     /locus_tag="WIB82_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEZ3843349.1"
                     /translation="QEVELKAGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLV
                     IGGVSAGRERLVETIELPNHPWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            320..522
                     /locus_tag="WIB82_002113"
                     /pseudo
     CDS             320..522
                     /locus_tag="WIB82_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            533..>781
                     /locus_tag="WIB82_002114"
     CDS             533..>781
                     /locus_tag="WIB82_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3843350.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDRVIDWQLIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLIT"
BASE COUNT          215 a          229 c          159 g          178 t
ORIGIN      
        1 cgcaagaagt cgaattgaaa gcaggcagcc tcgccgccaa aatctacggc agcggacata
       61 tccgcgaacg ccaccgccac cgctacgaag tcaacaacaa ctacgttcct acactggaac
      121 aggcaggttt ggtcatcggc ggtgtatctg ccggacgcga acgcttggtc gaaaccatcg
      181 aactgccgaa ccatccttgg ttcttcgcct gccagttcca ccccgagttt acctccaatc
      241 cgcgcaaagg gcatcctttg ttcaccgcgt ttgtcaaagc tgcgttgaac aataaaaaag
      301 cctgataaag cgttacttga tgatcaaaat gccgtctgaa agcctgaatg aagactttca
      361 gacggcattt tcgcaaatcg gggattataa aagccatcaa tttaattttt atggttttat
      421 ctaatattta ccaactgaat attgttaagc ggggcgtgaa actcaagttg agacctttgc
      481 aaaattcccc aaaatcccct aaattcccac caagacattt aggagatttc tcatgagcac
      541 cttcttccag caaaccgccc aagccatgat tgccaaacac atcgaccgct tcccgctatt
      601 gaagttggac cgggtgattg attggcagct gatcgaacaa tacctgaacc gtcaaaaaac
      661 ccgttacctt agagaccacc gcggccgtcc tgcctatccc ctgctgtcca tgttcaaagc
      721 cgtcctgctc ggacaatggc acagcctctc cgatcccgaa ctcgaacaca gcctcatcac
      781 c
//
LOCUS       DAWHRS010000270          777 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWHRS010000270 DAWHRS010000000
VERSION     DAWHRS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.270"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            82..>777
                     /locus_tag="WIB82_002115"
     CDS             82..>777
                     /locus_tag="WIB82_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ3843351.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTYTFYGT"
BASE COUNT          208 a          245 c          183 g          141 t
ORIGIN      
        1 accgtctgaa tacccgatac aaaaatcaga aacgcacaaa caaatcccca ataccccccc
       61 cgttccgaca ggagaccgac cgtgaacact actcctatcc actccaaact cgccgaaatc
      121 accgggcgca ttattgaacg cagccgtccg acgcgtgaaa aatatctggc gaagatccgc
      181 agtgccaaac aaatgggacg cttagagcgc aaccagctcg gctgcagcaa cttggcacac
      241 ggctatgctg ccatgcctaa aagtatcaaa atcgaaatgc ttcaggaaac cgtccccaac
      301 ttaggcatca tcaccgccta caacgacatg gtttccgcac accagccgtt taaagacttc
      361 cctgaccaaa tcaaagacga agcgcagaaa aacggcgcga ccgcccaagt cgccggcggc
      421 acgcccgcca tgtgcgacgg catcacgcaa ggctacgccg gcatggaatt gtcgctgttc
      481 tcccgcgacg tgattgccat gagtaccgcc atcgggctgt cgcatcaaat gtttgacggc
      541 agcctgttta tgggcgtatg cgacaaaatc gttccaggtt tgatgatagg cgcgctttcg
      601 ttcggtcata ttccgggtat cttcgtcccc gcaggcccga tgtccagcgg tatcggcaac
      661 aaagaaaaag cccgcacccg ccagcttttc gccgaaggca aggtcggacg caacgaactt
      721 ttgaaaagcg aaatgggttc ttaccacagc ccgggcacct acactttcta cggcacg
//
LOCUS       DAWHRS010000271          763 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWHRS010000271 DAWHRS010000000
VERSION     DAWHRS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.271"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          238 a          216 c          154 g          155 t
ORIGIN      
        1 ctcaaaccgt cattcccgca aaagcgggaa tccagccccc taacgcaaca ggaatccatc
       61 gaaaaaaccg aaacctccaa accgtcattc ccacggaagt gggaatccag taaccgaaaa
      121 accacaggaa tctatcgaaa aaaaacccaa cccccaaaaa aaccgggcgg atacctcacc
      181 atcctcccaa accctgattt atagcggatg aacaaaaatc agggcaagac ggcgaaaccg
      241 cagacagtac aaatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt
      301 tctctttgag ctaaggcgag ccaacgatgt actggttttt gttaatccac tatatccgtt
      361 tgcgcccgcc aagccgtgtt gcacagatgc gacacggctt ttttctttct gttgtcagaa
      421 tgtaaatttt cgtcaaaaaa catcaaaaat cacccccccc ccgaatttgc gtaaaataaa
      481 gtgtaatccc tttgatcata agggaatgtt gacaattaga tcgattctcc taggctgact
      541 ttcgcaataa atggggttgt caataattgt gtatggtcgt ttgccgtccg gcggccgtca
      601 ttcccgcgca ggcgggaatc cagaaacgca acgcagcagg actttatcgg aaaaaaccga
      661 aatcccaccg ccgtcattcc cgcgcaggcg ggaatccagc aacacaacgc agcaggactt
      721 tatcggaaaa aaccgaaacc ccaccgaccg tcattcccgc aaa
//
LOCUS       DAWHRS010000272          756 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWHRS010000272 DAWHRS010000000
VERSION     DAWHRS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.272"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            299..718
                     /locus_tag="WIB82_002116"
     CDS             299..718
                     /locus_tag="WIB82_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3843352.1"
                     /translation="MDTKHLPSTCRDAASAVCTKTPEYYPNHGEWPSDNSAAGVASSS
                     TIKGKYVEKVEVKNGVITAEMKSSGVNKEIQGKKLSLWAKRQDGSVKWFCGQPVTRNA
                     ANAKAGTDEVTAATPDTDKINTKHLPSTAPTRKSTPN"
BASE COUNT          235 a          230 c          168 g          123 t
ORIGIN      
        1 agattttgcg ttctggattc ccactttcgt gggaatgacg ggatttaatg atgccgccgg
       61 cgtggcatcc tccccctccg acatcaaagg caaatatgtt caaagcgtta cagtcgcaaa
      121 cggcgtcatt accgcccaaa tggcttcaag caacgtaaac aaagaaatca aagacaaaaa
      181 actctccctg tgggccaagc gtcaagacgg ttcggtaaaa tggttctgcg gacagccggt
      241 tcagcgcgcc gccaaagcca aagacgacgt taccgccgac accaccacca acaccaagat
      301 cgacaccaag cacctgccgt caacctgccg cgacgcagca tctgccgttt gcacaaaaac
      361 acccgagtat tacccgaatc acggcgaatg gccgtccgac aatagtgccg ccggcgtggc
      421 atcttcttca acaatcaaag gcaaatatgt tgagaaagtt gaagtcaaaa acggcgtcat
      481 taccgccgaa atgaaatcaa gcggcgtaaa caaagaaatc caaggcaaaa aactctccct
      541 gtgggccaag cgtcaagacg gttcggtaaa atggttctgc ggacagccgg ttacgcgcaa
      601 cgccgccaac gccaaagccg gcaccgacga agtcaccgcc gccacacccg acaccgacaa
      661 aatcaacacc aagcacctgc cgtcaacagc accgacaaga aaatcgacac caaactagag
      721 aaaccttttt acccgataag tttccgtacc gacaag
//
LOCUS       DAWHRS010000273          736 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWHRS010000273 DAWHRS010000000
VERSION     DAWHRS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.273"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            504..>736
                     /locus_tag="WIB82_002117"
     CDS             504..>736
                     /locus_tag="WIB82_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3843353.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGTWPSDNSAAGVASSST"
BASE COUNT          249 a          204 c          116 g          167 t
ORIGIN      
        1 ccatccgcac ggaaacctgc cgcgatgatt caactgccgt ttgcacagga acaccacgcg
       61 ccgatttcaa acactttcca agaaaacgga aatttttaaa aattaaattt aaaatttccc
      121 acccaaccca ccctatgcta ccgcgaaaat tcaaaaatct caaattccga cccaatcaac
      181 acacccgatg ccccatgcca atagagatac cccacgccaa taaaaaagtg ccgaaaatcg
      241 gcactaaaac tgacaatttt caacactgct gacaaaaaac ggtaatcccc tgcttcctca
      301 aaccacaccc aaacgaaaga aaatgtaaaa taaaaacaac catataaaga taaacgcata
      361 aattttcacc tcaaaacaca aacttggtac caatcttgct ttataataca caggtatcgc
      421 aaaaaaatac cgatggtcaa atacattgca taatgccgat ggcgtaagct tgaagcattt
      481 cctttccaat taggagtaat tttatgaaca cccttcaaaa aggttttacc cttatcgagc
      541 tgatgattgt gattgccatc gtcggcattt tggcggcagt cgcccttcct gcttatcaag
      601 actacacagc ccgcgcacaa gtttccgaag ccatcctttt ggccgaaggt caaaaatcag
      661 ccgttaccga gtattacctg aatcacggca catggccgtc cgacaatagt gccgccggcg
      721 tggcatcttc ttcaac
//
LOCUS       DAWHRS010000274          722 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWHRS010000274 DAWHRS010000000
VERSION     DAWHRS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.274"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(140..424)
                     /locus_tag="WIB82_002118"
     CDS             complement(140..424)
                     /locus_tag="WIB82_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ3843354.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          222 a          187 c          117 g          196 t
ORIGIN      
        1 tataaacttt gaatattgcc gttatcccaa ggtctagatt cccgcctacg cgggaatgac
       61 ggtttagaag ttgcccgaaa cctcaaaaaa aaaaccgaaa ccgaacaagc cggattcccg
      121 cctgcgcggg aatgacgggc taaataatat caaaccataa atcctgccaa gaaacattat
      181 tttcttcaat cagttgcaat ttccaagccc tgttccattt cttcaactgt ttttcccgag
      241 taattgcact ctccatcgta ggatgcagtt cataccaaac cagcatagta acgttgtacc
      301 gtgatgtaaa tccctcaatc aaatgctccc tatgttggta aatacgttgc accaaatcag
      361 atgtaacgcc aatgtataac gtgccattac gttggcttgc taaaatataa accgcaggct
      421 gcatataata cccttttgaa ttatttcaat ttatattccc gcgaacacca tcccgtgatt
      481 actttaaccc ttcgttattc ccatagcttt ccatcattcc cgcaactctt cgtcattccc
      541 gcgaaagtgg gaatctagaa cgcaaaatct aaagaaaccg ttttacccga taagtttccg
      601 caccgacaaa cctagattcc cgcctgcgcg ggaatgacgg cggagcggtt tctgtttttt
      661 ccgataaatt cctaaaactc aaaatttcat cattcctaca aaaacagaaa accaaaatca
      721 ga
//
LOCUS       DAWHRS010000275          706 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWHRS010000275 DAWHRS010000000
VERSION     DAWHRS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.275"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(90..641)
                     /locus_tag="WIB82_002119"
     CDS             complement(90..641)
                     /locus_tag="WIB82_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843355.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          198 a          277 c          134 g           97 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggc agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccgaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaacaaggg gctgtcctag ataactagga caaact
//
LOCUS       DAWHRS010000276          696 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWHRS010000276 DAWHRS010000000
VERSION     DAWHRS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.276"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(126..671)
                     /locus_tag="WIB82_002120"
     CDS             complement(126..671)
                     /locus_tag="WIB82_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ3843356.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAASKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
BASE COUNT          161 a          202 c          159 g          174 t
ORIGIN      
        1 cacgcccgac ttacccgggc gcatagacgg atgttgtttc aaatagccaa tattggcagg
       61 cttaatctca aattcaggca tctgcggttt caagacagac agagccaaag caatcaaaga
      121 ctttttcata ccttcgccgc ccccaattcc atcgcaatcg cgtccgcaat cgcgcggcag
      181 atgccccatt tagtagtctt aaacaaggct aaatcagtgt cgttgctgat aaaaaacggc
      241 tcaaacacaa tgccgccggc ttgtgcataa gctaggcgcg aatgctggcc cgcattgtcg
      301 ggtttaaagc cgtcttcgcc gcgcagtttc cagccggttt tcttggcaac agctttgctc
      361 aacacctgac accagcgttt gtttttaacg gtactcaacg cctcaatgcc cgtagctgct
      421 ttactggccg ccgagttggt atggaactca atcgccacat ccgagccgcg aatcagtttt
      481 acagcttcac gcagcggcat attgcctttg cctgtgccgt cggttttaac agtcaaaccg
      541 tagtcatcgc gcaaaatagc agccacaatg ttgcgcatat cctgcgccaa gtccgcctca
      601 cggtcgctgc cgttgaccgc acccgggtcg gtgttgctgt gtccagcggt taaggttacg
      661 gttttgccca ttcatcatct ccaaaaaata ttgatt
//
LOCUS       DAWHRS010000277          675 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWHRS010000277 DAWHRS010000000
VERSION     DAWHRS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.277"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..200
                     /locus_tag="WIB82_002121"
     CDS             <1..200
                     /locus_tag="WIB82_002121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843357.1"
                     /translation="DSGNIQRLSENLEVLDSRFRGNDEKLRESRTSGNGNIQKPSENL
                     KVLDSRFRGNDEVSGGIARLF"
     gene            534..609
                     /locus_tag="WIB82_002122"
     tRNA            534..609
                     /locus_tag="WIB82_002122"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:568..570,aa:Glu,seq:ttc)
BASE COUNT          171 a          170 c          150 g          184 t
ORIGIN      
        1 gggatagcgg caatattcaa aggttatctg aaaatttaga ggttctagat tcccgctttc
       61 gcgggaatga cgaaaagttg cgggaatcca gaacgtcggg caacggcaat attcaaaagc
      121 cgtctgaaaa tttaaaagtt ctagattccc gctttcgcgg gaatgacgaa gtttcaggcg
      181 gcatcgcccg cctgttttga tatggcggca cccccccgac aaaaaaacaa tccggaacgc
      241 atctgaccgt tccggcttgt tttcaggcga atccgccgca tcagaacata ctgcgcacgc
      301 ccatattgac ctgccaagtc tagcgcatcg tgtgcatcga agacctttgc gcctcaaaat
      361 aaagctgcct tccgttgtcg gcattaccac gcaaaaaaat gaattgcttg atattccaat
      421 gttttttata tgtttttata ttgtgatgcg atcagacaaa cgcccccctg acatttgttt
      481 agacggcatc gtattgctaa atttctataa gtatgtataa tgtccgtttc cacgcgccca
      541 tcgtctagag gcctaggaca ctgccctttc acggcggcaa ccggggttcg aatccccgtg
      601 ggcgtgccaa ttcaaaaacc tgcttgtttc aagcaggttt tttattatga gtcgtcattc
      661 ccgcaatttt tcgtc
//
LOCUS       DAWHRS010000278          651 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWHRS010000278 DAWHRS010000000
VERSION     DAWHRS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.278"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WIB82_002123"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WIB82_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ3843358.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          194 a          212 c          116 g          129 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaacaccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aggactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca c
//
LOCUS       DAWHRS010000279          584 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWHRS010000279 DAWHRS010000000
VERSION     DAWHRS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.279"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          131 a          130 c          165 g          158 t
ORIGIN      
        1 atcctgcggg attggtgtgg aatttagggc taatctagta cagccccaaa atttaaatca
       61 ggacaaagcg acgaagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt
      121 cagcacctta gagaatcgtt ctctttgagc tagggcgaac caacgctgta ctggtttaga
      181 tttaattcac tatacctgcg taatggtatc gggtactcat catcaacctg ccttgcattc
      241 ggttgtgttg tccggcaact gtccggttgt gtcgatgggt tgccctgccg ctccctgtgc
      301 tgcgcaacgg ttgtgtgcct tgggtgggcg cgggtggcgg ccgggcggtt tgttgctatg
      361 atacgggagt tccgatatac aagggtgggc ttcagcccac caatcaacga ccgaaaaaga
      421 aaaaacgttg ggctgaagcc cactctacgc aattacgcaa ctgttatcgt ggggatgacg
      481 ggattttagg tttttgtttt tctgtttttg cgggaaggat gggttttagg ttgcggtcat
      541 ttatcggaaa agcagaaatc gctccgccgt cattcccgcg aaag
//
LOCUS       DAWHRS010000280          581 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWHRS010000280 DAWHRS010000000
VERSION     DAWHRS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.280"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..118)
                     /locus_tag="WIB82_002124"
     CDS             complement(<1..118)
                     /locus_tag="WIB82_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ3843359.1"
                     /translation="MRKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREET"
     gene            177..398
                     /locus_tag="WIB82_002125"
     CDS             177..398
                     /locus_tag="WIB82_002125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843360.1"
                     /translation="MLSTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQAEI"
BASE COUNT          142 a          175 c          131 g          133 t
ORIGIN      
        1 gcgtttcctc acgttgcgcg ccggcgtgcg ccagaggact gagcaacaac gcaccggcgg
       61 cacacaacag gcggcggcgt tgcagattaa cgggtagggt atcggtcggt tttctcatag
      121 ggaacggggg cgcgtccgga cgtttcagac ggcattaaat attcaaacag acataattgc
      181 tttcaacgcg aaaaaccgcg cgcaaaatcc aagcgcggca tatcgccctg cccttttcgg
      241 gcaaacctca attctaccgc cctcaagaac gcttgtccaa acaggcacag gcaacaccgc
      301 ccgggcattt ccgttttcac cggttatccg tcgtccggat tatgcagcag caccatcagc
      361 gcatcacgct tttcgggcgg cagcaggcgg aaatatagta gattaaattt aaaccagtac
      421 agcgttgcct cgccttgccg tactgattta aatttaatcc actatatctt gaggcctttg
      481 caaaattcct ttccctcccg acagccgaaa cccaaacaca ggttttcggc tgttttcgcc
      541 ccagatacct cctaatttta cccaaatacc cctttaatcc t
//
LOCUS       DAWHRS010000281          567 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWHRS010000281 DAWHRS010000000
VERSION     DAWHRS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.281"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(452..567)
                     /locus_tag="WIB82_002126"
                     /pseudo
     CDS             complement(452..567)
                     /locus_tag="WIB82_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          135 a          111 c          145 g          176 t
ORIGIN      
        1 gggaatgacg ggattaaagt ttcaaaattt attctaaata actgaaactc aacgaactag
       61 attcccactt tcgtgggaat gacgaatttt aggtttctgt ttttggtttt ctgtttttgt
      121 aggaatgatg aaattttaag ttttaggaat ttatcggaaa aaacagaaac cgctccgccg
      181 tcattcccgc gcaggcggga atccagcctc gtcggtgcgg aaacttatcg ggaaaacggt
      241 ttctttagat tttacgttct ggattcctac tttcgtggga aagacgaatt ttaggtttct
      301 gtttttggtt ttctgtcctt gtgggaatga tgaaaattta agttttagga atttatcgga
      361 aaaaacagaa accgctctgc cgtcattccc gcaaaagcgg gaatccagcc tcgtcggtgc
      421 ggaaacttat cgggtaaaaa ggtttcttta gtttggtgtc gattttgtcg gtgccggtgg
      481 cggcggcaac gtcgtctttg gcgttggcgg cgcgcgtaac cggctgtccg cagaaccatt
      541 ttaccgaacc ggcttgacgc ttggccc
//
LOCUS       DAWHRS010000282          566 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWHRS010000282 DAWHRS010000000
VERSION     DAWHRS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.282"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            21..>566
                     /locus_tag="WIB82_002127"
     CDS             21..>566
                     /locus_tag="WIB82_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ3843361.1"
                     /translation="MQADLAYAAERITHDYPKATGADKDKISTVSDYFRNIRAHSIHP
                     RVSVGYDFGGWRIAADYASYRKWNNNKYSVNTKKVQENQGTRKDLKTENQENGSFHAA
                     SSLGLSAIYDFKLNDKFKPYIGARVAYGHVRHSIDSTKKTTEVVTSTHGGADTKPTIY
                     NGESTQNAYHESHSIRRLGLGV"
BASE COUNT          178 a          151 c          126 g          111 t
ORIGIN      
        1 gcgggcacgg cccgtattat gtgcaggcgg atttagctta tgccgccgaa cgtattaccc
       61 acgattatcc gaaagcaacc ggtgcagaca aagacaaaat aagcacagta agcgattatt
      121 tcagaaacat ccgtgcgcat tccatccacc ctcgtgtgtc ggtcggctac gacttcggcg
      181 gctggagaat agcggcagat tatgccagtt acagaaaatg gaacaacaat aaatattccg
      241 tcaacacaaa aaaggtgcaa gaaaaccaag gcaccagaaa agacctgaag acggaaaatc
      301 aggaaaacgg cagcttccac gccgcttctt ctctcggctt atccgccatt tacgatttca
      361 aactcaacga taaattcaaa ccctatatcg gtgcgcgcgt cgcctacgga cacgtcagac
      421 acagcatcga ttcgaccaaa aaaacaacag aggttgttac ctccacccat ggtggtgctg
      481 acacaaaacc tacgatttat aatggggaaa gtacgcaaaa cgcctatcac gaaagccaca
      541 gcatccgccg cttgggtctt ggtgtc
//
LOCUS       DAWHRS010000283          562 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWHRS010000283 DAWHRS010000000
VERSION     DAWHRS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.283"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..525
                     /locus_tag="WIB82_002128"
     CDS             <1..525
                     /locus_tag="WIB82_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HEZ3843362.1"
                     /translation="NRLHSTEEDFIRNIHQDLIDTIQDKMEQVKIAISEQIKKQTDNN
                     HYRNLQTIPSIGKDTASVLYAQLTEKHFKTANQFVSYAGLSPAIIQSGTSVRGRGRLS
                     RYGNRRLKSTLYMPALCAYRFNAFPKLINNLKKAGKPKMVIIVAIMRKLAKLAYYIVK
                     TGQPYDAERHRLNQ"
BASE COUNT          209 a          127 c          105 g          121 t
ORIGIN      
        1 aaccgtcttc atagcactga agaagacttc ataaggaaca tacatcaaga cttgatagat
       61 accatacagg acaagatgga acaggtaaaa atagccatat ccgaacaaat caaaaaacaa
      121 acggacaata accattaccg caatcttcaa accatcccga gcataggcaa agacaccgca
      181 tcagttcttt atgcgcaact gacagaaaaa cattttaaaa ccgcaaacca gtttgtatcc
      241 tatgccggat taagtcccgc catcatacaa tcagggacaa gcgtaagagg tcggggcaga
      301 ttgagccgat acggaaacag acgattaaaa agtacgctgt atatgcccgc cctttgtgct
      361 taccgtttta acgcatttcc gaaattaata aataatctga aaaaagcggg taagccaaag
      421 atggtaatca tcgttgccat catgcgcaaa ctggcgaagc tcgcctatta cattgttaaa
      481 accggccagc cttacgatgc ggaaagacac cgattgaatc aataaaattc aacaaaatta
      541 aacggttacg cgaatatatt tg
//
LOCUS       DAWHRS010000284          530 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWHRS010000284 DAWHRS010000000
VERSION     DAWHRS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.284"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            32..516
                     /locus_tag="WIB82_002129"
                     /pseudo
     CDS             32..516
                     /locus_tag="WIB82_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           71 a          105 c          212 g          142 t
ORIGIN      
        1 atgcggtctg cttcggaaga gggggacggc gatgtttgtg aacgagaaat atccttatgc
       61 ggctctgttt gcgggactgg tgtttttgac gctgccgttt gcgttggcgg tgcatgatgc
      121 ctttgcgctt gcgttcggac ggacggggtt gctggtgtcg gtgtcggacg gcggattcgg
      181 ctggcgtggc ggttgggacg gcactgtttg gtttgtgttc ggtgtgtttg cgtttttgaa
      241 tgtggttgtg tcggcgggtc tgacgaaact ggcgtacaaa aagatgatgc ggcggcattc
      301 gcgttacaca ctgtttctgt cgggcgtggc ggcttgcgcg gcggcagcgg tggcttggat
      361 tttcgagctg ctgcttggca gtggggcttt gggcgggctg cgggggaggc ggtgttggaa
      421 tatgcgtttg ccgtgtggct ggtggcgatg ctgacgctgc ccaaacgcct gacgcgcgcg
      481 ccggtgcagc cggtggtgtt tcacaggaaa aaataggttg gaacgggaaa
//
LOCUS       DAWHRS010000285          523 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWHRS010000285 DAWHRS010000000
VERSION     DAWHRS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.285"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          144 a          110 c          133 g          136 t
ORIGIN      
        1 aaaaaaccct ccgccgtcat tcccgcgaaa gcgggaatct agaattttaa tgcggcaaga
       61 atttatcaag aaaaaccgaa gcttaaagac ctagattccc gcctgcgcgg gaatgacggt
      121 ttagaaatca cccgaaacga gaaaaagaac cgaaaccgaa caagccggat tcccgcacag
      181 gcgggaagga cggaatttca gattgttgtt tttgattttc tgtttttgtg ggaataacgg
      241 gactttggat gatgtgcatt tatcgagaaa actaaaaacc ctccgccgtc attcccgcga
      301 aagcgggaat ctagaccttt cggtgcggaa acttatcgga taaaacggtt ttccctgcat
      361 cctggattcc cactttcgtg cgaatgacgg gatataggtt tcactacgga cgtgttcaga
      421 ttaccgcctg cgcgggaagg acgggatttc aggttgctgt ttttgatttt ctgtttttgt
      481 gggaataacg ggactttgga tgatgtgcat ttatcgggaa aac
//
LOCUS       DAWHRS010000286          499 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWHRS010000286 DAWHRS010000000
VERSION     DAWHRS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.286"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..137)
                     /locus_tag="WIB82_002130"
     CDS             complement(<1..137)
                     /locus_tag="WIB82_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ3843363.1"
                     /translation="MSTFFQQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YL"
BASE COUNT          125 a           94 c          124 g          156 t
ORIGIN      
        1 aggtaacggg ttttttgacg attcaggtat tgttcgatcg gctgccaatc aatcacctgg
       61 tccaacttca atagcgggaa gcggttgatg tgtttggcaa tcatggcttg ggcggtttgt
      121 tggaagaagg tgctcatgag aaatccccta aatgtcttgg tgggaattta ggggattttg
      181 gggaattttg caaaggtctc aaaccggttt gtcagggttt aatggtcagc gcgtcctgcg
      241 ttgccatttg ttgtagtagt attcgtaatc ttcatcacga ccgcccgatt cgatccaatt
      301 atcgtattcg gggctgtttt caaagctgtc agacattatc cgtacctcgc aaaatgggtt
      361 tatcgatact tttttagtta tggaatcaac cataactaaa aagaagtata cttctttgct
      421 tttccgccaa aatatttaac cgcacatccg ctgcagggta aaaaatggaa gttcaacgat
      481 attgatacgg gtatgggcg
//
LOCUS       DAWHRS010000287          498 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWHRS010000287 DAWHRS010000000
VERSION     DAWHRS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.287"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(1..133)
                     /locus_tag="WIB82_002131"
                     /pseudo
     CDS             complement(1..133)
                     /locus_tag="WIB82_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(217..>498)
                     /locus_tag="WIB82_002132"
     CDS             complement(217..>498)
                     /locus_tag="WIB82_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843364.1"
                     /translation="IYSRQGESLIGIGIREAALPVPTAIDILNLFINERILVLGGDIY
                     IKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
BASE COUNT          146 a           91 c           79 g          182 t
ORIGIN      
        1 tggcagagtt gataccgtct tttaccgcaa aagcactgtc ttgtaatgcg gttgcagccg
       61 caagttgtgc ttcgggactt aaatttccta aatgattaat accttgtaga gtctgctgag
      121 cggctgtatc tgagccggtc agtgtaggtt ggattaaaac ccagcatttt caacatcttg
      181 atgttttact ataaatctat tcttgtgctt cctttgttag ataaatttca acacaaaaga
      241 tacgtatgca ttctctaatt ttatttgaga taaataatgc attgctttgt tgatactgtt
      301 aaataaatta cttccctcgt aataccaatt atcatatgtt tgataaaaat aattatcttt
      361 cttgatataa atatctcccc ccaatacaag tattctttca tttataaata aatttaatat
      421 atctattgca gtggggacgg gtaatgcggc ttctctaatt ccaattccta tcaaagattc
      481 cccttgtctc gaataaat
//
LOCUS       DAWHRS010000288          495 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWHRS010000288 DAWHRS010000000
VERSION     DAWHRS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.288"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..>495)
                     /gene="mafB"
                     /locus_tag="WIB82_002133"
     CDS             complement(<1..>495)
                     /gene="mafB"
                     /locus_tag="WIB82_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ3843365.1"
                     /translation="EDGYDGPQGSDYPPPGGARDIYSYYVKGTSTKTKTNIVPQAPFS
                     DRWLKENAGAASGFFSRADEAGKLIWENDPNQNWWGNRMDDVRGIIQGAANPFLTGFQ
                     GLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLSPEAQLAAASLLQDSAFAVKDSINS
                     ARQWA"
BASE COUNT          109 a          128 c          110 g          148 t
ORIGIN      
        1 agcccattgt ctggcagagt tgatactgtc ttttaccgca aaagcactgt cttgtaatag
       61 gctcgcggcc gcaagttgtg cttccggact taaatttcct aaattgtgaa taccctgtaa
      121 agttttccgt gctgccgcat aggttaccgg gtttaccgca ctgtctgtaa ttgccccaac
      181 tcccaatccc tgaaaacccg ttagaaaagg attggctgca ccttggatga tgccgcgaac
      241 atcatccata cggttacccc accaattttg attggggtcg ttttcccata tcagttttcc
      301 tgcttcatcc gcacggctga aaaaaccaga ggcggcaccg gcattttctt ttagccaacg
      361 gtctgaaaat ggggcttgag ggacaatatt agtctttgtt tttgttgaag ttcctttgac
      421 ataatagctg tatatatccc ttgctcctcc ggggggcgga taatcgctgc cttgcggtcc
      481 gtcatatcca tcctc
//
LOCUS       DAWHRS010000289          495 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWHRS010000289 DAWHRS010000000
VERSION     DAWHRS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.289"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            16..342
                     /locus_tag="WIB82_002134"
     CDS             16..342
                     /locus_tag="WIB82_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ3843366.1"
                     /translation="MGEKIKDGKNQKTVLSREEEQKICNTFTHKQAVEDFSVVIGYDE
                     IKAKNHSLSAWQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLA
                     MLKFNS"
     gene            353..490
                     /locus_tag="WIB82_002135"
     CDS             353..490
                     /locus_tag="WIB82_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ3843367.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKEKIQKTKATGIYR
                     K"
BASE COUNT          176 a           91 c          111 g          117 t
ORIGIN      
        1 atcgacgcat cgggcttggg cgaaaaaatc aaagacggca aaaaccaaaa aaccgtactt
       61 tctcgcgaag aagaacaaaa aatctgcaat actttcacgc acaaacaagc agtggaagat
      121 ttcagcgtgg tgatcggcta cgatgaaatc aaagcgaaga atcacagctt gtcggcgtgg
      181 cagtatttcg aggtaaagat tgattatgtg gatatttccg ccgacgaatt tgcgcaaaaa
      241 atggcgggat tttcagcgga tttggataaa cttttcgccg aatctgccga attggaaaaa
      301 gagattaagg atagattggc gatgttgaaa ttcaattcat aaataccgat gaatgaaacc
      361 ggtcaaactt aaaatgccgt ctgaaagcct ttcagacggc attttgtttg gtttggaaaa
      421 tacttatgcc gttctttccc tgaaagagaa aatccaaaaa accaaagcca caggaattta
      481 tcggaaatga caaaa
//
LOCUS       DAWHRS010000290          477 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.290, whole genome shotgun sequence.
ACCESSION   DAWHRS010000290 DAWHRS010000000
VERSION     DAWHRS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.290"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>477
                     /locus_tag="WIB82_002136"
     CDS             <1..>477
                     /locus_tag="WIB82_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3843368.1"
                     /translation="EQYLNRQKNRYLRDHRGRPAYPLLSIFKAVLLGQWHSLSDPELE
                     HSLITRIDFNLFCRFDELSIPDYSTLCRYRNRLAQDNTLSELLELINRQLTEKGLKIE
                     KASAAVVDATIIQTAGSKQRQAIEVDEEGQISGQTTPSKDSDARWIKKNGLYKLGYK"
BASE COUNT          139 a          149 c          102 g           87 t
ORIGIN      
        1 gagcagtacc tgaaccgtca aaaaaaccgt tacctccgag accaccgcgg ccgtcccgcc
       61 tacccgctgc tgtccatatt caaagccgtc ctgctcggac aatggcacag tctctccgat
      121 cccgaactcg aacacagcct cattacccgc atcgatttca acctgttttg ccgtttcgac
      181 gaactgagca tccccgatta cagcacctta tgccgctacc gcaaccggct ggcgcaagac
      241 aataccctgt ctgaactgtt ggaactgatt aaccgccaac tgaccgaaaa aggtttaaaa
      301 atagagaaag catccgctgc cgtcgttgac gccaccatta ttcagaccgc cggcagcaaa
      361 cagcgtcagg ccatagaagt tgacgaagaa ggacaaatca gcggtcaaac cacaccgagt
      421 aaggacagcg atgcccgttg gataaagaaa aacggcctct acaaactcgg ttacaaa
//
LOCUS       DAWHRS010000291          476 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.291, whole genome shotgun sequence.
ACCESSION   DAWHRS010000291 DAWHRS010000000
VERSION     DAWHRS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.291"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          128 a          134 c          100 g          114 t
ORIGIN      
        1 tgcgcgggaa tgacggcgga gggtggacga tgccgtctga aacttcgcca ttcccatgat
       61 taccgcaacc tttcgtcatt cccgccacct ttcgtcattc ccgtgaaaac gggaatctag
      121 aacctcaaac tttcggataa tctttgaata ttgccgctgc ccgaaggtct ggattcccac
      181 aacctttcgt cattcccgtg aaaacgggaa tctagaacct caaactttca gataaccttt
      241 gaatattgcc gctgcccgaa ggtctggatt cccacaacat ttcgtcattt ccgtgaaaac
      301 gggaatctag aacctctaaa ctttcagata atctttgaat attgccgtcg cccaaaggcc
      361 tggattcccg cctgcgcggg aatgacggtt tagaagttgc ccgaaacctc aaaaaaaacc
      421 gaaaccgaac aagccggatt cccgcaacat tccgtcattc ccgtgaaaac gggaat
//
LOCUS       DAWHRS010000292          476 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.292, whole genome shotgun sequence.
ACCESSION   DAWHRS010000292 DAWHRS010000000
VERSION     DAWHRS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.292"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          131 a          106 c          103 g          136 t
ORIGIN      
        1 tttttttgaa tttcgggcaa cgcttgaatc ttcattccgc gcaggcggaa attatcggtg
       61 cggtacggca acttttttcg atatgaaaag accgtcattc ctgtaaaaac aaaaaatcaa
      121 aaacagaaaa ttgaaattcg tcattcccgc gcaggcggga atccaggacg taaaatctat
      181 agtggattaa caaaaaccag tacagcgttg ccttgactta gctcgaagag aacgattctc
      241 taaggtgctg aagcaccgag tgagtcggtt ccgtactatc cgtactgtct gcggcttcgt
      301 cgccttgtcc tgatttttgt taatccacta taaagaaacc gtttttctcg ataagtttcc
      361 gtgccgacag acctggattc ccactttcgt gggaatgacg gtggaaaagt tgccgtgatt
      421 tcggataaat tttcgtaacg cataatttcc gttttacccg ataaatgccc gcaatc
//
LOCUS       DAWHRS010000293          472 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.293, whole genome shotgun sequence.
ACCESSION   DAWHRS010000293 DAWHRS010000000
VERSION     DAWHRS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.293"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(342..>472)
                     /locus_tag="WIB82_002137"
     CDS             complement(342..>472)
                     /locus_tag="WIB82_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ3843369.1"
                     /translation="QPVTRTDAKAKAGTDEVTAATPDTKIDTKHLPSTCRDESTAS"
BASE COUNT           93 a          102 c          140 g          137 t
ORIGIN      
        1 attttaggtt tctgtttttg gttttctgtc cttgtgggaa tgacgtggtg caggtttccg
       61 tgcggatagc ttcatcattc ccgagtaggc gggaatctag tccgcttgtt cggtaaatga
      121 gagggcggat tgcgcgcctg tcagataaac cacgtgttta aacgggcggc aatgaggtac
      181 gcgcagagcc ttgaagcgca gtcgatatat tattttccac caaaacggac gaccccgctt
      241 gccttgcaaa cctttaaaaa ggaagccacc cggattaatc cgagtggccg tggaaaatca
      301 cttaccgctt gatttattta aaatttatgg tataatttac cttagctggc agttgattca
      361 tcgcggcagg ttgacggcag gtgcttggtg tcgatcttgg tgtcgggtgt ggcggcggtg
      421 acttcgtcgg tgccggcttt ggctttggcg tcggtgcgcg taaccggctg tc
//
LOCUS       DAWHRS010000294          471 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.294, whole genome shotgun sequence.
ACCESSION   DAWHRS010000294 DAWHRS010000000
VERSION     DAWHRS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.294"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          120 a           90 c           98 g          163 t
ORIGIN      
        1 taaccgtagc aactgaaccg tcattcccac gaaagtggga atctagaatc tcagactttc
       61 agataatctt tgaatattgc tgttgttcta aggtctagat tcccgcctta tatgatgcgc
      121 tctatcaaag gggcgcatta cttttcttaa cattcccctt tgacagccaa gtgaaagggg
      181 cttttttatg tcagtagcaa atgtaatatt ttcttgttcc tattggagaa tatttaaaaa
      241 atcagattat tgcgttttat gtttctatca gttcaggcat ggtgaaccgc ataaactcgc
      301 tgaacaagaa aatttttcaa agctttatca ggcgttcgat tatatagatt cggttggctc
      361 gaattttccg gtaattatca caacagacgg ttgtggtctt tcttcttgat ctttaacagt
      421 ttgtcaggat tgggctttcg gtcgttgacc gttggacgcg ctttagcgcg g
//
LOCUS       DAWHRS010000295          460 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.295, whole genome shotgun sequence.
ACCESSION   DAWHRS010000295 DAWHRS010000000
VERSION     DAWHRS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.295"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..114
                     /locus_tag="WIB82_002138"
     CDS             <1..114
                     /locus_tag="WIB82_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ3843370.1"
                     /translation="ARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          110 a          105 c          134 g          111 t
ORIGIN      
        1 gcgcgggcag cctatttcgg actgattaaa gtgagtgcgc aaagccacct gaaggcgatg
       61 tgtttgaacc tgttgaaagc cgccaacagg ctaagtgcgc ccgctgccgc ctaaaaggca
      121 gcccggatgc ctgattatcg ggtatccggg caggattaag ggggtgtttg ggtaaaatta
      181 ggcggtattt ggggcgaaaa cagccgaaaa cctgtgtttg ggtttcggct gtcgggaggg
      241 aaaggaattt tgcaaaggtc tcagattgtt gtcgcttcgg ataaattttt gccgcgttgg
      301 gttctagatt cccgcttttg cgggaatgac ggcagggtgg tttcagttgc tcccgataaa
      361 tgccgccatc tcaagtctcg tcattccctc aaaaacagaa aaccaaaatc agaaacctaa
      421 aatcccgtca ttcccgcgca ggcgggaatc taggtctgtc
//
LOCUS       DAWHRS010000296          449 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.296, whole genome shotgun sequence.
ACCESSION   DAWHRS010000296 DAWHRS010000000
VERSION     DAWHRS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.296"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(115..>449)
                     /locus_tag="WIB82_002139"
     CDS             complement(115..>449)
                     /locus_tag="WIB82_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843371.1"
                     /translation="VDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFY
                     GENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANIQSVQQ
                     PLLVTPSA"
BASE COUNT          148 a           93 c           79 g          129 t
ORIGIN      
        1 gcaccgatta aggttaatta aaatacgacc cttatcccaa tattaagaaa aacagcaatc
       61 aaaataaact tgattgctgt ttttcagttt aagccatgaa tgtgattagg ctccttatgc
      121 agatggcgtt accaataacg gctgctgtac ggattgtata ttggcatcca catttcctcc
      181 tgtcgcagca gtattagaac cgaacacaga catagactgt accaaattat tgccagcatt
      241 aatatacttg gcaaaatctg gattactgat agctgcatca tcaaagacaa atgtatctac
      301 acgatggttt tcaccataga agaatccgga aatacgtacg ttgtcttgtt cagaagcgct
      361 aagcaccaaa tcacttccgg aacggataaa atgaacatct gctgctttaa atcctttaaa
      421 gtgcatagtg tcagagtttt tatccacat
//
LOCUS       DAWHRS010000297          430 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.297, whole genome shotgun sequence.
ACCESSION   DAWHRS010000297 DAWHRS010000000
VERSION     DAWHRS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.297"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>430
                     /locus_tag="WIB82_002140"
     CDS             <1..>430
                     /locus_tag="WIB82_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ3843372.1"
                     /translation="GNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSKLGRL
                     AYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQI
                     KAVAYELLEKHLPEEDFERARRFLYQCFKRTDTPPAGVWL"
BASE COUNT          117 a          112 c          108 g           93 t
ORIGIN      
        1 tcggcaaccc catacacaac aacacgatga ttatgcggcg cagcgtcatt gacggcggtt
       61 tgcgttacaa caccgagcgg gattgggcgg aagattacca attttggtac gatgtcagta
      121 aattgggcag gctggcttat tatcccgaag ccttggtcaa ataccgcctt cacgccaatc
      181 aggtttcatc caaatacagc gtccgccaac acgaaatcgc gcagggcatc caaaaaaccg
      241 ccagaaacga ttttttgcag tctatgggtt ttaaaacccg gttcgacagc cttgaatacc
      301 gccaaataaa agcagtagcg tatgaattgc tggagaaaca tttgccggaa gaagattttg
      361 aacgcgcccg ccggtttttg taccaatgct tcaaacggac ggacacgccg cccgccggcg
      421 tgtggctgga
//
LOCUS       DAWHRS010000298          421 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.298, whole genome shotgun sequence.
ACCESSION   DAWHRS010000298 DAWHRS010000000
VERSION     DAWHRS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.298"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            15..197
                     /locus_tag="WIB82_002141"
                     /pseudo
     CDS             15..197
                     /locus_tag="WIB82_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          127 a           93 c           88 g          113 t
ORIGIN      
        1 taaaaaatat ccctttgaaa gattttgatg aagtttgtga aaagtgggag aaatccttta
       61 aagaaaatgg aaaagaaatt gaatataatc aaaaatattc aagaatttat ggtgaagttc
      121 gtggagctgg ctttaatggc gatatcggag aaaagctaca tcgttgtgcc gtgatggaat
      181 gggctgaaaa tgagaccgag acctttgcaa aattcctttc cctcccgaca gccgaaaccc
      241 aaacacaggt tttcagctgt tttcagctgt tttcgcccca aatatctcct aattctaccc
      301 aaataccccc ttaatcctgc ccggatacct gataatcagg catccgggct gctttttagg
      361 cggcagcggg cgcacttaac ctgttggccg ctttcaacag gttcaaacac atcgccttca
      421 g
//
LOCUS       DAWHRS010000299          420 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.299, whole genome shotgun sequence.
ACCESSION   DAWHRS010000299 DAWHRS010000000
VERSION     DAWHRS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.299"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(140..>420)
                     /locus_tag="WIB82_002142"
     CDS             complement(140..>420)
                     /locus_tag="WIB82_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3843373.1"
                     /translation="EIKGKYVKSVEVKNGVVTAQMASSNVNNEIKGKKLSLWAKRQNG
                     SVKWFCGQPVTRDKAKDDAVTAATGKGTANINTKHLPSTAPTRKSTPN"
BASE COUNT           76 a           87 c          117 g          140 t
ORIGIN      
        1 ttttctgttt ttgagggaat gatgaaattt taagttttag gaatttatca gaaaaaacag
       61 aaaccgctcc gccgtcattc ccgcgcaggc gggaatccag gtctgtcggt acggaaactt
      121 atcgggtaaa aaggtttctc tagtttggtg tcgattttct tgtcggtgct gttgacggca
      181 ggtgcttggt gttgatgttg gcggtgccct tgccggtggc ggcggtgacg gcgtcgtctt
      241 tggctttgtc gcgcgtaacc ggctgtccgc agaaccattt taccgaaccg ttttgacgct
      301 tggcccacag ggagagtttt ttgcctttga tttcgttgtt tacgttgctt gaagccattt
      361 gggcggtaac gacgccgttt ttgacttcaa cgcttttaac atatttgcct ttgatttcag
//
LOCUS       DAWHRS010000300          416 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.300, whole genome shotgun sequence.
ACCESSION   DAWHRS010000300 DAWHRS010000000
VERSION     DAWHRS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.300"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            41..416
                     /locus_tag="WIB82_002143"
                     /pseudo
     CDS             41..416
                     /locus_tag="WIB82_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          128 a          116 c           94 g           78 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat actttatgcc gctaccgcaa
       61 ctggcggcgc aagacgacac cctgtccgaa ttgctcaaac tgattaactg ccaactgacc
      121 gaaaaaggct taaaagtaga gaaagcatcc gccgctgtcg ttgacgccac cattattcag
      181 accgccggca gcaaacagcg tcaggctata gaagtcgatg aagaaggaaa agtcagcggc
      241 caaaccacac cgagtaaaga taaagatgcc cgccggacaa agaaaaacgg cctctacaga
      301 ctcagttaca aacaacatac ccgtaccgat gaggaaggct atatcgagaa actgcacatt
      361 acccccgcca atgcccatga gtgcaaacac ctgtcgccgt tgttggaagg gatagc
//
LOCUS       DAWHRS010000301          416 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.301, whole genome shotgun sequence.
ACCESSION   DAWHRS010000301 DAWHRS010000000
VERSION     DAWHRS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.301"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(96..389)
                     /locus_tag="WIB82_002144"
     CDS             complement(96..389)
                     /locus_tag="WIB82_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3843374.1"
                     /translation="MATSANIKGKYVEKVEVKNGVVTATMLSTGVNNEIKGKKLSLWA
                     KRQDGSVKWFYGQPVKRDNASADAVKADTAANGKQIDTKHLPSTASTRKSTPN"
BASE COUNT           70 a          101 c          121 g          124 t
ORIGIN      
        1 ttatcggaaa aaacagaaac cgctccgccg tcattcccgc gcaggcggga atccagcctt
       61 gtcggtacgg aaacttatcg ggtaaaaagt tttctctagt ttggtgtcga ttttcttgtc
      121 gatgctgttg acggcaggtg cttggtgtcg atctgcttgc cgttggcggc ggtgtcggct
      181 ttgacggcgt cggcgctggc gttgtcgcgc ttaaccggct gtccgtagaa ccattttacc
      241 gaaccgtctt gacgcttggc ccacagggag agttttttgc ctttgatttc gttgtttacg
      301 ccggttgaaa gcattgtggc ggtaacgacg ccgtttttga cttcaacttt ctcaacatat
      361 ttgcctttga tgttggcgga ggttgccacg ccggcagaac tgttgttgcc gggcca
//
LOCUS       DAWHRS010000302          414 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.302, whole genome shotgun sequence.
ACCESSION   DAWHRS010000302 DAWHRS010000000
VERSION     DAWHRS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.302"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          102 a           69 c           85 g          158 t
ORIGIN      
        1 ttcagataat ctttgaatat tactgttgtt ctaaggtcta gattcccgcc ttatatgatg
       61 cgctctatca aaggggcgca ttaattttct taacattccc ctttgacagc caagtgaaag
      121 gggctttttt atgtcagcag taaatgtaat attttcctgt tcttattgga gaatatttaa
      181 aaaatcagat tcttgtgttt tgtgttttta tcagttcaga catggcgaac cgcataaact
      241 cattaatcaa gagaattttt caaagcttta tcaggcgttc gattatatag attcggttgg
      301 ttcgaatttt ccagtgatta tcacaacgga tggttgtggt cttttttgtt gatctttaaa
      361 agtttgtcag gatttggctt tcggtcgttg accgtcgtac gcgctttagc gcgg
//
LOCUS       DAWHRS010000303          413 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.303, whole genome shotgun sequence.
ACCESSION   DAWHRS010000303 DAWHRS010000000
VERSION     DAWHRS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.303"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          112 a          114 c           84 g          103 t
ORIGIN      
        1 gttcccacgg tcaggatttc agattgcgga catctgtcag aaaagacaaa aaaccttccg
       61 ccgtcattcc ctacaggcga gaatccgatc cgttgaaatt cggttgtttt aaataaattc
      121 ttgcagcttt gattttctgt ttttccgata acgccgtaac tttgaaacgc gaaagcggta
      181 atccgatccg ttgggatttt gcaacttcaa atcaatccgc aaactgaaat cccgtcattc
      241 ccgcgcagtc gtgaatccga acgcgtccgc acgaaaacct gcatcccgtc attcccgcgc
      301 agtcgtgaat ccgaacgcgt ccgcacgaaa acctgcatcc cgtcattccc acggaagtgg
      361 gaatctagga cgtaaaatct aaagaaaccg ttttatccga taagtttccg cac
//
LOCUS       DAWHRS010000304          402 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.304, whole genome shotgun sequence.
ACCESSION   DAWHRS010000304 DAWHRS010000000
VERSION     DAWHRS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.304"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          133 a           93 c           67 g          109 t
ORIGIN      
        1 caacgctgta ctggtttttg ttaatccact ataaaaggtc ttttctaaaa tatgcagaaa
       61 ttcgaatatc gaatttgcct tatgattacg gttttctgtc ttatctgcct taaaacgccg
      121 gtattccgtt tgaaccaaat tatatttgcc gaaacgctca aaaatccccc ttacctgccc
      181 gacagacatc aagccttcgt tgttgtagct gagaaaatat attgaaagtc cgcatcccta
      241 atcaaagcct caaataccgg aatcacatgc gattttgagc aagtccgcgc taaaacacat
      301 cgtacagcgc aaaaccggat gggaatggca aaatccggaa acaaacattt ttcaaagcat
      361 acaaacaaaa aaataccaac cggaggttgg tattccttaa ct
//
LOCUS       DAWHRS010000305          402 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.305, whole genome shotgun sequence.
ACCESSION   DAWHRS010000305 DAWHRS010000000
VERSION     DAWHRS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.305"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..338
                     /locus_tag="WIB82_002145"
     CDS             <1..338
                     /locus_tag="WIB82_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843375.1"
                     /translation="LAVSHHQGYKSAFAKQQAVIDKMEKDKAQAMLLSAQNYARELEQ
                     ARAEAKKYEVKAHAVGMALAKKQAEVSRLKTERDLCKIPFPPDSRNPNTGFRLFSPQI
                     PPNFTQIPP"
BASE COUNT          110 a          111 c          101 g           80 t
ORIGIN      
        1 tcttggcggt gtcgcaccat caaggctaca agtcggcttt tgcgaagcag caggcggtca
       61 ttgacaagat ggaaaaagac aaagcgcaag ccatgctgtt gtcggctcaa aactatgcac
      121 gcgagctgga acaggcgcgt gcggaagcga aaaaatatga agtcaaggcg cacgccgtcg
      181 gcatggcttt ggcgaaaaaa caggcggaag tcagccgtct gaaaacggaa agagaccttt
      241 gcaaaattcc tttccctccc gacagccgaa atcccaacac aggttttcgg ctgttttcgc
      301 cccaaatacc tcctaatttt acccaaatac ccccttaatc ctgcccggat acccgataat
      361 caggcatccg ggctgctttt taggcggcag cggacgcact ta
//
LOCUS       DAWHRS010000306          384 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.306, whole genome shotgun sequence.
ACCESSION   DAWHRS010000306 DAWHRS010000000
VERSION     DAWHRS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.306"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="WIB82_002146"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="WIB82_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ3843376.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
BASE COUNT          115 a           88 c           84 g           97 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgcagt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacgtgaac aaaacttccc gtgagcaatt ggaaatccgc acccacttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactataa aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgc
//
LOCUS       DAWHRS010000307          382 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.307, whole genome shotgun sequence.
ACCESSION   DAWHRS010000307 DAWHRS010000000
VERSION     DAWHRS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.307"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            123..302
                     /locus_tag="WIB82_002147"
     CDS             123..302
                     /locus_tag="WIB82_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3843377.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
BASE COUNT           98 a           71 c          102 g          111 t
ORIGIN      
        1 atttgtactg tcttcggctc gccgccttgt cctgatttaa atttaattca ctatatttgc
       61 ccgtgcgttt gtgataaatt cggcactgta tttgatatga ttttaacaaa tcgggagggg
      121 caatgttttg gtatgtgatt ggcttttgcg cctttgtcgt cgcgctgctg tcgctgtggg
      181 tcaatgccgg cgcgttcggt atgcaggaag acgatactcc gcaatcggat tatgaaaggc
      241 gtttgggctt gggtgcgaag ctgaaaaaca aaaatccgga tacggacgaa aaacggcaat
      301 aaagacaaca aatgccgtct gaaaggtttt cagacggcat ttgctttgga gcggatatag
      361 tggattaaca aaaatcagga ca
//
LOCUS       DAWHRS010000308          374 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.308, whole genome shotgun sequence.
ACCESSION   DAWHRS010000308 DAWHRS010000000
VERSION     DAWHRS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.308"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..298
                     /locus_tag="WIB82_002148"
     CDS             <1..298
                     /locus_tag="WIB82_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3843378.1"
                     /translation="LWAKRQNGSVKWFCGQPVTRNDTATATDVAAANGKTDDKINTKH
                     LPSTCRDDSSTGCIETPRADFKHFQKISRYRVLPESRQMAEKLRHSRKSGNLVR"
BASE COUNT          111 a          109 c           80 g           74 t
ORIGIN      
        1 cctgtgggcc aagcgtcaaa acggttcggt caaatggttc tgcggacagc cggttacgcg
       61 caacgacacc gccaccgcca ccgacgtcgc cgccgccaac ggcaagaccg acgacaaaat
      121 caacaccaag cacctgccgt caacctgccg cgatgattca tctaccggtt gcatagaaac
      181 accacgcgcc gatttcaaac actttcaaaa aatcagccgt taccgagtat tgcccgaatc
      241 acggcaaatg gcagaaaaac ttcgtcattc ccgcaaaagt gggaatctag ttcgttgagt
      301 ttcagttatt tagaataaat tttgaaactt taatcccgtc attcccgcga aagtgggaat
      361 ccagaacgca aaat
//
LOCUS       DAWHRS010000309          336 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.309, whole genome shotgun sequence.
ACCESSION   DAWHRS010000309 DAWHRS010000000
VERSION     DAWHRS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.309"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            144..>336
                     /locus_tag="WIB82_002149"
     CDS             144..>336
                     /locus_tag="WIB82_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ3843379.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIG"
BASE COUNT           87 a           63 c           97 g           89 t
ORIGIN      
        1 aattatgtat ggcgtaaagg aagcgtttta acgccgtatt gggaaaatgt aaagttcttt
       61 atcgggattt ctttaagtat tggcgttttc ggtgtcgtgg cggtagaatc ggggtcagtt
      121 aagaacaatt atcaggaacg gatatggaag aaaaaaacga ctacaccgac gcggcatccg
      181 acaaccgcaa cgggcaggaa attgaagtcg gaattgccgg cgcaggcgac cggatacttg
      241 ccgcgctgct gaaccaattg tttacctttt tggttttgtt ggtgccgttt gccggactga
      301 ttgctttcgc catcaagaat gaaggcagga ttggcg
//
LOCUS       DAWHRS010000310          334 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.310, whole genome shotgun sequence.
ACCESSION   DAWHRS010000310 DAWHRS010000000
VERSION     DAWHRS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.310"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            46..192
                     /locus_tag="WIB82_002150"
                     /pseudo
     CDS             46..192
                     /locus_tag="WIB82_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          101 a          100 c           75 g           58 t
ORIGIN      
        1 atccttttgg ccgaaggtca aaaatcagcc gttaccgagt attacctgaa tcacggcaca
       61 tggccgaaaa acaacacttc tgccggcgtg gcatcttctt caacaatcaa aggcaaatat
      121 gttaaggaag ttacagtcgc aaacggcgtc attaccgcca caatgctttc aagcggcgta
      181 aacaaagaaa tccaaggcaa aaaactctcc ctgtgggcca agcgtcaaga cggttcggta
      241 aaatggttct gcggactgcc ggttacgcgc accgacgcca aagccgacac cgtcgccgcc
      301 gccgccaaga ccgccgacaa catcaacacc aagc
//
LOCUS       DAWHRS010000311          326 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.311, whole genome shotgun sequence.
ACCESSION   DAWHRS010000311 DAWHRS010000000
VERSION     DAWHRS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.311"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           97 a           79 c           81 g           69 t
ORIGIN      
        1 cagaagttgc ccgaaactca aaaaaaacga aaccgaacga accggattcc cgctttcgcg
       61 ggaatgacgg gattttaggt tgctggtatt tatcgggaaa acggaaaccc ctatgccgtc
      121 attcccgcgc aggcgggaat ctaggacgta gaatctaaag caaccgtttt atccgataag
      181 tttctgtacc gaagaatctg gattcccgct ttcgcgggaa tgacggcata tcagtttctg
      241 tgcggacaaa cctggattcc catctgcgta ggaatgacga tgcagaagtt gcccgaaacc
      301 taaaaaactg aaaccgaacg aaccgg
//
LOCUS       DAWHRS010000312          321 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.312, whole genome shotgun sequence.
ACCESSION   DAWHRS010000312 DAWHRS010000000
VERSION     DAWHRS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.312"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           67 a           62 c           90 g          102 t
ORIGIN      
        1 aaacggcttg atttgagttt tggtattttt gcccgacggg gtgaaaaata cagttgctac
       61 ggctcgatga atcgtcagaa atacctgcat cgtcattccc gcgcaggtgg gaatccagac
      121 cggtcggtgc ggaaacttat caggtaaaac ggtttcttga gatttttcgt cttggattcc
      181 cactttcgtg tgaatgacgg aatgtaggtt cgtgggaatg acgtggtgca ggtttccgta
      241 tggatggatt cgtcattccc gcgcaggcgg gaatctagtc tgttcggttt cagttatttt
      301 cgataaatgc ctgttgcttt t
//
LOCUS       DAWHRS010000313          314 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.313, whole genome shotgun sequence.
ACCESSION   DAWHRS010000313 DAWHRS010000000
VERSION     DAWHRS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.313"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           82 a           93 c           72 g           67 t
ORIGIN      
        1 atctcgaaat ccgtcattcc cacacaggcg ggaatccaga cccctgacgc ggcgggaatc
       61 tatcggaaat gactgaaacc ccgagattct agattcccac tttcgtggga atgacggttc
      121 agttgcgttc cgacaacacc gcaatctcga aatccgtcat tcccgcacag gcgggaatcc
      181 agacccctga cgcggcggga atctatcgga aatgactgaa accccgagat tctagattcc
      241 cactttcgtg ggaatggcgg ttcagttgca ttccgacaac accgtaatct tgaaatccgt
      301 cattcccgat aaca
//
LOCUS       DAWHRS010000314          308 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.314, whole genome shotgun sequence.
ACCESSION   DAWHRS010000314 DAWHRS010000000
VERSION     DAWHRS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.314"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            18..212
                     /gene="thiS"
                     /locus_tag="WIB82_002151"
     CDS             18..212
                     /gene="thiS"
                     /locus_tag="WIB82_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ3843380.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT           85 a           90 c           77 g           56 t
ORIGIN      
        1 agaaacatcg agaaaccatg aacatcatct taaacggcgg acccgccgaa cttcacggca
       61 caagcgttgc cgacctcatc gcccaaaccg cgccgcaaaa gccctttgcc gtggcggtca
      121 acaccgtttt catccccaaa ggcgcgtatg cggaaacggt tttgaacgaa aacgacaaaa
      181 tcgatatcgt gcggccggtg gtcggcggct aggcggtttt gccttttcag acgacccctg
      241 tccccaaaac aacgttatgg tggattaact ttaaatcagg acaaggcgac gaagccgcag
      301 acagtacg
//
LOCUS       DAWHRS010000315          295 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03753
            SAMN17286431-rid13901953.denovo.315, whole genome shotgun sequence.
ACCESSION   DAWHRS010000315 DAWHRS010000000
VERSION     DAWHRS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286431
            Sequence Read Archive: SRR13407360
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243139.1
            Genome Coverage       :: 25x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 18:25:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,152
            CDSs (total)                      :: 2,097
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 52 of 97
            Pseudo Genes (internal stop)      :: 12 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286431-rid13901953.denovo.315"
                     /strain="NM03753"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2006"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>295
                     /locus_tag="WIB82_002152"
     CDS             <1..>295
                     /locus_tag="WIB82_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEZ3843381.1"
                     /translation="IYRYLRQDKSNGSTLWQHLRICSKPYRKRYGSTWTRGKVPNRVG
                     IENRPAIVDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLKAED"
BASE COUNT           92 a           89 c           66 g           48 t
ORIGIN      
        1 catttaccgc taccttcgcc aagacaaaag caacggcagc acgttgtggc aacatctcag
       61 aatatgcagc aaaccctacc gcaaacgcta cggcagcaca tggaccagag gcaaagtacc
      121 caaccgtgtc ggcatagaaa accgacccgc tatcgtcgac cagaaatccc gtatcggcga
      181 ttgggaagcc gacaccattg tcggcaaagg acagaaaagc gcattattga ccttggtcga
      241 acgcgttacc cgctacacca tcatctgcaa attggatagc ctcaaagccg aagac
//
